LOCUS       DATRNK010000001        82489 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.001, whole genome shotgun sequence.
ACCESSION   DATRNK010000001 DATRNK010000000
VERSION     DATRNK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 82489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82489
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.001"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            31..306
                     /locus_tag="VBC52_000001"
     CDS             31..306
                     /locus_tag="VBC52_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEO6881614.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFAAVADYLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     gene            503..700
                     /locus_tag="VBC52_000002"
     CDS             503..700
                     /locus_tag="VBC52_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881615.1"
                     /translation="MSEKAILLIILIAFVIIAIIKIALLKLYMNTIKPKVDNEGNIIT
                     KPVKDDLTPEEREEREFDKDW"
     gene            complement(744..1676)
                     /locus_tag="VBC52_000003"
     CDS             complement(744..1676)
                     /locus_tag="VBC52_000003"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEO6881616.1"
                     /translation="MVSLKTEIAGFSFDNCLMNAAGIYCMTKEELLAIENSEAGSFVT
                     KTGTLEAREGNPQPRYADTDWGSINSMGLPNKGIDYYLDFVTELQDQDNSKNHVLSLV
                     GLSPEETHIILKKVENSNYNGLIELNLSCPNVPGKPQIAYDFEMTDLILSEIFSYYQK
                     PLGIKLPPYFDIVHFDQAATIFNKYPLAFINCVNSIGNGLVIDDETVVIKPKNGFGGI
                     GGDFIKPTALANVHAFYKRLNPSIKIIGTGGVKNGRDAFEHILCGASMVQIGTALQKE
                     GPEIFQRVSRELKEIMADKGYQSLEDFRGQLNYL"
     gene            complement(1863..3098)
                     /locus_tag="VBC52_000004"
     CDS             complement(1863..3098)
                     /locus_tag="VBC52_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6881617.1"
                     /translation="MALKELTAKEFESYSGNYDLQSFMQTPEMAKLLKKRGYDITYMG
                     YQIDGKMEIISIVYTIPMTGGLHMEVNSGPAHSNSKYLKHFYKELQNYAKSQGALELL
                     IKPYDTYQEFTGEGKPKGAPNTYLIDDLTSIGYHHDGLHIGYPGGEPDWHYVKNLEGI
                     TPQNLLKSFSKKGRPLVKKAMSFGIKIRVLKREELHIFKDITSSTSDRRDYMDKSLDY
                     YQDFYDSFGDKAEFVIATLNFREYDHNLQLNAKKLEEQITVLDNHHQNNTDSAKYHRQ
                     RTELVNQLASLDKRRKEVEPFIQKFGNQDVVLAGSLFIYSPKETVYLFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRQSTFYNFLGIQGNFDGSDGVLRFKQNFNGYIVRKMGTFRYY
                     PNPLKYKSIQLLKKILRRT"
     gene            complement(3117..4328)
                     /locus_tag="VBC52_000005"
     CDS             complement(3117..4328)
                     /locus_tag="VBC52_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6881618.1"
                     /translation="MYREITAVEHDRFVSESNQTNLLQSSNWPKVKDNWGSQLLGFFD
                     GETQIASASILIKSLPLGFSMLYIPRGPIMDYSNLDIVTKVLKDLKAFGKKQRALFIK
                     CDPLIYLKMVNAKDFENSPDEKEGLIAIDHLQRAGADWTGRTTDLAHTIQPRFQANLY
                     ANQFGLDKMSKKTRQAIRTSKNKGVDIQFGSHELLEDFAELMKKTEDRKGINLRGIDY
                     YQKLLDTYPNNSYITMASLDVAKRLEKIEKECQIAQSERIKSLELNREKKVKQHQGTI
                     DRLNKEIDFLKEAQKAYDRDIIPLAATLTLEFGNTSENIYAGMDDYFKSYSAPIYTWF
                     ETAQRAFERGNIWQNMGGIENDLSGGLYHFKSKFEPIIEEFIGEFNIPVNRLLYKASN
                     YVYALRKKRNS"
     gene            complement(4341..5576)
                     /locus_tag="VBC52_000006"
     CDS             complement(4341..5576)
                     /locus_tag="VBC52_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6881619.1"
                     /translation="MGNKLMYHVTVGISEKEYDAFAIASSQTNLLHSSKWAQVKSNWQ
                     NERLGFYKDDQLVAVASILIKSLPLGFTMLYIPRGPIMDYSNKELVNFVLKTLKNFGR
                     KKRAVFAKFDPALLLRQYHLKEENVAEEIDESRQAIDNLKSAGAQWIGPTKAISETIQ
                     PRFQANIYTKANIEENFPKHTKRLIKDAKHRGVQIYRANIDDLPKFATVVALTENRKG
                     VALRNENYFHQLMTIYGEDAYLYLAKVNLPKRLAQFKEQLLQIQKDLSETPSHQKSRL
                     TRLNQQEASVKQYILEFQEFSKKYPDEPVIAGILSIRFGNVLEMLYAGMDDSFRKFYP
                     QYLLNARVFEDAFKNDIVSANLGGVEGSLNDGLTKFKSNFNPMFEEYIGEFNLAINPL
                     LYKLANLAYTIRKKQRHSH"
     gene            complement(5561..6373)
                     /gene="yidA"
                     /locus_tag="VBC52_000007"
     CDS             complement(5561..6373)
                     /gene="yidA"
                     /locus_tag="VBC52_000007"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEO6881620.1"
                     /translation="MTSIKLVAVDIDGTLLNSKREITPEVAKAVQEAKSKGVKIVIAT
                     GRPIIGVQDLLEELKLNEEGDYVITFNGGLVQDTATGDDIIKETLTYEDYLDFELLAR
                     KLGVHMHAITKEGIYTANRDIGKYTIHEVTLVNMPLFYRTPEEMGDKEIIKLMMIDQP
                     DILDAAIAKIPKKVLDNYTIVKSTPFYLEILPKNVNKGTALLHLAEKMGLTVDQTMAI
                     GDEENDRAMLEVVGNPVVMQNGNPELKKIAKYITKSNEESGVAYALREWVIN"
     gene            complement(6444..7760)
                     /locus_tag="VBC52_000008"
     CDS             complement(6444..7760)
                     /locus_tag="VBC52_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6881621.1"
                     /translation="MNEKVFRDPVHTYIHVNNQVIYDLINTKEFQRLRRIKQTSTTSF
                     TFHGAEHSRFSHCLGVYELARKVTEIFDEHYSDLWNKNESLLTMAAALLHDIGHGAYS
                     HTFERLFNTDHEAYTQEIITNPTTEINAILRKVAPDFPDKVASVINHSYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYYTAASYGQFDLTRILRVIRPTDSGIAFARNGMHAVEDYIVS
                     RFQMYMQVYFHPASRAMELLLQNLLKRARFLFDTHRDFFEQTSPNLIPFFTDQYDLQD
                     YLALDDGVMNTYFQSWMQADDNILADLANRFINRKVFKSITFEESDKENLVKMKELVS
                     QVGFDPDYYTGVHANFDLPYDVYRPEHSNPRTEIQIIQKNGQLAELSSLSPIVKALTG
                     SNYGDQRFYFPKEMLTLDSLFSSTKEEFQSYITNEHLTLTKDNSHS"
     gene            7824..8222
                     /locus_tag="VBC52_000009"
     CDS             7824..8222
                     /locus_tag="VBC52_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEO6881622.1"
                     /translation="MREVMKLEINNNIQIDNETEMIHEIHDCQFIEKGSYVYLNYINA
                     EGERVVIKANHEELLMTRFSNPKSVMRFHRETPALVNIPTPLGVQHLITETSHYQFDL
                     SQQRLHINYVLKQTETGDCFANYELRIQWY"
     gene            8566..11250
                     /locus_tag="VBC52_000010"
     CDS             8566..11250
                     /locus_tag="VBC52_000010"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HEO6881623.1"
                     /translation="MLKEQKKSLFYTQGQEEVLTSLESSREGLSTTEAKNRLEMYGRN
                     ELEEGKKRSLIAKFFDQFKDLMIIILLVAAALSVITEGMHGLTDALIILAVVILNAAF
                     GVYQEGQAEAAIEALKDMSSPIARVRRDGHTIEVDSKELVPGDLVMLEAGDVVPADLR
                     LLEAASLKIEEAALTGESVPVEKDISQVVAEDAGIGDRVNMAYQNSNVTYGRGYGVVT
                     NTGMYTEVGKIADMLANADESETPLKQSLVQLSKLLTYLIVIIAVITFLVGIFVRKEG
                     WIEGLMTSVALAVAAIPEGLPAIVTIVLSMGTKTLAKRNSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGVLQSSSEEISVDNNTLRIMNFSNDTKIDPSGKLIGDP
                     TETALVQFGLDKNFDVREVLKNEPRVAELPFDSDRKLMSTIHKESDGRYFIAVKGAPD
                     QLLKRVTKIEDNGLVRDITAEDKEAILNTNKELAKQALRVLMMAYKYETQIPTLETDI
                     VESDLVFSGLVGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGIIDA
                     NDTEDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNDGKVVAMTGD
                     GVNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKS
                     IQYLLSANMAEVFTIFFATLLGWDVLAPVHLLWINLVTDTLPAIALGVEPAEPGVMTH
                     KPRGRQSNFFDGGVMGAIIYQGILQTILVLGVYGWALMYPEHAGYRMIHADALTMAFA
                     TLGLIQLVHAFNVKSVYQSIFTVGAFKNRTFNWSIPVAFILLMVTIVVPGFNKLFHVT
                     HLSSTQWLTVVIGSLLMVVLTEIVKFIQRKLGQDEKAI"
     gene            complement(11295..12155)
                     /locus_tag="VBC52_000011"
     CDS             complement(11295..12155)
                     /locus_tag="VBC52_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO6881624.1"
                     /translation="MTKLAIMSDLHIDLNHFSTFETKTLINLLKKEKIDHLHIAGDIS
                     NHFTKDTLPFINNLKKHIKLSYNLGNHDMLDLTETEIQRLDFQTYRFDKKMLLAFHGW
                     YDYSFSNNRDIKDVEKLKKTFWFDRRLKRPNNDVTIQASILKRLDEILAKVDSSNIII
                     AMHFVPHKQFTMTHPRFSPFNAFLGSQAYHDLFQKYHIKDVVFGHAHRSFGDVKIGET
                     TYHSRPLGYIREWNLTIDFVNQNPNHNPNLTWNLSKRYNAVKHLDSFENYPKKYFEDE
                     LRNSMTIFDC"
     gene            12307..14238
                     /locus_tag="VBC52_000012"
     CDS             12307..14238
                     /locus_tag="VBC52_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HEO6881625.1"
                     /translation="MSNFYKLLKEKFPRKEDIVTEMINLEAICQLPKGTEYFISDLHG
                     EYDAVDYLLRTGAGSIRAKLLDCFDWQKIVAVDLDDFCILLYYPKEKLAFDKMNLSAS
                     AYKTKLWEMIPLQIQVLKYFSSKYTKSKVRKQLSGKFAYIIEELLAEIDRNPEKKSYF
                     DTIIEKLFELDQVEDLIIVLSQTIQVLIIDHLHVVGDIYDRGRYPDRILNRLMAFPNL
                     DIQWGNHDVTWMGAASGSYLCMVNVIRIAARYNNITLIEDRYGINLRRLVDYSRRYYE
                     PLPSFVPILDGEEMTHPDELDLLNMIQQATAILQFKLEAQLIDRRPEFQMHNRQLINQ
                     VNYKDLSISIKEVVHQLKDFNSRCIDSKNPSRLTSEEEELLQQLMIAFQTSESLKKHI
                     DFLFEKGSMYLTYNDNLLFHGCIPMHSNGDFKSFKIAGKTYGGRDLLDLFESQIRLAY
                     ARPEKHDDLATDIIWYLWCGENSSLFGKNAMTTFERYYVSDKVTHQERKNPYFKLRDK
                     DDICTALLQEFDLPKFGHIVNGHTPVKEKNGEQPIKANGKMLVIDGGFAKGYQKNTGL
                     AGYTLIYNSYGIQLISHLPFTSIEEVLSGTNYIIDTKRLVEEAKDRILVKDTTIGQKL
                     TKEIKDLDHLYRHFQEYDD"
     gene            14328..15452
                     /gene="queG"
                     /locus_tag="VBC52_000013"
     CDS             14328..15452
                     /gene="queG"
                     /locus_tag="VBC52_000013"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEO6881626.1"
                     /translation="MDIKAEIQKLAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRIYPERLLESAKTIISIGVAYPHKLPQQPQKTSYKRGKITPNSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNPLATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEILEFMSNLTLKDEDSRKELELIRHKWQF"
     gene            15521..16619
                     /gene="prfB"
                     /locus_tag="VBC52_000014"
     CDS             join(15521..15592,15594..16619)
                     /gene="prfB"
                     /locus_tag="VBC52_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEO6881627.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENQMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            16638..17330
                     /gene="ftsE"
                     /locus_tag="VBC52_000015"
     CDS             16638..17330
                     /gene="ftsE"
                     /locus_tag="VBC52_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEO6881628.1"
                     /translation="MALIEMSGVTKKYRRSTTALRNLNLSIQQGEFVYLVGPSGAGKS
                     SLIRLLYREEKLSSGRLKVGEFNLNKLKRRQIPILRRSIGVVFQDYKLLPTKTVYENV
                     AFAMQVIGAKRRHIKKRVPEVLELVGLKHKMRSFPTQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEIAWEIMHLLERINLQGTTVLMATHNSQIVNTLRHRVIEIEAGSVI
                     RDEEKGEYGYHD"
     gene            17314..18243
                     /locus_tag="VBC52_000016"
     CDS             17314..18243
                     /locus_tag="VBC52_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6881629.1"
                     /translation="MVIMINFFRHFWESLKNLKRNFWMTFASVTSVTITLLLVGLFSS
                     VLLNVEKLTTDVSGNFTISAFLNVDSTDAQKQVKDKDGKLKDNPDYHKVYDKIKRISG
                     VEKVTYSSKAEQLKEVQKEYGSDVIDDTYKDALLDVYVVGTSSAKVSKSVSEAIGRIE
                     GVDYTKEPIDSTKLSNLTDNIRIWGFGGVALLIVLAIFLISNTIRMSIMSRRTDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGILGAIVPSLIFYFGYQFVFNKFNPKFETSHVSLYPM
                     DIMVPAIIGGMVIIGIIIGSLGSVLSMRRYLKI"
     gene            complement(18296..19006)
                     /locus_tag="VBC52_000017"
     CDS             complement(18296..19006)
                     /locus_tag="VBC52_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6881630.1"
                     /translation="MNIKRHSIFLWLTTSILAILMAVGMTIYFNTYQASPLARRIAKT
                     ATNHKNYLLFRSKGKVKANIIFYQGALVEEEAYSQLARDLADKGYNTYILKTPLNLPV
                     LSPHKAKTIINQNHLTNVYLAGHSLGGVVASQNAKVAPVRGLILLASYPSRKSDLSHK
                     KLRVLSITASNDHILNWEKYEEAKKRLPNSSTFRTIVGGNHSRFGNYGHQKGDGKATL
                     SHKSSEKQLATFISNFIH"
     gene            complement(19003..19701)
                     /locus_tag="VBC52_000018"
     CDS             complement(19003..19701)
                     /locus_tag="VBC52_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001907086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6881631.1"
                     /translation="MPFIFRHSFFNKVLIFWYTIIMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDTFDNPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIINVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPSHYSVHPGHGMN
                     TTIGHEKNFNPFFQ"
     gene            19869..20633
                     /locus_tag="VBC52_000019"
     CDS             19869..20633
                     /locus_tag="VBC52_000019"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEO6881632.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGVLDYNEETAQAAVD
                     KLSPEDAVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGVAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            20741..23248
                     /locus_tag="VBC52_000020"
     CDS             20741..23248
                     /locus_tag="VBC52_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEO6881633.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLAKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEQLFLEGCELRTPRIDTVELSQVFYPCLEKYSLGALAESLNIEL
                     TDAHTAIADARATAQLFIKLKAKISSLPKEVLETILTFADNLLFESYLLIEEAYQEAD
                     FVNPKEYYFWQGLVLKKEKAVGKPKKLSSDFQVNMALLGMDARPKQVVFADLVKAHFN
                     DQTTTFLEAQPGLGKTYGYLLPLLDQSQKQQIIVSVPTKILQDQIMAKEIKHIQELFH
                     IPCHSIKGPRNYLKLDAFYKSLQVQDRNRLINRFKMQLLVWLTETTTGDLDEIKQKQR
                     LESYFDQLKHDGEVTQSSLFYDLDFWKRSYDKVAQSQLVIINHAYFLERVQDDKDFAK
                     GKVLVFDEAQKLVLGLENFSRGQLDISHQLQVIQKIIDSSIPLLQKRLLESISYELSH
                     AVELFYRHNSFEFSETWLKRLKNSINALEVVGLDELQTFFTATYTNYWFETDKVNEKR
                     LTILRGAREDFLKFSKFLPPTKKTYMISATLQISPKVYLSDLLGGFSSISTEKIAHEK
                     NANQKVWIDTSMPNILDLSPEQYAYEIAKRLQDIMTLKQPTLVLLTSKQTMFMVSDYL
                     DKWEIKHLTQDKNGLAYNVKKRFDRGESNLLLGTGSFWEGVDFVHRDRLIEVITRLPF
                     DNPKDYFIQKLSQSLTKEGKNFFYDYSLPMTVLKLKQALGRTTRREEQKSAVIILDSR
                     LVIKSYGQTIMHSLGRDFEISKEKINKVLTEMAKFLI"
     gene            23334..24527
                     /locus_tag="VBC52_000021"
     CDS             23334..24527
                     /locus_tag="VBC52_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6881634.1"
                     /translation="MTYLSERALNMEESVTLAAGAKARELRVQGRDILSLTLGEPDFA
                     TPKNIQQAAIEAITDGRASFYTPSSGLPELKSAINAYFERFYGYSLKPNQVVVGTGAK
                     FILYTFFMTVLNPGDEVIIPTPYWVSYADQIKMAEGKPVFVTAKEVNHFKVTVEQLEA
                     VRTDKTKVILLNSPSNPTGMIYKAEELEAIGNWAVEHDILILADDIYGRLVYNGNIFT
                     PISSLSESIRNQTIVINGVSKTYAMTGWRVGFAVGNHDIIAAMSKVVSQTTSNLTAVS
                     QYATIEALNGSQESFEKMRLAFEERLNIIYPLLCQVPGFEVVKPQGAFYLFPNVTKAM
                     EMKGYTDVTAFTDAILEEVGLALVTGAGFGAPENVRLSYATDLETLKEAVRRLHVFMG
                     SNEIN"
     gene            24548..25894
                     /gene="asnS"
                     /locus_tag="VBC52_000022"
     CDS             24548..25894
                     /gene="asnS"
                     /locus_tag="VBC52_000022"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEO6881635.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEVIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYSTYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLNRAPQALDILERDVE
                     ALKRYIAEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDDYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIKP"
     gene            complement(25934..26491)
                     /locus_tag="VBC52_000023"
     CDS             complement(25934..26491)
                     /locus_tag="VBC52_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6881636.1"
                     /translation="MTKSPARLISFISIAIAINLVGANLALFLRLPIYLDTIGTLLIA
                     VILGPWYAASTAFLSALINWMTTDIFSLYYSPVAIVVAIITGILIKRNCKPSSLLWKS
                     LIISLPGTIIASVITVILFKGITSSGSSIIAQFLHGIGLDMTSSLILVQVGTDYMDRL
                     ISLILVFSTITLLKKHSPNLINQRF"
     gene            complement(26488..27471)
                     /locus_tag="VBC52_000024"
     CDS             complement(26488..27471)
                     /locus_tag="VBC52_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEO6881637.1"
                     /translation="MNKEKIIIDCDPGIDDTLALMYAIQHPKLEVVAITITAGNSPVE
                     LGLKNTFVTLELLNRHDIPVYVGDNLPLQREFVSAQDTHGMDGLGENNFTLAQPIIFQ
                     EESADCFLANYFEHKNDTSIIALGPLTNIARALQTNPKLGKHCKRFISMGGSFKSHGN
                     CSPVAEYNYWCDPHAAQYVFENLDKKIEMVGLDITRHIVLTPNHLSYMERINPDVSSF
                     IQKITKFYFDFHWQYEHIIGCVINDPLAIAYFVNENIATGFDSYTDVACHGIAMGQTI
                     VDQYHFYKKDANSKILTSVNTNLFWNDFMTVLLHAQNSVIIEDLEMLGLNK"
     regulatory      complement(27615..27659)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            complement(27717..28130)
                     /locus_tag="VBC52_000025"
     CDS             complement(27717..28130)
                     /locus_tag="VBC52_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEO6881638.1"
                     /translation="MLYEVTSSNTQGVDGKVYLSNGKIVETNHPLNHLPGFNPEELIA
                     LAWSTCLNATIKAILEQKGFKDLKSRVDVTCQLMKEKQVGKGFYFQVNAVASIEKLSL
                     SDSKLIVNKAHSRCPISKLISNAKTINLDTVKWSV"
     gene            28284..29174
                     /gene="rapZ"
                     /locus_tag="VBC52_000026"
     CDS             28284..29174
                     /gene="rapZ"
                     /locus_tag="VBC52_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEO6881639.1"
                     /translation="MSDEQIKLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPTLVPK
                     FLELAAQSGDTSKIAMVVDMRSRLFFREINSILDSLEINDNINFKILFLDATDTELVS
                     RYKETRRSHPLAADGRVLDGISLERELLAPLKSMSQNVVDTSELTPRQLRKVISKEFS
                     NQDSQSSFRIEVMSFGFKYGIPLDADLVFDVRFLPNPYYKPELRDKTGLDTEVYDYVM
                     SFDESDDFYDHLLALIKPILPGYQNEGKSVLTVAIGCTGGQHRSTAFAHRLSEDLKAD
                     WTVNESHRDKNKRKETVNRS"
     gene            29171..30145
                     /locus_tag="VBC52_000027"
     CDS             29171..30145
                     /locus_tag="VBC52_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEO6881640.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLRLEDVEITAVVTVADDGGSSGE
                     LRSVMQLTPPGDLRNVLVALSDMPKFYEQIFQYRFAEGDGDFAGHPLGNLIIAGVAEM
                     QGSTYNAMQSLTQFFHTTGKIYPSSEHPLTLHAVFKDGHEVVGESQIADYKGMIDHVY
                     VTNTYNEETPTASRKVVDAILESDMIVLGPGSLFTSILPNLVIPEIKQALLETRAEVA
                     YVCNIMTQRGETEHFTDADHVEVLKRHLGQDAIDTVLVNIEKVPESYMENNHFDEYLV
                     QVEHDFSGLRKHARRVISSNFLKLEKGGAFHHGDFVVEELMNLVGTHL"
     gene            30142..31053
                     /gene="whiA"
                     /locus_tag="VBC52_000028"
     CDS             30142..31053
                     /gene="whiA"
                     /locus_tag="VBC52_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000011317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEO6881641.1"
                     /translation="MSFTVKVKEELLGHKSENKMELSAIIKMSGSLGLANHGLNLSIT
                     TENAKIARHIYSMLEEHYHLQPEIKYHQKTNLRKNRVYTVFIEEKVDVILADLKLADA
                     FFGIETGIEHSILDNDENGRAYLRGAFLSTGTVREPDSGKYQLEIFSVYLDHAQDLAN
                     LMKKFMLDAKVIEHKHGAVTYLQKAEDIMDFLIVIDAMEARDAFEEIKMIRETRNDIN
                     RANNVETANIARTITASMKTINNIIKIMDTIGFDALPSDLRQVAQVRVAHPDYSIQQI
                     ADSLETPLSKSGVNHRLRKINKIADEL"
     gene            31068..32465
                     /locus_tag="VBC52_000029"
     CDS             31068..32465
                     /locus_tag="VBC52_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEO6881642.1"
                     /translation="MACTTILVGKKASYDGSTMIARTEDSVNGDFTPKKLKVMTSKDQ
                     PRHYKSVLSNFEVDLPDNPLPYTSVPDALGKDGIWGEAGINSKNVAMSATETITTNSR
                     VLGADPLVSDGIGEEDILTLVLPYIQSAREGVERLGAILEKYGTYESNGIAFSDTEEI
                     WWLETIGGHHWIARRVPDDVYVTNPNQLGIDHFEFNNCDDYMCSSDLKEFIEQYHLDL
                     TYSNEHFNPRYAFGSQRDKDRHYNTPRSWAMQRFLNPEIEQDPRSLFIPWCQKPYRKI
                     TVEDIKYVLSDHYQDSVYDPYGPEGDAVSRRAFRSIGINRTSQTSILQLRPNKSLETT
                     GVQWLSYGSMPFATMVPLFTQVETVPNYFSNTTKDASTDNFYWTNRLIAALADPHFYQ
                     HEADIESYIERTMAQGHAHINGVDREVAENKEIDFQQKNQEMSDYIQKESQELLNRIL
                     FDASNLMTNRFSMGD"
     gene            32607..34127
                     /locus_tag="VBC52_000030"
     CDS             32607..34127
                     /locus_tag="VBC52_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEO6881643.1"
                     /translation="MRKKFLLLMSFVAMFAAWQLVQVKQVWADSKLKVVTTFYPVYEF
                     TKNVVGDKADVSMLIKAGTEPHDFEPSTKNIAAIQDSNAFVYMDDNMETWAPKVAKSV
                     KSKKVTTIKGTGDMLLTKGVEEEGEEHEGHGHEGHHHELDPHVWLSPERAISVVENIR
                     NKFVKAYPKDAASFNKNADAYIAKLKELDKEYKNGLSNAKQKSFVTQHAAFGYMALDY
                     GLNQVPIAGLTPDAEPSSKRLGELAKYIKKYNINYIYFEENASNKVAKTLADEVGVKT
                     AVLSPLEGLSKKEMAAGEDYFSVMRRNLKVLKKTTDVAGKEVAPEEDKTKTVETGYFK
                     TKDVKDRKLTDYSGNWQSVYPLLQDGTLDPVWDYKAKSKKDMTAAEYKKYYTAGYKTD
                     VESIKIDGKKHQMTFVRNGKSQTFTYKYAGYKILTYKKGNRGVRYLFEAKEKDAGQFK
                     YIQFSDHGIKPNKAEHFHIFWGSESQEKLFEEMENWPTYFPAKMSGREVAQDLMSH"
     gene            complement(34240..34500)
                     /locus_tag="VBC52_000031"
     CDS             complement(34240..34500)
                     /locus_tag="VBC52_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEO6881644.1"
                     /translation="MKKDIHPDYRPVVFLDTTTGYKFLSGSTKSTKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAQ"
     gene            complement(34609..35544)
                     /locus_tag="VBC52_000032"
     CDS             complement(34609..35544)
                     /locus_tag="VBC52_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEO6881645.1"
                     /translation="MIIFQQILDKIKEYDTIIIHRHMRPDPDALGSQIGLRDIIRHNF
                     PKKKVLATGFDEPTLAWIAKMDQVTDQDYQGALVVVTDTANTPRIDDERYKKGDFLIK
                     IDHHPNDEVYGDLSYVDTNASSASEIVTDFALSCDLLLSTSAARVLYNGIVGDTGRFL
                     YPATTSKTLKIASKLREFDFDFSAMARQMDSFPFKIAKLQGFIFEQLKIDKNGAACVT
                     LTQEDLKRFDVTDAETAAIVGVPGKIDIVESWAIFVEQSDGHYRVRLRSKSHIINEIA
                     KRHNGGGHPLASGANSYSLEENQAIYQEIQEILSL"
     gene            35810..36832
                     /locus_tag="VBC52_000033"
     CDS             35810..36832
                     /locus_tag="VBC52_000033"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEO6881646.1"
                     /translation="MTQAVLKELAKAELHCHLDGSLSLPAIRKLANMADIILPSSDKE
                     LRKYVIAPAQTESLVDYLKTFEFIRPLLQTKEALRFAAYDVARQAALENVIYIEIRFA
                     PELSMDKGLTASDTVLAVLEGLADAQKEFNIVARALVCGMRQSSHKTTKDIIKHIVDL
                     APKGLVGFDFAGDEFSYPTDSLVDLIQEVKRSGYPMTLHAGECGCAKHIADSLNLGIK
                     RMGHVTALTGQRDLIKRFVEEDAVAEMCLTSNLQTKAASSIQSFPYQELYDAGGKITI
                     NTDNRTVSDTNLTKEYSLFVTYFGTKIEDFLVFNQNAVKASFTSDSEKDTLLHKLQEN
                     YDSYLK"
     gene            36891..37334
                     /locus_tag="VBC52_000034"
     CDS             36891..37334
                     /locus_tag="VBC52_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEO6881647.1"
                     /translation="MALAKIVYASMTGNTEEIADIVADKLRDLGLDVEVEECTMVDAA
                     DFEDADIAIVATYTYGDGDLPDEIVDFYEDLAEVDLSGKVYGVVGSGDTFYDYFCKSV
                     DEFEAQFALTGAQKGADCVKVDLAAEDEDIENLEAFAEEIASKLN"
     gene            37411..37686
                     /locus_tag="VBC52_000035"
     CDS             37411..37686
                     /locus_tag="VBC52_000035"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEO6881648.1"
                     /translation="MELEEIRQEIDEIDQQLVSLLETRMGLILEVIAFKKKHRLPVLD
                     NNRENEVLNNVLKKVQNHQFDDVIRATFKDIMTESRVYQKENIVDGD"
     gene            37679..38875
                     /locus_tag="VBC52_000036"
     CDS             37679..38875
                     /locus_tag="VBC52_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEO6881649.1"
                     /translation="MIKRFDNYAQYVLVLGFSLLIGGVVGAITAVFGRVLLFLTAFRS
                     DYIAYLLPFLSIVGLFIVFVYQKFGGKSVKGMGLVFEVGHGNEETIPKRLVPLVILTT
                     WLTHLFGGSAGREGVAVQIGATVSHYFQKYCRLQNASQLFLVMGMAAGFAGLFQTPLA
                     ATFFAIEVLVVGRLMVSYVLPSLIAALTANFVSHSLGLEKFSHSIATSMALTPDIILK
                     LLVLGLCFGLCGNLFAYLLAKAKLIASSRLLNPYKRIFTLGLLATFLLFIFHFGRYSG
                     LGTNLIEASFTNKNLYDYDWLLKLCLTVITLAAGYQGGEVTPLFAIGASLGVIIAPIL
                     GLPVILVAALGYTSVFGSATNTLLGPILIGGEVFGFANTPYFVIVCLVAYSISHAHTI
                     YGAQSR"
     gene            39455..39802
                     /gene="rplS"
                     /locus_tag="VBC52_000037"
     CDS             39455..39802
                     /gene="rplS"
                     /locus_tag="VBC52_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEO6881650.1"
                     /translation="MNPLIQSLTEGQLRSDIPEFRAGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            39913..39984
                     /locus_tag="VBC52_000038"
     tRNA            39913..39984
                     /locus_tag="VBC52_000038"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39946..39948,aa:Arg,seq:cct)
     gene            complement(40027..41106)
                     /locus_tag="VBC52_000039"
     CDS             complement(40027..41106)
                     /locus_tag="VBC52_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6881651.1"
                     /translation="MWIEELANGKFKYIERYTDPLTNKYKKVSVTLDKNSSQAQKKAG
                     LILQEKIEDRLAIRNHSEMTYGELKKEYLKQWIPTVKDSTKRGYLVSDSHIATVLPDD
                     TIINKLTKRDIRLIIDKLLKHNSYHVTHKCRKRLHAIFSYAIQMDYMTSNPTENVLVP
                     KPKDDYKPEKVLYLTSNEVYDLCNRMIDNDEQTLADIVLFMFLTGVRYGELACLTYDK
                     IDFENKEILINATYDFNTREITTTKTKKSTRKISVSDNILDIVNRQKKTSSFVFPNSN
                     GVPILNAYINKRLKIYGDYHTHLFRHSHISFLAEKGIPLNAIMDRVGHSDPKTTLSIY
                     SHTTVNMKEIINKQTAPFVPLLKSE"
     gene            complement(41238..41708)
                     /locus_tag="VBC52_000040"
     CDS             complement(41238..41708)
                     /locus_tag="VBC52_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEO6881652.1"
                     /translation="MKIGMRKSSLKKSFKARTTGKLKRQIKKKVIPGYGKKGMGILHP
                     KKALYNKVYRKTTFGGISGINQSYSGKSKTIQYEYHEVFLKEHRVNKLAYCLLAFFLG
                     SIGGQYFYMKEFKKGLLCFFLSWTTVPIFIGFYQAIKAIFEPFNNDDTISIYTK"
     gene            complement(41738..42037)
                     /locus_tag="VBC52_000041"
     CDS             complement(41738..42037)
                     /locus_tag="VBC52_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881653.1"
                     /translation="MAIFGGEKLSREEKQKQKIQKYLSQRGIDNLNEKSNVQVRRVIN
                     DLAGNGFFKAGMALSFAKAEEQAKVTYLSALVEQNWILISQNQEILDELKKINNN"
     gene            complement(42120..42899)
                     /locus_tag="VBC52_000042"
     CDS             complement(42120..42899)
                     /locus_tag="VBC52_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6881654.1"
                     /translation="MARGRGKLTPQDLEYMKIISNNINHFLITQNRKQIDVSRGTGIP
                     PSTLTGYIKGTSLPIPGNVQKIADFFGVLKSDIDPRFKSSKTSTAPNINNKVALLDEE
                     LKEPRHSDWISYGENLLFEQNTVEDSKNTVVELFSYNYYDHAASAGTGQYLNDVQVET
                     IELPVDYDADFVIPVYGDSMEPEYHSGDYVFVKLSVELVDGDIGVFEYYGDAYIKQLL
                     IHAEGAFLHSLNDKYSDIQIDRDSDFRIIGKVVGNFMPKEY"
     gene            complement(43424..43621)
                     /locus_tag="VBC52_000043"
     CDS             complement(43424..43621)
                     /locus_tag="VBC52_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881655.1"
                     /translation="MKISNSKDLALAIVASSSPTLSIEDKIKLYEDSMEAIKKHNLPF
                     IEAEKESAKMSRDALTKVFGR"
     gene            43680..43838
                     /locus_tag="VBC52_000044"
     CDS             43680..43838
                     /locus_tag="VBC52_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881656.1"
                     /translation="MNWKKLMFGDLEHTFTSRNGKEKTSIEFEGGVLPALLVLGGITW
                     LIAWFITK"
     gene            43879..44016
                     /locus_tag="VBC52_000045"
     CDS             43879..44016
                     /locus_tag="VBC52_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881657.1"
                     /translation="MINLEEKVQKIEQNEEENTTSISFLRGYIKGLEERISRLERSKG
                     C"
     gene            complement(43985..44680)
                     /locus_tag="VBC52_000046"
     CDS             complement(43985..44680)
                     /locus_tag="VBC52_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881658.1"
                     /translation="MSKKSCFVVSAIGEESSEIRNHSDSVLNYIIKPALIEKYQVTRA
                     DELYHSDRIDDKIFDALSTADLVIVDITGNNPNVFLELGFRKALNLPTIFLRQKTDED
                     IPFDIRTINTIHYDLKNSESKVVLDSVQETIRRIQKTEENIDFSIIHEPNDQSASVQD
                     IIQLKTSINNIYDAIENLSDKIENNSTQKRPMTQEDLIMMAFQEPEKLEKIFELQTKY
                     PNAFNSPSNALND"
     gene            44735..44944
                     /locus_tag="VBC52_000047"
     CDS             44735..44944
                     /locus_tag="VBC52_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881659.1"
                     /translation="MRPKKYPYSQKNYPAPKLHNIIETDNHFLIDDKRIHYVIEDSVK
                     TKTLGDGYVEVTLSIIAKSFTKSQG"
     gene            45176..45391
                     /locus_tag="VBC52_000048"
     CDS             45176..45391
                     /locus_tag="VBC52_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6881660.1"
                     /translation="MQQISLRALRVNYNLSAKEVAKTLEIHYQTLLKYENDSSKIPHD
                     LLKKLADFYNVDTNYIFLGKKYELIQN"
     gene            45413..46333
                     /locus_tag="VBC52_000049"
     CDS             45413..46333
                     /locus_tag="VBC52_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEO6881661.1"
                     /translation="MKELALSNNLQQIELDLRQENEQIGKSIWKIGCMLKHVKENDLT
                     HGQFMDWYKNLGYNKNFVSKAITIADKLSNFPTLGNIGTEALYLIATLPDNQKQEQIE
                     RIESGDNPTVRELQEIKRENNRLKSENARLEQQKENLAEQALSAKIVEKEVIKEVIPD
                     DYESTKQLNQTLLGKNKELSKMVDDALQHEEYLKSQLKEFYAKRDEVNHKSAKYDELT
                     EAIKQSEGKLNSYQKKIASYKNITELLKKGDLLLLEMSGLIYADETHYIQRDGLIKQE
                     FDSLVDRGLKLFNDLDMKRKNTEILEGEVL"
     gene            46330..46785
                     /locus_tag="VBC52_000050"
     CDS             46330..46785
                     /locus_tag="VBC52_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEO6881662.1"
                     /translation="MNELTTTQQLIEMSKMQTVTLEKVDNLEKGLLQAQNDIQEIMDT
                     SYLHPGIINMITKKRRKRVIDCMGGKSAKAYKTFKVDEEGRKHRFSSEVFREMELDFK
                     AEFDLNSYAELSKSKKEEALEYIAMWEPCTNTKRKINLLNKQTELELIG"
     gene            46817..46975
                     /locus_tag="VBC52_000051"
     CDS             46817..46975
                     /locus_tag="VBC52_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881663.1"
                     /translation="MEENIKQLITENILEFLGSDYGKGFLTGIKIAVEIIAKQIPTPS
                     EEDIGHDS"
     gene            47102..47248
                     /locus_tag="VBC52_000052"
     CDS             47102..47248
                     /locus_tag="VBC52_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881664.1"
                     /translation="MITILKEINQTLKEILAELKEPTVVTVDSEKLSNTLTTEQKIRR
                     LSGQ"
     gene            complement(47211..47519)
                     /locus_tag="VBC52_000053"
     CDS             complement(47211..47519)
                     /locus_tag="VBC52_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881665.1"
                     /translation="MNDVLKTNLIADVAIFSEKSNCKLNVITASGIFTGTLLPENPDK
                     AKYAHVLEFLEYRKENKDDNERFMLLVDATLSTSKESTLNLPFVVLFIDQIIGVSSVQ
                     "
     gene            47625..47855
                     /locus_tag="VBC52_000054"
     CDS             47625..47855
                     /locus_tag="VBC52_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881666.1"
                     /translation="MLAKLKSGIEVPYEELWLNDNDLSEFIGKSFDQTQRLLRKMYKD
                     RNYRKYIDKVGGRSTKVKKFEEWRKLQNEKII"
     gene            47839..47985
                     /locus_tag="VBC52_000055"
     CDS             47839..47985
                     /locus_tag="VBC52_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881667.1"
                     /translation="MKKLFNFIFTKPKKEEPKPKRTIETHGWEVGAKAYDDFHNYMKG
                     LKHG"
     gene            complement(48263..48355)
                     /locus_tag="VBC52_000056"
     CDS             complement(48263..48355)
                     /locus_tag="VBC52_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019781509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEO6881668.1"
                     /translation="MCEIFFTTIIAPLLVGIVLLLVQKWLDNDD"
     gene            48920..49090
                     /locus_tag="VBC52_000057"
     CDS             48920..49090
                     /locus_tag="VBC52_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881669.1"
                     /translation="MAKMGRPTSNPRDKYIGVRASKNEVEMLDFCTEKTGKSKTDILM
                     EGLEKVYNELKG"
     gene            49267..49707
                     /locus_tag="VBC52_000058"
     CDS             49267..49707
                     /locus_tag="VBC52_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881670.1"
                     /translation="MKTYTLTEEELNELVAERMKQAKEKRTPQGLFKDVGFDDELIPI
                     NNKYPKVLKKLNRERAYKPEKHVFNQTPKVFGVDNEISYSKITTHDVHNHIRLLVLNV
                     FGKSQNKEVLPEEYDQAIELYNQLKEWFVSSYDKRLEGLVLEDD"
     gene            49824..50738
                     /locus_tag="VBC52_000059"
     CDS             49824..50738
                     /locus_tag="VBC52_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HEO6881671.1"
                     /translation="MGKEKTKIYFWLKFDKKFFENIFIKRLKKMAGGDTMTVLYIRLM
                     LESLETDCILYYEGYFDNLVEELAIKLEVSEDDINMTLAYFTKCGLIQIDGSGNAQMP
                     QAKAMLESETNWAKYKRETRKIGQIPTDVQPMSNQCPTEIEIDIEKKKDKERELDKEK
                     EYIVEQSPTEYLFPDWLEEKYVEQVKKGNPKNFDYRIPIAYLNQKMNSNYKFVKTNTD
                     LVKARLKDSYTLEDFKAVIDKKCSEWVNSDMEKYLRPSTLFNASKFESYLNQPEVAKS
                     DYYQKQQGQRFSQAELDELKKPDPKYGF"
     gene            50748..51590
                     /locus_tag="VBC52_000060"
     CDS             50748..51590
                     /locus_tag="VBC52_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6881672.1"
                     /translation="MAFGLMTRESMLENGIIRDTGKTCEKHEMPIYARKMPNHGNRET
                     EFCWQCTTEYIQTKSNAVDIAYNNQSLLAKGYKVFYKESVLSKEIASATLKNYKEHSA
                     VDTKALNYAKRITRDYVKGMEGNSLLQGPPGVGKSHLSMSIAKNINEMFKSYNQSKSV
                     IFVSVPLLSGLVKDTFDYDDKKNSKYSQERMSKLLINCDYLILDDLGKESTTGNTIKS
                     ASGWTYTFLFNILDNRTNTIINTNFSRAELMKIYDAAFVDRIIKGAKNNIFKYPDNAE
                     SKRF"
     gene            51590..51736
                     /locus_tag="VBC52_000061"
     CDS             51590..51736
                     /locus_tag="VBC52_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881673.1"
                     /translation="MELTLTTFFSLSEEHAAKIMALDEPSRNKKIEEYRQLRLRRGRI
                     DFGK"
     gene            51726..52001
                     /locus_tag="VBC52_000062"
     CDS             51726..52001
                     /locus_tag="VBC52_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881674.1"
                     /translation="MENRPDLKLVAELENRIKDLKIENEILKSKNIDLSEDVKHLKYK
                     CFEKDSSIVDIMVNNNDLRKENNELREMFDFFKDRLEKFVGSSHGRN"
     gene            51988..52242
                     /locus_tag="VBC52_000063"
     CDS             51988..52242
                     /locus_tag="VBC52_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881675.1"
                     /translation="MVEIRINGELVTFDGNFRDAFIFTIDYLRDSEEPTLRQTYNEFK
                     DYTDEDLMEYIETEFDVKPELIVNRRLDSKWAFKSHILED"
     gene            52245..52406
                     /locus_tag="VBC52_000064"
     CDS             52245..52406
                     /locus_tag="VBC52_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881676.1"
                     /translation="MSEELYESTRYWQSRYSDLMSDYLKEAEENIELKKQLKRLKAEN
                     WQLKHRKRK"
     gene            52407..52889
                     /locus_tag="VBC52_000065"
     CDS             52407..52889
                     /locus_tag="VBC52_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HEO6881677.1"
                     /translation="MAYLYELQGIYAQLSAMDLDDETFQDTLDSIDFQSDLENNIEYF
                     VKMLKNTQADIEMYKNEKETFYKKQKQAEAKAEKYKDTIRLAMDLSQKKKVDAGMFKV
                     SLRKSKKVEVLDETKIPFEYMQEKVEYKPKKDEISKVLKSGIDISGVQLIETESLQVK
                     "
     gene            52890..53564
                     /locus_tag="VBC52_000066"
     CDS             52890..53564
                     /locus_tag="VBC52_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HEO6881678.1"
                     /translation="MSMTFAELQTKMQITKTTKQGVKYTFRNAEDIFTHFKTLNSGWE
                     LTVSDELVELIGRIFIKATATARLGDEQHQATRYAELDSVPVLNTKDYKTGEPKQIQQ
                     MQVPQWTGAVSSYAGKYALQGLFGIGEEDVDAIVTEDTQRKEQKTSRPTTSKTPKISN
                     IQVETYKSDLNDIAKATNQNVEELTKWLTDTLKVGTLENLHTEHIVSADELINKLKKK
                     AGLKND"
     gene            53557..53973
                     /gene="ssb"
                     /locus_tag="VBC52_000067"
     CDS             53557..53973
                     /gene="ssb"
                     /locus_tag="VBC52_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO6881679.1"
                     /translation="MINNIVLVGRMTKDAELRHTPSQVAVATFTLAVNRRFKEQNGER
                     ETDFINCVIWRQSAENLANWAKKGTLIGITGHIQTRNYENQQGQRIYVTEVVAENFQL
                     LESRNSQQQTNQSGNSSNSHFGNANKMDISDDDLPF"
     gene            53976..54176
                     /locus_tag="VBC52_000068"
     CDS             53976..54176
                     /locus_tag="VBC52_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881680.1"
                     /translation="MTKPRKQRIYAIYDDDKFVDVGTKEELSARLGIKKATIEQYMTK
                     SYQARPSSKRIAIFVGVEEIEF"
     gene            54166..54654
                     /locus_tag="VBC52_000069"
     CDS             54166..54654
                     /locus_tag="VBC52_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001009815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEO6881681.1"
                     /translation="MNFKTEFEIPVEPKPQTRPKFSKWGTYEDPKMKKWRKQVTGWIE
                     KNYNGPFFDNCVKVDVTFYMRAPQTLIKEPTARSKSKTVQIYQKFINELIWHVKKPDI
                     DNLVKAVFDSISDAGYDKIQKSGIVWSDDNIVCDLRATKKYSPNPRIKVKIEEIDERT
                     NG"
     gene            54635..54979
                     /locus_tag="VBC52_000070"
     CDS             54635..54979
                     /locus_tag="VBC52_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881682.1"
                     /translation="MNELTDKFYSIFDSSILRRVKELNLDDKTSERLRLNISNNKRRN
                     ILPRPYVIEAFKDYFDKDTYVQLYLKSYREYHNPNSHETDIFIKLNKKHRDTKLDHYK
                     KVKRLMYAAMTF"
     gene            54991..55287
                     /locus_tag="VBC52_000071"
     CDS             54991..55287
                     /locus_tag="VBC52_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HEO6881683.1"
                     /translation="MADKINAESMQAAYNENYQMFLAKNADYGNSFEKSLNDFGFIAG
                     VVRIGDKYNRLYNLISSDKNVSESLSDTLNDMANYCTMLSIWLEKTENANDTRS"
     gene            55271..55510
                     /locus_tag="VBC52_000072"
     CDS             55271..55510
                     /locus_tag="VBC52_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881684.1"
                     /translation="MTHVVRVYDHIGGRVLPITRRVRRMTKYIEIKDDWKKAVDHLND
                     FIEKYSYKKIVVVGYQVVRNPETNGTCTHVLVKVD"
     gene            55541..55705
                     /locus_tag="VBC52_000073"
     CDS             55541..55705
                     /locus_tag="VBC52_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881685.1"
                     /translation="MTKEEVIAFLTEQRDLRLVAYEWGKDNLSVFARWQLEQANMYLD
                     IIEWIEEVTE"
     gene            55702..55971
                     /locus_tag="VBC52_000074"
     CDS             55702..55971
                     /locus_tag="VBC52_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881686.1"
                     /translation="MTEQQMIDCLLYELAKKDKLNIRRNNIITFLSIVLMAISILNVA
                     LQDHYKLQITGLRTQLSRTQKQLKRASEQNQRQTKRIAELTGNGG"
     gene            55975..56118
                     /locus_tag="VBC52_000075"
     CDS             55975..56118
                     /locus_tag="VBC52_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881687.1"
                     /translation="MIDEILKRLNKEFDNDLDNYEQERYAGYMDAIGVAIEIVEEVKR
                     GRK"
     gene            56115..56627
                     /locus_tag="VBC52_000076"
     CDS             56115..56627
                     /locus_tag="VBC52_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEO6881688.1"
                     /translation="MNIEEAKKLIDKQSIGKGGVGDIPVVKTHIVKVLLDQLDKPKLE
                     VPQMIADVIESFDEDVNYLHEHMSYQSDEVREWLTHNEREFYEAWLAYPNITVEKENL
                     YTVEIPNPNERQLSFVLMRQLSGNVSIKVMHRDNLDLLKIDNNLQLTESEIRKDFDWA
                     WQFRKDVENE"
     gene            56648..56947
                     /locus_tag="VBC52_000077"
     CDS             56648..56947
                     /locus_tag="VBC52_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881689.1"
                     /translation="MNYDKNKNDAKKNFIIALVLLPFGLVLSGFVIKYGWNNILSTID
                     GVPSINLPQAVGINVLISPFASKKNTDEDFATVIARAFISPLVVLLLLWIVTLFM"
     gene            57135..57329
                     /locus_tag="VBC52_000078"
     CDS             57135..57329
                     /locus_tag="VBC52_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017827938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881690.1"
                     /translation="MTYLKKLFEAVKFLNSMEKQNKKIMITFHNKKVHFYELEEITES
                     SSGTVEAKAKMLSLWGKIKL"
     gene            57326..57592
                     /locus_tag="VBC52_000079"
     CDS             57326..57592
                     /locus_tag="VBC52_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881691.1"
                     /translation="MKEKTIFISKKYANDFNNDKYNLSSGYYFRSGEKHDIAIVKYGE
                     KDYLKNTDLAYVVCDKIVDADSISFVYHGEYETWHFKLLNTEAN"
     gene            57983..58417
                     /locus_tag="VBC52_000080"
     CDS             57983..58417
                     /locus_tag="VBC52_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEO6881692.1"
                     /translation="MTFFPEINIQKTKSNAKRKLREYPRWRRIANDVDTQKVTATYSF
                     EPRQSHGTPSKPVERLALNRVSAEQELEAIEQSVSMILEPEKRRILYDKYLSPYKNAD
                     KVIYTELCMSESFYYDTLDAALLAFAELYREGSLIVEQGVFD"
     gene            58583..58661
                     /locus_tag="VBC52_000081"
     tRNA            58583..58661
                     /locus_tag="VBC52_000081"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            59080..59988
                     /locus_tag="VBC52_000082"
     CDS             59080..59988
                     /locus_tag="VBC52_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEO6881693.1"
                     /translation="MLKLDEKKIRKGKPFGLPYQGSKKKISKKIVEIIKQNFGTDKKI
                     YDIFGGGGAITAECMLNNLEVHYNDLNNDVTDMFKRVITQDREWLKTLIISREEFLKI
                     KTKTNKTVDDNIKLLVNSFGNNSISFLYNIELSDIKYKLAVEIINKHDVFSGYKQTDT
                     YKETCLLCEEEKLGKIGNLQRLQQLEQLQRLQQLERLQQLEQLERLEATNLDYKAFSN
                     VDGAIFYLDPPYEMTSQHSYIGDTFDSQLFYDWAYEMSKENIVIVSSYEISDDRFKCV
                     YEFKTARSTMQGGSACGKTEKLFMVV"
     gene            complement(60041..60424)
                     /locus_tag="VBC52_000083"
     CDS             complement(60041..60424)
                     /locus_tag="VBC52_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEO6881694.1"
                     /translation="MLIYPAVFTHDNETNSIGVRFPDVPEAITFGNDLKHAYEMAVEV
                     LGFALEDYTDYPKASSVSDLKEQYPDSDIALIGIDMIAYMKKYHSKKVRKNVTIPEWL
                     NNAAEDKNLNFSQVLTEALELKLQA"
     gene            complement(60468..60656)
                     /locus_tag="VBC52_000084"
     CDS             complement(60468..60656)
                     /locus_tag="VBC52_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEO6881695.1"
                     /translation="MPRTAREIIKLLKKNGFVKVSQNGSHAKYKNFETGNITIVPMHK
                     GDMPKGTEDDILKKAGLK"
     gene            60765..61262
                     /locus_tag="VBC52_000085"
     CDS             60765..61262
                     /locus_tag="VBC52_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEO6881696.1"
                     /translation="MGKLTLKQQKFIDEYIICGNATDAAIKAGYSKKTAGQIGEQNLK
                     KLEIKQAIQSRMKVLEKSSIATADEVLRVFTQILRQELTEEVTELNQITGEFVTIEKQ
                     PSIAEVIKAGSELMKRYPTNLELKKINLEIEKLKSQIGGDEGQDEKIANFLNMVKGAI
                     SDGVE"
     gene            61249..62490
                     /locus_tag="VBC52_000086"
     CDS             61249..62490
                     /locus_tag="VBC52_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEO6881697.1"
                     /translation="MELSKLYTRKQLQVLDYIWNNDWFICGLHGAKRAGKTVVNNDTF
                     VTELYRVRKIADDLKIDEPIYILAGTSSTSIQNNVLQELYNKYGFEPKYDKHGSFTFC
                     GVKVVQVYTGSISGLKRARGFTSFGAYVNEASLANETVFKEIISRCSGEGARIVWDSN
                     PDNPNHWLKTDYIDSDDDMIIDFSFKLDDNTFLSKRYIESIKSATPSGKFYDRDILGK
                     WTVAEGAIYSDYDKNVHEVEELPKMTRFFAGVDWGYDHYGSIVIIGEDNNGNYYLVDG
                     IAEQYKEIDWWVTRAKEFIQIYGNVTFWADSARPEHVARFKRERIKARNGRKEVVAGI
                     EYIAKSFKDKRLFIKRGCIPRFFDEIYQYKWKPNSTKDEPQKEYDDVLDAIRYALYSQ
                     HKEDSISKTNNFNVLYQGLKN"
     gene            62503..63996
                     /locus_tag="VBC52_000087"
     CDS             62503..63996
                     /locus_tag="VBC52_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO6881698.1"
                     /translation="MAHIEDFIDSTGEHKLLELRFHRESRMRYQISDISALFDDNYKI
                     LLEYLKHHENIQKPRIQELLDYAEGNNHEVSKSGRRQDEDMADVRAIHNYGKYISTFK
                     QGYLVGNPIRVEYDDEVNNELLKELAKKNNFHQLNRQLIKDLSKVGRAYELAYRSADD
                     KTKVIRLDPRETFIIYKNDVDKDSLIGVRYYNKSQIDKTDKTVEVYTSSEVIFFEFNG
                     ELTETDRQPHAFGAVPITEYLNTDDGLGDYETELSLIDLYDSAQSDTANYMQDLSDAI
                     LAIIGRVSFPEYVDTQEKAITYLRAMRKARLLNLEPPVDSEGREGSVDAKYLYKKYDV
                     QGTEAYKNRIAEDIHRFTNTPDMTDNKFAGNQSGEALKWKVFGLDQERVDLQALFEQS
                     LKRRYKLIAHVSEFLSEIKDFDISKLKIIFTPNLPKSDQEKINDFKALGGELSNKTKM
                     FVTGIVDDPDEEEDKIMQESQSGSLLAQKLEAQTRMSDKELAHGHAH"
     gene            63980..64951
                     /locus_tag="VBC52_000088"
     CDS             63980..64951
                     /locus_tag="VBC52_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEO6881699.1"
                     /translation="MDMHTKEGKSYWRKRVKKEMEAKDKKDVSLGKSMKQIHDYHFRE
                     IEKEIESFYQRYADKEAIDLKLAKKAVSDVDINAYQKKAKELVARANEMRKEGIKVTK
                     ANFTHQENIDMAVYNLKMKVNALELLQLNIDLAMQNLSEDEYKATKRFLEDGFEEELK
                     FQSGLLGTSVSSQNDIKNLAKATINANFKGATWSENIWQRQDDLRKIVKEEVYKAVTK
                     GDNAIKLSNKLKKEFEVSDSYARRLAITEHARVQMEVSKISIEDNGFNGFEILPEPSA
                     CSICKGIASDGPYPMEKWDTGNTAPPFHPHCRCAVVGEDIEHKKGKK"
     gene            64948..65151
                     /locus_tag="VBC52_000089"
     CDS             64948..65151
                     /locus_tag="VBC52_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881700.1"
                     /translation="MNKRIKKKRKLEYYIASLVAENVMFSKELIKQHERIEQLEKIVE
                     HNAQATNNELSRIKKHSKKKWKK"
     gene            65262..65840
                     /locus_tag="VBC52_000090"
     CDS             65262..65840
                     /locus_tag="VBC52_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEO6881701.1"
                     /translation="MADLVNNGVVDEVTQEEVETQEEVKAEATSEKTFTQAEVTEMIQ
                     QNVNRAVAKAHKDAQEQFKAEQDEAKKLAKMNSEEKANYETQKLLEELQQLKDDKTRN
                     ELTAVARKMFSEADINVDDDVLSLAVTLDAEQTKANVTKLASAFAKAIADDRKSLARQ
                     ATPSIGSGSITTQSNFGASLAERAGKVNTKLF"
     gene            65850..66233
                     /locus_tag="VBC52_000091"
     CDS             65850..66233
                     /locus_tag="VBC52_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HEO6881702.1"
                     /translation="MNKRTVKTSKEILHNLPYEAISVTLDANAFGKGLASAGTVLAGD
                     GASVFKDRTKKVKPVRNAEVSGADHIDGILLTDVDLSKGDATGALVYRGTVNSDKLVD
                     QELAKDTSTLATKLPHIVFVKGGTK"
     gene            66233..67285
                     /locus_tag="VBC52_000092"
     CDS             66233..67285
                     /locus_tag="VBC52_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HEO6881703.1"
                     /translation="MALIYDVVTSANIKGFYDKQQANVDLTLGEKAFPSKQQLGLKLS
                     FIKGAAGKPVSIKAAAFDTKVPLRDRIAVVLLDEEMPYFKEGMLVKEADRQQLNVLAQ
                     TKNQELIDTVLSTIFNDETTLIAGAKARLEAMRMEVLSSGKIHINSNGVMKDIDYGLT
                     GTQTTKSEQKWSEKDTANPLADIEKAIETVTERGHVPEAIVLNSKTFGYIKNAKATVK
                     VIKPLAPEGSIVTKAELKSYLSEELGLNILLKDGVFVNDAGESKKYFPDGVVTLVPNG
                     NLGYTVFGTTPEQSDLMGGQATDAQVSLVETGIAVTTTKTTDPVNVQTKVSMIALPSF
                     ERLDEVQIVTSSEVSL"
     gene            67298..67552
                     /locus_tag="VBC52_000093"
     CDS             67298..67552
                     /locus_tag="VBC52_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881704.1"
                     /translation="MALVIEAFRDKETGFIYKVGEEYNGARVEFLTGKGVLKATDTQS
                     TNFSNLKVDELKRELDELGVNYDSKAKKAELLELLESHTD"
     gene            67568..67921
                     /locus_tag="VBC52_000094"
     CDS             67568..67921
                     /locus_tag="VBC52_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEO6881705.1"
                     /translation="MEKINTQTIINNVKLDLDINDTLQDKLLEMLLKRITDHFSAEYG
                     TNEIDSAFSFVLEDCLIARYNRRGAERAKSESVEGRAITYYDFLNEFEPYDLMIKSKL
                     NISNQKSKKGGLYFL"
     gene            67918..68217
                     /locus_tag="VBC52_000095"
     CDS             67918..68217
                     /locus_tag="VBC52_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881706.1"
                     /translation="MRYNDRVTLLIKANGEPRYDPTLGKRVGGEVKKDVVPCNISEQG
                     IELSNLLDEKLDLDRRIVRLRHSVKKVDRVLIKDKAYRVVTSRNKALYVEEIVND"
     gene            68210..68590
                     /locus_tag="VBC52_000096"
     CDS             68210..68590
                     /locus_tag="VBC52_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017827930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881707.1"
                     /translation="MINLTLEGEHELLSALKKEVTFENKRKAVRKHGTKMHSKAIDKA
                     VFNGHYEWQKGKGRVFVKPTGATRRSIKLEFSNQSTVAKVKAGTNYSGYLETGTRLME
                     EQPFMKPALDSVIDNFIKDLARVE"
     gene            68587..68976
                     /locus_tag="VBC52_000097"
     CDS             68587..68976
                     /locus_tag="VBC52_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HEO6881708.1"
                     /translation="MMKQPDQQLFDEIFKRLTDIGLTVYDFLPPLGTKYPFVVMGDTH
                     IMPRATKTQLIGKCSTTINVWGDGMDRKLISDIVARIMQAVSEINQIENRRWSMIIDD
                     SDTEILKDNSTNENLYRGILNMYFSFI"
     gene            68985..69512
                     /locus_tag="VBC52_000098"
     CDS             68985..69512
                     /locus_tag="VBC52_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEO6881709.1"
                     /translation="MVTSSPVYGKDNILMFRVLGEKKAAAKLSFQTEHKWKFSRKTDA
                     KITKDGAINSDKGLEVTLEIKGVASRDELNTTLKNAVLDGKQIEVWDIDLNSNNNSDN
                     KYDAEYAIGRLGSWEVPSNVEELSEISTEMAIDGKPVKGKATLTKEQIKAIQYVFKDV
                     TETNGDTVSHVEASS"
     gene            69587..69940
                     /locus_tag="VBC52_000099"
     CDS             69587..69940
                     /locus_tag="VBC52_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881710.1"
                     /translation="MKEITIAGKTYPLNFGFDFIREMDKRHFVENNGFKFGTGIQTAT
                     LQLSIKNPLILEDIILSATHTLNSIPSKEEIEKWAIKQAEDNKLEEVFEGFLTSLKKA
                     PLSKAQVKQLLKSMN"
     gene            70060..70335
                     /locus_tag="VBC52_000100"
     CDS             70060..70335
                     /locus_tag="VBC52_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881711.1"
                     /translation="MTVREYNLRMKGYLIKHLETERNIYLSAFIKRNAEATNQSGEYI
                     YKDFKDFYDYDERKRKLLGDKPKQLDKTIVNRAKRVKELREKGGINV"
     gene            70328..72976
                     /locus_tag="VBC52_000101"
     CDS             70328..72976
                     /locus_tag="VBC52_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEO6881712.1"
                     /translation="MSNRSYTVQAILKATDTNFTSTMNKVQSAAQATIDKIKSIKDSN
                     ISTLGKIGEYTTMVGQGMQSVGRSLSKYVTLPIVGLGVAAAKTFGDFESQMNRVKAIS
                     GATGADFEKLRKQAIDLGASSVFSAKEVAQGQEMMASAGFNANQILAASPGVMSLAAA
                     SGGDLALASEAAATAVNMFSLNASEATHVADVFAKAAADTNSEVADMAEALKYAGPVA
                     GAMGISMEETAAAIGIMSNAGIKGSQAGTTLRTAITRLAKPTDQMQAVIDGLGLSFFD
                     TNGKMRSLTEITGQLREKMSGLTDQQKSAALSILFGKESLSGMLALINATPGELSKLT
                     EGLKNSKGAADKMANTMNSGLKGTIEQLKGSLETAGITIGGILNPLLQGVIGKIQAVI
                     DWFNKLSPAGQKLAVIIGGIGAALGPLLVIFGTVIIFIGQVTASLAGVSTAFTAIAGI
                     FSIISGPVILVVAAIGAFIAALVVAWNTSETFRNTIISGWKSMVDAVTPLIETLKQLI
                     LSIFTFIQANSATFIGGLKLAWSALVEALSGLFLVITGVVQVGMSVINTTIKVILAII
                     NGDWNAAWTAIKSGVGAIWEGIKNIIIGGLTFLVSLFVSMLGVFVSVFSAGWSAIKGI
                     FSVTLKFLVNVVAAGMSAIGNEISSKINTAKSVAILAFNAMKNGISTAINAAKEVVSS
                     VVNRIKNILNSLANINLGAAGRAIMDGFLNGLTSAFEKVKNFVGGIAGWIQEHKGPIS
                     YDRTLLIPAGRAIMGGFNKSLTDSFEPVKKNVSSMASRISEEFQNGLNKLKDINISEI
                     TEGLQSSFDVTHYASFGGLNDSARKLEDDRFVALKDAVLAIRDFANRDVVVTVNGKEL
                     ARTAGDNFTEYQKQKEIMNNRMKGLI"
     gene            72977..74539
                     /locus_tag="VBC52_000102"
     CDS             72977..74539
                     /locus_tag="VBC52_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEO6881713.1"
                     /translation="MANFSYKGVDLSPFLNCLNIVRTIGNNRSIATRKINELGEAIQS
                     VSFGAKTIFVTVSFKTKEIGASKFVDTTEPSTYSYENLNKLREKIAGILHSKTTFKLT
                     LPDEPDRYYMAVPKGDIDLKGISDWYDETVIEFYIPDGVAHSTTYKKFSDYTQEGNKL
                     IFNLQNDGNTNAYPIIKIKHNSENGYIGIANETGAFALGSSEEEDGTIVHRNESLFDY
                     SKAIAQALEGAPNVAKLNYMPPTFDSELKRMRLDNILGSGKGGEYVAIGARGTTPGYG
                     EHVGTRTFIINPDSNGEYTLNEHLWWQQIFIATAQDQKGFLKLCVTGENDEFLYGVET
                     YKRKNGFETEYNFFALDDDGVGWRFYKQFTFLADRNYHNPFSMNRSRAVEIFREEDKF
                     RIYFNGAHHHVTVPSLKGKKSRKIHLAMGTCSDSSKYINYNLFEKVNFEKMGVSHYNN
                     IVNKYQPGDEVVINFENDTVKTKDLDSIQDMVLGSQLISIPPGKTELVMQVSKFSQSA
                     PNVEILMEERWL"
     gene            74530..78357
                     /locus_tag="VBC52_000103"
     CDS             74530..78357
                     /locus_tag="VBC52_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6881714.1"
                     /translation="MVIITLVIHDSKLHPVLLLDNDKQGALNYYDDLWTRQLTTGSSA
                     FEFSVYKKTLLGDNPLNHKYHALNDQAFVSFVHKGKVQLFNIMQVEETETKIHCYCEN
                     LNLELLNEYCNAYKATKAMSFEEYLVQFDILNWGALTIGTNEVKDKKLTLEWTGQDTK
                     LARLLSIANNFDAEVEFETQLHNNHTFKAFIVNVYKEYEEGVSYGVGRDRSDIVLRYQ
                     KNVTGITKKLDKRQIYNAIRPYGKKTVKGERVISNPVTRKVTKTVGSNRTYLGGDLKY
                     YGHTIKKANVQAIINYAVQYNILPSGIITQLYLESFWGDSTVGKRDNNWAGMSGGAQT
                     RPSGVKVTTGMARPANEGGTYMHYASVDDFLKDYTYLLAKQGIYNVVGKKNIADYTKG
                     LFRAGGAKYDYAAAGYQSYTNLMTNIRNGINKVTGNILNTIDKLWQTPVQPITAVNVA
                     RRATKTMQALNEATRLKGRRIGSGQCYALSGWYAKKLDGAWIDSSVGGIRGRIGGGMA
                     AALIGTDYNWGAYGWKLDRSPNAGNLQAGGIYNVKANFGAPFYTTQWGHTGIIKSVSK
                     TRVTVLEQNYAGRMYVMENSYEINAFARGLQTVCYPREIAQGMSVNGATTQQVTGGTQ
                     ISYEEVVQEAQTETYEEEQIIYIDNSIYKEWKDENGKVEYYLKNGFLYAPLSRDRYPS
                     VLTGNETRDNWIRKDMEVETDSQEVLMSTGLKDLKAHAYPAITYEVDGYVDLELGDVV
                     RIQDDGYEPPLILTARVIEQEISITNPSSNKTKFSNFVEKESQLASDLISDMLRLYDE
                     SIPYDIQLATSNGVAFKNGVGESVLTPNLQKNGKDYDAIYFYKNGDSLIEIGPSLTVK
                     ASDFNHVLNITVEAYVNEELVASTQISFTDTEDGEKGDDGATSWTAWANSKDGKVDFS
                     ITEAKNRRFIGTYTGLTQSTNYLDYKWIDMSANVVIGTQNLLDGTKSFSGSWFTEGTI
                     FETTKISEYPFEFKKWKSGNKVSHTIEFDVKAGVTYTFTAAIARENAGRLYFYLYDLF
                     ANHITSNTPRETIIENVTTDIQMFKVTFVPLRDGKIKPRFAMLASDAGWFMTGGYMLV
                     KGNKSGDWQESEADKASNLDSKADQELTRAQILALEERTAIARENAIAEAMQHTLSEV
                     ETKWKLWYDLNTIDEKQKVANDIAQLFDRTTEFKQLLGEASAKFSFINNETLIGEEGI
                     AIGDKDGKAKLFLSNDSISFVTNGVAQMTLTGDTLTIKNGLFTERIQIGNFVEEVYDR
                     NPLFNVIRAIRNS"
     gene            78368..80371
                     /locus_tag="VBC52_000104"
     CDS             78368..80371
                     /locus_tag="VBC52_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881715.1"
                     /translation="MGTSTFSGTWGNNLTLDVRTSYTQNLVGNYSTVTVKVYEKISSY
                     GYIDYPGERTMTIVVDGKSYNESVNVDINYGQTKELGTYSYRINHNSYGSKPKFNVTV
                     TLPINFSNYGSASVTNSVSLPTIKRASTATIASGNIGSAVAITIKRQSDTFRHTLKFD
                     FKGLTGTIASLVDTSYSWTLPASLSVLIPNDRSGTGTLIVETFTSDSQKIGENKYTFT
                     ATVPDIAAYKPILASISLSDANTLTGSLITGNNYVRNMSKLKVSFGNSAGANGSTISS
                     YNAEIVEKGKAIFGNGSVFDMLDFVGTATIRATVTDSRGLTSEPVDTKINVIDYFLPI
                     VTSAKVVRSQQNPDILQVLPFVKIAPIIVGGIQKNQLKMSVSVAPYNTGIYAVDSGAA
                     TNTWSTISQMSGAPLNLGGTYDKSKSWLVKISVSDNLMSATPIIQPVASEFVLVTKAP
                     SGVAFGKIWEHGIIDAKGDVYVDGNIYCGDKPIQQKQLALNNGGSFRHDDTDLNSLQD
                     TGFYCVFRGANRPSGAGPGYLTVVRHETANYAYQQFYDRTNKTIFTRVLENGVWSGWS
                     EYVKKDSSQTTGWITIGNGFSYRQTSSTVTVKYNFATNGIDKLTVGSMPTNLIPSDMM
                     FAVTAWTIQLNVLNVQVSADGRILWFNPSKWTVNVKGQIQWTI"
     gene            80385..80711
                     /locus_tag="VBC52_000105"
     CDS             80385..80711
                     /locus_tag="VBC52_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEO6881716.1"
                     /translation="MEILNKYPVMLEDKSIAKVNAIVAVDLPHVRGNLTFDLPVDFDN
                     KSFAETLEKCEQIFYDEKYKDKAQSEKMTELSTSTSTGTQTLINLISTLYAKEVLKDE
                     DLIAIG"
     gene            80686..80898
                     /locus_tag="VBC52_000106"
     CDS             80686..80898
                     /locus_tag="VBC52_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881717.1"
                     /translation="MKILLLLVRIFLQEEGIDMMIKLFAIDLYYGRMAWSSFVKKGFS
                     EFINNKTKEQLAIMCDEELLAEILAS"
     gene            80911..81213
                     /locus_tag="VBC52_000107"
     CDS             80911..81213
                     /locus_tag="VBC52_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881718.1"
                     /translation="MTVEQAERIAQSQFVWAILFILLFMIVVGYLVRTSDKREKKLMD
                     FHDQSKSESNKREEWLKGHLDKNTEQLQDISQTIGVVQKEMSYMSDRIGRLEKEEK"
     gene            81215..81550
                     /locus_tag="VBC52_000108"
     CDS             81215..81550
                     /locus_tag="VBC52_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO6881719.1"
                     /translation="MNEFTTQIITASVPIFGIIAGILTHEVKKLLIKKGGEKAVKIAE
                     IVARNAVEAVEQISVEVGIKGQDKLTEAKTAVIDGLSQYNINLTQTQLETFIEAAVKR
                     MNDEWKKGQ"
     gene            81552..82271
                     /locus_tag="VBC52_000109"
     CDS             81552..82271
                     /locus_tag="VBC52_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6881720.1"
                     /translation="MVINIEQAIAWMASRKGKVTYSMDYRNGPSSYDCSSSVYFALRS
                     AGASDNGWAVNTEYEHDWLIKNGYVLIAENTNWNAQRGDIFIWGKRGASAGAFGHTGM
                     FVDPDNIIHCNYGYNSITVNNHDEIWGYNGQPYVYAYRYSGKQSNAKVDNKSVVSKFE
                     KELDVNTPLSNSNMPYYEATISEDYYVESKPDVNSTDKELLVAGTRVRVYEKVKGWAR
                     IGAPQSNQWVEDAYLIDATDM"
BASE COUNT        27930 a        13028 c        16511 g        25020 t
ORIGIN      
        1 gtttagaatt tatttggagg atttgttaac atggctaaca aacaagattt aattgcaaaa
       61 gtagcagaag ctacagaatt aactaaaaaa gattcagcag cagcagtaga tgcagttttt
      121 gcagctgtag cagattacct tgctgaaggt gaaaaagtac aattaatcgg ttttggtaac
      181 tttgaagttc gtgaacgtgc agctcgtaaa ggtcgtaacc cacaaactgg tgcagaaatc
      241 gaaattgcag catcaaaagt tccagcattt aaagctggta aagctcttaa agacgctgtt
      301 aaataattcg tctagaaaaa ccttgtcata tcaatgttta ttgatagcga caaggttctt
      361 ttttatatcc aatactcaat aaatgactag agagattaaa ggaatttaga gtttcaaatg
      421 tgttagagca tacccttgaa ttatcttaac tgtgatgtta taattcctaa aagataagtt
      481 aaaagagagg ttgcgaaaga gaatgtcaga aaaagctata ctattaatta tccttattgc
      541 ttttgttatt atcgctatta taaagatagc tctgttaaag ttatatatga atacgataaa
      601 acctaaagta gataatgaag gaaacatcat aacaaagcct gtaaaggatg atttaacacc
      661 cgaggaacgt gaagaacgtg aatttgataa agattggtaa aatcaatata aaagagcaaa
      721 caaggtttgc tcttttatta tttttaaagg taattaagtt gtcctcgaaa atcctccaat
      781 gattgatagc ctttatcagc cattatttct tttaattctc tgcttacacg ctgaaaaatt
      841 tctggtcctt ctttttgtaa agcggttccg atttggacca tactagctcc acatagaatg
      901 tgttcaaagg catcacggcc gtttttaaca ccacctgttc cgataatttt aattgaagga
      961 tttaaacgtt tgtagaaagc atggacgtta gctaaggcgg taggcttgat aaaatcccca
     1021 ccgatacctc caaagccatt tttaggcttt ataacgacag tttcatcatc aataacaagt
     1081 ccatttccga tagaattaac acagttaata aaagctaaag gatatttgtt aaaaattgta
     1141 gctgcttgat caaagtgaac gatatcaaag taaggtggta atttgatgcc caatggcttt
     1201 tgataatagc taaagatttc tgaaaggatg aggtcagtca tttcaaaatc ataagcaatt
     1261 tgaggttttc caggaacatt tgggcacgat aaatttaact ctattaggcc attgtaatta
     1321 ctgttttcaa cctttttaag aataatatgt gtctcttctg gtgataaacc tactaatgat
     1381 aaaacgtgat ttttgctatt gtcttgatct tgtaattcag tcacgaaatc aagataatag
     1441 tcaatacctt tattagggag tcccatagaa ttaattgatc cccagtcagt atctgcataa
     1501 cgtggttgag ggtttccctc tctagcttca agggtacctg tttttgtaac aaaggaacca
     1561 gcttcagaat tttcaatagc taataactct tccttagtca tacaataaat acccgctgca
     1621 ttcatcaaac aattgtcaaa actaaaacca gcaatttccg tttttagcga taccataata
     1681 tcctcctgaa gataaatttt tactatttta tcacttgatt aatagagctg caagaaaata
     1741 atgataatgg taataaaatt tcgctaaata acgaacatta ttgtttgtat aaacaataat
     1801 agtaaagaaa taaatgatgc tttctataat gcataaaaaa taagcttatg taagcttatt
     1861 ttttatgttc ggcgtaagat tttctttagt aattgaatac tcttatattt taatggattt
     1921 ggatagtagc ggaaagtacc cattttacga acaatgtaac cattaaaatt ctgtttaaag
     1981 cgaagtacgc catctgaacc atcaaaattt ccttgaatac ctaaaaaatt atagaaagtt
     2041 gattgacgct ttaaagcctc ttgcataaca tattcttgaa gaactgcagg cgcataaaat
     2101 ttattaaatt ctgtgtatga gccagagaaa agatagacag tttctttcgg gctataaata
     2161 aagaggctac cagctaaaac aacatcttga tttccaaatt tttgaataaa aggttcgact
     2221 tctttgcgac gtttatctaa actagctagt tgattgacaa gctcagtacg ttgacgatgg
     2281 tatttggcag aatctgtatt attttgatgg tgattatcaa ggactgtaat ctgttcttcc
     2341 aatttctttg catttaattg taggttatga tcatattccc taaagtttag agttgcaatg
     2401 acaaattccg ccttatctcc gaaactatca taaaaatctt gataataatc tagagattta
     2461 tccatatagt cccggcgatc agaagtagaa gaagtaatat ccttaaagat atgtagttct
     2521 tcccgcttga ggacacgtat ttttatacca aaagacatgg ctttcttaac caaggggcgt
     2581 ccttttttag agaaagattt aagaagattt tgaggagtga taccttctag atttttaaca
     2641 taatgccaat caggttcacc gccaggataa ccaatatgaa gcccatcgtg gtgataacct
     2701 atactagtca ggtcatcaat aagataggtg ttcggtgcac ctttaggctt accttcacca
     2761 gtaaactctt ggtaggtatc ataaggttta attaataatt ctaaagcgcc ctgagatttg
     2821 gcatagttct gtagttcttt ataaaaatgt ttcagatatt tagaattgga atgggctgga
     2881 ccagaattga cttccatatg aagtccacca gtcattggga tggtataaac aatactgatg
     2941 atttccattt tcccatcgat ttggtagccc atgtaggtaa tgtcataccc acgttttttt
     3001 agaagctttg ccatctcagg ggtttgcata aacgattgta aatcgtaatt tcctgaataa
     3061 ctttcgaact ctttagctgt taattcttta agcgccataa aagtatcctt tcctaactag
     3121 ctattacgtt ttttgcgtaa ggcatagaca taattagagg ctttataaag caatctgttc
     3181 actggaatgt taaattctcc aataaattcc tcaatgattg gttcgaactt agacttaaaa
     3241 tgatataaac caccagataa gtcattctca atccccccca tattttgcca aatatttcca
     3301 cgttcaaaag ccctttgagc tgtttcaaac caagtgtaaa taggagcaga ataagattta
     3361 aagtaatcgt ccataccagc ataaatattt tctgatgtat taccaaattc tagagtgagg
     3421 gttgcagcca atggaataat gtctcggtca taggcttttt gagcttcttt gagaaaatca
     3481 atttctttat ttaatcgatc aatcgtacct tggtgttgtt taactttttt ctcacgatta
     3541 agttcaagtg attttattct ttcagattgg gctatctgac attctttttc tattttttct
     3601 aatcgttttg caacatctag cgaagccatt gtgatgtatg agttattagg atacgtatca
     3661 aggagttttt ggtagtagtc aattcctcta agatttatcc ctttacggtc ttccgttttt
     3721 ttcatcaact ctgcaaaatc ttcaagtagt tcatggctac caaattgtat atctactccc
     3781 ttatttttgg aagttcgaat agcttgacga gtttttttag acattttatc aagtccaaat
     3841 tgattagcat ataggtttgc ctgaaaacgt ggttggatag tatgagctaa atctgttgtc
     3901 cgaccagtcc aatcagcacc tgcacgctgt aaatgatcaa tggcgattaa cccttctttt
     3961 tcatcgggcg aattttcaaa atctttggca ttgaccattt ttaaatagat gagaggatca
     4021 cacttgataa agagagctct ttgttttttg ccaaaagctt taaggtcctt aaggacctta
     4081 gttacaatat ctagattgga gtaatccatg attggtccac gcggaatata tagcatggag
     4141 aagccaagag gaagtgattt gatgagaata ctagcgctgg caatttgggt ttcaccgtca
     4201 aaaaagccaa gtaattgact accccagttg tcttttactt tgggccaatt agaagattga
     4261 agtaggtttg tttggttgga ttcgctcaca aagcgatcgt gttcgacagc ggtaatttct
     4321 ctatacatga gtttcctttc ttagtgagag tgtctttgtt tttttcgaat agtataggct
     4381 aggttggcca atttatataa taatggattg attgctagat taaactcgcc aatatattct
     4441 tcaaacattg gattgaagtt acttttaaat tttgttaaac catcgttaag acttccctcg
     4501 acaccgccta gattagcaga tacaatatcg tttttgaaag catcttcgaa caccctagca
     4561 ttcaagagat actgtggata aaatttacgg aagctatcat ccataccagc atacaacatc
     4621 tccaggacat tgccaaaacg aatggacaaa attcctgcaa tgacaggctc atctggatat
     4681 ttcttgctaa actcctgaaa ttcaaggata tattgtttaa cagaagcttc ttgttgattt
     4741 agccttgtca ggcgactttt ttgatgggat ggagtttctg ataaatcttt ttggatttga
     4801 agaagttgtt ctttaaattg cgctaatctt ttaggaagat tgactttagc taaatagaga
     4861 taggcatctt ctccataaat tgtcattagt tgatggaaat agttttcatt acgtaaagct
     4921 acacctttac gattctcggt taatgctaca accgtggcga atttaggtaa atcatcaatg
     4981 ttggcacgat aaatttgaac accgcgatgc tttgcatctt ttatcagacg tttagtgtgt
     5041 tttggaaagt tttcttctat atttgctttg gtatagatat tcgcttgaaa acgaggttgg
     5101 atggtttcgg aaatggcttt cgttggacct atccattggg cacctgctga cttaagatta
     5161 tcaattgcct gtctactttc atctatctct tcggcgacat tctcttcttt aaggtgatat
     5221 tgacgtagaa gaagtgctgg atcgaatttt gcaaaaacag cacgtttctt tcgtccaaag
     5281 ttttttagtg tttttaacac aaagttaact aattctttat tgctgtagtc cataataggt
     5341 ccacgtggaa tataaagcat tgtaaaacca agtggtaatg atttaattaa gatgctagct
     5401 acagcaacta attggtcatc tttataaaag cctaaacgtt cattttgcca atttgatttt
     5461 acttgtgccc actttgaaga gtggaggaga ttggtttgag acgatgcaat agcaaaggca
     5521 tcatattctt tttcggatat tccaacggta acatgataca tcagtttatt acccattcac
     5581 gtagtgcgta ggctacgcca ctttcctcgt ttgatttggt gatatatttt gctatttttt
     5641 ttagttcagg attgccgttt tgcataacaa caggatttcc aacaacttca agcatagcac
     5701 ggtcattttc ttcatcacca atagccattg tttgatctac agttaatccc attttctctg
     5761 caaggtggag aagtgcagtg cctttattaa cgtttttggg taaaatttct aaataaaaag
     5821 gagttgattt tacaatagtg tagttatcga gaactttttt tggaatttta gcaattgctg
     5881 catctaagat gtcaggttga tcaatcatca ttagtttaat gatttctttg tctcccattt
     5941 cttcaggagt acgatagaag agcggcatat taactaaagt tacttcgtgt attgtatatt
     6001 taccaatatc acgatttgct gtgtaaattc cttcttttgt aatggcgtgc atgtggacgc
     6061 ctaatttacg cgctaacagt tcgaaatcca gatagtcttc ataggttaag gtttctttga
     6121 tgatgtcatc tcctgtggct gtgtcttgaa ctagaccgcc gttaaaggtg ataacataat
     6181 ctccctcttc attgagtttc aattcttcta aaagatcttg gactccgatg attggacgtc
     6241 ctgtagcaat aacaatttta acgccttttg attttgcttc ttgaacagct ttagccactt
     6301 caggtgtaat ttcacgctta ctatttaata gggtgccgtc aatatctacg gcaacaagtt
     6361 taattgaagt cattttatct cccactcttt attcaatatc ctaaaatact caccttatat
     6421 cttactaaaa aataaagggt ttgtcatgaa tggctattat ctttcgtgag tgtcaaatgc
     6481 tcatttgtga tataagattg aaattcttct tttgtacttg aaaataggct atctaatgtc
     6541 aacatttctt ttggaaaata aaaacgttga tcaccataat tagatccagt taatgctttt
     6601 acaatcggtg ataagcttga taattcagca agttgtccat tcttttggat aatttgaatt
     6661 tctgttcgtg gatttgaatg ttcaggccga taaacatcgt aaggtaagtc aaaattagca
     6721 tggacaccag tgtaataatc gggatcaaaa ccaacctgtg agactagttc tttcatttta
     6781 actagatttt ctttatcaga ctcttcaaag gtaattgatt taaagacttt tcgattgata
     6841 aaacgattag ctaaatctgc caaaatgtta tcatcagctt gcatccaaga ttggaagtaa
     6901 gtattcatta ccccatcatc tagggctaaa taatcttgta aatcatattg atccgtgaag
     6961 aaaggaataa gatttggaga agtttgttca aaaaaatcgc gatgggtatc aaataagaag
     7021 cgagcacgtt ttagtaaatt ttgtaagagt aattccatag cacgactggc tggatggaaa
     7081 taaacttgca tatacatttg gaagcgacta acaatataat cttccacagc gtgcatcccg
     7141 ttacgagcaa aagcaattcc agaatcggtt ggtctaataa cgcgtaaaat tcgtgttaaa
     7201 tcaaattgtc catagctagc agcagtgtag taagaatctc tgagaagata gtccattcga
     7261 tcacaatcaa tttgactaga aattaattga acaacttgtt tattggggta gctatgatta
     7321 ataacactag ctactttatc aggaaaatca ggagccacct ttcgtaaaat agcattaatc
     7381 tctgtggtag gattggtgat gatttcctga gtgtaagctt catgatcagt attgaagaga
     7441 cgctcaaatg tatgagaata tgcgccatgt ccgatatcat gtagtagggc ggcagccatc
     7501 gttagcaggg actcattttt attccaaaga tcagaataat gttcatcaaa gatttctgta
     7561 actttacgag ccagttcata aactccgaga caatgtgaaa aacgactatg ctctgcacca
     7621 tggaaggtaa aagaagtcgt tgaggtttgt ttaatgcgtc gtaaacgttg aaattcttta
     7681 gtgttaatca agtcatatat gacttgatta tttacatgga tataagtgtg aacggggtca
     7741 cgaaatactt tttcattcat atatatatta tagcaaaaat tgaaacttga gtcagaaatt
     7801 ggtagaatag aaggataaaa gaagtgagag aagtcatgaa actagaaatt aacaataaca
     7861 ttcaaattga caacgaaacc gaaatgattc atgagattca cgattgtcaa tttattgaaa
     7921 aaggaagtta cgtttatttg aattatatta atgctgaggg cgagagggta gttattaagg
     7981 caaatcatga ggaattgctt atgacgaggt tttcaaatcc taagtctgta atgcgttttc
     8041 atcgtgaaac gcctgctttg gttaatatcc ccactccact tggtgttcaa catttgatta
     8101 cagagacaag tcactatcag tttgatttgt cacagcaaag gttgcatatc aattatgtcc
     8161 ttaagcaaac ggagacagga gactgttttg caaattatga attaaggatt caatggtatt
     8221 aagataacta ccattaacaa aagagtagtc gttatgaatt tcgtttactg aagacgctgg
     8281 gatctcttta tgagagccat ttatttgata aactgcagtg tggtttattt cgactagatg
     8341 aacttcgttt tttcccttga gattattagt gatagcaagg ataattaatt gaaaaatgga
     8401 tttttttctg ttacaatgat taacatatca agggagtagc agacggttta ccgtggcaat
     8461 gtcgtcatta cgaaagaaat ttccggcgtt gtcttaaaca gcgagacttg ttttcataaa
     8521 aacaggtctc tttttgtttg tggagagacc aaaggaggaa aggttttgtt aaaagaacag
     8581 aaaaaatcct tgttttacac acaaggtcaa gaagaggttc taacgtcttt agaatcttct
     8641 cgtgaaggat tatcaacgac agaagcgaaa aatcgcttag agatgtatgg tcgcaatgag
     8701 cttgaagaag ggaaaaaacg tagtctaata gctaagtttt ttgaccaatt caaagattta
     8761 atgattatta tcttactggt tgctgcagct ctctctgtta ttacggaagg tatgcatggc
     8821 ttaacggatg cattgattat cttggcggtt gttatcttaa atgcagcttt tggtgtttat
     8881 caagaggggc aagccgaagc agctattgaa gccctcaaag atatgtcaag cccaattgcg
     8941 cgtgttcgtc gtgatggtca tactattgag gtggattcta aagaactagt tcctggtgac
     9001 ctcgttatgt tagaagcagg agatgttgtt cccgcagatt tacgactttt agaagccgcg
     9061 tctctaaaaa ttgaagaagc agctctaact ggtgagtcag tacctgttga gaaagacata
     9121 tctcaagtag tagcagagga tgcaggtatt ggtgaccgtg tgaatatggc gtatcaaaat
     9181 tcaaatgtca cgtatggtcg aggatatggt gttgttacta acacagggat gtatactgaa
     9241 gttggtaaaa ttgcggacat gttggcaaat gctgatgaat ctgaaacacc attgaaacaa
     9301 agtttagttc aactttctaa attattgact tacttaatcg tgattattgc ggttattact
     9361 ttcttagtgg ggatatttgt ccgtaaagaa ggttggattg aaggattgat gacttcagtt
     9421 gctttagcag ttgcagctat tccagaaggg cttcctgcaa ttgttacgat tgtcttatca
     9481 atgggaacaa aaactcttgc taaacgcaat tctatcgttc gtaaactccc tgcagttgaa
     9541 actttaggtt ctacagaaat cattgcatca gataagactg gaactctgac aatgaatcaa
     9601 atgactgtcg aaaaagtgta tactaatggt gttctccaat catcttcgga agaaatttca
     9661 gttgataata acactttacg tattatgaat ttctccaacg atactaagat tgatccttca
     9721 ggaaaattaa ttggtgaccc aacagagact gcactggtac aatttggttt agataagaat
     9781 tttgatgtcc gtgaagtttt gaaaaatgaa ccacgtgtag ctgagttacc atttgattca
     9841 gatcgtaagt taatgtctac gattcataaa gaatcagatg gtcgctactt tatcgcagtc
     9901 aagggtgctc ctgatcaatt attaaaacga gttactaaaa ttgaagataa tggcttggtt
     9961 cgtgatatca cggctgaaga taaagaggct atcctaaata ccaataaaga actcgcaaaa
    10021 caagctcttc gtgtcttgat gatggcttat aaatatgaaa cacaaattcc aactttagag
    10081 actgatattg ttgagtcaga tttagtattc tcaggtttag tgggaatgat tgaccctgag
    10141 cgtccagaag cggcagaagc tgttcgagtt gctaaagaag caggaattcg tccgattatg
    10201 attaccgggg accatcaaga tactgcagaa gctattgcaa agcgtctagg tattattgat
    10261 gcaaatgaca cagaagatca tgtctttacg ggtgctgaac ttaatgaatt aagtgatgaa
    10321 gagtttcaaa aagtatttaa acaatattct gtctatgcac gtgtttctcc tgaacataaa
    10381 gtgcgtatcg tgaaagcttg gcaaaacgat ggcaaagtcg ttgccatgac tggtgatggt
    10441 gttaatgatg ctccatcgct aaaaacagca gatattggta ttggtatggg aataactggt
    10501 acggaagttt ccaaaggtgc ttctgatatg gtccttgctg atgataattt tgcaactatt
    10561 attgtggctg ttgaggaagg acgtaaagtc ttctcaaata tccaaaaatc tattcagtat
    10621 cttctttcag ccaacatggc agaggtattt acaattttct ttgcaacttt attaggatgg
    10681 gatgtacttg caccggttca ccttctttgg attaacttgg taacagatac tcttccagcc
    10741 atcgctcttg gagttgagcc agctgaacca ggagttatga cacataaacc tcgtggtcgc
    10801 cagtcaaact tctttgatgg tggtgttatg ggagctatta tctatcaagg tattttacaa
    10861 actatcttag tacttggtgt ctatggttgg gcattgatgt atccagaaca tgctggttat
    10921 cgcatgattc acgcagatgc ccttacgatg gcctttgcaa cgcttggttt aatccaattg
    10981 gtacatgcat ttaacgttaa gtcagtttac caatcaatct ttacagttgg agcttttaaa
    11041 aatagaacct ttaactggtc tattccagta gctttcattc ttttgatggt gactattgtt
    11101 gttcctggat ttaataaact tttccatgta actcatttaa gttcaacaca atggttaact
    11161 gtagttatcg gaagtttatt gatggtagtg ttaacggaaa ttgttaaatt tatccaacgt
    11221 aaattaggac aagacgaaaa agctatttag tttagctgat aaaaaaactc acttagaata
    11281 tctaagtgag ttttttagca atcaaatatt gtcatagagt tgcgtaattc gtcttcaaaa
    11341 tatttttttg gatagttttc aaaactatcc aaatgcttaa cggcattata ccttttgcta
    11401 agattccagg ttaaattagg gttatgattg ggattctgat tgacaaagtc aatagttaaa
    11461 ttccattctc taatatatcc aagtggtcta gaatgataag tagtctctcc aattttaacg
    11521 tcgccaaatg agcggtgtgc atgaccaaaa acaacatctt tgatatgata tttttggaat
    11581 aaatcatgat aagcctgact ccccaaaaaa gcattaaaag gagagaatct aggatgagtc
    11641 atcgtgaatt gtttatgggg aacaaagtgc atagcaataa tgatatttga agaatcgact
    11701 tttgctaata tttcgtctaa gcgttttaaa attgatgctt ggatagttac atcattattt
    11761 ggacgtttta aacgtctatc aaaccaaaaa gtctttttta atttttcaac atccttgatg
    11821 tctctattat ttgaaaagct gtaatcatac cagccatgaa aagctagtag catttttttg
    11881 tcaaatcgat aggtttgaaa gtcaagacgt tggatttctg tttctgttaa atcaagcata
    11941 tcatggtttc ctaaattata gctaagttta atatgctttt ttaagttatt gataaaggga
    12001 agtgtatctt tagtaaaatg attagatata tctccagcga tatggaggtg atcaattttt
    12061 tctttcttaa gcaaattaat taacgttttc gtctcaaagg tagaaaaatg attgaggtca
    12121 atatgtaaat cactcatgat agcaagtttg gtcataaggg tattgtacca tattgtagtt
    12181 caaagagtta aacagtaaat ttttgaaata gttttagagt cattctataa tagatatgtc
    12241 attgtagaat gcgctttcat aagtgtgttt agaagtttta tatttaaggt gccttaagga
    12301 ggtctcatgt ctaattttta taagctcttg aaagaaaaat ttcctcgaaa agaagatatc
    12361 gtaacagaaa tgatcaattt agaagctatt tgtcaattac ccaaagggac ggagtatttt
    12421 attagtgatt tacacgggga atacgatgct gttgattatc tcttacggac aggtgcaggg
    12481 tctattagag ctaaattatt agactgtttt gattggcaga aaatagttgc tgttgattta
    12541 gatgattttt gtatactgct ttattaccca aaggaaaaat tagccttcga taaaatgaac
    12601 ttgtcagcaa gtgcttataa aacgaagtta tgggaaatga ttccgttaca aatacaagtt
    12661 ttaaaatatt tttctagtaa atatacaaaa tctaaagtac gtaagcaatt gtcaggtaag
    12721 tttgcttaca ttatcgaaga acttttagct gagattgacc gtaacccaga gaagaaaagc
    12781 tattttgata ctattattga aaaattattt gaattagacc aagtagaaga tttaattata
    12841 gtattgtccc aaacaattca agttttgatt attgaccatt tacatgttgt aggggatatt
    12901 tacgatcgtg gtaggtatcc tgatcgtatc ttgaataggt taatggcttt tccaaatttg
    12961 gatatccaat gggggaacca tgatgtcact tggatgggag cagcatctgg ttcttacctt
    13021 tgtatggtaa atgttatccg tattgctgca cgttataata atattacttt aattgaagac
    13081 cgatatggta tcaatttgcg tcgcttggtt gattactcac gccgttacta tgaacctcta
    13141 ccttctttcg ttcctatatt agatggagag gaaatgactc atccagatga actagacttg
    13201 ttaaatatga tacaacaagc aacagcaatt ttacagttta aattggaagc ccaattgatt
    13261 gacaggcgtc cagaatttca aatgcataat cggcaactta ttaaccaggt aaattataaa
    13321 gatttaagta tatctattaa ggaagttgtg catcaactta aagattttaa tagtagatgc
    13381 attgattcga aaaatcccag tcgattaaca agtgaggaag aagagctttt acaacaattg
    13441 atgatagcct tccagacttc tgaaagttta aagaaacata ttgatttcct atttgaaaaa
    13501 ggaagtatgt atttaacata taatgataat cttttattcc atggttgcat accaatgcat
    13561 agtaatggtg attttaaaag ctttaaaatt gcaggaaaaa cttatggtgg tagggattta
    13621 ctggatttat ttgaaagtca aatacgtcta gcatatgcta gaccagagaa acatgatgat
    13681 ttggcgacag atatcatttg gtatctttgg tgtggtgaaa attcttctct ttttggtaag
    13741 aatgccatga ctacttttga gcgctattat gtttccgaca aagtaactca tcaagaaaga
    13801 aaaaatccct actttaaact gagagataaa gatgatattt gcactgctct tttacaagag
    13861 tttgatttgc ctaagtttgg gcatattgtc aatggtcata cgccagtcaa agaaaaaaat
    13921 ggtgagcaac cgataaaagc aaatggtaag atgttagtaa ttgatggtgg ctttgccaaa
    13981 ggttatcaga aaaatacagg tttagctggc tacactttga tttataatag ctatgggata
    14041 caattaattt cacaccttcc ttttacatcc attgaggaag ttttaagtgg aaccaactat
    14101 atcattgata cgaagcgatt agtagaagaa gccaaggata ggattcttgt aaaagataca
    14161 actattggac aaaagttaac caaagagatt aaagacttag atcaccttta tcgtcatttt
    14221 caggaatatg atgactagtg tttaagtaat ttactaattg atatgtagga tttaattttt
    14281 cagtttctta ccttgtctct tttattaaat tgacttaaaa taatattatg gacataaagg
    14341 cagaaattca aaaattagca aaagaaattg gtatctctaa aattggtttt acgacggcag
    14401 ataactttga ttatttggaa aagtcattgc gtgcttctgt ggaagagggg cgcaattcag
    14461 gatttgaaca taaagtcata gaggatcgta tctaccctga aaggctttta gaatcggcta
    14521 aaacgattat ttcaattggc gttgcttatc ctcacaaact tcctcagcaa cctcaaaaaa
    14581 catcttataa acgaggaaaa attacaccta attcgtgggg gcttgattat cattatgtgg
    14641 tcggcgaaaa gttagacaga ctatcaaaag gcattgaaga gctctgtcga gattttcctt
    14701 tgcaacaaaa agcaatggtc gatactggag ctttagttga tactgcagtt gcccaaagag
    14761 caggtattgg ttttatagga aaaaatggtc tagttatttc taaagaatat ggctcttata
    14821 tgtttttggg agaattaatt accaatttag agatcgagcc agacaagcct gtcgattatg
    14881 attgtggtga ctgtcgtcgt tgtctagatg cctgtccaac ttcttgttta attggtgatg
    14941 gctcaatgaa tgccaagcgt tgtctatcat ttcaaaccca agataaggga atgatggaca
    15001 tagagtttcg aaaaaagatt aaaacagtta tctatggttg tgatatttgc caaatttgct
    15061 gtccttataa caaaggtata aataatccat tggcgacaga gattgatcct gagttggctc
    15121 aaccagaatt aataccattt ttgagtttat caaatggaca atttaaagaa aaatttggta
    15181 tgattgctgg aagttggcga ggtaaaaata ttttacagcg taatgccatt attgcgctag
    15241 caaatgctca tgataaaacc gcagtggtca aattaataga gattattgat aaaaataata
    15301 accctattca tactgcaaca gctatatggg cattgggaga aattgtcaaa aaaccaaacg
    15361 atgagatact ggagtttatg agtaatttaa ccttaaaaga tgaagatagc cgaaaagagt
    15421 tggaattaat ccgtcataaa tggcaatttt aagcctttta tggtattttt aagaacctta
    15481 tgataaaata aaacagatta acaaagattg gagaaatctc atggaagtgg ctgaaattcg
    15541 ccaaaaaata gtagaaaata aagagaagtt gactagcttc aggaggtctc tttgacttag
    15601 atcgtttgga agaggagatt gctcttcttg aaaatcaaat gactgagcca gacttttgga
    15661 atgacaatat tgcagcgcaa aagacatctc aagaactgaa tgagttaaaa ggtaagtatg
    15721 atactttcca taatatgcaa gaattatctg acgaaacaga actcttactt gagatgcttg
    15781 acgaagatga ttctttgaaa gaagaattag aagaaaatct aatgcagcta gataagataa
    15841 tgggtgctta tgaaatgacg cttctcttat ctgagcctta tgaccacaat aatgctatct
    15901 tagaaatcca tcctggttca ggaggaactg aagcgcaaga ttggggagat ttactcttac
    15961 gaatgtatac ccgttttggc aatgctaatg gtttcaaagt agaagtatta gattatcaag
    16021 caggggatga agctggtatt aaatcggtta ccctctcttt tgaaggcccc aatgcttatg
    16081 gtttgttgaa atcagaaatg ggagttcatc gcctcgttcg tatatcacct tttgattcgg
    16141 ctaaacgtcg tcatacctca tttgcttcag ttgaagtgat gccagagtta gatgatacga
    16201 ttgaagtaga agtacgtgat gatgacatta aaatggatac tttccgttcc ggaggagctg
    16261 gtggtcagaa cgtcaataaa gtatcaacag gtgttcgttt gacgcatatt ccaactggaa
    16321 ttgtggtatc gtctaccgtt gaccgtactc aatatgggaa ccgcgatcgc gctatgaaga
    16381 tgttgcaggc taagctatat cagttagaac aagaaaagaa agcacaagaa gtagatgctc
    16441 ttaagggtga taaaaaagaa atcacttggg gaagtcagat tcgttcttat gttttcaccc
    16501 cttatacgat ggtgaaggat catcgtacta actttgagct tgctcaggta gataaggtta
    16561 tggatggaga aatcaatggc tttattgatg cctaccttaa gtggcgcatt gaagactaaa
    16621 aataatgaag gagaaacatg gcactaatag aaatgtctgg tgttactaaa aagtatcgcc
    16681 gttcaacaac tgccttgaga aacttaaatt tatcaattca gcaaggtgag tttgtgtatt
    16741 tagttggtcc gtcaggtgct ggtaaatcta gcttgattcg cctcctatac cgtgaagaaa
    16801 aattaagttc aggtagatta aaggtagggg aattcaatct caataaactc aaacgtcgtc
    16861 aaattcctat tttacgtcgt agtattggag ttgtcttcca agattataaa ttactaccaa
    16921 caaaaacagt ttatgagaat gttgcgtttg cgatgcaagt tattggagct aagcgccgtc
    16981 atattaaaaa acgcgtgcca gaagttctag aattagtagg tttaaaacac aagatgcgtt
    17041 cttttccaac acagttatct ggtggagaac aacaacgtgt ggcaattgca cgtgcgattg
    17101 ttaataatcc aaaattatta attgcagatg aacctactgg taatttggat ccagaaattg
    17161 cttgggaaat catgcatcta ttggaacgta ttaaccttca aggaacgact gttttgatgg
    17221 caacacacaa tagtcaaatt gttaacacac ttcgtcatcg tgtcattgag attgaagcag
    17281 gtagcgttat ccgagatgaa gagaagggag aatatggtta tcatgattaa tttctttaga
    17341 catttctggg aatcgcttaa aaacttgaaa cgtaactttt ggatgacttt tgcttctgta
    17401 acatcagtta ccataacctt acttcttgta ggcttgtttt catcagtttt actgaatgtt
    17461 gaaaagttaa caactgatgt ttcaggtaat tttactatca gtgccttttt aaatgttgac
    17521 tcaacggatg ctcaaaaaca agtcaaagat aaggacggaa agctaaaaga taatcctgat
    17581 tatcataaag tttacgacaa aattaaacgt atttctggtg tagagaaagt cacttattca
    17641 agtaaagctg aacaattaaa agaagttcaa aaagagtatg gttcagatgt tattgatgat
    17701 acctataaag atgctttgtt agatgtttac gtagttggga caagttctgc aaaagtttct
    17761 aagagtgtca gtgaagcaat tggacgcatt gaaggtgttg attatactaa agaacctatt
    17821 gattcaacaa aactatctaa tctcacagat aacatccgta tatggggatt tggtggtgtt
    17881 gccttattaa ttgtccttgc tatctttttg atttcaaata ctattcgtat gtcgattatg
    17941 tcacgccgta cagatattga aatcatgaga ttagttggcg ctaaaaattc ttatattcga
    18001 ggaccgttct tttttgaagg tgcttgggta gggattttag gggctatcgt tccatcgctg
    18061 attttctatt ttggttatca atttgtgttt aacaagttta atccaaaatt tgagacaagc
    18121 catgtttcat tgtatcctat ggatattatg gttccagcaa ttattggtgg tatggtgatt
    18181 attggtatta ttattggctc acttgggtct gtcctatcaa tgagacgtta ccttaagatt
    18241 taataaaaag agatgaaatc tatttcatct ctttttatta attgtagata tcatgttagt
    18301 ggataaaatt cgaaataaac gtagccaatt gtttttcaga tgatttgtga gaaagggtag
    18361 ctttaccatc ccctttttga tgtccgtagt taccaaatcg gctatggttt cctccgacaa
    18421 tagttcgaaa agtacttgaa tttgggaggc gttttttagc ttcctcatat ttttcccagt
    18481 ttaaaatatg atcattactt gctgtaatgg agagtactct taacttttta tgagataggt
    18541 cggatttacg tgaagggtag gaagctaata atatgagccc tctaacaggg gctactttgg
    18601 cattttggct agcaacaact cctcctaaag aatgtccagc aaggtaaaca ttagttaagt
    18661 ggttctgatt aataattgtt ttagctttat gaggcgataa aactggcaaa tttaaaggtg
    18721 tcttcaaaat ataggtatta taccctttgt cagctaaatc tctagcaagt tgagaataag
    18781 cttcttcttc cacaagagcc ccttgataga aaataatatt tgcttttacc ttacccttgc
    18841 ttctaaaaag aaggtagttc ttatggtttg tagcggtttt agcaattctc ctagctaatg
    18901 gggaggcttg gtaagtattg aagtaaatgg tcatcccgac ggccattaaa atagcaagga
    18961 tacttgttgt taaccaaaga aaaatagagt gacgttttat gttcattgga agaaaggatt
    19021 aaagtttttt tcgtggccaa tagttgtatt catgccatgg cctgggtgaa cactataatg
    19081 actaggtaag gtgaatagtt cttgacgaat accagtaatg aggtcctcaa agttactggt
    19141 aggtaagtca gtacgaccga tagtttcacg aaatagagca tcgccagtaa cgactaattc
    19201 atctgagtgg aaaacaaacg agactcctcc ccaagaatga ccaggtgttg gtaaaactgt
    19261 gaattcaaaa ccatttagtt gatagggttg ctttaattta aaaaagttct ctgccggtct
    19321 tgctataaca ttaatgatat catcatgacg gcctaaccca gataaattat catctgggga
    19381 agatagccaa gcggcttctt tttctgaaac ataaacaggt ggattatcaa aagtatctct
    19441 gaccaaatct aaactaaaaa tatgatcata gtgagtatga gttaagagga tagccactaa
    19501 aggtttttca aaggatttaa ttttagcaat aatttcttgg ccattgctcc ctggatcaat
    19561 gaggataaca gcttgatcat taacgaggta ataagtattt tcaccagcaa tgtggtttat
    19621 tgttttatag attttcataa tgatagtgta ccaaaaaatc aagactttgt taaaaaaaga
    19681 gtggcggaaa ataaatggca actagtttca taaaatatgg gatctcacag caatttcttg
    19741 acaccgtttt cataaaggtg tatcataaaa gtaatcaata agccaagcct aaagctgaac
    19801 ttagtttaag aacactgact ttttgagagc aacctaattg gttgactaaa gaatatgagg
    19861 tggaagatat gtcaaaagtt gctattgtta ctggtgctgg tcaaggaatt ggatttgcta
    19921 ttgcgaaacg tcttcatgca gacggcttta aaataggcgt tttagactat aatgaagaga
    19981 ctgctcaggc tgcggttgat aaactatcac ctgaggatgc tgttgcagtt gtagcagatg
    20041 tttcaaaacg tgatcaagtt tttgatgctt tccaaaaagt tgtagatact tttggtgatt
    20101 tgaatgttgt tgttaacaat gcaggtgttg ccccaacaac tcctcttgac actattactg
    20161 aagagcagtt tgaaaaggct tttgcaatca atgtcggagg aactatttgg ggaagccaag
    20221 cagctcagaa acattttaga gaattaggtc atggcggaaa aattataaat gcaacttcac
    20281 aagcaggatg tgaagggaat cctaatttga ctgtttatgg tggtacaaaa tttgcagttc
    20341 gtggtattac acagactttg gcaaaagatt tagcatcaga aggaatcaca gtcaatgctt
    20401 acgcaccagg tattgttaaa acacctatga tgtttgatat tgcccatgaa gttggtaaga
    20461 atgcaggcaa agatgatgaa tggggtatgg agcagtttgc caaagatatc actttgaaac
    20521 gcttgtctga accagaagat gttgctaatg cagtaggctt cttagcggga gatgattcaa
    20581 attatatcac tggacaaaca atagttgtcg atgggggtat ggtattccat taaaaatgat
    20641 taatgctaac actgtgagtt tcactcacag tgttttttat atttcttaaa aaattatctt
    20701 atcttggtta ttggagtgat ggcatctttg aggtatagtc atgttttgtt ttatagatat
    20761 agcgtgctat aatagactta ctatgaccca gaaaaaactt agaaaatatg cagtagttga
    20821 cttggaggct actggagcag gtcccaatgc ttctattatt caggttggga ttgttattat
    20881 ccaaggaaat aaaattattg atagttatga aacagatgtt aatcctcatg aatctttgga
    20941 tgagcatatt gttcatttga caggtattac ggataagcag ttggctaaag ctccggattt
    21001 tggtcaagtg gcgcatcaca tttatcaatt gattgaagat tgtatttttg ttgcccacaa
    21061 tgtaaagttc gatgcaaatt tattggctga gcaactattt ttagaaggct gcgagttacg
    21121 tacaccaagg atagatacag tagaattatc acaagtattt tatccctgtt tagaaaaata
    21181 ttctttaggg gcattagcag aaagcttaaa tattgaatta acagatgcgc acactgctat
    21241 tgcagatgca agagctacag cacagttatt cattaagtta aaagctaaaa ttagtagttt
    21301 acctaaagaa gtgttggaga ccattttaac atttgctgat aatcttttgt tcgaatccta
    21361 tttattgatt gaagaagctt atcaagaagc tgatttcgtt aatcctaaag aatattattt
    21421 ctggcaagga ttggtcttaa aaaaagaaaa agctgttgga aagcctaaga aattatctag
    21481 tgattttcaa gttaacatgg ctttgttagg tatggatgct cgccctaagc aagttgtttt
    21541 tgctgacttg gttaaagcgc attttaatga tcaaacaaca acgtttttag aagctcaacc
    21601 aggacttggt aaaacatatg gttatctctt gcctttattg gatcagtctc aaaaacaaca
    21661 aataatagta tctgttccaa caaagatttt gcaagatcaa ataatggcta aagaaataaa
    21721 gcacattcaa gaactttttc atattccctg tcacagtatc aaaggaccga gaaattactt
    21781 gaagttagat gcgttttata agagcctcca agtgcaggat cgaaatcgat tgataaatcg
    21841 ttttaaaatg caactgctag tgtggttgac ggaaacgact acaggtgatt tggatgaaat
    21901 taaacaaaaa caacgattag aatcttactt tgaccagcta aaacacgatg gagaagtgac
    21961 tcaatcatca cttttttatg atctggattt ttggaaacgc agttatgata aagttgcaca
    22021 aagtcaactt gttattatca atcatgctta ttttcttgag cgtgttcagg atgacaaaga
    22081 ttttgcaaag gggaaggttt tagtttttga tgaagctcag aaacttgttt tgggactgga
    22141 aaatttttct cgtgggcaac ttgatattag tcaccaattg caggttattc aaaaaataat
    22201 tgatagttct attccattat tacaaaagcg ccttctggaa agtatctctt atgagctaag
    22261 tcatgcagta gaactttttt atcgtcataa ttcttttgaa ttttcagaga cctggttgaa
    22321 acgtctaaaa aatagtataa acgctttaga agtagtagga ttagatgagt tacaaacttt
    22381 tttcacagct acttatacta actattggtt tgaaactgac aaagtcaatg agaagcgttt
    22441 aaccatttta cgtggggcga gagaagattt tcttaaattt tcaaagttcc tcccccccac
    22501 aaaaaagacc tatatgatat cagcgacttt acaaatcagt cccaaagtct acttgtcaga
    22561 tcttttagga ggattttcaa gtataagtac agaaaagata gcgcatgaga aaaatgccaa
    22621 tcagaaagta tggattgata cctctatgcc caatatactt gatttatcac cggaacagta
    22681 tgcttacgaa atagccaagc gtttacaaga tataatgacg ttaaagcagc ctaccttagt
    22741 cttattaact tctaaacaga caatgtttat ggtatctgat tatttggata agtgggagat
    22801 aaaacatctt actcaagata aaaatggctt agcttataac gttaaaaaac gttttgatcg
    22861 tggagaaagc aatcttcttt taggtacagg atctttttgg gaaggtgttg actttgtaca
    22921 tcgtgatcgt cttattgaag ttatcacgcg attgcctttt gataatccca aagactattt
    22981 tatccagaaa ctatcgcaga gtttaacaaa agaggggaaa aacttctttt atgactattc
    23041 tttaccaatg actgtcttaa aattaaaaca ggccttggga cgtactacaa ggagagaaga
    23101 acaaaagtca gcggtaatta ttttggatag cagattggtt attaaatctt atggacaaac
    23161 catcatgcat tctcttggta gagattttga aatttctaag gaaaaaatta acaaggtctt
    23221 gactgaaatg gctaaatttt tgatataatg gtaaagatca ttgtctaaaa ataagatttt
    23281 gacagtgcga ctcaagatta aataatagta gtacgttgat aaaaggagga gtaatgacct
    23341 atttgtcaga acgtgcactc aatatggaag agagtgttac tttagctgcg ggagctaaag
    23401 caagagaact cagagtacaa ggaagagata ttcttagttt aactttaggg gagcctgact
    23461 ttgctactcc taaaaacatt cagcaagcgg ctattgaggc tataacagat ggacgtgcaa
    23521 gtttttacac accatcaagt ggtttgccgg agttgaagtc agcaattaat gcgtattttg
    23581 aacgttttta tggctactcc cttaaaccta atcaggtggt tgtgggaact ggtgctaagt
    23641 ttatccttta tactttcttt atgacagtac tgaatcccgg tgatgaagta attattccga
    23701 ctccttattg ggtatcttat gctgatcaga ttaagatggc tgaaggtaag ccagtttttg
    23761 tcacagcaaa agaagttaat cacttcaaag taactgtgga acagttagaa gctgttagga
    23821 ctgataagac aaaagtcata cttcttaata gtccatcaaa tcctacaggt atgatttata
    23881 aagctgagga gttggaggct attggaaatt gggcagtcga acatgatatc ctaattttag
    23941 cagatgatat ttacggtcga ttggtttaca atggcaatat ttttacccct atttcaagcc
    24001 tttcagagtc tattcgaaac caaacgatag tcataaatgg agtttctaag acttatgcga
    24061 tgacaggttg gcgtgttggt tttgcagtgg gtaatcatga tattatagcg gcaatgtcta
    24121 aagtagtcag tcaaacaact tctaacctta cggctgtctc acagtatgct actattgagg
    24181 ctttaaatgg ttctcaagag tcatttgaaa aaatgaggct agcatttgaa gaacgcctta
    24241 atatcattta tccattgttg tgtcaggtcc ctgggtttga ggttgttaaa ccacaaggtg
    24301 ctttttatct tttcccaaat gttacaaaag caatggaaat gaaaggatat acggatgtta
    24361 cagcattcac ggatgccatt cttgaagaag ttggacttgc tttagtaacg ggtgctggtt
    24421 ttggagcgcc ggaaaatgtt cgtctgagtt atgctacaga tcttgaaacc cttaaagaag
    24481 ctgttcgtag actgcatgtc tttatgggaa gtaatgaaat taattaaata aaaatagaga
    24541 gaaacttatg tcaaaaaaac ttatttcaat tgttgatgtt aaagattatg ttggtcaaga
    24601 agttactatt ggtgcttggg ttgctaacaa atcaggtaaa ggtaagattg cgtttgtaca
    24661 gttgcgcgat ggttctgcct ttttccaagg tgtcgctttt aaaccaaact tcattgaaaa
    24721 atatggtgaa gaatcaggtc ttgaaaaatt tgacgttatt aaacgtctca accaagaaac
    24781 atctgtttat gtaacaggga ttgttaaaga ggatgaacgt tcaaaatttg gctatgagct
    24841 tgatattact gaccttgaag tcattggaga gtcacatgaa tatcctataa ctcctaaaga
    24901 gcatgggaca gacttcttga tggacaaccg tcatctttgg ttgcgttctc gtaaacaaat
    24961 ggctgttatg caaattcgta acgctattat ttattcaact tatgaattct ttgaccaaaa
    25021 tggttttatc aaatttgata gcccaatttt atcagaaaat gcagcagaag attcaacaga
    25081 gttatttgaa acagactatt ttgggaaacc agcatttctt agccaatctg gtcaattata
    25141 ccttgaagct ggagcaatgg ctctaggccg agttttcgac tttggtccag tctttcgtgc
    25201 tgaaaaatct aaaacacgtc gccatttaac agaattttgg atgatggatg ctgagtattc
    25261 tttcttgtca catgaggaat cacttgacct ccaagaagct tatgttaaag cattgatcca
    25321 aggcgttcta aatcgtgcgc ctcaagcact tgatatttta gaacgtgatg ttgaggctct
    25381 caaacgttat atcgcagagc cattcaagcg tgtttcttat gatgatgcta ttacacttct
    25441 tcaagagcat gaagcagatg aagatacaga ttatgaacat ttagagcatg gtgatgactt
    25501 tggttcacca catgaaactt ggatttctaa ttactttgga gtgccgacat ttgtagtgaa
    25561 ttacccagca agctttaaag cattttatat gaaaccagtt cctggaaatc cagaacgcgt
    25621 tctttgtgct gatcttctag ctccagaagg ttatggagag atcattggcg gctcaatgcg
    25681 tgaagacgac tacgatgctc ttgtggctaa aatggatgaa ctaggtatgg ataaatcaga
    25741 atacgacttt taccttgacc ttcgtaaata cggctctgtt cctcatggcg gttttggtat
    25801 aggtattgaa cgtatggtaa cttttgttgc tggtaccaaa catattcgcg aagctattcc
    25861 attcccacgt atgttacatc gtatcaaacc ataataaaaa agcgctagct agtgcgcttt
    25921 tttattatgg tctttagaat cgttggttaa ttaaattggg actatgtttt tttaacaatg
    25981 tgattgtcga aaagacaagg ataagactga ttagtcgatc catataatct gttccaactt
    26041 gaacaagtat caaactactt gtcatgtcta atccaattcc atgtaagaat tgtgctataa
    26101 tacttgagcc actagaggta attcctttaa aaaggataac tgttataact gaagcaataa
    26161 tagttcctgg taaagaaata atcagtgatt tccacaatag gctagagggt ttacaattcc
    26221 ttttaatcaa gatacccgta atgatagcaa ccacgatggc gactggtgaa taataaagtg
    26281 aaaaaatatc agtagtcatc caattgatga gtgcactgag aaaagcagtg ctggcagcgt
    26341 accatggtcc caggataact gctattaaca atgtacctat agtatctaga tagataggta
    26401 ggcgtaaaaa tagagctaaa ttagccccaa caaggttgat ggcgatagcg attgagataa
    26461 atgaaattaa acgagcaggt gattttgtca tttgttaagt cctaacattt ctaagtcttc
    26521 aatgatgact gaattttgtg catgtaatag gacggtcata aaatcattcc aaaataaatt
    26581 agtattgact gaggttaaaa tcttactgtt agcatctttt ttataaaaat gatactgatc
    26641 tacaattgtt tgtcccatgg cgattccgtg acaagcaaca tcggtatagc tatcaaaacc
    26701 agtagcgata ttttcattaa caaagtaagc aatagctaaa ggatcattta tcacacatcc
    26761 aataatatgc tcatattgcc agtgaaaatc gaagtagaac tttgtgattt tttggataaa
    26821 acttgatacg tctggattaa tacgctccat ataagataaa tgattggggg ttaatacaat
    26881 atgacgtgtg atgtctagtc caaccatctc tatttttttg tcaaggtttt caaatacata
    26941 ttgtgcagca tgaggatcac accaatagtt gtattcagca actggagaac aattgccatg
    27001 tgatttaaaa cttcctccca tgctaataaa tcgcttgcaa tgtttgccta atttgggatt
    27061 agtttgtaag gctcgagcga tattggtcag aggtccgaga gctataatag aagtatcatt
    27121 tttgtgttca aaatagtttg ctaaaaaaca atctgctgat tcttcttgaa agataattgg
    27181 ttgcgccaat gtaaagttat tctctccaag gccatccatt ccatgagtat cctgagcaga
    27241 tacaaattca cgttgtaaag gcaagttatc accaacataa actggaatgt cgtgacggtt
    27301 tagtaattca agcgttacaa aggtattctt tagtcctagc tcaacaggcg aattgcctgc
    27361 tgttatagta atagcaacaa cttctaactt aggatgctga atagcataca tcagtgctaa
    27421 ggtatcatca ataccgggat cgcaatcgat aataattttt tctttattca tttgtctcct
    27481 ttcttttaat attagtgaat gttctatgct tttatgaaac tattaacaat agtcatggca
    27541 ataaaaaaga tcttggcagt taccaagatc acacgaaaaa atgaatttag ttttctaaat
    27601 tcaatcatat tttcagtcac ttagtttttt attctgggaa gttttcgaac cagtccgaga
    27661 aatactcgat tatgcacgtg ttttattata atatgaaagc actaacttgt aaagattcaa
    27721 actgaccatt taacagtgtc tagattgatt gttttagcat ttgaaattaa ttttgaaatc
    27781 ggacatctgc tgtgtgcttt gttaacgatt agcttactat cagataatga cagtttttca
    27841 atactagcaa cagcgttaac ttggaaataa aatcccttac ctacttgctt ttccttcatg
    27901 agctggcaag taacatcaac gcgagatttt aggtctttga atcctttttg ttctaaaata
    27961 gcttttatag ttgcatttaa acatgttgac catgctaaag caattaattc ctcaggatta
    28021 aatcctggta aatgatttaa agggtgattg gtctccacaa tttttccatt ggagagataa
    28081 acttttccat caacgccttg agtgtttgat gaggttactt cataaagcat aataaactcc
    28141 tttgggtcta gtttaccaaa tttgggagca ttccgacaag attttactat ttagaatgag
    28201 tttcttttcc tctgaagtaa aatcctaatt gctagccatt tgtggtataa tggcataaaa
    28261 cataagagaa agaggtaatc gtaatgtcag acgaacaaat caaacttgtg attgtaacag
    28321 gtatgagtgg ggcaggaaag acagtggcta ttcagtcatt tgaagattta ggatatttta
    28381 caatagataa tatgccgcct actctagtgc ctaagttttt agaattagca gcacaatcag
    28441 gagatacaag caagattgct atggtagttg acatgcgtag tcgtcttttc tttagagaaa
    28501 ttaactctat tttggatagc cttgaaatta atgataatat taactttaag attctatttt
    28561 tagatgctac tgacaccgag ctagtgtcac gatataaaga gacacgtcgt agccaccctc
    28621 ttgctgccga cggcagagtg ttagatggta tttctctaga gagagaacta ttagcaccgc
    28681 ttaaaagtat gagtcagaac gttgtggaca ctagtgaatt aacaccaaga caactacgta
    28741 aggtcatttc taaagaattc tcaaatcaag atagtcaaag ttcttttcgt atcgaagtta
    28801 tgagttttgg ttttaaatat ggtatacctt tggatgcgga tttagtattc gatgtgcgtt
    28861 ttttaccaaa tccatactat aaaccagaat taagagataa gacaggttta gatactgagg
    28921 tttatgacta tgtcatgagt tttgatgaat cagatgattt ttatgaccat cttcttgcct
    28981 taataaagcc aatcttacca ggctatcaaa atgaagggaa atctgttttg actgtggcaa
    29041 tcggatgtac aggtgggcaa catcgtagca ccgcttttgc tcatagatta tcagaagatt
    29101 taaaagcaga ttggacagtt aatgaaagcc atcgtgataa aaataagcgt aaggagacgg
    29161 taaatcgttc atgagaaaac ctaaaataac cgttatcggc ggaggaacgg gaattccagt
    29221 tattttgaaa agtcttcgct tagaagatgt tgagattaca gcggttgtca cggttgcaga
    29281 tgatggtggt tcgtcaggtg agttgcgctc agttatgcaa ctaacaccac caggagatct
    29341 aagaaatgtt ttggttgcct tgagtgatat gccgaagttt tacgaacaaa ttttccaata
    29401 tcgttttgca gaaggtgatg gggattttgc aggtcatcct ttaggaaatc ttattattgc
    29461 tggtgttgct gaaatgcaag gttcaactta taacgctatg cagagtttaa cacagttctt
    29521 tcatacaaca ggaaagatat atccatctag tgaacatccg ctcactctgc atgctgtttt
    29581 taaagatggc catgaagtgg ttggagagag tcaaatagcc gattataaag gcatgattga
    29641 ccatgtgtat gtcactaata cttataatga agagactcct acggccagcc gcaaagttgt
    29701 tgatgcaatt ttagaaagtg atatgattgt gttggggcca gggtcacttt tcacctctat
    29761 tttacctaac ctagttattc cagaaattaa acaagctctt ttagaaactc gggcagaggt
    29821 agcttatgta tgcaatatta tgacacaacg tggtgaaact gagcatttta cagacgcaga
    29881 ccacgttgaa gtattgaaac gtcatttagg acaagatgct atcgatacag ttttggttaa
    29941 tattgaaaaa gtgccagagt cttatatgga aaacaatcat tttgatgagt atttggtaca
    30001 ggttgaacat gatttttcag ggcttagaaa acatgccaga cgtgtcatct cgtctaactt
    30061 tttaaaatta gaaaaaggtg gagccttcca tcatggtgac ttcgttgttg aggaactaat
    30121 gaatttagta ggaacacatt tatgagtttt acagtaaaag tcaaagaaga gctgctaggt
    30181 cataagagtg aaaataagat ggaactgtca gctattatca aaatgtcagg tagtttagga
    30241 ttagctaacc acggccttaa cctttctatt actacagaaa atgctaaaat tgctcgtcat
    30301 atttatagca tgctagaaga acattatcat ttgcagccag aaatcaaata ccatcaaaaa
    30361 acgaacttac gaaagaatcg tgtgtatact gtttttattg aagaaaaagt tgatgttatt
    30421 cttgctgatt tgaagttggc tgatgctttt tttggtattg aaactggtat tgaacactct
    30481 atattggaca atgatgaaaa tggaagagct tatctcaggg gagcattctt atcaacgggt
    30541 actgttagag aacctgattc tgggaaatat caactagaaa ttttttctgt ttatctagac
    30601 catgcacaag acttagctaa tcttatgaaa aaatttatgt tagatgcaaa ggttattgaa
    30661 cataaacatg gagcagtaac ttatttgcaa aaagcagaag atattatgga tttcttgata
    30721 gtcattgatg ccatggaagc tagagatgct tttgaagaga ttaaaatgat acgtgagaca
    30781 cgtaatgata ttaatcgtgc taacaacgtt gaaacagcta atattgctag aacaatcaca
    30841 gctagtatga agactatcaa taatattatc aagattatgg atactattgg ttttgatgca
    30901 ctgcctagtg atttaagaca agtagctcag gtgcgtgtgg ctcatccaga ttactctatt
    30961 caacaaatag cagacagttt agaaacgcct ctgagtaaga gtggcgttaa ccatcgctta
    31021 cggaaaataa ataaaattgc agatgaatta tagaaggaga tattcacatg gcttgtacaa
    31081 caatattggt tggtaaaaag gcttcttatg atggttcgac tatgatcgct agaacggaag
    31141 actctgttaa tggcgatttc acacccaaaa aattaaaggt aatgacatct aaagatcaac
    31201 cgcgtcatta caaatcagtt ttatcaaatt ttgaagtaga tttaccagat aacccacttc
    31261 cttatacttc agtaccggac gcattgggaa aagatggtat atggggtgaa gccggtatta
    31321 acagtaaaaa tgtagcgatg agtgctacag aaactattac aacgaattcc cgcgttttgg
    31381 gtgcagatcc tttggtttca gatggtatag gggaagagga tatactcact ttagtgcttc
    31441 cctatattca gtcagcgcga gaaggtgtgg agcgtttagg tgctattttg gaaaaatatg
    31501 gaacctatga atcaaatggt attgcttttt cagataccga agaaatatgg tggttagaaa
    31561 caattggtgg gcatcattgg attgctcgtc gcgtacctga tgatgtttat gttactaatc
    31621 ctaaccaact aggaattgat cattttgaat ttaataactg tgatgactac atgtgctcta
    31681 gtgatttgaa agagtttatc gaacaatacc atttagattt gacctattct aatgagcatt
    31741 tcaatcctcg atatgctttt ggtagccaac gtgataaaga tcgtcattac aacacaccaa
    31801 gaagttgggc aatgcagcgt tttttaaatc ctgaaattga acaggatcca cgtagcttgt
    31861 ttattccctg gtgtcaaaag ccttaccgaa aaattactgt tgaggatatt aaatatgtgt
    31921 tgagtgatca ttatcaagac agtgtgtatg acccatatgg accagaaggg gatgcggtaa
    31981 gtaggagagc ttttcgttca attggtatca accgaactag tcaaacgtct attctacaat
    32041 tacgaccaaa taaatcactt gaaacgacag gtgttcaatg gttatcttat ggctctatgc
    32101 catttgcaac catggtgccg ttgtttacac aagttgagac tgtaccaaac tatttttcga
    32161 atacaaccaa ggatgcttca acagataatt tttattggac caatcgttta attgcagctc
    32221 tagcagatcc acacttttat caacatgaag ctgatattga aagctatatc gagagaacga
    32281 tggctcaagg acatgcacat attaacggtg ttgatagaga agttgctgag aataaagaga
    32341 ttgattttca acagaaaaat caagaaatga gtgactatat ccaaaaagaa agccaagaat
    32401 tgttaaatcg tattctattt gatgcaagta atttaatgac aaatcgcttt tcaatgggag
    32461 attaaaaaac tcggaatatt ttccgggttt tttaagataa aaacgcttgc ataagtatat
    32521 aaaacttgca aaaaaaaaat atcgtgctat actttactag taaaatatta accggtaaat
    32581 aacgggttaa ataaaaagga gaaaaaatga gaaagaaatt tcttttattg atgagctttg
    32641 tagctatgtt tgcagcttgg caacttgttc aagttaaaca agtttgggct gatagtaaac
    32701 ttaaagtggt aacaactttt tacccagttt atgagtttac aaaaaatgtc gttggtgata
    32761 aagctgatgt atctatgtta attaaagcag gtacagaacc gcatgatttt gaaccatcaa
    32821 ctaaaaacat cgctgccatc caagattcaa atgcttttgt ttacatggat gataacatgg
    32881 aaacttgggc tccaaaagta gctaagtcag ttaaatccaa aaaagtaaca actattaaag
    32941 gtactggcga tatgttactt actaaaggcg tcgaagaaga aggtgaagaa catgaaggac
    33001 atggtcatga agggcatcat catgaacttg acccacacgt atggttgtct ccagaacgtg
    33061 cgatttctgt tgtagaaaac atccgtaata aatttgtcaa agcttatcca aaagatgcag
    33121 cttcatttaa caaaaatgca gatgcttaca ttgcaaaatt aaaagagctt gacaaagaat
    33181 acaaaaatgg tttgtcaaat gctaaacaaa agagttttgt gactcaacac gcagcgtttg
    33241 gttacatggc gcttgattac ggtttaaatc aagttccaat tgctggtctt actccagatg
    33301 cagaaccttc atcaaaacgt ttaggcgaat tagctaaata catcaagaaa tataacatca
    33361 actacattta ttttgaagaa aatgcttcaa ataaagttgc taaaacttta gcagatgaag
    33421 ttggcgtgaa aacagctgtg cttagtccac ttgaaggact ttctaaaaaa gaaatggcag
    33481 ctggcgaaga ttacttctca gttatgagac gtaatttgaa agttcttaaa aagacaacag
    33541 atgttgcagg taaagaagta gctcctgaag aagataaaac taaaacagtt gaaacaggtt
    33601 actttaaaac taaagatgtt aaagaccgta aattgacaga ttactctggt aattggcaat
    33661 cagtatatcc tcttcttcaa gatgggacac ttgatccagt ttgggattac aaagctaaat
    33721 ctaaaaaaga tatgactgct gcagagtaca aaaaatatta tacagcaggt tacaagactg
    33781 acgtagaatc aatcaagatt gatggtaaaa aacatcaaat gacctttgta cgtaatggta
    33841 aatcacaaac atttacatac aaatatgcag gttacaaaat cttaacttat aaaaaaggta
    33901 atcgtggagt acgttatctc tttgaagcta aagaaaaaga tgctggtcaa ttcaaatata
    33961 tccaatttag tgaccatggt attaaaccga ataaagctga acacttccat atcttctggg
    34021 gttcagaaag ccaagaaaaa ttatttgagg aaatggaaaa ctggccaaca tacttcccag
    34081 ctaaaatgtc tggacgtgaa gttgcccaag accttatgtc tcattaatat ttaaaagatg
    34141 atatcggata cgatatcatc tttttttctt tgtacgtgat aggctcaaac atagaaaaaa
    34201 gcacttctaa tagaagtgct tttaaaatta atgctatgct tattgagcag cgtttgcgtc
    34261 tttgagacca tattttttgt tgaaacggtc cacacgtcca tctgcttgag tgaatttttg
    34321 acgtcctgta tagaatggat gtgaatctga tgagatttcc acacgaataa gtgggtaagt
    34381 ttcaccttcg aattctactg tttctttagt tgatttagtt gaaccactaa ggaatttgta
    34441 acctgtagtt gtatctaaga aaacaactgg acgataatct ggatggatgt cttttttcat
    34501 ctttaaaaat ttcctttctg ccataactcg tctgagtcat agttattaac cactctattc
    34561 tatcaaataa gaatagtctt gacaagtgtt ttgataaaaa aagtaaaatt ataaagaaag
    34621 tatttcctgg atttcttggt aaatagcttg attctcttcc aaactataag aattagctcc
    34681 gctggctaaa ggatgtccgc caccattatg acgtttagct atttcattaa tgatgtgtga
    34741 cttactacgt aggcgcacac gataatgccc atcgctttgt tcaacgaaaa ttgcccaact
    34801 ttctacaata tcaatttttc caggtacacc aacgattgca gcagtctctg catctgttac
    34861 atcgaagcgt tttaggtctt cttgtgttaa agtaacgcat gcagcgccgt tcttgtcaat
    34921 tttaagttgc tcaaaaataa agccttgtaa ttttgcaatt ttgaaaggaa aggaatccat
    34981 ttgacgtgcc attgcagaaa aatcaaagtc aaattcacga agtttgctag caatcttcag
    35041 tgttttactt gtggtagcag gatataaaaa gcgtcctgta tctcctacaa taccattata
    35101 tagaactctt gctgcacttg ttgataacaa caaatcacaa gaaagagcaa agtctgtcac
    35161 aatttcactt gcactagagg cgttagtatc tacataagag agatctccat agacttcatc
    35221 gtttggatga tggtcaattt taattaaaaa gtcacctttt ttataacgtt catcatcaat
    35281 gcgtggggta tttgctgtat ctgtaacaac aacaagagcg ccttgatagt cttggtcggt
    35341 cacttgatcc attttagcaa tccaagcgag agtgggttcg tcaaaaccag tagcaagtac
    35401 ttttttcttt ggaaaattat gcctgatgat atcccttaag ccgatttggc taccaagagc
    35461 atcagggtct ggtctcatat gtcgatggat aatgatggtg tcgtactctt tgattttatc
    35521 aagaatttgt tgaaaaataa tcatttttta cctacctaac tgttttgtct tttagtgaag
    35581 aacaaggcta tattgttgac tatattatag cttaaatctt ttattgagga aagaaattaa
    35641 gatagcggtc acaatgtttg tttattttta tatgatggta tgttattttt ttataaaatt
    35701 cggatactgc taactaataa tcttttttat aataatattt tgtaaatgta ttgcttttta
    35761 gctaactttt gttaatatag taaaaatgct ttttaatagg agataaatta tgacacaagc
    35821 agtattaaaa gaattggcta aggcggagtt gcattgtcat ctagatggct cactttcttt
    35881 accagctatt agaaaattgg ctaatatggc tgatataata ctgccgagta gtgataaaga
    35941 attaagaaaa tatgttatag ctccagctca aacagaaagc ttggtagatt accttaaaac
    36001 gtttgagttt atcagaccgc ttcttcaaac gaaggaagca ttacgttttg ctgcttatga
    36061 tgttgctaga caggcagctt tagaaaatgt tatttatatt gagattcgtt ttgcgccaga
    36121 actctctatg gataaaggtt taactgcaag tgatacagtc cttgcagtct tagaaggttt
    36181 agcagacgct cagaaagaat ttaatatcgt tgcacgcgcc cttgtttgcg gtatgcgcca
    36241 atcgtctcat aaaacgacca aggacattat taaacacatt gttgatttgg ctcccaaagg
    36301 cctggtaggt tttgattttg caggtgatga attttcctat ccaacagata gtttagtaga
    36361 tcttattcag gaagtaaaac gctcaggtta cccgatgact ttacatgcag gtgagtgtgg
    36421 ttgtgctaag catattgcgg atagtttaaa tttagggatt aagcgtatgg gacatgtgac
    36481 agctttaaca ggacagaggg atttaataaa gcgatttgtc gaagaagatg ctgtagcaga
    36541 gatgtgttta actagcaatt tacaaacaaa agcagcctca agtattcaaa gttttcctta
    36601 tcaagaactt tatgatgctg gaggtaagat tacaattaat acagataatc gtactgtttc
    36661 cgatacaaat ttgacgaaag aatatagtct tttcgtaact tattttggaa cgaaaataga
    36721 agactttctt gttttcaatc aaaacgctgt gaaggcctcc tttacaagtg attcagagaa
    36781 agatacttta ctacataagc tacaagaaaa ctatgacagc tatctaaaat aaccttcacg
    36841 attctttaaa aatatgatag aatagtggta atttacaagt ggaggaagat atggctttag
    36901 caaagattgt ttatgctagc atgaccggaa atactgaaga aattgctgac attgttgctg
    36961 ataaattacg tgacttgggt ctagatgttg aagtagaaga gtgtacgatg gttgatgctg
    37021 cagattttga agatgctgac attgcaattg ttgccactta tacatatggt gatggagatt
    37081 taccagatga aatcgtggac ttttatgaag atttagcaga ggttgattta tctggtaaag
    37141 tttacggcgt agtgggttca ggtgatactt tctatgatta cttctgcaaa tctgttgatg
    37201 agttcgaggc tcaattcgca ttaacaggag cacaaaaagg tgctgattgt gttaaagttg
    37261 atttagcggc agaagacgaa gacattgaaa acttagaagc ttttgctgaa gaaattgctt
    37321 ctaaacttaa ctaagctacc aaaaagacga acacgagtcg tctttttaac ttttttacca
    37381 tcttttgtaa ctagaatatg agatatagat atggaattag aagagattcg tcaagaaata
    37441 gatgaaatag atcagcaatt agtaagcctt ttagaaacta gaatgggtct aattttagag
    37501 gtcatagcat ttaaaaagaa acatcgactt ccagttttag ataataatcg tgaaaacgaa
    37561 gtgcttaaca atgtattgaa gaaagtccaa aaccatcaat ttgacgatgt gatacgagca
    37621 actttcaaag acattatgac agaatctcgt gtttatcaaa aggagaatat tgtagacggt
    37681 gattaaacga tttgataatt atgctcagta cgtacttgtt ttaggtttta gcttactaat
    37741 tggtggtgtg gttggggcta ttacagcagt ctttggacgt gttctattat tcctgacagc
    37801 ctttagaagt gattatatag cttacctact cccttttctt agcattgttg gtttatttat
    37861 agtttttgtc tatcaaaaat ttgggggtaa gagtgttaaa gggatgggat tggtatttga
    37921 agtaggtcat ggtaatgagg agactattcc gaaacgtctt gttcctctcg tgatattaac
    37981 aacttggtta acccacttat ttggtggtag tgcaggtcgt gagggggttg ctgtacagat
    38041 tggggctacc gtttcacact actttcaaaa atattgccgt ttacaaaacg cttctcaact
    38101 tttcctagta atgggaatgg cagcaggttt tgctggttta tttcagactc ctttagctgc
    38161 tacttttttt gcgattgaag ttttggtagt aggtcgttta atggtttcgt atgtattacc
    38221 aagtttaatt gctgccctga cagcaaattt cgtctcacat tctttaggtt tagaaaaatt
    38281 ttctcatagt atagcaacat caatggcgtt aacaccagat attattctaa aattattggt
    38341 tttaggacta tgttttggtc tgtgtggtaa tttatttgct tatttattgg ctaaagctaa
    38401 attaattgct tctagtaggt tgctaaaccc atataaacgg attttcacac ttggattgtt
    38461 agctactttt ttattgttta tcttccattt tggacgatat tcaggactgg gaacaaatct
    38521 aattgaggct agcttcacta ataagaacct ttatgattat gattggttgt taaaattgtg
    38581 tttgacagtt attactttgg ctgcaggtta tcagggagga gaagtaactc ccttatttgc
    38641 aatcggagca agtttaggag tgattattgc gcccatttta ggtttgccag ttattttagt
    38701 tgctgcttta ggctatacta gcgtttttgg gagtgcaacg aatactctgt taggtcctat
    38761 cttaatagga ggcgaagtct ttggctttgc caacactcct tattttgtta ttgtatgtct
    38821 tgttgcttac tccatatcac atgctcatac gatttatggt gctcaatcta gatagttagt
    38881 gatgagtttt aaaagagttt atcagtttgt agaacggaat attccgttct cttttttatt
    38941 ctcttaaagt ttataaaaat ggagtttgtc aattttatga atgcaaaaat tatgtggcta
    39001 atataatttc ttcaaagaaa ttgattttat gattgtcatt aaaatactgt aaaatccaat
    39061 tgaatgtgta ggaacttagg ttaggataat gtctacaagc atttaagtcg cgatggtgaa
    39121 atgcattata agataaaaag ttgatgtgga tttcaacttt atgggaaaga gttaagtgac
    39181 acgaaagcta atatagaaaa cgtagcttaa aaaatcaaat attaaatgaa ttaaactctc
    39241 aaaaatctga aaaaagctaa cctagaaaca gtaaatcaaa tttaaataat atacaaataa
    39301 ctattctaaa ggatgacacc ttgttcttct agataaaata gctttacaag taggttaaaa
    39361 tttggtagaa ttgtgagagt gtgtgatgga cgcacaaaaa acggctaatc cgctgagaca
    39421 agtacttaag attagtaaga gaaggagaat aaaaatgaat ccattaattc aaagtttgac
    39481 agaaggtcaa cttcgttctg atatccctga gttccgtgct ggtgatactg tacgtgttca
    39541 cgctaaagtt gtcgaaggta ctcgcgaacg tattcagatc tttgaaggtg ttgttatctc
    39601 acgtaaaggt caaggaatct cagaaatgta cacagtacgt aaaatttctg gtggtatcgg
    39661 tgtagagcgt acattcccaa ttcacactcc tcgtgttgat aaaatcgaag ttgttcgtta
    39721 tggtaaagta cgtcgtgcta aactttacta cttacgcgca ttgcaaggta aagctgcacg
    39781 tattaaagaa atccgtcgtt aattttgatg atcagatttt aaaaatgctt ggttgtttga
    39841 ggatagtaac tatgttttaa aactggacaa ccaagacgta aaaaatctgc ctgtgggcag
    39901 tttttttact aggtcccctt agttcaatgg atataacaac tccctcctaa ggagtaattg
    39961 ctggttcgat tccggcaggg gacatgtaaa taacgtcaaa agcctttgta ttaaaggctt
    40021 tttgttttat tccgatttta aaaggggcac aaaaggggca gtttgtttat ttataatttc
    40081 tttcatattt acagttgtgt gactgtaaat agataatgtt gtttttggat cgctgtgacc
    40141 aactctatcc attatcgcat ttagcggtat ccctttttct gctaaaaatg atatatgcga
    40201 gtgtctaaat aagtgcgtgt gataatctcc gtaaattttc aatcgcttat tgatgtacgc
    40261 gtttaaaatc ggtacaccgt tcgaatttgg aaagacgaat gaacttgtct ttttctgtct
    40321 attgacgata tctaaaatat tatctgatac agatattttg cgtgttgatt ttttggtctt
    40381 ggtcgtagtg atttctcgtg tgttaaaatc gtaagttgca ttaatcagaa tttctttatt
    40441 ttcaaaatct attttgtcgt aagtcagaca agctaattct ccataccgta cacccgtcaa
    40501 aaacatgaat aaaacgatgt ctgcgagcgt ttgttcgtca ttgtctatca ttctattgca
    40561 caggtcgtaa acctcgttag atgttaaata aagcaccttt tcaggcttgt aatcatcctt
    40621 tggtttggga actaagacgt tctccgtcgg attacttgtc atatagtcca tttgtatcgc
    40681 ataagaaaat atagcatgca atctctttct acatttatgc gtaacatgat acgaattgtg
    40741 ttttaatagt ttatcaatga ttagtctaat atcacgttta gtcaacttgt tgataattgt
    40801 atcatctggt aacacggttg ctatatgact gtcagatact aaataaccac gttttgtgga
    40861 gtctttgacg gtcggtatcc attgctttag atattccttt ttaagttctc cgtaagtcat
    40921 ttctgagtga tttctgatag cgagcctatc ttcaatcttt tcctgcaata ttaaaccagc
    40981 ttttttctga gcttgactag aatttttatc tagtgtcaca gatacttttt tgtacttatt
    41041 tgttagaggg tcagtatatc tttcgatata tttaaatttc ccgttggcta gctcctctat
    41101 ccacattgtt ttatacctca ttttctgtta aaataggtat ggtaaaaacc cctcccaaaa
    41161 agcaggtttt tactatacta gaatttgcct cacgctctcc ttggccaaaa tttgatcgtg
    41221 gggctttttt tattgttcta tttagtatat atgcttattg tgtcatcgtt gttaaatggc
    41281 tcaaatatag ctttaatagc ttggtaaaaa ccaatgaaaa ttggaacagt agtccatgac
    41341 aagaagaagc ataacaaacc ttttttgaat tccttcatgt agaaatattg gcccccgatg
    41401 ctgccaagga agaatgcaag aagacagtaa gctagcttgt taactctgtg ctcttttaga
    41461 aatacttcat ggtattcata ttgaatagtt tttgattttc ctgaatatga ttgattgata
    41521 ccagaaattc caccaaatgt agtctttcta tagactttat tatataaagc tttttttgga
    41581 tgtaatatac ccatgccttt tttcccatat ccaggaataa ctttcttttt aatttgtctt
    41641 tttagttttc ctgtggttcg agctttaaat gattttttta aactagattt tctcattcct
    41701 attttcatat caatccccta aatcaagcgt ggaattttta gttattattg atttttttga
    41761 gttcatccaa aatttcttgg ttttgactaa tcagtatcca attttgttcc actaatgctg
    41821 ataagtatgt aacttttgcc tgttcctcag cttttgcaaa actcaaggcc atgcctgctt
    41881 taaaaaatcc gttaccagcc aaatcattaa tcacacgtct tacttgcacg ttagattttt
    41941 cgttcaagtt gtcaattcca cgttgagata aatatttttg tattttttgt ttttgttttt
    42001 cttctctaga taatttttct ccgccaaata ttgccatagt aattctccca ttattaaatt
    42061 aaattttaaa aatcatttac agtataaaaa ctaatcattc ctataatgcg taatgcgact
    42121 caatattctt ttggcataaa gttgccgaca acttttccga taatacggaa atcactatct
    42181 ctatcgattt ggatatcact atatttatcg ttcagactat gcagaaacgc cccctcggcg
    42241 tgtataagta gctgtttgat ataagcgtca ccgtaatatt caaaaacgcc tatatcgcca
    42301 tctacgagct ctacagataa ttttacaaat acgtagtccc cagagtgata ttcgggttcc
    42361 atagaatcac catagactgg aataacaaaa tcagcgtcat aatcgactgg caattcaatt
    42421 gtttctactt gtacatcatt tagatactgc cctgtaccag cagaagcagc atggtcgtag
    42481 tagttgtaag agaataattc tactactgta ttcttactat cttctactgt gttttgttca
    42541 aataaaagat tttcaccgta acttatccaa tcactatgac gaggttcttt gagctcttca
    42601 tctagtagag cgactttgtt gttgatatta ggagcagtag aagttttgct tgatttaaaa
    42661 cgagggtcga tatctgattt taatactccg aaaaaatcag ctattttttg aacgttacca
    42721 ggtattggca aggatgttcc ttttatatat cctgtcaagg tgctaggcgg aatgcccgtt
    42781 cctctagaca catcaatctg cttcctgttt tgagtaatca agaagtgatt tatgttgtta
    42841 gaaattattt tcatatactc taaatcttga ggagttaact ttcctctgcc tctagccatt
    42901 aatttatcct cctgttgaac atctacctta attatagcgt attaaatcgt attttgaaat
    42961 aaaaaaatat aaaaaattac gatttttttc gaaaaacagt tgactttcga attaattcgt
    43021 attataatta aatcaagctt aaggaattaa gcgaaacgaa aggaggtaca gctaatgaaa
    43081 tctaggctaa acaaaaagcc taaacacaaa gaagtcgagt tggaaattca cattctttgg
    43141 tttaagctca aaatcaaata tctgattaca tggtaatcgg ataggggggt gaaattcccc
    43201 cacccctaat ggggtaagtt tagtttagca cattggctgt atctctgcaa gaatgaaagg
    43261 agagcagatg gaagaaaata acatttataa ttttcatgca gtacttggaa ttatgctacg
    43321 taatattcaa gaagattaca tggaaataaa aaaccctcta gcaaggtgtg agcttgctaa
    43381 aggatacttt gagattggta gatatcttta cgacgaaggt gtattacctt ccgaatacct
    43441 tggtgagagc atctctagac atcttagctg attccttttc ggcttcaata aaaggtaaat
    43501 tatgtttctt aatagcttcc atagagtctt cgtaaagttt gattttatct tcgatagaca
    43561 aagtagggct agaagaagcg acaatagcga gagctaaatc ttttgaatta gaaattttca
    43621 ttagcttatc ctcctttctg ctaggataag ttgattataa catttttagg aggtacaaaa
    43681 tgaattggaa aaaactaatg tttggcgatc tagaacacac gtttactagt cgtaatggca
    43741 aagaaaaaac aagtattgaa tttgaaggtg gcgtattgcc agcactgtta gtgctaggtg
    43801 gtatcacttg gctgatcgct tggtttatta caaaataaaa actcccaagt gggagtggaa
    43861 aggagagggg tcagaaaaat gattaatctt gaagaaaaag ttcaaaaaat agaacagaac
    43921 gaagaagaaa atacaacctc aatctcattc ttaagaggtt atatcaaagg ccttgaggag
    43981 cgaattagtc gtttagagcg ttcgaagggc tgttaaaggc attagggtat tttgtttgta
    44041 actcaaaaat cttttctaac ttttctggct cttgaaaagc catcataatt aagtcttcct
    44101 gagtcattgg tcttttttga gtagaattat tttctatttt atcagataaa ttttcaattg
    44161 catcatagat gttgttaatt gaagttttta attggataat atcttgcact gaagcacttt
    44221 ggtcgtttgg ttcatggatt atagaaaaat caatgttttc ttctgttttt tgaattcgtc
    44281 taattgtttc ttggacagaa tcaagcacaa ccttactttc agagtttttg agatcgtaat
    44341 gaatggtatt tatagttcta atatcaaaag ggatatcttc atcagtcttt tgtctaagga
    44401 aaatagtagg taagttcaac gcttttctaa acccaagttc taaaaagaca ttcgggttat
    44461 ttcctgttat atcaacaatt accaagtctg ctgtggataa agcatcaaat attttatcgt
    44521 caatcctatc tgaatgatac agttcgtcag ctctggtcac ttgatacttt tcgatcaatg
    44581 caggtttaat tatataattt aaaacactgt ctgagtggtt ccgaatttcg ctactttcct
    44641 caccgatagc agatactacg aaacaagatt ttttactcat aataatacct cacaattttt
    44701 atttaaatta taccacagaa agaaggtgga atagatgcgc cccaaaaaat atccgtatag
    44761 ccaaaaaaac tatccagcgc cgaagcttca taacatcatt gaaaccgata atcatttctt
    44821 gatagatgat aaaaggattc attatgttat tgaagattct gtaaagacaa aaacccttgg
    44881 cgatggctat gttgaagtaa cactttccat aattgccaag agttttacaa aatcacaagg
    44941 ttagtttggc gatagtcctt gtgatagaag ttggtgtaag agggatacct tcttctttaa
    45001 ggatattctt taaacggctc gaaaaattat tatctttaag actttgaaga tataagtaac
    45061 cagttgttgt tacgccatcg aaaaaataga aagtaatatc tttacattca cctcctttct
    45121 gctcacatta tagcagatta gaggtactaa aaacagatag aaaggaggtg ggggaatgca
    45181 acaaatttcg ctaagagcac tacgagtaaa ttataattta tcagctaaag aagttgccaa
    45241 aactttagaa attcattatc aaactttgtt gaaatatgaa aatgatagca gtaaaattcc
    45301 tcatgatttg ttaaaaaaat tagctgattt ttataacgtt gatacgaatt atattttttt
    45361 aggcaaaaaa tacgaattaa ttcaaaacta acacaccgct agaaaggact acatgaagga
    45421 actagcatta tcaaataact tgcaacagat tgaactagat ttaagacaag aaaacgaaca
    45481 gattggaaag tctatttgga aaataggttg catgttaaaa catgttaaag aaaatgattt
    45541 gacacatggt caatttatgg attggtacaa aaatcttggg tacaacaaga atttcgttag
    45601 caaggcaata acgatagctg acaaactttc aaatttcccg acgttgggaa atatcggaac
    45661 agaagctctc taccttattg ccaccctacc agataaccaa aagcaggaac agattgaacg
    45721 gattgaaagt ggcgataacc caactgtcag agagttgcaa gaaatcaagc gagaaaacaa
    45781 ccgcttaaaa tcagaaaatg cccgtttaga gcaacaaaaa gagaatttag cagagcaagc
    45841 cttgagtgct aaaatcgttg aaaaagaagt tatcaaagaa gttattccag atgattatga
    45901 aagcactaag caacttaatc agactttgct tggcaagaat aaagaactaa gtaagatggt
    45961 tgatgatgcc ttgcagcacg aggagtattt aaaaagtcaa cttaaagaat tctatgctaa
    46021 gcgtgatgag gtcaatcata aatcagctaa gtatgatgag ctaacagaag cgattaaaca
    46081 gtcagaaggt aaactcaata gctatcaaaa gaagattgca tcatacaaga atatcactga
    46141 actactcaaa aaaggcgatt tgctattact tgaaatgagc ggacttatct atgctgatga
    46201 aacgcactat atccagcgtg acgggcttat taagcaagag ttcgatagct tggtcgatag
    46261 aggtctaaag ctctttaatg accttgatat gaagcgtaag aacactgaaa ttttagaagg
    46321 agaagtctta tgaatgaact aacaacaact caacaactga ttgaaatgtc aaaaatgcag
    46381 acagtgactt tagaaaaggt tgataactta gaaaaaggat tattgcaagc gcagaatgat
    46441 attcaagaaa tcatggatac atcttattta catcctggaa ttattaatat gattactaaa
    46501 aaacgtcgta aacgtgtcat tgattgtatg ggcggaaaat ctgcaaaagc ttataaaacc
    46561 tttaaagtag acgaggaagg cagaaagcat cgtttttcaa gtgaagtatt ccgagaaatg
    46621 gaacttgatt tcaaagcaga atttgatctc aatagctacg ctgaattgtc taaatctaag
    46681 aaagaagagg cgcttgaata tatcgctatg tgggaaccgt gcacgaatac taaacgaaaa
    46741 attaatctgt tgaacaaaca gactgaactt gaactgattg ggtaagaaag gaacaaaact
    46801 aacgaaagga gaaaatatgg aagaaaatat caagcagttg atcactgaaa atatcttaga
    46861 atttttaggt tctgactatg gaaaaggttt cttaaccggc ataaaaattg ccgttgagat
    46921 tattgccaaa caaataccga caccttcaga agaggatatc ggtcatgata gttaattttt
    46981 gcgctgctgt tcgaaagctt gttttaaatg agttgccatc atttgctgaa cagcaagaac
    47041 gaaatccata ctatggagcg ttcatacaaa atcaaagtgt atatcactaa ggagaaagaa
    47101 catgattact attttaaaag aaataaacca aacactaaaa gaaatcctag cagaattaaa
    47161 agaacctact gtggtaacag tagattctga aaagttaagt aatacgctaa ctactgaaca
    47221 gaagatacgc cgattatctg gtcaataaac agaacaacaa atggaaggtt caaagtagat
    47281 tcttttgatg ttgacaaagt agcatcaaca agcaacataa atctttcgtt gtcatcttta
    47341 ttttctttac gatattccaa gaattcaagg acatgagcat acttggcttt atcaggattt
    47401 tcaggtaata aagttccagt gaatattcca ctcgctgtaa tcacattaag cttacaattg
    47461 cttttttccg aaaaaatagc gacatctgca ataagattag tttttaaaac gtcgttcatt
    47521 ataatcacct cctttcgaga tgattatacc actaaaaaag tccgacggga atcggactca
    47581 aaacaaactt aatttactta attatatcac agaaaggaaa caatatgcta gcaaaactta
    47641 aaagcggtat cgaagtacct tacgaagagc tttggcttaa tgataacgac ttatccgaat
    47701 ttattggaaa gtcatttgac caaacgcagc gattactaag aaagatgtac aaagacagaa
    47761 attatcgcaa atacattgac aaggttggcg gtcgttcaac aaaagttaaa aaatttgaag
    47821 aatggagaaa attacaaaat gaaaaaatta tttaacttta tttttacaaa accaaaaaaa
    47881 gaagaaccaa aaccaaaaag gacaattgaa acacacggct gggaagttgg tgcgaaagct
    47941 tatgatgatt ttcataatta tatgaaaggt ttgaaacatg gatgaatgga atccaatttt
    48001 aattaggaac tagatggttt gcctggtaag caggttttgt aggaaataca tagtcctttg
    48061 acaactgaat atgggtgcgt tggagtaaaa tatattttac aaatttccgt tttaatacgg
    48121 ttaaagtgta ttttttcttg atatttcgtt tttttcgtag tatactttag gggaagtgaa
    48181 gaatatttag cacatttagt gccaaaaaga aatcccctag taccgcaaat actaggggat
    48241 ttttctagtt tcctagaaga cactaatcat cgttgtctag ccacttttgg actagcaaca
    48301 agacgatgcc gaccaataat ggtgcgatga ttgttgtgaa gaatatttcg cacatggttc
    48361 tcacctccaa tctaaggcgg tgtagtagtg ccgagtagta ttatagcatt taaagcttct
    48421 aaaagctacg agatttcaat cctgatagct tctgatgaca agaacggtaa aatgtatgtc
    48481 tagataacag actgtctcag aagtgatttt agggctgttt tgagagaaag aaaaatattt
    48541 aaaaatgttt aaacaaaacc attgacattt gtttaaacat aagttataat taaatcatgg
    48601 ttaaggaatt aactaaatcc aacggaaagg aaacggagct atgcgaagtc gaaaaactaa
    48661 gcaaaaagaa aagctctcgc tgactgataa atttgtgatt atcggcataa tcatagaaat
    48721 aatcagattc atacgagaac ttctctaaaa gcaacgaaac cagagggcga aagccctcgg
    48781 tggtttcgtg tacttcgatt ataacatagc tcctacaggc tagcaaatga agcacgaaaa
    48841 aagaactgaa attttgttag ggattttaat tgtcttgcaa ttgttaaatc ttgccttgag
    48901 ttttcgatag gtgacagatt tggcaaaaat ggggcgacct actagcaatc caagggataa
    48961 gtatatcggt gtcagagctt caaaaaatga ggtagaaatg cttgattttt gtaccgaaaa
    49021 gactggaaaa agcaaaaccg atatcctcat ggaagggctc gaaaaagtct ataatgagct
    49081 aaaagggtaa acaaaaaaac accacgtaac tactcaaacg ccaatccgat tagttacgca
    49141 gtgccaaacg cacccatatt caagaagaat acaggatacg cttatattat aacagcgtac
    49201 ctgtatttgt gcaactcaaa tttataggta cgctttttgt gtgcctcaaa ttcgcaggag
    49261 gactgtatga aaacttacac attaactgaa gaagaattga atgaattagt agccgagcgc
    49321 atgaaacaag cgaaagaaaa acgcacacca caggggctat ttaaagatgt cggctttgat
    49381 gatgagttaa ttccgatcaa caataaatac ccaaaagtac tcaagaaatt aaatcgtgaa
    49441 cgtgcttata aaccagaaaa acacgtcttc aatcagacac caaaagtttt tggtgtggac
    49501 aacgagatta gttatagcaa aattacaaca catgacgtgc acaaccatat tcgtttgctt
    49561 gtcttaaatg tctttggtaa aagtcaaaat aaggaagtat tgcctgagga atacgaccaa
    49621 gcaatagaac tttacaatca attaaaagag tggtttgtgt ctagctatga taagcgatta
    49681 gagggattgg tactagaaga tgattaaaaa aatatgcgtt aactacttac taaaacagat
    49741 tgacaaaagc aaactagaaa caagagataa agcgaagttg aactacttta tcacactagt
    49801 ggactgcaag ttaggaggat aaattgggta aagaaaaaac taaaatctac ttttggctaa
    49861 agttcgacaa gaagtttttt gaaaatattt ttatcaaacg tttaaaaaag atggctggcg
    49921 gagacactat gacagtcttg tatattcgtt tgatgctaga aagtttagaa acagactgca
    49981 ttttatatta cgaaggatac tttgataatt tggtagaaga gcttgccatc aagttagagg
    50041 tgtctgaaga cgatatcaat atgacattag cttacttcac aaaatgcggt ctaatccaga
    50101 tagacggtag cggtaacgca caaatgcccc aagctaaagc tatgctggaa agcgagacga
    50161 actgggcaaa atataaacga gaaactagaa aaattggaca aattccaacc gatgtccaac
    50221 caatgtccaa ccaatgtcca acagagatag agatagatat agagaaaaag aaagataaag
    50281 agagagagtt agataaagag aaagaatata ttgtcgagca gagcccgact gaatatctct
    50341 ttccagactg gttagaagag aaatatgtcg aacaagtcaa aaaaggtaat cccaaaaatt
    50401 ttgattatcg tatcccaata gcttatctca accaaaaaat gaactctaac tataagtttg
    50461 taaaaacaaa cacagattta gtcaaagcga gactaaaaga tagttatact ttagaagatt
    50521 tcaaagctgt catagataaa aaatgcagcg agtgggtaaa ttctgacatg gaaaaatatc
    50581 tcaggccatc aaccttgttt aatgctagca agtttgagag ctatctcaat cagccagaag
    50641 ttgctaaaag tgattattac cagaagcaac aaggccaacg attttcgcaa gctgagttag
    50701 atgagcttaa gaaaccagat ccgaaatatg gattttagga ggtatctatg gcttttgggt
    50761 taatgacaag agagagcatg ctcgagaatg gcattattag agatactggg aaaacatgcg
    50821 aaaagcacga gatgccaatt tatgctagga aaatgccaaa tcatggcaat agagaaacag
    50881 aattttgttg gcaatgtaca acagagtata tccaaacgaa aagtaatgcg gttgacattg
    50941 cgtacaacaa ccagtcgttg ctagctaagg gttataaagt gttttataaa gagagcgttt
    51001 tatcaaagga aattgctagt gctacgttga aaaactacaa ggaacatagt gctgtagata
    51061 caaaagcgct aaactatgcc aaacgaatca ccagagatta tgttaaagga atggaaggta
    51121 actccctctt acaaggacct ccaggggttg gcaagagcca cttgtctatg agtattgcta
    51181 aaaatattaa cgagatgttt aaatcttaca atcaatcaaa gagtgtgata tttgtttcgg
    51241 tacctttgtt gtccggacta gtcaaagata cattcgatta tgacgataaa aaaaatagca
    51301 aatattcgca agaaagaatg tcaaagcttc tcatcaattg tgattatctg atacttgatg
    51361 acttaggcaa ggagtcaacc acaggtaaca ccattaaatc tgctagcggt tggacatata
    51421 cgtttttatt taatattttg gataatcgga caaatactat cattaataca aattttagta
    51481 gagctgagct tatgaaaatc tacgatgctg cttttgtcga tcgcataatc aaaggtgcaa
    51541 aaaacaatat ttttaaatat ccagataatg cagaaagtaa gaggttctga tggaactaac
    51601 attaacaaca tttttcagct tgtcagaaga acatgcagca aaaatcatgg ctctagacga
    51661 acctagtcga aataaaaaaa ttgaagaata taggcagtta agactgcgca gagggaggat
    51721 tgactttgga aaatagacca gatttaaaat tagtagctga attagaaaat aggattaaag
    51781 acttaaaaat tgaaaatgaa atcttaaagt ctaaaaacat tgatttgtct gaagatgtta
    51841 aacatttaaa atataaatgc ttcgaaaaag atagttctat agtggatatt atggttaata
    51901 ataacgacct aagaaaagaa aacaatgagt tgcgagaaat gtttgacttt ttcaaagata
    51961 gactagagaa atttgtaggt agttcgcatg gtagaaatta ggattaatgg cgaacttgta
    52021 acgtttgacg gtaattttag ggatgctttt atatttacaa ttgactattt acgagacagc
    52081 gaagagccta cgctaaggca gacttacaac gaatttaaag actatacaga cgaagacttg
    52141 atggaataca ttgaaacgga atttgatgta aaacctgaat taattgtcaa tcggagactt
    52201 gatagtaaat gggcttttaa atctcacatt ttggaagact aaatatgagc gaagagttat
    52261 acgagtctac tcgttattgg caaagtagat acagcgactt gatgtctgat tatcttaaag
    52321 aagctgaaga aaatatagag ttaaagaaac agttgaagag attaaaagcc gaaaattggc
    52381 aattgaaaca tagaaagagg aaataaatgg cttatttata cgagttacag ggtatttatg
    52441 cgcaattatc agctatggat cttgatgatg aaacatttca agacactttg gatagcatcg
    52501 attttcaatc agatttagag aataatattg aatattttgt aaagatgtta aaaaatacac
    52561 aagctgatat tgaaatgtac aaaaacgaaa aagaaacttt ttacaaaaag caaaagcaag
    52621 cagaagccaa agcggaaaaa tacaaagata caattaggct agcaatggac ttaagtcaaa
    52681 agaaaaaagt tgatgctgga atgtttaaag tatctttgcg aaaaagtaag aaggttgagg
    52741 ttttggacga aacaaaaata ccttttgaat acatgcaaga aaaagttgaa tacaaaccaa
    52801 aaaaagatga aatctcaaaa gttttaaaat ctggaattga tatatctgga gttcaactaa
    52861 tcgaaacaga aagtttacag gtgaagtaga tgagcatgac ttttgcagaa ttgcagacaa
    52921 aaatgcaaat aacaaaaaca acaaaacaag gcgttaaata tacatttcgc aatgcagaag
    52981 atatttttac acactttaaa acactaaata gcgggtggga gttaacggta tctgacgaat
    53041 tggtggaatt gatcggcaga atttttatca aagcaacagc aacagctaga cttggtgatg
    53101 aacaacacca agcgacaaga tatgctgagt tggacagtgt gcctgtttta aatactaaag
    53161 actataaaac aggagaacct aaacaaatac aacaaatgca agttccgcaa tggactggtg
    53221 cagtgagttc gtacgcaggt aagtatgcct tgcaagggct gtttggaatt ggtgaggaag
    53281 atgtagatgc gattgttaca gaagatacgc aacgcaaaga acaaaaaacc tcccgaccga
    53341 caacctctaa aactcctaaa ataagcaata tccaagtcga gacttacaag tctgatttaa
    53401 atgatattgc gaaagccaca aaccaaaacg ttgaagagtt aacaaaatgg ctaaccgata
    53461 ctttaaaagt ggggacactg gaaaatttgc atacggaaca cattgtttcg gcagacgaat
    53521 taatcaataa actcaaaaag aaagcaggac taaaaaatga ttaataatat tgtacttgta
    53581 ggtcgcatga ccaaggatgc cgaacttcgt cacacgccaa gtcaagtagc tgtagctacg
    53641 tttacacttg cagttaaccg cagattcaaa gagcaaaacg gagagcgaga gacggacttc
    53701 attaattgtg ttatctggcg acaatctgct gaaaatctag caaactgggc taaaaaaggg
    53761 actttaatcg gtatcacagg gcacattcag acacgcaatt acgaaaatca gcaagggcaa
    53821 cgtatctatg taacagaagt tgttgcggaa aatttccaat tattagaaag tcgcaatagc
    53881 caacaacaga ctaatcaaag cggcaatagt tctaattctc attttggcaa tgccaacaaa
    53941 atggatattt cagatgatga cttaccattc taaatatgac gaagccgcgg aagcaaagga
    54001 tatatgcaat atatgacgac gacaagtttg tcgacgttgg cacaaaagaa gagttatcag
    54061 cacggcttgg tatcaaaaaa gcaaccatag aacagtacat gactaaatct tatcaagcta
    54121 gacctagctc aaaaagaatc gctatttttg taggagttga agaaattgaa ttttaaaaca
    54181 gagtttgaga taccagtaga gccaaaacca caaacaagac caaaatttag caagtgggga
    54241 acgtatgaag accctaaaat gaaaaagtgg agaaaacaag tcactggttg gattgaaaaa
    54301 aactataatg ggccattttt tgataactgt gtaaaagtag atgtcacgtt ttacatgaga
    54361 gcgccacaaa cgctaataaa agagcctacg gcacgctcaa aaagtaaaac cgtacaaata
    54421 tatcaaaaat ttataaacga gcttatatgg cacgtaaaga agcctgatat tgataaccta
    54481 gttaaagctg tttttgatag tatttcagac gcgggttatg acaaaataca aaaatcgggg
    54541 attgtctggt cagacgataa tattgtatgt gacttaagag caacaaaaaa gtatagtcca
    54601 aaccctagaa taaaagtaaa aattgaggaa atagatgaac gaactaacgg ataaatttta
    54661 tagtatcttt gatagcagta ttttgagacg tgtcaaagag ttaaatctag atgataaaac
    54721 atcagaacgc ttaagactaa atatctcaaa taacaagcgt agaaatatac tgccaaggcc
    54781 ttacgtaatc gaagcattta aagattattt tgataaagac acttatgtgc agctgtatct
    54841 taaatcatac agagagtatc acaacccaaa cagccatgaa actgatattt ttataaagtt
    54901 aaacaaaaag cacagagata caaagttaga ccattacaag aaagttaagc gattgatgta
    54961 cgcagcaatg actttctgag gaggtatagt atggcagata aaataaacgc agagagtatg
    55021 caagctgcat acaacgaaaa ttatcaaatg tttttggcta aaaatgcaga ttatgggaac
    55081 tcgttcgaaa agtctttgaa tgacttcgga tttatcgctg gtgtcgtccg tataggcgat
    55141 aaatacaaca gactatataa tcttataagc agcgacaaaa acgtctcaga aagcctgtca
    55201 gacacgttaa acgacatggc taattattgc acaatgttgt caatctggct agagaaaacg
    55261 gagaatgcaa atgacacacg tagttagggt ttacgatcac attggtggac gagtgttgcc
    55321 tattactaga agggttagaa ggatgacaaa atatattgaa attaaagatg attggaaaaa
    55381 agcagtagac catttaaatg attttatcga aaagtatagc tataagaaga ttgttgtcgt
    55441 tgggtatcag gttgttagaa accccgagac aaacggaaca tgtactcatg tgttagttaa
    55501 agtggattga tgaaaaaaat atcaaattcg aggtaacaga atgacaaaag aagaagtaat
    55561 tgcatttctg acagaacagc gtgatttgcg gcttgttgca tatgagtggg gaaaagacaa
    55621 tctgtccgtt tttgcgagat ggcaattaga acaagcaaat atgtatttag atatcattga
    55681 gtggatagag gaggtgacgg aatgactgaa caacagatga ttgattgctt gctttatgag
    55741 ttagcaaaaa aagacaaatt gaacattaga cgaaacaata tcataacgtt tttatcgatt
    55801 gtgctgatgg ctatatctat tttaaacgtc gcactgcaag accactacaa gctacaaatt
    55861 acaggattac ggacacagct aagcagaaca caaaagcaac ttaaacgtgc tagcgagcaa
    55921 aatcagagac agactaagcg gattgcggaa cttacaggaa atgggggata gggtatgatt
    55981 gacgaaattt taaaaagact taataaagaa tttgacaatg atctggataa ttacgaacaa
    56041 gaaagatacg ctggttacat ggacgcaata ggtgtagcaa ttgaaattgt tgaagaagtt
    56101 aagcgaggta gaaaatgaat attgaagaag cgaagaaatt gatagacaaa cagtctattg
    56161 gtaaaggtgg tgtcggcgac attccagtag tgaaaacaca tattgtaaaa gtattactcg
    56221 accaactcga caaaccaaaa ctagaagtac cacaaatgat agctgatgtt attgaaagct
    56281 ttgacgaaga tgtgaattat ttgcacgaac atatgagtta tcagtctgat gaagttagag
    56341 agtggctaac tcacaatgaa cgtgagtttt atgaagcttg gctagcttat ccaaatatca
    56401 cagtcgaaaa agaaaatctg tatactgttg agatacctaa tccgaatgaa agacagttaa
    56461 gttttgtgct gatgagacag cttagcggaa atgtaagtat caaagttatg catagagata
    56521 acttagactt actaaagata gataacaatt tacaactcac agaatccgaa atccgcaaag
    56581 attttgactg ggcttggcag tttagaaaag atgtagaaaa tgaatgaaag agagttaaat
    56641 aaaaattatg aattatgata aaaataaaaa tgatgctaaa aagaacttta ttatagcttt
    56701 agttctattg ccgttcggct tagtattatc tggatttgtt attaaatacg gttggaataa
    56761 cattttatca acaattgatg gcgtaccatc tatcaattta ccgcaagctg taggaatcaa
    56821 tgtgttaatt agtccttttg cttctaaaaa aaatacagat gaagattttg ctacagtgat
    56881 tgcaagagcg tttatttctc cgctagtcgt attgttattg ctttggatag tgactttgtt
    56941 tatgtaatat cttgaggtaa cagaatgaat gaagaattag gagtgttggt aaatcccaaa
    57001 cgtgcgtatt atgtaggaaa taatcgggac ggattaccag tatacacagt caacaccgaa
    57061 tatgcacaca aatgcacacg caaagaagca gaacagtttc cgcagtttag atgggtgagt
    57121 ttggaggagt tgagatgaca tatttaaaaa aattatttga agccgttaaa tttctcaact
    57181 ctatggaaaa acagaacaaa aaaataatga ttactttcca taataaaaaa gttcattttt
    57241 acgagttaga agaaattaca gagtcttcga gtggtactgt tgaagctaaa gcgaaaatgc
    57301 ttagtttatg gggaaagatt aaattatgaa agaaaaaaca atttttatat caaaaaaata
    57361 tgcaaatgac tttaacaatg acaaatataa tttgtcctct ggctattatt ttagaagtgg
    57421 tgaaaaacat gatattgcta ttgttaaata tggtgaaaaa gattatttaa aaaatactga
    57481 tttagcatat gttgtatgcg ataagatcgt tgacgcagac tctataagct tcgtttatca
    57541 tggtgaatat gaaacttggc attttaaact attaaacaca gaagcaaatt aaagtcccac
    57601 gcaagcgcct aagagcctgc aatagctctg tgggtctacg agctggaata ctcgttaaac
    57661 ttaccctgga agctttctgt aagtattcag ctgcgtagcg tggaataatc gttacgtagt
    57721 tatagagcga aatttttaga aagggaaata tcctccgaca tttttttcat aaaaatctaa
    57781 agtctgttat cgctcacaga tgattataca agcgtaatgc tgcaaataaa gtgctgacgc
    57841 aaaactaaaa atttaatact cgacatttaa caacacaaaa aaacagtcag cagaaaaagg
    57901 aaaggagaac aataaaaaag cgctcgtgaa agcgccattc ggtatatatt cgtacaacta
    57961 ttatatcata cgaggagctt tcatgacgtt ttttccagaa attaatatac aaaagactaa
    58021 atcaaacgct aagcgaaaac taagagagta tccacgctgg cgtaggatcg ctaatgatgt
    58081 agatactcaa aaagtgacag ccacttattc ctttgagcct agacaatcac acggaactcc
    58141 tagtaagcca gttgaacgct tagcactcaa ccgtgtgtca gctgaacaag aattagaagc
    58201 aattgagcaa tcagttagta tgatattgga gccagaaaag cgtaggattt tgtatgacaa
    58261 atacctatct ccttacaaaa atgcagataa ggttatttat acagaattat gtatgtcaga
    58321 gagtttttat tatgacacgc tagatgctgc attattagct tttgcagagc tttataggga
    58381 gggttctttg attgtagagc aaggagtttt tgactagttt ttatacagta atacaatagt
    58441 ttatacataa aaatatgtgt taatatagta ttatcaaaat agcaagaaga gataatcatt
    58501 taccaacagg ctatttattt agtcgtcaac tttaactact atcgaacttg ctattttgta
    58561 gctaaaaggc gagatagggt gttgagacgt agctcagttg ggggagcgat atgactataa
    58621 agggtctgga acgtacgcag gttcgaatcc tgccgtctca atagtggtta tttcagccac
    58681 tagagcaata cagcgggcgt gggacatgga tcggagtttt aaccgttttt ttgtgtagac
    58741 ctagtggtat taatcacgtt cgattcgtga tgggtctata ggcttacttt aaaaataagc
    58801 actggtatct ctacggggac ctttgcgcca agtaagacta aaccgttgga acatgaaccg
    58861 tgattggaaa acggtagagg tagcgccttg ataattggat tgtcgacggt ctgattatat
    58921 gtgtcggttc gattccgact gttcctatag cgtgtattgg caacaaggtt caattccttg
    58981 tgatgtaagt agctaaatat ctaggcgaag tggttcgaat ccactcacac gctttactcg
    59041 gtcatcacat tgtggtggct ttttattgtg gagaaaaaaa tgcttaaatt agacgaaaaa
    59101 aaaataagaa aaggtaagcc atttggactt ccgtaccaag gtagcaaaaa gaaaatcagc
    59161 aaaaagatag ttgaaataat caagcaaaat tttggcacag acaaaaaaat atatgacatt
    59221 tttggtggag gaggtgcaat aactgcagag tgtatgctaa ataatttaga agtgcattac
    59281 aacgatttaa acaatgatgt tactgatatg tttaaaagag ttattactca agatagagag
    59341 tggttaaaaa cactaatcat cagccgagaa gaatttttaa aaataaaaac caaaacaaac
    59401 aaaacagttg acgacaatat taaattgctt gttaacagtt ttgggaacaa ctcaataagt
    59461 tttttgtata acatagaatt gtcagatata aaatataaat tagcggttga gattattaat
    59521 aagcatgatg tatttagtgg ctataagcaa acagacacat acaaagagac ttgtctttta
    59581 tgcgaggaag aaaagcttgg taaaatcgga aacttacagc gattacagca actagagcaa
    59641 ttgcaacgat tacaacagtt agaacgatta caacagttag aacagttaga acgattagaa
    59701 gcgacaaatt tagattataa agcattctcg aatgttgatg gcgctatttt ttatcttgat
    59761 ccaccgtatg aaatgacgag ccagcattct tacattggtg atacatttga cagtcaatta
    59821 ttctacgact gggcgtacga aatgagcaag gaaaatattg ttattgtttc tagttacgaa
    59881 atatcagatg atagatttaa gtgtgtgtat gagtttaaga cagctagaag cacaatgcaa
    59941 ggaggaagtg cttgtggtaa aaccgaaaag ttgtttatgg tagtgtagct gactgttgag
    60001 tctattttta ttatgcaaaa aagaaccaca acagcggctc ttatgcttgt aattttaatt
    60061 caagtgcttc agtaaggact tgagaaaagt tgaggttttt atcttcggct gcgttgttca
    60121 accactcagg aatagtcacg tttttgcgta ccttcttaga gtgatatttt ttcatgtagg
    60181 caatcatatc aatgccaatt aaagcaatat cagaatcagg atactgttct tttaaatcag
    60241 aaacggagct agcctttgga taatcagtat agtcttcaag agcaaatcct aagacttcga
    60301 cagccatttc ataggcgtgc tttaagtcgt tgccgaaagt aatagcttca ggaacatctg
    60361 gaaaacgaac gccaattgaa ttagtttcgt tgtcgtgtgt aaaaacagct ggatagatta
    60421 acatagtttt tcctttctag gtaaacaata accaagatgg tcagagctta tttcagccct
    60481 gccttcttga gaatatcgtc ttcggtgcct tttggcatat cacctttgtg catgggcacg
    60541 attgtgatgt tgcctgtttc aaagtttttg tactttgcgt gactaccgtt ttgactgact
    60601 tttacaaagc cgttcttttt caagagtttg atgatttctc ttgcggttct tggcatattg
    60661 cttacctcac tttctatact tatattatac acacataagc aataattgtc aagcaaaaca
    60721 cgcataaaat acttattttt ttaaaacgga ggaggtggtg gaaaataggt aaattaacac
    60781 taaaacaaca aaaatttata gatgagtaca tcatctgcgg gaatgcgact gatgcagcga
    60841 taaaagcggg gtatagtaaa aaaaccgctg gtcaaattgg cgagcaaaac ttgaaaaaac
    60901 ttgaaataaa gcaagctata cagagtcgta tgaaagtctt agaaaagagt tcgatagcaa
    60961 cagctgatga agttcttaga gtgtttacac aaattcttag acaggaatta actgaagaag
    61021 taacggaact taatcagatt actggtgaat ttgtaacgat agagaagcaa ccatcaatag
    61081 cagaggttat aaaagcagga agcgaattga tgaaacgtta cccaacaaac ctagaactca
    61141 aaaagatcaa ccttgaaatt gagaagttga agtctcaaat tggcggagat gaaggacaag
    61201 atgagaaaat agccaatttc ttgaatatgg tcaaaggagc tatttcagat ggagttgagt
    61261 aagttataca caagaaagca attgcaagtt cttgattaca tttggaataa cgattggttt
    61321 atctgcggac ttcatggagc aaagcgtgct ggaaaaactg ttgttaacaa cgatactttt
    61381 gtaacagagt tatatcgtgt tcgtaagatt gcagatgact taaaaataga tgaacctatc
    61441 tatattttag ctgggacatc atctacttct atccaaaaca acgtgttaca ggagctttat
    61501 aataaatatg gctttgagcc taaatatgac aaacatggat cttttacatt ttgtggcgtt
    61561 aaggttgtgc aagtatatac ggggtctatt agtgggttaa aaagagctag aggttttaca
    61621 tcatttggtg catatgttaa cgaagcttca cttgctaacg agacagtgtt caaagaaatc
    61681 atttctaggt gttctggtga gggtgcaagg atagtttggg atagtaaccc agataaccct
    61741 aatcattggc tcaaaacgga ttatatcgac tctgacgatg atatgattat tgattttagt
    61801 tttaagttag atgataacac ttttctatct aagcgttaca ttgagtcgat aaaaagcgca
    61861 acgcctagtg gaaaattcta tgaccgtgac attttaggta aatggacggt cgcagagggt
    61921 gctatttatt ctgattatga taaaaacgtc cacgaagtcg aagaattgcc aaaaatgaca
    61981 cgcttttttg ccggtgtcga ctggggatat gatcactatg gttcaattgt gattatcgga
    62041 gaggacaata atggaaatta ttacttagtt gatggtattg cagagcaata taaagaaata
    62101 gattggtggg taactagagc caaagagttt atacaaatct atgggaatgt tacattctgg
    62161 gcagatagtg cgagaccaga acacgttgct cgctttaaaa gagagcgtat taaagcaaga
    62221 aatggacgta aagaagttgt tgcaggcatt gaatatatag cgaaatcatt caaggataaa
    62281 aggttattta tcaagcgagg gtgtattcct cgtttttttg atgagatata tcaatacaaa
    62341 tggaaaccta acagcacaaa agacgaacca caaaaagaat acgatgatgt gctagatgcc
    62401 atcagatatg ctctttattc acagcataaa gaagatagca taagtaaaac caacaatttc
    62461 aatgtacttt atcaaggtct taaaaactag ataggagaaa caatggcaca catagaagat
    62521 tttattgact caactgggga acataaactt ttagagttgc gttttcatcg tgagtcaaga
    62581 atgaggtatc agataagcga tataagcgct ttgtttgatg acaactataa aatattactt
    62641 gaatacttaa aacaccacga gaacattcaa aaaccacgta tacaagagtt gttagattat
    62701 gcagaaggaa acaatcacga ggtatctaag tctggtcgta ggcaagatga ggacatggct
    62761 gatgttcgtg ccatacataa ttatggcaag tatatatcaa catttaaaca gggatatctt
    62821 gttggtaatc caatacgtgt tgagtatgac gatgaagtta acaacgaact tttaaaagag
    62881 ttagcaaaaa agaacaattt ccaccaatta aatagacaac ttataaaaga tttgtctaaa
    62941 gttggtcgtg cttacgaact cgcttatcgt agtgcagatg ataaaacaaa agtgataaga
    63001 ctagacccga gagagacttt tattatctat aaaaatgacg ttgataaaga tagtcttatt
    63061 ggtgtgcgat actacaacaa aagtcaaata gataaaacag acaagacggt agaagtttat
    63121 acaagttcgg aagttatctt ttttgaattt aatggagaat taacagagac agatagacaa
    63181 ccgcacgctt ttggtgctgt tcctatcacg gaatatctta atacagatga cggtttaggc
    63241 gattacgaaa cagaattatc tttgattgat ttgtacgact ctgcgcagtc agacacagcc
    63301 aactacatgc aagacttgtc agacgctatt ctagcgatta ttggtcgtgt gtcgtttcct
    63361 gaatacgtag atacgcaaga aaaagccatt acatatttga gagctatgcg aaaagctaga
    63421 ttgttaaatc tagaaccacc tgttgatagc gaaggacgtg aaggttctgt tgacgctaag
    63481 tatttgtata aaaaatatga cgtacaggga actgaagcat ataaaaacag aatcgctgag
    63541 gatatccaca gatttactaa tacgccagat atgacagaca ataaatttgc tggtaatcag
    63601 tccggagaag cgctcaagtg gaaagttttt ggactggatc aagaacgtgt ggatttacaa
    63661 gcgttatttg agcagtcgtt gaaacgtaga tataagctta ttgctcatgt tagtgagttt
    63721 ttaagtgaaa ttaaagattt tgatatcagc aagctaaaaa taatctttac tccaaactta
    63781 cctaaatcag accaagaaaa aataaatgat tttaaagcgc ttggtggaga actatccaac
    63841 aaaactaaga tgtttgtcac tggtattgtt gatgatccag acgaagaaga agataaaatc
    63901 atgcaagaat cgcagtcggg cagtttgcta gctcaaaaac tagaagctca aacacgtatg
    63961 tcagacaagg agttagctca tggacatgca cactaaggaa ggcaaaagtt attggcgcaa
    64021 acgtgtaaaa aaagagatgg aagccaaaga caaaaaagat gtcagtttag gaaaatctat
    64081 gaaacaaata cacgactatc acttccgtga gatagaaaaa gaaatagagt ctttttatca
    64141 acgttatgca gataaagaag cgatagactt aaagcttgct aaaaaggctg tttctgacgt
    64201 tgatataaac gcttatcaga aaaaggcaaa agaattagtt gcaagggcta acgaaatgcg
    64261 caaagaggga attaaagtca ctaaagctaa ttttacgcat caagaaaata tagacatggc
    64321 tgtttataac ttaaagatga aagttaacgc attagagctt ttgcaattaa atattgattt
    64381 agcgatgcaa aacttatcgg aagatgaata caaagcgaca aaaagattcc ttgaagatgg
    64441 ttttgaagaa gagttgaaat ttcagtcggg cttacttgga acatctgttt ctagtcaaaa
    64501 tgacattaaa aatctagcta aagcaacaat aaacgcaaat ttcaaaggcg caacgtggag
    64561 tgaaaacatt tggcaaagac aagatgacct tagaaaaata gtaaaagagg aagtttataa
    64621 agctgtaaca aaaggcgata acgctattaa gttgtctaat aagctaaaaa aagagtttga
    64681 ggtatcagac agctacgcta gacgtctagc gattacagaa catgcaaggg tacaaatgga
    64741 agttagcaaa atatcaatag aagataacgg atttaacggc tttgagatat tacccgagcc
    64801 ctccgcctgt tctatttgta aaggaatagc aagtgacggt ccgtatccta tggaaaaatg
    64861 ggatacaggc aatacagcgc caccatttca cccgcattgt cgttgtgcag tcgttggtga
    64921 agatatcgaa cataagaaag gtaaaaaatg aacaaacgca ttaagaaaaa acgcaaacta
    64981 gaatactata tcgcttcact cgtcgcagaa aacgttatgt tttcaaagga attaatcaaa
    65041 caacatgaac ggattgaaca acttgaaaaa attgtagagc ataacgcaca agcgactaac
    65101 aatgaactta gtcgcatcaa aaaacactca aagaaaaaat ggaaaaaata agtagtaaca
    65161 aaaccagttg aggctggttt ttattatgcc caaaacgtgc ttacggctat aaactgtgca
    65221 agatttaata gtcagacatg actttaaaca ggaggcgcct catggcagat ttagttaaca
    65281 atggtgtagt agacgaagta acacaagagg aagtcgaaac tcaagaagaa gttaaagcgg
    65341 aagctacatc agagaagact tttacacaag cggaagtaac tgaaatgata caacagaatg
    65401 ttaatcgtgc agttgcaaaa gctcacaaag acgctcagga gcaatttaaa gcagagcaag
    65461 acgaagctaa aaaattagca aagatgaaca gcgaagaaaa agctaactac gaaacacaga
    65521 aattgttaga agagttgcaa caactaaaag atgataagac acgcaacgaa ctaacagcag
    65581 ttgctcgtaa aatgttttca gaggctgaca ttaatgttga tgatgatgtt cttagtcttg
    65641 cagtaacttt agatgcagag caaacaaaag caaatgtcac taagctagct agcgcatttg
    65701 ctaaagcaat cgctgatgat cgcaaatcat tggcacgaca agccacccct tcaattggga
    65761 gtggttctat tacaactcaa tcaaattttg gtgcaagctt agcagaacga gctggaaaag
    65821 ttaacaccaa actattttag gaggaagaaa tgaataaacg tacagtaaaa acatcaaaag
    65881 agattttaca taacttgcca tacgaagcaa tttctgtaac tttagatgcg aatgcatttg
    65941 gtaaaggatt ggcatccgct ggaacagttt tagcaggtga tggggcatct gtttttaaag
    66001 accgcaccaa aaaagtaaaa ccagttcgaa atgctgaagt ttctggtgcc gatcatattg
    66061 atggcatttt gttaacggat gtagatctgt ctaaaggtga cgctacgggt gcacttgttt
    66121 atcgtggtac agtaaactct gataagttag ttgaccaaga gttagctaaa gacacatcaa
    66181 cactagctac taaattacca cacattgtat ttgtaaaagg aggaactaaa taatggcatt
    66241 aatttatgac gttgtaacat ctgctaacat caaaggattt tatgataaac aacaagcaaa
    66301 tgttgacttg actttgggag aaaaagcttt tccatctaaa caacaacttg gtcttaagtt
    66361 atcatttatc aaaggagcag ctggtaaacc agttagtatc aaagcggcag cgtttgacac
    66421 taaagttcca cttcgtgacc gcattgctgt agtcttatta gacgaagaaa tgccttactt
    66481 taaagaaggt atgcttgtaa aagaggctga ccgtcaacaa cttaacgttt tagcgcaaac
    66541 taaaaatcaa gaacttattg acacagtgtt atcaacaatc tttaacgatg aaactactct
    66601 aatcgctggt gctaaagcac gtcttgaagc tatgcgtatg gaagtgttgt caagtggtaa
    66661 aatccacatc aattcaaatg gtgttatgaa agatattgat tatggattaa ctggaactca
    66721 aacgactaag agtgaacaaa aatggtcaga aaaagacacc gctaaccctc ttgctgatat
    66781 cgagaaagct attgaaacag taacagagcg aggtcacgtt cctgaagcca tcgtcttaaa
    66841 ctcaaaaact tttggctata tcaaaaatgc aaaagcaacc gtaaaagtaa ttaaaccact
    66901 tgcaccagaa ggctcaattg ttactaaagc agaattaaaa tcttatcttt ctgaagaatt
    66961 gggattaaac atcttactta aagatggtgt gttcgttaac gacgcaggtg aaagcaagaa
    67021 gtatttccct gatggcgtag ttacacttgt acctaacgga aatcttggct atacagtatt
    67081 tgggacaact cctgaacagt ctgacctcat gggaggtcaa gcaacagatg cacaggtatc
    67141 tcttgtagag acaggtattg ctgttacaac tactaagact actgatcctg ttaacgtaca
    67201 gactaaggtt tctatgattg ctctaccatc attcgagcgc ttagatgaag tacagattgt
    67261 aacaagttcg gaagtatcat tataaaagga ggtaatcgtg gctctagtaa ttgaagcttt
    67321 tagagataaa gagactggtt ttatttataa agtcggtgaa gagtataacg gtgctagagt
    67381 tgagtttttg actgggaaag gtgttttaaa agctactgac acacagtcaa caaactttag
    67441 caatttaaaa gttgacgagc taaagcgtga acttgatgaa ctaggtgtta attacgattc
    67501 taaagctaaa aaagcggaat tgctagagct tttagaatct cacaccgatt aatttttgga
    67561 ggtgtttatg gaaaagataa acacgcaaac aatcattaat aatgtaaagc ttgatttaga
    67621 tatcaacgat acattacaag acaaactatt ggaaatgttg ctaaaaagga ttactgacca
    67681 cttttcagca gagtatggca ctaatgagat agatagtgcc ttttcatttg tccttgaaga
    67741 ttgtttgatt gctcgctata acaggcgagg tgctgaacgg gctaagtctg agtccgtgga
    67801 aggaagagct atcacttatt acgacttttt aaacgagttt gaaccgtatg atttaatgat
    67861 taaaagtaag cttaatattt caaatcaaaa atctaaaaaa ggtggacttt attttttatg
    67921 agatataacg atagagttac tcttttaatt aaagctaacg gtgaacctcg atatgatcca
    67981 actcttggga aacgtgttgg aggagaagtt aaaaaagatg tcgttccgtg taacatctcg
    68041 gagcaaggta tagaactatc taatctgtta gacgagaagt tagaccttga tagacgcatt
    68101 gtaaggctac gtcacagcgt taagaaggtg gatagggttt taatcaaaga taaagcttat
    68161 cgtgttgtaa cgagcagaaa taaagcttta tacgttgagg agattgttaa tgattaactt
    68221 aacattggaa ggggaacatg agttgctatc tgcgttaaaa aaagaagtta cttttgaaaa
    68281 caagcgcaaa gctgtcagaa aacacggaac taaaatgcac tcaaaggcta ttgataaagc
    68341 tgtatttaat gggcattatg agtggcaaaa aggcaaaggg cgtgtttttg ttaaacctac
    68401 aggagctact agaaggtcta ttaaattaga gtttagtaat caaagtacag ttgctaaagt
    68461 gaaggctggg acaaattatt ccggttactt ggaaacaggt acacgattaa tggaggagca
    68521 accgtttatg aagccagctc ttgatagtgt cattgacaac ttcattaaag atttagcgag
    68581 ggttgaatga tgaaacaacc ggatcaacaa ttgtttgatg agatttttaa aagactcact
    68641 gatataggat tgactgttta cgatttcctt ccaccgttgg gtacaaaata cccttttgtt
    68701 gtgatgggag acactcatat aatgccgaga gcgacaaaaa cccaattaat cggcaagtgc
    68761 tcaacaacga taaacgtctg gggagacggc atggacagaa aattgataag tgatatagtt
    68821 gctcgaatta tgcaggcggt cagtgaaata aaccaaatag aaaatagaag atggtccatg
    68881 attatagatg atagtgatac cgaaatctta aaagataata gtactaacga gaatttatat
    68941 cgtggcattt tgaacatgta ttttagtttt atataggagg aagcatggta acaagttcac
    69001 cagtttatgg taaagataat attttgatgt ttcgtgtttt gggggaaaag aaggctgcgg
    69061 ctaaattatc ttttcagacg gaacataagt ggaaatttag tagaaaaaca gatgctaaga
    69121 tcacaaaaga tggtgctata aattctgaca aaggactaga agtcacctta gaaatcaaag
    69181 gggttgctag tcgcgacgaa ttgaacacaa cattaaaaaa tgccgtttta gatggcaagc
    69241 agattgaggt ctgggatatt gacttaaata gcaacaacaa ttcagacaat aaatatgatg
    69301 cagagtacgc aataggtcga ttaggttcgt gggaagtccc ttcaaatgtt gaagaattat
    69361 ctgaaatttc cacagaaatg gctattgatg gaaaacctgt caaaggtaaa gctaccctca
    69421 ccaaagagca aatcaaggct attcaatatg tgtttaaaga cgtcactgaa acaaacggcg
    69481 atactgtttc tcatgtagaa gcaagtagtt aatctaacac aaggcaatta atttttaaaa
    69541 caagggtttt tacccttgtt atttttttaa ggagagaaaa tcaaaaatga aagaaattac
    69601 aatcgcagga aaaacttacc cattaaactt tgggtttgac ttcatccgtg agatggacaa
    69661 gaggcatttc gttgaaaaca acggttttaa atttggaaca ggtatccaaa cagcaacatt
    69721 acaattatct atcaaaaacc ccctaattct agaagatatt atcttgtcag caacacacac
    69781 tttgaatagc attccaagca aggaagaaat cgaaaaatgg gcaattaaac aagcggaaga
    69841 caataagctt gaagaggttt ttgaaggttt tttaacgagt ttgaagaaag cgccactatc
    69901 gaaagcacaa gtgaaacaat tactaaaaag catgaactaa aaattgagtc tgcaaacggc
    69961 aacaaagaga acgaaaaaaa ttctctagaa acgtacaacg aaataatagc taacattttc
    70021 gggttactga atgtaactga ctatgatgtc gctaggaata tgactgtcag agaatacaat
    70081 cttaggatga aagggtattt aatcaagcat ttagagaccg aaagaaatat ctatcttagc
    70141 gcttttataa aaagaaacgc agaagccacc aaccaaagtg gtgagtatat ttataaagat
    70201 tttaaagatt tttatgatta cgatgagcgg aagagaaaat tgttaggtga taaacccaaa
    70261 caattggata aaactattgt taatagagct aaaagggtaa aagagttgag agagaaagga
    70321 ggtattaatg tctaatcgat cgtacacagt acaagcgatt ttaaaagcaa cagatactaa
    70381 ttttacaagc actatgaaca aagttcaatc tgctgcacaa gctactattg ataaaataaa
    70441 atcaataaaa gatagcaaca tatctacgct tgggaaaatc ggcgaatata cgactatggt
    70501 aggtcaaggg atgcaaagtg ttgggcgtag cctttcaaaa tacgtcacac ttccaatagt
    70561 tgggttagga gtcgctgctg caaaaacatt tggagatttt gaatctcaaa tgaacagagt
    70621 aaaagcaatc tctggagcta ccggggcaga ttttgaaaag ttaagaaaac aagctattga
    70681 tcttggggca tcgtctgttt ttagtgctaa ggaagttgca caaggtcaag aaatgatggc
    70741 atcagcagga tttaacgcaa atcaaatctt agcagcttca cccggagtaa tgtctttggc
    70801 tgcagcatct ggtggagatc tagctcttgc ctcagaagcg gcagcgactg cggtaaatat
    70861 gtttagcttg aacgcaagtg aagccactca tgtagccgat gtgttcgcaa aagctgcagc
    70921 agatacaaac tcagaggttg cagacatggc ggaagcgtta aaatacgcag gaccagttgc
    70981 aggagctatg ggaatttcta tggaagaaac cgcagcagca ataggtatta tgtcaaacgc
    71041 tggtataaaa ggctctcaag ctggtacaac tcttagaaca gctatcacca gattagcaaa
    71101 accgacagac caaatgcaag ctgttattga tggtctcgga ttgtcttttt ttgatacgaa
    71161 tgggaagatg cgttctttga cagaaataac aggacaacta agagaaaaaa tgtctggatt
    71221 gacagatcaa caaaaatctg cagctttatc aattttattt ggtaaagagt ctctgtcagg
    71281 gatgcttgct ttaattaacg ctaccccagg cgagttatct aaattaaccg aagggttgaa
    71341 aaattcaaaa ggtgctgctg acaaaatggc aaacactatg aatagcggtc ttaaaggaac
    71401 aattgaacag ctcaaaggtt ctttagaaac agccggcatt acaatcggtg gtatattaaa
    71461 tccattgttg caaggagtta ttggtaaaat ccaagcagtc atagattggt tcaacaagtt
    71521 atctcctgct gggcaaaagc tggcagttat tattggaggt attggcgcag ctcttgggcc
    71581 tcttttggtc atttttggaa cagtaattat ttttattggt caagtaactg cttctttagc
    71641 aggagtttct acagcgttca cggcaatagc aggaatcttt tcaataattt caggacctgt
    71701 aattttagtt gttgcagcaa tcggagcatt tatagctgca cttgtagtcg cttggaacac
    71761 atctgagaca tttagaaata caattatttc tggttggaaa tcaatggttg atgcagtcac
    71821 cccactaatc gaaacattga aacaactaat attatcaatt ttcactttca tacaagctaa
    71881 cagcgctact tttattggcg gtttaaagtt agcttggagt gcgcttgttg aggctctaag
    71941 tggtttgttt ctggttataa caggcgtggt acaagttggc atgtctgtca tcaacacaac
    72001 tataaaagtg atattagcaa ttataaatgg tgattggaat gcagcatgga ccgctatcaa
    72061 atctggagta ggagcaattt gggaaggtat aaaaaacata ataattggag gtttgacgtt
    72121 tttagttagt ttatttgtaa gtatgctggg tgttttcgta tcagtttttt ctgctggatg
    72181 gagtgctata aaaggtatat tttctgtgac gctaaaattt ttggttaacg tagtagctgc
    72241 tggaatgagt gctattggaa acgagatatc atctaaaatt aatacagcaa aaagtgtcgc
    72301 tatactagca tttaatgcga tgaaaaacgg tatttcaaca gctattaatg ctgcgaaaga
    72361 agtcgtttct agtgtagtta accgtataaa aaacattctt aattcccttg ctaatattaa
    72421 cttaggagca gctggtagag ctataatgga tggattttta aatggattga cctcagcttt
    72481 tgaaaaagtc aaaaattttg tcggtggtat cgctggatgg attcaagagc ataaagggcc
    72541 aataagttac gatagaacat tacttattcc agcgggtaga gctataatgg gaggttttaa
    72601 taaatctctt acagatagct ttgaaccagt caagaaaaat gtttctagta tggctagtcg
    72661 tatttctgag gagtttcaaa acggattgaa taaacttaaa gatatcaaca tatcggaaat
    72721 tacggaaggt ttacaaagta gttttgacgt gactcattat gcaagttttg gcggactaaa
    72781 tgactcagca agaaaattag aagatgatag atttgtcgca ttgaaagatg cggttttagc
    72841 tattagagat tttgctaata gagacgttgt agtgactgtg aatggtaaag aattagctcg
    72901 aacagctgga gacaacttca cagaatacca aaaacaaaaa gaaataatga ataatagaat
    72961 gaaaggtttg atataattgg caaattttag ttataaaggc gttgatttat cgcctttttt
    73021 gaattgttta aacatagtga ggactatagg taataatagg tctattgcta ccagaaaaat
    73081 aaatgaatta ggagaagcca ttcaatcagt tagttttggc gctaagacta tttttgttac
    73141 tgttagtttt aaaactaaag aaataggagc aagtaaattt gtagacacga cagaaccgtc
    73201 gacttatagt tacgaaaact taaataaatt aagagaaaaa atagctggta ttttgcacag
    73261 taagacgacc tttaaactta cattaccaga tgaacctgac agatattata tggctgttcc
    73321 aaaaggtgat attgacttaa aaggaatatc tgattggtac gatgaaacgg ttattgagtt
    73381 ttacatccca gatggcgttg cgcattcaac tacttacaaa aagttttcgg actatacgca
    73441 agaaggcaac aagcttattt ttaatttgca aaatgatggt aatactaacg cttatccaat
    73501 aatcaaaatt aaacataact ctgaaaacgg ttatatcggt atcgcaaatg aaacgggcgc
    73561 ttttgcactt ggatcatcgg aggaagaaga tgggactatc gttcatcgca acgaatccct
    73621 ttttgattac tcaaaagcta ttgcacaagc tttagaaggt gcgcccaacg tcgcaaaact
    73681 caactatatg ccaccaacat ttgactcgga attaaagcgc atgcgccttg ataatatttt
    73741 aggctctggc aaaggtggag agtatgtagc tattggtgct agaggaacta caccgggtta
    73801 cggcgagcat gttgggactc gaacgtttat tatcaatcct gattcaaacg gagaatacac
    73861 tctcaatgag cacctgtggt ggcaacagat ttttattgca acagcgcaag accaaaaagg
    73921 atttttaaag ctttgtgtaa ctggcgaaaa cgatgaattt ttatatggtg ttgaaaccta
    73981 caagcgtaaa aacggttttg aaacagagta taactttttt gctcttgatg atgatggagt
    74041 tggctggcgc ttttataagc agtttacatt tctagcagat aggaattatc acaatccttt
    74101 ttcaatgaat agaagtagag cggttgagat ttttagagaa gaagataagt ttcgtattta
    74161 ctttaacggt gcgcatcatc atgtaactgt tccgtccctt aaagggaaaa aatcccgcaa
    74221 gatacatctt gcgatgggga catgtagcga tagctctaaa tatatcaact acaacctgtt
    74281 tgaaaaagtc aactttgaaa aaatgggtgt atctcactat aacaacattg ttaacaagta
    74341 tcagcccgga gatgaagtag ttatcaattt tgaaaacgac acagtcaaaa caaaagactt
    74401 agattctatt caagacatgg ttttaggttc gcaacttata tctatcccac ccggaaaaac
    74461 agaattagta atgcaggtat ctaagttttc tcaatctgcg ccaaacgttg agatattgat
    74521 ggaagagagg tggttataat aacgctagta atacatgatt ctaagttaca tccagttttg
    74581 cttttagaca atgataaaca aggagcactt aattattatg atgatttgtg gactagacag
    74641 ctcacaactg gttcgtcagc ctttgagttt tcagtttata aaaaaacgct gttgggtgac
    74701 aatccactta atcacaaata tcacgcacta aacgatcaag catttgtctc ttttgtacac
    74761 aaaggtaaag tacaattgtt taacatcatg caagtcgagg aaacagagac aaaaatacat
    74821 tgctattgcg aaaatcttaa tttagagtta ctaaacgagt attgcaacgc atataaagca
    74881 actaaagcaa tgtcatttga agagtatctt gtgcagtttg atattttaaa ttggggtgct
    74941 ttgacaattg gcacaaacga agttaaggac aaaaaactga cattggaatg gactggtcaa
    75001 gacactaagt tagctcgctt gttatcgatt gctaataatt ttgatgcgga agttgaattt
    75061 gaaactcaat tacacaacaa tcacacgttt aaagcgttta ttgtaaatgt gtacaaggaa
    75121 tacgaagagg gcgtgtccta tggcgtaggt cgtgaccgca gcgacatagt gttgagatat
    75181 caaaaaaatg taactggtat cactaaaaaa ttagacaagc gccagattta caacgccata
    75241 cgcccgtatg gcaaaaagac agtcaaaggc gagcgcgtta tttctaatcc tgtaactcgc
    75301 aaagtcacta aaacagttgg gtcaaatcgt acatatttag gcggagacct taaatattat
    75361 ggtcatacaa tcaaaaaagc taacgtacaa gctattatta actacgcagt gcaatataat
    75421 attttgccaa gtggaatcat cacgcaactt tatttagaga gtttttgggg tgattcgaca
    75481 gttggtaaac gtgacaacaa ctgggcaggt atgagcggtg gagcacagac gcgtccaagt
    75541 ggagtaaaag tcactactgg gatggctcgt cctgcaaacg agggtggaac atacatgcac
    75601 tatgcaagtg tagatgactt tttaaaagat tacacttatc ttttagcaaa acaagggatt
    75661 tataatgtcg tcggcaaaaa gaatatagca gactatacaa aagggctttt tagagctggt
    75721 ggagctaaat atgactatgc agcagcagga tatcaaagct acacaaattt gatgactaat
    75781 atccgaaatg gtatcaataa agtaactgga aatatcctca atacgattga taagctgtgg
    75841 caaacaccag tacagcctat aacagccgta aacgtagcta gaagagctac taagacaatg
    75901 caagcactaa atgaagctac tagacttaaa ggtcgcagaa tcggctcagg acagtgttat
    75961 gctttgtctg gttggtacgc taagaagtta gacggcgctt ggattgacag ctcggttggt
    76021 ggtattagag gtcgtatcgg aggcggtatg gctgctgcct taatcggcac tgattataac
    76081 tggggtgctt atggttggaa gctagacagg tcgcctaatg ctggcaactt gcaagctggc
    76141 ggtatctata atgttaaagc aaattttggt gctccatttt atacaacaca atgggggcac
    76201 acagggatta tcaagagtgt gtctaaaaca agagtcactg tcttagagca gaattacgct
    76261 ggacgcatgt atgtcatgga aaactcgtat gagattaacg cttttgctag aggattgcag
    76321 acagtatgtt accctcgtga aatagcgcaa ggtatgtctg tcaatggtgc aactactcag
    76381 caagttactg gtggaacaca gatatcgtac gaagaagttg tacaagaggc gcaaacagaa
    76441 acatatgaag aagaacaaat catctatatt gacaactcta tctacaaaga gtggaaagat
    76501 gaaaacggta aagtagagta ctatctcaaa aatggatttt tgtacgcacc actttcaaga
    76561 gaccgctatc catctgtttt aaccggtaat gagacacgag acaactggat acgaaaagac
    76621 atggaagtcg agactgatag tcaagaagtc ttgatgtcaa caggtctaaa agacttaaaa
    76681 gcacacgcat atccagcaat tacatacgaa gttgatggct atgttgactt agaacttggt
    76741 gatgttgtgc ggatacagga cgacggatac gagccaccgc taattctcac agcgagggtt
    76801 attgagcaag aaatatcaat aacaaatccc agctctaaca aaactaaatt cagcaatttt
    76861 gtcgaaaaag aaagtcagtt agcttccgac ttaattagtg atatgttgcg tctatacgat
    76921 gagtcaattc catacgatat acaactagcg acttcaaacg gagttgcttt taaaaatggg
    76981 gttggtgagt ctgtattaac gcctaacctg caaaaaaatg ggaaagatta cgatgctatt
    77041 tatttttata aaaatggcga ctcactgatt gagataggtc cttcgctaac agttaaagca
    77101 agtgacttta accatgtttt aaacataaca gtcgaagctt atgttaacga ggaacttgta
    77161 gcaagtacgc aaatatcctt tacagatact gaggatggag aaaaaggcga tgatggtgct
    77221 acatcatgga cagcgtgggc caattcgaaa gatggaaaag ttgactttag tattactgaa
    77281 gctaaaaata gaagatttat cggtacttat actggattaa cgcaatcaac aaattatctt
    77341 gactacaagt ggattgatat gtctgctaat gttgtcattg gtactcaaaa tttacttgat
    77401 ggtacaaaat cattttctgg aagttggttt accgaaggta caatatttga gactacaaaa
    77461 atcagcgaat atccatttga atttaagaaa tggaagtctg gaaataaggt tagtcacact
    77521 atcgagtttg atgttaaagc tggtgtaaca tacactttta cagctgctat agcaagagaa
    77581 aatgctggaa gattgtactt ctatttgtat gacttgtttg caaaccatat cacaagtaac
    77641 acacctcgtg agacgataat tgaaaatgtc actacagata tccagatgtt taaagttaca
    77701 tttgtaccgc tcagagacgg taaaataaaa ccacgctttg ccatgcttgc cagtgatgca
    77761 ggttggttta tgactggtgg atatatgctt gtcaaaggta ataaatctgg agattggcaa
    77821 gagtcagaag ctgataaagc aagtaatctt gattcaaaag ctgatcaaga attaacccga
    77881 gcacaaattc tagctcttga agaaagaact gctatagcaa gagaaaatgc aattgctgag
    77941 gctatgcagc atacactcag cgaagttgaa actaagtgga agctttggta tgacttaaat
    78001 acgatagacg aaaagcaaaa agttgcaaac gacattgctc aattgtttga tcgtacaact
    78061 gagtttaaac aacttttagg tgaggcaagt gcaaaattta gctttatcaa caacgagaca
    78121 ttgattggtg aagaggggat tgccattggt gacaaagatg gcaaagctaa gttattccta
    78181 tcaaatgaca gcatctcctt tgttactaat ggtgttgctc aaatgacact aacaggtgat
    78241 accttaacga taaagaatgg tctgtttaca gagcgtatac agattggaaa ttttgttgaa
    78301 gaagtctatg acagaaatcc attatttaat gtaatcagag cgattaggaa tagttaggag
    78361 gtgagaaatg ggaacatcaa catttagtgg aacttggggt aacaatctaa cattagatgt
    78421 acgaacaagc tatacacaaa atttagttgg taattacagt acggtcacag ttaaagtcta
    78481 cgaaaaaatt agctcctatg gttatattga ctaccctggc gaacgtacaa tgacaatagt
    78541 tgttgatggc aaatcttata atgagagtgt caacgtagac attaattatg gtcagacaaa
    78601 agaattaggt acatatagct accgtataaa tcataactct tatggatcaa aaccaaaatt
    78661 taatgttacg gtaacgctgc ctatcaattt tagcaactat gggtcggcat cagtaacaaa
    78721 ttcagttagc ttaccaacaa taaagcgtgc tagtacagcg acaatagcaa gcggaaatat
    78781 tggtagtgca gttgctatta ctatcaaaag acaatcagat acttttagac acacattgaa
    78841 atttgatttt aaaggtctaa caggcacgat tgcatcttta gttgacacat cttactcatg
    78901 gacgttgcct gcatcactgt ctgtcttgat accaaatgac cgaagtggta caggaacttt
    78961 gattgtagaa acatttacaa gtgatagtca aaaaatcggt gagaacaagt atacctttac
    79021 tgcaacagtg ccagacattg ctgcatataa gccaatattg gcaagtatta gtttatctga
    79081 tgctaacact ctgacaggta gtttgataac tggcaataac tatgtcagga acatgagtaa
    79141 attaaaggtg tcttttggta actcggcagg tgctaatggt tcaactatta gctcatataa
    79201 tgctgaaatt gttgaaaaag gcaaagctat ttttggaaat ggtagtgttt ttgatatgtt
    79261 ggattttgtt ggaacagcaa caatcagagc tacggtaact gatagcagag gtctaacatc
    79321 agaaccagtt gacacaaaaa ttaatgttat tgattatttt ttaccaattg ttacaagtgc
    79381 aaaagtagtc aggtctcagc aaaatcctga tattttacaa gtcttgccat ttgttaagat
    79441 tgcaccaatt atagttggtg gaatacaaaa aaaccaactc aaaatgtcgg tatctgttgc
    79501 accatacaat actggtatct atgcagttga tagtggcgca gctacaaata cctggtcaac
    79561 aatttcccaa atgtcaggtg cccctttaaa tcttggcggc acttatgaca agtcaaaatc
    79621 atggcttgtt aagatttcgg tgagtgataa cctaatgtca gcaacaccaa taatccaacc
    79681 agttgcaagt gagtttgtac ttgtaactaa agcaccttct ggtgttgcat ttgggaaaat
    79741 ttgggaacat ggtattattg atgccaaagg cgatgtttat gttgatggta atatttattg
    79801 tggcgataag ccaatccaac aaaaacaact cgctcttaac aacggcggtt cttttagaca
    79861 tgacgacact gacctaaata gcttgcaaga cacaggtttt tattgtgtat ttagaggtgc
    79921 taatagacca agtggtgctg gaccgggcta tttaacagtt gtaagacacg agacagccaa
    79981 ttacgcttat caacaatttt atgatcgaac aaataaaact atatttacca gagtattgga
    80041 aaatggggtt tggagcggtt ggagtgagta tgttaaaaaa gatagctcac aaacgactgg
    80101 ctggataaca attggcaatg gttttagtta taggcagaca agcagtacag tcaccgttaa
    80161 gtacaacttt gcgacgaatg gcatagataa gttgacggtt ggctctatgc ctacgaattt
    80221 gatacctagc gacatgatgt ttgcagtaac ggcttggact atacaactca atgttttaaa
    80281 tgttcaagtg agcgcagacg gtcgtattct gtggtttaat ccatcaaaat ggactgttaa
    80341 cgtaaaaggt caaattcagt ggacgattta aaggaggaaa actattggaa attttaaaca
    80401 aatatcctgt aatgctagaa gataaaagca ttgcaaaagt taatgcaatt gtggcagttg
    80461 atttacctca cgtaagaggt aacttaactt ttgacttacc agttgacttt gataataaat
    80521 cttttgcaga aacgcttgaa aagtgtgagc agatatttta cgacgaaaag tataaagata
    80581 aagctcagtc tgaaaaaatg actgaactaa gtacatcaac atcaacaggc acacaaacac
    80641 ttatcaatct gataagtacg ctttacgcaa aagaggtttt aaaagatgaa gatcttattg
    80701 ctattggtta gaattttttt acaggaagaa gggatagata tgatgattaa attatttgcg
    80761 attgacttat attatggacg tatggcttgg tcaagttttg ttaaaaaggg attttcagag
    80821 tttattaata acaaaacaaa agagcaactt gcaattatgt gcgatgaaga attacttgct
    80881 gaaattttag caagttagtg aggtagtcgg atgacagtag aacaagcaga aagaatcgct
    80941 caatcacaat ttgtgtgggc tattctcttt atcttgcttt ttatgattgt ggttggctat
    81001 ctggtgcgaa cgtctgataa gcgtgagaaa aagctaatgg atttccatga ccaatcaaaa
    81061 tcagaatcta acaaacgtga agagtggctc aaaggtcact tagataaaaa tacagaacag
    81121 ttacaggaca tttctcagac cattggtgtt gtccaaaagg agatgtctta tatgagtgac
    81181 cgcattggtc gtctagaaaa agaggagaaa taagatgaac gaatttacaa cacaaatcat
    81241 tacagcatca gtaccaattt ttggtatcat cgcaggtatt ttaacgcatg aagttaaaaa
    81301 actacttatc aaaaaaggag gcgaaaaggc tgttaaaatc gcagaaattg tcgctcgtaa
    81361 tgctgttgag gcagttgaac aaatatctgt ggaagttggt attaagggac aagataagct
    81421 aacagaagct aaaaccgctg ttattgatgg tttatcgcaa tataatatta atcttacaca
    81481 gacacaactt gaaactttta tcgaagctgc ggttaaacgc atgaatgatg agtggaagaa
    81541 aggtcaataa tatggtaatt aatattgagc aagctatcgc atggatggca tctagaaaag
    81601 gtaaagttac ttactcgatg gactatcgaa atggtccttc atcttacgat tgctctagct
    81661 ctgtctattt tgctctacgc tcagcaggcg catcagataa tggctgggca gtaaatacag
    81721 aatatgagca cgattggttg ataaaaaacg gttatgttct tattgcagaa aatactaatt
    81781 ggaatgcgca aagaggtgat atttttattt ggggtaaacg tggggcttcg gctggagctt
    81841 ttggacatac tggtatgttt gttgatccag acaatattat tcactgtaat tatggttata
    81901 actcaattac agtgaataac catgatgaaa tctggggata taatggtcaa ccgtatgttt
    81961 acgcttatcg ttactctggg aaacagtcaa atgctaaggt tgataataaa tcagttgtat
    82021 ctaaatttga aaaagagtta gacgttaaca caccgctaag taattcaaat atgccatatt
    82081 acgaagcaac aatttcagaa gattattacg ttgagtccaa gcctgacgtc aatagcactg
    82141 ataaggagtt gcttgttgct ggtactagag ttcgagttta cgaaaaagta aaaggctggg
    82201 caaggatagg cgctccacag tctaatcagt gggtagaaga tgcttactta attgatgcaa
    82261 cagatatgta aaccaacaga gcgatataaa tgtcggtctg ttaatggtgt aagttacacc
    82321 gcaactaaaa aaacaattta ggaggtaaaa ttcctttaga taagacaaat gccctcgctt
    82381 ttgcgggggc tatttttatt gaaatattga aatctcttta taaaaatagt aaaatagttt
    82441 cgctattata aagaaagttg ttatcaatga ataatctagt tcttcctca
//
LOCUS       DATRNK010000002        75455 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.002, whole genome shotgun sequence.
ACCESSION   DATRNK010000002 DATRNK010000000
VERSION     DATRNK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 75455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75455
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.002"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(131..685)
                     /gene="raiA"
                     /locus_tag="VBC52_000110"
     CDS             complement(131..685)
                     /gene="raiA"
                     /locus_tag="VBC52_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEO6881721.1"
                     /translation="MIKYSIRGENIEVTEAIREYVETKLSKVEKYFNEAQELDTRVNL
                     KVYREKTAKVEVTILIDSITLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKYRE
                     KIPASQVFTTEFEAEPDEEAVSQRIVRTKNVNLKPMDVEEALLQMELLGHDFFIYTDA
                     EDNTTNVLYKREDGELGLIEAKGN"
     gene            complement(762..1427)
                     /locus_tag="VBC52_000111"
     CDS             complement(762..1427)
                     /locus_tag="VBC52_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEO6881722.1"
                     /translation="MTCLLCHEIDLSQLTFVELMLLKPKQNVICQTCKGSFEALSREM
                     GCQTCCKQIPQKQCQDCIYWGKKGIEVNHFSLYRYNEAMKKYFSLFKFQGDYLLKDVF
                     TKEIKAALKKYKGYTIVPVPLSHEGYQNRQFNQVIAFLQSANIPYKNILSKKDGGKQS
                     ANNKEERLKQVQQFTLKNEAELGDNLLIVDDIYTTGATIAQIRKLLEEKGIKNIKSFS
                     LAR"
     gene            complement(1427..2716)
                     /locus_tag="VBC52_000112"
     CDS             complement(1427..2716)
                     /locus_tag="VBC52_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6881723.1"
                     /translation="MENYLGRLWTKAQLSEQLRKIAISLPSFIKKGSDYICTRCSSSV
                     AKNCQLPTGNYYCRECIVFGRVTSNENLYYFPQKTFSKTNSLKWKGELTPYQNEVSEE
                     LLKGISSKENLLVHAVTGAGKTEMIYHSVAKVIDTGGSVCIASPRIDVCLELYKRLSN
                     DFRCAITLMHGESPSYQRSPLTIATTHQLLKFYHAFDLLIVDEVDAFPYVDNPILYQG
                     VKQALKENGTSIFLTATSTTELERKVARKELKKLHLARRFHANPLVIPEMVWVSGIQK
                     SLQTQKLPPKLYQLINKQRQTRYPLLLFFPHISEGQVFTEILRQAFPMEKIGFVSSKS
                     TSRLKLVQDFRDNKLSILVSTTILERGVTFPSVDVFVIQANHHLFTKSSLVQISGRVG
                     RALERPEGLLYFLHDGKSKSMHQAIKEIKNMNHIGGF"
     gene            2772..3416
                     /locus_tag="VBC52_000113"
     CDS             2772..3416
                     /locus_tag="VBC52_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEO6881724.1"
                     /translation="MNYKTIKSDGIVEEEIKKSRFICHLKRVESEEEGRNYITQIKKA
                     HYKANHSCSAMVIGEKGDIKRSSDDGEPSGTAGIPMLTVLEKQGLTNVVAVVTRYFGG
                     IKLGAGGLIRAYSGSVANTIKEIGVVEVKEQIGIRIQLTYPQYQTFDNFLKEHHLQEF
                     ETEFLEAVTCKIYVDPKEFEHTITNLTEFYQGKALLTEEGSQIVEIPFKAETDK"
     gene            3507..4432
                     /gene="cysK"
                     /locus_tag="VBC52_000114"
                     /pseudo
     CDS             3507..4432
                     /gene="cysK"
                     /locus_tag="VBC52_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646141.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
     gene            complement(4584..5672)
                     /locus_tag="VBC52_000115"
     CDS             complement(4584..5672)
                     /locus_tag="VBC52_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEO6881725.1"
                     /translation="MKVFASPSRYIQGKDALFQSIEHIKSLGQTPLILCDDVVYNIVG
                     ERFLSYLQDDLLPHRVSFNGEASDNEINRVVAVAKEKNSDLIIGLGGGKTIDSAKAIA
                     DKVNLPVVIAPTVASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVIAQAPK
                     RLLASGIADGLATWVEARAVLQKNGIAMAGGRQTLAGVAIAQACERTLFNDSLQALAA
                     CDAKVVTKALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALSGDIHHLTHGEKVAY
                     GTLTQLFLENRPKEEIDRYINLYQAIGMPTTLAELHLGDATYEELLKVGQQATIEGET
                     IHEMPFKISAEDVAAALLTVDRYVSNHQ"
     gene            complement(5740..6408)
                     /locus_tag="VBC52_000116"
     CDS             complement(5740..6408)
                     /locus_tag="VBC52_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO6881726.1"
                     /translation="MEFLLDTLNLEAIKKWHHILPLAGVTSNPTIAKKEGDIHFFQRI
                     RDVREIIGREASLHVQVVAKDYQGILDDAAKIRQETDDDIYIKVPVTPDGLAAIKTLK
                     AEGYNITATAIYTSMQGLLAISAGADYLAPYFNRMENLDIDATQVIKELAQAIERTGS
                     SSKILAASFKNASQVTKALSQGAQSITAGPDIFESVFAMPSIAKAVNDFADDWKASQH
                     SEHI"
     gene            complement(6418..8874)
                     /locus_tag="VBC52_000117"
     CDS             complement(6418..8874)
                     /locus_tag="VBC52_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEO6881727.1"
                     /translation="MTKQLLTQKYTNDVQQNSQKHFGYLTERMYSYRDKVLDKKPFID
                     AERAILVTEAYQKHQEKPNVLKRAYMLQNILEKMTIYIDDETMIVGNQASSDKDAPIF
                     PEYTLEFVVNELDLFEKRDGDVFYITEETKEQIRNIAPFWENNNLRARAGVMLPEEVQ
                     VYMETGFFGMEGKMNSGDAHLAVNYQKLLEEGLIGFEKKARKAKADLDLTKPESIDKY
                     HFYDSILITIEAVKTYAERFAILAKKQAKTANAKRRQELLDIASICERVPYYPAETFA
                     EAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKSDLEAGRETEDSIVERLTNLWI
                     KTITINKVRSQAHTFSSAGSPLYQNVTIGGQTRHKEDAVNPLSFLVLKSVAQTHLPQP
                     NLTVRYHANLDKSFMNEAIEVMKLGFGMPAFNNDEIIIPSFIKKGVSEEDAYDYSAIG
                     CVETAVPGKWGYRCTGMSYINFPKVLLITMNDGIDPASGKRFAPSYGHFTQMTSYKEL
                     KEAWDKTLRYLTRMSVIVENAIDISLEREVPDILCSALTDDCIGRGKHLKEGGAVYDY
                     ISGLQVGIANLSDSLAALKKLVFEEKRLTTLEVWQALQSDYAGPRGEEIRQMLINEAP
                     KYGNDDDYADSLVRECYDVYVEEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGT
                     LATPDGRHAGTPLAEGCSPSHNMDKKGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTL
                     AKEEDKQKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAF
                     FNVLSKATQDDIIARTEHAL"
     gene            complement(9055..10356)
                     /locus_tag="VBC52_000118"
     CDS             complement(9055..10356)
                     /locus_tag="VBC52_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6881728.1"
                     /translation="MSKFDSQKIITPIMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     LGQLPFKGLNQAIANVFGPEWTEPFIQVYSGTFAIMGLISCFAIAYAYAKNSSVEPLP
                     AGVLSLSSFFILMKSSYIPVKGEAIADAISKVWFGGQGIIGAIIIGLVVGAIYTWFIQ
                     HHIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYLIAKVTTGGTFIEMIYDIIQVP
                     LQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKSLLAANRLTL
                     DNGAHIVTQQFLDSFLILSGSGITFGLVIAMLFAAKSKQYKALGKVAAFPAIFNVNEP
                     IVFGFPIVMNPVMFLPFILVPVLAALIVYGAIAVGFMQPFSGVTLPWSTPAIISGFMV
                     GGWQGALVQIVILAISTAVYFPFFKIQDNITYKNECEMERG"
     gene            complement(10358..10678)
                     /locus_tag="VBC52_000119"
     CDS             complement(10358..10678)
                     /locus_tag="VBC52_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6881729.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKIAADKEGIEAHIEAYSQGKIAD
                     YAKDLDVALLGPQVSYTLDKSKSICDEYGVPIAVIPMADYGMLDGVKVLKLALSLLET
                     RKEA"
     gene            complement(10695..11018)
                     /locus_tag="VBC52_000120"
     CDS             complement(10695..11018)
                     /locus_tag="VBC52_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEO6881730.1"
                     /translation="MEIIVADQIIMGLILNAGDAKQHIYQALKLAKEGNFAESKIEIE
                     LADSALLEAHNLQTQFLAQEAGGTRTDISALFIHSQDHLMTSITEINLIKEIIDLRQE
                     LHIQK"
     gene            complement(11222..12205)
                     /locus_tag="VBC52_000121"
     CDS             complement(11222..12205)
                     /locus_tag="VBC52_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEO6881731.1"
                     /translation="MKLERQKLLAKVAYLYYMEGKSQSEIANELGIYRTTISRMLAKA
                     REEGLVRIEISDFNPEIFQLESYFKSKYHLKDIEIVSSRKDSDTSEIEKDLAHVAAAM
                     IRKKIKENEKVGIAWGRTLSKVVEAMRPHPVSQVSFVPLAGGPSHINARYHVNTLVYE
                     MSRRFQGSCTFINATLVQENANLAKGILTSKYFEGLMDNWEKLDVAIVGVGGKPKSNE
                     QQWLDLLNQDDFQCLDEEAAVGEITCRFFNHSGDPVNQHLAKRTIGITLEQLQKVPNR
                     IAVAHGNYKAAALLAVLKKGYINHLVTDFSTALNILRLDKDTFVDTIYQKS"
     gene            complement(12324..13079)
                     /locus_tag="VBC52_000122"
     CDS             complement(12324..13079)
                     /locus_tag="VBC52_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6881732.1"
                     /translation="MNRLENIISLVSQYQKIDVNTLSELLQVSKVTIRKDLDKLEGKG
                     LLHREHGYAVLNSGDDLNVRLSFNHKTKKEIAALAANMVSDNDTILIESGSTCALLAE
                     NICQTKRNVTILTNSCFIANYLREYDSCQIVLLGGEYQSSSQVTVGPLLKKMISLFHV
                     SLAFVGTDGFDPKLGFMGRDLMRSEVAQEMANAADEVIILTDSSKFNQTALVEQLPLS
                     TVSQVITDKHPNSEIANLFQEAEITIQSLLQVP"
     gene            13200..13976
                     /locus_tag="VBC52_000123"
     CDS             13200..13976
                     /locus_tag="VBC52_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEO6881733.1"
                     /translation="MTNEKGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQ
                     KMVPETMRDAITNESVIVGEEKSVDDIIEEVLKDIDFYEESGGGITLSGGEIFAQFEF
                     AKAILKRAKSLGIHTAIETTAYTRHEQFIDLIQYVDFIYTDLKHYNSLKHQEKTMVKN
                     ASIIKNIHYAFANGKTIVLRIPVIPNFNDSLEDAEEFACLFDRLDIRQVQLLPFHQFG
                     QNKYQLLNRQYEMEEIAALHPEDLLDYQAIFSKYNIHCYF"
     gene            complement(14023..14379)
                     /locus_tag="VBC52_000124"
     CDS             complement(14023..14379)
                     /locus_tag="VBC52_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEO6881734.1"
                     /translation="MKIGDKLKGTVTGIRPYGAFVSLEDGRTGLIHISEIKTGYIDNI
                     YDVLSVGDEVYVQVIDVDEFTQKASLSLRTLEEERHHIQHRHRFSNNRLKIGFKPLEE
                     NLPSWVEEGLAYLNNK"
     gene            complement(14381..15781)
                     /locus_tag="VBC52_000125"
     CDS             complement(14381..15781)
                     /locus_tag="VBC52_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEO6881735.1"
                     /translation="MDAKTKYKAKKIKAVFFDIDDTLRVKDTGYMPPSILKVFKALKD
                     KGIVVGIASGRARYGVPKEVQDLNADYCVKLNGAYVKDKDKNIIFHRPIPAEYVEQYK
                     KWADTVGIKYGLAGRHEAVLSDRDDLVNDAIDIVYSDLEVNPDFNKEHDIYQMWTFED
                     KGDSLHLPEPLAEHLRLIRWHDHSSDVVLKGTSKALGVSKVVEHLGLKPENILVFGDE
                     LNDLELFDYAGLAVAMGVSHPEAQKKADFITKKVEEDGILYALEELGLIEKELTFPQV
                     DIENTEGPVAVIKTNHGDMTVKLFPDHAPKTVANFIGLAKQGYYDGIIFHRIIPDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNVRGALSMANAGPNTNGSQFFIVQNTKIP
                     YAKKELERGGWPTPIAELYAGQGGTPHLDRRHSVFGQLVDQSSFEVLDEIAAVETGSQ
                     DKPLEDVVILTIEVKE"
     gene            complement(15823..16464)
                     /locus_tag="VBC52_000126"
     CDS             complement(15823..16464)
                     /locus_tag="VBC52_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6881736.1"
                     /translation="MDKIKIVLVDDHEMVRLGLKSFLNLQADVEVIGEASNGLEGIKK
                     ALELRPDVVVMDLVMPEMDGVEATLALLKDWPEAAILVLTSYLDNEKIYPVIEAGAKG
                     YMLKTSSAAEILNAIRKVSRGEQAIENEVDKKIKAHDKCPALHEGLTARERDILNLLA
                     KGYDNQRIADELFISLKTVKTHVSNILGKLNVADRTQAVVYAFQHHLVPQDDE"
     gene            complement(16457..17476)
                     /locus_tag="VBC52_000127"
     CDS             complement(16457..17476)
                     /locus_tag="VBC52_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6881737.1"
                     /translation="MKKHHYFLAFFYGSVIIFAICFVIIDSLGVNLVHLYQTSRLWLI
                     EQLIFSIFFLSLAVTILLLLTWFLLDDNSKRQINHNLRRILNNQSINVTDDGTEISTN
                     IQRLSKKMNLMTASLQSKENSRILKSQEIVKQERKRIARDLHDTVSQDLFAASMVLSG
                     IAQNVSQLDVDQVGSQLLAVEEMLQHAQNDLRILLLHLRPVELENKTLSEGFRMILKE
                     LTDKSDIEVVYHESILTLPKKIEDNIFRIGQEFISNTLKHSQASRLEVYLNQTENELQ
                     LKMIDNGIGFDMDSVYDLSYGLKNIEDRVEDLAGNLQLLSQPGKGVAMDIRLPLVNQS
                     EDKNG"
     gene            complement(17473..18168)
                     /locus_tag="VBC52_000128"
     CDS             complement(17473..18168)
                     /locus_tag="VBC52_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEO6881738.1"
                     /translation="MKKFQFFLLVEAVVLVMGLMKILSDDWTSFIFILALILLALRFY
                     NNDSRHNFLLTTSLLLLFLIFMLNPYIIAAVVFAVLYVLINHFSQVKKKNRYALIQFK
                     NHQLDVKTTRNQWLGTDQHESDFYAFEDINIIRISGTDTIDLTNVIVSGQDNVIIIQK
                     VFGDTKVLVPLDVAVKADISSVYGSVQYFDFEEYDLRNESIKLSQEEEYYLLKRVKLV
                     VNTIAGKVEVSRK"
     gene            complement(18329..20284)
                     /gene="pknB"
                     /locus_tag="VBC52_000129"
     CDS             complement(18329..20284)
                     /gene="pknB"
                     /locus_tag="VBC52_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEO6881739.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLARDLILDNEEVAIKVL
                     RTNYQTDQIAVARFQREARAMAELTHPNIVAIRDIGEEDGQQFLVMEYVDGFDLKKYI
                     QDNAPLSNNEVVRIMNEVLSAMSLAHQKGIVHRDLKPQNILLTKKGTVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSILAENKSVPQALENIVIKATAKKLTDRYKTTYEMGRDLSTALSST
                     RHREPKLVFNDTESTKTLPKVTSTVSSLTTEQLLRNQKQAKTTEKITPDSASNDKTKS
                     KKKASHRLLGTIMKLFFALCVVGIIVFAYKILVSPTTIRVPDVSNKTVAQAKMTLENS
                     GLKVGAIRNIESDSVSEGLVVKTDPAAGRSRREGAKVNLYIATPNKSFTLGNYKEHNY
                     KDILKDLQGKGVKKSLIKVKRKINNDYTTGTILAQSLPEGTSFNPDGNKKLTLTVAVN
                     DPMIMPDVTGMTVGEVIETLTDLGLDADNLVFYQMQNGVYQTVVTPPSSSKIASQDPY
                     YGGEVGLRRGDKVKLYLLGSKTTNNSSSTPIDSSASSSTGTTTSDSVSSSTDASTSDS
                     SSTSTSSSTLPSDSTTNTGTANNPLTQ"
     gene            complement(20284..21021)
                     /locus_tag="VBC52_000130"
     CDS             complement(20284..21021)
                     /locus_tag="VBC52_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEO6881740.1"
                     /translation="MEISLLTDIGQRRSNNQDFINQFENKAGVPLIILADGMGGHRAG
                     NIASEMTVTDLGSDWAETDFSELSEIRDWMLVSIETENRKIYELGQSDDYKGMGTTIE
                     AVAIVGDNIIFAHVGDSRIGIVRQGEYHLLTSDHSLVNELVKAGQLTEEEAASHPQKN
                     IITQSIGQANPVEPDLGVHLLEEGDYLVVNSDGLTNMLSNADIATVLTQEKTLDDKNQ
                     DLITLANHRGGLDNITVALVYVESEAV"
     gene            complement(21059..22381)
                     /gene="rsmB"
                     /locus_tag="VBC52_000131"
     CDS             complement(21059..22381)
                     /gene="rsmB"
                     /locus_tag="VBC52_000131"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEO6881741.1"
                     /translation="MANDWKKSARGLALMTLEEVFDKGAYSNIALNKSLKKSRLSDKD
                     RALVTEIVYGTVARKITLEWYLSHFIVDRDKLELWVYHLLLLSLYQLLYLDNIPDHAI
                     VNDAVTIAKNRGNKKGAEKLINAVLRRVSSETLPEIASIKRQNKRYSVAYSMPVWLVK
                     KLIDQYGETRALAIMESLFERNKASLRVTDLSQKQTIKETLNVRDSHIAETALVADSG
                     NFSSTSFFQDGLITIQDESSQLVAPTLKVSGNDQVLDACSAPGGKTSHIASYLTTGAV
                     TALDLYDHKLELVMENAKRLGLSDKIKTKKLDASKAHEYFLEDTFDKILVDAPCSGIG
                     LIRRKPDIKYNKANQDFEALQEIQLSILSSVCQTLRKGGIITYSTCTIFEEENFQVIE
                     KFLENHPNFEQVELSHTQEDIVKRGCISISPEQYHTDGFFIGQVKRIL"
     gene            complement(22371..23306)
                     /locus_tag="VBC52_000132"
     CDS             complement(22371..23306)
                     /locus_tag="VBC52_000132"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEO6881742.1"
                     /translation="MTKLLFMGTPDFSATVLKGILADGKYDVLAVVTQPDRAVGRKKE
                     IKMTPVKEVALENNIPVYQPEKLSGSPELEQLMTLGADGIVTAAFGQFLPTKLLESVG
                     FAINVHASLLPKYRGGAPIHYAIINGEKEAGVTIMEMVAKMDAGDMVSKASVEITDED
                     NVGTMFDRLAVVGRDLLLDTLPGYLSGDIKPIPQNEEEVSFSPNISPDEERIDWNKSS
                     RDIFNHVRGMYPWPVAHTLLEGNRFKLYEVTMSEGKGSPGQVIAKTKNSLTVATGDGA
                     IELKSVQPAGKPRMDIKDFLNGVGRNLEIGDKFGE"
     gene            complement(23353..25743)
                     /locus_tag="VBC52_000133"
     CDS             complement(23353..25743)
                     /locus_tag="VBC52_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEO6881743.1"
                     /translation="MARKLAQVIVDIPLMQTDKPFSYAIPKDLEDLVQVGVRVHVPFG
                     RGNRLLQGFVVGFRDDDELETKDIAEVLDFEPVLNQEQLDLADQMRHTVFSYKISILK
                     SMLPSLLNSQYDKLLLATDTLPSEDREDLFGHKTEIVFSSLSSQDAKKAGRLIQKGFI
                     EVQYLAKDKKTIKTEKIYKINRTLLEKSQIAARAKKRLELKEFLLENPQPGRLTALNK
                     QFSSPVVNFFREEGIIEVIEKEASRSDNYFKGILKTDFLDLNQEQAKVVKIVVDQIGK
                     EQNKPFLLEGITGSGKTEVYLHIIDNVLKLGKTAIVLVPEISLTPQMTNRFISRFGKQ
                     VAIMHSGLSEGEKFDEWRKIKSGQAKVVVGARSAIFAPLENIGAIIIDEEHESTYKQE
                     SNPRYHARDVALLRAEYYKAVLLMGSATPSIESRARASRDVYKFLELKHRANPKARIP
                     QVEIIDFRNFIGQQEVSNFTSYLLDKIRDRLDKKEQVVLMLNRRGYSSFIMCRDCGYV
                     DQCPNCDISLTLHMATKTMNCHYCGFEKPIPRTCPNCNSKSISYYGTGTQKAYEELLK
                     VIPDAKILRMDVDTTRQKGGHESILKRFGNHEADILLGTQMIAKGLDFPNVTLVGVLN
                     ADTSLNLPDFRSSERTFQLLTQVAGRAGRAEKEGEVVIQTYNPNHYAIQLAQKQDFEA
                     FYQYEMNIRRQLGYPPYYFTVGLTLSHKDEEWLIRKSYEVLSLLKQGFSDKVKLLGPT
                     PKPIARTHNLYHYQIIIKYRFEDNLELVLNRLLDMTQDKENRDLRLAIDHEPQNMM"
     gene            complement(25817..26131)
                     /locus_tag="VBC52_000134"
     CDS             complement(25817..26131)
                     /locus_tag="VBC52_000134"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEO6881744.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGEKATQDFKSV
                     KSTLRALEEIESGNVVIHPDPSAKRASVRARIEAERLAKEEEERKIKEQIAKEKEDGE
                     KI"
     gene            complement(26154..26783)
                     /gene="gmk"
                     /locus_tag="VBC52_000135"
     CDS             complement(26154..26783)
                     /gene="gmk"
                     /locus_tag="VBC52_000135"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEO6881745.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFDYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEALIKEGQMLEYAEYVGNYYGTPLSYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFIFLTPPDLEELEERLVGRGTDSPEVIAQRIERAKEEIALM
                     REYDYAVVNDQVSLAAERVKRVIEAEHYRVDRVIGRYTNMVKETDKKLS"
     gene            complement(26950..27654)
                     /locus_tag="VBC52_000136"
     CDS             complement(26950..27654)
                     /locus_tag="VBC52_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEO6881746.1"
                     /translation="MQKFKERFDTLSDAILAIAMTILVLEIKTPATMGDIGDFTRNIG
                     LFIVSFVVVFNFWYERAQNSLDAQKTNDEIIALDIIEHLGICLIPLFTKFMISFENHN
                     FAVMAYGLLTLLVGLTSDIIRIRLASYDLVTIPSELKERVIKVMTTFAIRSVVVRFII
                     IILAYFLPEVGIFAYLVIPLFMFWRRSRAGKYYAKKGIEAPSYIKLFIQIGERAPEDF
                     TNSSSGIGGVKRLYKK"
     gene            complement(27704..28294)
                     /locus_tag="VBC52_000137"
     CDS             complement(27704..28294)
                     /locus_tag="VBC52_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6881747.1"
                     /translation="MKLLVAEDQSMLRDAMCQLLLMEESVSTIDQAGNGGEAIAILSN
                     KAIDVAILDVEMPILSGLDVLEWVRKYQNVKVIIVTTFKRSGYFQRAIRSNVDAYVLK
                     DRSVADLMKTIQKVLSGGKEYSPELMENVISNPLSEQEIKILSLIAQGKTSKEIACTL
                     FLSNGTVRNYTSNIFDKLGVNNRLEALKIAREKDWL"
     gene            complement(28296..29402)
                     /locus_tag="VBC52_000138"
     CDS             complement(28296..29402)
                     /locus_tag="VBC52_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6881748.1"
                     /translation="MKNKKNNYDLMYFIPLVFLIYPIGGILYYHYPFWTLFFTLAFVG
                     AYLYSVIIRGESKYHMIAWSTMLTYIFYMTIFINSGFIWYIYFLSNLLVYRFRDKLKS
                     FRFISFACTLATVVFLCFFKASDFGDRIMFLIVPIFCIGYMWIAIENRNSEEQREKIA
                     EQNQYINILSAENERNRIGRDLHGIGRDLHDSLGHTFAMMTLKTELALKLLEKRNYDK
                     VQKELSELNHISHQSMSEVRQIVSNLKYRTVVEEIDELYRLFQLSNIKLTVVNKLETS
                     QLSPVTQSTITMILKELSNNIVKHAEADSVELSLVRQGATINIEMIDNGCGFTNLDGD
                     ELHSIQERLTIVEGTLTILSRSKPTHIQVVLKEV"
     gene            complement(29399..30139)
                     /locus_tag="VBC52_000139"
     CDS             complement(29399..30139)
                     /locus_tag="VBC52_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6881749.1"
                     /translation="MRIFKALLKMEFLLTKRQLANLIMAIGMPVAFFLFFSGFMGEGL
                     TKAIEAIYVRNYMITMAGFSSLSFAFFTFPFSMKDDQLSNRMQLLRHSPVPMWQYYLA
                     KIIRILFYYCLAITVVFLTGHILRQVSMPIEQWMQSFLLLLGGATCFIPFGLLVSYFK
                     NTELMSMVANICYMSLAVLGGMWMPITMFPKWLQALSKLTPTYHLTQVILSPFANSFA
                     GFSLIILIGYGIIMLVIAYLLSQKRHSI"
     gene            complement(30126..31022)
                     /locus_tag="VBC52_000140"
     CDS             complement(30126..31022)
                     /locus_tag="VBC52_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6881750.1"
                     /translation="MAKDIIKVDHIFKSIGQKTILEDISFSIASNQCVALIGPNGAGK
                     TTLMSTLLGDISISSGSLTIFNLPAHHNRLKYKVAILPQENVLPSKFTVRELIDFQRC
                     LFPEVLPMSLILDYLQWSDTHLQQFTETLSGGQKRLLAFVLTLVGKPQLLFLDEPTSG
                     MDTSTRQRFWELIATLKKEGVTIVYSSHYIEEVEHTADRILVLHKGKLLRDTTPFAMK
                     QEKTEKLFTVPLSYQKLLPTYLITECEAKSDSITFVTGEAEAVWKILADNGCPIEAIE
                     MTNRTLLNRIFETTKEVKHENL"
     gene            complement(31151..32758)
                     /locus_tag="VBC52_000141"
     CDS             complement(31151..32758)
                     /locus_tag="VBC52_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEO6881751.1"
                     /translation="MFNIILAMVCALIGLIIGYVAISMKMKSSKEAAELTLLNAEQDA
                     VDLRGKAEIEAEHIRKAAERESKAHQKELLLEAKEEARKYREEIEKEFKSDRQELKQM
                     EARLTDRASSLDRKDENLSNKEKMLDSKEQSLTDKSRHINEREQEIATLETKKVEELS
                     RIAELSQEEAKDIILADTEKDLAHDIATRIKEAEREVKDRSNKIAKDLLAQAMQRLAG
                     EYVTEQTITTVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVVLSGFDPI
                     RREIARMTLESLIQDGRIHPARIEELVEKNRLEMDQRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSYGQNVLRHSVEVGKLAGILAGELGENVDLARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPIVVNTIASHHGDVEPDSVIAVIVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIANGFEGVQNAFALQAGREIRIMVQPGKVSDDQVVIMSHKVRE
                     KIEQNLDYPGNIKVTVIREMRAVDFAK"
     gene            32947..33429
                     /locus_tag="VBC52_000142"
     CDS             32947..33429
                     /locus_tag="VBC52_000142"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEO6881752.1"
                     /translation="MTKEVVVESFELDHTIVKAPYVRLISEEVGPVGDIITNFDIRLI
                     QPNENAIDTAGLHTIEHLLAKLIRQRINGLIDCSPFGCRTGFHMIMWGKQDATEIAKV
                     IKSSLEAIAGGVTWEDVPGTTIESCGNYKDHSLHSAQEWAKLILSQGISDNAFERHIV
                     "
     gene            complement(33654..35117)
                     /locus_tag="VBC52_000143"
     CDS             complement(33654..35117)
                     /locus_tag="VBC52_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881753.1"
                     /translation="MSEDQKHPFFEPKKETDGLEFKDAKEMTVEEAVRKDSEIKAGIT
                     EEDSILDKYIKQHRDEVASQKFETKSSDFANLDTASLDDFIKKQREELSAMLAAEELS
                     KKLDNSVSQEQDTEANAVSPKEESSQEQENSVTPVPPLNTEAEPTATEPDSTIADSEE
                     YKSSSKKRGGIVGTLIALILLLIVAIFGYNYFKNNNSTNSQTATSQSSSSKATTTSSE
                     EDKKASQNLDNFNKSYANFFVDDKKTQLKNSEFDKLSELEKKVDALKGTKYYGKVKVK
                     FDSLKRQIDAVKAVNDKFKSPAVVDGKKSEKLEVKDGANFDSLDSKTLNTGNASLDSL
                     LHSIVSTGRNQVKQSEEQASSNKVSDTQITEQPNVTNGQSSSSAATINNQAAGTASGN
                     LERNRSRVPYNNAAIADTGNPAWIFNPGVLEKIVATSQARGYFSGNNYILEPVNIING
                     NGYYNMFKLDGTYLFSINAKTGYFVGNAPGRADSLDY"
     gene            complement(35130..36284)
                     /locus_tag="VBC52_000144"
     CDS             complement(35130..36284)
                     /locus_tag="VBC52_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEO6881754.1"
                     /translation="MKESFKLIATAAAGLEAIVGREIRNLGIDCQVENGRVRFHGDIK
                     TIIETNLWLRAADRIKIIVGEFPARTFEELFQGVYGLDWENYLPLGAKFPIAKAKCVK
                     SKLHNEPSVQAISKKAVAKKLQKVFHRPEGVPLQENGAEFKIEVSILKDKATVMIDTT
                     GSSLFKRGYRAEKGGAPIKENMAAAIIQLSNWFPDKPLIDPTCGSGTFCIEAAMIGMN
                     IAPGFNRDFAFEAWPWVDQSQVQKVRDEAESKANYDIDLDISGFDLDGRMVEIARKNA
                     EEAGLGDVIKLKQMRLQDLKTDKINGVIISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDEGFEKKYGSQADKKRKLYNGTLKVDLYQYYGERVRRQVK"
     gene            complement(36367..36735)
                     /gene="rnpB"
                     /locus_tag="VBC52_000145"
     ncRNA           complement(36367..36735)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VBC52_000145"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(36754..37086)
                     /gene="gpsB"
                     /locus_tag="VBC52_000146"
     CDS             complement(36754..37086)
                     /gene="gpsB"
                     /locus_tag="VBC52_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEO6881755.1"
                     /translation="MASIIYSPKDIFEQDFKVSMRGYDKKEVDVFLDDVIKDYENYLE
                     QIEKLQMENRRLQQALDKKESEASNVRNSGTAMYNQKPIAQSATNFDILKRISRLEKE
                     VFGRQIRE"
     gene            complement(37207..37725)
                     /locus_tag="VBC52_000147"
     CDS             complement(37207..37725)
                     /locus_tag="VBC52_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEO6881756.1"
                     /translation="MKSTILVTGYKNFELGIFQDKDPRITIIKKAIDKDFRRFLENGA
                     DWFIFMGNLGFEYWALEVALDLQKEYDFQIATIFTFENHGQNWNEANKAKLALFKQVD
                     FVKYTFPSYENPGQFKQYNHFLINNTQGAYLFYDSENETNLKFLLEMMEKKEAYDISF
                     LTFDRLNEIYEE"
     gene            37800..38399
                     /gene="recU"
                     /locus_tag="VBC52_000148"
     CDS             37800..38399
                     /gene="recU"
                     /locus_tag="VBC52_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEO6881757.1"
                     /translation="MVNYPHQLIRKTTVTKSKKKKIDFANRGMSFEAAINATNDYYLS
                     HELAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYSGVYKGYYIDFEAKETR
                     QKTAMPMKNFHAHQIEHMANVLQQKGICFVLLHFSTLKETYLLPANELISFYQIDKGN
                     KSMPIDYIRKNGFFVKESAFPQVPYLDIIEEKLLGGDYN"
     gene            38386..40632
                     /locus_tag="VBC52_000149"
     CDS             38386..40632
                     /locus_tag="VBC52_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6881758.1"
                     /translation="MITIKKESVIKLLKYAFGIIMGFIILAIVIGGLLFAYYVSRSPK
                     LTDQALKSVNSSLVYDGNNKLIADLGSEKRESVSADSIPLNLVNAITSIEDKRFFKHR
                     GVDIYRILGAAWHNLVSSNTQGGSTLDQQLIKLAYFSTNKSDQTLKRKSQEVWLALQM
                     ERKYTKEEILTFYINKVYMGNGNYGMRTTAKSYFGKDLKELSIAQLALLAGIPQAPTQ
                     YDPYKNPESAQTRRNTVLQQMYQDKNISKKEYDQAVATPVTDGLKELKQKSTYPKYMD
                     NYLKQVISEVKQKTGKDIFTAGLKVYTNINTDAQKQLYDIYNSDTYIAYPNNELQIAS
                     TIMDATNGKVIAQLGGRHQNENISFGTNQSVLTDRDWGSTMKPISAYAPAIDSGVYNS
                     TGQSLNDSVYYWPGTSTQLYDWDRQYMGWMSMQTAIQQSRNVPAVRALEAAGLDEAKS
                     FLEKLGIYYPEMNYSNAISSNNSSSDAKYGASSEKMAAAYSAFANGGTYYKPQYVNKI
                     EFSDGTNDTYAASGSRAMKETTAYMMTDMLKTVLTFGTGTKAAIPGVAQAGKTGTSNY
                     TEDELAKIEATTGIYNSAVGTMAPDENFVGYTSKYTMAIWTGYKNRLTPLYGSQLDIA
                     TEVYRAMMSYLTGGYSADWTMPEGLYRSGSYLYINGTTTTGTYSSSVYKNIYQNSGQS
                     SQSSSSTSSEKQKEDKNTANDANSSSPQVETPNNGNATTPNNSNQTVPGTGHGNGNGN
                     NNTVPNGN"
     gene            complement(40678..42012)
                     /gene="pepC"
                     /locus_tag="VBC52_000150"
     CDS             complement(40678..42012)
                     /gene="pepC"
                     /locus_tag="VBC52_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEO6881759.1"
                     /translation="MSKLTQTFTDKLFADYQANTKFSAIENAVTHNGLLKSLETRQSE
                     IENDYVFSIDLTKDEVSNQKQSGRCWMFAALNTFRHKLISDFKLENFELSQAHTFFWD
                     KYEKSNWFMEQIIATANQELSSRKVKFLLDVPQQDGGQWDMVVALFEKYGVVPKTVYP
                     ESVSSSASRELNQYLNKLLRQDAQILRELIAQGADGATVQNKKEELLQEIFNFLAMNL
                     GLPPQSFDFAYRDKDNHYQSDKNITPKAFYQKYVNLDLSDYVSIINAPTVDKPYGQSY
                     TVEMLGNVVGGPAVKYLNLDMKRFKELAIAQMKSGETVWFGSDVGQVSNRQKGILATT
                     TYDFNSSMDIKLSQDKAGRLDYSESLMTHAMVLTGVDLDESGQPLKWKVENSWGEKVG
                     KDGYFVASDAWMDEYTYQIVVRKELLTKEELEAYNAEPITLAPWDPMGALAN"
     gene            complement(42125..42946)
                     /gene="nadE"
                     /locus_tag="VBC52_000151"
     CDS             complement(42125..42946)
                     /gene="nadE"
                     /locus_tag="VBC52_000151"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEO6881760.1"
                     /translation="MTLQDQIIKELGVKPVINPSQEIRRSVEFLKDYLLKHSFLKTYV
                     LGISGGQDSTLAGRLAQLAVEELRADTGENYQFIAIRLPYGIQADEEDAQKALDFIKP
                     DIALTINIKEAVDGQVRALNAAGVEITDFNKGNIKARQRMISQYAVAGQYAGAVIGTD
                     HAAENITGFFTKFGDGGADLLPLFRLNKSQGKQLLAELGADKALYEKIPTADLEENKP
                     GIADEIALGVTYQEIDAYLEGKVVSDKSRGIIENWWYKGQHKRHLPITIFDDFWK"
     gene            complement(42943..44403)
                     /locus_tag="VBC52_000152"
     CDS             complement(42943..44403)
                     /locus_tag="VBC52_000152"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEO6881761.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFNKGIHNKRAVFEAYFRKVPFE
                     NGYAVFAGLERIVRYLENLSFSDSDLSYLEELGYPEEFLDYLKNLKMELTVKSAKEGD
                     LVFANEPLVQIEGPLAQCQLVETAILNIINYQTLVATKAARIRSVIEDEPLLEFGTRR
                     AQEMDAAIWGTRAAIIGGANATSNVRAGKIFNIPVSGTHAHALVQTYGDDYQAFKAYA
                     ETHKDCVFLVDTYDTLRVGVPNAIRVAKEMGEKINFLGVRLDSGDLAYLSKKVRQQLD
                     DAGFPNAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     TDAGSMRDTIKLSNNAEKVSTPGKKQVWRITSRAKGKSEGDYITFADTDVTQLDEIEM
                     FHPTYTYINKTVRDFDAVPLLVDIFDKGKLVYQLPSLQEIQEYGRKEFDQLWDEYKRV
                     LNPQDYPVDLARDVWQNKMDLIDRIRKEALAKGEVR"
     gene            complement(44561..45475)
                     /gene="trxB"
                     /locus_tag="VBC52_000153"
     CDS             complement(44561..45475)
                     /gene="trxB"
                     /locus_tag="VBC52_000153"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEO6881762.1"
                     /translation="MYDTLIIGSGPGGMTAALYAARSNLKVGLIEQGAPGGQMNNTAE
                     IENYPGYDHISGPELSMKMYEPLEKFEVEHIYGIVQRVENDGDVKRVITEDESYEAKT
                     VILATGAKNSLLGVPGEEEYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAVFLT
                     QFAKSVTIIHRRDQLRAQKVLQDRAFANEKIKFVWDSVVKEIKGNEIKVSGVTVENLK
                     TGEISEMTFGGVFIYVGLKPHSSMVSELGITDETGWVLTDTNMKTSIPGLYAIGDVRQ
                     KDLRQIATAVGEGAIAGQGVYNYITEHF"
     gene            complement(45544..45768)
                     /locus_tag="VBC52_000154"
     CDS             complement(45544..45768)
                     /locus_tag="VBC52_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEO6881763.1"
                     /translation="MFLEIAKVYLQGLGLASITVILVGIIWVFWRAKKNIDKTARERQ
                     SVLYDVLLIALMTAPILAFAFVAIILMIKA"
     gene            complement(45890..46633)
                     /locus_tag="VBC52_000155"
     CDS             complement(45890..46633)
                     /locus_tag="VBC52_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6881764.1"
                     /translation="MIKLRQLTKSFSGQKVLDKLDLDIEKGQVVALVGASGAGKSTFL
                     RSMNYLEEPDYGTIEIDDFKVDFKSISKDDILTLRRKLAMVFQQFNLFERRTALDNVK
                     EGLKIVKKMSDQEATRIARDELAKVGLADREKYYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKQGQTMVLVSHDMNFVYQVADKVLFLEKGRIL
                     ESGTPEQLFNHPLEERTKEFFASYNKSYL"
     gene            complement(46633..47436)
                     /locus_tag="VBC52_000156"
     CDS             complement(46633..47436)
                     /locus_tag="VBC52_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6881765.1"
                     /translation="MNQFILTGGWSWYNNLVSQVPAGKLFSWKAVLDAIPSILERLPI
                     TLLLTVAGALFGLILALIFAVVKINRVKILYPIQALFVSFLRGTPILVQLMLSYYGIP
                     LFLKFLNQKYGFDWNINAIPASVFAITAFAFNEAAYTSETIRAAILSVDQGEIEAARS
                     LGMTSAQVYRRVIIPNAAVVATPTLINTLIGLTKGTSLAFNAGIVEMFAQAQIMGGSD
                     YRYFERYISVALVYWAVSFLIEQLGNAIERKMAIKAPRHLTDEIPGGVR"
     gene            complement(47531..48343)
                     /locus_tag="VBC52_000157"
     CDS             complement(47531..48343)
                     /locus_tag="VBC52_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6881766.1"
                     /translation="MFKKIILSIATIAATASLAVSVQASEKVELKVATDSDTAPFTYQ
                     KDGKFKGYDVDVVKAVFKGSKYKVTFKTVPFDTISTGIDAGKFDLSANDFSYNKERAE
                     KYLFSDPISRSNYAVVGKKGSHYKSLSDLSGKSTEVLSGVNYAQVLENWNKNHPNKKP
                     IKIKYVSGTTGVTSRLKNIESGKIDFILYDAISSDYIVKDQSLNLSVSPLKGKIGNNK
                     DGLEYLLLPKDKKGKTLQKFINKRIKVLKENGTLARLSKQYFGGDYVSNIDK"
     gene            complement(48481..49824)
                     /locus_tag="VBC52_000158"
     CDS             complement(48481..49824)
                     /locus_tag="VBC52_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6881767.1"
                     /translation="MSFKDFNFKPYIQRALDELKFVDPTDVQAKLIPVVRSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDESSDDVQVVITAPSRELGTQIYQATKQIAEHSEQEIRV
                     VNYVGGTDKLRQIEKLKVSQPHIVIGTPGRIYDLVKSGDLAIHKAHTFVVDEADMTLD
                     MGFLDTVDKIAGSLPKDVQILVFSATIPQKLQPFLKKYLTNPVMEKIKTATVIADTID
                     NWLLSTKGRDKNAQILELSKLMQPYLAMIFVNTKERADELHSYLSSNGLKVAKIHGGI
                     APRERKRIMNQVKNLEFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLSGTAITLYQPSDDSDIRELEKLGINFIPKVIKNGEFQDTYDRDRRNNREKSYQK
                     LDTEMIGLVKKKKKKIKPGYKKKIQWKVDEKRRKERRASNRAKGRAERKAKKQSF"
     gene            complement(49922..50932)
                     /locus_tag="VBC52_000159"
     CDS             complement(49922..50932)
                     /locus_tag="VBC52_000159"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEO6881768.1"
                     /translation="MFLSIMAGVIAFVLTVIAIPRFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLIVALLVSLIFSIILSKENSGNLGATFGILSVVLIYGIIGFLDDF
                     LKIFKQINEGLTPKQKMSLQLIAGLIFYFVHVLPSGTSAINIFGFYLEVGYLYAFFVL
                     FWVVGFSNAVNLTDGIDGLASISVVISLITYGIIAYNQTQFDILLIIVIMIGALLGFF
                     VFNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLFIGFVYVFETSSVMLQVAYFK
                     YTKKKTGVGKRIFRMTPFHHHLELGGVSGKGNKWSEWKVDAFLWAIGIFMSAITLAIL
                     YL"
     gene            complement(50934..53192)
                     /locus_tag="VBC52_000160"
     CDS             complement(50934..53192)
                     /locus_tag="VBC52_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6881769.1"
                     /translation="MTFFKKLKKIFLDYVIHIRDRRSPQKNRERVGQNLMILTIFLFF
                     IFIINFVIIVGTDSKFGVNLSKEAKKVYQQSMTVQAKRGTIYDRNGNPIAEDATTYSL
                     YAIISKNYTTATGQKLYVQPSQYEKVASILENKLGMKKNLVLKQLNQKKLFQVSFGSS
                     GSGLSYTKMADIKKTMEKSDIKGIGFSTSPGRIYPNGIFASQFIGFTLPQDDGDGKKL
                     VGNTGLEAALNKVLSGTDGKVTYEKDRSGNVLLGTATTERRAVNGKDIYTTLSEPIQT
                     VLETQMDVFAEKTKGKFASATVVNAKTGEILATSQRPTYNPSTLKGYDKKNLGTYNTL
                     LYDNFFEPGSTMKVMTLASAIDSKHFNSTEVYNSAQYKIADAIIRDWDVNEGLSSGSY
                     MTFPQGFAHSSNVGMVTLEQKMGRDKWLNYLSKFKFGYPTRFGMLHESGGLFPSDNEV
                     TIAMSSFGQGIGVTQVQMLRAFTSISNDGVMLQPQFISSIYDPNTGTSRTARKEVVGK
                     PVSKEAASKTRDYMVTVGTDPYYGTLYAAGAPVIQVGNQSVAVKSGTAQIAQEGGGGY
                     LQGKNDTINSVVAMVPSENPDFIMYVTIQQPEKFSITFWKDVVNPVLEQATAMKETIL
                     KPGLNDSEHQTKYKLSKIVGENPGHVAEELRRNLVQPIILGNGSKVSKVSKRPGANLA
                     ENEQLLVLTNKLTELPDMYGWSKANVEQFAKWTGIKVTYKGSTSGKVRKQSIDVGKSI
                     NKIKKIKITIGD"
     gene            complement(53196..53522)
                     /gene="ftsL"
                     /locus_tag="VBC52_000161"
     CDS             complement(53196..53522)
                     /gene="ftsL"
                     /locus_tag="VBC52_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEO6881770.1"
                     /translation="MTNEKRTEAVTQTLQRHIKTFSRIEKAFYGAIVITAIIMAVGII
                     YLQSNSLQVKQEVNQLNSKINDKQTEFDNAKQEVNELSNRDRITKIAKDAGLTIQNDN
                     IYRKVD"
     gene            complement(53537..54484)
                     /gene="rsmH"
                     /locus_tag="VBC52_000162"
     CDS             complement(53537..54484)
                     /gene="rsmH"
                     /locus_tag="VBC52_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEO6881771.1"
                     /translation="MTNDFHHITVLLHETVDMLDIKPDGIYVDATLGGAGHSEYLLSQ
                     LGPDGHLYAFDQDQKAIDNAHIRLKKYVDTGQVTFIKDNFRNLSSNLKALGVSEINGI
                     CYDLGVSSPQLDERERGFSYKQDAPLDMRMNREQSLTAYDVVNTYSYHDLVRIFFKYG
                     EDKFSKQIARKIEQVRAEKTISTTTELAEIIKSSKSAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDLLAVDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLQPKMELVNRKPILPSQEELEANNRAHSAKLRVARRIR"
     gene            complement(54567..55820)
                     /locus_tag="VBC52_000163"
     CDS             complement(54567..55820)
                     /locus_tag="VBC52_000163"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEO6881772.1"
                     /translation="MTYIEILGQNAKKASQSVARLSTASKNEILRDLARNIVADTETI
                     LTENARDVVKAKDNGISEIMVDRLRLNKDRIQAIANGIYQVADLADPIGQVVSGYTNL
                     DGLKILKKRVPLGVIAMIFESRPNVSVDAFSLAFKTGNAIILRGGKDAIFSNTALVNC
                     MRQTLQDTGHNPDIVQLVEDTSHVVAEELMQATDYVDVLIPRGGAKLIQTVKEKSKIP
                     VIETGVGNVHIYIDEFADLDMAAKIVINAKTQRPSVCNAAEGLVVHQAIAKGFLSQLE
                     KMLKESNQSVEFRADEEALQLLENAVAASESDYATEFLDYIMSVKVVDSFEQAISWIN
                     KYSSHHSEAIITNNISRAEIFQDMVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKLH
                     ARGPMGLEALTSTKYYINGTGQVRE"
     gene            complement(55830..56633)
                     /gene="proB"
                     /locus_tag="VBC52_000164"
     CDS             complement(55830..56633)
                     /gene="proB"
                     /locus_tag="VBC52_000164"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEO6881773.1"
                     /translation="MKRHFETTRRIVIKVGTSSLVQTSGKINLSKIDHLAFVISSLMN
                     RGMEVILVSSGAMGFGLDILKMDKRPQEISQQQAVSSVGQVAMMSLYSQIFSHYQTHV
                     SQILLTRDVVVFPESLQNVTNSFESLLSMGILPIVNENDAVSVDEMDHKTKFGDNDRL
                     SAVVAKITKADLLIMLSDIDGLFDKNPNIYDDAVLRSHVSEITDDIIKSAGGAGSKFG
                     TGGMLSKIKSAQMVFDNNGQMILMNGANPRDILKVLDGHNIGTYFAQGK"
     gene            56774..58159
                     /locus_tag="VBC52_000165"
     CDS             56774..58159
                     /locus_tag="VBC52_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO6881774.1"
                     /translation="MSREYITLSKNIIKHLGGQNNINNVYHCQTRLRFSLNDPTKVNL
                     EQLKTLKEVKTVVISGGQHQIVIGTHVAKVFEEINSLIETNSTTKIEQTKKAKAVSRI
                     IDFVSGTFQPILPALSGAGMIKALLALLLVFKILTPSSQTYILLNLFADGVFYFLPIL
                     IAITAAQKLKANPILALGTVVMLLHPNWANLVASGKPVSLFHTIPFTLTNYASSVIPI
                     ILIICVQAYIEKYLKQIIPKSLRLVLVPMLIFLSMGILSFSILGPMGTIAGQYLAVIF
                     TFLSKYASWAPAFLVGAFAPILIMFGVHSGIAALGITQLAKLGVDSIFGPGMLCSNIA
                     QATAGTVVTLITKEKKLKEIAGPAAITAYMGITEPILYGVNLPKRYPLIASLIGGGLG
                     GLYAGIMNAHRFAVGSSGLPGLFLYISHTSTHLFITMLIAVIITVSTTAILTFILAQY
                     YEKQVSIRQRD"
     gene            complement(58367..59971)
                     /locus_tag="VBC52_000166"
     CDS             complement(58367..59971)
                     /locus_tag="VBC52_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6881775.1"
                     /translation="MNWSRIWELVKINILYSNPQTLSALRKKQEKHPKKEFSAYKSMF
                     RNQLFQILLFSIIYVFLFVSLDFKEYPGYFTFYIGIFTLVSIIYSFIAMYSVFYESDD
                     VKQYAYLPIKSEELYVAKIFATFGMSVTFLMPILTLMIVAYWRIIGGPLAVLLAIINF
                     AILFLSVTVISLYINSLIGRAIIRSANRKLISTILISLATFGAIVPLLFVNMTSQKMV
                     QGKLQDIAPIPYVRGYYDIVTAPFSMESLLNYYLPLLIILFLIGAIYKWVMPRYYQEL
                     LYGQVKQRKVHRQIDFSKRESINKTLVKHHLSSLQNATLLTNTFLMPLLYLAMFIVPI
                     LNNGKEIGRFFNENYFGIAFLAGILIGSLCVMPASIVGVGISLEKSNFYFIKSLPISF
                     SYFLKHKFVTLITLQLAVPTFIYFLVGFFLLKLSILVLLSFILGLVFMGLIEGQFIYR
                     RDYKHLFLNWQEVTQLFNRGLGQWLLVGSLFGMMIIGSFLIGISIFWSMVWNTVAVNI
                     IILIIGLLILSICQYLLLKNFWKKLV"
     gene            complement(59975..60709)
                     /locus_tag="VBC52_000167"
     CDS             complement(59975..60709)
                     /locus_tag="VBC52_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6881776.1"
                     /translation="MIKFEHVSKVYGEKEALSDLTLSVKDGEIFGLIGHNGAGKTTTI
                     SILTSIIDATYGQVYIDDLLLTEHRDQIKKKIGYVPDSPDIFLNLTAEEYWYFLAKIY
                     DVAPEDIEARITKLVDIFELEEQRYNPIESFSHGMRQKVIVIGALLPNPDIWILDEPL
                     TGLDPQASFDLKEMMKEHAKNGKTVIFSTHVLAVAEQLCDRIGILKQGKLIFVGSLGE
                     LKMKYPDKDLETIYLELAGRQASREG"
     gene            complement(60729..61034)
                     /locus_tag="VBC52_000168"
     CDS             complement(60729..61034)
                     /locus_tag="VBC52_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO6881777.1"
                     /translation="MKKECRDFYRQIQHTYNDISVREDAVLSSILLSASNGLIKTSDV
                     PRVAYELTQQLENNEIEKSFESLATVKELKKSAKKLSERYEQNKVTTGLLSKVFGNH"
     gene            complement(61247..63232)
                     /gene="tkt"
                     /locus_tag="VBC52_000169"
     CDS             complement(61247..63232)
                     /gene="tkt"
                     /locus_tag="VBC52_000169"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO6881778.1"
                     /translation="MTFDTIDQLAVNTVRTLSIDAIQAANSGHPGLPMGAAPMAYVLW
                     NKFLNVNPKTSRNWTNRDRFVLSAGHGSALLYSLLHLAGYDLSIDDLKQFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDLVDHYTYTLHGDG
                     CLMEGVSQEAASLAGHLKLGKLVLLYDSNDISLDGPTSQSFTEDVKGRFESYGWQHIL
                     VKDGNDLEAIAAAIEAAKAETDKPTIIEVKTIIGFGAEKQGTSSVHGAPLGAEGITFA
                     KKAYGWEYPDFTVPAEVADRFASDLQARGAKAEEAWNDLFAKYEVEYPELATEYKEAF
                     AGQAETVELKAHDLGSSVASRVSSQQAIQQLSTQLPNLWGGSADLSASNNTMVAAETD
                     FQASNYAGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNAAWQRAVSET
                     DRPTMLVLTRQNLPVLEGTSELAQEGVNKGAYILSEAKGELDGIIIATGSEVKLALDT
                     QDKLESEGIHVRVVSMPAQNIFDEQEASYQEQVLPSAVTKRLAIEAGSSFGWGKYVGL
                     NGLTLTIDTWGASAPGNRIFEEYGFTVENAVSLYKEL"
     gene            complement(63357..64787)
                     /locus_tag="VBC52_000170"
     CDS             complement(63357..64787)
                     /locus_tag="VBC52_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6881779.1"
                     /translation="MRIEALMEKERRVQYRLLSFLRGSPQAIALKLALLETGLSRATL
                     LKYINNLNSYFEQEKVNCRIVYYKDKLFLEEDYNLSNQEVLKALMKDSIKYTILISLF
                     NQRQFTIVGLSQELMVSEATLNRHLAHLNELLAEFDIAISQGKQIGDELQWRYFYYEL
                     FKQLWSYDKCQNMIKKLDLDSLILLIERLAQHTLTREAHQNLGLWFSICHHRLLAMEK
                     ISDNLKPIVKHYQCNAFYKRLDAALVLYMSRFALEYREGEVLATFAFLHSQNILPINT
                     MEYIMGFGGPIIDCVTETIIYFKKESILADETSDQVIYQLGQLYSHYYFFKGHILVEQ
                     PDLEQTYRLIDHNMRDKLHHISKKIIANVNRIRPLTEDGCSLLTLHLLELLIFSKNSQ
                     KMPFRIGLDMTGNAVEQSLLEYRIRQHFSGNNSIQVEPYDEGKGFDMVIYQSHSRPYK
                     AKLTYCLNKGASERELQEIDSLIYDN"
     gene            complement(64778..66115)
                     /locus_tag="VBC52_000171"
     CDS             complement(64778..66115)
                     /locus_tag="VBC52_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6881780.1"
                     /translation="MTEKYVNKVIVILGASFAGMTCAQKLRQLNPNWDIVLIDKEIHP
                     DYVPNGLNWYYRHEISGLNQAMWQTEEEQRLQNIRCLFGLKVEKINKEDRELMLSDGS
                     SVYYDQLICAMGSQAESTYIDGADAQGVLTTKTYATSQNAKQVLDKSHKVAVVGAGII
                     GLDIAYSLHESGKAVTLLEAQERPDFRHTDPDMSLPLLDAMAESKLHFFQNQKVEKIT
                     VTREEKLCLRTLTGDTFTVDAVILAVNFRPDSRLLTGLVDLSVDNSVVVNDYFQTSDP
                     NIYAIGDLIWSYFKGLNSAYYMPLINQAIRSAQMLAYHLSGHAVPKLKITRATGSKHF
                     GYYRANIGLTELEAGFYEDTVSVTYFPKEQYDLRIKLIANQKTGHLLGAQLISKENCL
                     ATANQLVQAISCDMTDFDLAFQDFIYTARESEMAYMLHQAAINLYEKRIGLCE"
     gene            complement(66203..66901)
                     /locus_tag="VBC52_000172"
     CDS             complement(66203..66901)
                     /locus_tag="VBC52_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6881781.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKNHNSGWIVITFGWG
                     LAVAIAALVSGNISPAHLNPAVSLAFAIKGDLAWGTAILYMIAQIIGAMLGSLLVYLQ
                     FRPHYEAAENRADILGTFATGPALKDNFSNFLSEVLGTLVLVLTIFAIGKYNMPPGVG
                     TMSVGMLVVGIGLSLGGTTGYAINPARDFGPRLLHALLPMKNKGDSDWTYSWIPIVGP
                     MVGAILAALLFAMM"
     gene            complement(66913..68742)
                     /gene="glpO"
                     /locus_tag="VBC52_000173"
     CDS             complement(66913..68742)
                     /gene="glpO"
                     /locus_tag="VBC52_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEO6881782.1"
                     /translation="MEFSRETRRLALQRMQDRTLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVTNTPAANKVLSAEDVLKREPDLQKEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGAYIASHVKAEDFLFDDNNQIIGVRARDLLT
                     DQVIDIKARLVINTTGPWSDTVRNFSNEGKQIHQLRPTKGVHLVVDRQKLNISQPVYV
                     DTGLNDGRMIFVLPREDKTYFGTTDTDYHGDLEHPTVTKEDVDYLLNIVNKRFPEAEL
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKLSDESFEELIDSVKDYIAHKNHREDVE
                     KAISHVESSTSEKELDPSAVSRGSSFERDDNGLLTLAGGKITDYRKMAEGAMETIINI
                     LDKEYNRKFKLINSKTYPVSGGEINPSNVDSEIEAYAQLGTLSGLSIEDARYIANLYG
                     SNAPKLFALTRQITEAEGLSLVETLSLHYAMDYEMALSPTDFFLRRTNHMLFMRDNLD
                     SLIQPVIDEMAKHYQWSDQDKTFYEEELHETLKDNDLAALKDN"
     gene            complement(68755..70263)
                     /gene="glpK"
                     /locus_tag="VBC52_000174"
     CDS             complement(68755..70263)
                     /gene="glpK"
                     /locus_tag="VBC52_000174"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEO6881783.1"
                     /translation="MSSEEKYIMAIDQGTTSSRAIIFNKKGEKIASSQKEFPQIFPQA
                     GWVEHNANQIWNSVQSVIAGAFIESSIKPGQIEAIGITNQRETTVVWDKKTGLPIYNA
                     IVWQSRQTAPIADQLKQEGHTNMIHEKTGLVIDAYFSATKVRWILDHVPGAQERAEKG
                     ELLFGTIDTWLVWKLTDGLVHVTDYSNAARTMLYNIKELKWDDEILELLNIPKAMLPE
                     VKSNSEVYGKTTPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMN
                     TGEEMQLSQNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLRMIETSSESEGL
                     AQSSTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRD
                     VIDTMQVDSGIDIQQLRVDGGAAMNNLLMQFQADILGIDIARAKNLETTALGAAFLAG
                     LSVGYWESMDELKELNATGQLFQATMNESRKEKLYKGWRKAVKATQVFAQED"
     gene            complement(70378..70641)
                     /locus_tag="VBC52_000175"
     CDS             complement(70378..70641)
                     /locus_tag="VBC52_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEO6881784.1"
                     /translation="MIELLLALLLWNILVFATYAIDKRRAIRGAWRISEKTLMIESLL
                     LGGFGAFLGGEVFRHKTTKWYFRLVWYIGMIIDLAIIWLIWCY"
     gene            complement(70730..70987)
                     /locus_tag="VBC52_000176"
     CDS             complement(70730..70987)
                     /locus_tag="VBC52_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEO6881785.1"
                     /translation="MDPKKIARINELSKKKKTVGLTGEEKVEQAKLREEYIEGFRRSV
                     RHHVEGIKLVDDEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(70999..73038)
                     /locus_tag="VBC52_000177"
     CDS             complement(70999..73038)
                     /locus_tag="VBC52_000177"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEO6881786.1"
                     /translation="MTKDLLLELGLEELPAYVVTPSEKQLGQKMVKFLEDHRLSFETV
                     QTFSTPRRLAVRVKGLADQQTDLTEDFKGPSKKIALDAEGNFSKAAQGFVRGKGLSVD
                     DIEFREVKGEEYVYVTKHETGKSAIDVLASVTEVLTELTFPVNMHWANNSFEYIRPVH
                     TLVVLLDDQALELDFLDIHSGRISRGHRFLGSDTEISSASSYEDDLRQQFVIADAKER
                     QQMIVNQIHAIEEKKNISVEIDEDLLNEVLNLVEYPTAFLGSFDEKYLDVPEEVLVTS
                     MKNHQRYFVVRDRDGKLLPNFISVRNGNAEHIENVIKGNEKVLVARLEDGEFFWQEDQ
                     KLNIADLVEKLKQVTFHEKIGSLYEHMDRVKVISQYLAEKADLSDEEKLAVLRAASIY
                     KFDLLTGMVDEFDELQGIMGEKYALLAGEQPAVAAAIREHYMPTSADGELPETRVGAI
                     LALADKFDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILEAFGWDIPLDELVTNLYG
                     LSFASLDYANQKEVMAFISARIEKMIGSKVPKDIREAVLESDTYIVSLILEASQALVQ
                     KSKDAQYKVSVESLSRAFNLAEKVTHSVLVDSSLFENNQEKALYQAILSLELTEDMHD
                     NLDKLFALSPIINDFFDNTMVMTDDEKMKQNRLALLNSLVAKARTVAAFNLLNTK"
     gene            complement(73042..73683)
                     /locus_tag="VBC52_000178"
     CDS             complement(73042..73683)
                     /locus_tag="VBC52_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6881787.1"
                     /translation="MNTLIVYSHPDFSNPYSFTTILQEKFIELYNEHFPNHQLSILNL
                     YDCVLPEITKEVLLSIWSKQRKGLELTADEIVQAKISKDLLEQFKSHHRIVFVSPMHN
                     YNVTARAKTYIDNIFIAGETFKYTENGSVGLMTDDYRLLMLESAGSIYSKGQYSPYEF
                     PVHYLKAIFKDFMAFDDFCVVRAEGTDILDRQVVLDKMNQDLREAFEAFYSKE"
     gene            complement(73683..74597)
                     /gene="glyQ"
                     /locus_tag="VBC52_000179"
     CDS             complement(73683..74597)
                     /gene="glyQ"
                     /locus_tag="VBC52_000179"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEO6881788.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLKSLEL
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLQTGPVTSE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFTQPEYEHSKYSFEISDQVMLLENFE
                     KFEREAKRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEETRIKLLAEED"
     gene            complement(74910..75452)
                     /locus_tag="VBC52_000180"
     CDS             complement(74910..75452)
                     /locus_tag="VBC52_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEO6881789.1"
                     /translation="MSIIIGLMAAMFIIRLAYLKLSIANEKALRKNGAKEYGVGVSKA
                     ITVLHIIIYFSSVTEAILTKASFNFVSVIGLSLMIFSVFMLHTVTRLLGRIWTVKLMV
                     DKNHQFVDHWLFRVVKHPNYFLNIAPELLGVTLLCHAKYTALFVLPIYAFVIYLRIRE
                     ENLLLKTIIIPNGIKKSRVY"
BASE COUNT        23658 a        15178 c        11959 g        24660 t
ORIGIN      
        1 ataaaatgat aatacaatat taggttcgct taagaactca tttagtatac tataattttt
       61 tatttgttgt caataggttt taaaaaaatc tcagagaaaa ccctgagatt ttttaaatta
      121 tgttacaaag ttaatttcct ttagcttcaa ttaaacctag ttcgccatct tcacgcttgt
      181 aaaggacatt tgtcgtatta tcttctgcat ctgtatagat aaagaaatca tgacctaaaa
      241 gttccatttg caacaatgct tcctcaacat ccattggttt tagattaaca ttcttagtac
      301 gtacaattct ttggcttact gcttcttcat ctggctcagc ctcaaattct gttgtgaaaa
      361 cttgacttgc tggaatcttt tcacgatatt ttttcgcaat tttagtttta tttttacgaa
      421 tttgacgctc aattttatca acaactaagt caattgaccc atacatatct tgtgaaacat
      481 cttctgctcg taaagtaata gaatctatta agattgttac ttcaactttt gcggtcttct
      541 ctctgtatac tttgaggttg actctagtat ctaattcttg tgcttcatta aagtattttt
      601 caactttaga gagtttggtc tcaacatact cacgaatagc ttctgttact tcgatgtttt
      661 caccacgaat actgtattta atcatatgag tacctctttc ttgcgttgtt aacgctttct
      721 atactcttat tataaccgct ttcatgaaaa tttgcaagat tttagcgtgc taatgaaaaa
      781 ctttttatat tttttatacc tttttcttct aatagtttcc tgatttgtgc tattgttgcg
      841 cctgttgtat aaatatcatc aacaattaaa aggttgtccc ctaactcagc ctcatttttt
      901 aatgtaaatt gctgaacttg tttgagtctt tcttctttat tattagccga ttgcttacct
      961 ccatcttttt tagaaagaat atttttgtaa ggtatatttg ccgattgtag aaaagcaatc
     1021 acttgattaa attgcctatt ttggtatcct tcatgactca agggaactgg cactatagtg
     1081 tagcccttat acttttttag agcagctttt atttcttttg taaaaacatc tttcaacaaa
     1141 taatcccctt gaaatttaaa aagagaaaaa tattttttca ttgcttcatt gtatctataa
     1201 agggaaaaat gatttacctc tatacctttt ttaccccaat aaatacaatc ttgacattgt
     1261 ttttggggaa tttgcttgca acatgtttgg caacccatct ccctactaag tgcttcaaaa
     1321 ctacccttgc acgtttgaca aattacgttt tgctttggtt ttagaagcat aagttccaca
     1381 aaagttaatt gcgataaatc tatttcatga cataacaaac aagtcattaa aatcctccta
     1441 tatgattcat gtttttaatc tcctttatag cttggtgcat cgatttactt ttcccatcat
     1501 gaaggaagta aaggagccct tcgggccttt ctaatgctct tccaactctt cctgaaattt
     1561 gtaccaagga acttttcgta aacaaatgat gatttgcttg tataacaaaa acgtcgacgc
     1621 tagggaaagt tactcctctc tctagaatgg tagttgacac taggatagac aatttgttat
     1681 ctcgaaaatc ttgcactaac tttaacctag atgtgctctt acttgaaaca aaaccaattt
     1741 tttccatagg aaaggcttgt cttaaaattt cggtgaatac ttgaccttct gaaatatggg
     1801 ggaaaaataa taacaaagga tatctcgttt gtcgctgttt atttatcaac tgatataatt
     1861 tgggaggtag tttttgtgtc tgcaagcttt tctggatacc acttacccaa accatctcgg
     1921 gtatcactaa cggattggca tgaaatctcc tagctagatg caattttttt agttcttttc
     1981 ttgcaacttt tctttctaac tcagtcgttg aagttgcagt cagaaaaata cttgttccgt
     2041 tttcctttaa ggcctgtttt acaccctggt atagtatcgg gttgtcgaca tacggaaaag
     2101 catcaacttc atcaacaatt agcaaatcaa aagcatgata aaattttaac aattgatgtg
     2161 ttgtggcaat agttaaaggg gatctctgat aagaaggaga ttctccatgc attaatgtta
     2221 tggcacatct aaaatcgttt gaaagtcgct tatacaattc taaacaaaca tcaattctag
     2281 gactggcaat acaaacgctt cctccagtat caattacttt ggcgacagag tggtatatca
     2341 tttctgtttt accagcccct gtcacagcat gaacaagaag attttcctta cttgaaatac
     2401 ctttcaacaa ctcttctgaa acctcatttt gataaggtgt cagttcccct ttccatttta
     2461 aagaattcgt tttagaaaat gttttttgtg ggaaataata taaattttca ttactagtaa
     2521 ctcttccaaa aactatacat tctctacaat aataatttcc agttggtaat tggcaatttt
     2581 tagcaacaga actagagcat cgggtacaaa tatagtcgct tcctttcttt ataaaagatg
     2641 gcaagctaat tgcaattttt ctaagttgct cagataattg tgcttttgtc caaagcctac
     2701 ctaaataatt ttccatatta tttcattcgg atttttttgc taaatttgaa gaatttttgt
     2761 agaatttctc tatgaactat aaaacgataa aatcagacgg aattgtcgaa gaagaaatta
     2821 aaaaatcgcg atttatttgt catctaaagc gagtagagtc cgaagaggag gggcgtaatt
     2881 atattacgca aatcaagaaa gcgcactaca aagccaatca ttcgtgttca gctatggtta
     2941 ttggcgaaaa aggggatata aaacgatcta gtgatgatgg agaacctagt ggtacagcag
     3001 gtatccctat gctaaccgta cttgaaaagc aaggcctgac aaatgttgtt gccgtcgtta
     3061 cacggtattt tggaggtatc aaattaggcg caggggggct tattcgtgcc tactctggta
     3121 gcgtagctaa taccatcaaa gagatcgggg tagtagaagt taaagaacaa attggtattc
     3181 gtattcaatt aacttatcct caatatcaaa cttttgacaa cttcctaaaa gagcaccacc
     3241 ttcaggagtt tgaaacagaa tttttagaag ctgtaacctg taaaatatac gttgacccta
     3301 aggaatttga acacacaata actaatttga cagagttcta ccaaggaaaa gctttgttaa
     3361 ctgaagaagg gagtcaaatt gttgaaattc cctttaaggc tgaaaccgat aaataatagc
     3421 tatcacttga taaagaattg atattttact ttaatcaatg tgagttttat aatattctta
     3481 accaatatca tgaagaggta tagcttatgt caaaaattta taattctatt acagacttaa
     3541 tcggaaatac acccattatt caattacatc atattgttcc agaaggagct gcagaggtgt
     3601 acgttaagtt agaatccttt aaccaggttc atctgtcaaa gatcgtatcg ctctagctat
     3661 gattgaagat gcagagcaaa aagggattct taaagccgga gatacaatcg tagaacctac
     3721 atctggtaat acaggtatcg gtttggcttg ggtaggaaaa gctaaagggt ataatgttat
     3781 tattgttatg ccagaaacca tgtcaattga acgtcgtaaa atcattcaag cctatggtgc
     3841 tcaattagta ctaacacctg gtagtgaagg gatgaaggga gctattgcta aggcaaaaga
     3901 aatttctgca gagcaaaatg cttggctacc cctgcaattt aataatcaag ccaatccaga
     3961 aatccacgaa aaaacgactg gtagggaaat tatcgagact tttggtgaaa aaggtttaga
     4021 tgcttttatc gcaggtgtgg gaactggtgg gacaattact ggtgtctctc gtgcattaaa
     4081 aaaagtcaat cctgatgtcg ctatttatgc tgtagaagct gatgaatctg caatcctttc
     4141 tggtgagcaa cctgggcctc ataaaataca agggatttct gcaggtttca ttccagaaac
     4201 attggctaca gattcttatg atcatatcat tcgcgttaca tctgacgatg ctattgaaac
     4261 aggacgtatc atcggcggac tagaaggttt cctcgcagga atttctgctt cagctgctat
     4321 ttatgctgct attgaagtgg ccaaacaact aggaaaaggg aaaaaggttt tagctcttct
     4381 ccctgataat ggcgaacgtt acttatcaac aagcctgtat gattttgaat aactttaatc
     4441 ttcagtaaac gataactaaa aaagattaga tatgttgtct gatctttttt agtttatatc
     4501 tctttaaata ctagtaagcc ctatttagtt aaaatattaa aacgacaaat agaaccaagc
     4561 aatttcactt ggttctattg tttttattga tgatttgaaa cataacggtc aaccgttaac
     4621 agggctgcag cgacatcctc tgcactaatt ttgaatggca tttcatgtat tgtttcacct
     4681 tcaattgttg cttgctgacc aacttttaaa agttcttcat atgttgcatc acccaaatgg
     4741 agttctgcaa gtgtagttgg cattccaatc gcttggtaaa gattaatgta gcgatcaatt
     4801 tcttcctttg gtctattttc gaggaaaagt tgcgtcaacg ttccataggc aactttttca
     4861 ccatgggtta agtgatgaat atctccactc aaggcagtaa aaccattatg aatagcatga
     4921 gctgcagcta atcctgcact ttcaaaacct aaaccactaa gtaaggtatt agcttcaata
     4981 acattttcta aagcttttgt aaccactttg gcatcacaag ccgctaaagc ttgtaaacta
     5041 tcattaaata aagtacgctc acatgcttgg gcaattgcca cgcccgctaa agtctgtcta
     5101 ccgccagcca tcgctatccc atttttttgt aagactgctc tcgcttcaac ccaagtagct
     5161 aagccatctg ctatcccaga agctagtaaa cgttttggcg cttgggcaat tacttgagta
     5221 tcaactaaaa ctaagtctgg attctttgaa taaaaaatat atttttcaaa agctccttca
     5281 tctgtataga ttactgataa cgctgatgtt ggggcatctg ttgaagctac tgttggtgca
     5341 atgaccactg gtaaattaac tttatctgca attgcctttg cagagtcaat agtttttcca
     5401 ccaccaagtc caataataag gtcactattc ttttctttcg caacagcaac gacacgattg
     5461 atttcattat cagatgcttc tccgttaaag gaaacacgat gtggtaacaa atcgtcttgt
     5521 agataagata aaaacctttc tccaacgata ttataaacaa catcatcaca aaggataagt
     5581 ggagtctgac ctaaagattt gatatgttca atactttgga ataaagcatc ctttccttgt
     5641 atatagcgcg atggacttgc gaaaactttc attctcaacc tctttcttct ttaaaaaatt
     5701 ttatgttaaa gatacagcct caaaagttgg cgttctctgt taaatatgtt ctgagtgttg
     5761 actggctttc caatcatctg caaagtcgtt aacagctttt gcaatagatg gcatcgcaaa
     5821 gactgattca aaaatatctg gtcccgcggt gattgattga gcaccctgtg ataaggcctt
     5881 cgttacttgt gaagcgtttt taaaactagc tgctaaaatt ttgctagaag aacctgtccg
     5941 ctctattgct tgcgctaatt ccttaataac ttgtgtcgca tcaatatcaa ggttctccat
     6001 tcggttaaag taaggtgcta ggtagtctgc tccagctgat atagccaaaa gaccttgcat
     6061 actggtgtat attgctgtgg ctgtaatatt ataaccttct gcttttaacg ttttaattgc
     6121 tgctaatcca tctggcgtaa ctgggacttt gatataaatg tcatcgtccg tttcttggcg
     6181 tattttagca gcatcatcca atattccttg ataatctttg gccactactt gaacatgcaa
     6241 acttgcttca cgtccaataa tttctcgaac atcacgaatg cgttggaaaa aatgtatatc
     6301 tccttctttc ttagcaatgg tgggatttga agttacacct gctagtggca gtatatgatg
     6361 ccattttttt atagcttcta aatttaaagt gtcaagtaaa aattccataa taatctccta
     6421 aagagcatgt tctgtacgtg caataatatc atcttgagtt gccttagata ggacattaaa
     6481 gaatgcagaa taacctgcta cacgcacaat caaatcgcgg tgtttttcag ggtgcttctg
     6541 agcatcaatc aaagtctcac gtgagacaac attatactgg atatggtaac catgaaggcg
     6601 attgaagaat gttctaagta aggcaattaa tttttgttta tcttcttctt ttgccaaggt
     6661 ttgagggttg actttttggt taaggagtac accgccgaca atctcatcag ttggtaactt
     6721 cgaaactgat ttcaaaacag aggtaggtcc ctttttgtcc atattatgag atggtgaaca
     6781 accttcagct aatggagttc ctgcatgcct accatctgga gttgctaaag tacctcgtcc
     6841 ttggcctaca tttgctgaaa tagaggacgt tcctgaatag cgaataccac cgattggtcc
     6901 acgtccatag cgtgtgttgg gatatttagc aatttcttca acataaacat cataacactc
     6961 acgtactaaa ctatccgcgt agtcatcatc atttccatat tttggtgctt cattgatcaa
     7021 catttgacga atttcttcac cacgcggacc tgcgtaatca ctctgcaatg cttgccatac
     7081 ttctaatgtg gttaggcgtt tttcttcaaa tactaatttt ttaagcgcag caagtgagtc
     7141 tgaaagatta gcgattccaa cttgtagacc agatatgtag tcatatactg caccaccttc
     7201 ttttaagtgc ttacctcgtc caatacaatc atcagttaaa gctgaacaaa gaatatcagg
     7261 tacttctctt tcgagactaa tatcaatagc attttcaaca ataacgctca ttcgagttaa
     7321 gtaacgaagt gttttgtccc aagcttcttt aagttcttta tatgaggtca tctgggtgaa
     7381 atgcccataa gaaggcgcaa atctcttccc agaagccggg tcaataccgt cattcattgt
     7441 aattaataac actttaggga agttgatata gctcatgccg gtacaacgat aaccccattt
     7501 cccaggaact gctgtttcga cacaaccaat cgctgaataa tcataggcat cttcttcgga
     7561 aactcctttt ttaataaatg atggaataat aatttcatca ttattaaaag ctggcatacc
     7621 aaagcctaat ttcataactt caatagcttc attcatgaat gacttgtcta ggttagcatg
     7681 ataacgaacc gttagattag gctgtggtaa gtgtgtctga gcgactgatt ttagtactag
     7741 aaatgataaa ggattaacag catcttcctt atgtcgtgtt tgtcctccaa tcgttacatt
     7801 ttggtaaaga ggactaccag cagacgaaaa cgtatgagct tggctacgaa ccttattaat
     7861 agttattgtt ttaatccata ggttagttag gcgctcaacg atcgaatcct cagtctcacg
     7921 tccagcttct aagtcagatt taacatatgg atacatgtat tgatcaaagc gtccataaga
     7981 tagagaatga ccgtttgatt ctatttgaag aatacactga ataaaccaaa ccgattgaac
     8041 ggcttctgca aacgtttcag ctgggtaata gggcactcgt tcacaaatac tagcgatatc
     8101 taacaattcc tgacgtctct tagcatttgc tgttttagct tgtttttttg ctaggatagc
     8161 aaaacgctct gcataggttt taacagcttc tattgtaatt aaaatagaat catagaaatg
     8221 atatttgtca atactctcag gctttgttaa atctaagtca gctttagctt ttcttgcttt
     8281 tttttcaaag ccaataagcc cttcttctag taacttctga tagttgacag ctaagtgagc
     8341 atccccagaa ttcatcttac cttccatccc aaagaagcct gtctccatat aaacttgaac
     8401 ttcttctgga agcataacgc ccgcacgagc ccgtaaatta ttattttccc agaaaggtgc
     8461 aatatttcta atttgttctt tggtttcttc tgtaatgtaa aagacatcac catctcgttt
     8521 ttcaaaaagg tctagttcat ttacaacaaa ttctagagtg tattctggaa aaataggagc
     8581 atctttatca gatgaagctt ggttaccgac aatcattgtc tcatcgtcaa tatagattgt
     8641 cattttttcc aaaatatttt gcaacatgta agctctcttt aaaacattag gtttttcctg
     8701 atgcttttga taggcttcag ttaccaagat agcacgttct gcatctatga atggtttctt
     8761 atcaagaacc ttatcgcgat aagaatacat gcgctctgtc aaatagccaa aatgcttttg
     8821 agaattttgt tgaacatcat ttgtatattt ttgagtgagt aattgtttgg tcataacagc
     8881 ctccgcttgt ttctttcgaa actaaaaagt ttttcttcgt aaatataata gcacctaaat
     8941 cctaaaataa caagaagaaa ataaagaata gaatgctaca tctaaagttg cacaaatgtg
     9001 catttttaga aaaaggttaa ggtgcattac acacctcaac ctcttatctg ctagctatcc
     9061 cctttccatt tcacattcat ttttgtaagt aatattatct tggattttaa agaatgggaa
     9121 gtaaacagca gttgagatag ctaaaatgac tatttggacg agtgcacctt gccaaccacc
     9181 taccatgaat ccagaaataa ttgctggagt actccatggt aaagtaacac ctgagaatgg
     9241 ttgcataaaa ccaaccgcaa tagctccata aacaattaaa gctgccaaaa ctggtactaa
     9301 aataaaaggc agaaacatca caggattcat tacgattgga aagccaaaga cgattggctc
     9361 gttaacattg aaaattgcgg gaaaagcggc cactttccca agtgccttgt attgttttga
     9421 ttttgctgca aaaagcatcg caataactag tccaaaagtt attcctgagc ccgataaaat
     9481 caagaaacta tccaaaaact gctgtgttac aatgtgagcg ccattatcta atgttaagcg
     9541 atttgctgct aataaagact tattggcatc taaattggat agtaacaaag cagtcacaat
     9601 accattcacc acagattggc catggacacc aaaccaccat aagaatgaaa taaagaaagc
     9661 aatcccaatt gctccataaa gtgagcctgt taagccttgc aacggtactt gaatgatatc
     9721 ataaatcatc tcaataaagg tacctccagt tgttacctta gcaatcaaat aaacaatcat
     9781 cgataaaaga aagataacaa aagctggaat catagcttca aattgttttg ctattgcctg
     9841 tggtacttgc tctggcattt ttataacaat atgatgttgg ataaaccatg tatagatagc
     9901 accaactact aagccaataa taattgctcc aataatcccc tgaccgccaa accaaacctt
     9961 agaaatagca tccgctattg cttctccttt tacaggaata taagatgatt tcattaaaat
    10021 aaagaaagac gaaagtgata aaacgccagc aggtaacggt tctacactac tatttttagc
    10081 ataagcataa gcaattgcaa aacaggaaat caagcccata atcgcaaaag ttcctgaata
    10141 aacttgtata aatggttctg tccattctgg tccaaaaaca ttagctatgg cttgattaag
    10201 acctttaaat ggaagctgcc ctaatattaa aaagagactc ccaacaactg ttagtggtag
    10261 aattgctagc atgccatctt tgagtgcaat aatccctcgc atattgacaa acttcataat
    10321 cggagtaatt attttctgac tatcaaactt tgacatatca agcctctttt cttgtttcca
    10381 ataaactcaa agcaagtttc aaaactttta caccatccaa cataccataa tctgccattg
    10441 gaataactgc tatcggtaca ccgtattcat cacagattga ttttgattta tctagagtat
    10501 agctaacttg tggaccaagt aatgcaacat ctaaatcttt agcataatct gcaatttttc
    10561 cttgtgaata agcttcaata tgtgcctcta tcccttcttt atccgctgcg attttcatat
    10621 tgtttactaa catacctgtt gaaaaacctg ctgcacagaa taaaccaatt tttatcatat
    10681 ttgatcccct ttacttattt ttgaatatgt aattcctggc gtaaatcaat aatttcttta
    10741 atcaagttaa tctcagtaat acttgtcatc aaatgatcct gagaatgaat aaagagtgct
    10801 gagatatctg tccttgtccc tcctgcttct tgtgcaagga attgtgtttg aagattatga
    10861 gcttctagga gtgcactatc tgctagttct atttcaatct tactttctgc aaaatttcct
    10921 tcttttgcaa gttttaaggc ttgatagata tgttgtttag catccccagc attcaaaatg
    10981 agccccataa taatttggtc tgcgactatg atttccataa gcacctccgc tttaatgatt
    11041 ttttataagt ttaaactttc ttacaaaagg ttctagtttt ctttcgcaaa acaaatgtaa
    11101 catgtccctt aatcatcgtc aatagacttt gtgcccttta tgacaatagc gcacctttgt
    11161 gcttattcta taacaattca cttggtaata gaaacattat caaaagagct aggatttatt
    11221 cctagctctt ttgatatata gtatccacaa aggtgtcttt atctaatctt aaaatattta
    11281 aggctgtgga aaaatcagta actaaatgat taatataccc cttttttaaa acggctaaga
    11341 gtgccgcagc cttataattg ccatgagcaa ccgctattct attaggaact ttctgtagtt
    11401 gttctaaggt aattcctatt gtccttttag ctaaatgttg attcacagga tcacctgaat
    11461 gattaaaaaa acgacaagta atttcaccta cagctgcttc ttcatccaag cattgaaaat
    11521 catcttgatt taaaaggtca agccactgtt gttcattgct tttaggttta ccaccaacgc
    11581 ccacaattgc cacatcaagt ttttcccaat tatccattag cccctcaaaa tattttgaag
    11641 ttaatattcc tttcgctaaa ttagcgtttt cttgaactag agttgcatta atgaaagtac
    11701 aacttccttg gaaacgcctt gacatttcgt aaaccagagt attaacgtga tagcgtgcat
    11761 taatatgact tggacctccg gccaaaggaa caaaagaaac ttgagaaacc ggatgaggac
    11821 gcattgcttc aactacttta ctaagtgtcc tcccccaagc aatccccacc ttttcattct
    11881 ctttaatttt ttttcgaatc atagctgcag ctacatgtgc taaatccttc tctatctctg
    11941 aagtatcgct atcttttcgc gaagaaacaa tctcaatatc tttaaggtga tatttacttt
    12001 taaaatagga ttctaactga aaaatttctg gattaaaatc tgaaatttcg attctaacaa
    12061 gaccttcttc tctcgctttg gctaacatac gacttattgt tgtacgataa attcctaatt
    12121 cattggcaat ctcagattgg cttttccctt ccatatagta aaggtaagct actttagcta
    12181 acaatttttg acgctctaat ttcatactca taacctttca gcatttgctt cagtttaaca
    12241 aaaaggttac atctgtgcaa tttgacatga cactttatct attatcgcca caaatgtgca
    12301 aaatgtaaga aaaatagcct cttttatggt acttgtaata aagattgaat ggtaatctct
    12361 gcttcttgga acaaattagc aatttccgaa tttggatgtt tatctgtaat aacttgtgat
    12421 acagttgaca acggaagctg ttccaccaat gcagtctgat taaacttact agaatctgtt
    12481 aaaataatga cttcatcggc agcattcgcc atctcttgtg caacttcaga acgcattaaa
    12541 tctctaccca taaatcctag ttttggatca aaaccatcag tcccaacaaa agctaacgag
    12601 acatgaaaaa gtgaaatcat ctttttcaag agaggaccta ccgtaacctg tgaacttgat
    12661 tgatactcac caccaagtaa gacaatttgg catgaatcat actctcgtaa atagttagca
    12721 ataaagcaag agttggtcaa aatagtgaca tttcgctttg tctgacagat attttctgct
    12781 aaaagagcac aagtagagcc tgattctatt aagatagtgt cattgtcaga caccatattt
    12841 gcagctaagg cagcaatttc ttttttagtt ttatggttaa aagataacct aacattaagg
    12901 tcatccccac tgtttaaaac tgcataaccg tgctctctat gcaaaagccc tttgccttct
    12961 agtttatcta aatctttacg tatcgttact ttagaaactt gcaacaactc cgagagtgta
    13021 ttaacatcta ttttttggta ctgggatact aaactaataa tattttctaa tctattcatg
    13081 aaacacctcc tttctatttt atcatttttt atttattaaa caagttcaat aaactttcat
    13141 tcgtaattga ctttgttacg tataaaaggt atacttaaaa tgaaattgag gaaaataata
    13201 tgacaaatga aaaaggtatt gtattcaaca ttcaacattt tagtattcac gatggtccag
    13261 gtattcgaac aaccgttttc ttaaagggtt gtcctcttag atgtccttgg tgtgctaatc
    13321 ccgagtcgca aaaaatggtt ccagaaacaa tgagagatgc cattactaat gaaagcgtca
    13381 tcgtgggcga agaaaaatcg gttgatgata ttattgaaga agttttaaaa gatattgatt
    13441 tttacgaaga atctggtggg ggcataacgc tatctggcgg tgaaatattt gcacaatttg
    13501 aatttgccaa ggcaatttta aaacgtgcta aatcactggg aatccacacg gctattgaaa
    13561 caactgctta tactagacat gaacaattca tagatcttat ccaatacgtt gattttattt
    13621 acaccgattt aaaacattac aatagcctca aacaccaaga aaagacgatg gttaagaatg
    13681 cttcaatcat caaaaacatt cattatgctt ttgccaacgg taaaacgatt gtgctacgta
    13741 ttcctgtcat acctaatttt aacgattcat tagaagatgc tgaagaattt gcttgtttat
    13801 ttgatcgcct agatatccgt caagtgcaac tcttaccttt ccaccaattt gggcaaaaca
    13861 aatatcaatt acttaatcgt caatatgaaa tggaagagat cgctgcacta cacccagagg
    13921 atctactcga ttatcaagct atttttagca agtataatat tcactgctac ttttaaatat
    13981 ttaaaaccta gacttgtcat taatcaaatc taggtttttt gtttatttat tattcaagta
    14041 ggctagacct tcctcaaccc aactaggtaa attttcctca agtggtttga aacctatctt
    14101 aagacgattg ttagaaaatc gatgacgatg ctgaatatga tgtctctctt cttcaagggt
    14161 ccgaagcgat aaacttgctt tttgtgtaaa ttcatctaca tctataacct gaacatagac
    14221 ttcatcacct acactaagaa catcataaat attatcaatg taacctgttt taatttcaga
    14281 aatatggata agtccagtcc taccatcctc aagagaaaca aaggctccat aaggcctaat
    14341 gccagtgact gttcccttta acttatcgcc aatcttcatt ttattcttta acctcaatcg
    14401 ttaaaataac aacatcttcc aatggtttgt cttgagaacc agtctctact gccgcaatct
    14461 catcaagtac ctcaaaagaa ctttggtcta ctaattgtcc aaatacactg tggcgacgat
    14521 ctagatgcgg tgtaccacct tgtccagcat acaactcagc aattggtgtt ggccaaccac
    14581 ctcgctctaa ttcctttttc gcataaggaa tttttgtatt ttggacgatg aaaaattggc
    14641 tgccattcgt atttggtcca gcattagcca ttgaaagagc tccgcgaaca ttatataatt
    14701 cctcggaaaa ttcgtcttca aaactttccc catagataga ttcaccaccc atacctgtac
    14761 cagttggatc accaccctga atcataaaat ctggaataat acgatggaag ataataccat
    14821 cataataacc ttgtttagct aaaccaataa agtttgctac cgttttagga gcatgatccg
    14881 ggaagagttt tacagtcata tccccgtgat ttgttttaat gacagccaca ggaccttctg
    14941 tattttcaat atctacttgt ggaaatgtta attctttttc tatcaaacct agttcctcca
    15001 gggcatacaa aatgccatct tcttctactt tttttgtgat aaaatctgct tttttttgag
    15061 cttctgggtg tgacactccc atcgccacag ctagtccagc ataatcaaag agttctaaat
    15121 cattgagctc atctccgaaa accaagatat tctctggctt taagcccaaa tgttcaacaa
    15181 ctttagaaac acctaaagcc ttcgatgttc cttttaacac aacatctgaa gaatgatcat
    15241 gccaacgtat caagcgaaga tgttcagcta acggttcagg tagatgaagg ctatctcctt
    15301 tatcttcaaa tgtccacatc tgataaatat catgttcttt attgaaatct ggattaactt
    15361 cgagatcaga atacacaata tcaatcgcat catttaccaa atcatcacga tctgataaaa
    15421 ctgcctcatg acgaccagct aaaccgtact taataccaac agtgtccgcc catttcttat
    15481 attgctctac atattcagct ggaataggac gatggaagat aatattctta tccttgtcct
    15541 taacgtaagc tccatttaat tttacacaat aatcggcatt taaatcttga acttcttttg
    15601 gaacgccata tcgtgctctt cctgaagcaa tacctacaac gattccttta tctttcaaag
    15661 ctttaaaaac ttttaagatt gatggtggca tataacctgt atctttaaca cgaagcgtgt
    15721 catcaatatc aaaaaacact gcctttattt tttttgcttt atatttagtt tttgcgtcca
    15781 tgactaacca ctttcattaa catagtgcct ctattatatc acttattcat catcttgtgg
    15841 taccaaatgg tgttggaaag catagacaac tgcttgagtt cgatctgcta catttagttt
    15901 tcccaaaata ttagaaacat gagttttaac agtctttaac gagataaata attcatcagc
    15961 aatcctttga ttgtcataac ctttggctaa taaattaagg atatcacgtt cccttgctgt
    16021 taagccctca tgtaaagctg gacatttatc atgagcctta attttcttat ctacttcatt
    16081 ttcaatagct tgttctcccc ttgaaacttt acgaatggca ttaagaatct ctgctgcgct
    16141 tgaggtcttt aacatatagc ctttggcacc tgcttcaata acagggtaaa ttttttcatt
    16201 atccaagtaa gatgttaaga ctaaaatggc agcttctggc caatccttca ataatgctaa
    16261 ggttgcttct acaccgtcca tttcaggcat tactaagtcc ataacaacaa cgtctggacg
    16321 taactccagt gcttttttta tgccttctaa accatttgag gcctcaccta ttacttcaac
    16381 atcagcttgt aagtttaaaa aactttttaa accgagacga accatctcat ggtcatcaac
    16441 aagtacaatt tttattttat ccatttttat cctcactctg atttactaaa ggtaatctta
    16501 tatccatagc aactccttta ccaggctgac tgagaagctg taaatttcct gctaaatctt
    16561 ctactcgatc ttcaatattt tttaacccat aacttaaatc atagacagaa tccatatcaa
    16621 atccaatacc attatcaatc atttttaact gcaattcatt ctctgtttga ttaaggtaaa
    16681 cttcgagacg actggcttgt gaatgcttta aagtattact gataaattct tgaccaattc
    16741 taaaaatatt gtcttcaatc tttttgggta aggttagaat tgattcatga taaacaactt
    16801 caatgtcgct cttatcggtc aattctttca atatcattct gaatccttca gatagggtct
    16861 tattttctaa ttcaactggc ctcaaatgaa gtagcagaat tctcaaatca ttttgtgcat
    16921 gctgaagcat ttcttctact gctaataatt gcgagccaac ctgatcaacg tctagctgag
    16981 aaacattctg tgctatccct gacaaaacca tggaagcagc gaacaaatcc tgactaactg
    17041 tgtcgtgtaa atcacgtgca atacgcttac gttcttgttt cacaatttct tggcttttta
    17101 aaatacgact attttcctta gattgtagac ttgctgtcat taaattcatt tttttagaga
    17161 gccgttgaat attggtcgat atttcagtac catcatcggt aacatttatt gattggttat
    17221 ttaaaatgcg acgtaaatta tggttaattt gacgtttact attatcatct aataaaaacc
    17281 aagttagcaa taaaaggata gttaccgcta aagatagaaa gaaaatagag aaaatcaatt
    17341 gttcaatcag ccacaaacga gacgtttgat agaggtgcac caaattaaca cccaaactat
    17401 caataatgac aaaacaaatg gcaaatataa tgacacttcc atagaagaaa gctaaaaagt
    17461 agtggtgctt tttcatttac gactaacctc aactttccca gcgatagtgt ttacaactaa
    17521 tttgacacgt ttcaaaaggt aatattcttc ctcttgtgac aattttatag attcatttct
    17581 tagatcatac tcctcaaaat caaagtactg tacactaccg taaacagaac taatatcagc
    17641 ctttacggca acgtctaaag gaaccaatac tttagtatca ccaaaaactt tttgaattat
    17701 aataacatta tcctgcccag atacaataac atttgttaaa tcaattgtat cagtcccaga
    17761 gattcttata atattaatat cttcaaaagc ataaaagtca ctttcgtgtt gatcagtacc
    17821 taaccattgg tttcgtgttg tcttaacatc taattgatga ttcttaaatt gaatcaaagc
    17881 ataacgattt tttttcttaa cttgcgagaa atgattgatt agaacataca aaactgcaaa
    17941 aacaacggct gcaataatgt aagggttaag cataaagatt aaaaatagta ataacaaact
    18001 tgtcgttaac agaaaattat gacgactatc gttgttataa aatcttaagg ctaaaagaat
    18061 caaagcgaga ataaaaataa aacttgtcca gtcatcagat aaaattttca ttaatcccat
    18121 aactaagaca acggcttcaa caagtagaaa aaactgaaat tttttcataa taatccttcc
    18181 attactctat atgacgtatt ttatcaaaaa gccctatact ttgcaccatt attgagagaa
    18241 aaatttggca tattggcaga gaacctttct aggcatggtc tctgccatag caaattaaaa
    18301 agaagacttt aacatcttct ttttaatttt attgagttaa tggattgtta gctgttcctg
    18361 tattagttgt actatcactt ggtagtgttg aactacttgt agaagtgcta ctactatcag
    18421 aagtgctagc atccgttgat gagcttacag aatccgaggt agttgttcct gttgagcttg
    18481 aagccgaaga atctattggc gtagatgatg agttattcgt tgttttagaa ccaagaagat
    18541 acagcttaac tttatctcct ctccttaatc caacttctcc tccgtaataa ggatcctgac
    18601 ttgctatctt acttgaagat ggaggtgtta ctactgtttg ataaacgcca ttttgcattt
    18661 gatagaagac aagattatct gcatctaatc cgaggtctgt caaggtttct attacttctc
    18721 caacagtcat acctgttaca tcaggcataa tcattggatc attaacagca accgtaagtg
    18781 ttaatttctt attaccatca ggattaaaag atgttccttc tggaagcgac tgtgctaaga
    18841 ttgtaccagt agtataatca ttattaattt tacgcttaac cttgattaaa gattttttaa
    18901 cacctttgcc ttgaagatct ttgaggatat ctttgtaatt atgttcctta taattaccta
    18961 gagtgaaact cttgtttgga gtggcaatat aaagattaac tttagcccct tctctgcgag
    19021 aacgtcctgc agcagggtct gtttttacga caagtccttc tgaaacacta tcactctcaa
    19081 tattacgaat agctcctact ttcaatccag aattttccaa tgtcattttt gcttgagcta
    19141 ctgttttgtt gctaacatct ggaacacgaa tagtcgtagg acttactaaa attttataag
    19201 caaatacaat tattcctaca acacagagtg caaagaataa tttcatgatt gtaccaagaa
    19261 gtcgatgaga agccttcttt ttagacttcg ttttatcgtt actcgcagaa tcaggagtta
    19321 ttttttctgt tgttttggcc tgtttttgat tacgcaacag ttgttctgtc gttagagatg
    19381 atactgtaga tgttactttg ggtagtgtct tagtactctc agtatcatta aaaacaagtt
    19441 ttggttcacg gtgacgtgtc gagcttagag cagttgataa atcacgtccc atctcataag
    19501 ttgtcttata gcgatctgtt agctttttag ctgttgcttt aatgacaatg ttttccaaag
    19561 cttgtggaac gcttttattt tctgcaagga ttgatggtag tggtttttgg aagtgctgta
    19621 gggcaatagt aactgcacta tccccatcat aaggaatatg gccagttaac atttcaaaaa
    19681 gcatgattcc catggcataa atatcactct gaacagtcgc ttttgaacca cgcgcctgct
    19741 ctggcgataa ataatgaaca ctacctaaca tagaattcgt ttgcgttaaa ctagtttcag
    19801 caaacgctac cgcaataccg aaatctgtaa cttttacagt ccctttttta gttaataaaa
    19861 tattttgagg ttttaaatca cggtgtacga tacctttctg atgtgccagt gacatggctg
    19921 aaaggacttc attcatgatt cgaacaactt cattatttga taggggtgca ttgtcttgaa
    19981 tatacttttt taaatcgaat ccatcaacat attccattac taagaactgc tgtccgtctt
    20041 cttcacctat atcacgaata gcgacaatat taggatgtgt cagctcagcc attgctcgtg
    20101 cttctctctg gaagcgagca accgcaatct gatctgtttg ataattggtt cgcaatactt
    20161 ttattgcgac ctcttcatta tctaaaatta gatctcgagc tagataaaca tccgccattc
    20221 cacctcgtcc gattgattta aggatccgat aacgaccagc aaataatttg ccaatctgaa
    20281 tcattaaacc gcctcacttt caacgtaaac tagtgccaca gtgatattat caagacctcc
    20341 tctatggttt gctagagtga ttaaatcttg attcttatca tctaaggttt tttcttgtgt
    20401 tagtactgta gcaatatcgg cattactcaa catattagtt agtccatcac tattaactac
    20461 aaggtagtca ccttcttcaa gtagatgaac tcctaaatca ggttcgactg gattggcttg
    20521 accaattgat tgcgtaataa tatttttttg aggatgacta gcagcttctt cttctgttaa
    20581 ttggccagct tttaccaatt cattaacaag agaatgatca ctcgtcaata aatgatactc
    20641 tccttgacga acgataccga tacgtgaatc tcctacatgt gcgaaaataa tattatctcc
    20701 tacaatggca actgcttcaa tggtagttcc catacctttg taatcatctg actgacctaa
    20761 ttcatatatt ttgcgattct ctgtttcaat agagacaagc atccaatcac gaatttctga
    20821 taactcagaa aaatctgtct ccgcccaatc acttcctaga tcagttacag tcatctcgct
    20881 agcaatattt cctgcacgat gtcctcccat accatctgct aagatgatta gtgggacgcc
    20941 ggctttattt tcaaattgat taataaaatc ttgattattg gaacgacgtt gcccaatatc
    21001 tgttaaaaga gatatttcca tatatttttt tctgacgatg tcagtttcct cctagacact
    21061 acaagatacg tttgacttgt ccaataaaaa agccatctgt atgatattgt tcagggctta
    21121 tactaataca gcctctcttt acaatatcct cttgtgtatg actcagttct acttgctcaa
    21181 aattggggtg gttttctaag aatttttcaa taacttgaaa attttcttcc tcaaaaatgg
    21241 tacaagtgct ataggttatt ataccacctt tacgtagcgt ttgacaaacg ctagatagga
    21301 tagacaattg aatctcttgg agtgcttcaa agtcttgatt agctttattg tacttaatat
    21361 ctggtttacg gcgaattagc ccgatacctg aacaaggtgc gtctactaaa attttgtcaa
    21421 aagtatcctc aagaaaatac tcatgagctt tacttgcatc taacttttta gtttttatct
    21481 tatctgacag acctaggcgt tttgcatttt ccataaccaa ttccaattta tgatcataca
    21541 aatctaaagc agttactgcc cctgtagtca aatatgaggc gatatgactt gtttttcctc
    21601 ctggtgcact acatgcatcc aatacttggt cattacctga gactttgagt gttggagcaa
    21661 ctaattgact agattcatct tggatagtaa tgaggccatc ttggaaaaat gatgtcgatg
    21721 aaaaatttcc tgagtctgca actaaagctg tctctgcaat atgtgaatct ctaacattta
    21781 aagtttcttt gatagtttgt ttttgcgata agtcagtcac acgaagactg gctttatttc
    21841 gctcaaataa actctccata atagctaaag ctcttgtctc accatattgg tcaattaatt
    21901 tttttacgag ccacactggc atggaataag caacagagta acgtttgttt tggcgtttta
    21961 ttgatgcaat ttcagggagc gtctccgaac ttactcttcg taaaacggca ttaattaact
    22021 tttcagcacc ctttttattg cctcgattct tagcaatagt gacagcatca ttaacaatag
    22081 cgtgatctgg gatgttgtcc agatagagta gttgataaag gctcagtagc aagagatgat
    22141 atacccataa ttcaagctta tcacgatcaa ctataaagtg tgatagatac cattctagcg
    22201 ttatttttct agccacagtt ccatagacaa tttctgttac taaggcacga tctttatctg
    22261 atagtctaga tttttttaaa ctcttattaa gtgcgatatt agaataagct cctttatcaa
    22321 aaacttcctc taaagtcatt aaagctaaac cacgcgcact ctttttccaa tcattcgcca
    22381 aatttatctc ctatttctaa attacgacca acaccattta aaaagtcttt tatatccata
    22441 cgaggtttcc ctgctggttg aactgatttg agttcaatag ctccgtcacc agttgctact
    22501 gtcaatgagt tctttgtctt agcgatcact tgacccggtg agccctttcc ttctgacatt
    22561 gttacttcat aaagtttaaa acgatttccc tccaaaaggg tatgtgcaac tggccatgga
    22621 tacatccctc gaacatggtt aaaaatgtcg cgcgatgatt tattccaatc aattctctct
    22681 tcatctggac taatattagg agaaaaactt acctcttctt cattttgtgg tatcggctta
    22741 atatcacctg atagataacc tggaagcgta tctaataaaa ggtcacgacc aacaacggcg
    22801 agtctatcaa acattgtccc tacattgtct tcatcagtta tctcaacgga agcttttgat
    22861 accatatccc ctgcatccat ctttgcaacc atttccatga tggtaacccc agcttctttt
    22921 tcgccattaa taatggcata atgaattggt gccccaccac gatattttgg caatagagaa
    22981 gcatgtacat taattgcaaa acctaccgat tccaaaagct ttgttggtaa gaattgacca
    23041 aaagcagctg ttacaatacc atctgcacct agggtcatta gttgttctaa ttctggactt
    23101 ccagatagtt tttccggctg atacacagga atgttgtttt ccaaggccac ttcttttaca
    23161 ggggtcattt taatttcttt tttacgacct acagcacgat ctggttgcgt cacaacagct
    23221 aaaacatcat acttaccatc tgctaaaata ccttttaaaa ccgtcgctga aaaatcaggg
    23281 gtccccatga ataataattt tgtcattgct ttttcctcgt ctttcttttc tcaattatat
    23341 catagcacta ttctacatca tattttgcgg ttcgtggtct attgctaagc gaagatctct
    23401 attctccttg tcttgagtca tgtctaataa gcgattcaaa acaagttcaa ggttgtcctc
    23461 aaatcgatac ttgataataa tttggtagtg ataaaggtta tgtgttcttg caattggttt
    23521 tggcgttgga cctaacaact ttactttatc actaaagcct tgcttcaata aacttaatac
    23581 ttcataagat tttcgtatta accactcttc atctttatgc gataatgtta aaccaactgt
    23641 aaaataataa ggaggataac caagttgtcg acggatattc atttcatatt gataaaaagc
    23701 ttcaaaatct tgcttttggg ctaattgaat agcataatga ttaggattat aagtttgaat
    23761 gacaacctct ccttcttttt cagcacgccc agcacgtcca gccacttgag ttaatagctg
    23821 aaaagttcgc tctgatgatc gaaaatcagg taaattcaga gaagtatctg catttagtac
    23881 tccaactaaa gtaacatttg gaaaatccaa tcccttagca atcatctgtg ttcccaataa
    23941 aatatcagct tcatgatttc cgaaacgctt caaaatagac tcatgaccgc ctttttgcct
    24001 tgtcgtatca acatccatcc gtaaaatctt tgcgtctggg ataactttca aaagttcttc
    24061 ataagctttt tgggttccag taccatagta agagatactt ttactattgc aatttggaca
    24121 tgttcttgga ataggctttt caaacccaca ataatgacaa ttcatggtct ttgtagccat
    24181 atgtaaagtc agagaaatgt cacaattagg acattgatca acgtagccgc agtctcgaca
    24241 cataataaaa ctagaataac ctctacgatt gagcataaga accacttgct ctttcttatc
    24301 taatctatct cggattttat ctagtaaata tgatgtgaaa ttactaactt cttgttgacc
    24361 aataaaattt ctaaaatcaa taatttcaac ctgaggaata cgtgctttag gattagcacg
    24421 atgttttaat tctaaaaatt tataaacatc ccgactagct ctagctcggc tttcgatact
    24481 tggagttgct gatcccatta gtaacacagc tttataatat tctgcacgta gcaaggccac
    24541 atcgcgcgca tgataccttg ggttagattc ttgcttataa gttgattcat gttcctcatc
    24601 tataatgatg gctccgatat tttccaaagg agcaaaaatt gctgaacgag ctccaacgac
    24661 aactttagct tgacctgatt ttatctttcg ccactcatca aatttttcac cttctgacaa
    24721 gcccgagtgc ataatagcaa cttgctttcc aaaacgagaa ataaaacgat tcgtcatctg
    24781 gggagttaaa gaaatttctg gaacgagaac aatcgctgtt tttcccaact ttaaaacgtt
    24841 atcaatgata tgaagatata cctctgtctt accagaccct gtaatcccct ccaacaagaa
    24901 gggtttattc tgttccttac caatttggtc aacaactatt ttaactactt tagcttgttc
    24961 ctgatttaaa tccaaaaaat ccgtttttag gattccttta aaataattat cagatctact
    25021 agcttctttt tcaatgactt caatgattcc ttcttctctg aaaaaattaa ccacaggaga
    25081 tgaaaattgt ttgtttaaag ctgtgaggcg ccctggttga ggattttcta acaggaattc
    25141 tttcaactcc agtctctttt tcgctcttgc tgctatttgg ctcttttcta acagagttct
    25201 gttgattttg taaatctttt ctgtttttat tgttttctta tctttggcta aatattgaac
    25261 ttctatgaaa cctttttgaa ttaatctgcc tgcttttttg gcatcttgag aagacaaact
    25321 tgaaaagaca atttccgttt tatgaccaaa tagatcttct ctatcttctg atggtaatgt
    25381 gtctgttgct aacaagagtt tatcatattg cgaattcaaa agacttggta acattgattt
    25441 caaaatagat attttataag aaaagacagt atggcgcatc tgatcagcta agtctagttg
    25501 ttcctgattt aaaacaggtt caaaatctag gacctccgca atatccttag tctctaattc
    25561 atcatcatct ctgaaaccga caacaaagcc ttgcaaaaga cgatttcctc ttccaaaagg
    25621 aacatgaacg cgtactccta cttgtaccaa atcttccaaa tctttaggaa tggcatacga
    25681 aaatggttta tctgtctgca tcagaggaat atcaacaata acctgtgcta attttcttgc
    25741 cataaaccct ccctaattct ctaaaatatg aaaagaggct gatacagaat gtttcaacct
    25801 ctcacttttg acttgcttaa attttttcgc cgtcttcttt ttctttagca atttgctctt
    25861 tgattttacg ttcttcttct tctttagcaa ggcgttcagc ttcgatacga gcgcgaactg
    25921 aggcacgttt tgctgatgga tctgggtgaa ttacaacatt acctgactcg atttcttcta
    25981 aagcgcgaag tgttgatttc acagatttaa aatcttgagt ggctttctca ccagcttcca
    26041 attcatgagc acgcttagct tgtaaaataa ctaaagaata ttttgaagga actttatcca
    26101 acaaagtgtc gatagaaggt tttaacatca taaggcaatt ctcttttcta tttttaactt
    26161 aattttttat cagtttcttt gaccatgttg gtataacgcc caataacacg atccacacga
    26221 taatgttctg cttcaatgac acgtttaaca cgttctgctg ctaatgacac ttgatcattg
    26281 acaacagcat agtcatactc cctcatcaaa gcaatttctt ctttggcacg ttcaatacgt
    26341 tgggcaatca cttctggact atctgtccca cgtcctacta aacgctcttc taattcctct
    26401 aaatctggtg gtgttaagaa aatgaaaaca ccgtccggaa ccttcgattt aacttgaagc
    26461 gctccctgaa cttctatttc aagaaaaaca tcaatccctt tgtctaaagt ctcgttgaca
    26521 taacttaaag gagttccata gtagttacca acatactccg catactcaag catttgacct
    26581 tctttaatca acgcttcaaa ctcttcacgt gtacggaaga aataatcaac tccatcaact
    26641 tcgcctgggc gttgtggacg agtagtcatt gaaacggaat aatcaaattt atggtcaggg
    26701 gttgaaaaaa tttcttgcct aactgtcccc ttaccaacac cagatggtcc tgagaaaaca
    26761 attagcaaac cacgttcaga cattacacac tcctcaaatt aatagatcgg gtgttaagtg
    26821 ttaaaaagac cagaacaatc tggttcttac cttaactatt aacttttata gcaattcata
    26881 aattgctccc taaataaagt ttcttctcat tagtttaaca aaaagaaacc tttttttcaa
    26941 ggtctcctat tattttttat agagtctttt taccccacct attccactac ttgaattagt
    27001 aaaatcctca ggagctctct ctccaatttg gataaaaagc ttgatataag atggtgcctc
    27061 aatgcccttt ttagcatagt attttcctgc tctagagcga cgccaaaaca taaagagcgg
    27121 tatgactaaa tatgcaaata tccctacctc aggtagaaaa taagcgagga taatgatgat
    27181 aaagcgaacc acaactgaac gaatggcaaa agttgtcatg acttttataa cccgctcttt
    27241 caattcacta ggaatggtca ctaaatcata acttgccaaa cgaatccgaa taatgtctga
    27301 tgtgagacca actaataacg ttaagagtcc ataagccata acagcaaaat tatgattctc
    27361 aaaagaaatc ataaatttag taaagagagg aatcaaacaa atacctaggt gttcaatgat
    27421 atcaagagct ataatttcat cattagtctt ttgagcatct aggctatttt gtgccctttc
    27481 gtaccaaaaa ttaaaaacaa caacaaagct aacaataaat aaacctatgt tccgtgtaaa
    27541 gtcaccaata tcacccatcg tagccggagt ttttatctcc aaaactaaaa tcgtcatagc
    27601 aatggccaaa attgcatcac taagcgtatc aaaacgttct ttaaattttt gcatacatac
    27661 atccctctta atacattatg gcatctcgac tggaaaaaaa ctatcacagc caatcctttt
    27721 ctcgtgctat tttaagtgcc tccaaacgat tattaactcc taatttatca aaaatatttg
    27781 acgtgtaatt acgaactgta ccattcgata aaaataaagt gcatgcaatt tctttgctgg
    27841 tcttcccttg agcaattagg gaaagaattt ttatttcttg ctcactcaaa ggattagata
    27901 taacgttttc cattaattca ggcgaatatt ctttaccccc agataaaact ttttgaatcg
    27961 ttttcatcaa atcagctact gagcggtctt tcaaaacata agcatctaca tttgaacgaa
    28021 tagccctttg gaaataacct gaccgcttaa aggttgttac aattatgacc ttaacattct
    28081 ggtattttct aacccactct aagacatcta aacctgataa tatcggcatc tcaacatcca
    28141 atattgcaac atctatggct ttattggata aaatggcaat agcttctcca ccatttccag
    28201 cttgatcaat agtagaaaca ctttcttcca tcaaaagtaa ttggcacata gcatctcgta
    28261 gcatggactg atcctcagca acaagtaatt tcatcttata cctctttcaa taccacctga
    28321 atatgagttg gtttacttcg ggacaaaatc gttagtgtcc cttcaacgat agttaatctc
    28381 tcttgaatac tatgcaactc atctccgtca aggtttgtaa aaccacaacc attatcaatc
    28441 atctcaatat ttatagtagc accttgcctt actaaactca actccacgct atcagcttca
    28501 gcatgcttaa caatattatt ggataattcc tttaatatca ttgtgattgt agattgtgtt
    28561 actggactta gttgtgacgt ctctaactta ttaacaactg ttaacttaat gttagataat
    28621 tgaaacaagc ggtacaactc atcaatttct tccactactg tacgatactt gaggttagaa
    28681 acaatttgac ggacctcact catcgattga tgactaatat gatttaattc tgatagttct
    28741 ttttgtacct tatcatagtt ccttttttct aacaacttta acgctagttc tgtctttagt
    28801 gtcatcatag caaatgtatg tcctaaacta tcatgcaaat cacgccctat accatgcaaa
    28861 tcacgcccta tacggttacg ttcattttca gctgacaaaa tattgatata ctgattttgc
    28921 tctgcaattt tttctctttg ttcttcgcta tttctatttt caatagcaat ccacatgtaa
    28981 ccgatacaaa aaataggaac aattagaaac atgattctat caccaaagtc tgatgcctta
    29041 aagaagcata aaaataccac tgtggctaga gtacaagcaa aactaatgaa tctaaaagac
    29101 ttcaatttat ctctaaagcg atagacgagt aaattactca gaaagtaaat ataccagatg
    29161 aaaccactat ttataaaaat tgtcatgtag aaaatatatg tcaacattgt agaccaggca
    29221 atcatgtgat actttgattc gcccctgata ataacagagt ataaatatgc tcctacaaaa
    29281 gctagcgtaa aaaataaagt ccaaaaaggg tagtgataat agagaatccc tcctatgggg
    29341 taaatcaaaa atactaacgg aataaaatac attaaatcat agttattttt tttatttttc
    29401 aaattgaatg cctcttctga gatagtagat aagcgataac taacattata ataccatagc
    29461 ctatcagaat aataagagaa aaaccagcaa aactattagc aaacggacta aggataactt
    29521 gagtcaaatg gtaagtaggg gttaactttg ataaagcttg caaccatttt gggaacattg
    29581 taattggcat ccacatgcca ccaagaacag ctaatgacat ataacaaata ttggctacca
    29641 ttgacattag ttcagtattt ttaaagtagg agacgagcaa tccaaaagga ataaaacatg
    29701 ttgctcctcc taatagcaat aaaaagctct gcatccattg ttctattggc atactaactt
    29761 gccttaaaat atgcccagtt aggaaaacaa cagttattgc taggcaatag taaaataaaa
    29821 tacgaataat ttttgctaga taatattgcc acattggtac tggtgagtgt cttaataatt
    29881 gcattcggtt tgagagttga tcatctttca tagaaaatgg aaaagtaaaa aaagcaaagc
    29941 taagacttga gaatcctgcc atcgttatca tataattacg aacgtatata gcttcgatgg
    30001 ctttagtgag tccttcgccc ataaatcctg aaaaaaatag aaaaaaagcc actggcatac
    30061 ctatagccat aatgagattg gcaagttgac gttttgtcaa aagaaattcc attttcaata
    30121 atgctttaaa gattctcatg ttttacctcc ttagtagtct caaaaatacg atttaacaaa
    30181 gttctattgg tcatctcaat agcttcaata ggacaaccat tatctgccag tattttccat
    30241 acagcttcag cctccccagt aacaaacgtt atactatcac tcttggcttc acactctgta
    30301 atcaaatagg taggtaataa tttttgataa ctaagcggaa cggtgaataa cttttcggtt
    30361 ttttcttgct tcatggcaaa gggggttgta tcgcgtaata actttccttt atgaagtact
    30421 aaaatcctat cagctgtatg ttctacctct tcgatataat gactagaata gacaattgtg
    30481 acaccttctt tttttagtgt cgcaatcagc tcccaaaatc gttgacgtgt ggaagtatcc
    30541 attccggaag taggttcatc taaaaatagt aattggggct taccaaccaa agttaaaaca
    30601 aaagctaaaa gtcgtttttg ccctccagat aaagtctctg taaattgttg caagtgcgta
    30661 tcactccact gtagatagtc taaaatcaga gacattggca gcacttctgg aaataaacac
    30721 ctttggaaat caatgagttc tctcactgta aactttgagg gcaatacatt ttcttgtggt
    30781 aagatggcca ccttatattt taacctattg tggtgtgcag gcagattaaa aatcgtcaat
    30841 gatcctgaag aaatactaat atcacctaac aaagttgaca tcagagttgt tttccctgca
    30901 ccattaggtc caatcaatgc tacacattgg ttagaagcta tactaaatga tatatcttct
    30961 aaaatcgttt tttgcccgat gcttttaaat atatgatcaa ccttaattat atcttttgcc
    31021 atttggttct cctttactat aatcatagta taaaaaatca gaagtaaact ttttagatat
    31081 ttttgtcatt tcaaaatatg acaaatgtca actctataac aaaaggagag ctaacaagct
    31141 ctccttttga ctattttgca aaatcaacag ctcgcatttc acgaataact gttaccttaa
    31201 tattacctgg ataatccaag ttttgttcga ttttttcacg taccttatgc gacataataa
    31261 ctacttgatc atcagaaact ttaccaggtt gtaccatgat acgaatttca cgtccggctt
    31321 gaagtgcaaa ggcattttgc actccctcaa agccattagc aatttcttct aaatcacgca
    31381 gacgtttgat ataattttcc attgactcat tacgcgcacc tggtcgtgct gaactaaggg
    31441 cgtctgcagc agcaacaatt actgcaataa cgctgtcagg ttctacatca ccatggtggc
    31501 ttgcaatagt attaacaaca atcggatgct ctttgtactt acgagcaaat tccataccaa
    31561 tttcaacgtg actgccttca acttcgcgat caatagcttt ccccatatca tggaggaatc
    31621 cagcccgacg agctaaatca acattttctc ctagttctcc agccaaaata cctgcaagtt
    31681 taccaacttc aactgaatga cgaagtacat tttgaccata tgatgtacgg aactgcaaac
    31741 gtcccataat ttttatcaaa tcaggatgca gatttggtgc accaatctca taagctgctg
    31801 cctcaccata ctcacggata cgttgatcca tttccaaacg atttttttca accaactctt
    31861 caatacgagc tggatggata cgtccatctt gaatcaaaga ttctaaagtc atacgagcaa
    31921 tttcacgacg aatggggtcg aaacctgata agacaacgac ttctggcgta tcatctatga
    31981 taacatcaat acctgtcaaa ctttccagag tacgaatatt acgtccttca cgaccaatga
    32041 tacgtccctt catattatca tcagggagat gaacagttgt aattgtttgt tcagtaacat
    32101 attcaccagc taaacgttgc atagcttggg ctagcaagtc ttttgctatc ttgtttgatc
    32161 gatcttttac ttcacgttct gcttctttaa tacgtgtcgc aatatcatga gctaaatctt
    32221 tttcggtatc agctaaaatg atatctttag cttcttcttg actaagctct gcgatgcgtg
    32281 acaattcttc tacttttttt gtttctaaag tagcaatttc ctgttcgcgc tcattaatat
    32341 gtctagattt atcggttaga ctttgttctt tgctatctaa cattttttct ttgttagata
    32401 gattttcatc tttacgatca agagatgatg cacgatctgt taaacgtgcc tccatctgtt
    32461 tcaactcttg tctatcagat ttaaattcct tttcgatctc ttcacgatat tttctagcct
    32521 cttcttttgc ttctaaaagt aattcttttt ggtgagcttt actttcacgt tccgctgctt
    32581 tacggatgtg ttctgcttca atttctgctt taccacgtaa atcaacggca tcttgttctg
    32641 catttaaaag agttagttct gcagcttctt ttgatgattt cattttcatt gaaatagcca
    32701 cataaccaat aatcaaacca atgagtgcac aaaccattgc taatataata ttaaacatgt
    32761 ttatacctca atttttttct agatttcata tagaaagtcc acttttaagt ttatcataaa
    32821 aaaggcgttt tcacaattta aaattataga catttatgat atgattagaa tgttatttaa
    32881 agggattagg agaaattttc aacatctcct ttagtctcta gaatgtaata caaaaggaga
    32941 aaacttatga caaaagaagt tgtcgtagag agttttgaat tagatcacac catcgttaaa
    33001 gcaccctacg ttcgtttaat ttcagaagaa gttggaccgg tcggagacat cattactaac
    33061 tttgatatcc gccttattca gcctaatgaa aatgctattg atactgctgg tttacatact
    33121 atcgagcacc ttctggcgaa gttaatccgc caacgaatta atggtttgat tgattgttct
    33181 ccttttggtt gtagaactgg ttttcacatg attatgtggg gaaaacagga tgctactgaa
    33241 attgctaaag tcatcaaatc tagtctagag gctattgctg gtggggtcac ttgggaagac
    33301 gttcctggaa caaccattga atcttgtgga aattataaag accacagtct ccactctgct
    33361 caagaatggg cgaaattaat tctttcccaa gggatttctg acaatgcttt tgagcgccac
    33421 attgtctgat ctaataaaaa ataaaagtca acttttggaa ttccaaaagt tgacttatta
    33481 atttaaacaa aggattttgt tctgtatcat agagagcaaa atccttttaa tttttcccta
    33541 atatcctcat agcagtcgta gacatctaaa tgcgaataca aaaaacaccc taatcattag
    33601 actgattttc taacttttag ggtgtagaca aaaaactggt tttgttcttt atattaataa
    33661 tctaaactat cagctcttcc aggagcgtta cctacaaagt aacctgtctt ggcattgata
    33721 gagaacaaat aagtgccatc taatttaaac atattgtaat aaccattacc attgataata
    33781 tttacaggtt caagaatata gttatttcca gagaaatagc ctcgcgcctg tgaagtggcc
    33841 acgattttct ctaatactcc tgggttaaat atccaggcag gatttcctgt atccgcaata
    33901 gcagcattat tatacggtac tcgactacgg ttacgttcca aattacctga tgcagtgcca
    33961 gctgcctgat tatttattgt agcagctgat gaggaagatt gaccgttagt tacgtttggt
    34021 tgttcagtta tttgagtgtc tgaaacctta ttagacgaag cctgttcttc ggattgtttt
    34081 acctgattac gaccagttga aacgatggag tgaagtaaac tatctagact agcattacca
    34141 gtattaagag ttttactatc taaagagtca aaattcgctc catcttttac ctctaatttt
    34201 tcactcttct taccatcaac tacagcaggc gacttaaact tgtcgttaac agctttaaca
    34261 gcatcaattt gacgttttag actatcaaat ttaactttta ctttaccgta gtatttagtt
    34321 ccttttaaag catcaacttt cttttctaat tctgacaatt tatcaaattc gctatttttg
    34381 agttgggttt tcttatcatc aacaaaaaag ttagcataag atttgttgaa attatctaag
    34441 ttctgactag cttttttatc ttcttctgat gaagtcgtcg tagccttaga actagaactt
    34501 tgacttgttg cagtttgaga atttgtcgaa ttattattct tgaaatagtt gtaaccaaag
    34561 attgctacta ttagaagtaa aataagagca atcaatgttc ctacgatacc tccacgcttc
    34621 ttagaagagg atttatactc ctctgaatca gcaatagtcg aatctggttc tgttgcagtt
    34681 ggttctgctt ccgtattcag aggaggaact ggggttacac tattttcttg ttcttgtgaa
    34741 gattcttctt taggtgagac agcattagct tctgtatctt gttcttgaga gactgaatta
    34801 tccaactttt tagaaagctc ttctgcagct aacatcgctg ataattcttc gcgctgtttc
    34861 ttgataaaat catctaaaga tgccgtatca aggtttgcaa aatcgcttga ttttgtctca
    34921 aacttttgtg aagctacttc atcacgatgt tgtttgatgt atttatcaag aatactatct
    34981 tcttcggtaa taccagcctt aatttcagaa tcttttctaa cagcctcttc tactgtcatt
    35041 tctttagcat ctttaaattc aagaccatca gtttcttttt ttggttcaaa aaatgggtgt
    35101 ttttgatctt ctgacatgag ttctccccgt tattttactt gacgtctcac acgttcaccg
    35161 taatattggt acaaatcaac ttttaaagta ccattgtaaa gcttacgttt tttatcagct
    35221 tgtgagccgt attttttttc aaatccttcg tcactagtta ggataaactt gctccaagtt
    35281 tttaaaggcg caaatgtttg tcccatctca ttatacaaaa tgtccactgc tttgtcatcc
    35341 aggagtcgtt caccatatgg tgggttggag ataataacac cgttaatttt atctgttttt
    35401 aaatcttgaa gacgcatttg tttcaatttg ataacatctc caagtcctgc ttcttctgca
    35461 ttttttctag caatttctac catacgaccg tctaaatcaa aacctgaaat atctaaatca
    35521 atatcatagt ttgctttact ctcagcttca tcacgaacct tttgtacttg actttgatct
    35581 acccatggcc acgcctcaaa ggcaaaatca cgattaaaac caggagctat attcattcca
    35641 atcatagcag cttcaataca aaaagtacct gaaccacaag ttggatcaat caatggctta
    35701 tcagggaacc aattagataa ttgaataata gctgctgcca tgttttcttt aattggtgca
    35761 ccaccttttt ctgcacggta gccacgtttg aagagacttg atccagtcgt atcaatcatg
    35821 acagtcgctt tatcttttaa aatagaaact tctattttaa attcagctcc attttcttgt
    35881 aagggaactc cttcaggacg gtgaaagact ttttgaagtt tcttagcaac agctttttta
    35941 gaaatggctt gtacactagg ttcattgtgc aattttgact tgacacactt tgctttagca
    36001 ataggaaatt ttgcccctaa cggtaaataa ttttcccagt caaggccata tacaccctga
    36061 aaaagttcct caaaagtacg tgctggaaac tctcctacaa taatctttat acgatcggca
    36121 gctcgtagcc ataggttcgt ttcaatgatt gttttgatat ctccgtggaa acggactcta
    36181 ccgttctcaa cttggcagtc aattcctagg tttctgattt ctctaccaac aatggcttct
    36241 aaaccagcag cagcagttgc tattaatttg aatgattctt tcatttttcc tcttttacac
    36301 tatcgtattc atcacagtta aaaattggct gagatgttat ctcagccact aacctattat
    36361 gcaattttct ataagccatg ttttgttcca gaaacaacta ggaattgttt ctttcgataa
    36421 tcatctgtct actgcaaagc agtcaggata gattgttctc attccaacta tcccatgccc
    36481 ctaccaaagt ttgggttgct agcttgaggg gtttaccgcg ttccactttc tagatttccc
    36541 tagaaactac gtcactgtgg cactttcaaa gctattcagg catatccaaa gacttagccc
    36601 ttttgcttgc cgtaagatag ctagctatct ccctaggctt attttttcgc ctagcacaaa
    36661 cactactggc atcacagcca gtgctagcat ggactttcct catagtatct caatactacg
    36721 cgattatcca aaaattgcat atttgagaaa tccttattct ctaatttgtc ttccaaaaac
    36781 ttctttctca aggcggctaa tgcgttttaa aatatcaaaa ttggttgcag attgggcaat
    36841 cggtttttga ttatacatag ctgtccctga atttcttaca tttgaagctt cgctttcttt
    36901 tttgtctaaa gcttgttgta gacgtctatt ttccatttgg agcttctcaa tttgctcaag
    36961 ataattttcg taatctttga tgacatcatc cagaaataca tcgacttctt tcttgtcata
    37021 tccccgcata ctaactttaa aatcttgctc aaaaatatct tttgggctat aaataatact
    37081 tgccattcta ttctccaaac cagtcaaatc gttttaatat aagtcctatc ctttatatca
    37141 aagtaaataa tacctaaatt aaatggaaaa atcaagcgta aaacgttctc atatccgaaa
    37201 aaacgatcat tcctcatata tttcatttaa gcgatcaaaa gttaggaaag aaatatcata
    37261 agcttccttt ttctccatca tttctagcaa aaattttaaa ttagtttcat tttcagaatc
    37321 ataaaaaaga tatgcacctt gtgtgttgtt aatgagaaaa tgattgtatt gtttaaattg
    37381 acccgggttt tcatagcttg ggaaagtata tttaacaaag tcaacttgct taaagagtgc
    37441 caacttagct ttattagcct cattccaatt ttggccatgg ttctcaaagg taaaaattgt
    37501 cgcaatttga aaatcatatt ctttttgtaa atcaagagca acttccaaag cccagtactc
    37561 aaaccctaaa tttcccataa aaataaacca gtctgctcca ttttccaaga agcgtctaaa
    37621 atccttatca atagcctttt ttataatagt aatccgagga tccttatcct ggaaaatccc
    37681 taattcaaaa tttttgtaac ctgttacaag aatagttgac ttcatttgtt gtccatccta
    37741 attttatggt ataatagttt tgcttatctt agcattttat ttgtaatgca aggagaaaaa
    37801 tggttaacta tccacatcag cttattcgga aaacaacagt tacaaaatca aagaaaaaga
    37861 aaatcgattt tgccaaccgt ggtatgtcat ttgaagctgc tatcaatgca accaatgact
    37921 actatttgtc tcacgaatta gctgtcattc ataagaaacc aacgccagta caaatcgtca
    37981 aggtagatta tcctaaacgt agtcgtgcta agattgtaga agcttatttt agacaagctt
    38041 ctactactga ttattctggt gtttacaaag gttactatat tgactttgaa gccaaagaaa
    38101 cccggcagaa aactgctatg cctatgaaaa attttcatgc tcaccaaata gagcacatgg
    38161 caaatgtatt acagcaaaaa gggatttgct ttgtcttgct tcatttttcc acacttaagg
    38221 aaacctatct actccctgct aatgagttaa tttcatttta tcagattgat aaaggcaata
    38281 aatcaatgcc tattgattat atcagaaaaa atggattttt cgtaaaggag agtgcctttc
    38341 ctcaagtccc ttacttagat attattgaag aaaaattatt aggcggtgat tacaattaaa
    38401 aaggaatctg ttatcaaact attgaagtat gcttttggca ttataatggg atttattatc
    38461 ttagctattg taataggtgg gctcctattt gcatactacg ttagtcgttc tccgaaatta
    38521 accgatcaag ctttaaaatc cgttaactct agtttggttt atgatggtaa taataaactt
    38581 attgccgatt taggctcaga aaagcgtgaa agtgttagtg cggatagcat tccactaaat
    38641 ttggttaacg ctatcacttc tatcgaagat aaacgtttct ttaaacatag aggtgtcgat
    38701 atttatcgta ttttaggtgc agcttggcat aatcttgtta gtagtaatac gcaaggtggt
    38761 tcaacccttg atcaacagtt gattaaactg gcttactttt ctaccaataa atctgaccaa
    38821 acgttaaaac gtaaatcaca ggaagtttgg cttgcgcttc aaatggagcg taaatacacc
    38881 aaagaagaaa ttcttacttt ctatattaat aaagtttata tgggaaatgg gaattatggt
    38941 atgagaacaa cagctaaatc atactttggt aaagacctaa aggaattatc tattgcacaa
    39001 cttgctttgc tcgctggtat tcctcaagca cctacacaat atgaccctta taaaaaccca
    39061 gaatctgctc aaacaagacg taataccgtt cttcagcaga tgtatcaaga taaaaacatt
    39121 tctaaaaagg aatacgacca agctgttgca actccagtaa ctgatggctt aaaagaatta
    39181 aagcaaaaat ctacttatcc aaaatatatg gataactact taaaacaagt tattagtgaa
    39241 gttaaacaaa aaactggtaa agatatcttt actgctgggc taaaagtgta tactaatatc
    39301 aacactgatg cacaaaaaca actatatgac atctacaaca gtgacactta catcgcttat
    39361 ccaaacaatg aattacaaat agcatctacc atcatggatg cgactaatgg taaagtcatt
    39421 gcacaattag gcgggcgtca tcagaatgaa aatatttcat ttgggacaaa tcaatctgtc
    39481 ttaacagacc gcgattgggg ttctacaatg aaacctatct cagcttatgc acctgctatt
    39541 gatagtggtg tctataattc aacaggtcaa tcattaaacg actcagttta ctactggcct
    39601 ggtacttcta ctcaactata tgactgggat cgtcaatata tgggttggat gagtatgcag
    39661 accgctattc aacaatcacg taacgtccct gctgtcagag cacttgaagc cgctggatta
    39721 gacgaagcaa aatctttcct tgaaaaatta ggcatatact atccagaaat gaactattca
    39781 aatgctattt caagtaacaa cagtagcagt gatgcaaaat atggtgcaag tagtgagaaa
    39841 atggcagcgg cttactcggc ttttgcaaac ggcggaactt actataaacc gcaatatgtt
    39901 aataaaattg aatttagcga tggaaccaat gatacttatg cagcgtctgg tagccgtgcg
    39961 atgaaagaga ctactgccta catgatgacg gatatgctga aaacagtact aacatttggt
    40021 actggtacta aagcagctat ccctggtgtt gcacaagctg gtaagactgg tacttccaac
    40081 tatacggaag atgagttagc taaaattgaa gcaactactg gtatctacaa tagcgccgtt
    40141 ggtacaatgg ctcctgatga aaactttgtc ggctatactt ctaagtacac aatggcaatt
    40201 tggactggtt ataaaaatcg ccttacacca ctttatggta gccaactgga tattgctact
    40261 gaggtttatc gtgcaatgat gtcctaccta actggcggat acagtgcaga ttggacaatg
    40321 ccagaagggc tctatcgaag cggaagctac ttgtatatca atgggacaac tacaaccggc
    40381 acttactctt cttctgtcta taagaatatc tatcaaaatt ctggacagtc aagtcaaagc
    40441 agtagttcaa catcctctga gaaacaaaaa gaggataaga atacagcaaa tgatgccaat
    40501 tcatcatccc cacaagtcga aacacctaat aatggtaatg ctactacccc taataattcc
    40561 aatcaaactg tgccaggaac tggacatggt aatggtaatg gtaacaacaa tacagtacct
    40621 aacggtaatt aaaaaactgg atatattatc cagtttttta atttataatt actattacta
    40681 attagctaat gctcccatcg gatcccacgg cgctaaagta attggctctg cgttgtaagc
    40741 ctctaattct tctttagtaa gcaattcttt acgaacgact atttggtaag tatattcatc
    40801 catccaagca tctgaagcta caaaataacc atccttacca actttctctc cccaagagtt
    40861 ctcaactttc cattttaaag gttgtccaga ttcatcaagg tctactcccg ttaagaccat
    40921 agcatgtgtc attaaacttt cactataatc taggcggccc gctttgtctt ggctaagttt
    40981 aatatccata gatgaattaa aatcataagt cgtagttgct aggataccct tttgacgatt
    41041 tgatacttga cctacgtctg aaccaaacca cacagtttct cctgatttca tttgcgcaat
    41101 cgctaactct ttaaaccgtt tcatatcaag gtttaaatat ttaacagctg gccctccgac
    41161 aacatttcca agcatctcaa ctgtatagga ttgtccataa ggcttatcta cagttggagc
    41221 attgataata gaaacataat ctgataggtc aagattaaca tatttttgat aaaatgcttt
    41281 cggtgtaata tttttatcag actgataatg attgtcttta tcacgataag caaaatcaaa
    41341 agattgcggt ggcaagccaa ggttcattgc taaaaagtta aaaatttctt gaagcaattc
    41401 ttccttttta ttttgaacag ttgccccatc tgctccctgt gctattaatt ctcgtagaat
    41461 ctgtgcatct tggcgtaaga gtttattcaa atattgattt agttcacgac tagcacttga
    41521 tgaaacagat tcaggataaa ctgttttagg aacgacacca tatttctcaa acagagcaac
    41581 caccatgtcc cattgtcctc catcttgttg aggtacatct aataggaatt tcaccttacg
    41641 actactcaat tcctgatttg cagtcgcaat aatttgttcc ataaaccaat ttgatttttc
    41701 atacttatcc cagaaaaatg tatgtgcctg agataattca aaattctcaa gtttaaaatc
    41761 tgaaatcagt ttatgacgaa atgtattcag tgcagcaaac atccagcaac gtcctgattg
    41821 tttctgatta gaaacttcat ctttggtcaa atcaattgaa aagacatagt cgttctcaat
    41881 ctcactttga cgagtctcta gagacttcaa taaaccattg tgagttactg cattttcaat
    41941 agcgctaaat tttgtatttg cttggtaatc agcaaataac ttatctgtaa atgtttgagt
    42001 taattttgac ataaattcct ccttttatac ttagctacta ttataaacat tctgaatatt
    42061 tctgcaagtc tgaaagtaat ttattagaga aacaaaaata gctagaatat tctagctatt
    42121 tttcttattt ccagaaatca tcaaaaattg tgattggaag atgacgttta tgttgaccct
    42181 tataccacca attttcaatg atacctcgag acttgtccga aaccaccttc ccttcaagat
    42241 aagcatcaat ttcttggtag gttactccaa gggctatttc atcagcgatt cctggtttat
    42301 tttcttctaa atctgcggtt ggaatctttt cgtacaaggc tttatcagca ccaagttcag
    42361 ctaataactg tttcccctga cttttattaa gtctaaagag aggtaataag tcagcacctc
    42421 catcaccaaa tttggtgaaa aaacctgtaa tattttctgc agcatgatca gtaccaataa
    42481 ccgctccagc atattgacct gcaactgcat attgactaat cattctttga cgagctttaa
    42541 tatttccttt attaaagtct gtaatctcta ctcctgctgc attaagagct ctaacttggc
    42601 catcaacagc ttctttaata ttaatggtca aagcaatatc tggcttgata aaatctaatg
    42661 ctttttgtgc atcttcttca tcggcctgaa taccatatgg taaacgaata gctataaatt
    42721 gatagttttc ccctgtatca gcacgcaact cttcaactgc tagttgagct agacgtcctg
    42781 ctaaagttga atcttgcccc cctgaaatac ccagtacata ggtctttaga aaactatgtt
    42841 ttaatagata gtcttttaaa aattctacgg aacgacggat ttcttgactt ggattaataa
    42901 caggttttac accgagttct ttgataattt gatcttgcaa agtcatctta cttctccttt
    42961 tgccaaagct tctttacgga tacgatcaat caggtccatt ttattttgcc aaacatcgcg
    43021 tgccaaatca actgggtaat cctgtggatt cagaacacgt ttatactcat cccaaagctg
    43081 atcaaattct ttgcgtccgt actcttgaat ttcttgaaga cttggtaatt gataaactaa
    43141 tttccctttg tcaaagatat ctaccagtaa gggtactgcg tcaaaatcac ggactgtttt
    43201 attgatataa gtataagttg gatgaaacat ttcaatttca tctaattgtg tcacatcagt
    43261 gtctgcaaat gtgatgtaat caccctcaga tttcccttta gctcgactgg tgatacgcca
    43321 aacttgcttt ttcccaggtg ttgatacttt ttcagcattg tttgatagtt taattgtatc
    43381 tcgcatacta ccggcgtcag tctcaataga aacaatttta taaacagcac ctagcgcagg
    43441 ctgatcatag gctgtaatta attttgttcc gacaccccaa acatcaattt tagctttttg
    43501 catttttagg ttgaggattg tattttcatc tagatcattc gaagcataaa tctttgcatt
    43561 aggaaaaccg gcgtcatcta actgctgacg aactttctta gacaaataag ctaaatcacc
    43621 tgaatcaagg cgaactccca agaaattaat cttttcgccc atctcttttg caactcgaat
    43681 agcattaggc acacctacac gaagggtatc ataagtatcc actaaaaaga cgcaatcttt
    43741 atgagtttct gcgtaagctt taaatgcctg gtaatcatcg ccataagttt gaactaaagc
    43801 atgtgcatgc gttcctgata ctggaatatt aaagatttta ccagcacgaa cattgctcgt
    43861 tgcgttagca ccaccaataa tcgctgccct agtaccccaa atggctgcat ccatttcttg
    43921 agcacgtcgt gtcccaaact ctaatagcgg ttcatcttca ataacagaac gaatacgagc
    43981 tgcttttgta gcaacaagtg tctggtagtt gatgatattt aaaatcgctg tctctactaa
    44041 ctgacattgt gctaaaggac cttcaatttg aactaatggt tcattagcaa atacaaggtc
    44101 accttccttg gctgatttga cagtcagttc catttttaaa ttttttaagt aatctaaaaa
    44161 ttcttcagga taacctaact cttctaaata ggataaatca ctatcagaaa agctcagatt
    44221 ttctaaataa cgaacaatac gctctaaacc tgcaaaaaca gcataaccat tttcaaatgg
    44281 aaccttgcga aagtaagctt caaacaccgc tcttttattg tgaatacctt tattaaaata
    44341 aacttgcatc atattgattt gatataaatc agtatgtagt gttaaactat cgtctttata
    44401 catcagacaa tctcctttta atcatgagcc taggataagg ctatatgtct aacattatag
    44461 catatttcta tctcggtagt tcagtttatc accttacttt catgaaaata aaaagaaaga
    44521 ctaatcgttt ttcgatagtc ttttctactt ttagtatttc ttagaaatgc tcagttatat
    44581 agttatagac accctgtcca gcgatagcac cttcaccaac agcagtcgca atctgacgta
    44641 gatctttttg acgaacatcc ccaatagcat atagtcctgg aattgatgtt ttcatatttg
    44701 tatcagttaa gacccagcca gtctcgtccg taatacctaa ttctgagacc atgctagaat
    44761 gtggtttcaa accaacgtaa atgaaaacac ctccgaaagt catctctgag atttctccag
    44821 tctttagatt ttcaacagtt acaccagata ctttaatctc atttccttta atctctttga
    44881 ccactgaatc ccatacaaat tttatttttt cgttagcaaa agctcggtct tgaagtactt
    44941 tttgagctct caattgatca cgtcggtgaa taatagtaac acttttagca aattgagtta
    45001 agaagacagc ttcttcaaca gcagaatcac cgccacccac aactaaaaga tcctgatcac
    45061 ggaaaaatgc accatcacaa accgcacagt aagaaacgcc acgactagta tattcctctt
    45121 cacccggaac tcctaaaagg ctgtttttag ctccggtagc aaggattact gttttggctt
    45181 catacgattc atcttcggtg ataacacgct ttacatcacc atcgttctca actctttgaa
    45241 ctataccata aatatgctct acttcaaatt tttccaacgg ctcatacatc ttcattgata
    45301 attctggacc tgagatatga tcgtagcctg gatagttctc aatttcagca gtattattca
    45361 tctgtccacc tggagcacct tgttcaatta acccaacttt aaggtttgaa cgtgcagcgt
    45421 aaagcgctgc tgtcatacca ccaggacctg aacctattat taaagtatca tacattacta
    45481 tttctccttt gaatcttatg gttttattct atagcaaagc gtctatattt ccaactaatc
    45541 tgactaagct tttatcatta aaatgatagc tacaaatgca aaggcaagta ttggagctgt
    45601 cataagagct atcagaagga catcataaag aacagattgg cgttctctag ctgttttatc
    45661 tatgtttttc ttggcacgcc aaaagaccca aattatacca actaaaataa cagttatgct
    45721 ggcaagccct aaaccttgta aataaacttt tgctatttct aaaaacatct ctatccaaca
    45781 agcgattgtc aaaacatatc gcagctttct tatatactta gcctaactca agaataatta
    45841 aaaatgaaga ttagacattc taaccttcat tatatcaaaa tattttagct tataggtacg
    45901 atttgttata actagcgaag aattcttttg tacgttcttc taaaggatgg ttaaataatt
    45961 gttcaggcgt cccagactct aaaatacgac ctttttccaa aaataaaact ttatctgcta
    46021 cttggtaaac aaaattcata tcatgactaa caagtaccat ggtttgcccc tgcttagcag
    46081 catctgcaat agatttttca acttctccaa ctaattctgg atcaagtgct gatgtcggtt
    46141 cgtctaaaag aagaacatca ggtttcatgg ctaatgcgcg tgccaaagca acacgttgtt
    46201 tctgtccacc tgataaatgt cttggataat atttttcacg atctgcaagt ccgacctttg
    46261 ccagttcgtc tcttgcaatc cgtgttgcct cttgatctga catctttttg acaattttca
    46321 aaccttcttt tacattatcc aaagctgtac gacgctcaaa tagattgaat tgttgaaaaa
    46381 ccatggctaa cttacggcgt aatgttagta tgtcatcttt actgattgat ttaaaatcaa
    46441 ctttaaaatc atcaatttca attgttccat aatcaggctc ttctaaataa ttcatactac
    46501 gaagaaaggt tgattttcct gcacctgaag cacctactag agcaacaact tgaccttttt
    46561 caatatctaa atcaagtttg tctaacactt tttgaccaga aaatgattta gtcaattgtc
    46621 tcaattttat cattagcgta ctcctcctgg aatctcatca gttaaatggc gtggagcctt
    46681 aattgccatt tttcgttcaa tagcatttcc caattgttca attaagaaac taaccgccca
    46741 gtaaacaagt gccacagaga tataacgttc aaaatagcga taatcgctac cacccataat
    46801 ttgtgcttgg gcaaacattt caacaatacc agcattaaaa gctaaagagg ttcccttagt
    46861 tagaccaatt aacgtgttta tcaaagtagg tgttgcgact actgctgcgt ttggaataat
    46921 aacacgacga taaacttgcg ctgaggtcat ccctaaacta cgtgctgcct caatttcacc
    46981 ttgatcaacc gacaaaatcg ccgctcgtat agtttcactt gtataagccg cttcattaaa
    47041 agcaaatgca gtaatagcaa atacagaagc cggtatagca ttaatgttcc aatcaaaacc
    47101 atatttttgg tttaaaaatt tcaagaaaag tggaattcca taataactca gcattaattg
    47161 aacaagtatt ggagtcccac gtaaaaaact aacaaataaa gcttgaatcg gatataaaat
    47221 tttgacacgg ttaattttca ctactgcaaa aatcaaagct aaaatgagtc caaataaggc
    47281 tcctgctact gtcaataata gtgttatggg taatcgctct aaaattgagg gtattgcatc
    47341 taaaacagcc ttccaactaa ataatttccc cgctggaacc tgactaacca aattattata
    47401 ccaagaccat ccacccgtca agataaattg attcattcct tctactttcc ttttctaaac
    47461 tttcttatct ccgttattct atcacgcaaa ctctcacatg taaaaagata aaacttatag
    47521 tctcactgat ttatttatca atgtttgaaa cgtaatctcc accgaaatat tgtttactta
    47581 aacgtgccaa agtaccattt tctttcaaaa ctttaatacg cttatttata aatttctgta
    47641 gagttttacc ttttttatct tttggtaaaa ggaggtattc tagtccatcc ttattattac
    47701 caattttacc tttcaaagga gaaacgctta agtttaatga ttggtctttt acaatatagt
    47761 cggatgaaat ggcatcatat aggataaagt caattttccc actctcaata ttttttaatc
    47821 tgctagtaac accagttgtc ccagaaacat atttgatttt tattggtttt ttattaggat
    47881 gatttttatt ccaattttct agaacctgtg catagttaac gccagataaa acttcggttg
    47941 attttccaga gaggtcactt aatgatttgt aatggctccc cttcttccct actacggcat
    48001 aatttgaacg ggatatagga tctgagaaga gatatttttc tgctctttct ttattgtatg
    48061 aaaaatcatt agctgataaa tcaaatttcc ctgcatcaat acctgttgaa atagtatcaa
    48121 aaggaactgt cttgaaggtt actttgtact tactaccttt aaaaacagct ttgacaacat
    48181 caacatcata acctttgaat ttcccgtctt tttgataagt aaatggtgcc gtgtcagaat
    48241 ctgtagctac tttaagttct actttctctg acgcctgaac tgatactgct aggctggcgg
    48301 ttgcggctat agttgcaata gataatatta tttttttaaa cataagaatt ctcctaaaat
    48361 atcatattac tagtctaaca ctctaaaatc tatctgtata atagtatttc cttatcaggt
    48421 gataaagatt ttatatcaat aaaaaagcct gaaacgagct gatttcagac tttaataatt
    48481 ttaaaaactt tgttttttag ctttgcgctc cgcacgcccc ttagcacgat tacttgctct
    48541 acgctctttg cgacgctttt catcaacttt ccattgaatt tttttcttgt aacctggttt
    48601 tatttttttc tttttctttt tcacaagtcc aatcatttct gtatctaatt tttgatagga
    48661 cttttcacgg ttattacgac gatcacggtc gtatgtatct tgaaattcac cgtttttaat
    48721 gactttagga ataaaattaa tacctagttt ttcaagttca cgaatatcag aatcgtcact
    48781 tggctgatat aaagttatag cagtaccaga taagccatta cggcctgtgc gaccgacacg
    48841 gtggacaaag aaagataggt cttgaggtat ggcatcattg ataacatggc taacaccttc
    48901 aatatcaata ccacgcgctg ctaaatctgt tgcaacgata tattcaaatt cgaggttttt
    48961 aacctgattc ataatacgtt tacgttcacg aggtgcaatc ccaccatgaa ttttagctac
    49021 ttttaagcca ttactagaaa ggtacgaatg taattcgtct gcacgctctt tcgtattaac
    49081 aaatatcatt gcaagatagg gctgcattag tttagagagt tccaaaattt gtgcattttt
    49141 atcacgtcct ttagtggaca aaagccagtt atcgatggta tcagcgataa ctgttgctgt
    49201 tttgattttt tccattacag gattggtcaa atatttcttt aaaaatggct gtaatttttg
    49261 tggaatcgtt gctgaaaaga ctaaaatttg gacatccttg ggtaaagacc ctgcaatctt
    49321 atcaacggta tcaagaaatc ccatatctag agtcatatct gcttcatcaa caacaaaagt
    49381 atgcgcctta tgaatagcta aatcacctga cttcaccaaa tcataaattc gtcctggggt
    49441 tccaatgaca atatgaggtt ggcttacttt caacttttca atttgacgta atttgtcagt
    49501 accaccaaca tagttaacca cccgtatttc ttgctctgaa tgctcagcaa tttgtttagt
    49561 tgcctgataa atttgtgttc ccaactctct acttggtgca gtaataacaa cttgaacatc
    49621 atcactactt tcatctagct tctcaaaaat aggaagtaaa aaggtgtggg ttttaccaga
    49681 accagttttc gattccccca caagatcgcg gcctgaacga acaactggaa tcaatttagc
    49741 ttgaacatca gttggatcga cgaattttaa ctcatcgaga gccctctgaa tgtatggttt
    49801 aaaattaaaa tctttaaatg acatgtctat ttccttacta ttattatgtt cattatacca
    49861 ttaaaggcta caagaaagca aaagggaatc tggcagtcca taccacatcc cctaataaag
    49921 tttaaagata taaaatagca agtgtaatag cgctcatgaa aataccaata gcccataaaa
    49981 aagcatctac tttccattcg ctccatttat tacctttgcc agacacacca cctaattcta
    50041 aatggtgatg aaatggtgtc atgcggaaga tacgtttccc aacaccagtt ttcttcttag
    50101 tatacttaaa gtatgctact tgcaacatca ctgaagacgt ctcaaaaaca tagacaaagc
    50161 cgataaaaag caaggtccat tcctgacgta aggcaattga aatggctgct aacatagcac
    50221 ccaatgcaag tgaaccaaca tctcccataa agactttagc tggtttatga ttaaagacaa
    50281 aaaagccaag taaagcacca atcatgataa cgataatcaa taaaatatca aattgcgttt
    50341 gattataagc aatgattcca taagtaatca aactgataac aaccgaaata gaagctagac
    50401 catcaattcc atctgttagg ttcacagcat ttgaaaaacc aacaacccag aaaagaacaa
    50461 aaaaagcgta taagtatcct acttcaaggt aaaaaccaaa aatatttatc gcagatgtgc
    50521 cacttggtaa cacatggaca aaataaaaaa ttagacccgc aattagttgg agcgacattt
    50581 tttgctttgg agtcaagccc tcattgattt gtttaaaaat tttcaaaaaa tcatctaaaa
    50641 aaccaataat tccatagatg agaacaacag ataaaatacc aaaagttgca cctaaattac
    50701 cactattttc tttagataaa ataatactaa aaataagtga gacaagaaga gcaacaatca
    50761 aaaataccgt tcctcccatt gtcggcgtac ctgctttggc tagatgttgt ttgacatctt
    50821 catgcatttg ttgcccgcca attttcttca attggtaaaa cttaatgaag cgcggaatgg
    50881 caataactgt caggacaaat gctatgacac ctgccattat acttaaaaac atgttaatct
    50941 cctattgtaa ttttgatttt tttaatttta ttgatggatt ttccaacatc aatactttgt
    51001 tttcttactt tacctgaggt tgaaccttta taggtcactt ttatacctgt ccatttagca
    51061 aactgttcaa cgtttgcttt agaccaacca tacatatcag gtaattcagt cagtttattt
    51121 gttaatacta gtagctgttc attctcagca agattagctc ctggtctttt agatacttta
    51181 gaaactttac taccatttcc taaaataatt ggctgaacca gattacgacg taattcttcc
    51241 gctacatgac ctggattttc accaactatt tttgatagtt tatattttgt ctgatgttct
    51301 gagtcattca atcccggttt taatatcgtt tctttcattg ctgttgcttg ttctaaaaca
    51361 ggattaacaa catctttcca gaatgtgata ctaaattttt cgggttgttg gatggtaaca
    51421 tacatgatga agtcaggatt ttccgatggc accatagcca caactgagtt aatagtatca
    51481 ttttttcctt gcagataacc accaccacct tcctgggcga tttgggcagt accagatttt
    51541 actgcaactg attgattccc cacctgaata actggggcac ctgctgcgta taatgtacca
    51601 taataaggat ctgtcccaac agtaaccata taatcacgtg ttttactagc tgcttctttg
    51661 gaaactggtt ttccaacgac ttcttttcgt gctgtacgag aagtccctgt atttggatca
    51721 taaatactgc taataaactg tggttgtaac ataacaccat cattcgagat agacgtaaat
    51781 gcgcgcaaca tttgaacttg cgttacacca ataccttgac caaatgagct catagcgata
    51841 gttacttcat tgtcagatgg gaacaaacct ccactttcat gaagcatccc aaaacgtgtt
    51901 gggtaaccaa atttaaactt agaaagatag tttaaccatt tatctcgtcc cattttttgt
    51961 tccaaggtta ccataccaac gttactggaa tgtgcaaacc cttgtggaaa agtcatataa
    52021 ctaccagatg ataacccttc attgacatcc caatctcgaa taatcgcatc tgcaatttta
    52081 tattgtgcac tgttatacac ttcagtagag ttaaaatgct tagaatcaat tgcagaggct
    52141 agagtcatta ccttcattgt tgaaccaggt tcaaaaaagt tatcatatag tagtgtgtta
    52201 taggtaccca aatttttctt atcgtaacct ttaagggtag aaggattata agtaggacgc
    52261 tgactcgttg ctagaatttc acccgtttta gcgttgacaa ctgtagcact cgcaaatttt
    52321 cctttggttt tttctgcgaa aacatccatc tgagtctcta aaacagtctg aataggctct
    52381 gataaagttg tgtagatatc cttaccatta accgccctac gttctgttgt tgcagtacct
    52441 aaaagaacat tcccactacg gtctttttcg tatgttactt taccatcagt acctgataat
    52501 accttgttaa gtgccgcttc aaggcctgta ttaccaacta atttttttcc atctccatca
    52561 tcctgaggaa gagtaaatcc tataaattgt gaagcaaata tcccatttgg ataaatacgc
    52621 cccggactgg tagaaaaacc aattcctttg atatctgact tttccatcgt tttcttgatg
    52681 tcagccatct tggtataaga caatcctgaa cctgaacttc caaaggaaac ttgaaacaat
    52741 ttcttttgat tgagttgttt taggacaaga tttttcttca tgcccaattt attctctaag
    52801 atactagcta ctttttcata ctgagaaggt tgtacatata gtttttgacc tgtagctgtc
    52861 gtatagttct ttgagataat agcgtagaga ctataggtcg tagcatcctc agcgatagga
    52921 tttccattac ggtcgtaaat tgtcccacgc ttcgcctgta ctgtcatact ttgttgatag
    52981 actttcttgg cttccttgga taaattaacc ccaaatttac tatctgtacc aacgatgatg
    53041 acaaagttga tgataaaaat gaaaaatagg aaaatggtta aaatcatcag attttgacct
    53101 actctttccc tatttttctg cggcgaacgc ctatctctta tatggatcac ataatcaagg
    53161 aagatttttt ttagcttttt aaaaaaagtc acagactagt ccaccttccg ataaatattg
    53221 tcgttttgaa ttgttaaacc agcatcttta gcaattttag taattcgatc acgattagaa
    53281 agctcattta cttcttgttt agcattgtca aattctgtct gtttatcgtt gattttacta
    53341 tttaattgat ttacttcttg tttaacttgt aaactattac tttgcaagta aataataccg
    53401 actgccatga taatagcagt aattacaata gcaccataaa aagctttctc aatacgagag
    53461 aaagtcttga tgtgacgttg taaagtttgt gttactgctt cagttcgttt ttcatttgtc
    53521 ataagtttct ttcttattat cgaatacgac gcgctactct caatttcgct gaatgggcac
    53581 gattgttcgc ttctagttct tcttgacttg gtaagatggg tttacgatta acgagctcca
    53641 tcttaggttg taaatcatct ggtataaaag gaagaccttt aggtacttct actgttgatg
    53701 cttctttaaa aagttgctta gtaagacgat cttctaaaga atgaaaagtg atgacagata
    53761 tacgaccatc aacagctaat aagtccattg cttgttgtat agattcatct gcagctccta
    53821 gttcgtcatt gacttcaata cgaatagctt gaaaaatttg tttagctggg tgaccttttt
    53881 tcttcagttc tttggcagat ttagacgatt tgataatctc agccaattct gttgttgtag
    53941 atattgtttt ttcagctcgt acttgctcaa tttttcgagc aatttgtttt gaaaatttat
    54001 cttcgccata cttaaagaaa atccttacta aatcatgata actataagtg ttcacaacgt
    54061 cataagctgt aagactctgc tctctattca ttcgcatatc aaggggggcg tcttgtttat
    54121 atgagaaacc gcgttcacgc tcgtccagct gtgggcttga tacacctaaa tcataacaaa
    54181 taccattaat ttccgacact cctaatgctt ttaagttaga agatagattt ctgaaattat
    54241 ctttgataaa ggttacttga ccggtatcca catatttctt taaacgtata tgagcattgt
    54301 caatagcctt ctggtcttgg tcaaatgcgt aaaggtgccc atctgggcct aattgtgaaa
    54361 gaagatattc gctgtgtcct gccccaccta aagtagcatc tacatagata ccatccggct
    54421 taatatcaag catatccaca gtctcgtgca aaagcacagt aatatgatga aaatcgtttg
    54481 tcataacttt aattgtacca taaatcctaa actttagaaa agtaaaagag agaccgataa
    54541 atgcaatctc tcctattata ggtcaactat tcccttactt gaccagttcc attaatatag
    54601 tattttgtac ttgttaaagc ttctaatccc atcggtccac gcgcgtgtaa tttttgagtc
    54661 gaaataccaa tctctgctcc gagtccaaaa acgaaaccat ctgtaaaacg tgttgaagca
    54721 ttaacataaa ctgccgctgc atctaccata tcctgaaaaa tttctgccct actgatatta
    54781 ttagtaatga tagcttctga gtgatgacta ctgtatttat taatccagct aattgcttgt
    54841 tcaaaagaat ctactacttt tactgacata atatagtcta aaaattcagt agcataatca
    54901 gactcacttg cagcaacagc attttctaaa agttgcaaag cttcctcatc ggcacgaaat
    54961 tcaactgact ggttcgattc tttgagcatt ttctccaatt gagataaaaa tcccttagca
    55021 atagcttgat gaacaactaa cccttcggca gcattacaga cactagggcg ttgggtttta
    55081 gcattaataa caatcttagc tgccatatct aaatctgcaa attcatcaat atagatatga
    55141 acatttccaa cacctgtttc gataacagga atctttgact tttccttcac cgtttgaatt
    55201 aattttgcac cacctcttgg aattaagacg tccacgtagt ctgttgcctg cattaactcc
    55261 tctgctacaa cgtgactagt atcttcaact aattgtacta tatctgggtt gtggcctgta
    55321 tcttgcaatg tttggcgcat acaattaacc aaggcagtat ttgaaaaaat agcatcttta
    55381 ccaccacgca aaataatagc gttccctgtt ttaaaagcta aactaaaggc atccaccgat
    55441 acattgggac gactttcaaa tatcattgca ataaccccta aaggcactct ttttttaagg
    55501 atttttaaac catctaaatt tgtataacca ctaacaactt gaccaatcgg atctgccaaa
    55561 tctgctacct gataaatacc gtttgctatc gcctgtatgc ggtctttgtt tagtcgtaga
    55621 cggtccacca taatttctga gataccatta tcttttgcct taacaacatc gcgagcattc
    55681 tcagtcaaaa tggtttcagt atcagccact atgtttctcg ctaagtccct gagtatctca
    55741 tttttactag ctgtacttaa tcgtgctact gattgactag cttttttagc attttgccct
    55801 aatatttcaa tatacgtcat aactcaatct cactttcctt gagcaaaata agttccaata
    55861 ttatgcccat ctaacacctt taaaatatca cgaggattgg cgccattcat caatatcatt
    55921 tgtccattgt tatcaaacac catttgtgca cttttaattt tactaagcat gcccccagta
    55981 ccaaatttac ttcctgctcc tccagctgac ttgatgatgt catcggtgat ttcagaaaca
    56041 tgactacgta aaacagcatc atcataaata ttaggatttt tatcaaaaag accatcaata
    56101 tctgacagca taattaataa atctgcttta gtaattttag caacgacagc agagagacgg
    56161 tcattatccc caaacttcgt tttatgatcc atctcatcca cgctgacagc atcattctca
    56221 ttgacgattg gcaaaattcc catagagagc agactctcaa aagagtttgt tacattttgc
    56281 aaactctctg gaaaaaccac aacgtcacgt gttaataaaa tttgggaaac atgagtttga
    56341 tagtgcgaaa atatctgaga ataaagactc atcatggcta cttgtcctac gctagaaact
    56401 gcttgttgtt gtgatatttc ttgaggacgc ttatccattt tcaaaatgtc taaaccaaac
    56461 cccatagcac cagatgatac taaaatcacc tccatgcctc tattcataag actagaaata
    56521 acaaatgcta aatggtctat tttacttaaa ttaattttcc cgcttgtctg tacgagggaa
    56581 ctggttccta ctttaataac aattcttcta gttgtctcaa aatgtctttt cataaaaaaa
    56641 ttatatcata ggccctttag aaatgctatc tgaaaacatc attgaaaatt cacaagcatt
    56701 tccaagtgtt ttcttttatt actatgctat aatctagaat cggaaaacgt tttataagta
    56761 ttaaggagat attatgtcta gagaatacat caccttgtca aaaaatatca tcaaacacct
    56821 tggtggacaa aataatatta acaatgttta ccattgtcaa acacgccttc gctttagtct
    56881 caatgatccg acgaaagtaa accttgagca attaaaaact ttaaaagagg taaaaactgt
    56941 cgttatcagc ggagggcaac atcagatagt gatagggact catgtcgcaa aagtgtttga
    57001 agaaattaac tccctcattg aaactaactc aacgactaaa atagaacaaa ccaaaaaagc
    57061 caaagcagtt agtcgaatta ttgattttgt ttctgggact ttccaaccga ttttacctgc
    57121 tttatcaggc gctggtatga ttaaggcatt gctagcttta ttactagtat tcaaaatctt
    57181 gacaccgtca tctcaaacat atatattact aaatcttttt gcagatggtg ttttctattt
    57241 tttacctatc ttaatcgcca tcacagctgc tcaaaaattg aaagctaatc ctattttagc
    57301 tttgggaact gttgtcatgc tccttcaccc taattgggct aatttagtag cttctggtaa
    57361 accagtatcc ttattccata cgatcccatt cacacttact aactatgcat caagtgtcat
    57421 tcccattatt ctaattattt gtgttcaggc ttatatagaa aaatatttaa agcaaattat
    57481 tcctaaatca ctgcgtttag tgcttgttcc tatgttaata tttctcagca tgggaatttt
    57541 gtcgttctct atattgggac caatgggtac tatcgctggc caatatttag ctgttatttt
    57601 tactttttta tccaaatacg ccagttgggc acctgctttt ctagtaggag catttgctcc
    57661 tatcctaatc atgtttggtg ttcatagtgg aattgctgca ttaggaatca cacaactagc
    57721 gaaattaggg gttgattcta tttttggacc aggcatgctc tgctcaaata tcgcacaagc
    57781 aactgcaggt acagtggtga ctcttataac aaaagaaaaa aaacttaaag aaattgctgg
    57841 acctgctgcc ataacagctt atatgggaat caccgaacct atcttatatg gtgtcaacct
    57901 tccgaaacgt tatcctctaa tagcctcact tattggtggt ggtcttggag gactctatgc
    57961 cggtataatg aacgctcacc gcttcgctgt aggctcttct ggtctaccag gtctgtttct
    58021 ttatatcagt catacttcaa cacatctttt tattaccatg cttattgcag ttatcattac
    58081 tgtaagcaca actgctatat tgacattcat tctagcacaa tattacgaaa aacaagttag
    58141 catcagacaa agagactagg ttatccctag tctctttgtc tgataagata taatagatag
    58201 caataaaaat caaatatttt agagctaaaa ctagcgttat taaacaaagg attacagtgg
    58261 ctgccttcca accgtaaagt ccacaacaag cctttatttt atccgctata actatctcag
    58321 ttattaagca ttattcttac tgtatgattt ttctaaaacc tttttactaa acaagcttct
    58381 tccaaaaatt ttttagcagt aagtattggc aaattgataa tatgagcaac ccaatgataa
    58441 ggattatgat attgacagct acagtattcc ataccattga ccaaaaaata cttattccaa
    58501 taagaaaact acctattatc atcattccaa acaatgaacc aactaacagc cattggccta
    58561 accctctatt aaatagttgc gtaacctctt gccaattcaa gaacaaatgt ttatagtcac
    58621 gtctataaat aaactggcct tcgataagtc ccatgaagac tagacctaaa atgaagctca
    58681 ataaaacgag gattgatagt ttaagtagaa agaatcctac caagaaataa ataaaagtag
    58741 gaactgcaag ttgtaaggtt atcaaagtta caaatttatg cttcaagaaa tacgaaaatg
    58801 atataggcaa actttttata aagtagaaat tacttttttc taaagaaata cctaccccta
    58861 caatgctagc tggcatcaca cataaactac ctattaatat tcctgctaga aaagctatcc
    58921 caaaataatt ttcattaaaa aatcgaccaa tctctttacc attattcaaa attggaacaa
    58981 tgaacattgc tagatataat agtggcatta aaaatgtatt tgtcaataat gtcgcatttt
    59041 gtaaacttga taaatggtgt ttaacaagag ttttattaat tgactcacgt ttactaaaat
    59101 cgatttgacg atggactttt ctctgcttga cctgtccata caacaactcc tgatagtagc
    59161 gtggcataac ccatttataa atagcaccta tcaagaataa aatgattaag agcgggagat
    59221 aatagttgag gagactctcc atactaaatg gcgctgtaac aatatcataa taacctctga
    59281 cataagggat tggagcgata tcttgtaatt tcccttgtac catcttttga ctcgtcatat
    59341 tcacaaataa taagggaact attgctccaa atgtagctag tgaaattaat atagtagaaa
    59401 taagtttccg attagcactt cgaataatgg cacggcctat taaactatta atatacaatg
    59461 aaatcaccgt taccgataaa aataaaatag caaaatttat gattgctagg aggaccgcaa
    59521 gtgggccacc tattatacgc caataagcca caatcattaa ggttaggatt ggcattagaa
    59581 aagttacaga catcccaaat gttgcaaaaa ttttagcaac atagagctct tcagatttaa
    59641 taggcaaata agcatattgt ttaacatcgt cactctcata gaaaacactg tacatcgcaa
    59701 taaaagagta gataatggat actagtgtaa agataccaat gtagaacgtg aagtagcccg
    59761 gatattcttt aaaatcaagt gatacaaaga gaaatacata aattattgaa aagagcaaaa
    59821 tctgaaataa ctgatttcta aacatggatt tataagctga aaattctttt ttaggatgtt
    59881 tttcttgctt ttttcttagt gccgatagag tctgagggtt tgaataaagg atattaattt
    59941 ttacgagttc ccagatacgt gaccaattca tactttatcc ctccctacta gcttgacgtc
    60001 ctgctaattc caaatagata gtttccaaat ctttatctgg atatttcatt tttaactcac
    60061 ctaagctacc gacaaagatt aatttccctt gtttcaaaat acctattctg tcgcataact
    60121 gttctgcaac tgctaagaca tgtgttgaaa aaataactgt ttttccattt ttagcatgtt
    60181 ctttcatcat ttctttcaaa tcaaaagaag cttgagggtc taaaccagtt aacggctcat
    60241 ctagaatcca aatgtcagga tttggaagaa gggcacctat aacaatgact ttttgacgca
    60301 taccatgtga aaaactttca ataggattat agcgttgctc ctctagttca aatatgtcta
    60361 ccaattttgt gatacgtgct tcaatatctt ctggggcaac gtcataaatt ttagctaaaa
    60421 agtaccagta ttcctctgcg gttaaattta agaaaatatc tggcgagtcc ggtacataac
    60481 ctattttttt cttaatttgg tcacgatgtt ctgtcaaaag taggtcatca atatagactt
    60541 ggccataagt tgcatcaata atagatgtta gaatactaat tgtcgttgtt tttcctgctc
    60601 cattgtgacc tattaatcca aaaatctcac catctttaac agagagggtt aaatcactga
    60661 gagcttcttt ttctccgtat actttcgaaa catgttcaaa cttaatcata aaaatctcct
    60721 tttatctcct aatgatttcc aaatacttta gaaagcaatc ccgttgttac cttattttgc
    60781 tcatatctct cagacaattt tttagcagat tttttcaatt ccttaactgt agctaatgac
    60841 tcgaaagatt tttctatctc attattttcc aattgctgag ttaactcata agcaacacga
    60901 ggtacatcgc tagtttttat caaaccattc gatgctgata atagtattga tgatagcact
    60961 gcatcttcac ggacagatat atcattgtaa gtatgctgta tctgccgata aaaatcacga
    61021 cactccttct tcataaacaa acctttctaa aaagataatt aataatagtt gtattatttt
    61081 atatatacaa tagttttatc aattatactg cgctatggaa tgtttgtcaa acgttttcaa
    61141 aaaagagtgc tttccatcac ttatcagtct atcaaaacta ttctcaaatc catcctatat
    61201 cttacaaaaa agaagttgag gtatctttcc caacttcttc atgctcttaa agttctttat
    61261 atagcgatac agcattctca actgtaaagc catactcttc gaaaattctg ttacctggtg
    61321 ctgatgctcc ccaagtatca atagtaagtg ttagcccatt taaaccaaca tatttacccc
    61381 aaccgaagct tgatcctgcc tcaatagcaa gacgttttgt aacagctgat ggaagcactt
    61441 gctcttgata tgaagcctct tgttcatcaa aaatgttttg tgctggcata gatacgacgc
    61501 gaacatggat gccctctgac tcaagtttgt cttgagtatc taaagctaat ttaacttctg
    61561 aacctgttgc aatgatgata ccatcaagtt cacctttagc ttctgataaa atataagcac
    61621 ctttattaac cccttcttgg gctaattctg atgttccttc aagaactggt aagttttgac
    61681 gtgttaaaac aagcatcgtt ggtcgatctg tctctgatac agctcgttgc catgcagcgt
    61741 ttgtttcatt accgtctgcg ggacggataa cgttcaaatt aggcattgag cgcactgatg
    61801 ctaattgttc tattggctca tgagttggcc catcttctcc aacagcaata gaatcatgtg
    61861 tcataacata gacagttggc aagttttgaa gtgcagccat acgtactgct ggtaacaagt
    61921 agtttgagaa gacaaagaac gtacccccat aaacacgagt cccaccgtga agcgcaattc
    61981 cattcattgc agcagccatt gcaaattcac gtacaccaaa ccaaatatta cgcccagcgt
    62041 agttacttgc ttggaaatct gtttctgctg ctaccattgt gttattagaa gctgagaggt
    62101 ctgctgaccc tccccagagg ttcggtaatt gagtcgataa ttgttgaata gcttgttggc
    62161 ttgaaacacg actagccact gatgaaccta ggtcatgagc tttcagttca actgtctcag
    62221 cttggccagc aaaagcttct ttgtattcag ttgctaattc tggatactca acttcatatt
    62281 tagcaaaaag atcattccat gcctcttctg ccttcgcacc acgcgcttga agatctgagg
    62341 caaatcgatc tgcaacttca gcaggaacag taaagtctgg atattcccat ccataagcct
    62401 tcttggcaaa agtaatacct tctgcaccaa gtggcgctcc atgaactgaa gatgttccct
    62461 gtttctctgc gccaaatcca ataatagttt taacttcaat aatggttggt ttatcagttt
    62521 cagctttagc agcttcaatt gctgcagcaa tcgcttctaa atcattacca tctttaacta
    62581 agatatgttg ccaaccatat gattcaaaac gtcctttaac atcttctgta aatgattgag
    62641 atgttggacc atcaagtgag atatcatttg aatcataaag aaggaccaat ttaccaagtt
    62701 ttaagtgtcc agcaagactg gcagcttctt ggctaacacc ctccatcaaa caaccatcac
    62761 catgaagtgt ataagtgtaa tgatcaacta aatcaaagcc tggtttatta aatttagcag
    62821 ccaaatgagc ttcagccatt gccataccaa cagcattagc aataccttgc cctaaaggtc
    62881 cagttgttgc ttctacacca tcagtatggt taacttctgg atgtccaggt gtttttgatc
    62941 cccattggcg aaattgtttt aaatcatcaa ttgataaatc atagccagct aaatgaagta
    63001 ggctataaag aagagctgaa ccatgcccag ctgatagcac aaaacggtca cggtttgtcc
    63061 aattgcgact tgtttttggg tttacattta agaatttatt ccaaagcaca taagccatag
    63121 gcgcagctcc cataggaaga cctgggtgcc cagaatttgc tgcttggata gcatcaatag
    63181 aaagcgtgcg gactgtatta accgctaatt gatcaatggt gtcaaatgtc atgataaaac
    63241 ctttctgttt ttaacaacta aatttaaaat tgttcgtctt tgatattata acataaaata
    63301 cgtaaacgtt ttaacaattt ctggagggat taaaaccatt caattaagta atgggattaa
    63361 ttgtcataaa taagtgaatc tatttcctgt agttctcgct ctgaagcacc tttattaagg
    63421 caataggtta gtttggcttt ataaggtctt gagtgactct gataaataac catatcaaat
    63481 ccctttcctt cgtcataagg ttcgacttga atagaattat taccagagaa atgttgtcga
    63541 atgcgatatt ccaacaatga ctgttccaca gcattacctg tcatatctag accaatccta
    63601 aagggcattt tttgactatt tttcgagaaa ataagcaatt ccaacaaatg taaagtcagc
    63661 aaagaacaac catcttctgt taaaggtcta attcgattaa catttgcaat aatcttcttt
    63721 gaaatatgat gcaacttatc cctcatatta tgatcaataa gtcgatacgt ttgttctaaa
    63781 tcaggttgtt caactaaaat atgaccttta aagaaataat aatggctata taactgacca
    63841 agttggtaaa taacctgatc tgatgtctcg tcagctaaaa tactctcctt tttaaagtag
    63901 atgattgttt cggttacaca atcaataatc gggcctccaa atcccatgat gtattccatt
    63961 gtattgattg gtaaaatatt ttgtgaatgt agaaaagcaa aagttgccag aacttctcct
    64021 tccctatact caagagcaaa acgactcata tacaaaacta aggctgcgtc taaccgttta
    64081 taaaaagcat tacattgata atgtttcaca ataggcttga gattatcaga tattttttcc
    64141 atagcaagga gacgatgatg acaaatacta aaccaaagtc ccagattttg atgagcttct
    64201 cgagttaaag tatgttgagc caagcgctct attaatagaa ttaatgagtc taagtctaat
    64261 ttctttatca tattttggca cttgtcataa gaccatagtt gcttgaataa ttcataataa
    64321 aaataacgcc attgcaattc atcaccaatc tgttttcctt gtgaaatagc aatatcaaat
    64381 tccgctaaca attcattgag atgagcaaga tgacgattta aagtagcctc actaaccatc
    64441 aattcttgag aaagaccaac gattgtgaac tgacgctgat taaataaaga tattaagata
    64501 gtgtatttaa tactatcttt catcagagct ttcaacactt cttgattgga taagttataa
    64561 tcctcttcta aaaataactt atccttataa taaacgattc gacaattaac tttttcttgt
    64621 tcaaagtagc tattaagatt attgatatat ttaagcaaag ttgctcgact taaaccagtt
    64681 tctaataaag ctaacttcag tgctattgcc tgaggagagc ctcttaaaaa agaaagtagc
    64741 cgatattgaa cacgcctttc tttttccatt agagcttcta ttcgcatagc ccaatcctct
    64801 tttcataaag gttgatagct gcttgatgca acatataagc catctcagat tctctagctg
    64861 tataaatgaa atcttgaaaa gccagatcaa aatctgtcat atcacacgat atagcttgaa
    64921 caagctgatt ggcagtagcc aaacaatttt cctttgagat taattgagcc cccaataaat
    64981 ggcccgtttt ttggttagca atcaatttta tccttaaatc atactgttct ttagggaaat
    65041 aagtaacact aacggtatcc tcgtaaaaac cagcttccaa ttcagttaat ccaatgttag
    65101 ctctataata gccaaagtgt ttagatcctg tagcgcgtgt tatttttaat tttggaacag
    65161 catgtcccga taaatggtat gccaacattt gtgccgaacg gattgcttgg ttaattaagg
    65221 gcatataata agcactatta agacctttaa aatacgacca aatcaaatcg ccaatagcat
    65281 agatattagg gtcactcgtt tggaaataat catttacaac aacactatta tcaacactta
    65341 aatcaaccaa cccagttaaa agacgactat ccggacgaaa attaacagct aatatgacag
    65401 catcaactgt aaaggtgtct cctgttagcg ttcgcaaaca aagtttttct tctcgggtta
    65461 cagtaatttt ctcaaccttt tgattttgga aaaaatgtag ttttgattca gccatcgcat
    65521 caagtaaagg aagggacata tcaggatctg tatgcctgaa gtctggtctt tcttgagctt
    65581 ctaacaaagt gaccgccttc cctgattcat gtaaactata agcaatatct aaaccgatga
    65641 tacctgcccc aacaactgct accttatgac ttttatctaa tacctgctta gcattttgac
    65701 tagttgcata agtttttgtt gttaaaacac cttgcgcatc agccccatca atataagttg
    65761 attctgcttg ggagcccatg gcacaaatca attgatcata atagacagaa gaaccatctg
    65821 ataacattaa ttccctatct tctttattaa ttttctcaac ttttagacca aatagacagc
    65881 gtatattctg caaccgttgc tcttcttctg tctgccacat ggcctgattt aaaccactaa
    65941 tttcgtgacg ataataccaa tttaagccat ttggaacata atctggatga atttctttat
    66001 caatcaaaac gatatcccaa ttaggattta attggcgtaa tttctgcgca caagtcatac
    66061 cagcaaaaga agctcctaaa atgacaatta ctttatttac atatttttct gtcataatta
    66121 cttattataa caaaagaagt tatttttgaa aacggtaaca aaaaactgag accataaagt
    66181 cggtctcagc atcaaaaatc tcttacatca tagcaaatag taaggctgct aaaatagctc
    66241 ctaccatagg tcctactatc ggaatccatg aataagtcca atcagaatca cctttgttct
    66301 tcatcggtaa caaggcatgc aataaacgag gaccaaaatc acgtgcagga ttaatagcat
    66361 aacctgttgt tccaccgagc gatagaccaa tcccaaccac taacatacca acactcatcg
    66421 taccaactcc tggaggcata ttgtattttc cgatggcaaa aatagtaagt acaagaacaa
    66481 gtgttcccaa tacttcactt aaaaaattgg aaaaattatc cttcaaagcc ggaccagttg
    66541 caaaagttcc aaggatgtct gctctatttt ctgcagcctc ataatggggt ctaaattgca
    66601 aataaacaag aagtgaacct agcattgcac caataatctg tgcaatcata taaagaatag
    66661 ctgttcccca agctaaatca cctttgatcg caaaagctaa tgacacagct ggattcaagt
    66721 gggcagggct aatattgcca gataccaacg ctgcaatagc tacagcaagt ccccatccaa
    66781 atgtgataac aatccatcca gaattatgat tcttcgtttt aggcaaaaca acacctgcta
    66841 caacaccatt accaagtaag actaagagag ctgttcctaa gaattctcca aaaatatcca
    66901 ttattatctc cttcaattat ccttcaaagc tgccaagtca ttgtctttta gagtctcatg
    66961 taattcttct tcatagaaag ttttgtcttg atcagaccat tgataatgtt tagccatctc
    67021 atcaataaca ggctgaatca aagaatctaa attatctctc atgaaaagca tatggttcgt
    67081 acgtcttaag aagaaatccg ttgggctaag cgccatttca taatccatag catagtgaag
    67141 tgacaacgtt tctaccaagc tcagaccttc tgcttctgta atttggcgtg tcaatgcaaa
    67201 taattttggt gcatttgacc cataaaggtt agcaatataa cgtgcatcct caatacttaa
    67261 accacttagt gtgcccaatt gagcataagc ttcaatttca gaatcaacat tagatgggtt
    67321 aatctctcct cctgaaacag gataggtttt agaattgatt agtttaaact tgcgattata
    67381 ttctttatcc aaaatgttaa taatagtttc catggcacct tctgccattt ttcgataatc
    67441 agtaatttta ccaccagcaa gtgttaatag gccattatca tcacgctcaa aacttgatcc
    67501 tcgagaaaca gctgatgggt ccaattcttt ttcagaagta ctagattcta catgagaaat
    67561 agctttttca acatcctcac gatgattttt atgtgcaata taatctttaa cggaatcaat
    67621 taattcttca aaactttcat cgcttagttt tccactattt cctccattat agtcagaggc
    67681 actattccct gatagtagtg gacgcaaacc tgcccatgaa ctctcaatat catcaatagt
    67741 aagctctgct tctgggaagc gtttgtttac aatattaagg aggtagtcaa catcttcttt
    67801 agttaccgta ggatgttcta agtctccatg gtaatcagta tctgtagttc caaaataagt
    67861 cttatcttca cgtggcaaaa caaaaatcat gcgaccatca ttcaggcctg tatcaacata
    67921 aacaggttga gaaatattta atttttggcg atctacaact aagtgaactc ccttagttgg
    67981 acgtaattga tgaatttgtt taccttcatt ggagaagttg cgaacggtgt cactccaagg
    68041 tccagttgta ttaataacaa gcctagcttt tatatcgata acttggtcag tcaacaaatc
    68101 acgcgcacgt actcctataa tttgattgtt gtcatcaaat aagaagtcct cagctttgac
    68161 atgactggca atataagctc catccctatt agcgcgttta atattttcaa tcactaggcg
    68221 tgcatcgtta ttacggaaat ctaaatagac ccctccgcct agcaaacctt ctttttgtaa
    68281 atcaggctca cgcttaagaa catcttctgc gcttaaaact ttattggctg cgggtgtgtt
    68341 tgtcacacct gctaataaat catataaatc catagccact ttaaggcgaa acatactaaa
    68401 agtagaaccc ggctcatcgt aaaccggtaa aagcattgga tctggcttag gaatgtgtgg
    68461 tgcaatttgc tgcacgactg cacgctctga tactgtatca gaaacaactt ctacatcaaa
    68521 ttgcttcaag taacgaagac caccatggac caattttgtt gagcgactac ttgtcccttc
    68581 tgcaaaatct tgcatttcaa taagtccagt atctaatcca ctagctgctg cttgtaaagc
    68641 gacaccagcg cctgtgatac ccccacctat gataagtaaa tccagagtac gatcttgcat
    68701 tctttgtaag gctaaacgtc gtgtttctcg tgaaaattcc atctctacac ccccttaatc
    68761 ctcttgagca aatacttgtg tcgctttgac agcttttctc caccctttat acagtttttc
    68821 ttttcgagac tcattcattg ttgcttggaa aagttgtcct gttgcgttca gttcttttag
    68881 ttcatccata ctttcccaat agccaactga aagtccggct aagaaagctg cacccaaggc
    68941 agtagtttca aggttcttcg cacgagcaat atcaatccct aaaatatccg cttggaattg
    69001 cattaataaa ttattcattg cagctccacc atctacacga agttgttgga tatcaattcc
    69061 tgaatcaact tgcatagtgt caataacgtc tcgtacttga taagcaattg actgaagtgt
    69121 agctttaaca aaatcttctt tacttgtccc acgagttaat ccaaatactg accctctagc
    69181 atttgaatcc caatacggtg cacccaaacc tgtaaaagct ggaaccacat aaacctcatc
    69241 gtcacttgtt gatgattgag caaggccttc tgactcactt gacgtttcaa tcattctaag
    69301 accatcacgt aaccattgaa tagcgcttcc tgcaatgaag attgatcctt caagtgcata
    69361 atgaacctta ccattaatgc cgtaaccaat cgttgttaat agattatttt gagacagttg
    69421 catctcttcg cccgtattca tgataatgaa ggaacctgta ccataagtgt tttttaccat
    69481 accaggttca aaggccaact gtccaaataa tgctgcctgt tggtcaccag ccataccaga
    69541 aattggcacc tctccaccgt aaaaatggaa tggagtagtt ttcccataaa cttcagaatt
    69601 agattttact tctggtaaca ttgctttagg aatatttagt aactctaaaa tttcatcatc
    69661 ccatttaagt tctttaatat tataaagcat cgtacgagca gcatttgaat agtctgtaac
    69721 atggacgaga ccatcagtta acttccaaac taaccaagta tcaattgttc caaaaagcaa
    69781 ctctcctttt tcagcacgtt cctgtgctcc aggaacgtgg tctaaaatcc aacgaacttt
    69841 tgttgctgag aagtaagcat caatgacaag accagttttt tcatgaatca tgttagtatg
    69901 accttcttgc ttcaattgat cagcaatcgg ggctgtttgt cgcgattgcc atacaatagc
    69961 attataaatt ggaagacctg tttttttatc ccaaacaaca gttgtttcac gttgattggt
    70021 aatcccaata gcctctatct gtccaggttt gatgcttgat tcaataaaag ctccagcaat
    70081 gacagattga acagaattcc aaatctgatt agcattatgc tctacccagc ctgcttgagg
    70141 aaaaatttgg ggaaattctt tttggctact cgcaattttt tcaccttttt tattaaaaat
    70201 aattgctcga gagcttgttg ttccctggtc aatcgccata atgtattttt cttctgaaga
    70261 cataaacttc ctccttgaaa gtaagcgttt tctttttgta attctattat acctaaatca
    70321 gtctatcctg cactatcaaa aatttaatat gctcctaaaa tttttgataa gatatcatca
    70381 ataacaccaa attagccaaa taatagctaa atcaataatc attccgatat accaaactag
    70441 tctgaaatac catttagtcg ttttatgacg aaaaacttct ccacctagga atgcaccaaa
    70501 tccccctaat aataaactct ctatcatcaa tgttttctca ctaatacgcc atgcccctct
    70561 aattgcacgc cttttatcaa tagcataggt tgcaaatact aaaatattcc ataacagtaa
    70621 tgctaatagt aattcaatca ttattttttc cttcctaata aaaaaggcga gacaaagatt
    70681 atcttttgcc ccacccaagt gttgacttta atctaaaatt gtctcaccat taagaatttg
    70741 gatcatccaa actacgacca tgtagtcctt tttctctctg gacttgacga agtttttccg
    70801 gggtaacatc attgccctca tcatccacta atttaattcc ttcaacgtgg tgacggactg
    70861 atcggcggaa accttcaata tactcttcac ggagctttgc ttgttcaacc ttctcttctc
    70921 cggtcaatcc aactgttttt ttcttttttg aaagctcgtt aatacgagca atttttttag
    70981 gatccataaa atgcctcctt attttgtgtt taataagtta aaagcagcga ctgttcttgc
    71041 ctttgcaaca aggctattga gtaaagccag acgattttgt ttcatttttt catcatctgt
    71101 cattaccatt gtattatcaa agaaatcatt aataattgga ctaagtgcga aaagcttatc
    71161 taagttgtca tgcatatcct cagtcagttc aagtgacaaa atagcttgat atagcgcctt
    71221 ctcttgatta ttttcaaaca atgaagagtc caccaataca gaatgagtaa ctttttcagc
    71281 taaattaaat gcacgtgaca aactttcaac ggaaacttta tattgagcat ccttgctctt
    71341 ttgcaccaat gcttgactag cttctagaat aaggctaaca atataagtgt ctgattctaa
    71401 aacggcttct cgaatatctt tagggacctt agagccaatc atcttttcaa tacgagctga
    71461 aataaaggcc atgacctctt tttgattagc ataatctaaa ctagcaaacg atagaccata
    71521 aaggttagtt actaattcat caagtgggat atcccaacca aaagcttcca aaatacggac
    71581 aatcccctga gtagctcgac gaagagcata tggatcattt gaaccacttg gaatcaaacc
    71641 aactgagaag aatgaaagta aggtgtcaaa tttatcagct aaagctagaa ttgcaccaac
    71701 tctggtttct ggaagctctc catctgcaga tgtcggcata taatgttcac gtatcgctgc
    71761 tgctactgcg ggctgttccc ctgcaagtaa ggcatatttc tcacccataa taccttgtaa
    71821 ttcatcaaac tcatcaacca ttccagttaa gaggtcaaat ttataaatac ttgcagctcg
    71881 aagcacagct aatttttctt catcagaaag atctgccttc tcagccaaat attgtgaaat
    71941 aacttttaca cgatccatat gttcataaag tgaaccaatt ttttcatgga aagttacttg
    72001 ttttaatttc tcaactaaat ctgcaatatt aagtttctga tcttcttgcc agaagaattc
    72061 accatcttca agacgagcca ctaaaacttt ttcattacct tttataacat tttcgatatg
    72121 ctcagcattt ccattacgca cagaaataaa gtttggtaac aattttccat ccctgtcgcg
    72181 aacaacaaaa tagcgttgat gatttttcat tgaagtgact aaaacttctt caggtacatc
    72241 aaggtatttc tcgtcaaaag aacccaagaa tgctgtcgga tactcaacta aattaagcac
    72301 ttcatttagt aaatcttcgt caatttcaac tgaaatattt ttcttttctt cgatagcatg
    72361 aatttgatta acaatcattt gctggcgttc ttttgcatca gcaataacaa attgttgtcg
    72421 caagtcatct tcatacgaac tggcagatga aatttcagtg tcagaaccca agaaacggtg
    72481 tcctcgacta atacgccctg aatgaatatc aaggaagtct agctctaagg cttgatcatc
    72541 taataatacc actaaagtgt gaactggacg aatatattca aaactattat ttgcccaatg
    72601 catgttcact gggaaagtca attctgttaa tacctctgtc acactcgcta aaacatcaat
    72661 cgcacttttt ccagtttcat gcttagtgac ataaacatat tcttctccct taacttcacg
    72721 aaattcgata tcatcaacac ttaaaccttt tccgcgaaca aaaccttggg ctgctttaga
    72781 aaaattccct tctgcatcca aagcaatttt cttagaagga cccttgaaat cttctgttaa
    72841 atctgtctgc tggtctgcca atcctttcac acgaacagcc aaacgacgtg gagttgaaaa
    72901 agtttgaaca gtttcaaatg ataaacgatg atcctctaaa aacttgacca ttttttggcc
    72961 caattgcttc tcactcggtg tcacaacata agctggcaac tcttcaaggc caagctccaa
    73021 taataaatct ttagtcattt cctactcctt actgtaaaac gcttcaaatg cttctcttaa
    73081 atcctgattc atcttatcaa gaacaacttg cctatctaaa atatcggtac cttctgcacg
    73141 aacaacacag aaatcatcaa aagccataaa atctttaaag atagctttta aataatgaac
    73201 aggaaattca tatggactat actgtccttt agaataaata gaacccgcag attctaacat
    73261 aagcagacga tagtcatctg tcattaatcc gactgaacca ttttcagtgt acttgaaagt
    73321 ctcacctgca ataaaaatat tatcaatata agtttttgct ctagcagtca cattgtaatt
    73381 gtgcattgga gaaacaaaga caatacggtg atgtgactta aactgttcaa gtaaatcttt
    73441 agagatttta gcttgaacaa tctcatcggc tgttaattcc aaaccctttc tttgtttaga
    73501 ccaaatcgaa agtagtactt ctttcgtaat ttctggcaaa acacaatcat ataaatttaa
    73561 aatagataac tgatggttag gaaaatgctc attataaagt tcaataaact tttcttgtag
    73621 aatagttgta aatgagtacg gattcgaaaa atcaggatga ctataaacaa tgagtgtgtt
    73681 cattagtctt cctcagcaag taatttgata cgagtttcct catccaaaag tgggaaacca
    73741 agttttttac gttcggcaac aaaggtttta gcaacaacac gtgctaaatt ccggatacgt
    73801 gcaatatagc ctgcacgctc agtaacagat actgctccac gtgcatctaa taaattaaag
    73861 gtgtgtgaac atttaaggac gtagtcataa gctggatgaa caagtccttc ttctaaagca
    73921 cgttttgctt ctctttcaaa tttctcaaag ttttcaagta acattacttg gtctgatatc
    73981 tcaaatgaat attttgaatg ctcatactct ggctgagtaa agatttcacc atatttaaca
    74041 cctggtgccc actcaatatc ataaacagaa tctacctctt gaatataaga ggctaaacgc
    74101 tctagtccat aagtaacttc agaagttaca ggacctgttt gaagtccacc tacttgttgg
    74161 aaataagtaa attgtgttat ttccattcca tcaagccaaa cttcccaacc taatccagct
    74221 gaacctgtag atggattttc ccagttatct tcaacaaaac ggatatcatg ctcaagtgga
    74281 ttgataccta gtaattccaa agacttcaaa taaagttctt gaatatttga cggcgaaggc
    74341 ttcattacca cttgaaattg atgatgttgg taaagacggt ttggattttc gccatagcgg
    74401 ccgtcagctg gacgacgtga tggttcaacg tatgcagcat tccaaggctc agggcctata
    74461 gcacgtaaga aagtataagg gctcattgtc cctgctcctt tctcattatc ataagcttgc
    74521 attaacatac agccttggtc attccaaaac tgttgtaaag ttagaataat ttcttgaaaa
    74581 gttaatttct tagacatcat tttccttctt tcacacttta ttacagctgt tgggaaggtg
    74641 acaacaattg acttaaaacg gacaaatgat aaaaaccacg tcctacacca cctatgccca
    74701 acagcacagg ggcgtcaaag acgcggttcc accctaattt attacttcat ttttgattgt
    74761 acttttactc actaaaaagt gccaatattc atcctattct acccaacttt cactaccttg
    74821 agctcgctaa aaaaacagct gatgaacacc tcttcttact gagacagtat aacaaaaaat
    74881 gacttttatg tctactattt atcttattct taatataccc tagacttttt gattccattt
    74941 ggaataatga tcgtttttaa taataagttt tcttcacgaa ttcttaaata aataacaaaa
    75001 gcataaattg gcaagacaaa taatgctgtg tatttagcat gacacaataa agttacacct
    75061 aaaagttcag gagcaatatt taagaaatag ttaggatgct taacaaccct aaataaccag
    75121 tgatctacaa attgatgatt tttatctacc attaatttca ctgtccaaat acgaccaagt
    75181 aaacgtgtta ctgtatgtaa cataaacaca gaaaaaatca tcaagctaag cccgattacg
    75241 ctaacaaaat taaaacttgc ttttgttaga atagcctcag ttacagatga aaaataaatg
    75301 ataatatgaa gaactgtgat tgcttttgaa acacctacac catattcttt agcaccattt
    75361 tttcttaagg ccttttcatt agctatagat agctttaaat aagctaaacg aataataaac
    75421 atagcggcca ttaacccaat aataattgac attgt
//
LOCUS       DATRNK010000003        68195 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.003, whole genome shotgun sequence.
ACCESSION   DATRNK010000003 DATRNK010000000
VERSION     DATRNK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 68195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68195
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.003"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            155..946
                     /locus_tag="VBC52_000181"
                     /pseudo
     CDS             155..946
                     /locus_tag="VBC52_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743858.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1013..1558
                     /locus_tag="VBC52_000182"
     CDS             1013..1558
                     /locus_tag="VBC52_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881790.1"
                     /translation="MKKITTLILASSLLLVATTSVKADDNFEMPTRYVKMSEKSKAFY
                     QRLQEKQRKAHTTVKTFNNSEIRHQLPLKQEKARNDIYNLGILISQESKGFIQRIDNA
                     YSLENVSDIVNEAQALYKRNYDLFEKIKSTRDKVQVLLASHQDNTDLKNFYAELDDMY
                     EHVYLNESRVEAINRNIQKYN"
     regulatory      1709..1798
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1954..2520
                     /gene="thiW"
                     /locus_tag="VBC52_000183"
     CDS             1954..2520
                     /gene="thiW"
                     /locus_tag="VBC52_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEO6881791.1"
                     /translation="MTNHKTKKLTVLAIMIALDVFLTPIFRIEGMAFMSSVVNIIAGV
                     IMGPIYALVMALVTAFIRILTQAGVASVAPLAILGAGPGAFLAGWFYRFYAKDWMSWV
                     GEFIGTGIIGALLSAPIMVWFWTWTADGNHEVLAKASAAQSIFLFMPRFIGATIIGGV
                     IALLLLQGLKRVPVFMTIQALFKGEKVK"
     gene            2517..3071
                     /locus_tag="VBC52_000184"
     CDS             2517..3071
                     /locus_tag="VBC52_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6881792.1"
                     /translation="MKLSLKDVLLIALLAVVLGVVYFGAGYISNAFVPFVGPIAHEVI
                     YGIWFVAGPMALYILRKPGTAIVAELLAALIEVLIGSIYGPSVLVIGTLQGLGSELGF
                     TLFRYHNYKLPAFILSAILTSIFSFAWSFYANGLSAFSFSYNILMLIVRTVSSIIFFL
                     LTKNICDQLHRSGVLNAYGIHERK"
     gene            3075..4361
                     /locus_tag="VBC52_000185"
     CDS             3075..4361
                     /locus_tag="VBC52_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6881793.1"
                     /translation="MLSVEKLACTHGDSHYLFDEVTFSLNPGERILISGYSGCGKSTL
                     ALLLSGLKESGKGQVLLNGSLIEPSDVGFLFQNPDLQFCMDTVAHELYFILENLQIEP
                     EQMQDRSEFVLAQVGLKGFQNRLIYTLSQGEKQRLALATIFLKSPKLIILDEAFANLD
                     QESASQLLQLVLNYQANNQSMLIVIDHLITYYQDIMDHYFWLEKRLTRVNFDYMLNRL
                     NVFELEKKSHNTGDKLLSIKDFQVKLSKNKFISYLDFDLASGERLCLDGPSGVGKSSL
                     FMGLLGLYRTKGKKQFTHRKQIPISFLFQNPLDQFIFSTVYDEIFQVCKDSNKARDIL
                     ETINLWDKKQFSPFQLSQGQQRRLAIGSILASDSKLLLLDEPTYGQDAYHANMITTLL
                     LSYCHKNHCGVIFTSHDPHLKECFATRFLEVSSCSI"
     gene            4349..5017
                     /locus_tag="VBC52_000186"
     CDS             4349..5017
                     /locus_tag="VBC52_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEO6881794.1"
                     /translation="MFNLKNYHPAITFLCLLIPTFSFSFTLRLRTSLLFLIIVVTLQC
                     FVKVSLKTWAKVNLISFVMGLSLFLGTYFWGKLPHQFVLASLVACRPLIFMNVGLLFH
                     ASHSNYDFIESLYQTFKVPSHFAYGIFAVFNLLPLIKLQYQRNRLAFRLKNQVTWALS
                     PRLILSVLLKTIYWVEQLELAMLSKGFEVGKERTHASTYPVRFRDYSLLGMSILLSIG
                     MIFK"
     gene            5101..5781
                     /gene="tenA"
                     /locus_tag="VBC52_000187"
     CDS             5101..5781
                     /gene="tenA"
                     /locus_tag="VBC52_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEO6881795.1"
                     /translation="MLKDKMIAKGAKYIQSIYQDPFIQGIIKGRLDHDVICHYLQADN
                     IYLGKFADIYALCLAKSDNLRDKQFFLEQIDFTLNRELADGEGPHQALAAYTNRSYQD
                     IIEKGVWYPSADHYIKHMYFHFYENGIAGALAAMSPCPWIYHQLAKKIIEENQFLNGN
                     PFNNWITFYANDTVEELMENYFRMMDYYAQNLSKEKQADLVDAFVKSCQHERRFFQMA
                     INQEKWED"
     gene            5783..6580
                     /gene="thiD"
                     /locus_tag="VBC52_000188"
     CDS             5783..6580
                     /gene="thiD"
                     /locus_tag="VBC52_000188"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO6881796.1"
                     /translation="MTYLAPVLTIAGTDPSGGAGIMADLKTFQARRTYGMAVVTSVVA
                     QNTCGVRGVQHIETAIIDQQLACVYDDIKPKAVKTGMLAERETISLVASYLKKYPQPY
                     VLDPVMVATSGHRLIDSDAVEALKEDLLPLATIITPNLPEAEVLVGYDLSDEVSIIKA
                     GYDIQKQYSVRNVLIKGGHLDGLAKDYLFLEKEGLITLSNQRINTIHTHGTGCTFAAV
                     VAAELAKGQSILNAVSTAKSFITSAIETAPELGLGNGPVNHTSYQGD"
     gene            6582..7352
                     /gene="thiM"
                     /locus_tag="VBC52_000189"
     CDS             6582..7352
                     /gene="thiM"
                     /locus_tag="VBC52_000189"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEO6881797.1"
                     /translation="MYLEQLKEVNPLTICITNNVVKNFTANGLLALGASPAMSECIED
                     LEDLLKVADALLINIGTLTKESWQLYQEAIKIANKNQVPVVLDPVAAGASRFRLEVSL
                     DLLKNYSISLLRGNGSEIAALIGEKQASKGADGGKVADLESIAVKANQVFDVPVVVTG
                     ETDAIAVRGEVRLLQNGSPLMPLVTGTGCLLGAVLAAFIGSSDRSDDLACLTEAMTVY
                     NVAGEIAEKVAKGKGVGSFQVAFLDALSQMKSEMIMDK"
     gene            7366..8037
                     /locus_tag="VBC52_000190"
     CDS             7366..8037
                     /locus_tag="VBC52_000190"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001904425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEO6881798.1"
                     /translation="MKDTLKLYFVCGTVDCSRKNILTVVEEALQAGITLFQFREKGFT
                     ALQGKEKIAMAKQLQILCKQYQVPFIIDDDIDLVELIDADGLHIGQNDLPVDEARRRL
                     PDKIIGLSVSTMDEYQKSQLSVVDYIGIGPFNPTQSKADAKPAVGNRTTKAVREINQD
                     IPIVAIGGITSDFVHDIIESGADGIAVISAISKANHIVDATRQLRYEVEKALVNRQKH
                     SDVIK"
     gene            8164..9423
                     /locus_tag="VBC52_000191"
     CDS             8164..9423
                     /locus_tag="VBC52_000191"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO6881799.1"
                     /translation="MRKIIINGGKQLTGEVAVSGAKNSVVALIPATILADDVVVLDGV
                     PAISDVDSLVDIMETMGAKIKRYGETLEIDPCGVKDIPMPYGKINSLRASYYFYGSLL
                     GRYGQATLGLPGGCDLGPRPIDLHLKAFEAMGASVSYEGDSMRLATNGKPLQGANIYM
                     DTVSVGATINTIIAAAKANGRTVIENAAREPEIIDVATLLNNMGAHIRGAGTDVITIE
                     GVKSLHGTRHQVIPDRIEAGTYIAMAAAIGRGIKVTNVLYEHLESFIAKLDEMGVRMT
                     VEEDSIFVEEQERLKAVSIKTSPYPGFATDLQQPLTPLLLTAEGNGSLLDTIYEKRVN
                     HVPELARMGANISTLGGKIVYSGPNQLSGAPVKATDLRAGAALVIAGLMAEGRTEITN
                     IEFILRGYSNIIEKLTSLGADIQLVEE"
     gene            9525..10079
                     /locus_tag="VBC52_000192"
     CDS             9525..10079
                     /locus_tag="VBC52_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6881800.1"
                     /translation="MDIWTNLGRFAFIETEHVNLRPVAYTDREAFWRIASKRTNLQFI
                     FPVQTSKKESDFLLVHSFMKEPLGVWAIEDKVSHKMFGVIRFENIDLSKKTAEIGYFL
                     KESSWGQGIMTECLKTLSFFAFREFGMDKLIIVTHKENIASQKVALKAHFKQSRSFKG
                     SDRYTRRIRDYIEFQLTRGDYRYE"
     gene            10072..11355
                     /locus_tag="VBC52_000193"
     CDS             10072..11355
                     /locus_tag="VBC52_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6881801.1"
                     /translation="MSKHQEILEYLENLAVGKRVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETRPRSGTVRVAQKAKVNIEKLTYAEIARISDSQVVAGIEGLSKEFSKFSIGA
                     MTHRNIEKYLVQGGLLIVGDRDEIQHLALQHQNAILVTGGFNVSPSVCRLADKLQIPV
                     MVTHYDTFTVSTMINHTLSNAKIRTDLKTVEQVYQSQMDYGFLAQDDTVKEFNLLVKQ
                     TKNVRFPIVNQANVVVGVVSVQDILGKDKEVKLATVMSKNIIVAKPRMSLANISQKMI
                     FEDLNMMPVVSDDFELLGVITRRQAVENLSMSQGTDLYTYSDQILSNLQIEDGHFSFL
                     VEPAMIDHTGSLTQGVLTEFLKEICIRVLTRKHQRSIVVKQMTLYFLQPVQIDEIIMV
                     TPTIISEKRREATLDLELKLENKIIAKAMIAVKIN"
     gene            11378..12238
                     /locus_tag="VBC52_000194"
     CDS             11378..12238
                     /locus_tag="VBC52_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEO6881802.1"
                     /translation="MITLKSAREIEAMDRAGDFLASIHIGLRDIIKPGVDMWEVEEYV
                     RRRCKEENVLPLQIGVDGAVMDYPYATCCGLNDEVAHAFPRHYTLKQGDLLKVDMVLS
                     EPLDKSIVDVSSLNFDNVAQMKKYTETYSGGLADSCWAYAVGEVSQEVKDLMSVTREA
                     MYIGIEKAVIGNRIGDIGAAIQDYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLREGMVLTIEPMINTGTWEIDTDMKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            12240..13160
                     /locus_tag="VBC52_000195"
     CDS             12240..13160
                     /locus_tag="VBC52_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEO6881803.1"
                     /translation="MKLKKFFEDLLAKLEYRPIQVFMRHFQSAEMDLSAIAVAYYLLV
                     TAFPLLVIAANIFPYFHINVSDLLSFMQKNLPKNIYEPASRLAVDAFSKPSTGILGFA
                     SLTAFWTMSKSLTSLQKAINKAYGVDQHRDFVISRLVGVGTGLIILFLLTFVLIFSTF
                     SKPVLQIIVNMYDLGDTLTAWLLNLAQPVTFLTIFLGIGILYFILPNARIRKVRYVIP
                     GTLFSTFVIGFFSNLISQYVLNRVEKMVDIKTFGSVVIFILMLWFIFLAHIMILGAIL
                     NASVQEIATGKIESRRGDIMSLIQKSKEEK"
     gene            complement(13177..13632)
                     /locus_tag="VBC52_000196"
     CDS             complement(13177..13632)
                     /locus_tag="VBC52_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEO6881804.1"
                     /translation="MVKLKSLLKKSIQNEVSLYLLFGLLTSLLYLVIRQGIFNFSQDA
                     PFSAIVANIIAILFAFFTNDRFVFKQTKIGQLQRLQTFVIARLGTLGLDLILAVIFVD
                     QFPSIIGQFVQHNLNKINTIESLVSQILIILLNYILSKFVIFKDKKRQL"
     regulatory      13703..13816
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            13814..14323
                     /locus_tag="VBC52_000197"
     CDS             13814..14323
                     /locus_tag="VBC52_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEO6881805.1"
                     /translation="MNTFTTRDYAHMAIVTAIYIVLTITPPFNAIAYGAYQFRVSEML
                     NFLAFYHRKYLFAVTLGCMISNLYSFGMIDVFVGGGSTLLFVYLGTILFKQYQKDYLF
                     NGLINKAFFFFSFFFAASMITVAVELKIVAGLPLLLTWLTTAVGELASLLVGAVLVDK
                     LSRHVDFTK"
     gene            14463..16421
                     /gene="ligA"
                     /locus_tag="VBC52_000198"
     CDS             14463..16421
                     /gene="ligA"
                     /locus_tag="VBC52_000198"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEO6881806.1"
                     /translation="MENRMNELVSLLNQYAKEYYTQDNPTVSDSQYDQLYRELVELEK
                     QHPENILPNSPTHRVGGLVLEGFEKYQHEYPLYSLQDAFSKEELIAFDKRVKAEFPTA
                     AYMAELKIDGLSVSLTYVNGVLQVGATRGDGNIGENITENLKRVHDIPLHLDQSLDIT
                     VRGECYLPKESFEAINIEKRANGEQEFANPRNAAAGTLRQLNTGIVAKRKLATFLYQE
                     ASPTQKETQDDVLKELESYGFSVNHHRLISSSMEKIWDFIQTIEKDRVSLPYDIDGIV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVVVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEGEVALRCINPLCPNQIKERLAHFASRDAMNITGFGPSLVEKLFD
                     AHLIADVADIYRLSIENLLTLDGIKEKSATKIYHAIQSSKENSAEKLLFGLGIRHVGS
                     KASRLLLEEFGNLRQLSQASQESIASIDGLGGVIAKSLHTFFEKEEVDKLLEELTSYN
                     VNFNYLGKRVSTDAQLSGLTVVLTGKLEKMTRNEAKEKLQNLGAKVTGSVSKKTDLIV
                     AGSDAGSKLTKAQDLGITIQDEDWLLNL"
     gene            16433..17452
                     /locus_tag="VBC52_000199"
     CDS             16433..17452
                     /locus_tag="VBC52_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO6881807.1"
                     /translation="MKKQKKARLIYNPTSGQEIMKKNVAEVLDILEGFGYETSAFQTT
                     PTKNSARDEATRAAQAGFDLIVAAGGDGTINEVVNGIAPLKRRPKMAIIPTGTTNDFA
                     RALKIPRGNPIEATKLIGKNQIVKMDIGQAQEDNYFINIAAAGSLTELTYSVPSQLKT
                     TFGYLAYLAKGVELLPRVRKVPVKITHDKGEFIGDASMIFVAITNSVGGFEQIAPDAK
                     LDDGKFTLILVKTANLIEIMHLIRLVLAGGKHINDKRVEYIKTSYLTIEPLSDERMMI
                     NLDGEYGGDAPITLANLKNHIRFFANTDEISDDALVLDKDELAIEAIAQKFANEVDDL
                     EGLGD"
     gene            17456..19756
                     /gene="pulA"
                     /locus_tag="VBC52_000200"
     CDS             17456..19756
                     /gene="pulA"
                     /locus_tag="VBC52_000200"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEO6881808.1"
                     /translation="MDNTVIVHYHSRKKNYFNLSLWQWRDGSEGQDAHFSRFDSFGAV
                     AILKYPLPYFLSHYYVIVKDKNWHHKTIDYRIDCTQGGARKEVWIVDGDDNLYYSRQA
                     AVSSHHYSRRQPHAYDMAINSRAFDKKWGFDGWLGFEYSETKTAFRLWAPTAERVELI
                     LYHSTDETASVSKVLSMKRGTAVNYKNHKENTHGVWFTELEGNYNYQAYTYRVYYRRR
                     TFKITRDPYSIATTANGKRSIVIAPEALTPEGFKISHGKEAKWRLENPNQAVIYEMHV
                     RDFSISETSGVKTDYHGKFKGLHQKGTVNQHGDKTTFDYVQDLGVNYIQLQPIFDHHQ
                     TFDDDGHYAYNWGYDPENYNVPEASFSSNPHEPATRILELKSAIQAYHDAGIGVIMDV
                     VYNHTFSSTDSAFQLTVPDYYYRMNHNGTFQNGSGCGNETASEKEMCRKYILDSVLYW
                     VKEYNIDGFRFDLMGLHDVETMNIIRNELNKIDPRILVYGEGWDMGAGLTPQNKAKKD
                     NAYQMPGIGFFNDDVRDAVKGAEIYGEFKKGLVSGNSTEDIVAKGILGSDELVSYIDP
                     SQVLNYVEAHDNYNLNDLLWELHPNDNEKQHIYRVEVASAMNLLMQGMAFMQLGQEFL
                     RTKCYPTGDKGQLTQADKERAMNSYNAPDQVNQVNWDNVTFHKSTINFIRKIITLKTN
                     SPYFSYSSFEEIRKHVFVESAQYHSGFISFTVEEHKKIKIIFNIKGKRLQNSFIGDII
                     ISNHEEGKAIDSLDDELSVMILDITK"
     gene            19962..21830
                     /gene="glgB"
                     /locus_tag="VBC52_000201"
     CDS             19962..21830
                     /gene="glgB"
                     /locus_tag="VBC52_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEO6881809.1"
                     /translation="MSNKDELYTFGIGENFHLQNYLGVHSENGSFCFRVWAPNAENVQ
                     VIGDFTDWRNRPLQMNKNQAGVWEANSLDAREGDLYKYLVTRKGGQVVEKIDPMAVYM
                     ERRPGTASVIKVLRNKKWEDGLWMGRRKRLGFQKRPINIYEVHAGSWKKDDFGHPMTF
                     SQLKDYLIPYLVEMNYTHVEFMPLMAHPLDMSWGYQLMGYFAFEHTYGTPEEFQDFVE
                     ACHKNNIGVLVDWVPGHFIQNDDALAYFDGTATYEYQNHDRAHNYRWGALNFDLGKNQ
                     VQSFLISSALFWIEHYHIDGIRVDAVSNMLYLDYDEGPWEANQFGDNRNLEGYHFLRK
                     LNKVIKERHPNVMMIAEESTASTPITKDLESGGLGFDFKWNMGWMNDILRFYEEDPLY
                     RQYDFNLVTFSFMYIFNENFVLAFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNLYAYQ
                     MCHPGKKLLFMGSEFGQFLEWKYNDQLEWENLNDDMNQKMQRYTKQLNQFYKDHKCLW
                     RIDDSFDGLEIIDADNKSETVLSFIRKDDKGDLLLCVFNMTPVERPNFTIGVPQAGIY
                     EEVLNTEMEEFGGVWKNHNPVTKTQVATWKDYDHTLSFTLPALGASVWRIKRRLRK"
     gene            21872..23011
                     /locus_tag="VBC52_000202"
     CDS             21872..23011
                     /locus_tag="VBC52_000202"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019785022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEO6881810.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGINNVGVITQYQPLELNTHIGNGSSWGLDGIDSGVTVLQPYSATEGNRWFQGT
                     SHAIYQNIDYIDRINPEYVLILSGDHIYKMNYDDMLQTHKDNLASLTVAVLDVPLKEA
                     SRFGIMNTDSNDRIVEFEEKPEHPKSTKASMGIYIFDWKRLRTVLIDGEKNGIDMSDF
                     GKNVIPAYLESGERVYTYNFDGYWKDVGTIESLWEANMEYIGEDNKLHSRDRSWKIYS
                     KNLIAPPNFMTEDANVKDSLVVDGCFVAGNVEHSILSTNVQVKPNAIIKDSFVMSGAT
                     IGEGAKINRAIIGEDAVIGDGVVIDGSKEVEVIGYKEVAGVPNED"
     gene            23001..24134
                     /gene="glgD"
                     /locus_tag="VBC52_000203"
     CDS             23001..24134
                     /gene="glgD"
                     /locus_tag="VBC52_000203"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEO6881811.1"
                     /translation="MKIDKYTAILGNTVSYPDMHSLTDNRPLASLPFDGKYRLIDFQL
                     SSLANAGVRSIYGIFRGNNVRSVFDHIRSGREWGLGTLLSHYSLGFYDTDDDTRTVNG
                     DYYDQILTYLRRSGSDQTIYMSCDMLCNIDLSQVIHLHNANGAQATVVYKKMPRSAIA
                     EANQVLTIDESDHVVSLGKAVDNDDNQKMSAEIYVIDTPWLIEKMEEEAQNNEPRKLR
                     FLLRDLIVESNALAFEYTGYLSNISSIKSYYDANMDMLTPNKFYSLFFSNQKVYTKVK
                     NEEATYFDKQSNVSNSQLASGSIIKGYLDHSIVSRNCLLEKGTRVVNSIIFPKVKIGE
                     GATIENTIIDKCVKVASGVTLKGSLDKPLVIPKFSEINEDIIQ"
     gene            24131..25561
                     /gene="glgA"
                     /locus_tag="VBC52_000204"
     CDS             24131..25561
                     /gene="glgA"
                     /locus_tag="VBC52_000204"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEO6881812.1"
                     /translation="MKIMFVAAEGAPFAKTGGLGDVIGALPKSLSKKGHDVAVVMPYY
                     DMVDQKFGDQIENLMYFYTDVGWRHQYVGVKRLSQDNVTFYFIDNQYYFYRGHVYGDW
                     DDGERFAYFQLAALELMEKIDFIPDVLHVHDYHTAMIPFLLKEKYHWIQAYNNIRAVF
                     TIHNIEFQGQFGPEMLGDLFGVGAERYEDGTLRWNNCLNWMKAAILYSDRVTTVSPSY
                     ANEIKTPEFGKGLDQIMRMEAGKLSGIVNGIDSDLLNPETDAFLPYHFSKSNLEGKIK
                     NKLALQENLGLPQDKNVPLIGIVSRLTDQKGFDIIASELDNMLQQDIQMVILGTGYHH
                     FEETFSYFASRYPEKLSANITFDLRLAQQIYAASDIFMMPSAFEPCGLSQMMAMRYGS
                     LPLVHEVGGLKDTVVAFNQFDGSGTGFSFNHFSGYWLMQTLKLALEVYNDYPEAWKKL
                     QWQAMSKDFSWDTACVAYEQLYQQLQ"
     gene            25893..26093
                     /locus_tag="VBC52_000205"
     CDS             25893..26093
                     /locus_tag="VBC52_000205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEO6881813.1"
                     /translation="MNLAILALGFAVMGVSIGEGILVANIAKSAARQPEMFSKLQTLM
                     FTGVAFIEGTFFVLFAFTFLVR"
     gene            26126..26842
                     /gene="atpB"
                     /locus_tag="VBC52_000206"
     CDS             26126..26842
                     /gene="atpB"
                     /locus_tag="VBC52_000206"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEO6881814.1"
                     /translation="MESTSNPTVSFLGIDFDLTILAMSLLTITIIFILVFWASRKMTI
                     KPKGKQNVLEYVYELVNNTISQNLGHYTKNYSLLMFILFSFVFIANNLGLMTSLKTHE
                     HNFWTSPTANFGVDITLSLLVAFICHIEGIRKKGIGGYLKGFLSPTPAMLPMNLLEEV
                     TNVASLALRLFGNIFSGEVVTGLLLQLAVLSPFTGPLAFALNIVWTAFSMFIGFIQAY
                     VFIILSSSYIGHKVHGDEEE"
     gene            26860..27357
                     /gene="atpF"
                     /locus_tag="VBC52_000207"
     CDS             26860..27357
                     /gene="atpF"
                     /locus_tag="VBC52_000207"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEO6881815.1"
                     /translation="MSILINSTTIGDIIIVSGSVLLLFILIKTFAWKQITGIFEAREQ
                     KIANDIDTAEQARQQAEAFATKREEELSNAKTEANQIIDNAKETGLAKGDQIISEAKT
                     EADRLKEKAHQDIAQNKAEALADVKGEVADLTVLLAEKIMVSNLDKEAQSNLIDSYIK
                     KLGDA"
     gene            27357..27893
                     /locus_tag="VBC52_000208"
     CDS             27357..27893
                     /locus_tag="VBC52_000208"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEO6881816.1"
                     /translation="MNKKTQALIEQYSKSLVEVAIEHKIVEKIQQEVAALIDIFETSE
                     LEGVLSSLAVSHDEKQHFVKTLQTSCSTYLVNFLEVIVQNEREALLYPILKSVDQELI
                     KVNGQYPIQITTAVALSPEQKERLFDIAKTKLALPNGQLVEHIDPSIVGGFVVNANNK
                     VIDASVRNQLHQFKMKLK"
     gene            27909..29414
                     /locus_tag="VBC52_000209"
     CDS             27909..29414
                     /locus_tag="VBC52_000209"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEO6881817.1"
                     /translation="MAINAQEISALIKKQIEDFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFSNGAYGMAQNLESNDVGIIILGDFSEIREGDVVKRTGKIMEVPVGEA
                     MIGRVVNPLGQPVDGLGEIETTATRPVETPAPGVMQRKSVFEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRKYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYNGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDALGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGAAQIKAMKRVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEVLKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     NDILAFEEALYDYFDAHYDNLFETIRTTKDLPEEAELDAAIQAFKDQSQFK"
     gene            29430..30311
                     /locus_tag="VBC52_000210"
     CDS             29430..30311
                     /locus_tag="VBC52_000210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEO6881818.1"
                     /translation="MAGSLSEIKDKILSTEKTSKITSAMQMVSSAKLVKSEQAARDFQ
                     VYASKIRQITTNLLKSDLVSGSDNPMLSSRPVKKTGYIVITSDKGLVGGYNSKILKAM
                     MDTITDYHTENDDYAIISIGSVGSDFFKARGMNVSFELRGLEDQPSFDQVGKIIAQAV
                     EMYKNELFDELYVCYNHHVNSLTSQVRMQQMLPIKELDAEEASEDRVITGFELEPNRE
                     VILEQLLPQYTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQA
                     AITQEITEIVAGANALE"
     gene            30385..31791
                     /gene="atpD"
                     /locus_tag="VBC52_000211"
     CDS             30385..31791
                     /gene="atpD"
                     /locus_tag="VBC52_000211"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEO6881819.1"
                     /translation="MSSGKIAQVVGPVVDVVFASGDKLPEINNALIVYKNGDKSQKVV
                     LEVALELGDGLVRTIAMESTDGLTRGLEVLDTGRAISVPVGKDTLGRVFNVLGDAIDL
                     EEPFAEDAERQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALTPEIVGDEHYEVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAETFTGQPGSYVPVEETVRGFKEILDGKHDQIPEDAF
                     RMVGGIEDVIAKAEKMNY"
     gene            31804..32217
                     /locus_tag="VBC52_000212"
     CDS             31804..32217
                     /locus_tag="VBC52_000212"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEO6881820.1"
                     /translation="MAQLTVQVVTPDGIRYDHHASLITVRTPDGEMGILPGHINLIAP
                     LNVHQMKINRSHQEGVDWVAVNGGIIEVNEDQVTIVADSAERARDIDLNRAERAKERA
                     ERALEKAQTTQNIDEMRRAEVALRRAINRISVGKK"
     gene            32282..32512
                     /locus_tag="VBC52_000213"
     CDS             32282..32512
                     /locus_tag="VBC52_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEO6881821.1"
                     /translation="MILNIVTIVSNFIFIALFYQLFVDLFDWSKMIKMSPQNISKLKV
                     FILLISIVGGYVVSHFLLEVIQLCQSIFWALQ"
     gene            32575..33846
                     /gene="murA"
                     /locus_tag="VBC52_000214"
     CDS             32575..33846
                     /gene="murA"
                     /locus_tag="VBC52_000214"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO6881822.1"
                     /translation="MDKIIVEGGQTQLQGQVVIEGAKNAVLPLLAATILPSQGKTLLT
                     NVPILSDVFTMNNVVRGLDIQVDFNCDKKEILVDASGDILDVAPYEFVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPIDLHLKGLEAMGATITQNGGDITAQAEKLKGANI
                     YMDFPSVGATQNLMMAATLASGTTTIENAAREPEIVDLAQLLNKMGAKVKGAGTETLT
                     IIGVDALHGTEHDVVQDRIEAGTFMVAAAMTSGNVLVKDAIWEHNRPLISKLMEMGVE
                     VSEEEDGIRVKADTKKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMIETVFEN
                     RFQHLEEMRRMGLQTEILRDTAMIHGGRALQGAPVMSTDLRASAALILAGMVAQGQTV
                     VGQLTHLDRGYYQFHEKLAALGANIKRVSEA"
     gene            33848..34039
                     /locus_tag="VBC52_000215"
     CDS             33848..34039
                     /locus_tag="VBC52_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO6881823.1"
                     /translation="MASSGWKYVLKQIGLIVLVILLALLFLAVGLMLGYSVFGDGEHA
                     YSILSLDKWQNIIGKFLGK"
     gene            34114..34971
                     /locus_tag="VBC52_000216"
     CDS             34114..34971
                     /locus_tag="VBC52_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6881824.1"
                     /translation="MTKKQKQITASIVTLLFSLLAIGLGESKSLPNDHILKQVTDLII
                     SNESGKNTSLKNVSGTPSRELSESVLTSNVKKQLGTNIAWNQSGAFIINQNKTDLNAK
                     VSSAPYAINEIKKVNNQIVPTKANALLTKATRQYRNREETGNGRTYWKPAGWHQINGL
                     KGSYNHAVDRGHLIGYALVGSLRGFDASTSNPKNIATQAAWANQANSNQSTGQNYYET
                     LVRKALDRHKTVRYRVTLIYDRDNLLSSGSHIEAKSSDGSLEFNVFIPNVQSGLLFDY
                     ATGKVKQTK"
     gene            35263..36303
                     /gene="pheS"
                     /locus_tag="VBC52_000217"
     CDS             35263..36303
                     /gene="pheS"
                     /locus_tag="VBC52_000217"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEO6881825.1"
                     /translation="MDLQKQLEELKISTQEKLKEMTGNHTKELQDLRVQVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDILTKAFEEQAKVVEAAKIQAQLESESVDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQVVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTMDQHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQI
                     EGLVVGENISMGDLKGTLQLISQKMFGAERKIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKQTGWIEILGAGMVHPSVLEMSGIDSEKYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDVRFTDQF"
     gene            36386..36907
                     /locus_tag="VBC52_000218"
     CDS             36386..36907
                     /locus_tag="VBC52_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6881826.1"
                     /translation="MSQITFKKVGLDNVNILQNIAIETFRQTFSHDNSEEQLQAFFNE
                     SYTLPVLKSEITHAESDTYFVYLDTDLVGYLKVNWGSQQTEKDLDKAFEIQRIYLLDA
                     YQGQGIGKATFEFALDLAYKSGLDWAWLGVWEFNHKAQAFYAKYGFEKFSEHQFSVGD
                     KVDTDWLLRKSLH"
     gene            36961..39366
                     /locus_tag="VBC52_000219"
     CDS             36961..39366
                     /locus_tag="VBC52_000219"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEO6881827.1"
                     /translation="MLVSYKWLKELVDVDVTTAELAEKMSTTGIEVEGVETPAEGLSK
                     LVVGHIVSCEDVPDTHLHLCQVDTGDDELRQVVCGAPNVKTGINVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSESIIPKEFSDGIQILPEGAIPGDSIFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYGKKVHFEEKNLIEEAERAADKISVVIESDK
                     VLSYSARIVKNVTVAPSPQWLQNKLMNAGIRPINNVVDVTNYVLLTYGQPMHAFDFDK
                     FDGTTIVARNAENGEKLITLDGEERDLIADDLVIAVNDQPVALAGVMGGQSTEIGSSS
                     KTVVLEAAVFNGTSIRKTSGRLNLRSESSSRFEKGINYDTVSEAMDFAAAMLQELAGG
                     QVLSGQVTEGVLPTEPVEVSTTLGYVNTRLGTELTYTDIEEVFEKLGFAISGSEVKFT
                     VLVPRRRWDIAIQADLVEEIARIYGYEKLPTTLPEAGATAGELTSMQRLRRRVRTVAE
                     GAGLSEIITYALTTPEKAVQFSTQATNITELMWPMTVDRSALRQNVVSGMLDTIAYNV
                     ARKNSNLAVYEIGKVFEQTGNPKEDLPTEVETFTFALTGLVEEKDFQTKAKPVDFFYA
                     KGIVEALFIKLKLDVTFVAQKGLASMHPGRTATILLDGKEIGFVGQVHPQTAKQYDIP
                     ETYVAEINLSTIESQMNQALIFEDITKYPSVSRDIALLLAESVSHHDIVSAIETSGVK
                     RLTAIKLFDVYAGNNIAEGYKSMAYSLTFQNPNDNLTDEEVAKYMEKITKSLVEKVNA
                     EIR"
     gene            complement(39435..40337)
                     /locus_tag="VBC52_000220"
     CDS             complement(39435..40337)
                     /locus_tag="VBC52_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEO6881828.1"
                     /translation="MKIDDLRKSDNVEDRRSSSGGSFSSGGSGLPILQLLLLRGSWKT
                     KLVVLIILLLLGGGGLTSIFNDSSSPSSYQSQNVSRSVDNSATREQIDFVNKVLGSTE
                     DFWSQEFQTQGFGNYKEPKLVLYTNSIQTGCGIGESASGPFYCSADKKIYLDISFYNE
                     LSHKYGATGDFAMAYVIAHEVGHHIQTELGIMDKYNRMRHGLTKKEANALNVRLELQA
                     DYYAGVWAHYIRGKNLLEQGDFEEAMNAAHAVGDDTLQKETYGKLVPDSFTHGTAEQR
                     QRWFNKGFQYGDIQHGDTFSVEHL"
     gene            40515..43748
                     /gene="rexB"
                     /locus_tag="VBC52_000221"
     CDS             40515..43748
                     /gene="rexB"
                     /locus_tag="VBC52_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEO6881829.1"
                     /translation="MKLLYTDINHDMTEILVNQAAHAAEAGWRIFYIAPNSLSFEKER
                     AVLENLPQEASFAITITRFAQLARYFTLNQPNQKESLNDIGLAMIFYRALASFEDGQL
                     KVFGRLKQDASFISQLVDLYKELQTANLSILELKYLHSPEKFEDLLAIFLVVSDLLRE
                     GEYDNQSKIAFFTEQVRSGQLDVDLKNTILIVDGFTRFSAEEEALIKSLSSRCQEIII
                     GAYASQKAYKANFTNGNIYSAGVDFLRYLATTFQTKPEFILSKWESKSGFEMISKNIE
                     GKHDFTNSSHILDDTAKDCITIWECINQKDEVEHVARAIRQKLYQGYRYKDILVLLGD
                     VDSYKLQLSKIFEQYDIPYYFGKAETMAAHPLVHFMDSLSRIKRYRFRAEDVLNLFKT
                     GIYGEISQDDLDYFEAYISYADIKGPKKFFTDFVVGAKKFDLGRLNTIRQSLLTPLES
                     FVKTKKQDGIKTLNQFMFFLTQVGLSDNLSRLVGQMSENEQEKHQEVWKTFTDILEQF
                     QTIFGQEKLNLDEFLSLLNSGMMQAEYRMVPATVDVVTVKSYDLVEPHSNQFVYALGM
                     TQSHFPKIAQNKSLISDIERQLINDANDTDGHFDIMTQENLKKNHFAALSLFNAAKQE
                     LVLTIPQLLNESEDQMSPYLVELRDIGVPFNHKGRQSLKEEADNIGNYKALLSRVVDL
                     YRSAIDKEMTKEEQTFWSVAVRYLRRQLTSKGIEIPIITDSLDTVTVSSDVMTRRFPE
                     DDPLKLSSSALTTFYNNQYKYFLQYVLGLEEQDSIHPDMRHHGTYLHRVFEILMKNQG
                     IESFEEKLNSAINKTNQEDVFKSLYSEDAESRYSLEILEDIARATATILRQDSQMTVE
                     SEEERFELMIDNTIKINGIIDRIDRLSDGSLGVVDYKSSAQKFDIQKFYNGLSPQLVT
                     YIDAISRDKEVEQKPPIFGAMYLHMQEPRQDLSKIKNLDDLVTKNHQALTYKGLFSEA
                     EKEFLANGKYHLKDSLYSETEIAILQAHNQSLYKKASETIKSGKFLINPYTEDAKTVD
                     GDQFKSITGFEADRHMARARALYKLPAKEKRQGFLTLMQQEEENDDL"
     gene            43738..47361
                     /gene="addA"
                     /locus_tag="VBC52_000222"
     CDS             43738..47361
                     /gene="addA"
                     /locus_tag="VBC52_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEO6881830.1"
                     /translation="MTFKPFLNPEDIAVIQTEEKNSDKKQKRTPEQIEAIYTFGNNVL
                     VSASAGSGKTFVMVERILDKLLRGVPIDSLFISTFTVKAAGELKERLEKKINESLKSA
                     ESDDLKQFLTQQLVGIQTADIGTMDAFTQKIVNQYGYTLGISPIFRILQDKNEQDVIK
                     NEVYADLFSDYMTGKNAASFIKLVKNFSGNRKDSKAFREMVYKVYAFSQSTDNPKRWM
                     QTVFLKGAQTYTDFEAIPDQEVSSLLNVMQTTANQLRDLTDQEDYKQLTAKGVPTANY
                     KKHLKIIENLVHWSQDFNLLYGKKGLTNLARDITNVIPSGNDVTVAGVKYPIFKQLHN
                     RIVGLKHLEVIFKYQGESLFLLELLQSFVLDFSEQYLQEKIQENAFEFSDIAHFAIQI
                     LEENHDIRQLYQDKYHEVMVDEYQDNNHTQERMLELLSNGHNRFMVGDIKQSIYRFRQ
                     ADPQIFNDKYKAYQDNPSQGKLIILKENFRSQSEVLDSTNSVFTHLMDEEVGDILYDE
                     SHQLKAASPRQQERHPNNKTQVLLLDTDEDDIDDSDSQQYDISPAEAKLVAKEIIRLH
                     KEENVPFQDITLLVSSRTRNDGILQTFDRYGIPLVTDGGEQNYLKSVEVMVMLDTLRS
                     IDNPLNDYALVALLRSPMFGFNEDDLTRIAIQDVKMAFYHKVKLSYHKEGHHSDLITP
                     ELSSKIDHFMKTFQTWRDFAKWHSLYDLIWKIYNDRFYYDYVGALPKAEQRQANLYAL
                     ALRANQFEKTGFKGLSRFIRMIDKVLENENDLADVEVALPQNAVNLMTIHKSKGLEFK
                     YVFILNIDKKFSMVDITSPLILSRNQGIGIKYVADMRHELEEEILPAVKVSMETLPYQ
                     LNKRELRLATLSEQMRLLYVAMTRAEKKLYLVGKASQTKWADHYDLVSENNHLPLASR
                     ETFVTFQDWLLAVHETYKKQELFYDINFVSLEELTDHHIGMVNPSLPFNPDNKVENRQ
                     SEDIVRAISVLESVEQINQTYKAAIELPTVRTPSQVKKFYEPILDIEGVDVMETITKT
                     SVDFKLPDFSTSKKQDPAALGSAVHELMQRIEMSSHVKMEDIQKALTEVNAETSVKAA
                     IQIEKINYFFQETSLGKYIQEEVEHLHREAPFAMLKEDPESGEKFVVRGIIDGYLLLE
                     NRIILFDYKTDKFVNPLELKERYQGQMALYAEALKKSYEIEKIDKYLILLGGKQLEVV
                     KMD"
     gene            47374..48291
                     /locus_tag="VBC52_000223"
     CDS             47374..48291
                     /locus_tag="VBC52_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6881831.1"
                     /translation="MIVEQKFGNGFTWINIEAEQLRTETSEIQAKYLDSEIITYALDD
                     YERAFMECSHIKGKEVLTIIFNTIDLKQKESYYETVPMTFCLSHDRLITVTRSRNSYM
                     LELLQKYLDRNPDVSPKKFLFAALTLITKQYFNVVSKIDREKDILNRQLREQTTNKRL
                     LAMSDLETGSVYLLTAANQNALVLEQLDVHPSQRFNSEVEKEQLSDALIEAHQLVSMT
                     QLNSQVLSQLSSTFNNVLNNNLNENLTGLNIISINLAIIAAITGFFGMNIPLPLTESR
                     SSWLIVIATSVLLWVIIAQILKRVFNLRK"
     gene            complement(48324..49700)
                     /gene="mnmE"
                     /locus_tag="VBC52_000224"
     CDS             complement(48324..49700)
                     /gene="mnmE"
                     /locus_tag="VBC52_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEO6881832.1"
                     /translation="MSITKEFDTIAAISTPLGEGAIGIVRISGTDALKIASKIYRGKD
                     LSAIQSHTLNYGHIVDPDKNEILDEVMLGVMLAPKTFTREDVIEINTHGGIAVTNEIL
                     QLILRHGARMAEPGEFTKRAFLNGRVDLTQAEAVMDLIRAKTDKAMDIAVKQLDGSLK
                     TLINNTRQEILNTLAQVEVNIDYPEYDDVEEMTTTLMREKTQEFQALMENLLRTARRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREEKAIVTDIEGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRDTDDIVEKIGVERSKKALEEADLVLLVLNSSEPLTLQDRSLLELSKESNRI
                     VLLNKTDLPQKIEVNELPKNVIPISVLENENIDKIEERINDIFFDNAGMVEHDATYLS
                     NARHISLIEKAVDSLKAVNEGLELGMPVDLLQVDMTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     gene            49828..51738
                     /locus_tag="VBC52_000225"
     CDS             49828..51738
                     /locus_tag="VBC52_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6881833.1"
                     /translation="MIILQGNKIERSFSGDVLFDNINIQVDQRDRIALVGRNGAGKST
                     LLKILVGEEAPTKGEINKKRDLSLSYLAQDSRFQSENTIFQEMLQVFDSLREVEKRLR
                     ELELQMGQVSGSDLEQLMKTYDILSEEFREKGGFTYESDIKAILNGFKFNSDMWEMPI
                     SELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIDTIAWLENYLVNYQGALIIVSHD
                     RYFLDKVATVTYDLTTHSLDRYVGNYSKFMDLKAEKIATEEKNFEKQQKEIAKLEDFV
                     QRNIVRASTTKRAQARRKQLEKMERLDKPNVEQKSANMTFHAGKVSGNVVLTLENAAI
                     GYEGVSLSEPIDLDVKKFDAIAIVGPNGIGKSTLIKSLVGQIPFIKGEAKLGANVETG
                     YYDQSQSNLTKTNTVLDELWDAFSTTPEVEIRNRLGAFLFSGDDVKKSVSMLSGGERA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIEFDGTLLFVSHDRYFINRVAT
                     KVLEISDKGSTLYLGDYDYYLTKKAELEELARLNEEEVSASKTEIDVTSDYETQKANQ
                     KEFRKITRRVVEIEARLEVLENDENNINGLMLETNDIGKLSDLQKELESIQEEQLLLM
                     EEWENLNMRLDL"
     gene            51888..52856
                     /locus_tag="VBC52_000226"
     CDS             51888..52856
                     /locus_tag="VBC52_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEO6881834.1"
                     /translation="MVTLSKEQHLDMFLKMQRIRDVDMKFNKLVRRGFVQGMTHFSVG
                     EEAASVGAIQDLTDSDIIFSNHRGHGQTIAKGIDIGGMFAELAGKATGTSKGRGGSMH
                     LANLEKGNYGTNGIVGGGYALAVGAALTQQYEGTDNIVIAFSGDSATNEGSFHESVNL
                     AAVWNLPVIFFIINNRYGISTDITYSTKIPHLYMRADAYGIPGHYVEDGNDLMAVYEK
                     MHEVINYVRSGNGPAIVEVESYRWFGHSTADAGVYRTKEEVDSWKAKDPVKRYRAYLI
                     ENEIATEEELAAIEAQVIKEVEEGVKFAEESPFPDMSVAFEDVFVD"
     gene            52931..53929
                     /locus_tag="VBC52_000227"
     CDS             52931..53929
                     /locus_tag="VBC52_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEO6881835.1"
                     /translation="MSETKVMALREAINVAMSEEMRKDEKVFLMGEDVGVYGGDFGTS
                     VGMLEEFGAKRVRDTPISEAAIAGSAIGAAQTGLRPIVDLTFMDFVTIAMDAIVNQGA
                     KTNYMFGGGLSTPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGLKVVAPGTVNESKAL
                     LKSSILDNNPVIFLEPKALYGKKEEVNMDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVMQAAEEVAEEGINVEVVDPRTLIPLDKELIIDSVKKTGKLILVNDAYKTGGFTGEI
                     ATMVAESEAFDYLDHPIVRLASEDVPVPYSRVLEQGILPDVAKIKDAIYKVVNKGK"
     gene            54056..55444
                     /locus_tag="VBC52_000228"
     CDS             54056..55444
                     /locus_tag="VBC52_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEO6881836.1"
                     /translation="MAVEIIMPKLGVDMQEGEILEWKKQVGDVVNEGDVLLEIMSDKT
                     NMEIEAEDSGVLLKITHGNGDVVPVTETIGYIGAEGEEVTEASSSENTSVEENATQVT
                     SEPEKVEETSEPSVPAATSGEKVRATPAARKLAREMSIDLALVSGTGANGRVHREDVE
                     NFKGAQPRITPLARRIAEDQGVDIAEITGSGIRGKIVKNDVLAAMSPQAAEAPVETKA
                     TPTTEEKQLPEGVEVIKMSAMRKAISKGMTNSYLTAPSFTLNYDIDMTEMMALRKKLI
                     DPIMAKTGLKVSFTDLIGMAVVKTLMKPEHRYLNASLINDAQEIELHNFVNIGIAVGL
                     DDGLIVPVVHNADQMSLSDFVIASKDVIKKTQEGKLKSAEMSGSTFSITNLGMFGTKT
                     FNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRIVDGMNGAKFMVDL
                     KNLMENPFGLLI"
     gene            55504..57261
                     /gene="lpdA"
                     /locus_tag="VBC52_000229"
     CDS             55504..57261
                     /gene="lpdA"
                     /locus_tag="VBC52_000229"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEO6881837.1"
                     /translation="MAFDVIMPKLGVDMQEGEILEWKKNEGDTVNEGDVLLEIMSDKT
                     NMEIEAEDTGVLLKIVHQAGDVVPVTEVIAYIGEEGEEVGTSSPSADATITAEDGQSV
                     SGPAAPSQETVAAATPKEELAADEYDIVVVGGGPAGYYAAIRGAQLGGKIAIVEKTEF
                     GGTCLNVGCIPTKTYLKNAEILDGLKVAAGRGINLASTNYAIDMDKTVAFKNSVVKTL
                     TGGVRGLLKANKVEIFNGLGQVNPDKSVVIGDKVIKGRNVVLATGSKVSRINIPGIES
                     PLVLTSDDILDLREIPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAMDKE
                     VSLELQKILAKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVADKALLSIGRVPQMN
                     GLENLEPELEMERGRIKVNAYQETSIPGIYAPGDVNGTRMLAHAAYRMGEVAAENALG
                     GNKRKAHLDFTPAAVYTHPEVAMVGMTEEQAREQYGDILVGKNSFTGNGRAIASNEAH
                     GFVKVIAEPKYKEILGVHIIGPAAAELINEASTIMENELTVYDVAQSIHGHPTFSEVM
                     YEAFLDVLGEAIHNPPKRK"
     gene            57359..58348
                     /locus_tag="VBC52_000230"
     CDS             57359..58348
                     /locus_tag="VBC52_000230"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO6881838.1"
                     /translation="MKYIVNTSNDPAYNVALEAYAFQKLTDIDEIFILWINEPAIIIG
                     RHQNTIQEINKEFIDKNGIHVVRRLSGGGAVYHDLNNLNYTIISNNTQEGAFDFQTFS
                     KPVIDTLAKLGVKAEFTGRNDLEINGQKFAGNAQAYYKGRMMHHGCLLFDVDMSVLGQ
                     ALKVSKDKIESKGIKSVRARVTNIVDHLSDKITVQEFSDAILAQMKEEYPEMDEYVLS
                     DAELSEIQAMRDNQFATWDWTYGKAPEYTIERGVRYPAGKITTYANVENSTIKSVKIF
                     GDFFGVKPVDDIEKMLEGVRYDYKDVLAALKTVDTSQYFSRMTPEEITKAIVD"
     gene            complement(58456..59241)
                     /locus_tag="VBC52_000231"
     CDS             complement(58456..59241)
                     /locus_tag="VBC52_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEO6881839.1"
                     /translation="MTYTSLKSPTTKDYKYTLNVAHLYGNLLNTYGDNGNILMMKYVG
                     EKLGCQMTFDIVSLEDRFDPNYYQMAFFGGGQDYEQAIVARDLPSKKEDINKFIQNNG
                     VVLAICGGFQLLGQYYIQANGERIEGIGVMGHYTLNQNNNRYIGDIKIHNDEFNETYY
                     GFENHQGRTFLSEDEKPLGTVIYGNGNNKEDGTEGVHYKNVFGSYFHGPILSRNANLA
                     YRLVATALRNKYGKEIVLPSYEEILSLEIPEEYGDVKSKADFE"
     gene            complement(59241..60584)
                     /locus_tag="VBC52_000232"
     CDS             complement(59241..60584)
                     /locus_tag="VBC52_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEO6881840.1"
                     /translation="MKINTALGVAAGKSAHYLLSKMGRGSTYPGSLALKFDKDILDTI
                     AKDYEIVVVTGTNGKTLTTALTVGILKEAFGQVVTNPSGANMITGIVSTFLTAKKSKS
                     GKKIAVLEIDEASLPRITQYIKPSLFVFTNIFRDQMDRYGEIYTTYQMILDGAANAPQ
                     ATILANGDSPLFNSKSVTNPVQFYGFNTDKHEPRLAHYNTEGILCPKCQAILTYRLNT
                     YANLGDYTCPNCDFERPNLDYALTRLTHLTNTSSGFVIDGQQYNINVGGLYNIYNALA
                     AVSVAEYFGVEPSQIKDGFDKSRAVFGRQETFTIGNKKCTLVLIKNPVGASQALDMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDANFETILTMNIPEIFAGGVRHSEIARRLRVT
                     GYDEKRIKQADKLQDIMTMIEQQETEHAYILATYTAMLEFREILANHNAIRKEMN"
     gene            60724..61575
                     /locus_tag="VBC52_000233"
     CDS             60724..61575
                     /locus_tag="VBC52_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEO6881841.1"
                     /translation="MDIFSAIDSKFWASIMENPWMILIHLMDIIIVAVLIYKFIKALA
                     GTKIMSLIQGVILFIIIRFVSEWIGLTTITFLMNQIVTYGVIAGVVIFAPEIRTGLEK
                     FGRTPQLFTQRSQLSSDEKLVDALVKAVAYMSPRKIGALISIERTQTLQEYIATGIPL
                     DADISSELLINIFIPNTPLHDGAVIVKDKKIATACSYLPLSESSSISKEFGTRHRAAI
                     GLSENSDALTVIVSEETGGISVALKGEFLHDLSKDSFEAILRTQLIQNQEENSKLAWY
                     NQLLRRK"
     gene            61578..62537
                     /locus_tag="VBC52_000234"
     CDS             61578..62537
                     /locus_tag="VBC52_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEO6881842.1"
                     /translation="MKKFFTNKFWLGVVSLFLAILLFLTATATSMNHQDNSKIAGASE
                     TYTHTLTDVPIDIKYDSDDYFISGYSYGADVYMSSVNRVKLDSEINEDTRKFKVVADL
                     TNMKPGTHKVPLKVVNLPSGVNATVSPTTITVTMGKKKTKEFPVYGHVNDKQIKAGYA
                     VDKMSVDVSKVKVTSDESIIDRIDHVAANIPDDKVLDDDFNKTVTLQAVTADGTVLAS
                     IIHPSKATLSVKVKKLTKTVPINLIPVGQFSDSISKINYKLSQEKAVISGTKEALEAI
                     SVINAEVDISDVTKNTEKKINLSANNVSVDPAQVTVQLTTTKK"
     gene            62591..63943
                     /locus_tag="VBC52_000235"
     CDS             62591..63943
                     /locus_tag="VBC52_000235"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEO6881843.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETDRPRV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGSDGFKLDDDRELEIEALLDAKEDTLPRPSAQGLGTLVDYPEGLRKY
                     EKFMESTGIDLEGMKVALDTANGAATASARNIFLDLNADISVIGDQPDGLNINDGVGS
                     THPEQLQSLVRENGSDIGLAFDGDSDRLIAVDENGEIVDGDKIMFIIGKYLSDKGQLA
                     QNTIVTTVMSNLGFHKALDREGIHKAITAVGDRYVVEEMRKSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLTKVMKETGKKLSELASEVTIYPQKLVNIRVENNMKDKAMEVPAI
                     AEIIAKMEEEMDGNGRILVRPSGTEPLLRVMAEAPTNEAVDYYVDTIADVVRTEIGLD
                     "
     gene            64066..64437
                     /locus_tag="VBC52_000236"
     CDS             64066..64437
                     /locus_tag="VBC52_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEO6881844.1"
                     /translation="MTKEAELLWAKHKYLVLSKSQKIYLDIRQTLKSPNCTVLDVQSL
                     IDQAVLLEESPSQVTNAYMHIWGYFKNKAERQEKEEFLTLLEKYRKTGYQRRKLLAFL
                     KQLLAKYPNSYLQNSSIFEEE"
     gene            64462..64842
                     /locus_tag="VBC52_000237"
     CDS             64462..64842
                     /locus_tag="VBC52_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881845.1"
                     /translation="MRLWHQDIIELLPRQQLLGQHRECCALRGNGWGRKHETVDYVFR
                     YSPYRLFAYHQLVMEEMMERGYRVSKEWLIAEYRGMKCPRYDTLNPVDLETPIYPEHN
                     QDYLQECLWNLKAKGIDLPINKIK"
     gene            64934..66064
                     /locus_tag="VBC52_000238"
     CDS             64934..66064
                     /locus_tag="VBC52_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEO6881846.1"
                     /translation="MLKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDAYLQVLIRE
                     FRSYDITELRTLYIGGGTPTSISAVQLDYLLTELSRDLNLNTLEEFTIEANPGDLTVD
                     KIEVLQKSAVNRVSLGVQTFNDKHLKRIGRSHNEAQIYSTIDALKTAGFQNISIDLIY
                     ALPGQTMDDVRSNVAKALSLNIPHLSLYSLILEHHTVFMNKMRRGKLHLPTEDLEAEM
                     FEYIISEMERNGFEHYEISNFTKPGFESRHNLMYWDNVEYYGVGAGASGYLDGIRYRN
                     RGPIQHYIKGVSEGNARLSEEVLSKNEMMEEELFLGLRKKEGVSIGKFEQKFGTSFEK
                     RYGQIVQELQSDGLLKENNGFIQMTKKGLFLGDTVAEKFIVE"
     gene            66068..66805
                     /locus_tag="VBC52_000239"
     CDS             66068..66805
                     /locus_tag="VBC52_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEO6881847.1"
                     /translation="MGLLYRETYEVPFYESDTNHYMKLPQLLALALQISAKQSLKLGI
                     GDDIVFKRYGLVWVVTDYIIDIERLPKHAEKIVIETEAKAHNKLLCYRYFYIYGEDGQ
                     KIITISSAFVLMDFKTRKIHPVLDDITSIYQSQRIKKVIRGPKYHPIGDSKVKQYHVR
                     YFDLDMNGHVNNSKYLEWMYDVLDLDFLSSHIPKKIDLKYIKEIQYGTDIKSHWYQDG
                     LVTRHDIIGGDAIHAQARIEWQEKKED"
     gene            66806..67576
                     /locus_tag="VBC52_000240"
     CDS             66806..67576
                     /locus_tag="VBC52_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEO6881848.1"
                     /translation="MAYKGYLIDLDGTIYKGKSRIPAGERFIERLQEKGIPYMLVTNN
                     TTRTPESVQEMLRGFNVETPLETIYTATMATVDYMNDMNRGKTAYVIGEEGLKKAIAD
                     AGYVEDTKNPAYVVVGLDWNVTYDKLATATLAIQNGALFIGTNPDLNIPTERGLLPGA
                     GSLNALLEAATRIKPVFIGKPNAIIMNKALEILNIPRNQAVMVGDNYLTDIMAGINND
                     IDTLLVTTGFTTVEEVPDLPIQPSYVLASLDEWTFNEG"
     gene            67566..>68195
                     /locus_tag="VBC52_000241"
     CDS             67566..>68195
                     /locus_tag="VBC52_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEO6881849.1"
                     /translation="MKDKLLVVLTWIWIISLATLATIYIAWLIYPIEIQFLKLEKVVY
                     LKAETIYYNFNKLMIYLTHPFISDLNMPSFPSSEDGLKHFADVKYLFTLAHGLFVILT
                     FPVIYFLRRGWKQKSIFLYEGFFKIAIMLPIFIVVCAFLLGFDQFFTLFHEVLFPGDS
                     TWQFNPLTDPVIWILPETFFLHCFIIFLLIYETITIILLIIGRKHLKLRK"
BASE COUNT        22289 a        10632 c        13763 g        21511 t
ORIGIN      
        1 taaaaaagaa ttagtaaatg cagagttaac aaaagtaatt tctaatgaca atattttatt
       61 agacatgcaa gcaattagtt ccaattatgg gtctactaaa aagtttgttt attcagtcaa
      121 aagattacct tacgtcccgc aagtaattat gacaacaacg tctaatgtac ttatgcctca
      181 aaagcaagtt gaaaaagtga agctactcac cccatttaca atttctaata aagaagtatt
      241 gaatcatgac tcactagtag aaaatgatgc tcaaaaacaa aaagtagaac aggaaaaaac
      301 taaatcgcta gctcctcaaa aaggggctgt aaaagaacaa actgagcaaa aagtatctgg
      361 aaatactcaa gagatagaaa agaaatctga aactgtggca actccacaac aaagttcagt
      421 tgcgcaaact tctgtccaac agccggctcc ggttcaatca gttgttcaag aatccaaagc
      481 ttctcaagag gagattaatg cagcacacga tgctatttcg gcgtataaat caacagtcaa
      541 tattgctaat acagccggtg taacaactgc ggaaatgacc acgctcatta atactcaaac
      601 ttctaatctt tctgatgttg agaaagcttt aggaaataat aaggttaata atggtgcagt
      661 caatgtattg agagaagata cagctcgtct tgagaatatg atttggaatc gtgcttacca
      721 agctattgaa gaattcaacg tcgctcgtaa tacttataat aaccaaatca agacagaaac
      781 agttccagtt gataatgata ttgaagctat tttagcaggt tctcaagcta aaattagcca
      841 tttggacaat cgtatcggag cgcgccacat ggatcaagct tttgtagcta gtttattaga
      901 agttactgag atgagtaaat caatctcatc gcgtataaaa gagtagacac tgctatcaag
      961 gcgatcttaa acttttgtat taaactaacc taaaagatag aaagagacta atatgaaaaa
     1021 aataacaact ttaatcttag ctagtagctt attactagtt gcaacgacat cggttaaagc
     1081 tgatgataac tttgaaatgc caacgcgtta tgttaaaatg agtgaaaaat caaaagcatt
     1141 ttatcaaaga ctacaagaaa aacaacgtaa ggcacatact actgtgaaga cttttaataa
     1201 ttcagaaata aggcatcaac tacctcttaa acaagaaaag gctagaaatg atatctacaa
     1261 tttaggcatt cttatttctc aggagtctaa agggttcatc caacgtattg ataatgccta
     1321 ttctttggaa aatgtctcag atattgttaa tgaagctcag gctttgtata aacgtaacta
     1381 tgatttattt gaaaaaatca aatctacacg tgataaggtt caagtcttac ttgcatcgca
     1441 tcaagataat acagacttaa aaaactttta tgctgagttg gacgatatgt atgaacatgt
     1501 ttatctcaat gaaagtagag tggaggcgat aaacagaaat atccaaaaat ataattagtt
     1561 tctaaactaa caaacattcc taaatataag acataaaacc ctactcattg attagtgagt
     1621 agggttttac tgttttaaat agctttctga tcagaatgta agccttgtca tttcaaagga
     1681 actatgttat tattcttaag taaattaaat aggacatttg gggtgcgtaa cagctgagat
     1741 tatacccatt gaacttgatg cagttagtac tgtcgaaggg aaatgtgtgc cattacaagt
     1801 tttttgtggt ggaaaagggc gtagtgtctt cttagtagac agctagctct agttaatcag
     1861 ataataaact ccgtttgctt gcagatggag ttttttgttg tcaaaatttt ttgtaggttg
     1921 ttgcccctta tctgtcatag agaaggagtt atgatgacta atcataaaac aaaaaaatta
     1981 acggttttag caattatgat tgctttagat gtttttttaa cgcccatttt tagaatagaa
     2041 ggcatggcct ttatgtctag tgtggttaat attatcgctg gagttataat gggaccaatc
     2101 tatgcattag ttatggcact tgtaacggct tttattcgta ttttaactca agcaggagta
     2161 gcatctgttg cacctttagc tattctagga gctggtccag gagctttctt agcgggttgg
     2221 ttttatcggt tttatgcgaa agactggatg tcttgggtgg gtgagtttat aggaacaggg
     2281 ataattggag cacttttatc agctccaatt atggtctggt tttggacttg gacagcggat
     2341 ggaaatcacg aagtgttagc taaagcatct gcagctcaat ccatttttct ttttatgcca
     2401 aggtttatag gggcgactat aattggcggt gttattgctt tgttgctact tcagggatta
     2461 aagcgggttc ctgtatttat gacaatacaa gcattattta aaggagaaaa agtaaaatga
     2521 aattgagttt aaaagatgta ttattgattg ctctattagc ggttgttctg ggcgttgttt
     2581 actttggagc aggctatatt tcaaatgctt ttgtaccttt tgtaggccct atagcgcatg
     2641 aagttattta tggtatttgg tttgtggcag gtccaatggc tctttatata ttgaggaaac
     2701 caggaaccgc tattgtagca gaattgttag ctgctttgat agaggtttta attggatcaa
     2761 tttatggacc aagtgtactc gttattggta cccttcaagg tttagggtca gaattaggct
     2821 ttacactatt taggtatcat aattacaagt tgccagcttt tatattgtct gctattttga
     2881 catcaatttt tagttttgca tggagttttt atgccaatgg acttagtgcg ttttcatttt
     2941 catataacat tctgatgctt atagtaagaa ctgtttcatc tattattttc ttcttattaa
     3001 caaagaatat atgcgaccaa ttgcatagaa gtggtgtact gaatgcttat gggattcatg
     3061 aaaggaagta gcctatgcta tctgttgaaa aattagcttg cacccatggc gatagtcatt
     3121 atttatttga tgaagtgact tttagtctta acccaggaga acgaatttta atcagtggct
     3181 attcagggtg tggtaagagt acattggctc ttttactatc agggttaaaa gaaagtggga
     3241 aaggtcaagt tttattaaac ggctcattaa tagaaccaag cgatgtgggg tttctttttc
     3301 aaaatcctga tttgcaattt tgtatggata ctgtggcgca tgagttgtat tttattttag
     3361 aaaatttaca aatagaaccg gagcaaatgc aagatagaag tgagtttgta ttggcacaag
     3421 ttggtttaaa aggatttcag aatcggttga tttatacttt atctcaaggt gaaaaacagc
     3481 gattagcatt ggcgaccatt tttttaaaat ctcctaaact tattatttta gatgaagctt
     3541 ttgcgaattt ggatcaagag agtgccagtc aactattaca attagttctt aattatcaag
     3601 caaacaatca atcaatgctg attgtaatcg atcatttgat tacttattat caagatataa
     3661 tggatcacta tttttggttg gaaaaacggt tgacaagggt caattttgat tatatgctca
     3721 atcgtttgaa tgtcttcgaa ttagaaaaga aaagtcataa tacaggtgac aaactgttat
     3781 caattaaaga tttccaagtt aaactttcaa aaaacaaatt catctcttac cttgattttg
     3841 atttagcaag tggggagagg ttgtgcttag atggaccaag tggcgtgggg aagtctagtt
     3901 tgtttatggg attgttaggg ctttatagga ctaaagggaa aaaacaattt actcatagaa
     3961 agcaaattcc tataagcttt ttatttcaaa atcccttaga ccaatttatt ttttcaacag
     4021 tttatgacga aatttttcag gtttgcaagg atagtaacaa agcgagggat attttggaaa
     4081 ccataaatct ttgggataag aaacagtttt caccatttca actaagtcaa ggccaacaga
     4141 ggcgattagc cattggcagt attttagcta gtgatagtaa acttctatta ttagatgaac
     4201 caacttatgg acaagatgcc tatcacgcta atatgattac aactcttctt ttgagttatt
     4261 gtcacaaaaa tcattgtggg gttattttta ctagccatga tcctcactta aaagagtgtt
     4321 ttgcaacacg tttcttggag gtgtcatctt gttcaatttg aaaaactatc atccagctat
     4381 taccttccta tgtttattaa taccaacctt ttcgtttagt ttcacattga ggttaagaac
     4441 gtctttgtta tttctaatta tagtagtaac actgcaatgc ttcgtaaagg tttctttaaa
     4501 aacttgggcg aaggtcaatc tgatatcatt tgtgatggga ctctctttat ttctagggac
     4561 gtatttttgg ggaaaattac cccatcaatt cgtcttagct agtctagtgg cctgtcgtcc
     4621 gcttattttt atgaacgtgg gtttgttatt tcatgcatca cattctaatt atgattttat
     4681 agaatctctt tatcaaactt tcaaagtgcc tagtcatttt gcatatggta tttttgcggt
     4741 ctttaatctt ctaccattaa ttaaattaca ataccaacgt aatcgactcg cattccgact
     4801 gaagaatcag gtaacttggg cattatcgcc tagactaata ttgtcagttt tattaaagac
     4861 gatatattgg gtagaacagt tagagttagc aatgctttct aaaggttttg aagtgggtaa
     4921 agagcgaaca catgcttcaa cttatcccgt tcgttttcga gattatagtt tacttggtat
     4981 gagtatttta ttgtctatag gaatgatttt taaataagta caattagttt agtaatacaa
     5041 ttgtgcattt atatagaatg agcgtgttct gctcatgaga tcataacaaa ggagattact
     5101 atgttaaaag ataagatgat tgctaaagga gcaaaatata tccaatccat ctatcaagat
     5161 ccatttattc aaggaattat aaaagggcgt ttagaccatg acgtaatctg ccattattta
     5221 caggcagata atatctacct tgggaaattt gctgatattt atgccctgtg cttggcaaaa
     5281 tctgataatt tacgagataa acaatttttc cttgaacaaa ttgactttac attaaatcgg
     5341 gagttagcag atggtgaagg cccacatcaa gccttggccg cttatactaa tcgatcttat
     5401 caggatatta tcgaaaaggg tgtatggtac ccgtcagcag atcattacat taaacatatg
     5461 tattttcatt tttacgaaaa tggtattgct ggtgctttag ctgcaatgag cccatgtcca
     5521 tggatttacc atcaattggc taaaaaaata attgaggaga accagttttt gaatggtaac
     5581 ccttttaata attggataac tttttatgct aacgatacag ttgaagagtt aatggaaaac
     5641 tattttcgta tgatggatta ttatgcgcag aatttgtcaa aagagaagca agctgatttg
     5701 gtagatgctt ttgtgaaatc ttgtcagcat gagagacgtt tttttcaaat ggcaataaat
     5761 caagaaaaat gggaggatta atatgactta tttagcacca gttttaacta ttgcaggaac
     5821 tgatcctagc ggtggagcag ggattatggc ggatttaaag acatttcaag ctcgtcggac
     5881 ttacgggatg gctgttgtga ctagtgttgt tgctcagaac acttgtgggg ttagaggcgt
     5941 tcagcatata gaaacagcta tcattgatca acaactagct tgtgtctatg atgatattaa
     6001 gccaaaggcg gttaaaacag gaatgttagc ggagagggaa acaattagtt tagtcgcttc
     6061 atatttaaaa aaatatccgc aaccttatgt attagatcct gttatggtgg caactagcgg
     6121 acatcgttta attgattcag atgccgtaga agcattaaaa gaagacttat tgccattagc
     6181 aactattatc acaccaaatt taccagaagc tgaggtttta gtgggatatg acttatcaga
     6241 tgaagtaagt attattaaag caggttacga tattcaaaaa caatattctg ttagaaatgt
     6301 tcttataaag ggaggacatc tagatggctt ggctaaagat tatttatttt tggaaaaaga
     6361 agggttgata actcttagta atcaacgtat caatactatt catactcatg ggactgggtg
     6421 tacatttgca gcagtcgttg cagcggagtt agctaaaggg caatcaattc taaacgcggt
     6481 aagtaccgct aaatcattta ttacttcagc tatcgaaaca gcgcctgagt tgggccttgg
     6541 aaacggtcct gtcaatcata ctagctatca aggagactaa aatgtattta gaacaattaa
     6601 aagaggtaaa tcctttaacc atttgtatca ctaataatgt ggtcaaaaac tttacagcca
     6661 atggtttatt agctttagga gcttctccgg ctatgagcga gtgcatagag gacttagaag
     6721 acttgttaaa agttgcagat gcattattaa ttaatatagg aacactaaca aaagaaagtt
     6781 ggcaacttta ccaagaagca atcaaaatag caaataaaaa ccaagttcca gttgtattag
     6841 atcctgtagc ggcaggagca agtcgttttc gattagaagt gtcacttgat ttattgaaga
     6901 attattctat tagtttactt agaggaaatg gtagtgaaat cgctgctttg attggtgaaa
     6961 aacaagcgtc taaaggggca gatggcggaa aagtggctga tttagaatca attgctgtaa
     7021 aagccaatca agtttttgat gtgccagtgg ttgtcacagg agaaactgat gctatcgctg
     7081 ttagaggaga agttcgctta ttacaaaatg gtagtccttt aatgccactt gttacaggaa
     7141 ctggatgtct attaggggca gtattggctg cttttattgg tagttctgac agaagcgatg
     7201 atcttgcttg tctgacagag gccatgactg tttataatgt tgctggagaa attgcagaga
     7261 aggtggcaaa aggtaagggg gtgggaagtt tccaagttgc atttttagat gccctttccc
     7321 agatgaaatc agaaatgatt atggacaaat aggtggtgtc agtagatgaa agatacattg
     7381 aaattatatt ttgtttgtgg aactgtggat tgctctagga agaatatatt gactgttgtt
     7441 gaagaagcct tacaagcagg tataactcta tttcaattta gagaaaaagg ttttacagca
     7501 ttacaaggta aggaaaaaat tgctatggct aagcaactcc aaatactttg taagcaatat
     7561 caagttcctt ttattattga tgatgacatt gacttagttg agttaattga tgctgatggt
     7621 cttcatattg gacaaaatga tttacctgtt gatgaggcac ggagacgttt accagataaa
     7681 atcatcggtt tatctgtgag tacaatggat gaatatcaga aatcacaatt atctgttgtt
     7741 gattacatcg gaattggtcc ttttaatccg acacaatcaa aagcagatgc taagcctgct
     7801 gttggcaata ggacaacaaa ggcagttaga gaaattaatc aagatattcc aatcgttgca
     7861 ataggcggaa tcactagtga ttttgttcat gatattatag aatcaggtgc agatgggatt
     7921 gctgttatat cggctatttc aaaagcaaat catattgtag atgcaaccag acaattgcgt
     7981 tatgaagttg aaaaagctct ggttaatagg caaaaacaca gtgatgtaat caaataaggc
     8041 tacatcgtta attataagaa tttttaagat attatatcat cacacaatac tggtatttga
     8101 aaaagaattt aaactatgat aatatagtag agttgaattc tagttaaaga aagatttgaa
     8161 agtatgcgta aaattattat taacggtggc aagcaactga cgggagaagt tgctgtatcg
     8221 ggtgccaaaa atagtgtagt tgccttgatt cctgccacta ttcttgctga tgatgttgtg
     8281 gtcttagatg gagttccggc tatttctgat gttgacagtt tagttgacat tatggaaact
     8341 atgggagcta agattaagcg ttatggtgag acgcttgaaa ttgatccatg tggcgttaaa
     8401 gatattccaa tgccttatgg caaaatcaat agtcttcgtg catcctatta tttctatgga
     8461 agcttattgg ggcgttatgg tcaagcaaca ttaggtttac ctggtggttg tgatttaggc
     8521 ccccgtccga ttgatttaca tttaaaagct tttgaagcga tgggagctag tgtttcttat
     8581 gaaggtgatt caatgcgtct agcaacaaac ggaaagcctt tgcaaggagc aaatatttat
     8641 atggatactg tcagtgttgg tgcgacaatt aacactataa tcgcagcagc taaggcaaat
     8701 ggccgtacgg ttattgaaaa tgctgcacgt gaaccagaaa ttattgacgt ggctactctt
     8761 ttaaataata tgggagctca tattcgtggt gctgggactg atgttattac aattgaaggt
     8821 gtgaaatcgc tccatggcac gcgtcatcaa gtaattccag atcgtattga agcaggaact
     8881 tatattgcta tggcagcagc tatcggacga ggaataaaag tgaccaatgt gttatatgaa
     8941 caccttgaga gtttcattgc taagttagat gagatgggag ttcgaatgac agttgaagag
     9001 gattcaattt ttgtcgaaga acaagaaagg ttaaaagctg tatccataaa aacctcacct
     9061 tacccaggat ttgctacaga tttacaacaa cctctaactc ctttattact aacagcagaa
     9121 ggaaacggga gtttattaga tactatctat gagaaacgtg ttaatcatgt tcccgaatta
     9181 gcacgtatgg gagctaatat ttctacccta gggggcaaaa tagtttatag tggtcctaat
     9241 caattatctg gtgcacctgt aaaagcgaca gatttgcgtg caggcgctgc tttagttatc
     9301 gctggcttaa tggcagaggg acgaactgaa attacgaata tcgagtttat tttacgtgga
     9361 tattctaata tcatcgagaa actaacatca ttgggtgcgg atatccaatt ggttgaggaa
     9421 tagtatatat aacaaagagt tgagaatatt tcttagctct tttcttcttg ttttagatta
     9481 agtcacttat aggtatttga aatctcttgt ggtataatat tatcatggat atatggacaa
     9541 atttaggacg gtttgcattc attgaaacag aacatgttaa cctacgtcct gttgcctaca
     9601 ctgataggga agctttttgg cgaatagctt caaaaaggac aaatttacaa tttatttttc
     9661 ctgttcaaac cagtaagaag gaaagtgatt ttcttttagt tcattctttt atgaaagagc
     9721 cattgggagt ttgggcgatt gaagataaag ttagtcataa aatgtttgga gttatccgtt
     9781 ttgaaaacat tgatttatct aaaaagacag ctgaaattgg ctattttttg aaagaaagtt
     9841 cttggggtca aggcattatg actgaatgtc taaagacact ttcttttttt gcttttcgag
     9901 agtttgggat ggacaaattg atcatagtaa cacataaaga gaatattgct agtcagaagg
     9961 ttgctttgaa ggcgcatttc aaacaatcta ggagttttaa aggaagcgac cgttacacac
    10021 gccgtataag agactatatt gagtttcaat taactagggg tgattatcgt tatgagtaaa
    10081 catcaagaaa ttttggagta cctagaaaat ttagctgttg gtaagagggt tagtgtacgc
    10141 agtatttcaa atcatttaaa ggtgagtgat ggcactgcat accgcgctat aaaagaggca
    10201 gaaaataggg gtattgttga gactagaccg cgtagtggga cagtacgtgt tgctcaaaaa
    10261 gcaaaggtta atattgaaaa actgacttat gctgaaattg caaggattag tgactcacaa
    10321 gttgttgcag gaatagaggg actcagcaag gagtttagta aattttctat aggtgcaatg
    10381 actcacagaa atattgaaaa atatttggtg caaggagggt tattgattgt tggcgaccgt
    10441 gatgagatac aacatttagc cttacaacat caaaacgcta tcttagttac aggtggcttt
    10501 aatgtttccc catctgtttg tcgtttagca gataaacttc aaattcccgt aatggtaaca
    10561 cactatgata cattcacagt atcaacgatg attaatcaca ctttgtcaaa tgccaaaatt
    10621 cgtactgatt taaagaccgt tgaacaagtc tatcagtcgc aaatggatta cggtttctta
    10681 gcgcaggatg ataccgtaaa agaatttaat cttcttgtta aacaaactaa aaatgttcgt
    10741 tttccaatag tcaaccaagc taatgttgtc gttggtgttg ttagtgttca agatattctt
    10801 ggaaaagata aagaagtaaa attggcaaca gtcatgtcaa aaaatatcat tgttgctaaa
    10861 cctcgaatga gtctagctaa tatcagtcaa aaaatgattt ttgaagattt gaatatgatg
    10921 ccagtagtat cggatgactt tgaattatta ggcgttatta caagacgaca agctgtagaa
    10981 aatttatcga tgtcgcaagg gacggattta tacacctata gtgatcaaat cttgtctaat
    11041 ttacaaattg aagatggtca cttttcgttt ttagtagaac ccgctatgat tgaccataca
    11101 ggtagcttaa cacaaggagt tttgacggag ttcttaaaag aaatttgtat tcgtgtctta
    11161 actcgtaagc atcaacgcag tattgttgtt aaacaaatga cactttattt cttgcaacct
    11221 gttcaaattg atgaaataat aatggtaacg ccaacaatta tttcagaaaa acggcgcgaa
    11281 gctacactcg atttagaatt aaaattagaa aataaaatta ttgctaaagc tatgatagcg
    11341 gtaaaaatta attagatata aggagaataa atagaaaatg attacattaa agtcagcacg
    11401 cgaaattgaa gcaatggata gagcaggtga ttttttagcc agcatccaca ttggacttcg
    11461 tgatattatc aaaccaggag ttgatatgtg ggaagttgaa gaatacgttc gtagacgttg
    11521 taaagaagaa aacgtattgc cattgcaaat cggggttgat ggtgcagtta tggattaccc
    11581 atatgctact tgttgtgggc ttaatgatga agtggctcat gcctttcctc gtcattatac
    11641 cctaaaacaa ggtgaccttt taaaagtaga tatggtttta agcgaacctt tagataaatc
    11701 aattgttgac gtttcatcgt taaactttga caacgtggct caaatgaaaa agtacacgga
    11761 aacatatagt ggtggtttgg cagactcgtg ttgggcttat gctgtaggtg aagtttcaca
    11821 agaggtgaaa gacttaatgt ctgtgacaag agaagcaatg tatattggga ttgaaaaagc
    11881 agttatcgga aaccgcattg gtgatattgg agctgctatt caggattatg cagagagtcg
    11941 tggttatgga gttgttcgtg atttggttgg tcacggtgtt ggtccaacaa tgcatgaaga
    12001 gcctatggtg cctaactatg gtacagcagg tcgaggtctc cgtcttcgtg aggggatggt
    12061 tttaaccatc gaaccaatga ttaatactgg tacttgggaa attgatacag acatgaaaac
    12121 tggttgggca cataaaacct tagatggtgg tctttcttgt caatatgaac atcaatttgt
    12181 tatcactaaa gatggtcctg ttattttaac gagtcaagga gaagaaagaa cttactaaaa
    12241 tgaaattaaa aaaattcttt gaggatttgc tggctaaact agaatataga cccattcaag
    12301 tttttatgcg tcattttcaa agtgcagaga tggacttatc agccatagca gttgcttatt
    12361 atctattagt gacagctttt ccattactag ttattgctgc taatattttt ccttattttc
    12421 atattaatgt gtcggattta ttgtcattta tgcaaaaaaa tcttccgaaa aatatttatg
    12481 aacctgcctc acgcttagca gtagatgctt tttcgaaacc gtctaccggc attttgggat
    12541 tcgcgtcatt aacagctttt tggacaatgt ctaaaagttt gacctctctt caaaaagcca
    12601 ttaataaagc atacggtgtt gaccaacaca gagactttgt gattagtcgt ttagtgggtg
    12661 tggggactgg gctgatcatc ctatttttac tgacttttgt tttaattttt tcaacctttt
    12721 caaaaccagt tttacagata attgtaaaca tgtatgattt gggagatact ctaacggcgt
    12781 ggcttttaaa tcttgcacaa ccagttacat ttttgactat atttttaggt attggaattt
    12841 tatactttat tttacctaac gcacgaatta gaaaagtacg ttatgtcatt ccagggacat
    12901 tattttcaac gtttgttatt ggttttttta gtaatttaat tagccaatac gtcttaaata
    12961 gggttgagaa aatggtagat attaaaacgt ttggttcggt cgttatcttt atcttaatgc
    13021 tatggtttat tttcttagcc cacattatga ttttaggtgc aatactgaat gccagcgttc
    13081 aagaaattgc gacaggaaaa attgaatcaa ggcgtggaga tattatgtct cttatccaaa
    13141 aatccaaaga agaaaaataa gaaaagacag ctcttgttag agttgtcttt ttttgtcttt
    13201 aaaaataaca aatttgctca agatgtagtt gagtaaaata attaatattt gagatacaag
    13261 gctttcaatg gtgttgattt tatttaaatt gtgctgaacg aattgtccga taatagaagg
    13321 aaattgatca acaaaaataa cagctaatat caaatctaat cctaatgtgc ctaatctagc
    13381 gatgacaaat gtttgtaagc gttgaagctg tccaatttta gtttgtttaa aaacaaatcg
    13441 atcatttgta aaaaaagcaa aaagtatagc tattatgtta gcaactattg cagagaatgg
    13501 agcgtcctgc gagaaattaa aaatcccttg tcgaataact aaataaagaa gggatgttaa
    13561 tagtccgaac agtaaatata agctgacttc attttgaata ctttttttca gtaacgattt
    13621 taatttaacc atatttttat tatatcacgg atgcttcaaa gtgatgagga aatctcccat
    13681 aagcttggct ttactttttg tttgtgatat acttagtaag ttgttttaca caacccttga
    13741 tgcttagttc cctttcatca agcatattac aaacgttaga agtgttaact atctaaacgt
    13801 taaaggagaa attatgaaca catttacaac tcgcgattac gctcacatgg ctattgtaac
    13861 agctatctat atcgttttaa ccataacgcc accttttaat gctattgctt atggtgctta
    13921 tcaattccgt gtttcagaaa tgctaaattt cttggccttt taccaccgaa aatacctttt
    13981 tgcggtaacg ttaggatgta tgatttctaa tctttatagt tttggaatga ttgatgtctt
    14041 tgtaggtggt ggttcaacct tactttttgt ttatcttgga accattttat tcaagcaata
    14101 tcaaaaagat tatctattta atggccttat taacaaagca ttctttttct tttctttctt
    14161 ctttgctgct tcaatgatta cggtagcagt tgaattgaaa attgtagcag gattaccttt
    14221 attattaaca tggttaacga cagctgttgg tgaattggct tcacttttgg ttggagcagt
    14281 ccttgtggat aagttgagta gacacgtcga ttttacaaaa taaatatatt ataaagcgcg
    14341 agacattgat ctcggctttt ttgtttgggt ttatagttag catagctaga aaatgctgca
    14401 acttttatga gaaatgctcc aatttttact aatatcctta gtctttgtga taaaatagaa
    14461 atatggaaaa tcgtatgaat gaattagtat ctttgttgaa tcagtacgca aaagagtatt
    14521 atacacaaga taatcccaca gtttctgaca gtcaatatga ccagttatat cgagagttag
    14581 tagaattaga aaagcaacat ccagaaaaca ttttgccaaa tagtcccaca catcgagtgg
    14641 gtggtttagt tttagaaggc tttgaaaaat accaacatga atatcctttg tattcattgc
    14701 aggatgcttt ctcaaaagaa gaattgatag cctttgataa acgtgttaaa gcagagtttc
    14761 caactgctgc ttatatggca gagctgaaaa ttgatggtct atctgtctcg ttgacttatg
    14821 taaatggtgt tttacaggtt ggggcaacac gtggtgatgg caatattgga gaaaatatta
    14881 ctgaaaattt aaagcgtgtt catgatattc ctctacattt agaccaaagt ttagatatta
    14941 ctgttcgcgg agagtgctac cttcccaaag aatcttttga agctattaac atagaaaaac
    15001 gagcaaatgg tgagcaggaa tttgctaatc ctaggaatgc agcagcagga accttgcgcc
    15061 aattaaacac aggaattgtt gcgaaacgta aattagcaac ttttctttat caagaagcta
    15121 gccctacgca aaaagaaaca caagatgatg ttttaaaaga gctagaaagc tatggctttt
    15181 cagtcaatca tcatcgttta attagctcgt ctatggagaa aatttgggat tttatccaaa
    15241 ctatagaaaa agatagagtt tccctccctt atgatatcga tggtattgtt attaaggtta
    15301 atagcttagc aatgcaagaa gagctgggct tcactgtaaa ggcacctcgt tgggcgattg
    15361 catataaatt tcctgctgaa gaaaaggaag cagaaatatt atctgttgat tggacagttg
    15421 ggcgaacagg agtcgtaaca ccaacagcta acctaactcc agttcaacta gcagggacaa
    15481 ccgttagtag ggccactctg cataatgtag attatattgc cgaaaaagat atccgtattg
    15541 gagatactgt tgttgtatac aaagcagggg atatcatccc tgcagtctta aatgtcgtta
    15601 tgagtaagcg aaaccagcaa gaagtcatgc ttattcctaa actatgtcct tcttgtggta
    15661 gtgaattagt tcattttgaa ggtgaggtgg ctttacgttg tattaatccg ctgtgcccca
    15721 accaaatcaa agaacgttta gctcattttg ctagccgaga tgctatgaat attacaggtt
    15781 ttggtccatc cttagtcgaa aaattatttg atgctcattt aatagcagat gttgcagata
    15841 tttatcgttt aagtatagag aatttattaa ctttagatgg tattaaagag aaatctgcaa
    15901 ctaagattta tcatgcaatc caatcttcaa aagaaaattc tgctgaaaaa cttctttttg
    15961 gcctaggtat tcgtcatgtg ggaagcaagg cgagccgcct tttattggag gaatttggta
    16021 atttgaggca actaagtcaa gcaagtcaag aaagtattgc ctcaattgat ggactaggag
    16081 gggttattgc caaatcactt cataccttct ttgaaaaaga agaggtagat aaactactag
    16141 aggaattaac ttcctataac gttaatttta attatctagg aaaacgagtt agtacagatg
    16201 cacaactttc aggtttaaca gtcgttttaa ccggtaagct tgaaaaaatg actcgtaatg
    16261 aagcaaaaga gaaattacaa aaccttggtg ctaaggtgac aggtagcgtt tctaaaaaga
    16321 ctgatttaat tgttgcagga agcgatgcag gaagtaaact aaccaaggct caagatttag
    16381 ggattacaat acaagatgaa gactggttac tcaaccttta agtgaggaag aaatgaagaa
    16441 acaaaaaaaa gcgcgtttaa tttataaccc aacttctggt caagaaatta tgaaaaaaaa
    16501 tgtagcggaa gttcttgata tacttgaagg atttgggtat gaaacatcag cctttcaaac
    16561 aacaccaact aaaaattctg ccagagatga agctactaga gctgctcaag ctggttttga
    16621 tttgattgtt gcagcaggtg gagatggtac tattaatgaa gttgttaatg gtattgcacc
    16681 tttgaaacgt cgtcctaaga tggcaattat tccaactgga acaactaatg attttgctcg
    16741 tgccttaaaa ataccgcgag gtaatcctat tgaagcaact aaattaattg gtaaaaatca
    16801 aatcgttaag atggacattg gtcaagctca agaggacaat tattttatta atattgcagc
    16861 tgcaggtagt ttgacagagt taacttatag tgtaccaagt caattgaaaa caacgtttgg
    16921 ctatcttgct tatctagcaa agggtgttga attattacct cgagttcgaa aagtaccagt
    16981 caaaattact cacgataagg gtgaatttat cggtgatgct tcaatgattt ttgttgccat
    17041 cacaaattct gtaggagggt ttgaacaaat tgcacctgat gccaaattgg atgatgggaa
    17101 atttaccctt attttagtaa aaactgcaaa tcttattgag attatgcatc tcattcgttt
    17161 ggtgctagca ggtggaaaac atattaatga taaacgagta gagtatatta aaacgtctta
    17221 tctaactatc gaacccctat cagacgagcg tatgatgatt aacttagatg gagaatatgg
    17281 aggagatgct cccattactc tagcaaacct taaaaatcat attaggttct ttgctaatac
    17341 tgatgaaatt tcagatgatg ctttagtttt agataaagat gaactagcga tagaagctat
    17401 tgcacagaag tttgctaatg aggtagatga tttagaagga ttaggagatt gatgaatgga
    17461 taatactgtc attgtacatt accatagccg aaagaaaaat tactttaatt taagtttatg
    17521 gcagtggcgc gatggctcag agggtcaaga tgctcatttt tcacggtttg acagtttcgg
    17581 tgctgttgcg atactaaaat atcccttgcc atatttttta agccattatt atgtgattgt
    17641 taaggataaa aattggcatc ataaaacaat agattatcgc attgattgta ctcaaggtgg
    17701 tgcacgtaaa gaagtttgga ttgtagatgg ggatgacaat ctgtattatt ctcgtcaagc
    17761 agcagttagt agccatcatt atagtagacg ccaacctcat gcctacgaca tggcaatcaa
    17821 tagtagagcg tttgacaaaa aatggggatt tgatggttgg ttaggttttg aatattcaga
    17881 aactaaaacg gcttttagat tatgggcccc tactgcggaa cgtgtagaac tgattttata
    17941 tcattcgaca gatgaaacag caagtgtatc aaaagttctt tctatgaaaa gagggacagc
    18001 cgtcaattat aaaaatcata aagaaaatac ccatggagtc tggtttactg aacttgaagg
    18061 aaactataat taccaagcat atacttatcg agtctactat agacgacgta cctttaaaat
    18121 tacgagagac ccttattcca ttgcaacgac tgcaaatggt aaaaggtcta ttgttattgc
    18181 tccagaagca ttaactccgg aagggtttaa aataagtcat ggaaaagaag ctaagtggcg
    18241 gctcgaaaac cccaatcaag cagtcattta cgaaatgcat gtgcgtgatt tttcgatatc
    18301 tgagacttca ggagttaaga cagattatca cggaaaattt aagggattgc atcaaaaagg
    18361 tacggttaat cagcatggtg ataaaacgac ctttgattat gtacaagacc taggagttaa
    18421 ctatatccaa cttcaaccaa ttttcgatca tcatcagaca tttgatgatg atggtcacta
    18481 tgcttataat tggggatatg atcctgaaaa ctacaatgtt ccagaagcaa gcttctctag
    18541 taatccacat gaacctgcaa cgcgtatttt agaattaaaa tctgctatcc aagcgtacca
    18601 tgatgcaggc attggtgtta ttatggatgt tgtctataat catacttttt catcaactga
    18661 ttctgcattt caactaacgg ttccagatta ttattatcga atgaatcaca atggaacatt
    18721 tcaaaatggg tcaggctgtg gtaatgaaac agctagtgaa aaagaaatgt gtcgtaagta
    18781 tattcttgat tcagttttgt attgggtgaa agaatataat attgatggtt ttcgatttga
    18841 tttgatggga ctacatgatg tagaaacgat gaacattata agaaatgagc taaataaaat
    18901 cgaccctcgt attttggttt atggagaagg atgggacatg ggagctggtc tcacgcctca
    18961 aaataaagct aaaaaagata atgcttatca gatgcctgga attggctttt tcaatgatga
    19021 tgtaagagac gctgtcaaag gtgcggaaat ttacggagaa tttaaaaaag gcttagtttc
    19081 tggaaacagt acagaagata ttgttgcaaa aggcatttta ggaagtgatg agttagtatc
    19141 atacattgat cctagtcaag ttttaaatta tgttgaagca catgacaact acaacttaaa
    19201 tgatttgcta tgggaattac atcctaatga caacgagaaa caacatattt accgagttga
    19261 agtagctagt gctatgaatc tattgatgca agggatggct ttcatgcaat taggtcagga
    19321 gtttcttcga acaaagtgtt atcctacagg tgataaaggt caacttactc aagcagataa
    19381 agaaagggct atgaattctt acaatgcccc agaccaagtt aatcaggtca actgggataa
    19441 tgttaccttt cataagagta ctattaattt tattaggaaa ataattactt tgaagacaaa
    19501 ctctccttac ttttcttatt ctagctttga agagattcgc aaacatgttt ttgtagagtc
    19561 tgctcaatac cattcaggtt tcataagttt tacagtcgaa gaacataaga aaataaaaat
    19621 aattttcaat attaaaggaa aacgcttgca gaattctttc ataggtgata taataatatc
    19681 aaatcatgaa gaaggaaaag cgatagattc attagatgat gagctttctg taatgatcct
    19741 tgatatcaca aaataacctg aaaacattca ggttattttt gtgtatatta aggaatttga
    19801 tttgaatttg agtttcttat attttagata atttttcgga aatatagagg gtgaaatatt
    19861 ttcagatgcg tctgtatgag gaaaatgtat tcctcttttt ttgtaccatt gaggaaatat
    19921 ttttgtctta tattgactag taggacctgt gaggtgagta gatgtccaat aaagatgagt
    19981 tatatacttt tggaataggt gaaaattttc atctccagaa ttatttaggc gtacatagcg
    20041 aaaatggaag cttttgtttc agagtttggg ctcctaatgc tgaaaatgtt caagttattg
    20101 gagattttac agattggcga aatcgacctc tccaaatgaa caaaaatcaa gcaggagttt
    20161 gggaagctaa cagtttggat gcgcgcgaag gtgatttata taaatatttg gttactcgta
    20221 aaggtggtca agtagttgag aaaatcgatc ctatggcagt ttatatggaa agacgtcctg
    20281 gaactgcaag tgttattaaa gttcttagaa ataaaaagtg ggaagatggt ctttggatgg
    20341 gaaggcgcaa acgtctgggt tttcagaagc gtccaattaa tatttacgaa gttcatgctg
    20401 gttcatggaa aaaagatgat tttggacatc ctatgacttt ttctcagtta aaagattact
    20461 taatccccta ccttgttgag atgaattata ctcatgttga atttatgccc ttaatggcac
    20521 atccgcttga tatgagttgg ggatatcaat taatggggta ttttgctttt gaacatacct
    20581 atggtactcc agaagaattt caagactttg tagaagcatg tcataaaaat aatataggag
    20641 ttcttgtaga ttgggttcca ggacacttta ttcagaatga cgatgcttta gcctatttcg
    20701 atggaactgc tacttatgag tatcaaaatc atgatcgtgc gcataactat cgatggggag
    20761 ctctgaactt tgatttagga aaaaatcaag tacaatcctt tttgatttcg agtgccttgt
    20821 tctggattga acattatcat attgatggta ttcgtgttga tgcggttagt aatatgttat
    20881 atctcgatta tgatgaagga ccatgggaag ctaaccaatt tggggataat cgtaatttgg
    20941 aaggatatca ctttttaagg aaacttaaca aagtaattaa agagcgtcat cctaatgtta
    21001 tgatgattgc tgaagagtca acagcttcta ccccaataac taaagactta gagtctggag
    21061 gattgggttt tgactttaaa tggaatatgg gctggatgaa tgatatcctg cgtttttatg
    21121 aagaagatcc tctctatagg caatatgatt ttaatttagt gacttttagt tttatgtata
    21181 tattcaatga gaactttgta ctagcttttt cacatgatga agtagtacat ggtaaaaaga
    21241 gtatgatgca taaaatgtgg ggtgaccgtt ataatcaatt tgctggttta cgtaatttat
    21301 atgcatatca aatgtgtcat cctgggaaaa aacttctctt tatgggaagt gagtttggtc
    21361 aatttttaga atggaaatat aatgatcaac tagaatggga aaacttaaat gatgatatga
    21421 accaaaagat gcaacgctat actaaacaat tgaatcaatt ttataaagat cataagtgcc
    21481 tatggcgtat tgatgattcc tttgacgggc tagaaattat tgatgcggat aataaatctg
    21541 aaactgtttt atcttttata cgaaaagatg ataaaggcga tttgttactt tgtgtcttta
    21601 atatgactcc tgtagaacgc cctaacttta ctattggtgt tccgcaagcg ggtatttatg
    21661 aagaagtttt aaatactgaa atggaagagt ttggaggtgt ttggaaaaac cacaatcctg
    21721 taacgaaaac acaagtggct acatggaaag attatgatca tactttaagt tttacattac
    21781 cagcccttgg ggcaagtgtt tggagaataa aacgtcggct tcgtaaataa gtctgcaatt
    21841 tagtaacctc attttgaaag gaaagaataa tatgaaaaat gaaatgttag cactcattct
    21901 cgccggagga caaggcactc gactcggaaa attgactcag agcattgcga aacctgctgt
    21961 tcaatttgga ggtcgttacc gtattattga ctttgcgctt tcaaattgtg cgaattcagg
    22021 tattaataat gttggagtta ttactcagta tcaaccgctg gaactcaata ctcatattgg
    22081 aaacggatct agctggggac tagatggtat cgattcggga gtaactgtat tacagccata
    22141 ttctgcaaca gaaggtaatc gctggtttca aggtaccagc cacgctatct atcaaaatat
    22201 tgactacatt gatcgtatta atccagaata tgtactcatt ctctctgggg accacatcta
    22261 taaaatgaac tatgatgaca tgctacagac gcataaagat aatttagcaa gcctaacagt
    22321 cgctgtttta gatgttcctt taaaagaagc aagccgtttt ggaattatga atactgattc
    22381 aaacgaccgt attgtcgaat ttgaagaaaa acctgaacat cctaagtcaa caaaggcttc
    22441 aatgggaata tacatttttg actggaagag attacggaca gttttaattg atggtgaaaa
    22501 gaacggcata gacatgtcag actttggtaa aaatgttata ccagcatacc ttgaatcggg
    22561 tgaacgtgtt tatacctata attttgatgg gtattggaaa gatgttggta ctattgaatc
    22621 cctttgggaa gctaatatgg aatatattgg agaagataac aaattacata gtcgtgatcg
    22681 ttcatggaaa atctattcta aaaatttgat tgcaccacct aatttcatga cagaagatgc
    22741 aaatgttaaa gattctttag ttgtagatgg atgctttgtt gcaggaaatg ttgagcactc
    22801 cattttatca actaatgtcc aggtaaaacc aaatgctatt attaaagact cctttgttat
    22861 gagtggcgcg actatcggtg aaggtgcaaa gataaatcga gctattatcg gtgaggatgc
    22921 tgttattggt gatggtgttg tgattgatgg aagtaaagaa gtagaagtta ttggttataa
    22981 agaagtagcg ggggttccaa atgaagattg ataaatatac tgcgatttta ggaaatactg
    23041 ttagttaccc agatatgcac agtttgacgg ataatcgtcc gcttgcttct ctaccttttg
    23101 atggtaagta tcgtttaatt gattttcaat tatcctcttt agctaatgca ggcgttagaa
    23161 gtatttatgg tatttttcgt gggaataatg ttcgctctgt ttttgaccat attagaagtg
    23221 gtcgtgagtg gggcttaggt acactgttga gtcactattc tttaggattc tatgatacag
    23281 acgatgatac tcgtacagtc aatggggatt attacgatca aattttaact tacttgaggc
    23341 gttcgggtag cgaccagacg atatatatga gttgtgatat gctttgtaat attgacttaa
    23401 gtcaagtaat ccacttgcat aatgcaaatg gtgcgcaagc caccgtggtt tataaaaaaa
    23461 tgccgagatc agctattgca gaggctaatc aagtcttgac cattgatgaa tctgatcatg
    23521 tagtgagtct aggtaaagct gtagataatg acgataacca aaaaatgtct gctgagattt
    23581 atgttatcga tacaccttgg ttaatcgaaa aaatggaaga ggaagctcaa aataatgaac
    23641 cacgtaaact ccgcttctta cttcgtgatt taattgttga aagtaatgct ttggcttttg
    23701 aatacactgg ttatttgtca aacatttctt ctattaaatc ctattatgat gctaacatgg
    23761 acatgttaac accaaataaa ttttattcgc tcttcttttc aaaccaaaaa gtttatacta
    23821 aggtgaaaaa tgaggaagct acttattttg ataaacaatc aaatgtctct aattcgcaac
    23881 tagcctctgg aagtattatt aaaggttatt tagaccattc tatcgtttct agaaattgtc
    23941 tccttgaaaa agggactcga gtagtcaata gtattatctt tccaaaagta aaaatcggag
    24001 aaggggcaac cattgaaaat actattattg acaaatgcgt taaggttgca tccggagtta
    24061 cattgaaagg tagtttagat aaaccgttag tgattcctaa attttcagag attaatgagg
    24121 atattataca atgaaaataa tgtttgttgc ggcagagggt gctccctttg ctaaaacagg
    24181 tggtttggga gatgtgattg gcgcactccc aaaatcactt agtaaaaaag gtcatgatgt
    24241 cgctgttgtc atgccatatt atgatatggt agaccaaaaa tttggagacc aaatagaaaa
    24301 cctcatgtat ttctatacag atgttggctg gcgtcatcaa tatgtaggag ttaagagact
    24361 ttctcaagat aatgttacgt tctactttat tgataatcaa tattattttt atagaggtca
    24421 tgtctatggt gattgggatg atggggagcg ttttgcttat ttccaattag ctgctttaga
    24481 gttgatggag aaaatcgatt ttattcccga tgtcttacac gtccatgatt atcatacagc
    24541 aatgattcct ttcttattga aagagaagta ccactggatt caggcctata ataatataag
    24601 agcagttttt actattcaca atattgaatt tcaagggcag tttggtccag aaatgttagg
    24661 agatttattt ggagttggcg ctgagagata tgaagatgga actcttagat ggaataattg
    24721 tcttaattgg atgaaagcag caattcttta ctcagataga gtgacaacgg tatcaccaag
    24781 ctacgcaaat gaaataaaaa ctccagaatt tggtaaaggt ttagatcaaa ttatgcgtat
    24841 ggaagcaggc aaattatctg gaattgtaaa tggtattgat agcgatttgt taaatccaga
    24901 aacagatgct tttttgccat atcatttttc aaaaagcaac ttagagggca aaattaaaaa
    24961 taagttggct ttacaggaaa atttgggctt acctcaagat aaaaatgtac cattaattgg
    25021 tattgtttca cgacttaccg atcaaaaagg atttgatatt attgcttctg aattggacaa
    25081 tatgcttcaa caagatattc aaatggttat tttaggaaca ggctatcacc attttgaaga
    25141 aacgttctct tattttgcta gtcgttaccc tgaaaaacta tctgctaata ttacttttga
    25201 cttaagatta gcgcaacaga tttatgcggc ttcagatatc ttcatgatgc caagtgcttt
    25261 tgaaccttgt ggtctctcgc agatgatggc tatgagatat ggtagcttgc ccttggtaca
    25321 tgaagttgga ggattaaaag atactgtggt agccttcaat caatttgatg gtagtggtac
    25381 aggtttttca tttaatcatt tttcaggcta ttggttaatg caaactctaa aactagcatt
    25441 agaagtttat aatgattatc ctgaagcgtg gaaaaagtta caatggcaag ctatgtcaaa
    25501 agatttttca tgggatacag catgtgttgc ttacgaacaa ctgtatcagc aactacaata
    25561 atcaccctat tttagtgatt agttgataag cttatcgaat ctctatgcta aacgtttctt
    25621 atgtaaaacc actttagtat agagatttta ttatcctata aggtaaaata ttattaatgt
    25681 aaagagacac tatatcaaaa ataaagtgtt gtcatatctt aataagggat taaattaaag
    25741 ctaaaaaaac taaaaattgt tatttgaaaa ggattgtgta aaccatctca aactatttca
    25801 tgaaaacgta atcaaatgag gaaattcatt gacatgcaca agaaatagga ataaaataac
    25861 ggagtaaata ttttttaaag gagaattcta ttatgaattt agctatttta gcacttggtt
    25921 tcgccgtgat gggtgtttca atcggtgaag gaattttggt tgcaaatatt gccaaatcag
    25981 cggctcgtca gcctgaaatg tttagcaaac tgcaaacatt gatgtttaca ggtgtggcct
    26041 tcattgaagg taccttcttc gttctatttg cgtttacatt cttagtaaga tagacgacaa
    26101 tttagaaaaa aaggagggat gaggattgga atctacaagt aatccaaccg tatctttttt
    26161 aggcattgat tttgatttaa ccatcctagc tatgtctctc ttgacaatta ctattatctt
    26221 tatcttagtc ttctgggcta gtcgtaagat gacaataaaa ccaaaaggta agcagaatgt
    26281 cttggaatat gtctatgagc ttgttaataa tacaatctca caaaatttag gacactatac
    26341 taaaaattat agtttattga tgtttatttt attcagtttt gtcttcattg ctaacaactt
    26401 aggattgatg acatcattga aaacacatga gcataatttt tggacatctc cgaccgctaa
    26461 ttttggtgta gatattacat tatcattatt agtagcattt atttgtcata ttgaaggtat
    26521 tcgcaaaaaa ggaattggtg gttatctcaa aggtttctta tcaccaacac cagcaatgct
    26581 tccgatgaat ttattggaag aagttactaa tgttgcttca ttagcattgc gtctttttgg
    26641 taatatcttt tctggtgaag ttgtcactgg attactttta caacttgcag ttttatcacc
    26701 atttacaggt ccactagctt ttgcattgaa tatcgtttgg acagcattct caatgtttat
    26761 tggtttcatc caagcgtatg tgttcattat cttgagctca agttatattg gacacaaagt
    26821 tcatggtgac gaagaagaat aagaaaagag aggagaaata tgtcaatact tattaatagt
    26881 acaacgattg gtgatatcat tatcgtatct ggctctgtgc ttctcttatt tatcttaatt
    26941 aagacttttg catggaaaca aatcacaggt atttttgaag cccgtgaaca aaagatagct
    27001 aatgatattg atactgctga acaggcacgt caacaagctg aagcttttgc aactaagcgc
    27061 gaggaagaac tatctaacgc taaaactgaa gctaatcaaa tcattgataa cgctaaagag
    27121 acaggtcttg caaaaggtga tcaaattatt tcagaagcaa aaactgaagc tgatcgatta
    27181 aaagaaaaag cacatcaaga tattgcccaa aataaggcag aagcactagc agatgttaag
    27241 ggtgaagtag cagatttaac agttttatta gctgaaaaaa ttatggtatc aaatcttgat
    27301 aaagaagctc agagtaatct cattgatagc tatattaaaa agctaggaga cgcctaatga
    27361 ataaaaagac gcaggctcta attgagcaat attcaaaaag ccttgttgaa gttgccattg
    27421 aacataaaat tgttgaaaaa atccaacaag aagtagcagc tttaattgat atttttgaaa
    27481 caagcgagct tgagggtgtc ctgtcaagtc ttgccgtttc acatgatgaa aaacaacatt
    27541 ttgtgaagac attgcaaaca tcatgttcaa cttatcttgt taatttcctt gaggttattg
    27601 tacaaaatga acgtgaagca cttctatatc ctattttgaa atcagttgat caggaactta
    27661 tcaaagtgaa tggtcaatat ccaattcaaa ttacaactgc agttgctttg tctccagaac
    27721 aaaaagaacg tctttttgat attgctaaaa ctaaattagc tttaccaaat ggacagttag
    27781 ttgagcacat tgacccatct attgttggtg gatttgttgt caatgcaaac aataaagtaa
    27841 ttgatgcaag tgttcgtaat caattacatc aatttaaaat gaaattaaaa tagaaagtgg
    27901 tgtgactttt ggcaattaat gcacaagaaa ttagcgcttt aattaaaaag caaattgaag
    27961 acttccagcc aaattttgac gtcacagaaa ctggtattgt tacatatatc ggtgatggta
    28021 ttgctcgtgc tcgtgggcta gataatgcta tgagtggaga acttcttgaa ttttcaaatg
    28081 gtgcgtacgg tatggctcaa aaccttgagt ctaatgacgt gggtatcatt attttgggtg
    28141 atttctcaga aattcgtgaa ggtgatgtgg taaaacgtac tggtaaaatt atggaagtac
    28201 cagtagggga agctatgatt ggacgtgtcg tcaatccgct tggtcaacca gttgatggac
    28261 taggtgaaat tgagacgaca gcaacacgcc cagttgaaac accagcacct ggtgttatgc
    28321 aacgtaaatc agtttttgag cctcttcaaa caggtctcaa agcgattgat gctttggttc
    28381 ctatcggacg tggtcaacgc gaattgatta tcggtgaccg ccaaactggt aagacgtctg
    28441 tagcgattga tgcaatcttg aatcaaaaag gtcaagatat gatctgtatc tatgtggcta
    28501 tcggtcaaaa agagtctaca gtacgtacac aagttgaaac tttgcgtaaa tacggtgccc
    28561 ttgattatac aatcgttgtg acagcttcag catcacaacc atctccatta cttttcattg
    28621 ctccatatgc aggagtagca atggctgagg aattcatgta taacgggaaa cacgttttga
    28681 ttgtttatga tgatttatca aaacaagccg tagcttatcg tgaactttcg cttcttcttc
    28741 gccgtcctcc aggtcgcgaa gcttacccag gggatgtttt ctaccttcat agtcgtcttt
    28801 tggaacgttc agctaaagta tcagatgctt taggtggagg ttcgattaca gcgcttccgt
    28861 tcattgaaac acaagcaggt gatatctcag catatattgc tacaaacgtt atttcaatta
    28921 ctgacggcca aattttcttg caagaaaatc tctttaactc aggtattcgt ccagcgattg
    28981 atgcaggttc atcagtatct cgtgttggtg gtgcagcgca aattaaagcg atgaagagag
    29041 ttgcaggtac gcttcgtcta gatcttgctt cttatcgcga acttgaggca ttcactcaat
    29101 ttggttcaga ccttgatgca gcaacacaag caaaactaaa tcgtggtcgt cgcacagtgg
    29161 aagtgttaaa acaaccatta cacaaaccat taccagttga gaaacaagtt gtgattttat
    29221 atgctctaac acatggattc cttgatgatg tacctgtaaa tgatatttta gcttttgaag
    29281 aagctttgta tgattacttt gatgctcatt atgataatct cttcgaaaca atccgcacaa
    29341 caaaagattt accagaagaa gcagaattag atgctgctat tcaagcattt aaagatcaat
    29401 cacaatttaa ataataggag ggttctaata tggcaggctc tcttagtgaa attaaagaca
    29461 agattctctc tacagaaaaa acaagtaaga ttactagtgc gatgcagatg gtctcatctg
    29521 ctaagttggt taaatctgaa caagctgcta gagattttca agtgtatgcc tctaaaattc
    29581 gtcaaattac tactaacctc ttaaagtcag acttagtatc tggatcagat aaccctatgt
    29641 tatcttcacg tccggtgaag aaaacaggtt atattgttat tacttctgat aagggtttag
    29701 ttggtggtta caattcaaaa atcttaaaag cgatgatgga cactataact gactatcata
    29761 ctgagaatga tgattatgcc atcatttcaa ttggtagtgt tggttctgat tttttcaaag
    29821 cacgtggaat gaatgtttct ttcgaattac gtggtttaga ggaccaacca agttttgatc
    29881 aagttggtaa aattattgct caagcagttg aaatgtacaa aaatgagttg ttcgatgaat
    29941 tatatgtttg ctacaatcac catgttaaca gtttaactag ccaagtcaga atgcaacaaa
    30001 tgcttcctat caaagaattg gatgcagaag aggcttctga ggatagagtg attactggtt
    30061 ttgaacttga gccaaaccgt gaagttatat tggagcaatt attgcctcaa tatactgaga
    30121 gtttgattta tggagctatt attgatgcta aaacagcaga gcatgcggct ggtatgacag
    30181 ctatgcagac tgcaactgat aatgctaaaa atgttatcaa tgatttaact attcaatata
    30241 accgcgctcg tcaagcagct atcacacagg aaattacaga aattgttgca ggtgctaatg
    30301 cacttgaata attaattagt ctatagaaaa aatagtgaac aaaggtattt taaatccttt
    30361 gtatcatatg ataaggagaa atacatgagc tcaggcaaaa ttgctcaggt agttggacct
    30421 gtcgttgacg ttgtatttgc aagtggcgac aaacttcctg agattaataa tgcattgatt
    30481 gtctataaaa atggcgataa gtcacaaaaa gtagtacttg aagttgctct tgaacttggt
    30541 gacggcctcg ttcgtacaat cgctatggaa tcaactgatg ggcttacacg tggtttggaa
    30601 gtattagata ctggtcgcgc aattagtgtg ccggttggta aggatacttt gggtcgtgtc
    30661 ttcaacgttc ttggagatgc tattgacctt gaagagcctt ttgcagaaga tgcagaacgt
    30721 caaccaatcc ataaaaaagc accatcgttt gatgaattat caacatcatc agaaatctta
    30781 gaaactggta ttaaagttat tgacttatta gcaccttact taaaaggtgg taaagttgga
    30841 cttttcggtg gtgcaggtgt tggtaaaacc gttcttattc aagaattaat ccacaacatc
    30901 gcccaagaac atggtggtat ttcagtattt actggtgtag gagaacgtac tcgtgaaggg
    30961 aatgaccttt attgggaaat gaaagaatct ggcgttattg aaaaaacggc tatggtcttt
    31021 ggtcaaatga atgaaccacc aggagcacgt atgcgtgtgg cacttactgg tcttacaata
    31081 gctgagtact tccgtgatgt agaaggacaa gatgtgcttc tcttcattga taatatcttc
    31141 cgtttcacac aagctgggtc agaagtgtca gcgcttttag gtcgtatgcc ttcagccgtt
    31201 ggttatcaac caacacttgc tacagaaatg ggacaattac aagagcgtat cacttcaact
    31261 aaaaaaggtt ctgttacatc aatccaagct atttatgtgc cagcagatga ctatactgac
    31321 cctgcgccag ctacagcctt tgctcactta gattcaacaa ctaaccttga acgtaaattg
    31381 acacaaatgg ggatttatcc agccgttgac ccattagctt caagttctcg tgcactcaca
    31441 ccagaaatcg ttggagatga gcactatgaa gttgcaacag aagtacagcg tgttcttcaa
    31501 cgttaccgtg agttacaaga tatcattgcg attttaggta tggatgaatt atcagatgag
    31561 gaaaaaacgt tagttggacg tgcacgtcgt atccaattct tcctttctca aaacttcaat
    31621 gttgcagaaa catttactgg tcaaccaggt tcatatgttc cggttgaaga aactgttcgc
    31681 ggattcaaag aaatcctaga tggtaaacat gatcaaattc cagaagatgc cttccgtatg
    31741 gttggtggta ttgaggatgt tatcgcaaaa gcagaaaaaa tgaactatta atgaggtgat
    31801 gctatggcac aattgactgt tcaagtagta acaccagatg gtattagata tgaccatcac
    31861 gctagtctca ttactgttag aacgccagat ggtgaaatgg gaattttacc aggtcacata
    31921 aatttgattg ctccccttaa tgttcatcag atgaagatta atcgttctca tcaagaaggt
    31981 gtagactggg tagctgttaa tggtggtata attgaagtta atgaggatca ggtgacaatt
    32041 gttgcggatt cagcagaacg agcacgtgat atagacctta accgtgcaga acgggctaag
    32101 gaacgcgctg agcgtgcatt agaaaaagct caaacaactc agaatattga tgagatgcgt
    32161 agagctgagg ttgctcttcg tcgcgcaatt aaccgtatta gcgtcggtaa aaagtagaaa
    32221 aatggtctaa ttagttttgt gactaattag gcttttttag tgtataatag aaggcatgaa
    32281 tatgatatta aatatagtaa ctatagttag taattttatt tttattgccc ttttttatca
    32341 actttttgta gatttatttg actggtctaa aatgattaaa atgtcccctc aaaacatctc
    32401 aaaattaaaa gtttttattt tgttgataag tatagttggt ggctatgtgg ttagtcattt
    32461 tttattggaa gtgattcagt tgtgtcaatc aatcttttgg gctctccaat aaaggaaata
    32521 ttagctgaac tatgatataa tcaacttact attgttataa gaatggagat tcaaatggat
    32581 aaaattattg tggaaggtgg gcaaacgcag cttcaaggtc aagttgttat tgaaggagct
    32641 aaaaatgcag ttttacctct tttagcagcc acaattttac caagccaagg gaaaacattg
    32701 ttgaccaatg ttcctatctt gtctgatgta tttacaatga acaatgttgt tcgtggttta
    32761 gatattcaag ttgactttaa ttgcgataaa aaagaaatct tagtggatgc ctcaggtgat
    32821 attttagatg ttgcacctta tgaatttgtt agtcaaatga gagcttctat tgttgttttg
    32881 ggacctattt tagctagaaa tggccatgct aaggtttcaa tgccaggcgg ttgtacaatt
    32941 ggtagccgac caattgacct tcatcttaaa ggtttagaag ctatgggagc tacgattaca
    33001 caaaatggtg gtgatattac tgcccaagct gaaaagctta agggagctaa tatatacatg
    33061 gattttccct cagtcggtgc cactcaaaac ttaatgatgg ctgctacact tgcttcaggc
    33121 acaacaacga tagagaatgc tgcacgtgaa ccagaaattg tagaccttgc gcagttacta
    33181 aataaaatgg gagctaaagt aaagggtgct ggtacggaaa cattaacgat cattggtgtt
    33241 gatgctttac atggtacaga acatgatgtt gtccaagatc gtattgaagc tggaaccttt
    33301 atggtggcag ctgctatgac ttctggaaat gttctagtaa aggatgctat ttgggaacat
    33361 aaccgtcctt taatctctaa attgatggag atgggtgttg aagtgtcaga ggaagaagat
    33421 ggtattcgtg taaaagctga tactaaaaaa ttgaagccag tgactgtgaa aacactccct
    33481 catccaggat ttcctactga tatgcaagct caatttacag ctctaatggc agtagttaat
    33541 ggcgaatcaa ctatgattga gacagttttt gaaaatcgtt ttcaacattt ggaagaaatg
    33601 cgtcgcatgg gattacaaac ggaaatttta cgagatactg caatgattca tggtgggaga
    33661 gctttacaag gtgcaccggt catgtctact gacttacgag ctagtgcagc tttaatcttg
    33721 gcaggaatgg ttgcgcaagg gcaaacagta gttggccaat taacccattt agaccgtggt
    33781 tattatcaat tccatgaaaa attagcggct cttggtgcta atattaaacg tgtaagtgag
    33841 gcgtaatatg gcatctagtg gttggaaata tgtattaaaa caaatcggtc taatagtatt
    33901 agtcatacta ttagccttgc tgtttttagc ggttggattg atgttgggtt atagtgtttt
    33961 tggtgacgga gagcatgctt actctatttt atcgctggat aaatggcaaa atattattgg
    34021 aaaattttta ggaaaataga aagagccact ataagttatt tgatgcttat agtggtttcc
    34081 tttgagagtt aattttagca gtagaataat acaatgacaa aaaaacaaaa acaaataaca
    34141 gcttcgattg taactttgct tttttcatta ctggcaatag ggttaggtga aagcaaatct
    34201 ttgcctaatg atcacatttt aaaacaggtg acagatttaa ttattagtaa tgaatctggc
    34261 aaaaatactt cccttaaaaa tgtatcagga actcctagta gagagttatc tgaatctgtt
    34321 ttgactagca atgttaaaaa acaattggga acaaatatag cttggaacca gtcaggtgct
    34381 tttatcatta atcaaaataa aacagatttg aatgctaaag tgtcaagtgc accatatgct
    34441 attaatgaaa taaagaaggt taataaccaa attgttccga ctaaagcaaa tgccttatta
    34501 acaaaagcaa ctcgtcaata tcgaaatcga gaagaaacag ggaatggtag aacttattgg
    34561 aaacccgctg gatggcatca gataaatgga ttaaaaggga gctataacca cgctgtggac
    34621 agaggccatt taattggtta tgctttagta gggagtttaa gaggatttga tgcctccaca
    34681 agtaatccta aaaatattgc cacacaagca gcttgggcaa accaagctaa tagcaaccaa
    34741 tcaacagggc aaaattatta tgaaacttta gttcgtaaag cattggatcg tcataaaact
    34801 gttaggtatc gtgtgacttt aatttatgac agagataatc ttttatcttc gggatcacat
    34861 attgaggcta aatcttcaga tggaagttta gaatttaacg tttttattcc taatgtacaa
    34921 tcgggattat tatttgatta tgcaacagga aaagtgaagc agacaaagta atctttatct
    34981 aatagtgctt gttaaagact gaaaaatttg ataaaatagt ctttaattga atagaggtct
    35041 gtgagactag taaaccaagg gaagctgctt agggagttca gccgtgactg caagctgaat
    35101 cagtgaaaga gggtggaatt cactcacaaa taggactata attaaggtct gattggtcga
    35161 tcagatagaa aacggatggt accgcgtgtc aacgctccga ttaaggggta gtggcacgct
    35221 tttttttgtt ttcaaagcta cttattttga cttggaggaa agatggattt acaaaaacaa
    35281 ttagaagagt tgaaaatatc aactcaagaa aagcttaaag aaatgactgg gaaccacact
    35341 aaagaattac aagacttacg tgtacaagtt ttaggaaaaa aaggttctct aacagaatta
    35401 ctaaaaggtc tgaaagattt atctaatgat ttacgaccag ttgttggaaa acaagtcaat
    35461 gaagtacgtg atattttaac aaaagcgttt gaggaacaag ctaaagtagt ggaagctgcg
    35521 aagattcaag cacagttaga gtctgagagt gtcgacgtta ccttgcctgg tcgtcaaatg
    35581 acattaggtc accgtcatgt cttaacacaa actagcgaag agattgaaga tatcttttta
    35641 ggtatgggat tccaagtggt tgatggtttt gaagttgaga aagactacta taattttgaa
    35701 cgaatgaatt taccaaaaga tcatccagcc cgtgatatgc aagatacttt ttatattaca
    35761 gaagaaatct tgctacgtac tcatacttcg cctgttcaag cacgaacaat ggaccaacat
    35821 gatttttcta aagggccttt aaaaatgatt tctcctggac gtgttttccg tcgtgatact
    35881 gatgatgcta ctcatagcca tcaattccat caaattgaag gcttagttgt tggagaaaat
    35941 atttcaatgg gcgatctcaa aggaacactt caactgattt ctcaaaaaat gtttggagca
    36001 gagcgtaaaa tccgacttcg tccttcatat ttcccattca cagaaccttc agttgaagtt
    36061 gatgtctctt gcttcaagtg cggaggtaag ggatgtaatg tctgcaaaca gacaggttgg
    36121 attgagatat tgggtgctgg tatggttcac ccaagtgttt tggaaatgtc tggtattgat
    36181 tctgagaaat actccggttt tgcctttggt ttaggtcaag aacgtattgc aatgttacgc
    36241 tacggtatca atgacattcg tggcttctat caaggtgatg ttcgcttcac agatcaattt
    36301 taagatttta gatagcaagt agcatcattg gtgtgatgat aaatttatgt taacgctatt
    36361 accttactca ataggaggtt ttctaatgtc tcaaattact tttaaaaagg ttggccttga
    36421 taatgtgaat attttacaga atattgctat agaaaccttt cggcagacgt tttctcatga
    36481 taatagtgag gaacaacttc aagcattttt caatgagtct tacactttac cagtattaaa
    36541 atcggaaatt acccatgctg aatctgatac ttattttgtt tatcttgata cagatttagt
    36601 gggttattta aaagtaaact ggggaagtca gcaaactgaa aaagatcttg ataaagcatt
    36661 tgaaattcaa aggatttatc ttctagatgc ctaccaaggg caaggtatag gtaaggctac
    36721 tttcgaattt gctttggatt tagcttataa atcaggctta gattgggctt ggttaggtgt
    36781 ttgggagttt aatcataaag ctcaggcatt ttacgctaaa tacggatttg aaaaattttc
    36841 tgagcaccaa ttttcagtag gtgataaagt ggatacggat tggttattaa gaaaaagttt
    36901 acactaaaaa ctacaaagca cttgtataaa aataaaagaa aataaataga gagaaattac
    36961 atgttagtat cgtataaatg gttaaaagag ttagtggatg ttgatgttac aacagcagaa
    37021 ctagctgaga aaatgtcaac cactggtatt gaggttgaag gtgtggagac accagcggaa
    37081 ggtttatcaa aattagttgt aggtcatatt gtttcgtgtg aagatgttcc agatacccac
    37141 ttacatttgt gtcaagttga tacaggggat gatgaacttc gtcaagttgt ctgtggtgct
    37201 ccaaatgtca agactggtat taatgttatc gtagcggttc ctggtgctcg tattgcagat
    37261 aactataaga ttaaaaaagg taaaatccgt ggtatggagt cacttggaat gatttgttca
    37321 cttcaagagt tgggcttatc ggaaagtatc attccaaaag agttttctga tggtatccaa
    37381 attttacctg agggagctat acctggtgat agtattttct cttatcttga cttagatgat
    37441 gaaattattg aattatcaat tacaccaaac cgtgcagatg ctctttcaat gcgtggtgtt
    37501 gcccatgagg tagcagcaat ttatgggaaa aaagttcatt ttgaagaaaa aaatctcatt
    37561 gaagaagcag aaagagcagc tgataagatt tctgttgtca ttgaatcaga taaagtttta
    37621 tcttactcgg ctcgtattgt taagaatgta acagttgcac caagtccaca atggcttcaa
    37681 aataaactga tgaatgctgg tatacgtcca attaataatg ttgttgatgt gacaaactat
    37741 gttcttttga cttatggtca accaatgcat gcttttgatt ttgataaatt tgatgggaca
    37801 actatagtag cacgtaatgc tgagaatggg gaaaagttaa ttactcttga tggtgaagag
    37861 cgtgacttaa tagctgatga cttggtcatt gcggtaaatg atcaaccagt tgctttggca
    37921 ggcgttatgg gaggacaatc aactgaaatt ggtagttctt ctaagacagt ggtccttgaa
    37981 gcggcagtat ttaatggaac aagtatccgc aaaacaagtg gaagactcaa ccttcgttca
    38041 gaatcatctt ctcgctttga aaaaggtatc aattatgata cagtttcaga ggctatggat
    38101 tttgcggctg caatgttaca agaattggca ggtggtcaag ttctttctgg tcaagttaca
    38161 gaaggagttc ttccaactga gccggtagag gtatcaacga ctcttggtta cgttaatact
    38221 cgtttgggaa cagaacttac ctatactgat attgaagaag tatttgaaaa actcggcttt
    38281 gctatttctg gttcagaagt gaaatttact gtgttggtac cgcgtcgtag atgggatatt
    38341 gccattcaag ctgacttagt tgaagagatt gcccgtattt atggttacga aaaattacca
    38401 acaacattgc cagaagccgg agcgactgca ggagaattaa cgagtatgca acgcttacgt
    38461 cgtagagtcc gtacggttgc tgaaggagca ggattatctg aaattatcac ctatgcttta
    38521 acaactccag aaaaagcagt gcagttctca actcaagcaa ctaatataac tgaacttatg
    38581 tggccgatga ctgttgatcg ctcagcactt cgccaaaatg ttgtttcagg aatgcttgat
    38641 acaattgctt ataacgttgc tcgcaaaaac agtaaccttg cagtttatga gataggaaaa
    38701 gtttttgaac aaactggtaa tccaaaagaa gatttaccaa ctgaagtaga gacgtttact
    38761 tttgccttaa caggtctagt agaggaaaaa gatttccaaa caaaagccaa gccagttgat
    38821 ttcttctacg ccaaaggtat tgtagaggcg ctctttatta aattaaaatt agatgttact
    38881 tttgtagcac aaaaaggttt agcaagtatg cacccaggtc gtacagcaac tatcttatta
    38941 gatgggaaag agattggttt tgttggtcaa gtacacccac aaactgcaaa acaatatgat
    39001 attcctgaaa cttatgttgc tgaaatcaat ctatcaacta ttgaaagtca aatgaaccaa
    39061 gcgttgattt ttgaagatat tacgaagtat ccgtctgtat ctcgcgatat tgcattatta
    39121 cttgcagaat ctgttagtca tcatgatatt gtgtcagcaa ttgagacatc aggggtaaaa
    39181 cgtctaacag cgattaaact gtttgatgtc tacgctggaa ataatattgc agaagggtat
    39241 aaatcaatgg cttatagctt aactttccaa aaccctaatg acaatctaac ggacgaagaa
    39301 gtggctaaat atatggaaaa aatcactaaa tcactcgttg agaaagttaa tgcagaaata
    39361 agataattta tattttacgt tgaaaaataa ataaaaaaca gcatcaatta agatgctgtt
    39421 tttttattta aaatttatag atgttctacg gagaaagtat caccgtgttg gatgtcacca
    39481 tattgaaagc ctttgttaaa ccaacgttgg cgttgttcag ctgttccatg ggtaaagcta
    39541 tcaggcacta attttccgta ggtttctttc tgaagggtat cgtctccgac ggcgtgggca
    39601 gcattcatgg cctcttcaaa gtctccttgt tctaagagat tttttcccct gatgtagtga
    39661 gcccataccc ctgcataata atctgcttga agttctagcc gaacatttaa agcatttgct
    39721 tctttcttag taagtccgtg tcgcattcta ttatacttat ccataatgcc taactctgtt
    39781 tgaatgtggt gaccaacttc gtgggcgatg acgtaggcca tagcaaaatc accagtagca
    39841 ccatatttat gtgataattc attgtaaaaa gaaatatcaa gatagatttt tttatctgct
    39901 gaacaataaa atggtcctga agcagattca cctataccac aacctgtttg aattgaattg
    39961 gtgtaaagaa caagttttgg ttccttataa tttccaaaac cttgggtttg gaattcttgt
    40021 gaccagaaat cctcagttga gccaaggact ttattaacga aatcgatttg ttctctcgtt
    40081 gcgctattat caacagaacg tgagacattc tgagattggt aactagaagg tgaggatgag
    40141 tcattaaaaa tgctggttag tccccctccg ccaagtagca gtaagatgat taaaaccaca
    40201 agcttggttt tccaactccc tcgcagcaat aaaagttgaa gaatcggtaa tccacttcct
    40261 ccgctagaga atgaacctcc gctactggag cgacgatctt caacattgtc gctttttctt
    40321 aggtcatcta ttttcataag tatctccaaa gtgttttata ggctcattat aaactatttt
    40381 aataagaaat gcttgaattt ttaactaaat atcttcccct agatttgaag ctatttttgg
    40441 tataatatta ataaactgta aaggataagt tgaagaaggt ttacttctca gttgacttat
    40501 aagataggag attcatgaaa ttactttata ctgatattaa tcatgatatg acagaaatct
    40561 tagtcaatca ggccgcgcat gctgctgaag ctggatggcg tattttttat attgctccaa
    40621 actctttatc ttttgaaaaa gagcgtgcag tattagagaa ccttcctcaa gaagcctctt
    40681 ttgctattac aattactcgc tttgctcaat tggcacgtta ttttacttta aaccagccta
    40741 atcaaaaaga aagtttaaac gatattggat tagcaatgat cttttatcgt gctttagcaa
    40801 gttttgaaga tggacaatta aaagtttttg gacgattgaa gcaagatgct tcctttatca
    40861 gtcaattggt agacctatac aaagaattgc agacagcaaa tctgagtatt ttagagttaa
    40921 aatatttaca ttctcctgaa aaatttgaag atttattagc tatttttcta gtggtttcag
    40981 atcttttaag agaaggagaa tacgacaatc aatctaaaat tgcctttttt acagaacaag
    41041 taaggtcagg ccagctagat gtggatttaa agaacactat ccttatagtc gatggtttta
    41101 cccgtttctc agctgaagaa gaagctttaa ttaaaagtct gagttcacgt tgtcaagaga
    41161 ttatcatcgg agcttatgct agccaaaaag cgtataaggc taactttact aatggaaata
    41221 tttattctgc aggggttgat tttttaagat atctagcaac gacatttcaa acaaaaccag
    41281 aatttatctt gtccaagtgg gaaagtaaat ctggttttga aatgatttcg aaaaatatcg
    41341 aaggtaaaca tgattttaca aatagtagtc acattttaga cgacactgct aaagattgta
    41401 taacaatttg ggagtgcatt aatcaaaaag atgaagtgga acatgtggct agagctatac
    41461 gtcagaaact ttatcaagga tatcgctaca aagatatttt agttttacta ggagatgtag
    41521 atagttataa actacaatta agtaagattt ttgagcagta tgatattcct tattatttcg
    41581 gtaaagctga aacaatggct gcgcaccctt tagttcattt tatggattcc ttgtctcgaa
    41641 taaaacgtta tcgatttaga gctgaagatg ttttaaatct ttttaagaca ggtatttatg
    41701 gagagattag ccaagatgat ttagattatt ttgaggctta tatctcatat gcagacatta
    41761 aaggtcctaa gaagttcttt acagattttg ttgtgggggc taaaaaattt gatttagggc
    41821 gtcttaacac tattcgtcag tccctcctta ctccactgga aagttttgtg aaaacgaaaa
    41881 aacaagacgg tataaaaaca ctcaaccaat tcatgttttt ccttactcag gtaggtttga
    41941 gtgacaattt atcgcgatta gttggtcaaa tgtctgaaaa tgaacaagaa aaacatcaag
    42001 aagtttggaa aacatttaca gatattttag agcagtttca aacaatattt ggtcaagaaa
    42061 aattaaattt agacgaattt ttatcgttat taaattcagg tatgatgcaa gccgagtatc
    42121 gtatggtccc tgctactgtt gatgtggtta ctgttaagtc ttacgatttg gtagaaccac
    42181 atagtaatca atttgtctat gccctaggca tgacacaatc acattttcca aagattgctc
    42241 aaaacaaatc tttaatatct gatattgaac gtcagcttat aaatgatgcc aatgacactg
    42301 atggtcattt tgacattatg acgcaagaaa atttgaagaa aaaccatttt gctgccctct
    42361 ctctttttaa tgctgctaaa caagaattag tgttgactat accacaatta ttgaatgagt
    42421 ctgaagatca gatgtcacca tatcttgtcg aactaagaga tataggtgtt ccttttaatc
    42481 ataaaggacg ccaatcgtta aaagaagaag ctgataatat tgggaattac aaagctttat
    42541 tatcgcgtgt tgttgacttg tatcgctctg ctattgacaa agagatgact aaagaagaac
    42601 aaactttttg gtcagttgct gttcgttatt tgcgtcgtca attaacttct aaagggattg
    42661 agataccaat aattacagat agtcttgata ctgtgacagt ttcatcagat gtgatgacca
    42721 gacgcttccc agaagatgac ccacttaaat tatcctcatc tgctttgaca actttttata
    42781 ataatcaata taagtatttt ttacaatatg tgttaggttt ggaagaacaa gactcaattc
    42841 atccagatat gcggcaccat ggaacgtacc ttcatcgtgt ttttgaaatt ctgatgaaaa
    42901 accaagggat agaatctttt gaagagaaac tcaactctgc aattaataaa accaatcaag
    42961 aagatgtttt caaatccttg tattcagaag atgcagaaag tcgttattct ttagaaattt
    43021 tagaagatat cgcgcgtgca actgcaacca tattacgaca agatagtcag atgactgttg
    43081 aaagtgaaga agaacgattt gaactaatga ttgataatac tataaaaatt aacggtatta
    43141 ttgaccgtat cgatcgttta tctgatggtt ctttgggtgt cgtggactat aaatcaagcg
    43201 ctcaaaaatt cgatatccaa aaattttata acggtctaag tccacaatta gtcacctaca
    43261 ttgatgctat cagtcgtgac aaggaagttg aacaaaaacc accgattttt ggagctatgt
    43321 acttacatat gcaggaacct agacaagact tgtctaaaat taaaaattta gatgatttag
    43381 taacaaaaaa tcatcaagct ttaacttata aaggattatt ttcagaagcc gaaaaagaat
    43441 tcttagcaaa tgggaaatac catctcaaag attcccttta ttcagagact gaaattgcta
    43501 ttttacaagc acataaccaa tcgctttaca aaaaagcatc tgagactatt aagtcaggta
    43561 agtttttgat caatccatat acagaagatg ctaaaacagt tgacggagat cagtttaaga
    43621 gtattacggg attcgaggcg gataggcata tggcacgtgc cagagcgctt tataaattac
    43681 ctgctaagga aaaacgtcaa ggtttcttaa cattaatgca gcaagaggag gaaaatgatg
    43741 acctttaagc ctttcttaaa tccagaagat attgctgtta ttcagacaga agaaaaaaat
    43801 tctgataaaa aacaaaagcg aacgcctgaa caaattgaag ccatttatac ttttggtaac
    43861 aatgtcctgg tatcagcttc ggctggttct ggaaagactt ttgtaatggt tgagcggatt
    43921 ttagataaac ttttaagagg ggttccgatt gactctttgt ttatctcaac atttacagta
    43981 aaagcggcag gtgagctcaa agaacgttta gaaaagaaaa taaacgagtc tttgaaatcc
    44041 gcagaatcag atgatttaaa acagtttctc acccagcaac ttgtcggcat tcaaacagct
    44101 gatattggta ctatggatgc ttttacgcaa aaaatagtta accaatatgg ttatactttg
    44161 ggcatatcac ctatatttag gattttacaa gataaaaatg agcaagacgt cataaagaat
    44221 gaggtctatg ctgatttatt ttctgactac atgactggga agaatgcagc ttcattcata
    44281 aaattagtca aaaatttctc aggtaatcgt aaagatagca aagcttttcg agaaatggtt
    44341 tataaagttt atgcttttag ccagtccacc gataacccca agcgctggat gcaaacagtt
    44401 tttctaaaag gtgcacaaac ttacactgat tttgaagcta ttccagatca agaagtttca
    44461 agccttttaa atgtaatgca aacaactgct aatcaattac gtgatttaac ggatcaagaa
    44521 gattataagc aattaactgc taaaggggtt ccgacagcaa attacaaaaa acaccttaaa
    44581 ataattgaaa atttagtcca ttggtctcaa gactttaatt tgctgtatgg aaaaaaagga
    44641 ttgactaatt tggcacgtga tatcactaat gtgattcctt caggcaatga tgttacagtt
    44701 gctggcgtta aatatcctat ctttaaacaa ttgcataatc gtatcgttgg acttaagcac
    44761 ttagaagtta tttttaagta tcaaggagaa tctcttttct tgttagaatt attgcaatcc
    44821 tttgttttag atttctcaga acaatattta caggagaaga ttcaagaaaa tgcttttgaa
    44881 ttttctgata ttgcccattt tgcaattcaa atattggaag aaaatcatga tattaggcaa
    44941 ctttatcaag ataagtatca tgaagtaatg gttgatgagt atcaggacaa taaccatacc
    45001 caagagagaa tgttagaatt attgtctaat ggacataatc gtttcatggt tggagacata
    45061 aaacaatcaa tttaccgttt tcgtcaggct gatccacaaa tttttaatga taaatacaaa
    45121 gcttaccaag acaatccaag tcaagggaaa ttaattattc tgaaagagaa ttttcggagt
    45181 caatcagagg ttttagacag tacaaatagt gtgtttacac atcttatgga tgaagaagta
    45241 ggagacattc tttatgatga aagtcaccaa ttaaaagctg ctagtccaag acagcaagaa
    45301 agacatccga ataataaaac acaagtttta ctattagata ctgatgaaga tgatatagat
    45361 gacagtgata gtcaacaata tgatattagc ccagctgaag caaagttagt cgcaaaagaa
    45421 attattcgat tgcataaaga agagaatgtt ccctttcaag atatcaccct cttagtttct
    45481 tcaaggacaa ggaatgatgg tatcttacaa acttttgacc gttatggtat tcctttggta
    45541 actgacggtg gggagcaaaa ttacttaaaa tcagtcgaag tgatggtaat gcttgatact
    45601 ttgcggtcaa ttgataatcc cttaaatgat tatgctctag ttgctctttt gagatctccc
    45661 atgtttggct ttaatgaaga tgatttaaca agaattgcta ttcaggatgt caaaatggct
    45721 ttttatcata aagtgaaatt gtcttatcat aaagaaggtc atcatagtga tttaattact
    45781 ccagaattat cttctaaaat agatcacttt atgaaaacat tccagacttg gcgtgatttt
    45841 gctaaatggc attcgcttta tgatttaata tggaaaatat ataatgaccg tttttattat
    45901 gattatgtag gagctctgcc taaagctgag caacgtcaag ccaatctata tgccttggca
    45961 cttcgagcaa atcagtttga aaaaacaggt ttcaagggtt tatcacgctt tattcgaatg
    46021 attgataaag ttttagaaaa tgaaaatgat ttggcagatg ttgaggtggc attaccgcaa
    46081 aatgctgtta acctaatgac tatacacaaa tcaaaggggc ttgagtttaa gtatgtattt
    46141 attttaaata ttgataagaa attttcaatg gtagacataa cttcccctct tattttaagc
    46201 cgaaatcaag gaattggtat taaatatgtt gctgatatgc gacatgaact cgaagaagag
    46261 atactgcctg cagtaaaggt tagcatggaa acacttcctt accaacttaa taaaagagag
    46321 ttacgcttag ctactttatc agagcaaatg cgtttacttt acgtagctat gacacgtgca
    46381 gagaaaaaat tatatctggt tggtaaggct agtcaaacca aatgggctga tcattatgac
    46441 ttagtatctg aaaataatca tctcccatta gctagtcgtg aaacttttgt gacctttcaa
    46501 gattggttac tggctgttca tgaaacctat aagaaacaag agttgtttta tgacatcaac
    46561 ttcgtatcat tagaggaatt aacagatcac cacataggga tggttaaccc tagtctacct
    46621 ttcaatcccg ataataaggt agagaatcgt cagtctgaag atatcgtgcg tgcgattagc
    46681 gtgttagaat ctgttgagca gattaatcaa acttataaag cggctattga gttaccgaca
    46741 gtgagaacac caagtcaagt caaaaaattc tatgagccta ttctagatat agaaggtgtt
    46801 gatgtgatgg aaactattac taaaacatca gtagatttta agttacctga tttttctaca
    46861 agtaaaaagc aagacccagc agctttagga tctgctgtac atgaattaat gcaacgcata
    46921 gagatgtcat ctcatgtcaa aatggaagat attcaaaaag ctttgacaga agtgaatgct
    46981 gaaacaagtg tcaaagcggc tattcagatt gaaaaaatca actatttttt ccaagaaacc
    47041 tcactcggta agtatataca agaagaagta gaacatctgc atcgagaggc accttttgcc
    47101 atgttaaaag aagatccgga gagtggtgag aaattcgttg tacgtggtat tattgatggg
    47161 tatctactat tagaaaatcg gataattcta tttgattata agactgataa atttgtcaat
    47221 cctctagaat taaaagaacg ctatcaggga caaatggctt tatatgcaga agctttaaaa
    47281 aaatcttacg agatagaaaa gatagacaaa tacttaattc ttttaggtgg taaacagcta
    47341 gaagtggtaa aaatggatta aagaaaggaa aaaatgatag tcgaacaaaa atttggaaat
    47401 ggttttactt ggattaacat tgaagcagag caattgcgaa cagaaacatc ggaaattcaa
    47461 gcaaaatatt tggatagtga gattattaca tatgctctgg atgattatga acgagccttt
    47521 atggaatgtt ctcatattaa aggaaaagaa gtcttgacga ttatttttaa tactattgat
    47581 ctaaagcaga aagaaagtta ttatgagacc gttccgatga ccttttgctt atcacacgac
    47641 cgtttgataa cggtgacacg gtctcgaaat agttatatgc tagaactatt gcaaaaatat
    47701 ctagaccgta atccagatgt ctctcctaaa aaatttttat ttgcagcttt aactttgatt
    47761 acaaaacaat attttaatgt tgtatcgaaa attgatagag aaaaggatat tctcaaccgt
    47821 caattgagag aacaaacaac caataagcgc ttattagcca tgagtgacct agagacagga
    47881 tccgtttatc ttttgacagc tgccaatcaa aatgcattgg tattagagca attagatgtt
    47941 catcctagtc agcgatttaa ttctgaagtg gaaaaagaac agttatcaga tgctctcatt
    48001 gaagcgcatc agttagtcag tatgacacaa ttaaacagtc aggtactttc acaattatca
    48061 tctactttta ataatgtctt aaataataac ttgaacgaaa acttaacagg tttgaatatc
    48121 atttcgatta atttagcgat tattgcagca attactggct tttttgggat gaatattcca
    48181 ttacctctta cagaatcacg gagttcatgg ttaattgtta ttgctacttc ggtcttactt
    48241 tgggttatta ttgctcaaat tttaaaacga gtttttaact taagaaaata aaaatattcg
    48301 gtgcttacgc accgaatatt tttttattta cctaagcaga attggctgaa gagctgagtt
    48361 attagttcat ctggagctgc atccccagtg atttctccaa gtatttccca agttctagtc
    48421 atatcgactt gtaagagatc aacaggcatt cctaattcca agccctcatt gacagctttt
    48481 aaactatcaa cggctttttc aattagtgag atatggcgag catttgatag ataagtagcg
    48541 tcatgttcta ccataccagc gttgtcaaag aaaatatcgt taatacgttc ttcgatttta
    48601 tcgatgtttt cattttctaa aaccgaaatt ggaataacat ttttaggtaa ttcgttaact
    48661 tctatttttt gaggaagatc cgttttattg agtaatacga tacgattact ttctttactt
    48721 agttctagaa gggagcggtc ttgtaatgtt agtggttcgg aactattgag aaccaacaac
    48781 acaaggtcgg cttcttctaa agccttcttt gaacgttcaa caccaatttt ttcaacgata
    48841 tcatctgtat cacggatacc agcagtgtca actagtttta aaggaactcc tttaatattg
    48901 acgtattctt caataacatc acgagttgtt ccttcaatat ctgtcacaat cgccttttct
    48961 tcacgtaaga ggttatttag taagctggat ttaccgacat ttggacgacc aatgattgct
    49021 gttgaaagtc cttctcgaag aatttttcca cgtctggcag ttcgaagtaa attttccata
    49081 agtgcttgaa actcttgtgt tttttcacgc atcagtgttg tagtcatctc ttcaacgtca
    49141 tcatattcag ggtaatcgat attaacttca acttgggcta gagtatttaa aatttcttgt
    49201 cgagtgttat tgattagtgt ctttaaagaa ccatctaatt gcttaacggc aatatccatt
    49261 gctttatctg ttttagctct aataagatcc ataacagctt cagcctgagt caagtcaact
    49321 cgaccattta aaaaagcacg tttagtaaat tctcctggtt cagccattcg tgctccatgt
    49381 cgtaaaatta actgaagaat ttcgttagta actgcaattc ctccatgcgt attaatttca
    49441 ataacatctt ctcttgtgaa tgtttttgga gcaagcataa cgcctaacat aacttcatcc
    49501 aagatttcat ttttatctgg gtccacaata tgaccatagt ttaaggtatg ggattggatc
    49561 gcggatagat cttttccacg ataaattttt gatgctatct ttaaggcatc tgtaccagat
    49621 atacggacaa taccaatagc tccctcaccg agaggagttg aaatagctgc gatggtatca
    49681 aattcttttg taatagacat aaagtctctc tctttcttgg aaattactaa cattcattgt
    49741 aacctaaagc cctaaaaggt gcaatcattt gattaaaaga gaagatttcc ataatggttt
    49801 tatttgaagg tatgctataa taaaactatg attatactac aaggaaacaa aattgagcgc
    49861 tcattttcag gtgatgtgct ttttgataat attaatattc aagttgatca acgtgatcgt
    49921 attgctcttg ttggacgtaa tggtgctggc aaatcaacgc ttcttaaaat tttagttggt
    49981 gaagaggctc cgacaaaagg agaaataaat aaaaagcgtg atttgagttt gtcctacttg
    50041 gcacaagata gtcgttttca atctgaaaat acaattttcc aagaaatgtt acaagttttt
    50101 gactcattaa gagaagtaga gaagcgctta cgtgaattgg aattacaaat ggggcaagtt
    50161 tcaggaagtg acctggaaca attgatgaaa acatacgata ttttgtcaga agagtttcgt
    50221 gaaaaaggtg gctttaccta tgaatcagat ataaaggcta ttttaaatgg ttttaagttt
    50281 aacagtgata tgtgggaaat gcctattagt gaattgtctg gcgggcaaaa tacacgatta
    50341 gcgcttgcaa aaatgctact tgaaaagcca gaattacttg ttttagatga accaacgaac
    50401 catttagaca ttgatacgat tgcttggtta gaaaattatc ttgtaaatta tcaaggagcc
    50461 cttattattg tcagccatga ccgctatttt ttagacaagg tagctacagt aacttatgat
    50521 ttaacgacac attctctaga ccgctatgta ggcaattatt cgaaatttat ggatcttaag
    50581 gcagagaaaa tagcgactga agaaaagaat tttgaaaaac aacaaaaaga aattgctaag
    50641 ttagaagatt ttgtacaacg taacattgtt agggcttcaa ccactaaacg agcacaagct
    50701 cgtcgtaaac aattagaaaa gatggaacga ctcgacaaac cgaatgtaga gcaaaaatca
    50761 gctaatatga cttttcatgc tgggaaagtc tcagggaatg ttgttttaac acttgagaat
    50821 gcagctattg gttatgaagg tgtttcttta tcggagccaa tcgatttgga cgtgaagaaa
    50881 tttgatgcta ttgcaattgt aggtccaaat ggtatcggga aatcaacttt aatcaaatcg
    50941 cttgttgggc aaattccttt tatcaaagga gaagcaaaat taggagcaaa tgtcgaaact
    51001 gggtattacg atcaaagtca aagtaattta acaaaaacga atactgtttt agatgagtta
    51061 tgggatgctt tttcaacaac gccagaagtt gaaattagaa accgtttagg agcttttctt
    51121 ttttcaggtg atgatgttaa aaaatcagtt tcaatgctat caggtggtga acgagcgcgt
    51181 ttgctattgg caaaattatc tatggaaaat aataattttc tcatattaga tgagccaaca
    51241 aaccacttgg atattgatag taaagaagtc ttagaaaatg cactcatcga atttgatggc
    51301 acattacttt ttgttagtca tgatcgctat tttattaatc gtgtggcaac taaggttctc
    51361 gaaatttctg ataagggaag tactctctat ctaggagatt atgattatta cctcactaag
    51421 aaagccgaac tagaggaatt agcacgttta aatgaagaag aagtgagtgc ttcaaaaaca
    51481 gaaatcgacg ttacatcaga ttatgagaca caaaaagcaa atcaaaagga atttaggaaa
    51541 attacccgtc gcgttgtaga aattgaggcg cgtttggaag ttcttgaaaa tgatgaaaac
    51601 aatatcaatg gtttaatgtt agaaaccaat gacatcggaa aactctctga tttgcaaaaa
    51661 gagctggaat caatccaaga agaacaattg ctattaatgg aagagtggga aaatctaaat
    51721 atgagactag atttgtgaga aaatttcaaa aacaatagtt tgtgaaaaaa agtacatttt
    51781 aaattgagat gccttttacg aaaacaaaga aagcggttca aattcttgca tcttgtgaaa
    51841 aaaacttata caataattaa gtaataaaga tttttttgga ggttagaatg gtaactctat
    51901 ctaaagaaca acacttggat atgtttctaa aaatgcaacg tattcgtgat gttgatatga
    51961 aatttaacaa attggttcgt cgtggtttcg tccaagggat gactcacttc tctgttggtg
    52021 aagaagctgc ttcagtaggt gctatccaag atttaacaga ttcagatatc attttctcaa
    52081 accaccgtgg acacggacaa actattgcaa aaggtattga tattggtgga atgtttgctg
    52141 aacttgctgg taaagcaaca ggaacatcaa aaggtcgtgg aggctcaatg cacttagcaa
    52201 accttgaaaa aggtaactat ggaacaaacg gtatcgtcgg tggaggatat gcgcttgcag
    52261 taggtgctgc ccttacacag caatatgaag gaactgataa cattgttatc gccttttcgg
    52321 gtgactcagc gactaacgag ggctcattcc atgagtcagt taacttagct gctgtttgga
    52381 acttaccagt tatcttcttt atcatcaaca accgttatgg tatctcaaca gacatcacat
    52441 actcaactaa aattcctcac ctttacatgc gtgctgatgc gtacggtatt ccagggcatt
    52501 atgttgaaga tggtaatgac cttatggctg tttacgagaa aatgcatgaa gttatcaatt
    52561 acgtgcgttc aggaaatgga ccagctattg tagaggttga atcataccgt tggtttggtc
    52621 attcaactgc tgatgctggg gtataccgta ctaaagaaga agttgattca tggaaagcta
    52681 aagatccagt aaaacgctac cgtgcatact taattgaaaa tgaaatcgct actgaagaag
    52741 agcttgcagc tattgaagca caagtgatta aagaagttga agaaggggtt aaattcgctg
    52801 aagaaagtcc attccctgat atgtctgttg catttgaaga cgtttttgtg gactaaaata
    52861 acctaaagta gatgagattt atctcgttct attattcaat tgattaaaaa acggatattc
    52921 ggaggaaata atgagcgaaa ctaaagttat ggctttgcgc gaagcgatta atgtcgctat
    52981 gagcgaagaa atgcgcaaag atgaaaaagt atttttaatg ggtgaagatg taggtgtata
    53041 tggaggagac ttcggtacat ctgttggtat gttagaagag tttggtgcta agcgcgttcg
    53101 cgacacacca atctcagagg ctgcgattgc aggttcagct atcggtgctg cacaaacagg
    53161 tttgcgtcct atcgttgatt tgacattcat ggattttgta acaatcgcaa tggatgctat
    53221 cgttaaccaa ggtgctaaaa cgaattatat gtttggtgga ggtctatcaa ctccagttac
    53281 tttccgtgtg gcatcagggt caggtattgg ttcggctgca caacactcac aatcattaga
    53341 agcttggtta acacacattc caggtctaaa agttgttgca cctggtactg ttaatgagtc
    53401 aaaagcactt ttgaaatctt ctattcttga caataaccca gttatcttct tagagccaaa
    53461 agctctctat ggtaagaaag aagaagttaa tatggaccct gatttctata ttccacttgg
    53521 taaaggggat attaagcgtg aggggactga cttaacaatt gtttcttatg gccgtatgct
    53581 tgagcgtgtt atgcaagctg ctgaagaagt tgctgaggaa ggtattaacg ttgaggttgt
    53641 tgatccacgt acacttattc cgcttgataa agaattaatt atcgattctg ttaagaaaac
    53701 tggtaaattg atcttggtta acgatgctta taaaactggt ggtttcactg gtgaaattgc
    53761 aactatggtt gctgaaagtg aagcatttga ttaccttgat catcctatcg tgcgtcttgc
    53821 aagtgaagat gttccagtac cgtattcacg tgtacttgaa caaggaatct tacctgatgt
    53881 agcaaaaatt aaagatgcta tctataaagt agttaacaaa ggtaaataat tcataaaaca
    53941 aagagggact gaggaatgag tgaaaactta gcctctttcc ttgtttatag gttttaattt
    54001 atatcgttag atgaaacata tatttgacga tatagtttag tgtgaaagga aaactatggc
    54061 tgtcgaaatt attatgccta aactcggggt tgatatgcaa gaaggcgaaa tccttgagtg
    54121 gaaaaaacag gtaggtgatg ttgttaatga aggtgacgtc ctacttgaaa ttatgtcaga
    54181 taaaacaaat atggagattg aagcagaaga ctcaggtgtt ttactaaaaa tcacacatgg
    54241 gaatggagat gttgttcctg taactgagac aattggttat attggtgctg aaggtgaaga
    54301 agtaacagaa gcatcttctt ctgaaaatac atcagttgaa gaaaacgcga cacaagtaac
    54361 ttcagaacca gaaaaagttg aagaaacatc agaaccaagt gtgccagcag ctacaagtgg
    54421 tgaaaaagta cgtgccactc ctgcagctcg taaattagca agagagatgt ctattgacct
    54481 tgcactagta tctggaacag gggcaaatgg tcgcgttcac agagaagatg ttgaaaactt
    54541 caagggtgct caaccacgta ttacaccatt ggcacgtcga attgctgaag atcaaggagt
    54601 tgatatagca gaaatcactg gcagtggtat tcgtggtaaa attgttaaaa atgatgtttt
    54661 ggctgctatg tcaccgcaag cagcagaggc tcctgtagaa actaaagcta caccaacaac
    54721 agaagaaaaa caattaccag aaggtgttga agtcattaag atgtctgcaa tgcgtaaggc
    54781 tatttctaaa ggtatgacta actcttacct cactgctcca agctttacac ttaactatga
    54841 cattgatatg actgaaatga tggctcttcg caagaaatta attgatccaa tcatggctaa
    54901 aacaggcttg aaagtcagct ttactgattt gattggaatg gccgttgtta agacattgat
    54961 gaaaccagag catcgatacc taaatgcttc attaattaac gatgcacaag aaattgagct
    55021 tcataatttt gttaatattg gaatcgcagt tggtcttgat gatggtttga ttgttcctgt
    55081 tgttcacaat gctgatcaaa tgtcactttc tgactttgtt atagcatcta aagatgttat
    55141 taagaaaact caagaaggta aattaaaatc agctgaaatg tcaggttcaa cattctcaat
    55201 tactaactta ggtatgtttg gtactaagac gtttaaccca attatcaatc aaccgaactc
    55261 agcaatcctt ggagtaggag ctactattcc gactccaaca gtggttgatg gtgaaattgt
    55321 agctcgacca attatggcaa tgtgtttaac aattgaccac cgtattgttg atggaatgaa
    55381 tggtgctaag tttatggttg atcttaagaa cttaatggaa aacccatttg gtttacttat
    55441 ttgattccgt aggtcttaat ttgatataat aagttattcg aaaaacgaaa gaggaaataa
    55501 aaaatggctt ttgatgtaat tatgccaaaa cttggggttg acatgcaaga aggcgaaatc
    55561 cttgagtgga aaaaaaatga aggtgacacc gttaatgaag gtgatgtcct tcttgaaatc
    55621 atgtctgata aaaccaatat ggaaatcgaa gcagaagaca ctggtgtact tttaaaaatt
    55681 gtacatcagg caggagatgt tgttcccgtc actgaagtga ttgcctatat cggagaagaa
    55741 ggtgaagaag taggtacgtc atcaccttca gctgatgcaa ctatcacagc tgaagatggt
    55801 caatcagttt ctggacctgc tgctccttca caagagacag ttgcagcagc aactcctaaa
    55861 gaagaacttg cagctgatga gtacgatatc gtagtcgtag gtggtgggcc tgcaggttat
    55921 tacgcagcaa tccgcggtgc tcaacttgga ggaaaaattg ccattgttga gaaaactgaa
    55981 tttggcggta cgtgtttgaa tgttggttgt atccctacaa aaacatatct taaaaatgct
    56041 gaaatcctag atggtttgaa agtagcagct ggacgtggta tcaaccttgc ttcaactaac
    56101 tatgcaattg atatggataa gactgttgcc tttaaaaatt ctgtagttaa gacactgact
    56161 ggtggtgtac gtggtctctt gaaagcgaac aaagttgaaa ttttcaatgg acttggacaa
    56221 gtaaaccctg ataagtctgt tgttattggc gataaagtta tcaaaggtcg taacgtcgtc
    56281 cttgcgactg gttctaaggt atctcgtatt aatattccag gtattgaatc accattagta
    56341 ttaacatcag atgatattct tgatttacgt gaaattccta agtcacttgc tgttatgggt
    56401 ggtggtgtcg taggtattga gcttggtctt gtatgggctt catatggtgt tgacgtaact
    56461 gttattgaaa tggctgatcg catcattcca gcaatggata aagaagtatc gctagaactt
    56521 caaaaaatcc ttgctaagaa gggaatgaag attaaaacat cagttggtgt ttctgaaatt
    56581 gttgaagcca ataatcaatt aacgcttaaa ttaaacaacg gtgaagaagt tgttgctgat
    56641 aaagctcttc tttcaattgg acgtgttcca caaatgaatg gtcttgaaaa tcttgagcct
    56701 gaacttgaga tggaacgtgg acgtattaaa gttaatgctt atcaagaaac ttcaattcca
    56761 ggtatctatg caccaggtga tgttaatgga actcgtatgt tagctcatgc tgcataccgt
    56821 atgggtgaag tagctgctga aaacgcactc ggcggtaata agcgtaaagc acatctagat
    56881 ttcactccag cagctgttta tactcatcct gaagtagcaa tggtggggat gacagaagag
    56941 caagcacgtg aacaatatgg tgacatatta gttggtaaaa atagctttac aggcaatggt
    57001 cgtgctatcg cttcaaacga agcacatggt tttgtaaaag taattgctga acctaagtat
    57061 aaagagattc ttggtgttca cattattggt ccagctgcag cggaattgat taatgaagct
    57121 tctacgatta tggaaaatga gttgactgtc tacgatgtag cacaatctat ccatggtcac
    57181 ccaacattct ctgaagttat gtacgaagct ttcttagatg ttctcggtga agctattcac
    57241 aaccctccaa aacgcaaata attttgttta atatatttga gattgagctt ttatagcttc
    57301 aatctctttt cacgtatata ttgtctgaat attataaaat attagttagg aatttaaaat
    57361 gaaatatatc gttaacacgt ctaatgatcc ggcttacaat gttgctttag aagcttacgc
    57421 ctttcaaaag ttgacagata tcgatgaaat ctttattctt tggattaatg aaccggctat
    57481 cattattgga cgccatcaaa atacaattca agaaattaac aaagagttca ttgataaaaa
    57541 tggcatccat gttgtccgtc gtttgtcagg aggcggagca gtttatcatg acttgaataa
    57601 tttaaactac actattattt ctaataatac acaagaaggt gcctttgatt tccaaacttt
    57661 ttcaaaacca gttattgata ctcttgctaa attaggtgtt aaagcagaat ttactggtcg
    57721 taatgattta gaaatcaatg gacaaaaatt tgcaggaaat gctcaagctt attacaaagg
    57781 tcgtatgatg catcatggtt gtcttttatt tgatgttgat atgtcagtct tagggcaagc
    57841 actcaaagta tctaaagaca aaattgaatc taaagggata aaatctgtac gcgcacgtgt
    57901 tacaaatatt gttgaccatc tttcagataa aattacagta caagaattta gcgatgccat
    57961 tttagctcaa atgaaggaag aatatccaga gatggatgaa tatgttttga gtgatgccga
    58021 gttatcagaa atccaagcta tgcgcgataa tcaatttgct acatgggatt ggacgtatgg
    58081 taaagcacct gaatacacca ttgaacgtgg tgtccgctat ccagctggaa aaattacgac
    58141 ttatgctaat gttgaaaatt caacgataaa atctgtcaaa atctttggtg acttctttgg
    58201 agtgaaacct gtggatgata ttgaaaaaat gttggagggt gtccgctacg attacaaaga
    58261 tgttttagca gcgcttaaaa cagttgatac aagtcaatac ttctcacgta tgacacccga
    58321 agagattaca aaagctattg tagactaaaa tcaaaaaatc tagtcaaagt cttcgccttt
    58381 ggctaggttt tttgtatggt taagtgacgt tatagtagta aaaaaacaac gttattgata
    58441 acgctggttt ttatattatt caaaatctgc ttttgattta acgtctccgt attcttcagg
    58501 aatctctaaa gataaaattt cttcataact agggaggaca atctctttac cgtatttgtt
    58561 acggagggca gttgccacaa gacggtaagc tagattagca ttacgagaaa ggattggacc
    58621 gtgaaagtat gacccaaaaa cattcttata atgaacccct tcagtaccat cttctttgtt
    58681 attcccattt ccataaatga cggttcctaa aggtttttcg tcttctgata aaaaggtacg
    58741 tccttgatga ttctcaaagc cataatatgt ttcattgaat tcatcgttgt gaatcttgat
    58801 atcaccaata tagcgattat tattttggtt tagcgtatag tgtcccataa cgccaatgcc
    58861 ctctattcgt tctccattag cttgaatata gtattgtcct aaaagttgga aacctccaca
    58921 aatagcaagt actacaccat tattttgaat gaatttgtta atgtcttctt tcttactggg
    58981 aagatcgcgt gcaacaatgg cttgttcgta gtcttgtcca ccaccaaaaa atgccatctg
    59041 ataatagttg ggatcaaatc tatcttctaa tgaaacgata tcaaatgtca tttgacatcc
    59101 tagtttttct cccacatatt tcatcattaa gatatttccg ttatcgccat aagtatttaa
    59161 taggttacca taaagatgag ctacattaag agtgtattta taatctttgg tagtaggaga
    59221 ttttagtgag gtataagtca ttaattcatc tcctttctga tagcgttgtg gttagcaaga
    59281 atttcacgaa actccagcat agctgtatat gttgctaaaa tataagcatg ctcagtctct
    59341 tgctgctcaa tcattgtcat aatatcttgc agtttatcag cttgttttat gcgtttctca
    59401 tcataaccag ttacacgtaa gcgacgagca atttcagagt ggcgaacacc accagcaaat
    59461 atttctggga tattcatcgt gagaatagtt tcaaaattag cgtcccaaat ccaagaagta
    59521 tcaataccgt cagcataatt ggcattcagt agtactgaga gagagaacgg gtatggagca
    59581 agtttaatca tatcaagagc ttgactagct cctaccggat ttttaatcag aactaaagta
    59641 cattttttat ttccaattgt aaaggtttct tgacgaccaa aaacggcacg gcttttatca
    59701 aatccatcct taatttgact tggttctaca ccaaaatatt cagcaacaga tactgccgcc
    59761 aaagcattgt aaatattata aaggccaccc acgttgatat tatattgttg gccatcaata
    59821 acgaaacctg atgaagtatt tgtcagatgt gttagccttg ttaaggcata atcaaggttt
    59881 ggacgttcaa aatcacaatt aggacaagtg tagtcaccaa ggttggcata tgtgttcaat
    59941 cgataagtta aaattgcttg acactttgga cagagaatac cctcagtatt atagtgtgct
    60001 aatcgtggct catgtttgtc ggtattaaat ccatagaatt gcacagggtt agtcacgctt
    60061 ttagaattaa aaagtggact gtcaccgtta gctaagatag tagcttgcgg agcgttagca
    60121 gctccatcta aaatcatttg ataagttgtg tagatttcgc cataacggtc catttgatca
    60181 cggaaaatat tggtaaagac gaacaaacta ggctttatat actgtgtaat acggggtaaa
    60241 ctcgcttcgt ctatttcaag gacagctatt tttttaccag atttactttt cttagctgtt
    60301 aggaaggtcg aaacaatacc tgtaatcata tttgctccac ttgggttggt taccacctga
    60361 ccgaaggctt cctttaaaat accaactgtt aaagctgttg ttaaggtttt accgttagta
    60421 ccagtcacaa ctacaatttc ataatctttg gcaattgtat ctagtatatc tttatcgaat
    60481 ttgagtgcta aactacccgg ataagtagag ccacgtccca ttttacttaa aaggtaatga
    60541 gcagacttac cggcagccac tcctagtgct gtatttattt tcataaagat attttatcat
    60601 aaaaagatgc tttcttgcta aagaaaatat tatgaaaaca tggtataatg tagacgatgg
    60661 aaaataagaa atcaaggagt taagcaagaa actcttcgac aggctaccag tagagaggaa
    60721 gatatggata ttttttcagc tattgattca aagttttggg cgagcattat ggaaaatcca
    60781 tggatgatcc ttattcatct catggatatt atcatagtag ctgttctgat ttataaattt
    60841 atcaaagcgc tagcaggcac aaaaattatg tccttaattc agggcgttat tttgtttatc
    60901 atcattagat ttgtgtcaga atggataggg ctaacaacga ttacattttt gatgaatcag
    60961 attgttactt atggtgtaat tgcaggtgtt gtcatctttg ctccagagat tagaacaggt
    61021 ctcgaaaaat ttgggcgaac accacaacta tttacgcaac gttctcaatt aagcagtgat
    61081 gaaaagctag tagatgccct tgttaaggca gttgcttata tgagtccgcg taaaatagga
    61141 gccttgattt caattgaacg tacccaaact ttgcaagagt atatagctac aggtatccca
    61201 ttagatgcag atatttccag tgaattgtta attaatattt tcattcctaa tacaccactg
    61261 catgatggtg ctgttattgt aaaagataaa aaaattgcta cagcatgctc gtacttacct
    61321 ctatcagaat catcttcgat ttcaaaagaa tttggaacac gccacagagc agcgattggt
    61381 ctatcagaga attcagatgc actaacagtt attgtttctg aagagactgg tggtatttct
    61441 gttgctttga aaggtgaatt tttacatgat ctttctaaag atagctttga agcaattctg
    61501 agaactcagt tgattcaaaa tcaagaggaa aactcaaaat tagcttggta taatcaacta
    61561 ttgaggagga agtagacatg aaaaaatttt ttacaaataa attttggtta ggcgttgtct
    61621 cactctttct ggctatttta ttatttttaa cggcaactgc aacaagtatg aaccatcaag
    61681 acaatagtaa gattgcagga gcgtctgaga catatacaca taccctaaca gatgtgccaa
    61741 tagacatcaa atacgatagt gatgattact ttattagtgg atattcttat ggggcagatg
    61801 tctatatgtc atcggttaat cgtgttaagt tagattctga aataaatgag gatacacgaa
    61861 aatttaaagt agtcgccgat ttgacaaata tgaaaccagg gactcacaaa gtcccactaa
    61921 aggtagtaaa tttgccatct ggtgtaaatg ctacggtttc tccaacaaca attacagtaa
    61981 ctatgggtaa aaagaaaaca aaagagtttc cggtttatgg acatgttaat gataagcaaa
    62041 ttaaagctgg ttatgcagtc gataagatgt cagttgatgt gtcaaaagtt aaagttacta
    62101 gtgatgaatc tattatagat cgtattgacc atgtcgctgc caatatccct gatgataaag
    62161 ttcttgatga tgattttaac aagacagtta ctttacaagc tgtaacagca gatggtacag
    62221 tattagcaag tattattcac ccttctaaag caactttatc tgtcaaagta aaaaaattga
    62281 ccaagacagt accgattaat ttgataccag taggacaatt cagcgatagt atttcaaaaa
    62341 ttaactacaa gttgagtcaa gaaaaagcag taatttcagg aacaaaagag gctttagaag
    62401 ctatttctgt tattaatgct gaggtagata tttcagacgt aactaaaaat acagaaaaga
    62461 aaattaatct ttctgcaaac aatgtttcag ttgatcctgc tcaagtaaca gttcaattga
    62521 caactactaa aaaataatag ataataaaaa cgaaaaaaat ggaggtagcg tttttcgcta
    62581 gaaaaaaatt atgggaaaat attttggtac agatggtgtt cgcggtgaag ctaatgttga
    62641 actgacgcca gaattggctt ttaaattagg acgttttgga ggatatgttc taagtcaaca
    62701 cgaaactgat cgtccgagag tttttgtagc tcgtgatact cgtatttctg gtgaaatgct
    62761 ggaatctgct ttgattgctg gtctactttc agtaggtatt gaggtttata aacttggtgt
    62821 tttagccact cctggagtgt cttaccttgt ccgtacagaa aaagctagtg caggggttat
    62881 gatttcagca agccacaatc cagcacttga taatggtatt aagttctttg gtagtgatgg
    62941 ttttaaactt gatgatgatc gtgaattaga aattgaagct ttacttgatg cgaaggaaga
    63001 cactttacca cgtccatcag cacaaggttt aggtacttta gtagattatc ctgaaggact
    63061 tcggaaatat gagaagttta tggaatcaac tggtattgat ttagaaggta tgaaggttgc
    63121 gttggataca gcaaatggag cagcaacagc ctcagctcgg aatatctttt tagacttgaa
    63181 tgctgatata agtgttattg gtgatcaacc agatggtttg aatatcaatg atggtgttgg
    63241 ttcgacacat cctgagcaat tacaaagttt agttagagaa aatggttccg acatcggtct
    63301 agcttttgat ggtgatagtg atcgtttgat cgctgttgat gaaaatggag agattgtaga
    63361 tggtgataaa attatgttta tcattgggaa atatctctct gataaagggc agcttgctca
    63421 gaatacaatc gtaacaacgg ttatgtcaaa tctcggcttt cataaagcgc tcgaccgtga
    63481 aggaattcat aaagcgatta ccgctgtagg agaccgctac gtggttgaag aaatgcgtaa
    63541 atcaggatat aatcttggcg gagagcaatc aggccacgtt attatcatgg attacaatac
    63601 tacaggggat ggacaattaa cagccatcca attgactaag gtcatgaaag aaactggtaa
    63661 gaaattatca gagttagcta gtgaagtgac gatttatcct caaaaattag taaatattcg
    63721 tgttgaaaat aacatgaaag ataaagcgat ggaagttcct gcaattgcgg aaattattgc
    63781 taaaatggaa gaagagatgg atggcaatgg tcgtattcta gtacgtccta gtggtactga
    63841 gcctcttctt agagttatgg cagaagcgcc aacaaatgaa gcggttgatt attatgttga
    63901 tactatcgca gatgtcgttc gtacagaaat tggtttagat taaaacggcg ttattctgct
    63961 ataaagtcgt caatgttata aaataataaa gagatggaat tggagtggag catcaagcat
    64021 acgcctttct ctctcttttt gatttgagaa gggagtttag agaaaatgac taaagaagca
    64081 gaattattat gggctaaaca caagtattta gtcttaagta aatctcagaa aatttatttg
    64141 gatattagac aaactttgaa atcaccaaat tgtaccgttt tagacgttca gtcactcatt
    64201 gaccaagcag tgcttttaga agaatcacca tcacaagtga ctaatgctta tatgcatata
    64261 tggggctatt ttaaaaataa ggcagagcgt caagaaaaag aagaattttt aaccttgctg
    64321 gaaaaataca ggaaaacagg ttatcaacga agaaaactgt tagcttttct aaaacagcta
    64381 ttagctaaat acccaaattc ttatttacaa aactcttcta ttttcgaaga agagtaaaag
    64441 attgatagtt gaggtatata aatgagatta tggcatcaag atattattga gttattgcct
    64501 aggcagcaac ttttaggaca gcatagagag tgttgtgcat tgagagggaa tgggtggggg
    64561 agaaaacatg agactgttga ttatgttttt cgatattcac cttataggtt atttgcatat
    64621 catcaattag ttatggaaga aatgatggag cgtgggtatc gtgtatctaa agaatggcta
    64681 attgcagaat ataggggaat gaagtgccca agatatgata cacttaatcc tgttgacttg
    64741 gagacaccta tctatcccga gcacaatcaa gactatctac aagaatgtct atggaattta
    64801 aaagcaaaag gaattgacct tcctataaat aagataaaat agagatttat ctctatttta
    64861 ttttggaata aaaagctata ttatcaagtt ttagaggagt gtttttttaa gggcaaagtg
    64921 gtaaaataga aacatgttaa agaaaccaac ctcagcatat gtacacattc cattttgtac
    64981 ccaaatatgt tattattgtg atttttcaaa agtttttatt aaaaatcagc ctgtagacgc
    65041 ttatcttcag gttcttatcc gtgaatttcg gtcttatgat attacagaat tgcgcacctt
    65101 atatattgga ggagggacac ctacatctat aagcgctgtg caactggact atttattgac
    65161 ggagttaagt agagatttaa acttaaatac tttagaggag ttcactatcg aagctaatcc
    65221 aggagattta accgttgata aaatagaggt gcttcaaaag tcagctgtca atagagtttc
    65281 tctaggagtt cagaccttta atgataaaca tttaaagcgt attggacgca gtcacaatga
    65341 ggcacaaatt tattctacaa ttgatgctct taaaacagct ggttttcaaa atatctctat
    65401 tgacctaatt tatgccttac ctggacaaac gatggatgat gttagaagta atgtagccaa
    65461 agctctctct ctaaatattc ctcatttgag tttatatagt ttaattttag aacaccatac
    65521 tgtatttatg aataaaatgc gtcgtgggaa attacatttg ccaacagaag atttagaagc
    65581 cgagatgttt gaatatatca tttctgaaat ggaaagaaat ggttttgagc attatgaaat
    65641 ttctaatttt acaaagccgg gttttgaaag tcgtcataat ttgatgtact gggataatgt
    65701 agaatattat ggtgttggtg caggcgcttc tggttatctt gatggtattc gttatcgtaa
    65761 tagagggcca atacaacatt atataaaggg ggtttcagaa ggtaatgcaa ggttatctga
    65821 agaagtatta tcaaaaaatg aaatgatgga agaggagctt ttcctcggtc ttcgtaaaaa
    65881 agagggtgtt tccatcggaa aatttgaaca aaaatttgga acgtcctttg agaaacgtta
    65941 cggtcagatt gttcaagaac tccaaagtga tgggcttttg aaagaaaata atggttttat
    66001 ccagatgact aaaaaaggtc tcttcttagg agatacggtt gccgagaaat ttattgtgga
    66061 gtaaaaaatg ggtttattat atcgagaaac ttacgaagtg cctttttatg aatcagacac
    66121 taatcattat atgaaattac cgcagttgtt ggctttagcg ttacaaattt cagccaaaca
    66181 atctttaaaa ttaggaatag gcgatgacat cgtttttaaa agatacggat tggtttgggt
    66241 cgttacagat tatattattg atattgagcg tttacccaag catgctgaaa agatagtcat
    66301 tgagactgaa gccaaagctc ataataaatt attgtgttac cgctattttt atatttatgg
    66361 tgaagacggg caaaaaataa taacgatttc atctgctttt gtcttaatgg attttaaaac
    66421 acggaagatt catcctgttc ttgatgatat tacttcaatt taccaatccc aaagaatcaa
    66481 aaaagttatt cgagggccga aatatcatcc cattggagat agtaaagtta aacaatacca
    66541 tgttcgttat tttgatttag atatgaatgg acatgttaat aatagtaagt atctagaatg
    66601 gatgtatgat gtgttggact tagattttct aagtagccac attcctaaaa aaattgattt
    66661 aaaatatatt aaggaaattc aatatggaac ggatattaag tctcattggt atcaagatgg
    66721 tcttgtgaca cgtcatgata ttattggtgg cgatgctatt catgctcaag ctaggattga
    66781 atggcaggaa aaaaaggagg attaaatggc ttataaagga tatcttattg atttagatgg
    66841 tactatttac aaagggaaaa gtcgtatccc agcaggagag cgttttatcg aacgtttgca
    66901 agaaaaaggc attccttata tgttagtaac taataatacg actcgtacac cagaaagcgt
    66961 tcaggaaatg cttcgaggct tcaatgttga aacaccactt gaaacaatct ataccgcaac
    67021 tatggctacg gttgactata tgaatgatat gaaccgtgga aaaacagctt acgttattgg
    67081 tgaagaaggg cttaaaaaag ctattgcaga tgcaggttat gtggaggata caaaaaatcc
    67141 agcctatgtg gttgttggtt tagattggaa tgttacttat gataaattgg caactgctac
    67201 gttagcaatt caaaatggtg ctttatttat tggaaccaat ccagatttga atattcctac
    67261 tgaacgtggt ttattacccg gtgctggctc cttaaatgct ttattagaag ccgcaacgcg
    67321 tataaaacca gtctttattg gaaagccaaa tgccatcatt atgaataagg cacttgaaat
    67381 tttaaatatt cctagaaatc aagcagtcat ggtcggtgat aattatttga cagatatcat
    67441 ggctggtatt aataatgata ttgatacttt gttagtaaca acgggtttta caacagttga
    67501 agaagtacca gatttaccaa ttcaaccaag ttatgtttta gcaagtttag atgaatggac
    67561 atttaatgaa ggataagtta ttagtagttt taacttggat ttggattata tcgttagcaa
    67621 ctctagctac aatttatatt gcttggttga tctatcctat agaaattcag tttttaaaat
    67681 tagaaaaggt tgtgtattta aaggctgaaa caatttatta taattttaat aagttgatga
    67741 tatatctgac tcatcctttt atctctgatt taaatatgcc cagctttcct tcttcagagg
    67801 atggtttaaa gcattttgcg gatgtaaaat atctttttac tctagcgcat gggctttttg
    67861 ttatactaac ttttccagtt atttattttt taagacgtgg atggaagcaa aaaagtatct
    67921 ttctatatga aggctttttc aagatagcta ttatgttgcc tatttttatt gtagtatgtg
    67981 cctttctgtt gggttttgac cagtttttca ctctatttca tgaggtgctt tttccaggag
    68041 attctacttg gcaatttaat cctctaacgg atccagtaat ttggatactt cctgaaactt
    68101 ttttcctcca ttgtttcatc atttttttgt taatctatga aacaataact ataattcttt
    68161 tgataatagg tagaaaacat ttaaaattaa gaaaa
//
LOCUS       DATRNK010000004        65043 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.004, whole genome shotgun sequence.
ACCESSION   DATRNK010000004 DATRNK010000000
VERSION     DATRNK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 65043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65043
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.004"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(157..480)
                     /locus_tag="VBC52_000242"
     CDS             complement(157..480)
                     /locus_tag="VBC52_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEO6881850.1"
                     /translation="MINSTVFLAILLGFVVSWVPRVAPFIFVKYKGLPNSLVKFLKFL
                     PLSILFALTFSSFFSVKLGQLPTLKCLDLLAGIPTLVVTIRYRNLFYTVLTGIVTMAL
                     LRLLF"
     gene            complement(470..1162)
                     /locus_tag="VBC52_000243"
     CDS             complement(470..1162)
                     /locus_tag="VBC52_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEO6881851.1"
                     /translation="MEKGFKDGVKDALPTALGYISIGLAFGIVASASDLSAIEVGLMS
                     ALVYGGSAQFAMCALLLAKADLMTITITVFLVNLRNMLMSLHATTIFKSAHLMNQLAI
                     GTLVTDESYGVLLGEALHHKVVSPSWMHGNNVMSYLTWVISTIIGTLLGSTIPNPEMF
                     GLDFALVAMFIGLFVFQLFGMLSDGKRLVVYVLASVGLSYFLLATFLSGALSVLLATV
                     VGCSVGVVLDDK"
     gene            1485..6200
                     /locus_tag="VBC52_000244"
     CDS             1485..6200
                     /locus_tag="VBC52_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO6881852.1"
                     /translation="MNTKQRFSIRKYKLGAVSVLLGTLFFLGGITNVAADSVINKPSD
                     IAVEQQVKDSPTSIANETPTNNTSSALASTAQDNLVTKANNNSPTETQPVAESHSQAT
                     ETFSPAANQPVESTQEVSKTPLTKQNLAVKPTPAISKETPQNIDSNKIITVPKVWNTG
                     YKGEGTVVAIIDSGLDTNHDALQLNDSTKAKYQNEQQMNAAKAKAGINYGKWYNNKVI
                     FGHNYVDVNTELKEVKSTSHGMHVTSIATANPSKKDTNELIYGVAPEAQVMFMRVFSD
                     EKRGTGPALYVKAIEDAVKLGADSINLSLGGANGSLVNADDRLIKALEMARLAGVSVV
                     IAAGNDGTFGSGASKPSALYPDYGLIGSPSTAREAISVASYNNTTLVNKVFNIIGLEN
                     NKNLNNGLAAYADPKVSDKTFEVGKQYDYVFVGKGNDNDYKDKTLNGKIALIERGDIT
                     FTKKVVNAINHGAVGAIIFNNKAGEANLTMSLDPEASTIPAIFTQKEFGDVLAQKSYK
                     IVFNNIKNKQANPNAGVLSDFSSWGLTADGQLKPDLSAPGGSIYAAINDNEYDMMSGT
                     SMASPHVAGATALVKQYLLKEHPELKKGDIERTVKYLLMSTAKAHLNKDTGAYTSPRQ
                     QGAGIIDVAAAVQTGLYLTGGENNYGSVTLGNIKDKISFDVTVHNINKVAKDLHYTTY
                     LNTDQVKDGFVTLAPQQLGTFTGKTIRIEPGQTKTITIDIDVSKYHDMLKKVMPNGYF
                     LEGYVRFTDPVDGGEVLSIPYVGFKGEFQNLEVLEKSIYKLVANKEKGFYFQPKQTNE
                     VPGSEDYTALMTTSSEPIYSTDGTSPIQLKALGSYKSIDGKWILQLDQKGQPHLAISP
                     NDDQNQDAVAVKGVFLRNFNNLRAKVYRADDVNLQKPLWVSAPQAGDKNYYSGNTENP
                     KSTFLYDTEWKGTTTDGIPLEDGKYKYVLTYYSDVPGSKPQQMVFDITLDRQAPTLTT
                     ATYDKDRRIFKARPAVEHGESDIFREQVFYLKKDKDGHYNSVLRQKGEDGILVEDNKV
                     FIKQEKDGSFILPKEVNDFSHVYYTVEDYAGNLVSAKLEDLINIGNKNGLVNVKVFSP
                     ELNSNVDIDFSYSVKDDKGNVIKKQHHGKDLNLLKLPFGTYTFDLFLYDEERANLISP
                     KSVTVTISEKDSLKDVLFKVNLLKKAALLVEFDKLLPKGATVQLVTKTNTVVDLPKAT
                     YSPTDYGKNIPVGDYRLNVTLPSGYSTLENLDDLLVSVKEGQVNLTKLTLINKAPLIN
                     ALAEQTDIITQPVFYNAGTHLKNNYLANLEKAQTLIKNRVEQTSIDNAIAALRESRQA
                     LNGKETDTSLLAKAILAETEIKGNYQFVNASPLSQSTYINQVQLAKNLLQKPNVTQSE
                     VDKALENLDIAKNQLNGHETDYSGLHHMIIKANVLKQTSSKYQNASQFAKENYNNLIK
                     KAELLLSNRQATQAQVEELLNQIKATEQELDGRDRVSSAENYSQSLNDNDSLNTTPIN
                     PPNQPQALIFKKGMTKESEVAQKRVLGVTSQTDNQKVKTNKLPKTGESTPKITYTILL
                     FSLSMLGLATIKLKSIKRE"
     gene            6433..7119
                     /locus_tag="VBC52_000245"
     CDS             6433..7119
                     /locus_tag="VBC52_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6881853.1"
                     /translation="MTKIIYFADDEKNIRDLVVPFLEHDGFTVRAFETGDLLLEAYKN
                     QKPDLVILDIMMPGTNGLDVMKSIRQYDNVPIIMLTARDSDVDFITAFNLGTDDYFTK
                     PFSPIKLSLHVKALFKRLDEKAIKNDTQYQFLDLTLDTEKRIALLSNEEMPLTKTEFD
                     FLLVLIEKPETAFSRETLLNRIWGFDDIESRAVDDTIKRLRKKFKQYHSQVSIKTVWG
                     YGFKLIKEDK"
     gene            7116..8504
                     /locus_tag="VBC52_000246"
     CDS             7116..8504
                     /locus_tag="VBC52_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6881854.1"
                     /translation="MKKKSLISNLILINSITLFLGFYLIYFSVNYVIKDYVHQMTRNS
                     MQANFTILDSIYNNKKIPDENMVNQDSIFVWSYYAIFDQHYRMKYGTDNHKKESHDII
                     RYLTQKRLWQISKEKDGMFVTIKKKTYYVMTKDYSGILVDGSIKKVPKAQSQLFHVIN
                     FSDITYTQHLITKINHFLIVILVLTYIPMLFIMRKTFTGIRESIQSVQTYISSLWKNQ
                     GNHQSSQKEIVFSDFDPLLLESQEMANRIYQAEESQRNFFQNASHELRTPLMSIQGYT
                     EGVQEGIIDAELAHSVILQESKKMKQLVDDIILLSKLDSNLSDQKDEFSLNELLNSII
                     AYFKPLANKQKISITYRPDEHEKLLKGNEELIQRAINNILSNALRYAVSHIEISYTNQ
                     KLTISNDGPAISKEDLPYIFDRFYKGHGGQTGIGLAMTKEIIKQHHGNIIAESDSTST
                     TFTIYFNCHKIA"
     gene            8597..9205
                     /locus_tag="VBC52_000247"
     CDS             8597..9205
                     /locus_tag="VBC52_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome assembly protein"
                     /protein_id="HEO6881855.1"
                     /translation="MLHSKKIHSLSLIAVLSLATYTSLQPNHVTAEQSQKTSTVHMSQ
                     KTIEHKLKVADKEAAPLYAKIDHIQRHIEVKKAKDLKVIELYINKDINQLEKQNKRLL
                     TKFYTSIDNQTWDSTSEVKKLIDKTNLSTNEKNRLKLYFEQRAYLETRLNDRYQKFDN
                     SIENQNKELKILTSKIEKIYQKHGITKEVLKTYYAKKTVRAD"
     gene            complement(9295..11796)
                     /locus_tag="VBC52_000248"
     CDS             complement(9295..11796)
                     /locus_tag="VBC52_000248"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEO6881856.1"
                     /translation="MTFYNHKEIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVAEYKRQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFKLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITKENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQVLTASGESISYVPWPSYDESKLVENEIEIVVQIKGKVKAKLVVAKDLSREEL
                     QDLALANEKVQSEIAGKDIIKVIAVPNKLVNIVVK"
     gene            12173..13420
                     /locus_tag="VBC52_000249"
     CDS             12173..13420
                     /locus_tag="VBC52_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6881857.1"
                     /translation="MSPLNINKNNWRALIAAIVASGTDDLNIMFLAFSMSTIITDLHL
                     SAAQAGWIGTITNLGMLVGGLIFGLLADRYNKFKVFKWTILIFSIATGLVFFTTNLSY
                     LYIMRFIAGIGVGGEYGIAIAIMAGIVPANKMGRISSLNGIAGQVGSISSALLAGWLA
                     PALGWRGLFLFGLLPIVLVLWMQFAIDDKDILDQYNTDADDEPLDISIKALFDTPVLA
                     TQSLALMVMTTVQIAGFFGMMNWLPTIIQTNLNVSVKNSSLWMIATILGMCLGMLVFG
                     QLLDKFGPRLVYGCFLLSSAICVYLFQFATTMPSMIIGGAVVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNLILNVGRAIGGFSSVIIGMILDVSNVSMVMLFLASLYIVSFLSML
                     SIKQLKHQKYHTNLTQLDVKPTD"
     gene            complement(13463..14308)
                     /locus_tag="VBC52_000250"
     CDS             complement(13463..14308)
                     /locus_tag="VBC52_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HEO6881858.1"
                     /translation="MLSVGLVLEGGGMRGLYTAGVLDAFLDAGIKVDGIISVSAGALF
                     GVNFVSRQRERALRYNKKYLSHPKYMSLRSWLRTGNFVNKDFTYYEVPMKLDVFDDEA
                     FKKSSIDFYAVATEMTSGKPEYFKIDSVFEQMEILRASSALPVVSKMVVWQGKKYLDG
                     GLSDSIPVDFARGLGFDKLIVVMTRPLNYQKKPSSGRLYKTLYRKYPNFVKTASNRYQ
                     QYNNSLEKVMSLEKTGDLFAIRPSKSLVIGRLEKNPDKLDSIYQLGMKDAKSGMPELN
                     SYLMK"
     gene            complement(14446..14985)
                     /gene="nusG"
                     /locus_tag="VBC52_000251"
     CDS             complement(14446..14985)
                     /gene="nusG"
                     /locus_tag="VBC52_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEO6881859.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGKTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRSILISMGQTVDVFDTNIKEGDVVQIIDGAFIGQEGRVVEIENNKVKLMI
                     NMFGSETQAELELYQVAEL"
     gene            complement(15191..17641)
                     /locus_tag="VBC52_000252"
     CDS             complement(15191..17641)
                     /locus_tag="VBC52_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEO6881860.1"
                     /translation="MNNNEKKVKYFLRKTAYGLASMSAAFAVCSGIVHADTSSGISDS
                     IPHKKQVNLGAVTLKNLISKYRGNDKAIAILLSRVDDFNRASQDTLPQLINSTEAEIN
                     NTLPQGRIIKQSIPVVRLKVERLGSGAIKAESINNIKAESINKIQGKSTNTIKAESIN
                     KIKVESINTIKAESINKIQAKPINTIKAESINTIKAESIHKIKPQSIKSTSATHVKVS
                     DQELAKQSRRSQDIIKSLGFLSSDQKDILVKSISSSKDSQLILKFVTQATQLNNAEST
                     KAKHMAQNDVASIKNISLEVLEEYKEKIQRASTKSQVDELVAEAKKVVNSNKETLVNQ
                     ANGKKQEIAKLENLSNDEMLRYNTAIDNVVKQYNEGKLNITDAMNALNSIKQAAQEVA
                     QKNLQKQYAKKIERISLKGLALSKKAKEIYEKHKSILPTPGYYADSVGTYLNRFRDKR
                     TFGNRSVWTGQSGLDEAKKMLDEVKKLLKELQDLTRGTKEDKKPDVKPEAKPEAKPNI
                     QVPKQAPTEAAKPALSPEALTRLTTWYNQAKDLLKDDQVKDKYVDILAVQKAVDQAYD
                     HVEEGKFITTDQANQLANKLRDALQSLELKDKKVAKPEAKPEVKPEAKPDVKPDVKPE
                     AKPEAKPEAKPEAKPEAKPEAKPEAKPEVKPDVKPEAKPDVKPEAKPDVKPEVKPDVK
                     PEAKPDVKPEAKPDVKPEVKPEAKPEVKPDVKPEARPEAKPEVKPDVKPEAKPEAKPE
                     VKPDVKPEAKPEAKPATKKSVNTSGNLAVKKAIENKKYSKKLPSTGEAASPLLAIVSL
                     IVMLSAGLITIVLKHKKN"
     gene            complement(17952..18125)
                     /gene="secE"
                     /locus_tag="VBC52_000253"
     CDS             complement(17952..18125)
                     /gene="secE"
                     /locus_tag="VBC52_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEO6881861.1"
                     /translation="MFVKGIFQVLRDTTWPNRKQRWKDFISILEYTVFFTIVIYIFDK
                     LLAAGVMDLINRF"
     gene            complement(18161..18313)
                     /gene="rpmG"
                     /locus_tag="VBC52_000254"
     CDS             complement(18161..18313)
                     /gene="rpmG"
                     /locus_tag="VBC52_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6881862.1"
                     /translation="MAQKKASLACTECGSRNYSIGVSSTPKPTRLEVNKFCKYCKKYT
                     IHKETR"
     gene            complement(18362..20683)
                     /locus_tag="VBC52_000255"
     CDS             complement(18362..20683)
                     /locus_tag="VBC52_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6881863.1"
                     /translation="MKLFDKFIDLFRVDEDNDEMTRKNEQETREETSNLDGEEVYDID
                     DITRPSKSQYQRGIRHQKKNAKSRPEWLQKVDRYLPSPKNPIRRFWRRYRIGKLLFIA
                     LMAFILIFGSYLFYLSKTATVSDLQSALKTTTTIYDKNKEYAGKLSGQKGTYVELNAI
                     SDHLKNAVIATEDRTFYENNGVNFKRFFLAVATLGKFGGGSTITQQLAKNAYLSQDQT
                     IKRKAREFFLALELTKKYSKAEILTMYLNNSYFGNGVWGVEDASRKYFGTSAANLTVD
                     EAATLAGMLKGPEVYNPYYSVENATNRRDTVLAAMVDAGKLTKSQAKEAASIGMKNRL
                     ADTYAGKINDYRYPSYFDAVVNEAIDTYGISEKDIVNNGYKIYTALDQNYQSGMQKTF
                     DDTSLFPVSDYDGQSAQGASVALDPKTGGVRGLVGRVQSTKDAQFRSFNYATQSKRSP
                     ASTIKPLVVYSPAIASGWSIDKELPNKVQDFHGYKPSNYGGIETESIPMYQALANSYN
                     IPAVYTLDKLGINKAFTYGRKFGLNMSSANKELGVALGGSVTTNPLEMAQAYSTFAND
                     GIMHRAHLITRIETANGKLVKQFTDKPKRVISRSVASKMTSMMLGTFSNGTAINANVY
                     GYTMAGKTGTTETDFNPNLSGDQWVVGYTPDVVISQWVGFKKTDKHHYLTDSSAGTAS
                     NIFSTQASYILPYTKGSSFTHIENAYFQNGIGSVYNAQDASNTTNQESRSIINDLKDS
                     ASKAAQDISRAVEDSNFQEKVKDAWNSLKDYFR"
     gene            20727..21611
                     /locus_tag="VBC52_000256"
     CDS             20727..21611
                     /locus_tag="VBC52_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6881864.1"
                     /translation="MSFSLKIRNPYGEHTVKELLEDYFLIPRKIRHFLRVKKHVLINN
                     EFINWQTVVQENDTITLIFDDEDYPTKKIPLGRAELIDCLYEDEHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSAYSGQTCYVVHRLDMETSGAVLFAKNPFILPLINQRLERKEIWR
                     EYWALVEGKFSPKHQVLRDKIGRNRHDRRKRIIDSKNGQHAMTIIDVLKYTQNSSLIK
                     CRLETGRTHQIRIHLSHHGHPLIGDPLYNPSSNNERLMLHAHRLTLSHPLTCETISVE
                     APSSTFEKILNNYKKGVG"
     gene            complement(21636..23276)
                     /locus_tag="VBC52_000257"
     CDS             complement(21636..23276)
                     /locus_tag="VBC52_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881865.1"
                     /translation="MFDFFKKKVVKVCLVIFGIVLVSLLSLGFFYFSKGQVLSRFVAA
                     RSRTSGQAFDNIKEYMVWSDTGESITNDEANYANFEPLSKSEARKLGQEIKEGNKNDS
                     MYLKRVGSRLGIFPDYRIANKPMSLTLKTNVPKLDVLLNQKKVATSNSDHFSVTVERL
                     PRTHYTASLEGTSDGKEIKLKKDYDGKNQTIDLSVAFKSFTVTSNLMDGNLYFGDNRI
                     AKLKDGSYSVENYPVTDGSKAYIKKVFNDGEITSHKQKLISIADNQTIKLDVDGLLNE
                     KEAGQKLITAFNQLILYVSTGQDPQTLGTVFEKGAENDFYKGLKESIKAKFVTDNRKA
                     SHFTIPNIVLNKMTQVGKESYQVNFAADYDFNYDKSTDPDKKTYGHIIQNLTGNFIMK
                     KSGNSYLISNDGKKDITVAKETNKVKADPVSIFPENLVGSWKGEVEDGTVTMTFDKDG
                     KVTQKKVYKDSKSKESNHSAKVTKLEDKGNGLYLYQYESGTDTTTFVTGGIGGLKVKY
                     AYGIKIEGNKIIPVIWQTSSDGEFDYHKPLLSKPLTKQ"
     gene            complement(23446..24657)
                     /locus_tag="VBC52_000258"
     CDS             complement(23446..24657)
                     /locus_tag="VBC52_000258"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO6881866.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFEDTVLNKLDQAGIDTYAVGKINDIFNGSGINHDMGHNKSNSHGIDTL
                     IKTMGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDERLPEIISAMRDKDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFADISATVADNFGVDTA
                     MIGESFLQDLV"
     gene            complement(24724..25395)
                     /gene="deoC"
                     /locus_tag="VBC52_000259"
     CDS             complement(24724..25395)
                     /gene="deoC"
                     /locus_tag="VBC52_000259"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEO6881867.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIKNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAACQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVGEGVKIKAAGGISSLEDAEKFIALGASRLGTSRIIKIVKNQKV
                     EEGTY"
     gene            complement(25425..26627)
                     /locus_tag="VBC52_000260"
     CDS             complement(25425..26627)
                     /locus_tag="VBC52_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEO6881868.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQVVSVVSTGVTKVINCGQAGLNFVFGSLADSGAKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILLPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRGTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            complement(26648..27427)
                     /gene="udp"
                     /locus_tag="VBC52_000261"
     CDS             complement(26648..27427)
                     /gene="udp"
                     /locus_tag="VBC52_000261"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEO6881869.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            27585..28322
                     /locus_tag="VBC52_000262"
     CDS             27585..28322
                     /locus_tag="VBC52_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6881870.1"
                     /translation="MPKNELNNKLNKLKHVQVYNKIFNMIQDGTYSPGMQLPSEPELA
                     GQLNVSRATLRKSLALLQEDHLVKNIRGKGNFIRENSSNLSENGYENRQHPIKTCLTS
                     KITEVELEFRVEVPAEAITASLKQETPVVVIADRWYHTDDGPLAYTLSFIPIELISDA
                     EISLHDTKQLLNFIEEGIYQEGISSHSQSHLGYATSGNFSATKYTLSDHGQFILIQET
                     IFNQEKILMCNKHYVPIEHFELSITSH"
     gene            28335..28697
                     /locus_tag="VBC52_000263"
     CDS             28335..28697
                     /locus_tag="VBC52_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881871.1"
                     /translation="MQNRLLKVAFFITFTELIMGLIVGAYFKLSYFLAPPKEPSMGAG
                     YGMLFAMLFILISYLILLAIIQFLAYQSQSKIIAIIVLLLFFLPLLANIGWPILMIFL
                     PQVVVTIISLLSQHKTPL"
     gene            complement(28732..30354)
                     /gene="groL"
                     /locus_tag="VBC52_000264"
     CDS             complement(28732..30354)
                     /gene="groL"
                     /locus_tag="VBC52_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEO6881872.1"
                     /translation="MAKDIKFSADARSAMVRGVDILADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPIGIRRGIETAVSAAVEELKEIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVSELENPYI
                     LITDKKISNIQEILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVVTEDLGLDLKDATMQVLGQSAKVTVDKDSTVIVEGA
                     GDSSAIANRVAIIKSQMEATTSDFDREKLQERLAKLAGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVSGGGTALVNVIEKVAALKLNGDEETGRNIVLRALEEPVRQ
                     IAYNAGYEGSVIIERLKQSEIGTGFNAANGEWVDMVTTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPEAPTAPAMDPSMMGGF"
     gene            complement(30450..30734)
                     /gene="groES"
                     /locus_tag="VBC52_000265"
     CDS             complement(30450..30734)
                     /gene="groES"
                     /locus_tag="VBC52_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEO6881873.1"
                     /translation="MLKPLGDRVIISFVETEEKSVGGFVLAGASHDATKTAKVLAVGD
                     GIRTLTGELVAPSVAEGDTVLVENGAGLEVKDGNEKVTVVRESDIVAVVK"
     gene            complement(30909..31712)
                     /locus_tag="VBC52_000266"
     CDS             complement(30909..31712)
                     /locus_tag="VBC52_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6881874.1"
                     /translation="MSLLELVNLHKTFEKGTVNENHVLRGLDLTIEDGDFISVIGGNG
                     AGKSTLLNCIAGLISIDQGAITLDNQSITKDSVEKRSKDISRVFQDPRMGTATNLTIE
                     ENMAIAHKRGNKRHIFRQSVTDDDRQLFKKSLSQLGLGLENRMKTDAAFLSGGQRQAL
                     TLAMATLVRPKLLLLDEHTAALDPKTSDMVMELTQKVIEEQRLTALMITHNMEHAIAY
                     GNRLVMLYHGKIVVDVKGEAKRNLTVAELMELFHKNSGQQLIDDALVLG"
     gene            complement(31717..33591)
                     /locus_tag="VBC52_000267"
                     /pseudo
     CDS             complement(31717..33591)
                     /locus_tag="VBC52_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364177.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(34056..34853)
                     /locus_tag="VBC52_000268"
     CDS             complement(34056..34853)
                     /locus_tag="VBC52_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6881875.1"
                     /translation="MVRLIFSDIDGTLINSNFKVTPKTRQGIKQIVAQGATFVPISAR
                     MPEAITPIMEQIGIDSYIISYNGALIQDMQQKTIASHTMDGQVALQVCSYVSKHYSKI
                     AWNVYRYHEWYSCDKENEWVQKEEEIVGLQSKEMSLMELEKQDRIHKLLLMGEPSLMG
                     ELENTLKAQYPHLSIAQSAPYFIEIMAPGIEKGKSAKTLADYLDISLADSIAFGDNYN
                     DLNLLEIVGKGFVMANAPKDLQERIGNVTQDNDNDGIYYALVEYMEN"
     gene            35039..35899
                     /locus_tag="VBC52_000269"
     CDS             35039..35899
                     /locus_tag="VBC52_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6881876.1"
                     /translation="MPYPYKANHSIESITLKVNDLENLVNFYSDIIGLTVIDKSSTRA
                     LLGVNQKIPLIILEKTELEKHSTYGLYHTAILVPDEYHLSLALNHLLSQHIPLEGGAD
                     HGYSNAIYLSDPEGNGIEIYNDKDISMWDIRESGQIIGITERLDIDNLLDSLVNVPNN
                     YKLSEKTRIGHIHLSVKDAKISSKLYQNVFGFDEKFAIPTASWIASGNYHHHLAFNNW
                     AGPNLSKNQEDRPGIFLLTIAYNDDNLFRDSLKKAQLYQLTFLEKQDHYYIIEDFDGI
                     RIKVVLKPEN"
     gene            35942..36673
                     /gene="yaaA"
                     /locus_tag="VBC52_000270"
     CDS             35942..36673
                     /gene="yaaA"
                     /locus_tag="VBC52_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEO6881877.1"
                     /translation="MIKILIPTAKEMKVCQNIARPKLSAQTQIIIDYFSTLTVSDLED
                     IYRINTSAARCEAQRWQDFKCKQLTLNPAIKLFNGLMYRNIKRHNLSISEAQFMENSV
                     FITSALYGIIPAMTLISPHRLDFNTKIKINNNSLKVFWRENYDTFMQSDDIMVSLLSN
                     EFETVFSPKERQKLIHLNFIEDRDGQLKTHSTISKKARGKCLTAMMENNCQTLEHLKQ
                     LRFDGFCYDNELSDSKQLTFVKKQT"
     gene            complement(37030..37647)
                     /gene="nrdG"
                     /locus_tag="VBC52_000271"
     CDS             complement(37030..37647)
                     /gene="nrdG"
                     /locus_tag="VBC52_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEO6881878.1"
                     /translation="MSEEASWNTPKPGEWKSEELSHGHIIDYKAFNFVDGEGVRNSLY
                     VAGCMFHCKGCYNTATWSFRAGIPYTKELEDQIMKDLAQPYVQGLTLLGGEPFLNTGI
                     LLPLLQRIRRELPEKDIWSWTGYTWEEMMLETQDKLEMLSLIDILVDGRFDQSKRNLM
                     LQFRGSSNQRIIDVQKSLKEGEVVIWEGLNDGDNSYEQVKRDDLL"
     gene            complement(37720..38211)
                     /locus_tag="VBC52_000272"
     CDS             complement(37720..38211)
                     /locus_tag="VBC52_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6881879.1"
                     /translation="MKLRRPVLEDKEEILAMYKEFQKESSSMDGGFYEPTMHFEDWLD
                     HNLNMELGIGVPDNFVPYIQFVSFDNDNNAIGFLNLRLRLNDTLLEKGGHIGYSIRPR
                     QRGKGYAKEQLKLGIEQAHLKNINEILVTCHVDNDASKSVILANGGVLEDCRHQTERY
                     WIT"
     gene            complement(38220..39152)
                     /locus_tag="VBC52_000273"
     CDS             complement(38220..39152)
                     /locus_tag="VBC52_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6881880.1"
                     /translation="MLQLGIVGLGGISQKAYLPYMRQVTGVHWHLFTRQKQILEEVNM
                     LFGSSTAYDSLDSLAEHPLDGVFIHVATSAHFDIAKLFLKKGIPVFMDKPLTEDYTST
                     KALYDLAKDHKTFLMAGFNRRFAPRIMEMKKVEDKNHIRTFKNAVNAPADFQYKLFDM
                     FIHPLDTALFLTNNVVKRGYFVTKRDGNKILQVSVTLETDSEIIEASMNLQSGSRREI
                     IEIESPEVTYSLDDLSNLSVIDGFDRRAIGFGSWASTLEKRGFEPMIDAFIQAITTGV
                     NPISPKSSLLSHFICDQINKANAPFGMLNLKIME"
     gene            complement(39165..39308)
                     /locus_tag="VBC52_000274"
     CDS             complement(39165..39308)
                     /locus_tag="VBC52_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881881.1"
                     /translation="MGKYQLDYKGQAQVQKFHEKHSTGENANQKSRLKDLRKQFLEKA
                     KKK"
     gene            complement(39383..41581)
                     /gene="nrdD"
                     /locus_tag="VBC52_000275"
     CDS             complement(39383..41581)
                     /gene="nrdD"
                     /locus_tag="VBC52_000275"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEO6881882.1"
                     /translation="MMVLERERFMTESDIKVIKRDGRLVSFDKYKIYTALLKASNKVI
                     KMSPLVEAKLEMIADHVIAEIYNRFKDNIKIYEIQNIVEHKLLEANEYAIAQEYINYR
                     TQRDFERSQATDINFSIGKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAQLNYQKHLKDAKEWVIEDKQEDYAR
                     AKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTNWFEREIQKAILKIRIQGL
                     GSEHRTAIFPKLIFTLKKGLNLEENSPNYDIKQLALECATKRMYPDVLSYDKIIDLTG
                     SFKAPMGCRSFLQGWRDANGQDVTSGRMNLGVVTVNLPRIAMESNGDMDKFWEIFNER
                     MSIARDALVYRVERVKEAIPANAPILYQYGAFGERLGKYDNVDRLFNHRRATVSLGYI
                     GLYEVASVFYGGDWEDNHQAKAFTVDIVRKMKQLCADWSDEYDYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKNPTPFEKLDFEKIYPETGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYQCQFEGDFTPTDRGFTCPNCG
                     NSDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSSIKNQGN"
     gene            complement(41679..43220)
                     /locus_tag="VBC52_000276"
     CDS             complement(41679..43220)
                     /locus_tag="VBC52_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881883.1"
                     /translation="MVENLEKPIGVSYKNSPSMSKRTAIRMKKSSRFSILLYSVLSTL
                     LAIANPLLTYFANGLQTQNLYTGLMMTKGQIPYSDVFATGGFLYYVTIALSYLLGSSI
                     WLLIVQFIAYYVSGIYFYKLVYYVAQSEIVSIGMTLIFYIMNIVLGFGGMYPIQWALP
                     FMLISLWFLIKFCVDNIVDEAFIFYGILAAFSLFIDPQTLIFWLCSFVLLTATNIKQK
                     QSLRGFYQFLCVVFGMILIAYTVGYFMFNLQIISSYIDKAIFYPFTYFARTNHSFLLS
                     LAIQIVVLLGSGCLFGLWDFIQNRKKASYQIGLNFIACIFIIYAIMAIFSRDFNLYHF
                     LPALPFGLLLTSNKITILYQKVIDRRSHRRQYFSGKSLIVDLFVKKTYYLPLLLVSLS
                     IGLLVYNTYQNVTLSKERRDISHYLTTKIDRDGKIYVWDKVASIYSQTRLKSASQFVL
                     PHINTAQKNNEKILKDELLQHGAKYFILNKNEKLPNELKSDIKKHYQEVPLSNITHFV
                     LYRFK"
     gene            complement(43471..43788)
                     /locus_tag="VBC52_000277"
     CDS             complement(43471..43788)
                     /locus_tag="VBC52_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEO6881884.1"
                     /translation="MAHNHNHDHNHEHQHEVITLVDENGNETLFEILLTIDGREEFGK
                     NYVLLIPAGAEEDEQGEIEIQAYSFTENADGTEGDLQPIPEDSDAEWDMIEEVFNSFL
                     DEE"
     gene            complement(43814..44233)
                     /gene="ruvX"
                     /locus_tag="VBC52_000278"
     CDS             complement(43814..44233)
                     /gene="ruvX"
                     /locus_tag="VBC52_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEO6881885.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEESGNFGFDR
                     LAELVKEYKVDKFVVGLPKNMNNTSGPRVEASQAYGDKITELFNLPVEYQDERLTTVQ
                     AERMLVEQADISRGKRKKVIDKLAAQLILQNYLDRMF"
     gene            complement(44233..44499)
                     /locus_tag="VBC52_000279"
     CDS             complement(44233..44499)
                     /locus_tag="VBC52_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEO6881886.1"
                     /translation="MGFTDETVRFRLDDSNKVEISETLTAVYRSLEEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDL"
     gene            complement(44701..45099)
                     /gene="spx"
                     /locus_tag="VBC52_000280"
     CDS             complement(44701..45099)
                     /gene="spx"
                     /locus_tag="VBC52_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEO6881887.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHQLPYKEQNLGKESLTRDEILE
                     ILTKTESGIESIVSSKNRYAKALNCNIEELSVNEVIDLIQENPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNVENAEARLRAAL"
     gene            complement(45315..46454)
                     /gene="recA"
                     /locus_tag="VBC52_000281"
     CDS             complement(45315..46454)
                     /gene="recA"
                     /locus_tag="VBC52_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEO6881888.1"
                     /translation="MAKKTKKAEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIVEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYSSVRLDVRGNTQIKGTGEHKDHNVGKETKIKVVKNKVAPPFREAF
                     VEIMYGEGISRTGELIKIASDLDIIQKAGAWYSYNGEKIGQGSENAKKYLADNPAIFD
                     EIDHKVRVHFGMTEDDSPVQSELVEEKNEADDLVLDLDNAIEIEE"
     gene            complement(46528..47787)
                     /locus_tag="VBC52_000282"
     CDS             complement(46528..47787)
                     /locus_tag="VBC52_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEO6881889.1"
                     /translation="MRAEIIAVGTEILTGQIVNTNAQFLSEKMAELGIDIYFQTAVGD
                     NEERLLSLLDIASQRSQLVILCGGLGPTEDDLTKQTLAKFLGKSLTVDLLASQKLDRF
                     FASRPQFARTPNNERQAQLVEGSIPLQNLTGLAVGGIVTSKGVQYMVLPGPPSELNPM
                     VMEQVVPILSNNGTKLYSRVLRFFGIGESQLVTILEDIIKNQTDPTIAPYAKVGEVTL
                     RLSTKAENQDEADFKLDSLEKEILALKTLDNRKLKDLLYGYGDNNSMARTVLELLKVQ
                     NKTITAAESLTAGLFQSQLAEFSGASQVFNGGFTTYSMEAKSQLLGIPKKKLQEYGVI
                     SHFTAEAMAQQARQLLKADFGIGLTGVAGPDELEGYPAGTVFIGIATPEGVSSIKVSI
                     GGKSRSDVRHISTLHAFDLVRRALLKI"
     gene            complement(47876..48427)
                     /locus_tag="VBC52_000283"
     CDS             complement(47876..48427)
                     /locus_tag="VBC52_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEO6881890.1"
                     /translation="MKRCSWVNLDNPLYVAYHDKEWGRAVHDDHVLFELLCLETYQSG
                     LSWETVLNKRQEFRQVFHHYNIEKVAAMSDADLEIILQNPRVIRHRLKLFSTRQNARS
                     IILIQKEFGSFDRYIWSFVDNKVQVNSVNNYNDVPASTTLSERLSKDLKKRGFKFVGP
                     TCLYSFIQAAGMVNDHENICDFK"
     gene            complement(48450..49040)
                     /gene="ruvA"
                     /locus_tag="VBC52_000284"
     CDS             complement(48450..49040)
                     /gene="ruvA"
                     /locus_tag="VBC52_000284"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEO6881891.1"
                     /translation="MYDYIKGKLSKITAKFIVVETAGLGYVIYVANPYSFSGYVNQEV
                     TIYLHQVIRDDAHLLFGFHTENEKEIFLNLISVSGIGPTTALAIIAVDDNEGLVSAID
                     NSDIKYLTKFPKIGKKTAQQMILDLSGKFVEASGESATSRKVSSEQNSNLEEAMEALL
                     ALGYKATELKKVKAFFEGTNETVEQYIKSSLKMLMK"
     gene            complement(49042..50274)
                     /locus_tag="VBC52_000285"
     CDS             complement(49042..50274)
                     /locus_tag="VBC52_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6881892.1"
                     /translation="MKEFFALPKQLQLRELLRFISITVGSAIFPFMAMYYVQYFGNLV
                     TGILIIITQLSGFVATLYGGHLSDAIGRKKVVIVGSLLATIGWAITIAANVPNHITPH
                     LTFVGILIIEIAHQFYFPAYEAMTIDLTNEQNRRFVYTIGYWLVNIAVMLGSGIAGIF
                     YDHHFFELLIVLLIISAICCFVVYFKFDETKPQEGTFKHDKGVLGTFKNYSQVLVDKA
                     FVVYTLGAIGSSVVWLQVDNYFSVNLKQNFEVVSILGHTITGAKMLSLAVFTNTLLIV
                     LLMTTINKFIENWPLKRQLILGSLICGFGMLFNISLNTFGAILIAMTFFTFGEMIYVP
                     ASQVLRAEMMVEGKIGSYSGFLAIAQPVASVLAGAMVSLSYFTGKIGVQITLTIFMLA
                     GLVLILYATKMKNIEIGK"
     gene            complement(50306..52279)
                     /gene="mutL"
                     /locus_tag="VBC52_000286"
     CDS             complement(50306..52279)
                     /gene="mutL"
                     /locus_tag="VBC52_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEO6881893.1"
                     /translation="MSKIIELPDILANQIAAGEVVERPSSVVKELVENAIDAGSSQIT
                     IEVEESGLKKIQITDNGEGMTSEDAVLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     IASISLMTIKTATEQGKQGTLLVAKGGNIEKQEVVSSPRGTKILVENLFFNTPARLKY
                     MKSLQSELAHIIDIVNRLSLAHPEVAFTLINDGKEMTKTSGTGDLRQAIAGIYGLNTA
                     KKMIEISNADLDFEISGYVSLPELTRANRNYITLLINGRYIKNFLLNRSILDGYGSKL
                     MVGRFPIAVIDIQIDPYLADVNVHPTKQEVRISKERELMSLISSAISESLKQYDLIPD
                     ALENLAKTSTRSVDKPIQTSFSLKQPGLYYDRTKNDFFIDADTVSEPIANFTNLDKSD
                     GSVDNDVKNSVNQGATQSPNIKYASRDQADSENFIHSQDYLSSKQSLNKLVEKLDSEE
                     SSTFPELEFFGQMHGTYLFAQGNGGLYIIDQHAAQERVKYEYYREKIGEVDNSLQQLL
                     VPFLFEFSSSDFLQLQEKMSLLQDVGIFLEPYGNNTFILREHPIWMKEEEVESGVYEM
                     CDMLLLTNEVSVKKYRAELAIMMSCKRSIKANHTLDDYSARHLLDQLAQCKNPYNCPH
                     GRPVLVNFTKADMEKMFKRIQENHTSLRDLGKY"
     gene            52505..52708
                     /locus_tag="VBC52_000287"
     CDS             52505..52708
                     /locus_tag="VBC52_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO6881894.1"
                     /translation="MTQGTVKWFNSEKGFGFISSETGTDVFAHFSEIKVDGFKTLEEG
                     QKVTFDIQDGQRGPQATNINLVK"
     gene            complement(52765..55341)
                     /gene="mutS"
                     /locus_tag="VBC52_000288"
     CDS             complement(52765..55341)
                     /gene="mutS"
                     /locus_tag="VBC52_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEO6881895.1"
                     /translation="MAKPTISPGMQQYLDIKENYPDAFLLFRMGDFYELFYDDAVKAA
                     QILEISLTSRNKNAEKPIPMAGVPYHSAQQYIDVLVELGYKVAIAEQMEDPKKAVGVV
                     KREVVQVVTPGTVVESTKPDSANNFLVAIDSQDQQTFGLAYMDVSTGEFQATLLTDFE
                     SVRSEILNLKAREIVVGYQLTDEKNHLLTKQMNLLLSYEDERLNDIHLIDEQLTDLEI
                     SAAEKLLQYVHRTQKRELSHLQKVVHYEIKDYLQMSYATKNSLDLLENARTSKKHGSL
                     YWLLDETKTAMGTRMLRTWIDRPLVSMNRIKERQDIIQVFLDYFFERNDLTESLKGVY
                     DIERLASRVSFGKANPKDLLQLGQTLSQIPRIKMILQSFNQPELDIVVNKIDTMPELE
                     SLINTAIAPEAQATITEGNIIKSGFDKQLDNYRTVMREGTGWIADIEAKERAASGIGT
                     LKIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGEVLEAREQS
                     SNLEYDIFMRVRAQVESYIKRLQELAKTIATVDVLQSLAVVAENHHYVRPKFNDEHQI
                     KIKNGRHATVEKVMGVQEYIPNSIYFDSQTDIQLITGPNMSGKSTYMRQLALTVIMAQ
                     MGGFVSADEVDLPVFDAIFTRIGAADDLISGQSTFMVEMMEANQAVKRASDKSLILFD
                     ELGRGTATYDGMALAQSIIEYIHDRVRAKTMFATHYHELTDLSEQLTRLVNVHVATLE
                     RDGEVTFLHKIESGPADKSYGIHVAKIAGLPIDLLDRATDILSQLEADAVQLIVSAPQ
                     EAVTADLNEELDSEKQQGQLSLFEEPSNAGRVIEELEAIDIMNLTPMQAMNAIFDLKK
                     LL"
     gene            complement(55398..55835)
                     /gene="argR"
                     /locus_tag="VBC52_000289"
     CDS             complement(55398..55835)
                     /gene="argR"
                     /locus_tag="VBC52_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO6881896.1"
                     /translation="MNKIERQKRIKRLIQSGQIGTQEEIKLHLKNEGIDVTQATLSRD
                     LREIGLLKLRSPEGKLYYSLSTATSNRFSPALRSYILKVSRASFMLVLNTNLGEASVL
                     ANFIDEKGLPEILGTMAGADTLLVICQNEDIAKVFEKELSVGL"
     gene            56057..57748
                     /locus_tag="VBC52_000290"
     CDS             56057..57748
                     /locus_tag="VBC52_000290"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEO6881897.1"
                     /translation="MDTKHLIASEIQKVVPDMEQSTILSLLETPKNSSMGDLAFPAFS
                     LAKTLRKAPQIIASDIAEQIKSDQFEKVEAVGPYVNFFLDKAAISSQVLKQVLSDGSA
                     YATQNIGEGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLANIFDKIGYHPVKINHLGD
                     WGKQFGMLIVAYKKWGNEEAVRAHPIDELLKLYVRINAEAETDPSVDEEAREWFRKLE
                     ANDPEATELWQWFRDESLLEFNRLYDKMNVTFDSYNGEAFYNDKMEEVLELLESKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSIYVVGNE
                     QSAHFKQLKAVLKEMDYDWSDDMTHVPFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKDKVAQAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKANFNPSNSDNYSLNDVESWEIIKLIQDFPRIIVRAADNFEPSIIA
                     KFAINLAQCFNKYYAHTRILDEDAEISSRLALCYATATVLKESLRLLGVDAPNEM"
     gene            57836..58144
                     /locus_tag="VBC52_000291"
     CDS             57836..58144
                     /locus_tag="VBC52_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO6881898.1"
                     /translation="MPSEKEILDALSKVYSEEVIQADDYFRQAIFELASQLEKEGMNS
                     LLATKIDSLINQYVLTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(58171..59043)
                     /locus_tag="VBC52_000292"
     CDS             complement(58171..59043)
                     /locus_tag="VBC52_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6881899.1"
                     /translation="MLKLDLKTKIKEAILIALGVALYTFGFVKFNMANHLAEGGISGV
                     TLIIHALFGVNPALSSLLLNIPLFILGARILGKKSLLLTIYGTVLMSFFMWFWQQIPV
                     TVPLKNDMMLVAVAAGILAGTGSGLVFRYGATTGGADIIGRIVEEKSGIKLGQTLLFI
                     DAIVLTSSLVYINLQQMLYTLVASFVFSQVLTNVENGGYTVRGMIIITKESESAAATI
                     LHEINRGVTFLRGQGAYSGREHDVLYVALNPSEVRDVKEIMADLDPDAFISVINVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(59151..60095)
                     /locus_tag="VBC52_000293"
     CDS             complement(59151..60095)
                     /locus_tag="VBC52_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6881900.1"
                     /translation="MKTRRTPLEKKVKYIISVWAKKFGLLHTLKSISREKYAEKISAS
                     LLYGILSSVAVNFFFQPGHVYSSGATGLAQVISAVSKHWFSFEIPVALAFYAINIPLL
                     ILSWRKIGHKFTIFTFITVTVSSIFIQLMPQITLTTDPLINAIFGGLIMGAGVGFSFK
                     SRISSGGTDIISLTIRKKTGRDVGSISFIINGIILLFAGLLFGWKYALYSMVTIFVSS
                     RVTDAIFTKQKKMQAMIVTSKPDCVIKRIHRDLHRGVTCINDAEGTYNHEKKAVLITI
                     LTREEFSDFKYLMLKADPKAFVSVAENVHIIGRFVDDD"
     gene            complement(60085..61836)
                     /gene="aspS"
                     /locus_tag="VBC52_000294"
     CDS             complement(60085..61836)
                     /gene="aspS"
                     /locus_tag="VBC52_000294"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEO6881901.1"
                     /translation="MKRSMYAGRVRSEHIGTSITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEEVSASVMATAESLRSEFVIEVSGVVTAREQANDNLPTGEVELKVQELSV
                     LNTSKTTPFEIKDGIEANDDTRMRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDNLEF
                     IDVETPMLTKSTPEGARDYLVPSRVNQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSDQEIQDIVEGMIAKVMKDTKGLEVSLPFPRMAY
                     DDAMNNYGSDKPDTRFDMLLQDLTEVVKEVDFKVFSEASVVKAIVVKNKADKYSRKNI
                     DKLTEIAKQYGAKGLAWLKYADNTISGPVAKFLTAIEGRLTEALQLENNDLILFVADS
                     LEVANETLGALRTRIAKELELIDYSKFNFLWIVDWPMFEWSEEEGRYMSAHHPFTLPT
                     AETAHELEGDLAKVRAVAYDIVLNGYELGGGSLRINQKDTQERMFKALGFSAESAQEQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SEQQLEELSLTVESYEN"
     gene            complement(61929..63209)
                     /locus_tag="VBC52_000295"
     CDS             complement(61929..63209)
                     /locus_tag="VBC52_000295"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEO6881902.1"
                     /translation="MKLQKPKGTQDILPGESAKWQYVENVIRNLFKQYHYDEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFHDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PTKMYYIGSMFRYERPQAGRLREFHQVGVECFGSNNPATDVETIAMGHHLFEDLGIKN
                     VKLHLNSLGSPESRQAYRQALIDYLTPIREQLSKDSQRRLNENPLRVLDSKEPEDKLA
                     VENAPSILDYLDESSQAHFDAVCHMLDALNIPYIIDTNMVRGLDYYNHTIFEFITEIE
                     DNELTICAGGRYDGLVSYFGGPETPAFGFGLGLERLLLILGKQGIPLPIENTIDLYIA
                     VLGSEANLAALDLAQSIRHQGFKVERDYLGRKIKAQFKSADTFNAKVIMTLGSSEVDS
                     REVSLKNNQTRQEVKVSFENIKTGFSSVLKQLGL"
     gene            63429..63611
                     /gene="rpmF"
                     /locus_tag="VBC52_000296"
     CDS             63429..63611
                     /gene="rpmF"
                     /locus_tag="VBC52_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEO6881903.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            63627..63776
                     /gene="rpmG"
                     /locus_tag="VBC52_000297"
     CDS             63627..63776
                     /gene="rpmG"
                     /locus_tag="VBC52_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6881904.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            64057..64683
                     /locus_tag="VBC52_000298"
     CDS             64057..64683
                     /locus_tag="VBC52_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEO6881905.1"
                     /translation="MRYFMIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIY
                     LGQFLGSVSLIFLSLLFAFVLNYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKD
                     GLRKDNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVTLLTFLVMIYLLVFS
                     AQKLAQVPSVGETLEKYSRWFIAVVYLGLGMYILIENNSFDMLWAVLG"
     gene            64695..65033
                     /gene="cadX"
                     /locus_tag="VBC52_000299"
     CDS             64695..65033
                     /gene="cadX"
                     /locus_tag="VBC52_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEO6881906.1"
                     /translation="MKKDSICQVDVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLAVEELCVCDIACLLNLSMASASHHLRKLANQNILDTRREGKIVYYFIKDE
                     EIRDFFNQLG"
BASE COUNT        20751 a        12921 c        10381 g        20990 t
ORIGIN      
        1 attatcaaga tacagaaaaa tctgttgata ttattaacaa ttagtcaaac tattacatat
       61 cggctatcaa aagttcctgt ctagacaaaa acacaaaaaa cgtatctaga aatagatgcg
      121 ttttttgtgt ttttattaat ctcccattcc tacacgttaa aatagtaatc tcaataaagc
      181 catagtaacg ataccagtaa gaacagtgta aaataaattt cgatatcgaa tagtgacaac
      241 tagagttggt atgccagcca ataaatctaa gcattttagt gttggcaatt gtcctaattt
      301 aactgaaaaa aagctagaga atgttagggc aaataaaata gaaagaggaa gaaattttaa
      361 aaatttcaca agactatttg gtagcccttt gtattttaca aatatgaatg gagcgactct
      421 tggcacccaa gaaacaacaa aacctaaaag aatagcaaga aaaacggtac tatttatcat
      481 ctaaaacaac tcccacacta catccaacaa cagtcgcaag caatactgat aaggctcctg
      541 acaaaaaagt ggctaataag aagtaactta aacctacaga agcaagaaca tatacaacaa
      601 gtcgctttcc atcagataac atcccaaaca actgaaatac aaagagacct ataaacatgg
      661 cgactaatgc aaagtctaaa ccaaacattt ctggattagg aatagtactc cccagtaaag
      721 ttcctattat cgttgagatt acccatgtca aataactcat cacattatta ccatgcatcc
      781 aactaggcga tacgactttg tgatgtaatg cttctccaag taaaacgccg tagctctcat
      841 ctgttactag agttcctatg gctaattgat tcattaggtg agcagacttg aagatagtag
      901 tcgcgtgtaa actcattaac atatttctca aattaactaa aaatactgtt atcgtaatag
      961 tcatcaagtc tgcttttgct aataatagcg cacacatagc aaactgagca gatcccccat
     1021 ataccaaggc actcataaga cccacttcta ttgcagataa atctgatgct gaagcaacta
     1081 tcccaaacgc caagcctatt gagatatacc ctaaagctgt gggcaaggca tctttcactc
     1141 catctttaaa tcctttttcc atcatatccc cctaaatttt cagtatattc agttattata
     1201 gcacaacggt gattactaag aaagaagggg aacgattatt tagctttgcc agcaattagc
     1261 ctaataaata tcctataatt attctataaa cctttgatat tgtagccata tagttggaca
     1321 atattattat tttcatagaa tgatttttaa aaaatacttt aggtacggtc ttaaattgac
     1381 cacaaaataa tttgtaatga aaattcagaa aattgtagaa aattttgatt ttttatgttt
     1441 taatagtttt tgtaattatt atgttacaag aaaaggagat ttttatgaac acaaaacagc
     1501 gtttttcaat ccggaaatat aagttaggtg ccgtatctgt acttttggga accctatttt
     1561 ttttaggcgg tatcacaaat gtagctgctg attctgtcat aaataagcca tctgatattg
     1621 cagttgaaca gcaagtaaaa gacagtccaa cgagcatagc aaatgagaca cctactaaca
     1681 acacgtcatc agcccttgcg tcgacagctc aagacaatct tgttacaaag gctaataata
     1741 atagtccaac agaaacacaa ccagtagctg agtctcactc tcaagccacc gagacatttt
     1801 ccccagccgc aaatcaaccg gttgaaagca ctcaagaagt ttctaaaact cctttaacca
     1861 aacaaaattt agcagtcaaa cctacaccag ctatttctaa agaaacccct caaaacattg
     1921 atagtaataa aattatcact gtccccaaag tatggaacac aggctacaaa ggagagggaa
     1981 ctgttgtagc aattattgac tcaggactag ataccaatca cgatgctctc caactaaatg
     2041 attcgacaaa agcaaaatac caaaacgaac agcaaatgaa tgctgctaaa gcaaaagctg
     2101 gtataaacta tggaaaatgg tataacaaca aagtaatctt tggtcacaac tatgttgatg
     2161 tcaatacaga gctaaaagag gtgaaaagca cttctcatgg tatgcacgta accagtatcg
     2221 caacagctaa tcctagcaag aaagatacaa atgaattaat ctatggtgtt gctcctgaag
     2281 cacaagtaat gtttatgaga gtcttctctg atgaaaaaag aggaactgga ccagcccttt
     2341 atgttaaagc tattgaagat gccgttaaac tcggtgctga tagcattaat ttaagtttag
     2401 gtggagctaa tggctcttta gttaatgccg atgaccgact tataaaagct ttagagatgg
     2461 ctagactcgc tggcgtttct gttgttatag cagcaggtaa cgacggtaca tttgggagtg
     2521 gagcatcaaa gccttctgct ctttatcctg attatggttt aattggtagt ccatcaacag
     2581 ctcgtgaggc catttctgta gcatcatata ataatacaac actggttaat aaagtcttta
     2641 acattatcgg attagaaaac aacaaaaatc tcaacaacgg attagctgct tatgcagatc
     2701 ctaaagttag tgataagacc tttgaagtag ggaagcaata tgattatgtt ttcgtaggaa
     2761 aaggaaacga caatgattat aaggacaaaa ctttaaatgg taaaatcgcc ttaattgaac
     2821 gtggagatat tacttttaca aaaaaagtcg tcaatgctat taatcacggt gctgtgggag
     2881 ctattatctt taataacaaa gctggggaag ctaacctaac aatgagttta gatcctgaag
     2941 caagtactat tcctgccatt tttacccaaa aagagtttgg agatgtttta gctcaaaaaa
     3001 gctataaaat tgtatttaac aatatcaaaa ataaacaagc taaccctaat gcaggtgtcc
     3061 tatctgactt ttcaagctgg ggattaacag cagacggaca attaaaacct gacttatctg
     3121 ctcctggagg ctctatttac gccgctatca atgataatga atatgatatg atgagtggga
     3181 caagtatggc ttctccccat gtcgctggtg ctactgctct agttaaacaa tacttattga
     3241 aagaacatcc agaacttaaa aaaggtgaca ttgaaagaac tgtcaaatac cttcttatga
     3301 gtactgctaa agcacaccta aacaaagata caggcgctta cacctcacca cgccaacagg
     3361 gagcaggtat tatcgatgtc gcagcagcag ttcagacagg attataccta actggtgggg
     3421 aaaacaacta tggtagcgtt acattaggaa atattaaaga taaaatttcc tttgatgtta
     3481 ctgttcataa tatcaataaa gttgcaaaag atttacacta tacaacctat ttgaatactg
     3541 atcaagttaa agatggcttt gtcacattgg ctcctcaaca acttggtaca tttacaggga
     3601 aaacgatacg gattgaacca gggcaaacta aaacgattac aattgatata gatgtttcga
     3661 aataccatga catgttaaaa aaagtaatgc caaacggcta tttcctagaa ggctacgtac
     3721 gttttacaga ccctgttgat ggtggggaag ttcttagtat tccttatgtt ggatttaagg
     3781 gagaattcca aaacttagaa gttttagaaa aatccattta taagcttgtt gctaacaaag
     3841 aaaagggatt ttatttccaa ccaaaacaaa caaacgaagt tcctggttca gaagattata
     3901 ctgccttaat gactacaagt tcagaaccta tctactcaac agacggtact agtcctatcc
     3961 aattgaaagc cttgggaagc tataagtcta tagatggaaa atggatctta caactagatc
     4021 aaaaaggcca gcctcatcta gccatttcac ctaatgatga ccaaaatcaa gatgccgttg
     4081 cagtgaaagg tgttttctta cgtaatttca ataatttaag agccaaagtc tatcgtgcag
     4141 atgatgttaa tttacaaaaa ccactatggg taagtgctcc ccaagcagga gataaaaatt
     4201 actacagcgg aaatactgaa aatccaaaat ctacattttt atatgacaca gaatggaaag
     4261 gaaccactac tgatggtatt cctttagaag atggaaaata caaatacgtt ttaacttatt
     4321 actctgatgt ccctggctct aagccacaac aaatggtgtt tgatatcact ttggatagac
     4381 aagctcctac actaacaaca gcaacttatg acaaagatag acgtatcttc aaagctcgtc
     4441 ctgcagtaga acacggggaa tctgatatct ttagagaaca agttttttac ttaaaaaaag
     4501 ataaagatgg tcattataat agcgtcttac gtcaaaaagg agaagacggt atccttgttg
     4561 aagataacaa agtatttatc aaacaagaaa aggatggtag ctttattcta cctaaagagg
     4621 ttaacgattt ctctcatgtc tactacactg ttgaagatta tgcaggcaat ctagtatcag
     4681 caaaactcga agatttgatc aatattggca ataaaaatgg tttagtaaac gtcaaagtgt
     4741 ttagccctga gcttaacagt aatgtcgata ttgatttctc ttactctgtc aaagatgaca
     4801 aaggtaatgt catcaaaaag caacatcacg ggaaagacct caatttactg aaattgcctt
     4861 ttggtaccta tacgtttgat ctattcttat acgatgagga acgagcaaat ctaatcagtc
     4921 ccaaaagtgt cactgtaact atttctgaaa aagatagcct taaagacgtc ttatttaaag
     4981 ttaacttact caagaaagca gccttactcg ttgaatttga caagctttta ccaaaaggag
     5041 caacagtcca gttggttact aagacaaata ctgttgttga tctaccaaaa gcaacttatt
     5101 ctcctactga ctatggtaaa aacatacctg taggagacta tcgtttaaac gtaactctgc
     5161 ctagtgggta tagcacttta gagaacttag atgatttact tgtatccgta aaagaaggtc
     5221 aagtaaacct aacaaaattg acgctgatta ataaagctcc tctgattaat gccctagcag
     5281 aacaaactga cattattacc caacctgtgt tttataatgc tggaactcac ttaaaaaata
     5341 attacctagc taatcttgaa aaggcacaaa ctttaattaa aaatagagtg gaacaaacaa
     5401 gtattgataa tgctattgct gctttgagag aaagtcgcca agctcttaac ggtaaagaaa
     5461 cagatacttc tttactggca aaagctattt tagctgaaac agaaatcaag ggaaactatc
     5521 aatttgttaa tgctagtcca ttaagccaat caacttatat caatcaagtc caattggcga
     5581 aaaaccttct acaaaaacct aacgtcactc aatcagaagt agacaaagcc ttagaaaatc
     5641 ttgatattgc taaaaatcaa ttaaatggtc atgaaactga ttactctggt ttacaccata
     5701 tgataattaa agcaaatgtt ctgaaacaaa catcatctaa atatcagaac gccagtcaat
     5761 ttgctaaaga aaattataat aaccttatca agaaagcaga attgctgctt tccaatagac
     5821 aagctacaca agctcaagtt gaagagttat taaaccaaat aaaagcaacc gaacaagagc
     5881 ttgatggtcg cgatagagtt tcttccgcag agaattatag tcaatcactc aatgataatg
     5941 actctctcaa taccacacct atcaatccgc caaatcagcc ccaggcgttg atattcaaaa
     6001 aaggcatgac taaagaaagt gaggttgctc agaagcgtgt cttaggggtg actagtcaaa
     6061 ccgataatca aaaggtaaag acaaacaagc ttcctaaaac aggcgaaagc actcctaaaa
     6121 taacctatac aatattgcta tttagtctct ctatgctagg tctggcaaca atcaaactaa
     6181 agtctatcaa aagagaataa aatactttaa aaaatagagc aaggcatcaa ttactagcta
     6241 ttaatagtta gtgacgggtg cctttttgag gagcttaaaa tgatgttcct aaagaccttt
     6301 tctctgcagt atcttgtttt ataacactat aacacttcaa agaataggaa aaaaactctt
     6361 tttttgttat atttgatatg acataaacag ctagctaaaa gtcaaattat aactatgggt
     6421 attgaggtat acatgacaaa aataatttat ttcgctgatg atgaaaagaa tattcgtgac
     6481 ttggtggttc cttttttaga acatgatggt ttcactgttc gagcatttga aactggagac
     6541 cttctcttag aagcctataa aaaccaaaaa cccgatttag ttatattaga cataatgatg
     6601 cctggaacta atggtttaga tgttatgaaa agcatccgcc aatatgataa tgttcctatt
     6661 atcatgttaa cagctagaga ttctgatgtt gatttcatta cggcttttaa tttaggaact
     6721 gacgattact tcaccaagcc tttttcacct ataaaactta gcctacatgt taaagcattg
     6781 ttcaaacgcc ttgatgaaaa agcaatcaaa aatgacactc agtatcagtt cctagattta
     6841 actctggata ctgaaaagcg aatagcccta ttatcaaatg aagaaatgcc cttaaccaag
     6901 acagaatttg attttctttt agtattaatt gaaaaacctg aaactgcttt ttctagggaa
     6961 acactgctta atcgtatttg gggatttgat gatattgaaa gcagagcagt tgatgacact
     7021 attaaacgat tacgtaaaaa atttaagcaa taccacagtc aagttagtat caaaactgtc
     7081 tggggttacg gcttcaaatt aatcaaagag gacaaatgaa aaagaaaagt ttaattagca
     7141 atcttatcct tattaatagc atcactctat ttcttggttt ttacctcatc tatttttctg
     7201 tcaactatgt cattaaagac tatgtccatc aaatgacaag gaattccatg caggcgaatt
     7261 tcaccatttt agatagtatc tataacaaca agaaaatacc agatgaaaat atggttaacc
     7321 aagatagcat ctttgtttgg tcttactatg ctatttttga ccagcattat cgtatgaaat
     7381 atggcactga taatcataaa aaagagagcc atgatattat tcgctatctt acccaaaaaa
     7441 gattgtggca aatttccaag gaaaaagatg gtatgtttgt aaccatcaag aaaaaaacct
     7501 attatgtaat gaccaaagac tactctggta tccttgttga tggtagcata aaaaaagttc
     7561 ctaaagcaca gtcgcaactt tttcatgtga ttaacttttc agacattact tacacccagc
     7621 atctaattac taaaattaat cattttctta tagttatctt agtactaacc tatattccca
     7681 tgctatttat tatgaggaag actttcactg gaattagaga atctattcaa tcagtacaaa
     7741 cttatatttc cagcctttgg aaaaatcaag gcaatcatca atctagtcaa aaagaaattg
     7801 tcttttctga tttcgatcct cttctacttg agagccaaga aatggcaaat cgaatctacc
     7861 aggcagaaga gagccaaaga aacttctttc aaaatgcctc ccacgagtta cggacacctt
     7921 taatgtccat ccaaggatat actgagggag ttcaagaggg tattattgat gccgagctcg
     7981 cacactctgt tattctgcaa gagagcaaaa aaatgaaaca gctcgtagac gatattatct
     8041 tactctctaa attagacagt aacttaagtg atcaaaaaga tgaattttct ttaaatgaat
     8101 tacttaatag tattattgct tatttcaaac ccctagctaa taaacaaaaa attagtatca
     8161 cttatagacc agacgagcat gaaaagttac taaaaggtaa cgaagagttg attcaacgag
     8221 ctatcaataa tatccttagt aacgcactac gctatgctgt ttctcatatt gaaatctctt
     8281 acacaaatca aaaactgact atctcaaacg acggaccagc catttcaaaa gaagacttac
     8341 cttatatttt cgatcgcttc tataaaggac atggcggcca aacaggtatt ggacttgcaa
     8401 tgactaaaga aattatcaag caacatcatg gtaatatcat cgctgagagt gattcaacct
     8461 caacaacttt tacgatttat ttcaattgcc acaaaattgc ctaacttttc ctacaaaaat
     8521 tcaaagaagt tattgctttt ttaacctata atacctatag atcaaaaagt tattctaaaa
     8581 gaaagtgagt ttttatatgt tacattctaa aaaaatacat tccttatcgc ttattgccgt
     8641 tctctcttta gcaacatata cgagtttaca accaaatcat gtaacggctg aacaatcaca
     8701 aaaaacatca actgttcata tgagtcaaaa aactattgaa cataagttaa aagttgcaga
     8761 taaagaagct gctcctctct acgctaaaat cgaccatatc caacgacata ttgaagtcaa
     8821 aaaagcaaaa gatttaaaag ttattgaatt gtatattaac aaagatatca accaactaga
     8881 gaagcaaaat aaacgtctac taactaaatt ctatacttct attgataatc aaacatggga
     8941 tagcacaagt gaggtcaaaa aattgattga taagacaaac ctatccacta acgaaaaaaa
     9001 tagattaaaa ttatattttg aacaacgtgc ttaccttgag acaaggttga acgaccgcta
     9061 tcaaaaattt gataactcta ttgaaaacca aaataaagaa ctaaaaatat taacgtcaaa
     9121 aatcgaaaaa atctatcaaa aacatggtat tacaaaagag gtattaaaaa cttactatgc
     9181 taaaaaaaca gtacgagctg actgatttag ctaaaaatag aaaaatccca aaagttcata
     9241 aacttttggg attttatacg cgtaaatatc actatttcta gtaaaatctt tatcttattt
     9301 caccacaata ttaaccagtt tgtttggtac tgcgatgact ttgatgatgt ctttgccagc
     9361 gatttcggat tggacttttt cgttagcaag ggcaagatct tgtaattctt cgcgtgaaag
     9421 gtctttagca actacaagtt tagctttgac cttacctttg atttggacaa cgatttcaat
     9481 ttcgttttca accaatttac tttcgtcata gcttggccat ggcacgtaag agattgactc
     9541 acctgaggct gtcaagactt gccagagctc ctcacctaag tgtggtgcga atggtgcaat
     9601 caattgaaca aagcctttgg catagtcgct aaagagtttg tcttccttgt tagctgcatt
     9661 gacaaagacc atgagctgag cgatagctgt gttgaatttc atttggtcaa cttgctcagt
     9721 aacagcctta acggtttcgt tgtaaacctt gtcaagggct ccgctatttt ccttagtaat
     9781 ctctttagtc gtgatcaaac ggtaaacacg gtccaagaac ttacgtgagc cttcaagacc
     9841 ttcctcagac caagcgattg aagcgtcaag tggtcccatg aacatctcgt aaacacgaag
     9901 ggtgtcagca ccgtactgtt caacaacatc atctgggttg acaacgttct taagtgattt
     9961 agacatctta gctggtgctt gttcaagctc ttccccagtt tccacatgga agaagctacc
    10021 atcacgtttt tcaaccttgt cagttgccac aagtgcacca cggctgtcgc ggtagcttgt
    10081 tcccaaaatc atcccttggt taaagagttt ttggaatggc tctttggtag gtacgacacc
    10141 gagatcgtaa agaaccttgt gccagaagcg agcgtagaga aggtgaagca cggcatgctc
    10201 tgcaccaccg acatagatat caactggcaa ccattgtttg agaagttcct catcagccag
    10261 tttttcggtg ttgtgtggat caatgtaacg gagatagtac cagcttgaac ctgcccattg
    10321 tggcatggtg ttagtctcac gacgaccttt aacaccatct tcacgagtca cttccaacca
    10381 gtctgtcaag ttagcaagtg ggctctcacc agtacctgaa ggacggatat ccttggttac
    10441 tggaagaacc agtggcaatt cactctcagg aaccgctgtt gatgtcccat cttcccagtg
    10501 gatgattggg attggctcac cccagtaacg ttgacggctg aagagccagt cgcggagacg
    10561 gtaagtgact ttctcattac caacaccttc tgcttcaagc cattcgacca ttttggcaat
    10621 agctgacgct ttatcgagac catctaggaa gtctgagttg atgtgaagac cgtcttctgt
    10681 aaaggcagcc tcttcaacat taccaccttc aagaactgga atgatgtcta gcttaaattg
    10741 ttttgcgaat tcccagtcac gttcatcgtg ggctggaaca gccatgatag cacctgtacc
    10801 gtagctggca aggacatagt ccgcaatcca aactggaatt tctttaccat taactgggtt
    10861 aatagcatag gcacctgtcc agacacctgt tttttcttta gcaaggtctg tacgggcaag
    10921 atctgattta agacttgctt gacgtttgta ttcggcaaca gcttctgctt ggtcagctgt
    10981 tgtaatagca tcaaccaagg catgttcagg agcaagaact gcgtaagttg caccaaaaag
    11041 ggtgtcagga cgtgttgtga agacagtgaa gtccttatct gtatccttaa ccttgaaggt
    11101 tacattggca ccagttgatt taccaatcca gttgcgttgc atgtccttaa ttgattctgg
    11161 ccagtcaact tcttccaagt cttctagcaa gcgctctgca taggcagtga ttttgagcat
    11221 ccattggcgc attggttttc tgacaactgg gtagccgcca cgttctgatg tgccatctgg
    11281 aagaacctct tcgttggcga tggccgttcc caattcttca acccagttaa cgggaacttc
    11341 agcttcatag gctaggcctt tttcataaag cttagtaaag atccactgtg tccacttgta
    11401 gtagttaggg tcagttgtgt tgacttcgcg atcccagtcg tatgagaagc caagagcgtt
    11461 aatctggcgc ttaaagttag cgatgttttc agctgtaaac tcggctgggt cattaccagt
    11521 atccatagcg tactgctcag caggaagtcc aaaggcatcc caacccattg ggtggaggac
    11581 attgtgacct tgggcacgtt tgaaacggct gagaatatca gttgctgtat agccttctgg
    11641 gtgaccaacg tggagaccag ctccagatgg gtatgggaac atatctaggg cataaaactt
    11701 aggttttgat gcatctgttc ctgttttaaa agtatgatta tctgcccaaa aggcttgcca
    11761 tttgggttcg atttctttgt gattatagaa tgtcatcgca cttctccatg tcaaaaaagt
    11821 atagtcttct tattataaca cgaaaaatag caataaaaaa ggtgaaaata ccctcaacag
    11881 caggttctct ccccacttat ccttgacaat aaactaaact tactctatac tatttccaaa
    11941 tgacataatg ctttaaccgt gtcctgtgag acatataagc gaccaaaata ggattctata
    12001 cagaaatcct actatttttg cgagcgttta tagaactcaa gggtagcatc ttgagttttt
    12061 tcttttacat tataatgagt tgaagcagct atgacatctc tgttttagaa ttttttctat
    12121 tgacagtatt taataacggt ggtctgtgag ccatcattaa aggaggagac ttatgtcacc
    12181 attaaatatc aataagaata attggcgcgc cctaatcgct gccattgtcg catccgggac
    12241 cgatgattta aatatcatgt tccttgcatt ttctatgtca actatcatta ctgatcttca
    12301 tttatctgcg gcacaggctg gttggattgg gacaattacc aacttgggaa tgctcgtggg
    12361 aggactaata tttggtttat tagctgatcg ctacaataaa tttaaagttt tcaaatggac
    12421 gattctaatc ttttcaatcg caactggttt agttttcttc actactaatt tatcatatct
    12481 ctatatcatg cgcttcattg caggaattgg tgttggtggc gaatatggta tcgctattgc
    12541 cattatggca ggaattgtcc cagctaataa aatgggccgg atttcttcac taaatggtat
    12601 agctggtcaa gttggatcta ttagctcagc actcttagca ggatggctag ccccagcact
    12661 cggttggcgg ggactttttc tctttggcct tctccctata gttctcgtcc tatggatgca
    12721 atttgctatt gatgataaag atatcttaga tcaatataat acagacgcag atgatgaacc
    12781 tctagatatc agtataaaag ctttatttga tactcctgta ttagccacac agagcctagc
    12841 actaatggtt atgacaactg ttcagattgc cggattcttt ggcatgatga actggttacc
    12901 aactattatc caaaccaact taaacgtttc tgtcaagaat tcatcattat ggatgattgc
    12961 aaccattctt ggaatgtgcc ttggcatgct agtatttggt caattgttag ataaatttgg
    13021 accgcgttta gtatacgggt gtttccttct atcatctgca atttgtgtct atctctttca
    13081 atttgcaaca acaatgcctt ctatgattat aggcggggca gttgtcggat tctttgttaa
    13141 tggtatgttt gcaggctatg gtgccatgat tacacgtctc tatccacatc atattcgttc
    13201 aacagctaac aatcttatct taaatgttgg tcgtgcaata ggtggctttt cgtctgttat
    13261 catcggaatg attctagacg tttcaaatgt ctctatggtc atgcttttct tagcaagtct
    13321 ctatatcgtt agtttcttat caatgctaag cattaagcaa ttaaaacatc aaaaatatca
    13381 cactaattta acacaattag atgtcaaacc aactgactga tattacaaaa cagtattcct
    13441 caattgagaa atactgtttt ctttatttca ttagataact attcagctca ggcatcccac
    13501 ttttagcatc tttcatacca agctgataaa tactatcaag tttatccgga ttcttctcta
    13561 agcggccaat aaccaagctc ttacttggtc taattgcaaa tagatcgcct gttttttcaa
    13621 ggctcatgac cttttcaagg ctattattat actgttggta ccggttcgag gctgtcttta
    13681 caaaattagg atatttccta tacagagttt tatacaatcg tccacttgaa ggctttttct
    13741 gataattgag cggcctagtc atcacaacaa tcaacttgtc aaatcctaaa ccacgggcaa
    13801 aatcaacggg aatactatca gataaaccac catctaagta ctttttcccc tgccaaacaa
    13861 ccatctttga gactactggt aatgctgaac tagcacgtaa aatttccatt tgttcaaaaa
    13921 cactatcaat tttaaaatac tcaggtttac cagatgtcat ctctgtagca actgcgtaaa
    13981 aatcaatact tgatttttta aatgcttcat cgtcaaatac atccaatttc ataggaactt
    14041 cataataggt gaaatcttta ttaacaaaat tccctgttcg aagccatgac cttagactca
    14101 tatatttagg gtgggataaa tactttttat tgtatcgcaa agccctctct cgttgtctag
    14161 atacaaaatt aacaccaaac aatgcaccag cagagacaga tatgatacca tctactttta
    14221 ttcctgcatc tagaaaagca tctaaaactc cagcagtata aagacctctc attccgccac
    14281 cctctaaaac taaaccaaca gacaacatag gcaatcactt tctaatattg atatttgatt
    14341 aaagtatagc aaaatatagc tattttttct gaaaaagaat ataaagttta gatcatgtta
    14401 actaaaaagc catgataaac aaccgtctaa catagccttt ttgttttata attctgcaac
    14461 ttggtacaac tcaagttctg cttgtgtttc tgagccgaac atgttgatca ttaatttaac
    14521 tttgttattc tcaatctcaa ctacacgtcc ttcttgtccg atgaaagcac catcaatgat
    14581 ttgaacaaca tcaccttcct tgatattagt atcaaagaca tcaacggttt gtcccattga
    14641 gatgagtatt gaacgaatct cttcttctaa caacggtgtt ggtttagagc gattgccgtg
    14701 tgatccaaca aatccagtaa cgtttggagt attacgaaca acaaaccagg cttcatcagt
    14761 cattaccatt tccacaagta cataacctgg gaagcgattt tcttcaattt cttttgtttt
    14821 cccatttttc tcaacattta cagtttgagt agggatttcc acacgcaaaa tgttgtctaa
    14881 catattataa gtttgcgcac gttgcaagag attttccttt actttatttt cataacctga
    14941 gtaggtttgg agtacaaacc aacccttatc aaaagaatct aacataagtt ttcctttcct
    15001 taataaaaaa gccttttacg gcttattgga ctgctatttt caatatatat tataaagaaa
    15061 ataaaataag ctgtcaatta aagtaccgac taaatcattc taaaattcaa ataaaaaagg
    15121 caaagttctg atgaggtttg ccttaagatt ttgaaaggat agacctttcg gtttatgttt
    15181 aaaaataaat ttaatttttt ttatgcttta aaactatcgt aataagacct gcacttaaca
    15241 taacaattag tgatacaatt gctaagagtg gacttgcggc ttcacccgtt gatggtaatt
    15301 ttttactata ctttttgttt tcaatagctt ttttaaccgc caagtttccg ctagtattaa
    15361 ccgatttttt ggttgctggt ttggcttctg gtttagcctc tggcttaacg tctggtttaa
    15421 cttctggctt agcctctggt ttggcctctg gcttaacgtc tggtttaact tctggcttag
    15481 cctctggtct ggcctctggc ttaacgtctg gtttaacttc tggcttagcc tctggtttaa
    15541 cttctggctt aacgtctggc ttagcttctg gcttaacgtc tggtttggcc tctggcttaa
    15601 cgtctggttt aacttctggc ttaacgtctg gtttggcttc tggcttaacg tctggtttgg
    15661 cctctggctt aacgtctggt ttaacttctg gcttagcctc tggtttggct tctggtttgg
    15721 cctctggttt ggcttctggt ttggcctctg gtttggcttc tggcttagct tctggcttaa
    15781 cgtctggctt aacatctggt ttggcttctg gtttaacttc tggcttagct tctggtttgg
    15841 ctactttttt atcttttaat tctaaacttt gtaaagcatc acgtagcttg ttagctaatt
    15901 gatttgcttg atcagtggta ataaattttc cctcttccac atgatcataa gcttggtcaa
    15961 cagctttttg aactgcaagt atatctacat atttgtcctt tacttgatca tctttaagca
    16021 gatctttagc ttgattatac catgtagtca atcttgtcaa ggcttctggt gacaaagctg
    16081 gttttgcagc ttctgtaggt gcttgtttag gtacttgaat atttggtttg gcctctggtt
    16141 tggcttctgg cttaacgtct ggttttttat cttctttagt acctctggta aggtcttgaa
    16201 gttcttttaa aagctttttg acttcatcaa gcattttttt tgcttcatca agtccacttt
    16261 gaccagtcca aacacttcta tttccgaaag ttcgtttatc tctaaaccta ttcaaataag
    16321 ttcccacaga gtctgcataa tatccaggtg taggcaaaat acttttatgc ttttcataaa
    16381 tttctttagc ctttttggat aacgctaatc cttttaaact tattctttca atttttttag
    16441 catactgctt ttgtaagttt ttctgggcaa cttcctgtgc tgcttgctta atactattta
    16501 aagcattcat tgcatcagta atattgagct taccttcatt atactgtttc actacattat
    16561 caattgcagt attatatctc aacatttcat cgttagataa attttctaac ttagcaattt
    16621 cttgcttttt accattggcc tgatttacca atgtttcttt attggaatta acaacttttt
    16681 tagcttctgc tacaagctca tcaacttgac tcttagtgct agctctttga attttttctt
    16741 tatattcttc taagacttcg aggcttatat tttttattga agccacgtca ttttgagcca
    16801 tgtgcttagc ttttgttgat tcagcattat tcagttgcgt ggcttgtgtt acaaatttaa
    16861 gaataagttg cgaatctttt gaagagctaa tagatttaac taaaatatct ttttggtctg
    16921 atgaaaggaa acctaatgat ttaatgatat cttgagaacg tcttgactgc ttagctagtt
    16981 cttgatcact aactttaaca tgtgtagcac tagtactttt tattgattga ggtttaattt
    17041 tatgaattga ttcagcctta attgtattaa tagactcggc tttgattgtg ttaattggct
    17101 tagcttgaat tttattaatt gattcggctt tgattgtatt aatagactct actttaattt
    17161 tattaatgga ctcagcctta attgtattag ttgatttacc ctgaatttta ttaattgatt
    17221 cagctttaat attattaatc gactcagcct taattgcacc acttcccaat ctctcaactt
    17281 ttaatcttac gactggtata ctctgtttaa taattcgtcc ctgaggtaaa gtattgttaa
    17341 tttctgcttc agtactatta attaattgtg gaagtgtatc ctgtgatgct ctattaaaat
    17401 catctactct acttagaagt atagcaatag ctttgtcatt accacgatat ttagaaatca
    17461 aattcttcag agtaaccgcc cctaaattaa cttgtttctt atgaggaatt gaatccgata
    17521 ttcctgaact agtatccgcg tgtacaatac cactacatac agcaaacgct gctgacattg
    17581 aggccaaacc ataagctgtt tttcttaaaa agtattttac ttttttttcg ttattattca
    17641 tattcgctcc tttatatagt tagttaaaaa tcctaattta ttaggatagg accacgttta
    17701 ttttaattta atttgagttt cttaactaat tatatatctt gtagcttttt atttcaacac
    17761 ttttctttaa tttatttaca ttttattaaa ataaatattt ttttttaata tttttttgct
    17821 caacaatcta gtaaaaacaa tcaaaagatt atttttttat aatctagtcg gatatatatg
    17881 ttattttcca cacaaaaaac ccttagtaaa atctaagggt ttgggttgtt tctatctaaa
    17941 ataatctatt attaaaatcg attaatcaag tccatgactc ctgcggctaa cagtttgtca
    18001 aagatgtaga taacgattgt gaaaaataca gtgtattcca agatggaaat aaaatctttc
    18061 caacgttgtt ttcgatttgg ccaagtagtg tcacgcaata cttgaaaaat ccctttgaca
    18121 aacattatca gttctccttt ttgcagtttg ttacgctata tcaacgcgtt tctttgtgta
    18181 ttgtatattt cttacaatat ttacaaaatt tatttacttc tagtcgtgtt ggcttggggg
    18241 tgctacttac tccgattgag taatttcggc ttccgcattc tgtacacgct aggcttgctt
    18301 tcttttgtgc cataacgcct ccataaatgc tattttaaca tgatttgtat aatcctgcaa
    18361 gctatctaaa gtagtccttt agactattcc aagcatcttt taccttctct tggaaatttg
    18421 aatcttccac cgctctagaa atatcttgtg cagccttact tgccgaatct tttaaatcat
    18481 taataattga gcgtgattct tggttcgttg tattactagc atcttgtgca ttataaactg
    18541 aaccaatacc gttttggaaa taagcattct caatatgagt aaaactagac cccttagtat
    18601 acggtagtat atatgaagct tgagtgctaa atatatttga agctgtacct gcactagaat
    18661 ctgtcaaata atggtgttta tctgtcttct taaacccaac ccattgacta ataacgacat
    18721 caggcgtata gccgactacc cactggtctc cagacaaatt ggggttaaaa tctgtttcgg
    18781 ttgttccagt tttacctgcc attgtataac cataaacatt ggcattaata gctgtaccat
    18841 ttgagaatgt tcctaacatc atactagtca ttttactagc cactgagcga ctaataactc
    18901 gcttaggctt atctgtaaat tgctttacta attttccatt agctgtctca attcgagtaa
    18961 tcaaatgagc gcgatgcatt ataccgtcat ttgcaaaagt tgagtaagct tgtgccattt
    19021 ctaaaggatt agtggttaca cttccaccta gcgccactcc tagctctttg tttgctgatg
    19081 acatatttag accaaacttc ctgccataag taaaggcttt attgattcct agtttgtcta
    19141 aagtataaac agcaggaata ttataagaat tagctagtgc ttgatacatc gggatacttt
    19201 cagtctcaat cccaccatag ttacttggtt tataaccatg aaaatcttga actttattag
    19261 gaagttcttt atctattgac catcctgaag caatagctgg tgaataaaca actaaaggtt
    19321 ttatggttga ggctggactt ctctttgatt gagtcgcgta gttaaaactt ctaaactgtg
    19381 catctttagt actttgaaca cgtccaacta aacctcttac accaccagtt tttggatcta
    19441 aagctacgct agcaccttgg gcagattgac catcatagtc agatacaggg aagaggctag
    19501 tatcatcaaa tgttttttgc attcctgatt gataattttg atctaatgca gtgtaaatct
    19561 tatagccatt attgacaata tctttttctg aaataccata agtatcaatg gcttcattga
    19621 caacagcatc aaaataggat gggtacctgt aatcattaat tttaccagca taggtatctg
    19681 ctaatctatt tttcatacca attgaagcag cttctttagc ttgagacttg gttagtttac
    19741 ctgcatcaac catcgctgcc aaaacagtat ctcttcggtt agttgcattt tcaacagaat
    19801 aataaggatt ataaacttct ggccctttaa gcattccagc aagtgtagca gcctcatcta
    19861 ctgtcaaatt agcagcactg gttccaaaat atttcctact agcatcttcc acaccccaaa
    19921 caccattacc aaaataagag ttattaagat acatggtcag aatctcagcc ttgctatatt
    19981 tcttagtcaa ttctagcgct aaaaagaatt cacgcgcctt gcgtttaatc gtttggtcct
    20041 gtgacaagta agcatttttt gctaattgct gggtaatagt tgagcctccg ccaaatttcc
    20101 caagtgtagc tactgccaaa aaaaatcgct taaagtttac accattattt tcataaaaag
    20161 ttctatcttc agttgctata acagcatttt tgagatgatc agaaatagca tttaactcta
    20221 cataagttcc cttttgtccc gaaagttttc cagcgtattc tttattctta tcataaatgg
    20281 tggtagttgt ttttagagca ctctgtaaat cactaactgt agcagtttta gaaagataaa
    20341 aaagatagct accaaatata agaataaaag ccattaaggc gataaaaagc aacttcccaa
    20401 tacgatagcg ccgccaaaaa cgacggatag gattcttagg agaagggagg taacgatcaa
    20461 ctttttgtag ccattcaggt cgagatttag catttttctt ttggtgacga atgcctcgct
    20521 gatactggga tttactaggt cgggtaatat catcaatatc atagacttct tcgccatcca
    20581 aattactagt ttcttctcta gtttcttgtt cattttttct tgtcatttcg tcgttgtctt
    20641 cgtcaacacg aaataagtct ataaacttat caaataattt catagactta ttatatcaat
    20701 tttcaataaa atgctataat aaaaccatgt cattttcatt aaaaattaga aatccatacg
    20761 gtgaacatac cgttaaagaa ctccttgaag attatttttt gattccacgt aagattagac
    20821 attttttgcg tgttaaaaaa catgtactta taaacaatga attcattaat tggcaaactg
    20881 tcgtccaaga aaacgatact attaccttaa tctttgatga tgaggattac cctactaaaa
    20941 aaattcctct gggcagagca gagcttattg attgtcttta tgaggatgaa catcttatta
    21001 tcgttaataa acctgaaggt atgaaaactc acggtaacca accaaatgaa atagcactgt
    21061 taaatcatgt atctgcctat tctggacaaa catgctatgt tgttcatcgc ctagatatgg
    21121 agaccagtgg agctgtttta tttgctaaaa atccatttat acttcccctt atcaatcaac
    21181 gcttagaacg aaaagaaatt tggcgtgaat attgggcttt agttgaagga aaattttcac
    21241 ctaagcatca agtcttgaga gacaaaattg gacggaaccg tcatgacaga cgtaaacgaa
    21301 tcattgattc taaaaacggt caacatgcta tgacaatcat tgacgttttg aagtataccc
    21361 aaaatagtag tctcataaaa tgccgactgg aaaccggaag aacccatcaa attcgcattc
    21421 acttatctca tcacggacat cctttaatag gagatcccct ctacaaccct tcttctaata
    21481 atgaaaggtt aatgctacac gctcaccgat tgactctatc tcatccacta acttgcgaaa
    21541 ctattagcgt agaggcccct tcatctactt tcgagaagat tttaaacaat tataaaaaag
    21601 gagttggata ataattatcc aactcctttt aatcattatt gcttagttaa tggcttactg
    21661 agtagtggtt tatggtaatc aaattctcca tcactagatg tctgccagat tactgggatg
    21721 atcttattcc cttcaatttt tattccatag gcgtatttaa ctttaagacc accgatacct
    21781 cctgttacaa aagttgtagt atcagtacct gattcatatt gataaaggta gagcccattt
    21841 cccttatcct ccaacttcgt aactttagca gaatggttac tttccttaga tttactatcc
    21901 ttgtaaactt ttttctgagt aactttacca tccttatcaa aagtcatcgt tactgtaccg
    21961 tcctcaactt cacctttcca acttcctaca aggttctctg gaaaaatgga aacgggatca
    22021 gccttaactt tattagtttc cttagctact gtgatatctt ttttaccatc attagaaata
    22081 aggtaactat ttcctgattt tttcataata aaattaccag ttaaattctg aataatgtgg
    22141 ccatatgttt tcttatcagg atctgttgat ttatcataat taaaatcata atcggcagca
    22201 aaattaactt gataactttc tttcccaacc tgtgtcattt tattaaggac aatattagga
    22261 atagtgaaat ggctcgcttt acgattatca gttacaaatt ttgctttaat actttctttc
    22321 aatcctttgt aaaaatcatt ttcggcacct ttttcaaaaa ctgttcctaa agtctgaggg
    22381 tcttgcccag ttgatacata aagtatcaat tgattgaaag cagttatcaa tttctgtcca
    22441 gcttcttttt catttaataa accatcaaca tctaatttga ttgtctgatt atcagcgata
    22501 gagattaatt tttgtttatg agaggttatc tcaccatcat taaaaacctt tttgatataa
    22561 gcttttgaac catcagtcac tggataattc tctacggaat agctaccatc ttttaattta
    22621 gcaatacgat tatcaccaaa ataaagatta ccatccataa gattacttgt tactgtaaaa
    22681 gatttaaaag cgaccgataa atcaatagtt tggtttttac catcataatc ttttttgagt
    22741 ttaatctctt ttccgtcact agtcccttca aggctagcag tataatgggt tctaggtaaa
    22801 cgttccaccg ttactgaaaa atgatctgaa ttgcttgttg cgaccttttt ttgattcaac
    22861 aaaacatcca atttaggaac attggttttc aaggtcaaag acataggctt attagcaata
    22921 cgataatcag ggaatatacc taagcgacta cctacacgct ttagatacat tgaatcattt
    22981 ttgtttcctt cttttatttc ctgccctaat ttacgcgcct cacttttaga caagggctca
    23041 aaattagcgt aattggcctc atcatttgtg atagattctc ccgtatctga ccaaaccatg
    23101 tattctttaa tattatcaaa cgcttgacct gacgtacgag aacgtgctgc tacaaaacga
    23161 ctaagtactt gtccctttga aaaataaaag aatcctagac tcagaagtga tactaaaact
    23221 ataccaaaaa taactaagca cactttaaca acttttttct taaaaaaatc aaacatacct
    23281 tatcctctca ttcactttat attaaagtaa attgcttaaa gaaaagttac aacactgata
    23341 aaacagctgt atttgtactt gtaaccgata tttttctatt ataacaaaac gaaaaaagga
    23401 ttgaaaccat aatccgattt caacctctaa aatttttgtc tatgattata ccaagtcctg
    23461 caagaagctc tctccaatca tcgcagtatc aacaccaaag ttatccgcaa ctgttgctga
    23521 aatatctgcg aaatgcccta ctgggataag tccgtttcct gtgaaggatg ggctataggc
    23581 taagagagga atgtattctc ttgtatggtc tgtgcctgca taagttgggt cattaccatg
    23641 atcggcagtg atcaaaagta agtctttgtc acgcattgct gaaatgatct ctggaagacg
    23701 ctcatcaaac tcatgaagac aatcacgata gccatgagga tcacgacggt gaccatagag
    23761 agcatcaaag tcaaccaaat tagtaaagga gaagcctttt tcaaactctg ataaccccat
    23821 tgtttttatc aaagtatcaa taccatgact atttgatttg ttatgtccca tatcatgatt
    23881 aatgcctgaa ccattaaaaa tatcattgat tttaccaaca gcataagtat cgatgccagc
    23941 ttgatctagt ttatttaata cggtatcttc aaatggtgat actgcatagt cgtgacgatt
    24001 ggctgtacgc gtaaagttgc ctggttcacc tacatatgga cgagcgataa tacgaccaag
    24061 gagagctgga cgctccattg tgatactacg agcatattca caaatacggt agagttcttc
    24121 tagagggatg atatcctcat gagctgcaat ttgtaggact ggatcagctg aggtatagat
    24181 gatgagctca ccagtttcca tttggcgtgg tccgaagtca tcaataacag ctgtccctga
    24241 ataaggttta ttcgcttcac gaataacttt acgtcctgaa aaatcctcaa tctttgtgat
    24301 gatatcttct ggaaaaccat tccagaaagt atcaaatggc tctgtaatat ttaagcccat
    24361 gatctcccaa tgccctgtca ttgtatcttt accaagtgat acttcttgta acttcgtcgc
    24421 gtagccactt ggattttctt ctgcagggac ggtcttaagc gcttggggac gaggaatatt
    24481 accaagtccg attttagcca tgtttggcac cgcaagtcca actgtttttg aaatgtggcc
    24541 aagcgtgtct gaagcaccat caggaactcc tgcattgaca aaatcattgg catctggtgc
    24601 cgctccaata cccactgaat ctaagacaac taagtgaatg cgatcaaatt gtgacataac
    24661 ttctccttta tcaattgaaa ataggaagtc agaccagcat ccttcctatt tcctttatat
    24721 tatttaataa gtgccttctt caactttttg gttcttaaca atcttgatga tacggctagt
    24781 acccaagcgt gaagctccta aagcaataaa tttttcagca tcttccaatg atgagattcc
    24841 acctgctgcc ttaattttaa caccttcgcc gacatatttt gccatcactt caacatcttc
    24901 aaatgtagca cctgctgtcg aaaaaccagt agaggtttta ataaagtctg cacctgaacg
    24961 tgtgacaact ccacaaagtt cgataagttc ttctttagtt aattgacatg tctcaacgat
    25021 aacttttaag atatggtctt gacatgcagc cttgatttga cgaatttctt cttcaacagt
    25081 atcaaaatcc ccatttttaa cgtctgtcaa attaatgacc atgtcaattt catcagcacc
    25141 atttttaata gcatcttgac attcaaaaac cttcgctgca gttgtactat agccatttgg
    25201 gaacccaata acagtacaaa tagctaattt acctgaaacg tactctgctg cttttttgac
    25261 gtaagaggct ggaatacatg ctgaagctgt ttcataagcc attgcatcat ctaaaattgt
    25321 ttggatttct ggccatgttg ctgttgttgc tagcaaagta tggtctaccg tttttaaaat
    25381 atcttttact tccacgttac tatccttctt tcttttgtca acttttagaa tagagttaca
    25441 ataccgacga taaaggcgct aagcatactt acagcaatac caccaatcat tgcacggaaa
    25501 acaagtcgag ctagggtgcc acgtttctct ggacaaagaa cagcaatacc tgaaacacaa
    25561 atacctaaac ttgataaatt agcaaaacca caaagtgaaa tagttgctac caatgctgta
    25621 cgataatcta aagatttgat taggtgaccc aattgttgga acgaaacaaa ctcatttaaa
    25681 atcaacttac ttccaagaag gtttccttct agaagaatgt ttttgtggtc aaatcccata
    25741 agaaaaccaa atggagcaaa aacatatgag aagatttgtt ctaagcggat tcccaatcca
    25801 cttaacatca tattaatcaa agaaactaaa ccaacaaagg caatcaaact agcaccaatt
    25861 gagaaagcca tttgtgcacc tgtgcttgca ccctcagcga ttgcatcaat cacattggcg
    25921 ttattacctt tattatccat cttaatgtca tcaatttttt gaacaggttc tgtttgaggc
    25981 aataagattt tagcaatgag aatactgcca ataggaacca ttgttgaagc aatcaagaga
    26041 tattccattg gaatgcctaa tgcaatgtag ccaccaagaa tagaaactga catacttccc
    26101 atacctgata ccaacacaac cattatctca ctatcagtca tacgacctag gtatttgcta
    26161 accaaaattg gactgtctgt ttgaccaaga aacatattag ctacggcaac aaaactctca
    26221 acctctgagg atttcataat tttaccaaca cccttaccta tccattttac tacaaatcca
    26281 aggattccta cataataaag tagactaact agggcagata agaaaacaat attaccaagc
    26341 gtttgaatgg cgaaaataaa accagttttt gctccactat ctgctaatga accaaacaca
    26401 aaatttaaac cagcttgacc acagttgatt actttagtaa ctccagttga aacaacacta
    26461 acaacttgtt ggcctagtgg gatacgtact aagattagcg caataatgaa ttgaacgata
    26521 agagcttttc caattaaact tagagaaaca ctcttacgat tgaaagaaat tgcatacaca
    26581 attcctaata ccaataaaat accaataata ctataaataa attgcatgat gacttccaaa
    26641 acctcgctta ttgtgattta tcattttcaa taagtgtgcg gagtgcttca acagcaactt
    26701 gaatagctgc ttctgtgtca tgagccattg ggttatccat acccaaagcg ttgcgttctt
    26761 gattaccaac aacaaggaaa tctgatccac aacgaactcc tagatgactt gctgctacaa
    26821 aaagagctgc agattccatt tcagacgctt ttgttcccag acgtttccat gcttcccatt
    26881 tatttaataa ttcataacta actggcatac gttctggctc atgttgtcca taaaatgcat
    26941 ctttacattg aactactcct gcgtgacttg tgtaaccaag ctttttagct gcattaacta
    27001 gagcattcgt tacctccaag tcagctacag caggaaattc aatcggagca tattctttac
    27061 ttgtaccttc catacggata gctccagttg caataacgat gtctccgcct ttaacatcaa
    27121 ggtcaatacc accacatgtt ccaacacgga taaaggtatc agcgccacag agtttcaatt
    27181 cttccatcgc aatagacgct gatggaccac caataccagt tgaagtaaca ctgacttttt
    27241 caccgtttaa tgtacctgta taagtaacat attcacggct gtctgctaca agaactgcat
    27301 tatcaaaatg ttctgcaatt tttgcacaac gttttggatc acctggcatg ataacataac
    27361 gacctacatc acctggacga atctgtaggt gatattgcaa tccgacttca cctgaataat
    27421 tctgcatgat aaagacctcc ttaatgtctt gtctattttt ttacacttgt ataataacat
    27481 acaagacaac ctttgtaaac gcttttatcg aaagaaaacg ttttattttt taacttgatt
    27541 tttctaattt tttcaattaa actaacttgt atacggaggt tacaatgcct aaaaatgaac
    27601 taaataataa actaaataaa ctaaagcatg tgcaagtcta caataagatt tttaacatga
    27661 tccaagacgg tacttattct cctggaatgc aattgccttc tgagcccgaa ttagcagggc
    27721 aacttaatgt tagtcgagct actttacgaa agtcattagc actattacaa gaagaccatt
    27781 tggttaaaaa tatcagagga aaaggtaatt tcattagaga aaattctagc aacttgtctg
    27841 aaaacggtta cgaaaataga caacacccta ttaaaacttg tctaacttcc aaaattacag
    27901 aagtggaatt agaatttcgt gtagaggttc ctgcagaagc tatcacagct tcacttaaac
    27961 aagaaacccc tgtagttgtt attgctgatc gttggtacca caccgatgat ggaccattag
    28021 cttacacact ttcttttatc cctatcgaac ttatttcaga tgctgagatt tctttacatg
    28081 atactaaaca gttgttaaac ttcattgaag aaggtatcta tcaagaaggc atatcaagcc
    28141 actcacaaag ccatttggga tatgcgacat ctggcaattt ttcagcaacc aaatataccc
    28201 tatccgatca tggacaattc atattaatac aagaaacaat ttttaaccaa gagaaaatac
    28261 taatgtgtaa taagcattac gttcccattg agcactttga attgtccatt acctctcatt
    28321 aaggagacaa gcctatgcaa aatagactat taaaagttgc ttttttcata acttttaccg
    28381 aattaattat gggcctaata gttggcgctt attttaagtt aagttatttt ctagcccctc
    28441 caaaggaacc gtcaatgggt gctgggtacg gaatgctctt tgctatgtta tttatactta
    28501 ttagttactt aattctatta gccataattc aattcttagc ttatcaatct caatctaaga
    28561 taatagcaat cattgttttg ctactttttt tcctcccact actggcaaat attggatggc
    28621 ctatccttat gatcttctta ccgcaagtgg tagtgactat catatctcta ttatcacaac
    28681 ataaaactcc cctttaacaa aggggagttt tatgttgtga taagttagtg attagaagcc
    28741 acccatcata gatggatcca ttgcaggagc tgtaggagct tctggttcag gtttatttgc
    28801 tactactgct tctgtagtca agataagact tgctacagat gccgcatttt gaagtgcaga
    28861 acgtgttact ttgacagggt caatgatacc tgtggtaacc atatctaccc attctccatt
    28921 agccgcatta aatcctgtac caatttcaga ctgttttaaa cgttcaataa taactgaacc
    28981 ttcatatcca gcattgtaag caatttgacg aacaggctct tcgagagcac gaagaacaat
    29041 attacgtcca gtctcctcat caccattaag tttcagtgcc gctacttttt caataacgtt
    29101 cacaagagcc gtacctccac ctgaaacaat accttcttca actgcagcac gcgttgcatt
    29161 taacgcatct tcgatgcgaa gtttcatctc ttttaattct gtttcagtcg ctgcaccaac
    29221 tttaattact gctacaccac cggctaactt ggcaagtcgt tcttgtaatt tttcacgatc
    29281 aaaatcagaa gttgtagcct ccatctgtga cttaatgata gctacgcgat tagcaattgc
    29341 tgatgagtca ccggcacctt cgacaataac agtagaatct ttatctactg ttactttagc
    29401 agactgtcct aaaacttgca tagtagcatc ttttaagtct aaaccaaggt cttcagtaac
    29461 gacagttcct cctgttagga tagcaatatc ttccagcatg gctttacgac gatcaccaaa
    29521 tccaggcgct ttaacagcta cgacattgaa agttccacga attttattaa gaacaagcgt
    29581 tgggagagct tctccatcaa catcatcagc gatgattagc aatggacgat ttgttttaag
    29641 aacctcttct aataatggta aaatttcttg gatatttgaa attttcttat ctgtaataag
    29701 gatatacgga ttctcaagtt cagagaccat tttctcgtta tcagttacca tatactgtga
    29761 caagtaccca cggtcaaact gcattccttc tacaacttca agctctgttt ccatacctcg
    29821 cgattcttca atagtgataa caccatcatt acccacgcgc tccatagctt cagaaatata
    29881 ttccccaact ttttcagaac gtgaagacac agctgcaact tgagcaatag cttctttgcc
    29941 tgaaactggt tgtgcaatct cttttagctc ttcaactgct gctgaaacag ctgtttcaat
    30001 accacgacga ataccaatcg gattcgcccc tgcagttaca tttttaagac cttcccgtac
    30061 aatagcttgg gtcaaaacag tagcagttgt agtgccatcc cctgcaatat cattagtttt
    30121 tgaagccact tctgacacaa gtttagctcc catattttca aagtgatctt ctagctcaat
    30181 ttctttagca attgtcacac catcatttgt aattaaaggc gaaccaaatg ctttttcaag
    30241 aacaacatta cggcctttag gacctaatgt tactttgact gtatcagcta aaatatcaac
    30301 accacgcacc attgctgagc gggcatctgc tgaaaattta atatcttttg ccataattta
    30361 atacccaggc ctaaaattta acaaagtcta ctttctttac aaagcaactt ttctctaata
    30421 ggcctatatc ctttcttgct taataaaacc tatttaacga cagcaacaat gtcactttca
    30481 cgaacaactg tcactttttc gttaccgtct ttaacttcca aaccagcacc attttcgact
    30541 aaaacagtat caccctctgc aacgctaggt gcaactaatt cccctgttaa ggtacgaata
    30601 ccgtcaccca cagcaagaac tttggctgtc tttgtcgcat catggcttgc tcctgcaaga
    30661 acaaaaccac cgacagattt ctcttcagtt tcaacaaacg aaatgatcac acgatcacct
    30721 aatggtttta acataataaa cgaacctcca tacgagatat attttgattt tttagcactc
    30781 ctatacagag agtgctaact acaaaaacta ttctatcact tggtcagaat tagtcaagag
    30841 aaaaggcacg tttcaaacaa gaaaaatttc cctagttatc ctgtccattc actagagaaa
    30901 tttatatttt aacctaagac aagggcatca tctatcaact gttgacctga gtttttatgg
    30961 aaaagttcca ttaattctgc aactgttaag tttctctttg cttctccttt aacatcaaca
    31021 acaattttcc cgtgatagag catcacgaga cgattaccat aagcaatggc atgttccata
    31081 ttgtgtgtaa tcattaaggc tgtcaatcgt tgttcttcta tcactttctg tgttaattcc
    31141 ataaccatat cgctagtttt gggatctaga gccgctgtat gttcatccag taataataat
    31201 ttgggacgaa caagagttgc catagctaac gtgagggctt gacgctgacc tccagataaa
    31261 aatgcagcat cagtcttcat tcgattttct aagcctagac ctaattgtga tagagacttt
    31321 ttaaatagtt gacggtcatc gtcagtcact gactgtctaa aaatgtgacg cttattgcct
    31381 cttttatgtg caattgccat attttcttca atcgtcaaat ttgttgcagt tcccattcta
    31441 ggatcctgaa agactcgact aatatcctta gaccgttttt ctacagagtc tttagtaatt
    31501 gattggttgt ctaatgtgat tgccccctga tcaatagaga ttagaccagc aatacagtta
    31561 aggagggttg attttcctgc accgttacct ccaattactg aaataaaatc accgtcttca
    31621 atcgttaaat ctaaaccacg taaaacatgg ttctcattga cagttccttt ttcaaaagtt
    31681 ttgtgcagat tgactaattc aagtaatgac atgttcttat ccttcctttg taagcgattt
    31741 agaggttttt actcctagtt tagctcgtaa ttctgggaca tacaaaatga gagccaacaa
    31801 aattgctgaa accaacttta tcatctgtgc atcaatatcg gttgttaata tgaaaacaat
    31861 aatcatacgg tataaaacag aacctaatac aattgaccat aaccttttgc caagaggtaa
    31921 gtattttatc attacttctg ccaagataat tgaagctaat ccaataacga ttgtgcccac
    31981 tcccatattc aaatctgcat agccattatt ttgcgctaaa agtgcaccag ataaagcaat
    32041 caggccattc ccaatcatat atcctagcat tttcatccga tctactttaa tactattggc
    32101 ttgtcccata gcctcattat cacctgtcgc tcttagagct aatcctagtt gggtatttaa
    32161 aaagagatac aaaattaaga taaccaagcc tacacaaaca attccaatta ataaaactgc
    32221 tgaaagttta tttaaaccta atcttgttac cattgtaacc aaagtatttt tgagagcaaa
    32281 agagacgttt gatcttccta aaacaagtaa attaatagag taaagtcctg tcaacgtaat
    32341 aatacctgtg aggagggctg gtattttcat tttagtatga agaaaacccg atactagtcc
    32401 tgccaacata ccacctaaca tgcccgctat ggttgccaca attggattaa tatcattaac
    32461 gatacataag gcgcaaactg cagcccccat agggaaagcc ccttctgcag agagatcggc
    32521 tatgtctaag atacgaaaag tgataaacac gccaattgcc ataattgacc aaagtaatcc
    32581 ttgcgaaatt ccggataaga taatatctaa catacaatac ctttctgatt atttattatt
    32641 tttaatactt gaaacatcaa ttccaagctc ttttgccata tcttcattaa ctactacttt
    32701 tagattttga ggatattctg aaggaacttt agcgacgtct ttacctttga ggattttaac
    32761 agcttgtctt gctgtttgac gacctaaagc ctcataatca gccccataag aaaagagcaa
    32821 accacttggt accatatctg ctgaacctcc cacaacaggc acttttaatt ctttcgatag
    32881 attccctaac agcgttactg agctggctat aatattatct gttggtacaa aaagaacatc
    32941 tgttttagac attaaactct tagcagtatc ttgtacatca ttagtggaag aaataccttt
    33001 aacacttgtt ttaatccccg cctcttggaa gatctttttt gcttgtttta cctgaacttc
    33061 tgagttacgc tcgctagtgg tatacataat accaacacgt ttagccttag gcattacttt
    33121 tctcaaaagg gatacttgct ttttaattgg agacatgtca cttgtaccag tcgctaaacc
    33181 ttcaggtttt ttcattgatt ttactaattc ggctgatacc ggatctgtca ctgctgtaaa
    33241 caaaataggt gtttctgttg acacagttgt caacgattga gccgccggag tcgcaattcc
    33301 caatactaat ttattatctt taatgagttt ttcagaaatt gattggatat ttgactgatc
    33361 cccttgagca ttcttatact caattttgat atttttgcca tctttatagc cttctttagc
    33421 taattctttt ataaatcctt ttctagtggc agttagagag ggatgttcta catattgtaa
    33481 aataccaata tgttttacat cagattgacc attttgagaa gattgtgaac aagctgtcat
    33541 cagaacagtt gctaaaacgc tgctaattac catctttgtt atttttttca ttactgtctc
    33601 cttgtaatac catcaaattt tccataaaac aaaaaaccaa ctagacccta catctagttg
    33661 gttaattatt tctctcaatt cctctgtgca cgagaggacg aaaaaatatt gccttagcca
    33721 actagattat tgaaatgatc taatcggctc tgctttcatc ataaaatgct ttagcttcat
    33781 agatacaaac tttgcgtgac gtttgtatcc ttgaggctac aaacgtctat ctaaagaaac
    33841 taaagtaata ataagtattt gtttgtttaa taatagacga tttgattaac atattattct
    33901 ctccttgttt atggttgcct ataagtttac gtcatttaaa aataattgtc aatagttaaa
    33961 tttgattttt ttgaaaattc ttgttagata gataatttaa ataatagtca ttcgttgata
    34021 taacacgctt ttaaagaaat ttagatttgc tatccttaat tttccatata ctctactaaa
    34081 gcataataaa taccatcgtt atcattatcc tgagttacat ttccaattct ctcttgtaaa
    34141 tctttaggag cattcgccat gacaaaacct ttacccacaa tttcaagcag atttaaatca
    34201 ttatagttat caccaaacgc aatagagtct gcaagagaaa tatctaaata gtctgctaaa
    34261 gttttagcag attttccttt ttcaatacct ggagccatta tttcaataaa gtaaggagca
    34321 gattgtgcga ttgataaatg tggatattga gctttcaaag tgttctccaa ttctcccatc
    34381 agagatggtt ctcccatcaa gagaagttta tgaatcctat cttgcttctc taactccatc
    34441 aaactcatct ctttagattg taaaccaaca atctcctcct ctttttggac ccactcattt
    34501 tctttatcac aggaatacca ttcatgataa cgatagacat tccatgcaat tttcgaataa
    34561 tgcttgctta cataagaaca aacttgtaag gcaacttgac catccattgt gtgggaagca
    34621 attgtttttt gttgcatatc ttgtattaaa gccccattat aggaaataat ataagaatct
    34681 attccaattt gttccataat cggagtaatt gcctcaggca tcctagctga tatgggaacg
    34741 aaagtagccc cttgagctac tatttgttta attccctgcc gtgtcttagg tgttacttta
    34801 aaattactgt taattaaagt accatcaata tcactaaata ttagccttac catattatcc
    34861 tccaaaatat aattgtacgc gcttacttta gtctaacact taaatttttg tttcgcaaat
    34921 aaacttcgtc tggtctaatg agactactaa tataagctat atttcaaaac aaattagttt
    34981 atttctctca atgaacggtg taatatgtta ctatatagta ataacaggag gattaactat
    35041 gccgtatcca tacaaagcca atcattccat tgaatcaatc accttaaaag taaatgacct
    35101 cgaaaacctc gtaaacttct atagcgatat cattgggtta actgttattg acaaatccag
    35161 cactagagcc ttactagggg taaatcaaaa aatccctctt attatccttg aaaaaactga
    35221 acttgaaaag catagtacct atggccttta tcacactgct attttagtac cagatgaata
    35281 ccatctcagt ctagctctaa atcatttatt atctcagcat atccctctag agggtggagc
    35341 tgaccatggc tatagcaatg ctatctatct aagtgatcct gaaggtaatg gaatcgaaat
    35401 ttataatgat aaggatatct ctatgtggga tatcagagag agtggacaaa ttattggtat
    35461 tactgaaaga ctagatattg acaatttact tgacagcctt gtcaacgttc ctaataatta
    35521 taagctttct gaaaaaactc gtattggtca tattcatttg agtgttaaag atgctaagat
    35581 tagctcaaaa ctctatcaaa atgtttttgg ctttgatgag aaatttgcaa tcccgacagc
    35641 tagttggatt gcatccggca actaccatca tcatctagcc tttaataatt gggctggtcc
    35701 aaatctttct aaaaaccaag aagatagacc cggaattttc ttattgacaa ttgcttacaa
    35761 tgatgacaac ctttttagag atagcttaaa aaaagcgcaa ctttaccaac tgactttcct
    35821 tgaaaaacaa gatcattatt atattattga agattttgat ggcatccgaa ttaaagttgt
    35881 tctaaaacca gagaattaac cattacttga cttttgcctc ttcattttac ataatgtcaa
    35941 tatgataaaa attttgatcc caactgcaaa agaaatgaaa gtgtgtcaaa acattgcaag
    36001 gcctaaactg agtgcacaaa cgcaaattat aattgattat ttttctactt taactgtttc
    36061 agacttagag gatatctaca gaattaatac aagtgcagct cgttgtgagg ctcaaagatg
    36121 gcaagatttt aaatgtaaac aacttactct gaatcctgct attaagttat tcaacggtct
    36181 tatgtaccgc aatatcaaaa ggcacaactt atccataagt gaagcacaat ttatggaaaa
    36241 ttctgttttt attactagtg ctctttatgg catcattcct gctatgacac taatatcccc
    36301 tcatcgttta gattttaaca ctaaaatcaa aatcaacaat aactctctta aagttttttg
    36361 gcgtgaaaat tacgatactt ttatgcagtc agatgacatt atggtttctt tgctttctaa
    36421 tgaatttgaa accgtctttt ctcctaaaga gcgtcaaaaa ctaatacatc ttaactttat
    36481 tgaagatcga gatggccaat taaaaaccca ctctaccata tcaaaaaaag caagaggaaa
    36541 gtgtttaact gctatgatgg aaaataattg ccaaactcta gaacacttaa agcaattacg
    36601 ctttgatggc ttctgttatg ataatgaact ctcagactct aagcaattaa cttttgttaa
    36661 aaaacagact taagtaacta tttatccaca atttattcac acaaaaagca agagataatc
    36721 gtatctcttg ctttttgaca aacttgtaag gtctaaaaac ctctctatat caaggtttca
    36781 aataatatta acaaaaaata attgttatca acaacttgtg gataactatg gggatagaca
    36841 gaggataatc tgtttatata aagttgatta cttaacatta atgtcatttt atataccttt
    36901 agatttcaaa aatacttgtt aaattaacaa taataaacgt caacatcttg tcacagactg
    36961 tcaaatactt ataaacaagc tgtggataaa cataactttc aatttctatt taaaagttaa
    37021 tttttgacac taaagtaaat catctcgttt gacttgttca taactattgt caccatcgtt
    37081 taatccttcc caaatcacga cttcaccttc ttttaaagat ttttgtacat caatgattct
    37141 ttgatttgaa gatcctctaa attgtagcat taaatttcgc ttactttgat caaacctacc
    37201 atccactaaa atatcaatca aagataacat ctccaattta tcttgagtct ccagcatcat
    37261 ttcttcccaa gtataccccg tccatgacca aatatctttt tctggcaatt cacgtcggat
    37321 acgctgaaga agtggcagca aaatacctgt atttaagaac ggctctccac ctaataacgt
    37381 taaaccttgg acgtaaggtt gtgccaaatc tttcataatt tgatcttcaa gctcttttgt
    37441 ataagggatt ccggctcgaa aggaccatgt agcagtatta taacaccctt tacagtggaa
    37501 catacatcca gctacgtaga gtgaatttct aacaccttca ccatctacaa aattaaaagc
    37561 tttataatca atgatatggc catgacttaa ttcttcgctt ttccactccc caggctttgg
    37621 agtattccaa cttgcttctt ctgacatttc taacctctct attgatatga aaagaaccag
    37681 aactaactgg tctttttatt attatcttaa agtactattt taggttatcc aataacgctc
    37741 tgtctgatga cgacaatctt ctaaaacacc accgttagct aatattactg acttgctggc
    37801 atcattgtct acatggcatg tcacaagtat ttcattgata ttttttagat gtgcttgctc
    37861 aattcctagc ttcaactgtt ctttagcata acctttgcca cgctggcgag gtcgtatgga
    37921 ataaccaata tgtccacctt tttctaaaag agtatcattt agtcttaaac gtaaatttaa
    37981 gaaacctata gcattattat cattgtcaaa agacacaaac tgaatatatg gaacaaaatt
    38041 atcaggaaca cctatcccta actccatatt taggttatga tctagccagt cttcaaaatg
    38101 catagttggt tcataaaaac caccatccat tgaactactt tctttttgaa actctttata
    38161 catggctaaa atttcttctt tatcttctaa cactgggcgc cgtaatttca tattgtttcc
    38221 tattccatta tcttcaagtt aagcattcca aaaggagcat tcgctttatt aatctggtca
    38281 caaataaaat gactcaataa actagacttt ggtgatatag gattgacacc tgtagtgata
    38341 gcttgaataa aagcatcaat cattggttca aaaccacgtt tctctagagt gcttgcccaa
    38401 gagccaaaac cgatagcacg acgatcaaag ccatcaataa cagaaagatt tgacaaatca
    38461 tctaatgaat aggttacttc tggcgactct atttcaatta tttctcgacg agaccccgac
    38521 tgcaggttca tgcttgcttc tattatctca ctgtctgttt ctagcgttac tgatacttgt
    38581 aatatcttat tcccatcacg tttagtcaca aagtatcctc ttttaacaac attatttgtc
    38641 aaaaatagcg ccgtatctaa aggatggata aacatatcaa aaagcttata ttgaaaatcc
    38701 gctggtgcat tgacagcatt tttaaaggtg cgaatatggt ttttatcttc tacttttttc
    38761 atttccataa tacgtggtgc aaagcgacga ttaaaacctg ccattaaaaa tgttttatga
    38821 tccttcgcca aatcatacaa tgctttagtt gaagtataat cttctgtcaa aggcttatcc
    38881 ataaaaactg gaataccctt ttttaaaaaa agctttgcta tatcaaagtg agcactagtc
    38941 gcgacatgaa tgaagactcc atccaaagga tgctcggcta gactatctaa actgtcataa
    39001 gctgtagaag aaccaaaaag catattaact tcttctaaaa tctgtttttg gcgtgtaaaa
    39061 agatgccagt gcacacctgt cacttgacgc atataaggta gataagcttt ctgtgaaata
    39121 cctcctaaac cgacaattcc aagttgcaac atagcatcct ccttctattt tttctttgcc
    39181 ttttccaaaa attgtttcct taaatctttt agacgtgatt tttgattagc attctctcca
    39241 gtagagtgct tctcatggaa cttctgtact tgtgcttgcc ctttgtaatc taattggtat
    39301 ttccccatat cttaataact ccttctattt tatcttctta ttttaacata taatagacca
    39361 gaggttttaa tagaggtttt aattaattac cttgattttt aatacttgat ccgttcatat
    39421 gttttacacg agcagagatt tccttatgac gaccattaac catcggacga gcctgtggat
    39481 tacctaagta accacatgta cgtttgacaa catctactgt cttagggtcg ctgttaccac
    39541 aatttggaca agtaaagcct cgatctgtcg gtgtaaaatc cccttcaaat tgacattggt
    39601 aacacttatc aattggtgta ttagtaccta gataacctac ccgatcataa gcatagtccc
    39661 aaactgcttc taaagctttt ggattctgtt gcaaaacagg gtactcacag tagtgaataa
    39721 atcctcctga tgcacccgtt tcaggataga ttttctcaaa atctagtttt tcaaaaggtg
    39781 ttggattctt acgcacatca tagtggaatg aattagtgta gtattcctta tcagtaatat
    39841 cagttactat accaaatttt tcagtatcta aacgacagaa ccggtcagtc aagctctccg
    39901 atggtgtcga ataaactgaa aaatgataat catactcatc agaccaatct gcacataact
    39961 gcttcatctt acgaacgatg tctacagtaa atgctttagc ttgatgatta tcttcccagt
    40021 caccaccata aaaaacactc gccacctcat ataaaccaat ataaccaaga gacaccgttg
    40081 cacggcggtg attaaagagt cgatcaacat tgtcatattt tcctaaacgt tctccaaatg
    40141 ctccatactg gtaaagaatg ggcgcatttg ctgggatagc ctctttgaca cgttccacac
    40201 gataaaccaa ggcatctcta gcaatactca tacgttcatt aaatatctcc cagaatttat
    40261 ccatatcacc gttagactcc atggcaatgc gcggtagatt aactgttaca actccaagat
    40321 tcatacgacc agatgttaca tcctgaccat ttgcatctcg ccacccttgt aggaaagaac
    40381 gacagcccat aggggcttta aaagagcccg ttaagtcaat aattttatca taagaaagga
    40441 catctggata catccgtttt gtcgcacact ctagtgctaa ttgtttaata tcataattgg
    40501 ggctattttc ttctaaatta aggccttttt taagcgtaaa aattaatttt ggaaaaatag
    40561 cagtacgatg ctcgcttcct aaaccttgaa tccgaatttt caaaatcgct ttttgaattt
    40621 ctcgttcaaa ccaattagtt cctaatccaa aaccaagcga agtaaatggc gtttgcccat
    40681 ttgaagtaaa taaagtatta atttcatact ccaaagattg catggcatca tatatatctt
    40741 tttgtgtttt agcgcgcgca tagtcttctt gtttatcttc aataacccat tcttttgcat
    40801 cttttaaatg tttttgataa tttaactgtg cataaggagc taaaaactca tcaatgcgat
    40861 ctgctgtaca gccaccatat tgacttgaag caacgttcgc aataatctgt gaaatctgag
    40921 cagtcgctgt ctgaatagat tttggactct caacttctgc attaccaatc ttaaaaccat
    40981 ttgcaagcat tcccttaaaa tcgattaaac agcaatttgt catgggagtg tatgggctgt
    41041 aatctaaatc atgataatgg atatcaccct tttgatgtgc atttgctaca tgagaaggta
    41101 acattttcag accaattgat ttccctacaa tacctgctgt taaatcacgt tgggtattaa
    41161 acacatcaga gtctttatta gcattctcat taacaacagt ttgatcctta ttaattaatt
    41221 ttccaataga aaaattgata tctgtagcct gtgaacgctc aaaatcgcgt tgggtacgat
    41281 aattgatata ttcttgtgca atagcgtact cattagcttc caataattta tgttcaacga
    41341 tattttgtat ttcatatatc ttgatgttat ctttaaaacg attgtagatc tcagcgatga
    41401 catgatctgc aatcatctca agtttagcct caactaaagg agacattttg ataactttat
    41461 tactagcttt taatagagcc gtgtagattt tatacttatc aaagctgacc aaacgcccgt
    41521 cacgtttaat cactttgata tcagattctg tcataaatcg ttctctttcc aataccatca
    41581 tgtgaaatat cctccagtga caattatagc atgcaaagaa aaaaaatcaa tatattgtgt
    41641 caagtttttt gctcagacac aatatattga tttttaatct atttgaaacg atatagaaca
    41701 aaatgagtga tatttgatag aggaacttct tggtagtgct tcttaatatc agatttcagt
    41761 tcatttggta acttctcatt tttatttaag ataaagtatt tagccccatg ctgaagcagc
    41821 tcgtccttca aaattttctc attatttttt tgcgctgtat taatatgtgg caaaacaaat
    41881 tgagaagcag attttaaccg agtctggcta taaatgctag caactttgtc ccaaacataa
    41941 atcttaccat ctctatcaat ttttgtagtc aaataatgtg aaatgtcacg cctttcttta
    42001 ctaagagtaa cattttgata agtattataa actagtaacc ctatcgataa agaaactagt
    42061 aagaggggca aataatatgt tttctttaca aaaaggtcta cgataagaga tttaccagag
    42121 aaatactgac gtctatgtga acgcctgtca atcacttttt ggtaaagaat agtaatttta
    42181 ttactcgtta gcaaaagacc aaatggtaaa gcaggtaaaa agtggtaaag gttaaaatct
    42241 cttgaaaaaa tggccattat agcgtaaata ataaaaatac aggcaataaa gttcaagccg
    42301 atttgataag atgctttttt acgattttga ataaaatccc atagtccaaa gagacaacct
    42361 gaccctagaa ggacaacaat ttgaattgct aaactaagaa ggaatgagtg gtttgtccta
    42421 gcgaaatacg taaaggggta aaaaatagct ttgtcaatat aagaagagat aatctgtaga
    42481 ttaaacataa agtaaccaac agtataagca attaaaatca taccaaaaac aacgcataaa
    42541 aactgataaa atcctcgtag tgactgcttt tgcttaatat tggttgctgt taagagaaca
    42601 aaagagcaaa gccaaaaaat cagtgtttgg ggatctataa atagtgagaa tgctgctaaa
    42661 atgccataaa atataaatgc ttcatcaacg atattatcga cacaaaattt aattaaaaac
    42721 catagcgaaa tgagcataaa aggtaatgcc cactgtattg ggtacatacc accgaatcct
    42781 aagacaatat tcattatata gaaaatcaac gtcatgccta tcgagacaat ttcactttgt
    42841 gccacataat aaactaattt ataaaaataa attccagata catagtaagc aataaactgt
    42901 acaataagta accagatact agaccctaaa aggtaactta gagcaatcgt tacatagtat
    42961 aaaaatcctc ctgtagcaaa aacatcacta taaggaatct gtcctttggt catcatcaaa
    43021 cctgtataga gattctgagt ctgcaaacca tttgcaaaat aagtcaataa cggatttgca
    43081 attgctaaga gagttgacaa aacactatat aataaaattg aaaatcgact agatttcttc
    43141 attctgatag ccgttcgttt actcattgat gggctatttt tatagctcac tccaattggt
    43201 ttttctaggt tttctaccac tatttttctc ctcatattta catactacca gtatatcaga
    43261 aaaccttatt tagtcaattt agccaacact tgaccgtaac gaataatatc cctaaagtag
    43321 tggtattttc tagacatata ttgtttgaaa aattcttgtt cttctaatgt tagctgagta
    43381 tctgacatta acatcacctc cttttactac atcatcacta ttattattcg aaaaaaaaaa
    43441 cgactgaaca attgctcaat cgcttcaata ttattcttca tctagaaagc tgttgaaaac
    43501 ttcctcaatc atgtcccatt ctgcatctga atcttctggt ataggctgaa ggtctccttc
    43561 agttccatcg gcattttctg taaatgagta agcttgaatc tcaatttcac cttgttcgtc
    43621 ttcttccgca cctgcaggaa tgaggagaac atagtttttg ccaaactctt cacgcccatc
    43681 aatagttaat aggatttcaa aaagtgtttc gttcccattc tcatctacaa gagtgattac
    43741 ttcatgttga tgttcatggt tatgatcgtg gttatgatta tgtgccattg tttctccttt
    43801 ttatgccgaa tgcttaaaac atgcggtcta aataattttg taagattagt tgagctgcta
    43861 atttatcgat aactttctta cgcttaccac gactaatatc agcttgctca actagcatac
    43921 gctctgcttg aacagtcgtt agacgttcat cttgatattc aactggaaga ttgaataatt
    43981 cagtgatttt atcgccatat gcctgactag cctctacacg aggaccactt gtattattca
    44041 tatttttagg tagaccaact acaaatttat caactttgta ctcttttact aactctgcta
    44101 agcggtcaaa accaaaattt ccagattctt catcgatctt aatgatttca agaccttgtg
    44161 cagtgaaacc gagtggatca ctaattgcaa cgccaacagt tttcgagccg acgtcaagtc
    44221 ccattattct cattacaaat ctattccatt tccttgtaaa taataacgaa cgagctcttc
    44281 tacaatctca tcacgttcat atttacgaat ttgattgcga gcatcattgt aacgaggtac
    44341 ataagcaggg tccccactca atacgtaacc aacaatttga ttgatgggat tgtaaccttt
    44401 ttcttctaat gaacgataaa cagctgttaa tgtttcacta atttctactt tgttactatc
    44461 atctaatcta aaacgaacag tttcatctgt aaatcccata cctacacctt ctttccttta
    44521 attctttcac ttattataac ttaaataaag tatttgtaca agaaattgat aaacactata
    44581 ttagaaaatc aaaaagggag cacaagaaac caagaagtga ttctttgatt tctttactcc
    44641 ctaccttata ggtgattttt acaggttatc gtgtgactct cacttatgct tatctaaagc
    44701 tcaaagtgct gcacgaagac gagcttctgc attttcaacg ttacgaatag agcgaggtag
    44761 aaaagcacga atatcatctt ccttataacc tacttgaaga cgtttgtcat caattagaat
    44821 tggactcttt aaaatacgtg gattttcttg aataagatca ataacttcat ttacactcaa
    44881 ctcctcaata ttacaattga gcgccttagc ataacgattt ttagaagaaa cgatactttc
    44941 aataccactt tcagtttttg ttaatatttc taaaatttca tcacgtgtta gtgattcttt
    45001 acctaggttt tgttctttat atggaagttg atgagcattt agccaagttt ttgctttttt
    45061 acaacttgtg caacttgaaa tagtataaat cttaatcatg tgccttctca ctttctatcc
    45121 catgatagtt atagtttaac atagtcacaa aaatcaaaca tgggacttaa gacctatttt
    45181 ttatcaattt actaataaaa ataaatatat tttagggatt tttaagctta tttttagtat
    45241 aaaaataagt tccaaccact gtcagaactt attttagtta catttattca agttcaagat
    45301 gtatagttgc tattttattc ctcaatttca atagcattgt ccaaatctaa aacaagatca
    45361 tcagcttcat ttttttcttc aacgagctca gactgaactg gcgaatcatc ttcagtcatt
    45421 ccaaagtgca cgcgcacttt atggtcaatt tcatcaaaaa ttgctggatt atctgctaaa
    45481 tattttttgg cattttcaga accttgtcca attttctcac cattatatga gtaccatgca
    45541 cctgcttttt ggataatatc caaatcacta gcaattttaa ttaactcacc cgtgcgagaa
    45601 ataccttcac catacataat ttcgacaaaa gcttcacgga atggtggagc aactttattt
    45661 ttaacaactt tgatttttgt ctctttacca acattatgat ctttatgctc accagtcccc
    45721 ttgatttgag tattaccacg aacatctaga cgaaccgatg aataaaattt caaagcacga
    45781 ccaccaggtg ttgtttcagg gttaccaaac ataacgccta ctttttccct tagctggttg
    45841 ataaaaattg cgattgtctt tgtcttgtta atggatgctg acaatttacg cattgcttga
    45901 ctcatcatac gagcttgcaa accaacgtgg ctgtcaccaa tatcaccatc aatctcagca
    45961 cgaggaacaa gggctgcaac tgaatcgaca acaactaaat caactgcgcc tgaatcaatc
    46021 aatttacctg caatttcaag accttgctca cctgagtcag gttgagacaa gagtaactca
    46081 tcaatattaa ctccaagagc cgcagcatag gctgggtcaa gagcgtgctc agcatcaata
    46141 aaggcagcaa tcccaccttc cttttgggct tgagctacgg catgaagggc aaccgttgtt
    46201 ttaccagatg actctggtcc ataaatttcg acgatacgtc ctttaggata tccacctgct
    46261 cctaaagcga tatctaaagc aagagaacct gaactcatta cttgaacctt ttgttcagct
    46321 cgctcaccta agcgcatcac tgcgccctta ccaaaatctt tttcaatatt tttcaaagca
    46381 tcatctaagg cttttcgacg ttcatcaccg aatttcttag taatttcttc tgcttttttc
    46441 gtttttttag ccaatgtctt ctccttatat ttctatgaat ctaggttgat tcaaatttag
    46501 aaatcgttct actaattata acaaatatta tatctttaat aaagccctac gcaccaggtc
    46561 aaaagcatgt aaagtactga tatgacggac atctgagcgg ctcttccctc caatcgatac
    46621 tttaatagaa gaaacgcctt caggggttgc aatcccaata aaaactgtac ctgcagggta
    46681 tccttccaac tcatcaggac cagcaactcc cgttaaaccg ataccaaaat ctgcttttaa
    46741 aagttggcga gcttgctgag ccattgcctc agctgtaaaa tgactgataa ctccatattc
    46801 ttgcaatttt ttcttaggga tgccgagaag ttgtgatttt gcttccatac tataggtcgt
    46861 aaaaccacca ttaaaaactt gcgaagcacc agaaaattct gctaactgag attgaaataa
    46921 ccctgctgtt aagctctctg cggcagtaat cgttttattt tgtactttca ataattctaa
    46981 aacagtacgt gccatactat tattatcacc ataaccatat aataaatctt ttaatttacg
    47041 gttatctaat gtcttaagag caagtatttc tttttctaag ctatctaatt taaagtcggc
    47101 ttcatcctga ttttcagctt tcgttgataa ccttaaagtt acctcaccaa cctttgcata
    47161 tggtgctata gttggatctg tctgattctt aattatgtct tctaagattg tgactaactg
    47221 actctcaccg ataccaaaga atctcaatac acgtgaatat aattttgtac cattatttga
    47281 caaaataggt accacctgct ccataaccat tggatttaat tcactaggcg gacctggtaa
    47341 aaccatatat tggactccct tagatgtaac gataccacca acagctagac cagtaaggtt
    47401 ttgtagaggt attgagcctt caaccaattg agcttgtcgt tcattatttg gcgttctagc
    47461 aaactgagga cgacttgcaa aaaaacgatc caatttttga ctagctaata aatccactgt
    47521 taatgattta cctaaaaatt tagccaaagt ttgtttcgtt aaatcatcct ctgtaggtcc
    47581 aagtccacca cataggataa ccaattgact gcgctgacta gctatatcta aaagcgataa
    47641 taaacgttcc tcattgtctc ctacagctgt ctggaaataa atatcaattc ccaattctgc
    47701 cattttctcc gacaaaaatt gtgcgtttgt attaacgatc tgtcctgtta aaatttctgt
    47761 tcctacagcg ataatttcag ccctcacaac ttcaccctct tatctattcg aaataatgac
    47821 tacattttag caaaaaaagt agcaaaagtg ctacttaaaa catatcacat tgatattact
    47881 tgaaatcaca tatattttca tggtcattaa ccatacccgc ggcctgaata aaagaataga
    47941 ggcaagttgg accaacaaat ttaaaaccgc gtttttttaa atccttagat aagcgttcgg
    48001 ataaggtagt acttgcaggc acatcgttgt agttgttaac tgagttaacc tgtactttat
    48061 tatctacaaa agaccaaatg tagcgatcaa aacttccgaa ttctttctga attaatataa
    48121 ttgacctagc attttgtcgt gttgagaaaa gttttagacg atgacgaata accctaggat
    48181 tttgtaagat tatctctaaa tccgcatctg acattgcagc taccttttca atattatagt
    48241 gatgaaaaac ttgtctgaac tcctgacgtt tatttaatac tgtttcccaa gataaacccg
    48301 attgataagt ttctaaacat agtaactcaa agagtacatg atcgtcatga acagctctcc
    48361 cccactcttt atcatggtaa gctacatata atggattatc taaattcacc caagaacatc
    48421 gtttcatgtg aaacctcatt acttctaaat tatttcatca acatcttcaa actagatttg
    48481 atgtattgct ccacagtctc gttcgtacct tcaaaaaagg cttttacttt tttcaattca
    48541 gtcgccttat accccaacgc taacaaagct tccatagctt cttcaagatt gctattctgc
    48601 tcagagctaa ccttacgact agtcgcactc tcaccagaag cttctacaaa cttccctgac
    48661 aaatcaagga tcatctgttg tgctgttttt tttcctatct ttggaaattt agtcaaatac
    48721 ttaatatcac tattatcaat ggcacttacg agaccttcat tgtcatcgac tgcaataatt
    48781 gctaaagcgg tcgttggacc aattcctgat actgaaatta aattaagaaa aatttctttt
    48841 tcattttccg tatgaaaacc aaataaaaga tgtgcatcat cgcgaatgac ttgatgtaag
    48901 tagatggtaa cttcttgatt aacgtaacct gaaaaagagt agggatttgc cacatatatc
    48961 acgtatccca atccagctgt ttccacaaca ataaatttag ctgttatttt actcaatttt
    49021 cctttaatat aatcgtacat tctattttcc tatctcaata tttttcattt tggtagcata
    49081 taagattaaa accagacctg ctaacataaa aatggtcaat gttatttgaa cacctatttt
    49141 tcctgtaaaa tagctgagcg aaaccatggc acctgctaat acacttgcaa caggttgtgc
    49201 gatagctaaa aatccagaat atgatccaat ttttccctcc accatcatct ccgcccttag
    49261 aacctgacta gctggaacat aaatcatttc gccaaaggta aaaaacgtca tggcaatcaa
    49321 aatagctcca aaagtgttta gagagatatt aaaaagcatt ccaaaaccac aaatcaagct
    49381 ccctaatatt agttgacgtt tcaaaggcca attctctatg aatttattta tagttgtcat
    49441 tagtaagaca attaatagag tgtttgtaaa gactgctaaa ctaagcattt tagcacctgt
    49501 gattgtatgt cctaaaatgg acactacttc aaaattttgc ttaagattga ccgagaaata
    49561 attatcaacc tggagccaga caacactgga accaattgct cctaaagtat aaacaacaaa
    49621 cgctttatcc actaaaacct gactataatt tttaaaagtt cctaaaactc ccttatcatg
    49681 cttgaaggtt ccctcctgtg gttttgtctc atcaaacttg aagtaaacca cgaaacaaca
    49741 aatcgctgaa ataataagaa gaactattaa taattcaaaa aaatggtgat catagaaaat
    49801 cccagcaatc ccagaaccta gcattacagc gatgttaacc aaccaataac ctatagtgta
    49861 aacaaagcga cgattctgtt cgttcgtcaa atcaatggtc atagcttcat aggcagggaa
    49921 ataaaattgg tgagctattt caatgataag gatacctaca aaggtcaagt gtggcgtaat
    49981 atgatttgga acatttgcag ctatagttat agcccaacca atggttgcca gcagtgaacc
    50041 tacaatcaca acttttttcc tccctatagc atctgacaaa tggcctccat ataaggtcgc
    50101 tacaaaccct gatagttgag ttatgatgat caaaattcct gtaactagat ttccaaaata
    50161 ttgaacataa tacattgcca taaaaggaaa aatcgctgaa ccaactgtaa tggaaataaa
    50221 acgcaataat tctcttaact gtagctgttt tggcaaagca aaaaactctt tcactttttc
    50281 tcccttcttc cctaacaagt aattatcaat attttcctaa atccctgagg cttgtatgat
    50341 tttcttggat tcgtttaaac attttctcca tatcagcttt tgtaaaatta actaaaacag
    50401 gtcttccatg agggcaatta taaggatttt tacattgcgc taactgatct aataaatgac
    50461 gtgctgagta atcgtctagc gtatggtttg ctttaataga tcgcttgcaa gacatcataa
    50521 tagccaactc tgcgcgatat tttttcactg agacttcatt agtgagcaac aacatatcac
    50581 acatttcata aacgccagac tctacctctt cttctttcat ccaaattgga tgttccctta
    50641 aaatgaaagt attattacca tacggttcca agaaaatacc aacatcttgt aataatgaca
    50701 ttttttcttg taattgaaga aaatctgaac ttgaaaattc aaatagaaat ggtactaaca
    50761 attgttgaag actattatca acctcaccta tcttttcacg gtaatactcg tattttacac
    50821 gctcttgagc tgcatgttga tcaataatat acaacccacc attcccttga gcaaataagt
    50881 aggtcccatg catttgacca aaaaattcga gctctggaaa agtcgaactt tcttccgaat
    50941 ctaatttttc aacaagtttg ttaagtgact gtttactact aaggtagtcc tgagaatgaa
    51001 tgaagttctc actatcagct tgatctctac tagcatactt tatattagga gactgcgttg
    51061 ctccttgatt aacagagttt tttacatcat tgtcaactga accgtcactt ttgtcaaggt
    51121 ttgtaaagtt agcaataggt tcagatacag tatctgcatc tataaaaaag tcatttttag
    51181 tcctatcgta atacaaacca ggctgtttta gtgagaagct tgtttgtata ggcttatcta
    51241 cacttcgggt actagtttta gctaaattct ctaaagcatc cggaatcaaa tcatattgtt
    51301 taagactctc agaaattgct gagcttatta aactcattaa ttctcgttcc ttagaaattc
    51361 tcacctcttg cttagtcgga tgaacgttga catctgctag ataaggatct atctgaatat
    51421 caatgacagc aattggaaat cttccaacca tcaactttga accataacca tctagaattg
    51481 agcggtttaa aagaaaattt ttaatgtaac gaccgttaat taatagggta atatagtttc
    51541 ggttagctcg tgtcaattca ggcaaactta cataacccga aatttcaaaa tctaaatctg
    51601 catttgaaat ctcaatcatt ttttttgcag tatttaaacc ataaatacct gcaatcgcct
    51661 gtctcaaatc gccagtccca ctagttttag tcatttcttt accatcattt atcagtgtaa
    51721 aagcaacttc cggatgcgct agactgagtc tattaacaat atcaataata tgagctagtt
    51781 cagactgtaa actcttcatg tatttcaatc tggcaggcgt attaaagaat aaattttcaa
    51841 ctaaaatttt tgttccacga ggacttgaaa caacttcctg tttttcaatg tttccccctt
    51901 tagcaacaag taacgtccct tgtttccctt gctctgtagc tgtttttatc gtcattaaac
    51961 taatcgaagc gattgatggt agggcttcac cacgaaaacc taaggtccga atacgaaaaa
    52021 ggtcactctg gcttttgatt ttggatgtag cgtgacgtcg taaactgaga acagcatcct
    52081 cactagtcat cccttcaccg ttatccgtta tttgaatctt tttaagacca gattcttcaa
    52141 cctctatggt aatttggcta cttcctgcat ctatggcatt ttcaactaat tctttaacga
    52201 cgctactagg tctttcaaca acctcaccag cagcaatctg gttagctaat atatccggta
    52261 attcaataat tttagacaag ttcatccctc ctttttttaa gattttttgt aacttttttt
    52321 gtgatatctt agccattata ccatattcaa aatattaatt tgacaataac acaattttta
    52381 gttgacatca ctcctattac ctgttagact tagggagcgt aataagtttt gaagttgtat
    52441 ttttgtttaa catttttacc ttttttattt tttacagtaa catttttaga aaggtaataa
    52501 acaaatgaca caaggtacag tgaaatggtt taactcagaa aaaggttttg gctttatttc
    52561 tagcgaaact ggaacagacg tcttcgctca tttttcagag attaaagttg atggcttcaa
    52621 aactcttgaa gaaggacaaa aagtaacatt tgatattcaa gatggtcaac gtggaccaca
    52681 agcaacaaat atcaatcttg ttaaataatt aaaaaagagc ctaaatgaat tatttagtct
    52741 cttttttgtt gcccaaaggc tattttataa taatttcttt aagtcaaata tagcattcat
    52801 agcttgcatt ggagttagat tcattatatc tatcgcttct aactcctcaa taaccctacc
    52861 tgcatttgaa ggttcttcaa aaagtgataa ttgtccttgt tgcttctcag aatctagttc
    52921 ctcatttaag tcagcagtaa cagcttcttg gggggctgat acgatcaact gtactgcatc
    52981 agcttccaac tgtgataaaa tatcagttgc cctatccaat aagtcaattg gtaaaccagc
    53041 tatttttgcg acgtgtatcc cataagactt atccgcaggt ccagattcaa ttttatgtaa
    53101 gaaggtaact tctccatctc tctctaaagt agccacgtgt acattgacaa gccttgtcaa
    53161 ttgttcagat aaatctgtca actcatggta atgagttgca aacattgttt ttgccctaac
    53221 acggtcatga atatattcta taatcgattg agctaatgcc ataccatcat aagtggctgt
    53281 ccctcgacct aattcatcaa aaagaatcaa agatttatca ctggctcgtt ttacagcctg
    53341 attcgcttcc atcatttcta ccataaaggt tgattgccca gaaattaagt catcagcagc
    53401 accaatccta gtaaatattg catcaaatac aggcaaatca acttcgtctg ccgatacaaa
    53461 acctcccatt tgtgccataa taactgtcaa agctaactgg cgcatatagg tcgacttacc
    53521 actcatattt ggtcctgtaa tcaactggat atctgtctga ctatcaaaat agatactatt
    53581 gggaatatat tcttgcactc ccatcacttt ttcaacggtt gcatgacgcc cattcttaat
    53641 cttaatctga tgttcatcat taaatttggg acgaacatag tgatgatttt ctgcaactac
    53701 tgccaaactc tgtagaacat caacggttgc aatagtcttt gctagctctt gaagacgttt
    53761 aatataagat tctacttgag cacgaacacg cataaaaata tcatattcta aatttgaaga
    53821 ttgctcgcga gcttcgagca cttcaccttc aattttggct agttctgctg ttccatagcg
    53881 ttcagaattt tttaacgtcg ctttacggaa aaaatgctcc ggtactagtg ataaattgga
    53941 attggtaaca tggaagtaat aaccgtcttt tttattataa tcaattttaa gagtaccgat
    54001 accacttgct gctctttcct tagcttcaat atcagcaatc caacctgtac cttcacgcat
    54061 cactgttcga taattatcca attgcttatc aaatccagac ttgataatgt ttccctcagt
    54121 gatagtagcc tgtgcttctg gggctatcgc cgtattaatt aaactttcta attcaggcat
    54181 agtgtcaatt ttgttgacaa caatgtcaag ctcaggttga ttgaaggact gtaaaatcat
    54241 tttaatccga ggaatttgtg ataaggtctg tccgagttgc aatagatctt tagggttggc
    54301 ttttccgaaa gatactcgac ttgctagacg ttcaatatca tatacaccct ttaaactttc
    54361 tgtgagatcg tttctctcaa aaaaataatc aagaaacact tgaataatat cttgacgttc
    54421 cttgattcga ttcatactta ccaaaggcct gtcaatccaa gttctcagca ttcgagttcc
    54481 catcgctgtt ttagtctcat ctaacaacca gtaaagactt ccgtgcttct tgcttgttct
    54541 agcattctcc agtaaatcta gactattttt cgttgcatat gacatttgta aatagtcctt
    54601 tatttcataa tgaactactt tctgtaaatg actaagttca cgcttttgtg ttctatgcac
    54661 atattgtaaa agtttttccg cagcagatat ttccaaatca gttaactgtt catcaatcaa
    54721 atgaatatca ttaagtcgtt cgtcttcgta tgataaaagc aagttcatct gcttcgtcag
    54781 taggtgattt ttttcgtccg tcaattgata tcctactaca atctcacgag cttttaaatt
    54841 tagtatttca ctacggacgg actcaaaatc tgttaaaagg gttgcctgaa actctccagt
    54901 tgagacatcc atatatgcta gaccaaatgt ttgttgatct tgcgaatcaa tcgctactaa
    54961 gaaattatta gcactatccg gcttcgttga ctccacaacc gttcctgggg taacaacttg
    55021 cactacctca cgctttacca ctcccacagc ttttttagga tcttccatct gctcagcaat
    55081 ggctactttg taacctaatt caactaaaac gtcaatatac tgttgagctg agtgataggg
    55141 aacacctgcc attgggattg gcttttctgc gttcttattt cgactagtca agctaatctc
    55201 caggatttgt gctgctttta ccgcatcatc ataaaataat tcataaaaat cacccattct
    55261 aaaaagcaaa aaagcatctg gataattctc ttttatatcc agatactgtt gcattcccgg
    55321 tgatatcgtt ggcttggcca tttctcactc ctaactaacg gggctggctg ccatgctcag
    55381 ctcctcaatc attatattta taaacctact gatagttctt tttcaaaaac tttggcaata
    55441 tcttcatttt gacaaatcac caacaaggtg tctgctcctg ccattgttcc taaaatctct
    55501 ggcagccctt tctcatcaat aaagtttgct aaaacgctag cctcccctaa attagtattt
    55561 aaaactaaca taaaacttgc tcgcgatact ttcaaaatat aggagcgtaa ggctgggcta
    55621 aatcgatttg aagtagctgt tgataaacta taatatagct taccttctgg gctacgcaat
    55681 tttaaaagac caatctcacg caagtctctt gatagagtcg cttgcgtgac atcgattcct
    55741 tcatttttta aatgtaattt aatttcttct tgtgtgccaa tctgccctga ttggattaaa
    55801 cgtttaatac gtttttggcg ttctatttta ttcatgttat tcaatccttt ttagttagaa
    55861 ttatgcttac caaattatat caaaaaaaat tgtctttttc acttagtagt gatatcgtaa
    55921 tcattctgta aaaaagctat aaagtaggct agttctttac agaatgcatt atgaatttaa
    55981 tcaacaagtt ctcgtctatt tttcataatt atgttaaaat agggtgacta tattactaat
    56041 taaaatagga gataccatgg atacaaaaca tctaattgcg agtgaaattc aaaaagttgt
    56101 tccagatatg gaacaatcga ccattctttc tttattagaa accccaaaaa attcgagcat
    56161 gggagattta gctttcccag cattctcttt ggctaaaact ctacgcaaag cacctcaaat
    56221 aattgctagt gacattgctg aacaaattaa aagcgaccaa tttgaaaagg tggaagctgt
    56281 tggaccttac gttaactttt tccttgataa agccgcaatc tcaagtcaag ttctaaaaca
    56341 agtcttatct gatggttctg cctatgctac tcaaaatatt ggtgaaggac gaaatgttgc
    56401 cattgacatg tctagtccaa atattgctaa gccattctca attggacacc ttcgttcaac
    56461 agttattggt gatagtttag ctaatatttt tgataaaatt ggctatcatc ctgttaaaat
    56521 taatcacctt ggcgactggg gtaaacaatt tggaatgtta atcgttgctt ataaaaaatg
    56581 gggcaatgaa gaggctgtcc gtgctcatcc cattgatgaa cttttaaaac tttatgtccg
    56641 tattaatgct gaagccgaga cagaccctag cgttgatgaa gaggcacgtg agtggtttcg
    56701 taaacttgaa gctaacgatc ctgaagcaac tgaattatgg caatggttcc gtgacgagtc
    56761 attattagaa ttcaaccgtc tttacgataa aatgaatgtt acatttgata gctataacgg
    56821 cgaggcattc tacaacgaca aaatggagga agtgcttgaa ctgctagaat ctaaaaacct
    56881 cctggtcgaa tctaaaggtg cacaagttgt taacctagaa aaatacggta ttgaacatcc
    56941 tgctctcatt aagaaatccg atggagcaac tctctacatt acacgcgact tagctgctgc
    57001 actttaccgt aaacgtacct acgattttgc aaaatcaatc tacgttgtag gaaacgaaca
    57061 gtctgcacac tttaaacaac taaaagctgt tcttaaagaa atggactacg attggtccga
    57121 tgatatgact catgttcctt ttggccttgt aacaaaagga ggagcaaaat tatcaactcg
    57181 taaaggaaat gtaattctcc ttgaacctac tgttgcagaa gctattaatc gtgcggcatc
    57241 tcagattgaa gctaaaaacc ctaacctagc agataaagat aaagttgctc aagcagttgg
    57301 tgtaggtgct attaaatttt acgatttgaa aactgatcgt acaaatggtt atgactttga
    57361 cttagaagca atggtatcat ttgaaggtga aactgggcca tatgtacaat atgcacatgc
    57421 tcgtattcaa tcaatcttac gtaaagctaa ctttaaccca tctaatagcg ataattacag
    57481 tctcaacgat gttgaaagct gggaaatcat caaactcatc caagattttc ctcgtattat
    57541 tgtacgtgcg gcagataatt ttgagccctc tattattgca aaattcgcta tcaatttagc
    57601 tcagtgcttc aataaatatt acgcccacac acgtatttta gatgaagacg ctgaaatcag
    57661 tagtcgtcta gcactttgct atgcaactgc tacagtatta aaagaatctc ttcgcctcct
    57721 aggagttgat gctccgaatg aaatgtaaga aatgacacta ctaagactgt caaatttatg
    57781 acagtcttaa ttttttatat tataataata gcacaataag ttacaggaga atgccatgcc
    57841 atcagaaaaa gaaatattag atgctcttag taaagtttat agcgaagaag taatacaagc
    57901 agatgattat tttcgtcaag ctattttcga gctcgctagt caacttgaaa aagaggggat
    57961 gaactcactc ctagctacaa aaattgactc acttatcaat caatacgtct taacacatca
    58021 atttgatgct cctaaaagca tatttgatct tagtcgcttg gtaaaaacaa aagctagcca
    58081 ttacaaggga actgctatct cagctattat gcttggtagt ttcttaagtg gaggaccaaa
    58141 ataaaaatac aggccagaca gcctgtattt ttatttatcg taatttcttc gtcgaatttt
    58201 aaaatctgag ctaataactt catctacatt tataacagaa ataaaagcat ctgggtctaa
    58261 atcagccatg atttctttca catcacgcac ttcacttggg ttaagagcta cgtacaagac
    58321 atcatgctct ctacctgagt aggcaccttg gccccgtaaa aaagttacac ccctattaat
    58381 ttcatgcaaa atggttgctg ctgcagattc tgattctttg gtaataatga tcattccacg
    58441 aacagtgtag cccccatttt caacatttgt taacacttga ctaaatacaa agctagctac
    58501 taacgtatat agcatctgtt gtaaattaat ataaacaagt gaagacgtta gaactatagc
    58561 atcaataaac aataaggttt gccctaattt tattccagat ttttcctcca caatacgacc
    58621 aataatatct gcaccacctg tagttgcacc atatctaaat actaaaccac taccagttcc
    58681 tgctaaaatt ccagcagcta ctgcaactaa catcatatcg tttttcaaag ggactgttac
    58741 agggatttgt tgccaaaacc acatgaaaaa agacattaac actgtaccat atatagttaa
    58801 caataaggat tttttcccta aaatcctagc ccctaatata aataacggga tattaaggag
    58861 aagtgatgaa agtgctggat taactccgaa aagtgcatgg ataatcaagg tgacaccgga
    58921 aatacctcct tcagccaaat gattagccat attaaatttg acaaatccga aagtatagag
    58981 agccacaccc aaggcaatta aaatagcttc ttttatcttt gtttttaaat ccagtttcaa
    59041 cataggctta atccttcctt attcaaaaaa gctaaagttg tttaaacaac ttagcttaat
    59101 aacatcataa tgtttcctta gtggcagttg accaaaagtc atatatacag ttaatcatca
    59161 tcaacgaatc gtccaataat atgaacattt tctgcaacgg aaacaaaggc ttttggatct
    59221 gcttttagca ttaaatattt aaaatcacta aattcttcac gtgttaatat agttattaaa
    59281 actgcttttt tctcatgatt ataagtacct tcagcatcat tgatacaggt tacaccacga
    59341 tgtaaatcac gatggatacg tttaatgacg caatcaggct tactggtaac aatcatcgct
    59401 tgcatctttt tttgctttgt aaaaatggca tctgtaactc tacttgatac aaaaattgtc
    59461 accatcgagt acaaagcgta cttccaacca aataaaagac ctgcaaataa taaaataata
    59521 ccattaataa taaaagaaat actacctaca tctctgcctg ttttctttct aattgtcaaa
    59581 ctgataatat ctgttccccc actagaaatt ctagacttaa agctaaaacc aacaccagct
    59641 cccataatta agccaccaaa gatagcattg attaagggat cagttgtaag ggtgatttga
    59701 ggcattaatt gaatgaagat agagcttaca gttactgtga taaacgtaaa aatagtaaat
    59761 ttatgtccaa tcttacgcca agataatatc agcaaaggga tattaatagc ataaaaagct
    59821 aaagcaactg gtatttcaaa agaaaaccag tgtttactga cagctgaaat aacctgagct
    59881 aaaccagttg ctccacttga ataaacatgt ccaggttgaa aaaagaaatt gacagcaaca
    59941 ctggatagaa taccatacag taatgaagcc gaaattttct ctgcatattt ttctcgtgag
    60001 atacttttta aagtgtgtaa gagaccaaat tttttagccc atacactgat aatatatttt
    60061 accttttttt ctaaaggggt tcgtctagtt ttcataactt tctacagtaa gtgataattc
    60121 ttctaattgt tgttctgaaa cgagactagg agcttgagtc attggatcac tagccttatt
    60181 gtttttaggg aatgcaataa cttctcgaat attgtccttg cctgctaaca acataacaaa
    60241 gcggtcaagg cctattgcca atccaccatg tggtgggaag ccgtaatcca tagcttcaag
    60301 taagaaacca aactgttcct gagctgattc agcgctaaat cctaaagcct tgaacatacg
    60361 ctcttgtgta tctttttggt taatacgtag gctaccaccg cctaactcat atccattcaa
    60421 aacaatatca taggcaactg cacgtacttt agccaaatcc ccttccaact catgcgctgt
    60481 ctccgctgtt ggcaaggtga atggatgatg agccgacata tatcgtccct cttcttctga
    60541 ccactcaaac attggccaat caacaatcca taggaaatta aatttagaat aatcaatgag
    60601 ttcaagttct tttgcaatgc gtgtacgaag cgctcctaaa gtctcgttag ctacttcaag
    60661 actatctgct acaaatagaa ttaagtcatt attctctagc tgtaaagctt ctgtcaacct
    60721 accttcaata gctgtcaaga acttagcaac cggacctgaa atagtattat ctgcatattt
    60781 tagccatgct aaacctttgg caccgtattg ttttgcaatt tctgttaatt tatcaatatt
    60841 ttttcgagag tacttgtccg ccttattttt gacaacaatc gctttaacta ctgaagcttc
    60901 tgagaatacc ttgaagtcaa cttcttttac aacttctgtc aagtcttgta aaagcatatc
    60961 aaaccttgta tcaggcttat cagacccgta gttattcatt gcatcatcat atgccatcct
    61021 tgggaatggt aaagaaacct ctaaaccttt agtgtctttc attactttag caatcatacc
    61081 ctcaacaatg tcttgaattt cttgatcact aaggaatgat gtctctaagt caacttgtgt
    61141 aaactcgggt tgacgatctc cacgtaaatc ctcatcacgg aaacatttga caatttgata
    61201 ataacgatca aatcccgcat tcatcaatag ctgttttgta atttgagggc tttgtggaag
    61261 ggcataaaaa tgtccttggt tgacacgtga tggaaccaag tagtctcgtg ccccctctgg
    61321 agttgatttt gttagcatcg gagtttcgac atcaataaac tctaagttgt caaggtaatt
    61381 acggattgag tgtgttactt tcgcacgtaa tttgaaattt tcaagcattt ccggacgacg
    61441 aagatccaag taacggtaac gcatgcgtgt atcatcattt gcctcaatac catctttaat
    61501 ttcaaatggt gttgttttcg aggtattaag aaccgataat tcttgcactt ttaattcaac
    61561 ttctcctgtt ggtaaattat cattagcttg ttctcgtgct gtcactacac cgctaacctc
    61621 tataacaaat tcactacgga gactttctgc ggttgccata actgaggctg atacttcttc
    61681 aggattaata actaattgca tgattccctc gcgatcgcga agatcaataa aaataagacc
    61741 tccaaggtca cgacgacgac caacccatcc ttttaaagta atcgatgttc ctatgtgctc
    61801 actacgaaca cgcccagcat acatactacg tttcatctgt tttctccatt taaaatattc
    61861 attccaacct attataacaa aaagcaccta aaaaccccac ccattaagga gagtttttag
    61921 gaactgtttt acaatcctaa ttgtttaagc acagatgaaa aacctgtttt aatgttttca
    61981 aaactaactt taacttcttg acgtgtttga ttatttttta aactgacctc tctcgaatct
    62041 acttcactgc tacctaatgt cataatcact ttagcattaa aagtatctgc agacttaaat
    62101 tgtgctttga ttttacgacc caaataatca cgttctactt taaaaccttg atgacgtatt
    62161 gattgtgcca aatctaaggc agctagatta gcctctgatc ctaatacagc tatatacaga
    62221 tctatagtat tttctattgg taaaggaatc ccttgcttac ctaatattaa gagcaagcgt
    62281 tctaaaccta atccaaaacc aaatgctgga gtttctggtc caccaaaata ggatactaag
    62341 ccgtcataac ggcctccagc acagatcgtt aattcattat cttcaatctc agtgataaac
    62401 tcaaaaatag tatgattata ataatcaaga ccacgaacca tattagtatc aatgatgtat
    62461 ggaatattta gagcatcaag catatgacac acagcatcaa agtgggcttg gcttgactca
    62521 tctaaataat ctaaaataga aggagcattt tcaactgcta gtttgtcttc tggctcctta
    62581 gagtctagta cgcgcaatgg attttcattc aaacgacgtt gactatcctt agacaattgt
    62641 tcacgtatgg gtgttaagta atcaataaga gcttggcgat aggcttgacg actttctgga
    62701 ctccctaaac tattaagatg aagtttaaca tttttgatac ctaaatcttc aaatagatga
    62761 tgtcccattg cgattgtttc aacatcagtt gctggattat ttgatccaaa gcactcaacg
    62821 cctacttgat gaaattctcg taaacgacca gcctgtgggc gctcatatcg gaacattgat
    62881 ccaatataat acattttggt tggtttttga acttctggtg caaatagttt attttcaaca
    62941 taagaacgta caacaggtgc tgttccttct ggacgaagcg taatgtgacg atctccttta
    63001 tcatgaaaat catacatctc ttttgtaacg atatccgttg tatccccaac tgagcgacta
    63061 ataacctcgt aatgttcaaa cattggtgta cgaatttcat cataatgata ttgtttaaag
    63121 agattgcgta taacattttc aacgtattgc cactttgctg attctccagg taagatatcc
    63181 tgagtccctt ttggtttttg aagtttcata agtttacctc ttcgattagt tttctcttta
    63241 aacattctac cattttttct tatattttaa acaagcatat atcatttgct ttagtttgat
    63301 agtattttca ccatgaaatt gtaaaaataa aaagttttgt caagtaactt tactttacaa
    63361 aaaagatatg ttatcatagt gaagttgatg aaaatcaaaa cttacacatc gaaaggaaga
    63421 ataagaaaat ggcagtacct gcacgtcaca cttcaaaagc gaagaaaaac aaacgtcgta
    63481 cacactacaa attgacagct ccatctgtac aattcgacga aactactgga gattactcac
    63541 gttcacaccg tgtatcactc aaaggatact acaaaggacg taaaatcgct aaagctaacg
    63601 aagctaaata atagaaggga gataccatgc gcgtaaatat tacacttgaa cataaagaat
    63661 ctggtgaacg cttgtacctt acttcaaaaa acaaacgtaa cactccagac cgtcttcaat
    63721 tgaaaaaata ctcaccaaaa cttcgtaaac acgttgtttt tactgaggtt aagtaagggt
    63781 ttcagtacac gtcaatagac gtcaaaatat tgattttaag cgatttttga ttttttgaaa
    63841 ctcattatat cgcactacaa atcaataaaa actctacctt ttactctacc ttttttagat
    63901 aaatatatca tctggatatt gaagttaagc cctgctatca tttcgatggc agggcttttt
    63961 aatatttggt acactaactt attatctata ttttggtgga ttttaagttg acatctacaa
    64021 agtttaatgt tagaatacat tcaaaaatat atttgaatga ggtattttat gattcaaaat
    64081 gttgttactt caataatcct gtattctggg acagccgtag acttacttat tatcctaatg
    64141 ttattttttg ccaaaagaaa aagcagaaaa gacatcatta acatctattt aggacaattt
    64201 ctaggctctg ttagtctaat attcctaagt ttgctttttg catttgtctt aaattatatt
    64261 cctagtaaag agattttagg tttactcggt ttgattccaa ttttcctagg cctaaaagtt
    64321 ttgcttttag gagattctga tggagaagct attgcaaaag atggtttgcg aaaagacaat
    64381 aaaaacctga tttttctagt cgctatgatt acttttgcaa gttgtggcgc tgacaatatt
    64441 ggtgtctttg tcccatattt taccacctta aatttagcga atttgatagt gactttactt
    64501 acttttctag tcatgattta tctcttggtt ttttctgcac aaaaattagc acaagtccct
    64561 tctgttggag aaactttgga aaaatatagc agatggttta ttgccgttgt ctatttagga
    64621 ttggggatgt atatcctgat tgaaaacaac agctttgaca tgctatgggc tgtgttaggc
    64681 taggagaaaa aattatgaaa aaagatagta tctgtcaagt ggatgttata aatcaacaaa
    64741 atgttacaac cgcaacgaac taccttgaaa aggaaaaagt ccaaaaatca cttcgcattt
    64801 tatcaaaatt taccgataat aaacagataa atatcatctt ttatctcctt gccgtcgaag
    64861 aactctgtgt ctgcgatata gcctgtttat taaatctcag tatggcatct gcctcccacc
    64921 atcttcgtaa actagccaat caaaacatct tggacactag aagagagggg aaaattgtat
    64981 attattttat aaaagatgag gaaatcagag atttttttaa tcaactagga taacaactat
    65041 ttc
//
LOCUS       DATRNK010000005        60566 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.005, whole genome shotgun sequence.
ACCESSION   DATRNK010000005 DATRNK010000000
VERSION     DATRNK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 60566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60566
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.005"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(80..949)
                     /gene="rsmI"
                     /locus_tag="VBC52_000300"
     CDS             complement(80..949)
                     /gene="rsmI"
                     /locus_tag="VBC52_000300"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEO6881907.1"
                     /translation="MEMQVQKSFKSNTHYGTLYLVPTPIGNLDDMTFRAIRILREVDF
                     ICAEDTRNTGLLLKHFDITTKQISFHEHNAYDKISGLIDLLKEGRSLAQVSDAGMPSI
                     SDPGHDLVKAAIEGDIPVVSIPGASAGITALIASGLASQPHIFYGFLPRKQGQQITFF
                     ETKKDYPETQIFYESPFRVSDTLKHMKEIYGDRQVVLVRELTKLYEEYQRGTISQLLG
                     HIEKVPLKGECLIIVDGKRDTERVKDSSQQDPLVLVKEYIANGDKTNQAIKKVAKELN
                     LNRQELYASFHDL"
     gene            complement(949..1275)
                     /gene="yabA"
                     /locus_tag="VBC52_000301"
     CDS             complement(949..1275)
                     /gene="yabA"
                     /locus_tag="VBC52_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEO6881908.1"
                     /translation="MDKKDLFDAFDDFSQNLLVGLSEIETMKKQIQKLLEENTVLRIE
                     NGKLRERLSVIEAETETAVKNSKQGRELLEGIYNDGFHICNTFYGQRRENDEECAFCI
                     ELLYRD"
     gene            complement(1306..2169)
                     /locus_tag="VBC52_000302"
     CDS             complement(1306..2169)
                     /locus_tag="VBC52_000302"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEO6881909.1"
                     /translation="MDLKRTQPKLLEKFNTILQSDRMSHAYLFSGNFASLDMALYLAQ
                     SQFCEKRQSGLPCQKCRACRLIANGEFSDVKIIEPQGQLIKTETIKELTKDFSRSGFE
                     GKSQVFIIKDCEKMHVNAANSLLKFIEEPQSSSYVILLTNDENNVLPTIKSRTQIFRF
                     PKQLDMLVHQAEQAGLLKSQASLLAQVADDPKHLEILLANKKLLDYLNLSQQFVTTLA
                     KDRQTAYLEVSRLTSQVVDKNDQAFVFQWLTIMLAKEGQLYDLENTYRAQQMWKSNVS
                     FQNSLEYMVLS"
     gene            complement(2189..2824)
                     /locus_tag="VBC52_000303"
     CDS             complement(2189..2824)
                     /locus_tag="VBC52_000303"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEO6881910.1"
                     /translation="MKKGLMISFEGPDGAGKTTVLEAVVPLLREKLSQDILTTREPGG
                     VTISEEIRHIILDVKHTQMDKKTELLLYMAARRQHLVEKVLPALEEGKIVLMDRFIDS
                     SVAYQGSGRGLDKSHIKWLNDYATDSHKPDLTLYFDVPSEVGLERIQKSVQREINRLD
                     LEQLDMHQRVRQGYLELADLEPNRIVTIDASQQLDEVIAETFSIILDRINQ"
     gene            complement(2913..3572)
                     /locus_tag="VBC52_000304"
     CDS             complement(2913..3572)
                     /locus_tag="VBC52_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6881911.1"
                     /translation="MPVKDFMTKKLVYVSPDTTVAEAADLLREHHLRRLPVVENDQLV
                     GLVTEGTMAEAQPSKATSLSIYEMNYLLNKTKIRDIMIKDIVTVSQYASLEDAIYLMM
                     SRKIGVLPVVDNGQLYGIVTDRDVFKAFLEIAGYGQEGYRLVILADEGIGVLSKVLNR
                     LSSANLSVKRLVIIERKAGKKAVEIQLEGYADKDVLKQELVFDDVIVETLEKTVKKPL
                     S"
     gene            complement(3591..4301)
                     /locus_tag="VBC52_000305"
     CDS             complement(3591..4301)
                     /locus_tag="VBC52_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6881912.1"
                     /translation="MTMLKVENLSIHYGVIQAVNDVSFEVNQGEVITLIGANGAGKTS
                     ILRTISGLVRPSQASISFMGKPIHKLAARKIVGNGLAQVPEGRHVFSSLSVMENLEMG
                     AFLQKDREQNQKMLKKVFDRFPRLEERKNQDAATLSGGEQQMLAMGRALMSRPKLLLL
                     DEPSMGLAPIFIQEIFNIIEDIKKQGTTVLLVEQNANKALTIADKAYVLETGKVVLSG
                     TGKELLVSDQVRKAYLGG"
     gene            complement(4301..5065)
                     /locus_tag="VBC52_000306"
     CDS             complement(4301..5065)
                     /locus_tag="VBC52_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6881913.1"
                     /translation="MALLEVKNLSKHFGGLTAVGDVSMKLHKGELIGLIGPNGAGKTT
                     LFNLLTGVYLPSKGTISLDGKILNGRKPAKIASLGLGRTFQNIRLFKNMTVLDNVLVG
                     LSNHHLSHPIASFLRLPKYYHSEKALRKKALELLEIFGLKAYQDALAKNLPYGKQRRL
                     EIVRALVTEPKILFLDEPAAGMNPQETAELTQLISQIKSDFDITIMLIEHDMNLVMQV
                     TERIYVLEYGRLIAHGTPEEIKNNKRVIEAYLGGEL"
     gene            complement(5066..6019)
                     /locus_tag="VBC52_000307"
     CDS             complement(5066..6019)
                     /locus_tag="VBC52_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO6881914.1"
                     /translation="MKENMKSILSWLAIVVLGYLLISVLISMGIFNLYHIQIIETIGI
                     NVILAVGLNLIVGCSGQFSLGHAGFMAIGAYAVAIIGVKMPTYVGFLIAILVGTLVAG
                     GIALGVGIPTLRLKGDYLAIATLGVAEIIRILLVNGGDITNGAAGIMGIPPFTTWSLV
                     YGVAVVSLILAMNFLRSPLGRNTIAIREDEIAAESMGVDTTKVKVIVFIFGAILASIA
                     GSLQAGYVGTVMPKDFSFMMSVNVLIIVVLGGLGSMTGTVLAAILLGLLNMLLQDYAS
                     VRMIIYALALILIMIFRPSGLLGTKELTLSHLFRKTSKGGI"
     gene            complement(6022..6891)
                     /locus_tag="VBC52_000308"
     CDS             complement(6022..6891)
                     /locus_tag="VBC52_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO6881915.1"
                     /translation="MLQQLINGLILGSIYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FMGYYLINHLHLNFFLALLIAMLGSAFLGAVIEYLAYRPLRKSTRIAALITAIGVSFL
                     LEYGMVYLVGADTRAFPQAIHTVKYNLGPITITNVQLIILGIALLLMLTLQFIVQKTK
                     MGKAMRALSVDSDAAQLMGINVNRTISFTFALGSALAGAGGVLIGLYYNSVQPLMGVT
                     PGLKAFVAAVLGGIGIIPGAAIGGFVIGILETLATALGVSDFRDGIVYAILILIFLIR
                     PAGILGKNIKEKV"
     gene            complement(6997..8163)
                     /locus_tag="VBC52_000309"
     CDS             complement(6997..8163)
                     /locus_tag="VBC52_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6881916.1"
                     /translation="MEKRLSLGALVLASTVLLAACGNVGGGASSTGTKIGKDIKVGYN
                     WELSGNVSSYGNSMKNGADLAVKEINAAGGVGGKKLKVLSQDNKSENAEAATVATNLV
                     TKGANVIIGPATSGAAASSTPKVNAAAVPMIAPAATQDNLVYGSDGKTLNQYFFRATF
                     VDNYQGKLLSQYATDNLKAKKVVLFYDNSSDYSKGVAKSFKESYSGKIVDSMTFSAGD
                     TDFQASLTKLKGKEYDAIVMPGYYTETGLIVKQARDLGISKPVLGPDGFDSPKFVQSA
                     TPVGASNVYYLTGFTTQGSTKAKAFHDHYVKAYGEEPSMFSALSYDAVYMAAKSAKGA
                     KTSIDLKKALAKLKDFKGVTGKMSIDKNHNVVKSAYVVKLDDGKTSSVNIISAK"
     gene            complement(8317..8562)
                     /locus_tag="VBC52_000310"
     CDS             complement(8317..8562)
                     /locus_tag="VBC52_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2129 domain-containing protein"
                     /protein_id="HEO6881917.1"
                     /translation="MNKQERLGMSVYLYYNRDARKLYKYGDVHYHSRRLRYLVIYVNK
                     EDIVSVSKEIKHLKFVKDVRLSAIDDIDQDFVGNLYR"
     gene            complement(8662..9252)
                     /locus_tag="VBC52_000311"
     CDS             complement(8662..9252)
                     /locus_tag="VBC52_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEO6881918.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGQVEDNMANSIIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKSDVQTIVMGMAASMGTIIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQSDMAIAAEHLLKTRHTLEKILADNSGQ
                     SIEKVHDDAERDRWMSAQETLDYGFIDAIMENNNLQ"
     gene            complement(9377..10006)
                     /gene="upp"
                     /locus_tag="VBC52_000312"
     CDS             complement(9377..10006)
                     /gene="upp"
                     /locus_tag="VBC52_000312"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEO6881919.1"
                     /translation="MGKFQVISHPLIQHKLSILRRTTTSTKDFRELVDEIAMLMGYEV
                     SRDLPLEDVEIQTPVATTVQKQLAGKKLAIVPILRAGIGMVDGFLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGAASIKFVCL
                     VAAPEGVAALQEAHPDVDIYTAALDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(10117..11286)
                     /locus_tag="VBC52_000313"
     CDS             complement(10117..11286)
                     /locus_tag="VBC52_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6881920.1"
                     /translation="MITYDFTSLPERFSSNAIKWKAVQKDQEILPLWIADMDFPIFPE
                     MSEAIEDFSHQMVFGYDSPKDSLYQAISNWEVQEHGYQFDKKSLLLIDGVIPAISVAI
                     QAFTKEGDAVLINTPVYPPFARTIKYNNRHLVSNSLLNNNQYFEIDVKQLEKDIIENN
                     VKLYIFCSPHNPGGRVWTKGEIQKIGDICKRYNVILVSDEIHQDLVLFDNVHHSFNTV
                     DSTFKELSVILSSATKTFNIAGTKNSFAIIENEKLRSDFKKRQIANNQQEISSLGLLA
                     TEVAFTKGKQWLKALKMELEGSIEYLYEQLTQKTHIKVMKPEGTYLVWLDFSAYNLTH
                     LEIQEKLRYDAKLILNDGLTFGKEGKKHARINVAAPRSVIEEAVLRLVTNFRKND"
     gene            complement(11422..11970)
                     /locus_tag="VBC52_000314"
     CDS             complement(11422..11970)
                     /locus_tag="VBC52_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEO6881921.1"
                     /translation="MNIETLTQKNHRSDSGRNHIVLFEPQIPANTGNIARTCAATNAP
                     LHIIRPMGFPIDDKKMKRAGLDYWDKLDVSFYDSLEEFMLSCRGKVHLISKFADKVYS
                     DENYNDDQDHYFMFGREDKGLPETFMREHAEKALRIPMNDEHVRSLNVSNTVCMIVYE
                     ALRQQSFPNLELSHTYENDKLK"
     gene            complement(12143..13495)
                     /locus_tag="VBC52_000315"
     CDS             complement(12143..13495)
                     /locus_tag="VBC52_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEO6881922.1"
                     /translation="MENHNSIKQTYGLMTTIAMIVGVVIGSGIYFKVDDILKFTGGDV
                     FLGMVILVLGSFSIVFGSLSISELAIRTSESGGIFSYYEKYVSPALAATLGLFASFLY
                     LPTLTAIVSWVAAFYTLGESSSLESQIILAAVYILALSLMNIFAKRIAGGFQSLTTFV
                     KMIPLVLIALIGAFWSDKAPQLPQHLTAIQPSNVGWSWVSGLVPLYFAYDGWTIFVSI
                     APEVKNPKKNLPLAFVIGPALILLSYLAFFYGLTQILGASFIMTTGNDAINYAANIIF
                     GPSVGRLLSFIVILSVLGVANGLLLGTMRLPQAFAERGWIKSERMANINLKYQMSLPA
                     SLTVTAVAIFWLFVHFMVTKFNLLPGSDISEIAVVFNNTSLIILYVLVLSLYLKKDIK
                     NKFTGLVSPILAILGGLILFIGSLLSNFFTVLIFQCFCLLFCLICHYIYQKNNPKTHE
                     "
     gene            13701..15050
                     /gene="trkA"
                     /locus_tag="VBC52_000316"
     CDS             13701..15050
                     /gene="trkA"
                     /locus_tag="VBC52_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEO6881923.1"
                     /translation="MRIIVVGGGKVGTALCRSLVAEKHDVVLIEKKENVLKRVTKQHD
                     IMGIVGNGANYKILEQAEVKNCDIFIAITDRDEVNMISAVLAKKMGAKETVVRMRNPE
                     YSNPYFKDKNFLGFSSVVNPELLAAQYIANTIEFPNATSVEHFANGRVMLMEFKILEG
                     NKLCHTSMSQIRKKFGNIVICAIERDGKLIIPDGDATIQVKDKIFVTGNRIEMILFHN
                     YVKNKVVKNLMVIGAGRIAYYLLNILKNTNTHVKLVELNQEQAEYFSQEFPNVPVVHG
                     DGTAKNILLEESVTSFDAVATLTGVDEENIITSMFLESIGIPKNITKVNRTSLLEIID
                     DKQLSSIITPKRIAVDHVMHFVRGRVNAQDSNLEAMHHIANDRIETLQFEIKETSKLA
                     NRSLASLKLKQNILIAAIIRNNKTIFPTGEDVLTVGDRIVVITLLKNITRTSDMLAR"
     gene            15055..16494
                     /locus_tag="VBC52_000317"
     CDS             15055..16494
                     /locus_tag="VBC52_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO6881924.1"
                     /translation="MNKSMIRFLLSKLLMIEAALLAIPLTVGLIYREPQSVMMSIVIT
                     MIILIILGLLGSLFKPKNYHIYTKEGMLIVALCWILWSFFGALPFVISGQIPNIIDAF
                     FEVSSGFTTTGATILDDVSVLSPALLFWRSFTHLIGGMGVLVFALAIMENSKNSHLEV
                     MRAEVPGPVFGKVVSKLKKTAQILYLLYLLMFAVFAVILYFAGMPFFDSIIIAMGTAG
                     TGGFAVYNDSIAHYNSPLITNLVSIGMLIFGVNFNLYYLLLLRKIKAFFGDEELKTYL
                     RIVAIATFMIALNVIGMYDNFRQGLEHIFFEVSAIITTTGFGVTDITRWPLFSQVILL
                     FLMFIGGSAGSTAGGFKVMRSLILAKIARNQVLSTLYPNRVMSLHINKSVLDKNTQHG
                     VLKYLTIYLAIFMALVLVLTLDTNDFLVVVSAAASCFNNIGPLLGSNETFSFFSPFSK
                     LLLSFAMIAGRLEIYPVLLMFIPKTWSKL"
     gene            complement(16518..16769)
                     /gene="yidD"
                     /locus_tag="VBC52_000318"
     CDS             complement(16518..16769)
                     /gene="yidD"
                     /locus_tag="VBC52_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEO6881925.1"
                     /translation="MLKSFLIFLVRFYQKNISPAFPASCRYRPTCSTYMIEAIQKHGL
                     KGVLMGIARILRCHPLAHGGNDPVPDHFSLRRNKTDISD"
     gene            complement(16769..17491)
                     /locus_tag="VBC52_000319"
     CDS             complement(16769..17491)
                     /locus_tag="VBC52_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6881926.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGMVTINGQVVNELATQVKAG
                     DLVEIEGSPIYNEEKVYYLLNKPRGVISSVSDDKGRKTVIDLLPQVKERIYPVGRLDW
                     DTTGLLILTNDGDFTDKMIHPRNEIDKVYLARVKGIATKENLRPLTRGVVIDGKKTKP
                     ARYTIIKVDHEKNRSVVELTIHEGRNHQVKKMFEQVGLLVDKLSRTQFGTLDLTGLRP
                     GEARRLNKKEISQLHNAAINKS"
     gene            complement(17481..18065)
                     /locus_tag="VBC52_000320"
     CDS             complement(17481..18065)
                     /locus_tag="VBC52_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEO6881927.1"
                     /translation="MTYLGSIEALLFVAGEDGLSLRQMAELLSLTPSALIQQLEKLAK
                     RYEEDDDSSLLLLETAQTYKLVTKDSYMTLLRDYAKAPINQSLSRASLEVLSIIAYKQ
                     PITRIEIDDIRGVNSSGAITRLIAFGLIKEAGKKEVLGRPNLYETTNYFLDYMGINQL
                     DDLIDASSIELVDEEVSLFSMDSINTEDKENNEN"
     gene            complement(18062..18769)
                     /locus_tag="VBC52_000321"
     CDS             complement(18062..18769)
                     /locus_tag="VBC52_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEO6881928.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYEVDIYDVPIVEVIEQYLAYIAT
                     LQAMRLEVAGEYMLMASQLMLIKSRNLLPKVVESNPIEDDPEMELLSQLEEYRRFKVL
                     SEELANQHQERAKYFSKPKQEVIFEDAILLHDKSVMDLFLTFSQMMSQKQKELSNSQT
                     VIEKEDYRIEDMMIVIERHFNLKKKTTLQEVFADCQTKSEMITLFLAMLELIKLHQIT
                     VEQDSNFSQVILRKEEK"
     gene            complement(18769..19509)
                     /gene="xerD"
                     /locus_tag="VBC52_000322"
     CDS             complement(18769..19509)
                     /gene="xerD"
                     /locus_tag="VBC52_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEO6881929.1"
                     /translation="MINDINNFIESKKLSLNSRKSYHYDLKQFYKIIGGHVNSEKLAL
                     YQQSLSEFKLTARKRKLSAVNQFLFFLYNRGTLKEFYRLQETEKITLAQTKSQIMDLS
                     NFYQDTDYPSGRLIALLILSLGLTPAEIANLKKADFDTTFNILSIEKSQMKRILKLPE
                     DLLPFLLESLEEDGDLVFEHNGKPYSRQWFFNQLTDFLNEKNEQQLTAQLLREQFILK
                     QKENGKTMTELSRLLGLKTPITLERYYR"
     gene            complement(19499..19972)
                     /locus_tag="VBC52_000323"
     CDS             complement(19499..19972)
                     /locus_tag="VBC52_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6881930.1"
                     /translation="MIAKEFESFLLSHLDHYLIPAEDVAIFVDTHNADHVMLLLASNG
                     FSRVPVITKEKKYVGTISISDIMGYQSKGQLTDWEMAQTDIVEMVNTKIEPINEAATL
                     TAIMHKIVDYPFLPVISDQNDFRGIITRKSILKAINSLLHDFTDEYTITPKNNDK"
     gene            complement(19969..20490)
                     /locus_tag="VBC52_000324"
     CDS             complement(19969..20490)
                     /locus_tag="VBC52_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO6881931.1"
                     /translation="MAIRKLVVMSDSHGDRDIVKDIKNHYLGKVDVIFHNGDSELPSS
                     DPIWEGIHVVTGNCDYDSGYPEVLVTKIDNTVIVQTHGHLHQINFTWDKLDLLAQQED
                     ADICLYGHLHRADAWKNGKTIFINPGSVLQPRGPINEKLYAVVTITDSKVLVEYYTRQ
                     HQPYPNLTKEFSR"
     gene            complement(20472..21446)
                     /locus_tag="VBC52_000325"
     CDS             complement(20472..21446)
                     /locus_tag="VBC52_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEO6881932.1"
                     /translation="MTKTIFESKTEGNWFLGSFQAFNYFTCFGNDESYEAIQDVFHRL
                     LSTLKVEGLQLHVVQMTSDFQLLAFLVDMINQEYSRHIKVTQHKGAILVSEDDQLFLV
                     HLPKEGTSLEKFFDLKNDNNFGDTILIATHNEGKTKEFRELFGKLGLKVENLNDYPDL
                     PEVEETGMTFEENARLKAETISKLTGKMVISDDSGLKVDALGGLPGVWSARFSGPDAT
                     DARNNAKLLHELAMVFDKERRSAQFHTTLVVSAPNKESLVVEAEWPGYIGTEPKGENG
                     FGYDPLFIVGEGSRTAAELSAQEKNNLSHRGQAVRKLMEVFPKWQLEN"
     gene            complement(21443..22237)
                     /gene="racE"
                     /locus_tag="VBC52_000326"
     CDS             complement(21443..22237)
                     /gene="racE"
                     /locus_tag="VBC52_000326"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEO6881933.1"
                     /translation="MDSRPIGFLDSGVGGLTVVKEMFRQLPEEEVIFIGDQARAPYGP
                     RPAQQIREFTWQMVNFLLTKNVKMIVIACNTATAVAWQEIKEKLDIPVLGVILPGASA
                     AIKSTNSGKVGIIGTPMTVKSDAYRQKIQALSPNTAVVSLACPKFVPIVESNQMSSSL
                     AKKVVYETLSPLVGKLDTLILGCTHYPLLRPIIQNVMGAEVKLIDSGAETVRDISVLL
                     NYFEINHNWQNKHGGHHFYTTASPKGFKEIAEQWLSQEINVERIVL"
     gene            complement(22412..22651)
                     /locus_tag="VBC52_000327"
     CDS             complement(22412..22651)
                     /locus_tag="VBC52_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEO6881934.1"
                     /translation="MSTTIWILLIIVALFGGLVGGIFIARKQIEKEIGEHPRLTPDAI
                     REMMSQMGQKPSEAKVQQTYRNIVKHAKTAIKTKK"
     gene            complement(22809..23351)
                     /locus_tag="VBC52_000328"
     CDS             complement(22809..23351)
                     /locus_tag="VBC52_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881935.1"
                     /translation="MSKKTTQMVSYTSILVAFAIMIPIIMPAKIIIGPASFTLASHVP
                     LFLSIFISVPVAILVALGTGLGFLLAGFPIVIVLRALSHIGFALIAAFLIKSKPSLLM
                     SKWQTLLFAVAINIIHGLLEFITVYIITMTSNSSSTYLWSLFSLIGLGSLLHGLVDFY
                     IALFIWKWMTQKLGKTLRIR"
     gene            complement(23413..23934)
                     /locus_tag="VBC52_000329"
     CDS             complement(23413..23934)
                     /locus_tag="VBC52_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEO6881936.1"
                     /translation="MKAQERRENILTTLKGTKEAISASTLAKIFSVSRQVIVGDIALL
                     RAQQCDIISTPKGYLMSSALSTHQFTARLVCQHGIEQTEEELEIILRYQGIIMNVEVE
                     HPIYGMLTAPLNIQSQKDIDNFTAKLKVSNAELLSSLTDGLHTHMISCQDQSVFDQIC
                     EALKKAGILYIDK"
     gene            complement(24002..24691)
                     /locus_tag="VBC52_000330"
     CDS             complement(24002..24691)
                     /locus_tag="VBC52_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEO6881937.1"
                     /translation="MNDNVIYTQSDSGLNQFFAKIYGLVGIGVGLSAAVSAIMLYMFP
                     QNMIAIMQKMPGLYFGAIILELVLVFVASGAARRNTPAALPLFLIYSALNGFTLSFII
                     ARYTQTTVLQAFITSAAVFFAMALIGAKTKKDLSGMRKALMAALIGILIASLVNLFIG
                     SGGMSYIISIVCVIIFSGLIAYDNQMIKYVYNSQGGQVADGWAVSMALSLYLDFINLF
                     LNILRLFARND"
     gene            complement(24714..25217)
                     /locus_tag="VBC52_000331"
     CDS             complement(24714..25217)
                     /locus_tag="VBC52_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6881938.1"
                     /translation="MKKYQDDKDFMDLVGHLIDHPRFQKLEAIVQHHHSTRLEHSINV
                     SYTSYKIAKKFGWDASSTARGGLLHDFFYYDWRVTKFNKSHAWVHPRIAVRNARKLTD
                     LNAREEDIILKHMWGATIAPPRYKESYIVTMVDKYWAVREASRPLKRIFKKPIRFSRK
                     FLGSHNH"
     gene            complement(25255..25998)
                     /locus_tag="VBC52_000332"
     CDS             complement(25255..25998)
                     /locus_tag="VBC52_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEO6881939.1"
                     /translation="MTTIITSKSNNLIKKTKKLLQKKYRKSSYLIEGWHLFEEAEKYG
                     AQFLNIFVTETAIDRLRKPERAIVVTDDILKELTDSQTPQGIVAEIAFQETRWTDIKK
                     GRFLVLEDVQDPGNLGTMVRTADAANFDAVFLSQKSADLYNQKTLRSMQGSHFHLPVF
                     RVEIEQFVNFCKAEGITMIATTLSEQSVNYKNLPKYDYFALIMGNEGQGISKTMTEEA
                     DVLAHIEMPGQAESLNVAVAAGVVIFSLI"
     gene            26030..26308
                     /locus_tag="VBC52_000333"
     CDS             26030..26308
                     /locus_tag="VBC52_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEO6881940.1"
                     /translation="MKKVHLIVSGRVQGVGFRYATYFLALEIGDIYGRVWNNDDGTVE
                     ILAQSTDSNKMTQFIQKIRKGPSKWSKVTYVDIKLDNFDDFNDFKMRN"
     gene            26393..27325
                     /gene="yidC"
                     /locus_tag="VBC52_000334"
     CDS             26393..27325
                     /gene="yidC"
                     /locus_tag="VBC52_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO6881941.1"
                     /translation="MKKTLKRILFSSLSLSMLLLLTGCVSVDKAGKPYGVIWNTLGVP
                     MANLITYFAQHQGLGFGVAIIIVTIIVRVVILPLGLYQSWKASYQAEKMAYFKPLFEP
                     INERLRNAKTQEEKLAAQTELMTAQRENGLSMFGGIGCLPLLIQMPFFSAIFFAARYT
                     PGVSSATFLGLNLGQKSLTLTVIIAILYFVQSWLSMQGVPDEQRQQMKTMMYLMPIMM
                     VFMSISLPASVALYWFIGGIFSIIQQLVTTYVLKPKLRRKVEEEYTKNPPKAYKANNA
                     RKDVTNSTKATESNQAIITSKKTNRNAGKQKRRG"
     gene            complement(27525..28190)
                     /locus_tag="VBC52_000335"
     CDS             complement(27525..28190)
                     /locus_tag="VBC52_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6881942.1"
                     /translation="MINWDAIFNLELAVKAFPSVIQGLPYTIGLSLVGFILGAIVGFF
                     VALMKMSHFRLLRYLANIHISLMRGIPLMVLLFLIYFGLPFIGIQLDAVTASIVGFTM
                     MSSAYISEIIRAALLAVDHGQWEAARALGLKTPTIYRGIIIPQATRIALPSLSNVLLD
                     MVKGSSLTAMITVPDIFNNAKIVGGTYSDYMTAYILVALIYWVICTLYAIIQDWWEKR
                     LLY"
     gene            complement(28214..29071)
                     /locus_tag="VBC52_000336"
     CDS             complement(28214..29071)
                     /locus_tag="VBC52_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6881943.1"
                     /translation="MKKTILLGLVGLSAMTLAACSNGQSSKETTWDNIKKDGVLKVAT
                     PATLYPTSYYDDHKKLTGYEIDMMKAIAKKLKIKVKFVEVGVAESFTSVDSGKVDVAV
                     NNFDTTPERLKKYNFSQPYKYSVGGMIVRADGSSKITAKDLSDWKGKKAGGGAGTQYM
                     KIAKQQGAEPVIYDNVTNDVYLRDVSTGRTDFIPNDYYTQVIAVKYVTKQYPDIKVKM
                     GDVKYNPTEQGIVMSKKDKSLKTKIDAAIKDMKKDGSLKKISEKYYAGQDLTKEPAGT
                     DKIPVIKVK"
     gene            complement(29232..30692)
                     /locus_tag="VBC52_000337"
     CDS             complement(29232..30692)
                     /locus_tag="VBC52_000337"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEO6881944.1"
                     /translation="MVFKDATAMVQAIKQHKISSQELVEQAIYKIEEQNVSVNAVVSK
                     QYNEARQAVKYANESNAPFAGVPILLKDLGQNQKGQLSTSGSQLFKHYHAKQTDYLVQ
                     SFEKLGFIILGRTNTPEFGFKNISDGQLHGNVNLPFDHSRNAGGSSGGAAAAVSSGMV
                     PIAGASDGGGSIRIPASFNGLIGLKPSRGRIPVGPSSYRGWQGASSHFALTKSVRDTK
                     RLLYYLQSYQVESPFPLKKLSKESLFEFSVSKPLKIAVLMDSPLKTKVSSEAKAAIKE
                     AADFLSQKGNHLELVEQPLDGIHSMKTYCMMNSVETAAMFDDIEKSLGRSMEFSDMEL
                     MTWAMYQSGQRVLAKDYSKLLDSWDQFAATMARFHENYDLILTAATNQPAPFHGQFDL
                     DETLQKQLRHMGEFSVSEQQDLIWKMFEDSMAWTPFTHQPNLTGQPSLAIPTHLTKEG
                     LPLGVQLTAAKGREDLLLAVAELFEKEKQFKGPVYH"
     gene            complement(30796..31278)
                     /gene="greA"
                     /locus_tag="VBC52_000338"
     CDS             complement(30796..31278)
                     /gene="greA"
                     /locus_tag="VBC52_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEO6881945.1"
                     /translation="MAEKTYPMTQVEKDQLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQIQILETKIRYAEIIDSDAVAKDEVAIGKTVLVQEVGTND
                     KDTYHIVGAAGADIFSGKISNESPIAHALIGKKTGDLATIESPAGSYQVEIISVEKTN
                     "
     gene            complement(31351..33153)
                     /gene="mltG"
                     /locus_tag="VBC52_000339"
     CDS             complement(31351..33153)
                     /gene="mltG"
                     /locus_tag="VBC52_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEO6881946.1"
                     /translation="MTEFNDDQHSNHDQKSFKEQILAELEEANRLRKLREEELYQKEQ
                     EAKEAARRTAQLMADYEAQRLKDEQEARAKALETKQRLEEQEKARIEAKLLAEAAREE
                     ERRQAEQALASQEEQVINQGMEPSRELDSGSKSSEFRTTENVPDIDLKADKTDVATAV
                     PNQETEEIFLVRATDIPTEGENVKLGETSELEPVAKEPIRVEDLSKEEEGIALSAKNK
                     HNKRERRQKADNVAKRIARILISIIILVLLLTAFVGYRFVDSAIKPVDSNSNKFVQVE
                     IPIGSGNKLIGQILEKAGVIKSATVFNYYSKFKNYSNFQSGYYNLKKSMTLDQIAAEL
                     EKGGTAEPTKPALGKILITEGYTIKQIAKAIESNKIDTKTTSTPYKADDFLKLVQDET
                     FIKKMVAKYPNLLGSLPDKSKAIYQLEGYLFPATYNYYKDTTLEGLVEDMISTMNTKM
                     APYYNTIKAKNMSVNDVLTLSSLVEKEGSTDEDRRKIASVFYNRLSAGQALQSNIAIL
                     YAMGKLGDKTSLAEDAQINTSIKSPYNIYTNTGLMPGPVDSPSISAIEATIKPASTDY
                     LYFVADVKTGNVYYAKDFETHKANVEKYINSQIN"
     gene            complement(33240..33743)
                     /locus_tag="VBC52_000340"
     CDS             complement(33240..33743)
                     /locus_tag="VBC52_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6881947.1"
                     /translation="MIIRNGCLEDLQQVISIEQINFSEAEAASKKAMQERLTIMTDTF
                     LVAEINGRLAGYIEGPVIKGRYLTDDLFHKVSEFPVRVGGFIGITSLSIHPDFKGQGI
                     GTALLAAMKDLVVSQERDGISLTCHDDLISFYEMNGFKDEGESDSQHGGSLWYNMIWN
                     NPTRSIC"
     gene            complement(33829..35160)
                     /locus_tag="VBC52_000341"
     CDS             complement(33829..35160)
                     /locus_tag="VBC52_000341"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEO6881948.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHNVQGSDVDKYYFTQRGL
                     EQAGVTILPFSPNNISEDLEIIAGNAFRPDNNEELAYVIEKGYHFKRYHEFLGDFMRQ
                     FTSLGVAGAHGKTSTTGLLAHVLKNITDTSFLIGDGTGRGSANANYFVFEADEYERHF
                     MPYHPEYSIITNIDFDHPDYFTGLEDVFNAFNDYAKQVQKGLFIYGEDPKLHEITSEA
                     PIYYYGFEDSNDFIAKDITRTVNGSDFKVFYNQEEIGQFHVPAYGKHNILNATAVIAN
                     LYIMGIDMALVAEHLKTFSGVKRRFTEKIIDDTVIIDDFAHHPTEIIATLDAARQKYP
                     SKEIVAIFQPHTFTRTIALLDEFAHALSQADSVYLAQIYGSAREVDNGEVKVEDLAAK
                     IVKHSDLVTVENVSPLLNHDNAVYVFMGAGDIQLYERSFEELLANLTKNTQ"
     gene            complement(35170..35787)
                     /locus_tag="VBC52_000342"
     CDS             complement(35170..35787)
                     /locus_tag="VBC52_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881949.1"
                     /translation="MNRGSRRFPLIADDEPVMSPLVKMNLYDNEDLINNIRGFYQEKT
                     YQSMVKSNYEHEEISHPKVIENDPVPPQSFVKKATELSKSRQEAKRSVREKRQAYYAK
                     QEFKAPSKEAFQQQLKATVPKKQTQRKVTELSHLSDRLQQESYILAEIPIIFQEPDNT
                     PNPKTKKNNFDFLKRSQVYNKQDNQFHKERAKAQELNLTRFKDIN"
     gene            complement(35953..39051)
                     /locus_tag="VBC52_000343"
     CDS             complement(35953..39051)
                     /locus_tag="VBC52_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6881950.1"
                     /translation="MSRMIPGRIRNQGIELYEQGLVSLISQEGNLLKAKVGDCQIEYS
                     LVTEETKCSCDFFARKGYCQHLAALEHFLKNDPEGKAILSKVQVQQESQQETKKKTSF
                     GSVFLDSLIINEDDTIKYQLSAQGEQNPYANDIWWTLKIRRLPDDRSYVIRDIKAFLN
                     TVRKEAYYQIGKQYFETLSLIQFDETSQELIEFLWRLIPSHSSKIDLEFILPNQGRHL
                     SLTRGFFEEGVTLMNALENFSFESDFHQFNHLYFKELEGEDHLYQFKVIVHRQSIELE
                     IKEKDLKPLFANSYLFYRDTFYHLNLKQEKMVTAIRSLPIEGDLAKHIHFDLDDQDKL
                     AAHLLDFKEIGLVDAPRSFSIHDFKVNFEFDINSQNEILLQMVFDYGNDLTVHNRQEL
                     EQLTFASHFKHEEKVFKLLEKYGFAPHFSTSHPAYSAQELYDFYTYMLPQFKKMGTVS
                     LSAKLESYRLIERPQIDIEAKGSLLDISFDFSDLLENDVDQALVALFDNNPYFVNKSG
                     QLVIFDEETKKVSATLQGLRARRAKNGHIELDNIAAFQLSELFANQDNVSFSQHFYQL
                     IEDLRHPEKFKIPGLSVSASLRDYQLTGVRWLSMLDHYGFAGILADDMGLGKTLQTIS
                     FLSTKLTRDSRVLILSPSSLIYNWQDEFHKFSPDVDVAVAYGSKIRRDEIIAERHQVI
                     ITSYSSFRQDFETYSEGNYDYLILDEAQVMKNAQTKIAHSLRSFEVKNCFALSGTPIE
                     NKLLEIWSIFQIILPGLLPGKKEFLKLNPKQVARYIKPFVMRRRKEEVLPELPDLIEM
                     NYPNEMTDSQKVIYLAQLRQIQESIQHSSDADLNRRKIEILSGITRLRQICDTPRLFM
                     DYDGESGKLESLRQLLTQIKENGHRALIFSQFRGMLDIAEREMVAMGLTTYKITGSTP
                     ANERHEMTRAFNAGSKDAFLISLKAGGVGLNLTGADTVVLIDLWWNPAVEMQAISRAH
                     RLGQKENVEVYRLITRGTIEEKILEMQETKKHLVTTVLDGNETHASMSVDDIREILGV
                     SK"
     gene            complement(39206..40516)
                     /gene="der"
                     /locus_tag="VBC52_000344"
     CDS             complement(39206..40516)
                     /gene="der"
                     /locus_tag="VBC52_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEO6881951.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYT
                     TGEWLNRKFSLIDTGGIDDVDAPFMEQIKHQADIAMTEADVIVFVVSGKEGVTDADEY
                     VSRILYKTNKPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPLSSVHGIGTGDILDAIV
                     ENLPVEEENENPDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTNFV
                     DSQGQEYTMIDTAGMRKSGKVYENTEKYSVMRSMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHETGKGIIIVVNKWDTIEKDNHTVSQWEADIRDNFQFLSYAPIIFVSAETKQ
                     RLHKLPDMIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYLRFLENQIREAFVFEGTPINLIARKRK"
     gene            complement(40564..41466)
                     /gene="dnaI"
                     /locus_tag="VBC52_000345"
     CDS             complement(40564..41466)
                     /gene="dnaI"
                     /locus_tag="VBC52_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEO6881952.1"
                     /translation="MKSVGQALENQGRVPRNTNDELIQMILADAQVAEFIKTHQLSQR
                     EINISMSKFNQFLIERQKFKNKDSQYIAKGYEPILVMNEGYADVSYLETRELIEAQKK
                     QAISDRINLVNLPKSYRNIRMTDFDINNESRMKAMSQLLDFVETYPSYNHKGLYLYGD
                     MGVGKSYLMAAMARELSERKGVSTTLLHFPSFAIDVKNAISSGTVKDEIDAVKSVPIL
                     ILDDIGAEQATSWVRDEILQVILQHRMLEELPTFFTSNYSFNDLERKWANIKGSDETW
                     QAKRVMERVRYLAIEFHLEGPNRR"
     gene            complement(41463..42638)
                     /locus_tag="VBC52_000346"
     CDS             complement(41463..42638)
                     /locus_tag="VBC52_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEO6881953.1"
                     /translation="MSPIDEFTYIKQNKIVYDSNSLIQLYFPIMGSDAMALYDYFVHF
                     FDDGIRRHKFSEVLNHLQYGMPRFQDALVMLTALDLLTVYQATGTYLVKLNQAMSNEL
                     FLSNPIYRRLLEKRIGEVAVAELDMKIPKNARDISKKFTDVFSDLGQPKQEVNRSKNV
                     FDLESFKRLMMRDGLRFNNEKDDVLGIYSVSELYHLNWYDTYQLAKQTAINGMIAPQR
                     MKVQQNEGQHIKDNQSFTNNEKVILRESKNDSALVFLEKIKRSRKAVTTSGEKTLLED
                     LAKMNFLDEVINVMVLYTLNKTKSANLNKAYIMKVANDFAFQNVMTAEDAVLKIRDFS
                     DQKVRTKTETKKKQSNVPEWSNPDYKDEVSPEKEIELEQFKTDALKRLERLGKDGES"
     gene            complement(42638..43117)
                     /gene="nrdR"
                     /locus_tag="VBC52_000347"
     CDS             complement(42638..43117)
                     /gene="nrdR"
                     /locus_tag="VBC52_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEO6881954.1"
                     /translation="MRCPKCGYNKSSVVDSRQAEEGTTIRRRRECEKCGNRFTTFERL
                     EELPLLVIKKDGTREQFSRDKILNGIIQSAQKRPVSSEDIENCILRIERKIRSEYEDE
                     VSSITIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITKRVRSKKSGSV"
     gene            complement(43266..44771)
                     /locus_tag="VBC52_000348"
     CDS             complement(43266..44771)
                     /locus_tag="VBC52_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEO6881955.1"
                     /translation="MKNKKDQFIGVKQPLSKKLSQLVFILFFSLFTVFSVLVYTSATR
                     YVLHREKINVGRSLEKTRVRLSQANSSLTSDDILEILYNQVFADDIYPHKRQNGIVRT
                     GESIDSILYVNQEMTLYDVNRKPVFSTLRTGMPTIGKSMGKVIISKVADMEGFVGTKA
                     IYSQKTGQLLGYVQIFYNLGRYYSMRQNIIVFLIMMEVLGTVLALVVINSATKRIVRP
                     VKNLHDLMHQISENPSNLEIRSKVRSEDEIGELSRIFDGMLDQLEDYTRRQSQFISDV
                     SHELRTPVAVVKGHIGLLQRWGKDDPEILEESLAAAYHEADRMSLMINDMLNMIRVQG
                     SLELHQDEMTDLSSSISVVIENFRILREDFQFIFENNISDIVWGKIYKIHFEQALMIL
                     IDNAIKYSPSYKEVSVVLSVDNDFATVVVKDKGEGISDEDIEFIFDRFYRTDKSRNRE
                     STQAGLGIGLSVFKQIMDAYHLKVDIKSELNQGTEFIVRIPIKKFEETKEI"
     gene            complement(44761..45450)
                     /locus_tag="VBC52_000349"
     CDS             complement(44761..45450)
                     /locus_tag="VBC52_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6881956.1"
                     /translation="MGKKILIIEDEKNLARFVSLELLHEGYDVVVETNGREGLDTALE
                     KDFDLILLDLMLPEMDGFEITRRLQAEKTTYIMMMTARDSVMDIVAGLDRGADDYIVK
                     PFAIEELLARVRAIFRRQEIETKTKEKGDSGSFRDLSLNTHNRSAMRGDEEISLTKRE
                     FDLLNVLMTNMNRVMTREELLEHVWKYDVAAETNVVDVYIRYLRGKIDIPGRESYIQT
                     VRGMGYVIREK"
     gene            complement(45685..46218)
                     /locus_tag="VBC52_000350"
     CDS             complement(45685..46218)
                     /locus_tag="VBC52_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEO6881957.1"
                     /translation="MLLTEIKKSPEGLYFDKKIDIKESLMERHSEIMDISDIQVSGHV
                     VYEDGLYLLDYNMAYDITLPSSRSMKPVVLSEKQTINEVFIEAENVSTKKELVDQDLV
                     LILEEDDINLEESVIDNILLNIPLRVLAADEVGVEADLSGKNWSLMTEKQYEEKQAKE
                     KEKSNPFAALEGMFDSD"
     gene            complement(46323..47213)
                     /gene="htpX"
                     /locus_tag="VBC52_000351"
     CDS             complement(46323..47213)
                     /gene="htpX"
                     /locus_tag="VBC52_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEO6881958.1"
                     /translation="MLYQQIASNKRKTVVLLIVFFCLLAAIGAAVGYLVLGSYQFGLV
                     LALIIGVIYAVSMIFQSTNVVMSMNNAREVTEDEAPNYFHIVEDMAMIAQIPMPRVFI
                     VEDDSLNAFATGSKPENAAVAATTGLLAVMNREELEGVIGHEVSHIRNYDIRISTIAV
                     ALASAVTLISSIGSRMLFYGGGRRRDDDREDGGNILVLIFSILSLILAPLAASLVQLA
                     ISRQREYLADASSVELTRNPQGMISALEKLDRSEPMGHPVDDASAALYINDPTKKEGL
                     KSLFYTHPPIADRIERLRHM"
     gene            complement(47234..47788)
                     /locus_tag="VBC52_000352"
     CDS             complement(47234..47788)
                     /locus_tag="VBC52_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEO6881959.1"
                     /translation="MGTMILIAIIALFVIWLIVAYNSLVRSRMHTKESWSQIDVQLKR
                     RNDLIPNLIETVKGYAAYEGKTLEKIAELRAQVAKANTPAEAMTASNELTRQLSSILA
                     VAENYPDLKANNSFVKLQEELTNTENKISYSRQLYNTTTSNYNVKLETFPSNIVGKLF
                     GFKPSQFLETPEEEKEVPKVSFDF"
     gene            47916..48629
                     /gene="rsmG"
                     /locus_tag="VBC52_000353"
     CDS             47916..48629
                     /gene="rsmG"
                     /locus_tag="VBC52_000353"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEO6881960.1"
                     /translation="MTPQAFYQVLIEHGITLTDKQKKQFETYFRLLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGYIDNSPLSILDIGAGAGFPSIPMKILYPEIDITIIDS
                     LNKRINFLNILANELELSGVHFFHGRAEDFGQDKVFRAKFDIVTARAVARMQVLAELT
                     IPFLKVNGRLIALKAAAAEEELISAEKALKTLFSQVTVNKNYKLPNGDDRNITIVSKK
                     KETPNKYPRKAGTPNKKPL"
     gene            48644..50023
                     /locus_tag="VBC52_000354"
     CDS             48644..50023
                     /locus_tag="VBC52_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO6881961.1"
                     /translation="MKHFFDYKTMSVARKLSISFIAVILLGSILLSLPIFQYANAPKT
                     HYIDHLFTTVSMVCVTGLSVFPISKVYNGWGQIVAILLMQTGGLGLVTLMSLSYYTLR
                     RKMSLNDQTLLQSAITYNSSTDLKKYLYMIFKVTLTLEVLAASILAIDFIPRFGLGHG
                     IFNSIFLAVSAFCNAGFDNLGATSLAQFKLNPLVNIIVCFLIISGGLGFAVWKDLIEA
                     TIQTSHKGPKLIKTFPKRLSNHSKLVLKTTTIILLTGTLLSWLLEFGNFRTIANLSLP
                     KQLMVSFFQTVTMRTAGFSTIDYTQTDFATNLVYIIQMLIGGAPGGTAGGFKVTVIAI
                     LLLLFKAELSGQSQVTFHYRTIPSSIIKQTLSILTFFFIILISGYLLLLELNPHIDPF
                     SLFFEASSALATVGVTMNTTNQLTLGGRIVIMFLMFIGRVGPITVLLSILQKKEKEIH
                     YAETEIILG"
     gene            50036..50707
                     /locus_tag="VBC52_000355"
     CDS             50036..50707
                     /locus_tag="VBC52_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEO6881962.1"
                     /translation="MKTKIIGVLGLGIFGQTLAQELSNFEQDVIAIDSNPENVQAVAE
                     VVTKAAIGDITDLAFLKHIGISDCDTVIIATGNSLESSVLAVMHCKKLGVPQVIAKAR
                     NLVYEEVLYEIGADLVISPERESGQNVAANLMRNKITDVFQIESDISVIEFKIPKSWV
                     GKTVEQLNIRHKFDLNLIGIRKAKNKPVDTEVPINSPLEEGIILVAIANSDAFQRYDY
                     LGYFN"
     gene            complement(50742..51572)
                     /locus_tag="VBC52_000356"
     CDS             complement(50742..51572)
                     /locus_tag="VBC52_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO6881963.1"
                     /translation="MAKQLIGYQEGYGFLYRLSGASKLLFFILVSVACMTTYDTRFLI
                     VVSLGSLVLFRLAKIKWQQVSFVMTLVVVFAVLNIIMVYLFAPHYGDKIYGSSSLLLK
                     GIGPYDVTSQELFYLFNLILKYFCTVPLALLFLMTTNPSQFASSLNQLGLSYKIAYAV
                     SLTLRYIPDVQEEFYTIRRAQEARGIELSKKSNLVARIKGNLQIVTPLIFSSLERIDT
                     VATAMELRRFGKNKRRTWYSKQSLEKSDIVLIILALASLFVSLYLIHLNHGRFYNPWQ
                     "
     gene            complement(51565..53241)
                     /locus_tag="VBC52_000357"
     CDS             complement(51565..53241)
                     /locus_tag="VBC52_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6881964.1"
                     /translation="MKDFIEWKDFTFQYDVQSEPTLKGINLSIPKGEKVLILGPSGSG
                     KSTLGHCLNGIIPNTHKGQYSGIFTINHKNAFDLSIYDKSHLVSTVLQDPDGQFIGLT
                     VAEDIAFALENDVVAQEEMASIVEMWAKRLEIAPLLSKRPQDLSGGQKQRVSLAGVLV
                     DDSPILLFDEPLANLDPQSGQDIMALVDRIHQEQDATTIIIEHRLEDVLYERVDRVVL
                     FSDGQIIYNGEPDQLLKTNFLSEYGIREPLYISALKNLGYDFEKQNTMTSIDDFDFSE
                     LLIPKMRALDLDKHTDKLLSVQHLSVSYDLENNTLDDVSFDLYKGQRLAIVGKNGAGK
                     STLAKALCQFIPNNATLIYNNEDVSQDSIKERAERIGYVLQNPNQMISQAMVFDEVAL
                     GLRLRGFSDNDIESRVYDILKVCGLYQFRNWPISALSFGQKKRVTIASILILNPEVII
                     LDEPTAGQDMKHYTEMMSFLDKLSCDGHTIVMITHDMQLMLEYTDRAIVIDNGLIVAD
                     DHPINILSNTELLKKTHLKKTSLFALADRLGISPQKLTQWYIDNQGGKNG"
     gene            complement(53258..53806)
                     /locus_tag="VBC52_000358"
     CDS             complement(53258..53806)
                     /locus_tag="VBC52_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEO6881965.1"
                     /translation="MNTNTIKKVVATGIGAALFIIIGMLVNIPTPIPNTNIQLQYAVL
                     ALFAVIYGPGVGFFTGFIGHALKDSIQYGSPWWTWVLVSGLLGLMIGFFAKKLAIQLS
                     GMTKKDLLLFNVVQVIANLIGWSVVAPYGDIFFYSEPASKVFAQGFLSSLVNSITIGV
                     GGTLLLLAYAKSRPQKGSLSKD"
     gene            complement(53975..55183)
                     /gene="sstT"
                     /locus_tag="VBC52_000359"
     CDS             complement(53975..55183)
                     /gene="sstT"
                     /locus_tag="VBC52_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEO6881966.1"
                     /translation="MKRFLKAWRKTSLIKKITIGVVIGLFLGILVPKASAIGLLGQLF
                     VGGLKAIAPLLVFTLVISALSQHREGGKTNMSTIIGLYITATFAAALIAVVVNYIFPL
                     TLILKTPAKTDLLPPKGISEVFQSLLLKIVDNPIHAITEANYMSILFWAVIFGLAMRS
                     SNQRTKDLMQTFADATSQVVKWIINLAPIGIMGLVFTSISENGIGILGDYGLLILVLV
                     GTMLFVALVVNPIIAFVMMRKNPYPLVLRCLKDSGITAFFTRSSAANIPVNMRLCEDL
                     GLDKDTYSVSIPLGAAINMAGAAITINILTLAAVNTLGITVDFPTAFLLSVVAAVSAC
                     GASGVTGGSLLLIPVACSLFGISNDVAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     VAEKSVFGKK"
     gene            55359..56726
                     /gene="brnQ"
                     /locus_tag="VBC52_000360"
     CDS             55359..56726
                     /gene="brnQ"
                     /locus_tag="VBC52_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO6881967.1"
                     /translation="MKLKNTYIIIGLMLFALFFGAGNLIYPAYLGIYSGSNWAWAIIG
                     FCLTGVTLPLLGVAAVAYSGATDVESFTKPVSKTYAIFFAVALYLSIGPFFAIPRTGA
                     TSFSVGIQPILGDSSLVKIIYGLIFFGISYFLAIRPSQIVDRIGKYLTPFLLTVISIL
                     IIASLIHPAAIVGTPHNASTNVSDAFKDLPFLAGLIQGYGTMDALASIAFAIIVIQAS
                     KQYGAITKKEITSMALKSGAIATFLLAFIYIFVGRIGATSQSLFKFANGSFLLHNTPI
                     DGGHVLSQSANFYLGIVGQAILGTAIFLACLTTATGLITACAEYFHKLLPKISHITWA
                     TIFTLIAITFYFGGLSEIIRWSLPVLYLLYPLTIVLIFLVFFDQKFESSRIVYQTSIA
                     ATAVAALYDALSKLGEMTGLFTIPSALTTFFTKVVPLGEYSMGWISFAICGVLVGLIL
                     KKVKA"
     gene            56840..57895
                     /locus_tag="VBC52_000361"
     CDS             56840..57895
                     /locus_tag="VBC52_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO6881968.1"
                     /translation="MRAATYLSANELSLTDKAKPQVIKPTDAVVRLVKTTICGTDLHI
                     LGGDVPACKPGTTLGHEGIGIVEEVGSAVNHFKVGDKVIISCVTACNTCYYCKHQLPS
                     HCEDGGWILGHLIEGTQAEYVHIPHADGSLYHAPEGVCDDALVMLSDILPTSYEIGVL
                     PSHIKPGDTVCIVGAGPIGLSALLTAQFYSPAKIIMVDLSQKRLEASKKFGATHTILS
                     TSTQEVKEEIDKITKGRGVDVVLECVGYPATFDICQNVVSIGGHIANVGVHGKPVEFN
                     LQDLWIKNITLNTGLVNANTTEMLLEVLETGKIDATQLVTHHFKLSEIEEAYKVFKAA
                     EENNTLKVIIENDITPA"
     gene            complement(57944..58636)
                     /locus_tag="VBC52_000362"
     CDS             complement(57944..58636)
                     /locus_tag="VBC52_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000574038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6881969.1"
                     /translation="MIEWIQTHLPNVYQMGWEGAYGWQTAIVQTLYMTFWSFLIGGLM
                     GLLGGLFLVLTSPRGVIANKLVFGVLDKVVSVFRALPFIILLALIAPVTRVIVGTTLG
                     SPAALVPLSLAVFPFFARQVQVVLAELDGGVIEAAQASGGTLWDIIVVYLREGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGSVAITKGYNYSRDDITLVATILILLLIFFIQF
                     LGDFLTRRLSHK"
     gene            complement(58633..59703)
                     /locus_tag="VBC52_000363"
     CDS             complement(58633..59703)
                     /locus_tag="VBC52_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEO6881970.1"
                     /translation="MSKEPIIKLDNIDVTFHQKKREINAVKDVTIHINQGDIYGIVGY
                     SGAGKSTLVRVINLLQEPSAGKITIDDQVIYDNKVTLTSTQLREQRREIGMIFQHFNL
                     MSQLTAEQNVVFALKHSGLSKEAKAAKVAKLLELVGLSDRAQNYPSQLSGGQKQRVAI
                     ARALANDPKILISDESTSALDPKTTKQILALLQDLNKKLGLTIVLITHEMQIVKDIAN
                     RVAVMQNGKLIEEGSVLDIFSHPRESLTQDFIKIATGIDEAMLKIEQQEVVKNLPVGS
                     KLVQLKYAGHSTDEPLLNQIYKEFEVTANILYGNIEILDGIPVGEMVVILSGDEEKLR
                     QACQAITDSQVQLTLLKEGGKA"
     gene            complement(59696..>60566)
                     /locus_tag="VBC52_000364"
     CDS             complement(59696..>60566)
                     /locus_tag="VBC52_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEO6881971.1"
                     /translation="EKYQEQLQGADLIVWEDGPKNPKGQLEIAGGNKGIVTFDLSVSS
                     ADVDIHSSFGGVVDSSTWYLIQALNTLRDNKGHILVEGIYDKVIPPTKRELELVEKYS
                     YRSAKALEGAYQLVLPSLADSHKTFLRKLYFEPSIAIEGITSGYQGEGVKTILPAYAK
                     CKAEVRLVPGLTPKGVLDSIQNHLKENGFKDIELTYTLGEMSYRSDMSAPSILKVVDL
                     AEQFYPEGISLLPTSPGTGPMYLVHQALRAPIAAIGIGHANSRDHGVDENVSIADYYT
                     HIELVEALIESYE"
BASE COUNT        19269 a        12023 c         9356 g        19918 t
ORIGIN      
        1 acttatttat tccaataggt aaatcggaga ttataaaagg ttgccaaagt tttctttggc
       61 aaccttttta tattattact tataaatcat ggaaactagc gtagagttct tgtctattga
      121 gattaagttc ttttgctact ttttttatcg cttgattagt tttatcgcca ttagcgatat
      181 attcttttac taatactagt ggatcttgtt ggctactgtc tttcactcgc tcagtatctc
      241 tcttaccatc aacaataatt aagcattcac ctttgagagg gaccttttca atatgcccta
      301 aaagttgact aatggttcct ctttgatact cttcatagag tttcgtcaat tcgcgtacta
      361 aaacaacttg gcgatctcca taaatctctt tcatgtgttt tagcgtatca gagactcgaa
      421 acggtgactc ataaaagatt tgtgtttcag ggtaatcttt ctttgtttca aaaaaagtta
      481 tttgttgacc ttgcttacgc ggtaagaagc cataaaaaat atgaggttgt gaagctaaac
      541 ctgaagcgat gagagcagta ataccagcgc tagctcctgg tatagatacg accgggatat
      601 ccccttcaat agcagccttg acaaggtcat gtcctgggtc agaaatagag ggcattcctg
      661 catcagatac ttgggctaaa gatctccctt cttttaacaa atcaattaac ccagagattt
      721 tatcataagc attgtgttcg tgaaaactaa tttgtttagt agtaatatca aagtgcttga
      781 gtaaaagtcc cgtatttcgt gtatcctctg cacaaataaa atcaacttct cttaaaatcc
      841 taatggcacg aaaagtcata tcatctagat taccaattgg agttgggact agatagagtg
      901 tcccgtaatg tgtatttgat ttaaaacttt tttgaacttg catttccatc aatctctata
      961 taataattca atacaaaaag cacattcttc gtcattttca cgacgttgac catagaaagt
     1021 attacaaatg tgaaagccat cattgtaaat tccttcgagt agttctcttc cttgtttaga
     1081 atttttaact gcagtttctg tttctgcttc aataacgctc agacgctcac gaagtttacc
     1141 attttcaata cgtagaacag tattctcctc caataatttt tggatttgtt ttttcatagt
     1201 ctcaatttca gacaacccaa ctaataaatt ttgtgaaaaa tcatcaaagg catcaaacaa
     1261 gtctttttta tccattcact cttcctctct gcgcgtatta tttgtctaag aaagcaccat
     1321 atactctaaa ctattttgaa aactaacatt gctcttccac atttgctgag ctctataagt
     1381 attttctaaa tcataaagtt ggccttcttt agccagcatt attgtcagcc attgaaaaac
     1441 aaatgcctga tcatttttat ctacaacttg cgatgtcaag cgagatactt ctaaataagc
     1501 agtttgccta tcttttgcca aggtcgtcac aaactgttga cttaaattca agtaatctaa
     1561 taactttttg ttagccaaca atatttctaa atgtttagga tcgtctgcta cttgagctaa
     1621 taagctagct tgtgatttta gcaatcctgc ttgttcagct tgatgaacca acatgtccaa
     1681 ttgttttgga aaacgaaaaa tttgcgttct actcttaatt gttggcaaaa cattgttttc
     1741 atcattagtc aataagatga cataagatga actttgaggt tcttcaataa atttcaaaag
     1801 gctgttggct gcattgacat gcattttttc gcaatctttg atgataaaga cctgtgattt
     1861 cccttcaaaa cctgatcttg aaaaatcttt agtcagctct tttatagtct ctgtcttaat
     1921 aagttgcccc tgtggttcta tgattttaac atctgaaaat tctccattag cgattaaacg
     1981 acaagctcta cacttttggc atggtaaacc actctggcgc ttttcacaga actgactttg
     2041 tgctaaataa agtgccatat ctagacttgc aaaatttccc gaaaaaagat aggcatgact
     2101 catcctatct gactgtaaaa tagtattaaa cttttccagc aatttgggtt gtgttcgctt
     2161 taaatccatg acctttgtac ttcctgattc attggttgat acgatctaaa ataatagaga
     2221 atgtctcagc tattacttca tctagctgtt gggaggcatc aatcgtcaca atgcggtttg
     2281 gctctaaatc tgccaattca aggtaacctt gacggacgcg ttgatgcata tctaattgct
     2341 cgagatctag acgatttatc tccctttgaa cacttttttg aatacgctct aaacctactt
     2401 cagaaggaac atcaaaatat aaagttaaat caggtttatg actatcagtc gcatagtcat
     2461 taagccattt tatatgtgac ttatctaatc ctcttcctga tccctgatag gcaacagaac
     2521 tatcaataaa acgatccatt aggactattt tcccttcttc tagagctggg agtacctttt
     2581 ccactagatg ttggcgtcga gcagccatgt aaagcaatag ctctgttttt ttatccattt
     2641 gcgtgtgttt aacatctaaa ataatatgac gaatttcttc tgaaatagtt acgccaccag
     2701 gctctctggt ggtgagaata tcttgtgata atttttctct gagcaagggt acaacagctt
     2761 ctaaaacggt tgttttaccc gcaccatctg ggccctcaaa agaaatcatt agtccttttt
     2821 tcatggtaca tcctttattt ataattacta ctattctatc aaaatttatc aaaaaaagac
     2881 acccacaact tgattgtgca tgtctgatat ttttaagaaa gtggtttttt cacggtcttt
     2941 tctaaagttt ccacaataac atcatcaaat actagttctt gttttaaaac atccttgtca
     3001 gcgtatcctt ctagttgtat ctcgaccgct ttcttacctg cttttcgttc aataatgact
     3061 aaacgtttaa cacttaaatt tgcactagaa agacgattta ggacttttga tagtacccct
     3121 atcccctcat cagctaatat aaccaagcga taaccttctt gaccataccc cgcaatttca
     3181 agaaaggctt taaaaacatc cctatccgtg acaatcccat aaagttgtcc attatcaact
     3241 actggtaaaa caccgatctt tcgagacatc atcaaataaa tagcgtcctc aaggctagca
     3301 tactgtgaaa cagtcacaat atctttaatc ataatatctc gaatttttgt cttattaaga
     3361 agatagttca tctcatatat agaaagactg gttgctttag aaggttgtgc ttctgccatt
     3421 gtcccttctg tcactaaccc cactagctga tcattttcaa caactggcaa acgccttaag
     3481 tggtgttctc tcaataaatc agcagcttct gcaactgtag tgtctggaga gacataaact
     3541 aatttttttg tcataaaatc tttcactggc atacctgact cctctaaata ttatccacct
     3601 aagtaagctt tacgaacttg atctgaaact agtaattcct tacctgtccc tgataaaaca
     3661 accttcccag tctctagaac atatgcttta tcagcaattg tcagagcttt atttgcattt
     3721 tgttcaacta aaagaactgt agtcccttgt tttttgatgt cttcaatgat attaaaaatt
     3781 tcttgaataa aaattggagc taggcccatt gacggttcat ctaaaagtaa taatttagga
     3841 cgactcatca atgcacgccc catagcgagc atttgctgct caccacctga taaggttgct
     3901 gcatcttggt ttttacgttc ttctaagcga ggaaaacgat caaacacttt ttttaacatt
     3961 ttttgatttt gttcacgatc cttttgaaga aaggctccca tttctaaatt ttccataaca
     4021 gacaaacttg agaaaacgtg gcgcccttct ggaacctgag ctaaaccatt gccaacaatt
     4081 tttctagctg ctaacttatg tataggtttc cccataaaag aaatagaagc ctgacttggt
     4141 cttactaaac cagaaattgt cctcaaaatt gatgtctttc ctgcaccatt ggctccaatt
     4201 aaggttataa cctcaccttg gttaacttca aacgagacat cattcaccgc ttgaataaca
     4261 ccataatgaa tagatagatt ttctactttc aacatggtca ttataactca ccccccaagt
     4321 atgcttctat aacacgttta ttatttttaa tttcttcagg tgtcccgtga gcaatcagac
     4381 gaccatattc caatacataa attctctctg ttacttgcat aaccaagttc atatcatgtt
     4441 caatcagcat aatagtaata tcaaaatcac tctttatttg actaattaac tgcgtcaatt
     4501 ctgcagtttc ttgaggattc attcctgccg cgggctcatc caaaaacaat atttttggtt
     4561 ctgttactaa agcacgaaca atttctaaac gccgttgttt tccataaggg aggttcttgg
     4621 caagagcatc ttgatatgct ttcaaaccaa aaatctctaa tagctctagt gcctttttcc
     4681 ttaaggcttt ttcactatga tagtatttgg gtaatcgtaa aaaactagca ataggatgtg
     4741 ataagtgatg attactgagt cctactagta cattgtctaa aacagtcata tttttaaaaa
     4801 gtcggatatt ttgaaaagtt ctacctaaac ctaaagaagc tattttagct ggtttcctcc
     4861 catttaatat cttaccatca agtgatattg tccctttact aggaaggtat actcctgtta
     4921 agagattaaa aagcgttgtc ttacccgcac cgttggggcc gattaagcca attaattcac
     4981 ctttatgtaa tttcatcgaa acatcaccta cagcagttag gccaccgaaa tgtttactta
     5041 aattttttac ttcaagaagt gccatctaaa tgcctccctt actcgtctta cgaaatagat
     5101 gagatagagt taactctttc gttcctaata aaccgctagg tctaaaaatc ataattaaaa
     5161 tcagggctaa agcatagata atcattcgaa cagaagcgta gtcttgtagt aacatattca
     5221 ataatcctaa taaaattgct gctaaaactg tccctgtcat tgaacctaaa ccacctaaaa
     5281 caacaataat taaaacattg acagacatca taaatgaaaa gtcttttggc ataacagttc
     5341 caacgtagcc agcctgtaaa ctaccagcga tagaagctaa aatagctcca aaaataaaaa
     5401 caattacttt cacttttgtt gtatccacac ccatagactc tgctgcaatt tcatcttctc
     5461 gtatagcaat tgtattacgc cctaagggac tacgcaaaaa attcattgca aggataagac
     5521 tcacaactgc caccccataa actaaagacc aggttgtaaa aggcggaatc cccatgatac
     5581 ccgcagcacc attcgtaata tcacctccat taaccaacaa aatacggata atctcagcaa
     5641 cacctaaagt agctatagct aaataatcac cttttagtct caaagtagga attcctacac
     5701 ctaaggcaat acctcctgca acaagagttc caacaagtat agctatcaaa aaacctacat
     5761 aagttggcat tttaacgccg ataatagcaa ctgcataagc gccaatagcc atgaaacctg
     5821 catgtcctag tgaaaactgt cccgaacaac ctacaattag gttcaaacca actgctaaaa
     5881 taacattaat cccaattgtt tcaataattt gaatatgata gaggttaaat atacccatag
     5941 atattagcac gctaatcaag agataaccta acactactat agctaaccaa gataaaattg
     6001 atttcatatt ttctttcatg cctagacctt ctccttaata ttttttccaa gaattcctgc
     6061 tggacggatc aagaaaatga gaattaaaat agcataaacg ataccatctc gaaaatctga
     6121 cacaccaagt gcagtcgcta aagtttctaa aatgccaata acaaaaccac cgatagccgc
     6181 tcccggaatg ataccaatac cacctaagac agctgcaaca aatgctttca accctggagt
     6241 tacccccata agaggttgaa cagaattata ataaagccca atcagtactc cacctgctcc
     6301 agctaaagca gaccccagcg caaatgtaaa actaatggta cggttaacat taattcccat
     6361 caattgtgcg gcatcgctat ccactgataa agctctcata gctttcccca ttttagtttt
     6421 ttgaacgatg aactgtaaag taagcattag taatagcgca atacctaaaa tgatgagctg
     6481 aacattggtt atagttatcg gtcctaaatt atatttaaca gtatgaatgg cttgcggaaa
     6541 tgccctagta tccgctccaa caaggtatac catcccgtac tccaaaagaa aagaaacacc
     6601 aatagctgta attaacgcgg caattctagt tgattttctc agtggacgat aagctaaata
     6661 ttcaattaca gcacctagga aagcagaccc cagcattgca attaataaag ctaggaaaaa
     6721 attaagatga agatgattaa tcaaataata gcccatgaaa gcgcccatca tatagatgtc
     6781 cccatgagca aaattaatca gcttaataat gccatatacc atagtatatc ctaaggcaag
     6841 taaagcatat atggatccta agataagtcc attaatgagc tgttgaagca tattataacc
     6901 atactttcta ttatatatta ttgtcgaatt attctgaaaa attacttttt aaaaaagggg
     6961 agctgaaaat taaactccca tttttctaga gaatacttat tttgctgaaa taatgttaac
     7021 gctacttgtt tttccatcgt ctaatttgac aacgtaagct gatttaacaa cattatgatt
     7081 tttatcaata gacattttcc ctgtcacacc tttaaaatct ttcagcttag ctaaagcttt
     7141 tttaagatca attgaagttt tagctccttt agcagattta gctgccatat acacggcatc
     7201 gtatgataaa gccgagaaca tggatggttc ttcaccatat gccttaacgt aatgatcatg
     7261 gaaagcttta gctttggttg atccttgtgt agtgaaacct gtcaaataat aaacgtttga
     7321 agctcccaca ggtgttgccg attgcacaaa tttcggacta tcaaaaccat caggcccaag
     7381 aaccggttta gagataccta aatcacgcgc ttgcttaact attaatcctg tctcggtata
     7441 gtaacctggc atcacaatag catcatattc tttccctttc aacttagtca atgacgcttg
     7501 gaaatcagta tcaccagcgg agaatgtcat gctatcaaca atttttccac tataactttc
     7561 cttaaatgat tttgctaccc cctttgagta atctgatgaa ttatcataaa atagaacaac
     7621 ttttttagct ttaaggttgt ctgtagcata ctgagacaat agctttcctt gataattatc
     7681 gacaaaagta gctcggaaga aatactgatt taaggttttt ccatcagaac catagactaa
     7741 attgtcttgt gtcgcagcag gtgcaatcat tggaactgct gctgcattta cttttggagt
     7801 tgaagatgca gctgcacccg atgttgctgg tccgataatg acattagctc ctttggtaac
     7861 caagtttgtg gcaaccgttg ctgcctctgc attttctgat ttattatcct gtgataggac
     7921 ttttaacttc ttgccgccaa cccctccagc agcattaatt tcttttactg ctaagtcagc
     7981 tccattcttc attgaatttc catacgaaga aacgtttcct gataattccc agttataccc
     8041 taccttaata tctttcccta tttttgttcc tgtcgaagat gctcctcctc cgacatttcc
     8101 acatgctgct aaaagaactg tactagctaa aacaagagct cctaaactaa gtctcttttc
     8161 catatttttc tccttttttg aaaatggtaa ctaaaataat acagaattat cagaatattg
     8221 tcaatagttt ttaaattatt tttttgaaaa ttatagaaac taaataaaaa agaaggacat
     8281 ccgaactcct tctttctaat aactactata agtatcttag cgatataagt ttccaacaaa
     8341 atcctgatca atatcatcaa ttgctgataa ccgaacatcc ttaacaaatt ttaaatgttt
     8401 tatctcttta ctaacgctaa caatatcctc tttattaaca taaatgacta gatatcttaa
     8461 tctacgagaa tgataatgaa catctccgta tttatataat ttacgagcgt cacgattata
     8521 gtaaagataa acgctcattc ctaaacgctc ttgtttattc aataattttt tcctttctgc
     8581 gtcatatctg taattactaa gattataaca taattccaac aaataaaaaa agagttgata
     8641 aactcaactc ttttaaatct attattgtag attattattt tccataatag catcaataaa
     8701 gccataatca agtgtttctt gagcactcat ccaacgatca cgctctgcat catcatggac
     8761 tttttcaata gattgaccag aattatcagc taagattttt tctaaagtat gacgcgtttt
     8821 taaaagatgc tcagcagcga tagccatatc agattgctgt gtgcctccgc ctgttccacc
     8881 cattggttgg tggatcatat attctgcatt cggtaacata aaacgttttc ctttagcacc
     8941 actcgaagca ataatggttc ccatcgaagc agccatcccc ataacaatcg tctgtacgtc
     9001 cgatttaata aagttcatgg tatccacaat agcaagtcca gccgatactg aaccacctgg
     9061 tgtattgaca taaaggtaaa tatcctttgt attatcttgt gcatcgagaa acaataactg
     9121 tgcaatgata ctattggcca tattatcctc aacttggccg gtcaacataa taatacgatc
     9181 ttttaaaaga cgtgagtaaa tatcataaga gcgttcacca cgacttgttt gttcaataac
     9241 tactggaatc atatctttct ccatttcttt ttatcactgt cactattata atcatttggt
     9301 caacaaaggt caaataataa cttttttaag aaaaggcttt ctgagttctt cagaaaagcc
     9361 ttattcatct caaatcttat tttgtaccaa ataagcgatc accagcatct ccgagtccag
     9421 gaacaatgta gccatgttca ttgagttttt catccaaagc agcggtataa atatctacat
     9481 ctggatgtgc ttcttgaagt gctgccactc cttctggagc tgctactaaa caaacgaatt
     9541 tgatactagc tgcgccacgt ttttttagag aatctactgc taaaatagca gacccacctg
     9601 tagcaagcat tggatcaact acaaaaatct gacgttgatc aatatcttca ggtaacttaa
     9661 ctaaatattc gactggttgg aaagtctctt catcacgata cataccgata tgaccaactt
     9721 tagcagctgg cacaaggctt aagaaaccat caaccatacc aatacctgcg cgtaaaattg
     9781 gaacaattgc taactttttc ccagctaatt gtttttgtac agttgtcgct actggtgttt
     9841 ggatttcaac atcttctaaa ggcaaatcac gagacacttc atagcccatt aacatcgcaa
     9901 tctcatcaac taactcacga aaatctttag tagatgttgt agtgcgacgt aagattgaca
     9961 gtttatgttg aataagtgga tgtgaaatga cttgaaattt tcccattata atgaccgctt
    10021 tctatttaat tttgagcatt aaaaacattc taacgcttgt ttacattcaa agtattataa
    10081 cataaaaacc ctttccatgc gacttttttt tatagtctag tcattttttc taaagtttgt
    10141 cactaatcgc aaaactgcct cctcaataac agacctagga gctgcgacat tgattctagc
    10201 atgttttttc ccttcttttc caaaagttaa accatcattt aatattaatt tggcatcata
    10261 tcgaagtttt tcttgaattt ctaaatgcgt taaattataa gcagaaaaat ctaaccaaac
    10321 aagataagtt ccctctggtt tcatcacttt gatatgagtt ttttgcgtta attgctcata
    10381 aaggtattct attgatcctt ctaactccat ttttagggcc tttaaccatt gtttcccttt
    10441 tgtaaaggca acttctgtag ctaataaccc caatgaggaa atttcttgtt gattgttagc
    10501 tatttgcctc ttcttaaaat cagagcgtaa cttttcattt tcaattattg caaaactatt
    10561 tttagtccct gcaatattaa acgttttagt tgcactagat aaaataacac tcaattcttt
    10621 aaaagtagaa tcaacagtat taaagctatg gtgtacatta tcaaataata ccaaatcttg
    10681 atgtatttcg tcagaaacta ggataacatt ataccgttta cagatatctc ctattttttg
    10741 aatttctcct tttgtccaga cacgcccacc gggattatgt ggactacaaa aaatataaag
    10801 ctttacatta ttttcaatga tatctttttc taattgttta acgtcaatct caaaatactg
    10861 attattatta agtaaactat ttgaaactaa atgacgatta ttgtatttta tagttctagc
    10921 aaagggcgga tagactgggg tattaattag aacagcatct ccctcctttg tgaaggcttg
    10981 tatggcaact gaaatcgctg gtataacccc atcaattaat aataatgatt ttttatcaaa
    11041 ttgataacca tgttcttgca cttcccaatt agagatagct tggtataaac tgtctttagg
    11101 actatcatag ccaaaaacca tttggtgtga aaaatcttca atagcctccg acatttctgg
    11161 aaagatagga aaatccatat ccgcaatcca aaggggaagg atttcttgat ctttttgaac
    11221 cgccttccat tttatggcat tacttgaaaa acgttctggt aaacttgtga aatcatatgt
    11281 aatcataatt actcctgact attctacagc taaaaatatc ccttgtcgcc ttggtgacta
    11341 ttttaccatt tgtttttgat actatatact aattaacatt ctaaagataa aagtgaccat
    11401 tttaaacgat cactttcttt attattttaa tttatcattt tcataagtat ggcttaattc
    11461 taaattagga aaactttgct gcctaagggc ttcataaaca atcatacaaa cagtgtttga
    11521 aacattcaag ctacgcacat gctcatcatt catgggaatt cgtaaggcct tttcggcatg
    11581 ctcgcgcata aaagtttctg gcaatccttt atcttcacga ccaaacataa aataatggtc
    11641 ttgatcatca ttataatttt catctgagta aaccttgtca gcaaatttgc taattaggtg
    11701 aactttccca cgacatgaca acataaattc ttccaaacta tcatagaaac taacatctag
    11761 cttatcccag taatccagtc ctgctcgttt cattttttta tcatcaatag gaaatcccat
    11821 cggtcgtatg atgtgcaaag gagcatttgt tgccgcacaa gttctagcaa tattaccagt
    11881 atttgctgga atctgtggtt caaataaaac aatatgattt cgtccactat cggaacgatg
    11941 atttttctgc gttaatgttt caatattcat ctcatcatca ctttctagaa ctcattctga
    12001 ctcgcaaaaa aaccacactg ctcggagtca atctcagcaa acaatgtggt ttatggtggt
    12061 ttgttagctt aaatcataac aggtttgatt taaactaaca aatcgttgac actattatag
    12121 cacttcagtc tacaaagtca acctattcat gagtttttgg gttatttttt tgatagatat
    12181 aatgacaaat caaacagaat aataagcaaa aacattggaa aattaaaacc gtaaagaaat
    12241 tacttaacaa actcccgata aagaggatca aaccgcctaa aattgctaga atcggtgaaa
    12301 ctaagccggt aaatttattt ttaatatctt ttttgagata aaggctgagt actaaaacat
    12361 aaagaatgat taaactggta ttattaaaca ccacagcaat ttcactaata tcactacccg
    12421 gcaataaatt aaattttgta accataaagt gaacaaaaag ccaaaaaata gctacagccg
    12481 tcaccgtcaa actagctggc aatgacattt gatacttcaa atttatgtta gccatcctct
    12541 cacttttgat ccatccgcgt tccgcaaaag cttgaggtag acgcatcgtc ccaagtaaaa
    12601 gtccattggc aacacctaat acagataaaa taacaatgaa agataataaa cgcccaacac
    12661 taggaccgaa aataatgtta gctgcataat taatagcatc attaccggtt gtcataataa
    12721 agctagcacc taaaatttgt gtcaaaccat agaaaaatgc caaatatgat aataagataa
    12781 gcgctggacc aatgacaaag gcaagtggta aattcttctt gggattcttg acttctggtg
    12841 caatactaac aaaaattgtc caaccatcat atgcaaaata caatggtacc agacctgaca
    12901 cccaactcca tccaacattt gacggttgaa tagctgttaa atgttgtgga agttgtggag
    12961 ctttatcaga ccaaaaagca ccaattaaag caattaggac taaaggaatc atttttacaa
    13021 aggtcgttaa agattggaat ccccctgcaa tacgcttagc aaagatattc atgagactaa
    13081 gcgctaaaat ataaacagca gctaaaataa tctgtgattc caaacttgat gattcaccta
    13141 aagtataaaa tgcagctacc catgaaacga ttgctgttaa agttggaaga tacaaaaaag
    13201 acgcaaacaa gcctaaagtt gctgctaaag ctgggctaac atatttttcg tagtaagaaa
    13261 aaataccacc actttcactc gttctaatag ctagctcaga aattgacaaa cttccaaaaa
    13321 caatcgaaaa agaacctagt actaaaatta ccatgcctaa aaagacatct ccaccagtaa
    13381 atttaaggat gtcatcaact ttaaaataaa tgcctgaacc aatcactaca cctacaatca
    13441 tagcaattgt agtcattaat ccataggtct gtttaataga attatgattc tccataatat
    13501 ctccatttct actgtacgct taaaggttaa ttataaccta atttagggct tgtcacaagt
    13561 ttatctgtgt tatttttcac cagattctga taagagaatc atattactct tatcagaaag
    13621 atgaaaacgt tttttaacca taattctcgt gcatttttac taaaaaaaag gtatcataga
    13681 gtttattatt ggaggtaaaa atgagaataa ttgttgtcgg cggaggtaaa gtcggaaccg
    13741 cactttgtcg ctcacttgtt gctgaaaagc acgatgttgt tttaattgag aaaaaagaaa
    13801 acgtccttaa acgtgtcact aaacagcatg atattatggg aattgttggg aatggggcaa
    13861 actataaaat tttagaacaa gccgaagtca aaaattgtga tatttttatc gctatcactg
    13921 atcgagatga agttaatatg atttcagcag ttcttgccaa aaaaatgggg gcaaaagaga
    13981 ctgttgttcg catgcgtaat cctgagtatt ctaatcctta cttcaaagat aaaaactttc
    14041 ttggcttctc ttcagttgtc aaccctgaat tgttagcagc acaatatatc gctaatacca
    14101 tagaatttcc aaatgcaact tcagttgaac atttcgccaa cgggcgtgtg atgttaatgg
    14161 aatttaaaat tttagaagga aataaactct gtcatacttc aatgagtcaa attcgtaaaa
    14221 aatttggtaa tattgttatt tgcgcaattg aacgcgatgg caaattgatt attcctgatg
    14281 gcgatgcaac tatccaagtc aaagataaaa tctttgtaac tggaaaccgt attgaaatga
    14341 tacttttcca taattacgtt aaaaataaag ttgtcaaaaa cttgatggtt atcggtgcag
    14401 gacgcatcgc ctactaccta ctcaatatcc ttaaaaatac caatacacac gtcaaactag
    14461 ttgaactcaa ccaagaacaa gctgaatatt ttagtcaaga atttcccaat gttcctgtcg
    14521 ttcatgggga tggtactgct aaaaatattt tgttagaaga aagcgttaca agttttgacg
    14581 cagttgctac tttaactggt gttgatgaag aaaatatcat tacctcaatg ttcttagaaa
    14641 gtatcggtat tcctaaaaac attaccaaag ttaatcgaac tagcctacta gaaatcattg
    14701 acgataaaca actatcaagc attatcacac ccaagcgtat tgccgtagac catgtgatgc
    14761 actttgtacg cgggcgtgta aatgctcagg attccaacct agaagctatg catcatattg
    14821 ctaacgatcg cattgaaacc ttacaatttg aaataaaaga aactagcaaa ttagcaaatc
    14881 gtagtcttgc atctttaaaa ttaaagcaaa atattttaat tgcagcgatt attcgcaata
    14941 ataaaacgat tttcccaact ggtgaggatg ttttaacagt gggtgatcgt atcgttgtta
    15001 tcaccttatt aaaaaatatt actagaacta gtgatatgtt agcgaggtag gaatatgaat
    15061 aaaagtatga ttcgtttcct cctctcaaaa cttttgatga ttgaagctgc tcttctagcg
    15121 attcctctaa ctgttggact tatttaccgt gaaccgcaaa gtgtcatgat gagtatagtc
    15181 attaccatga ttattctaat catccttgga ttactgggtt ctctctttaa acctaaaaat
    15241 tatcatatct acactaagga aggcatgtta attgttgcac tctgctggat actttggtcc
    15301 ttctttggcg cccttccatt tgttatttca ggacaaatcc ctaatattat cgatgctttc
    15361 tttgaagtat cttctggttt tacgacaaca ggagcaacta ttcttgatga cgtcagtgtc
    15421 ttatcccccg cgctcttatt ttggcgtagt tttacccatc ttatcggtgg aatgggggtt
    15481 ttagtatttg cccttgccat catggaaaat tctaaaaata gccatttaga ggttatgaga
    15541 gctgaagttc ctggacctgt atttggtaaa gttgtctcga aacttaaaaa aactgcacaa
    15601 attttgtatc tgttatatct tcttatgttt gcagtatttg cagtaatatt atattttgca
    15661 ggtatgcctt tctttgatag tattatcata gcgatgggaa ctgcaggtac aggtggtttt
    15721 gcagtttaca atgatagcat tgcccattat aatagtcctc tgataactaa cttagtttct
    15781 attggtatgc ttatcttcgg ggttaacttt aatctttact atcttctttt actccgaaaa
    15841 attaaagcat tctttggtga tgaggaacta aaaacctatc tccgcatcgt ggcaatagca
    15901 actttcatga ttgctctaaa cgtcatcggt atgtatgata attttcgaca aggcctcgaa
    15961 catattttct ttgaagtatc agctattatt acgacaactg gttttggcgt gactgatatc
    16021 actcgttggc ctttgttttc acaggttatt ttgctattct tgatgtttat cggtggttct
    16081 gctggctcta cagctggggg ctttaaagtc atgcgtagct taatcctagc aaagattgca
    16141 cgtaaccaag tattgtctac actctatcct aatagagtca tgtcactaca cattaataaa
    16201 agcgttctag ataaaaatac acaacatggc gttttaaaat atctaactat ctatttagct
    16261 atcttcatgg cactcgttct tgttttaacg cttgatacta atgatttttt ggttgttgtc
    16321 agtgctgcag cctcatgttt taacaacata ggcccactct taggtagcaa tgaaactttc
    16381 tcattcttta gtccattctc aaaactcctt ctttcatttg ctatgatagc aggacgttta
    16441 gaaatatacc ctgttttatt gatgtttatt cctaaaacat ggtcaaaact ataattacaa
    16501 ataataaaac ggatatatca atctgatata tccgttttat tacgtcttaa gctaaaatga
    16561 tcagggacag gatcatttcc tccgtgggct aagggatgac atcgcaaaat acgtgcaatc
    16621 cccatcagaa caccttttag accatgtttt tgaatagctt ctatcatata cgtagagcaa
    16681 gttggacgat aacgacagct agctgggaaa gctggagaaa tatttttttg gtaaaagcga
    16741 accaagaaaa tcaaaaaaga tttaagcatt aagatttatt aatagccgca ttatgcaact
    16801 gactaatttc ttttttattt aatcgtcgtg cttccccagg acggagaccc gttaaatcta
    16861 aggtaccgaa ttgtgtacga gatagcttgt ccactaataa cccaacttgt tcaaacattt
    16921 tcttaacttg atgattacga ccttcatgaa tagttaattc aactactgaa cgattttttt
    16981 catgatccac ttttatgata gtatagcgag caggttttgt tttcttacca tcgataacaa
    17041 cgccacgcgt taatggtcga aggttttctt tagtagctat tcctttaaca cgtgctaaat
    17101 aaactttatc aatttcatta cgaggatgaa tcattttatc agtaaaatca ccatcattag
    17161 tcaaaattaa aagtccagtt gtatcccaat ccaagcgacc aacaggatat atccgttctt
    17221 tgacttgtgg caacaagtca ataactgtct tacgtccttt gtcatctgag acacttgaaa
    17281 taacaccacg tggtttattt aagagataat ataccttctc ttcgttgtaa attggacttc
    17341 cttcaatttc aaccagatca ccagccttta cttgggtcgc caattcatta accacttgac
    17401 cattaatcgt gaccattcct tgcttgatta attcctcagc ttttctacga cttgcaatcc
    17461 ctgcatgcgc aatatattta ttaattctca ttgttttcct tatcttctgt attaattgaa
    17521 tccattgaaa ataacgatac ttcttcgtca actaattcaa ttgaagaggc gtcaattaag
    17581 tcatctaact gattaattcc catataatct aagaaataat tagttgtctc ataaaggtta
    17641 ggacgtccca acacttcttt ctttccagcc tctttaatta aaccaaaagc tatcagtcta
    17701 gttatagctc cactggaatt cacgccacga atatcatcaa tttcaatcct agtaataggt
    17761 tgtttataag caataataga tagaacttcc aaacttgccc gagatagact ctgattaata
    17821 ggagccttcg cataatctct caacaaagtc atataactat cctttgttac caatttataa
    17881 gtttgtgctg tctcaagcaa taatagacta gaatcgtcat cttcttcata tcgtttagcc
    17941 aacttttcta gttgctgtat taatgctgat ggtgtcaaag acaataattc tgccatttga
    18001 cgcaaactca gtccatcttc acctgcaaca aaaagaagag cttctattga gcctaaataa
    18061 gtcatttctc ttcctttctt aagataactt gactaaaatt actgtcttgt tctactgtga
    18121 tttgatgtaa ttttatcaac tctaacattg ccaaaaataa agttatcatt tcagattttg
    18181 tttggcaatc cgcaaacact tcttgtaatg tcgttttctt tttaagatta aaatgtcttt
    18241 caataacgat catcatatct tcaatacggt agtcttcttt ctcaattacc gtttgactat
    18301 ttgacaattc tttttgtttt tgggacatca tttgagaaaa agtcaaaaaa agatccatca
    18361 ccgatttatc gtgtagaaga attgcatctt caaagataac ttcttgtttt ggttttgaaa
    18421 aatatttggc acgttcttga tgctgattag ctaactcttc acttagcact ttaaaacgtc
    18481 tatattcctc taattggctc aacaattcca tttctggatc atcttcaata ggatttgact
    18541 ccactacttt aggtaataag ttacggcttt ttatcaacat cagctgactt gccataagca
    18601 tatactcacc agctacttct agacgcatcg cctgtaatgt tgctatataa gcaaggtact
    18661 gctcaataac ttccacaatc ggtacatcgt atatatcaac ctcatattta gatacgaggt
    18721 gtaagagtaa gtctaggggc ccctcgaaat cttttaactt aatatccatt atctataata
    18781 tctctctaaa gtgataggag tttttaagcc tagaaggcga ctgagctccg tcatcgtttt
    18841 gccattttct ttttgcttta gaataaattg ttctcgcaga agttgtgcag ttagttgttg
    18901 ctcattcttc tcatttaaga aatctgttaa ttgattaaaa aaccattgtc tagaataagg
    18961 cttaccgtta tgctcaaaga ctaagtcacc atcttcctct aaagattcca aaagaaaagg
    19021 aagcaagtct tctggaagtt ttaaaatacg cttcatctgc gatttttcaa tacttaagat
    19081 attaaaagtt gtatcaaaat cagccttctt caaattagca atttcagccg gagttaaacc
    19141 taaagataaa atcaacaaag caatcaaacg accactcgga taatcagtat cttgatagaa
    19201 atttgataaa tccattattt gagactttgt ctgagccaaa gtgatttttt cagtctcttg
    19261 tagtctgtaa aattccttca gcgttcctct attatataaa aagaaaagaa attgattgac
    19321 tgcagataac tttcgcttac gagctgttaa cttaaattca cttagagact gttgatacaa
    19381 tgccagtttt tcagaattta catgtccacc aattatttta taaaattgct ttaaatcata
    19441 gtgataggac ttacgcgaat tgagagataa tttcttactc tcaataaaat tattaatatc
    19501 atttatcatt gtttttagga gtgatagtat attcgtctgt aaaatcatgt aggaggctat
    19561 taatagcttt taaaatcgat ttacgggtta taatgcctct aaaatcattt tgatcactga
    19621 taacaggtaa aaatggataa tctacaatct tatgcataat tgctgtcaga gtagctgcct
    19681 cattaatcgg ctcgattttt gtattaacca tctcaacaat atcagtctgt gccatctccc
    19741 aatctgttaa ttgaccttta gattggtagc ccataatgtc tgagatacta atagtaccta
    19801 catatttttt ttctttagtg ataactggta cacgtgaaaa accattactt gctaatagta
    19861 gcataacgtg gtctgcatta tgcgtatcca caaaaatagc aacgtcttca gctggaatta
    19921 gatagtggtc caaatgactt agcaaaaacg actcaaattc tttagcaatc atcttgaaaa
    19981 ttcctttgtc aagttaggat agggttggtg ttggcgagta taatactcta ccaaaacctt
    20041 actatctgtt atagttacaa cagcgtaaag cttctcattt attggtcctc ttggctgcaa
    20101 aacactacct ggattgataa aaatagtttt accatttttc caagcatctg cacgatgaag
    20161 atgaccatat aaacaaatat ccgcgtcttc ttgctgagct aacaaatcca atttatccca
    20221 agtaaaatta atctgatgga gatgaccatg ggtttgaaca ataacagtat tatctatttt
    20281 tgtaaccaaa acttccggat aacctgaatc ataatcacaa tttcctgtga ccacatgaat
    20341 gccttcccat ataggatctg aactaggaag ttcggaatcc ccattgtgaa aaataacatc
    20401 cacttttccc aaataatgat tctttatatc ttttacaatg tcacgatctc cgtgagaatc
    20461 actcattaca actaattttc taattgccat tttggaaata cctccatcaa tttacggaca
    20521 gcttgcccac gatgtgatag attatttttt tcttgtgcgc tcaattcagc tgctgtccta
    20581 ctaccttctc caacaataaa gagtggatca taaccaaagc cattttcccc cttaggttcc
    20641 gtaccaatat aacctggcca ctctgcttca actaccaaac tttctttatt tggagctgac
    20701 acgactaagg ttgtatgaaa ctgtgctgaa cgtctttctt tgtcaaatac catggctaat
    20761 tcatgcaata atttggcatt attacgagca tctgtcgcgt caggacctga aaaacgagca
    20821 gaccataccc ctggtaaacc acctaaagca tctactttta atccggaatc atctgaaatt
    20881 accattttcc cagttaattt tgaaatagtc tctgctttaa gtcgagcatt ttcttcaaag
    20941 gtcataccag tctcttcaac ttctggtaaa tctgggtaat catttaaatt ttcaactttg
    21001 agtcctaatt tcccaaataa ttctctaaat tccttcgttt ttccttcatt gtgagtcgca
    21061 attaatatgg tatccccaaa attattatca ttctttaaat caaagaattt ttctaaagaa
    21121 gtgccctctt taggtaagtg aactaagaac aactgatcat cttcactaac taagatggcc
    21181 cctttatgtt gagtcacttt gatgtgcctt gaatactctt gattaatcat atcaacgagg
    21241 aatgctaata attgaaaatc cgaagtcatc tgcacaacgt gaagctgtag accttcaact
    21301 ttcaaagttg ataatagacg atgaaaaaca tcttggatag cttcgtaaga ttcatcatta
    21361 ccaaaacaag taaaataatt aaaagcttgg aaactaccta aaaaccaatt gccttcagtt
    21421 ttactttcaa aaattgtttt tgtcataata ctatacgctc cacatttatt tcttgactaa
    21481 gccattgttc tgcaatttct ttaaaacctt ttgggctggc ggttgtgtaa aagtgatgac
    21541 caccgtgttt attttgccaa ttatggttta tctcaaaata gttcaataaa acagaaatat
    21601 cacgaacggt ttctgcgcca ctatcaatta atttaacctc agcccccata acattttgaa
    21661 tgatgggacg taataaggga taatgcgtgc aacctaaaat taaagtatct aatttaccaa
    21721 ctaatgggga caacgtttca taaaccacct ttttggctaa actagaagac atctgatttg
    21781 attccacaat tggaacaaat ttcggacaag caagggatac cacagcagta tttggagaca
    21841 aagcttgaat tttttgacga taagcatctg atttaacagt catgggagta cctataatac
    21901 caactttccc tgaattagtt gatttgatag ctgcgctagc tcctggtaaa ataacgccta
    21961 aaacaggtat gtctagtttt tctttaattt cttgccaggc aactgcagtt gctgtattac
    22021 aagctataac aatcatctta acatttttag tcaataagaa attaaccatc tgccaggtaa
    22081 actctctaat ctgttgagca ggtctaggac catacggagc tctagcctga tctccaatga
    22141 agattacttc ctcttctgga agttgacgga acatttcctt aacaaccgtt aaaccaccta
    22201 caccagaatc tagaaagcca ataggcctac tatccatttt tattcctcca atattagtga
    22261 ctaaaagagg ctggacaaca gtcctagcct ctctatacaa taaccaatca gtcaaatcta
    22321 cacttgacca agtttgccca aatcttgtct agaaatctga tggactatca ctcactattt
    22381 catcactaaa cgcttcaatg ctaaaacact cttatttttt agttttgata gctgtttttg
    22441 cgtgttttac aatattacgg tatgtctgtt gaactttagc ttcactaggt ttttgtccca
    22501 tttgactcat catttcacga atagcatccg gtgttaaacg agggtgctct ccaatctctt
    22561 tttcaatttg tttacgagca ataaaaatgc ctcccacgag accaccaaac aaagcgacga
    22621 taattaataa aatccaaata gttgttgaca tctattcatt ctcctcattt atttacatga
    22681 ctcattatag caaaaaaaga aagagtttac aaatatatgt aaacaaaatc aaagcctcta
    22741 tcgtccttgt cacgtcaata cactattaaa ttttcactag agcatgatta agcacatagc
    22801 cgagtccttt atcttatcct aagtgttttc cccaactttt gtgtcatcca tttccaaatg
    22861 aagagagcta tataaaaatc taccaatcca tgtagcaaag agcctagccc tatcaaagaa
    22921 aatagtgacc ataaataagt tgaactactg ttagaagtca ttgtgattat ataaacagta
    22981 ataaattcga gcagaccatg aattatgtta atagctacag caaataacaa tgtttgccat
    23041 ttactcatca ataaagaggg ctttgactta atcaagaaag ccgcaattaa tgcaaatccg
    23101 atatgtgata aagctctaag aacgattaca atcggaaaac cagctagtag aaatccaagt
    23161 cctgtcccta aagctactaa tattgcaact ggaacagata taaaaatact taaaaataaa
    23221 ggtacatggc ttgctaaggt gaaagaagca ggcccaataa ttattttagc aggcatgatt
    23281 ataggaatca tgattgcaaa agctactaaa atagaagtat aagaaaccat ttgtgttgtt
    23341 ttttttgaca tttttatctc ctttgaagct attatacaaa ataaccttac aggtgtaaag
    23401 acacctgtaa ggttatttgt ctatgtataa aattcctgct ttttttagag cctcacatat
    23461 ttggtcaaaa actgactgat cttgacaaga aatcatatgt gtatgtaaac catctgtcaa
    23521 agaggatagc aattctgcat tggaaacttt taatttagca gtaaaattat caatgtcttt
    23581 ttggctttga atgtttaagg gagcagtcag catcccataa atagggtgct ctacctccac
    23641 attcataatg attccttgat accgcaaaat aatttccagt tcttcttctg tctgttctat
    23701 gccatgctga caaaccaatc tagcagtgaa ttgatgagta cttagagcac tggacatgag
    23761 gtatcctttt ggggtagaaa taatatcaca ttgttgtgca cgcaataagg caatatctcc
    23821 aacaatcacc tgcctactaa cactaaaaat ctttgctaat gtgcttgccg atatagcttc
    23881 ttttgttcct tttaaagtcg ttaaaatatt ttctcgacgc tcctgtgcct tcatataaca
    23941 aattccttct ttggtgttat ttcacaatta aaaactggct agaatatcta accagttagc
    24001 ctcaatcatt tcgtgcaaaa agacgaagaa tatttaagaa tagattaata aaatcaagat
    24061 aaaggcttag tgccatcgaa actgcccaac catctgcgac ttggccgcct tggctattgt
    24121 agacatattt aatcatttgg ttatcgtaag caatcaatcc agaaaagatg atgacacata
    24181 cgatactaat gatataactc atccccccac taccgataaa taaattaaca agacttgcaa
    24241 ttaaaatccc aattaaagca gccattaacg ctttacgcat acctgataaa tcttttttag
    24301 tcttagctcc aatcaaagcc attgcaaaga aaacagctgc agatgttata aaagcttgaa
    24361 gaactgttgt ttgcgtgtac cttgcgataa tgaaacttaa agtaaaccca ttcaaagctg
    24421 aataaatcaa aaataatggt aatgcagctg gtgtgtttcg acgcgccgct ccactagcaa
    24481 caaagactaa aaccaattct aagatgattg ctccaaaata caaccctggc attttttgca
    24541 tgatggcaat catattttgt ggaaacatat acagcataat agcagaaaca gcagctgaca
    24601 aacctactcc tatccctaca agaccgtaaa tcttagcaaa aaattgattc agccctgaat
    24661 cagattgtgt gtaaatgacg ttatcattca tatgtatttt cctattctaa attttaatga
    24721 ttgtgactac ctaagaattt gcgactaaaa cggataggtt tcttaaaaat acgtttgagt
    24781 ggacgagaag cctctcgtac tgcccaatac ttatcaacca tagtcacaat ataactttct
    24841 ttataacgtg gtggtgcaat agtcgctccc cacatgtgtt tcaaaataat atcttcttct
    24901 ctagcgttta aatcagttaa ttttcgcgca ttacgaaccg ctatccttgg atgaacccag
    24961 gcatggctct tgttaaattt cgttacacgc caatcataat aaaagaaatc atgtagtaaa
    25021 ccacctcgag cagtactact cgcatcccaa ccaaacttct tagcaatttt ataacttgta
    25081 taactaacat ttatagaatg ctctaagcga gttgagtgat gatgttggac aatagcttcg
    25141 agtttttgga aacgtggatg gtcaataagg tgaccgacca aatccatgaa gtccttatcg
    25201 tcttgatact ttttcattat aatcacccct ctggggatat tataacatat gaacttaaat
    25261 taagctgaaa atgactactc ccgcagctac tgctacatta agactttctg cttgtccagg
    25321 catttcaata tgtgctaaaa catctgcttc ttctgtcata gttttggaaa ttccctgtcc
    25381 ctcatttccc atgattaaag caaaataatc atatttaggg agatttttat aatttaccga
    25441 ttgttcagat aaagttgtgg caatcatagt tatcccttct gctttacaaa agttaacaaa
    25501 ctgctctatt tctaccctaa atactggtaa atgaaagtga ctgccttgca tagaacgaag
    25561 tgttttttgg ttatacaaat cagcagattt ctgagataaa aaaacagcat caaaattcgc
    25621 tgcatctgca gttcgaacca ttgttcctag gtttcctgga tcctgcacat cttccaatac
    25681 aagaaaacgt ccttttttaa tatctgtcca tcttgtctct tgaaaggcaa tctcagctac
    25741 aatcccttga ggagtctgtg agtcagttaa ttctttcaaa atatcatctg taacaacaat
    25801 agctctctcg ggttttctca aacgatctat tgccgtttct gtcacaaaaa tatttaaaaa
    25861 ttgtgcacca tatttttcag cttcctcaaa taaatgccag ccttcaataa gatatgaaga
    25921 tttccgatat tttttttgta acaacttttt cgttttttta atgagattat ttgattttga
    25981 agttataata gtagtcataa acctattata tcagaaaaga ggtcaagtta tgaaaaaagt
    26041 tcatttaatt gtctcagggc gcgttcaagg agtcggattt cgctatgcaa cttatttctt
    26101 agctttagaa ataggtgata tttacggtcg cgtttggaat aacgatgatg gaactgttga
    26161 aattttagca caatccacag attcaaataa aatgacacaa tttattcaaa aaattcgaaa
    26221 aggaccttca aaatggtcta aagtaactta tgttgatatt aaactagata acttcgatga
    26281 ttttaatgac tttaaaatga ggaattaaaa atcattctgc ggaagtattg tttaattctt
    26341 aaaaaaaaag taaaatagat aggtatatta aaaattagag gaactaaaaa cattgaaaaa
    26401 aacattgaaa cgtatcttat tctctagctt atctttgtct atgttattat tattgacagg
    26461 atgtgttagc gttgataagg ctggaaaacc ctatggagtt atctggaata ctcttggagt
    26521 gcctatggcc aacctcatta cttatttcgc tcaacaccaa gggcttggtt ttggtgtcgc
    26581 tatcattatt gtaaccatca tcgtacgtgt cgttatctta ccacttggac tttatcagtc
    26641 ttggaaagct agctatcaag ctgaaaaaat ggcgtacttt aagcctttat tcgaacctat
    26701 caatgaacga ctacgcaatg ctaaaacaca agaagaaaaa ttagcagctc agacagaatt
    26761 aatgacagct caacgtgaga atggccttag catgtttggc ggtattggct gcttgccatt
    26821 actcattcag atgccatttt tctcagctat tttctttgct gcacgctata ctcctggagt
    26881 ctctagtgca accttcttgg gacttaactt aggtcaaaag agtctgacac ttactgtcat
    26941 tatcgccatt ctttattttg tacaatcttg gttgtcaatg caaggtgttc cagatgaaca
    27001 acgtcagcaa atgaaaacaa tgatgtatct gatgccaata atgatggtat tcatgtctat
    27061 tagtttacct gctagtgttg cgctttattg gtttattggt ggtattttca gtattatcca
    27121 gcagctagtt acgacttatg tacttaagcc taaactacgc cgaaaagtag aagaagaata
    27181 tactaaaaat cctcctaaag cctacaaagc taataacgca cgaaaagatg ttacaaattc
    27241 aactaaagcc actgaatcta atcaagcaat tattacatct aaaaaaacaa atcgtaacgc
    27301 cggaaaacaa aaaagacggg gataaaacaa gcatactaaa taatggtgtg ttgtcctaaa
    27361 cagttaaaga ttattatgta attcaagtaa aaaagactta gttctatgtc ctacatagct
    27421 aagtcttttt agttttgcct agcttttcat ataaaaagca ccctaaaagt cagataaaaa
    27481 tctagctttt aaagtgcaag taatctagct aacagactac cttattaata aagtaatcgt
    27541 ttttcccacc agtcttgaat aattgcataa agcgtacaaa ttacccaata gataagggct
    27601 actaagatat aggcggtcat atagtcggag taggttcccc caactatttt agcattatta
    27661 aagatatcag gtaccgtaat catcgcagtc aatgaggaac ccttgaccat atctagcaaa
    27721 acattactta aagaagggag tgcaattctt gttgcttgcg gaataataat gcctctataa
    27781 atcgtcggtg tttttaaccc taaagctcta gcagcttccc actgaccatg atcaacagct
    27841 aaaagagctg ctctgataat ttctgatata tatgcactag acatcatggt aaaacctacg
    27901 atagaagcag tcacagcatc taactgtatc cctataaaag gcaaaccaaa gtagataaga
    27961 aagagtagaa ccattaaagg aatcccacgc attaaggaaa tatgaatatt agctagatac
    28021 ctcagtaagc ggaaatgaga catcttcatt agagctacaa agaatcctac gatagctcct
    28081 aaaataaagc caaccaagga taatccaatg gtatatggta acccctgaat aacacttgga
    28141 aaagccttaa cagctagttc caaattaaaa atggcatccc agtttatcac ttatctactc
    28201 ctctcgaaat tttttattta actttaatta ctggaatttt atccgttcca gcaggttctt
    28261 ttgtgagatc ttgaccagca taatattttt cagagatttt tttcaaagaa ccgtctttct
    28321 tcatatcctt gatagctgca tcaatttttg ttttgaggct cttatctttt ttactcatca
    28381 caataccttg ctcagtcgga ttatacttca catcacccat ttttactttg atatctggat
    28441 attgttttgt tacatatttt acagcaatga cttgtgtgta gtaatcattc ggaatgaaat
    28501 ctgtacgtcc agttgataca tctcgaaggt aaacgtcatt agttacattg tcataaataa
    28561 caggttcagc accttgctgt tttgcaattt tcatatattg cgttccagcg ccaccaccag
    28621 cttttttacc tttccagtca gataaatctt ttgctgtaat ctttgaagaa ccatctgcac
    28681 gtacaatcat acctccaaca gaatatttat aaggttgaga gaaattgtat tttttcaaac
    28741 gctcgggagt tgtgtcaaag ttattgacag caacatccac cttaccacta tctacagaag
    28801 taaatgattc agcaactcca acttcaacaa acttaacttt tatcttaagt tttttagcaa
    28861 tcgctttcat catatcaatc tcataaccag tcaatttttt atggtcatca taataacttg
    28921 ttggataaag cgttgcaggg gtagcaacct taaggacacc atctttttta atattgtccc
    28981 atgttgtttc tttgctagat tgaccatttg agcaagctgc taatgtcatt gcagataaac
    29041 cgactaaacc aagtaaaatt gtctttttca taagtactcc tgtagattaa attatattaa
    29101 aatggtttgg cgtactctat cgaaaattaa atttaatgaa aattaataca taaatttacg
    29161 ataataatac aaccattgtt aagagtacca tattgtatcc gttttcgcaa aaatattgtt
    29221 tacttttcaa attaatggta aacaggccct ttgaattgct tctctttttc aaacaactct
    29281 gcaacagcaa gcaataaatc ttctctccct ttagccgctg tcaattgtac acctaaaggt
    29341 agcccctctt tagttagatg agttggtatg gctaaagaag gttgccctgt caagtttggc
    29401 tgatgtgtaa aaggtgtcca ggccattgag tcttcaaaca ttttccaaat taaatcctgt
    29461 tgttcactta ctgagaattc tcccatatgc ctcaattgtt tctggagtgt ctcgtctaaa
    29521 tcaaattgtc catgaaaagg agctggttga ttagttgctg ctgttaaaat taaatcataa
    29581 ttttcatgaa acctcgccat ggtagctgca aattgatccc atgaatctaa gagtttactg
    29641 taatctttag ccaataccct ttgtccactc tgatacatag cccaggtcat taattccata
    29701 tcgctaaatt ccattgaacg ccctaaactc ttttcgatgt catcaaacat tgctgctgtt
    29761 tcaacgctat tcatcataca ataagttttc atagaatgaa taccatctaa aggttgttct
    29821 actaattcta aatgattccc tttttgagac agaaaatctg cggcttcttt aatagctgct
    29881 ttagcctcac ttgatacctt tgtttttaag ggagaatcca tcaatactgc tatttttagg
    29941 ggtttactaa cgctaaactc gaaaagactc tctttgctga gttttttcag gggaaatggg
    30001 ctttctactt gatatgattg taaataatat aatagtcgtt ttgtatccct aactgacttt
    30061 gttaaagcaa aatgactaga agccccttgc caacctctat aggatgaagg tccaacaggt
    30121 atacgtcctc gactcggttt taaaccaatc aaaccattaa aacttgctgg aattctgatt
    30181 gaccctcctc catcactagc tcctgcaatt ggaaccattc ctgaactaac agctgcggct
    30241 gcaccaccac tagagcctcc agcatttctt gaatggtcaa atggaagatt aacattcccg
    30301 tgaagttgac catcactaat attcttaaaa ccaaattcag gggtatttgt tcttcctaaa
    30361 ataataaacc ctaatttttc aaaggactgc actaaatagt ccgtttgttt agcatgatag
    30421 tgtttaaata attgtgaacc tgaggttgag agttgtcctt tttgattctg tcccaaatct
    30481 ttcagaagaa taggaactcc tgcaaaagga gcatttgatt catttgcgta tttgactgct
    30541 tgacgcgcct cattatattg cttgctgaca acagcattaa cgcttacatt ctgttcctcg
    30601 atcttgtaga ttgcttgctc tactaattct tgcgacgaaa tcttatgctg ttttatcgct
    30661 tgtaccatag cggtagcatc tttaaaaacc atataaacac ctcataaaat gtatttttat
    30721 tataagataa caaaaaatac ttaaaaaaaa tagaataacg tctagactct agatattatt
    30781 ctatgattta tactattaat ttgttttttc aacactaatg atttcaactt gatagctacc
    30841 agcaggtgat tcaattgtag ctaagtctcc tgttttttta ccaattaaag catgagcaat
    30901 tgggctttca ttcgaaattt ttcctgaaaa aatgtcagct ccagcagcac caacgatatg
    30961 gtaagtatct ttatcatttg taccaacttc ttggacaagg acagttttac caattgcaac
    31021 ctcatcttta gcaactgcat cactatcaat aatttcagca taacgaattt ttgtttctaa
    31081 aatttgaatt tgaccctcaa caaatgcttg ttcgtctttt gctgcatcat attcgctatt
    31141 ttctgataag tcaccataag aacgagcaat cttgatacgc tctacaactt ctggtctacg
    31201 aactaatttt aactcttcaa gttctttttc aagttgatct ttttccactt gtgtcattgg
    31261 gtaagttttt tctgccattt tatttctctt ttcttttcat tttttcaagt caaagatata
    31321 aacatgatga ctcgcaccat gtttataagc ttagttaatt tgactattga tatatttttc
    31381 aacgttagcc ttgtgtgttt caaaatcttt agcgtagtat acatttccgg tcttcacatc
    31441 agcgacaaaa taaaggtaat ctgttgaagc aggttttata gtcgcttcaa ttgctgagat
    31501 acttggacta tcaactggtc caggcatcaa accagtatta gtatagatgt tgtatggtga
    31561 tttaattgaa gtattaattt gagcatcttc agctaaactt gttttatcac ctaatttccc
    31621 catagcatac aaaattgcaa tgttactttg aagagcttga ccggctgata atcggttata
    31681 aaagacactt gctattttac ggcgatcttc gtctgttgat ccctcttttt caacaagaga
    31741 tgataatgtc aaaacatcat ttacagacat attcttcgct ttaatagtgt tatagtaagg
    31801 tgccatctta gtattcattg ttgaaatcat atcttcaaca agtccttcta aggttgtatc
    31861 tttataatag ttgtaggtag ctgggaataa atatccttcc aattgataaa tagcttttga
    31921 tttatcaggt aaacttccta gtaaatttgg atatttagct accatttttt taataaacgt
    31981 ttcatcttga acaagtttca agaaatcatc agctttataa ggtgtacttg tagtctttgt
    32041 atcaatttta ttagactcaa tagctttagc aatctgttta atagtgtaac cctcggtaat
    32101 taatatctta cctaaagctg gttttgtcgg ttctgcagtt ccgccttttt ccaattcagc
    32161 agcaatctga tctaaagtca tacttttttt aagattgtag tatccacttt gaaaattact
    32221 atagtttttg aacttggaat agtaattaaa gactgtggcg ctcttaatga ctcctgcttt
    32281 ttctaaaatt tgaccaatta atttattccc agatcctatc ggaatttcaa cttgtacaaa
    32341 tttatttgaa ttggaatcaa caggtttaat agcactatct acaaaacgat aacctacaaa
    32401 tgctgtcaag agcaatacaa gtatgataat tgatatcaga atacgtgcaa ttcgttttgc
    32461 cacattatct gctttctgac gtctttcacg cttgttatgt ttgttcttgg ctgacaaagc
    32521 tataccctct tcttcttttg acaaatcctc tactcggata ggttcctttg ctacgggctc
    32581 taactctgat gtctcaccaa gttttacatt ttcaccttct gtaggaatat ctgttgctct
    32641 cacaagaaag atttcttccg tttcttgatt aggcacagca gttgcaacat cagtcttatc
    32701 tgcttttaaa tctatgtcag gtacattttc agttgtccta aattcactag atttggagcc
    32761 gctatcaagc tctcttgaag gttccatccc ttgattgata acttgttctt cttgggaagc
    32821 taatgcttgt tccgcctgac gtctttcttc ttctcgagca gcttctgcca gaagtttcgc
    32881 ctcaatccga gccttctctt gctcttctaa gcgttgctta gtctctagcg cctttgctct
    32941 agcttcttgt tcgtctttca aacgttgcgc ttcataatct gccatcaatt gtgctgttct
    33001 gcgagcagct tctttggctt cttgctcttt ttgatagagt tcttcttcac gcaactttct
    33061 caaacggtta gcttcttcta actctgccag aatttgttct ttaaaacttt tctgatcatg
    33121 gttagaatgc tgatcgtcat taaattcggt caaagagatt tcctcctaat aataatcaca
    33181 ggcaattata tcttaaatag tctataaaat caacaaaact ttgacaatgt tatctacttt
    33241 taacaaattg atcgtgttgg attattccaa atcatattat accaaagcga accaccatgt
    33301 tgggagtcag attctccctc atctttaaat ccattcattt catagaagga aattaaatca
    33361 tcatggcaag ttaaacttat accatctcgt tcttgagaaa caaccaggtc tttcatcgct
    33421 gctaataatg ctgtaccaat cccctgacct ttaaaatcag gatgtattga tagactagta
    33481 attcctatga aaccacctac ccttacaggg aattcagaaa ctttatggaa taaatcgtcc
    33541 gttaagtacc gacctttaat aaccggtcct tcaatgtaac ctgctaaacg accattaatc
    33601 tctgcaacta aaaaagtatc agtcattatc gttaaacgtt cttgcattgc ttttttactc
    33661 gcagcctctg cttctgaaaa atttatttgt tcaatactaa tgacttgctg taaatcctct
    33721 aggcaaccat ttctaataat catactcatc tccttaattt ctttatgaaa ttctcttgat
    33781 aaagtcagat agaggactga gaaacttctc tcagccctta ccatcaactt attgtgtatt
    33841 tttagttagg ttagctaata attcttcaaa agagcgctca tacaattgaa tgtctccagc
    33901 acccataaag acatagacag cattatcatg attgagtaaa ggcgagacat tttcgactgt
    33961 cactaaatct gagtgtttga caatcttagc agctaaatct tctaccttca cctcaccatt
    34021 atctacttct ctagcagaac catatatttg agcgagataa acgctatccg cttgactcaa
    34081 ggcatgggca aattcgtcta aaagagctat cgtacgagtg aacgtatgcg gttggaaaat
    34141 agctacaatt tcttttgacg ggtatttttg tcgagcagca tctaatgtcg caataatctc
    34201 agtaggatgg tgagcaaagt catcaataat gacagtatcg tcaataatct tctcagtaaa
    34261 acgacgcttt acccctgaaa atgtcttcaa atgctcagct actaatgcca tatcaattcc
    34321 cattatgtaa aggttagcaa taacagcagt tgcatttaag atattatgtt taccgtatgc
    34381 tggtacatga aactgaccaa tttcttcttg gttatagaaa accttaaagt cagaaccatt
    34441 aacagttcga gtgatgtctt ttgctataaa atcatttgaa tcttcaaaac cataataata
    34501 tattggtgcc tcagaagtga tttcatgaag ttttggatct tctccataaa tgaataaacc
    34561 tttttgaact tgcttagcat agtcattaaa ggcattgaat acgtcctcta ggcctgtaaa
    34621 ataatcagga tggtcaaaat caatattggt aataattgag tattctggat ggtacggcat
    34681 aaaatgacgt tcgtattcat cagcttcaaa cacaaagtaa ttagcattag cagaaccacg
    34741 tcctgtacca tctccaatta ggaaagaagt gtctgtaata ttttttaaaa catgagctaa
    34801 taaacctgtc gttgaggttt ttccatgtgc cccagctaca cctagactag tgaactgacg
    34861 cataaaatct ccgagaaatt catgatatcg tttaaaatga tagccctttt caataacata
    34921 agccaactct tcattgttat ctggacgaaa agcatttcct gcaataatct ctaaatcctc
    34981 actgatatta ttcggtgaga aaggtaatat agttacacct gcttgctcta aaccacgttg
    35041 ggtaaaataa tatttgtcaa cgtcacttcc ttggacgtta tgtcccattt gatgaagcat
    35101 cagtgctagg gcactcattc cggatccttt aataccaata aaatgataag tttttgacat
    35161 ggtctctcct taattaatgt ctttaaaacg tgtcagattt aattcttgag ccttggctct
    35221 ttctttatga aactgattgt cttgtttatt gtaaacttga ctgcgtttca aaaaatcaaa
    35281 attatttttc tttgtttttg ggttcggtgt attatcaggt tcttgaaata tgattggaat
    35341 ttctgctaat atatagcttt cctgctgtaa acgatcagaa agatgactta gttcagtcac
    35401 ctttcgctga gtttgttttt tcggtactgt tgccttcaat tgttgctgaa aagcttcctt
    35461 tgatggcgcc ttaaattcct gtttcgcata gtaagcttgt cgtttctccc gaacagacct
    35521 ctttgcctct tgtcgagatt tgctcaactc tgtcgctttt tttacaaaac tctgcggtgg
    35581 tacaggatca ttttcaatta ccttaggatg tgatatttct tcatgttcat aattgctttt
    35641 gaccatgctt tgatatgttt tttcttgata aaagcctcga atgttgttaa ttaaatcttc
    35701 attatcataa agattcatct ttacaagcgg agacattact ggttcatcat ctgctatcaa
    35761 cggaaatcgt ctagagcctc tattcacatt atcacttcct ttctacaaca gacctattat
    35821 aaactaattt ttctgttttt tcaaagtttt caactaagta aagcttatca gattggtatt
    35881 tttcattcat ttatataaaa aaagaaagcg agagaccact ttctttttta tattatagtc
    35941 acatcatcat atttatttag aaactccaag tatctctcga atatcatcta cgctcatgct
    36001 agcatgggtt tcatttccat ccagaactgt tgtaaccaaa tgttttttag tttcttgcat
    36061 ttctagaatt ttttcttcga ttgtgcctct agtaatcaag cgataaacct cgacattctc
    36121 tttttgcccc aacctatgtg cgcgactaat cgcctgcatt tcaacagctg gattccacca
    36181 caagtcaatc agaacaacgg tatcagctcc tgtaaggttg agaccaacgc caccagcttt
    36241 taaagaaatc aagaaagcat cttttgatcc tgcattaaat gctctggtca tttcatgacg
    36301 ttcatttgct ggtgtagaac cagtaatttt ataagttgta agccccatag ctaccatctc
    36361 acgttctgcg atatctaaca tacccctgaa ctgagaaaaa atcagagcac gatgtccatt
    36421 ttccttaatt tgtgttaaaa gttgacgtag gctttctaac ttaccgcttt caccatcata
    36481 atccataaaa agacgtggag tgtcacaaat ttgacgtaat cgtgtgatgc cagataaaat
    36541 ttcaatttta cgacgattta agtcagcgtc actagaatgt tggatacttt cttgaatttg
    36601 gcgtaattgc gccaaataga taactttttg actgtctgtc atttcatttg gatagttcat
    36661 ttcaatcaaa tccggtaatt ctgggagaac ttcttctttt cgacgtctca tcacaaatgg
    36721 tttgatataa cgagcaactt gtttagggtt caacttcaaa aactctttct taccaggtaa
    36781 taaaccaggt aaaatgatct ggaaaatgga ccaaatttct aacagtttat tctcaatagg
    36841 tgttcccgat aatgcaaaac agtttttgac ctcaaaagat ctcaagctat gggctatttt
    36901 agtttgagca tttttcataa cctgtgcttc atccaaaatt aaataatcat aatttccttc
    36961 agaataagtc tcaaaatcct gcctaaatga tgaataactt gtaatgatta cttgatgacg
    37021 ttcagcaata atttcatctc tacgaatctt agaaccatag gcaactgcta catctacgtc
    37081 tggcgaaaat ttgtggaatt catcttgcca attataaatt aaacttgatg gtgataaaat
    37141 aagcacacga gaatctctag ttagttttgt acttaaaaat gaaatcgttt gaagtgtttt
    37201 tccaagcccc atatcatcag ctaaaatccc tgcaaagcca taatgatcta acatacttaa
    37261 ccaacggaca cctgtaagct gataatcgcg caaggaagct gaaacagata gaccaggtat
    37321 tttaaacttt tctgggtgtc tcaaatcttc aatcaattgg taaaaatgtt gtgaaaagct
    37381 aacattatct tgatttgcaa acagttctga taattgaaat gctgcaatat tatctaattc
    37441 aatatgccca ttctttgctc gtctagctct caacccttgc aaagttgcac ttactttctt
    37501 agtttcttca tcaaaaataa ctaattgccc tgatttattc acaaaataag gattattgtc
    37561 aaacaaagct accagagctt gatcaacatc gttttccagt aaatcagaga aatcaaaaga
    37621 aatatctagc aggcttccct tagcttcaat atcaatttgc ggtcgttcta tgagacgata
    37681 actttccaat tttgcggata agctcactgt tcccattttt ttaaattgtg gaagcatata
    37741 ggtataaaaa tcgtataatt cttgtgcact gtatgctgga tggctggttg aaaagtgggg
    37801 tgcaaagcca tatttttcta agagtttaaa tactttttct tcatgtttga agtgactagc
    37861 aaatgttaac tgttctagtt cttgacgatt atgaacagtt aaatcattac cataatcaaa
    37921 taccatttgc aataaaattt cattttgact attaatatcg aactcaaaat ttaccttaaa
    37981 atcatgaata gagaagctcc gtggagcatc cactagtcca atctccttaa aatccagtaa
    38041 atgagctgct aacttatctt gatcatctaa atcaaaatgg atatgttttg ctaaatctcc
    38101 ctcaatggga agactacgaa tagccgttac catcttttct tgcttcaaat taagatggta
    38161 gaaagtatcg cgataaaaca aataagaatt cgcaaatagt ggttttaagt ctttctcttt
    38221 gatttctaat tcaattgatt gcctatggac aataacctta aattgataaa gatgatcctc
    38281 accttctaat tccttaaagt agaggtgatt aaattgatga aaatcacttt caaatgaaaa
    38341 attttctaaa gcattcatca aagtaacacc ctcttcaaaa aaaccacgag tgagactaag
    38401 gtgacgacct tgatttggta aaataaactc tagatcaatt ttgcttgaat gacttggaat
    38461 gagcctccat aaaaattcta tcaattcttg actggtctca tcaaactgta tcaaagacaa
    38521 agtttcaaag tattgttttc caatctgata ataagcttct tttcgaacag tatttaaaaa
    38581 ggctttgatg tcacgaataa cataacttct atcatcaggc agtcgtctaa tttttaaagt
    38641 ccaccagata tcattagcat aaggattttg ttctccttga gccgataatt gatattttat
    38701 agtatcatct tcattaataa ttagactatc taaaaaaaca ctaccaaaag aggttttctt
    38761 cttagtttct tgttgacttt cttgttgaac ttgaacttta gataagattg ctttaccttc
    38821 tggatcgttt ttcaagaaat gctccagagc agctaaatgt tgacaatagc cttttcttgc
    38881 aaaaaaatca caagaacatt ttgtttcttc tgttactaat gaatattcaa tctgacaatc
    38941 cccaacttta gcttttaata gattaccttc ttgagaaatc aaggacacca aaccttgttc
    39001 gtagagttct ataccttgat ttcgaatacg tcctggaatc attctactca ttatgctacc
    39061 accttcatct tatcccttaa ttataacaaa attcaagtcc aatttcttgt aaaaatacta
    39121 aattgattgt tttttgatac agtaacaaat aaaaaaggct aaaacaactg tcctaacctt
    39181 ctatttcaat ttcaacaata agatgttatt tacgtttacg tgctattaga ttaattggtg
    39241 ttccttcaaa aacaaatgct tcacggattt gattttctaa gaaacgaaga tatgaaaagt
    39301 gcatcaattc ttcttcgtta acaaaaacaa cgaaagttgg tggtttaaca gcaacttgag
    39361 ttgcgtagaa aatcttaaga cgttttcctt tatcagttgg tgttggatta atagcaatag
    39421 catccataat cacatcattt aatacagcag acggaatacg tttgttttga ctttcgctaa
    39481 tacgtttaat catatctggt aacttgtgca aacgttgctt tgtctctgca gaaacaaaga
    39541 taattggcgc ataagacaag aattggaagt tatcacgtat atcagcttcc cactgactga
    39601 cagtatgatt gtctttttcg atagtatccc atttattaac tacaataata atacctttac
    39661 cagtttcatg tgcaaaccct gcaatacgct tgtcatactc acgaatacct tcctctgcat
    39721 taatcaccat aagaacaaca tctgagcggt cgatggcgcg catagaacgc ataacagaat
    39781 atttctctgt gttttcatat accttaccag atttacgcat accagcagta tcaatcatag
    39841 tatactcttg cccctggcta tcaacgaaat tagtatcaat agcatcgcgc gtagtaccag
    39901 caactgggct agcaataaca cggtcttccc caaggatggc attaattaaa ctagatttcc
    39961 caacatttgg acgtccaatc aaactaaaac gaataatatc tggattttca ttttcctctt
    40021 ctaccggaag attttccaca atagcatcca atatatcacc agtaccaata ccatgaactg
    40081 atgacaaggg ataaggatcc cctaaaccaa gagagtagaa atcgtaaata tcattgcgca
    40141 tctcaggatt atctacctta ttaacagcta aaataaccgg tttattggtc ttataaagaa
    40201 tacgtgagac atactcatct gcatcagtca caccttcttt acccgacaca acaaacacaa
    40261 taacatcagc ctctgtcata gcaatgtctg cttggtgctt aatttgctcc ataaacggtg
    40321 catcaacatc atcaatccca ccagtatcaa ttaatgaaaa cttacgattt agccactcac
    40381 ccgttgtata gatacgatca cgagtgactc cttcaacgtc ctcaacaatg gaaatacgtt
    40441 ctcctgcaat tctgttaaat aatgttgatt ttccaacatt cggacgacca acaatagcaa
    40501 ctgtaggtaa taccataatt ctcctctcac tattgggaat actcccaatt ccattctatt
    40561 ttattaacgg cgattcggtc cttctaagtg gaattctatc gctaaatacc gtacgcgctc
    40621 cataactcgt tttgcttgcc aagtttcgtc gcttcctttt atattagccc attttctttc
    40681 caaatcgtta aaactataat ttgacgtaaa gaaagtgggc aactcttcta gcatacgatg
    40741 ttgtaatata acttgtaaaa tctcgtcacg aacccaggac gttgcctgtt cagccccaat
    40801 atcatctaaa atcaaaatcg gaacactttt cacagcatca atttcatctt ttacagttcc
    40861 gcttgaaata gcatttttga catcaatagc aaaactaggg aaatgaagaa gagtcgttga
    40921 cacacccttt ctctctgata attccctcgc cattgctgcc attaaatagg atttcccaac
    40981 ccccatatct ccatataaat aaagcccctt gtgattatat gaaggataag tctctacaaa
    41041 atcaagcagt tgtgacatag ctttcattcg actctcgtta ttaatatcaa agtcagtcat
    41101 tctgatattc cgataggact tgggtagatt aactaaatta atacgatcac tgatagcttg
    41161 ttttttttgt gcttcaatta actctcgtgt ttctaaataa gaaacatctg cataaccttc
    41221 attcataacc aaaataggct catagccttt agcaatatat tgactatcct tatttttaaa
    41281 tttctgacgc tcaatcaaaa attgattaaa tttagacatg ctgatattta tttcacgctg
    41341 agaaagttgg tgggttttaa tgaactccgc tacctgagca tctgctaaaa tcatttgaat
    41401 aagctcatca tttgtattac gaggcactct cccttgattt tctaatgctt gtcccactga
    41461 tttcatgatt caccatcctt tcctaatcgt tctaaacgct tgagggcatc tgttttaaac
    41521 tgttctaatt caatttcttt ttctgggcta acctcatctt tataatcagg attactccat
    41581 tcaggaacat tcgattgttt cttcttcgtt tctgttttag tccttacttt ttgatctgaa
    41641 aaatcacgaa ttttaagcac agcatcttcg gccgtcataa cattctgaaa ggcaaaatca
    41701 ttagcaactt tcatgatata ggccttattt aagttagcag attttgtctt attcaaggta
    41761 tataaaacca taacattaat gacttcgtct aggaaattca tttttgccaa atcctcaagt
    41821 aaagtcttct caccacttgt tgtcactgcc ttacggcttc tcttgatttt ttcaaggaac
    41881 actagagcac tatcattttt agattccctt agtatgacct tttcattatt tgtaaaactc
    41941 tgattatctt taatatgctg cccctcattt tgttgaactt tcattcgctg aggcgcaatc
    42001 attccattga tagctgtttg ttttgctaat tgataggtat cataccaatt taaatgataa
    42061 agttctgata cactataaat acctaagaca tcatcttttt cattgttaaa acgtaagcca
    42121 tctcgcatca tcaaacgttt gaaactttcc aagtcaaaaa catttttact ccggtttacc
    42181 tcttgttttg gttgtcctaa atccgagaaa acatctgtaa attttttaga gatatctcta
    42241 gcatttttag gtattttcat atctaattct gccactgcta cctctcctat acgtttttct
    42301 aacaaacgac gatagatggg attagataaa aataactcat ttgacatagc ttgattcaac
    42361 ttaacaaggt aagtacctgt ggcttggtaa acagtcaaca aatccaaagc tgttaacatt
    42421 accaatgcat cttgaaatct tggcatcccg tattgcagat gattgagaac ctctgaaaat
    42481 ttatgacgtc gtattccgtc atcaaaaaaa tgaacaaaat aatcatagag tgccatggca
    42541 tcacttccca taattgggaa atacaattgt attaaactat tactatcata aacaatctta
    42601 ttttgcttaa tatatgtaaa ctcatctatt ggactcatca cacgctacca ctctttttac
    42661 tccgtacgcg ctttgtaatt tgttgaagta gttcttcaat ttcatctaca tctttaaaag
    42721 atttataaac actagcaaag cgcacataag taatctcatc taattcggcg agttcatcca
    42781 tcactaaatt tccgatagtg atactagaaa cctcatcttc atactcactc ctaattttac
    42841 gttcgatacg taagatacaa ttttcaatat cttcgctcga aacagggcgt ttttgagcac
    42901 tctgaataat accattcaaa attttatctc gagaaaattg ctcgcgagta ccatctttct
    42961 ttatgactaa aagaggcaat tcttccaaac gttcaaatgt tgtaaagcgg ttgccacatt
    43021 tttcgcactc tctccggcgt ctgatagtcg taccttcttc agcttgtcgg ctatcaacca
    43081 cactagattt attatagcca cattttggac aacgcatgtc aacaccccta ttctatcata
    43141 tcatttaatc atcttatttt aacacaaatt cagtaataac aaaagaagcg cctctcttat
    43201 cacctcatca tatcaaaaca caaaataatc cttatgtctt ttatcattaa gcataaggat
    43261 ttttcttata tttctttagt ttcttcaaat ttttttatag gaatgcgaac aatgaattca
    43321 gtcccttgat ttagttcact tttaatatcc acctttaaat ggtaagcatc cataatttgt
    43381 ttaaacactg aaagcccgat acctaaacca gcttgtgtac tttctctgtt acgtgattta
    43441 tcagttcgat aaaaacgatc aaagataaac tcaatatctt catctgagat accttctcct
    43501 ttatctttta caactactgt cgcaaaatca ttgtctactg ataaaacaac actgacttct
    43561 ttataactag gagagtactt gatagcgtta tcaattaaga tcatgagagc ttgctcaaaa
    43621 tgtatcttat atatctttcc ccaaacgata tctgaaatat tattttcaaa aataaattga
    43681 aaatcttctc tcaaaatacg gaaattttca ataacaactg atatagagct tgataaatct
    43741 gtcatttcat cttgatgaag ttccaagctt ccttggacgc gaatcatatt taacatgtca
    43801 ttaatcatta aggacatacg atctgcctca tggtatgccg ctgctaaact ttcttcaaga
    43861 atttctggat catctttccc ccaacgttgt aataaaccaa tatgaccctt tacaacagcg
    43921 acaggcgttc ttaattcatg actaacatca ctaatgaatt gtgattgtcg acgagtatag
    43981 tcttccaact gatccaacat cccatcaaaa atcctagaca attctccaat ttcgtcttct
    44041 gaacggacct tacttctaat ttctaaatta ctaggatttt cagatatctg atgcattaag
    44101 tcgtgtaaat ttttaaccgg acgaacaata cgcttagtag cactattaat aacgaccaag
    44161 gctaaaacag tccctaaaac ttccatcata atcaagaaaa caatgatgtt ttgcctcata
    44221 ctataataac gacctaaatt atagaaaatt tgaacgtatc caagcaactg tcctgttttt
    44281 tgagagtaga ttgcttttgt accaacaaaa ccttccatat ctgcaacttt agagatgata
    44341 acttttccca tggatttacc aatagttggc atacccgttc tcagtgtaga aaaaactggt
    44401 ttcctgttga catcgtatag ggtcatttct tgattaacat aaagaatact atcgattgac
    44461 tcgcccgttc taacaattcc attttgacgc ttatgaggat agatgtcatc cgcaaaaacc
    44521 tggttgtaca ggatctctaa aatatcatcg gatgtcaaac tagagttcgc ttgcgacaaa
    44581 cgaactctag ttttttctaa tgacctaccg acattaattt tttccctgtg aaggacgtat
    44641 cttgttgccg aagtataaac taaaacagaa aatactgtaa ataggctaaa aaataagata
    44701 aaaactaatt gcgataattt ttttgacaga ggttgtttta cccctataaa ctgatctttt
    44761 ttatttttca cgaatcacat agcccattcc gcgaacagtt tgaatatatg attcacgacc
    44821 tgggatatca attttacctc ttaggtaacg gatataaaca tcaacaacgt ttgtctctgc
    44881 tgccacatcg tatttccaaa catgttctag caactcttct cgtgtcataa cacgattcat
    44941 atttgtcatc aagacattca acaaatcaaa ttcacgtttt gttagtgaaa tttcctcatc
    45001 accacgcatc gctgaacgat tatgagtatt taatgacaaa tcacggaaac taccactatc
    45061 acctttttct tttgtcttcg tttcaatttc ttggcgtcgg aaaatcgctc taacacgtgc
    45121 taataattct tcgattgcaa acggcttaac aatataatca tctgctccac gatcaagacc
    45181 agctacaata tccataacag aatcacgtgc tgtcatcatc atgatatagg ttgttttttc
    45241 agcctgcagg cgacgtgtga tttcgaaacc atccatctct ggaagcatta aatccagtag
    45301 aatcaaatca aaatcttttt ctaatgctgt gtccaatcct tcacgaccgt ttgtttcaac
    45361 aacaacatca tatccttcat gtagtagttc taacgagacg aagcgagcta aatttttctc
    45421 atcttcgatt attaagatct ttttacccat ctacaccaac cttcttattc tttacttaac
    45481 actatcaatt attttttaga atatctagat aaaaaacaag atagcccaca ttttactaac
    45541 gtttctttaa ataaataaat cgccatccca aacaattgtt cgatagcgag acttaatttt
    45601 tattattgag tagtgtcctc tgtcactttt caattctact aaaaaaaaat taaaatatca
    45661 actgatgtta ttaataattt ttatctaatc actatcaaac ataccttcta aggctgcgaa
    45721 aggattagat ttttcttttt ctttagcttg tttttcttca tactgctttt cagtcattaa
    45781 cgaccaattt ttacctgata aatccgcttc taccccaacc tcatcagctg ccagaacacg
    45841 aagaggtata tttaacaaga tattatcgat aacgctttct tctaaattaa tatcatcttc
    45901 ctctaatatt aagaccaaat cctgatcaac gagctctttt ttagtgctaa cattttcagc
    45961 ttcaataaaa acttcattaa tagtttgctt ttcagataaa acaactggtt tcatagaacg
    46021 actagacggt aatgtaatat cgtaagccat attatagtca agtagataga gaccatcttc
    46081 atacaccaca tgacctgaaa cttgaatatc agaaatatcc attatctctg aatgacgctc
    46141 cataagcgac tctttgatat ctattttttt atcaaaataa agcccctctg gtgatttttt
    46201 gatttctgta agtaacacgt gccatctccc tttatagtat atgtcatatt atatcaagtt
    46261 ttacaggctt tgtcttaaaa aaacaaaacg caaggcatag ctaccctacg ttttgttaac
    46321 gactacatat gacgcaaacg ctcaatacga tctgcaattg gtggatgcgt ataaaataaa
    46381 gactttaacc cttctttttt cgttgggtca ttgatataga gagccgcact agcatcatct
    46441 actggatgcc ccataggttc agaacgatca agtttttcta aagcagagat cattccttgt
    46501 ggatttcttg ttaactcaac agaactagcg tctgctaagt attcgcgctg tcttgaaata
    46561 gctaattgaa ctaaactagc ggctaatggt gcaaggatta gtgataaaat agagaaaatc
    46621 aaaaccaaga tgtttccacc atcttctctg tcatcatcac gcctacgacc tccgccgtaa
    46681 aataacatac ggctaccaat gcttgatatt agagttacag cactcgctaa agctactgca
    46741 atggttgaaa tacggatatc ataattacga atatgactaa cctcatgtcc aataacaccc
    46801 tctagctctt cgcggttcat aacagctaat aggcctgtcg ttgctgcaac agcagcattt
    46861 tctggcttag agcctgtggc aaaggcattt agggagtcat cttcaacaat gaaaacgcgt
    46921 ggcataggta tctgagctat cattgccata tcttcaacaa tatgaaaata atttggcgcc
    46981 tcatcttctg taacttcacg cgcattgttc attgacataa caacattagt tgattgaaaa
    47041 atcatactta ctgcatagat aaccccaata attagagcta gaacaagccc aaattggtag
    47101 ctgcctaata ccaaataacc tacagctgct ccaatagctg ctaaaagaca gaagaagaca
    47161 attaacaaga caactgtttt tctcttatta cttgcaattt gttgatacag caaaagcctc
    47221 tctcccttct caattaaaag tcaaaggaaa cttttggcac ttctttttct tcttcaggag
    47281 tttctaagaa ttggcttggt ttgaacccaa atagttttcc aacaatatta cttgggaaag
    47341 tttctaattt cacattataa ttggatgttg ttgtgttata taactgacgt gaataagaaa
    47401 ttttattttc cgtattagtt agttcttctt gaagtttaac aaagctattg ttggctttta
    47461 aatctgggta attttcagca accgctaaaa tacttgaaag ctgtcttgtt agctcattac
    47521 ttgctgtcat tgcttcagca ggtgtgttag ctttagcaac ttgagcacgc agttctgcaa
    47581 tcttctcaag tgttttccct tcgtacgcag catagccttt aacagtttcg attaggtttg
    47641 gaattaaatc gttacgacgt ttcaattgaa cgtctatttg actccatgat tctttagtat
    47701 gcatgcgact tctaactaaa ctgttataag caacaatcaa ccaaataaca aacaaagcaa
    47761 taattgctat taaaatcata gttcccatag tagtagctcc tttacatttt ttccattata
    47821 gcaattttct ttagattagg aaagtcctgt tattcccatt attagtattt gtgataaaat
    47881 agagtcatta tacgatatag aaattgaggt ttactatgac accccaagca ttttatcaag
    47941 ttctgataga gcatggtatt acacttactg acaaacagaa aaaacaattt gaaacttatt
    48001 ttagacttct tgttgaatgg aatgagaaaa ttaacctaac agctattaca gataaggaag
    48061 aggtttactt aaagcacttc tatgattcta ttgcgccgat tttacaaggt tatattgaca
    48121 atagtcctct tagcatttta gatattggtg ctggtgcagg ttttcctagt attcctatga
    48181 aaatccttta tcctgaaatt gatattacta ttattgattc actgaataaa cgcattaatt
    48241 ttctaaacat attagctaat gaacttgaac tttcaggagt tcacttcttc catgggcgcg
    48301 ctgaggactt tggtcaagat aaagtatttc gagctaaatt tgatatcgta acagctcgtg
    48361 cagtagctag aatgcaggtt ctagcagaat tgacaatacc ttttcttaaa gttaatggtc
    48421 gcttgattgc actgaaagct gcagctgctg aagaggaatt aatttctgca gaaaaagctt
    48481 tgaaaacact attttcacaa gtaactgtaa ataaaaatta taagttacct aatggtgatg
    48541 accgaaatat cacaatcgtt agcaagaaaa aagaaacgcc taataaatac cctcgtaaag
    48601 ctggtactcc aaacaagaag cctttgtaaa gttgggagct agtatgaaac atttctttga
    48661 ttacaagaca atgtctgtcg cacgtaaact cagcattagt tttatcgctg ttatcctatt
    48721 gggaagtatt ttactttcac ttccaatttt tcaatatgct aacgccccta aaacacatta
    48781 cattgatcac cttttcacga ctgtttcaat ggtatgtgta actggtttat ctgtttttcc
    48841 tatcagtaag gtttataacg gttgggggca gattgtagct attttactca tgcaaacagg
    48901 gggacttggt ttagtaacac taatgtcgtt gtcttactat accctaagac gtaaaatgtc
    48961 gttaaatgat cagacccttt tgcagtcagc gataacttat aacagttcga cagatttaaa
    49021 aaaatatctc tatatgattt ttaaagtaac tttaacttta gaagttttag cagccagcat
    49081 tttagccata gattttattc ctcgatttgg tttgggtcat ggtatcttta atagtatatt
    49141 cttagctgtc tcagcctttt gtaatgccgg ttttgataat ctaggggcaa cgagcttagc
    49201 tcaatttaaa ctaaatcctt tggttaacat tatcgtttgt ttcttgatta tttcaggagg
    49261 acttggattt gccgtatgga aagatttaat tgaagctact atacaaacat ctcacaaggg
    49321 accaaaatta atcaagacat ttccaaaacg cctcagcaac cattctaaac tggttttaaa
    49381 aacaacaacc attattttac taacaggaac tttgctttct tggttgttag agtttggaaa
    49441 ttttagaaca attgctaatt tatctctacc taaacaacta atggtcagct ttttccaaac
    49501 agttacgatg cgtaccgctg gattttcaac tattgattat acccaaactg atttcgcaac
    49561 caaccttgtt tatattatcc aaatgttaat tggaggagct cctggtggaa cggcaggagg
    49621 atttaaagtc actgttatcg ccattttact cttactattt aaagccgaac tgagtggcca
    49681 aagccaagtg acttttcatt atcgaacaat tccttccagt attattaaac aaacactatc
    49741 gattttaact ttctttttta tcatacttat tagcggctat ctccttcttc tggaactcaa
    49801 tccccatata gatccttttt ctcttttctt tgaggcatct agtgccttag caaccgtggg
    49861 tgtaacaatg aacactacca atcaattaac tctgggcggt cgaattgtaa ttatgttcct
    49921 catgtttatt ggtcgagtcg gtccaattac cgtactgcta tcaattcttc agaaaaaaga
    49981 gaaggaaatt cactatgctg aaacagaaat tattctaggt tagaaaaggt cttttatgaa
    50041 aacaaaaatt attggtgtac ttggtctagg aatatttgga caaacactcg cacaagaact
    50101 aagtaacttt gaacaagatg ttattgctat tgacagcaat cctgaaaatg tacaagctgt
    50161 cgccgaagtt gttacaaaag cagctatcgg agacattact gatttagctt tcctaaaaca
    50221 catcgggatc agtgactgtg atactgttat tattgctaca ggaaacagtt tagagagctc
    50281 agtattggcc gtaatgcact gtaaaaagtt aggcgtccca caagttattg ctaaagctcg
    50341 aaaccttgta tacgaagaag tactttatga aattggtgct gatttggtta tctctccgga
    50401 gcgagaatct gggcaaaatg ttgctgcaaa cctcatgaga aataaaatta cagatgtctt
    50461 ccagattgaa tctgatattt ctgtcattga atttaaaatt cccaaatcat gggtagggaa
    50521 aactgtcgaa caattaaata tccgtcacaa gtttgatctt aacctcatag gtatcagaaa
    50581 agcaaaaaac aaacctgtcg ataccgaagt tccaatcaat agtccacttg aagaaggtat
    50641 tattctagtg gctatcgcta actcagatgc cttccaacgc tatgactatt taggatactt
    50701 taactaaaga aaataaaaaa ctgactatta aggtcagttt tttattgcca tgggttatag
    50761 aaacgtccat gattaaggtg aattaaataa aggcttacaa acagactagc tagagcaagt
    50821 atgattaaga caatgtcact tttttccaaa gattgcttag agtaccaagt acgtctttta
    50881 tttttaccaa atcgtcgaag ttccatagca gttgctactg tatcaatacg ttctagagag
    50941 ctaaaaatta atggtgtgac aatctgcaaa ttccccttaa tgcgtgctac taagttcgat
    51001 ttttttgata attctattcc tcttgcctct tgtgctcgac gtattgtata aaattcttct
    51061 tgaacatcag gtatatatct aagggtcaaa ctaacagcat aagctatttt atatgataaa
    51121 cctaattgat ttaaacttga tgcgaactga ctaggattag ttgtcatcaa aaacaaaaga
    51181 gctaaaggca ctgtacaaaa gtattttaaa atcagattaa agaggtaaaa caactcttgg
    51241 ctagtcacat catatgggcc aattcctttt aacaataaag aagaactacc ataaatctta
    51301 tcaccataat ggggagcaaa aagatagacc attataatat ttaaaacagc gaatacaaca
    51361 actaaagtca taacaaagga cacctgttgc catttgattt ttgctaatct aaataagact
    51421 agcgatccca atgaaacaac aattaaaaat cttgtatcat aagtggtcat gcaagcaaca
    51481 gaaactaaaa taaaaaatag taacttactg gcacctgata gcctgtatag aaagccataa
    51541 ccctcttgat aaccaattaa ttgtttagcc attcttacct ccttgattat caatgtacca
    51601 ttgtgttagt ttctgtggag atatccctaa tctgtctgct aaagcaaata aactcgtttt
    51661 ctttaaatga gtctttttca aaagctctgt atttgaaagg atgttaatag gatgatcatc
    51721 tgcaacaatc agaccattgt cgattacaat tgcacgatct gtatactcta acataagctg
    51781 catatcatgt gtaatcataa caatggtatg tccatcacaa cttagtttat caaggaaaga
    51841 catcatttca gtataatgct tcatatcttg accagctgtt ggttcatcca aaataataac
    51901 ttctggattt aaaatcagta tcgaagcaat cgtaacacgt tttttctgac cgaatgatag
    51961 agccgatata ggccaattcc taaattggta gagcccacaa acctttaata tatcgtagac
    52021 acgactttct atatcattat cactaaaacc tcttaaacgg agacccagag caacttcatc
    52081 aaaaaccatc gcttgactta tcatctgatt gggattttgc aaaacatagc caattcgttc
    52141 tgctctttct tttatagagt cttgactgac atcttcatta ttataaatta gagtagcatt
    52201 attagggata aattgacata acgctttagc taaagtagat ttacctgcac catttttccc
    52261 aacgatagct aaccgctgac ctttatataa atcaaacgaa acatcatcta aagtattatt
    52321 ctctaaatca tatgacaccg acaaatgctg tactgataat aatttatctg tatgtttatc
    52381 taaatctaat gctcgcattt taggtatcaa tagttcactg aaatcaaagt catcaataga
    52441 cgtcattgta ttttgttttt caaagtcata tcctaaattt ttgagagcag aaatatacag
    52501 tggttctcga ataccatatt cagataaaaa attagttttt aaaagttgat ccggctcccc
    52561 attataaata atttgtccat cactaaaaag aactacacga tctactcgct cataaagaac
    52621 atcttccaat cgatgttcaa taataatagt ggtcgcatct tgctcttgat gaatacggtc
    52681 aaccaatgcc atgatatctt gtcccgattg tggatctaga ttagccaaag gctcatcaaa
    52741 taatagaatt gggctatcgt ctactaaaac tccagctaaa ctaacccgtt gtttttgtcc
    52801 tcccgacaag tcttgtggac gtttagacaa aagaggagct atttctaaac gttttgccca
    52861 catttcaacg atagaggcca tttcttcctg tgctacaaca tcattttcta atgcaaaagc
    52921 aatatcttct gctactgtca atcctataaa ttggccatct ggatcttgta aaaccgtaga
    52981 aactaaatga gacttgtcat atattgacaa gtcaaaagca tttttatggt taatcgtaaa
    53041 aataccacta tattggcctt tatgagtatt tgggataata ccatttaaac aatgaccaag
    53101 cgttgacttc cccgaccctg aaggtcctaa tatcaagacc ttttcacctt taggaatgct
    53161 taagttaatc ccttttaaag ttggttcaga ttgaacgtca tattgaaagg taaaatcctt
    53221 ccattcaata aaatctttca tagattactc tcttatccta atctttggat aaactacctt
    53281 tttgtggtcg tgatttagcg tatgctagca acaaaagcgt accacctact ccaatagtaa
    53341 tggaattcac aaggcttgat aagaaacctt gtgcaaaaac tttgcttgca ggttcgctat
    53401 aaaagaaaat atcaccatac ggtgcaacga cagaccaacc tataaggtta gcaataactt
    53461 gaactacatt aaatagaagc aaatccttct tagtcatgcc agataattga atagctaatt
    53521 ttttagcaaa aaagcctatc atcaaaccta aaagaccact aactaaaacc caagtccacc
    53581 acggagaacc atattgaatc gaatctttca atgcgtgtcc gataaaacca gtaaaaaaac
    53641 caactcctgg tccataaata actgcaaata atgctaaaac tgcatactgc aattgaatat
    53701 tagtatttgg aattggtgtt ggtatattaa ctagcatacc tataatgata aaaagtgcag
    53761 ctccaattcc agtcgctaca acctttttta ttgtgtttgt attcataaaa acctctctga
    53821 aaaacaagct taaacagtca cttacaatct gttttatttt atcatatttc aagctgattt
    53881 gaaaccttat tgacaagaag aactgaaaaa cgattgaaaa taatattaaa taccaaaaaa
    53941 gatctcagat agaataccta agaccttcat tagtttattt ttttccaaaa actgattttt
    54001 cagctacagc cgtgaacaga acgtctgtcg atgaatttaa ggctgtctca cacgaatcct
    54061 gtatcacccc tacaatgaaa ccaactccta cgacttgcat tgcaacgtca tttgaaatac
    54121 caaataagct acatgccacc ggaatcaata aaagtgaacc accagttaca ccagaagctc
    54181 cacatgctga tacagcagca accacactta ataagaatgc tgttggaaaa tcaacagtaa
    54241 ttcctaaagt atttactgct gctaaagtca ggatattaat tgtaatagct gcaccagcca
    54301 tattgatagc tgcaccaagc gggatagata ctgagtaagt atctttatct aatcctaaat
    54361 cctcacatag tctcatatta acaggaatat tcgccgcaga actacgtgta aagaaagctg
    54421 taatacctga atcctttaag catcgtaata ccaaagggta aggattttta cgcatcataa
    54481 caaaagcaat gattgggtta acgacaagag ctacaaataa catcgtaccc accaaaacta
    54541 atattaaaag accataatca cctagtatac caataccgtt ttctgaaatg ctagtgaaaa
    54601 ccaaccccat aataccaatt ggggccaaat tgataatcca tttgacaact tgtgaagtag
    54661 catcagcaaa agtttgcatt aaatcttttg tacgctgatt actgcttcgc atcgccaaac
    54721 caaaaataac cgcccaaaat agaatactca tataattggc ctcggtaatg gcatgaatgg
    54781 gattatcaac aattttcaac aaaagagatt gaaagacttc tgaaatcccc ttgggaggta
    54841 ataagtctgt ctttgctggc gttttcaata tcagtgtcaa agggaaaata tagttcacaa
    54901 caacagcgat tagggccgct gcaaaagttg cagtgatata caaaccaatt atcgttgaca
    54961 tattggtctt accgccctca cggtgttgcg acaaggcact aataactaaa gtgaaaacta
    55021 aaagaggggc aatagctttc aaccctccta caaaaagttg accaagtaaa ccaatcgcag
    55081 aggctttagg gactaaaata cccaagaata agccaataac gactccaatt gttatttttt
    55141 ttatcaagct tgtttttctc caagctttta aaaatcgttt catactaaac tcctaaaaaa
    55201 ttcttaaact ctatgatatg ataaaaaaaa agattcgtca aatacctttt ttatttttta
    55261 gaggttgacg aattctaaat ttttctgata taattacttt ttgaaatatt caatcaattt
    55321 cgaaaataaa atattttttt gtacagaaag gtacattgat gaaactaaaa aacacttata
    55381 tcattatcgg cttgatgctt tttgcgctgt tctttggagc cggtaatctt atttatccag
    55441 cctacttagg tatctactct ggttctaatt gggcgtgggc tatcattggg ttctgcttaa
    55501 caggcgtaac tttaccccta cttggtgttg cagctgtggc gtactcagga gcaaccgatg
    55561 ttgaaagctt caccaaacct gtctctaaaa catatgctat tttctttgca gtagcactat
    55621 acctatctat tggacctttc tttgccattc ctcgaacagg ggcaacctca ttctcagttg
    55681 gtattcaacc tatcttaggc gacagctcct tagtcaaaat tatctatggt ttaattttct
    55741 ttggtatttc ttatttctta gctattagac ctagtcagat tgtagatcgt attggtaaat
    55801 atctaactcc ctttttacta actgttatta gtattttaat tattgcctca ctcatccatc
    55861 cagctgctat cgttggaaca ccacataacg cttcaacgaa tgtcagcgat gctttcaagg
    55921 atttaccatt cctagcagga ctgattcagg ggtatggtac aatggatgcc cttgcatcaa
    55981 ttgcttttgc tattatcgtt attcaagcaa gtaaacagta cggagcaatt accaaaaaag
    56041 aaattacttc tatggcactt aaatctggtg ctatcgctac atttctttta gcttttattt
    56101 atatttttgt aggacgtatt ggtgcaactt cacaaagctt gtttaaattt gcaaatggtt
    56161 ccttcttact tcacaatact ccaatagacg gtgggcatgt tcttagtcaa tcagctaact
    56221 tctatttagg aattgtggga caagctattt taggtacggc tatttttctt gcttgcttaa
    56281 caacagctac tggattaatc actgcttgtg cggaatactt ccacaaatta cttccaaaga
    56341 ttagtcacat tacttgggct acaatcttta ctttaattgc tattacgttc tattttggtg
    56401 gtttatcaga gattatccgt tggtcactac cagttctcta ccttctctat ccattaacta
    56461 tagtcttaat tttcttagtc ttttttgatc aaaaatttga aagtagccgc attgtctacc
    56521 aaacttctat tgccgccact gctgtcgctg ctttatacga tgccttatca aaactcggcg
    56581 aaatgactgg tttatttacc attccatccg ctttgaccac attctttact aaagttgttc
    56641 ctttaggtga atattctatg ggatggatta gttttgcaat ttgtggtgtc ttagttggtc
    56701 ttattctaaa aaaagtaaaa gcataataat ttaaagcagt acgaaaatta ctgcttttat
    56761 tttaaaataa ttattgtaac cgctttcttt tggtggtata atagccttga aaataaataa
    56821 tttttggagg aactctaata tgagagctgc aacttatctt tcagctaatg aattatcact
    56881 tactgataag gctaaacctc aagtaataaa acccacagat gcggttgtaa gacttgtcaa
    56941 aacaaccatt tgtggtactg atctacatat ccttggtggc gatgttcctg cttgtaaacc
    57001 aggaacaact ttaggtcatg aaggcattgg tatcgtcgaa gaagttggtt ctgctgtcaa
    57061 tcattttaaa gtcggagata aagtaattat ttcttgtgtc actgcctgta atacttgcta
    57121 ttactgtaag caccagttgc cttcacattg tgaagatggc gggtggattt taggacatct
    57181 tattgaaggt actcaagcag aatatgttca tattccacat gcagatggta gcctttatca
    57241 tgcacctgaa ggtgtttgtg acgatgcatt ggttatgtta tctgatattt taccaacttc
    57301 atacgaaatc ggcgtcttac cttctcacat taaaccaggt gatactgttt gtattgtagg
    57361 cgctggacca attggactat ctgccttact aactgcacaa ttctattctc ctgccaaaat
    57421 tattatggtt gatttatctc aaaaacgact tgaagcctct aaaaaatttg gtgcaacaca
    57481 caccattcta tcaacgtcta ctcaagaagt taaagaagaa attgataaaa ttaccaaagg
    57541 ccgtggcgtt gatgtcgtat tagaatgtgt tggttatcct gcaacatttg atatttgtca
    57601 aaatgtagtt tctattggag gacatatcgc aaatgttggt gttcatggta aacctgttga
    57661 atttaacctc caagatttat ggataaaaaa tataaccctt aatactggat tagttaatgc
    57721 taatacaact gaaatgctcc ttgaagttct tgagacaggc aaaatcgatg caacacaact
    57781 agtaacacat cacttcaagc tatctgaaat cgaagaagct tataaggtat ttaaagctgc
    57841 tgaagaaaac aatactctca aagttatcat cgaaaacgat attactccag cctgataatt
    57901 tttaaacaaa tgaaaatgtg agaagaatag ccttctcaca tttttattta tgactcaagc
    57961 gacgtgttaa aaaatcacct aaaaattgga taaagaaaat taataataaa atcagaatag
    58021 tcgctactaa agtaatatca tcacgagaat agttatatcc tttagtaata gcaacagaac
    58081 ccaatcctcc tgcaccaata gcgccagcca tagctgtttc acctactaaa gaaatcaaag
    58141 taaccgttga tactcgaatt aaatctggta gaccttcacg aagataaact acaataatat
    58201 cccaaagtgt tccacctgag gcttgtgcag cctcaataac tccaccatca agttcagcta
    58261 aaacaacttg aacttgacga gcaaaaaatg ggaaaactgc caaagaaaga ggtaccaaag
    58321 ctgctggtga accaagtgtt gttcctacaa ttacgcgagt tactggcgca atcaaagcaa
    58381 gaagaataat gaagggcaga gctctaaaaa cagaaacaac tttatctaaa actccaaata
    58441 ctaatttatt agcaataact cctctaggac tagttaaaac aaggaataaa cctcctaaca
    58501 atcccattaa acctccaata aggaacgacc aaaaagtcat ataaagggtt tgtacaatag
    58561 ctgtctgcca gccgtaagca ccttcccaac ccatttgata tacatttggt aaatgtgttt
    58621 gaatccactc tatcatgctt taccaccttc tttcaataac gttaattgaa cttgcgaatc
    58681 tgtaattgct tggcaggctt gtcttaattt ttcttcgtca cctgataaga taactaccat
    58741 ttctccaact ggaataccat ctaaaatttc aatattccca tagaggatgt tagcagttac
    58801 ttcaaattct ttataaattt gatttaaaag cggctcatcc gtgctatgcc cagcgtattt
    58861 cagttgaact aatttgctac ctactggtag atttttgaca acttcttgtt gctcaatctt
    58921 aagcattgcc tcatcaatcc cagtagctat ttttataaaa tcttgtgtaa gactctctct
    58981 aggatgtgaa aaaatgtcta aaactgatcc ttcttcaatc aatttaccat tttgcataac
    59041 agcaacgcga ttagcaatgt ctttgacaat ttgcatctca tgtgtaatca aaacaatcgt
    59101 aagtcccaat tttttattca gatcttggag aagggcaagt atttgttttg ttgttttagg
    59161 gtcaagtgca gaggtagact catctgaaat taagatttta ggatcgttag ccaatgcacg
    59221 cgcaatggca acacgctgtt tttgccctcc tgataattgt gaaggatagt tttgagcacg
    59281 atcggataat ccaacaagtt ctaataattt agcaacctta gctgctttcg cctcttttga
    59341 taaacctgaa tgtttaagag caaaaactac attctgctct gctgtcaact gtgacattag
    59401 gttaaaatgt tgaaaaatca ttccaatttc acggcgctgt tcgcgtaatt gtgtgctagt
    59461 cagtgtcacc ttgttgtcgt aaataacttg gtcatcaata gtaattttac ctgctgaagg
    59521 ttcttgtagc aaattaataa cacgaacaag ggtagatttt ccggctcctg aataaccgac
    59581 aattccatat atatcacctt gattaatatg gatagtaaca tctttaacag cattaatctc
    59641 acgtttcttt tggtggaaag taacatcaat gttgtctaat ttaataattg gttctttact
    59701 catatgattc tattaaagcc tccactaatt caatatgggt gtaataatca gcaatactaa
    59761 cattttcatc aacgccatgg tctcgactat tggcatgtcc tatgccaata gctgcaattg
    59821 gcgctcttag agcttgatga actaaataca ttggtcctgt acctggtgag gtaggtaaca
    59881 gagatatccc ttctggataa aattgctcag ctaaatcaac aacctttagg atagatggcg
    59941 ctgacatatc actacgataa gacatttcac caagggtata ggttagctca atgtctttaa
    60001 agccattctc tttcaaatga ttttgtattg aatctagtac acctttcggg gttaaccctg
    60061 gtactaacct aacctctgct ttacatttag cataagcagg taaaattgtt ttgacgcctt
    60121 caccttgata acctgaggtt ataccttcaa tggctataga aggttcaaaa taaagctttc
    60181 tcaaaaaagt tttatgacta tctgctaaac tgggcaaaac taattggtaa gcaccttcta
    60241 gcgcctttgc agatctataa gaatattttt caactaattc taactctctc ttagtagggg
    60301 gaataacttt atcataaata ccttctacca atatatgacc tttgttatcc cttaacgtat
    60361 tgagggcttg aattaaatac catgtcgagg aatcaacaac cccaccaaag gatgaatgaa
    60421 tatcaacatc cgctgaactt actgataaat caaaagtgac aatgccttta tttccaccag
    60481 caatttccaa ctgtccttta ggatttttag ggccatcttc ccacacgatc aaatcagccc
    60541 cttgtagctg ctcttgatac ttttct
//
LOCUS       DATRNK010000006        48783 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.006, whole genome shotgun sequence.
ACCESSION   DATRNK010000006 DATRNK010000000
VERSION     DATRNK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48783
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.006"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            39..1268
                     /gene="secY2"
                     /locus_tag="VBC52_000365"
     CDS             39..1268
                     /gene="secY2"
                     /locus_tag="VBC52_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEO6881972.1"
                     /translation="MKLLYIFEKNIILRKILITFSLIIIFLLGRYVPIPGVLISAYKG
                     QDNNFATLYSTVTGGNLSQVGVFSLGIGPMMTTMILLRLFTIGKYSSGVSQKVQQFRQ
                     NVVMLVIAIIQGLAIAISFQYHNGFSLTKLLLATMILVTGAYIISWIGNLNAEYGFGG
                     MTILVVVGMLVGQFNNIPLIFELFQDGYQLAIILFLLWTLVAMYLMITFERSEYRIPV
                     MRTSIHNRLVDDAYMPIKVNASGGMAFMYVYTLLMFPQYIIILLRSIFPTNPDITSYN
                     DYFSLSSIQGVVIYMILMLVLSVAFTFVNIDPTKISEAMRESGDFIPNYRPGKETQSY
                     LSKICYLFGTFSGFFMAFLGGVPLLFALGNDNLRTVSSMTGIFMMITGMSFMILDEFQ
                     VIRIRKQYTSVFENEEN"
     gene            1268..2812
                     /gene="asp1"
                     /locus_tag="VBC52_000366"
     CDS             1268..2812
                     /gene="asp1"
                     /locus_tag="VBC52_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEO6881973.1"
                     /translation="MFHFIPSWYNENRTWYDNNYLWYFKPTNVGFDDTINQMKMFDYA
                     GKESRLVVLNYMPNLRYYLHRYDLLESGYYSVFDDIQEIGNVRQQMIDFRQLNWPEGV
                     DFTYTPFIVLVKKSGDLIAKVQFGEEGNLTHIDYFANEQIAKKYLFDDRGFLSSILYY
                     DNGGEAYQDYLASSGERIMREYLREGDHHVEINPKKAIHFLKLSYSDIEELIREKYLT
                     YLHKEVSKSDTIIASFNQVHNAFIVGNTSKGNLILSVFSERNNAHNVLEDYSSLSRAD
                     AIICDRLDIAAQLKEKIDKPVVHVSPFDTRLALGKSNQVRDLEIYFVIDRLSHKELQK
                     SLTSLYKVMLKNNDIKVTFVSYEREFESRQLTYDYLKEATKVFDQKFFSLSDDDANQV
                     LMQSDEVEKEKTRLSFTHPLSETDIINRLEYVRLIIDISKIPDLYTQIAGISSGIPQI
                     NTILTEFVEHRKNGYVIEEIQELEKAIPYYCEQLTNWNRSLIYSVDKINDYTGGQLVE
                     RIINSY"
     gene            2819..4378
                     /gene="asp2"
                     /locus_tag="VBC52_000367"
     CDS             2819..4378
                     /gene="asp2"
                     /locus_tag="VBC52_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEO6881974.1"
                     /translation="MSKIKLTILQVGEENWATKENIPNDMEWLFIKPDQISDFVTTEN
                     NYLTSSKLLQKLPRKISALLLTEQTYGPELSSLSSFFEVYEVFYPKDKHATGITEEFL
                     RSKMAQRYDSSSPAQLIRQFYKGLFIGQYGEKLQVSQIQIRNDFEGVVNYQGNNYLEL
                     EGQFGEDYSFLLNFAYNIPFSSDFYNELFLEHIIEGDIDIRLVISLIVDGSVDDIAKE
                     WYFEKEDLNQLISLESDISGSLAVKLFAKGKGIIKLGPLHRRNGRGGLGTFLLGGERH
                     IDAIGHEFMTYFDPVDFKPPLTVYFSGFRSAEGFEGFWMMKSMKTPFMLICDPRLQGG
                     AFYIGSKEYEQKIVDAIQEKLAFLNFSSDQLILSGLSMGTYGATYHGAKLNPHAIIIG
                     KPIFNLGTVAQRERLERPDGFATSLDIQLLNQGDLTSSSSEKLNNLFWKSIEEGDFSN
                     TTFALAYMKNDDYDATAFSDLLQYFRGKKHKILGRGWDGRHGDCSAEVGAWFTSQYRR
                     MLSNDFGRKEE"
     gene            4375..5367
                     /gene="asp3"
                     /locus_tag="VBC52_000368"
     CDS             4375..5367
                     /gene="asp3"
                     /locus_tag="VBC52_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEO6881975.1"
                     /translation="MSTISYIYWDDFSRFSYNFGTKLQFLGKSVCFENPLAPSSTNLY
                     TWSSQTNYQSKRISPNLPLLRKGTRYSLSLNAELDLVGSLFVRIEFYNRFNESIGFEL
                     LKKDSIIFIYPKEAYTYTISLINAGCSDFTFHYLKLEEVTDSVIQEKNLSTEFTIEEH
                     QDVFNLLLVEKKDSVYINKIESISQLQQKVELVSNPSLNSDSLILPELEKGLEDALKV
                     FPNIKINVIAYGTQGNFAALYYAKKFPRITAYINDCFAPFGILLKSLPHLTAKQQIFL
                     REVWDTRETSPNVKHYGLVSENSSLNLVSMILSGNEHLPYLTLLKKQDDNYDSL"
     gene            5354..7741
                     /gene="secA2"
                     /locus_tag="VBC52_000369"
     CDS             5354..7741
                     /gene="secA2"
                     /locus_tag="VBC52_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEO6881976.1"
                     /translation="MTAFNSLFSLDKKRLKKLQRTLNTINSLKDQMATLSNEELQAKT
                     TEFRKRLVNGETLDDICAEAFAVVREADKRVLGLFPYDVQVIGGLVLHQGNTAEMKTG
                     EGKTLTATMPLYLNALEGKGAMLLTNNSYLAIRDAEEMGKVYRFLGLSVGVGVSDNEE
                     EDRDAATKRAVYSSDIVYSTSSALGFDYLIDNLASSKSQKYMPKLHYAIVDEADAVLL
                     DMAQTPLVISGSPRVQSNLYKIADELILSFEEQVDYYFDKERQEVWIKNQGVREAERY
                     FRIPHFYKQSNRELVRHLNLSLKAHKLFERGKDYVVDDGEIKLLDATNGRVLEGTKLQ
                     GGVHQAIEQKEHLNVTPESRAMASITYQNLFRMFTKLAGMTGTGKTAEKEFIEVYDME
                     VVRIPTNSPVRRIDYPDKIYTTLPEKIYATIEFVKQVHDTGQPILLVAGSVRMSELFS
                     ELLLLSGIPHSLLNAQSAVKEAQMIAEAGQKGAVTVATNMAGRGTDIKLGKGVSELGG
                     LAVIGTERMKSQRMDLQLRGRSGRQGDIGFSQFFVSFEDDLMIESGPKWAQAYFRKNR
                     DKVNPEKPKALGQRRFQKLFQQTQEASDGKGESARSQTIEFDSSVQLQREYVYRERNA
                     LINGESGHFSPRQIIDTVISSFIAYLDGEVEKEELIFEVTRFIFDNMSYNLQGISKEM
                     SLEEIKNYLFKIADEILREKHNLLGDSFGDFERTAALKAIDEAWIEEVDYLQQLRTVA
                     TARQTAQRNPVFEYHKEAYKSYNIMKKEIREQTFRNLLLSEVSFNENGDLQIYFI"
     gene            7755..9263
                     /gene="gtfA"
                     /locus_tag="VBC52_000370"
     CDS             7755..9263
                     /gene="gtfA"
                     /locus_tag="VBC52_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEO6881977.1"
                     /translation="MTVYNINHGIGWASSGVEYAQAYRGSVLRKLGIDAKFIFTDFFS
                     ADNIISLTRNIGFQDKEIIWLYNFFSDIELAPTTFSIEDLQKQYLGKLVRKEDRGRVI
                     KFFYEDENIYLTVYLDNFNKDKVHRVEIVSNNNLIRKDYYSYTRTFSEYYYPKDGVAH
                     LYQRRFYNEDESTAFLEMVENSSSRFIINGRLLPSKVAFFDYFLESMTFTSKDIILLD
                     RGTDTAQSLLRHGKPAKLGTVVHAEHFSENAVTADTILWNNYYDYQFTNANRFDFFIT
                     STDKQTELLEQQFKQFTNHNPRIITIPVGSIDNLKMPMDNRRPYSILTASRLASEKHV
                     DWLVRAVIRIREILPEVTFDIYGSGGEEEKIRNIINAANTTEYIRLMGHRNLSNVYQN
                     YELYLTASKSEGFGLTLLEAIGAGLPLIGFDVRYGNQTFIKDGENGYLIPRFDMDDEE
                     AIVEAFKEKVLQLFQQDQKALREASYAIAEGFLTSEVEGKWYNLVKELVQDD"
     gene            9256..10581
                     /gene="gtfB"
                     /locus_tag="VBC52_000371"
     CDS             9256..10581
                     /gene="gtfB"
                     /locus_tag="VBC52_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEO6881978.1"
                     /translation="MINLFDSYTQSSWDLHFSLIKSGYINPTIALNDDGFLPDDVTSP
                     YLYYTGFAKIGAGRPLYYNELRVPDTWEIIGFSSGADIVDLGVKKGRIIYANPNHRRL
                     IKEVDWFDEQGRVILKDRFNKFGFCFAQTFYNVDGQAIQTSYYNKDGQEVISENHMTG
                     DYILNDNNQFKVFKSKVEFVINYLQEAKFNLDRIFYNSLSTPFLVSFYLNRLESKDVL
                     FWQEPLVDDIPGNMRLLLNNPSPNTKIVIQSYEAYANAMRLLTDEEQKQVSFLGFMYP
                     LKETEKLHNQALILTNSDQIEALESLVTSLPNLTFNIGALTEMSSELMNFGKYDNVVL
                     YPNITTNQIQYLSNICAFYLDINHHNEILSAVRSAFEHQQLIFAFEETSHQIRFVSPK
                     NIFPKKDIFTFISHLQPLIGNKCNIEKALKQQLEDCHVSSSTQYQSVIN"
     gene            10592..10780
                     /locus_tag="VBC52_000372"
     CDS             10592..10780
                     /locus_tag="VBC52_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881979.1"
                     /translation="MKKKNLLQESINKRLSEERYQIPKEKREKKRFDMQLILIISILI
                     GLIMSIIGIIRFFLTYSS"
     gene            10864..11328
                     /locus_tag="VBC52_000373"
     CDS             10864..11328
                     /locus_tag="VBC52_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO6881980.1"
                     /translation="MSRSQKVILTNMCLIEDNKGRIVMQIRDPKRYSWSGAALPGGHI
                     EEGESLYDALVREVREETGLTISNPKLVGMKHWYTDEGIRYLVFLYKTYDFSGELQSS
                     DEGDVIWIERSQLDKIELAYDMLNLLRVFDEDDISEMFYSKRLEDGFIRKFY"
     gene            complement(11373..12845)
                     /locus_tag="VBC52_000374"
     CDS             complement(11373..12845)
                     /locus_tag="VBC52_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6881981.1"
                     /translation="MEKTKSFFGHPRGLSTLFFTEMWERFSYYGMRAILLYYMYYSVS
                     QGGLGMDKTVAASIMAVYGSLVYLSSVIGGFVSDRILGSRKTVLYGGILIMLGHIALA
                     TPFGQTALFISIALIILGTGLLKPNVSEMVGNLYGENDSRRDAGFSIFVFGINLGAFI
                     SPIVVGYLGQEVNFHLGFSLAAIGMFFGLLQYTLDGKKYLTEESLRPNDPLSPEEKSS
                     LYKKVGLILIGIVIVLILLHLMHMLTIEVIIDIFSIIAIAIPIIYFIKILSSKKISSV
                     ERSRVWAYIPLFIASILFWSIEEQGSVVLALFADEQTKLYLNFFGHHINFPSSYFQSM
                     NPLFIMLYVPFFAWLWAKWGSKQPSSPKKFAYGLFFAGASFLWMMLPGLLFGVNAKVS
                     PLWLTMSWAIVIVGEMLISPVGLSATSKLAPKAFQAQMMSIWFLSNAAAQAINAQIVK
                     LYTPDTQILYYGVVGGITVVFGFILLFYVPRIEKLMSGVK"
     gene            complement(13076..13912)
                     /locus_tag="VBC52_000375"
     CDS             complement(13076..13912)
                     /locus_tag="VBC52_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6881982.1"
                     /translation="MNKKKRLNLTFVYILLIVLSIMWLFPIVWVVLTSFRGEGSAFVN
                     YFIPKTWTLDNYAKLFTQNTFPFGQWFLNTLFVATCTCILSTLITVAMAYSLSRIKFK
                     HRNGFLKLALVLNMFPGFMSMIAVYYILKALNLDQTLTALIFVYSAGAALTFYIAKGF
                     FDTIPYSLDESAMIDGATRLDIFLKITLPLSKPIIVYTALIAFMGPWMDFIFAKVILG
                     DATSKYTVAIGLFSMLQQDTINQWFMSFTAGSVIIAIPITILFMFMQKYYVEGITGGS
                     VK"
     gene            complement(13912..15282)
                     /locus_tag="VBC52_000376"
     CDS             complement(13912..15282)
                     /locus_tag="VBC52_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6881983.1"
                     /translation="MNKKLISLDGMSLKEVFQKGDLATKLSFLIMGLANLKNKQIVKG
                     LLFLISEILFLITFVYQVIPAVKGLISLGTQEQGMTTKTVDGIKIQVATQGDNSMLML
                     IFGLASLIFCCVFAYIYWSNIKSAAHLLTLKEEGREIPSFKKDIKSLTDGRFHMTLMS
                     IPLIGVLLFTILPLVYMICLAFTNYDHNHLPPKSLFDWVGFANFGNIFSGRMASTFFP
                     VLSWTLIWAVFATVTNFFFGIILALLINTKGLKFKKMWRTIFVITMAVPQFISLLIMR
                     NLLSDAGPVNALLIKWGLISSAHPLPFLSDPVWAKFSIIFVNMWVGIPVTMLVATGII
                     MNLPAEQIEAAEIDGANKFQVFQSITFPQILLIMTPTLIQQFIGNINNFNVIYLLTQG
                     GPTNSTYYQAGSTDLLVTWLYNLTVTAADYNLASVVGILIFILSAVFSLLAYTRTNSY
                     KEGAAK"
     gene            complement(15380..16627)
                     /locus_tag="VBC52_000377"
     CDS             complement(15380..16627)
                     /locus_tag="VBC52_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO6881984.1"
                     /translation="MEKNTWKKLLVSTAALSVVAGGAIAATHSNSVDAASKTTIKLWV
                     PTDSKASYKAIVKKFEKENKGVTVKMIESNDSKAQENVKKDPSKAADVFSLPHDQLGQ
                     LVESGVIQEIPEQYSKEIAKNDTKQSLTGAQYKGKTYAFPFGIESQVLYYNKTKLTAD
                     DVKSYETITSKGKFGQQLKAANSYVTGPLFLSVGDTLFGKSGEDAKGTNWGNEAGVSV
                     LKWIADQKKNDGFVNLTAENTMSKFGDGSVHAFESGPWDYDAAKKAVGEDKIGVAVYP
                     TMKIGDKEVQQKAFLGVKLYAVNQAPAGSNTKRISASYKLAAYLTNAESQKIQFEKRH
                     IVPANSSIQSSDSVQKDELAKAVIEMGSSDKYTTVMPKLSQMSTFWTESAAILSDTYS
                     GKIKSSDYLKRLKQFDKDIAKTK"
     gene            16883..17911
                     /locus_tag="VBC52_000378"
     CDS             16883..17911
                     /locus_tag="VBC52_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6881985.1"
                     /translation="MVTIKDVAAKAGVNPSTVSRVLKDNASISSKTKERVKKAMEELG
                     YVPNVAAQMLASGLTQNIGVIFPPLVLPDRLSEPFFMEILSTLTNEAKEHGFTVSIAT
                     SMSSEELLEQVKLMHLQKRVDGFIVLYSDQKDPIREYLLKYKLPFVVVGAANGYENEI
                     TYIDNDNQLMGKSAIDYLYKKGHKSIQFVTDDLNSEVSEERYLGYFKGARKLGLNQKP
                     ALLFDRGNPQVLEEFINRVKEEETTALIVIGDTVSVRVIQFLSFYKLKVPDDISIMTF
                     NNSLFSHLIHPYLSTFDINVNNLGRTSVRRLIDIIKSPDKVFSETIIVPFTLEERESV
                     RDISKNNN"
     gene            18032..19528
                     /gene="malQ"
                     /locus_tag="VBC52_000379"
     CDS             18032..19528
                     /gene="malQ"
                     /locus_tag="VBC52_000379"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEO6881986.1"
                     /translation="MKKRASGVLMHITSLPGDLGIGTFGREAYAFVDFLVETDQKFWQ
                     ILPLTTTSFGDSPYQSFSAVAGNTHLIDFDLLTLEGFISKDDYQNISFGQDPEVVDYA
                     GLFEKRRPVLEKAVKNFLKEERATRMLSDFLQEEKWVTDFAEFMAIKEHFGNKALQEW
                     DDKAIIRREEEALAGYRQKLSEVIKYHEVTQYFFYKQWFELKEYANDKGIQIIGDMPI
                     YVSADSVEVWTMPELFKLDRDKQPLAIAGVPADDFSDDGQLWGNPIYNWDYHKESDFD
                     WWIYRIQSGVKMYDYLRIDHFKGFSDYWEIRGDYQTANDGSWQPAPGPELFATIKEKL
                     GDLPIIAENLGYIDERAERLLAGTGFPGMKIMEFGFYDTTGNSIDIPHNYTENTIAYA
                     GTHDNEVINGWFENLTVEQKAYAENYMRRLPNEPITETVLRTLYATVSQTTITCMQDL
                     LDKPADSRMNMPNTVGGNWQWRMRKEDLTENRKAFLKEITTIYNRGNK"
     gene            19540..21804
                     /gene="glgP"
                     /locus_tag="VBC52_000380"
     CDS             19540..21804
                     /gene="glgP"
                     /locus_tag="VBC52_000380"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEO6881987.1"
                     /translation="MTRNFTTYVGQQGKVLSELTNEEIYVELLNFVKEEAAAKSKNSS
                     QRKVYYISAEFLIGKLLSNNLINLGIYKDVKKELELVGKSIAEIEDVEPEPSLGNGGL
                     GRLASCFIDSISSLGINGEGVGLNYHCGLFKQVFRNNQQEAEANYWIENNSWLVPTDI
                     SYDVPFRDFTLKSRLDRIDVLGYKKDTKNYLNLFDIDGLDYNLIEKGITFDKTEIKKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLLIDEAIERGSNLHDLAEYAYVQINDT
                     HPSMVIPELIRLLTEKHGFEFDEAVSVVRNMVGYTNHTILAEALEKWPLEYLNEVVPH
                     LVTIIKKLDQMIREEQTNPEVQIIDEAGRVHMAHMDIHFSTSVNGVAALHTEILKNSE
                     LKVFYDIYPDKFNNKTNGITFRRWLEFANQDLADYLKELIGDSYLTDATQLEKLLTYA
                     DSNEVHDKLAAIKFKNKLALKRYLKENKGIELDEYSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKRGHFPSRKLTVIFGGKAAPAYTIAQDIIHLILCLSELINNDPEVNKYLNV
                     HLVENYNVTVAEKLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGKENIYTFGKDSDTIINLYETSGYRSKDYYDKDKVIREAVDFIISDDIVSLGNAE
                     RLKRLHDELVGKDWFMTLIDLKEYIAVKEQVLADYEDYESWNKKVIHNIAKAGFFSSD
                     RTIEQYNQDIWHSN"
     gene            22219..23592
                     /locus_tag="VBC52_000381"
     CDS             22219..23592
                     /locus_tag="VBC52_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6881988.1"
                     /translation="MLKKLFAPPQKRERLSAEEIKHKYPRYRVQVLISIFVGYMGYYF
                     VRNTTSILSGILNMSATEIGIITCASYIAYGLSKFISGLISDESNSKIFLPVGLFLTG
                     LVNVLIGVIPSVITSIWLFTIMYLINGWLQGMGYPPGARTLVYWYDNKERIKYATIWN
                     LSHNFGGAIAPILTGVGLALAGNDSLNQARAAYWFPGVVACLLAILVYFLQEDTPESI
                     GLPPIEEYHKEQYTNVVDSSNILEEPEVLGMGEIIKKYILPNTKLMWASLYSIFVYIL
                     RYGIVSWTPKFLATSVQDGGKGITATAGMGGFSLFEIGGIIGMLTAGYLSAKVFKNSK
                     PLTNVAFLVVAILLLAAYWFIPAGPQYMALDFIILLGLGASIYGPVMMVGLYAMELVP
                     KAAAGAASGLTGTFSYVGGATIATLAIGIIIDHFGWGVAFIIFGISGFAAIVCTLLSR
                     DKSLEYW"
     gene            complement(23747..24052)
                     /locus_tag="VBC52_000382"
     CDS             complement(23747..24052)
                     /locus_tag="VBC52_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6881989.1"
                     /translation="MTYELCLEYGTYPLRPVDAWADEINTAPAFITEDKKLLELLEEV
                     NTLFHELFLTIECSFHYIGHDFPEKRAKITQIYHVIIEHLSIHYPEYDIKIESLLMG"
     gene            24215..25201
                     /locus_tag="VBC52_000383"
     CDS             24215..25201
                     /locus_tag="VBC52_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEO6881990.1"
                     /translation="MSEKIRVLLYYKYVSIENAEEYAAKYLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWVHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENEAKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGESLPQVANV"
     gene            complement(25238..26365)
                     /locus_tag="VBC52_000384"
     CDS             complement(25238..26365)
                     /locus_tag="VBC52_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEO6881991.1"
                     /translation="MKHYQTIIIGAGAAGIGFGSAMQRLGLTNFLIIEKGHIGESFLR
                     WPRTTQFITPSFTTNGFGFPDLNAVIPDTSPAFSFEKEHLSGVEYARYLQLVAAHYNL
                     PIQNETSVLSIDKRDSLFVIKTSKGDFSADYLIMATGEFQNPNTIDIKGADLGMHYGQ
                     VDNFHIKSDNPFIIIGGNESACDALTHLVYLGNQVELYTDTFGRKESNPDPSISLSPI
                     TKERLKHIQDHKKEYYSISEGKKAIEIKQIGKQYQVTFDDGSTAESFHKPILSTGFLN
                     TCHLIDGIALFEYDKNQLPIVTEDDESTIVNNCFLIGPSLRQRDTIFCYIYKFRQRFV
                     PIINHIAQREGIILDEDTLEFFKENQMYLDNLACCDVNCDC"
     gene            26469..27488
                     /locus_tag="VBC52_000385"
     CDS             26469..27488
                     /locus_tag="VBC52_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEO6881992.1"
                     /translation="MFLLLCILSMWLMIFGVAFYYFGSLHQSLTSRIIYQFVLTVLIT
                     TIAWFMGAYFLGFEGHFKTVFQFQEADGKQIVNCLFQLCFALYAVVMLIGSIIDRVQT
                     KRLLLAVVSWLFLVYTPLAYLIWNSEGVFAKMGVLDFSGGMVVHLSAGLSSYILAHVI
                     GKSEHQHNKIKNDNLFLGMILITFGWFGFNMGPVGEWNSQAIMILLNTIFAIIGGGLA
                     WTLAAKWNGEEEKTGSLLNGIIVGLVTSTAGVGYLLTWQLLAVTFFASLFTYFVTDYV
                     AKAFAIDDVVSSFGMNGIGGLLGSLGVGLFKLSHMPVQLLALATTILLSIIMTYIISK
                     AIFRK"
     gene            27813..28619
                     /locus_tag="VBC52_000386"
     CDS             27813..28619
                     /locus_tag="VBC52_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6881993.1"
                     /translation="MSKKIILGILSLLSVVTLVACGSSDKQLQDKVEKKGKLVLAVSP
                     DYAPFEFKALVNGKDKVVGADIELAQAIADELHVKLEVSPMSFDNVLSSLQTGKADMA
                     ISGLSYTKERAKVYDFSKPYYTTENAVLVRKSDLGKYTSIESLKGKKIAVQKGSIEEG
                     VSKKSFKNSHVVSLTAMGEAISELKSGKVEAVDLEKPVAEGYLAQNPDLVLAKFALKT
                     GEGDAKAVALPKDSGKMLKTVNKVIKRLEKEDKYKVYISDAAKLTGNQVE"
     gene            28702..29205
                     /locus_tag="VBC52_000387"
     CDS             28702..29205
                     /locus_tag="VBC52_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEO6881994.1"
                     /translation="MVQGSINIEKYKDLALQKQSEHRKFLASLKKKAPKNLDRIVQQI
                     HQEVFQEIDCTACANCCKTLGPLFTEADISRISKHFRMKLSAFEDLFLGVDEDGDKVF
                     KAMPCPFLGEDNLCSIYDVRPKACREFPHTDRKKIYQINNLTIKNTLTCPAAYLFVEK
                     LKSRIGK"
     gene            29309..29485
                     /locus_tag="VBC52_000388"
     CDS             29309..29485
                     /locus_tag="VBC52_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEO6881995.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
     gene            complement(29582..29959)
                     /gene="mscL"
                     /locus_tag="VBC52_000389"
     CDS             complement(29582..29959)
                     /gene="mscL"
                     /locus_tag="VBC52_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEO6881996.1"
                     /translation="MIKELKEFLFKGNVLDLAVAVILGAAFNAIITSLVKDVITPLIL
                     NPVLKAAGVSNIAQLSWNGVAYGNFLSAVINFLIVGTTLFFIVKAANKVMAKKPAEEE
                     IIEVVEPTQEQLLAEIRDLLANK"
     gene            30083..31957
                     /gene="dnaG"
                     /locus_tag="VBC52_000390"
     CDS             30083..31957
                     /gene="dnaG"
                     /locus_tag="VBC52_000390"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO6881997.1"
                     /translation="MKKLLKKFTQKRKNDIIKCKTIMGYFCGGHDLAIDKEKISEIKN
                     SVNIVDVIGEVVGLTKTGRNHLGLCPFHKEKTPSFNVIEDRQFFHCFGCGRSGDVFKF
                     VEDYQHISFLDSVQVLAERSGIPLDTNFKGQVPKKPKANQSLLDIHRVASGFYHAYLM
                     TTNDGERARQYLAERGVTEDLIKHFQIGLSPGGQDFLYRRLAKEFDEKTLMSSGLFNY
                     SENSNQFYDSFNNRIMFPLTNDIGEVIAFSGRVWTQEDIDRKQAKYKNSRATPIFNKS
                     YELYHLDKARAVINKAHEVYLMEGFMDVIAAYRAGIENVVASMGTALTNEHVRHLKRF
                     TKKVVLTYDGDRAGQNAIDKSLELLSDMTVDIVRIPNKMDPDEFLQANSAEDFKQLLE
                     KGRISNTEFYIHYLKPENTDNLQSEIAYVEKIAKLIAKSPSITAQNSYITKVAELLPD
                     FDYFQVEQSVNNERLHHRSQQQASPSVQTSATVQLPQTGKLSAITKTEMQLFHRLLNH
                     PYLLNEFRNRDNFYFDTTEIQVLYELLKESGEITSYDLSQESDKVNRTYYMILEEQLP
                     VEVSIGEIEAVEKARDRLLKERDLRKQSQLIRQSSNQGDQEGALAALENLIAQKRNME
                     "
     gene            31965..33074
                     /gene="rpoD"
                     /locus_tag="VBC52_000391"
     CDS             31965..33074
                     /gene="rpoD"
                     /locus_tag="VBC52_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019787874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEO6881998.1"
                     /translation="MAEKKGNTTFNVQVADFIRNHKKQGTAIDDEVTEKLVIPFVLDA
                     DQIDDLLERLTDGGISITDKEGNPSTKYVVEGPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELAVAVAEGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLKDFMED"
     gene            33183..33524
                     /locus_tag="VBC52_000392"
     CDS             33183..33524
                     /locus_tag="VBC52_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEO6881999.1"
                     /translation="MSEVKRYSEEEVGKIKDRILEALEMVIDPELGIDIVNLGLIYEI
                     RFEDNGRTEIDMTLTTMGCPLADLLTDQIHDVMKTVPEVTETEVKLVWYPAWSVDKMS
                     RYARIALGIRS"
     gene            33614..34468
                     /gene="rfbD"
                     /locus_tag="VBC52_000393"
     CDS             33614..34468
                     /gene="rfbD"
                     /locus_tag="VBC52_000393"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEO6882000.1"
                     /translation="MILITGANGQLGSELRHLLDERTQEYVAVDVAEMDITNAEMVDK
                     VFEEVKPSLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKAAAKHDATLVYISTD
                     YVFDGEKPVGQEWEVDDLPDPKTEYGRTKRMGEELVEKYASKFYTIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQHGRPTWTRTLAEFMTYLAENQKDFGYYHLSNDAKEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLEKAKATGFVIPTWQDALKE
                     FYKQEVKK"
     gene            34585..35739
                     /locus_tag="VBC52_000394"
     CDS             34585..35739
                     /locus_tag="VBC52_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEO6882001.1"
                     /translation="MQDVFIIGSRGLPARYGGFETFVSELINHQKSSDIKYHVACLSD
                     KEHHTHFNFADADCFTINPPQLGPARVIAYDIMAINYALDLVKTHDLKEPIFYILGNT
                     IGAFIWHFANKIHKVGGLLYVNPDGLEWKRSKWSRPIQRYLKYAEKCMTKNADLIISD
                     NIGIENYIQTTYSNVKTRFIAYGTEINSRKLSSDDPRVKQLFKKWNIKSKGYYLIVGR
                     FVPENNYETAIREFMASDTKRDLVIICNHQNNPYFEKLSLKTNLQQDKRVKFVGTLYE
                     KDLLDYVRQKAFAYIHGHEVGGTNPGLLEALANTDLNLVLDVDFNKSVAGLSSFYWTK
                     KEGNLAKLINDSDQQQDLSTYGDRAKAIIQENYTWKKIVEEYEDLFLNES"
     gene            35729..36670
                     /locus_tag="VBC52_000395"
     CDS             35729..36670
                     /locus_tag="VBC52_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6882002.1"
                     /translation="MKVNILMATYNGEKFLAQQIESIQKQTFKEWNLLIRDDGSSDKT
                     CDIIRNFTAKDSRIRFINENEHHNLGVIKSFFTLVNYEVADFYFFSDQDDVWLPEKLS
                     VSLEAAKHKASDVPLLVYTDLKVVNQELNILQDSMIRAQSHHANTTLLPELTENTVTG
                     GTMMINHALAEKWFTPNDILMHDWFLALLAASLGEIIYLDLPTQLYRQHDNNVLGART
                     MDKRFKILREGPKSIFTRYWKLIHDSQKQASLIVDKYGDIMTVTDLELIKCFIKIDKQ
                     PFMTRLRWLWKYGYSKNQFKHQVVFKWLIATNYYNKR"
     gene            36672..37403
                     /locus_tag="VBC52_000396"
     CDS             36672..37403
                     /locus_tag="VBC52_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6882003.1"
                     /translation="MMKKILLVIPAYNEEGSIAKTVQTIVDFKASRSLPFELDYIVIN
                     DGSTDGTPELLDRLGLNHIDLVQNLGIGGCVQTGYLYANRNHYDVAVQFDGDGQHDIR
                     SIEDVVMPILNDEADFVIGSRFVDKKHQNFQSTAMRRLGINLISAAIKLTTGHKVYDT
                     TSGYRAANAALIAYLSCHYPVQYPEPESTARILKKGYRLKEVTANMFEREAGTSSISS
                     LKSIFYMTDVLTSIIIAGFIKEDDK"
     gene            37400..37753
                     /locus_tag="VBC52_000397"
     CDS             37400..37753
                     /locus_tag="VBC52_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEO6882004.1"
                     /translation="MSLALQIEMFILAIVLLYMIIRMVNKNTFSVKRSLPWLLVGLIL
                     VFISVFPGVVSFIAHHLGFALTMNFLLFGAILFLFCLELFDTSSNTKRDEQIKRLIQE
                     VSLLKKEIEDSTLDE"
     gene            37746..39479
                     /locus_tag="VBC52_000398"
     CDS             37746..39479
                     /locus_tag="VBC52_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEO6882005.1"
                     /translation="MSKKHIFICLFFMLFLVGCQNNKKVKTDKKVDITAYYYHNNTPQ
                     YSVQINAIHLQTALDKVRTGEFESKDFTHYTFDSIKRKYQVSGKKVLMDNNYRFGSGI
                     KNTRQDAIAIVRLLLEKNKDYLIYMNGLEEPSVLYNPENKRYKFTNNKGKAIGNIPVG
                     LTAFENSAETQEYVLKNIQKNETIYLGNTRVDNPRVTVNNKKRDTIGVEYGKRVTYRI
                     PIYSKQLTVRVSPNFVVDSTNYNYRLSQAPIIAEEGKEGRLYPIDSSISVDGDKIVLN
                     SSGDETEQKELLQNSLYKLHSIDKLDFDLTNANIKELEITGHVASKRDYNLSVAQEKK
                     GVIETKRSLISVDNQKQDQGIFVEDSKGYIKTPSLSSYGINFAMYDDNQNRLLQGSEY
                     ILGRLSADKKVFLYQPNGSWINTQKTIKSLNKEYLNQNAYHIRGNNIIGIDGYSNDID
                     LNPRLWAYNYKKQVKSNQSLFKLRGLSNKYKYFLIQTKSPKGYLQSNKPFYFTVNKDS
                     VSKAQFGNYNINGYIMDMQYNKQEYNALANTPKGQKTKKTFKPMTLVILFAVALFIFY
                     ITAIRFVFKRM"
     gene            39504..40328
                     /locus_tag="VBC52_000399"
     CDS             39504..40328
                     /locus_tag="VBC52_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEO6882006.1"
                     /translation="MKEMTVSEIREVQLEMLAYIDKVARDNKIEYSLGGGSLLGAMRH
                     KGFIPWDDDIDLMLERSQYERLMKALADANNSDFKLLHHSVEKNLWPFAKLYHTKSMY
                     LSKTDRIHPWTGIFIDIFPLDRLPESAEERQRFFKKVHSAAANLMCTTYPNFASGSRK
                     LYANARLILGLPRFIAYHGQAKKRAEIVDQVMETYNNQEVPYMGYTDSRYRLKEYFPR
                     EIFSEYEDVMFENIKTRKIKNEHAYLNQLYGGSYMELPPESKRESHSYYTWYWKEG"
     gene            40328..41050
                     /locus_tag="VBC52_000400"
     CDS             40328..41050
                     /locus_tag="VBC52_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEO6882007.1"
                     /translation="MMNIGVIFAGGVGRRMNTKGKPKQFLEVHGKPIIVHTIDIFQNT
                     EAIDAVVVVCVSDWLDYMNNLVERFNLTKVKAVVAGGETGQMSIFKGLEAAEQLATDD
                     AVVLIHDGVRPLINEEVINANIQSVKETGSAVTSVRAKETVVLVNDSSKISEVVDRTR
                     SFIAKAPQSFYLSDILSIERDAISKGITDAIDSSTLMGMYNRELTIVEGPYENIKITT
                     PDDFYMFKALYDARENEQIYGM"
     gene            41050..42108
                     /locus_tag="VBC52_000401"
     CDS             41050..42108
                     /locus_tag="VBC52_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEO6882008.1"
                     /translation="MMIKFSNKIVQEDLALISDQNYINWERLANKAVLISGSNSMLAS
                     YMVFLLAYLNETRNYQTQIIATARNIEKARDKFSDLVGKDYFTLIPYDVEERLEYDGK
                     VDYIIHAASNASPTAILSNPVSIIKANTIGTLNLLDFAKEKTIENFLFLSTREVYGTS
                     IKEVIDEEAYGGFDILATRACYPESKRMAETLLQSYYDQYKVPFTIARIAHSFGPGME
                     LGNDGRIMNDLLSNVIDGKDIVLKSSGTAERAFCYLADAVSGLFTILLNGEVGQAYNV
                     ANEDQPIMIKDLAQKLVDLFSDKNISVVFDIPKTMSAGYSKMGRTRLTMAKLEALGWK
                     REVSLESGILKTVQAFEE"
     gene            42110..43045
                     /locus_tag="VBC52_000402"
     CDS             42110..43045
                     /locus_tag="VBC52_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6882009.1"
                     /translation="MVKTAVLMATYNGEKFISEQLDSIRQQTLKPDYVLLRDDCSTDE
                     TVNVVNNYIAKYGLEGWKIVKNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDIWYL
                     DKNERQFAIMSDNPQIEVLSADVDIKTMSTEASVPHFLTFSSSDRISQYPKVYDYQTF
                     RPGWTIAMKRDFAQAIADNWTFGANISHDNLIETTASIIGNGYNLNESVGVHIRYGGN
                     ASGNSKFVLNWKSPKTQHITGLKTFVDFYETMQKVVNKYQNDVALNQRLAFEKERLEA
                     SKLSLIHNSAFVIKNWRLYNSNSGRMRDLLFAFKK"
     gene            43054..44001
                     /locus_tag="VBC52_000403"
     CDS             43054..44001
                     /locus_tag="VBC52_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6882010.1"
                     /translation="MVKTAVLMATYNGQGFIHDQLDSIRNQTLRPDYVLMRDDGSTDD
                     TVKVVEDYIKEHRLDGWSITSNDKNLGWRLNFRQLLLDAKRLSVDYLFFSDQDDTWYH
                     HKNKMQVDIMEERQDINLLSADIDIKKLSEEATIPNNFKFNGEETISKYPLDFSYHNY
                     RQGWTFCLRKSFVDQVMTFYSDNLILSHDNLFAGISGVIGSGYNLNRSVGEHKRHAEN
                     ASGNILNMSSSYERHLQELYFVLSYYIILVPFLEKSSSSQLREAKAYLDFNQQRYKNA
                     QNKDFVAILKQMMTKKRFYDSFSNRIRDLVFLLKDIKRS"
     gene            44003..45367
                     /locus_tag="VBC52_000404"
     CDS             44003..45367
                     /locus_tag="VBC52_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEO6882011.1"
                     /translation="MNLKKVRLFEKFFLDKNLHKTYFILGLTITIVLSLLMPFFNEPD
                     GQYHLAVSGRISNNIIDTSYYGEYNIDSGMRRQSTSYRDGTRFEKYYLNKANFVDGNH
                     LPREINYSKHNFVFWGHLVPAVGLFLGKFVYPSMGVMITVARLFSSFVSITLVALIIR
                     YVKKGKLIYFAIFLSPVVLNSFASLSYDSTGYIVFGAFLSLLINLLVDKSLTKNLSIA
                     LIGLILLVIVACKQNYWLFLLLVIPVLLQVNNPKLMGYNQKLSHLFQGLKSKKLFWLC
                     LTFLSLAIVYVLTARHGGVLAVLRRYVMTFGYNYAGINILSNDITSWLASPYPSQNFI
                     PTWVSSVWYLMVFLVWFSEEKFVKSKNMGFLFLASFFVGILGVFFIMLEYRGAPTSYI
                     EGVQGRYFTVTLLLVQVFASSIPARLNEAGRKVVPYALTGLVIVSNALLLFDTVISLI
                     MRRN"
     gene            45367..46791
                     /locus_tag="VBC52_000405"
     CDS             45367..46791
                     /locus_tag="VBC52_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEO6882012.1"
                     /translation="MKLLKNMFYNTSYQLLTLLLPLVTVPYVSRVLSPQGIGINAYTS
                     SIVMYFTLFGALGISLYGNREIAFVQSNKYKRSKIFWELVVLKLASVSIATLLFFGFV
                     LLTNEWQLFYLIQGINLLATATDISWYFIGVEDFKIIVIRNTIVKLITVVLTFLVVKT
                     PDDLALYMFLIAFASLLGNLTVWHHLKHEIIKIPFSRLDVLVHLRPTLMLFLPQITMQ
                     IYLSLNKSMLGAMDSVVSAGYFDQSDKIIRILFTIVSAIGGVFLPRLSSLFSSGKEKQ
                     AKALLLKLVDLSNAISMLMIAGVVGVSSTFAVFFFGKGYEVVGPLMAVESLMIICISY
                     GNALGTQYLLASRRTKAYTMSAVIGLVANVVLNILLIPILGAMGAIISTVITEFIVSL
                     YQAISLRDVFTFKELTRGMLRYLIAATLSGAVLYYINTQMSVSLVNYVIQSLVAVTIY
                     VGIVFITKAPVIQLFRDFRKEHRT"
     gene            46788..47636
                     /locus_tag="VBC52_000406"
     CDS             46788..47636
                     /locus_tag="VBC52_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6882013.1"
                     /translation="MKYLAGIVTFNPNIERLDQNIRAIYPQVSHIYIVDNGSKNKEEI
                     SQLVADYNEEGHLTVDYLTENKGIAYALNCIGQFAVAQEFDWFLTLDQDSVVLGDLID
                     NYENYLHLPKVGMLSCLYQDMNRENLVMQEFDYKEIEECITSAALMKTSVFEETSGFA
                     EEMFIDFVDSEMNYRLSEMGYKTYQVNFIGLLHEIGHSSRVKKFGHVFHVLNHSPFRK
                     YYMIRNAIYIIKKYGKKKRYKYLVFMRNEFVRVLVAEEQKSKKIVAMIKGLKDGLLMK
                     VDKYGK"
     gene            47626..48765
                     /locus_tag="VBC52_000407"
     CDS             47626..48765
                     /locus_tag="VBC52_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO6882014.1"
                     /translation="MENKVKTVAVFSGYYLPFLGGIERYTDKMTADLVKRGYRVVIVT
                     TNHGDLPIIDEDKGRKIYRLPTKNIVKQRYPIINKNREYNTLMKYVSDENIDFVICNT
                     RFQLTTLEGLSFAKNHHLPSIVLDHGSSHFSVNNRFLDFFGAIYEHLLTARVKHYRPD
                     FYAVSKRSVEWLKHFNIEAKGVIYNSVSESLGSDFAGTAYLEKSADDIFITYAGRIIK
                     EKGIELLLEAFSMSQYSENVYLQIAGDGPELAHLKEKYQSKQINFLGKLNFEQTMSLM
                     AQTDIFVYPSMYPEGLPTSILEAGLLSSAIIATDRGGTVEVIDSPELGIIMEENTQSL
                     HESLDLLVKDKALREKLQQNIAKRIKEHFTWEKTVEKLDYIIQKN"
BASE COUNT        16254 a         7225 c         9402 g        15902 t
ORIGIN      
        1 cttgaagaga acaaattgat agagaaagag aaattccatt gaaattacta tatatttttg
       61 aaaaaaatat tattcttcgt aaaatattga taacattttc attaataatc atttttttac
      121 ttggtcgcta tgttccaata cctggtgtct tgatttctgc ttataaggga caagacaata
      181 actttgcgac gctttattca acagtaactg gtggtaattt gtctcaggtt ggggtctttt
      241 cattgggtat tggtcctatg atgaccacaa tgattttact aagactattt acgattggta
      301 aatactctag tggagtatct caaaaagttc aacagtttcg ccaaaatgtg gtgatgcttg
      361 tcatagcaat tattcaaggg ctagccatag ccatatcttt tcaatatcat aatggttttt
      421 ctctgaccaa attattactc gcgacaatga tacttgtgac tggtgcatat attatttcct
      481 ggattggtaa tctaaatgcg gaatacgggt ttggtggaat gactatttta gtggtggtag
      541 gtatgcttgt tgggcaattt aataatattc ctcttatttt tgaattattc caagatggtt
      601 atcaacttgc tatcatctta tttcttttgt ggacactagt agcaatgtac ttaatgataa
      661 cttttgaacg ttcagaatat cgtataccag taatgcgtac ttcaatccat aatcgtctcg
      721 ttgatgatgc ctacatgcca ataaaagtaa atgcttcggg ggggatggca tttatgtatg
      781 tttatacctt actaatgttc ccccagtata ttattatttt actaagaagt attttcccaa
      841 ctaatccaga tatcacatca tacaatgact attttagttt atcttctatt cagggagtgg
      901 ttatttatat gattttaatg cttgttttat cagttgcatt tacttttgtg aatatcgatc
      961 cgactaaaat ttctgaggca atgcgagaaa gtggtgattt tattcctaat tatagacctg
     1021 gtaaagaaac acaaagttat ttatcgaaaa tctgttattt atttggaact tttagtggtt
     1081 ttttcatggc tttcttagga ggggtaccat tattatttgc tcttggaaat gataatctta
     1141 ggacagtttc atctatgaca gggattttta tgatgatcac agggatgtca tttatgatat
     1201 tggatgaatt ccaagttatt cgtattcgta aacaatacac ttctgtattt gaaaatgagg
     1261 agaattaatg tttcatttta ttccatcttg gtataatgaa aatcgtacct ggtatgataa
     1321 taattacttg tggtatttca agccgactaa tgtcggtttt gatgatacta tcaaccaaat
     1381 gaagatgttt gattatgctg gaaaagagag tagattagtt gtcttaaact atatgcccaa
     1441 tttacgttat tatttacatc gctatgactt gttagaatca ggttattatt cagtttttga
     1501 tgatattcaa gaaatcggga atgtaagaca gcaaatgatt gacttccgac aactcaattg
     1561 gcctgaaggg gtggacttta cctatactcc gtttatagtt ttggtaaaaa agtcaggaga
     1621 tttaattgct aaggttcaat ttggtgaaga aggaaattta acgcatattg attattttgc
     1681 aaatgaacaa atagctaaga aatacctctt tgatgataga ggatttctat ctagtattct
     1741 ctattatgat aatggtggtg aagcctatca agattattta gcatcatcag gggaaaggat
     1801 tatgagagaa tacctaagag aaggtgacca ccatgttgaa attaatccta agaaagctat
     1861 tcatttttta aagttatctt actccgacat tgaagagttg attagagaga aatatctgac
     1921 ctatctccat aaagaagtta gtaaatcaga tactatcatt gctagcttta atcaagttca
     1981 taacgcattt attgttggga acactagtaa aggaaattta atattatctg ttttttcaga
     2041 gaggaataat gctcacaatg tattggagga ttactcttcc ttatctcgag cagacgctat
     2101 tatttgtgat agactagata ttgctgcaca attgaaagaa aaaatagata aaccagttgt
     2161 tcatgtttct ccctttgata cgcgtttagc tttgggaaaa agtaatcaag ttagagattt
     2221 agaaatatat tttgtcatag atagactttc ccataaagaa ttgcaaaagt ctttaacgtc
     2281 cttatataag gttatgctca aaaataatga tataaaagta acatttgtca gctacgaacg
     2341 agaatttgaa agtcggcaat tgacgtatga ctaccttaaa gaagcaacaa aagtttttga
     2401 tcagaaattc tttagtttat cagacgatga tgccaatcaa gttcttatgc aatctgatga
     2461 agtagaaaag gaaaaaacac gcctttcttt tacccatcct ttgtcagaaa cagatatcat
     2521 aaaccggtta gagtatgtcc gtttgattat tgatatctcg aaaataccag acttatacac
     2581 acaaattgca gggataagtt caggtatccc tcagataaat acaatattaa cagaatttgt
     2641 tgaacacagg aaaaacggtt acgttattga agagatccaa gaattggaaa aggcaattcc
     2701 ttattattgt gaacaattaa ctaattggaa ccgttcttta atttattctg ttgataaaat
     2761 caatgactat acaggtggtc aattggtaga aagaattata aatagttatt aaataaatat
     2821 gtcaaaaata aaacttacaa ttcttcaagt tggtgaagag aactgggcaa ctaaagagaa
     2881 tatccctaat gatatggaat ggctctttat taaaccagat caaatttctg actttgtaac
     2941 tacagaaaat aattatctta catcgtcaaa gttattacaa aaactaccac gaaaaatatc
     3001 tgctcttttg ttgacagagc aaacttatgg accagagtta tctagtttat cgtcattctt
     3061 tgaggtttat gaagtatttt atcctaaaga taaacatgct acggggataa cagaagagtt
     3121 tttacgctcg aaaatggccc aacgatatga tagtagctct ccagctcagc taattcgtca
     3181 attttataaa gggcttttta taggacaata cggagagaaa cttcaagttt cacaaataca
     3241 aattagaaat gattttgaag gtgtagtaaa ctatcaggga aataactatc ttgaattaga
     3301 agggcaattt ggtgaggact atagtttttt acttaatttt gcttacaata ttccattttc
     3361 atcggatttt tataatgagt tatttttaga acacattata gagggtgata tagatattcg
     3421 attggttatc tcgttgattg ttgatggttc tgttgatgat attgctaaag agtggtactt
     3481 tgagaaggaa gacctaaatc aacttatttc tttagagtcc gatattagtg gtagccttgc
     3541 agttaaactt tttgctaagg gaaagggtat cattaagtta ggtccattgc accgtcgtaa
     3601 tggtagagga ggattgggca catttctact aggaggagaa cgtcacatag atgctatagg
     3661 tcatgaattt atgacttatt ttgatccagt agatttcaaa ccacctctaa ctgtttattt
     3721 ttcgggattt cgttcagctg aaggctttga gggattctgg atgatgaaga gtatgaagac
     3781 tccattcatg ttaatttgtg atccacgatt acaaggtggt gccttttata taggaagcaa
     3841 agaatatgaa caaaaaatag ttgatgctat tcaagaaaaa ctggcctttt taaattttag
     3901 ttctgatcag ttaattttat ccggactttc aatggggact tatggtgcta cttaccatgg
     3961 tgctaagcta aatcctcatg ctattattat tggtaaacca atatttaatt taggaacagt
     4021 tgctcaacgt gaaagattag agagaccaga tggatttgct acttcattag atattcaact
     4081 actcaatcaa ggtgacttga ccagttcctc ttctgagaag ttaaataacc ttttttggaa
     4141 atctatagaa gaaggagatt tttcaaatac gacttttgcc ttagcctata tgaaaaacga
     4201 tgactatgat gcgactgcct tttctgattt attgcaatat tttagaggaa agaagcataa
     4261 gatattaggc agaggttggg atggccgaca tggtgattgt tctgcagaag ttggtgcatg
     4321 gtttacaagt cagtataggc gaatgctatc taatgatttt ggaaggaaag aagaatgagt
     4381 acgataagct atatttattg ggatgatttt tcaaggtttt cttataattt tggaacaaaa
     4441 ttacaatttt tgggaaaatc agtatgcttt gaaaatccat tagctccttc tagcacaaac
     4501 ttgtatacat ggagctccca gactaattat cagtcaaaaa gaatttctcc taacttacct
     4561 ttattaagaa agggaactcg ttatagcttg tcattaaatg cagaactcga tttagttggc
     4621 tctctttttg ttcgcattga attttacaat cgctttaatg agtctattgg ttttgaactg
     4681 ttaaaaaaag atagtattat ttttatctat cctaaagagg cttataccta taccattagc
     4741 ttgattaatg caggttgtag cgattttact tttcattatt tgaaacttga ggaagttaca
     4801 gatagcgtta ttcaggagaa aaatctatcg acagagttta ctattgaaga acatcaagac
     4861 gtttttaatc ttcttttggt cgagaaaaaa gatagtgtgt atattaataa aatagaaagc
     4921 atttctcaat tgcaacagaa agtcgagctg gtcagcaatc cctcattaaa ttctgatagt
     4981 ttgatattac ctgagttgga aaaggggtta gaagatgcat taaaagtttt tcctaacatt
     5041 aagattaatg tcattgcata tggaacacaa ggtaattttg ctgctttata ttatgctaaa
     5101 aaatttccca gaataactgc ctatattaat gattgttttg ctcctttcgg aattttactt
     5161 aaatccttac cacacttgac tgccaaacaa caaatatttc ttagagaagt ctgggatacg
     5221 agagagacgt ctccaaatgt aaagcactat ggattggttt cagaaaattc ctctttaaat
     5281 ttagtatcaa tgatcttatc tggtaatgaa catctacctt acctcacact tttaaaaaag
     5341 caggacgaca attatgacag cctttaatag tttattttcg ttagataaga aacgtttaaa
     5401 aaaacttcag cgtactttaa atactatcaa tagtttaaag gaccaaatgg caacactatc
     5461 aaatgaggag ttgcaagcta agacaactga atttagaaaa cgtttagtta atggggaaac
     5521 actagatgat atttgcgctg aagcttttgc tgttgttagg gaagctgata agcgcgtatt
     5581 aggtctattt ccgtatgatg tacaggtaat cggtggtctt gttctgcacc aaggaaatac
     5641 tgctgagatg aaaactggtg aaggaaaaac attaactgcc actatgcctt tgtatctcaa
     5701 tgctttagaa gggaaggggg caatgttgct gactaataat tcctaccttg ctattcgaga
     5761 tgcggaagaa atgggaaaag tatatcgttt tttaggattg agtgtcggtg tgggagtttc
     5821 tgataatgag gaagaggata gagatgctgc gactaagcga gctgtttata gttcggatat
     5881 tgtctatagt acaagtagtg cactaggctt tgattattta attgataacc tagcttcatc
     5941 taaatctcaa aaatatatgc ccaagttaca ttatgctatt gtagatgaag ctgacgcagt
     6001 tcttttagat atggcccaga ctcctttggt tatttcaggt tcaccccgag tacaatctaa
     6061 tctctataaa atagcagatg aattaattct tagttttgaa gaacaagttg attattattt
     6121 tgacaaagag cgtcaggaag tgtggataaa aaaccagggt gttcgtgaag ctgagagata
     6181 ttttagaatc cctcattttt ataaacagtc taatcgagaa cttgtgcgac atttgaactt
     6241 atctttaaaa gcacataaat tatttgaacg aggaaaagat tatgtggttg acgatggtga
     6301 aatcaagttg ttagatgcga ctaatggccg tgttttagaa ggtactaaac ttcaaggagg
     6361 tgttcatcaa gctattgagc aaaaagagca tcttaatgtc acaccagagt ctcgagcaat
     6421 ggcttccatt acctatcaaa atctttttag aatgtttact aagttagctg gaatgacagg
     6481 aactggaaaa actgctgaaa aagagtttat tgaggtctat gatatggaag tggttagaat
     6541 tccaactaat tctcctgtta gacgaattga ctatcctgat aagatttata caaccttacc
     6601 agagaaaatc tatgcaacga ttgaatttgt taaacaggtt catgatacag gacaaccaat
     6661 cctactagtt gccggttctg tgagaatgtc agagctattc tcagaattat tattactaag
     6721 tggtatccct catagcctgt taaatgctca aagtgcggtt aaggaagcgc agatgattgc
     6781 agaagcaggg caaaaaggag cggtcactgt tgcaacgaat atggctggtc gtggtacaga
     6841 tattaagtta ggtaagggag tatcagaatt aggtggtctt gcggttattg gaacggagag
     6901 aatgaagagt caaagaatgg acttacaatt aaggggacgt tcgggtcgac aaggggatat
     6961 tggttttagt caattttttg tatcatttga agatgatcta atgatagaat caggtcctaa
     7021 atgggcgcaa gcttacttta ggaaaaatcg tgacaaagtt aatccagaaa aacctaaagc
     7081 cttaggtcag agaagattcc agaagttatt ccagcaaact caggaagcta gtgacggtaa
     7141 gggagagtct gcacgtagtc aaacgataga gtttgattcg agtgtacagc ttcagcgaga
     7201 gtatgtttat cgtgagagaa atgctttgat caatggagaa agtggacatt ttagtccaag
     7261 acagattatt gatacagtga tttcttcctt cattgcttat ttagatggtg aagtagaaaa
     7321 agaagaactc atttttgaag taactcgttt tatatttgat aatatgtctt acaatcttca
     7381 agggatttct aaagagatgt cacttgaaga aattaaaaac tatttattca aaatagccga
     7441 tgagattctt cgagaaaagc ataatttgct tggtgatagt tttggtgact ttgaacgtac
     7501 tgctgcttta aaagcgattg atgaagcttg gattgaagaa gtagattatc tacaacaatt
     7561 acgtactgtg gcaacagctc gtcaaacggc acagcgtaac ccggttttcg aatatcataa
     7621 agaagcatat aaatcctaca atatcatgaa aaaagaaatt cgtgaacaaa cttttagaaa
     7681 tctacttttg agtgaagtat ccttcaatga gaatggagat cttcaaattt attttatata
     7741 agagatgaaa gataatgaca gtttataata taaaccatgg aattggttgg gcaagtagcg
     7801 gtgttgagta cgctcaagca taccgaggtt ctgttttgag aaaacttgga attgatgcta
     7861 agtttatttt tacagatttt ttttcagctg ataatattat tagcctcacg cgaaatatag
     7921 gttttcagga taaagaaatt atttggttat ataatttctt ttcagatatt gagcttgcac
     7981 cgacaacttt tagtatagaa gatttgcaaa aacaatatct tggaaaactt gttcgaaaag
     8041 aagatagagg tcgggtaatc aagtttttct acgaagatga aaatatctat ttaacagttt
     8101 accttgataa ctttaacaag gataaagtgc atcgtgtaga aatcgtttca aataacaacc
     8161 ttatcagaaa agattattat agttatacac gtactttctc agagtattac tatcctaaag
     8221 atggtgtagc tcacctttat caacgtagat tctacaatga agatgaaagt acagcctttt
     8281 tggagatggt agaaaatagc tcaagtcgtt ttattatcaa tggcagactt ttaccatcca
     8341 aagtagcttt ttttgattat tttttggaat caatgacttt cacttcaaaa gatattattc
     8401 ttttggatag aggaactgat accgctcaga gtcttttgcg ccatggtaaa cccgctaagc
     8461 tagggactgt tgttcatgcg gaacatttta gtgagaatgc tgtgactgct gacactattt
     8521 tatggaataa ctattacgat tatcagttta ctaacgctaa tagatttgat ttttttatta
     8581 cctccactga taaacaaaca gaacttttgg aacaacaatt taaacaattt acaaatcata
     8641 atcctagaat tataactatc ccggtaggct caattgacaa tcttaaaatg ccaatggata
     8701 atcgccgtcc gtactctatt ttgacagctt cacgtctagc tagtgaaaaa catgtagatt
     8761 ggttagtacg tgcagttatt aggataagag aaattcttcc tgaagtgaca tttgatatct
     8821 atggatcagg tggagaagaa gaaaaaatta gaaatattat aaatgcagcc aatacaacgg
     8881 aatacattcg attgatggga catagaaatc tctcgaatgt ttatcaaaat tatgagttat
     8941 atttgacagc ttctaaaagt gaggggtttg gcttaacttt acttgaagct attggcgcag
     9001 gacttccttt gattgggttt gatgttcgtt atggtaatca aacttttatc aaagacggag
     9061 aaaatggtta tctaattcct cgatttgata tggatgatga ggaagctatt gtagaagctt
     9121 ttaaagagaa agtgttacaa ttattccaac aggatcaaaa ggctttacga gaagcttctt
     9181 acgccattgc cgaaggattc ttaacaagtg aagtagaagg aaaatggtat aacttagtta
     9241 aggagttggt acaagatgat taacttattt gatagttaca ctcaatcaag ttgggattta
     9301 catttttcct tgataaaatc aggttatatt aaccctacta tagctttgaa tgatgatggt
     9361 tttttaccag acgacgtaac atcaccctat ttatattaca ctggttttgc aaagataggt
     9421 gctggccgcc cactttacta caatgaactt agagttcctg atacatggga aattattggt
     9481 tttagtagtg gtgctgatat tgttgactta ggtgtcaaaa agggcagaat tatttatgca
     9541 aacccaaatc ataggcgtct aataaaagag gtagactggt ttgatgagca aggtagagtc
     9601 attttaaaag atcgctttaa taagtttggg ttctgttttg cacaaacctt ttataacgtg
     9661 gatggtcaag cgattcagac gtcatattat aataaggatg ggcaagaggt tatatcagag
     9721 aaccatatga caggggatta tattttaaat gataataatc agtttaaggt ttttaaatct
     9781 aaagttgaat ttgtcataaa ttatcttcaa gaagcaaaat ttaacttaga taggatcttc
     9841 tataattctc tgtcaacacc atttttagta agcttttacc ttaatcgtct agagagtaaa
     9901 gatgttctct tttggcagga gcctctcgta gatgatattc caggaaatat gcgcttatta
     9961 ttgaacaatc cttccccaaa cactaaaatt gtaattcaaa gttatgaagc ttatgctaat
    10021 gctatgaggt tattaactga cgaagaacaa aaacaagtta gctttttagg atttatgtat
    10081 cctcttaaag agacagaaaa attacataac caagctctta ttttgacgaa ttctgatcaa
    10141 atcgaagcat tagaaagttt agtgacttct cttcctaatt tgacttttaa cataggtgct
    10201 ttgaccgaga tgtctagtga actcatgaat tttggcaaat atgataatgt tgtactctac
    10261 ccaaatatca caacaaatca aatacaatat ttatcaaata tttgtgcttt ttatcttgat
    10321 attaatcatc ataatgagat attatcagca gttcgctctg cttttgaaca tcaacagtta
    10381 atttttgctt tcgaagagac tagtcatcaa attcgttttg tatcacctaa aaatattttt
    10441 ccaaaaaaag atatttttac atttatttcg cacttgcagc cactaattgg caataaatgt
    10501 aatatagaaa aagcattaaa acagcagctt gaagattgtc atgtttcaag ctctactcag
    10561 tatcaatctg taattaatta aggggaaaat tttgaaaaaa aagaatttac ttcaggagag
    10621 tattaataaa cgtctatctg aagagcgata tcaaattcct aaagaaaaaa gagagaaaaa
    10681 acgatttgac atgcagctta ttctgattat ttctatttta atagggttaa tcatgtctat
    10741 tatcggaatt attcgatttt tcttaactta cagtagttaa gttactatcc tttattttgt
    10801 agaaaaattg ataaataaat ggtatcgtaa ctctatcact aaatttatat actagaggaa
    10861 ataatgtcac gcagtcaaaa agttatttta actaatatgt gtcttattga agataataaa
    10921 ggacgtattg tgatgcaaat ccgtgatcct aaacgttatt cttggtcagg agctgcttta
    10981 ccaggcggcc atattgaaga aggagaaagc ttgtatgatg ctctcgttcg agaagttcgc
    11041 gaggagacag gtttaacaat aagtaatccc aaattagttg gtatgaagca ttggtacaca
    11101 gatgaaggca tccgttattt ggtattcctt tataaaacat atgatttttc aggtgaatta
    11161 caatcgtcag atgaaggtga tgttatatgg attgaacgtt cacaacttga taagattgaa
    11221 ctagcatatg atatgcttaa ccttttgaga gtatttgatg aagatgacat ctcagagatg
    11281 ttttatagta agcgacttga agatggtttt ataagaaaat tttattaaac aaaaaagaac
    11341 taggttacat tcctagttct ttttagatgt ctttatttaa ctccagacat tagtttttca
    11401 atgcgtggaa cataaaataa gaggataaat ccaaatacaa ctgttatacc accaacaaca
    11461 ccataataaa gaatttgagt atcaggtgtg tacaatttaa cgatttgagc gttaatagct
    11521 tgtgctgcag cattacttaa gaaccagatg ctcatcattt gagcttggaa tgctttaggt
    11581 gcgagcttac tagttgctga taatccaact ggtgagatta gcatttcccc aacgatgaca
    11641 atagcccaac tcattgttaa ccaaagaggg cttactttag cgttaactcc aaagagtaaa
    11701 cctggtagca tcatccataa gaatgaagct ccagcaaaga aaagtccata cgcaaatttt
    11761 ttaggtgatg aaggttgctt acttccccat ttagcccata accaagcaaa gaatggtaca
    11821 tagagcataa tgaagagagg gttcatgctt tggaaataac ttgatgggaa attaatatga
    11881 tgcccaaaga agttaaggta aagttttgtt tgttcatctg cgaataaggc taagacaact
    11941 gaaccttgtt cttcaattga ccaaaataga attgaggcga taaagagagg gatatatgcc
    12001 catactcgag aacgctcaac agaagaaatc tttttactac ttaaaatctt gataaaataa
    12061 ataattggaa tggcgattgc aataatacta aaaatatcga taattacttc aattgttagc
    12121 atatgcatca agtgaagaag aataagtaca ataacaatac caataaggat aagcccaact
    12181 tttttatata gagaggactt ttcttcagga cttaaaggat cgtttggtct gagactctct
    12241 tcagtcaaat atttttttcc atctaaggta tattggagga gaccaaagaa cataccaata
    12301 gcagcaagtg agaaaccaag atggaaattt acttcttgtc cgaggtaccc cactacaatg
    12361 ggtgaaataa aagcaccaag gttaatacca aaaacaaaga tactaaaacc agcatcacga
    12421 cgagaatcat tttctccgta taaattacca accatctctg atacatttgg ttttaatagt
    12481 ccagtaccaa ggataattag agcaatcgaa atgaatagag ctgtttgacc aaatggcgta
    12541 gctagagcaa tatgacctag catgattaga ataccaccat acagaacagt tttacgacta
    12601 cctagaatac ggtcgctgac aaaaccacca attactgatg agagataaac cagtgaacca
    12661 tagacagcca tgattgatgc agcgacagtc ttgtccatac caaggccacc ttgagaaaca
    12721 ctatagtaca tatagtacag caaaatagca cgcataccat agtatgagaa cctttcccac
    12781 atttcagtaa agaaaagagt ggacaatcca cgaggatgtc caaagaatga ttttgttttt
    12841 tccatgaaaa acgctccttt tgtagataac actattaata atatagtaaa tagaagcaac
    12901 tgtcaaatta cttagcacta tcaagttatt tttttgtaga ggttaacacc cttggtaaac
    12961 caagtttttt aactatctag cctagaaaaa ggcttgaatt caatagaact caagcctttt
    13021 ttagaaaaga aaccacagtg aaataaaaat atcgtgtctt taaattatct gaagattatt
    13081 ttacagaccc acctgtaata ccttcaacat agtatttttg catgaacatg aaaagtattg
    13141 tgattggaat tgcaataata acagatcctg ctgtgaatga catgaaccac tggttgatgg
    13201 tatcttgttg caacattgaa aagagaccaa tagcgactgt gtacttgctc gtagcatcac
    13261 ctaaaataac ttttgcaaag atgaagtcca tccaaggccc cataaaagcg attaatgcag
    13321 tataaacgat aatgggtttt gacaatggta gggtgatttt aaggaaaata tccaaacggg
    13381 tcgccccatc aatcatagca gactcatcca aagagtatgg aatagtatca aagaaacctt
    13441 ttgcgatgta gaaggttaat gctgctcctg cagaatagac aaatattaaa gcggttaatg
    13501 tttggtctag atttagagct tttaggatgt agtaaactgc aatcattgac atgaacccag
    13561 gaaacatatt caagactaaa gctagtttta ggaaaccatt acggtgtttg aatttgattc
    13621 tgctgagaga ataagccatt gctacagtga ttaaagttga tagtatacaa gtacatgtag
    13681 caacaaataa tgtatttaag aaccattgac caaatgggaa tgtgttttgg gtgaagagtt
    13741 tagcgtagtt gtctaatgtc caagttttag gaataaagta gttgacaaag gcacttcctt
    13801 caccacggaa gcttgtgaga acgacccaaa caatgggaaa gagccacata attgatagta
    13861 ctatcaataa aatataaaca aaagtaaggt taagacgttt tttcttattc attatttagc
    13921 tgccccttcc ttgtaagaat ttgttcttgt gtaagctagt aaactaaata ctgctgaaag
    13981 aataaagatt aagataccaa caacagatgc taagttataa tctgcagcag ttactgtcaa
    14041 attgtatagc caagtgacaa gtaaatcggt gctacctgct tggtagtatg ttgagtttgt
    14101 tggtccacct tgagtgagta ggtagataac gttgaagttg ttaatatttc cgataaattg
    14161 ttgaattaaa gtaggagtca taatcaacaa aatttgtggg aaagtaattg attgaaagac
    14221 ttggaactta ttcgccccat caatttcagc agcttcaatt tgttctgcag gtaaattcat
    14281 gatgatacca gtagcaacaa gcattgttac aggaatacca acccacatat taacaaagat
    14341 gattgagaat ttcgcccaga ccggatcaga aaggaatgga aggggatgtg ctgaagaaat
    14401 aagtccccat tttattaaca aggcgttaac cggtccagcg tcacttaaga ggtttctcat
    14461 gattaagaga gagataaatt gagggacagc catggtaata acaaaaatag tacgccacat
    14521 ctttttgaat ttcaaacctt ttgtattaat tagtaaggca agaataatac caaagaagaa
    14581 attcgttaca gtagcgaaaa cagcccaaat taatgtccaa gatagaacag ggaagaaagt
    14641 actggccata cgcccgctaa agatattacc gaagttagca aaaccaaccc agtcaaataa
    14701 actttttggt ggtaagtggt tatgatcata gttagtaaat gccaaacaaa tcatataaac
    14761 taacggaagg attgtaaata gaagcacccc tattaaagga atagacatta aagtcatatg
    14821 gaagcgtcca tctgtcaagc ttttaatatc ttttttgaag gatggaattt ctcttccttc
    14881 ttctttcaag gtcaataaat gtgctgcgct tttaatgttt gaccagtaaa tataggcaaa
    14941 tacgcaacag aagataagtg aagctaatcc aaaaattagc attaacatag agttatcacc
    15001 ttgtgtcgca acttggattt taataccatc cacagtttta gttgtcatac cttgttcttg
    15061 ggtccctaag gaaataagac cttttacagc tgggataact tggtaaacaa aggtaattaa
    15121 aaacagaatt tcactgatta aaaataaaag tcccttgaca atttgtttat ttttgaggtt
    15181 agcaagtccc ataattaaga atgatagttt agttgctaaa tcaccttttt gaaagacctc
    15241 ttttagggac ataccatcaa gtgagatcag ttttttattc ataatggtct cctttaattg
    15301 ttattttaca aaataagaga tgggaagcac tctcaattga aaggaagagt actcacctca
    15361 tctcattact ctagacgtac tattttgttt tagcgatgtc tttatcgaat tgttttagac
    15421 gtttaaggta atcgctagat ttgattttac cactgtaagt atcgctaaga atagcagcac
    15481 tttctgtcca gaatgttgac atttgactca acttaggcat aaccgttgta tatttatctg
    15541 agctacccat ttcgataact gcttttgcaa gttcatcttt ttggacgcta tcagaagatt
    15601 gaattgatga gttagcagga acgatatgac gtttttcgaa ttgaattttt tgactttcag
    15661 cattagttag atatgcagcg agtttgtagc tagctgagat tcgtttagtg tttgaaccag
    15721 caggtgcttg gttaacggca taaagtttaa cacccaagaa tgctttttgt tgaacttctt
    15781 tgtcaccgat tttcattgtt gggtaaacag caacaccgat tttatcttca ccgacagctt
    15841 ttttagcagc gtcgtaatcc catggtccac tttcaaaagc atgaacagaa ccatcgccaa
    15901 atttagacat tgtattttca gctgtcaagt tgacaaaacc atcatttttc ttttgatctg
    15961 caatccattt aaggacagaa acacctgctt cattacccca gttagtgcct ttagcatctt
    16021 caccagattt accaaataaa gtgtcgccta cagaaaggaa aagagggcct gttacatatg
    16081 agttagctgc tttaagctgt tgaccgaatt tccctttgct tgtaattgtt tcgtatgatt
    16141 taacgtcgtc agcagttaac tttgttttat tataataaag aacttgagat tcaataccaa
    16201 atgggaatgc ataagttttc cctttatatt gtgcaccagt aagtgattgt ttagtgtcgt
    16261 ttttagcaat ttcttttgag tattgctctg gaatttcttg gataacacca gattctacta
    16321 attgaccaag ttggtcatgt ggaagtgaga atacatcggc tgccttgctt gggtcttttt
    16381 taacgttttc ttgagcttta gagtcattag actcaatcat ttttacagta acgcctttgt
    16441 tttccttctc gaatttttta acaattgctt tataagacgc ttttgaatct gttgggaccc
    16501 aaagtttgat agttgttttt gaagcagcat caactgagtt agagtgagta gcagcaattg
    16561 ctcctcctgc aactactgaa agagcagcag tactaacaag taattttttc caagtatttt
    16621 tttccatttt ggttctcctc ctagagaata ttttaattac acctatatta tgcaaccgtt
    16681 tgcaaaaaaa gtcaagtctt tttttaaaaa aaataagaaa aataaaaaaa taatatcaat
    16741 taatactaaa tgagaagatt attttagtga gagtattaca ttggcagaga aggcaaacgt
    16801 ttgttttttt cttagattag agtataatag acaaaggact ataccaaaag gtataaaatg
    16861 attagaatga agaggtcgta ggatggtaac tataaaagat gttgctgcca aagctggtgt
    16921 taatccatcg acagtaagtc gtgtactaaa agataatgca tctatttcaa gtaagacgaa
    16981 agagcgcgtg aaaaaagcta tggaagagct gggctatgtt cctaatgttg cagcacaaat
    17041 gttggctagt ggtttaacgc aaaatattgg agttattttt cctccactgg ttttaccaga
    17101 ccgcttaagt gaaccctttt tcatggagat tctttctaca ttaactaatg aagcaaaaga
    17161 gcatgggttt acagtttcta ttgcaacaag tatgtcttct gaggagttgt tagaacaagt
    17221 aaaattaatg catctacaaa aacgagtgga tggttttatt gttctctatt cggatcaaaa
    17281 agatcctatc agagagtatt tattaaaata taaactgccc tttgtggttg ttggtgctgc
    17341 taatggctat gaaaatgaga ttacttatat tgataatgat aatcaactga tggggaaatc
    17401 tgccattgat tatctctata aaaaaggaca taaaagcatc cagtttgtta cagatgattt
    17461 aaattcagaa gtatcagaag agcgctattt agggtatttt aaaggtgcta gaaagttagg
    17521 tttaaaccaa aagccagctc ttttatttga cagaggcaat ccgcaggttt tagaggaatt
    17581 tataaataga gtcaaagaag aagaaacaac tgctttaatt gttattggtg atactgtctc
    17641 tgttagagta atacaatttc tttctttcta taaattaaaa gttccagatg acatttctat
    17701 catgaccttt aataattctc tcttctcaca tctgattcat ccttacctaa gcacatttga
    17761 tattaatgtt aataatctag gaaggacaag cgttcgtcgt ctgattgaca tcattaaatc
    17821 tccagacaaa gtctttagtg agactattat tgtgcctttt actttagaag agcgtgaaag
    17881 tgtccgagat atttctaaaa ataataatta agctatgata taaagacttc tatcaatttt
    17941 cgatagaagt ttttttaaat tatgcttgac ttatgcaatc gcttgcgtta agatggagga
    18001 gtaaagtaag taatcgaaaa ggagaatcaa tatgaaaaaa cgtgcaagtg gtgtcttaat
    18061 gcacatcact tctctaccag gtgatttagg tattggaaca tttggacgag aagcctacgc
    18121 ttttgttgat tttttggtcg aaactgatca aaaattttgg caaattcttc ccttaacaac
    18181 aacaagcttc ggggactctc cttaccagtc tttttctgca gtagctggta atacacattt
    18241 aattgatttt gatttgctaa cgcttgaagg ttttatttcg aaagatgact accaaaatat
    18301 tagttttgga caggatccag aagttgttga ttatgctggt ctgtttgaaa aacgtcgtcc
    18361 agttttagaa aaagcagtta aaaacttctt gaaagaagag agagctacga gaatgctatc
    18421 tgattttttg caagaagaaa aatgggtaac tgattttgct gaatttatgg ctatcaaaga
    18481 acattttggt aataaggcgc ttcaagaatg ggatgacaag gctattatac gccgtgaaga
    18541 agaagcctta gcaggatatc gtcaaaagct tagtgaagtg ataaaatatc atgaagtaac
    18601 gcaatatttc ttttacaaac aatggtttga gttaaaagaa tatgctaatg ataaagggat
    18661 tcaaattatc ggtgatatgc caatctacgt ttctgccgat agtgtagaag tttggacaat
    18721 gcctgaactg tttaaattag atcgcgacaa acaaccactt gctattgctg gtgtaccagc
    18781 ggatgatttt tctgacgatg gtcagctttg gggcaatcct atttacaatt gggattacca
    18841 caaagagtca gattttgact ggtggattta tcgtattcaa tctggtgtta agatgtatga
    18901 ctatttgcgt attgatcatt ttaaaggttt ttcagattat tgggaaattc gaggagatta
    18961 tcaaacagct aatgatggct catggcaacc agctcccggt ccagaattat ttgcaactat
    19021 caaagaaaaa ttaggtgatt tacctattat tgctgagaat ttaggctata ttgatgagag
    19081 agcagagaga ttactggctg gaacagggtt cccaggtatg aaaatcatgg aattcggttt
    19141 ttatgataca acaggaaata gtattgatat tcctcataac tacacagaaa atacaattgc
    19201 atatgctgga actcatgata acgaagttat taatggctgg tttgaaaatc ttacagtcga
    19261 acagaaagct tatgcagaaa attacatgcg tcgtttgcca aatgagccca ttactgagac
    19321 tgtactacgt accttgtatg caaccgttag tcaaacaacg attacatgta tgcaagatct
    19381 actagataaa cctgcagata gccgtatgaa catgccaaat accgttggag gtaactggca
    19441 atggcgtatg agaaaagaag acttaacaga aaatcgcaaa gcattcttaa aagagattac
    19501 aactatttat aatagaggga ataaataagg aatattatca tgacaagaaa ttttacaact
    19561 tacgttggac aacaaggaaa agtattatca gaattaacaa atgaagagat ttacgttgaa
    19621 ttgttaaatt ttgtaaaaga agaagctgcc gctaaatcaa aaaatagctc tcaacgtaag
    19681 gtatactata tttcagctga gtttctaatt ggtaaattac tttcaaataa tttgattaac
    19741 ttagggattt ataaagatgt caaaaaagag ttagaacttg taggtaaatc aattgctgaa
    19801 atagaagatg tagagccaga accatcactt ggtaatggtg gtttagggcg tttagcatca
    19861 tgttttattg actcgatttc ttctttaggt attaatggag aaggtgttgg tttaaactat
    19921 cactgtgggc ttttcaaaca agtatttcgc aacaatcaac aagaagcaga agctaactac
    19981 tggattgaga ataattcttg gttagtacca acagatattt cttatgatgt tcctttccgt
    20041 gactttactt taaaatcacg cctagataga attgatgtat taggttataa gaaagatact
    20101 aaaaattatt tgaacctttt tgatattgat ggacttgatt ataaccttat tgaaaaaggt
    20161 atcacttttg ataagacaga aatcaagaag aatttaacct tgttcttgta tccagatgat
    20221 tcagataaaa atggagaatt attacgtatc tatcagcaat atttcatggt ctcaaatgct
    20281 gctcaattat taattgatga agcgatcgag cgtggttcaa accttcatga tttagctgaa
    20341 tatgcttatg ttcaaattaa tgacactcac ccatctatgg tcatccctga gttaattcgt
    20401 ttattgacag aaaagcatgg ttttgaattt gacgaagctg tgtcagttgt tcgcaatatg
    20461 gttggctata caaaccatac cattcttgca gaagctcttg aaaaatggcc tttagagtat
    20521 cttaatgaag tagtaccgca cctagtaaca atcattaaaa aattagatca aatgattcgt
    20581 gaagagcaaa caaatccaga ggttcaaatt attgatgaag ctggacgtgt tcacatggcg
    20641 catatggata tccacttctc aacaagcgtt aatggtgtag cagcattgca tacagaaatc
    20701 cttaaaaata gtgaattaaa ggttttctat gatatttacc cagataagtt taacaataag
    20761 acaaacggta ttacattccg tcgctggttg gagtttgcta accaagattt agcagattac
    20821 ttgaaagaac taattgggga tagctatctt actgatgcga ctcagctcga aaaattgtta
    20881 acttatgctg atagcaatga agttcatgat aaattagctg ctattaaatt taaaaacaaa
    20941 ctggctctta aacgttacct taaggaaaat aaaggtattg aattggatga atactcaatt
    21001 attgatacac aaatcaaacg attccatgaa tataagcgcc aacaaatgaa tgctttatat
    21061 gttattcata aatacttaga aattaaacgt gggcatttcc catcacgtaa attaacagtt
    21121 atttttggtg gtaaagctgc tccagcctat actattgcac aggatatcat tcacttaatt
    21181 ctctgcttat cagaattaat taataatgat ccagaagtta ataaatacct taatgtccat
    21241 ttagtagaaa attacaatgt gacagtagcg gaaaagttaa tccctgcaac agatatttcg
    21301 gaacaaattt cattggcttc caaagaagca tctggtacag gtaacatgaa atttatgttg
    21361 aatggtgctc ttactcttgg tactatggat ggggcaaatg ttgagatagc tgaattagcc
    21421 ggcaaagaga acatctacac atttggtaaa gactctgaca ctatcattaa tctttatgaa
    21481 acatctggtt atcgttcaaa agattactat gataaagaca aagtaattcg tgaagctgtt
    21541 gattttatca ttagtgatga tatagtgtca ctcggtaatg cagaacgact aaagagattg
    21601 catgatgaat tagtaggtaa agattggttc atgacattga ttgatttgaa agaatacatt
    21661 gctgttaaag aacaagtgct tgctgattat gaagattatg aatcatggaa taagaaagtt
    21721 atacacaata ttgctaaagc agggttcttc tcatcagatc gtacaatcga acaatataat
    21781 caagatattt ggcattcaaa ttaaaaagtc tctaaaagta aggttttcaa ccttactttt
    21841 ttgttgtcta ataaaactat aagtatttat ttttgtccgt agactttaaa aatagaactg
    21901 agcagaactt attctaaata tttaattgca tgtgtattta tattttttat aatataaaaa
    21961 tatgcacctc gtgatacttg gtgatagggt gttttaattt ttgaaatata aactaaaatt
    22021 aaaaaatata atttgatttc aaaatacaga aaaatcagat aaaagcttta taatatagta
    22081 ttatacacac tttaaagagt attatccaca tttaaaaaaa taagataaaa tgataaaaaa
    22141 taaattatta attaattgac agtgtgaatt catagttgta taattaaaca catctaaaaa
    22201 tcggaaaggt aatttgctat gttgaaaaaa ctatttgcac cccctcagaa aagagaacgt
    22261 ttatcagctg aggaaattaa gcataaatat cctcgttatc gtgtacaagt ccttatttct
    22321 atttttgtag gttatatggg atactatttt gttcgtaata cgacttcaat tctatctggt
    22381 atccttaata tgtctgctac tgagattggt attattacct gtgctagcta cattgcttat
    22441 ggtctctcta aatttatttc tggtttgatt tcagatgaat cgaattctaa aattttcctt
    22501 ccagttggtt tgtttcttac aggactagtt aatgtgttaa tcggtgttat tccctctgta
    22561 attactagta tttggctctt tactatcatg tatcttatta atggttggtt acaagggatg
    22621 ggataccctc ctggagcgcg tacattagtc tattggtatg ataataaaga aagaattaaa
    22681 tatgccacca tttggaattt atctcacaat tttggtggag caatagcgcc gattttaact
    22741 ggtgtaggtc tagctcttgc tggtaatgat tctcttaatc aagcgcgtgc tgcttattgg
    22801 tttccagggg tggtggcttg tcttttggct attcttgttt actttttaca agaagatact
    22861 ccagaatcta tcggacttcc gcctatagaa gagtatcata aagagcaata tactaatgtt
    22921 gtggattcaa gtaacatttt agaagagcct gaagttttgg gtatgggcga aattataaaa
    22981 aaatatattc taccaaatac taaactgatg tgggcatcct tgtattcaat atttgtatat
    23041 attttacgtt acggtattgt ctcatggact cctaagtttt tagcgacgag tgtgcaagat
    23101 ggtggaaaag gtattactgc aacagctggt atgggtggtt tttcactttt tgaaataggt
    23161 ggtattattg ggatgctaac agcaggatat ctatctgcca aagtgtttaa aaactctaaa
    23221 cctttaacca atgtagcctt cttagttgta gcaatcttat tactagctgc ctattggttt
    23281 attccagcag gacctcaata tatggcatta gacttcatta ttttactagg tttgggggca
    23341 tcaatctacg gccctgttat gatggttggc ctgtatgcta tggagttggt tccaaaagca
    23401 gcagctggag cagcttcagg attgactggc acatttagtt atgtcggagg agcaacaatc
    23461 gctactctag ctatcggtat tattattgac cactttggtt ggggagtagc atttataata
    23521 tttggtattt ctggatttgc ggctattgtc tgtaccttgc tttcaagaga taagagtcta
    23581 gagtattggt gatattattt gataaaagta tcatttaata tttaatagta gttatgaaaa
    23641 aaggcctctt gaaagagacc ttttttgttt tagataatct atttgttgaa gtatttagtg
    23701 atatcgattg cttttaatag atagtttcta cctagttaaa ataaaactat cccattaata
    23761 atgattcaat tttaatgtca tattcagggt agtgtatgct taaatgttcg ataatcacat
    23821 gataaatttg tgtgattttt gctctttttt ctgggaagtc atgaccaata tagtggaagg
    23881 aacattctat cgttaaaaag agctcatgga ataaggtatt gacttcttct aaaagttcta
    23941 aaagtttttt atcttcagta ataaacgcag gtgcagtatt gatttcatca gcccaagcat
    24001 ctacaggtct aagtggataa gtaccgtatt ctagacataa ttcataagtc ataaaagtcc
    24061 tcctagtctt ttactatttt aacatatgtg tgttcttaag aagaataatt tgtctttaaa
    24121 gcaaagaata tgtcctaagc gtattttttg ctatgttgta aggagtcgtt atataatgaa
    24181 gtatactaac ttattttgca gatgaggaga aaaaatgtca gaaaaaatca gagttttact
    24241 ctattataaa tatgtttcaa ttgaaaatgc ggaagagtac gctgcgaaat atctagaatt
    24301 ttgtaaatct attggactta aaggtcgtat tttgattgcg gatgagggga ttaatggaac
    24361 tgtttctgga gattatgaaa caactcagaa gtatatggat tgggtccaca gcgatgagcg
    24421 ttttgctgat ctttggttca agattgacga ggaaaatcaa caagctttcc gtaaaatgtt
    24481 tgttcgttat aagaaagaga ttgtgcacct tgggttagaa gataataact ttgatagcga
    24541 tattaatcct ttggaaacaa caggtgagta tttaaatcct aaacaattta aagaagcctt
    24601 gcttgatgaa gatactgttg ttttagatac gcgtaatgat tatgagtatg acttaggtca
    24661 cttccgtgga gcaattcgtc ctgatattcg caatttccgt gagttaccac aatgggtacg
    24721 tgacaacaaa gataagttta tggaaaaacg tgttgttgtg tactgtaccg gtggtgttcg
    24781 ttgtgagaaa ttctcgggtt ggatggttcg agaaggtttt aaagatgttg gacaattaca
    24841 cggtggtatt gcaacatatg gaaaagatcc ggaagttcaa ggtgaacttt gggatggtgc
    24901 gatgtatgtt tttgatgatc gtatctcggt gccaattaat catgttaatc caactgtcat
    24961 tagtaaagat tattttgatg gaacaccttg tgaacgttat gtcaattgtg caaatccgtt
    25021 ttgtaacaaa caaatttttg cttcagaaga aaacgaagcg aagtatgttc gagggtgctc
    25081 gcctgaatgt cgtgcacatg aacgtaatcg ctatgtgcaa gaaaatggtt tatcacgtca
    25141 agaatgggca gaacgtttag aagctattgg agaatcctta cctcaagtag caaatgttta
    25201 atattaaaaa atcgctagca ataaagctag cgatttttta gcaatcacaa ttgacatcgc
    25261 aacaagctag gttatcaagg tacatttgat tttctttaaa gaattcaagg gtatcttcgt
    25321 ctaatataat cccttctcgt tgtgcgatat ggttaataat agggacaaag cgttgtctga
    25381 atttataaat atagcagaaa atagtatctc tttgtcgtaa gcttggacca attaagaaac
    25441 agttattaac gatagtagat tcgtcatctt cagtaactat tggcaactga tttttatcgt
    25501 attcaaataa agcgattccg tcaatgagat gacaggtatt tagaaaacct gtggaaagaa
    25561 taggtttatg gaaagactcc gcagtactac cgtcatcaaa agtaacttga tattgttttc
    25621 caatttgttt aatctcaatg gctttcttgc cttcagagat actataatat tcttttttgt
    25681 ggtcttgtat atgttttaaa cgttctttag ttatggggga taaagagatg cttggatcgg
    25741 gattgctctc tttacgacca aacgtatctg tatataattc gacttgattt cccaaatata
    25801 ctagatgtgt tagtgcatca caagcacttt cgtttccacc aatgatgata aatggattgt
    25861 cagatttgat atggaaatta tcaacttgac catagtgcat tcctagatct gcacctttaa
    25921 tatcaatggt atttggattt tgaaattctc ctgttgccat aataagataa tcagcagaaa
    25981 aatctccctt tgaagtttta ataacaaaaa ggctatctcg tttatcaata gatagaacag
    26041 atgtttcgtt ttggataggg agattataat gagcagcaac taattgtaag tagcgtgcat
    26101 attccactcc agaaagatgt tctttttcaa atgagaaagc aggagaagta tctggaataa
    26161 cagcgtttag atctggaaaa ccaaagccgt tggtagtgaa ggaaggtgtg ataaattgtg
    26221 ttgttctggg ccatcttaaa aaagattctc caatgtgccc tttttcaatg ataagaaaat
    26281 ttgtaagtcc taatcgttgc atggcagaac caaaaccgat tcctgcagcg cctgcaccga
    26341 taattatggt ttgataatgt ttcatgtctt ctccttgaca aaaataaatg tgtaagataa
    26401 aatacttaac cagttaataa taatacagtt taacaaataa aagagtgttt gtaaagaaag
    26461 gactatttgt gtttttacta ttatgtattt tatcgatgtg gctcatgatt tttggggttg
    26521 cattctatta ctttgggtcg ctacatcaat cattgacttc aaggattatt tatcagtttg
    26581 tacttactgt tttgataacg acaatagctt ggttcatggg tgcctatttt ttaggatttg
    26641 aaggtcattt taaaactgtc tttcaattcc aagaagcaga tggtaaacaa attgtaaatt
    26701 gtttatttca actttgtttc gccctttacg cagtagtaat gcttattggg tcaattattg
    26761 atagagttca gacgaagcga cttttattag ctgttgtttc ttggcttttc ttggtgtata
    26821 cgcccttagc ttatttaatt tggaattcag agggtgtttt tgcaaagatg ggagtacttg
    26881 atttttcagg cggaatggtc gttcatttat ctgcaggact ttcttcgtat attttagctc
    26941 atgttattgg caagagtgaa catcagcaca ataagattaa aaatgataat ctctttttag
    27001 gaatgatact tattacattt ggttggtttg gatttaatat gggtccagtt ggtgagtgga
    27061 attcacaagc tattatgata cttttaaata ctatatttgc tattataggt ggtggtttgg
    27121 cgtggacatt ggctgctaag tggaatggtg aggaagagaa aacaggaagc ttattgaatg
    27181 gcattattgt tggtcttgtg acgagtacag caggagttgg gtatctctta acttggcaat
    27241 tattagctgt aacattcttt gcatccctct tcacctactt tgtaacggat tacgttgcga
    27301 aagcttttgc cattgatgat gtcgtttcct cctttggaat gaatggtatt gggggattgt
    27361 tgggaagttt aggagttggt ctctttaaat tatcacatat gccagttcaa ctgttagcat
    27421 tagctaccac aattcttttg tcaattataa tgacctatat catatctaaa gcgattttta
    27481 gaaaataata aataaggttg agatgtacat ctcaacctta tttattattt tagcaaggag
    27541 agtgactaaa tagaattatt atcattttct tactagacag caactgtaca aggttctgaa
    27601 agttagttga ttattaatgg tagttagcat tttttaagaa ttggagtact tttaaaaggt
    27661 gattgtttta attagttata gttgttatta aaaatcgtct aacagtaagc atagttatca
    27721 atgtaattta ctgaattttt attcaaaaaa gcttgactta tgtataaaga tgatttatac
    27781 ttgttcctat cataataaaa ggaagatagt ttatgtcaaa aaaaataata ttaggaattt
    27841 tatctctttt atctgtcgtt actttggtgg cgtgtggttc atcagacaaa cagctacaag
    27901 ataaagttga gaaaaaaggg aagttagttt tagcggtgag tccagattat gctccctttg
    27961 agtttaaagc gcttgttaat gggaaagata aagttgtcgg tgcagatatt gagttagctc
    28021 aagctattgc tgatgagctt catgtcaaac tagaggtatc tcctatgagt tttgacaatg
    28081 ttttatcaag tttacaaaca ggtaaggctg atatggctat ttctggatta tcatatacta
    28141 aagagcgtgc taaagtctat gatttttcaa aaccttatta cactacagaa aatgcggttc
    28201 ttgttaggaa gtctgattta ggaaaatata cttcaatcga aagccttaaa ggtaaaaaaa
    28261 tagcagtaca aaaaggaagt attgaagagg gagtttctaa gaaatcgttc aaaaattctc
    28321 acgtcgtgtc actgacagcg atgggagaag cgattagtga gttaaaatca ggcaaggtag
    28381 aagccgttga tttagaaaaa cccgtagctg aaggttactt ggcccagaat ccagaccttg
    28441 ttcttgctaa gtttgccctt aaaacaggag aaggggatgc taaggctgta gctctaccta
    28501 aagacagtgg aaaaatgttg aagactgtta acaaagtgat aaagagactt gaaaaagaag
    28561 ataaatataa agtttatatt tcagatgcgg ctaaattaac tggcaatcaa gttgaataat
    28621 attaaaaagc ctagtcatag gcttttttgg atgttataat atggcttgac taagaataat
    28681 agcataaagg cagaggaata tatggttcag ggttcaatta atattgaaaa atataaagat
    28741 ttggcgctcc aaaaacaaag cgagcatcgt aaatttttag caagtttaaa aaagaaagct
    28801 cctaaaaact tagatagaat agtccaacaa atacatcaag aggtttttca agaaattgat
    28861 tgtacagcat gtgctaattg ttgtaaaact ttgggtcctc tatttacaga agctgatatt
    28921 tctaggattt ctaaacattt ccggatgaag ttatcggctt ttgaagattt atttttagga
    28981 gtagatgaag atggagataa agtatttaaa gcaatgcctt gtccgttttt aggcgaggat
    29041 aacttatgta gtatatacga tgtcagacct aaagcttgtc gtgaatttcc acatactgat
    29101 cgtaagaaaa tctatcaaat taataactta acgataaaaa atactcttac ttgtccagcg
    29161 gcataccttt ttgtggaaaa attgaaatca cgtataggga aataataacc cagagaaagt
    29221 atcataaaaa tattgacaat ctgcttttat ttgggtatcc tagtaagtga acgtgaaaat
    29281 gttacaatat cttgaggagg tgaaacatat gtcaaaaaca gtagtacgta aaaacgaatc
    29341 acttgacgat gcacttcgtc gtttcaaacg ttctgtgact aaagctggta ctcttcaaga
    29401 atcacgtaaa cgtgaattct acgaaaaacc ttctgtaaaa cgtaaacgta aatcagaagc
    29461 agctcgcaaa cgtaaaaaat tctaattaga tagaatatga aaaaacagtt cagaattgag
    29521 ctgttttttc atatattata actatgagtt aataaaaaaa ctcggtaatc cgagtctttt
    29581 attatttgtt agcgagtaag tcgcggattt cagcaagtaa ttgctcttgt gttggttcaa
    29641 caacttcaat aatttcttct tcagcaggtt tcttagccat aaccttgtta gcagctttaa
    29701 cgatgaagaa taaagtagta ccaacgatta agaaattaat aactgcactt aagaaattac
    29761 cgtaagcaac accgttccat gaaagctgcg caatatttga aacgccagca gcttttaata
    29821 ctggattaag gataagcggt gtaataacgt ctttaacaag tgaagtgata atagcattaa
    29881 aagctgcacc taaaataaca gcaacagcaa ggtctaaaac attaccctta aataaaaatt
    29941 cttttagttc tttaatcatg tgaacaagct ccttaatatt atttaattca cgttcattat
    30001 agcagatttc ttttgagaaa tccaaataat aggtttcaaa ttatctgtaa atttttatat
    30061 taaattagtg aagaagaaat ccttgaaaaa gcttttgaaa aaatttacac agaaaagaaa
    30121 aaatgatata atcaaatgta aaactataat gggttatttc tgtggaggtc atgacttggc
    30181 aattgataaa gaaaaaattt ctgaaatcaa gaacagtgtt aacattgttg atgtcatcgg
    30241 tgaagttgtc ggtttgacaa aaacaggacg caatcatctt ggtttatgcc catttcataa
    30301 agaaaaaacg ccatctttta atgttatcga agataggcag ttttttcatt gttttggttg
    30361 tggtcgctct ggtgatgtct ttaaatttgt ggaagattac caacatatta gttttttaga
    30421 tagtgtacaa gttctagctg aacgttcagg aatacctcta gatactaatt ttaaggggca
    30481 ggttcctaaa aaacctaaag ctaatcagtc gcttttagat attcatagag ttgcttcggg
    30541 attttaccat gcctatttaa tgacaactaa tgatggtgaa cgcgctagac aataccttgc
    30601 tgagcgcgga gtgacagagg acttaattaa acattttcaa ataggattat cgccgggagg
    30661 gcaggatttt ctatatcgtc gtctggcaaa agagtttgat gaaaaaactt tgatgtcttc
    30721 tggtttgttc aattattcag aaaatagcaa tcagttttac gattcattta ataatagaat
    30781 catgtttccg ttgactaatg atatcggtga agtcattgcg ttttcaggac gagtttggac
    30841 ccaagaggac attgatagaa aacaagctaa atataaaaat tctcgtgcta cccctatttt
    30901 taataagtct tacgagcttt accatttaga taaagcgcgt gctgtaatta ataaagcaca
    30961 tgaagtttac ctaatggaag gctttatgga tgttattgca gcttatcgtg caggaattga
    31021 gaatgttgtt gcttctatgg ggaccgcttt aaccaatgaa cacgttagac accttaaaag
    31081 atttactaag aaagttgttc tgacttatga tggtgataga gctggtcaaa acgcaattga
    31141 caagtcttta gaactgctat cagatatgac tgttgatatc gttaggatac ctaataaaat
    31201 ggaccctgat gaatttctac aagcaaattc agcagaagat tttaaacaac tcttagaaaa
    31261 agggcgcatc agtaatacag agttttatat ccactactta aagcctgaaa acaccgataa
    31321 tttacaatct gaaattgctt atgttgaaaa aatagcgaaa ttaattgcta aatctccatc
    31381 aataacagca caaaattcat atattacaaa agtggctgaa ctcttgccag attttgatta
    31441 ttttcaagta gaacaatcgg ttaataatga acgattgcat catagaagtc aacagcaagc
    31501 ttcaccttca gtacaaacaa gtgctacagt gcaattacca cagacaggta agttatcagc
    31561 tatcactaaa acagaaatgc aattgtttca tagattattg aatcatccct acctgttaaa
    31621 tgaattccgt aatagggata atttttattt tgatacaaca gaaatacaag ttttatatga
    31681 gttgctaaaa gaaagtggtg agatcacgtc ttatgattta tcacaagaaa gtgacaaagt
    31741 taatcgtact tattatatga ttttagagga acaattacct gtagaagtat ctattggaga
    31801 gattgaagcc gtcgaaaagg cacgtgaccg tttattgaaa gaacgtgatt taagaaaaca
    31861 aagccagtta attagacaat ctagtaatca aggtgatcaa gaaggagcct tggctgcatt
    31921 agaaaattta attgctcaaa aaagaaatat ggaatgagga acctatggca gagaaaaaag
    31981 gaaatacaac ttttaatgtt caagttgcgg attttattcg taatcataaa aaacaaggta
    32041 ctgcaattga tgatgaggta acagaaaaac ttgttattcc ttttgtttta gatgcagatc
    32101 aaattgatga tttgttagaa cgattgacag acggtggaat ttcaattaca gacaaagaag
    32161 gcaatccttc aacaaagtat gtggtagaag ggccaaaacc tgaagaattg actgatgaag
    32221 agctcatagg aagtaattct gctaaagtta atgacccagt acgtatgtac ttaaaggaaa
    32281 ttggtgttgt tcctctttta acgaatgaag aagaaaaaga attggctgtt gcggttgccg
    32341 agggagattt aatggctaaa caacgtttag ctgaagcaaa ccttcgtctt gttgtctcaa
    32401 ttgcaaaacg ttatgttgga cgtggaatgc aatttcttga tttaattcaa gagggtaaca
    32461 tggggctcat gaaagctgtt gataaatttg attattcaaa gggattcaaa ttttcaactt
    32521 atgcaacatg gtggattcgt caagctataa cacgtgcaat tgcagatcaa gcgcgtacga
    32581 ttcgtattcc tgttcatatg gttgaaacga ttaataaatt ggtccgtgaa caacgtaatc
    32641 ttcttcaaga acttggtcaa gatccaactc ctgagcaaat cgcagaacgc atggatatga
    32701 caccagataa agtacgtgaa atcctaaaaa ttgctcaaga gccggtatcg ctagaaacgc
    32761 caataggtga ggaagatgat agccatttgg gtgatttcat tgaagacgaa gttattgaaa
    32821 atccagtaga ttatactaca cgtgttgttc ttcgtgagca attagatgag gttttagata
    32881 ctcttactga ccgtgaagag aatgttcttc gtttacgctt tggtcttgac gatggtaaaa
    32941 tgcgtacgtt agaggacgta ggtaaagtat ttaatgttac ccgtgagcgc attcgtcaga
    33001 ttgaagctaa agcccttaga aaacttcgcc atcctagtcg tagcaagcaa cttaaagatt
    33061 tcatggaaga ttgattgctt ctcgataatc gtttttagaa taggaagttt agtttctaat
    33121 aaaaatgatt tgcaaaggag ttatcttaaa gacggtatta atattttcag agaggtatta
    33181 taatgtcaga agtaaaacga tatagcgaag aagaagttgg aaaaataaaa gatcgtatcc
    33241 tagaagcttt ggagatggtt atcgatcctg aattgggaat agatattgtt aaccttgggt
    33301 taatttatga gattcgcttt gaagataacg gtcgaactga aattgatatg actttaacga
    33361 ccatgggatg cccgttagca gatttattga cagatcaaat ccatgatgtt atgaaaacag
    33421 tcccagaggt aactgaaact gaggtgaaat tggtatggta cccagcctgg tctgttgata
    33481 agatgagtcg ttatgctcgt atcgctttgg gaattcgttc ataagataaa gagtaaaggg
    33541 gtgaaatatt ccctttttta gtgtacaata aacgaagaat taaaagttaa acttattttt
    33601 aaaggagcaa ggaatgattt taattacagg ggcaaacggg caattaggta gtgaattacg
    33661 tcacctttta gacgaacgaa ctcaagagta tgttgctgtt gatgtagctg aaatggatat
    33721 tacaaatgcc gagatggtgg acaaagtctt tgaagaggtg aagccatctt tagtttacca
    33781 ttgtgcagct tacacagctg ttgatgcggc agaagatgaa ggaaaagagc tagactttgc
    33841 aatcaatgtc actggtacag agaatgtcgc taaggctgct gctaaacatg atgcaacact
    33901 tgtttacatt tcgacggact atgtgtttga tggtgaaaaa ccagtggggc aagaatggga
    33961 agtagatgat ctacctgatc ccaaaacaga gtacggtcgt acaaaacgta tgggtgaaga
    34021 gttagttgaa aaatatgctt caaaattcta taccattcgc acagcgtggg tatttggtaa
    34081 ctatggtaaa aactttgtct ttacgatgca aaaccttgca aaaacacaca aaactctaac
    34141 tgttgttaac gaccaacatg gacgaccaac atggacacgt actttggctg agtttatgac
    34201 ttatttagct gaaaatcaaa aagattttgg ttactatcat ttatcaaatg atgctaaaga
    34261 agatacaaca tggtatgatt ttgcggttga aatcttaaaa gacacagatg tggaggtaaa
    34321 gcctgttgat tctagtcaat tcccagcaaa agcaaaacgt cctttaaatt caactatgag
    34381 tttggaaaaa gcgaaagcaa caggatttgt gattcctact tggcaagatg ctttaaaaga
    34441 gttctacaag caggaagtta aaaaataaga tcaaaacaaa gagtagtaag gctagagaat
    34501 ccttttagct tactgctctt tttagctaac ttttctaatt tatggtataa ttgtatggat
    34561 tgtttagcta gaatggagaa gatgatgcaa gatgttttca ttataggaag tagagggttg
    34621 ccagctcgtt acggtggttt tgaaactttc gtttcagaat tgattaatca tcaaaaaagt
    34681 tccgacataa aataccatgt tgcatgcctt agtgataaag aacatcatac tcattttaac
    34741 tttgctgacg ctgattgttt tactataaat cctccccaat tagggccagc acgcgtgatt
    34801 gcttatgata ttatggccat taattatgcc cttgacttgg ttaagacaca tgatttaaaa
    34861 gagcctattt tttatatttt aggaaataca attggtgcat ttatttggca ttttgccaat
    34921 aaaatacata aagtcggtgg cttattgtat gttaatccgg atggtttaga gtggaagcga
    34981 tcaaagtggt ctcgtcccat acagcgttat ttaaaatacg ccgaaaaatg tatgactaaa
    35041 aatgcagacc taattatttc tgataatatt ggtattgaaa attacattca aactacctac
    35101 tctaatgtga agacaaggtt cattgcttac ggtacagaga ttaattctag gaaattatcg
    35161 tcagatgatc cacgtgtcaa acagttgttt aaaaaatgga atattaagtc taagggttac
    35221 tatctaatcg ttggtcgatt tgtccctgaa aacaattatg aaacggctat tagggagttc
    35281 atggcttcag atactaagcg tgatttagtt attatctgta accatcaaaa taacccctac
    35341 tttgaaaagt tgtccttaaa gacaaacctt caacaagata aaagggttaa gtttgtaggt
    35401 acgctctatg aaaaagattt gctggattat gttcgtcaaa aagcctttgc ttatattcat
    35461 gggcatgaag ttgggggtac taatccagga ctgcttgagg ctttagctaa tactgatttg
    35521 aatcttgttc tagatgttga tttcaacaaa tcagtagcag gtctttcaag tttttactgg
    35581 actaaaaaag aggggaattt agctaagctt attaacgata gtgatcaaca acaggattta
    35641 tctacttacg gtgatcgagc aaaagccatt attcaagaaa actacacttg gaaaaaaatt
    35701 gttgaagaat atgaggattt gtttttaaat gaaagttaat attttaatgg ccacctacaa
    35761 tggagagaag tttctggcgc agcaaatcga aagtatccaa aagcaaacct ttaaagagtg
    35821 gaacctccta atcagagatg atggttcaag tgacaagaca tgtgatatta ttagaaactt
    35881 tactgctaaa gattctcgaa ttcgtttcat taatgaaaat gagcaccata atttaggagt
    35941 tattaaatca ttttttacgc tagttaatta cgaagttgca gatttctatt tttttagtga
    36001 tcaagatgat gtatggttgc cagaaaagtt atctgtaagt ttagaggcag caaagcataa
    36061 agcatctgat gtaccattac ttgtttatac tgatttgaaa gttgttaatc aagaattaaa
    36121 tattttacaa gacagcatga ttcgggctca atcgcaccat gccaatacga cattattacc
    36181 agaactaact gagaatactg ttactggtgg gactatgatg ataaatcatg cattagctga
    36241 gaagtggttt actcctaatg atatcttaat gcatgattgg tttttagcac tcttagcagc
    36301 aagtttagga gaaatcattt atttggattt accaactcaa ctataccgtc aacatgacaa
    36361 taatgttttg ggagcgagga caatggataa gaggtttaag atacttcgtg aaggtcctaa
    36421 atctatattt acaaggtatt ggaaacttat ccatgactca caaaagcaag cttccttaat
    36481 tgttgataag tatggtgata ttatgacagt tactgatctt gagctcatta aatgttttat
    36541 aaaaattgac aaacagcctt ttatgacaag attaaggtgg ttatggaaat atggttatag
    36601 caaaaatcaa tttaagcatc aagttgtgtt taaatggttg attgcaacaa attattacaa
    36661 taagagataa gatgatgaaa aaaattttac tagtgatacc tgcttataat gaagaaggta
    36721 gtattgcaaa gacagtccag acgattgtag attttaaggc gtctcgctcg ctaccttttg
    36781 agctggatta tattgttatt aacgatgggt caaccgatgg tactccagag ttattagata
    36841 gattgggttt aaatcatatt gatcttgttc aaaatctagg tattggtggc tgtgtgcaga
    36901 caggatactt atacgctaat cgtaatcatt acgatgttgc cgttcaattt gatggtgatg
    36961 gtcaacatga tattagaagc attgaagatg ttgttatgcc aatcttaaat gacgaggcag
    37021 attttgttat tggttctcgc tttgttgata aaaaacatca aaattttcaa tcgacagcaa
    37081 tgaggcgtct tggcataaat ttaatatctg ctgctataaa attaacgacg ggacacaaag
    37141 tctatgatac aacaagtggt tatagggcag ctaacgcggc gctaatagct tatttatcgt
    37201 gtcattatcc agtacaatac ccagagcctg aatcaactgc tcggattttg aaaaaaggct
    37261 atcgtttgaa ggaagtcaca gctaatatgt ttgaacgtga ggcaggtact tcaagtataa
    37321 gttccctaaa atcgattttt tatatgacgg atgttttaac gtcaattatt attgcagggt
    37381 ttattaagga ggatgataaa tgagtttagc attacaaatc gaaatgttta ttttagccat
    37441 tgtcctactt tatatgatta taagaatggt taataaaaac acattttctg ttaaacgttc
    37501 tttaccttgg ctattagttg gtttaattct agtctttatt agtgtatttc ctggggtggt
    37561 ctcatttatc gctcatcacc ttggattcgc tttaacgatg aatttcttgt tatttggcgc
    37621 tattttattt ctgttttgtt tagaattatt tgatactagc agcaatacca aaagagatga
    37681 gcaaattaaa cgcctgattc aagaggtttc tttgttgaaa aaagagatag aggatagcac
    37741 tttagatgag taaaaaacat atttttattt gtctgttttt catgcttttt ttagtaggtt
    37801 gccaaaataa taaaaaagta aaaacagata aaaaagtaga tattacagct tattattatc
    37861 ataataatac cccgcaatac agtgttcaga ttaatgctat tcatttacaa acagcattgg
    37921 ataaagtgag gacaggggag tttgagagca aagactttac acactatact tttgatagta
    37981 tcaagcggaa gtaccaagtt agtggtaaaa aagtccttat ggataataac tatagatttg
    38041 gttctggtat aaaaaatact agacaggatg ctattgctat tgtaaggtta ctgcttgaaa
    38101 agaataaaga ttatttaatt tacatgaatg gcttagaaga gccaagtgtt ttatataatc
    38161 cagaaaataa acgttataag ttcacaaata acaaaggaaa agcaattggt aatattccag
    38221 ttggtttaac tgcttttgaa aacagtgcag aaactcaaga atatgtgttg aaaaatattc
    38281 agaaaaacga aacgatttat cttggtaata ccagagtaga taatcctcga gtaactgtta
    38341 ataacaaaaa aagagatact ataggagttg aatacggtaa acgtgttact tataggattc
    38401 caatttattc aaagcagctg actgtcagag ttagccctaa ttttgtagta gatagtacta
    38461 attacaatta ccgactttct caagcgccta ttattgcgga agaaggtaaa gagggacgtt
    38521 tgtatccaat agatagcagt attagtgtcg atggtgataa aattgtttta aatagtagtg
    38581 gtgatgaaac agagcaaaaa gaattattac aaaatagcct gtataagctt cactctattg
    38641 ataagttaga ttttgatttg acaaatgcta atattaaaga gttagagatt acaggacatg
    38701 ttgcttcaaa aagagattat aatttgtctg ttgctcagga aaagaaaggc gtcatagaga
    38761 ctaaacggag tctaatttct gttgataatc aaaagcaaga tcaagggatt tttgtcgagg
    38821 attctaaagg ctatataaag acacctagtt tgagcagtta tggtataaat tttgccatgt
    38881 atgacgacaa tcaaaataga ttattgcaag gttcagagta tattttagga agattgagtg
    38941 cagataaaaa agtatttcta taccaaccta atggctcttg gataaatact caaaaaacga
    39001 ttaagagttt aaataaagaa tatcttaacc aaaatgctta ccatattaga ggaaataaca
    39061 ttataggtat tgatggttat tcaaatgaca tagacttgaa tcctaggtta tgggcttata
    39121 actataaaaa acaagtgaaa agtaaccaat cattatttaa attacgaggt ttgtctaata
    39181 agtataagta tttcttaata cagacaaaat ctccaaaagg ctacttacaa agcaataaac
    39241 cattttattt tacagtaaat aaagatagtg tttctaaagc acagtttggt aactataata
    39301 taaatggtta tataatggac atgcagtaca acaaacaaga atacaatgca cttgcaaaca
    39361 ctccaaaagg tcaaaaaaca aagaaaactt ttaaacccat gaccctagtg attctttttg
    39421 cggtagcttt atttattttc tatattacag ctataagatt tgtatttaaa agaatgtgag
    39481 ttaactgtta gacagaggta attatgaaag aaatgacggt ctcagaaata agagaagttc
    39541 aacttgaaat gttagcttat attgataaag ttgcgcgtga taataaaatt gaatattctt
    39601 taggcggagg cagcctactg ggagcaatga gacataaagg attcattcca tgggatgacg
    39661 atatcgattt gatgctagaa agaagccagt atgagcgttt gatgaaagca ttagctgatg
    39721 ctaataatag cgattttaaa ttgctacatc attctgttga aaaaaatcta tggccatttg
    39781 ccaagctgta tcacacaaag tcaatgtact taagtaaaac agatagaatt catccttgga
    39841 cagggatttt tattgatatc ttcccacttg accgattacc agagagtgca gaagaacgtc
    39901 aaagattctt taaaaaagtt cactctgctg ctgcaaattt gatgtgtacg acttacccca
    39961 attttgctag cggttcccga aagctttatg caaatgcaag gctaattttg gggctacccc
    40021 gattcatcgc ttatcacggc caagctaaaa aacgtgctga aattgttgat caagtgatgg
    40081 aaacatataa taatcaagag gttccttaca tgggttatac cgattcaaga tatcgcttga
    40141 aagaatattt tccaagagaa attttttctg agtatgaaga tgttatgttt gaaaacatta
    40201 aaactcgaaa aattaaaaat gagcatgctt atctgaatca attatatggg ggtagttata
    40261 tggaattacc tccagaaagt aagagagaaa gtcatagtta ttacacttgg tactggaaag
    40321 aaggttaatg atgaatattg gagttatttt tgcaggtggt gttggtcgtc gcatgaatac
    40381 caaaggtaaa ccaaagcaat tcctagaagt gcatggtaaa cccattattg tgcatacaat
    40441 tgatattttc caaaatacag aagctattga tgctgtagta gttgtctgtg tatcagactg
    40501 gctagattat atgaataatc tggtagagcg tttcaacctt acgaaagtaa aagcagttgt
    40561 tgcaggtgga gaaactggcc aaatgtcaat ttttaagggg cttgaagccg ctgaacagct
    40621 ggctacagat gatgccgttg ttttaattca tgacggagta cgtccattga ttaatgaaga
    40681 agtcattaat gctaatatac agtcagtaaa agaaacagga agtgcggtaa cttcagttcg
    40741 tgctaaagag acagttgttc tagtcaatga ttcttctaaa atatctgaag ttgttgatcg
    40801 tactcgttcc ttcattgcta aagcgccaca aagtttttat ctatcagata tcctttctat
    40861 tgaacgtgat gccattagca aagggattac tgatgcaatt gattctagta ctttgatggg
    40921 tatgtacaat cgtgaactca ctattgttga gggaccatat gaaaatatca agataacaac
    40981 accagatgat ttttatatgt ttaaagcttt gtatgatgct agagaaaatg aacaaattta
    41041 tgggatgtaa tgatgattaa atttagtaat aaaattgtac aagaagattt agcacttatt
    41101 agtgaccaaa attatattaa ttgggaaaga cttgctaata aagccgtttt aataagtggt
    41161 tctaatagta tgttagcgag ttatatggta tttttactag cctatctcaa tgagacaaga
    41221 aattaccaaa cgcaaattat tgcgacagct agaaacattg aaaaagcccg tgataaattt
    41281 tcagatttag ttgggaaaga ttatttcacc ttaattcctt atgatgtaga agaacgccta
    41341 gagtatgatg gaaaagtgga ttacattatc catgcagcaa gcaatgctag tccaactgct
    41401 attttgagta atcctgttag catcataaaa gctaatacaa ttggtacatt gaatttatta
    41461 gactttgcaa aggaaaaaac aattgaaaac ttcttgttct tatcaacgcg tgaagtatat
    41521 ggaacatcta ttaaagaagt gattgatgaa gaagcttatg gtggatttga tattttagct
    41581 actagagctt gttacccaga aagcaaacgc atggctgaaa ctctactgca aagttactat
    41641 gatcaatata aggtaccctt tactattgct cgtattgcac attcatttgg tcctggtatg
    41701 gagctcggta acgatggtcg tattatgaat gatcttttat cgaatgtcat tgatggcaaa
    41761 gatatcgttt taaaaagtag tggtacagca gagcgtgctt tctgttattt agcagatgct
    41821 gtatcagggt tatttactat tttattaaat ggtgaagttg gtcaagctta caatgtagca
    41881 aatgaagatc aacccattat gattaaggat ttggcacaaa agttagttga tctcttttct
    41941 gataaaaata tttcagtagt atttgatatt cctaaaacta tgagtgcagg ttatagcaaa
    42001 atggggagaa cacgtctcac tatggccaaa ttagaagcgt tgggatggaa gcgagaagtt
    42061 tctcttgaat ctggaatatt aaaaacagta caagcatttg aggagtaata tggtaaagac
    42121 agcagtttta atggcgacat acaatggcga aaaatttata tctgaacaac ttgattcaat
    42181 tcgccaacag acattaaaac cagattatgt attattgagg gatgattgtt caacggatga
    42241 aacagtcaat gtcgtcaata actatatcgc aaaatatggg ttagaaggct ggaaaattgt
    42301 taaaaacgac aaaaacttag gctggcgttt aaattttcgt caattactta ttgatgtgtt
    42361 agcctatgag gttgactatg tcttttttag tgatcaagat gatatttggt atcttgataa
    42421 aaacgaacga cagtttgcca ttatgtcaga taatcctcaa attgaggttt tgagtgcaga
    42481 cgtcgatatc aaaacgatgt ctacagaagc cagtgttcca cattttctaa ctttttcttc
    42541 tagtgataga atcagtcagt atcctaaagt atatgattat caaactttcc gtcctggatg
    42601 gaccattgct atgaagagag attttgcgca agctatcgct gataattgga cttttggagc
    42661 taatatttcg cacgataacc ttatcgagac aactgccagt attattggaa atggttataa
    42721 ccttaacgaa agcgtaggag ttcatattcg atatggagga aacgcaagtg gtaatagcaa
    42781 atttgttttg aattggaagt cgcctaaaac acaacatatc acaggattaa aaacatttgt
    42841 tgatttttac gaaaccatgc agaaggttgt caacaaatat caaaatgatg tcgctttaaa
    42901 tcaacgctta gcctttgaaa aagaaagatt ggaagcttct aaattgtcat taattcataa
    42961 tagtgctttt gtgattaaaa attggcgact ctataactct aatagtggac gcatgagaga
    43021 tttattattt gcgtttaaaa agtaaggaat aatatggtaa aaacagcagt tttgatggca
    43081 acatataatg gtcaaggttt catccatgat cagctagatt caattagaaa tcaaacgttg
    43141 agaccagact atgtcttaat gagggacgat ggctctactg atgatactgt aaaggtggtt
    43201 gaagactata taaaagagca ccgtttagat ggttggtcaa taactagtaa tgataaaaat
    43261 ttaggttggc gtttgaattt tcgtcagttg cttttagacg ctaagcgtct ttctgtagat
    43321 tatttatttt ttagtgatca agatgacaca tggtatcatc ataaaaataa gatgcaagtt
    43381 gatattatgg aagaacgcca agatataaac cttcttagtg ccgatattga tatcaaaaag
    43441 ttatcagaag aggctactat tcccaataat tttaagttca atggtgagga aacaatttct
    43501 aagtatccat tagacttttc atatcataac tatcgtcaag gttggacttt ttgtttacgc
    43561 aagtcgtttg ttgatcaagt aatgactttt tacagtgata atttaatttt gtcacatgat
    43621 aatctttttg cagggatatc tggtgtcatt ggttcaggtt acaatttaaa cagaagtgtt
    43681 ggtgaacata agcgacatgc tgagaatgca agtgggaata tcttgaatat gagctcaagt
    43741 tacgaaaggc atttacaaga actgtatttt gttttgagtt actatatcat ccttgtacca
    43801 tttttagaaa aaagtagctc tagtcaatta agggaagcta aagcttatct ggattttaat
    43861 cagcaacgtt ataaaaatgc tcaaaataaa gattttgtag caatacttaa acaaatgatg
    43921 actaaaaaac ggttctatga tagcttttca aatcgcattc gtgacttggt gtttttatta
    43981 aaagatataa aaaggagtta atatgaacct caaaaaagtt cgtctatttg aaaagttttt
    44041 tctagacaag aacttgcata aaacatattt tatattaggc ttaacgatta ctattgtatt
    44101 gagcctatta atgccatttt tcaatgaacc tgatggacaa taccatttag ctgtgtctgg
    44161 tcgtatttca aataacatta ttgatacttc ttactatgga gaatataata ttgattcagg
    44221 tatgagacga caatcgacct cctatagaga tggtactaga tttgaaaaat actatttaaa
    44281 taaagccaat tttgttgatg gtaatcattt gcctcgtgag ataaactatt ctaaacataa
    44341 ctttgttttt tggggtcatc ttgtgccagc agttggctta tttttaggca aatttgtgta
    44401 tccttctatg ggggtaatga ttacagtagc aaggttattt agttcctttg tctcaatcac
    44461 cttggttgcc ctcattatcc ggtatgtgaa aaaaggaaag ttaatctact ttgctatttt
    44521 cctcagtccc gtagttctaa attcatttgc tagtctatct tatgactcga ctggatatat
    44581 tgtatttgga gcatttttat cgcttttgat aaatctttta gttgataaat cgttgactaa
    44641 aaatcttagt attgctctta taggattgat tcttttagtc attgttgcat gtaagcagaa
    44701 ttactggctg tttttattgc tagtaatacc ggtattattg caggttaaca atcctaaatt
    44761 aatgggctat aatcagaagc tatcacattt atttcaaggt cttaaatcta aaaagctatt
    44821 ttggctatgt ctaacctttt tgtcacttgc tatagtttat gttctgaccg caagacatgg
    44881 tggtgtcctt gctgttttaa gacgttatgt aatgacattt ggctataatt atgctggtat
    44941 caatatttta agtaatgaca ttacttcctg gcttgcctct ccatatccaa gtcagaattt
    45001 tattcccact tgggtatcat cagtatggta tctcatggtc tttctagtat ggttttctga
    45061 agaaaaattt gttaagtcaa aaaatatggg atttcttttc ttagctagtt tctttgtggg
    45121 aattcttggt gtctttttta tcatgttaga atatcgtgga gcgccaacaa gctacattga
    45181 aggagttcaa ggacgttact ttactgttac attgcttttg gtacaagttt ttgcatcaag
    45241 tattccagcg cgtctaaatg aggcaggaag gaaagtggtg ccgtatgcgt taactggctt
    45301 ggtaattgta tcaaatgcct tattgttatt tgatactgtt attagtttaa ttatgaggag
    45361 aaactaatga aacttttgaa aaatatgttt tataatacct cataccagtt attgactttg
    45421 ttgttacctt tagtaacagt tccttatgtt tcccgtgtac tatctcctca aggcatcggt
    45481 attaatgcct atacgagctc cattgtcatg tactttactt tatttggtgc tttagggata
    45541 tctttgtacg gtaatcgtga gatagctttt gtgcagtcaa ataagtataa gagaagtaag
    45601 atattttggg aattagttgt attaaaactt gcctctgtat ctatagcaac gttattattc
    45661 tttggatttg tcttactgac aaacgaatgg caattattct atttaattca aggtattaat
    45721 ttattagcga ctgcaactga tatttcgtgg tattttattg gagttgaaga ctttaagata
    45781 attgtcattc gtaataccat tgttaagctt attacagttg ttttgacctt cttagttgtg
    45841 aagacgccag atgacctagc actttatatg tttttaattg catttgcatc attactcgga
    45901 aatttaacag tctggcatca tttaaagcat gaaatcatca agataccatt tagtagatta
    45961 gatgtcttag tacatctaag accaacttta atgttatttt taccccaaat caccatgcag
    46021 atttatctct ctcttaacaa gagtatgctg ggagcaatgg atagtgtggt cagtgctgga
    46081 tattttgatc aatctgataa aattattcgt attttattta ccattgtttc agcaatcgga
    46141 ggagttttct tgccaaggct ctccagtcta ttttcttcag gaaaagaaaa gcaagccaag
    46201 gctttacttt tgaaacttgt tgatttaagc aatgctatct caatgttaat gattgcggga
    46261 gtggtaggag tttcgtcgac atttgctgtc ttcttttttg ggaaagggta tgaagttgtc
    46321 ggtccgctaa tggctgttga gtctttgatg atcatttgca tttcttatgg taacgcttta
    46381 gggacacagt atctactggc ttcaagacgt acaaaagctt acacaatgtc tgcagttatt
    46441 ggacttgtgg caaatgttgt gcttaatata cttttaatac caattttagg tgctatggga
    46501 gctattatct ctacagttat tactgaattt attgtctctc tatatcaagc tatctcttta
    46561 agagatgtat ttacttttaa agaattaaca agaggaatgc tgcgctattt aattgctgct
    46621 actcttagtg gagctgtact ctattatatc aatactcaaa tgtcagtatc attggtaaat
    46681 tatgtcattc aaagtttagt agcagtaact atctatgtcg gtatcgtttt tataaccaaa
    46741 gcaccagtca ttcagttatt tagagatttt agaaaagagc atagaacatg aaatatttag
    46801 cgggtattgt cacctttaac cccaatattg aacgcttaga tcagaacatt cgagcaattt
    46861 atcctcaagt aagccatatc tacatcgtag ataatggctc gaaaaataag gaggaaattt
    46921 ctcaattagt ggccgattat aatgaagagg ggcacctaac tgttgattat ttaacagaaa
    46981 ataaaggaat tgcatatgct ttgaactgta ttggtcaatt tgcagtagca caagaatttg
    47041 attggttctt gactttagat caagattcgg ttgtcctagg tgatttgata gataattatg
    47101 aaaattacct tcatttacct aaggtcggta tgttatcttg tttgtatcaa gatatgaacc
    47161 gtgaaaattt agtgatgcaa gaatttgact ataaagaaat tgaagagtgt attacttcgg
    47221 ctgctttaat gaaaacgagc gttttcgaag aaacatctgg ctttgcagaa gagatgttta
    47281 ttgattttgt tgactctgaa atgaattatc gtctgtcaga gatgggatat aagacttatc
    47341 aggttaattt tattggtctg ttacacgaaa ttggacactc ttcacgagtt aaaaaatttg
    47401 gccatgtttt tcatgtattg aatcattcac catttcgaaa atattacatg atacgcaatg
    47461 cgatctatat tataaaaaaa tatggtaaga agaagcgata caaatattta gtatttatgc
    47521 gaaatgaatt tgttcgagtg ttggtagcag aagagcaaaa gagcaaaaaa atagttgcta
    47581 tgattaaagg cttaaaagat ggcttattga tgaaagtgga taaatatgga aaataaggta
    47641 aaaacagttg ctgttttctc aggatattat ctacccttcc tcggaggtat tgaacgttat
    47701 acagataaaa tgacagcaga tttggttaag cgtggctacc gtgttgttat tgtaacaact
    47761 aatcatggtg atttaccgat tatagatgaa gacaagggta gaaaaattta ccgtttaccg
    47821 accaaaaata ttgtcaagca gcgctatcct attattaaca aaaatcgaga atacaacacg
    47881 ctgatgaaat atgtttcaga tgaaaatatt gattttgtga tttgtaatac acgttttcag
    47941 ttaacaacgc tagaagggct atcttttgca aagaatcacc atttacctag tattgtttta
    48001 gaccatgggt caagtcattt ttctgtcaat aatcgttttt tagatttctt tggagctatc
    48061 tatgaacatc ttctcacagc gcgtgttaag cattatcgtc cagattttta tgcggtatcc
    48121 aagagaagcg ttgaatggtt aaaacatttt aatatagaag ctaaaggagt tatttataat
    48181 tcggtatctg aaagtctagg ctctgatttt gctggtacgg cttaccttga aaaatctgct
    48241 gatgatattt tcatcactta tgctggtaga attattaaag aaaaaggcat tgaattgctc
    48301 ttagaagcat tttcgatgtc acagtactct gaaaatgttt atttacagat tgcaggagac
    48361 ggacctgaat tagcacattt gaaagagaaa tatcagtcca aacagattaa ctttttgggg
    48421 aaattaaact ttgaacagac tatgtcttta atggcacaga ctgatatctt tgtttaccct
    48481 tcaatgtatc cagagggatt accaacctct attttagaag ctggtctact ttcaagtgct
    48541 attattgcaa cagatcgcgg tggaacagtt gaagttattg atagccctga gttaggaatc
    48601 atcatggaag aaaatactca gtcactccat gaatctcttg atttattagt aaaagataag
    48661 gcattaagag agaaactaca acagaatatt gctaaaagaa ttaaagaaca tttcacatgg
    48721 gaaaaaacag ttgaaaagtt ggattatatt attcaaaaaa attaacatta aaaagctaga
    48781 gca
//
LOCUS       DATRNK010000007        48546 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.007, whole genome shotgun sequence.
ACCESSION   DATRNK010000007 DATRNK010000000
VERSION     DATRNK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48546
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.007"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(97..2415)
                     /locus_tag="VBC52_000408"
     CDS             complement(97..2415)
                     /locus_tag="VBC52_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEO6882015.1"
                     /translation="MLDELKAIIKSPKLWITMAGVALIPTLYNVIFLSSMWDPYGNTK
                     NLPVAVVNQDKSAKLNGKTISIGKDMEDNLSKNDSLDFHFTTAKRAEKELEKGHYYMV
                     ITFPKDLSRKATTLMTEKPERLNITYKTTKGRSFVASKMSETAANKLKDEIAESITGT
                     YTESVFKNMGSMKTGINKAADGSQELLNGSNKLLDGSQTLTSNLDVLASSSQTFSGGA
                     NKLNSGINLYTDGVGTLSNGLETLSDGVTAYTTGVHKLSEGSQKLDDKSQALVEGSEK
                     LTDGLQQLSQATQLKPEQERTLQNLSDGLKNLNQIITNLQSTATTDSDTNSKLFNFLS
                     TIESSTKALMNTAAADKQKQMTAVQSTSAFKSLTPEQQSQITSAVTGTPTSAETIAAN
                     ISSNIENMKTVLSEASSSTPSNNNSQNLQTLSGTANNLVLKAISDLDKIQKLPTATKQ
                     LYQGSQKLTKGITDYTNAVGQLRKGAETLDSKSNQLISGTQKASQGAQTLDSKSDQLR
                     DGAGQLASGSDRIADGSNKLAGGGHQLTDGLTELSGGVSQLSSSLGKAGDQLSMVSVN
                     KDNANAVSSPVTIKHEDYDSVDTNGVGMAPYMISVALMVVALSANVIFAKALSGKEPD
                     NRFSWAKNKLLINGFIATLAATILFFAVQFIGLKPDYPGKTYFIILLTAWTLMALVTA
                     LVGWDNRYGSFLSLLILLFQLGSSAGTYPIELSPKFFQTIQPFLPMTYSVSGLRETIS
                     LTGDVNHQWRMLVIFLVSSMILALLIYRKQED"
     gene            2554..3093
                     /locus_tag="VBC52_000409"
     CDS             2554..3093
                     /locus_tag="VBC52_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEO6882016.1"
                     /translation="MVNDTRREKTKRAIEAAMITLLKDQSFDEISTINLTKTAGISRS
                     SFYTHYKDKYEMIDQYQQSLFNKVEYIFDRNQFKKEDALLEIFQFLDRESLFAALLTQ
                     NGTKEIQTYILNKLQLMLSKELPEVNPDATKSDINRLYYSVYLSHAIFGVYQMWITRG
                     KKESPQQITQVLLSLLPQT"
     gene            complement(3179..3475)
                     /locus_tag="VBC52_000410"
     CDS             complement(3179..3475)
                     /locus_tag="VBC52_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEO6882017.1"
                     /translation="MNLNDRLKIEEMEEKYDSFKPRINALVEAIDDFQKHYEDYVKLR
                     EFYGSEDWFRLSEQAENNLKCGVLSEDQLFDFIGEHNELVGQFLDMSSQMYRHL"
     gene            complement(3959..4306)
                     /locus_tag="VBC52_000411"
     CDS             complement(3959..4306)
                     /locus_tag="VBC52_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEO6882018.1"
                     /translation="MVSDNKTHSLIIPETVQEQLQEIKSYIETTYFSEQAGANTVNNI
                     LYGLERLEFFPEAGFNADDRVGETIYPPHNTRCIVLGDYLAFYHILEDRKAVFVSDII
                     HSKQDYIKLFKKK"
     gene            complement(4296..4583)
                     /locus_tag="VBC52_000412"
     CDS             complement(4296..4583)
                     /locus_tag="VBC52_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEO6882019.1"
                     /translation="MHALEKNTQVNFKTNSDLLEKAKAIIAAQNLDMTASFNLFLENI
                     VQNKALPFETDTDKERAELLAGLRAEIAKSFDDLEHGRVYNASEVRANLGI"
     gene            complement(4652..5029)
                     /locus_tag="VBC52_000413"
     CDS             complement(4652..5029)
                     /locus_tag="VBC52_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEO6882020.1"
                     /translation="MSKKNAIRKLKEFHRWQRIANSLDLSYNERYQFDIKYHPTRREH
                     LEISRECALEELDAIKYAINQLSKIDYRKILIECYLISEKKTQQDIIAELNKSQSWYY
                     ETKKRALLEFAQSYRNGVLITTA"
     gene            complement(5004..5156)
                     /locus_tag="VBC52_000414"
                     /pseudo
     CDS             complement(5004..5156)
                     /locus_tag="VBC52_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889234.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
     gene            complement(5163..5651)
                     /locus_tag="VBC52_000415"
     CDS             complement(5163..5651)
                     /locus_tag="VBC52_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882021.1"
                     /translation="MLELAIESIIKPMKKQEKTRVTGTINDQSITIDLDNLIVHYEGQ
                     EFLLETIPGTYGGKRYFFLCPDCERRCRKLFKASHAFACGTCQKLHQATFNRSKTDCQ
                     YYWQLAFKECLKVDSKAKHKHGYYSYDDFPKRPKYMRMTKYLYHWKRFHYYMDKGDKY
                     WL"
     gene            complement(5724..6281)
                     /locus_tag="VBC52_000416"
     CDS             complement(5724..6281)
                     /locus_tag="VBC52_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882022.1"
                     /translation="MRTFSDTPKTFTFHYTFKDFDTAQVACHAILGYMTGSYEQPVID
                     ATYHNDDQGGHANQLVLEYAEDRKLNKVFKRICDSFKDYYNQPEDMTDEELDDVAQET
                     ELLKEVESTDKKRVVPLFDNSQEKADKQDIFMAFISDHNQLAEHVSMNYKKMTQEDLG
                     AVLESISQGFNYLYDMAIEGELLVK"
     gene            complement(6439..6612)
                     /locus_tag="VBC52_000417"
     CDS             complement(6439..6612)
                     /locus_tag="VBC52_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2758 domain-containing protein"
                     /protein_id="HEO6882023.1"
                     /translation="MKIKLFYQKHKESLDDFEYRINQFTLSVSVIDIKFSEATYGNYE
                     DMSTTTSLLVLYR"
     gene            complement(6609..6866)
                     /locus_tag="VBC52_000418"
     CDS             complement(6609..6866)
                     /locus_tag="VBC52_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882024.1"
                     /translation="MNPNYTNHGIKKMHYHYDDLPNDQLYRLELPNGQCGYDFISKIN
                     FNTKSKPGDCDFTRPELIRRGYRWVFDCGFAIPVEKGEQTQ"
     gene            complement(7159..8553)
                     /locus_tag="VBC52_000419"
     CDS             complement(7159..8553)
                     /locus_tag="VBC52_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HEO6882025.1"
                     /translation="MKDYQNKLSQATQPAFAPAFRTRKGRGDKEYVISSPYNVGKVFE
                     FYENIFTGIKYNEFEKTIEITKTLPWSKEKGLWTNEQTSLCIAFIDDKYRFTPRKEHI
                     EVAITALAKKNSYHPIKQRIESQKWDGKARGERYFIDLLGCADNSYNREIAKVWLTGL
                     IARIYLRKVKFEVVPILIDKRQGTGKSTVTKRLLPSYHTDSEIKFGKNDSDYQKIQAN
                     AIIELGELKGMSKAEIETVKSFISSDSDTYREPYERKATPHPRHCVFIGTANKKSFLK
                     DSGTERRFFPIECGINDVEKHPMEVEEDYFLQILAEAKVWFNNYEPLTPSKELMNQLA
                     DIQEDYKVEDVDKEIIDQLLNEFQIVEGWDSLSQYEQRQYVLKQLGEPLDNAQSYSDY
                     PSAQTNCLLQVTSPNHIAYLGFNQKPTQGGKALISQKIRDHLDNDDGWKKGENPARKR
                     LFKGGTPVSYYERV"
     gene            complement(8565..9422)
                     /locus_tag="VBC52_000420"
     CDS             complement(8565..9422)
                     /locus_tag="VBC52_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEO6882026.1"
                     /translation="MAIYEARGFSSYLYPYKGQLEPFDYIAQFKPMKTPEAIDIEEYK
                     RTQAPYCLSGKVTPEKNGSYKRNNASLVYRDLIFLDYDEIETDVNLPKIVSKTLWEYS
                     YIIYPTIKHTPEKPRYRLVVKPSDTMDKTTYKQVVKEIANKIGLPFDLASLTWSQLQG
                     LPVTTGDPEDYQRYVNRGLDYPVPKAPNRQVTTTYTSRPRSQRSITMRVIDTLFNGFG
                     DEGGRNVALTKFVGLLFNKWVDCDLETAYELVQIANSVTTKPLPIDEIDKTFRSILDT
                     ELRKRGIKP"
     gene            complement(9423..9695)
                     /locus_tag="VBC52_000421"
     CDS             complement(9423..9695)
                     /locus_tag="VBC52_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882027.1"
                     /translation="MNVFSQDYEAKLLEQNLTAFNRFLEVYQQPKPRVLGLITAEQVK
                     EELNIKGKTLKRWENAGLKRYQPPLEDTRKHYYKVSDILIFLGVDV"
     gene            complement(10037..10228)
                     /locus_tag="VBC52_000422"
     CDS             complement(10037..10228)
                     /locus_tag="VBC52_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882028.1"
                     /translation="MQELNFTLTQTLILFIVLGLIGLLLSRSKPLIEIDLPEDTQAPK
                     PRQNANYGAYIQAQNHYYN"
     gene            complement(10231..10452)
                     /locus_tag="VBC52_000423"
     CDS             complement(10231..10452)
                     /locus_tag="VBC52_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882029.1"
                     /translation="MRSIEELNEIANDILVDYEGLCGQLMDILNVLDLAAQEYSKETT
                     SAIINTSCRALKCLISDHDKMTHKYRKEL"
     gene            complement(10449..10781)
                     /locus_tag="VBC52_000424"
     CDS             complement(10449..10781)
                     /locus_tag="VBC52_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882030.1"
                     /translation="MKYRVETNPFSKDRYTPEQREMLKKRQLSKDKAEAYFARLYNHH
                     IARVIIANVMAEYTTTFRKSATTFEEAWGALGYKQTTEIVFRAVNGLPCSEKDTGELE
                     TYLSEVSA"
     gene            complement(10858..11364)
                     /locus_tag="VBC52_000425"
     CDS             complement(10858..11364)
                     /locus_tag="VBC52_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882031.1"
                     /translation="MRTLFQSTQKTERECAFCVLKNQNNMIKKKRNPRVLFKNVAYKL
                     SEIEGKTLTEIASFLGFGNSEVCRSTLYNWKRRKWLTFDLKNGHYRNVEVLHEVTLEK
                     MANKELKEQGLIYKASIYYEQVVSTSEIIEDIKTKTQDRIKAIHLQQKALERIPSELF
                     AELYTNMN"
     gene            complement(11453..11626)
                     /locus_tag="VBC52_000426"
     CDS             complement(11453..11626)
                     /locus_tag="VBC52_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEO6882032.1"
                     /translation="MTDKRAVGRPPKGEQPNNIKITVRISQGLDEKVQAYADNHGLTK
                     PEAIRKALEDLSD"
     gene            complement(11725..12363)
                     /locus_tag="VBC52_000427"
     CDS             complement(11725..12363)
                     /locus_tag="VBC52_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HEO6882033.1"
                     /translation="MNIIKQVKSSFGELEIDFYLDRNRNIFVTIEQLAQRFGYKSRNA
                     IEKMIERQPYLKEKRFSVTDKLSATDGKQYETRLFNKRGIFEIGMLSKTEKGKIFRQW
                     IYDHIEELERENANFKLIRELEKSNHKELTQAIKDWEHFNQWSYKAISDLLLKSVTGQ
                     TAKQLKQSRVGYEIALDCLSADELTRYRKLEQKVIVLLELNAEYNDIKKLVL"
     gene            complement(12599..13201)
                     /locus_tag="VBC52_000428"
     CDS             complement(12599..13201)
                     /locus_tag="VBC52_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HEO6882034.1"
                     /translation="MRTETWNGYDIRFVEHQGEWWAVLADICHALDLKPKRVKERLVD
                     EVVSTDHVADSLGRQQEMLIVNEFGIYDTIFSSRKPEAKSFKFWVFETIKQLRQATGL
                     EGFEVFRMLDKEHQKEAMARLTNSLDRVSKKDLIKANTITNKAVSNKFGYSKMVKKSE
                     MTPDMLVAREMILDDTVELMGIKEKFGLNISVSESIYNKN"
     gene            complement(13217..13402)
                     /locus_tag="VBC52_000429"
     CDS             complement(13217..13402)
                     /locus_tag="VBC52_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882035.1"
                     /translation="MLITSTQAKAIRRKQADKQLTSKSASAEIGVTEVTYRKVRNGGE
                     VKNGIYQKVMEWLAEDY"
     gene            13557..14144
                     /locus_tag="VBC52_000430"
     CDS             13557..14144
                     /locus_tag="VBC52_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6882036.1"
                     /translation="MNRLKELRANQKKTQQDMSEFLNMSRRGYQKIENGESQIKPDKA
                     QALADYFNVSVGYLLGYSDDKKDPMLTSFELARKDDTGEYFDLQDMVTLMDISMLKNV
                     DTRDKILSNLKEYYDYYGIRMKEEHEIKDSEKFNAFLAEFQEDTNDYIIMLLTGIVKL
                     RDDIRFTLLDILAMEGEKLETVKNVVKFISDNGDN"
     gene            14399..15556
                     /locus_tag="VBC52_000431"
     CDS             14399..15556
                     /locus_tag="VBC52_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbhA"
                     /protein_id="HEO6882037.1"
                     /translation="MKKRIIVVLVALLAIGGGSAYYFLSYAPHQEAVTNFNKAVQTVK
                     SKNKTLKSEIAKAEKLVKSNQQPLDVRTLDNLKAAVATAKSDLREIPKVADKTEAIKK
                     QTKSLNQPIDYSKATQSLADSSLAYTNSVKQLAQITNPSQSFVEERLKEIDTVTGVQS
                     VTESNDPNGKLNKQGGYTASIYFTDNQVTEAVEGKDVLDKGTDAGGNIEVFKTKEEAE
                     KRNIYLSAFDSAGMFNPGSHYVFGTVVIRTSHYLTASQQKSLTQKIYEKLIEIKSKNS
                     GSTKDSKKKEVSKDNTKTSTQEQPAPSSTTDKSQESQSSSETSNSGGRGGTRGVEDSS
                     DTSSSSEMTEEELNSAIKEIQDSRDYYAPNEFENQVGDTTSSDNGDSTYHP"
     gene            16573..17757
                     /locus_tag="VBC52_000432"
     CDS             16573..17757
                     /locus_tag="VBC52_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6882038.1"
                     /translation="MERFIIVKINEVKKKDGTVIYRASVYLGTDKVTGKKVTTKITGR
                     TKKEVREKAKQGAVEFIKNGSTRFKATSVTSYQELATLWWDSYKHTVKYNTQLATEKL
                     LTVHVIPIFGAYKLDKLTTPLIQSIINKLADKTNKGERKAYLHYDRIHALNKRILQYG
                     VIMQAIPFNPAREVILPRNTKKANTKRVKHFENDELRTFFNYLNNLDKSKYRNFYEVT
                     LYKFLLATGCRINEALALNWSDIDLDNAVVHITKTLNYKQEINSPKSKSSYRDIDIDS
                     QTITMLKQYRRRQIQEAWKLGRSETVVFSDFIHKYPNNRTLQTRLRTHFKRANVPNIG
                     FHGFRHTHASLLLNTGIPYKELQYRLGHSTLSMTMDIYSHLSKENAKKAVSFFETAIN
                     SI"
     gene            complement(17865..18476)
                     /gene="rpsD"
                     /locus_tag="VBC52_000433"
     CDS             complement(17865..18476)
                     /gene="rpsD"
                     /locus_tag="VBC52_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEO6882039.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKAKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(18806..19093)
                     /locus_tag="VBC52_000434"
     CDS             complement(18806..19093)
                     /locus_tag="VBC52_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEO6882040.1"
                     /translation="MSDAFADVAKMKKIKEDIKSHEGQMVELTLENGRKREKNKIGRL
                     IEVYPSLFIVEYKDTAAVPGAIDNTYVESYTYSDILTEKTLIRYFDDESAE"
     gene            complement(19105..20460)
                     /gene="dnaB"
                     /locus_tag="VBC52_000435"
     CDS             complement(19105..20460)
                     /gene="dnaB"
                     /locus_tag="VBC52_000435"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEO6882041.1"
                     /translation="MAEVSELRVQPQDLLAEQAVLGSIFISPEKLIMVREFISPDDFY
                     KYSHKVIFRAMITLADRNDAIDAATVRNILDDQGDLQNIGGLGYIVELVNSVPTSANA
                     EFYAKIVSEKAMLRDIISKLTDTVNMAYEGNDSDEIIATAEKALVDINEHSNRSGFRK
                     ISDVLKVNYENLELRSQQTSDVTGLPTGFRDLDRITTGLHPDQLIILAARPAVGKTAF
                     VLNIAQNVGTKQNRPVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNVT
                     IAQGALADAPIYIDDTPGIKITEIRARSRKLSQEVDDGLGLIVIDYLQLISGTRPENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYRREGEEAEEIVEDNTVEVILEKNRAGARGTVKLMFQKEYNKFSSIAQFE
                     E"
     gene            complement(20503..20955)
                     /gene="rplI"
                     /locus_tag="VBC52_000436"
     CDS             complement(20503..20955)
                     /gene="rplI"
                     /locus_tag="VBC52_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEO6882042.1"
                     /translation="MKVIFLQDVKGKGKKGEVKEVPTGYAQNFLLKKNLAKEATTQAI
                     GELKGKQKSEEKAQAEILAQAQELKTQLESETTRVQFIEKVGPDGRTFGSITAKKIAE
                     ELQKQYGIKIDKRHIDLDHTIRAIGKVEVPVKLHKQVSSQIKLDIKEA"
     gene            complement(20961..22943)
                     /locus_tag="VBC52_000437"
     CDS             complement(20961..22943)
                     /locus_tag="VBC52_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEO6882043.1"
                     /translation="MKRFRFATVHLVLIGLILFGLLAICVRLFQSYTALLLAIFVALS
                     FVVALLYYQKITYELSEVEQIELLNDQTEVSLKSLLEQMPVGVIQFDLETNDIEWFNP
                     YAELIFTGDNGHFQSATVKDIITSRRNGTAGQSFEYGDNKYSAYLDTETGVFYFFDNF
                     MGNRRNYDSSMLRPVIGIISIDNYDDIMDTMLEADMSKINAFVTSFISDFTQSKNIFY
                     RRVNMDRYYIFTDYSVLNTLIKDKFDILNEFRKRAQENHLSLTLSMGISYGDGNHNQI
                     GQIALENLNTALVRGGDQIVVRENDSSKKALYFGGGAVSTIKRSRTRTRAMMTAISDR
                     LKVVDSVFIVGHRKLDMDALGASVGMQFFASNIVNASYVVYDPNDMNSDIERAIDYLQ
                     EDGETRLVSVERAFELITQNSLLVMVDHSKTALTLSKEFFNKFADVIVVDHHRRDEDF
                     PKNAVLSFIESGASSASELVTELIQFQQAKDKLSRSQASILMAGIMLDTRNFASNVTS
                     RTFDVASYLRGLGSNSMAIQKISATDFDEYRLINELILKGERIYDNIIVATGEEHKVY
                     SHVIASKAADTMLTMAGIEATFVITKNSSNIGISARSRNNINVQRIMEKLGGGGHFSF
                     AACQIQDKSVKQVRRMLLEIIDEDLRENSTVENRRD"
     gene            complement(23010..24911)
                     /gene="mnmG"
                     /locus_tag="VBC52_000438"
     CDS             complement(23010..24911)
                     /gene="mnmG"
                     /locus_tag="VBC52_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEO6882044.1"
                     /translation="MTHNFAENYDIIVVGAGHAGVEASLAASRMGCKTLLATINLEML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KALYAQTMKQTVEKQENLTLRQAMIDEILVEDGKVVGVRTATNQKFSAKSVVITTGTA
                     LRGEIILGELKYSSGPNNSLASVTLADNLRDLGLEIGRFKTGTPPRVKASSINYEETE
                     IQPGDEQPNHFSFMSRDEDYITDQVPCWLTYTNTLSHDIINQNLHRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRHTEEVYVQGLSTSLPEDVQVDLLRSIKGL
                     ENAEMMRTGYAIEYDIVLPHQLRATLETKVIAGLFTAGQTNGTSGYEEAAGQGLVAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGYEIGLVDEERYAIFKKRQMQFENELERLDSIKLKPVSETNKRIQELGFKPLTD
                     ALTAKEFMRRPQITYAVATDFVGCTDEPLDSKVIELLETEIKYEGYIKKALDQVAKMK
                     RMEEKRIPPHIDWDDIDSIATEARQKFKKINPETLGQASRISGVNPADISILMVYLEG
                     RQKGRKNIN"
     gene            complement(25081..25692)
                     /locus_tag="VBC52_000439"
     CDS             complement(25081..25692)
                     /locus_tag="VBC52_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEO6882045.1"
                     /translation="MISKFILAFMAFFAIMNPISNLPAFMALVADDDQKISRRIAAKG
                     VLLAFVIIVIFVLSGHLLFNLFGITLAALKISGGILVGIIGYKMINGIHSPTNKNLEE
                     HKDDPMNVAVSPLAMPLLAGPGTIATAMGLSSGGLSGKLITILAFAILCVIMYVILIS
                     ANEITKFLGKNAMTIITKMMGLILMTIGIEMLITGIKIGFHLT"
     gene            complement(25724..26845)
                     /gene="mnmA"
                     /locus_tag="VBC52_000440"
     CDS             complement(25724..26845)
                     /gene="mnmA"
                     /locus_tag="VBC52_000440"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEO6882046.1"
                     /translation="MTDNSNIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVTRDKNGIVHMLRGVDNNKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKPEVRRIAEEAGLATAKKKDSTGICFIGEKNFKDFLGQYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNKPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLTASEIHFTRDMPNEFKLECTAKFRYRQPDSKVTVYVKGNQARVVFD
                     DLQRAITPGQAVVFYNEQECLGGGMIDQAYRDDKICQYI"
     gene            27091..27759
                     /gene="sdaAB"
                     /locus_tag="VBC52_000441"
     CDS             27091..27759
                     /gene="sdaAB"
                     /locus_tag="VBC52_000441"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEO6882047.1"
                     /translation="MKHLKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGEPSE
                     VTFHLYNSFAKTYQGHGTDKALVAGILGMDTDNPDIKNSLEIAHQKGIKIYWDILKDS
                     NSPHPNTAKITVKNGDRSMSITGVSIGGGNIQVTELNGFSVSLTMNTPTLIIVHQDIP
                     GMIAKVTDILSDFNINIAQMNVTRESAGEKAIMIIEVDSRDCQQAVKKIEAIPHLHNV
                     NFFD"
     gene            27774..28646
                     /gene="sdaAA"
                     /locus_tag="VBC52_000442"
     CDS             27774..28646
                     /gene="sdaAA"
                     /locus_tag="VBC52_000442"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEO6882048.1"
                     /translation="MFYTIEELVEQANSQHKGNIAELMIQTEIEMTGRSREEIRYIMS
                     RNLEVMKASVIDGLTPSKSISGLTGGDAVKMDQYLQSGKTISDTTILAAVRNAMAVNE
                     LNAKMGLVCATPTAGSAGCLPAVISTAIEKLNLTEEEQLNFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSASAMAAAALVMAAGGTPFQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNALGSSFALVAADMALAGIESQIPVDEVIDAMYQVGSSLPTAFRETAEGGLAA
                     TPTGRRYSKEIFGE"
     gene            complement(28780..29454)
                     /locus_tag="VBC52_000443"
     CDS             complement(28780..29454)
                     /locus_tag="VBC52_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEO6882049.1"
                     /translation="MNKRRKLSKMNVKKQHLAYGAITLVALFSCILAVTVIFKSSQVT
                     TESLSKADKVRVAKKSKMNKATSKSKVEGVKQAPKPSSQSTEANSQQQVTASEEAAVE
                     QAVVTENTPATSQAQQAYAVTETTYRPAQHQPSGQVLSNGNTAGAIGSAAAAQMAAAT
                     GVPQSTWEHIIARESNGNPNVANASGASGLFQTMPGWGSTATVQDQVNSAIKAYRAQG
                     LSAWGY"
     gene            complement(29560..30099)
                     /locus_tag="VBC52_000444"
     CDS             complement(29560..30099)
                     /locus_tag="VBC52_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO6882050.1"
                     /translation="MSITSVKKSKPFKLGVAGLLVGASLALPLSVSAASYTVKSGDTL
                     SAIAKNHKTTVQELVSLNSISNADVISIGDVLKLDNSTTSQAEAKSQPTIENSMNSSS
                     NLSSSDSAAKEEIARRESNGSYTAQNGQYYGRYQLSQSYLNGDLSPENQEKVADNYVA
                     SRYGSWSAALSFWNSNGWY"
     gene            complement(30315..31109)
                     /locus_tag="VBC52_000445"
     CDS             complement(30315..31109)
                     /locus_tag="VBC52_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO6882051.1"
                     /translation="MDKLILGRYIPGNSLIHKLDPRSKLLAMLLFIIIVFWANNVVTN
                     VIVFIFTLVIVGLSQIKFSYFFNGIKPMVGIILFTTLFQMLFAQGGQVIFSFWIFSIT
                     SLGLQQAALIFMRFVLIIFFSTLLTLTTTPLSLADAVESLLKPLEVLRVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIHKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGANRSKYRLLKWTVRDTFSILLMLLLGLSLFLLKN"
     gene            complement(31102..31944)
                     /locus_tag="VBC52_000446"
     CDS             complement(31102..31944)
                     /locus_tag="VBC52_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO6882052.1"
                     /translation="MGIEFKNVSYTYQAGTPFEGRALFDVNLKIEDASYTAFIGHTGS
                     GKSTIMQLLNGLHIPTKGEVIVDDFSIKAGDKNKEIKFIRQKVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGISQIEAERLAEEKLRLVGISEDLFDKNPFELSGGQMRRVAIAGIL
                     AMEPKVLVLDEPTAGLDPKGRKELMTLFKNLHKKGMTIVLVTHLMDDVADYADYVYVL
                     EAGKVTLSGQPKQIFQEVELLESKQLGVPKITKFAQRLSHKGLNLPSLPITINEFVEA
                     IKHG"
     gene            complement(31920..32759)
                     /locus_tag="VBC52_000447"
     CDS             complement(31920..32759)
                     /locus_tag="VBC52_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO6882053.1"
                     /translation="MTNIITVNNLFFKYDSNQTHYQLENVSFHVKQGEWLSIIGHNGS
                     GKSTTVRLIDGLLEAESGQIIIDGQELTEDNVWELRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIPLKDMKERVGQALDLVGMSEFKMREPARLSGGQKQRVAIAGAVAMRPQ
                     VIILDEATSMLDPEGRLELIRTIRAIRQKYNLTVISITHDLDEVALSDRVIVMKNGKV
                     ESTSTPKALFGRGNRLISLGLDVPFTSRLMAALAANGLDIGTEYLTEKELEEQLWELN
                     LKM"
     gene            complement(32759..33301)
                     /gene="pgsA"
                     /locus_tag="VBC52_000448"
     CDS             complement(32759..33301)
                     /gene="pgsA"
                     /locus_tag="VBC52_000448"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEO6882054.1"
                     /translation="MVKKENIPNLLTVVRILMIPLFIVLTSVTTSTTWHIVAAIVFAI
                     ASLTDYLDGYLARKWQVVTNFGKFADPLADKMLVMSAFIMLVGLGLAPAWVSAIIICR
                     ELAVTGLRLLLVETGGTVLAAAMPGKIKTATQMFAVIFLLVHWMTLGNIMLYIALFFT
                     LYSGYDYFKGAGFLFKDTFK"
     gene            complement(33424..34707)
                     /locus_tag="VBC52_000449"
     CDS             complement(33424..34707)
                     /locus_tag="VBC52_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO6882055.1"
                     /translation="MTVNKITYQNLQEEVYKLTLESGLNVYLIPKPSFKETVGVLTAN
                     FGSLHTRYTRNGCVEHYPAGIAHFLEHKLFELDKGQDAATQFTKYGAESNAFTTFDKT
                     SFYFSTISHITNCLDILLDFVLTTNFTEESITKEKDIIKQEIEMYQDDPEYRLYQGVL
                     SNLYPNSPLAFDIAGDYQSISQITLTDLQENHKDFYQLSNMNLVLVGQFSPQEIITYL
                     QKNSHLTNYSQNIDRDSISLEPVIKNNSCHMTVTKPKLAIGYRKSSHMRHGSYLKEKI
                     GLQLFFAMLLGWTSTINQDWYESGQIDDSFDIEIEVHPDFECVIISLDTTEPIAFSTQ
                     LRLLLKNALQSSDLTESHLQNVKRELYGDFLRSLDSIENLAMQFVTYLYDGKTMYLDL
                     PSIVEELDLEDVITIGKDFLDNADTSDFVIFPKSS"
     gene            complement(34709..35953)
                     /locus_tag="VBC52_000450"
     CDS             complement(34709..35953)
                     /locus_tag="VBC52_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO6882056.1"
                     /translation="MKIVEGVSLHLIKNQQFKTNHLTFRFSGDFNNKTVARRSLVAQM
                     LVTANAKYPKVQEFREKLASLYGASLSTKISTKGLVHIVDIDIVFVKNTFTLEQENIV
                     EQIITFLEDMLFSPLISLEQYQTSIFDTEKKNLIQYLEADIEDNFYSSDLALKSLFYN
                     NKTLRLPKYGTASLVESENSFTAYQEFQKMLKEDQLDIFVVGDFDDYRMIQAFNRMAF
                     EPRHKVLAFDYTQTYENITRSQVEDKDVNQSIMQLAYHLPITYKDEDYFALIVFNGLF
                     GAFAHSLLFTEIREKQGLAYTIGSQFDSFTGLFTIYAGIDKENRERFLKLINKQFNNI
                     KMGRFSSTLLKQTKDILKMNYVLASDNPKVIVDHIYHEHYLDQFHTSALFIDKVDDVT
                     KSDIVSVATKLKLQAFYFLEGN"
     gene            36192..36545
                     /gene="yaaA"
                     /locus_tag="VBC52_000451"
     CDS             36192..36545
                     /gene="yaaA"
                     /locus_tag="VBC52_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEO6882057.1"
                     /translation="MDYKLFDEYITLQSLLKEIGIIQSGGAIKKFLADNQVLFNGDLE
                     NRRGKKLRLGDIITIPDQNVEIIIRKPSDQEIEERNVEIAEKQRVSAIVKEMNKNTNK
                     DKSKTSKKPVRFPGT"
     gene            36548..37657
                     /gene="recF"
                     /locus_tag="VBC52_000452"
     CDS             36548..37657
                     /gene="recF"
                     /locus_tag="VBC52_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEO6882058.1"
                     /translation="MWIKNISLKHYRNYEEAQVDFSPNLNIFIGRNAQGKTNFLEAIY
                     FLALTRSHRTRSDKELVHFKHHDVQITGEVIRKSGHLSLDIQLSEKGRITKVNHLKQA
                     KLSDYIGAMTVVLFAPEDLQLVKGAPSLRRKFLDIDIGQIKPTYLAELSNYNHVLKQR
                     NTYLKTTNNVDKTFLSVLDEQLADYGSRVIEHRFDFIQALNDEADKHHYIISTELEHL
                     SIHYKSSIEFTDKSSIREHFLNQLSKSHSRDIFKKNTSIGPHRDDITFFINDINATFG
                     SQGQQRSLILSLKLAEIELIKTVTNDYPILLLDDVMSELDNHRQLKLLEGIKENVQTF
                     ITTTSLEHLSALPDQLKIFNVSDGTISINEKKATD"
     gene            complement(37669..38535)
                     /locus_tag="VBC52_000453"
     CDS             complement(37669..38535)
                     /locus_tag="VBC52_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEO6882059.1"
                     /translation="MEGLLIALIPMFAWGSIGFVSNKIGGRPNQQTFGMTLGALLFAI
                     IVWLFKQPEMTASLWIFGILGGILWSVGQNGQFQAMKYMGVSVANPLSSGAQLVGGSL
                     VGALVFHEWTKPIQFILGLTALTLLVIGFYFSSKRDVSEQALATHQEFSKGFATIAYS
                     TVGYISYAVLFNNIMKFDAMAVILPMAVGMCLGAICFMKFRVNFEAVVVKNMITGLMW
                     GVGNVFMLLAAAKAGLAIAFSFSQLGVIISIIGGILFLGETKTKKEQKWVVMGILCFV
                     MGAILLGIVKSY"
     gene            complement(38591..39253)
                     /locus_tag="VBC52_000454"
     CDS             complement(38591..39253)
                     /locus_tag="VBC52_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6882060.1"
                     /translation="MKNYGKILKELREDKGISLSSLAKSAQLSKSTLSRFENGETQIG
                     IDKFIKALQTLEVRVTINEVSILDSKVKAGTSNTDLEQLTLLESYRDNEDIMRIFSFQ
                     KQQSCDRIESNVLKILAKLFISNLGLNMRLPQDEINLVVTYLNGVTQYNDFYFKVICY
                     FQDILPKDVILNKISNMTKEQLPYSKSLVNLLIKQVIIALEKDSVDKAIVFADFANKV
                     SQ"
     gene            complement(39328..40809)
                     /gene="guaB"
                     /locus_tag="VBC52_000455"
     CDS             complement(39328..40809)
                     /gene="guaB"
                     /locus_tag="VBC52_000455"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEO6882061.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNEVDMKTKLADNLTL
                     NIPIITAAMDTVTDSKMAIAIARAGGLGIIHKNMSIVDQAEEVRKVKRSENGVIIDPF
                     FLTPDNTVSEAEELMQNYRISGVPIVETLENRKLVGIITNRDMRFISDYKQLISEHMT
                     SQNLVTAPIGTDLETAERILHEHRIEKLPLVDDEGRLSGLITIKDIEKVIEFPKAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQITAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIVFQMLGGIRSGMGY
                     VGAANIKELHDNAQFVEMSGAGLKESHPHDVQITNEAPNYSVH"
     gene            complement(40966..41451)
                     /locus_tag="VBC52_000456"
     CDS             complement(40966..41451)
                     /locus_tag="VBC52_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO6882062.1"
                     /translation="MNKKETRHQLIRSLVSETKVRTQHELRELLEKNGVSVTQATLSR
                     DMKELNLIKVNESSDNATETYYEIHSISQKRWEERLRFYMEDALVMLFPVQNQIVLKT
                     LPGLAQSFGSILDSILLPEILATVCGDDTCLIICNNSEEAQKCFEKLSQYTPPFFFSK
                     K"
     gene            complement(41461..42141)
                     /locus_tag="VBC52_000457"
     CDS             complement(41461..42141)
                     /locus_tag="VBC52_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO6882063.1"
                     /translation="MITKEQYFYFRQLDDFKHFTIEQFDHIVSHIKHRTALKNHTLFF
                     EGDYREKLFLIQSGHVKIEQSDASGSFIYTDYVRQDTVFPYGGLFLDDDYHFSAVAIT
                     DIEYFSLPMALYEEYSLQNINQMKHLCRKYSKLLRVHEIRLRNMVTSSASMRVIQSLA
                     TLLLQVPTERGHLPFPITTIEIANMSGTTRETVSHVLKELRQKDIVEMKGKKLLYNNK
                     NYFKKFIE"
     gene            42349..43053
                     /locus_tag="VBC52_000458"
     CDS             42349..43053
                     /locus_tag="VBC52_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEO6882064.1"
                     /translation="MTKFIVDSSYWNLFPTSKIGVILIKDYHMDRNLETELKQLLSDS
                     HSLAKKYLQEKEFSQNRVIQTYRKAYQTFKTKKGARSSIEALLKRVNSGNEITSINPL
                     VDIYNAASLRFGLPIGAEDSDTFRGDLKLTITNGGDEFYLIGEDFNRPTLSGELAYVD
                     DVGAVCRCFNWRDGKRTMITDNTQNAFLVIELIDNGREIIFKEALDFIATNTNRFLKA
                     KTQTIILDKEHSEITL"
     gene            43320..44552
                     /gene="arcA"
                     /locus_tag="VBC52_000459"
     CDS             43320..44552
                     /gene="arcA"
                     /locus_tag="VBC52_000459"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEO6882065.1"
                     /translation="MTQTHPIHVFSEIGKLKKVMLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRNEGVEVLYLENLAAESLTNQGIREQFIDEYIGEANVRGRA
                     TKKAIRELLLNIKDNKELIEKTMAGIQKSELPEIPSSEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGNGVSLNHMFSETRNRETLYGKYIFTHHPEYGGKVPMVYEREETTRI
                     EGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHLD
                     TVFTMVDYDKFTIHPEIEGDLRVYSVTYENQDLHIEEEKGDLADLLAKNLGVEKVELI
                     RCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKINGSELV
                     RGRGGPRCMSMPFEREDL"
     gene            44558..44989
                     /locus_tag="VBC52_000460"
     CDS             44558..44989
                     /locus_tag="VBC52_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6882066.1"
                     /translation="MKLLVNHLPYQRAASLYIRFSVFVIERNIKMEEEFDDNDEQDTI
                     YAVLYDGKQPVSTGRFLPETQTEARLTRIATLKGYRGNGYGTKIIIALENYAKENGYH
                     YLTIHAELTAKDFYQTLGYQATGDIYMEDGEACQTLEKYLI"
     gene            45005..46018
                     /gene="argF"
                     /locus_tag="VBC52_000461"
     CDS             45005..46018
                     /gene="argF"
                     /locus_tag="VBC52_000461"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO6882067.1"
                     /translation="MTQVFQGRSFLAEKDFSREEFEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTIKENFGKLEGITLVY
                     CGDGRNNVANSLLVAGTLMGVNVHIFSPKELFPAEEIVKLAEGYAKESGAHVLVTDNV
                     DEAVKGADVFYTDVWVSMGEEDKFKERVELLQPYQVNMDLIKKANNDNLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            46081..47508
                     /gene="arcD"
                     /locus_tag="VBC52_000462"
     CDS             46081..47508
                     /gene="arcD"
                     /locus_tag="VBC52_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEO6882068.1"
                     /translation="MEKEKKLGLLPLTMLVIGSLIGGGIFDLMQNMSSRAGLVPMLIA
                     WVITAIGMGTFVLSFQNLSEKRPDLTAGIFSYAKEGFGNFMGFNSAWGYWLSAWLGNV
                     AYAALLFSSLGYFFKFFGNGNNIISIIGASIVIWVVHFLILRGVNTAAFINTIVTFAK
                     LVPVIIFLISALLAFKFNIFSLDIWGNGLHQSIFNQVNSTMKTAVWVFIGIEGAVVFS
                     GRAKKHSDIGKASILALFTMISLYVLISVLSLGIMSRPELANLKTPAMAYVLEKAVGH
                     WGAILVNLGVIISVFGAILAWTLFAAELPYQAAKEGAFPKFFAKENKNKAPINSLLVT
                     NLCVQAFLITFLFTQSAYRFGFALASSAILIPYAFTALYQLQFTLREDKSTPGHQKNL
                     IIGILATIYAVYLIYAGGFDYLLLTMIAYTLGMILYIKMRKDDKLPIFVGYEKISAIV
                     ILALCLLCIIEIMTGQIDIAAILAG"
     gene            47529..48485
                     /gene="arcC"
                     /locus_tag="VBC52_000463"
     CDS             47529..48485
                     /gene="arcC"
                     /locus_tag="VBC52_000463"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO6882069.1"
                     /translation="MVKEHQKIVVALGGNAILSTDASAKAQQEALINTSKSLVKLIKE
                     GHDVIVTHGNGPQVGNLLLQQAAADSEKNPAMPLDTCVAMTEGSIGFWLQNALNNELQ
                     EQGIDKEVATVVTQVIVDEKDQAFTNPTKPIGPFLSEEDAKKQAQETGSKFKEDAGRG
                     WRKVVPSPKPVGIKEASVIRRLVDSGVVVISAGGGGVPVIEDANTKALKGVEAVIDKD
                     FASQTLSELVDADLFIVLTGVDNVFVNFNKPNQEKLEEVTVSQMKQYITENQFAPGSM
                     LPKVEAAIAFVENKPEARAIITSLENIDNVLAENAGTQIVAN"
BASE COUNT        15378 a         9099 c         8041 g        16028 t
ORIGIN      
        1 tgttgcaata attattgtta aagtagacat atcttctcct cctaaatact ttgacatacc
       61 atatcatact gttagtctat cactagatac tttctattaa tcttcttgtt tacgataaat
      121 aagaagagca agtatcatcg atgatactaa aaagattact agcattctcc attgatggtt
      181 aacgtctccc gtcaacgaga tggtctctct taatcctgaa acagagtaag tcatcggtaa
      241 aaatggttga attgtttgaa agaacttagg actcaattct attgggtaag ttcctgcgct
      301 tgaaccaagc tggaataata atattaataa cgacaagaag gaaccatatc tattatccca
      361 tcccactaaa gcagttacta atgccatcaa agtccatgct gtcaatagga taataaagta
      421 tgtttttcca gggtaatctg gtttaagacc tatgaattga actgcaaaaa ataagatagt
      481 tgctgctaga gttgcaatga atccattgat taataattta tttttagccc aactaaaacg
      541 attatctggc tctttgccag ataaagcttt cgcaaaaata acattggctg ataaagctac
      601 caccatcaac gcaactgaaa tcatgtatgg tgccattcca acaccattcg tatcaacgct
      661 atcataatct tcatgcttga tagtgactgg gctagatact gcattggcat tatctttatt
      721 tacagaaacc atggacaact ggtccccagc tttccccaaa gaacttgata attgagaaac
      781 accacctgac aactctgtca acccatctgt caactgatgt cctcccccag caagcttatt
      841 agaaccatct gcaattctat cagaaccact agctaattgc cccgctccgt ctctcaattg
      901 atccgattta ctatctaaag tttgagctcc ctgacttgct ttttgtgttc ctgaaatcaa
      961 ttgattggat ttactatcta aagtttctgc acctttgcgt aattgcccta cagcattcgt
     1021 ataatccgtg attccttttg tcaatttttg actaccttga tataactgct tagtagccgt
     1081 tggcaatttt tgaattttat ctaaatctga gatagctttt aatactaaat tgttagctgt
     1141 tcctgacaaa gtttgaagat tttgtgagtt attattagaa ggggttgaac tagatgcttc
     1201 actaagcaca gttttcatat tttctatatt tgaagaaata tttgctgcaa ttgtctcagc
     1261 tgaagtaggt gttccagtaa ccgctgaggt aatttgagat tgttgctcag gggttaaact
     1321 tttaaaagct gacgttgatt gtaccgcagt catttgcttt tgtttatcag cagcagccgt
     1381 attcatcaaa gcctttgtac ttgattcaat agttgataaa aaattaaata acttactatt
     1441 agtatcgcta tcagtagttg ctgtcgattg taaatttgta ataatctgat ttagattctt
     1501 taatccatct gaaaggttct gcaaagtacg ttcttgctct ggttttagtt gagttgcttg
     1561 tgacaattgt tgtaaaccat ccgtcaactt ttctgatcct tccaccaatg cttgagactt
     1621 atcatctaat ttttgactac cttcactcaa cttatgcaca cctgttgtgt aagcagtaac
     1681 accatctgat aatgtttcta acccgttaga tagggtcccc acaccatctg tataaaggtt
     1741 tattccacta tttagtttat tagctcctcc agaaaaagtt tgactagagc ttgctaaaac
     1801 atctaaatta cttgtcaagg tttgactgcc atccagtagc ttattagaac catttaataa
     1861 ttcttgactc ccatcagcag ctttattgat acctgttttc atagaaccca tatttttaaa
     1921 aactgattca gtataagtcc ctgtaataga ttctgctatc tcatctttta gtttattagc
     1981 tgctgtctca ctcatttttg aagctacaaa actacgcccc ttagtagttt tataagtaat
     2041 atttaacctt tctggttttt ctgtcatgag agttgtcgct tttctagaaa gatctttagg
     2101 aaaggtaata accatataat agtgaccctt ttctaattct ttttctgctc ttttagccgt
     2161 tgtaaaatgg aaatctaagc tatcattttt agacaaattg tcctccatat ctttgccaat
     2221 tgaaatagtt tttccattta atttagccga tttatcttgg ttaacgactg ccacaggtaa
     2281 atttttagta ttcccataag gatcccacat tgagcttaga aagataacat tatatagcgt
     2341 tggaatcagt gcaaccccag ccatcgtaat ccataatttt gggcttttta tgattgcttt
     2401 taattcgtct aacatttttt ctcctatttt ggacatattg ttcatttttt tgataaaata
     2461 tattatacag tgatgataga taatttcaag ataaaaatct cgtttttgga cactgtgttt
     2521 aaaaatgtac aaaaatatta aggggtaatt aatatggtaa acgatacgag gagagaaaaa
     2581 acaaaaagag ctattgaagc agctatgatt acgttattaa aagatcagag cttcgatgaa
     2641 atttcaacga ttaatctaac taaaacagct ggaatcagtc gttctagttt ttatactcat
     2701 tataaagata aatatgaaat gattgatcaa taccaacagt cactcttcaa taaagtcgaa
     2761 tatatttttg atcgcaatca atttaaaaaa gaagatgcct tgctcgaaat ttttcaattc
     2821 ttagatagag aatcattatt tgctgctctc ttaacccaaa atggaacaaa agaaattcag
     2881 acctacatcc ttaataaatt acaattaatg ctttctaaag aattacctga ggtcaatcca
     2941 gatgcaacaa agtctgatat caaccgtttg tattatagtg tctatctcag tcatgctatt
     3001 tttggagtct atcaaatgtg gataacacga ggaaaaaaag aaagccctca acaaattaca
     3061 caagtgctac tcagtttatt acctcaaact taaaaccagc cttccttata ggctggtcat
     3121 ttttagttag gtttatttta tcctcgttat aggataacaa cactaatatg gactgacatt
     3181 ataaatgacg atacatttga gaagacatat ctaaaaattg gcctacaagc tcgttatgtt
     3241 ctcctataaa atcaaagagc tgatcctcac tcagtacacc gcatttgaga ttattttctg
     3301 cttgttctga tagtctaaac cagtcttcgc taccataaaa ttctcttaat ttcacgtaat
     3361 cttcatagtg cttttgaaaa tcatctatag cttctactaa tgcattgatt ctaggtttga
     3421 agctatcata cttctcttcc atttcttcta tttttaatct atcatttaga ttcataataa
     3481 aatctcctta gttatataag attcgacgag ataagttatc attctcaagt atttgtaacg
     3541 ttaattctac tatatccttt ttacaaagta aaaaattact cattacaaac tgacagtgtt
     3601 gatttttatt ctaatgttag tttaaaatct ttttggttca ttaaatataa tgactcacta
     3661 aaaagtatat aacaaaaaaa gcctataaaa taggctttta aagtatgtaa tgtaataatt
     3721 aaagcatttt gttgtactgt atttttgaga cttccttaag gttgctttac aagtctttat
     3781 agctttaaat caacatttta aacttttttt gtcaacatta gttgacatta aaatttcctt
     3841 caaagttagc aaaaagtaag caaaaattta atttacaact ctgtcaatca atgagttcag
     3901 caggcaagaa ctagcaccca ataaaggtgc tttttagttt gctgtaaatc ttgcgttttt
     3961 attttttctt aaacagtttg atatagtctt gtttgctatg gataatatca gatacaaaaa
     4021 cggcttttct gtcttctaaa atgtgataaa aagctaggta gtcccctaaa acaatacaac
     4081 gagtattatg tggcgggtaa atagtttcac ctactctgtc atctgcgtta aatcctgctt
     4141 ctgggaaaaa ttcaagacgt tctagcccgt aaagaatatt gttgacggtg ttagcccctg
     4201 cctgctccga aaagtaagta gtttcaatat agctttttat ctcctgtaat tgttcttgta
     4261 cggtttcagg gataataagg ctatgggttt tattatcaga taccaaggtt agccctcact
     4321 tcactagcgt tgtatacccg cccatgctct aaatcgtcaa aacttttagc aatttcagct
     4381 cgtaaacctg caagtagttc agctcgttct ttatcggtgt cagtctcaaa aggtaaggct
     4441 ttattttgta caatgttttc taaaaacaaa ttaaagctag ctgtcatgtc aaggttttgg
     4501 gctgcgataa tagctttagc tttctctagt aaatcactgt tggttttaaa gttgacctgc
     4561 gtattttttt ctaaagcgtg catagtagca cctcctttta gttattataa cactttaaac
     4621 tatacctgta tagttgcata tatccaagag cctatgctgt tgttattaac acaccattcc
     4681 tataactctg ggcgaattca agcaaagctc ttttcttagt ctcgtagtac caactttgac
     4741 ttttgttaag ttctgctatg atgtcttgct gagttttttt ctcactaatc aagtaacact
     4801 caatcaatat ctttctgtag tctatcttag acagttggtt gatagcatac ttgatagcgt
     4861 ctagctcctc taaggcacat tctcggctta tttcaaggtg ttctctgcgc gtgggatgat
     4921 attttatatc aaactggtaa cgctcgttat agcttaaatc aaggctattg gcgatacgtt
     4981 gccatctatg aaactctttt aatttacgaa tagcgttctt cttgctcatc taatacctct
     5041 aaagcttctc tatgtaattt gaatgcagta ttccttgaat aacctagctt gtcgggtatc
     5101 tcatcccatg ataagtcatc cacgtatcta gctttgataa cagctatctg tttttccgaa
     5161 tgttatagcc agtatttgtc ccccttatcc atatagtaat ggaatctctt ccaatgatat
     5221 aagtatttag tcattctcat gtactttgga cgcttaggaa agtcatcata actataataa
     5281 ccatgtttgt gttttgcttt tgaatctact ttcagacact ctttaaaggc tagttgccaa
     5341 tagtattgac aatctgtctt acttcgattg aatgtcgctt gatgaagctt ctgacaagta
     5401 ccacaagcaa aggcatgaga agccttaaat aactttctac aacgtctctc acagtcagga
     5461 cacaggaaga agtagcgctt accaccataa gttcctggta tcgtttcaag taagaattct
     5521 tgcccctcat aatggacgat taggttatct aggtctatgg tgatagattg gtcatttatt
     5581 gttcctgtaa ctctagtctt ttcctgtttc ttcattggtt taatgatact ctcaatagct
     5641 agttctaaca tcttttctcc ttagtattca aataacccaa aactattgat aaacgacaaa
     5701 aagagggaaa tccctctgat tgtttattta accagtaact caccctcaat agccatatca
     5761 tacaaataat taaagccttg gctgatagat tcaagtacag ctcctaaatc ctcttgagtc
     5821 atctttttat aattcatgga aacgtgttcg gcgagttggt tgtggtctga gatgaaagcc
     5881 atgaatatgt cttgtttgtc cgctttctct tggctattgt caaataaagg aaccacgcgc
     5941 ttcttgtcag tactttcaac ctcctttagt aactcagttt cttgagctac gtcatcaagt
     6001 tcttcatccg tcatatcctc aggttggttg taatagtcct taaagctgtc acagatacgc
     6061 ttgaagacct tatttagttt tctgtcttcg gcatattcaa gtactaactg gttagcatga
     6121 ccaccttgat catcattgtg ataagttgcg tcaatcactg gttgctcata gctcccagtc
     6181 atatacccta gaatggcatg acaggcaact tgcgcagtat caaagtcttt aaaagtatag
     6241 tggaatgtaa atgtttttgg tgtgtctgaa aatgttctca tgttatttct cctttgtgat
     6301 tgctataatg tctgataaat tgatgatggc tacccagttt ggttgttgcc cagatagaag
     6361 atatttgacc aactcattat aaagggtgca gtctccttgt atagtgacgg tgttaccgcc
     6421 tcgtgtgtgt aattttagtt acctgtacaa gaccaataaa gaagttgtgg tactcatgtc
     6481 ttcataattg ccataagttg cttctgaaaa tttaatgtct atcacagata ccgatagggt
     6541 aaattgattg attcgatatt caaaatcatc cagtgattct ttgtgttttt gataaaatag
     6601 cttgattttc attgtgtttg ttctcctttc tcaacgggta tagcaaagcc acagtcaaag
     6661 acccagcgat aaccacggcg tatgagttct ggtcttgtaa aatcacaatc accaggttta
     6721 gacttggtgt tgaaattgat tttgctgatg aagtcataac cgcattgacc attgggaagc
     6781 tccaatcggt atagctggtc gtttggcagg tcatcataat ggtaatgcat tttctttatg
     6841 ccatgatttg tatagttagg attcatatgt ccctcttttc tctctaaaat gtgtgttatt
     6901 aacatttaaa aaatcaaata gcacagcaaa aaacacaggg ctaaagccag ttgtatcaag
     6961 ggtttttgac attttgtgtt tttagtagca ttaatttgac caaaagactt tctttttttg
     7021 cacgcgcact attagttata aatattatta tctatagtat taaatactac taatactaca
     7081 atagagtata aagcttataa taccaatgtt ttggcttgtg ttattacgtg tagtttttat
     7141 gtagtattag tgtagttttt aaactcgttc atagtaagat acgggagtgc cacctttaaa
     7201 tagtcttttt cttgcgggat tctctccttt tttccagccg tcatcattat ctaaatggtc
     7261 acgtattttt tgagaaataa gagctttacc gccttgcgtt ggtttttggt taaaccccag
     7321 ataagcaata tggttaggac ttgtgacctg gagtaagcaa tttgtctgtg cagaagggta
     7381 gtcactataa ctttgagcat tatctaaagg ttctcccaac tgttttagga cgtattgccg
     7441 ttgttcatac tgtgataaac tatcccaacc ttcaacaatt tgaaactcat tcagtaattg
     7501 gtcaatgatt tctttgtcaa cgtcttcaac tttataatct tcttgaatat cagctaactg
     7561 attcatcaac tctttagatg gtgttagtgg ttcataattg ttaaaccata ctttggcttc
     7621 agcgagtatc tgtaagaaat aatcttcttc cacctccata ggatgttttt ccacgtcatt
     7681 gataccacat tcaataggga agaagcgtct ttctgttcca ctatccttaa gaaaagattt
     7741 tttgttagct gtcccgataa agacacagtg ccttggatga ggagtggctt tacgttcata
     7801 aggttcacga taagtatcac tatctgagga aatgaagctt ttaaccgttt caatttctgc
     7861 ctttgacata ccttttagct cccctagctc gatgatggca ttggcttgta tcttctgata
     7921 atcactatca tttttaccaa acttgatttc tgaatcggtg tggtagctag gaagcaagcg
     7981 cttagtaaca gtgctttttc cagttccttg tcttttatca atgagaatag gaacgacttc
     8041 aaactttact ttacggagat aaattctagc tataagacct gttagccata ctttggcaat
     8101 ttctctatta taggaattat cagcacagcc taatagatca ataaagtagc gttctcctct
     8161 agctttacca tcccattttt gactttcaat acgctgttta atggggtggt aagagttctt
     8221 tttagctaaa gcggtaatag ctacctctat atgttcttta cgaggggtaa accgatattt
     8281 atcatcaatg aatgcaatac aaaggctggt ctgttcattc gtccatagcc ctttttcttt
     8341 agaccaaggg agtgttttag tgatttcaat agttttttca aattcgttgt atttaatacc
     8401 tgtgaagata ttttcgtaga attcaaaaac cttaccgaca ttgtaggggc tactaatgac
     8461 atattctttg tctcctctac cttttcgcgt cctgaatgca ggggcaaaag caggttgagt
     8521 ggcttgtgat agtttatttt gataatcttt caattcttcc ttttctatgg cttgattcct
     8581 ctctttctta gttctgtatc gagtatgctt ctaaaagttt tgtctatctc atcaatgggt
     8641 agtggcttag ttgtcacgct gttagctatt tgtaccagct cataagccgt ctctaaatca
     8701 caatccaccc atttattaaa tagcaaacca acaaatttag ttaaggctac attacgtccg
     8761 ccttcgtctc caaaaccatt aaacaaggta tctatgaccc tcatggtaat agaacgctga
     8821 cttctagggc gtgacgtgta agtagtagta acctgtcggt tgggtgcttt agggacagga
     8881 taatcaagac cacggttcac atagcgctga tagtcttctg ggtcgcctgt tgtgacgggt
     8941 aagccttgta attgcgacca ggtaagacta gctaaatcaa acggtagtcc aatcttatta
     9001 gctatctcct tgaccacttg tttatacgtc gttttgtcca tagtgtcact aggcttcacg
     9061 acaaggcgat agcgtggctt ctcaggggtg tgtttaatcg ttggataaat aatatagctg
     9121 tattcccaaa gtgtcttaga aacgatttta ggcaggttta cgtctgtttc tatctcgtca
     9181 tagtcaagaa aaatcaaatc acgatagact agactagcat tattgcgctt atagctaccg
     9241 tttttctctg gggttacctt gccactcaaa cagtacggag cttgtgttcg cttgtattct
     9301 tcaatatcga tagcctcagg cgttttcatt ggtttaaact gtgcgatata gtcaaacggt
     9361 tctaattgtc ctttgtaggg gtacaaataa gagctaaagc ctcttgcttc ataaatagcc
     9421 atctacacat ccacccccaa aaagataaga atatcactga ccttgtaata atgtttcctt
     9481 gtgtcttcta gtggtggttg gtatcgtttt aacccagcgt tttcccaccg ctttagggtt
     9541 ttacctttga tatttaattc ctctttgacc tgttcagccg tgatcaaccc taaaactctt
     9601 ggtttaggtt gctggtatac ttctaagaag cgattaaaag cggtcagatt ttgttctaag
     9661 agttttgctt cataatcttg actaaatacg ttcatactaa cctcctttga gtaattcctt
     9721 ataactggtt aaatcggcat tcaataagac acttaggcgt tgttgttcct tttgtacttg
     9781 attataaaag gctttagcac catctagtaa ttcttcttta ttagctggga taaagtaacc
     9841 acgattgaat ccgtgtctaa tgccaataat agggacgttg tagcgcgtga ttaagctact
     9901 tgatgatact ttggacggag cgttcttcgc gtttcagtat taagccaatc tctgccctgt
     9961 aatgggattg tctgccccaa ccttaatcag attaaggaca cgtctataat tttcaggcaa
    10021 tgtcattcag ttccccctaa ttgtaataat ggttctgtgc ttgaatataa gccccatagt
    10081 ttgcattctg acgtggttta ggtgcttggg tatcttctgg taagtcaatc tctattaatg
    10141 gcttagaacg gcttagaaga agccctatga gacctaaaac aatgaataaa ataagtgtct
    10201 gtgttagtgt aaagttaagt tcttgcatgg ttacaactcc tttctgtatt tatgtgtcat
    10261 cttatcatgg tcagaaataa ggcacttcaa agcgcgacaa ctcgtgttta taattgcact
    10321 agttgtttcc ttactatatt cttgagcagc caaatctagg acatttagaa tatccataag
    10381 ttgaccgcaa agaccttcat aatctactaa aatgtcgtta gcgatttcat ttaattcttc
    10441 aatactcctc atgcggatac ctcacttaaa taagtttcta attcccctgt gtctttctct
    10501 gaacatggta aaccgttaac ggctctaaag acaatctctg tggtttgttt ataacctaaa
    10561 gcgccccatg cttcttcaaa agtagtggca cttttcctga atgtagtcgt atactctgcc
    10621 attacattag caataatcac cctagcgata tgatggttat atagtcgagc aaaataggct
    10681 tccgctttat ctttgctgag ttggcgtttt ttgagcattt ctcgttgttc aggagtgtat
    10741 ctatcttttg aaaaaggatt tgtttctact ctatatttca ttatgttttt tctcgcttaa
    10801 ttattatttt ctgtgtaatt gcttgtttct tatactagat tcatgctagt tttatgatta
    10861 gttcatgtta gtgtataatt ctgcgaataa ctcgctaggg atacgctcta gcgctttttg
    10921 ttgtaagtgg atagctttaa ttctatcttg tgttttggtt ttaatgtctt ctataatttc
    10981 ggatgttgaa accacttgtt catagtaaat gctagcttta taaatgagtc cttgttcttt
    11041 taattccttg ttagccatct tttcaagagt aacttcatgt aatacttcaa cattgcgata
    11101 atgaccgttt ttaaggtcga atgttagcca ttttctacgc ttccaattat atagggtact
    11161 tctgcaaact tctgaattcc caaatccaag aaaagaagcg atttctgtta atgttttccc
    11221 ttcaatttca gatagtttat aagcgacatt tttaaatagt actctcggat ttcttttttt
    11281 cttaatcata ttgttttgat ttttgagcac acaaaaagcg cactcccttt ctgttttttg
    11341 tgttgactga aatagagtac gcatgtataa tatttacgta ctcactttgt gggtcgagcg
    11401 atagcatagt aaccaaaaat tggcgttggc ggttactgtg ctattttttt gtttagtctg
    11461 acaaatcttc aagggctttt ctgattgctt ctggctttgt taagccatga ttatcagcat
    11521 atgcttgtac cttttcatca agtccttggc taattctaac cgttatcttg atattgttcg
    11581 gttgctcgcc ctttggtggt ctgccgactg ctcttttgtc agtcattgta tacctcctta
    11641 gttttgacga cataattatt ataattaagt cgtcttaata agtcaacccc taaatcaaac
    11701 ttttttgcgt actctattca gttgttaaag gactaatttt ttaatatcgt tatattctgc
    11761 attgagctcc aataggacaa ttactttttg ttcaagtttt ctatatcgcg tcaactcatc
    11821 agcacttagg caatctaatg caatttcata acctacccgt gactgtttca gttgtttagc
    11881 agtctgtcct gtgacagatt ttagcaaaag gtcgctaata gccttgtagc tccattgatt
    11941 aaagtgttcc caatccttga tagcttgtgt taattcttta tgattagact tttcaagctc
    12001 tcgtatcagt ttaaagttag cgttttctct ttctagttct tcgatatggt cataaatcca
    12061 ttgacgaaag attttacctt tctccgtttt ggacagcata ccgatctcaa aaattcctcg
    12121 cttattgaat agtcgggtct cgtattgttt gccatcagta gctgacaatt tgtcagtaac
    12181 tgaaaatcgc ttttctttga ggtagggttg gcgctctatc atcttttcaa tagcatttcg
    12241 gctcttatat ccaaatctct gcgctaattg ttcaatcgtc acaaaaatat ttctattcct
    12301 gtccagataa aaatcaattt caagttctcc aaaagaactt tttacttgtt ttatgatatt
    12361 cactgtttta cctcgttatt tgattattgt tgttttgtcg gggagaactg caactgttaa
    12421 gtcgttgcct gctctgcggt agtatgatac aatggaagta tcaaatcttt tactaaaacc
    12481 cctttaataa tagcttgcct gctttattaa ttgagtttag ttatactagt taaaggctct
    12541 gctgattggt ctcggtaagc ctttttttgt tgttttcacg cgcatttttg cgcttttttt
    12601 aatttttatt gtagattgac tcagacacac tgatattcaa tccgaacttt tctttaattc
    12661 ccatgagttc cacagtatca tctaaaatca tttctctagc aactaacata tctggtgtca
    12721 tttctgattt tttaaccatt tttgaatatc caaacttgtt ggaaacggct ttatttgtga
    12781 ttgtgttagc tttaatcaag tctttcttag ataccctatc taagctatta gtcaatcttg
    12841 ccatagcttc tttttgatgt tccttatcca gcattctaaa gacctcaaag ccttctagac
    12901 cagttgcttg tcttaactgt ttaatggttt caaataccca aaacttaaat gattttgctt
    12961 ctggtttacg acttgaaaaa atagtgtcat aaattccaaa ctcgttaaca attagcattt
    13021 cttgttgacg tcctaaactg tctgcgacgt ggtcggttga aacgacctca tcaactaaac
    13081 gctctttcac acgttttggt tttaagtcta gcgcgtgaca aatatcagca agaactgccc
    13141 accattcgcc ttggtgctct acaaatcgga tatcatatcc attccatgtt tctgttctca
    13201 ataaattgtc ctttctttag taatcttcag caagccattc catgactttt tgataaatgc
    13261 cattttttac ttctccgcca ttacgaactt tgcgataagt cacttctgta actccaattt
    13321 cagcgcttgc tgatttagaa gttaattgtt tatcagcttg ttttcttcgg atagcttttg
    13381 cttgtgttga ggtaataagc aataatttca actcctttct ttgctaactt tttgttagtt
    13441 aacaggtttt attttagcta acttataatt cgttgtcaag aaggtgattc taaaaagtgt
    13501 aaaaaaagtt agcttagttt tctttttatg atataatcga cttgaggtaa taaattatga
    13561 atagattgaa agaattaaga gctaatcaaa aaaagactca gcaggatatg tctgagtttt
    13621 tgaatatgag tcgccgaggg tatcagaaaa tagagaatgg tgaaagccaa atcaaaccag
    13681 acaaagccca agccctagcg gattatttta acgtgtctgt ggggtatctg ttggggtata
    13741 gcgatgataa aaaagaccca atgctaacca gctttgaatt agctcgaaaa gatgatacag
    13801 gagagtattt tgatttacaa gacatggtta ctcttatgga tatatctatg ttgaaaaatg
    13861 tagatactcg cgataagata ctttcaaatt taaaagaata ttatgattat tatggtattc
    13921 gaatgaaaga agaacacgaa ataaaagaca gtgagaaatt caatgctttt ttagcagaat
    13981 ttcaagaaga tacaaacgac tatatcatca tgctgcttac gggtatagtt aaacttcgtg
    14041 atgacattcg tttcacctta ttggatattc tagctatgga aggtgagaaa ctagaaaccg
    14101 ttaaaaatgt tgtcaaattt atatctgaca atggtgacaa ctaacccacg cgccactatt
    14161 ttcaagggtt tcatctatat aaataaaacc atggtatctg tatttaaacg ctttatacaa
    14221 agaaatttat tgaatttatt gacggtgggg ataccccaaa tgtttttaaa aagcttattt
    14281 tttatttttc tttactaacg ccaaaagaaa gggaaagcat aataaatatt ttagagggac
    14341 tagcgggttt agatattgat tttccgcatt aactcacgcg catagaggag aaacaaacat
    14401 gaaaaagaga attattgttg tgctggtcgc attgctagct attggtggtg gttcagctta
    14461 ttatttccta agttacgccc ctcaccaaga ggctgtcaca aactttaata aagctgttca
    14521 gacagttaag tcaaagaata agacgctaaa aagtgagatt gccaaagctg aaaagctcgt
    14581 taagtcaaac cagcagccgc tagatgtaag gactttagac aacctaaaag cagcagtagc
    14641 aactgccaaa agtgacttaa gagaaattcc aaaggtagca gataaaaccg aagctatcaa
    14701 gaaacaaacc aagtcgctta atcaaccaat agattattca aaagctactc agtctttagc
    14761 tgatagttct ttagcatata caaatagtgt taagcaacta gcccaaataa ccaatcccag
    14821 tcaaagtttt gttgaggagc gcttaaaaga gattgataca gttacaggcg ttcagtcagt
    14881 gacagaaagc aatgacccta atggaaaact aaataaacaa ggtggttata ctgcctcaat
    14941 ctactttact gataaccagg taactgaagc tgtagaaggt aaggatgtgc tcgataaggg
    15001 gaccgatgca ggaggaaata ttgaggtttt taaaaccaaa gaagaagcag aaaaacgtaa
    15061 tatatacctg agtgcatttg acagtgcagg tatgtttaat ccaggttctc actatgtatt
    15121 tggaactgtt gttattagga catctcacta tctaacagca agccaacaga aatctttaac
    15181 acaaaaaata tatgaaaaat tgatagaaat taaatcaaaa aattctggtt ctaccaaaga
    15241 ctccaagaag aaagaagtta gcaaagataa tacaaaaaca agtacacaag aacagcctgc
    15301 accttcatca actacggaca aaagtcagga atcacagtca agcagtgaaa cttcaaatag
    15361 tggtggtaga ggtggcactc ggggtgtgga agattcaagt gatacatctt catcaagtga
    15421 aatgacggaa gaggaattaa attctgcaat taaggaaatc caagactcaa gagattacta
    15481 tgcaccgaat gaatttgaaa atcaggttgg tgacacaact agcagtgata atggagatag
    15541 tacttatcat ccatgagtta ttccctcaat atgaaacacc gtttactata tttctcttac
    15601 ttcgctctag cttcaagcca atttctcgga gttcgctctc tgttcacact cgaaattcca
    15661 tcatttttca ccaaataatt atctttaaca accttttagg agagccgaaa agtccgattt
    15721 ctgaatacta tatgctaaaa aagtctactg atcttagaag ctatcacaac ggaaaaaata
    15781 aattaataaa cgaccgatat atcaagtttt ttcaagttct ttaagtgaat ttaccgagcg
    15841 ttttaggact attgaaatag gttgacgtat tatgttggca cgtaccaaca tttagggact
    15901 acagcaacat gtcaacggag ttgccaaatc tctcaacgtt gaacgattca ggagcgtcag
    15961 ccttccacca taggaagcca taggttattc aagctgttct tgcttttggt catctggaag
    16021 ggtagcgatg aggtagaggg ctgaactacc taaattactc aacgttgaat aatttggaag
    16081 ttcttttgct actgtctgtg ttaattttgc ttataaatgt gatgagattg ctattctaaa
    16141 cgttttaatt catccaaaaa ttcacatctc ctttctagtt tttctccctg gggagggggg
    16201 tcgtgtgaga aaaaaagcga atatttggac agttgaccct tcccaccggt ttccaaactc
    16261 ggattgaaca acatttttta atgatggggg gtaaccttaa aggaatagcc atgaatgaat
    16321 tacaattttt gatatatacg gctaacaata gccaagagac agccagcttt tctgggctat
    16381 ctaaaaattt accaacttac taaaaccaaa caaaaaaaga cctcgcaagt tttccacgct
    16441 cgcaaggtct taaaaaacaa caatattata tcacaatttt ctatttataa tatttcggat
    16501 attaacccga taccgttatt ataccatgat ataaactaat ctaaaaccct tttaataata
    16561 gcttgcctgc tgatggaaag gtttataatc gtgaaaataa atgaagtaaa aaagaaagat
    16621 ggtacggtaa tttatcgtgc cagtgtttat cttggtactg ataaagtaac aggtaagaaa
    16681 gtaaccacta aaataacagg acgaactaag aaagaagtta gagaaaaagc taagcaagga
    16741 gctgttgagt ttataaaaaa tggttctact cgcttcaaag ctacttccgt aacaagctat
    16801 caggaacttg caaccttatg gtgggatagt tacaaacata ccgtaaaata caatactcag
    16861 ctagctactg aaaagctgtt aaccgttcat gtcataccaa tttttggagc atataagctt
    16921 gataagttaa cgacaccact tatacagtct atcatcaata aactagctga taaaactaat
    16981 aaaggagaaa gaaaagctta tctccattat gacagaatac acgcgctaaa taaacgtata
    17041 ctacaatatg gtgttatcat gcaagctata ccattcaacc ctgctcgtga ggtcattctc
    17101 cctcgcaaca ctaagaaagc taacactaaa agagtaaagc attttgaaaa tgatgagcta
    17161 agaacatttt tcaactactt aaacaatcta gataaaagta aatatagaaa cttctacgaa
    17221 gtcacacttt ataagttttt gttagctaca ggttgtcgca ttaatgaagc gttagctcta
    17281 aactggtcag atatcgactt ggataatgcc gttgttcata tcacaaaaac gctaaattac
    17341 aagcaagaaa ttaatagtcc aaagtcaaag tcaagctatc gtgatattga catagatagt
    17401 caaacaatta caatgcttaa gcagtataga cgaagacaga ttcaagaagc atggaagtta
    17461 ggtcgttcag aaacagtggt attttctgat tttatccata agtaccccaa caatagaacc
    17521 ttacaaactc gattaagaac acattttaaa agagcgaatg taccgaatat aggctttcat
    17581 ggttttcgtc acactcacgc tagtttattg ctcaacacag gtatccccta caaagaactc
    17641 caatataggt tagggcactc tactctatct atgactatgg atatttatag ccatttatca
    17701 aaagagaatg caaaaaaagc tgtctcattc tttgaaacag caattaactc aatatagcaa
    17761 aaaaatcaaa agttagcaaa aaagtaagca aacccagaaa tcaatgtttt aagataagag
    17821 aaaaagccca ctgttgtagg ctttcagtaa gtttaatctt aaaattaaag cattttgttg
    17881 tagaattcaa cgacaagtgc ttcattgatt tctgggttaa tttcatcgcg ttctggaagg
    17941 cgagtcaatg aaccttcaag tttttcagca tcaaatgata cgaaagctgg acgtcctaaa
    18001 gtagcttcaa cagcttcaag gatagcaggt actttcattg atttttcacg aactgaaata
    18061 acttgtcctg gagtaacgcg gtatgatggg atatcaacac gtttaccatc aacaaggata
    18121 tgaccatggt taacaaattg acgagcttgg cgacgagttg ttgcaagtcc taaacgataa
    18181 acaacgttat caagacgacg ctctaaaaga accataaagt tgaaaccaag agttccttct
    18241 tttgctttag tagcttgtac gaacaagtta cggaattgtt tttcaccaag tccgtatgaa
    18301 aaacgaagtt tttgtttctc agctaactgc aagccatatt ctgaaagctt gctacggtta
    18361 ttaggaccgt gttgaccagg tacgtagtta cgacgtgcca attctttacc tgtgcctgta
    18421 agtgataaac caaggcgacg tgattgtttc catgatggac cagtataacg tgacatctaa
    18481 atgtcctcct ctaaaataat tgaggaaata acgatttaaa aaatcctgat tcgtgcaggg
    18541 gattttcgcc taaacagcaa agggtactag attagttaac cacctgttga cgagcttcat
    18601 atttttctgc tgttatttca cacaagctat attatatcat gaaaataata ttgaatcaag
    18661 tttttatgtc tcttaaacct taaatacaaa tagacatcta ataagtattt ccgattataa
    18721 taagaaagat tcttaattct aaccaccaat agagaattag taaatttttt tatagaaaca
    18781 aaaaacctca gtcgaggttt tcatactatt ctgctgactc gtcatcaaaa taacgaatta
    18841 aagttttctc agttaaaata tctgaataag tgtaagactc tacataagta ttatcaattg
    18901 ctcctggtac agctgcagta tccttatact ctacgataaa caaagatggg taaacttcaa
    18961 ttaggcgccc aattttattt ttttccctct tacgaccatt ttcgagagta agttcgacca
    19021 tctgtccctc atgtgacttg atatcttctt ttattttttt catttttgca acatctgcaa
    19081 atgcatcact catattaaca tctcctattc ttcaaattga gctatactag aaaatttatt
    19141 atattccttt tgaaacatta attttactgt cccacgcgca ccagcacggt tcttttctaa
    19201 aataacctca acggtattat cttctacaat ttcctctgct tcttcgccct ctctacgata
    19261 ataatcatct cgatataaga aagcaacaat gtcagcatct tgttcaatcg atccagattc
    19321 acgaatatca gataagaccg gtcgtttatc ctgcctttgc tcaaccccac gagagagctg
    19381 gcttaatgcg ataacaggaa cctttaattc tttagctaaa atcttcaact gccttgaaat
    19441 ttcagaaact tcttgctgac gattttctgg tctagttcct gaaattaatt ggaggtagtc
    19501 aatgacgatt aaacccaaac catcatcaac ttcttgagat aacttcctag agcgtgctct
    19561 aatttctgta attttaatcc cgggcgtatc atcaatataa attggcgcat ccgccaaagc
    19621 tccttgagca atcgttacat tattccaatc ttgatccgtc aactgaccag ttcgtaaact
    19681 atgagaatca accattccct ctgctgccaa catacggtca actaaactct cagcccccat
    19741 ttcaagtgaa aaaatagcta caggtctatt ttgtttagtt ccaacatttt gagcaatatt
    19801 taaaacaaag gctgttttac ctactgcagg acgagccgct aagatgatta attgatctgg
    19861 atgtaaacct gtagtaatcc tgtctaaatc tctaaatcca gttggtaaac ctgtgacatc
    19921 tgaggtttgc tgtgaacgta attctaagtt ttcgtaatta acttttaaaa catcagatat
    19981 ctttctaaac ccgctacgat tactgtgttc gttaatatca actaaagcct tctcagcggt
    20041 agcaataatt tcatcagaat catttccttc ataggccatg ttgacagtat ctgtcaactt
    20101 agaaatgata tctcgcaaca tggctttctc agaaactatt ttggcataaa actccgcatt
    20161 agcacttgtt ggaacactat taactaattc aacaatatag cccaacccac caatgttttg
    20221 aagatcacct tggtcatcta aaatatttct aacagtagca gcatcaattg catcatttct
    20281 atcagctaag gtaatcatcg ctctgaagat aactttgtgt gaatatttat aaaagtcatc
    20341 tggacttata aattctctca ccataatcag tttctctggt gagataaaga tagatcctaa
    20401 aacggcctgt tctgccaaca ggtcttgagg ttgtactctt agctcagata cttctgccaa
    20461 acttcattcc tcctgacttg tcaaccttta tcatctctca gtttacgctt ctttgatatc
    20521 aagcttaatc tgtgaagaaa cctgtttatg taattttact ggtacttcaa ccttaccaat
    20581 cgctctaata gtatgatcta aatcaatatg acgtttgtca attttaatgc cgtattgttt
    20641 ttgtaactct tcagcaatct ttttggctgt gatagaacca aacgtacgac catctggacc
    20701 taccttttca ataaattgca ctcgtgtagt ttcggattct aattgtgttt tcagctcctg
    20761 tgcctgtgct aaaatttctg cttgcgcttt ttcttcggac ttttgttttc cttttaattc
    20821 accgatagct tgtgtcgttg cttctttagc tagattcttt tttaataaga aattttgagc
    20881 ataaccagta ggtacttcct taacctcacc tttttttcct ttccccttaa catcttgtaa
    20941 aaaaataact ttcatctcaa ttaatccctt ctattttcga ctgtactatt ttctcttaaa
    21001 tcttcgtcaa ttatttccag taacatacgt ctaacttgct ttacagactt gtcttgtatt
    21061 tgacaagcag caaaactaaa atgtccgcca ccaccaagtt tttccataat gcgctggaca
    21121 ttgatattat ttctactgcg cgctgaaata ccaatatttg aactgttttt agtaataaca
    21181 aaagtagcct caatacctgc cattgtcaac atggtgtcag ctgctttact agctataaca
    21241 tgcgagtaaa ctttatgttc ttctccggta gcaacaatga tgttatcata aatacgctca
    21301 ccttttaaaa tcaattcatt aattaaacga tattcatcaa aatctgtagc tgatattttt
    21361 tgaatagcca tactattact acctaaaccg cgtaaatagc ttgccacatc aaaagttcta
    21421 ctcgttacat tagatgcaaa atttcttgta tcaagcatga taccagccat taaaatactt
    21481 gcttgactgc ggcttaattt atctttagct tgttgaaatt gaatcaattc tgtaactaat
    21541 tcgctggcac tacttgcgcc actttctata aatgaaagga cagcattttt gggaaaatct
    21601 tcatctcttc tatgatgatc taccacaata acatctgcaa acttgttaaa gaattcctta
    21661 gataaggtta aagctgtttt agaatggtct accatgacta ataaagaatt ttgagtaatt
    21721 aattcaaaag ctctctcaac acttacgaga cgtgtttcgc cgtcttcttg caagtaatca
    21781 attgctcttt caatatcaga attcatgtca tttgggtcat aaactacata actggcatta
    21841 acaatattac tagcaaaaaa ttgcatccct acagatgcac ctagtgcatc catgtccaat
    21901 ttcctgtgtc ccacaataaa gacactatca actactttca aacggtcaga aatagcagtc
    21961 atcatagctc tagtacgtgt tcgtgaacgt ttaatagttg aaacagcacc accaccaaag
    22021 taaagtgctt ttttactact atcattttca cgtactacta tttgatctcc tccgcgaacg
    22081 agtgctgtat taagattttc taaagcaatt tgcccaattt gattatggtt accatcacca
    22141 taagaaattc ccatacttaa tgttaatgat aaatggtttt cctgagcgcg ttttcgaaac
    22201 tcatttaaaa tatcaaattt atcttttatc aatgtattta aaacactata gtcggtaaaa
    22261 atatagtatc gatccatatt gacacgacga taaaaaatat ttttagactg ggtaaaatca
    22321 gaaataaaac tagtcacgaa ggcgttaatc tttgacatat cagcctctaa catagtatcc
    22381 ataatatcat cgtagttatc aatggaaata ataccaataa ctggacgtag cattgaacta
    22441 tcatagtttc ttctattccc catgaaatta tcgaagaaat agaaaacacc cgtctctgta
    22501 tccaaataag ctgaatattt gttatcaccg tattcaaatg attgcccagc cgttccatta
    22561 cgtctactag taatgatgtc tttaacagta gcagactgaa aatgaccatt atccccagta
    22621 aaaatcaatt ctgcataagg gttaaaccac tcaatatcat ttgtttcaag atcaaattgt
    22681 ataacaccaa ctggcatttg ttcaagaaga ctcttaagag aaacttctgt ttgatcatta
    22741 agtaattcaa tttgttcaac ttcagatagt tcataagtta tcttttggta atataataag
    22801 gcaacaacaa aagataaagc aacaaaaata gccaataata gagccgtata ggattgaaat
    22861 aacctaacac aaatagctaa tagtccaaat aaaatcaggc ctattaaaac taaatggacg
    22921 gttgcaaaac gaaatctttt cattaaataa cctcttaaga cctaattata acacaaaacg
    22981 cccctgataa acaggggcga acactattat caattgatat ttttacgacc tttttgtctt
    23041 ccttcaaggt aaaccattaa aatactaata tcggctggat taactcctga aatacgacta
    23101 gcctgaccta gcgtttctgg gttaattttt ttgaattttt gtctagcttc tgttgcgata
    23161 gaatcaatgt catcccagtc aatatgaggg ggaatacgtt tttcttccat tcgtttcatt
    23221 tttgcaactt gatctaaagc ttttttaata tagccttcgt atttaatttc agtttccaaa
    23281 agttcaataa ccttactgtc taatggctca tcagtacaac caacaaaatc cgttgctact
    23341 gcatatgtga tttgtgggcg gcgcataaat tctttggcag ttaaagcatc tgtcaaaggt
    23401 ttaaaaccca attcttgaat ccgtttattt gtctcagaaa ctggctttaa ctttatagaa
    23461 tctaaacgtt ctaattcatt ttcaaattgc atttgacgct ttttaaatat cgcataacgc
    23521 tcttcatcta ctaggccaat ctcataaccg atttctgtta aacgcatatc tgcattgtca
    23581 tgacgaagaa tcagacgata ttctgcgcga cttgtcaaca gacgatatgg ttctagagtc
    23641 cctttggtaa ctaaatcatc aatcatgaca ccaatgtaag catcacttcg ttttaaaata
    23701 agttctggct tcccttgaac tttgagagca gcgttgatcc cagcaaccaa cccttgacca
    23761 gcagcttctt cataacctga agtaccatta gtctgtcctg cagtaaacaa accagcaatt
    23821 acttttgttt cgagtgttgc tcttaattga tgaggcaata caatatcata ctcaatagca
    23881 tagcctgtac gcatcatttc agcattttca agccctttta tagaacgtaa taaatcaact
    23941 tgaacatcct caggaagact cgttgatagc ccttgaacat aaacttcttc agtgtgtcta
    24001 ccttctggtt ctaagaagag ttggtggcgc tctttatcag caaaacgcac aattttatct
    24061 tcaattgacg gacaatatcg aggaccaact cccttaacga ttccagaaaa cataggagca
    24121 cggtgtagat tttgattgat gatgtcatga ctcaaagtat tcgtataggt caaccagcac
    24181 ggtacctggt cagtaatata atcttcatct ctagacataa atgaaaaatg atttggttgc
    24241 tcatccccag gttgtatctc tgtctcttca taattaatag aggaggcttt tacacgagga
    24301 ggtgttccag ttttgaaacg accaatttct aatcctaggt ctcttaaatt atctgccaat
    24361 gtcacagaag ctaaactatt attaggaccc gaagagtact taagctcacc taatataatc
    24421 tctcctcgaa gagcagtacc tgttgttata acaacagatt ttgctgaaaa tttctgatta
    24481 gttgctgtac gaacccccac aacctttcca tcctcaacaa gtatttcatc aatcatagct
    24541 tgtcttaagg taaggttttc ttgcttttct acagtttgct tcattgtttg tgcatagagg
    24601 gctttatcag cttgtgctct taaggcacgg acagcaggcc ccttacctgt attaagcatt
    24661 ttcatttgga tataggtttt atcaatgttt ttacccattt caccacctaa agcgtcaatc
    24721 tctctgacaa cgataccttt agcagagcca cctatagaag gattacaagg cataaatgcc
    24781 aacatttcta agttgatagt agctagaagt gtcttacatc ccatacgact agcagctaga
    24841 ctagcttcca cacctgcatg acccgctcca acaacaataa tatcatagtt ttctgcaaaa
    24901 ttatgtgtca ttttattcct ttccaatttc aaagtctaat tcattaaaac acaaaaatag
    24961 ccaaaactct cgaaaattgt acgtcaaaaa gacttacaaa ttccatgagc tttgaccatc
    25021 atagttattg ttaaatttat tttagcattt atcttttaaa agtcaaccag aggggacaga
    25081 ctatgttaaa tgaaaaccaa tttttatgcc tgttattagc atctctatcc cgatagtcat
    25141 caaaataagt cccatcattt tggtgattat agtcattgca ttttttccaa gaaatttagt
    25201 aatttcgttt gcacttatta aaataacata cattatcaca catagtatgg caaaagcaag
    25261 tatagtaatg agtttgccag ataaaccacc acttgataaa cccatagcag ttgcaatagt
    25321 acccggacct gccaaaaggg gcattgctaa tggtgaaact gccacattca ttggatcatc
    25381 cttatgctcc tctaggtttt tattggttgg tgaatgtata ccattaatca ttttataacc
    25441 tataatgccg accaatatcc caccagatat ttttagggcg gctagggtaa tcccaaataa
    25501 gttaaaaagt aagtggccac ttaatacaaa gattacaata atgacaaaag caagtaatac
    25561 tccttttgct gcaattcgac gtgaaatctt ctgatcatca tcagctacta aagccataaa
    25621 agctggcaaa ttagaaatgg gattcataat ggcgaaaaaa gccatgaaag ctaaaataaa
    25681 ttttgaaatc atacgattac ctcacttaat aattaagtgt caattaaatg tattgacaga
    25741 ttttatcatc acgataagcc tgatcaatca tcccaccacc taaacattct tgttcattat
    25801 aaaaaacaac agcttgacct ggtgtgattg ctcgttgtaa atcatcaaac acaacccttg
    25861 cttgattacc ttttacgtaa actgtaactt tactgtcagg ttgacgatat ctgaacttag
    25921 ccgtacattc tagcttaaat tcattaggca tatcacgtgt aaagtggatt tcagaagctg
    25981 tcaagctagt tgacatcaat gagtcatgat agaaaccttg accgacataa agaatatttt
    26041 tacttaaatc ctttccaact acaaaccaag gtttattatc tccaccatgt tgcccaccaa
    26101 taccaaggcc ccctctttga ccaatagtat aatacattaa accagcatgt tcacccatgt
    26161 cacgaccatc aacagtcatc atacgacctg gttgagccgg taaatattga cccaggaaat
    26221 ccttgaaatt tttttctcca ataaaacaaa taccagtaga gtcttttttc ttagcggtcg
    26281 caagtcctgc ttcttcagct atacggcgaa cttctggttt ttgtaaatga ccaagtggaa
    26341 ataacgtttt ttgcaattgc tcctgagata attgacttag aaaataagtt tgatccttat
    26401 tattatctac tcctctgagc atatgaacta ttccgttttt atcacgtgtt acttgtgcat
    26461 aatgacctgt tgctacatag tcagcaccta atgtcatcgc ataatctaga aatgctttaa
    26521 atttaatttc cttattacac ataacatctg ggtttggggt acgacctgct cgatattctg
    26581 ctaaaaaata ttcgaaaacc ctgtcccaat actctttttc aaagttgaca gagtagtatg
    26641 gtatgccgat ctgatcagca acagcagcaa catctttata atcttcagta gccgtacaaa
    26701 caccaaattc atctgtgtca tcccaatttt tcataaaaac accaataaca tcataacctt
    26761 gctcttttaa aagtagggca gttactgatg aatcaacacc tccactcatg cctacaacga
    26821 cacgaatgtt tgaattatca gtcataagaa ttctcccatc aaaaagtttt atttttaact
    26881 taggcttgaa gggccaagtt gttcttagat caatcatatt gactaagaaa cactataaaa
    26941 gtgcttatat attatagcac tatttgttgt caagttgtat gtttttgtta tgataaatat
    27001 ttattacatt gcagtttttt tatcttttta tgatattttt tagaaataat ctgttataat
    27061 gagaatgtac aaaaatcacg aggaaaaact atgaaacatt taaaatttca atcggtcttc
    27121 gacattatcg gtcctgttat gattggacca tcaagtagtc atactgcagg agctgtccgc
    27181 attggcaaag ttgtccattc tatttttggt gaacctagtg aagtaacctt tcatttatac
    27241 aattcttttg ctaaaactta ccaaggacac ggtactgata aagcattggt tgcagggatt
    27301 ctaggaatgg atacagataa tccagatatt aaaaactctc ttgaaattgc tcatcaaaaa
    27361 ggaattaaga tttactggga tatcctaaaa gatagtaatt caccacatcc aaatactgcg
    27421 aaaataactg ttaaaaatgg ggataggagc atgagtatta caggagtgtc aattggtggc
    27481 ggaaacatcc aagttactga gttgaatgga ttctcagttt cattaacaat gaatactcct
    27541 actttaatta tcgttcatca agatatacct ggcatgattg ctaaagtaac tgatatttta
    27601 tctgacttta acattaatat tgctcaaatg aatgtaacac gcgaaagtgc aggagaaaaa
    27661 gctatcatga ttatagaagt agattcacga gattgtcaac aagctgtcaa aaagatagaa
    27721 gctattcctc atttacacaa tgtcaacttc tttgattaag agaaagggta actatgtttt
    27781 atacaattga agagctggta gagcaagcta atagccaaca taagggtaac atagcagagc
    27841 tcatgatcca aacggaaatt gaaatgactg gtagaagtcg tgaagaaatt cgttatatta
    27901 tgtcccgaaa ccttgaggtt atgaaagctt ctgttattga tggattgacc cctagtaaat
    27961 caatcagtgg tttaacaggc ggtgatgctg tcaagatgga tcaatattta caatcaggaa
    28021 aaactatttc agataccaca atcctagctg ccgttaggaa tgctatggct gttaatgagt
    28081 taaatgctaa gatgggactg gtctgtgcaa caccaactgc aggtagtgca ggatgtttac
    28141 cagctgtgat ttctacagcc attgaaaagc ttaatttaac agaagaagag caacttaatt
    28201 ttctatttac agccggcgca tttggtcttg tcattggtaa taatgcctct atctcaggtg
    28261 cagaaggagg ttgccaagct gaagttgggt cagctagtgc tatggctgcg gctgctttag
    28321 ttatggctgc tggaggtact cctttccaag ctagccaagc tatagcattt gttattaaaa
    28381 atatgcttgg acttatctgt gaccctgttg caggtttagt tgaagtccct tgtgtgaagc
    28441 ggaatgctct tggatcaagt tttgcacttg ttgctgctga tatggccttg gctggtattg
    28501 aatcgcaaat tccagtagat gaagttattg atgcaatgta tcaagttgga tcaagtttac
    28561 cgactgcttt tcgtgaaact gcagaaggag gacttgctgc cacgccgaca ggaagacgtt
    28621 atagcaaaga aatttttggg gaataaagtt tatcaatttt ctaatctttc ctaaaaataa
    28681 attaaaacgc tacagtaaag ctgtaacgtt ttaatttact tgttttagca atttgtaatt
    28741 attgttgaca atagtgtaaa gttaatttat agctattcac tagtagcccc aagccgataa
    28801 accttgagca cgataagctt taatagctga attaacttga tcctgaactg tagctgttga
    28861 accccaacct ggcatcgttt ggaaaagtcc tgaagctcct gaggcattag caacgttagg
    28921 attaccattt gattcacggg caataatatg ttcccaagta gactgaggaa ctcccgttgc
    28981 agcagccatt tgtgctgctg ctgctgagcc aatagcccct gcagtatttc cattgctcaa
    29041 tacttggcca ctcggctggt gttgagcagg tctataagtt gtctcagtaa cagcataagc
    29101 ttgttgtgct tgactggtag caggggtatt ttctgttaca actgcttgtt ctacagctgc
    29161 ctcttcactc gcagtaactt gttgctgaga attagcttct gtagattgag aacttggttt
    29221 tggagcctgt tttacacctt ctacttttga tttagatgtt gccttattca tttttgattt
    29281 tttggctacg cgaactttat ctgcttttga caaagattca gtagtaactt gtgaactttt
    29341 aaagatgacc gttacagcca aaatacatga aaaaagggct actaaagtga tagctccgta
    29401 agctaaatgt tgttttttta cattcatttt tgataatttt cttcttttat tcatgtcacc
    29461 tatattatac tttatttgtt acttaacaat tgaatagatg ttaaaaatat tacaaagaga
    29521 tttaatttta agtaaatctc tttgttttat caacttttat taataccaac cgttactatt
    29581 ccaaaatgat agcgctgccg accaagatcc gtaacgagaa gccacataat tgtccgctac
    29641 tttttcttga ttttcaggag ataagtcgcc atttaggtaa gattgagaca gttgatatct
    29701 tccataatat tgtccattct gtgcagtata actaccattt gattcacgac gagctatttc
    29761 ttcttttgcg gctgaatcac ttgaactcaa atttgatgaa gaattcattg aattttcaat
    29821 tgttggttga gattttgctt ctgcttgact agttgtagaa ttatccaatt ttaaaacatc
    29881 acctatactg atgacgtcag cgttactgat actattgaga gacactaact cttgtaccgt
    29941 agttttatga tttttagcaa tagctgataa ggtatcacct gatttcacgg tataagatgc
    30001 tgcgcttact gaaagtggta aagctaatga agcacccact aaaagacctg ccactcctaa
    30061 tttaaatggt tttgattttt taaccgaggt tattgacata atatatgctc tctttcatta
    30121 attgatacgc gtactatcat accttgtaaa tattactaat tgttttgcct tagatgaaat
    30181 ttattacaaa taaattgtaa attaataaag aaaaggcctc caaaaaataa tttttagttt
    30241 tctttaagta aagttgttga gcttttcaca ttatataaat gaaaaggcta agaccataaa
    30301 ctgatcttaa ccttttaatt ttttaataga aacaaactca aacccaataa taacataagt
    30361 aatatactaa aagtatcccg aacagtccac ttcaataaac ggtacttact acgattggct
    30421 ccgccttgat aacctcttgc ttccatagca attgccaacg catctgctct tttaaaacta
    30481 gaagcaaata atggaatcaa aatagggata atggacttaa ccttatgaat taggtttccc
    30541 tctccaaaat caactccacg agctctttga gcattcatga tacgtgttgt atcatccatt
    30601 aaagtaggaa cgaaacgtaa gctaagggat aacatcaaac caatctcgtg tgctggtact
    30661 ctcagtactt ccaatggttt taacaaagat tcaacagcat ctgctaaact taacggagta
    30721 gtagttaagg taagtaatgt cgaaaagaag ataattaaaa caaatctcat aaaaataagt
    30781 gctgcttgtt gtaacccaag gctagtgatg ctaaaaatcc aaaacgagaa gataacctgc
    30841 ccgccttgtg caaacagcat ctggaatagg gtggtaaata aaataatacc aaccatcggt
    30901 ttaataccat tgaaaaaata ggaaaatttt atctgagata aacccacaat aaccaacgta
    30961 aaaatgaata cgatcacatt tgtcactaca ttatttgccc aaaaaacaat aatgataaaa
    31021 agtaacatgg ctagtaactt acttcgagga tctaatttat gaatgagaga attacctggt
    31081 atgtaacgtc ccaaaatcaa tttatccatg cttaatagcc tccacaaatt cgttaatagt
    31141 aattggtaaa ctaggtaaat ttaatccctt atgagatagt ctttgagcaa acttggtgat
    31201 tttgggaact cctaattgtt tactttctaa aagttctact tcttgaaaaa tctgttttgg
    31261 ttgtcctgat aaggttactt tccctgcttc taaaacatac acatagtcag cataatccgc
    31321 tacatcgtcc attaagtgag tcactaagac gatagtcatt ccttttttat gaagattttt
    31381 aaaaagagtc attaattctt ttcttccctt aggatcaagt ccagctgttg gctcatccag
    31441 tactagtact ttgggttcca tcgctaaaat accagctata gcaacccgcc tcatctgccc
    31501 tccagaaagt tcaaatggat ttttatcgaa taaatcctca ctaataccaa ctaaccttaa
    31561 tttttcttca gccagccttt cagcttcaat ctgagaaata ccaaaatttt gtggtccaaa
    31621 agcaacatcc tttaaaactg tctcttcaaa aagctgactt tctggaaatt gaaaaactaa
    31681 accaactttt tgccttataa atttgatttc tttgttcttg tcccctgctt taatagaaaa
    31741 atcatcgaca attacctcac cttttgtagg aatatgtaaa ccattcaaaa gttgcataat
    31801 agttgatttt ccagaacctg tgtgcccaat gaatgcggta taggaagcat cttcaatttt
    31861 cagattaacg tcaaaaaggg cacgcccttc aaaaggagtg ccggcttgat aggtataact
    31921 tacattttta aattcaattc ccataattgt tcttctaatt ccttctctgt aagatactct
    31981 gtccctatat caagaccatt agcagccagt gctgccatta acctacttgt aaagggaaca
    32041 tctaaaccta aactaatcaa acgatttcca cgaccaaata acgcttttgg agtggatgtt
    32101 gactcaactt taccattttt cataacgatg acacgatcac tcaatgcaac ttcatctaag
    32161 tcgtgtgtaa ttgaaataac agtaagatta tatttttgac gaatagctcg tatcgttctt
    32221 atcagttcca atcgtccctc aggatcaagc atactagtcg cctcatccaa gatgataact
    32281 tgtggacgca tagctacagc tcctgcaatg gctacacgct gcttttgccc tccagataaa
    32341 cgtgcaggct ccctcatttt aaattcagac atgcctacca aatcaagtgc ttggcccact
    32401 ctttctttca tgtctttcaa aggaatacct ttattttcca aaccaaaggc gacgtcatct
    32461 tcaacagttg cccctacaaa ttggttatca ggattttgaa ataccatgcc aatcttgtga
    32521 cgtaactccc agacattatc ttcggttaac tcttgtccat caatgataat ctggcctgac
    32581 tccgcctcta aaagcccatc tattaaacga acagtagtgg atttcccaga accattatga
    32641 ccgataatag atagccattc tccttgtttc acatgaaacg atacattctc caattgataa
    32701 tgtgtttgat tactatcata tttaaaaaag agattattaa cagtaataat atttgtcatt
    32761 atttaaaagt atccttaaat aagaagcctg cacctttaaa atagtcgtag cctgaataca
    32821 aggtaaagaa taaagcaatg tataacatga tgttaccaag tgtcatccaa tgtactaaca
    32881 aaaaaataac tgcaaacatt tgagtagccg tctttatttt ccctggcata gctgcagcaa
    32941 gtactgttcc acctgtttca actaaaagta aacgcaaacc tgtaactgct aactctctac
    33001 aaatgataat agctgaaacc caagccggtg ctaagcctag tccaactaac atgataaaag
    33061 cactcataac tagcatttta tcagctaatg ggtctgcaaa tttcccaaaa tttgttacta
    33121 cttgccattt acgtgctaga taaccgtcta ggtaatcggt caagcttgca atagcaaata
    33181 caatagctgc aacaatatgc caagttgtac ttgttgttac tgaagttaat actataaata
    33241 acggaatcat taaaattctg actacagtta atagattcgg aatattttct ttcttcacca
    33301 taaattctcc ttttggaaac atagtattcc tatattatgt tatttcaaaa ctagtaaaag
    33361 acttttgaca taccaacttt atagatgtct aagcttcctt attatatcaa aatttcagat
    33421 tatttacgaa gattttggaa aaataacgaa gtcagatgtg tcagcattat ctaagaaatc
    33481 cttaccaatc gtaatgacat cttccaagtc taattcttca acgatagatg gcaaatctaa
    33541 atacatcgtt tttccatcat ataaataggt gacgaattgc attgcgagat tttcaataga
    33601 atctaagctc cttaaaaaat cgccatagag ctctctctta acgttttgta agtggctttc
    33661 ggtaagatca gatgattgca aagcattttt tagcaataac ctcaactgag ttgaaaaagc
    33721 aattggctct gtcgtatcta aagaaataat aacgcactca aaatctggat gaacttcaat
    33781 ttctatatca aaagagtcat caatttgacc agattcgtac cagtcttgat taattgtaga
    33841 agtccatcct aaaagcattg caaaaaatag ctgtaatccg attttctctt ttaggtaaga
    33901 accatgtctc atatgacttg attttcgata accaatagct aatttaggtt ttgtcactgt
    33961 catatggcaa gagttgtttt ttataacagg ttcaagacta atagaatctc ggtcaatatt
    34021 ctgtgagtaa tttgttaaat gagaattttt ttgaaggtaa gttataattt cttgtggact
    34081 aaattgtcca acaagaacta aattcatatt agacaactga taaaagtctt tgtgattttc
    34141 ttgtaaatcg gttaaggtta tttgagagat tgattggtaa tctccagcga tatcaaatgc
    34201 taatggagag ttggggtaaa ggttcgataa cactccttga taaagcctat attctgggtc
    34261 atcttgatac atttcaattt cttgtttgat aatgtctttt tctttagtaa ttgactcttc
    34321 tgtaaagttt gttgtcaata caaagtcaag tagtatatct aagcagtttg taatgtgact
    34381 tatagttgaa aaataaaaac tagttttatc aaaagttgta aaggcattac tctcggcgcc
    34441 atatttagta aattgagttg cagcgtcttg tcctttatcc agttcaaata atttatgttc
    34501 taagaagtga gcaattcctg ccggataatg ttctacacaa ccattcctcg tatatcttgt
    34561 atggagtgat ccaaaatttg ctgtcaatac tccaacagtt tccttaaatg atggttttgg
    34621 aataaggtat acatttagcc cgctctctaa tgttaactta tatacttcct cctgaaggtt
    34681 ctgataagtt attttattaa ctgtcatcct agtttccttc caaaaaataa aaagcttgta
    34741 attttaattt tgttgctacg ctgacaatat ctgattttgt tacatcgtcc actttgtcaa
    34801 tgaatagtgc tgaagtatga aactgatcta aataatgttc atgataaatg tggtcaacta
    34861 tgaccttagg attatcagaa gctaaaacat agttcatttt caatatatct tttgtttgtt
    34921 tcaataatgt agaagagaac ctgcccatct taatattatt gaattgctta ttaataagct
    34981 tcaaaaaacg ctcacgattc tctttatcaa tacctgcata tatcgtaaag agacctgtaa
    35041 agctgtcaaa ttgactacct atagtatagg ctaaaccttg cttttctcga atttctgtaa
    35101 acaacaagga atgggcaaaa gcaccaaata aaccgttaaa tactattaaa gcaaaataat
    35161 cttcatcttt ataagttatt ggaagatgat aagccaattg cataatagat tgattaacgt
    35221 ccttatcttc tacttgactt ctcgtgatat tttcataagt ctgcgtataa tcaaaagcta
    35281 atactttgtg acgtggctca aatgccatac gattaaatgc ctgaatcatt cgatagtcat
    35341 caaaatctcc tacgacaaaa atatctaatt gatcttcctt aagcatcttc tgaaattctt
    35401 gataagctgt aaatgaattt tctgattcca ctaaagatgc tgtaccatac ttaggtaaac
    35461 gaagtgtctt attattgtag aataaagatt ttagtgctaa gtcactgcta taaaaattat
    35521 cttcaatatc ggcctctaaa tattgaataa ggtttttctt ctcagtatca aaaatagatg
    35581 tttgatactg ttctaaagaa attaaaggag aaaacagcat atcctccaag aatgtaataa
    35641 tttgttcaac gatattttct tgttctaaag taaaggtatt tttaacaaat acaatatcaa
    35701 tatcaacaat gtgaacaaga cccttagtag aaatcttagt tgataaacta gcaccatata
    35761 aggaagctaa cttttctcga aattcttgta ccttagggta cttagcattt gcagtaacta
    35821 acatttgagc aacgagcgat ctacgtgcta ctgttttatt attaaaatca cctgaaaatc
    35881 gaaaagtaag atgattagtc ttaaattgtt ggtttttaat taaatgtaga gaaacgcctt
    35941 caactatttt cattcaatat cctttgtgtt ctgtattgtt agctaagatt atatcatttt
    36001 taaaggcgtt aaaaaagatt aaacatccaa gatatgatta attctaaatt tcaatcttct
    36061 tcaaaattga caaagtcgtc aaccaatatt tactcccatt ttttatacct tgtctccata
    36121 tcataagttc aactcaaaaa ataattttat gctataatga tgtgatatct aaaattggag
    36181 attaaaaagt catggattat aaattatttg atgaatatat tactttacaa tcacttctaa
    36241 aagagattgg tatcattcaa agtggcggag ccattaaaaa atttttagcg gataatcaag
    36301 tattgtttaa cggtgacctt gaaaataggc gtggaaagaa actccgcctg ggggatatta
    36361 ttactattcc tgatcaaaat gtcgagatca ttataaggaa gccatcagat caagaaattg
    36421 aagaacgtaa tgtagaaata gctgaaaaac agcgtgtttc agcaatcgtt aaagaaatga
    36481 ataaaaacac caacaaagat aaatcaaaaa ctagcaaaaa accagttcgc tttccaggga
    36541 cataaaaatg tggataaaaa atatttcttt aaaacattat cgtaattatg aagaagcaca
    36601 agtagatttt tcacccaacc tcaatatttt cattggccgc aatgctcaag gaaagacaaa
    36661 ttttttagaa gcaatctact tcttggcttt gactcgaagt catcgtaccc gttctgataa
    36721 agaacttgta cattttaaac accatgatgt acaaattaca ggtgaggtaa tccgcaaaag
    36781 cggccatctt agcttagata ttcaattatc tgaaaaagga agaatcacta aggttaacca
    36841 tcttaaacag gcaaaattat ctgattatat tggggctatg actgtcgtac tttttgcccc
    36901 tgaagatctt caattagtta aaggtgcacc tagcctaaga cggaaatttt tagatataga
    36961 tattggacaa attaagccaa cgtacttagc tgaactttca aattacaatc atgtattaaa
    37021 acaaagaaac acttatctaa aaactactaa taacgtcgac aaaacttttt tatcagtcct
    37081 tgatgaacaa ttagctgatt atggaagtcg tgtcattgaa caccgttttg attttatcca
    37141 agccctaaat gatgaagcgg acaagcatca ctacattatc agtactgagt tagaacattt
    37201 atctattcac tacaaatcgt caatagagtt tacagataag tcaagtatta gggaacattt
    37261 tttaaaccaa ttatctaaaa gccactcaag agacatcttt aagaaaaaca caagcatcgg
    37321 acctcatcgt gatgatatta ctttctttat taatgatatc aatgctactt ttggtagcca
    37381 agggcaacaa agaagcctta ttttatcatt gaaattagca gaaattgagc taataaaaac
    37441 cgttaccaac gattatccga ttctcttact tgacgacgtc atgagtgaac ttgacaacca
    37501 tcgtcaacta aaactcttag agggaatcaa agagaatgtt caaacattta ttactacaac
    37561 aagtttagag catttaagtg ctttacctga tcaactcaaa atctttaatg ttagtgatgg
    37621 tactattagt attaatgaaa aaaaagcaac agattaatct gttgcttttt aataagattt
    37681 aacaatacca agtaatatag cacccataac aaaacaaagg atacccatga caacccattt
    37741 ctgctctttc ttcgtttttg tctcacctaa aaataaaata ccaccaataa tagagataat
    37801 tactccaagt tgagaaaaac taaaagcaat tgctagccct gctttagctg ctgccaataa
    37861 catgaagaca ttaccaacgc cccacatgag acctgtaatc atatttttaa caacaacagc
    37921 ctcaaagtta acacgaaact tcatgaaaca aattgcacct agacacattc caacagccat
    37981 gggtaaaata acggccatag cgtcgaactt cataatgtta ttaaataaaa ctgcgtatga
    38041 gatgtaacct acagttgaat aagcaattgt agcaaatcct tttgaaaact cttgatgtgt
    38101 tgccaaagct tgttctgaga catcacgttt acttgagaaa tagaagccga taactaataa
    38161 tgtcaacgct gtcaatccta aaataaattg gattggctta gtccattcat gaaagactaa
    38221 agcaccaact aggcttccac ctactaattg tgcaccactt gacagtggat tagcaacaga
    38281 gactcccata tatttcattg cttgaaattg accattttgg ccgactgacc ataggatacc
    38341 acctaagata ccaaaaatcc acaatgaggc agtcatctct ggctgtttaa ataaccatac
    38401 gataatcgca aatagcaatg ctcctaaagt cattccaaat gtttgttgat ttggacggcc
    38461 tccaatttta ttactaacaa atccaatact tccccacgca aacatgggaa tcaatgcaat
    38521 aagtaaacct tccaattctt tctccttaca atggaatatt aagtattacc tattttcaat
    38581 cctagagaat ctattgacta actttattag caaaatcagc aaatactata gctttatcaa
    38641 cactatcttt ttccaaagct ataatgactt gttttatcaa taaattgacc agtgatttag
    38701 aataaggtaa ctgttcttta gtcatattac taatcttatt taaaataaca tctttaggta
    38761 aaatatcttg aaaatagcag atgactttga aataaaaatc attgtactgt gtcacaccat
    38821 tcagataagt aactaccagg ttaatctcat cttgaggcag cctcatattt aaccctaaat
    38881 ttgaaataaa aagtttagcc aatattttga gaacattgct ttctattcta tcacaggatt
    38941 gttgtttttg gaaactgaaa attctcatga tatcttcatt atctcgataa gattccagta
    39001 aagttaattg ctctaaatca gtattagaag ttccagcctt aacttttgaa tctagaatgg
    39061 atacttcatt gatagttacc ctaacctcca acgtttgtaa agctttaata aatttatcaa
    39121 tacctatttg tgtctcacca ttttcaaaac gtgatagagt tgattttgat aattgagcac
    39181 ttttggctag agaacttagg gatatacctt tgtcttctct caactctttt aaaatttttc
    39241 cataattttt catacaaaaa ctttactata tttgtttctt ttatgcaaca aaaagcatcc
    39301 tcatagggga tgctttttgt ccttatttta gtgtactgaa taatttggtg cctcatttgt
    39361 aatttgaaca tcatggggat ggctttcttt caaaccagct cctgacattt caacaaattg
    39421 agcattatca tgaagttctt ttatgttcgc tgcacctaca tatcccatac cagaacgaat
    39481 gccaccaagc atttggaaaa caatatcggc tactgaacct ttgtatgcta cacgaccttc
    39541 aataccttct ggaaccaatt tgttagcctc gtttacagaa ccttggaaat aacgatcgct
    39601 tgaacctttt ttcattgctg caattgagcc cataccacgg tatgtcttga acttacgccc
    39661 ttggaaaatt tctgtttctc ctggcgcttc atctgttcct gcaaacattg aacctagcat
    39721 aactgcattt ccacctgctg caagtgcttt tacaatatca cctgaatatt taataccacc
    39781 atcagcaatg atagtcttac cgtactcacg cgcaacagcc gctgcatcgt aaatagctgt
    39841 aatctgagga acacctaccc cagcaacgac acgagtcgta caaattgaac ctggaccaat
    39901 accaacttta acgacatcca cacctgcatc ataaagtgca cgcgctcctt ctgctgtagc
    39961 aatatttccc gcaatcaatg tgcgattagg gaagtgagca cgaatttcag caattttacg
    40021 gagcacacca gctgaatgtc catgtgctgt atcaatgaca atagcatctg cacctgcttc
    40081 aaacaaagct tctgcacgtt caaaagtatc agaggttaca cctactgcac cagcaactaa
    40141 gagacgtcca aattcatctt ttgctgcctt cggaaattca ataacttttt cgatatcctt
    40201 aatagtaata agtcctgaaa gacgtccctc atcatctact aaaggtagct tttcaatacg
    40261 atgctcatga agaatacgct ctgcagtttc aagatctgta ccaataggag ctgttacaag
    40321 attttgactt gtcatgtgct ctgagattag ttgtttataa tcagaaatga aacgcatatc
    40381 acggttagta ataattccaa ctaatttacg attctcgagt gtctctacaa taggaactcc
    40441 actaatgcga tagttttgca tcaattcctc agcttcagag acagtattat ctggtgtcaa
    40501 gaagaatggg tcaataataa caccattttc tgagcgttta actttacgaa cttcttctgc
    40561 ttgatcaact attgacatat ttttatgaat gataccaagc ccaccagcac gcgcaatagc
    40621 aatagccatc ttactatctg taactgtatc cattgcagct gtaatgattg gaatgtttaa
    40681 tgtcaaatta tcagccaact ttgtcttcat atcaacttca tttggtaaaa catggctttc
    40741 agcaggaata agcagaacat catcaaaagt aaaacctttt ttcaaaaact tagtatccca
    40801 atttgacatc aaaaaatcct cttttcttag atagtagata taaaaaatcc cattaaaatt
    40861 atattgttac tatcatatca cgctaaaatc atttgtcaat ataaaaagag catagaatta
    40921 atagcttcta gctctttaaa gtaatgaaaa aatcttctta attttctatt tttttgagaa
    40981 gaagaaaggc ggcgtatact gacttaattt ctcaaaacac ttttgagctt cttcactatt
    41041 attacaaata atcaaacaag tatcatcacc acagacagtt gctaagatct caggtaataa
    41101 aatcgaatct aatatggagc caaaagattg agctaagcca ggtagtgtct tcaaaacgat
    41161 ttggttttga actggaaaca gcatgactag ggcatcttcc atgtaaaaac gtaaccgttc
    41221 ttcccaacgc ttctgagata tactatgaat ctcataataa gtttctgtag cattatcact
    41281 gctttcgtta accttaatta aattaagttc cttcatatct cgagataatg ttgcttgcgt
    41341 cactgaaacg ccattttttt ctagtaattc ccttaactca tgttgtgtac gcaccttagt
    41401 ctcagagact aaggaacgaa ttaattggtg tcttgtttct tttttattca tataaactcc
    41461 ttattcaatg aatttcttga aataattttt attattatag agaagcttct ttcctttcat
    41521 ttcaacgata tctttttggc gtaattcctt taaaacatga ctaactgtct cgcgagtagt
    41581 tccgctcata ttggcaattt caatcgttgt aataggaaaa ggtaaatgtc ccctttctgt
    41641 aggaacttgc aaaagtagag ttgcaagcga ttggataact ctcatactgg cacttgaagt
    41701 caccatatta cgaagtcgta tttcatgaac tcgtaatagc ttagaatatt tccgacaaag
    41761 atgcttcatt tgattgatat tttgaagaga gtattcttcg tataaagcca ttggcagaga
    41821 aaaatactca atatccgtaa tagccactgc tgaaaaatgg taatcatcat ccaaaaagag
    41881 acctccataa ggaaaaaccg tatcttgtct aacataatct gtataaataa aagaaccaga
    41941 agcgtctgat tgttcaattt ttacatgtcc tgactggatt aaaaacagtt tttcacggta
    42001 atcaccttca aaaaataagg tatgattttt tagagctgta cgatgtttaa tgtggctcac
    42061 aatatggtca aattgttcaa tagtaaagtg tttaaaatca tctagttgtc tgaaataaaa
    42121 atattgttct tttgtaatca cctgattttc tagacctttc taatcattag atgcgaacat
    42181 tttttgtata atgaaaattt tttaatgtat aaaattttct tgtcacgata aatcctcctt
    42241 cattatagca aaaaaattcg tagaaagaat gtttttcgtt gaattttgaa aacgcttttg
    42301 agaaaatgta attgttctga tatcttttaa aaaaattgag aggccttcat gactaaattt
    42361 attgttgatt catcgtactg gaatttgttt ccaacttcta aaattggagt aatcttaatc
    42421 aaagattatc acatggacag gaaccttgaa acagaactta aacagttact gtctgatagt
    42481 cattctttag caaaaaaata tttgcaagag aaagaattca gtcaaaacag agttattcaa
    42541 acttatcgta aagcatatca aacctttaaa actaaaaaag gtgcacgttc aagtatcgaa
    42601 gcattattga agcgagttaa ttcaggaaac gaaattacta gtataaatcc tctagttgat
    42661 atctataatg ccgctagcct taggtttgga ttacctattg gtgctgagga tagtgataca
    42721 tttagaggtg atttaaaact aactattacc aatggtggtg atgaatttta cttgattggc
    42781 gaagatttta acagaccaac tctttcaggt gagcttgcct atgttgatga tgttggagca
    42841 gtttgccgct gttttaattg gcgtgatgga aaacgtacta tgattaccga taatactcag
    42901 aacgcttttt tagttatcga attaattgat aatggaagag aaattatttt taaagaagcg
    42961 ttggacttta tcgccaccaa tacaaatcgt tttctaaaag ctaaaacaca gaccatcata
    43021 ttagataaag aacacagtga aataacgcta taacattaaa acgaaaagta aaaattttac
    43081 ttttcgttat ttttttaggt taataatttc tcactttcca acgataaaag aaagaaattg
    43141 catagaaatc gctttcattt ctataaattt aaattttttt ggatataatc gtgacattct
    43201 taacactttt aatgcgaata acttccctat tttatattca aaagaaagta gaatgtaaat
    43261 gtagatatag agataaatcc tgtttcaata caaatctcta actaagaaag gagtaaaata
    43321 tgactcaaac acatccaatt catgttttct cagaaattgg aaaacttaaa aaagttatgc
    43381 tacaccgtcc aggtaaagaa atagaaaatc tgatgcctga ttatttagag agactcttgt
    43441 ttgatgatat cccatttttg gaagatgcac aaaaagaaca cgatgcgttt gcacaagcac
    43501 ttcgcaacga aggagtcgaa gtactttacc ttgaaaatct tgctgccgag tcgttaacaa
    43561 accaaggaat tcgtgaacag tttatcgatg agtacattgg cgaagcaaat gttcgcggtc
    43621 gtgctactaa aaaagctatt cgagaattat tactcaatat caaagataat aaagaactta
    43681 tcgaaaaaac tatggcaggt atccaaaagt ctgagctacc tgaaatccca tcttctgaaa
    43741 aaggtctaac tgatcttgtc gaatctaatt atccatttgc catagatccg atgccaaatc
    43801 tttactttac acgtgatcca tttgcaacta ttgggaatgg agtttcatta aatcatatgt
    43861 tctctgaaac acgtaatcgc gaaactttat acggtaaata tatctttaca catcacccag
    43921 agtacggtgg aaaagtccct atggtctatg aacgtgaaga aacaactcgt attgaaggtg
    43981 gagatgagct tgtcctttct aaagatgtcc ttgccgttgg tatctctcaa cgtactgatg
    44041 ctgcctctat tgaaaaactc ttagtcaata tttttaaaca aaatttaggt tttaaaaagg
    44101 ttttagcatt tgaatttgca aataatcgta aatttatgca ccttgacaca gtctttacaa
    44161 tggttgacta tgacaagttt acaattcacc cagaaattga aggcgatctt cgcgtttact
    44221 ctgtaactta cgaaaaccaa gatcttcata ttgaagaaga aaaaggtgat ttggcagatc
    44281 ttctagcgaa aaacctagga gttgagaaag tggaattgat tcgttgtggg ggtgacaact
    44341 tagtcgctgc tggacgtgaa caatggaatg atggttcaaa tacattaact atagcaccag
    44401 gtgtcgttgt cgtttacaac agaaatacaa tcacaaatgc aatcttagag tccaaaggat
    44461 tgaaattaat taagattaat ggaagtgaat tagttcgtgg gcgtggtgga ccacgctgca
    44521 tgtcaatgcc atttgaacgt gaagaccttt aaaatttatg aagctattag tcaatcactt
    44581 gccttaccaa agagctgcaa gcctatatat tcgcttttct gttttcgtta tcgaaagaaa
    44641 tataaaaatg gaagaagaat tcgatgataa tgacgagcaa gatactattt atgctgttct
    44701 ttatgatggg aaacagccag tttcaacagg acgctttctt cctgaaactc aaacagaagc
    44761 tcgtttgact cgaattgcga ctttaaaagg ttatcgtggt aacggctatg gtaccaaaat
    44821 tattatagca ctagaaaatt atgcaaaaga aaatggttat cactatctaa ctatacatgc
    44881 tgaattgaca gcaaaagatt tctaccagac acttgggtat caagctactg gggatattta
    44941 tatggaagac ggggaagcct gtcaaacact cgaaaaatat ttaatttaaa ggagacatta
    45001 acatatgaca caagtatttc aaggacgtag tttcttagca gaaaaagatt tttctcgtga
    45061 ggaatttgaa tatcttattg atttttcagc tcatttaaaa gaccttaaaa aacgtggtgt
    45121 tcctcatcat tatcttgaag gtaaaaatat tgctctctta tttgaaaaaa catctactcg
    45181 tactcgcgca gcctttacaa ctgcagcaat tgacctaggc gctcatccgg aataccttgg
    45241 tgcaaatgat attcaacttg gtaaaaaaga atcaacagaa gatactgcta aggttttagg
    45301 acgtatgttt gatggtattg aattccgtgg ttttagccaa agaatggttg aagagcttgc
    45361 tgaattttct ggagtacctg tctggaatgg tttaacagat gaatggcatc caacacaaat
    45421 gctagctgac taccttacta tcaaagaaaa cttcggtaaa cttgaaggta ttactcttgt
    45481 ttactgtggt gacggacgta acaacgttgc caactcactt ttagtggctg ggactttgat
    45541 gggagtcaat gtacacatct tttctccaaa agaacttttc cctgctgaag agattgttaa
    45601 attggctgaa ggatatgcca aagagtctgg ggctcacgtt ctcgttactg ataatgtaga
    45661 cgaagctgta aagggagcag acgtctttta cactgatgtc tgggtatcga tgggagaaga
    45721 agataagttc aaagaacgcg ttgaacttct tcaaccatat caagtaaaca tggatctgat
    45781 taaaaaagct aataatgata atcttatctt cttacactgc ttacctgcgt tccatgatac
    45841 aaataccgtt tatggcaaag acgtcgctga aaaatttgga gtcaaggaaa tggaagttac
    45901 tgatgaagtc ttccgtagca aatatgcccg tcatttcgac caagctgaaa atcgtatgca
    45961 cactattaaa gctgtaatgg ctgcaaccct tggaaatctt ttcattccaa aagtttaaat
    46021 taaacgacta tagggagttg acatatagtc aattccctat tttaggaaat gaggtataaa
    46081 atggaaaaag aaaaaaaatt aggtctttta ccactaacaa tgcttgtcat tggctctctt
    46141 atcggtggcg gaatctttga tttaatgcaa aatatgagtt ccagagccgg tttggtacca
    46201 atgcttattg cttgggtaat tactgctatc gggatgggga ctttcgtttt aagttttcaa
    46261 aatttatctg aaaaaaggcc ggacctaaca gctggaatct ttagttacgc taaagagggg
    46321 tttggaaact ttatgggatt taactctgca tggggttatt ggttatcagc ttggcttgga
    46381 aatgttgcct acgctgcact cttattcagt tcactcggtt atttctttaa attctttggt
    46441 aatggaaata atatcatttc aattattgga gcaagtatag ttatttgggt tgtccatttc
    46501 ttaattttaa gaggtgttaa tacagctgca tttattaata ccatagttac ctttgcaaaa
    46561 ttagtacctg ttattatttt cttaatttca gcgttattag ctttcaaatt taacattttt
    46621 agtcttgata tctggggaaa tggattacat caatcaattt tcaaccaagt caattcaact
    46681 atgaaaaccg ctgtttgggt atttattggt attgagggcg ccgttgtctt ctcaggtcgt
    46741 gctaaaaaac actctgatat tggtaaagca agtatcctag cattattcac tatgatttca
    46801 ctttatgtat tgatttctgt tttatcactt ggtatcatgt cacgtccaga acttgcaaac
    46861 ttaaaaacac cagctatggc ttacgttcta gaaaaggctg ttggtcactg gggcgctatc
    46921 ttagttaacc ttggtgttat catttcagta tttggcgcta ttcttgcttg gactttattt
    46981 gcagcagaat taccatatca agctgctaaa gaaggtgctt ttcctaaatt ctttgcaaaa
    47041 gaaaataaaa acaaagctcc aatcaactca ctcttagtca ctaatctttg tgtacaagca
    47101 ttcttaatca cgttcttatt cacacaaagt gcttatcgtt ttggtttcgc attagcatca
    47161 tctgctatct taattcctta tgcttttaca gcactatatc aattacaatt cacactccgt
    47221 gaggataagt caactccagg acatcaaaag aatttaatta tcggtatcct cgctacaatc
    47281 tatgctgttt accttatcta cgctggtggt tttgattact tacttttgac aatgattgct
    47341 tacactctag gtatgattct ctatattaaa atgagaaaag atgacaagct tcctatcttt
    47401 gtgggatatg aaaaaattag tgccattgtc attctagcgc tttgcttact atgtattatt
    47461 gaaattatga ctggacaaat tgacattgct gcaatcctag caggttagtc attagaaaga
    47521 gggataaatt ggtaaaagaa catcaaaaaa tcgtagttgc cttaggtggt aacgctattc
    47581 tatcaacaga tgcttcagca aaagcacaac aagaagcatt aatcaacaca tcaaaatcac
    47641 tggtcaaact gattaaagaa ggccatgatg tcattgttac tcatggaaat ggtccccaag
    47701 ttggtaactt attgctacag caagcagctg cagattctga aaaaaatcca gcaatgcctt
    47761 tggatacttg tgttgccatg acagaaggaa gtattggttt ttggttacaa aatgctctta
    47821 acaatgaatt acaagaacaa ggcattgaca aagaggttgc aacagtggta actcaagtta
    47881 ttgttgatga aaaagatcaa gctttcacta atccaacaaa acctattggc ccattcttat
    47941 ctgaagaaga tgccaagaaa caagcccaag aaacaggttc aaaatttaaa gaagatgctg
    48001 gtcgtggatg gcgtaaagtc gttccttcac caaaaccggt tggtatcaaa gaagcttctg
    48061 ttattagacg gttagtggac tctggtgtgg ttgttattag cgcaggtggc ggaggtgtac
    48121 cagttattga agatgctaat acaaaggcat taaaaggggt tgaagctgtt attgacaaag
    48181 actttgccag tcaaacttta tcagaacttg ttgatgctga cttattcatc gtcttaactg
    48241 gcgttgacaa cgtttttgtt aattttaata aacctaatca agaaaaatta gaagaagtta
    48301 ctgtttcaca aatgaaacaa tatatcactg aaaatcaatt tgcccctggc agtatgctac
    48361 caaaggtaga agctgcgatt gcttttgttg aaaataaacc agaagcgcgt gctatcatta
    48421 cttcacttga aaatattgat aatgtcttag cagaaaatgc agggacacaa attgttgcaa
    48481 attaaacaat gtagattacc attacataca ctccttatta ataacaaagt aatgtcactc
    48541 cttagg
//
LOCUS       DATRNK010000008        44597 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.008, whole genome shotgun sequence.
ACCESSION   DATRNK010000008 DATRNK010000000
VERSION     DATRNK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44597
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.008"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            8..268
                     /locus_tag="VBC52_000464"
     CDS             8..268
                     /locus_tag="VBC52_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6882070.1"
                     /translation="MQVILPDEQVKEIQLLVSNVIKSEIQQFKTDIGLEKPYLNKLQT
                     CQYLGISNNTLDSWIKLGLPVIKIGKTIRFDKNAINSWLQSH"
     gene            329..1549
                     /locus_tag="VBC52_000465"
     CDS             329..1549
                     /locus_tag="VBC52_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO6882071.1"
                     /translation="MTITKTKNGTYRLRVYIPEEAKKSLGIDKKLIEKRFKLRSDAKK
                     YELELQNKIDNVLNGSFQNIHDTGDILFSEFYKNIWWNSYKSGQTTTTNKPPSKVTID
                     NTEIVFRLHILPLLGNYSINFLNQNKQVVLNLLTKKAEEYANFKVIRSYVNSIFDWAE
                     ELEYIEANRVSKTIKRIKATKKIMLQESKEDSDLFLSQKELLEWFKAFEEDLLNNKIL
                     FKDYVLFYTTFFLGDRKSETYALQWKHIDFNSRQIQLVQALDKYKNKKATKGNKKTTF
                     NIPLELQELLISWKNQQKLELSKLGISQTNDQYVFTYIDTKGNLNSPLHTDYLNNKMK
                     SVNRRHPELKHATPHKLRHTGATLAKQAGTSLEDISQALTHSDTLITKTYVNTSNIVP
                     MTVGEIAFRNLKNE"
     gene            1952..2239
                     /locus_tag="VBC52_000466"
     CDS             1952..2239
                     /locus_tag="VBC52_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882072.1"
                     /translation="MVTAEKNRAVTFQANKELVSEAMTVLNKKNLTLSSALRLFLQNV
                     VVTNEVDLLTEEELEKEKLFKQFQAEINKNIEDVRQGKFYTSEEVRSELGL"
     gene            2229..2564
                     /locus_tag="VBC52_000467"
     CDS             2229..2564
                     /locus_tag="VBC52_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEO6882073.1"
                     /translation="MDYKKYQIIYAPDVLEKLKEIRDYISQNYSSTSGQHKMEQIISD
                     IEKLEVFPEVGFDADEKYGSKISKYHSTRGYTLSKDYIVLYHIEEEENRVVIDYLLPT
                     RSDYMKLFK"
     gene            complement(2869..3339)
                     /locus_tag="VBC52_000468"
     CDS             complement(2869..3339)
                     /locus_tag="VBC52_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882074.1"
                     /translation="MTDKKIVYYFDHDLSKKDMGGVGPTYTGKNLLFLLWWTYSYVAE
                     DNLDIDTSLFEVPEFNDFYNNGLELYRIKNELANYNIRFGYEVNPKDSTKMLIGILPT
                     DEEDYVKGYRNFIADISNPDTRYAIYHRLIKTMDTMLPIEQINVYKSLAKGNKK"
     gene            complement(3507..4763)
                     /locus_tag="VBC52_000469"
     CDS             complement(3507..4763)
                     /locus_tag="VBC52_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEO6882075.1"
                     /translation="MSYVVARMAKYKSGQLTAIYNHNERIFKNHSNKEIDVEKSHLNY
                     ELTNRDQAQNYHKQIKEHINENRLSTRGVRKDAILCNEWIITSDKTFFDSLDEKQTRE
                     FFETAKDYFAEKYGDANIAYARVHLDESTPHMHLGIVPMKNGKLSSKALFGNKEKLVA
                     IQDELPKYLNEHGFNLQRGEIGSKKKHLETAEFKEKQRLLDNADRKLADKHEELKALD
                     DKISNVNDTIADKESRLKELEAKEWDAVGDLKQYELEKQSLAESIEDIKDIELLQLDR
                     IQKEDLVKQSFDGKLKMDKETYNRLFQTASKHASSNAELKRDLVKAQSQNNHLSRELL
                     NHRKTADKNIKLSQENRKLKDKVKMLDEQVKILNKNLSVWKEKAKEFMPKQVYRETLS
                     IINSLNPIGLAKTAIRQVKKMVDSNS"
     gene            complement(5079..5513)
                     /locus_tag="VBC52_000470"
     CDS             complement(5079..5513)
                     /locus_tag="VBC52_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882076.1"
                     /translation="MRIKDYADSLGVSSQSIYKRIRSPKYKERLKGHLYRDNQKVENL
                     DLIGIKILEDYHFENDVIELEKTLGDIQEEFEQEKKGMQYRIDRLADKLTPLLEDNQN
                     LVQKNYELLNYVRSLERQKLLLIIALAVMVITLLVAIWLAIF"
     gene            complement(5549..6424)
                     /locus_tag="VBC52_000471"
     CDS             complement(5549..6424)
                     /locus_tag="VBC52_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEO6882077.1"
                     /translation="MKLKVSLDNITMTAYIKSKKYLAMKQLIETHLAITVQTAMTDMF
                     RATTGDGIHVVLHMNYDKQKGQDRKARPFRLEFNPNKLRLVDSEIIDTIIPFLEDISI
                     SRADLAFDLFEVDCSEFVLEKKGRPTATKEFRSSTGTLETKYLGAPRSEKQVRLYNKK
                     KEQLQNGTDKDKDFASQFKHWWRLEFQLRSRSIDEIFEVIDTIIFKPFNLKGLSIETQ
                     IYLTALIHDKNIWKKLHRNTRARYKKILETHQTSDTDYLGLLKDLLKHERPRLENQLA
                     YYGGRIDKNSFQPFC"
     gene            complement(6737..7366)
                     /locus_tag="VBC52_000472"
     CDS             complement(6737..7366)
                     /locus_tag="VBC52_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882078.1"
                     /translation="MIKIYFGKDITLNQAIQSRLDSYQIDYQAFSSKDIDAKTLMEWL
                     FRSTDIFELLSTKMLKYKLNTQITLSQFVRKILKDVNSTLKLPIVVTDEVIYSNMSLD
                     YVTVLLPKEYRKIERIQLMRKMEQLDEGRLFWKNFESLRKQSELRWFELNELLFADMS
                     DDLGEIKKAKDRFFSYKKNKQVPPDDIIEKILKIFLVDREDFFKKSVLD"
     gene            complement(7370..7696)
                     /locus_tag="VBC52_000473"
     CDS             complement(7370..7696)
                     /locus_tag="VBC52_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEO6882079.1"
                     /translation="MDYDNDSYLIPKVLLQDDFYSSLSARDILVYAVLKDRQIEAPEK
                     GWIDTDGSIYLNFKLIELAKMFSCSRTTMIDIMQRLEEVNLIERERVDVFYGYSLPYK
                     TYINEV"
     gene            complement(7709..7939)
                     /locus_tag="VBC52_000474"
     CDS             complement(7709..7939)
                     /locus_tag="VBC52_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882080.1"
                     /translation="MDKNNLEDLTGKKILRNGFGGLRLEYGTIFLDVGLFTYIVLILS
                     IFFFLVERWFLGALTFVLHVPLLLLGIWCGER"
     gene            complement(7950..8228)
                     /locus_tag="VBC52_000475"
     CDS             complement(7950..8228)
                     /locus_tag="VBC52_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO6882081.1"
                     /translation="MTEGFTIQLPKVTEKKLLARYDTMVQKAIEKALEDKELYKPMVR
                     MAGLCRFLDVSTTTIVKWQKAGMPHMVIDGVTLYDKRKVAQWLQQFER"
     gene            complement(8364..8669)
                     /locus_tag="VBC52_000476"
     CDS             complement(8364..8669)
                     /locus_tag="VBC52_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882082.1"
                     /translation="MAYTLEERETIVRYDEMDNCWYFESNVRKHITKIMKTIDSCQIL
                     GQEKEDDRIIWIHAKLTNLDDYMVSPFVRKRVKREMTEEQREEARKRMARLHSKSEI"
     gene            8824..9414
                     /locus_tag="VBC52_000477"
     CDS             8824..9414
                     /locus_tag="VBC52_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6882083.1"
                     /translation="MTNFQLLVKESNKSLTEISQETGISYSTLGNYNQGTRTPKKENA
                     KILADYFDVSVPYLLGLDDNRQILDFAGEGIAKGIIEKIKGRKTLDMLLTSTPYLEEN
                     VDIMNDIINKKVLDSYIDFLYEGKYPPAVIVFIKDVIKNRFDDIPALANRSSDKNSEY
                     HFLWEAWEKEPRKKERTEQTRKKWKEQGIPIFKSWK"
     gene            9478..10632
                     /locus_tag="VBC52_000478"
     CDS             9478..10632
                     /locus_tag="VBC52_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6882084.1"
                     /translation="MSIHKYPSKKAKNGYLYLVKVYMTRDAQRADHIKRGFRTRKEAK
                     DYEARLIYLKASGRLEEFIKPTHKTYNEIFEKWYQAYQDTVEPTTASRTLDMFRLHIL
                     PVMGDLPISKISPLDCQNFITEKAKTFKNIKQIKSYTGKIFDFAIKMKLLKHNPMAEI
                     IMPKRKKTQIENYWTVQELKEFLAIVLQEEPYKHYALFRLLAYSGLRKGELYALKWAD
                     IDFQSETLSVNKSLGRLDGQAIEKGTKNDFSVRKIKLDSETISILQEWKANCQKEKAQ
                     LAVAPLSIKQDFIFTYCTRSGSVEPLHADYINNVLSRIIKKHGLKKISPHGFRHTHAT
                     LMIEIGVDPVNTAKRLGHASSQMTLDTYSHSTANGEDRSIKQFADYLKAK"
     gene            complement(10760..11152)
                     /gene="rpsI"
                     /locus_tag="VBC52_000479"
     CDS             complement(10760..11152)
                     /gene="rpsI"
                     /locus_tag="VBC52_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEO6882085.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITINKKDVEEYIPHADL
                     RLVINQPFAVTSTQGSYDVFVNVVGGGYAGQSGAIRHGISRALLEVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(11173..11619)
                     /gene="rplM"
                     /locus_tag="VBC52_000480"
     CDS             complement(11173..11619)
                     /gene="rplM"
                     /locus_tag="VBC52_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEO6882086.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDAADVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKASDKIYYTHSMYPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            complement(11841..11963)
                     /locus_tag="VBC52_000481"
                     /pseudo
     CDS             complement(11841..11963)
                     /locus_tag="VBC52_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002278327.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(12500..13360)
                     /locus_tag="VBC52_000482"
     CDS             complement(12500..13360)
                     /locus_tag="VBC52_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6882087.1"
                     /translation="MTFKILTDSTSDLDEKWAQEHNVDIIGLTIELDGKTYETVGDEK
                     ITSDFLLERMQEGAKPTTSQINVGQFEEVFSTYAENDHALLYLALSSHLSGTYQSATI
                     AREMVLDKYPDAQIEIVDTMAASCGEGVLAMLATKERQEGKSLEEVKQKIESLLPKLN
                     TYFLVDDLNHLMRSGRLSKGAAIIGSVAKIKPLLKLDSEGKLVPFAKTRGRKKGIKEI
                     VTQATKTLSYSTLIIAYSGEKDSAQVMKEQLLADERIEEVIIRPLGPVISAHVGSGAL
                     ALFSLGEENR"
     gene            complement(13453..13971)
                     /locus_tag="VBC52_000483"
     CDS             complement(13453..13971)
                     /locus_tag="VBC52_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEO6882088.1"
                     /translation="MKKHSILLVDGYNMIAFWKDTRQLFKSNRLEEAREVLLRKLNHY
                     AHFEHIDIICVFDAQYVPGVRQRYDQYKISVIFTEEDETADSYIERAAAELNQSVLNL
                     VSVATSDLNEQWTIFSQGALRVSARELEQRVATVKSDLDKMSSQIDLSTPKLRPWNDE
                     QLGKLKDFLDGM"
     gene            complement(13968..14723)
                     /gene="rlmB"
                     /locus_tag="VBC52_000484"
     CDS             complement(13968..14723)
                     /gene="rlmB"
                     /locus_tag="VBC52_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEO6882089.1"
                     /translation="MTMKDKQFKEESSDLVYGLHAVTESLRANTGNKLYLQDDLRGKN
                     VDKVKALATEKKVSISWTPKKTLSDMTNGGVHQGFVLKVSEFAYADLSEIMTKAENEE
                     NPLILILDGLTDPHNLGSILRTADATNVTGIIIPKHRSVGVTPVVSKTSTGAVEHVPI
                     ARVTNLSQTLDTLKDKEFWIFGTDMNGTPSHKWNTKGKLALVIGNEGKGISHNIKKQV
                     DEMITIPMNGHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(14826..15212)
                     /locus_tag="VBC52_000485"
     CDS             complement(14826..15212)
                     /locus_tag="VBC52_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEO6882090.1"
                     /translation="MIDVRLINGIALAFEGDAVYSLYIRRHLIMQGFTKPNQLHRKAT
                     QYVSANAQALLINAMLEENILTDEEQLIYKRGRNANSHTKAKNADIITYRMSTGFEAL
                     MGYLDMTGQIKRLETLIQWCIETIEK"
     gene            complement(15205..16548)
                     /locus_tag="VBC52_000486"
     CDS             complement(15205..16548)
                     /locus_tag="VBC52_000486"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEO6882091.1"
                     /translation="MIKIYDTMTRSLQDFIPLNEGKVNMYVCGPTVYNYIHIGNARSV
                     VAFDTIRRYFEYCGYQVNYISNFTDVDDKIIKGAAEAGMDTKSFSDKFISAFMEDVAA
                     LGVKPATKNPRVIDYMDEIIDFVKVLVDKEFAYEANGDVYFRVSKSHHYAKLANKTLE
                     DLEIGASGRVDGEGEIKENPLDFALWKSAKSGEVSWESPWGKGRPGWHIECSVMATEI
                     FGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVNVDNEKMSKSLGNF
                     ITVHDMLKSVDGQVIRFFLATQQYRKPVNFTEKAVHDAEVNLKYLKNTFNLPIQENAN
                     DEELEQFVKAFQGAMDDDFNTANGITVIFEMAKWINSGHYTSRVKETFAELLEIFGIV
                     FQEEVLDADIESLIEQRQEARANRDFATADRIRDELAKQGIKLLDTKDGVRWTRD"
     gene            complement(16545..16727)
                     /locus_tag="VBC52_000487"
     CDS             complement(16545..16727)
                     /locus_tag="VBC52_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEO6882092.1"
                     /translation="MKDYVTTQILSILGILAAACLSYLKFAQGNPLLGIIWAAFVLMY
                     VVRLYKAYQKQKKEKP"
     gene            complement(16737..17321)
                     /gene="cysE"
                     /locus_tag="VBC52_000488"
     CDS             complement(16737..17321)
                     /gene="cysE"
                     /locus_tag="VBC52_000488"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEO6882093.1"
                     /translation="MGWWKESIAIVKEQDPAARSSLEVILTYPGIKALAAHRLSHFLW
                     NHNFKLLARMHSQFWRFWTQIEIHPGATISEGVFIDHGSGLVIGETAIVEKGAMLYHG
                     VTLGGTGKDKGKRHPTIRKGALISAHSQIIGPIEVGENAKVGAAAVVLADVPADVTVV
                     GVPAKVVRVHGQKDDLQIRSIEHDREESYYSSKL"
     gene            complement(17330..18082)
                     /locus_tag="VBC52_000489"
     CDS             complement(17330..18082)
                     /locus_tag="VBC52_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEO6882094.1"
                     /translation="MTSTNELDIRLRAFINAPDNFLDSIGLVNALHHSTVWASKEPYA
                     IQVDGQEVVPVFTDITDLNHFKEEQESARDMFWESRRSLDVLDEAISHGLAGLVYNLK
                     KEGDFGNSTIFYCEDMVQFMNNYTTILNQLLNEDNIVADIMDKTYLVPAFVHPREEGS
                     FDRLFPTMSTPEGKSYVPVFSNLLSFEKWYNHNDFGGAFRKAQGVILAWTIDDIYKPR
                     NGENEIDDTFGVAINPFDEQQVLVDWSDVEND"
     gene            complement(18084..20213)
                     /gene="pnp"
                     /locus_tag="VBC52_000490"
     CDS             complement(18084..20213)
                     /gene="pnp"
                     /locus_tag="VBC52_000490"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEO6882095.1"
                     /translation="MSKQVFEMIFAGKKLVVETGQVAKQANGSVVVRYGDSTVLTAAV
                     MSKKMSTGDFFPLQVNYEEKMYAAGKFPGGFNKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSFDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVAYVDGNFI
                     INPTAQEQEASALELTVAGTKEAINMVESGAKELSEEIMLEALLKGHEAVCELIAFQE
                     EIVTAIGKEKAEVELLQVDPELQAEIIATHNIALQAAVQVEEKKAREAATEAVKEVVI
                     GEYEARYAEHEEYDRIMRDVAEILEQMEHAEVRRLITEDKIRPDGRRVDEIRPLDAEI
                     DFLPQVHGSGLFTRGQTQALSVLTLAPMGEAQIIDGLTPEYKKRFMHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIEGITPQILEEALAQAKKARFEILDVLHGAIAEPRPQLAPTAPKIDMIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDEEGNVSIFSSDQAAIDRTKDIIASLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTANVADVLEIGEEVDVKVIKI
                     DDKGRVDASMKALLPRPPKADNPKKES"
     gene            complement(20594..20863)
                     /gene="rpsO"
                     /locus_tag="VBC52_000491"
     CDS             complement(20594..20863)
                     /gene="rpsO"
                     /locus_tag="VBC52_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEO6882096.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNDHIK
                     QHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            complement(20951..22210)
                     /locus_tag="VBC52_000492"
     CDS             complement(20951..22210)
                     /locus_tag="VBC52_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEO6882097.1"
                     /translation="MRAWKGIVLILSSIVVTLVAWQNAGLSEFVVPGLALTSLSLTFL
                     LSTKFRILESYFQGIENMYFYHKVMAVFSMILLLLHKIGLGQGGHGSEFAKTIGSAGL
                     YLFLSIVFVAYFGNFLKYEIWRFIHRFVYLAYILGLVHTFMILGDRILGNTLLSLIVL
                     GYAVIGVISGFYIIFLYSRMRFRRVGYVQKVTHLNHDTTEIEIAMKRPYRYDYGQFTF
                     LKIYQAGFESAAHPFSISGGHDRVIFLTVKASGDYTKSIYKQLKVGTKIALDRAYGHM
                     LFDKDKKEQVWIAGGIGITPFISFIRENSILTKRVDFFYTFSNQDNLIYQDMLESYAK
                     ANPNFKLHLNNSSLKGRLDFSQSVFEGQPTIFMCGPTSMTSTYAKVFRQKDAKSRLVY
                     EGFSFRDSWLSIFLLKTFDKVYSNLIK"
     gene            complement(22319..23248)
                     /locus_tag="VBC52_000493"
     CDS             complement(22319..23248)
                     /locus_tag="VBC52_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEO6882098.1"
                     /translation="MRCSTKEMRLVYRDFLLQANQENKQITVLEADLSSSMSTNALAS
                     EFGKRYINLGIMEAEMVGLAAGLAIKGYKPYLHTFGPFASRRVFDQVFLSLGYSQLSA
                     TIIGSDAGISAEMNGGTHMPFEELGLLRLIPKATIFEVSDDIQFEAILKQTLSIDGLK
                     YIRTIRKAPTAVYEGREDFSKGFIQLRQGKDITLVASGIMVSRAIEAADYLKELGIEA
                     SVIDLFKIKPLPEELKPLLIDQSIVTIENHNRIGGIGSALCEWLSMEKDTTVSRMGID
                     ERFGQVGQMEYLLEEYGLAVKDIVQHCKSIYKS"
     gene            complement(23245..24102)
                     /locus_tag="VBC52_000494"
     CDS             complement(23245..24102)
                     /locus_tag="VBC52_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO6882099.1"
                     /translation="MTSPEERYSSLRRFATEIRLNTLETLNHLGFGHYGGSLSIVEAL
                     AVLYGDIMDINPEKFKESDRDYMVLSKGHAGPALYSTLYLKGFFDKTFLHSLNTNGTK
                     LPSHPDRNLTPGIDVTTGSLGQGISIATGIAYAQKIENSSYYTYTIVGDGELNEGQCW
                     EAIQFAAHHQLHHLIVFVDDNKKQLDGLTADICNPGDFVAKFEAFGFDAVRVKGDDIE
                     AIDKAIKTFQDSNSVRPKCIVLDSIKGQGVKELEELASNHHLRPDLQQKTMLERALIS
                     LRESLEVVE"
     gene            complement(24105..25460)
                     /locus_tag="VBC52_000495"
     CDS             complement(24105..25460)
                     /locus_tag="VBC52_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEO6882100.1"
                     /translation="MKGLLDFLVNIASTPAILVALIAIIGLVLQKKGVPDIVKGGIKT
                     FVGFLVVSGGAGIVQNSLNPFGKMFEHAFHLVGVVPNNEAIVAVALTKYGSATALIML
                     AGMIFNILIARFTKFKYIFLTGHHTLYMACMIAVIFAVAGFTSFSLILFGGLALGIIM
                     SVSPAFVQKYMIQLTGNDKVALGHFGSLGYWLSGFIGGIVGDKSKSTEDIKFPKSLSF
                     LRDSTVSITISMAIIYLIVAVFAGEAYIAKEISNGVNGLVYALQLAGQFAAGVFVILA
                     GVRLILGEIVPAFKGISEKLVPNSKPALDCPIVYPYAPNAVLIGFISSFVGGLVSMIV
                     MIVTGTTVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFVQGILISFLPIFLMPVL
                     GGLGFKGSTFSDADFGLTGIILGALNHVGGAIAIVIGIVVILIGLFGISFVGKSTHKE
                     G"
     gene            complement(25473..25757)
                     /locus_tag="VBC52_000496"
     CDS             complement(25473..25757)
                     /locus_tag="VBC52_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6882101.1"
                     /translation="MLRIGTACGSGLGSSFMVQMNIESILKDLGVSDVEVEHYDLGGA
                     DPSAADVWIVGRDLEDSAGHLGDVRILNSIIDMDELRELVTGICQEKGLI"
     gene            complement(25760..27796)
                     /locus_tag="VBC52_000497"
     CDS             complement(25760..27796)
                     /locus_tag="VBC52_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEO6882102.1"
                     /translation="MVDNKTVVIMLVFLARKNLSLYELTVQTKFSIKVIIEQINYLNS
                     FLAKNHLPAIAHSAGRYQLLGDEKEHDKIVSLLEAEQFYLTQEERVCLIYLYSFCRRE
                     FVSNVHYQDFLKVSKNTTLSDIKMLRSKLAKRGISLTYTRAKGYSLVGDEMDKHQVAF
                     QMITQLLESPIGFWSLNYILSSWKFALSYEKLEKTVEYFYESFQLSPIQDRLEKSLYF
                     IILILCRYQRSVDRVLQGSPIVSEQLKELTTIIVTNLSQDISLSKPLDQKEKDYITLI
                     LSGCFEGEGTKDDDFFEALAKAIVDEMETVSLLNFSNKEELLQGLKRHIIPAYFRLKY
                     GLTGDSGYTQNIKEHYSDLFLLVKKALRPLEEQVGLIPDSEISYFVIHFGGYLRQSGG
                     TQSMSYKALILCPNGVSSSLVIKEKLRGLFPQIHFHRVSKIEQLKLIDNQTYDMVFST
                     IFVETKKPNYLVSLMMTAEQVQQLKELVISDFPKACLDDFQLDQLIATIKKYAHVHCE
                     EELKLALRTMVKQDIFRKDVRPLLHQLITEETYQTSSEQMNWKEAIRLAAKPLLASGK
                     ITESYPEAMIEKVEEFGPFINLGKGIAIPHARPEDGVNSVGMSMLVLEQPIYLLDDPK
                     QEIYLLLCIAAIDNETHLKALSHLTTILRDNNNVKALLASRGYQDIEMIIKQED"
     gene            complement(28012..29637)
                     /gene="treC"
                     /locus_tag="VBC52_000498"
     CDS             complement(28012..29637)
                     /gene="treC"
                     /locus_tag="VBC52_000498"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEO6882103.1"
                     /translation="MTIDKRKVVYQIYPKSYKDTTGNGVGDLRGIIEKLPYLAELGID
                     MVWLNPFYPSPQRDNGYDISDYTAINPDFGTMDDFEEMIEVGRQYRIDFMLDMVLNHC
                     SIEHEWFKKALAGDRYYQDFFILRDNPTDWVSKFGGNAWAPFGDTGKYYLHLFDITQA
                     DLNWRNADVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEILENCPINDGKPAYTDRP
                     ITHDYLKMLNNASFGQDDSFMTVGEMSSTTIANCILYTAPEREELSMAFNFHHLKVDY
                     KDGQKWTIMAFDFPALRDLFHSWGEGMSEGNGWNALFYNNHDQPRALNRFVDVKRFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDYSSMDDYVDIESLNAYQIMLDEGKSQE
                     EAFSIIRAKSRDNSRVPMQWDDSTNAGFSEGAPWLKVGKSYKEINVAKEKTGLIFTFY
                     QELIRLRKQLPIIADGNYKAAFKDNEKVYAFERHLDKEKLLVLNNFFAEKVKIKLPEN
                     YLQGQVLLSNYKDVTLDETVTLQPYQTLAILVS"
     gene            complement(29859..31889)
                     /gene="treP"
                     /locus_tag="VBC52_000499"
     CDS             complement(29859..31889)
                     /gene="treP"
                     /locus_tag="VBC52_000499"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEO6882104.1"
                     /translation="MEQFKHDAKALLEAIGGKENISAVTHCATRMRFVLNDSSKAKVK
                     VIEELPSVKGTFTNAGQFQVIIGNDVPIFYNAFVAVSGIEGVSKEAAKSAAQKNQNPL
                     QRVLTMLAEIFTPIIPAIIVGGLILGFRNILDAVPFEFLGQKVVDGVRQVDSSGHPIW
                     NTLVDVSTFWSGVDSFLWLPGEAIFHFLPVGIVWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYSVASTSAADIAKNWSWNFGYFTVQKIGYQAQVIPALLAGLSLSYLEIFWRKHI
                     PEVVSMIFVPFLSLVPAIILAHTVLGPIGWTLGKWISAIVLIGLTGPVKWLFGAIFGA
                     LYAPFVITGLHHMTNAIDTQLIADTKTHTTGLWPMIALSNIAQGSAVLAYYFMHRHDE
                     KEAQISLPAAISAYLGVTEPALFGVNVKYIYPFVAGMIGSSVAGLLATTFNVQANSIG
                     VGGLPGFLSINVKYMGYFFICMAVAIFIPLFLTLFFKKSGILTKTEEEKLVPDAVIAS
                     TTETKSAKEKAVVSGTKLSVVSPLSGLAKPLDQASDPVFSQGIMGKGVVIDPSDGELV
                     SPVDATVSVLFPTKHAIGLLTSEGVEFLIHIGMDTVNLEGKGFTSHVAQGDTVKVGDK
                     LITFDIPMIKEEGYIVETPILITNQQEFRPEELIDLPKQIKRGQALMVAKKI"
     gene            complement(32172..32798)
                     /locus_tag="VBC52_000500"
     CDS             complement(32172..32798)
                     /locus_tag="VBC52_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6882105.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEINLEVAPGQVLGIFGQSGCGKTS
                     LSRVLAGFLHPKSGEVLVDGSNLPSKAFRPVQLIQQHPEKTMNPLWPMKKSLEEAYYP
                     SRDLLDAFGIQEKWLNRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSMLEIVKDRNLGLIVISHDFAMLEKLCNQCYMIEENRIVSFNGD"
     gene            complement(32782..33585)
                     /locus_tag="VBC52_000501"
     CDS             complement(32782..33585)
                     /locus_tag="VBC52_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6882106.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEIKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNASVTGDMIYRGQSLNSKRIKQLRGKDITLIPQSVN
                     YLDPSTKVKHQVRLGISENSKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDKVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGNGEQLQTEFARSLWRSLPQQEFLKGVTHDLRG"
     gene            complement(33597..34418)
                     /locus_tag="VBC52_000502"
     CDS             complement(33597..34418)
                     /locus_tag="VBC52_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6882107.1"
                     /translation="MILRRRTIVLWQLGIAISLILSILALNLYFHSTPLQTNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVILATVFGVLAGLGNSIID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYHLKN
                     KEFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(34415..35392)
                     /locus_tag="VBC52_000503"
     CDS             complement(34415..35392)
                     /locus_tag="VBC52_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6882108.1"
                     /translation="MKHITTVVIWKILRCLSLVFGVSILTFILLKQSPVDPVMASVNY
                     DTSLTPVQYKAIAHHYGLDKPAPVQYFIWLKNFIQGHLGTSLVYRQPVIDIIRSRAGA
                     SFILMGLSWVLSGLIGFILGTLSAFHQGKLLDRIVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRIKHLILPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETEWQIFKNHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            complement(35505..37133)
                     /locus_tag="VBC52_000504"
     CDS             complement(35505..37133)
                     /locus_tag="VBC52_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6882109.1"
                     /translation="MTKYLKYISFVALFLASIFLVACQNQNSQTKERTRKQRPKDELV
                     VSMGAKLPHEFDPKDRYGIHNEGNITHSTLLKRSPELDIKGELAKKYKISKDSLTWSF
                     DLNDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQVNIHLTEAHS
                     TFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELASKKVKGTRLLDIASNDVRGLSLPYVKK
                     GVVKNSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDRTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGNLKAEFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHYPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSPDLDKANKYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINVGKQGVHSHGHDWSLLTNIAEWTWDESAK"
     gene            complement(37376..38104)
                     /gene="lrgB"
                     /locus_tag="VBC52_000505"
     CDS             complement(37376..38104)
                     /gene="lrgB"
                     /locus_tag="VBC52_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HEO6882110.1"
                     /translation="MELLKTPIFGICFSLILYTIGQHLFKKSKGFFLLQPLFFAMVSG
                     IVILWLMSKGLGTDVKTFYTQAYKPGGDLIFWFLNPATIAFAVPLYKKNDVVKKYWVE
                     ILSSLVIGMIVSLILIVAISKMVGLSQVGIASMLPQAATTAIALPITAAIGGNTAVTA
                     MACILNAVIIYALGKKLVSFFHLNDSKIGAGLGLGTSGHTVGAAFALELGELQGAMAA
                     IAVVVIGLVVDLVIPIFSHLIGLL"
     gene            complement(38106..38561)
                     /locus_tag="VBC52_000506"
     CDS             complement(38106..38561)
                     /locus_tag="VBC52_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO6882111.1"
                     /translation="MKHTSKETKTKEAPMFIQMSIYAAILLVSQMISMLLPKSLPIPT
                     TVIGLVLMYVLLTAKIIKVEWVDSFGALMISMIGFMFVPSGISVAANLDILKAEGLQL
                     VAVITISTVVMLVVVAYVARLILAIRELSVTDLFKKILPNHYSKKVEEK"
     gene            complement(38731..39471)
                     /locus_tag="VBC52_000507"
     CDS             complement(38731..39471)
                     /locus_tag="VBC52_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEO6882112.1"
                     /translation="MKILILDDEMFARQELSFLVEHSQEVDNPEIFQAEDISEAEKIL
                     FRQQIDLIFLDISLSEENGFTLANQLSQLAHPPLVVFATAYDNYAVKAFESNAVDYIM
                     KPFEQQRVDMALSKVKKLSQLTTASDVEQAIPKKASVELLTLTLSDRSVVVKMQDIVA
                     ASVEDGELTVSTVQKTYTIRKTLNWFKSRAVAPYFLQIHRNTVINLEMIEEIQPWFNH
                     TLLLIMSNGEKFPVGRSYLKDLNEHLTM"
     gene            complement(39452..41197)
                     /locus_tag="VBC52_000508"
     CDS             complement(39452..41197)
                     /locus_tag="VBC52_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor protein LytS"
                     /protein_id="HEO6882113.1"
                     /translation="MLMVLLFQRLGIIMILAFLLVNNSYFRQLIEERSKRETVVLVII
                     FGLFVIISNITGIEIKGDRSLVERPFLTTISHSDSLANTRTLVITTASLVGGPLVGSI
                     VGFIGGVHRFFQGSFSGSFYIVSSVLVGIVSGKIGDKLKENHLYPSTSQVILISIIAE
                     SIQMLFVGIFTGWELVKMIVIPMMILNSLGSTLFLAILKTYLSNESQLRAVQTRDVLE
                     LTRQTLPYLRQGLTPQSARSVCEIIKRHTNFDAVGLTDRSNVLAHIGVGHDHHIAGQP
                     VKTDLSKSVIFDGEPRIAQDKAAISCPDHNCQLNSAIVVPLKINDKTVGALKMYFAGD
                     KTMSEVEENLVLGLAQIFSGQLAMGITEEQNKLASMAEIKALQAQINPHFFFNAINTI
                     SALIRIDSDKARYALMQLSTFFRTSLQGGQDREVTLEQEKSHVDAYMNVEKLRFPDKY
                     QLSYDISAPEKMKLPPFGLQVLVENAVRHAFKERKTDNHILVQIKPDGHYYCVSVSDN
                     GQGISDTIIDKLGQETVAESKGTGTALVNLNNRLNLLYGSVSCLHFSSDKNGTKVWYR
                     IPNRIREDEHENFNS"
     gene            complement(41224..41868)
                     /locus_tag="VBC52_000509"
     CDS             complement(41224..41868)
                     /locus_tag="VBC52_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6882114.1"
                     /translation="MEKVIIFDMDGVIVDSEYTFLDNKTEMLREEGIDTDVSYQYQYM
                     GTTFEFMWQAMKEEFGLPKTVKEYIAEMNRRRQAIVARDGVRPIKGAQRLIHWLHQHG
                     YRLAVASSSPMVDIKRNLKELGVTECFEYMVTGEDVSSSKPAPDVFLRAAELLDVDPK
                     VCIVIEDTRNGSLAAKAAGMYCFGFANPDYPPQDLSMADKVISDYQDIYIYLPE"
     gene            complement(41992..42387)
                     /locus_tag="VBC52_000510"
     CDS             complement(41992..42387)
                     /locus_tag="VBC52_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO6882115.1"
                     /translation="MYNKVIMIGRLTAKPEMVKTPTDKSVTRATVAVNRRFKGSNGER
                     EADFINVVMWGRLAETLASYGTKGSLISIDGELRTRKYEKDGQTHYITEVLASSFQLL
                     ESRAQRAMRENNVSGDLSDLVLEEEELPF"
     gene            42468..43184
                     /locus_tag="VBC52_000511"
     CDS             42468..43184
                     /locus_tag="VBC52_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO6882116.1"
                     /translation="MTNVTSYKEMLAKPWGKIQYEITFAQLSHIKNQNVLDFGAGFCL
                     TEQHLAKENNVTAIEPNPKLLYDNQSDNIYKILGSYEALRDLPDQSFDTIICHNVLEY
                     IDKHNHPAYFDEFSRLLKPNGELSLIKHNITGKILQSVIFSNDTSTAMELLTGEANFK
                     SASFDQGNIYTLEELKQNTNLLVERYQGIRTFYSLQPNHFKTETGWLNKMLAIELSVA
                     DKAPYKDIAFLQHITLKKSL"
     gene            complement(43238..43864)
                     /locus_tag="VBC52_000512"
     CDS             complement(43238..43864)
                     /locus_tag="VBC52_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEO6882117.1"
                     /translation="MIFTYNREHVGDTLMVIVKDSQGAKLDVDRRGQVARVYLQDSKE
                     TVAWNIFEVSSLIVIEGAGQITLSDQDIKILNAELLKEGFEDSLVNNIEPTFVVAQIK
                     EMIDHPDSDHLHICQAEINDGKTVQIVCGAPNASVGLKTVAALPGAMMPNGSLIFPGK
                     LRGEDSFGMLCSARELALPNAPQVRGIIELSDQVIVGESFDANKHWKN"
     gene            complement(43897..44220)
                     /locus_tag="VBC52_000513"
     CDS             complement(43897..44220)
                     /locus_tag="VBC52_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEO6882118.1"
                     /translation="MILPESYEEIAAYIDSTKKVVFFFTADWCPDCQFIYPVMPSIEK
                     DFSDFVFVRVNRDDYIELAQQWNIFGIPSFVVVENGQELGRLVNKNRKTKAEITKFLA
                     EINYK"
     gene            complement(44217..44501)
                     /locus_tag="VBC52_000514"
     CDS             complement(44217..44501)
                     /locus_tag="VBC52_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEO6882119.1"
                     /translation="MKNKKILFGTGLAGVGLLAAAGYTLTKKVTDYKRQQITQTLREF
                     FSQMGDIQVFYFNEFESDIKMTSGGLVLEDGRIFEFIYRQGVLDYVEVSK"
BASE COUNT        14418 a         8931 c         7084 g        14164 t
ORIGIN      
        1 acatactatg caagttattt tacctgatga acaagttaaa gaaattcaat tattagtttc
       61 aaatgttatc aagtcagaaa tacaacaatt taaaactgat attggtcttg aaaaaccata
      121 tttgaataaa ctccaaactt gtcagtattt aggtatttca aataatactc tagactcatg
      181 gattaagttg ggtttacctg taattaaaat aggaaaaaca attagatttg ataaaaatgc
      241 aataaatagt tggctacaaa gccactaaaa ttctaccatg gttctttaaa tgaccatggt
      301 ttttagtaaa ggtaaaaagg atagtaatat gacaattaca aaaaccaaga atggcactta
      361 tcgcctgaga gtatatatac cagaagaagc aaaaaaatca ctcggtattg ataaaaaatt
      421 aatagaaaaa aggtttaaat taagaagtga tgcaaagaaa tatgaattag aacttcaaaa
      481 taaaattgat aatgtactca atggttcgtt tcaaaatatt catgatactg gtgatatatt
      541 attctcagaa ttctacaaaa atatttggtg gaattcttat aaatctggtc aaaccactac
      601 tacaaataaa ccaccatcaa aagttacaat agataatact gaaattgttt ttagacttca
      661 tattttgcca ttgttgggta attattcaat aaactttttg aatcagaaca aacaagtagt
      721 acttaatcta ttaactaaaa aagcagaaga atatgcgaat ttcaaagtaa taagaagtta
      781 tgtcaattca atatttgact gggctgaaga acttgaatat attgaagcaa atcgagtttc
      841 aaagacaatt aagagaataa aggctactaa aaaaataatg ttacaagaat caaaagaaga
      901 ttcagactta tttttatctc aaaaagaact attagaatgg tttaaagcgt ttgaagaaga
      961 tcttttaaat aataaaattc tttttaagga ttatgtttta ttttatacaa cctttttcct
     1021 tggagatagg aaatcggaaa cttatgcttt acagtggaaa catatcgact ttaatagtcg
     1081 tcaaattcaa ctagtacaag ccctagataa atacaaaaac aaaaaagcta caaaaggaaa
     1141 taaaaaaacc acctttaata tccctcttga attacaggaa ctacttattt cttggaaaaa
     1201 tcaacaaaaa ttggaactca gtaaacttgg aattagtcaa acaaatgatc aatatgtttt
     1261 tacttatata gataccaaag gaaacttaaa tagtcctctt catactgatt acttaaataa
     1321 taagatgaaa tcagttaata gaagacatcc tgagctaaag catgctactc cacacaaatt
     1381 aaggcatact ggagcaacac ttgctaaaca agctggaaca tccttggagg acatttctca
     1441 agctttaacg catagtgata ccttaattac taaaacatat gttaatacat caaacatagt
     1501 tcctatgact gttggtgaaa tagcctttag aaatttaaaa aacgaatagt gtgaattttg
     1561 ggggaaaaat ggtgtgaaaa atcataaaca tttacaaaaa aacaccccat ggtgtgaacc
     1621 atgaagtgtt tccaaatgtt gatatataag tgtatattaa cgttttgaga actgacttgc
     1681 tttacgtgct ttcttaagac ctggtttttg gttcactcca ttatttaaag tcaaatccct
     1741 ttaaaatcaa cgtttctaaa ataaaaaatc actgaaattc ttatttatag acaaatattt
     1801 aaacctaaac ggtatcaaaa aggtatcaat tttctgtagt ttcggagctt ataaaataaa
     1861 cgaaagctat ctcaatcaat tgggattttt ttattgactt tttttatgta tatgagataa
     1921 ttaaataaat taaataaagg agttacataa tatggttaca gcagaaaaaa atagagctgt
     1981 cacattccaa gctaacaaag aattggtaag cgaagcaatg acagtattaa acaagaaaaa
     2041 tttaacctta tcatctgctt taagattatt ccttcaaaat gtcgttgtca caaatgaggt
     2101 tgacttattg acggaagagg agctagagaa agaaaaactt ttcaagcaat ttcaagctga
     2161 aatcaacaaa aatattgaag atgttcgtca agggaaattt tatacctctg aagaagtgag
     2221 gtctgaactt ggactataag aaatatcaga ttatctatgc tcctgatgtt ttagagaagt
     2281 taaaggaaat tcgtgattat atttctcaaa actattcctc gacatcaggt cagcacaaaa
     2341 tggagcaaat cattagtgac atagaaaaac tcgaggtttt ccctgaagtt ggttttgatg
     2401 ccgatgaaaa atatggttca aaaatcagta agtaccacag cactagaggt tataccttaa
     2461 gtaaagatta tattgtccta taccatatcg aggaggaaga gaatagggtc gttattgatt
     2521 acttgcttcc tactcgaagc gactatatga aattgtttaa atagcaatca aaaagcctta
     2581 gcgtaatacc aaggcttttt gaagattaca aattaactag gagcaaaatt agttcgggct
     2641 gtttagtaga tattccacaa ggcagagcca accctgtatt gacaaacaat acctttgaga
     2701 aaactgttac cagtcaatca accttctcct aatgtaaaag ctgagaaccc aatcggggat
     2761 ttacctaagt atactcagga ggcaacacct ctacctaata ccttcaataa gtattttttg
     2821 aatgaattgt taccaattca aactcagtct caactttctt ccactctatc actttttatt
     2881 accttttgca aggcttttat aaacattaat ttgttcaatc ggtagcattg tgtccatggt
     2941 tttaattagt ctatgataga ttgcgtaacg tgtgtcagga ttggaaatat cagcaataaa
     3001 atttctatat cccttaacat aatcttcttc atcagttggc aaaataccta tcagcatctt
     3061 tgtactatct tttggattaa cctcatatcc aaatctgata ttgtaattag ctagttcatt
     3121 ttttatacga taaagctcta gaccattatt gtaaaaatca ttgaattctg ggacttcaaa
     3181 caatgacgta tcaatgtcta aattgtcttc tgccacataa gaatatgtcc accaaagtag
     3241 gaataaaaga tttttaccgg tgtaggtagg tcctactcct cccatatctt tttttgataa
     3301 gtcatggtca aagtaataaa caattttttt atctgtcatt ttaatttctc cttgctcatt
     3361 tattccatgg ttctcttgac aacaagtaac ctaggctgtt ttagcctatg gcgatcggtc
     3421 gccagttctt tagcctgacg actcgacccc atctagtcta aacttgccta gcttgttatc
     3481 aagagacttt cacggcataa aagtggctaa ctattactat caaccatctt cttaacttgt
     3541 ctgattgctg ttttagctaa accgatagga tttagagagt ttatgattga taatgtttct
     3601 ctataaacct gtttaggcat aaattcttta gctttttctt tccaaacaga gaggttttta
     3661 ttcagaattt tcacttgttc atcaagcatt tttactttgt cctttaattt tctgttttct
     3721 tgacttaatt tgatattttt atcagctgtc ttacggtgat tgagtaattc tctagaaagg
     3781 tgattgttct gcgactgggc tttaaccaag tctcttttta actcagcatt actagatgca
     3841 tgtttcgaag cagtttgaaa tagtctatta taggtttctt tatccatctt caatttgcca
     3901 tcaaagcttt gttttaccaa atcttctttt tgaattctat caagttgtaa tagttcaatg
     3961 tcttttatgt cttcaatact ttcagctagt gactgtttct ctaattcata ctgcttcaaa
     4021 tcgccaacag catcccattc tttcgcttcc aattctttaa gcctactttc tttatcagct
     4081 attgtatcgt taacatttga aatcttatca tcaagtgctt tcaattcttc atgtttatca
     4141 gctagttttc tatcagcgtt atccagtaat cgttgttttt ctttaaattc agcagtttcc
     4201 aaatgttttt tcttactgcc tatttcgcca cgttgtaggt taaagccatg ctcattaaga
     4261 tacttgggaa gttcatcttg gattgctaca agtttctctt tatttccaaa cagtgctttt
     4321 gaacttaact taccattttt cattggcacg atacctaaat gcatatgcgg agtactttca
     4381 tcaaggtgaa ctctagcgta agcaatatta gcatcaccat atttttcagc aaaataatct
     4441 ttagctgtct caaaaaactc tcgtgtctgt ttctcatcta acgagtcaaa aaacgtttta
     4501 tctgaagtaa taatccattc gttacagagt atagcatctt tacggactcc acgagtagat
     4561 aagcgatttt cattgatgtg ctcctttatt tgtttgtgat aattttgtgc ctggtctcga
     4621 tttgtcagtt cataattaag gtgagatttc tcaacgtcaa tttctttatt tgagtgattt
     4681 ttaaaaatcc gttcattatg attataaata gccgtcaact gacctgactt gtacttagcc
     4741 attctagcaa caacataact catttttaat tctcctttct cggctcaaaa ggtcgccgag
     4801 atggtggcgg taattgccac catgaataag caacctatta tctggatttc ttcttaaggg
     4861 atcggcataa cttccatgta aagtgtaacg cactacactt tactacgtaa atgtgcgttc
     4921 tccgaagggt cttgcagaca gctcttcgct ccgaatacag gagcgacttc gaaccacctc
     4981 tcgagccaca agaggagctt cgattatact cgccgaagcg agtagagcac ttaagcagtg
     5041 cccttggtcg gacgcttcgc attgtccgac aattgccatc aaaagatggc aagccatatt
     5101 gccactaata aagtgattac catgacagct aaagcaataa tcagcaatag cttctgtcgt
     5161 tccaagctcc gaacatagtt gagtaactca taattttttt gtaccaagtt ttggttatcc
     5221 tcaagtaaag gagttaactt atcagctagt ctatctatcc gatattgcat tcccttcttt
     5281 tcttgctcga attcttcttg aatatcaccc agcgtttttt ccagttcaat gacatcattc
     5341 tcaaaatgat aatcttcaag tattttaatg cctatcaaat caagattttc aaccttttgg
     5401 ttgtctcggt ataaatgacc tttcaacctt tctttatatt ttggagagcg tatgcgttta
     5461 tagatagatt ggctactaac tcctagacta tcagcataat cttttattct catttttgac
     5521 ctcgtaactc cttgataata ctgaattttc aacaaaaggg ttgaaagcta tttttgtcta
     5581 tcctaccccc ataataagcg agctgatttt ctagtcttgg tctttcatgc ttcaacaagt
     5641 ctttcagcaa acctaaataa tcagtatctg atgtttgatg agtttctaaa atttttttgt
     5701 atcttgctct tgtatttcta tggagttttt tccagatatt tttatcgtga ataagagcag
     5761 ttagatagat ttgggtctca atagataagc ctttaagatt gaatggttta aaaataatag
     5821 tatcaataac ctcgaatatc tcatcaattg aacggcttct taattgaaat tccaagcgcc
     5881 accagtgctt aaattgactt gcaaagtctt tatctttatc agtaccattt tggagttgtt
     5941 cctttttctt gttatatagc ctaacttgtt tttcagaacg tggagcacct agatattttg
     6001 tttctaacgt tccagtacta gaacgaaatt ccttggttgc ggttggtctg cccttttttt
     6061 cgagcacaaa ctcactacaa tcaacctcaa atagatcaaa agctaaatca gcacgtgata
     6121 ttgaaatatc ttcaagaaat ggtataatag tatctataat ttcactgtca actaaacgga
     6181 gtttattggg attaaactcc aagcgaaatg gacgagcctt gcggtcttgt cctttttgtt
     6241 tgtcataatt catatggagc acaacgtgga ttccatcccc agtggtggct cgaaacatgt
     6301 cagtcatcgc agtttgaaca gtaatagcta aatgggtttc tatgagttgt ttcatagcta
     6361 aatacttttt agatttgata taggctgtca tagtgatatt gtctaagctg acttttaatt
     6421 tcaaacaaac ctcctttctt tttggcggtc gcctaacggc gccgattttt ttgtctgcgc
     6481 aaaggcctac gtaaaatgac attcttgggg gagtagcccc aagccgaccc cttcggagtc
     6541 ggaccatttt cattttgctc cagccaaact tgccaaaaaa cgggtgtagt aaatgcactt
     6601 cagatgtgca catagcgggc tgttagaggg cgccctgtta taaatttttc agaaccccaa
     6661 taaatctgac ctcaaatctc tgttttcaaa atattttcat agttattttc ataaatattt
     6721 ttgactagaa attttgtcaa tccaacacag attttttgaa aaagtcttct cggtcaacca
     6781 aaaatatctt gaggattttt tcgataatgt catcaggcgg aacctgttta tttttcttgt
     6841 agctaaagaa acggtcttta gcttttttga tttctcctaa atcgtcggac atatcagcaa
     6901 acagtaactc attcagttca aaccaacgta gctcagattg cttacgtagt gactcaaagt
     6961 ttttccagaa tagacgtccc tcatctaact gctccatctt cctcatcagc tgaattcttt
     7021 caattttccg atattctttt ggcaagagta ccgtcacata gtcaagggac atgttcgaat
     7081 aaataacctc atctgtcaca acaatgggaa gcttcaaggt gctattgaca tcttttaaaa
     7141 tctttcgaac aaattgactg agggtaattt gtgtattcag cttgtatttc aacatcttag
     7201 tgcttagtaa ttcaaatata tcggtcgacc tgaatagcca ttccatcagt gttttagcat
     7261 ctatatcttt gcttgagaat gcttgatagt caatctgata gctatccaat cgcgactgaa
     7321 tagcttggtt aagtgttata tcttttccaa aataaatctt tatcattgct tatacctcgt
     7381 tgatataggt tttgtagggt aaagagtagc cataaaaaac atctactctt tctcgttcaa
     7441 tcaaattaac ttcttccagt cgttgcataa tatctatcat tgtcgttctg ctgcaagaaa
     7501 acattttagc taattcaatc agtttaaaat ttagatatat actgccatca gtatctatcc
     7561 aacctttttc aggagcttct atctgtctat ctttcaagac agcatagact aaaatatctc
     7621 tcgcagatag actactgtag aaatcatctt gcagtaaaac tttcgggata agatagctgt
     7681 cgttatcata gtccatagtt cttacctcct atctctctcc acaccatatc ccaagtagaa
     7741 gcaagggaac atgcaacaca aaagttaaag ctcccaaaaa ccaacgttca actaaaaaga
     7801 aaaatattga tagtatcagc acaatataag tgaacaatcc aacatcaaga aagattgtcc
     7861 catattccaa acgaagacca ccaaaaccat ttctaagtat cttttttcct gtcaagtctt
     7921 ctaaattatt tttatccatg ccaaatttcc tatctttcaa attgttgtag ccattgagcg
     7981 accttgcgct tatcgtacag agtcactccg tcaataacca tatgtggcat gcctgccttt
     8041 tgccatttga caatagttgt cgttgagaca tctaaaaaac ggcacaagcc agccatacga
     8101 accataggct tatatagctc tttgtcctca agggcttttt ctattgcttt ttgaaccata
     8161 gtgtcatagc gagccagtaa cttcttttca gttactttgg ggagctgaat ggtaaatcct
     8221 tctgtcattt tgagttctcc tttttgttaa aatcgatttt aagctctgaa acgcttattg
     8281 taatcaagta tttcagaatt taaatatctt cctagggtaa ataacctgag aaggttttta
     8341 atctgttatt tgtgattgta gaatcatatt tcagacttag aatgtaatct tgccattctt
     8401 tttcttgctt cctctcgttg ttcttcagtc atttcacgtt taactcgttt tcgaacaaat
     8461 ggagaaacca tataatcatc aagatttgtc aatttagcat gaatccatat aatgcggtcg
     8521 tcttctttct cttgacctaa aatctgacaa ctatcaatag ttttcatgat tttagtaatg
     8581 tgtttcctaa cattactctc aaaataccaa cagttatcca tttcatcata acggacaatc
     8641 gtttctcgtt cttctagtgt gtaagccata actgacccct ttctttattt tataagcttc
     8701 gaaaacaaat tactcttttc gtgtaattta tattaaaatt aaactctttt tgagtttttg
     8761 tcaactattt tttctctttt tgtgtaattt gtgttataat atcccaaaaa ggagaataca
     8821 taaatgacaa attttcaact tcttgtaaaa gaaagcaata aaagtttgac tgaaataagt
     8881 caagaaacag gcattagtta ttcaacatta ggaaattata atcaagggac tagaactcca
     8941 aagaaagaga atgctaaaat ccttgccgac tactttgatg tctccgtccc ttatctatta
     9001 ggacttgacg acaatagaca aatactagat tttgctgggg aggggattgc aaaaggtatc
     9061 attgaaaaaa ttaaaggtcg aaagacactt gatatgcttt tgacctcaac tccatatctg
     9121 gaagaaaatg tagatatcat gaatgatatt attaataaaa aggttctaga ctcatacatt
     9181 gactttctgt atgaaggaaa atatcctcca gcagtcattg tatttatcaa agatgttatc
     9241 aagaatcgtt ttgatgatat tccagcacta gcaaacagaa gcagtgataa aaatagcgag
     9301 taccattttc tatgggaagc ttgggaaaaa gaaccaagaa aaaaagaacg aacggaacag
     9361 acaaggaaga aatggaaaga gcaaggtatt cctatattta aatcttggaa atagaataga
     9421 cagctaattt ctttatttta taagcttcga aacctaaaaa ataaaggaga aattacaatg
     9481 tccattcaca aatacccaag caaaaaagcc aaaaatggct atttgtattt agtcaaggtc
     9541 tatatgacta gagacgctca acgtgcagac catatcaaac gtggctttcg tactcgaaag
     9601 gaagccaaag actacgaagc taggctaatc taccttaagg cttcaggaag gttggaagag
     9661 tttatcaagc caactcataa aacttataac gagatattcg agaaatggta tcaagcctat
     9721 caagatacgg tagagccgac aacagcttca cgtactcttg atatgttcag gcttcacatt
     9781 ctgccagtca tgggagactt acctatcagc aaaatctccc ctttagactg tcagaacttc
     9841 atcacagaaa aggctaagac ttttaagaat atcaagcaaa tcaagtctta tactggaaaa
     9901 atatttgact ttgcaataaa gatgaaattg cttaagcata accctatggc agaaattatt
     9961 atgccgaagc ggaaaaagac acagatagaa aactattgga cggttcagga actaaaagag
    10021 tttctagcca ttgtccttca ggaagagcct tataagcatt atgcactgtt tagactacta
    10081 gcttatagcg gcttaaggaa aggcgaacta tacgctctta aatgggctga tatagacttt
    10141 cagagtgaga cactaagtgt caataagagc ctaggacgat tagacggaca ggcaatcgaa
    10201 aaaggcacta aaaatgattt ctcagtaaga aagattaagc tagatagcga aactatttct
    10261 attttgcaag aatggaaagc aaattgccaa aaagaaaagg cacaactagc agttgcacct
    10321 ctttctatca agcaagattt tatcttcact tactgcacac ggtcagggtc agtagaacca
    10381 ctgcatgcag actatatcaa taatgtacta tcacgaatta tcaagaaaca tggcttaaag
    10441 aaaatcagtc ctcacggttt taggcatact catgctacct tgatgattga aattggagtt
    10501 gacccagtca acacagcaaa aaggctaggt catgcaagta gtcaaatgac tttagacacc
    10561 tacagccatt caacagctaa tggagaagat aggtccatca agcaatttgc ggactatctc
    10621 aaagcaaaat aacagtcaaa aaagatgtag aagtttttcc aaaacggtat caaaaaaggt
    10681 atcaaaagca aaaaacgatt aaaaaagcac cttgcaagag acttgcaaaa tgcttgtaat
    10741 cgttgatgta aagctgtttt taacgttttg agaactgact agctttacga gctttcttaa
    10801 gacctggttt tttacgttca accatacgtg cgtcacgtgt aagaagtcca gcacgtttca
    10861 atgaatcgcg gaaatctggg tcaacttcta aaagtgcacg tgagatacca tgacggatcg
    10921 cacctgattg acctgcatat ccaccaccta caacgttaac aaaaacatcg tatgaacctt
    10981 gagttgaagt aactgcgaaa ggttggttga taacaagacg aaggtctgcg tgtgggatgt
    11041 actcttctac atctttttta ttgattgtaa ttttaccagt acctgggacc aaacgaacgc
    11101 gtgcaacagc gtttttacgg cgaccagtac ctgcatattg tgcttgtgcc attatataat
    11161 gctcctttcc tcttagataa gtcctgagat atcaagtact tctggttgtt gtgcagcatg
    11221 tgtatgctca ccgccaacaa atactttcaa tttcatacct tgtgcacgtc caagagtgtt
    11281 atgtggaagc atgcctttaa ctgatttttc aatcaaacga acagcatttt tagaacgaag
    11341 ttcacctgct gagatttgtt tcaaaccacc tggatacatt gagtgagtgt agtagatttt
    11401 atctgacgct tttttaccag ttaatttaac tttttcagca ttgataacaa tcacaaagtc
    11461 acctgtatca gtgtgtggtg tgaaagttgg tttgtttttt ccgcgaagta cgctagcaac
    11521 tactgcagaa agacgtccaa gaggtacatc tgctgcgtca acaacgtacc atttgcgttc
    11581 aacttggcct ggtttagcca tgaaagttgt tttgttcatg atttctccta tacgaattcg
    11641 tttttgttta caggtgtgga tgttccggtc cacgagagta tttgaaaggt tccggggcct
    11701 tacaaatggg gtaaacaata ccgcttataa tcatatcaaa ataaggtagc attcgtcaag
    11761 ttatttcttt tattttatca attttcatcc caaaataaat catctacagt tttattcaaa
    11821 gcttgacaca atttaatcgc ttaattatag tctccttttt caatggcggc tattgtctga
    11881 catgatacac cgaccttttc agctagagac tgttataata aatccatact agctctagcc
    11941 gtctttaagc gtaaattttt catattacta tctgtcctta tacttatggt agtacactga
    12001 aattcccata gctatcaatg atattcctat aactgctaaa attgcatcat ctccccttga
    12061 aaaataagta gcaatatcaa ccttcctagt aaaagcatca ataaaatagt tattcctatg
    12121 agggcaaatg cgccagcaac aaaaacattt tccaaaaact ttcatcaaca aacggagaga
    12181 cgctttttag attagaagtc gtaacaaaat agaaatacca ctaaataaag ttaatattat
    12241 tataaaatca ggtaacacac tccccttatt agctttccaa aaacactttc aagccataag
    12301 ataaaaaaca tcgtctaaaa acatttttga taactttgta aaaccaattg ttgttgtcgt
    12361 ttatcatatt gaggatagtt cttattttat tttttataat aggtctccta ctattttata
    12421 gtttcatagc atcatataaa atacatttta taaaatgtat actaaaagaa cataacgtac
    12481 tgttatgccc tttgatttgt tatcggtttt cttcccctaa agagaataga gctaaagcac
    12541 ctgagcccac atgagctgaa ataactggcc ctaatggacg aataataact tcttcaatac
    12601 gctcatctgc taataactgt tctttcataa cttgagctga atctttctct ccactataag
    12661 caataattaa agtgctataa gatagtgttt ttgttgcctg agtcacaatc tctttaatac
    12721 cttttttacg gccccttgtc ttggcaaatg gtactaattt accctcacta tctaacttaa
    12781 gtaatggttt aatcttagca acacttccaa tgatagctgc accttttgat aagcgaccac
    12841 tacgcattaa atggttgaga tcgtcaacta aaaagtatgt gtttaattta gggagaagtg
    12901 attcaatttt ttgtttcact tcttctaaag atttaccttc ttgtcgctct ttagttgcta
    12961 acatcgctaa gacaccctca ccacatgaag ctgccatcgt atccacaatc tcaatttgag
    13021 catcaggata tttgtctaag accatctcac gcgcaatagt tgcactctgg taagtgcctg
    13081 ataaatgtga agatagcgct aaatataaca aggcatggtc attttctgca taggttgaaa
    13141 aaacttcttc aaactgacca acgttaattt ggcttgttgt aggctttgct ccctcttgca
    13201 tacgctccaa caagaagtca ctagtaattt tttcgtctcc gactgtctca taagtctttc
    13261 catcaagttc aatggttaaa cctataatat cgacattgtg ctcttgagcc cacttttcat
    13321 ctaaatccga tgtcgaatcc gttagtattt taaaagtcat tgttacctca atctattatt
    13381 tttatttgat aatcaaagta gttccaatat tatatcaaaa aaattgttgg ctggctaata
    13441 aagtgttagg ttttacatac catctaaaaa atccttgagt tttcctagtt gttcatcatt
    13501 ccacggcctt aactttggcg tactcaaatc gatttgactg gacatcttat ccaaatctga
    13561 ttttactgtc gctacacgct gttccaattc tctagccgag acacgtaaag caccctgaga
    13621 aaatatagtc cattgttcat ttaaatcact ggtagcaacg gagactaaat ttaacacaga
    13681 ttgattaagt tctgccgctg ctcgttcaat atagctatcc gcagtttcgt cctcctcagt
    13741 aaagataacg gatattttgt attggtcata acgttgacgt acaccaggaa catactgtgc
    13801 atcaaataca caaataatat caatgtgttc gaaatgtgcg taatgattta gctttctaag
    13861 taatacttcc cttgcctctt ctaaacgatt gcttttaaaa agctgtcgag tatctttcca
    13921 aaaagcaatc atattgtagc catcaacgag taagatagaa tgttttttca taggcggttt
    13981 ctaaatactt catacatcaa aatagcggct gcgacactgg catttaaact ctgaacatga
    14041 ccattcatgg gaattgtaat catttcatct acttgctttt tgatattatg agaaatacct
    14101 tttccttcat taccaataac tagggctaat ttacctttgg tattccactt atgcgatggt
    14161 gtcccattca tatcagtgcc aaatatccag aactccttat cttttaaagt atccaaagtt
    14221 tgactgagat tagttaccct tgcaatagga acatgttcaa cagctcctgt agatgtcttt
    14281 gaaaccactg gggtcactcc aacagaacga tgtttgggga tgataatacc tgttacattg
    14341 gttgcatcag ctgtgcgtag aatagaacct aggttgtgag ggtcggtcag accatcaagg
    14401 attaaaatga gaggattttc ttcattctct gcctttgtca ttatctctga taaatccgca
    14461 taggcaaatt ccgaaacttt tagaacgaat ccttggtgaa ctccaccatt tgtcatgtct
    14521 gataaggttt ttttaggagt ccaggaaatg gatacttttt tttcagtcgc tagtgctttg
    14581 actttatcaa cgttctttcc tcttaagtca tcttgcaagt ataatttatt accagtatta
    14641 gctcttaaac tctctgtcac agcatgtaag ccatagacaa ggtcacttga ttcttcttta
    14701 aattgtttat ctttcatagt catattatat catgaatgct atagcataaa gctttttata
    14761 gcaatatttt accaacaaat cctaatgtct ctataaaaaa gagaggatat agcctctctt
    14821 tttgtttatt tttcaatggt ttcaatacac cactgtatta gagtttccaa gcgctttatc
    14881 tgaccagtca tatccaagta ccccataaga gcctcaaaac ctgtagacat acgataggta
    14941 ataatatcag catttttagc ttttgtatga ctattcgcat tacgtcctcg tttataaatt
    15001 aactgctctt catcagttaa aatattttct tctaacatgg cattaattaa caatgcttgg
    15061 gcattagcag aaacatattg ggttgctttc cgatgtaact gattaggttt ggtaaaaccc
    15121 tgcataatta aatggcgtcg gatatacaaa gaataaactg catctccttc aaaagctaaa
    15181 gcaataccat taatcaaacg aacatcaatc acgagtccac ctcacgccat ccttagtatc
    15241 taacaattta attccttgtt ttgctaattc atctctaata cgatcagcag ttgcaaaatc
    15301 acgattagct cgtgcttctt gacgttgttc tattaaagat tcaatatcag catcaagaac
    15361 ttcctcttgg aatacaatac caaaaatttc taataattca gcaaaggttt ccttaactct
    15421 tgaagtatag tgtcctgaat taatccattt tgccatttca aagataacag taataccgtt
    15481 tgctgtatta aaatcatcat ccatcgctcc ttgaaatgct ttcacaaatt gctctaattc
    15541 ttcatcatta gcattctctt ggattggtaa gttgaaagta ttttttaaat atttaaggtt
    15601 aacttccgca tcatgaacgg ccttttcagt gaaattcacg ggtttacgat actgttgtgt
    15661 cgctaagaaa aaacgtatta cttgtccgtc aacgctcttc aacatatcgt gaacagtaat
    15721 aaagttacca agagatttag acattttctc gttatcaacg ttaacaaaac cattatgcat
    15781 ccagtaattt gcaaaggtct tacctgtttt agcttcagat tgtgcaatct cattcgtgtg
    15841 atgtggaaat tctaaatcag ctcctccacc atggatatca atagtatcac caaagatctc
    15901 agttgccata acagaacatt caatatgcca ccctgggcgc cctttccccc aaggactttc
    15961 ccatgagact tccccagatt tagctgattt ccaaagtgca aaatctaaag gattctcttt
    16021 aatctctcct tcaccatcaa cacgaccact tgcaccaatc tctaaatctt ccaaagtttt
    16081 attggctaat tttgcataat gatgtgattt acttactcta aaataaacat ctccattagc
    16141 ttcataggca aattccttat caactaaaac tttaacaaaa tcaataatct catccatata
    16201 gtcaataact cgaggatttt ttgtagcagg tttgacacct aatgcagcaa catcttccat
    16261 aaatgctgaa ataaacttat ctgaaaagga ttttgtatcc atacctgctt cagctgcacc
    16321 ttttataatt ttatcatcaa catcagtaaa gttagaaata taattgactt ggtaaccaca
    16381 atactcaaaa taacgacgaa tagtatcaaa agctactaca ctgcgggcat tcccgatgtg
    16441 tatatagtta taaactgttg gcccacagac gtacatgttg actttacctt cattgagagg
    16501 tataaaatct tgtaaactgc gagtcatagt gtcgtaaatt tttatcatgg tttttccttt
    16561 ttctgttttt gataagcttt atacaaacga acaacataca ttaaaacaaa agcagcccaa
    16621 ataattccta agagagggtt accttgtgca aactttaaat agcttaaaca agctgctgct
    16681 agtataccta agattgataa tatttgagtt gttacataat ccttcacaaa tacctcctaa
    16741 agtttggacg aataataact ttcttctcga tcatgttcaa tacttcggat ttgaagatca
    16801 tccttttgac catggacacg gacaacttta gctggaacac caactactgt tacgtctgct
    16861 ggcacatctg caaggacaac tgctgcagca ccgactttag cattttctcc aacctcaatt
    16921 ggaccaatga tttgagaatg tgctgaaatt agcgctcctt tacgtatggt tgggtgtcgt
    16981 ttgcctttgt ctttacctgt gccacccaat gtaaccccat ggtatagcat agcacctttc
    17041 tctacaatgg cagtttctcc aatgaccaat cctgaaccgt ggtcaataaa aaccccttcc
    17101 gaaatcgtcg ctccgggatg aatttcaatt tgtgtccaga aacgccaaaa ttgactgtgc
    17161 atacgtgcta acaatttaaa attatgattc cataaaaaat gagatagcct atgagctgca
    17221 agagctttta ttcctggata agttaaaata acttctagag aactacgagc agctggatct
    17281 tgttctttga caatagctat actttctttc caccacccca tagttatcct caatcgtttt
    17341 ctacatcaga ccaatcaact aaaacttgtt gttcatcaaa tggattgatg gctactccaa
    17401 aggtatcatc aatctcattt tctccattac gcggtttata aatatcgtca atcgtccagg
    17461 ctaaaattac tccttgtgct ttcctaaaag caccaccaaa atcattatgg ttataccatt
    17521 tttcaaaact taaaaggttt gagaatactg gaacataact ttttccttcg ggggtagaca
    17581 ttgtagggaa aagacggtca aaactacctt cttcacgtgg atggacaaag gcaggtacca
    17641 agtaggtttt atccatgata tcggcaacaa tgttatcttc atttaacaac tgatttaaga
    17701 ttgtggtgta attattcata aattgcacca tatcttcaca gtagaaaata gtcgagttcc
    17761 caaaatcgcc ttcttttttt aaattataga ccaatcctgc taagccatgt gaaatagctt
    17821 catcaagaac gtctaaacta cgacgactct cccaaaacat atctcgagca ctttcttgct
    17881 cctctttgaa atgattaagg tcagtaatat ccgtaaaaac aggaacaact tcttgaccat
    17941 ctacttgaat agcatagggt tccttgcttg cccaaaccgt agagtggtgc aaggcattca
    18001 caagacctat actatctaaa aaattatccg gtgcattgat aaaagcacgc aatctaatgt
    18061 ctaactcgtt tgtacttgtc atattaagat tcctttttag gattgtcagc ttttggagga
    18121 cgtggtaaca atgctttcat agaagcatca actcgccctt tatcatcaat tttgatgact
    18181 ttcacatcaa cttcttcacc aatttcaaga acatcagcta catttgctgt acgtgtccat
    18241 gcaatttctg aaatatgtac caatgcatct gttttatcaa agaggttgac aaaggcaccg
    18301 aatttctcga tacgaactac tttagcatgg taaacttctc ccacttttgc ttcacgtact
    18361 aaacttgcaa taatgtcttt cgtacgatca atagcagcct gatcactaga gaagatagat
    18421 acatttcctt cttcatcaat atcaatctta acacctgttt cagcgatgat tttatcgata
    18481 gtttcgccac ctttaccgat gacaacttta atcttatcaa catcgatttt tatcatatca
    18541 atcttaggtg ccgttggtgc taattgtgga cgtggctctg cgatagctcc atgaaggaca
    18601 tcaagaattt caaagcgtgc ttttttagct tgcgcaagag cttcttccaa aatttgaggg
    18661 gtaatacctt caattttaat atccatttga agtgctgtaa tcccttcacg tgtacccgca
    18721 actttaaagt ccatatcgcc aaaatggtct tctaatcctt gaatatcagt gaggactgta
    18781 tagtttgttc catctgatat gagccccatg gcaataccag caactggagc ttttataggc
    18841 acaccacctg ccataagagc aagcgttcca gcacatatag aagcctgaga tgatgaaccg
    18901 tttgattcta aaacttctgc aactagtcgg atagcatacg gaaactcctc caagcttgga
    18961 aggacttgtt ctaatgcacg ctctcctaaa gcaccgtgac caatttcacg acgccctgca
    19021 gctccataac gtcccgtctc accaactgaa tattgtggga agttatagtg gtgcataaag
    19081 cgtttcttat actctggtgt tagcccatca atgatttgtg cttcacccat aggtgctaac
    19141 gttaatactg ataatgcttg ggtttgtcct cgtgtaaaga gacctgatcc atgaacttgt
    19201 ggtaaaaaat caatctctgc gtctaatgga cgaatctcat ccacacgacg tccatcagga
    19261 cgaatcttat cttctgtaat gaggcgacga acttctgcat gttccatttg ttctaaaatt
    19321 tcagcaacat cacgcataat gcgatcatat tcttcatgtt cagcgtaacg tgcctcatat
    19381 tcacctataa ctacttcttt aactgcttct gtagctgctt ctctagcctt tttctcttca
    19441 acttgtactg cagcctgtaa agctatgtta tgagtagcaa taatttctgc ttgaagttct
    19501 ggatcaactt gaagaagttc tacctcggct ttttctttac caatagcagt aacaatctct
    19561 tcttgaaagg cgattaattc acagacagcc tcatgtcctt taagaagagc ctctagcatg
    19621 atttcttctg aaagttcttt cgctccagat tcaaccatgt taatcgcttc tttagtacct
    19681 gcaaccgtta attctaacgc agaagcctct tgttcttgtg ctgtcggatt aataataaaa
    19741 ttaccatcaa cataagcaac ttgaactcca gcaattggcc cattgaaagg aatatcggaa
    19801 atcgataatg ctaatgatga accaaacata gcagccattg gtgcgctagc attttcatca
    19861 aatgaaagaa cagtattgat gacctgtact tcgttacgaa atccctcagc aaacatcgga
    19921 cggattggtc tatcaataag acgtgctgtt aaggtagcat ccgtacttgg acgcccttca
    19981 cgtttgttaa agccacccgg aaatttccca gcagcataca ttttttcttc gtaattgact
    20041 tgaagtggga aaaaatcacc tgttgacatt ttcttagaca taactgctgc tgtgagtaca
    20101 gtagaatctc cataacgaac gacaactgat ccatttgctt gtttagccac ttggccagtt
    20161 tcaacaacta atttttttcc agcaaaaatc atttcaaaga cttgttttga catattattc
    20221 tccatatttt tttgtttacg cgtttcctac aaagttcaat ctagctgttc taaactcaac
    20281 tttgcataaa accacgtata agctttattc taacataatt ctataaaatt gagtagcgaa
    20341 aatccgtgga ggaagctgta ttatcattca ttaatgtaaa cgtaaaaagt ctgattatta
    20401 atactagcaa tcagaccttg ttacaatgat taaaataaat ttctagggtt aatcttagct
    20461 tgcttaataa ccctcttcat aggttaaaac actttcttac tgctggcctc ctcaaagtac
    20521 ttacgttcat agtgcgttta aaacaaaaaa cactagaaaa ttttctagtg tttttacttg
    20581 actctgttgt ctattaacga cgaagtccaa gtgattgaat caattcacgg taacggttaa
    20641 catctgtacg acgtaggtat gctaataagt tacgacggtg accaattttt ttcatcaatc
    20701 cacggtaagt agcgtggtct tttttgtgtt gtttgatgtg gtcgttaagg tggttgattt
    20761 cccaagtaag aactgctact tgaacttcaa ctgatcctgt atcaccttcg tgacgtgcat
    20821 attgagcaat gatttcattt tttttctctt ttgagattgc cataatatat tctcctttta
    20881 ttgcttcatc cgagtcttag gattggcact cctaaaacca agaaaaagtt ggtttgtctt
    20941 atagaccctc ttattttatc aaattagaat aaactttgtc aaaagttttt aatagaaata
    21001 tggataacca actgtctcta aaggaaaatc cttcatagac taaacgagac tttgcatctt
    21061 tttgccgaaa gaccttagca taagtagatg tcatactagt tggcccacac ataaatattg
    21121 ttggttgtcc ttcaaaaaca gactgactaa aatcaagtct tcctttgagg ctactattat
    21181 ttaagtgaag tttaaaatta ggattggctt tagcatagga ttctaacata tcttgataaa
    21241 tcaaattgtc ttgatttgaa aaggtataaa agaagtcaac tctcttagtc aaaatactat
    21301 tttctctaat aaatgaaatg aagggagtga tgccgatacc tcctgcaatc caaacctgtt
    21361 ctttcttgtc tttatcaaat aacatatgac cataagctct atctaaggct atttttgtcc
    21421 caacttttag ctgtttgtaa atggatttcg tataatctcc tgaagcttta acagttaaaa
    21481 atatcactct atcatgacca cctgatatcg agaaaggatg agctgctgat tcaaagccag
    21541 cttgataaat ttttaaaaaa gtaaattgtc catagtcata tcgataagga cgcttcattg
    21601 ctatttcaat ctcagttgta tcatggttaa gatgagtaac cttttgaacg taccctacac
    21661 gacgaaaacg cattctagaa tataggaaaa taatataaaa tccagatata accccaatga
    21721 cagcataacc taatacgatt aaactgagta aagtatttcc tagtatccta tctcctaaaa
    21781 tcataaaagt atgtaccaat cccaaaatat aagctaaata aacaaagcga tgaataaaac
    21841 gccaaatttc atactttaaa aaatttccga aataagctac aaaaacaata cttagaaaaa
    21901 gatataatcc cgcactgcca atagtctttg caaactcact tccatgtccg ccttgtccaa
    21961 gaccaatttt gtgcaataga agcaaaatca ttgaaaatac agccattacc ttgtggtaaa
    22021 aatacatatt ttcaatacct tgaaagtaac tttcaagtat cctaaatttg gttgataaga
    22081 ggaatgttag cgataaactt gtcaaagcta atcctggtac aacaaattca cttaatcctg
    22141 cattttgcca tgctactagt gtcaccacta tgctagataa aattaaaaca atacctttcc
    22201 aagctcgcat aacgaccacc cctttatttt tagtaaggat aaaataccac aagatactta
    22261 tacaaaactt aaaaatttca gaaaataaca aaaaggcaac tccaatgttg cctttttgtt
    22321 atgatttata aattgatttg caatgctgta ctatatcttt aacagcgagt ccatactcct
    22381 ctaataaata ttccatctga cctacctgac caaatcgctc atcaataccc atacgactaa
    22441 cagtagtgtc tttctccata ctaagccatt cgcataatgc acttccgata ccgccaatac
    22501 ggttatgatt ctcaatagtt acaatggatt gatctatcag taatggtttc agttcttctg
    22561 gaaggggctt aattttaaaa aggtcgataa ctgaggcctc aatccctagt tctttgaggt
    22621 agtcggctgc ttctatcgct ctagaaacca taataccact tgctacaaga gtaatatctt
    22681 ttccctggcg taattgtata aaaccctttg aaaaatcttc gcgtccctca taaactgcag
    22741 tgggggcctt acgaatagtt ctaatgtatt ttaggccatc aatacttaat gtttgtttta
    22801 aaattgcttc aaactgaata tcatcactaa cctcaaaaat agtcgctttg ggaattaacc
    22861 ttagtagacc taactcttca aaaggcatat gagttccgcc attcatttct gcgctaattc
    22921 ctgcatctga gccaataatc gtagctgata attgtgagta acctaacgat aagaatactt
    22981 gatcaaagac acgccttgac gcgaaaggac caaaagtatg aaggtatggc ttgtaacctt
    23041 taatggcgag ccctgctgca aggcctacca tctcagcctc cataatacct agattaatat
    23101 aacgttttcc aaattctgat gccagtgcat ttgttgacat tgaactcgac aaatcggctt
    23161 ctaaaacggt aatttgttta ttttcttgat tagcttgaag gaggaaatca cgatagacaa
    23221 gcctcatttc tttggttgaa catctcattc taccacctcc aaactttctc tcaagcttat
    23281 caacgctcgc tctaacattg ttttttgttg taaatctggt cgtaaatgat gattagaagc
    23341 taactcctcc aactctttca caccttgtcc cttgatgcta tctaaaacaa tacattttgg
    23401 tctgacacta tttgaatctt gaaaagtttt aatagctttg tcaattgcct caatgtcatc
    23461 tccttttaca cgtactgcat caaatccaaa agcttcaaat ttagcaacaa agtctccagg
    23521 attacaaata tcagctgtca aaccatctaa ttgtttttta ttatcatcta caaaaacaat
    23581 taaatggtgc agttgatgat gcgcagcaaa ttgtatagcc tcccaacatt gtccttcatt
    23641 taattcacca tcacctacaa tagtataagt ataatagctt gaattctcaa tcttttgagc
    23701 ataagcaatt cccgttgcaa tactaatgcc ttgacctaac gagcctgtcg ttacatctat
    23761 accaggagtt aaattgcggt caggatgcga aggtagtttg gtaccgtttg tattgagcga
    23821 atgaaggaat gttttatcaa aaaaaccttt taaatagagt gtactataca aggctggacc
    23881 agcatgtcct tttgataaga ccatataatc ccgatcactt tctttgaatt tttcaggatt
    23941 tatatccata atatcaccat acagaacagc caaagcctca acaattgata aactaccccc
    24001 atagtgaccg aatcctaaat ggtttagcgt ttctagagta tttaaacgaa tctccgttgc
    24061 aaatcgtctt agagatgaat atctttcttc tggtgatgtc attattaacc ttccttatgg
    24121 gttgattttc caacaaatga aatgccaaaa agtccaatta agataactac aataccaata
    24181 acaatagcta tcgcaccgcc gacatgatta agagcgccaa gaatgatacc ggtcaaacca
    24241 aaatcagcat ctgagaatgt tgaccctttg aaaccaagtc cacctagtac aggcatcaag
    24301 aaaatcggca agaaactgat gaggatacct tgaacaaatg caccaatagt cgctcctctc
    24361 acaccaccag aagcattacc gataacacct gccgttgccc cacagaagaa atgaggaacg
    24421 acccctggta aaatgacagt agtgccagtc acaatcatga ctatcatact aaccaatcca
    24481 ccaacaaaac ttgagataaa accaatcaat actgcattag gtgcataagg gtaaacaatt
    24541 ggacaatcca aggctggttt tgaatttgga accaattttt ctgagattcc cttaaaggct
    24601 ggtacaattt cacctaggat aaggcgtaca cctgctaaaa taacgaatac acctgccgca
    24661 aattgcccag ctagttggag ggcataaaca agaccattga caccgttact tatttctttt
    24721 gcaatataag cctcaccagc aaacactgca acgataaggt aaataattgc cattgagata
    24781 gtaatactta cggtactgtc acgtaaaaat gataaactct ttggaaattt aatatcttct
    24841 gttgattttg atttatctcc aacaattcca ccaataaatc cactcaacca atagcctaaa
    24901 gaaccaaagt gcccaagtgc caccttatca ttacctgtca gttgaatcat gtatttttgc
    24961 acaaatgcag gagatacgct cataataatc cctaacgcta gacctccaaa aaggatgagc
    25021 gagaacgaag taaagcctgc tacggcaaaa ataacagcaa tcatacaagc catatacaat
    25081 gtatgatgtc ctgtcagaaa aatatattta aattttgtaa aacgagcaat taaaatatta
    25141 aaaatcattc ccgctaacat aatcaaagca gttgctgagc catacttcgt aagagctact
    25201 gctacaatgg cttcattatt aggaactacc cccaccaaat gaaaagcatg ctcaaacatt
    25261 tttccaaatg gattcaagga attttggact atccctgcac ctccagaaac cactaagaag
    25321 ccaacaaatg tttttattcc accttttaca atatcaggaa cacctttttt ctgcagtact
    25381 aaaccgataa tggctatcaa ggcgactaaa atagctggcg tgctggcaat attaactaaa
    25441 aaatccaata gacctttcat ggcatcaacc tcctagatta agcctttttc ttggcaaata
    25501 cctgtcacta attctctcaa ttcatccata tcaatgatac tgtttaaaat acgaacatcc
    25561 cccaggtgac cagctgaatc ctccaaatca cgaccaacaa tccaaacatc tgctgcgctt
    25621 ggatctgctc cacctaaatc ataatgttca acttcaacat cagaaacccc caaatccttc
    25681 aaaatagact caatattcat ttgtaccata aaacttgaac ctagacctga tccacatgct
    25741 gttccaattc ttaacatctt taatcctcct gttttataat catttcaata tcttgatacc
    25801 cacgtgaagc taacaaagct ttaacattat tattatcacg aagtattgtt gtcaaatgcg
    25861 atagagcttt caaatgcgtt tcattatcaa tagctgctat gcataataag agataaattt
    25921 cctgtttggg gtcatccaat aaataaatag gctgttccaa aacaagcatt gacataccga
    25981 cagagtttac cccatcttct ggtctggcat gtggaatagc aataccttta ccaagattga
    26041 taaaaggacc aaactcttcg accttttcaa tcatagcttc tgggtaactt tccgtaattt
    26101 ttccagaagc taacaagggt tttgccgcta aacgaattgc ctctttccaa ttcatttgtt
    26161 cagaacttgt ctgataagtt tcctctgtta taagttggtg caacaagggc ctcacatcct
    26221 ttctaaatat atcttgtttt accattgtcc tcaaagccaa ttttaattcc tcttcacaat
    26281 gaacatgagc gtattttttt atagttgcta ttaattgatc aagttggaaa tcgtcaaggc
    26341 aagctttggg aaaatctgaa ataaccagtt ctttgagctg ttgtacttgc tctgctgtca
    26401 tcatcagcga aactagataa ttaggttttt ttgtttctac aaatatagta gaaaagacca
    26461 tatcgtaagt ttgattgtca atcagtttca gctgctcaat tttagagaca cgatgaaaat
    26521 gaatttgagg aaataatcca cgcaattttt ccttaatcac taaggatgaa ctaacgccgt
    26581 ttggacagag tatcaaagct ttataagaca ttgattgtgt accacctgat tgtctaagat
    26641 agcctccaaa gtgaatcacg aagtaagaaa tttcactatc gggtattaat cctacttgct
    26701 cttccaaagg acgtagtgcc tttttaacca atagaaataa atcactgtaa tgctctttaa
    26761 tattttgtgt ataaccacta tcgccagtta aaccatattt caatcggaaa taagctggaa
    26821 taatatgacg cttcagacct tgtaataact cttctttatt ggaaaaatta agaagtgaaa
    26881 cagtttccat ctcatcaacg attgcttttg ccaatgcttc aaagaaatca tcatctttag
    26941 ttccctcacc ttcaaaacag ccagataaga tcaaggtaat ataatctttt tctttctgat
    27001 ctaatggttt agataaggaa atatcctgac ttaaattagt cacaataata gtcgttaact
    27061 cttttaattg ttcagaaaca ataggagaac cctgcaaaac acgatctact gacctttgat
    27121 aacgacacag tattaaaatg ataaaatata aagatttctc aagcctatct tgtattggag
    27181 aaagttgaaa agattcatag aaatattcta ccgtcttttc taacttctca tatgagagag
    27241 cgaatttcca agaagacaag atataattca aagaccaaaa accaatggga gattctagta
    27301 gttgagttat catctgaaaa gcaacttgat gcttatccat ctcatctcct actagactat
    27361 atccctttgc tcttgtataa gtgagtgaga tacctctttt tgctaattta gaacgtagca
    27421 tcttgatatc tgataaagta gtattcttac ttactttcaa aaaatcttga taatgcacat
    27481 tggagacaaa ctctcttcgg caaaagctat aaaggtaaat cagacatacc ctttcttcct
    27541 gggttaggta aaattgctca gcttctaata aggagacaat tttatcatgc tctttttcat
    27601 ctcctaataa ctgataacgt ccagcagagt gagcaatagc tggtaggtga ttttttgcta
    27661 aaaaactatt gagatagtta atttgctcta tgataacttt tattgaaaac tttgtttgca
    27721 ctgttaactc atacaaggaa agatttttcc gagctaaaaa taccaacatt atcacaacag
    27781 tcttattatc aaccatttgt tttcctttct atttttattt tatagtaacc gtttacattt
    27841 ttctaggtca tttgagccca atctggcact actatcaatt gaatgaattt gggctattgt
    27901 tatagagtaa agaaaaaaga aagaaattgc aaaatctaga gcaagattcc tttccacaag
    27961 cagcacttta aaaaccttta cttaattgaa taagtaaagg tttttaaaat gttatgacac
    28021 taagatagct aaagtttgat aaggttgcaa agtaaccgtt tcatcaagag taacatcctt
    28081 ataatttgat agaagaactt gtccttggag ataattctcc ggtaatttta tcttaacttt
    28141 ctcagcaaag aaattattta aaacaagaag tttttcctta tcaagatggc gttcaaaagc
    28201 atatactttt tcattatctt tgaaagctgc tttataattc ccgtcagcta ttattggcag
    28261 ttgtttacgt agccttatca actcttgata gaaagtaaat atcagccctg ttttctcctt
    28321 tgcgacattg atttctttat atgattttcc tactttcaac catggagcgc cttcagaaaa
    28381 acctgcattc gtactatcat cccattgcat tggtactcga gaattgtctc gagatttcgc
    28441 tcgaataatg ctaaatgcct cttcttgtga cttgccttca tcaagcatta tttggtaggc
    28501 attcaaactc tcaatatcca cataatcatc catagaactg taatcgggat caagcattcc
    28561 gatttcttca cccatatata tataaggagt accacgcgat aagtggatag atgctgctaa
    28621 catggttgct ccttcatttc gaaaacgttt cacgtctaca aaacgattta atgctcgcgg
    28681 ttggtcatga ttattataga ataaagcatt ccaaccattt ccttcgctca tcccttcgcc
    28741 ccaactatgg aaaaggtcac gtaatgcagg aaaatcaaaa gccataatgg tccacttttg
    28801 cccatcttta taatctactt tgaggtggtg aaaattaaaa gccatggata actcttcacg
    28861 ttctggtgca gtgtaaagaa tgcaatttgc aattgtcgtt gaagacattt caccaactgt
    28921 catgaaggaa tcatcttgtc caaaactagc attattcaac atcttgagat aatcatgtgt
    28981 tattggacga tcagtataag ctggttttcc atcattaatt gggcagttct caagtatctc
    29041 atctttccca attaaattaa tcacatcaaa tcgaaaacct ttaacccctt tatcacgcca
    29101 aaaattgaca actttaaaaa gttccttgcg aacatcggca ttgcgccaat taaggtcagc
    29161 ttgagtgata tcaaaaagat gaagataata cttccctgta tcaccaaatg gtgcccaagc
    29221 atttccacca aatttagata cccaatctgt tggattatca cgtaagataa agaaatcttg
    29281 atagtagcga tctcctgcaa gtgctttttt aaaccactca tgttctattg aacaatgatt
    29341 aagcaccata tcaagcataa aatctatacg gtattgacgt cccacctcaa tcatttcttc
    29401 aaaatcgtcc atcgttccaa aatcagggtt aatagctgta taatcagaga tatcatatcc
    29461 attatctcgt tgtggacttg gataaaatgg gttgagccaa accatatcaa taccaagttc
    29521 tgctaaatag ggtaattttt cgataatacc acgaagatca ccaaccccat taccagttgt
    29581 gtctttgtat gattttggat aaatttgata gacaacttta cgtttatcaa tcgtcatatt
    29641 ctactccttt cccaaaatgc tgctgtattt agcagtctaa ctataacatc aactatcatc
    29701 aaaaatataa catttttaca gtgatatttg ttcctatgag ttttttctta tgttcaaaat
    29761 aattcaagga gctaatgtat taagcccctc aaacaattcc tcaaattacc tctcatttta
    29821 taagagtgct cttttttaaa aagaatacct aattactttt aaattttctt ggcaaccatt
    29881 aatgcttgtc cacgttttat ctgtttgggt aaatctatca attcttctgg tcggaattct
    29941 tgttgattag taatcagaat aggagtttcc acaatgtatc cttcttcttt tatcattgga
    30001 atatcgaaag taatgagttt atctcctact tttacagtgt caccttgagc aacatgagag
    30061 gtaaaccctt ttccttctaa attaacagta tccataccaa tatgaataag aaactcaacc
    30121 ccctctgatg ttaacaatcc aatagcatgt ttcgtaggga aaagaactga aactgtcgca
    30181 tcgaccggtg aaactagttc tccatcactt ggatcgataa ccactccctt ccccataatt
    30241 ccttgtgaaa agacaggatc actagcttga tccagtggtt tagctaaacc tgataaaggg
    30301 cttactacac taagctttgt acctgatacg acagcttttt cttttgctga ttttgtttct
    30361 gttgttgaag caatgacagc atcaggtact aatttttcct cttcagtttt tgtcaaaata
    30421 cctgattttt taaagaatag tgtcaaaaat aaaggaataa agatagccac tgccatacag
    30481 ataaagaaat atcccatata tttaacgtta attgacagga agcctggaag accgcctaca
    30541 ccaatagagt tagcttggac attaaaagtt gtcgccaaaa gacctgccac acttgaacca
    30601 atcataccag caacaaatgg ataaatgtat ttaacgttta caccaaaaag cgccggctct
    30661 gtaacaccaa ggtaggctga gatagcagct ggaagtgata tttgagcctc tttttcatca
    30721 tgacgatgca taaaataata agctagtaca gctgaaccct gagcaatatt tgaaagagca
    30781 atcatcggcc aaaggccagt tgtatgtgtt tttgtatctg ctatgagttg agtatcgata
    30841 gcatttgtca tgtggtgcaa accagtaata acaaatgggg catacagggc accaaaaata
    30901 gcaccaaaga gccattttac aggaccagtc aatccaataa gaacgatagc agaaatccat
    30961 ttacctagtg tccaaccaat aggtccaagg acagtatgag ccaagataat agctggaact
    31021 aaggatagaa atggcacaaa aatcattgaa acaacttcag gaatatgttt tctccagaag
    31081 atttctaagt aagacaggct caaaccagct aacagagctg gaataacttg agcttgataa
    31141 ccaatttttt gtactgtaaa gtaaccaaaa ttccaagacc aatttttagc aatgtctgct
    31201 gctgaagtac tagctacaga ataagcatta agtaattgtg gcgacactag acagatacct
    31261 agaacaatac cgaggatttg agtagtcccc atcttacgag tcacagacca tacaatacca
    31321 actggtaaga aatgaaatat agcttcacct ggtaaccata aaaaagagtc tactccgctc
    31381 caaaacgttg aaacatctac tagagtattc caaattggat gcccagaaga gtctacttgc
    31441 ctgacaccat caacaacttt ttgacctaaa aattcaaatg gaacggcatc taaaatatta
    31501 cgaaaaccaa ggatcagacc accaacaata atggctggaa taatcggtgt aaatatttca
    31561 gccagcattg tcaacacacg ctgtaaaggg ttttgatttt tttgggcagc cgattttgcc
    31621 gcctctttag acacaccctc aataccagaa acggctacaa aagcattata aaaaataggg
    31681 acatcattac caataatgac ttgaaattgc ccagcattgg taaaggtacc cttgactgat
    31741 ggtaactcct caattacctt taccttagct ttactactgt cattcaaaac aaatcgcatg
    31801 cgtgtagcac aatgtgtcac tgcactaata ttttctttac ccccaattgc ctctaacaag
    31861 gctttggcat catgtttaaa ttgttccatc tgtttatcct tatctattta acggagagtc
    31921 tgtgactcca aggcagaaac ttaaataaga ctagcttaca acataattgt aaacgattac
    31981 atatccctat gcaaatcctt agcttatctt tttaaaaaat ctataaatta taaaataaat
    32041 tagtcaataa tacccaaaac attagaattt ctcaccaacg ttaccaaaat gttagagtca
    32101 tgagtagatc agtcaccctc aatactctct taataaaaaa cacctactaa ttgatatagt
    32161 aggtgagttt tttaatctcc attgaaagat acgatacgat tctcttcaat catatagcat
    32221 tggttacaaa gtttctctaa catagcgaaa tcatgactaa taacgatgag acctaaattt
    32281 ctatccttta caatctccaa catgcttttc catacactag cttgtgtaat gctatccaac
    32341 atagtagtca tttcatctgc aataaggtat ttagtctctg gatgtaatga acgcacaatc
    32401 gaaaagcgtt gtaattctcc tcccgagagt tcactaggtc gacgatttag ccatttttct
    32461 tgaattccaa aagcatctag taaatctcga cttgggtaat aggcttcttc caaacttttt
    32521 ttcataggcc ataaaggatt catcgttttt tcaggatgtt gttgaataag ttgtacaggt
    32581 ctaaatgctt tgctaggcaa attactgcca tcaactaata cttcaccaga tttaggatgt
    32641 aaaaaacctg caagcaccct ggataaacta gtttttccac aaccactttg tccaaatata
    32701 cctaaaactt gaccaggtgc tacctctaaa ttaatctcct taaaaagcca ttgatctttt
    32761 ttatgataaa agccaagctt tttagcctct aaggtcatga gtaactcctt tcaaaaattc
    32821 ttgctgtggg agagagcgcc ataaacttct agcaaattct gtttgtaact gctctccatt
    32881 tccgctaaag aaactagctg gagctgtttc aatagctttt ccctctttaa aaatagtaat
    32941 acgatcagca atttgactag ctgctacaat atcatgagtg ataaatataa cgcttattcc
    33001 tttatctgca aaggagcgta gttggtctaa aaccatttgc agagcatctg gatgtaatcc
    33061 aggggtgggc tcatccgcaa taatcaaaga aaccttatca ctaatacacg ttgtaaacaa
    33121 aacacgtcgg agcattccgc cagaaagttg gaaaggatac aagtcaccat cactttcttt
    33181 taaaccaaac tgttgaaaca atccttcttg agtagccttt gaattttctg agatacctaa
    33241 gcgcacctga tgtttgactt tcgtagatgg atctaaataa ttaactgatt gtggaatcaa
    33301 cgtaatatct tttcctcgca actgtttaat gcgtttagaa tttagtgatt gaccacgata
    33361 aatcatatct cctgttacag atgcattttt aggaagaata tccataatag catgtgctaa
    33421 taaactcttc cctgaaccac tagcacctat aatagctaat aactcacctt ttttaatttc
    33481 taaattcagc gcttgtatcg gtgttgattg aaatggtttt aaaaatcttc cgtattgagt
    33541 gaaggtgatg gagaggtctt taatgcttaa taatgtttct gtcatatctc tacctcctaa
    33601 aaatgatcag tttgagggta aaagagtttc tttaaagatt ctccgatagt atcaaatgca
    33661 ttgacaacca aaataagata aagtcctgga aagataacca accaccaatt tccaagagag
    33721 atatgcttag ctgcctctga cagaatgata ccaaccgaag gttgttcggc agagagccca
    33781 aatcctaaga aagtcattga tgcttcatgt aggatgacat gtggaaataa gaggataaaa
    33841 ccaatgaaaa tttgagaagc aatcaaaggc aggatatgat gcctcacaat ataataaggc
    33901 gtttttccca tacttttaga aagttggaca aattctttat tctttagatg atagacttca
    33961 ttgcggataa gccttgctaa agaaggccaa tgtgtaacag ccgttgcaat gatgacccct
    34021 tgagcacctt tcccaacaac aaaagaaatg agaatcataa aaatcaaatg aggcatacca
    34081 ataaacaaat caactaacca tgctattatt ttatcaataa tgctatttcc taaacctgca
    34141 agcactccaa aaactgtcgc cagaatgacc cccataaggg cacctaataa gccgacttgt
    34201 agagagaaat aaagtccttt aatcgttctg acaaacatat ccctacctaa accatctgtc
    34261 ccaaaaagat ggtttaatga aggagcaagg ttccgtaaag ctgcattggt ttgcaaggga
    34321 gtactatgga aataaagatt taaggctaga atactaagaa tgagagaaat ggcgataccc
    34381 agttgccata aaacaatagt tcgacgtctc aatatcatac ttttctcctt aactgtggat
    34441 tgattatgct gtttaaaata tccgcaataa gattgcccgc aaaaacaaat aatgtcccta
    34501 tcatcacaat agctagaagt agcggtgtat cacttttaag tcctgcttca gttagggtag
    34561 accctagtcc tggatatgag aaaacttgct cagcaagaac ggatcctcca aacaattctc
    34621 caaaatagga aaaatgcagt gtaatagctg gtacgatagc atttctaaga caatgatttt
    34681 taaaaatttg ccattccgtt tccccacgcg ctctggcaaa taagacatat tcactagaaa
    34741 gtaccgacat cattttagtt ctagtatgaa gagttacatt ggcaatgcct agaatactta
    34801 gcgtgaaaac aggtaaaata aggtgcttaa tacgatcagc taacgtaata tcttgactca
    34861 aagtacctat cggggaagaa ataccaatcg ggaaccaccc cagctggaca gaaaagatta
    34921 ataaaaaaat gagcccaatc caaaaagttg gtactgatat ctgaaggtaa gaaaaccacc
    34981 ttacaattcg atcaagcaat ttcccttgat ggaaagctga taaagttcct aagataaatc
    35041 caataagacc cgataagacc caggagagtc ccataagtat gaaagaggca cctgcccgtg
    35101 atctaataat atcaataaca ggttgccgat aaactagtga agtccctaaa tgtccctgta
    35161 taaaattttt taaccaaata aaatactgga ccggagctgg cttatccaaa ccatagtggt
    35221 gagcaatcgc tttgtactga acaggggtta gtgacgtgtc ataattgaca cttgccatga
    35281 ctggatcaac cggtgattgt tttagtaaaa taaaggtcaa aattgatact ccaaaaacga
    35341 gtgacaaaca cctaagtatc ttccagataa ctacagtggt aatatgtttc acagataaat
    35401 ctccttaatt gttaaaaaag gagtcgagga aaccattcac accataaggt gtacattgtt
    35461 ttctctgact ccaaacttaa agtagcctac attaactaaa ttagttactt agcagattca
    35521 tcccaagtcc attcagcaat gttagtcaat aatgaccaat catgaccatg actatggaca
    35581 ccttgtttac ctacattgat acgtttatca ccaatataag tatggttaag actcaccaac
    35641 catacatttg gtaaatctcc aagagtggaa gcacctgttt tgccatccca ctgagctaac
    35701 ttccaatatt tgttagcttt atcaaggtca ggagatgtca ttgctttgtc aaggtactta
    35761 gtcacagtag gattgttata aaaagtaata ttggtccaac ctttaccagc taaacttggg
    35821 taatgtgatt cataaaattg ctgcgcatga tgacgtcctc ccgcataaag taaggctgaa
    35881 tcatgtgact tagttgccat ttcatcccag ttactagcct tgagtttaat agtaatacct
    35941 aaggctttag cttgctccgc tacttcaacg gctaagtttg ctcgtaattg atcattagta
    36001 gggtagtaaa ggtcaaattc cgctttaaga tttccttttt tacggctacc gtctgcttgt
    36061 tctttccatc cagcttttgt caaaagttgc ttagctttag ctactttatt atctttaatt
    36121 gctgtttttg gattccagaa tggtgttcta tcaataattg aataagctgg tttaccataa
    36181 ccatttaaaa cagtatccag aactttttgt ctatttaaac caatagtcaa ggcttttctg
    36241 attgctggat cactagtgac atcatttcct actggataac cgtctggtga atttttcaca
    36301 acacctttct tcacataagg caatgacagt ccgcgaacgt cgtttgaagc aatatctaga
    36361 agacgtgttc ctttgacttt cttgctagca agctctggcg ttgcgtaaat catgtcaaca
    36421 tcaccagatt ctaaagcagc tagtgctgta ttttcatcaa gtaataccca agtccacttt
    36481 ttaaaataag gctttttacc gtgccaatag ggatttcgaa cgaagattgc ttgttctcca
    36541 gccttatatt ctttcaccat gtaaggtcct gaaccgatag gattgctctt atacttatca
    36601 ttgtaatgtt ttttagggac gattgggatt tctgtcaact gtgctgtaaa tgtcgaatgc
    36661 gcctcagtca aatgaatatt tacctggttt ttcccaacta cttcaacatt cttaataaag
    36721 gtcaaatccc aagcttttcc atctgctttc aacatatcat aagtaaactt aacatcgtca
    36781 gcagtaacag gctcaccatt ggagaattta aaatcatcat ttaagtcgaa cgaccacgtt
    36841 aagctatcct tagagatttt atatttttta gcaagctctc cttttatatc tagttcagga
    36901 gaacgtttca atagggtgct atgagtaata ttaccttcat tatggattcc ataacggtcc
    36961 tttggatcga attcatgagg aagctttgcc cccatagaaa caaccaattc atctttgggt
    37021 cgttgttttc gcgttcgctc cttggtttgt gaattttgat tttgacaagc tactaaaaag
    37081 atactcgcca gaaacaaagc aacaaaagag atgtacttca aatatttagt cactattaaa
    37141 cctccttttt taattgataa ataatatttt tgacaacttt tttcattata acaagaaaaa
    37201 agtaggagaa caattactta actggttaat tagaaaattt gttattttat cattaataac
    37261 ttaaagttag gtaaaacaca attctagaca ctagctcata aaccatgtta atccaatgta
    37321 ttttaataga catcataaaa aggctgaata aattcagcct taaccacgct attagttata
    37381 gcaaaccaat caaatgacta aagataggaa taaccaaatc aactaccaaa ccgataacca
    37441 ccacagctat agctgccatc gcaccttgca gttctcccaa ttccaatgca aaggctgctc
    37501 cgactgtatg accagaggtc cctagaccta atcctgcacc aatcttacta tcattcaaat
    37561 ggaaaaatga cactaatttt ttacctaatg cataaataat aactgcattt aagatacacg
    37621 ccattgctgt cacagctgtg ttccctccaa ttgctgctgt tattggaaga gcaattgctg
    37681 ttgttgctgc ttgtggcaac attgaagcaa ttccgacttg actaagccca accattttag
    37741 aaatagcgac gataagtata agggaaacaa tcataccgat tactaaactg ctgagaattt
    37801 ctacccaata ttttttaaca acgtcatttt tcttatagag aggaactgca aaagcaattg
    37861 tcgctggatt taaaaaccaa aatattaaat caccacctgg tttataagct tgtgtataaa
    37921 atgtcttaac atcggttcct aaaccttttg acataagcca aagaatgaca ataccactaa
    37981 ccattgcaaa gaaaagaggc tgcaaaagga agaaaccttt actcttctta aataaatgtt
    38041 gtcctatcgt atagagtatt aaagaaaagc aaataccaaa gatgggtgtt tttaagagtt
    38101 ccatattatt tttcctctac tttcttacta tagtggttag gtaatatctt tttaaataaa
    38161 tctgtgacac tgagttctct gatagcaagt attaatcgtg caacatatgc taccactacc
    38221 aacataacaa cagttgagat tgtaataaca gcaactagtt gcaagccttc agcttttagg
    38281 atatcaagat tcgctgctac tgatattcct gaggggacaa acataaaacc aatcatgctt
    38341 atcatcaaag ccccaaaaga gtcaacccac tctaccttga taattttagc agttagcaat
    38401 acatacatta gcaccaaacc aataaccgtc gttgggattg gtagtgattt cggtaataac
    38461 attgaaatca tttgtgaaac taatagaata gctgcatata ttgacatttg aataaacatt
    38521 ggagcttctt ttgttttggt ttctttactg gtgtgtttca tagtcactac cccttccttt
    38581 tattacaatg ttttaacttg ttttattgat agctatattg tacttcaaaa caaataaaac
    38641 gttttcattt ttagtctcag ttaccaaaaa aagaagccga attgcaaatt caagcttcta
    38701 aacaacaaag ggaagttcgc aaaagcactt ttacatggtt aaatgctcat ttaggtcttt
    38761 taaatacgat cgacctactg gaaatttctc tccattagac ataattagta acaaagtatg
    38821 attaaaccaa ggttgaattt cctcaatcat ctctagatta atcactgtgt ttcgatgaat
    38881 ctggagaaaa tatggtgcta cagcgcgtga cttaaaccaa ttgagtgtct tccgaatagt
    38941 gtaagttttc tgaactgtac taacagttaa ttcgccatct tcaacactag ctgcaacaat
    39001 atcttgcatt ttaacaacaa cactgcgatc tgataatgtc aaggttaaca attcaacact
    39061 ggctttttta ggtattgctt gctctacatc tgaagcagtg gtgagttggc tcaatttctt
    39121 cacttttgat agagccatat caacgcgctg ttgttcaaac ggtttcatga tataatcaac
    39181 agcgttactt tcaaaagctt tcacagcgta gttatcataa gctgtcgcaa aaacaacaag
    39241 tgggggatgt gcaagttggc tcaactgatt agccaaagta aagccatttt cttcactaag
    39301 cgaaatatct aaaaaaatta aatcaatttg ctgtctaaat aagatttttt cggcttcact
    39361 gatatcctca gcctgaaaaa tctcagggtt gtcaacctcc tggctatgtt caactaaaaa
    39421 cgataattct tgtctggcaa acatttcatc atcaagaatt aaaattttca tgctcatcct
    39481 cccttattct attaggtatt cgataccaaa cttttgtacc attcttgtcg ctcgaaaaat
    39541 gaaggcaact tacactacca tataataaat tcagcctgtt atttagatta actagagcag
    39601 tacctgtacc cttactctct gcaactgttt cttgacctaa tttatcaatg atagtatctg
    39661 agattccttg tccattgtca ctaacagaaa cacaataata atgaccatct ggctttattt
    39721 gaaccaatat atggttgtcc gtcttacgtt ctttgaaagc atgtcgaact gcattctcta
    39781 ccagtacctg taaaccaaaa ggtggtaact tcattttttc tggtgcacta atatcataag
    39841 ataactgata tttatcaggg aaacgtaatt tttcaacatt catataagca tccacatgtg
    39901 atttttcttg ctcaagcgtt acctcacgat cctgaccacc ctgcaaactt gttctaaaaa
    39961 aagtacttaa ctgcatcagt gcataacgtg ctttatcaga atcaatacgg attaatgcac
    40021 taattgtgtt aatggcatta aagaagaaat gagggttgat ttgtgcttgt aaagccttta
    40081 tctctgccat actggctaac ttattttgtt cctctgttat ccccattgcc agttgtcctg
    40141 aaaatatttg cgctaaacca aggactaggt tttcctccac ctcagacatt gtcttatctc
    40201 ctgcaaagta catttttaag gcacccacag ttttatcatt tatttttaga ggaactacaa
    40261 tagcagaatt taactgacag ttgtgatctg gacaagaaat cgccgcttta tcttgcgcaa
    40321 ttcttggttc gccatcaaaa ataacacttt tagataagtc tgttttgacc ggttgtcctg
    40381 caatatggtg atcatggcca acaccaatat gagctaatac gtttgaccga tctgttaatc
    40441 ccacagcatc aaagttagta tgcctcttta taatttcgca aacgctccta gcagattgcg
    40501 gtgtcaaacc ttgtctaagg tagggcagag tctgtcgagt caattcaaga acatctctcg
    40561 tttgaactgc gcgtaactga ctttcatttg acaaataagt tttcaaaatc gcaaggaaaa
    40621 gtgtggaacc taaactattt aaaatcatca ttggaatgac aatcattttg acaagttccc
    40681 atcctgtaaa aatgccaaca aatagcatct ggatactttc ggcaataata ctaattaaaa
    40741 taacttggct tgttgaaggg tagagatggt tttccttaag cttatcacca atctttccgc
    40801 taacaatgcc gactagaact gaactgacaa tatagaaaga acctgaaaag cttccttgaa
    40861 aaaagcgatg aactcctcca ataaaaccaa caattgatcc aaccagaggt ccaccaacca
    40921 aacttgccgt tgtaataact aaagtccttg tattagcaag tgagtcagaa tgagaaatcg
    40981 ttgttagaaa agggcgctcg accaaacttc gatccccttt tatttcaatt cctgttatat
    41041 tagatataat aacaaacaag ccgaaaatga tgacaaggac taccgtttca cgtttagacc
    41101 gctcttcaat taactgtcta aaataactat tatttaccaa taaaaaggct aaaatcataa
    41161 taattcctag cctttggaat aacaacacca tcaacatatg cttatcctcc tcaagatacc
    41221 attttactcc ggaagataaa tatagatatc ttgataatct gaaataacct tgtctgccat
    41281 tgataaatct tgcggtggat aatcaggatt agcaaagcca aagcaataca ttcctgctgc
    41341 cttagcagct aaactcccat ttcttgtatc ttctataact atacaaactt tagggtctac
    41401 atctaaaagc tctgcagctc taagaaaaac atctggtgct ggtttagaag acgatacatc
    41461 ttctcctgta accatgtatt caaagcattc tgtcacaccc aactctttta gattacgctt
    41521 aatatctacc attggagatg aggacgcaac tgctaagcga tagccatgtt ggtgtaacca
    41581 atgaatcagt cgctgagctc ctttgatggg cctaacacca tcacgcgcca caatcgcttg
    41641 tcgacgtctg ttcatctcag caatatactc ttttactgtt ttcggtaagc caaactcctc
    41701 tttcatagct tgccacatga attcaaaagt agtccccatg tactggtatt gatacgagac
    41761 gtccgtatca ataccctcct cacgtaacat ttctgtctta ttatctaaaa acgtgtactc
    41821 ggaatcaaca ataacgccgt ccatatcaaa aataatgact ttttccatct taaacctgct
    41881 ttctatcccc tttattgtaa tcgtttgcct ttctattttc aataaaaaaa ggttgctttc
    41941 tgtgaaaagc aaccagtata tttagaaatg atattttgag agtgatttta tttaaaaggg
    42001 gagctcctct tcttccaata ctaaatctga caaatcacca gaaacgttat tttcacgcat
    42061 agcacgttgg gcacggcttt ctagcaactg aaatgatgat gctaatactt cagtgatata
    42121 gtgcgtttga ccatcctttt cgtacttgcg cgtacgcaat tcaccatcta ttgaaattaa
    42181 agagcccttt gtcccatagc tcgcaagggt ttccgctaga cgaccccaca taaccacatt
    42241 aataaaatct gcttcacgct caccattact tcctttaaag cgtctattaa cagcaacagt
    42301 tgcacgcgtc actgacttgt cagttggtgt ttttaccatc tcaggctttg ctgttagacg
    42361 cccaatcata ataactttat tatacattga ttacctcctc actattatta ttcgaaaata
    42421 aggaagaaaa atgctaaaat atatgaaatt gttctgggag aattaagatg acaaatgtaa
    42481 ctagttataa agaaatgctg gctaaacctt ggggaaaaat acaatatgaa ataacttttg
    42541 ctcaattaag ccatatcaaa aatcagaacg ttttagattt tggagctggt ttttgtctca
    42601 ccgaacaaca tctagcaaaa gaaaacaatg taactgctat tgaacccaat cctaaactgc
    42661 tttacgataa tcaaagtgac aatatttata aaatcctagg ttcctacgaa gcattaagag
    42721 atttgcctga tcaaagcttt gatactatta tttgtcataa tgttcttgaa tatatagaca
    42781 aacataatca tcctgcttat tttgacgaat tttctcgact tctaaaaccc aatggagaac
    42841 tatctctcat caaacataat ataaccggta aaatattaca atcagttatt tttagcaatg
    42901 atacctccac tgcgatggaa ctcttaacag gagaagcaaa ctttaaaagt gccagttttg
    42961 accagggaaa tatttatacc ttagaagaac taaaacaaaa tacaaattta ttagttgaac
    43021 gttatcaggg tattcgtacc ttctattcat tacaaccaaa ccattttaaa actgaaactg
    43081 gttggctaaa caaaatgctt gctatcgagc taagtgttgc tgacaaagct ccttataaag
    43141 atattgcttt tttgcaacac atcacactta aaaagtcatt gtaacttctt aagtttacaa
    43201 ctaaaaatca acttagctga agctaagttg atttttacta atttttccaa tgtttattgg
    43261 cgtcgaaaga ctctccaaca attacctgat cagataattc aatgatacct ctgacttgag
    43321 gagcatttgg aagagctaac tcgcgagcac tacataacat tccaaagcta tcttctccac
    43381 gaagttttcc tgggaaaata aggctaccat ttggcatcat agctcctgga agagctgcaa
    43441 ctgttttaag acctactgaa gcattaggtg ccccacaaac gatttggacc gtcttcccat
    43501 cgttgatttc tgcttgacag atatgtaaat ggtcactatc tggatgatca atcatttctt
    43561 taatttgcgc cacaacaaat gtaggttcaa tattattaac aagagagtct tcgaatcctt
    43621 ctttcagtag ttctgcatta aggattttaa tatcttgatc agataaagtt atttgacctg
    43681 ctccctcaat aacaattaaa cttgacactt caaagatatt ccaagcaact gtttctttac
    43741 tatcttgcag atagacgcgt gctacttgtc cgcgacgatc aacatctagc ttagctcctt
    43801 gactatcctt aacaataacc attaaggtat caccaacatg ctctctatta taagtaaaaa
    43861 tcatttattc tcctttaaat tttgctattt tctgtcttat ttatagttaa tttcagcgag
    43921 aaatttagta atttcagcct ttgttttgcg attcttatta accaaacgac ccagttcctg
    43981 accattttcc acaacaacga aactcggaat cccaaaaata ttccactgct gagctaattc
    44041 tatatagtca tcacgattaa cacgtacaaa gacaaaatct gagaaatctt tttcaattga
    44101 tggcattact ggatatataa attgacaatc tggacaccaa tctgctgtaa aaaagaaaac
    44161 aactttctta gtgctatcaa tataggctgc tatttcttca taagattctg gtaaaatcat
    44221 ttgctcacct ccacataatc aagaacacct tgacgataaa tgaattcgaa aattctgcca
    44281 tcttccaaga caagaccacc actggtcatt ttaatatcag attcaaattc attaaaataa
    44341 aatacctgaa tatcacccat ctgactaaaa aattctctta aggtctgagt gatttgctga
    44401 cgtttataat ctgttacttt tttagttagg gtataaccag cagctgccag taaacccaca
    44461 ccagcaaggc cagtcccaaa taagattttt ttatttttca taactgttat tataccatac
    44521 cccccggaat tagacacagg aattgatgga agaaatccac ataacagtta tgaaaaaaaa
    44581 caacttattc cttgtgg
//
LOCUS       DATRNK010000009        41951 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.009, whole genome shotgun sequence.
ACCESSION   DATRNK010000009 DATRNK010000000
VERSION     DATRNK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41951
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.009"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..1813
                     /locus_tag="VBC52_000515"
     CDS             <1..1813
                     /locus_tag="VBC52_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEO6882120.1"
                     /translation="GFIFNNKDNTDLSTYMVPEPNGFIEFDNFELYKINYEIINNCEL
                     NLEIIVIANVIVRQYIKGEMEIDTKTKFVSVYAEVELDSGIKNFRIYNTEFKSDQYKK
                     SRNLKLSKDWVPYIRKKDFDAIAEKFLRKYYPQALTQPTPVPVETIVSEMGLSIHQEK
                     LTIDNSVFGKMVFKDTDVEVIEDEQLVSEHFNKGSILVDKDVVFKRNVGSYNNTVIHE
                     CVHWELHKVFHEVKMVLDKDHSQVSSWTEENLADSSMWTSLDWMEWQANGIAPRILMP
                     KVQTRIKIRELFQTLTLVNPDISRSELVQEVVDNLATFFEVSRQAAKIRMIDLGFKEA
                     NGVYNYLDDRYMHNFAFELEAFDKGSSYTITSNDLCFEYCFNESFRQIIDRNKFIYVD
                     NHLCLKDKKFIYMTKDGPIMTDYAYEHMDECCLIFKVKSKNFTSISNETYYDYVLNRG
                     VTKESEIKADFVDILQNPSLMDQLPPLDMMKLGKKISELLKELPFEFSGTLRSHRKRK
                     NCTQPFLAKLVGITERTLRDYETLEDNLPRLELTLSFCFALKLRPELSDDMIKKAGHQ
                     LTISPPHQVYKMLLSTSYYKPLGEINSILQAAEMKTL"
     gene            2222..2791
                     /locus_tag="VBC52_000516"
     CDS             2222..2791
                     /locus_tag="VBC52_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882121.1"
                     /translation="MPINVNFFNYCQTQSSSTGVEPSKGEVLAPMFVQDYYMSSEYSG
                     LIRGTFRQCRIMSIPILTAFVPVAEEEYEQMVWWYNNSVNDYLKDFRKPKKNTLEVSS
                     WEVFTEKQDLAIADEGIELYLVMDQLDFLKAKLAATNFQAPEILDMLFEGYENKEIFE
                     YLGVKKSAGYKKVNNAQKEGLKLYKELNK"
     gene            complement(2830..4785)
                     /locus_tag="VBC52_000517"
     CDS             complement(2830..4785)
                     /locus_tag="VBC52_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEO6882122.1"
                     /translation="MLIKELSIDLETYCEVDLRKSGVYSYAEDDSFEILLLAVSVDNG
                     PVTVYDLTKENLPDQILQALVNDSIIKWAFNASFERICLSNWLKKHHPKLLSEGFLSP
                     NSWRCSIVWSAYLGLPLSLEGVGTVLKLKEQKLKEGGDVIRYFCLPYKPTKINGGRKR
                     NFPHHAPDKWAAFINYNKRDVEVELAIKNKLRNHPVPDFLWEEYHQDQNINDSGIGID
                     VDFVKAAITIDEESKSKIQEELKELTGLENPNSVLQMIGWLREHGVTTNSLDKKAVKE
                     LLKVVDAKTTKVLKLRQQAAKSSVSKYQAMVNCVCLDGRARGMFQFYGANRTGRWAGR
                     LVQLQNLPQNHLPDLKEARDLFKTGDLEATDLLYGTQDTLSQLIRTAFVPSDGKKFIV
                     CDFSAIEARVLSHLAGEKWRSMVFEQGKDIYCMSASQMFGVPVEKHGRNADLRQKGKI
                     AELACGYGGAVGALKAMGAIDMGLDEQELQPLVDSWRQANPNIVLFWWDVDKAVKTAI
                     KYQKQTETHGIQFKVRKGMLFITLPSGRKLAYVKPKMGENQFGGESVTYEGTGTAKRW
                     ERLESYGPKFVENIIQAISRDILAYSMKQLKDFRIVGHVHDEIIIECDQSQNLEQIAT
                     LMGKAPYWMPDINLRADGYECLFYQKD"
     gene            complement(4835..4999)
                     /locus_tag="VBC52_000518"
     CDS             complement(4835..4999)
                     /locus_tag="VBC52_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882123.1"
                     /translation="MFETIFFYSLIGIYLFFGLYLNYMTIRDDIRREKERKAEKKHHS
                     NNTTPLHRSR"
     gene            complement(5016..5579)
                     /locus_tag="VBC52_000519"
     CDS             complement(5016..5579)
                     /locus_tag="VBC52_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HEO6882124.1"
                     /translation="MTTKVQTTKVITGKNTRFSYLNANEPKSINGSTPKYSVSLIIPK
                     DDIETVDKIKAAIELAYKEGESKLKGNGKSVPALSILKTPLRDGDLERPDDEAYRNAY
                     FVNANSPHKPGVVDANRQEIIDTSELYSGIYGRASISFYAFNSNGNKGIACGLNNLQK
                     LRDGEPLGGRTRAEDDFATDDDDDFLN"
     gene            complement(5584..6705)
                     /locus_tag="VBC52_000520"
     CDS             complement(5584..6705)
                     /locus_tag="VBC52_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HEO6882125.1"
                     /translation="MTNHAVLSASASHRWLNCPPSVRLTEDMPDVTSEFALEGTDAHE
                     LCAYLVEKALGRKARDPTEDLSFYNEEMQNCAEEYRNYVMEQVEKAKDYSHDPTVLIE
                     QRLDFSKWVPEGFGTGDCLIVADGLLQVIDYKHGLGILVDADHNPQMMCYALGALEMF
                     DGIYDFDNVTMTIFQPRKNNISTFEMDKAELLEWAEDQLSPKAELAFKGEGELKSGKH
                     CQFCKIKNVCRKRAEDNLALAKMEFADPATLDYEDIAEILPKLDLLVSWANDVKAYAL
                     KEATEGHSIPGYKLVEGRSVRKFSDEAAVSQAVMDAGFDPYEKKLLTITAMTKLLGKK
                     TFNDLLGGLIVKQSGKPTLVPLDDSRQELNLATNEFKED"
     gene            complement(6698..7021)
                     /locus_tag="VBC52_000521"
     CDS             complement(6698..7021)
                     /locus_tag="VBC52_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882126.1"
                     /translation="MSKMKQLNELINEMEGTAKYYLRLVDEFKKILSTEEETTTTSKE
                     PKHKPQKELKLEDVRSVLATKAKDGYKNEVRALLNKYGAESLSALATEHYAAVLEEAG
                     GIGHD"
     gene            7418..9697
                     /locus_tag="VBC52_000522"
     CDS             7418..9697
                     /locus_tag="VBC52_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO6882127.1"
                     /translation="MQFTLSHSGQSGVQTTTVYPHQVTITDETSLKRIAQYDHVAGLF
                     SNNTRSNANFLKSDVLVMDIDNDHTENHDDWITEEYLKILFSDYHFALVTSRNHMVQK
                     GSKVARPKFHIYFQINEVSDKDAYAFLKEEMTNRYNFFDTNAKDAARFFFGNPNAQVL
                     WNDSWLTIDADLLDSSFDDEEDFDADFYQPPTGPITEGSRNSTMSVFAAKILKRLGVT
                     KEARDGFDEQALKCVPPLEKAELDTIWGSAVRFYNKTIKNSKDYKSPEEFQRGEMQPD
                     DYSDIGEAGLLAREFGDKIAFTRETDYLAYDGKHWVEDEQLAMRQIHQFLDMQLEVAV
                     TKLNNATQKLKQSGIPEMLIQQGGKKLENAIETPIQLVAYQDYKKAFEFYKFVMKYRD
                     YRNLSAIAKMAKHMVRMSVSDLDKNELLLNTPEATIDLSQGLSGIREHDSADYLTKMT
                     NASPSDKGDGLWQETLATFFCDDTDLINYVQEIVGMAAIGKVYQEHMIIAYGSGANGK
                     STFWNTIARVLGNYSGKLSAEALTMSVRRNVSPEMAELKGKRLIIASEMSEGMRLNTA
                     MVKQLCSTDEILAEKKYKAPFHFVPSHTLVLYTNHLPKVGANDDGIWRRLIVIPFNAK
                     ITGRSDIKNFADYLYNEAAPAIMSWIIEGAEKAIKANFKLILPQVVADSVSAYREAND
                     WMGQFLGDCCEIGDHLTEKSGELYSAYRAHCARINEYTRSTTDFYTALANAGFTKKRT
                     NKGVMVKGLQLKLEDDFLD"
     gene            10048..10329
                     /locus_tag="VBC52_000523"
     CDS             10048..10329
                     /locus_tag="VBC52_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEO6882128.1"
                     /translation="MREKIVEQKLVSEVKKRGGVCPKWVSPSFGGVPDRLVFLPKGKF
                     GMVEVKAPGGKPRLLQVTRHKMFDGLGFKVHVLDSVEKIGEVLDEIEFT"
     gene            10310..11686
                     /locus_tag="VBC52_000524"
     CDS             10310..11686
                     /locus_tag="VBC52_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006268649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6882129.1"
                     /translation="MKLSLHDYQEVTKDFIIRTPYAAVILDMGMGKTATTLSAINELM
                     FDRYEVSKVLVIAPLRVANTVWSDEIEQWEELRHLRYAKIVGTPKQRRAALEQDADIY
                     IVNRENLPWLVEQCSPYFKWDMVVIDELSSFKSWQSKRFKAFMVMRPYMKRVVGLTGT
                     PSSNGLMDLFAEFKVIDGGERLGRFIGEYRSRYFREGARNGQVVYNYLPMDYAECQIY
                     DKIDDITISMKAMDYLEMPELISTKKVVHLTDKEKSDYKQFKKDYVLADLENGEVTAA
                     NAASLSNKLLQMANGAVYSNDHQVVSLHDQKLDALEDIIEAANGEPVLVAYWFKHDVQ
                     RIEERLVKLKVKGTILKTEEDIREWNKGKISVGLLHPASAGHGLNLQKGGHHLVWFGL
                     IWSLELYQQTNARLWRQGQQADTVVIQHIVTQGTIDEEILKALESKDAQQSRLIEAVK
                     AQVGGMNG"
     gene            11679..12188
                     /locus_tag="VBC52_000525"
     CDS             11679..12188
                     /locus_tag="VBC52_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882130.1"
                     /translation="MDRIEYLMKNYSDVKLKLALVENQLLNFRPISEESVIQSLVYEK
                     PDMERVKTSQINSRSETIALSFREKLEKENKEYWDSLMECYHFLKTELEFFESMVNLI
                     PDDLKQFSKDLIFNEMSWDDISSHYEISRSTISYRKRKVHQQLKKCYGWMSRSIDLDE
                     SAFQIPLSN"
     gene            12368..12550
                     /locus_tag="VBC52_000526"
     CDS             12368..12550
                     /locus_tag="VBC52_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882131.1"
                     /translation="MTKRQQEKLNALLTAIAQEELLVETLETRKSDNLDFYDVSVWGV
                     KRALERAYEAGQQSVK"
     gene            12598..13635
                     /locus_tag="VBC52_000527"
     CDS             12598..13635
                     /locus_tag="VBC52_000527"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEO6882132.1"
                     /translation="MIFTSEQVSSGHPDKLCDQISDAIVTKCLKHDKNSRVAVETLIK
                     DNQVVVAGEVSTKHLFNLEGIVKKVLEPYGMTDVMVTNLLGVQSPDIAQGVDKGGAGD
                     QGIMFGYATDETPEFLPLPYVLATRVLEKLMNLGHPALVKDAKAQVTYDYENKCIETF
                     LVSIQHDEEVDLASVKRIVTQAMMSVAQRYRQNLDFRVLVNPTGRFVLGGSYADAGVT
                     GRKIVADTYGGFACHGGGAFSGKDPSKVDRSAAYMARKIAKDFVREGYAKRCEVQLAY
                     AIGVAEPVGVYVNIFGTSDYPLEQLVGVVRERYDLTPQGIIKELNLLDVDYTKTTCLG
                     HFTKPYLPWEQ"
     gene            13637..14002
                     /locus_tag="VBC52_000528"
     CDS             13637..14002
                     /locus_tag="VBC52_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEO6882133.1"
                     /translation="MPRRPSTPCKQHGCPNLVTYGNKYCDDHKANYSLDAKSTKAKGY
                     DTRWNKARLRYLKFHPLCVYCQRKGRLTKATVVDHITPHRGDQDLFWNPSNWQALCKS
                     CHDRKTKTTDRYVEYTYRL"
     gene            14291..14746
                     /locus_tag="VBC52_000529"
     CDS             14291..14746
                     /locus_tag="VBC52_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6882134.1"
                     /translation="MNDNQRRGIWKLRRDGFGYGAIAQMLNMSLGSVKQYCRRHPKLK
                     GMGQFVKYQLDEGERSYCKNCMKKLHHAVQGRPKKFCSNRCRAIWWRNHQSQHDKTKT
                     AYDELTCQNCGRSFLSYANPTRKFCGHPCYVEHRFRKGVVHDNSTQYGN"
     gene            14718..15974
                     /locus_tag="VBC52_000530"
     CDS             14718..15974
                     /locus_tag="VBC52_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA modification methylase"
                     /protein_id="HEO6882135.1"
                     /translation="MTTQPNMEIKELPLSDLKPASYNPRKKLKKGDKEYEKIKQSLLK
                     FGYVDPIIVNDDLTVIGGHQRLTVLKDLKYETAKCVIVSLSKEDEKALNIALNKITGQ
                     WDDQLLADLLLDLQESDFNLDLTGFEPPEIDDILSNIHDKDLSEDDFDVDEELKKPTV
                     ARRGDIWQLGKHRVICGDSTKSENYEQLLGDKKANLIVTDPPYNVDVEETAGKILNDN
                     MSDRDFYQFLFDMFTQVESHMEADASIYVFHANTEGLNFRKAFKDAGFYLSGSCIWKK
                     NSLVLGRSPYQWQHEPCLFGWKQKGKHQWFSDRKQTTIWEYDRPKSSKDHPTMKPIQL
                     MAYPIQNSSMRGTLVFDPFLGSGSTLMAADQTGRVCYGIELDEKFVDVIVKRYMESTS
                     NRDVSVLRNGETLSYDQALEVMEEHS"
     gene            15971..17224
                     /gene="dcm"
                     /locus_tag="VBC52_000531"
     CDS             15971..17224
                     /gene="dcm"
                     /locus_tag="VBC52_000531"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEO6882136.1"
                     /translation="MTLTFLDFFAGVGGFRRGLELAGMTCLGYCEKDKFARKSYEAMY
                     DTEGEWFHDDITSIDPTRLPKADLWTAGSPCQNVSIAGKRAGLYGERSGLFFTFVDLL
                     QSQKEEDKPEWVLLENVKGLLSSGGGRDYLDYLSILDQAGYDLEWQVFNSKDYGVPQN
                     RERIYTLGHLRSRGRRQVLPLSGESGSHLKQLIGGMQSYRVYDPSGIATTLVGEGGGL
                     GAKTGLYLIDQSLTESKLTDEARCITARYTAGATKRTAMNSGVLEVQPILTPDRVIKR
                     QNGRRLKEQDEPMFTLTSQDRHGVLEGIKVRNGTKQGYQVAELGDSVDLSYPGSQTRR
                     AKVGKGIAHNLSCGGRMGAVVWNGRVVKIRRLTPRECFRLQGFSDDLFDKAQAVNSDA
                     QLYKQAGNGVTVTVVYTIGKAILSA"
     gene            17567..18016
                     /locus_tag="VBC52_000532"
     CDS             17567..18016
                     /locus_tag="VBC52_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HEO6882137.1"
                     /translation="MDDKTLERLRKTYPTGTRVRLVHMDAPYSVPIGMLGTVEDVDDI
                     GSLIVSWDNGQSLNVLHGIDRVVIVEPNRHDFIRSIYCYLRAGHNEFVFDSGDIPVQL
                     THEEVITLMTSTHPQTEIDIYIDECNHFNLKPTTVLTKEEIEKAIQE"
     gene            18068..18541
                     /locus_tag="VBC52_000533"
     CDS             18068..18541
                     /locus_tag="VBC52_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEO6882138.1"
                     /translation="MAVRGRKPKPTNLKILEGNPGKRPLPTNEVKPKQKAPRCPQWLE
                     DDAKKEWKRMGKILEQIGILTEMDMTAFAGYCQAYARWKEAEEFLSKHGSIIKTPNGY
                     LQQVPQVSISQTNLKIMLKFCEQFGLTPSARNRLATMDAEVGTGDEMEDLLGGVL"
     gene            18538..20130
                     /locus_tag="VBC52_000534"
     CDS             18538..20130
                     /locus_tag="VBC52_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006268711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEO6882139.1"
                     /translation="MTYHYEPSPFMLPTSRYDKAKADRAVTFINNLSHTKGKWAGKKF
                     DLLPWQEQIVRDLFGIVKEDGNRQFLTAYIEIPKKNGKSELAAAIALYLLYADNEASA
                     EVYGAACDRNQASIVFDVAKQMVQMSRPLEKRSKIMAATKRIVNYSNAGFYQVLSAET
                     GTKHGLNVSGLVFDEIHAQPNRHLYDVLTKGSGDAREQPLFFIITTAGTVKNSICYEL
                     HTKALDILKGRKKDTSFYPVVYGLSDEDDWNDEANWLKANPSLGHTIGIDRVREAYQQ
                     ALDNPAEENVFKQLRLNMWTSSHVAWIPEHVYAKGNAPIDYEALKGRDCYAGLDLSST
                     SDITAFVLVFPPRHSEENYIILPFFWLPEDTLELRCRRDHVLYDVWKRQGYIKTTEGN
                     VVHYGFIEAFIEQLSETYHIKEIAYDRWNATQMVQNLEGIGLTMVPFGQGYKDMSPPS
                     KELYKLMMEGKIQHGGHPVLKWMGQNVVMRQDPAGNIKPDKEKSVEKIDGIVALIMGL
                     DRCIRHQDDETSVYDERGLLSF"
     gene            20201..20458
                     /locus_tag="VBC52_000535"
     CDS             20201..20458
                     /locus_tag="VBC52_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEO6882140.1"
                     /translation="MQINIENLVSISEANQNFSKVARMVDANGTAVILKNNTPKYVLV
                     DYQSLIKEEQDTPVVVEQTTLDEVATSVLSRHLDAFKELAK"
     gene            20455..20826
                     /locus_tag="VBC52_000536"
     CDS             20455..20826
                     /locus_tag="VBC52_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001221831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEO6882141.1"
                     /translation="MRILTVEQVIELHSRLIQATGGLDGVRDAGLVESSLSSAFSSYF
                     GIEKYPSIEEKAARLCYSLVNNHAFLDGNKRIGVYVMIIFLELNGIVLNQTDEEIVKL
                     GLGVAASELDYNAILEYIRNH"
     gene            20852..21178
                     /locus_tag="VBC52_000537"
     CDS             20852..21178
                     /locus_tag="VBC52_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882142.1"
                     /translation="MTHSILSLEVENELLSSVREVCTESDLKLEELVKDYLGFLAGLN
                     ASAIEQVSALPNENEKAEWLAKYYFDARINQLQFEALKKEVLLGHDKIKESKVTRLED
                     VRHEFD"
     gene            21246..22534
                     /locus_tag="VBC52_000538"
                     /pseudo
     CDS             21246..22534
                     /locus_tag="VBC52_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769756.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            22527..23225
                     /locus_tag="VBC52_000539"
     CDS             22527..23225
                     /locus_tag="VBC52_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003084543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEO6882143.1"
                     /translation="MRKFWNFTDEGGVRTLRIEGQIADETWFGDEVTPQQFKNDLISG
                     TGDITLWINSPGGDVFAAAQIYNMLMDYQGDVHVIIDGLAASAASVIAMAGTTVSMSP
                     VAMMMIHNPWTFAQGEAKDMAKVIEMLGEIKESIINAYELRTGLSRTKISHLMDSESW
                     FNAKKAVELGFADKVLFEKEETPEQDDQNSYTFSRVTAAHDLVVKLQASLQPPKPQKS
                     IPINQLEKRLNLLK"
     gene            23239..24447
                     /locus_tag="VBC52_000540"
     CDS             23239..24447
                     /locus_tag="VBC52_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEO6882144.1"
                     /translation="MSKLLELKEKRNAAWAQAKAFLDTVRSEDGLVSDEDSKRYEEME
                     AKIELYNKEIARLERQEKIDLELAQPNSHALTTQPTVIVDNQKEDEKKGVASGIYTQT
                     FWTSIRKRHFYDVKDVLRVGEDTEGGHLVPDEYEKKLVQGLQEENFFRSLATVIKTSS
                     GERKIPVVTGHGSASWMDENGLYPETDETFGQVTLDSHKIGTAIRISEELLNDSVFDL
                     ESYMTSEFARRIGTEEEKSFLVGDGSKKPTGIFTQADVEGPTTTTKDITFDDMIELYH
                     SLPAPYRKNAVWILHDTTVKAIRKLKDNNGNYIWQPSTQAGQPDLILNRPYYTSTFAP
                     LPEAGNKAIAFGDFSYYWIADRQGRTFKRLNELYANNGQIGFLASQRVDGKLVLPEAV
                     KTLTIKGKTA"
     gene            24444..24701
                     /locus_tag="VBC52_000541"
     CDS             24444..24701
                     /locus_tag="VBC52_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEO6882145.1"
                     /translation="MMTLEEVKLYLKVENDEEDFLIQQLMATSQQLCGDILREDSTSE
                     VLKTAILYGVAYLYEHREEANHKELKETLYHLLLADRKDVF"
     gene            24701..25039
                     /locus_tag="VBC52_000542"
     CDS             24701..25039
                     /locus_tag="VBC52_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HEO6882146.1"
                     /translation="MKIAPLREQLVFQEKRLKQDDIGNESAIWDDLFMRWCSCRPLAL
                     TESDGSATKLIHNKVQFTLRYDKKVLALNSLTTRIYFRDQFYAIESIDGDTVARSLIY
                     IVATKEELYD"
     gene            25032..25400
                     /locus_tag="VBC52_000543"
     CDS             25032..25400
                     /locus_tag="VBC52_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO6882147.1"
                     /translation="MTKIGLDDLTSVIEKELTTYAKETIETMREVVEEVTDDAVDTLT
                     VTSPKRRGKYAKGWKSKATTDTNTALTKTIHNRTPGLTHLLEYGHAKQNGGRVEGRKH
                     IAPVEKKAIQSFEDKLRQKL"
     gene            25409..25735
                     /locus_tag="VBC52_000544"
     CDS             25409..25735
                     /locus_tag="VBC52_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882148.1"
                     /translation="MRFEELFPVLKKTKLPVAYHHFEEGHSPSPPFMVYLVTDSDNLG
                     ADNWAYHKAINVQIELYTTKKDLATEKTVESVLDAHRLYFDKVETYITSEKLYQTIYS
                     ITLLGG"
     gene            25738..26307
                     /locus_tag="VBC52_000545"
     CDS             25738..26307
                     /locus_tag="VBC52_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6882149.1"
                     /translation="MAEKNKVTFGLQDVHWAEVTSEGADGALTYGTVERLRGAAELTL
                     EPTGDKGSYKADNINFYTTESNDGYEGTLKVALLSQEFLTRVLGEKLDATTNTISEIA
                     SSKKKNFALMFRFEGDKKETLHVLYYCYASRPTVGSKTKSGSDINEVELTFTASPRPL
                     DKVVRRRTTEETSDEIRENWFKSVFEPAA"
     gene            26319..26738
                     /locus_tag="VBC52_000546"
     CDS             26319..26738
                     /locus_tag="VBC52_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882150.1"
                     /translation="MRQNITISGKTYPLATNAYTPIAYKEQFGKDYFQDLFNMLSAES
                     IMAQLEQLEEGEELKASQIDLSILSDFDMTFFHRLFWVFAKSANPRIKPFEDFFMSME
                     EFPLQEVGPVLMSMLNQGMTTRKKQKLPKQRARKSSR"
     gene            27033..30152
                     /locus_tag="VBC52_000547"
     CDS             27033..30152
                     /locus_tag="VBC52_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEO6882151.1"
                     /translation="MAGNIKGITIEIGGDTQPLQTALKGLNKQASEATKELRQIDKAL
                     KFDTGNVTLLTQKQEVLAKQVETTKEKLATLRQAQAQVEAQFKAGNIGADQYRAFQRE
                     VESTQTVLKGYESKLESVNRALSENGAQVETNQSKLNRLQNEQAQLVSESEKLQSSFK
                     LQESALATTASEADKLALAQQKVASHSEILEKQIHNLEQQLSLTKSEYGENSVEANKL
                     EKTLNETKTAYNNLQNEMEELASSSASSKASLEETNSLLKADLLMEFGDQLGELSQKL
                     IDFGQQSLDAFLEVDEGMDIIVTKTGATGSALEEMTDIAKTLATELPTDFNTAGSAVG
                     ELNTQFGLTGDALKSASTQLIQFSEINGSDVTSSAISAKQAIEAYGLEATDLSSVLDT
                     VTYTSQSTGVGVQELMDKAVAGAPQIKALGLSFDEGVTLMGQFEKAGIDSSAALSSLS
                     KAAVKYAGDGLTLQEGLAGTIEQIKTSTSETEALSLASEIFGSKAAPRMVDAIKRGAL
                     SFEDLAGTADKAAGIVTQTYEGTLDPIDQFTTAQNTAKLAMAEMGDAIAATLAPILEI
                     LASLLQAVATWFSGLSEPVKQFIVIVGSLVAALGLVLPIFIALQAAAMAMGTTIMGMI
                     TAAAPIVGIILGVIAVVALLVVGIQQLWQHHEGFRTAVTEIWNAIYAFLSVIIQQISS
                     FVMSIWGTLTTWWTENQQLILNAANTVWTAISTVIQTIMTILGPYLQASWENIKLIIT
                     TAWDIIKVVVETAINVVLGIIKAVMQIITGDWSGAWETIKQVVSTVWEAIKSLISIVL
                     SAIAQFISNSWNGIKGTMTNLLNSIKSVVSNVWNSIKSTISSILSSIGSTVSSVWNGM
                     KATISGVLSGISNTVSSVWNGVKSTITNAINGAKNAVSSAINAIKNLFNFKIKWPHIP
                     LPHFSVSGSANPLDWLKGGLPKISIQWYAKGGILTKPTAFGMTGNSLMVGGEAGREAV
                     LPLNNQTLGSIGRSIAATMPSKGTTITVNITDVVIREEADMKKLADYVAGRLADEMAR
                     QALLRGGTV"
     gene            30149..30874
                     /locus_tag="VBC52_000548"
     CDS             30149..30874
                     /locus_tag="VBC52_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6882152.1"
                     /translation="MIKHNELVLNGKGTSSFPFKVLVEDRPSIQVPRSKTQLLDHRGL
                     SGAIVQTNKHREVIEKSYRLYLIGASEKEVNEFSAYLMQEGFWLESERLKLTRLWCYR
                     TDSFDIKQDDHDVYVIDVTFICHPTRFFKSGDRQVLSANGVLKTQGSALAFPSITITG
                     QSVSETSFTVGDQVIRIEKFTEPLVMVNHPDRPSFKTLSGKAVKWSGDFITIDASHPT
                     QSVGVILGSGILSLTFETNWGWV"
     gene            30874..33789
                     /locus_tag="VBC52_000549"
     CDS             30874..33789
                     /locus_tag="VBC52_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6882153.1"
                     /translation="MLYLLDGQTRTAKWNGQPLFEAVSATVEEKLNGTFQLRLTYPIT
                     DSGVHETLRTDELILCPTPDLGKQLFRVKQAKIQDDTVELECSHISDDVMKRQIKPFS
                     ATNTTCQSALMRMVEVCPSDLGLFSFDSDVTERHTYVSDEDLTLYQALMDGKHSILGT
                     WEGELVRDNFQLIVKKHRGNDKGVILTSHHNLKAFEDKGDSEKVITRIYATSTFQIEG
                     SDEATVLSVVVESPLINQYPYIYEARYENNTLQTEEELRQWVMAKFTHEHIDHISRQI
                     TVEAYQLDGQEVHLGDTVTLKSQKHKVDVKKKAVGYTFDALEEVYLSVTFDDEVSFTT
                     SGSSGPHSLTSAAKTILDVHQSVTEHRASKERANFNKVFDRHFERLQTEVEDGIAKAE
                     AEGERSGKKAALDYLATDALEARVAALQKATIDELTVSSSAWMTRLVSQQILSEYVKS
                     LEVEADRVVIPGQHTPVFSLDRDGNLSIDTPLLKVRGESIVTQADLKTISLTPGPKGD
                     AGADGVGIQSREQYYLVSAQKTGLTTTSTGWSKTIPSLTSTLKYLWNYEKTTFTNGST
                     TVTTPVVIGAYGDKGVDGKAGKDGKTLYTWRMYADSDKGDGISAVSTGKRYLGLAVNR
                     ESATPSTNPSDYTWSSFFEGTELGGRNYIDDYAMKAMTFSSVTSEWKKEVIEDTSSVS
                     GVTVKMTCTKAGTGGFHRNFHDLRSRVGANMTFSIDLKASKTVSLTIGNELSGTKVVQ
                     VSSSWQRYSVSWKVTSAQNHSYVFYLKSGTWVVGDVVYLRNVQLEDGNVASAPGPSLN
                     DLIAQIDAKADNGFMKQQLDLLTEKTESLRVDLEARALAKEVADWLKSYKEFEKNNEA
                     VLAQFNQDFIDNTARIAAIEADLKANSLLLNFVNTYLRAGDNGVIIGKKDNSEVIELT
                     PQGMMIKSAGNAVMTVTAGVIKIHHGVFVETLQVGYYRLEAARHNAKHLVCRFIDAK"
     gene            33802..35658
                     /locus_tag="VBC52_000550"
     CDS             33802..35658
                     /locus_tag="VBC52_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882154.1"
                     /translation="MADYGSNNDRGYTLLLRVEETGTSTANNTSTVRVQLWLKNGYTT
                     FGMYDCRASVTINGQTLSWSGRPDMYTAHSSLHLIDKTITVPHDSNGSKTISFSATFS
                     GSGGWSPGTLNTGSQTLRLSDIPRSSSATVSGNMMGQAVTITIKRASSDFTHNMTWHF
                     GSLSGTIGTGIATSVTWMPSISQLATQIPNSTSGNGHLTLATIYGGKTIGSMTIPITL
                     NLPTSVVPTLGSISISESHATAKTILTGTSFAQLVSNPKVTFNQGAGIYGSTIPSTGF
                     RAEVFKFENNQWVQLQNVTSSNNGLLGGINWIGRAKVSAYVTDSRGRQSARKEVEITL
                     LEYFKPIFSFSAVRAGSSMNQVTVIRKLKIAPLTINSVQKNKAALTWEVVDLASGQKV
                     TNAGGAANWTSTTEHTKTDFQAILGGTYDTTKSYTIIGRLEDLFYSTTFEFTIGPEKV
                     VYGLSSSGMGIGKAWTRGVLDVDGSLPAYFDGDIYMKNKKLLDIFYPVGVIYESTSSS
                     NPSTFMGGTWERFGNGRILVGVSENESEFNSVNQSGGSKTHTLTIDEMPSHSHAQYVT
                     ANNGSGAVRRDYSSDGSSTIYPQGNNTGNTGGGKPHNNLQPYVTVYRWRRAA"
     gene            35676..36074
                     /locus_tag="VBC52_000551"
     CDS             35676..36074
                     /locus_tag="VBC52_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEO6882155.1"
                     /translation="MKELLATNKVLFSAIGGLIGSIFGEVDGFLFALMVFISIDYITG
                     LMAAAVEKKLASNIGFKGIFKKIVLLFLVAVGQIIDEHVLKQGGMVRTAIIFYYLSNE
                     GLSIIENAARIGLPVPEKLKDVLKQLKQGD"
     gene            36076..37482
                     /locus_tag="VBC52_000552"
     CDS             36076..37482
                     /locus_tag="VBC52_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEO6882156.1"
                     /translation="MTFLSKIKDGCLASWEHGILPSVSAAQAILESGWGESLLAQYPN
                     HNLFGIKASSDWKGKRVDLPTQEYIDGKFVTVEATFRKYDSWEESIKDHALFFSETAW
                     RRSHYQNVLGEEDYKKTCLALQASGYATDPNYGSKLITLIEAHHLNTWDDRILNKKGE
                     TTMSKHLVICGHGQGQTGYDPGATNPSLGITEAGKVREFANLMKKYSGNRIDYITDHN
                     VYNYRSIGSLGNGYESITELHFNAFNGQARGSEILIYSGYTADSLDQKLLAILAKRFT
                     NRGFKQVNWLYNANVSASRGYNYRLVEIAFIDNNSDVGIYEANKDSMAREFVQAITGQ
                     AQVISPSPNTPQSRVTSYHVGDPVTVQQHATHYQTGQAISSWVKGKTFKVIRVKDVNQ
                     SNSKKAYLLEGINSWVLEQDVKGTTNGHSEQTYTVQKGDTLYGIARKFKTSVSELVRL
                     NSIINPSLISVGQKLKLK"
     gene            37618..37725
                     /locus_tag="VBC52_000553"
     CDS             37618..37725
                     /locus_tag="VBC52_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019773529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEO6882157.1"
                     /translation="MTAFEVVQIIIGFGSFTVALIGLCYKIFKDDDKKK"
     gene            38033..39274
                     /locus_tag="VBC52_000554"
     CDS             38033..39274
                     /locus_tag="VBC52_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO6882158.1"
                     /translation="MAVRLIKTKSNRKKQRVCAYTRVSTTNSSQLDSLENQKAYFETF
                     YVNREDVDFLGVYYDKGISGSKEKRPSFQAMLEACRQGQIDLIHTKSISRFARNTMTV
                     LEVSRELKALGVGIYFEEQNINTLSNEGEGMLSVLASLAEEELQSMSDNQRWAFQRKF
                     QRGEMVINTKRFMGYDVDDKGELVINEAEAQIVRRIFQLYLDGMGMHRIAKLLNQENV
                     PTVTDAKWHDTTVRNILKNEKYKGTVLLQKYFHVGINWPKRLNQGQVEQYLIEDNHEP
                     IVSKEVWQAVQDKLASKTWKQGVNKHYRFTSMLKCEYCGSTLKRQVTYKKQIVWCCSK
                     YIKEGKASCRGMRVPEKAIEEWKLRTPVTVIERNEYGQKHYSYSSQESAADGHPSASN
                     QNKSGSLLSGVHRPRRTAIKL"
     gene            39219..40523
                     /locus_tag="VBC52_000555"
     CDS             39219..40523
                     /locus_tag="VBC52_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO6882159.1"
                     /translation="MAAYCRVSTDQDEQLSSYENQVSYYRDYILKHEDYELVDIYADE
                     GISATNTKKREAFNRLIQDCRAGKVDRILVKSISRFARNTLDCIKYVRELKELGIGVT
                     FEKENIDSLDSKGEVLLTILSSLAQDESRSISENATWGIRKKFERGEVRVNTTKFMGY
                     DKDENGKLVINPEQAEVVKHIYQQFLKGYSPESIAKELNTNGIKGWSGKANWYPSSIL
                     KMLQNEKYKGDALLQKTYTVDFLTKKRTENDGQVNQYYVTNNHEAIIDTEMWDTVQEE
                     LARRKDFREKHKLKTYIMQSDDNPFTTKVFCAECGSAFGRKNWTTSRGKRKVWQCNNR
                     YKVKGQIGCQNNHIDEEMLEKAFMKAVELLQEHRADVVVKWQKLEKGTNLLHKHYAKQ
                     MYQLLDLEKFDGTIMNKVLDHISISEVGQIIVIFLEGTEVEL"
     gene            40593..41366
                     /locus_tag="VBC52_000556"
     CDS             40593..41366
                     /locus_tag="VBC52_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882160.1"
                     /translation="MGENLNSNIDPRIIFKQLPPATIIKGFVAGETKCNYEIYLLELL
                     NKSVYFREKGKSKFHAPEDESHGECDAVADDYKIDFKLLSSSSRLQASSLFSPSITNL
                     GNGITAIGESRNPNGEIKATQIHAAFRFRTVSDLIRLKVKRQHVRKQCLEKDIIKVLN
                     MLEKQKNLLLFFPYIFYTEEEISTNELDDIILNAISYDFSSLFAYRHIKVQSFETYLL
                     TIVRDEFNIFKISNKRLKLIEKLSCSELPTFVKLKSYSI"
     gene            41380..>41951
                     /locus_tag="VBC52_000557"
     CDS             41380..>41951
                     /locus_tag="VBC52_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882161.1"
                     /translation="MVNPEFYEKIAKIFCGDDLELFKYKSGPDLVHFFNNNFKISDSY
                     GQGFPTRWRYVNQKLLDFSSVGKIDEYFNIILSKQYLLTERQISEIDALVHQQRILDE
                     LNKVCSVYSLKLSQKDGAFHLVEIDLDLIEIGSGGFANIYFQKSTGLVLKKLNEESAR
                     SASIRSRFKREYEITKSCSDIGSIIKVYDF"
BASE COUNT        12642 a         8123 c         9587 g        11599 t
ORIGIN      
        1 aggatttatt tttaataata aagataatac tgatttgtct acatacatgg tgccagaacc
       61 gaatggtttt attgagtttg ataattttga gttatataag ataaactatg aaattattaa
      121 taattgcgaa ttaaatcttg aaatcattgt aattgctaat gtaattgttc ggcagtatat
      181 caaaggtgag atggagattg atactaaaac taagtttgtg tctgtatatg ctgaagtaga
      241 acttgattct ggtatcaaaa actttcgaat ttacaataca gaatttaaat cagatcagta
      301 caaaaagtcc cgaaacttaa aattgagtaa agactgggtt ccatatataa ggaagaagga
      361 ctttgatgct attgctgaaa aattcttaag gaagtactat cctcaagccc tgactcaacc
      421 aacccctgtt ccagttgaga caatagtttc tgaaatggga ctgtctatcc atcaagagaa
      481 actaactata gataattcag ttttcgggaa aatggttttt aaagatactg atgttgaggt
      541 catagaggat gagcagttag tttctgagca ttttaacaaa gggagtatcc ttgtagataa
      601 ggatgttgtt ttcaaacgga atgtaggttc gtataacaat acggtcatcc atgaatgtgt
      661 tcactgggaa ctccataaag tatttcacga agtcaagatg gtacttgata aagaccactc
      721 acaagttagt agctggacag aagaaaattt agctgattct agtatgtgga cgtcgcttga
      781 ctggatggag tggcaagcta atggaattgc tccccgaatt cttatgccca aagtccaaac
      841 tagaataaag attagggagc tatttcaaac tttgacattg gttaatcctg atataagtcg
      901 ttctgaatta gtgcaagaag tggtagacaa cttagctact ttctttgagg tatctagaca
      961 ggctgctaaa attagaatga ttgatttagg atttaaggaa gcaaatggtg tatataatta
     1021 tcttgatgat agatatatgc ataactttgc ttttgaactt gaagcatttg ataaaggaag
     1081 tagttatact attacttcaa atgacttgtg ttttgagtat tgttttaacg agagttttag
     1141 acagattatt gatagaaata agtttattta cgttgataac catctttgtt taaaggataa
     1201 gaaatttatt tatatgacaa aagatgggcc aattatgact gactatgctt acgagcacat
     1261 ggatgaatgt tgtctaatct ttaaagtaaa gtctaaaaat ttcacctcga tttctaatga
     1321 gacatactat gattatgtac tgaatagagg cgtaacaaag gaaagtgaga ttaaggctga
     1381 ctttgtagat attctccaga atccaagttt gatggatcag ctccctccac tggatatgat
     1441 gaaactcggg aaaaaaatct ctgaattatt gaaagaacta ccttttgaat tttctgggac
     1501 attacgtagt catagaaagc gaaaaaattg cacacaacca tttttggcta agctagtcgg
     1561 aattacagaa cgaacgttga gagattatga aacactagaa gataatttac caaggcttga
     1621 gttaacattg tcattttgtt ttgctttaaa attaagacct gaacttagtg acgatatgat
     1681 aaagaaagca ggtcatcaac ttacaatttc tccaccacac caagtatata aaatgttgct
     1741 atcaacaagt tactataaac ccttgggtga aataaattca atattacaag ctgcagaaat
     1801 gaaaacctta taaaccatca caaatttgtg atggtttatt tttttggttt caaaaagcgg
     1861 aaagtcagct tccggttttg agattgaatt agtatgattt tactacataa aaattccaaa
     1921 aatctgttgc caagggcgtt ttaaggcgga agtttggact acctgttttt gatagaagta
     1981 aaactatata ctgagagagt aaagattaat cctacaagtt atcgtctctt taaaaatact
     2041 ttgtaatggg gaatattcct acattattgg ctttaactgt gtttttaggg aaagcagctc
     2101 aacttataaa gccagttgac catgattaag caaaggaatt gctgatgtgg tcaaatacac
     2161 aactgaatat tggtttgtca tactcttttt ctttgcttgc caaagaaacg gagtcaaaag
     2221 catgccaatc aatgtcaact ttttcaacta ctgtcaaact caatcttcat caactggtgt
     2281 ggagccatca aagggtgaag tactagcgcc aatgttcgtt caggactatt atatgtcctc
     2341 agaatattca ggactgattc gtggtacatt tcgacaatgc cgtattatga gtattcccat
     2401 tctaacagcc tttgtccctg tggcagaaga ggagtatgaa caaatggttt ggtggtataa
     2461 caactcagtc aatgattatc tcaaggattt tcgtaaacct aaaaagaata ctttagaggt
     2521 ctcctcctgg gaagtcttca ctgaaaagca ggatttagca attgctgatg aaggtattga
     2581 actatatcta gtaatggatc aactggactt cttgaaagct aaactcgcag caaccaattt
     2641 ccaagcacca gaaattttag atatgctttt tgaaggttat gagaataaag aaatcttcga
     2701 gtacctaggt gttaagaaat ctgcaggcta taagaaagtc aataatgccc agaaagaagg
     2761 gctgaaactt tacaaagaac tcaacaaata aaaccaaatc gccatctcaa ctgaggtggc
     2821 gattttttgt cagtcttttt gatagaagag acactcgtat ccatcagctc tgaggttaat
     2881 atcaggcatc cagtatggtg cttttcccat caaagttgcg atttgctcaa gattttggct
     2941 ctggtcacac tcaatgatga tttcatcatg cacatgtcct acaattctga agtctttcag
     3001 ttgtttcata gagtaagcga gtatgtctcg acttatagct tgaataatat tctcgacaaa
     3061 ttttggacca tagctttcaa gtctctccca acgtttagca gttcctgtac cttcgtaggt
     3121 gacggactct ccaccaaatt ggttttctcc cattttaggt ttgacatagg cgagtttgcg
     3181 tccagaagga agagtaataa ataacatccc ttttcttact ttgaattgaa taccatgggt
     3241 ttcagtttgc ttttggtatt ttattgcagt ctttacagct ttatcaacat cccaccaaaa
     3301 gagtacgatg tttgggttgg cttgtctcca cgagtccaca agaggctgca gctcctgttc
     3361 atcaagcccc atgtcaatag cccccatggc tttaagtgcc ccgactgctc cgccatagcc
     3421 acaggccaac tctgcaattt tccctttctg acgcaagtct gcgttacgtc catgtttctc
     3481 aacaggcact ccaaacatct ggctagctga catacagtag atgtccttgc cttgttcaaa
     3541 gaccatgctt cgccattttt cgccagctag gtgagataat actcgcgctt ctatggcaga
     3601 aaagtcacag acgataaact ttttcccatc actaggtaca aaagcagtac ggatgagttg
     3661 agatagggta tcttgcgtac catagaggag atcagttgcc tctaagtcac cagttttgaa
     3721 gaggtctcta gcctctttaa ggtcaggaag atggttctgt gggaggttct gaagttgtac
     3781 caatcgccca gcccaacgac ccgttcgatt tgctccgtag aattggaaca tccctctagc
     3841 tcgaccatcc aaacaaacac agttcaccat agcttggtat ttagaaacgc tagatttagc
     3901 cgcctgttgt cttaacttta ggactttagt tgtctttgca tcgactacct ttaatagctc
     3961 tttgacagct ttcttatcaa gagaattagt cgttactccg tgttctcgta gccagccaat
     4021 catttgaaga acagagttgg gattttcaag cccagtaagt tctttaagtt cctcttgaat
     4081 tttagatttg ctttcctcgt caatggtaat agctgctttg acaaagtcta catcaatacc
     4141 aatcccacta tcattgatat tttggtcttg atgatactct tcccaaagaa agtcaggaac
     4201 agggtggtta cggagtttat ttttgatggc taactcaacc tcaacgtcac gcttgttgta
     4261 gttgataaag gctgcccact tatcaggtgc gtgatgaggg aagtttcgtt ttcgtccacc
     4321 attaattttg gtaggtttgt agggcaggca gaagtaacga atcacatccc cgccttcttt
     4381 taacttctgt tctttgagtt ttagaactgt tccgactcct tcaagggaga gtggaagtcc
     4441 aagatatgct gaccaaacta tgctacaacg ccatgagttt ggagacagaa agccctcgga
     4501 caataatttt ggatgatgtt tctttagcca gttagacaga cagatgcgtt caaatgaggc
     4561 attaaaagcc cacttaataa tggagtcatt caccaatgct tgtaggattt gatcaggaag
     4621 attttcttta gttaggtcat aaactgttac tggaccattg tcaacagaga ctgctaagag
     4681 aaggatttca aaagaatcat cttctgcgta gctataaaca ccagactttc tcaaatctac
     4741 ctcacagtag gtctctaagt cgatggatag ttcttttatt agcatagctt gtcctttcat
     4801 aaaaaggtgg caggagtggc tgccaccgga ggtgttatcg gctacgatgt agcggtgttg
     4861 tgttgttgct atgatgtttc ttttcagcct tacgttcttt ttcacgacga atatcatcac
     4921 ggatggtcat gtagttgagg tacagaccaa agaataggta aatgccaata agtgagtaaa
     4981 agaaaattgt ttcaaacata tttagtcctc cgttcttagt tcaagaaatc atcatcgtca
     5041 tcagtcgcaa agtcgtcttc agcacgagta cgtccaccaa gtggctctcc atcacggagt
     5101 ttttgcaagt tattcaaacc acaggcgata cccttgttac cgttagagtt gaaggcatag
     5161 aaggaaatag acgcacggcc atagatacct gagtagagtt cagaagtatc tataatctct
     5221 tgtcgattag cgtccacaac cccaggctta tgtggtgagt tggcattgac gaagtaggca
     5281 ttgcgataag cttcatcatc aggacgctct aagtctccat cacgaagtgg agttttaagg
     5341 atagaaagtg ctgggacaga ttttccatta cctttgagtt tggactcgcc ttccttgtag
     5401 gcaagctcaa tggctgcttt gattttatcg acagtttcaa tatcatcctt tggaatgata
     5461 agagagacgc tgtactttgg cgtgcttccg ttgatggact taggttcatt ggcattcaag
     5521 tagctgaagc gtgtgttttt accagtgatt acttttgtag tttgtacttt agttgtcata
     5581 cgtttaatcc tctttaaatt cattagtagc taggttcaat tcttgacgac tgtcgtcaag
     5641 aggaacgagt gttggtttac cgctttgttt tacaatcaga ccaccaagca ggtcgttaaa
     5701 ggttttcttg ccaaggagtt tagtcatggc agtgatagtt aggagtttct tttcgtaagg
     5761 atcaaagcca gcatccatca cagcttgact gacggcagct tcgtctgaaa acttacgaac
     5821 tgagcgtccc tctactaatt tgtagcctgg aatggagtgt ccctcagtag cttctttcaa
     5881 agcataggct ttgacatcgt ttgcccatga aaccagtaag tccagtttag gcaaaatctc
     5941 tgcaatatcc tcatagtcta gagtagcagg atccgcaaac tccatcttgg caagtgctaa
     6001 attatcctcc gcacgtttgc gacagacatt cttaatcttg cagaactggc agtgtttacc
     6061 agatttcagt tctccctcgc ctttaaaggc aagttcagct ttaggtgaga gctggtcttc
     6121 cgcccattca agcagttcag ccttatccat ttcaaaggta gagatattgt tcttccgtgg
     6181 ttgaaagata gtcatggtga cattatcaaa atcatagatt ccatcgaaca tctcaagagc
     6241 acctagggca tagcacatca tttgtgggtt gtggtcggca tcgaccagaa tgcccaaacc
     6301 gtgcttgtag tcgatcactt gaagaagtcc atctgccaca atcagacagt cgccagtacc
     6361 gaacccttca ggtacccatt tggagaagtc caaacgttgc tcgataagta ctgttgggtc
     6421 atgagagtag tctttagctt tctcgacctg ttccatgacg tagttgcgat attcctcggc
     6481 acaattttgc atctcttcat tgtagaagga cagatcctca gttggatcac gcgcctttct
     6541 gcctagtgcc ttctcaacaa ggtaagcaca gagctcgtga gcgtcagttc cctcaagggc
     6601 aaattcagaa gtaacatctg gcatgtcctc ggttaagcga acagaaggcg gacagttgag
     6661 ccagcgatgg gatgcggaag cagataggac agcatggtta gtcatggcca attcctccag
     6721 cttcttcaag aaccgctgcg tagtgctcag ttgctaaggc tgatagggat tctgcaccat
     6781 atttattgag aagagcacga acttcgttct tgtagccatc tttggctttt gtcgcaagga
     6841 cggaacgaac gtcttcgagt ttgagttcct tttgtggttt atgttttggt tcttttgaag
     6901 ttgttgtagt ttcttcctca gtagagagga tcttcttgaa ctcatctact aagcgaagat
     6961 agtatttggc tgtgccttcc atttcattaa tcagttcatt cagttgtttc attttgctca
     7021 tgttgtcttt cctccgtaat tttccgagcg agtagttttg agataacgct gatggctatg
     7081 agggtatcag ccacatcatc atcaggttcg atgtatggtt cgtttgccat attgggtcct
     7141 cctatcttac taagtaaggt tttgagtgga ttttccactt tggaagagat ttttttattt
     7201 tttatctctt acaccatact aagtagggtt gagataaatt tttccgcctg ataagaaaaa
     7261 atttgctcat caaaaaaagt ttcctgtgca tttaatagga aacttttatt tttttagaaa
     7321 aaattttctg ttagtgcgga aaatcctgtc ggattcttac ttagtaagtt aggaagagta
     7381 attcctaatt caacaacgaa agatggaggc atgatgcatg caatttacct tatctcattc
     7441 agggcaatct ggtgttcaaa caacaacggt ctacccgcac caagtgacaa tcacggatga
     7501 aactagtcta aaacgtattg ctcagtacga ccatgtggca ggattgtttt ccaataacac
     7561 tcgatcgaac gctaatttcc ttaagtcaga tgtattagtc atggatattg ataatgacca
     7621 tacagaaaat catgatgatt ggattacaga ggaataccta aaaatactgt tttcggatta
     7681 ccattttgct ttggttacaa gtcgaaacca catggttcaa aaaggcagta aggtagcaag
     7741 acctaaattc catatttatt tccagatcaa tgaagtcagt gataaagatg cctatgcctt
     7801 tctaaaagaa gagatgacga atcggtataa cttctttgat acgaacgcca aagatgcagc
     7861 acgtttcttt tttggtaatc ccaatgcaca agttttatgg aatgactcat ggctaactat
     7921 agatgctgat ttattggatt catcatttga tgatgaagaa gatttcgatg cagatttcta
     7981 tcaaccacca acaggaccaa tcacagaagg tagtcggaac tcaaccatgt cggtttttgc
     8041 ggcgaaaatc cttaagcgat taggtgtaac aaaagaagca cgagacggct ttgatgaaca
     8101 agctctcaaa tgtgttcccc ctcttgagaa agcagagctt gataccattt ggggcagtgc
     8161 tgttcgcttc tataacaaaa ccatcaaaaa ctcaaaagat tataaatctc cagaagagtt
     8221 tcaacgtggg gagatgcaac cagatgatta ttcagatatc ggagaagcag gtcttctcgc
     8281 tagagagttt ggtgacaaga ttgcctttac tagggaaaca gactacctcg cttacgatgg
     8341 taagcactgg gtggaagatg agcagttagc catgcgtcaa attcatcagt tccttgatat
     8401 gcagctagaa gttgcagtaa ccaagcttaa taatgccact caaaaactga agcaatctgg
     8461 tattcctgaa atgctaatcc aacaaggtgg taaaaagcta gaaaatgcga ttgagacacc
     8521 aattcaactg gttgcttatc aggactataa gaaagcattc gagttttata agtttgttat
     8581 gaaataccgt gactatcgaa atttatcagc aatcgccaaa atggccaaac atatggttcg
     8641 catgagtgtg tctgatttag ataagaatga actcttactg aatactccag aggcgactat
     8701 tgatttatca caaggcttgt ctggtattag ggaacatgat tctgctgatt acttaaccaa
     8761 aatgaccaat gcctctccga gtgataaggg agatggtctg tggcaggaga cccttgcgac
     8821 cttcttttgt gatgatacag atcttatcaa ttatgttcaa gagattgtag gcatggctgc
     8881 tattggtaaa gtttatcaag agcatatgat tattgcctac ggtagcggtg caaacggcaa
     8941 gtctaccttt tggaacacca ttgcaagagt tcttggtaac tattcaggaa aactctcggc
     9001 tgaagctctg accatgagtg tgagacggaa tgtcagtcca gaaatggctg aacttaaagg
     9061 gaaacgtctt atcattgctt cagagatgag tgagggaatg cgattgaata cagccatggt
     9121 taaacagctc tgttcgacag atgaaatttt agctgagaaa aagtacaagg cacctttcca
     9181 ttttgtccca tcacatacgc tggttcttta cactaaccac ctgcctaaag taggtgcgaa
     9241 cgatgatggg atttggagac gactgattgt cattcctttt aatgccaaaa tcactggtcg
     9301 gtcagacatc aaaaactttg cggactacct ttacaacgaa gcagcaccag ctatcatgtc
     9361 ttggattatt gagggagcag aaaaagccat caaagcaaac ttcaaattga tcttgccaca
     9421 agtagttgcg gactctgtct cagcttatcg agaagccaat gactggatgg gtcaattcct
     9481 tggagattgt tgtgaaatcg gtgaccactt gactgagaaa tcaggagaac tttactctgc
     9541 ttatcgagct cactgtgctc gtatcaatga gtacacgcgc agcacgacgg acttttatac
     9601 agctttggca aacgctggat tcaccaagaa aagaactaat aagggtgtca tggtaaaagg
     9661 tctacagttg aaattagaag atgattttct tgattaaccg tgaaaatcag tgaaagttgc
     9721 ttttatcatc agagggtgtt ggtgtaagcc gtgatagtca agtcacaact taaatctttg
     9781 aaacacggag agttattatt cgttggtagt gagaagagtt tagacaaaac tatcacgact
     9841 ttcacgggtc ttaaaaagtg caagtcgaga aggtcatata caaaagttgt cataggggat
     9901 ttcaaagtgc aggtttagta ggtcttttct ataacttaga tataaagaaa aataaaaaaa
     9961 gataaaaaga ggtataggaa agtaatgtaa ctgactagcg tgaactgcac ttttcctttg
    10021 gcgatgtttc tgacggaagg attgggtatg cgtgaaaaaa ttgttgaaca gaagttagtg
    10081 agtgaagtga aaaagcgtgg tggggtttgt cccaaatggg tgtcgccttc gtttggcggt
    10141 gtgccagaca ggttggtgtt tttacccaag ggtaagtttg gcatggtgga agtaaaagcc
    10201 cctggtggaa agccacgttt actacaagtg acaaggcaca agatgtttga cggattgggt
    10261 ttcaaggtac atgtgctaga tagtgttgag aagattggag aagtgctaga tgaaattgag
    10321 tttacatgat tatcaagaag tcaccaagga ctttatcata aggacacctt atgcggcagt
    10381 catcctagac atggggatgg gaaagactgc cacaacctta tcagccatca acgagctgat
    10441 gtttgaccgt tacgaggtct caaaggtctt ggtgattgcg ccccttcgtg tcgcaaacac
    10501 ggtgtggagt gatgagattg agcagtggga ggaactaaga cacctccgtt atgccaagat
    10561 tgtgggaaca ccaaaacaaa ggcgagctgc ccttgagcaa gatgcggata tctacatcgt
    10621 caaccgtgaa aacctcccct ggctggtgga acaatgtagt ccctatttca agtgggatat
    10681 ggtagttatt gatgagctat cgtccttcaa gtcttggcag tccaaacgtt tcaaggcatt
    10741 catggtcatg cgtccctaca tgaagcgtgt cgtgggattg actggcacac cgagttcaaa
    10801 tgggcttatg gacttgttcg cagagttcaa ggtcattgat ggtggcgagc gtctggggcg
    10861 attcatcgga gagtatcgta gtcgctactt ccgtgaggga gctcgcaatg gtcaggtggt
    10921 ttataactac ctccctatgg attatgcgga gtgccaaatc tatgacaaga ttgacgacat
    10981 caccatttcc atgaaagcta tggattatct tgagatgcca gaactgattt cgaccaagaa
    11041 ggtggttcat ctcactgaca aagaaaaatc tgattataag cagttcaaga aagattacgt
    11101 acttgcagac cttgagaatg gcgaggtgac tgctgccaat gcggcaagcc tttcaaacaa
    11161 gttgctacaa atggcaaatg gtgcggttta ctcaaacgac catcaagtgg tgagcctaca
    11221 tgaccaaaaa ctggatgctc tggaagatat catcgaagct gccaatggtg agccagttct
    11281 tgtggcctat tggttcaaac atgatgttca gcgtattgag gaacgtttgg taaagctcaa
    11341 ggttaagggc acaatcctta aaactgagga ggatattcgt gagtggaata aagggaaaat
    11401 ttctgtcggc ttattacatc cagcaagtgc tggacatggt ttgaacttgc aaaagggtgg
    11461 tcatcacttg gtatggtttg gtttgatttg gtccttggag ttgtaccaac aaaccaacgc
    11521 tcgtctgtgg cgacaagggc aacaagcaga tactgtggtc atccagcaca ttgtaacgca
    11581 aggaactatt gacgaggaaa tcctcaaagc actggaaagc aaagacgccc agcagtcacg
    11641 gctgattgaa gccgtcaaag cacaagtagg aggaatgaat ggatagaatt gagtatttga
    11701 tgaaaaacta tagcgatgtg aagttgaagc ttgcattggt ggaaaatcaa cttctaaact
    11761 ttcgtcccat tagcgaggag agtgtgattc agtcattggt ttatgaaaaa ccagatatgg
    11821 agagggtaaa gacttctcag atcaattcac gttcagagac tattgctctc agtttcagag
    11881 aaaaattgga aaaagaaaac aaggaatatt gggatagttt gatggagtgc tatcattttc
    11941 taaagactga actcgagttc tttgaaagta tggtcaacct tataccagat gacttgaagc
    12001 agttttcgaa ggacttgatt ttcaatgaga tgagctggga cgatatatct agccactatg
    12061 agattagtcg ttccacaatt tcttaccgta aacgtaaggt gcatcagcaa ctaaagaaat
    12121 gttatggttg gatgagtagg agtattgatc ttgatgaatc agcgtttcaa attcctcttt
    12181 cgaactaatt tcgaactgtt ttcgaaccaa atttgtacca aattcgaact gtttttattt
    12241 tgagactgtg ctatacttaa gatgtcaaaa aagataaaaa ttttcccagt aatgactgga
    12301 taaccttccg cttgtacggt aatatacagc tacaaaaaca agaggagaac ctatcatgaa
    12361 aacgaaattg acaaaacgcc aacaagaaaa actcaacgct cttttaacag cgattgctca
    12421 agaagaactt ttggttgaaa ccttggaaac acgaaaaagt gataacctag atttctacga
    12481 tgtttcggtt tggggagtta aaagagcact cgagagagcc tacgaagcag gccaacaatc
    12541 agtaaaataa atcaaagcct agccctcaag ggttgggctt tttgcgtgga ggaaaacatg
    12601 atttttacca gtgaacaagt ttcaagtgga caccccgaca agctctgtga ccaaatctca
    12661 gatgctattg tgacgaaatg tctcaagcat gataagaaca gccgtgtggc ggttgaaacc
    12721 ttaatcaaag ataaccaagt ggttgtggca ggtgaagtct caaccaagca cttatttaac
    12781 cttgaaggta ttgtcaaaaa ggttcttgaa ccttacggta tgacagatgt catggtgacg
    12841 aaccttcttg gagttcaaag cccagacatc gctcaaggtg tggataaggg tggtgcaggt
    12901 gaccaaggga ttatgtttgg ttatgcgacc gatgaaacac ctgagttctt accattacct
    12961 tatgtacttg cgactcgagt gcttgagaaa ctgatgaacc ttggtcatcc tgccttggtc
    13021 aaagatgcca aagctcaagt cacatacgac tatgaaaaca agtgtatcga gactttccta
    13081 gtgtctatcc aacacgacga ggaggttgac cttgcctcag tcaaacgaat tgtcacccaa
    13141 gctatgatgt cggttgctca acgctaccgt cagaaccttg acttcagggt tttggttaac
    13201 ccaactggtc gctttgttct tggtggctct tacgctgatg ctggtgtgac tggtcgtaag
    13261 attgtagcag atacctacgg tggctttgcc tgtcacggtg gtggagcttt ctctgggaaa
    13321 gatccaagca aggtcgaccg ttcagccgcc tacatggcac ggaagattgc aaaggacttt
    13381 gttcgtgaag ggtatgccaa acgctgtgaa gtccagttag cttatgccat tggggttgcg
    13441 gagcctgtag gtgtttatgt gaacatcttt gggacaagtg attatcctct tgaacaacta
    13501 gtaggagtgg ttcgtgaacg ctatgatctt accccacaag gaattatcaa ggagctgaat
    13561 ctcttggatg tggactacac caagaccact tgccttgggc atttcaccaa gccttatctt
    13621 ccttgggagc agtaagatgc cacgtcgacc aagcacccct tgcaagcagc atggttgtcc
    13681 taaccttgtg acctatggaa acaaatactg tgacgaccac aaagctaact acagccttga
    13741 tgccaagtca actaaagcca aaggttacga tactcgatgg aacaaggcac ggcttcgtta
    13801 cctcaagttc catcctctct gtgtttactg ccaaaggaaa ggacgactga ccaaggcaac
    13861 ggtggttgac cacatcactc cccaccgagg tgaccaagac ctcttctgga atccatccaa
    13921 ctggcaagcc ctctgtaagt cttgtcatga ccgaaagacc aagacgactg accgatatgt
    13981 ggagtatact tatcggctct aaatttagag tttcgttaca aaagtatcta atttttaacc
    14041 ctatggggga ggggggatga aatctctaaa accttgggag actaagaccg acgccccctc
    14101 aaaagtgcat tttcgcaaaa ttcgttaggg gcatacccaa aacagttaaa aagtgcattg
    14161 aaccattatg gtgtcaatct ttctagcagg gtaagtatcg tttgccagtg tttgttttgg
    14221 cctgtttttg aagggataaa tacatgaaaa atagagaaat taaccatttt tagttcttgg
    14281 aggaaaacaa atgaacgata atcagcggcg aggcatttgg aaacttaggc gagatggatt
    14341 tggctatggt gccattgctc aaatgttaaa catgtctctt ggttcggtga agcaatattg
    14401 ccgtagacac ccaaagttaa aaggaatggg tcagtttgtc aagtatcagc ttgatgaagg
    14461 agaacgttca tattgtaaga actgcatgaa aaagttacac catgctgtcc aaggcagacc
    14521 taaaaagttc tgctcaaata ggtgtcgagc tatctggtgg cgaaaccatc aaagccaaca
    14581 tgataaaacc aaaactgcat acgatgaatt gacttgccaa aactgtggca ggtctttttt
    14641 atcctatgcc aatccaacaa ggaagttctg cggacaccct tgttacgtag aacatcgatt
    14701 tagaaaagga gtagtgcatg acaactcaac ccaatatgga aattaaggaa ctccccctaa
    14761 gtgacttaaa gccggcctct tataaccctc gaaagaaatt gaagaagggc gataaggagt
    14821 acgaaaagat taaacagagt ctactcaagt ttggctacgt tgaccccatc attgtcaatg
    14881 acgatttgac ggtcatcggt ggtcaccaac gcctaacggt tcttaaagac ctcaagtatg
    14941 aaaccgcaaa atgcgtcatt gtctctcttt ccaaggaaga tgaaaaggca ctgaatattg
    15001 cccttaataa aatcactggt caatgggatg accagctcct cgctgacttg cttttggact
    15061 tgcaagagtc tgatttcaac cttgatttga ctgggtttga gccaccagag attgatgaca
    15121 ttctgtccaa catccatgac aaggacttgt ctgaagacga tttcgatgtg gatgaggagt
    15181 tgaagaaacc aacggtcgca agacgtggtg acatctggca actcggaaaa caccgagtga
    15241 tttgtggtga ctccaccaaa tcagaaaact acgagcaatt gctgggggat aagaaagcca
    15301 atcttattgt gacggatccg ccttataacg tggacgttga agagaccgct ggaaaaatcc
    15361 tcaatgacaa tatgtcggac agggacttct accagttcct ttttgatatg ttcactcaag
    15421 tggaaagtca tatggaagcc gatgcgtcta tctatgtttt ccatgcaaac acggaaggtc
    15481 tgaatttccg gaaggctttt aaggacgctg gtttctattt aagtgggagt tgcatttgga
    15541 agaagaactc actggttctc ggacgtagtc cttaccaatg gcaacacgaa ccctgtctct
    15601 ttggttggaa acaaaaggga aaacaccagt ggttcagcga ccgtaagcag acaactatct
    15661 gggaatacga ccgtcctaag tctagtaaag accacccaac catgaagcct attcagctta
    15721 tggcttatcc tattcaaaac tcatccatgc gaggaacgct tgtttttgat ccatttcttg
    15781 gttcagggtc aaccctcatg gcagcagacc agactggtcg agtttgttat gggattgagc
    15841 ttgatgaaaa gtttgtggat gtcattgtca aacgctacat ggagtcaact agtaatagag
    15901 atgtgtcggt actccgaaac ggtgagactt tgagctatga ccaagcctta gaagtaatgg
    15961 aggaacactc atgaccctaa cctttcttga tttctttgca ggagtgggtg gctttcgtcg
    16021 tgggttggag ttggcaggga tgacctgtct tggctactgt gaaaaggaca agtttgcacg
    16081 aaagtcctac gaagcaatgt atgatacaga aggagaatgg tttcatgacg acatcacaag
    16141 cattgacccc acacgacttc caaaagcaga tttatggact gcgggaagcc cttgtcaaaa
    16201 tgtgtctatc gcaggaaagc gagcaggctt atacggtgag cgaagtggac tcttttttac
    16261 atttgttgac ctcctccaga gccaaaaaga agaagataaa cccgagtggg tactccttga
    16321 aaatgttaag ggacttctat caagtggcgg aggacgagat tatctcgact atctctctat
    16381 cctggaccaa gcagggtatg acctcgaatg gcaagtgttc aactcaaaag attacggagt
    16441 tccccaaaac cgagaacgca tctatactct cggacatctt agaagccggg gtcgacgaca
    16501 agtattacct ctcagcggag aaagcggtag ccatcttaag caacttatag gtggtatgca
    16561 gagctaccga gtctacgacc ctagtggcat tgccacaacc cttgttggtg agggtggagg
    16621 actgggagct aagacaggtc tttatctgat tgaccagtct ttgactgaat caaagctaac
    16681 tgatgaagca cggtgtatca ccgctcgcta cacggcaggt gctacaaaga gaactgccat
    16741 gaactctggt gtccttgaag tccaacccat tctaacacca gacagggtaa taaaacggca
    16801 aaatggcaga cggcttaagg aacaggatga gccgatgttt actcttacct ctcaagaccg
    16861 acatggtgtt cttgagggta tcaaggtcag aaatggcaca aagcagggct accaagtggc
    16921 agaacttgga gattcagttg atctttccta tccaggctca caaacgagac gagctaaggt
    16981 tgggaaaggc atcgcccaca atctatcttg tggtggacgg atgggagctg tggtttggaa
    17041 tggtcgagtc gtaaaaatca gacgcttgac tccacgagag tgcttcaggc tccaaggctt
    17101 ttcagatgac ttatttgaca aagctcaggc tgtcaactct gatgcccagc tctacaaaca
    17161 agctggtaat ggcgtgacgg taacggtggt ttatactatt gggaaggcca ttttatctgc
    17221 ttagactagt agctaatcac tggatataag gtgaacctta cggtaatatg ctgttaccaa
    17281 aagacaaagg aagtcaaaac gatgacaaaa aatgagttca acgaaattat tgatagctgc
    17341 tttattcacc taactgtaat gaaacaacat tacactaagc cgagaaatta ttcactagat
    17401 gtgattgaat aaggaaatct tgaccaaatt aatgatttat tgaatgatat tacaaatggt
    17461 atcgaacttg gtgggtttaa cgaactggaa gcccgctata tttacgaaga caccgaagtg
    17521 ttatgggccg aggtatctca aacctttgtg agataaggag aagcaaatgg atgataaaac
    17581 ccttgaacgg ttaagaaaaa catacccaac tggtactcga gttcgtttag tgcatatgga
    17641 cgccccttac agtgttccaa taggtatgct aggaacagta gaagatgtgg atgatattgg
    17701 atcgctaatc gtttcttggg acaatgggca gagcttaaac gtgttgcatg gtattgatag
    17761 ggtggttatt gtagaaccaa ataggcatga ttttataaga agtatttatt gctatttaag
    17821 agctgggcac aatgaatttg tttttgatag tggtgatatt cctgtacagt taacccacga
    17881 agaagtcatc actcttatga ctagtacgca tccgcaaact gaaattgata tctacattga
    17941 tgaatgtaat cattttaatc ttaagccgac aactgtcttg acgaaagaag aaatagaaaa
    18001 ggcaatacaa gaatagacaa ggtttcgcta agaagccttt ttcttgtgcc aaaaaggggg
    18061 tgagaccgtg gcagttagag ggcgaaaacc aaagcctaca aatttgaaaa tacttgaagg
    18121 aaaccctggg aaacgacctc tacctactaa cgaggttaaa cccaaacaga aagccccacg
    18181 ttgcccacag tggctcgaag acgatgccaa gaaggaatgg aagcggatgg gcaaaatcct
    18241 cgaacagata gggatattaa cggagatgga tatgacagcc ttcgcaggct attgccaagc
    18301 ctatgcccgt tggaaggaag ccgaagagtt tctctctaaa catgggtcta tcatcaagac
    18361 tccaaatggt tatctccaac aagtgccaca ggtatctatc agccagacca atctgaaaat
    18421 catgcttaag ttctgtgagc agtttggtct gacaccatca gctcgtaatc gcttggcgac
    18481 catggacgca gaggttggca ctggtgatga gatggaggat ttattaggag gtgttttatg
    18541 acctatcact acgaaccgag tccctttatg cttccgactt ctcgttacga caaggcaaag
    18601 gcagatagag cagtgacctt tattaataac ctatctcata ccaaggggaa gtgggctggg
    18661 aagaaatttg atttgttgcc gtggcaggaa cagatagttc gtgacctctt tgggattgtt
    18721 aaggaagatg gcaatcgtca atttctgaca gcctatatcg agattccaaa gaaaaacgga
    18781 aagtctgaac tggccgctgc tattgctctt taccttcttt atgcagataa tgaagccagt
    18841 gcggaagttt atggtgcagc atgcgaccgt aatcaagcct cgattgtttt tgatgtcgca
    18901 aaacagatgg ttcagatgag tcgtccgcta gagaaacgtt ccaagattat ggcagcaacc
    18961 aagcggatag tgaattattc caatgctgga ttttaccaag tcctctcagc tgagacagga
    19021 accaaacacg gactcaacgt gtcaggtctt gtctttgatg aaatccatgc ccaacccaat
    19081 cgccatctct atgatgtttt aaccaagggt tctggtgatg ccagggaaca acccctcttt
    19141 ttcatcatca caacagctgg aacggttaaa aactccatct gttatgaact ccacaccaag
    19201 gcacttgaca ttcttaaagg tcgaaagaag gacacgtcct tttatccagt ggtttatgga
    19261 ttgtctgatg aagatgattg gaatgacgaa gccaactggc taaaagccaa tccctcactt
    19321 ggtcatacca ttgggattga ccgtgttcgt gaagcctacc aacaggctct tgataaccca
    19381 gctgaagaga atgtctttaa gcaactccgt ctcaacatgt ggacgagttc ccatgtggct
    19441 tggattcctg aacatgtcta tgcgaaaggc aatgccccca tcgactatga agctctaaaa
    19501 ggtcgtgact gttacgcagg gcttgactta tcgagcacct cagatatcac cgcctttgtc
    19561 ttagtctttc cgccacgaca tagcgaggag aactacatca tcctgccttt cttttggtta
    19621 ccagaagata ccttggaatt gaggtgtcgt cgtgaccacg tcctttatga tgtttggaaa
    19681 aggcagggtt acatcaagac aacggaaggg aatgttgttc actatggatt cattgaagcc
    19741 tttatcgaac aactctctga aacctaccac atcaaagaga ttgcttatga ccgttggaat
    19801 gcgacgcaga tggttcagaa ccttgaaggc ataggcttaa ccatggtgcc ctttggacaa
    19861 ggctataagg atatgagtcc accgtccaag gaactctaca agctcatgat ggaagggaaa
    19921 atccaacacg gtggtcatcc tgttctaaaa tggatgggac aaaacgtggt catgcgacaa
    19981 gatcctgctg gcaacatcaa gccagacaaa gaaaaatccg ttgagaagat tgacggtatt
    20041 gtggctctta tcatgggact tgaccgttgc attcgccacc aggatgatga aacgagtgtc
    20101 tatgatgagc gaggactttt gagtttttag gataaaaaca acctaaatag gttgaatgta
    20161 tcctaaaaag tgataaaata gagtaaagga ggaaatagct atgcaaatta atattgaaaa
    20221 cttagtctct atttctgaag cgaatcaaaa cttctctaaa gtagcccgca tggtcgatgc
    20281 taacggtact gcagtcattt tgaaaaataa cacaccaaag tacgttttag tagattacca
    20341 aagtctgatt aaagaggagc aagatacacc agtagttgtt gaacaaacaa cattggatga
    20401 ggttgcgact tcagtattat cacgtcatct tgatgcattt aaggaattgg ccaaatgaga
    20461 atactaactg ttgagcaagt tattgaactg cacagtaggt taattcaagc tactggcggt
    20521 ttagatggcg tcagagatgc tggtctagta gaatcatctt tatcttcagc ttttagttct
    20581 tattttggta ttgaaaaata tccaagtata gaagaaaaag ctgctagact ttgttattca
    20641 ttggttaata atcatgcctt ccttgatggt aacaagcgta ttggagttta tgtcatgatt
    20701 attttcctcg aattaaatgg tatcgtttta aatcagactg atgaagaaat tgttaagcta
    20761 ggtcttgggg ttgctgcgtc agaattagat tataacgcaa tcttggagta tatcaggaac
    20821 cattaattaa tagttaagcg aggtgaatag aatgactcac agtattttgt ctctagaggt
    20881 tgaaaatgag ttactatcaa gcgttcgtga agtttgtact gaatctgatt tgaaactaga
    20941 ggaacttgtt aaagactatt taggattttt ggcaggttta aatgcttcag caattgagca
    21001 ggttagtgca ttacctaatg aaaatgaaaa agccgaatgg cttgccaaat actatttcga
    21061 tgctcgaatt aatcaattac aatttgaagc actgaaaaag gaagtattac ttggacatga
    21121 caaaatcaaa gaaagtaaag taacacgtct tgaagatgtc agacatgaat ttgattgatg
    21181 tataattaga ctgaaagcac ttcaaaatga tgtgcttttt tcgtacccaa aaataaggag
    21241 gtcaaatggg tattttagat tggattggat cgaagcgctc aagagataag ccacataata
    21301 gttatgaggg acaggatttt tcctatctct ttggacggac gaccagtggt gaaaccgtag
    21361 atgagtttaa ggctatgcag acgacggccg tttatgcttg tgtgcgtatc cttgctgaag
    21421 cagtagcgtc acttcctatt cacgtctatg aacggacaga aactgggaag gagaaaaagc
    21481 tggaccaccc actgtacttt cttcttcatg atgagccaaa tccagaaatg tcatccttta
    21541 tttttcgaga aaccatgatg agtcatttgt taatatgggg aaatgcatat gtgcagatta
    21601 tcagagataa gggtggacga gtgattagtc tctatccact cttgcctgat aagatgtctg
    21661 tccatcgtga tgatagtggg agactttact acaaatacca gcgccagaca gaagaaaacc
    21721 ctaatttcaa tgataaggga actgtcttat tgaagcagga agatattctt catgtgcctg
    21781 gtcttgggtt tgatggcttg attggttact caccaattgc gatggctaaa aatgcgattg
    21841 ggatgaccct agctaccgaa aactatgggg catctttctt taaaaatggc gctaacccag
    21901 gtggtgtttt ggagcaccca ggtatcctta aagaccctaa acgagtgagg gattcttgga
    21961 atgccgttta caatggggtc acaaatgccc ataaagtggc tgtactcgaa gaaggcatga
    22021 agtatactca agtgggaatc ccccctgaag aagcccaatt tttacaaaca cggaagtttc
    22081 agattaacga aattgcacga ctttaccgta tcccaccaca catggttggt gatttggaga
    22141 agtcgtcttt ttcaaatatc gaacaacagt cacttgaatt tgtcaaatat accttagacc
    22201 cttgggtagt tcgtttagaa caagctttca agaggtctct ttttttaccc gaagaaaaaa
    22261 acaatacttt gttaaattca atgtggatgg tctacttcgt ggggattacc aaagtcgaat
    22321 gaatggctat gccatcgcaa gacaaaacgg ttggctgtca actaatgaca tccgtgaatt
    22381 ggaagatttg aacttgttgt cagatgaaga aggtggaaac ctctacttga ttaacggcaa
    22441 tatgaccaaa ctagaagatg ctggtggctt tatgaaacaa cctacggaaa ctgagccaac
    22501 tgaaaacgca acagaggagg aagtagatgc gtaaattttg gaattttaca gacgaaggtg
    22561 gcgtccgtac ccttaggatt gaaggacaga ttgcggacga gacttggttt ggggatgaag
    22621 tcaccccaca gcaatttaag aacgacttga tttctggaac aggcgatatc accctatgga
    22681 tcaatagtcc agggggtgat gtttttgcgg ccgctcaaat ctataacatg cttatggatt
    22741 atcagggaga tgtccatgtc atcattgatg gtctagctgc gagtgctgcc agtgtcattg
    22801 ccatggcagg gacaacagtt tccatgagtc cagttgccat gatgatgatt cataatcctt
    22861 ggacgttcgc gcaaggtgaa gcgaaagata tggccaaggt cattgagatg cttggcgaaa
    22921 ttaaggagtc cattatcaat gcctatgagc ttagaactgg actttccaga accaagattt
    22981 ctcatcttat ggattcggaa tcttggttca atgccaagaa agctgtggag cttggttttg
    23041 cggataaggt gctctttgag aaagaggaga cacctgagca ggatgatcaa aatagctaca
    23101 cctttagtag agtgactgct gctcatgatt tggtggtgaa actgcaagcg agccttcaac
    23161 cacccaaacc acagaaatca atccccatca atcagttgga aaaacgattg aacctattga
    23221 aataaaagga gaatacctat gtctaaacta cttgaattga aagaaaaacg taacgctgct
    23281 tgggctcaag cgaaagcctt tctggatact gttcgctctg aagacggctt ggtatcagat
    23341 gaagattcca aacgctatga agagatggaa gcaaaaatcg agctctacaa taaagaaatt
    23401 gctcgcttgg agcgccaaga aaagattgac cttgaactag cgcaaccgaa ctcacatgct
    23461 ctaacgacgc agccaacagt cattgtcgat aaccaaaaag aagatgaaaa gaaaggtgtg
    23521 gcatcaggca tctacaccca aactttctgg accagtatcc gtaagcgaca cttctatgac
    23581 gtgaaggatg ttcttcgtgt cggtgaagat acagaaggcg gacatcttgt ccctgatgag
    23641 tacgaaaaga aattggttca aggtcttcag gaagaaaact ttttccgtag tctcgcgact
    23701 gttattaaaa cctctagtgg tgagcgtaaa attccagttg taactggtca cggttcagcc
    23761 tcttggatgg acgagaatgg actctatcca gagacggatg agacttttgg tcaagtgacg
    23821 cttgattcgc ataagattgg gacagcaatc cgtatctctg aagaattgct caatgactct
    23881 gtctttgacc ttgagtctta tatgacttct gagttcgcac gtcgtattgg tacagaagaa
    23941 gaaaaatctt tcttggtagg tgatggttct aaaaaaccaa ctggtatctt tacgcaagca
    24001 gacgtagaag gaccaacgac cacaaccaaa gacatcacct ttgatgacat gattgagctt
    24061 tatcactcac ttccagctcc ttaccgtaag aatgcggtct ggattttaca tgacactacg
    24121 gtaaaagcta tccgcaagct taaggacaat aatggcaatt acatctggca accgtctaca
    24181 caggcaggtc aacctgattt gattctaaac cgtccttact acacgtcaac ctttgcgcca
    24241 cttccagaag caggaaacaa agctattgct tttggtgatt tctcttacta ctggattgcg
    24301 gaccgtcaag ggcgtacctt caaacgtctc aatgagcttt acgctaacaa tggtcagatt
    24361 ggttttcttg ccagccagcg tgtggatggg aaactcgttc taccagaagc ggtgaagact
    24421 ttgaccatca aaggtaaaac ggcatgatga cgttagaaga agtcaagctt tatctgaagg
    24481 tggaaaatga cgaggaggat tttcttatcc agcagttaat ggcaactagc caacagctct
    24541 gtggagatat tcttcgtgag gactccactt cagaagttct aaagacggca atcctctatg
    24601 gggttgccta tctttatgaa caccgtgaag aagccaatca caaagagtta aaggaaactc
    24661 tctaccattt gcttttagct gacaggaagg atgtgttctg atgaagattg cgcctctaag
    24721 agaacagctg gtctttcaag aaaagcgact caagcaggac gatatcggga acgagtcagc
    24781 catttgggat gaccttttta tgcgttggtg ttcttgtcgt cctcttgctt taacagagag
    24841 tgatgggagt gctaccaagc tcattcacaa caaggtacag tttacgcttc gatatgacaa
    24901 gaaggttctt gcccttaatt ctttaacgac tcggatttac ttccgtgacc agttttacgc
    24961 tatcgagtcc attgatggtg atactgtggc ccgaagcttg atttacatcg ttgcgactaa
    25021 ggaggagctt tatgactaag attggacttg atgatttaac ttctgtcatc gaaaaggagc
    25081 taacgactta tgccaaagag accatagaga ccatgcgtga ggtggttgag gaagtgacag
    25141 acgatgccgt tgatacctta accgttactt ccccaaaacg tcgtgggaag tatgccaagg
    25201 gctggaaaag caaggcaacc actgacacca ataccgctct gacgaaaacc attcacaacc
    25261 gaacaccagg actgacgcac ctccttgaat atgggcatgc taaacaaaat ggtggtcggg
    25321 ttgaagggcg aaagcacatc gctcctgtcg agaaaaaggc gattcagtcg tttgaagaca
    25381 aattgcgaca gaaactgtga ggtggcttat gagatttgaa gagctttttc ctgttctaaa
    25441 aaagaccaaa ctcccagtag cctatcacca ttttgaggaa gggcacagtc caagtccgcc
    25501 ttttatggtt tatctggtta cggattcaga caatcttggg gcagacaact gggcttatca
    25561 caaagctatc aacgtacaga ttgagcttta cacgactaag aaagatttag caacagaaaa
    25621 aacggtggaa tcagtccttg atgcccaccg tctttatttt gacaaggtag agacttacat
    25681 cactagtgag aaactctacc aaaccattta ttccatcaca ctattaggag gataaccatg
    25741 gcagaaaaaa acaaggtcac ctttggccta caagatgtcc actgggcaga agtcaccagt
    25801 gagggagctg acggggcttt gacttatggc actgtcgagc gacttcgtgg tgctgcagaa
    25861 ctaacccttg aacccactgg agacaagggg tcttacaagg cagacaacat caacttttac
    25921 acgactgaat ctaatgacgg ctatgaggga acactgaaag ttgccctctt atcacaggag
    25981 tttctgactc gtgttctagg tgagaaattg gatgcgacca ccaataccat ttcagagatt
    26041 gccagtagca agaaaaagaa cttcgctctc atgttccgat ttgaagggga caaaaaagag
    26101 acgcttcatg tgctttatta ctgttatgcg tctcgcccaa ctgttggctc aaaaaccaag
    26161 tctgggtctg atatcaatga ggtagagttg acctttacgg caagcccacg accacttgat
    26221 aaggtggtga gacgcagaac aacggaagaa accagtgatg agattcgtga gaactggttt
    26281 aagtcggtct ttgaaccagc tgcttaaagg aggagaacat gcgacaaaat atcacgatta
    26341 gtggaaaaac atatcccctg gccactaacg cctatacacc gattgcttat aaagagcaat
    26401 tcggaaaaga ttatttccaa gacctcttta acatgttgag tgcggaatcc atcatggctc
    26461 agcttgagca attggaagaa ggggaagagt taaaggctag tcagattgac ttgtctattt
    26521 tatctgactt cgacatgacc tttttccacc gtctcttttg ggtctttgcc aagtcagcca
    26581 atcctcgaat caagcccttc gaggatttct ttatgtcgat ggaggaattc cctcttcagg
    26641 aagttggccc cgtcttaatg tccatgctta accaagggat gacaaccaga aaaaagcaga
    26701 aacttccgaa acagcgagcg aggaagtctt cacggtagaa agctacctct cttgttgtaa
    26761 ggagactggc ctttccattg acgatttaaa gcatatcaca attggcatgg cacttgacta
    26821 tcagacggat tatgtggagc taagaagtcg agatgagact ggagttcgca gagcaaccca
    26881 agcagacctt gacaattttt gataggaact gaacacgggc taaaagctgt gtaaaaaaga
    26941 gagatggact ttagtgcaag caccattctt ccatctccta tttttacttt gcttttgacg
    27001 cccttagtat cttgatggag ggaggtgtga cgatggcagg aaacattaag gggattacca
    27061 ttgagattgg tggcgatacc cagcccttac aaactgcctt aaagggtttg aataaacagg
    27121 cttctgaagc caccaaagaa ctgcgtcaga ttgacaaggc actcaagttt gacactggca
    27181 atgtcactct tttgacccag aagcaggaag tcttagccaa acaagttgag acgactaaag
    27241 agaaactcgc cacgcttcgt caagctcaag cccaagtcga ggctcagttc aaggccggta
    27301 acatcggtgc agaccaatac cgtgccttcc aacgtgaggt ggagagtact caaacagtct
    27361 taaagggcta tgaatcaaag ctagaaagtg ttaacagagc tctctcagaa aacggagcgc
    27421 aggttgaaac caatcagtcc aaacttaatc gtctccaaaa tgagcaggca cagttggtgt
    27481 cagaaagtga aaaactccaa agttccttta agctacaaga atcagcatta gcaactaccg
    27541 caagtgaggc cgataagtta gcacttgctc aacaaaaggt tgcttctcat tcagaaatcc
    27601 ttgagaaaca gatacataat ctggaacaac agctctcttt gacgaagagt gaatatgggg
    27661 agaattcagt tgaagctaac aagcttgaga aaacacttaa tgagaccaag acagcttaca
    27721 acaatctcca aaatgagatg gaggagttgg catctagctc tgcgagttcc aaagcttctt
    27781 tggaagaaac aaatagtctc ttaaaggctg atcttctcat ggagtttggt gaccaactgg
    27841 gagagttgtc gcagaagttg attgacttcg gtcaacaatc gcttgacgca tttcttgaag
    27901 ttgatgaggg catggatatc attgtcacga aaactggggc aactggttct gcccttgaag
    27961 agatgacaga catcgccaaa accctagcca ctgaactgcc aacagatttt aatacggcag
    28021 gaagtgccgt aggggagtta aatacgcaat ttgggttaac aggagatgcc cttaaatcag
    28081 cctctaccca gttaattcaa ttctcagaga tcaatgggag tgatgtgacg agttcagcca
    28141 tttcagccaa acaagcgatt gaggcctatg gacttgaagc gactgattta tcaagtgtct
    28201 tagacacggt tacttatacc agtcagtcaa ctggtgtcgg tgtccaagag ttgatggaca
    28261 aggcagtagc tggagcacca caaattaaag cccttggcct ttcttttgat gagggcgtca
    28321 ccttgatggg gcagtttgaa aaagcaggga ttgattcttc tgcagcactt tcgtcactct
    28381 caaaggcagc tgttaagtat gcgggtgatg ggttaacgct tcaagaagga cttgctggaa
    28441 ccattgaaca aattaaaacc tcaacgagtg aaacagaggc tctttctcta gcctcagaaa
    28501 tctttggtag taaagcagct ccacgtatgg ttgatgccat caagcgtggg gctttatctt
    28561 ttgaagattt agcaggaaca gctgataaag cagctgggat tgtcactcag acctatgagg
    28621 ggacgcttga tcctattgat cagtttacaa ctgctcaaaa cacggcgaag ttagcgatgg
    28681 ctgaaatggg agacgctatt gctgcaaccc tagctcctat cttggaaata ttagcgagtt
    28741 tacttcaagc tgttgccaca tggttctctg gtctttcaga gccagtcaag cagtttattg
    28801 tcattgtcgg aagtttggtt gcagcccttg gcttagtcct cccgattttc attgccctgc
    28861 aagcagctgc tatggcaatg ggaactacca tcatggggat gataactgca gcagctccaa
    28921 ttgtagggat tattcttggt gttattgccg ttgttgcctt actggttgtt gggattcaac
    28981 aactctggca acatcacgaa gggtttagaa cagctgtgac agaaatctgg aacgccatct
    29041 atgccttttt atctgtcatt atccaacaga tatcaagctt tgtcatgtcg atatggggaa
    29101 ccttgactac ttggtggaca gagaaccaac agctaattct taatgccgca aacactgtgt
    29161 ggacagctat ttcaacagtc attcaaacca tcatgaccat tcttggacct taccttcaag
    29221 ccagttggga gaatatcaaa ctgattatca cgacagcttg ggacatcatt aaagtggtcg
    29281 ttgaaacagc tatcaatgtt gtcttaggta ttatcaaggc agttatgcag attatcacgg
    29341 gtgattggtc aggtgcctgg gaaaccatca agcaggtcgt ctctacagtt tgggaagcca
    29401 tcaagtcact gatttcgatt gttctaagtg ccattgctca gttcatttcc aattcctgga
    29461 atggcattaa aggcaccatg acaaacttac taaatagtat caaatctgtc gttagtaatg
    29521 tctggaatag cattaagtcc acgattagtt ccattctatc tagtattggc tcaacggtat
    29581 cttccgtttg gaacggaatg aaggctacta tctcaggtgt cctaagtggt atttcaaaca
    29641 cagtgtcctc tgtctggaat ggggtcaaat cgaccattac aaatgccatc aatggggcaa
    29701 aaaatgcggt ctcttcagcc atcaatgcca ttaagaatct ctttaacttc aagattaagt
    29761 ggccacatat tcctcttccg cactttagtg tgtcaggttc tgccaatccc cttgattggt
    29821 taaaaggtgg cttacctaag atttccattc agtggtacgc caagggtggg attctgacta
    29881 aaccaacggc atttggtatg acagggaaca gcttgatggt tggaggagaa gcaggacgtg
    29941 aagcagttct acctcttaat aaccaaactc ttggcagtat cggacgcagc atcgcagcca
    30001 ccatgcctag caagggaaca accataacgg tcaatatcac agatgttgtg attcgtgaag
    30061 aagcggatat gaaaaaacta gccgattatg tagctggtcg attagctgat gaaatggcac
    30121 gacaagcctt actgagagga ggaacagtgt gattaaacat aatgaattag tactgaatgg
    30181 aaaaggcacc tcgtcttttc cttttaaagt tcttgtggaa gatagaccga gtattcaagt
    30241 gccacggtct aaaacgcaac tcctagacca tcgtgggttg agtggggcga ttgttcaaac
    30301 caataagcat cgtgaagtga ttgagaaatc ttaccgcttg tatctgattg gtgcgagtga
    30361 gaaagaggtc aatgagttct cggcttatct catgcaggaa ggattttggc tagaaagcga
    30421 acgccttaag ctcactaggc tctggtgtta ccgaacggat agctttgaca tcaaacagga
    30481 tgaccatgat gtgtatgtga ttgatgtgac ctttatctgt caccccactc gcttttttaa
    30541 gagtggggat aggcaagttt tgagtgccaa tggtgtgtta aaaacacaag gctctgccct
    30601 tgcctttcct agcattacca taactggtca atccgtatca gagacttcgt ttacagtagg
    30661 tgaccaggtg atacggattg agaaatttac agagcctctt gttatggtga atcacccaga
    30721 ccgtcctagt tttaagaccc tatcagggaa agctgtcaag tggtctgggg acttcatcac
    30781 gattgatgct agtcatccaa cacaatctgt cggagtgatt ttaggcagtg ggatcttatc
    30841 gcttactttt gaaacgaatt gggggtgggt gtgatgcttt acttacttga tggtcaaaca
    30901 aggacagcga agtggaacgg ccagcccctc tttgaagcag taagtgcgac ggtagaagaa
    30961 aagttgaatg gcacctttca gcttcgctta acttatccca ttacagattc aggtgttcat
    31021 gaaaccctta gaacagatga attgattttg tgtccaactc cagatttagg aaaacagctc
    31081 tttcgtgtta agcaggcaaa aattcaagac gatacggtag agcttgagtg ctctcacatt
    31141 tctgatgatg tgatgaaacg tcaaattaag cccttttctg cgactaatac cacctgccaa
    31201 tcagccctta tgaggatggt tgaggtttgt ccatctgatt taggactttt tagctttgac
    31261 agtgatgtga cggagcgtca tacctatgtg tctgacgaag acttgacact ttatcaagct
    31321 ctaatggatg gtaaacattc catccttgga acctgggaag gtgagctcgt tcgtgataac
    31381 tttcagctga tagttaagaa acaccgtggc aacgataagg gagttattct cacaagccat
    31441 cacaatctga aagcttttga ggataagggt gattctgaga aggtcattac gcgcatctat
    31501 gcgacctcaa cctttcagat agaaggtagt gatgaggcta ccgttctttc tgtcgttgtg
    31561 gaaagtcccc tcattaacca atacccttat atctatgaag cacggtatga gaataacacg
    31621 cttcagacag aggaagaatt acgccaatgg gtgatggcta agttcacaca tgagcacatc
    31681 gatcacatct ctagacagat aaccgttgaa gcttatcagc ttgatggtca agaagtccat
    31741 cttggagata cagtcactct taaaagtcaa aagcacaagg tagatgttaa gaaaaaggca
    31801 gttggttata cctttgacgc tctagaagaa gtgtatcttt cggtgacctt tgatgatgag
    31861 gttagcttta caacctctgg atcatctggg ccccattcgc taaccagtgc ggctaagacc
    31921 attcttgatg tccatcagtc ggttacagaa caccgtgcgt ctaaggagcg agctaatttt
    31981 aacaaggtct ttgataggca ctttgagcgt cttcaaacag aagttgaaga tggtatcgct
    32041 aaggccgaag cagaaggcga gcgttctggg aagaaagctg cccttgatta tctggcaacg
    32101 gatgcccttg aagcacgagt cgcagccctt caaaaagcta cgattgatga gttgaccgtc
    32161 tctagttcag catggatgac aaggcttgtc tcccaacaga ttctatcaga gtatgtgaag
    32221 agtttagagg tcgaggcaga cagggtcgtt attccaggtc aacatacccc agtctttagt
    32281 ttggataggg atgggaatct ttccattgat acgccactct taaaggtgag aggggaaagt
    32341 atagtgacac aagctgatct taaaaccatc tctttaactc ctggaccaaa gggtgatgct
    32401 ggagcagatg gggtcggtat tcaatcaagg gagcagtatt acttagtttc tgcacaaaag
    32461 actggtctta cgacaacaag cactggttgg agtaaaacga ttccttctct aacttcaacc
    32521 cttaagtatt tgtggaatta tgaaaaaacc acgttcacca atggctcaac gacagtcaca
    32581 acgcctgttg tcatcggtgc ttatggggac aagggtgtgg atggaaaggc tggtaaggat
    32641 ggaaagaccc tatacacttg gcgcatgtac gcagatagtg acaagggaga tgggatttct
    32701 gccgtctcaa cgggcaaacg ttacctcgga ctagcagtta atagggagag cgcaacgcct
    32761 tcaaccaatc ctagtgacta tacctggtcg tccttttttg aaggaacaga actcggtggg
    32821 cgcaattaca ttgacgatta tgccatgaaa gcgatgactt tttcatctgt tacctctgag
    32881 tggaagaagg aggtaattga agatacgagt tcagttagtg gcgtgactgt gaagatgacc
    32941 tgtaccaaag caggtactgg cggctttcat cggaacttcc atgatttacg aagtcgagtt
    33001 ggtgctaaca tgacgtttag tattgatctc aaggcaagca agacggttag tcttaccatc
    33061 ggaaatgaac tgagcggtac taaggttgtt caagttagtt ctagctggca gcggtattcg
    33121 gtgtcttgga aagttactag tgcccagaat cattcctatg tcttttacct gaagtcagga
    33181 acatgggtgg tgggagatgt ggtttatctt cgaaatgttc aactagaaga tggcaacgtt
    33241 gcttcagctc caggaccatc tttgaatgat cttatcgccc agattgatgc caaggcagat
    33301 aatggcttca tgaagcagca attagacctt ctaactgaaa aaacagaatc tctccgagtg
    33361 gatcttgaag caagagcgtt ggcaaaagaa gtggccgatt ggctcaagtc ttataaagaa
    33421 tttgagaaaa ataatgaagc cgtccttgcg caatttaatc aagactttat tgataataca
    33481 gctcgtatcg cagcgattga agccgatctt aaagccaaca gtctcttgct taactttgtg
    33541 aatacttatc tgagagctgg tgataatggg gtcattattg gtaagaagga taactctgag
    33601 gttatcgaat taaccccaca agggatgatg attaagtcag ctggtaatgc cgtcatgacg
    33661 gtgacagctg gtgtcattaa gattcatcat ggggtatttg tggagacctt acaggttggt
    33721 tattatcgac tagaagccgc aaggcataat gctaagcatt tggtttgtcg ttttattgat
    33781 gccaagtaga aaggagactt tatggcagat tatggttcaa ataatgatag gggctatacc
    33841 ctacttttgc gagtggaaga aacaggcact tcgaccgcta ataacacatc tactgttcga
    33901 gtccaactct ggctaaagaa tggttatacg acctttggga tgtatgactg tagggcaagt
    33961 gtgactatca atgggcagac actttcttgg tcagggcgac cagatatgta cacggctcat
    34021 agttcccttc acttgattga taagaccatt actgtgccgc atgattctaa tgggtcaaaa
    34081 accatcagtt tttctgcgac cttctcagga tctggtggtt ggtcacctgg caccttaaat
    34141 acagggtcac aaacgctacg cttaagtgat atcccacggt catcgagtgc tacagtttct
    34201 gggaatatga tgggacaagc cgtaaccata acgattaaac gtgccagtag tgattttacc
    34261 cataacatga cctggcattt tggcagtcta agcggcacga ttggaacagg cattgcgacc
    34321 tctgtaacgt ggatgccttc gatttctcaa ctagcaactc aaataccaaa tagcacctca
    34381 ggtaatgggc atttaacgct ggccactatc tatggtggta agacaatagg ttctatgaca
    34441 attcccatta ccctcaacct accgacgtca gttgttccaa ccttgggcag tatttctatt
    34501 tcagaatccc atgccactgc aaaaacgatt ttaactggca ccagttttgc ccagttggtg
    34561 tccaatccta aagtgacctt caatcaagga gcgggtattt acggatcgac gattccttcg
    34621 acgggttttc gcgcagaggt ctttaagttt gagaacaatc agtgggttca acttcaaaat
    34681 gtcactagta gtaataacgg actattgggt ggaataaact ggattggtcg agctaaggtc
    34741 tctgcctatg tgaccgattc gagagggcga caaagtgctc gaaaagaagt ggagataacc
    34801 ctattagagt atttcaagcc tatcttttca ttctcagcgg ttcgagctgg ttctagtatg
    34861 aatcaggtga cggtcatacg aaagcttaag attgcccctt taaccatcaa tagtgtccaa
    34921 aagaacaagg cggccttgac atgggaagta gtcgatttag caagcggtca gaaggtcaca
    34981 aacgctggtg gtgccgccaa ctggacgtca actacggaac acacaaagac cgatttccaa
    35041 gctattttag gtggcaccta tgataccacc aaatcctaca ccattattgg aaggctagaa
    35101 gatctctttt actccacgac ctttgaattt accattggtc cagaaaaagt tgtctacggc
    35161 ttaagttcat ctggtatggg gatagggaaa gcctggacaa gaggcgtgct cgatgtggat
    35221 gggagcttac ctgcttattt tgacggggac atttatatga agaataagaa acttcttgat
    35281 attttctatc cagtgggtgt tatttacgag tcaacttcaa gctctaatcc ctcaacattt
    35341 atgggaggca cttgggagcg gtttggcaac ggtcggatct tggttggtgt gtctgagaat
    35401 gaaagtgagt ttaatagcgt caatcagtca ggtggtagta agacacatac cttgaccatt
    35461 gatgaaatgc catctcactc acacgctcag tatgtgacgg ctaacaatgg ttcaggagct
    35521 gttcgtcgtg actactcttc agatggcagc tctacgattt atccgcaagg aaataacaca
    35581 ggaaatactg gtggcggtaa gccacacaat aacttacaac cttacgtcac ggtttaccgt
    35641 tggcgtaggg cagcataaga aaggaaagtg tcattatgaa agaattactg gcaacaaata
    35701 aagtcctctt ctcagcgatt ggaggactta tcggatcaat ctttggagaa gtcgatggct
    35761 ttctctttgc attgatggtt tttatttcca tcgattacat cacaggtctc atggcagcag
    35821 ccgttgagaa gaaattagct agcaatattg ggtttaaggg catcttcaaa aaaattgtcc
    35881 ttttattttt ggtagcagta ggacaaatca ttgatgagca tgtcttgaaa caagggggca
    35941 tggttcgtac tgccatcatc ttttactatc tctcaaatga aggcttgtcc atcattgaga
    36001 atgcggctag gattggacta ccagttcctg agaagttgaa agatgtgctg aaacaattga
    36061 aacaaggaga ttaggatgac ttttttatca aagattaaag acggttgttt agcatcttgg
    36121 gagcatggta ttcttccttc cgtgtcagca gcacaagcta tcttagagag tggttgggga
    36181 gagtctttgt tagcacaata ccctaatcat aatctctttg gaatcaaagc tagttctgat
    36241 tggaagggaa aacgtgttga ccttccaact caagaataca ttgatgggaa attcgtcacg
    36301 gttgaggcta cctttagaaa gtatgactca tgggaggaat ccattaaaga tcatgcgttg
    36361 ttcttttcag aaacagcatg gcgacgtagc cattaccaaa acgtgctcgg tgaagaagac
    36421 tataaaaaaa cctgtctcgc tttacaagcg tcaggttatg cgacagatcc taactatggg
    36481 tcaaaattga ttacacttat tgaagcacat catctcaaca cttgggatga ccgtatttta
    36541 aacaaaaaag gagaaacgac tatgagtaaa catttagtta tctgcggaca tggacaagga
    36601 caaacaggtt atgatcctgg agcaacgaat cctagtttag gtattactga agcaggaaag
    36661 gttcgtgagt ttgcgaatct gatgaagaag tattctggga atcggattga ttacatcact
    36721 gaccataatg tctataacta ccgcagtatt ggtagtttgg gaaatggcta tgagtcgatt
    36781 acggaacttc atttcaacgc ttttaatgga caagcgagag gaagtgaaat tctgatttat
    36841 tcaggctaca cagctgatag tttagaccaa aaactgctag ctattctagc caagcgtttt
    36901 acgaatcgtg gttttaaaca agtgaactgg ctctacaatg ccaatgtctc agcaagtcga
    36961 ggatacaatt atcgattggt ggagattgcc ttcatcgata ataatagtga tgtgggcatc
    37021 tatgaagcca ataaagacag tatggctcgt gaatttgtac aagccatcac tgggcaagct
    37081 caagtaattt cacccagtcc caatactcct caatcaaggg ttacttccta tcatgttggt
    37141 gaccctgtta ctgttcaaca acatgcgacg cattaccaga caggtcaagc gatttcgtca
    37201 tgggttaaag gcaaaacttt caaggttatt cgtgtgaagg atgttaatca atccaatagt
    37261 aagaaagcct acctgcttga gggaattaac tcatgggtct tggaacaaga tgtcaaaggc
    37321 acaacgaacg gacatagtga acagacttat acggttcaaa agggtgatac cctttatggt
    37381 attgcacgga agtttaagac cagtgtcagt gaattggtgc gacttaacag catcatcaat
    37441 ccaagtctga tctcagttgg acagaagttg aaactaaaat aagctacaca ataggcagct
    37501 ttaatggtat actattaagt gtagaaaagt gaagacggtg gttcctaaat cctgaaagag
    37561 agaggtgatg cctatgggca attcatcaaa atctgatgga aaggagggag ctttcttttg
    37621 accgcttttg aagtcgtgca aatcatcatt ggttttggta gttttaccgt tgctttgatt
    37681 ggcttgtgct ataaaatctt caaagatgac gacaaaaaga aataacccgt ccccactttt
    37741 gaccgagttg ttggacgagt tatagtctaa ttaaacgagt caccgtcttt ttaacggtcc
    37801 tacattgggg ggtggattgc cgtccagctc ctttttataa gttcattata acatacttgt
    37861 atctcagcgt gttgaaatag ccacgctttt tctttttgct tttttcaaaa actgcggaaa
    37921 atttgtttcc aaatttactt agtaatatgg agggagtagg tttgcgaaaa gcttgactta
    37981 tcttcccttt agagtgatat atggtgtaca aaagttatag gaaggagcct gaatggcagt
    38041 aaggttaatt aagacaaaaa gtaatcgtaa aaaacaacgt gtctgtgctt atactagggt
    38101 gtcaacaaca aatagtagtc agcttgattc ccttgaaaac cagaaggctt attttgaaac
    38161 attctatgtc aatcgggagg acgtcgattt tctcggagtc tattacgata aggggatttc
    38221 tgggtctaag gagaaacgac cgagttttca agctatgctt gaagcttgta ggcaaggtca
    38281 gattgatttg atacatacca agtccatttc acggtttgcg aggaatacca tgacggtact
    38341 tgaagtcagt cgtgagttga aagccctagg tgttggtatc tattttgaag agcagaacat
    38401 caatacctta tccaatgaag gagagggcat gctttcagtt cttgccagtt tggcagagga
    38461 agagttgcaa agcatgagtg acaaccagcg gtgggccttt cagagaaagt ttcaacgtgg
    38521 ggagatggtc atcaacacca agcgattcat gggttatgat gtggacgata aaggagagtt
    38581 ggtcatcaat gaagcagaag cacagattgt gagacggatt ttccaacttt atcttgatgg
    38641 tatggggatg catcgaatag ctaaactttt aaatcaagag aatgtgccta cggtgacaga
    38701 tgctaaatgg catgatacaa ctgttcgaaa tatcctaaag aatgaaaagt acaaaggaac
    38761 tgtccttctt caaaaatatt ttcatgttgg tatcaattgg cctaagagat taaaccaagg
    38821 tcaggtagag caatatttga ttgaagataa ccatgagcca attgtttcaa aagaagtctg
    38881 gcaggctgtc caagataagc ttgcaagcaa aacttggaaa cagggagtca ataagcacta
    38941 tcgatttacg agtatgctta aatgcgagta ttgtgggtca accctcaaac gccaggttac
    39001 ctacaagaaa caaatagttt ggtgttgttc caagtatatt aaagaaggta aggcttcctg
    39061 ccgaggcatg cgcgtgcctg agaaagctat tgaagagtgg aaacttagga cacctgtaac
    39121 agtgatagaa aggaacgaat atgggcaaaa acattacagt tattccagcc aagaaagtgc
    39181 agctgacggt cacccatcag caagcaacca aaataagagt ggcagcctat tgtcgggtgt
    39241 ccaccgacca agacgaacag ctatcaagct atgagaacca agtatcttac tatcgtgatt
    39301 acatcttaaa acatgaagat tatgagcttg tggacatcta tgcggacgaa ggcatttctg
    39361 caaccaatac caaaaaacga gaagccttta accgactgat acaggattgt agagcaggta
    39421 aggtggatag gattttagtt aagtccatta gtcgttttgc cagaaacacc ttggattgta
    39481 tcaagtatgt ccgtgagctg aaagaattgg ggattggtgt cacttttgaa aaggagaata
    39541 ttgatagcct tgactctaaa ggtgaggtat tactcacgat tctttcttct cttgcccaag
    39601 atgaatcacg ctccatttca gagaatgcga catggggcat tcgtaagaag tttgaacgtg
    39661 gagaggttcg tgtcaatacc accaagttca tggggtatga taaagatgag aatggtaagc
    39721 tagtcattaa tccagagcaa gcagaggtag ttaaacatat ctatcaacag ttcctaaaag
    39781 gctacagtcc agaaagcatt gccaaggaac ttaacactaa cggtatcaag ggatggtcag
    39841 gaaaagccaa ctggtatcct agcagtattc tcaaaatgct tcagaatgag aagtacaaag
    39901 gtgatgcctt acttcagaaa acctacacgg tggatttcct tacaaaaaag cgaacagaga
    39961 atgatgggca ggtcaatcaa tactatgtga ccaataatca tgaagccatt attgatacag
    40021 aaatgtggga cactgttcaa gaagaattgg caaggcgaaa agacttcaga gaaaagcaca
    40081 agctgaaaac ctatatcatg caaagtgacg acaatccttt taccactaag gtcttctgtg
    40141 ccgagtgtgg ttctgccttt ggacgaaaga actggacgac cagtcgaggt aaacgcaagg
    40201 tttggcagtg taacaaccgc tacaaggtca agggacagat tggttgccag aacaaccata
    40261 ttgatgaaga aatgcttgag aaagccttca tgaaggcagt ggaattgctt caggaacata
    40321 gagcagatgt ggtggttaag tggcaaaagt tagaaaaggg aactaatctg ctgcataagc
    40381 actatgccaa acaaatgtat cagttactag atttagagaa atttgatgga actatcatga
    40441 acaaagtttt agatcacatc agtatttcag aagtcggaca aattatcgtg attttcctag
    40501 aaggaactga agtagaatta taagagactg tgactgagag gatgcagtct ttttgtggtt
    40561 ttagtggtat aattacttaa ggagtataaa gcatgggtga aaatttgaat tctaatatag
    40621 atcctcgaat tatatttaaa caattacctc cagctacaat aatcaaaggt tttgtagctg
    40681 gtgagaccaa atgtaattat gaaatttatc tattggaatt gcttaataaa tcagtatatt
    40741 ttcgggaaaa aggtaaatct aaatttcacg cacctgaaga cgagagccac ggggaatgtg
    40801 atgctgtagc agatgattac aagatagact tcaagcttct ttcttcaagt agtagactgc
    40861 aggcaagcag tttattttca cctagtatca ctaatcttgg aaatgggatt actgcaattg
    40921 gagaaagtag aaatccgaac ggggaaataa aagcaactca gattcatgct gcgtttcgat
    40981 ttagaactgt atctgattta atacgactca aggtaaagcg tcaacatgtt aggaaacaat
    41041 gccttgaaaa agatattata aaagtcctaa atatgcttga gaagcagaaa aatctccttt
    41101 tatttttccc ttacattttt tatacggaag aagaaattag cactaatgaa ctagatgata
    41161 tcattttaaa tgcaataagc tatgattttt cttctctttt cgcctacagg cacataaagg
    41221 tacaatcgtt tgaaacttat ttacttacta ttgtacgtga tgagtttaat atttttaaga
    41281 tatccaacaa aaggctaaaa ctcattgaaa aactcagttg ttcggaatta cctacttttg
    41341 ttaaattaaa aagttattct atctaaaagg ggaaatgttt tggttaatcc agagttttat
    41401 gaaaaaatag ctaaaatatt ctgtggagat gatttagaac tattcaagta caaatccggc
    41461 ccagacctag ttcacttttt taataataat ttcaaaatat ctgatagcta tggacagggc
    41521 tttccaacaa ggtggagata tgtaaatcaa aaattattag atttttcttc agtaggaaag
    41581 attgatgagt atttcaatat aattctttct aaacagtatc ttttaacaga aagacaaatc
    41641 agtgaaattg atgcacttgt gcatcaacaa agaatattag atgaactgaa taaagtatgc
    41701 tcggtttact cgctaaaact atctcaaaaa gatggtgcat ttcatttagt agaaattgat
    41761 ttagatctga ttgaaattgg tagtggtggt tttgcaaata tttatttcca aaaatcaaca
    41821 ggactagtat tgaaaaaact taatgaggaa tctgcaagaa gcgcttcaat ccgaagccgc
    41881 tttaagcgtg aatatgaaat aactaaatct tgttcagata ttggaagtat cattaaagtg
    41941 tatgattttg a
//
LOCUS       DATRNK010000010        38416 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.010, whole genome shotgun sequence.
ACCESSION   DATRNK010000010 DATRNK010000000
VERSION     DATRNK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38416
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.010"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(23..484)
                     /locus_tag="VBC52_000558"
     CDS             complement(23..484)
                     /locus_tag="VBC52_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6882162.1"
                     /translation="MKNRDFRVREQLETFGVRVSALIIGNQKLLLIYAPHLDKYYLPG
                     GALQVGEDSNKAVAREVLEEIGLHSQVGDLAYIVENQFNIKRHHYHSVEFLYFVNLLG
                     QAPESIKEGTHKRHFVWLPIKELTKIDCNPNFLAQDLIEWPGHVVHKIVQN"
     gene            complement(474..956)
                     /locus_tag="VBC52_000559"
     CDS             complement(474..956)
                     /locus_tag="VBC52_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO6882163.1"
                     /translation="MTKLATICYIDNGKELLLLHRNKKENDVHEGKWISVGGKLEAGE
                     TPDECAKREILEETHLTVKKMDFKGVITFPEFTPGHDWYTYVFKVTDYEGELISDDES
                     REGTLEWVPYDQVLSKPTWQGDYEIFKWILEDVPFFSAKFVYDEHQNLIEKTVNFYEK
                     "
     gene            1174..4392
                     /gene="hylB"
                     /locus_tag="VBC52_000560"
     CDS             1174..4392
                     /gene="hylB"
                     /locus_tag="VBC52_000560"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_192884539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEO6882164.1"
                     /translation="MEIKKKHRIMLYSALILGTILVNNSYQAKAEELTKTTSTSQIRD
                     TQTNNIEVPQTESTTVKETSTTTTQQDLSNPTASTTTATATPSTMKQVVDNQTQNKEL
                     VKNGDFNQTNPVSGSWSHTSAREWSAWIDKGNTADKSPIIQRTEQGQVSLSSDKGFRG
                     AVTQKVNIDPTKKYEVKFDIETSNKAGQAFLRIMEKKDNNTRLWLSEMTSGTTNKHTL
                     TKIYNPKLDVSEVTLELYYEKGTGSVTFDNISMKAKGPKDAEHPQPVTTQIEESVNTA
                     LNKNYVFNKADYQYTLTNPSLGKIVGGILYPNATGSTTVKISDKSGKIIKEVPLSVTA
                     STEDKFTKLLDKWNDVTIGNHVYDTNDSNMQKLNQKLDETNAKNIEAIKLDSNRTFLW
                     EDLKDLNNSAQLTATYRRLEDLAKQITNPHSTIYKNEKAIRTVKESLAWLHQNFYNVN
                     KDIEGSANWWDFEIGVPRSITGTLALMNNYFTDAEIKTYTDPIEHFVPDAGYFRKTLV
                     NPFKALGGNLVDMGRVKIIEGLLRKDNTIIEKTSHSLKNLFTTATKAEGFYADGSYID
                     HTNVAYTGAYGNVLIDGLTQLLPIIQETDYKISNQELDMVYKWINQSFLPLIVKGELM
                     DMSRGRSISREAASSHAAAVEVLRGFLRLANISNEERNLDLKSTIKTIITSNKFYNVF
                     NNLKSYSDIANMNKLLNDSTVATKPLKSNLSTFNSMDRLAYYNAEKDFGFALSLHSKR
                     TLNYEGMNDENTRGWYTGDGMFYLYNSDQSHYSNHFWPTVNPYKMAGTTEKDAKREDT
                     TKEFMSKHSKDAKEKTGQVTGTSDFVGSVKLNDHFALAAMDFTNWDRTLTAQKGWVIL
                     NDKIVFLGSNIKNTNGIGNVSTTIDQRKDDSKTPYTTYVNGKTIDLKQASSQQFTDTK
                     SVFLESKEPGRNIGYIFFKNSTIDIERKEQTGTWNSINRTSKNTSIVSNPFITISQKH
                     DNKDDSYGYMMVPNIDRTSFDKLANSKEVELLENSSKQQVIYDKNSQTWTVIKHDNQE
                     SLINNQFKMNKAGLYLVQKVGNDYQNVYYQPQTMTKTDQLAI"
     gene            complement(4444..5490)
                     /gene="rfbB"
                     /locus_tag="VBC52_000561"
     CDS             complement(4444..5490)
                     /gene="rfbB"
                     /locus_tag="VBC52_000561"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEO6882165.1"
                     /translation="MTEFKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGN
                     RANIEAILGDRVELVVGDIADAELVDKLAAKADAIVHYAAESHNDNSLEDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGNGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLY
                     GEGKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKD
                     AYDHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKD
                     AVEAKYAKTQEVIK"
     gene            complement(5697..6290)
                     /locus_tag="VBC52_000562"
     CDS             complement(5697..6290)
                     /locus_tag="VBC52_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEO6882166.1"
                     /translation="MTEQFFDKELTCRPIEAIPGLLEFDIPVRGDNRGWFKENFQKEK
                     MIPLGFPESFFEADKLQNNISFNKKNTLRGLHAEPWDKYVSIADEGRVIGTWVDLREG
                     DSFGNVYQTIIDASKGIFVPRGVANGFQVLSDKAAYTYLVNDYWALELKPKYAFVNYA
                     DSNLGIQWENLEEAEVSEADKNHPLLKDVKPLKKEDL"
     gene            complement(6290..7159)
                     /gene="rfbA"
                     /locus_tag="VBC52_000563"
     CDS             complement(6290..7159)
                     /gene="rfbA"
                     /locus_tag="VBC52_000563"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEO6882167.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSVLMLA
                     GIKEILIISTPQDLPRFEDMLGDGSELGISLSYAEQPSPDGLAQAFIIGEDFIGDDHV
                     ALVLGDNIYHGPGLSAMLQRAASKESGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PAQPKSNYAVTGLYFYDNDVVEIAKNIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRMQNVQVANLEEIAYRMGYITREQVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            complement(7218..8321)
                     /locus_tag="VBC52_000564"
     CDS             complement(7218..8321)
                     /locus_tag="VBC52_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEO6882168.1"
                     /translation="MKKIAIIGAGAVGATLAYYLSKEKDIQVTVFDYGVGQATKAAAG
                     IISPWFSKRRNKAWYRMARLGADFYSKLVTDLQKDGFETKFYQQTGVFLLKKDESQLE
                     SLFALADKRRLESPLIGDLQILNKSEANTHFPELDGYEQLLYASGGARVEGADLTRIL
                     LEASGVNVIKDEVHFTITDNGFRVQGIDFDKLVLASGAWLAKILDEHNYQVDVRPQKG
                     QLRDYYFSNINTGKYPVVMPEGELDIIPFDNGKVSIGASHENDMAFDLSIDFKVLDKF
                     EEQAIGYFPQLKKADTTSERVGIRAYTSDFSPFFGPVPCMEGAYAASGLGSTGLTVGP
                     LIGYELCQLILNKENQLNLEDYDITKYVKKISR"
     gene            complement(8330..9118)
                     /locus_tag="VBC52_000565"
     CDS             complement(8330..9118)
                     /locus_tag="VBC52_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEO6882169.1"
                     /translation="MKARELIDVYETYCPQELSMEGDISGLQIGSLDKEIKTVMVALD
                     VRETTVAEAIERQVDLLIVKHAPIFRPLKDLVATPQNKIYIDLLKSDIAVYVSHTNID
                     IVPNGLNDWFCELLDIQYPDILSETSNGYGIGRIGDVRPQSFEFFAWKIKDVFGLDSV
                     RLVSYDKSNPEIQRVAICGGSGQSFYKEAIAKGADVFVTGDIYYHTAQEMITNGLLAI
                     DPGHHIEVLFVSKIATMIEQWKLEKDWDISVLESKAPTNPFYHM"
     gene            complement(9108..9791)
                     /locus_tag="VBC52_000566"
     CDS             complement(9108..9791)
                     /locus_tag="VBC52_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEO6882170.1"
                     /translation="MDLQLSKRLQKVANYVPKGARLLDVGSDHAYLPIFLLQMGYCDF
                     AIAGEVVNGPYQSALKNVSEHGLTSKIDVRLANGLSAFEEADNIDTITICGMGGRLIA
                     DILNNDIDKLQYVKTLVLQPNNREDDLRKWLAANDFEIVAEDILTENDKCYEILVVKH
                     GHMNLTAKELRFGPFLLSNNTTVFKEKWQNELNKLTFALNSIPNSKIEERAILEDKIQ
                     DIKEVLDES"
     gene            complement(9895..10575)
                     /locus_tag="VBC52_000567"
     CDS             complement(9895..10575)
                     /locus_tag="VBC52_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEO6882171.1"
                     /translation="MTYLEQYQSGQLTLPSALFFHFKSIFKTADDFLVWQFFYLQNTT
                     NLSDLTPSRIATSLDKTVADINRSISNLTSQGLLDVKTIELNHEIEIIFDTSPVFAKL
                     DKLFEEDNQVIIDNKTSDSNRLKDLVGDFERELGRLLSPFELEDLQKTLQEDQTDPDI
                     VRAALREAVFNGKTSWNYINAILRNWRREGLTTLRQIEERKQAREDNQMKDLAISDDF
                     KNAMNLWE"
     gene            complement(10693..11211)
                     /locus_tag="VBC52_000568"
     CDS             complement(10693..11211)
                     /locus_tag="VBC52_000568"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEO6882172.1"
                     /translation="MDLNNYIASIENYPQEGITFRDISPLMADGKAYSYAVREIVQYA
                     ADKDIDMIVGPEARGFIVGCPVAYALGIGFAPVRKPGKLPREVISADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVVAGCAFLVELDGLNGRKAIE
                     GYDTKVLMNFPG"
     gene            complement(11334..13898)
                     /locus_tag="VBC52_000569"
     CDS             complement(11334..13898)
                     /locus_tag="VBC52_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GBS Bsp-like repeat-containing protein"
                     /protein_id="HEO6882173.1"
                     /translation="MRPIVRKKMYKKGKFWVIAGIVTILGGSAIIGQDVKAEQAEAVT
                     STISEKIDSSQTISDTSKLTLPVNSSEAMKNSAEPLIKTGFATSVSSNPREIAATPVK
                     TFDASSKVVVKASTAEHSANQTNSNVNQVANDSEVITQQNSTKQLPTVTYSAHVQDIG
                     WQKSVDNATVSGTVGQEKQVEAIKLSIKAPEGITGKLSYKTYVKGQGWQSSVESGQVS
                     GTVGQSKPIEALSINLTDNLQKLYDVYYRVHVQDIGWMAWAKNGAYAGTLEMSKRLEA
                     YEVKFTLKGQSVLTPTILKEERPVLNYQVKVGQNGWQSNKLEGQMAGTLGESKALDGV
                     KFTLSTLKYGDILYRTHVQDKGWGEILGSQTSKDYQGKRLEAIQLDLSGNLKQNYDIY
                     YRAHVQDKGWMAWQKNNSVAGTVGESKRLEALEVKLVAKASDFSSQTDHSFLEMKHPG
                     LSYQTYLQKDGWKPTVLEGQLGGSIGLSKSIKAIKLNLGSTALGNIEYRTFLNGSGWQ
                     TVVNSGRESNVPNESQQVEAIQMRLTGLLSKTYSVMYRVHMQDYGWQDWTYNNDIAGS
                     TNQGKRIEAIEIKLVETAKIPITVQTTYKGTGNYNVRVTNVISPGNLKIAIWSDKNNQ
                     DDLKWYTVTPKNHDATLTFNVTNHRDNGKYFIHVYQENASNQKQLLVKTSLQVAHSNY
                     NTPYFSQRDGRWASRRYGMATLGETGCVPTSLAMILSSLKGETVLPTQIADYLYHKTV
                     EFNRGVQGTTSRGILKAAKEWGVTATALGTQANLVQALKDGYHVLAAVQNNVFVLHGS
                     HEIVLKGYNNGLTHVSDPYTPSLSGWYPISQLWKEQSYYSEDRIDIGAPFVKVTDA"
     gene            complement(14050..16248)
                     /gene="recJ"
                     /locus_tag="VBC52_000570"
     CDS             complement(14050..16248)
                     /gene="recJ"
                     /locus_tag="VBC52_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEO6882174.1"
                     /translation="MISAKYSWVLNNQKPDAGFFEASKKEKISEAVASLIYSRGIKTS
                     AELHHFLQTNLENLHDPYLLNDMDKAVNRIRRAIENNETILVYGDYDADGMTSASIMK
                     EALDMMGAEVQVYLPNRFTDGYGPNESVYKYFIEQQDVSLIITVDNGVAGHEAIAYAQ
                     NQGVDVVVTDHHSMPADLPCAYAIIHPEHPDANYPFPYLAGCGVAFKVACALLETIPT
                     EMLDLVAIGTIADMVSLTDENRIMVKAGLEVMKDSERIGLQELISLSNIDLKTLNEET
                     IGFKIAPQLNALGRLDDPNPAIELLTGFDDEESQAIAQMIDQKNEERKEIVQTIFDQA
                     MQMLDQTKPVQVLAKENWHPGVLGIVAGRILERTGQPVIVLNIEDGIAKGSARSVEAL
                     DIFQAFDQHRELFIAFGGHSGAAGMTLEESKVGDLSQVLCDYISKKQLDMSQKKTLTI
                     DSELRFDELSLDTVRDFEKLAPFGMDNKKPVFLLKDFKVSQARVMGQNSAHLKLKLEQ
                     DGQALDLVAFNMGSQLQEFQQAQHLELAVTLSVNQWNGATTLQLMLEDARVDGIQLFD
                     IRSKASSLPHGVPILSQEEQSKEVILLTVPDHPQELKQMTQGKQFDAIYFKNEIPKNY
                     FISGYGTRDQFASLYKTIYQFPEFDVRYKLKELSSYLHIPDILLIKMIQIFEELHFVT
                     ITEGIMTVNKEAEKRDISESQIYQELKETVKFQELMALGTPKEIYDFMMS"
     gene            complement(16245..17006)
                     /locus_tag="VBC52_000571"
     CDS             complement(16245..17006)
                     /locus_tag="VBC52_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO6882175.1"
                     /translation="MARTILITGASGGLAQAIINQLPQDDHLIVTGRSREKLEKLYGK
                     RPNTLCLSLDITNDNAVTNTIEKIYGEFGQIDILINNAGFGSFKEFWDYSDEEVKDMF
                     AVNTFATMSIARQIGHKMSLVKSGHIVNIASMAGLIATSKASVYGASKFAVVGFSNAL
                     RLELAEKNVYVTSVNPGPIKTGFFAQADPSGDYLASIGRFALTPEKVAKKVVSILGKN
                     KRELNLPFILAFAHKCYSLFPKTADYFARKVFNYK"
     gene            complement(17008..17937)
                     /locus_tag="VBC52_000572"
     CDS             complement(17008..17937)
                     /locus_tag="VBC52_000572"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEO6882176.1"
                     /translation="MEIQFLGTGAGQPAKARNVSSLVLKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKVKKIFITHMHGDHVFGLPGFLSSRAFQANEEQTDLDIYGPVGIKSFV
                     MTGLRTSGSRLPYRIHFHEFDESSLGKIMETDKFTVYAEKLDHTIFCMGYRVVQKDLE
                     GTLDAEALKLAGVPFGPLFGKVKNGENVTLEDGREIIAKDYISEPKKGKVITILGDTR
                     KTDASIRLALGADVLVHESTYGKGDERIAKSHGHSTNMQAADIAKQANAKRLLLNHVS
                     ARFMGRDCWQMEEDAKTIFSNTHLVRDLEEVGI"
     gene            complement(17979..18662)
                     /locus_tag="VBC52_000573"
     CDS             complement(17979..18662)
                     /locus_tag="VBC52_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEO6882177.1"
                     /translation="MIDYIDLALTYGGFTSLDKVYLEKKLDGLSKQQRLDFITPPPSV
                     INAYFAEIYQKQGPEAATDYYFELSKALGLFPKHLSFDEEKPFIRLNLSGKSFGFAYL
                     NDQEEASVFSEVKEVITPQLLLEIAQIFPQYKVYQDRSGIRMAKIDFDETESQNITPE
                     TSLLGNVLQLKKDIIKITSFNQEELLELVKTKSGKYYYSSQGRESGKYYYSSQGRESV
                     IYIDTKSTN"
     gene            complement(18655..19893)
                     /gene="hflX"
                     /locus_tag="VBC52_000574"
     CDS             complement(18655..19893)
                     /gene="hflX"
                     /locus_tag="VBC52_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEO6882178.1"
                     /translation="MIETKEEQERVILVGVELQDTENFEMSMEELASLAKTAGANVVN
                     HYYQKRDKYDSKSFIGSGKLEEIKAIVEADEIDTVVVNNRLTPRQNSNLEAELGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQISDIERQLKIVEKNRETVRERRVDSTTFKIGLIGYTNAGKSTIMNVL
                     TDDKQYEANELFATLDATTKQIYLQNQFQVTLTDTVGFIQDLPTELVAAFKSTLEESR
                     HVDLLLHVIDASDPNHEEHEKVVMEILKDLDMIDIPRLAIYNKMDVTEQLNATTFPNV
                     RIAAKKQGSRDLLRRLIVDEIRHIFDEFSIRVHQNQAYKLYDLNKIALLDTYTFEEEY
                     ENITGYISPKQKWKLEEFYD"
     gene            complement(19984..20874)
                     /gene="miaA"
                     /locus_tag="VBC52_000575"
     CDS             complement(19984..20874)
                     /gene="miaA"
                     /locus_tag="VBC52_000575"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEO6882179.1"
                     /translation="MRKIKLIAVVGPTAVGKTALGIELAKTFNGEIISGDSQQVYQKL
                     DIGTAKASKEEQEQAYHHLIDVREVNENYSVYDFVKEAKVAIDTIISKGKIPIIVGGT
                     GLYLQSLFEGYHLGGEVNQETLMAYREKLESLSDEDLFEKLTEQSIVIPQVNRRRAIR
                     ALELAKFGSDLQNSESPYDVLLIGLNDDRQVLYDRINRRVDLMIDNGLLDEAKWLYDN
                     YPSVQASRGIGYKELFPYFSKQIPLEEAVDKLKQNTRRFAKRQLTWFRNRMNVEFIMV
                     GEENYQQKIKRKVSDFLSSK"
     gene            20985..21161
                     /locus_tag="VBC52_000576"
     CDS             20985..21161
                     /locus_tag="VBC52_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEO6882180.1"
                     /translation="MVKKFAFAKGIATGVVATAATLAGAAFAIKKTIIEPEEEKIAFI
                     EENRKKAARKRVSH"
     gene            complement(21203..22120)
                     /locus_tag="VBC52_000577"
     CDS             complement(21203..22120)
                     /locus_tag="VBC52_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEO6882181.1"
                     /translation="MKNWEKPPLVMAGLVLGLLALGNLLEGYGTYFRYCLGLVALVFW
                     IFLIKGILKNKKESRKELSNPLIASVFTTFFMAGMILSTYILLFRSLGIWVAVLSKGV
                     WWLSFIALIIHMAVFSWKYLRHFSMANLFPSWSVLYVGIGVASLTAPISGQFTIGKIV
                     FWYGFIATLVLLPFLFIKAYKIGLPSAVKPNITTICAPMSLITAGYVNSFVSPNRGLL
                     LLLIVMAQFLYFFILFQVPKLLIGDFTPGFSAFTFPLVISATSLKLSIQHLSLSVDIQ
                     GLVHFEIGTTTLIVMIVMVRYIFFLRRTI"
     gene            complement(22255..26017)
                     /locus_tag="VBC52_000578"
                     /pseudo
     CDS             complement(22255..26017)
                     /locus_tag="VBC52_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823026.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
     gene            complement(26186..26920)
                     /locus_tag="VBC52_000579"
     CDS             complement(26186..26920)
                     /locus_tag="VBC52_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882182.1"
                     /translation="MRDNHLHTHFSYDCQTAFEDYINGFTGEFITTEHFDLSNPYTGQ
                     DDVPDYSAYCQKIDYLNQKYGNRFKKGIEIGYFKDRESDILDYLKNKEFDLKLLSIHH
                     NGRYDYLQEEALKVPTKELLADYFNRMEFAIGRVEAHVLAHFDYGFRKLNLDVEDLKP
                     FETQLKRIFRKMLSKGLAFELNTKSLYLYGNEKLYRYALEILKQLGCKQYSIGSDGHI
                     PEHFCYEFDRLQGLLKDYQIDENHLI"
     gene            complement(26935..27519)
                     /locus_tag="VBC52_000580"
     CDS             complement(26935..27519)
                     /locus_tag="VBC52_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEO6882183.1"
                     /translation="MTDLEKIIKAIKSDSQNQNYTENGIDPLFAAPKTARINIVGQAP
                     GLKTQEARLYWKDKSGVRLRQWLGVDEETFYHSGKFAVLPLDFYYPGKGKSGDLPPRK
                     GFAEKWHPLILKEMPNVQLTLLVGQYAQKYYLGSSAHKNLTETVKAYKDYLPDYLPLV
                     HPSPRNQIWLKKNPWFEKDLIVDLQKIVADILKD"
     gene            complement(27616..29022)
                     /gene="pepV"
                     /locus_tag="VBC52_000581"
     CDS             complement(27616..29022)
                     /gene="pepV"
                     /locus_tag="VBC52_000581"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEO6882184.1"
                     /translation="MTIDFRAEVDKRKDALMDDLINLLRINSERDDSQADAEHPFGPG
                     PVKALEFFLEMAERDGYETKNVDNYAGHFTFGQGEEELGIFGHLDVVPAGSGWDTDPY
                     EPVIKDNRLYARGSSDDKGPTMACYYALKIIKELGLPTSKKVRFVVGTDEESGWGDMD
                     YYFEHVGLPKPDFGFSPDAEFPIINGEKGNITEYLHFSGENKGAVRLHSFSGGLRENM
                     VPESATARFTSHLDQTTLGASLADFASKHNLKAELSVEDGQYIATVYGKSAHGSTPQE
                     GVNGATYLALYLSQFDFEGPARAFLDVTANIIHEDFSGEKLGVAYEDDCMGSLSMNAG
                     VFQFDETNDDNTIALNFRYPQGTDAKTIQTKLEKLNGVEKVTLSDHEHTPHYVPMYDE
                     LVSTLIAVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPGDENTMHQANEYMPLE
                     NIFRSAAIYAEAIYELIK"
     gene            complement(29069..29671)
                     /locus_tag="VBC52_000582"
     CDS             complement(29069..29671)
                     /locus_tag="VBC52_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO6882185.1"
                     /translation="MKFLELNKKRHAVKHFNDKPVDFKDVRTAIEIATLAPSANNIQP
                     WKFVVVQEKKSALAEGLPESNCNQINQAQYVIALFTDTDLGQRSRKIARIGRRSLPDD
                     LIGYYMETLPPRYALYSEKQTGEYLSLNAGIVAMNLVLALTDQGISSNMILGFDKAIT
                     NDVLEIDKRFRPEILITVGYSDEKVEPSYRLPVDHIIEKR"
     gene            29841..31622
                     /locus_tag="VBC52_000583"
     CDS             29841..31622
                     /locus_tag="VBC52_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEO6882186.1"
                     /translation="MLRALKEDFFLTLTSLKNQFWVYIGTGILIQTLVAYLVKGILSF
                     IFRRILILSNTPAVTKDNWTLLFNHPLALLLFILYIVILIGFIYTEFAIYTIAILKTE
                     FSLSIKKWLTIFPKKIKSLLGPQLIWVSIYLLLTIPLANLGLRSSILEHLKIPDFISG
                     ELTKTVFGKIGYSTLLLLICYLNLRLIYFLPLTILTDYNAKEALLESWKLSRGKRQWR
                     LLSKIILTSLFISVIGTVALAVVAGTFSFIDHSGNNFPLQATFYNLLKSILFAVTLLI
                     KCLIISHLLADIKQNKSIVQQWRAWKGVHHKQKFKHIACVILALAIGTTALKNAIALA
                     LLQDNISIKKELIAHRGDTSHGVENSIEALKAAHKAKADYSEMDVIMTKDHKLVVIHD
                     DNLKRLSGMNKDVSKLTLDQVTKIPIHQGRFASYIPSFAEFMKTAQSLDQKIMIELKP
                     YNQNLDIYADEFIKEFKELRLSTKHKVMSLNLTLIEKVEKKLPQLDTGYLIPLHWGTL
                     QNHNVDFYGIEEFSYNDWIAYLAQEYNKQLYVWTINRDNLMIRYLQSPVNGIITDELN
                     LFKVINKDIKNSPNYYQRALQLIDSEG"
     gene            complement(31664..33445)
                     /gene="uvrC"
                     /locus_tag="VBC52_000584"
     CDS             complement(31664..33445)
                     /gene="uvrC"
                     /locus_tag="VBC52_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEO6882187.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKNGTIIYVGKAKNLKNRVKSY
                     FHGSHNTKTELLVSEIEDFEYIVTTSNTEALLLEINLIQENMPKYNIRLKDDKSYPYI
                     KITNERYPRLMITRQVKKSDGTYFGPYPDSGAATEIKRLLDRLFPFKKCTNPANKVCF
                     YYHLGQCNAHTVCQTNKAYWDSLREDVKQFLNGKDNKIVNGLTEKMKSAAMTMEFERA
                     AEYRDLIEAISLLRTKQRVIHQDMKDRDVFGYFVDKGWMCVQVFFVRNGKLIQRDVNM
                     FPYYNEPEEDFLTYIGQFYQDTKHFLPKEVFIPQDIDAKSVETIVGCKIVKPQRGEKK
                     QLVNLAIKNARVSLQQKFDLLEKDIRKTHGAIENLGNLLNIPKPVRIEAFDNSNIQGT
                     SPVAAMVVFVNGKPSKKDYRKFKIKTVIGPDDYASMREVIHRRYSRVLKDGLTPPDLI
                     VIDGGQGQVNIARDVIENQLGLAIPIAGLQKNDKHQTHELLFGDPLEVVELPRNSEEF
                     FLLHRIQDEVHRFAITFHRQLRSKNSFSSKLDGITGLGPKRKQLLMKHFKSLSSIQKA
                     DIEDIIMCGIPRNVAESLRDSLNDPPK"
     gene            33604..34371
                     /locus_tag="VBC52_000585"
     CDS             33604..34371
                     /locus_tag="VBC52_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEO6882188.1"
                     /translation="MIDVENILEKMKPNQKINYDWVMQQMVKQWQASDIRPKILMHVC
                     CAPCSTYTLEYLSQWADVTIYFANSNIHPKDEYYRREYVTQKFVHDFNKNTGYSVQFL
                     SAPYEPNEFFKIVHGLEEEPEGGDRCKVCYDFRLDKTAEKAVELGFDYFGSALTISPH
                     KNSQTINTIGIDVQKIYDTQYLPSDLKKNKGYQRSVEMCKDYDIYRQCYCGCIFGAKD
                     QGINLLQIKKDAKAFVSDKDGKEEFPNIRFTFNGKSM"
     gene            complement(34430..35770)
                     /locus_tag="VBC52_000586"
     CDS             complement(34430..35770)
                     /locus_tag="VBC52_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO6882189.1"
                     /translation="MYQTQTNKEKFVLFLKLFIPVLIYQFANFSATFIDSVMTGQYSQ
                     LHLAGVSTASNLWTPFFALLVGMISALVPVVGQHLGRGNKEQIRTEFHQFLYLGLILS
                     LILFLIMQFIAQPVLGSLGLEDEVLAVGRGYLNYMLIGIMPLVLFSICRSFFDALGLT
                     RLSMYLMLLILPFNSFFNYMLIYGKFGMPRLGGAGAGLGTSLTYWAIFIVIIIVMSLH
                     PQIKTYHIWTLERIKAPLIIEDIRLGLPIGLQIFAEVAIFAVVGLFMAKFSSIIIAAH
                     QAAMNFSSLMYAFPLSISTALAITISFEVGAERIQDANTYSRIGRLTAVGITSGTLLF
                     LFLFRENVAAMYNSDPHFVAITAQFLTYSLFFQFADAYAAPVQGILRGYKDTTKPFMI
                     GAGSYWLCALPLAVILEKNSQLGPFAYWIGLITGIFVCGLFLNQRLQKIKKLYY"
     gene            complement(35870..36286)
                     /locus_tag="VBC52_000587"
     CDS             complement(35870..36286)
                     /locus_tag="VBC52_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6882190.1"
                     /translation="MVMKAIEIYMNINGRGAEAITFYEEAFNTKAEHVTTFAQGWPDC
                     PPELEHLIMNAQFNIDGIRMQLSDNNPIFDFQIGQNMTAALIVESVEEAQELFNKLSR
                     DAMNIKMPLQAVPWSPAYGNVTDKYGMTWQINTELS"
     gene            complement(36290..36787)
                     /locus_tag="VBC52_000588"
     CDS             complement(36290..36787)
                     /locus_tag="VBC52_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6882191.1"
                     /translation="MIIDFKTVSTKGLETSPVAEALAGLRANEARYFWNKFKVAYETF
                     PVSDYPELMADINHLLKNERNLVFAAKPLEISQLFLENRILWTHVFYEDGLAINILYE
                     LDNPKKRAVGLKLSDGMDIPIELADKFKFARQKSKLAGIIRGSYFTLKGENETLMQRW
                     LDNMK"
     gene            37009..37605
                     /locus_tag="VBC52_000589"
     CDS             37009..37605
                     /locus_tag="VBC52_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEO6882192.1"
                     /translation="MTKKIFLFICATLLVGIALYTPTTSIFNHNNVYAATKKAKIKFN
                     KTQKKIVKKAREYAKSGHMSKDSIIEKLKKDSKKYRQEDINFVINNLKVDYKKNALIS
                     AKIYSKTMNLSKQSIFEQLYSESPDKATHSDKFTKEESQYAIDHLKVDFKENALETAK
                     SYQSSSSLSKEEIYKQLTSTLGDKFTNDEAQYAVDHLK"
     gene            37794..38416
                     /locus_tag="VBC52_000590"
                     /pseudo
     CDS             37794..38416
                     /locus_tag="VBC52_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612000.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        12329 a         7555 c         5933 g        12599 t
ORIGIN      
        1 ctcaaaacaa gagagtaaaa agttaatttt gaacgatttt atgcacaaca tgacctggcc
       61 attctatcaa atcctgtgct aaaaaattag gattacaatc aatttttgtt aattctttta
      121 ttggaagcca tacaaaatgt cgcttatgcg ttccttcttt gatagattct ggcgcctgac
      181 ccaagagatt aacaaaatat aaaaattcaa ctgagtggta atgatgtctc ttaatattaa
      241 actgattctc aacaatatag gctaaatcac caacctggct gtgaagacct atttcctcaa
      301 gtacttctct cgcaacagcc ttattgctat cttctccaac ttgaagcgcc ccacctggta
      361 aatagtattt atcgagatga ggagcatata ttaatagcag tttttgattt cctatgatta
      421 aagcagaaac tctgactccg aatgtttcga gctgttctct gaccctaaaa tctctatttt
      481 tcataaaaat taaccgtctt ttcaattaaa ttttggtgtt catcataaac aaacttagca
      541 gaaaagaaag gaacatcttc taaaatccat ttaaaaattt cgtaatctcc ttgccacgtt
      601 ggcttagaga gaacttgatc atatggtacc cattctaaag ttccttctcg tgattcatca
      661 tctgaaatta actctccttc ataatctgtt actttaaaga cataggtata ccaatcatga
      721 cctggcgtaa attcaggaaa agtgatgact cctttaaaat ccattttttt tactgttaaa
      781 tgggtttctt ctagaatttc acgcttagca cattcatcag gagtttcacc tgcctccagt
      841 ttaccgccta ccgagatcca ctttccctca tgaacatcat tctctttttt gttgcggtgc
      901 aatagaagta attccttgcc attgtcaatg taacaaattg ttgctaattt agtcatttca
      961 aactcctctc attctcatat tataacaaaa aatagccttt atttaggcaa tttactatca
     1021 aagtagcaca caaatgatta aacaaattat tttgtttacc agttatttac ttttcacttt
     1081 gatgacattt taaaggttct ttatcataaa cctttaaagc atgctataat taggaaaaac
     1141 cggtttacaa ttttaaaacc taaggagtaa aaaatggaaa ttaaaaagaa acatcgtatt
     1201 atgctttatt cagcccttat tttaggaaca atattggtta acaatagtta ccaagctaaa
     1261 gctgaagagc ttaccaaaac tacctcaacg tcccaaataa gagatactca aactaataat
     1321 attgaagttc cccagactga aagtaccact gtcaaagaga ctagcaccac aaccacacaa
     1381 caagatctgt ctaaccccac agcttcaacc acaactgcaa cagccactcc tagcacaatg
     1441 aaacaagtag tagataatca aactcaaaat aaggagctgg tgaaaaacgg agattttaat
     1501 caaactaacc ctgtatctgg aagctggtca catacaagcg ctagggaatg gtctgcttgg
     1561 attgataaag gaaacactgc tgataaatca cctattatcc aacgtaccga acaaggccaa
     1621 gtaagtctat ccagcgacaa aggctttaga ggtgctgtaa cacaaaaagt gaacattgat
     1681 cccactaaaa aatatgaggt caagtttgat attgaaacaa gtaacaaggc tggacaagct
     1741 ttccttcgta ttatggagaa aaaagacaac aatacgcgac tttggctttc tgagatgacc
     1801 agcggtacta ctaacaaaca taccttaaca aagatatata acccaaagtt agatgtctcc
     1861 gaggtgacac ttgaacttta ttatgaaaaa ggaacaggtt ctgttacttt tgataatata
     1921 tcaatgaaag caaaaggccc taaagacgca gagcatccac aacccgtcac aacacaaatt
     1981 gaagaaagcg ttaatacggc tttaaacaaa aattacgttt ttaataaagc tgattaccag
     2041 tacactctaa ccaatccgtc tcttgggaaa attgttggtg gaatattgta tccaaacgct
     2101 actggttcaa caactgttaa aatatctgat aaatctggca aaataattaa agaagtaccg
     2161 ttatcagtta cagcttcaac agaagataag tttacaaaac tcctcgacaa atggaacgac
     2221 gtgactattg gtaatcatgt ttacgatact aatgattcga acatgcaaaa gcttaatcag
     2281 aaattagatg aaactaacgc caaaaatatc gaagctatca aactggattc taatcgcact
     2341 ttcctttggg aagacttgaa agatctcaat aattcagcac agttaactgc tacttatcgt
     2401 cgtttggaag atttagctaa acaaatcacc aatccccact ctactattta caaaaatgaa
     2461 aaagctattc gtactgtaaa agagagtctg gcttggcttc atcaaaactt ctacaatgtt
     2521 aataaagata tagaaggctc tgccaattgg tgggattttg aaatcggtgt ccctcgctca
     2581 attacaggta ccctagctct catgaataac tacttcactg acgctgaaat aaaaacttat
     2641 accgacccaa ttgaacactt tgttcctgat gcaggatatt tccgtaaaac gcttgtcaat
     2701 ccatttaaag cccttggtgg taatctagtc gatatggggc gcgttaaaat cattgaaggt
     2761 ttacttcgta aagacaatac tattatcgaa aaaacttctc attctctaaa aaatcttttt
     2821 actactgcta ctaaagctga aggcttctat gctgacggtt cttacatcga ccatacaaat
     2881 gttgcttata ctggcgccta tggtaatgtt ctgatagatg gtttgacaca attgctgcct
     2941 atcattcaag aaactgacta taaaatctct aatcaagaac ttgatatggt ttataaatgg
     3001 attaatcaat catttttacc tttaattgta aaaggtgagt taatggatat gagtcgtgga
     3061 cgctcaatta gtagagaggc agcttcttcg catgcggctg cagttgaagt tctcagaggt
     3121 ttcctcagat tggctaacat atctaatgaa gagcgaaact tagacctcaa atcaactatt
     3181 aaaacgatta tcacttcaaa taaattctac aatgtcttca ataacctcaa atcgtattcc
     3241 gatattgcca acatgaataa gctgcttaat gacagtacag tcgctactaa acctttaaaa
     3301 agtaatttat caacctttaa tagcatggac cgcttagctt attataatgc cgagaaagac
     3361 tttggtttcg cgctttcatt acattctaaa cgtaccctca actatgaagg aatgaatgat
     3421 gaaaatacac gtggttggta taccggagat ggtatgttct atctttataa tagtgatcaa
     3481 tctcattata gtaatcattt ttggccaacc gtcaatcctt ataaaatggc tggaacaact
     3541 gaaaaagatg ctaagcgtga agataccact aaggaattca tgagcaaaca tagcaaagac
     3601 gctaaagaaa aaaccggtca agttacagga acatctgact ttgttggttc cgtcaaactt
     3661 aatgatcact ttgctcttgc cgctatggat tttactaact gggatcgcac cttaacagca
     3721 caaaaaggtt gggttatctt aaatgataag attgtctttt taggtagcaa catcaagaat
     3781 actaacggca ttggaaatgt ttctacaaca attgatcaac gaaaagacga ttctaaaaca
     3841 ccttatacta catacgtcaa tggaaaaact attgatttaa aacaagcaag ttctcaacaa
     3901 tttacagata caaaaagtgt ctttttagaa tcaaaagaac ctggtcgcaa tattggttat
     3961 atcttcttta aaaatagcac tattgatatt gaacgcaaag agcaaacagg tacttggaac
     4021 agcattaatc gtacttctaa aaatacctca atcgttagca atccttttat cactataagc
     4081 caaaagcatg acaacaaaga tgatagctat ggttacatga tggttccaaa cattgatcgc
     4141 acaagttttg ataaattagc caacagcaaa gaagtagaat tactagaaaa tagttcaaaa
     4201 caacaggtta tctatgataa aaacagtcaa acttggactg ttatcaaaca cgataatcaa
     4261 gagagtctca ttaacaatca attcaaaatg aataaagcgg gactttacct agtacaaaaa
     4321 gttggtaatg actatcaaaa tgtctattac caacctcaaa ccatgacaaa aacagaccaa
     4381 ttagctatct aactagccgt acataaaaaa cctatcatca aaatgatagg ttttttggtt
     4441 caattactta atgacttctt gagttttagc atacttggct tctacagcat ctttttctgc
     4501 tttccaccat gtctcatttt ctgtatacca tttaatagtt tcttccaaac cttctgaaaa
     4561 gtttgtaaat tgtggttccc agccgagttc ttcacgcaat tttgtagaat cgatagcgta
     4621 acgaagatca tgaccagcac ggtcagttac gtgatcgtaa gcgtcttttg gttgacccat
     4681 tttctcaagg ataagttcaa gaacttcctt gttgtttttc tcaccgtcgg caccaataag
     4741 gtatgtttca ccaatacgac ccttagtcaa aatagcccag acacctgttg aatggtcatt
     4801 tgtgtggatc cagtcacgga cgtttttacc ttcaccataa agtttcggtt tgattcctgc
     4861 caagatattt gtaatttgac gtggaatgaa tttttcgatg tgttggtatg gtccatagtt
     4921 atttgaacag ttcgaaatgg tcgcttttac gccgaaggaa cgtacccatg ccttaacgat
     4981 aaggtcagaa gctgccttag ttgatgagta aggtgatgat ggattgtatt ttgtttcagc
     5041 agtgaatttt tcacctggtc cttcaccatt tcctggaagg tcttcacgaa gtggaagatc
     5101 tccataaact tcatcagttg acacgtggtg gaaacggata tcgtatttac gagctgcttc
     5161 aagcaaagtg taagttccga taaagtttgt atggataaat ggacttggat cttccaatga
     5221 gttatcgttg tgactctcag ccgcatagtg aacaatagca tccgctttgg cagccaattt
     5281 gtctaccaat tcagcgtcag caatatcacc aacaactaac tcaacacgat caccaagaat
     5341 ggcttcaatg ttagcacggt tacctgcata tgtcaattta tcaaggacag ttacatgaac
     5401 atctgggtgg ttattgtaaa catagtgtac aaagtttgat ccgatgaaac cagctccacc
     5461 agttacgata atgtttttaa attcagtcat tttttctccg aaaaattttt tctttttgct
     5521 gtttgaagga tactgttctg ttagaagtag ttctatggaa tatcatgcct aagaaatcaa
     5581 tatttcaaat ctctgagaga acaggccttt tgcatgtttc ctttcaccct tgcgtcaaac
     5641 aacattgcta attcgttttt acttgctaag attaatgcct tttaagaagt tgaaaattat
     5701 aaatcttcct tcttcaaagg ttttacatct ttgagaagcg ggtgattctt atctgcttct
     5761 gagacttctg cttcttcaag gttttcccac tgaatgccta gatttgaatc tgcatagtta
     5821 acgaaagcat attttggttt gagttcaagt gcccaataat cgttaacgag ataagtataa
     5881 gctgctttat ctgaaagaac ttggaaacca ttagcaacgc cgcgtggaac aaagatacct
     5941 tttgaggcat cgataatcgt ttggtaaacg ttcccaaaac tgtcaccttc acggaggtca
     6001 acccaagtac cgatcacacg tccttcatca gcgatcgaaa catatttatc ccaaggctct
     6061 gcatggagac ctcgtaaagt attttttttg ttaaacgaaa tattattttg tagtttgtct
     6121 gcctcaaaga agctttctgg gaaaccaagc ggtatcattt tttctttttg aaagttctct
     6181 ttgaaccatc cacggttgtc tccacgaacg ggaatatcga attccaacaa tccaggaatg
     6241 gcttcaattg ggcgacaagt taattcttta tcaaaaaatt gttcagtcat taggcttctc
     6301 caatcaaacg aagtaggtat tgaccatact catttttctt aagtggttgt gccaactcaa
     6361 ggacttgttc acgagtgatg tagcccatgc ggtaagcaat ttcctcaagg ttagcaactt
     6421 gaacgttttg catgcgttgt actgtttcaa tatattgtgc tgcttcaaga agactttcgt
     6481 gagttccagt gtctaaccac gcaaatccac gtcccattaa ctcaactgat aagtcacctc
     6541 tatcaaggta ggctttgtta acatcagtaa tttcaagttc tccacgagga cttggcttaa
     6601 tatttttagc tatttcaacc acatcgttat catagaaata aagacctgtg acagcataat
     6661 tagacttagg ttgcgctggt ttttcttcaa tagaaatggc attcatatca gtatcaaact
     6721 cgacaacacc aaaacgttct ggatctttta cttgatagcc aaagacggtt gctccacttt
     6781 ctttactggc tgcacgttgt agcattgcag ataaacctgg tccatgataa atattatcac
     6841 ctaatactaa ggcaacgtga tcatcgccaa taaaatcttc accgataatg aaggcctgtg
     6901 ctaacccatc tgggcttggt tgttcagcat aagaaagaga gattcctaat tctgaaccat
     6961 ctccaagcat atcttcaaag cgaggcaaat cttgtggagt agaaataatc agtatttctt
     7021 ttattcctgc caacatcaaa actgacaatg gataataaat cattggttta tcataaattg
     7081 gcatcaattg ttttgatgca gcacgagtta atgggtacaa gcgagtacct gaacctcccg
     7141 ctaaaataat acctttcata gtaaggtctc cttatttata ttaatcctac atattataac
     7201 acttttttta aacgctatca tctactaatc tttttcacgt atttcgtaat atcgtaatcc
     7261 tctaaattta gctgattttc tttatttaag attaattgac ataattcata accaattaat
     7321 gggcctactg ttaagccagt tgatcctaat ccacttgcag catatgcacc ctccatacaa
     7381 gggacagggc caaaaaatgg agaaaaatca cttgtatagg cacgaattcc aacacgttca
     7441 gaagtcgtat ctgctttttt taattgcgga aaatagccaa tagcttgctc ttcaaacttg
     7501 tctaagactt taaaatcaat gcttaaatca aaagccatat cattttcgtg acttgcacct
     7561 atagagactt taccattatc aaatgggata atatctagct caccttctgg cattacaaca
     7621 ggatattttc ctgtattaat attgctaaaa taatagtctc gcaattgccc tttttgaggg
     7681 cgaacatcta cctgatagtt gtgctcatct aatatcttag ctagccaggc tccactcgct
     7741 aaaacaagtt tatcaaaatc aattccttga acacgaaagc cattatcagt tatcgtaaag
     7801 tgaacttcat ccttgatgac attaacacca cttgcttcta acaatattcg tgttaagtcc
     7861 gccccttcta ctcgtgctcc tccgctcgca taaagtaact gttcatagcc atctaactca
     7921 ggaaagtgag tatttgcttc acttttattt agtatttgta aatcgcctat tagaggagat
     7981 tctagtcgac gtttatctgc tagtgcaaat aatgactcta actgactctc gtcctttttt
     8041 aataagaaaa caccagtttg ctgataaaat tttgtttcaa aaccatcttt ttgtaaatcc
     8101 gtgactagct ttgaataaaa atcagccccc aacctcgcca tacgatacca ggctttatta
     8161 cgacgttttg aaaaccatgg gctaataatc cccgcagcag ccttagttgc ttgtccaact
     8221 ccataatcaa aaacagtaac ctgaatatcc ttttcttttg ataagtaata ggctagtgtc
     8281 gctccgactg ctcctgcacc aataattgct atctttttca taatagctcc tacatatggt
     8341 aaaagggatt tgttggagct ttactctcta aaactgaaat atcccagtct ttttcaagtt
     8401 tccactgctc aatcatagta gctattttag aaacaaataa aacctcaata tgatgacctg
     8461 ggtcaatcgc aagcaaacca tttgtgatca tttcttgtgc agtatgataa taaatatctc
     8521 ccgttacaaa aacatctgct ccttttgcaa tagcttcttt gtagaaactt tgcccactac
     8581 ccccacaaat agcaactcgt tgaatttcag gattagattt atcatatgaa actaaacgaa
     8641 cgctatctaa accaaacaca tctttaattt tccatgcaaa aaattcaaaa ctctggggtc
     8701 taacatcacc aatgcgacca ataccataac cattacttgt ttcagataat atatcgggat
     8761 attgaatatc taataattca caaaaccagt cgttaagtcc attaggaacg atatctatat
     8821 tagtatggct cacataaaca gcaatgtctg atttaagaag gtctatataa atcttatttt
     8881 gcggtgttgc taccaaatcc tttaatggac gaaaaatagg agcatgcttt acaatcagaa
     8941 gatctacttg acgttcaatt gcttcagcta ctgtggtctc tcgaacatct aaagctacca
     9001 tgactgtctt tatttcttta tctaaagatc caatttgaag acctgaaata tctccttcca
     9061 tagataattc ctgtggacaa taagtttcat atacatcaat aagttctcta gctttcatca
     9121 agaacctcct ttatatcctg tattttatct tccaaaatag cgcgttcttc aattttacta
     9181 ttaggaatag agtttaaagc aaaagttaat ttattaagct cgttttgcca tttttcttta
     9241 aaaaccgttg tgttattgga taataaaaat ggaccaaatc gcaattcttt agcagttaaa
     9301 ttcatatgac catgcttaac aactaaaatt tcatagcatt tgtcattttc agtcaagata
     9361 tcttctgcta ctatttcaaa atcatttgca gctaaccatt ttcttaaatc atcttcccga
     9421 ttattaggtt gtaagactag tgttttaacg tactgaagtt tgtctatatc gttattcaaa
     9481 atatcagcaa tcaaacgacc acccattcca cagatagtta tcgtatcaat attatctgct
     9541 tcttcaaaag ctgacaaacc atttgctaag cgaacatcta tctttgaagt gagaccatgt
     9601 tctgaaacgt ttttcaacgc agactgatac ggtccattca ccacttcacc agcaatggca
     9661 aaatcacagt atcccatctg caacaaaaaa ataggtaagt aagcatgatc acttcctaca
     9721 tctaataacc gagctccttt tggaacatag tttgcaactt tttgtaatcg ttttgataat
     9781 tgtaaatcca ttgtatttcc ttcagttttt ctctattaaa agtataacat cattaacatc
     9841 aaaagaaaag gagcagacct aatgtatcaa ccattctgtc tcctcctagt aaacttattc
     9901 ccataaattc attgcatttt taaaatcatc cgaaatcgct aaatctttca tttggttatc
     9961 ttcacgtgct tgttttctct cttcaatttg tcgaagtgtc gtcaatcctt cacgccgcca
    10021 gttgcgaagg atagcattga tataattcca acttgttttc ccattgaaaa ccgcttctct
    10081 aagagctgcc ctaacaatat caggatctgt ctgatcttct tgcagtgtct tttgtaaatc
    10141 ttctaattca aaaggactta ataagcgacc taattcacgt tcgaagtctc ctactaaatc
    10201 tttcagacga ttcgaatcag aggttttatt gtctattatt acttgattat cctcttcgaa
    10261 aagtttatct aatttagcaa acacagggct tgtatcaaag ataatctcga tttcatgatt
    10321 caactcaatt gttttgacat ctagtaaacc ttgacttgtt aaattcgata tagaacgatt
    10381 gatatctgcc actgttttat caagtgatgt cgcaatacgg ctcggtgtta gatcggataa
    10441 attagttgta ttttgtaaat agaaaaattg ccaaactaaa aaatcgtctg ctgtcttaaa
    10501 aatagattta aagtgaaaaa ataaagcact tggtagagtc aattgccctg actgatattg
    10561 ttccaaataa gtcataacct ttcaaatcct ctctatcgtc ctaaaattat gaaatgtgta
    10621 acaaaaagca cctctcaact attatcatag cacttagagg cgcccttatc attcatctat
    10681 cttgaatagg ccttatcctg gaaagttcat caatacctta gtatcataac cttcaatagc
    10741 tttacgacca tttaatccat caagttcaac taggaaagca cagcctgcga caacgccacc
    10801 gagtttttca atcatttcaa ttgtcgcttt tactgttcca ccagtagcta aaaggtcatc
    10861 aacaattaaa acacgttgac ctggtttaat agcatctgcg tgcattgtca atgtatcaag
    10921 accatattct ttttcatagt cagctgaaat aacttcgcgt ggtaatttac ctggtttacg
    10981 gactggagca aaaccaatac caagagcata agcaactggg caaccaacaa taaagccacg
    11041 agcttctggt ccaacaatca tatcaatatc tttatcagca gcatattgta caatttcacg
    11101 aacagcatag ctgtatgctt taccatcagc cattaatggg gagatatcac ggaaagtaat
    11161 tccttcttga ggatagtttt caattgaagc gatgtaatta tttaaatcca tttcacttgt
    11221 ctttctaagg ggaaacccct aaaatgtttt actcttcatt atatcataaa attaaataaa
    11281 atagtactgg aaactttaaa accatatgct agttttgcat atggttttaa aaattaggca
    11341 tctgttacct tgacaaaagg tgctccaata tcaattctat cctcactata atagctctgt
    11401 tctttccaaa gttgtgaaat tggataccaa ccagacaagg acggtgtgta agggtcagag
    11461 acatgtgtca gtccgttatt atatcctttg agaacgattt catgacttcc atgaagaaca
    11521 aaaacattat tttgtactgc cgctaggaca tgataaccat ccttcaacgc ttgtacaagg
    11581 ttagcttgag ttcctaatgc agttgcagtt acaccccatt ctttggctgc ttttaaaatt
    11641 cctctagaag ttgttccttg aacaccacga ttaaattcaa ctgtcttatg gtaaaggtaa
    11701 tctgcaattt gcgttggtaa aacagtttct cctttcaaag aagacaaaat catagctaga
    11761 gaagttggta cacaaccagt ttctcctaaa gttgccatac catatcttcg acttgcccac
    11821 cttccatcac gttggctaaa gtaaggagta ttataatttg aatgtgcgac ttgtagagaa
    11881 gtttttacca ataattgttt ttgattagaa gcattttcct gataaacatg gataaagtat
    11941 ttaccattgt ctctatgatt tgtaacatta aatgtcaatg tagcgtcatg atttttgggt
    12001 gtgacggtat accacttaag gtcatcctga ttgtttttat ctgaccaaat agcaattttt
    12061 aaattcccag gactaataac atttgtcact cgtacattat agtttccagt tcccttatag
    12121 gtcgtttgga cagttatagg tattttagca gtctcaacta atttaatttc gattgcttca
    12181 attcgttttc cttggttagt actaccagcg atgtcattgt tataagtcca atcctgccaa
    12241 ccataatctt gcatgtgaac tcgatacatc acactatagg ttttcgatag cagacctgtc
    12301 aatcgcatct gaatagcttc tacttgttga gattcatttg gtacattact ttctctccct
    12361 gaattaacaa cagtttgcca acctgaacca ttaagaaaag ttctatattc tatatttcct
    12421 agagctgtac tacctaagtt aagtttgatg gcttttatag attttgaaag tccaatacta
    12481 cctcctaatt ggccttccaa aaccgttggt ttccaaccat ctttttgaag ataagtttga
    12541 taacttaatc caggatgctt catttctaag aatgaatgat ctgtttggga actaaaatct
    12601 gaagctttag ctactagttt tacttccaaa gcctctaaac gtttcgattc tccgactgta
    12661 cctgctacac tattattttt ttgccaagcc atccaacctt tatcttggac gtgagcgcga
    12721 taataaatat cataattttg ttttaaattc ccacttaagt ccaattgaat agcttctagt
    12781 ctttttcctt gataatcctt acttgtttga ctacctaaaa tttctcccca acctttatct
    12841 tgtacatgag tacgatagag aatgtcccca tacttcaagg tgctaagagt gaattttact
    12901 ccatctaggg ctttggattc tcctaaagtt cccgccattt gtccttcaag tttattagat
    12961 tgccaaccat tttggcctac cttaacctga taatttaaga caggtctctc ttccttaagt
    13021 atagttggtg ttaaaacaga ttgtccttta agggtaaact ttacttcgta agcttcaagg
    13081 cgctttgaca tttccaaagt cccagcgtaa gctccatttt tagcccaagc catccacccg
    13141 atatcttgaa catgaacacg ataatagaca tcatataatt tttgtaaatt atcagtaagg
    13201 ttaatgctta gtgcttcaat aggtttactc tgaccaacag taccactaac ttgaccactc
    13261 tctactgaag attgccaacc ttgtccttta acataagttt tatagctaag ttttcctgtt
    13321 attccttcag gtgctttaat agacaatttt attgcctcaa cctgcttttc ctgtcctact
    13381 gttcctgaaa ctgttgcatt atcaaccgac ttttgccatc caatatcttg aacatgagca
    13441 gagtaagtca ctgttggtaa ttgctttgtt gaattttgtt gagtaataac ttcactatca
    13501 ttagctactt gattaacatt agaatttgtt tgatttgctg agtgctcagc agtactagct
    13561 tttacaacaa cttttgagct agcatcaaag gttttgactg gggtagctgc tatctctcta
    13621 ggattagatg aaacagatgt agcaaagcct gtttttatta aaggctctgc actatttttc
    13681 atagcctccg aggaattaac aggtaacgtt aacttggaag tatccgaaat agtctgactg
    13741 ctatcaattt tctctgagat cgttgaagtt actgcttctg cctgttctgc cttgacatct
    13801 tgaccaataa ttgcactacc acctaaaata gtaacgatac ctgcaatgac ccaaaattta
    13861 ccttttttgt acatcttttt tctaacgata ggtctcatag attttatcct cacctttaat
    13921 attctttatc aaaaattcca ttattttctt aattttatta tattttaata atatttgcaa
    13981 tattattatt gaaataactt atttatatac caaaaaaacc acttaaaaag tggcttcttt
    14041 agtatattat caactcatca taaaatcata tatttcttta ggtgtcccta aagccattaa
    14101 ttcttgaaac ttaactgtct cttttaattc ttgataaatt tgactctcag agatatctcg
    14161 cttttctgct tctttattga ctgtcataat cccttctgta atcgtaacaa aatgtagttc
    14221 ttcaaaaatt tgaatcattt taattaataa aatatcaggg atatgaaggt aggaactgag
    14281 ctcttttaac ttataacgaa cgtcaaattc tgggaattga tatattgttt tatataaact
    14341 agcaaactga tcacgtgtac cataaccaga tataaaataa ttttttggta tctcattttt
    14401 aaaatagatg gcatcaaact gtttaccttg agtcatttgt tttaattctt gtggatgatc
    14461 gggaacagtc aaaagaataa cttctttgct ctgctcttct tgagatagga ttggcactcc
    14521 atgaggtaag ctacttgctt tgcttctaat atcaaataat tgaataccgt ctacacgcgc
    14581 atcctccaac attaattgta gggttgtggc accattccat tgattaacag ataaagtgac
    14641 agctaactcc aaatgttgtg cttgttggaa ctcttgtagt tgactcccca tattaaatgc
    14701 taccaaatca agtgcttgtc catcttgttc tagttttaat ttcagatgag cactgttttg
    14761 ccccatgact ctagcttgac tcactttaaa atcttttaac aaaaagactg gctttttatt
    14821 atccattcca aatggcgcta acttttcaaa atctctaaca gtgtctaaac taagttcgtc
    14881 aaaacgtagc tcactatcaa ttgtcagagt ttttttctga ctcatgtcca attgtttttt
    14941 agagatatag tcacataaaa cttgagaaag atcacccacc ttactttcct ctaaagtcat
    15001 gcctgctgct ccgctgtgac ctccaaaagc aatgaataat tctcgatgct ggtcaaaagc
    15061 ttgaaaaata tcaagtgctt ctacacttct ggcacttcct ttagcaatac catcttcaat
    15121 attcaaaaca ataacaggtt gtccagttct ctctaaaata cgacccgcca caatacctaa
    15181 aacccctgga tgccaatttt cttttgctaa aacttgtaca ggctttgttt gatcaagcat
    15241 ttgcatagct tggtcaaaga tagtttgaac gatttctttg cgctcttcat ttttttgatc
    15301 aatcatttgg gctattgctt gagattcttc atcatcaaac cctgtcagta attcaatagc
    15361 tggattaggg tcatcgagtc ttcctaaagc gtttaattgt ggagcaattt taaatccaat
    15421 agtttcttca tttagtgttt ttaaatcaat atttgataaa gatattagct cctgtaagcc
    15481 tattctttca ctatctttca taacttcaag tccagctttt accatgatac gattttcatc
    15541 agttaggcta accatatctg caatcgtccc aatagcaacc aaatccaaca tttctgtcgg
    15601 gattgtttct aagagagcac aagctacttt aaaagcaaca ccacaccctg ctaaataagg
    15661 aaatggataa ttagcatcag gatgttctgg gtgaataata gcataagcac aaggtaaatc
    15721 tgctggcata ctgtgatgat cagtaactac cacgtccaca ccttgatttt gagcatatgc
    15781 aattgcttca tgccctgcca caccattatc aaccgtaatg attaatgaaa catcttgttg
    15841 ttcaataaaa tatttataaa cactttcatt gggaccgtaa ccatctgtaa aacgatttgg
    15901 gagataaacc tggacttcag ctcccatcat atctaaagct tctttcatga ttgatgctga
    15961 tgtcattcca tcagcatcat agtctccata aactaaaata gtttcattat tttcaatagc
    16021 tcgtctaata cgattaacag ctttatccat atcatttaac aaataaggat catggagatt
    16081 ttccaaatta gtttgtaaaa aatgatgtaa ttccgctgat gtcttaatac ctcgcgaata
    16141 aattaaggaa gccaccgctt cacttatctt ttcttttttt gaagcctcaa aaaagccagc
    16201 gtctggcttt tggttattga ggacccaact atacttagca gatatcattt ataattgaat
    16261 acctttctgg caaaataatc agctgttttt ggaaataaac tataacattt atgcgcaaaa
    16321 gctaatataa aaggaagatt taattctctc ttattttttc caagaataga aactactttt
    16381 ttagctactt tttctggtgt tagagcaaaa cgacctatac ttgctaaata atcaccactt
    16441 ggatcagcct gagcaaaaaa tcctgttttg attggtccag ggtttacact tgtcacataa
    16501 acattttttt ctgctaattc taaacgaaga gcattcgaaa atccaacgac agcaaactta
    16561 ctagccccat aaacactagc tttacttgtt gctatgaggc cagccatgct tgcaatatta
    16621 acgatatgac cagattttac aagagacatc ttatgtccta tctgacgagc tatagacatc
    16681 gttgcaaatg tattcacagc aaacatgtct ttgacttctt catcactata atcccaaaat
    16741 tctttaaaag aaccaaatcc agcattatta attaaaatat caatttgacc aaattcacca
    16801 taaatttttt caatcgtgtt cgttacagca ttgtcattag ttatgtctag tgataaacat
    16861 aaggtatttg gtctctttcc ataaagcttt tcaagttttt ctcttgatct ccctgtgaca
    16921 attaaatggt cgtcttgagg taattggtta ataattgcct gtgctaaacc accagaagca
    16981 cctgtaatca gtatcgttct tgccatttta tatacctact tcttccaaat ctctaacaag
    17041 atgggtattt gaaaaaatcg tctttgcatc ttcttccatt tgccaacaat ctcgccccat
    17101 aaaacgagca ctaacatgat ttaataataa tcgtttagca tttgcttgtt tagcgatatc
    17161 agctgcttgc atattggttg aatgcccatg cgatttagca attcgctcat cacccttacc
    17221 ataagtggat tcgtgaacta aaacatcagc gcccaaagct aacctaatac ttgcatctgt
    17281 ttttctagta tctcccaaaa tagtgattac tttacccttt ttcggttcag aaatataatc
    17341 tttagcaata atttctctac catcttctaa ggtaacattc tcaccatttt tgactttccc
    17401 aaaaagagga ccaaatggaa cacctgctag tttcaatgct tcagcatcta aagtgccctc
    17461 aaggtctttt tgtacaacac gatatcccat acaaaagata gtatgatcta gcttttctgc
    17521 ataaacggta aatttatctg tttccataat ttttccgagt gatgattcat caaactcgtg
    17581 aaaatggatt cgataaggta aacgtgaacc tgatgtcctt aagccagtca taacaaaact
    17641 ttttatacca accggtccat aaatatctaa atctgtctgt tcttcattgg cttgaaaagc
    17701 ccgactggat aaaaaacctg gaagaccaaa aacatggtct ccgtgcatgt gcgtgataaa
    17761 aatttttttg actttacgag gtttaatcgt ggtttctaaa atttgacgtt gtgtaccctc
    17821 tccacaatca aacatccaaa cctcgttaat ttcatctaat aatttcaaga ctaaacttga
    17881 aacattacga gctttcgctg gttgaccagc tccagttcct aaaaattgaa tttccatact
    17941 tgttgacttt tttataatcc ttccataact taataaattt aattagtaga tttagtatca
    18001 atataaataa ctgactcacg tccttgacta gaataatagt atttacctga ctcacgtcct
    18061 tgactagaat aatagtattt acctgacttt gttttaacca actctaatag ctcttcttga
    18121 ttgaaactag ttattttaat aatatctttt ttaagttgta agacattccc tagtaaactt
    18181 gtttctggtg taatattttg agattctgtt tcgtcaaaat ctatttttgc cattctgata
    18241 ccagaccgat cttgatagac tttatattga ggaaaaatct gtgcaatttc taagagtaac
    18301 tgcggtgtaa tgacttcttt aacttcagaa aaaacactcg cttcttcttg atcatttagg
    18361 taagcaaaac caaatgattt tcctgataaa ttcaaacgaa taaaaggttt ttcttcatca
    18421 aacgataaat gttttggaaa caagcccaag gctttggaaa gttcaaaata gtaatcagtt
    18481 gccgcttctg gcccttgctt ttgatatatt tcagcaaaat aagcattaat cacactagga
    18541 ggtggggtga taaaatcaag tcgttgttgc ttagagaggc catctagttt tttctctaga
    18601 taaactttat ctaagctcgt aaaaccacca taagtcaaag ctaaatcaat ataatcaatc
    18661 ataaaattct tccagcttcc atttttgttt aggagaaata tagcctgtaa tattctcgta
    18721 ttcttcttca aaggtgtagg tgtcaagcaa tgctatttta ttgagatcat agagcttata
    18781 tgcttgattt tgatgcactc taatcgaaaa ttcatcaaag atatgtcgta tttcatccac
    18841 aataagacgg cgtaataaat ccctactccc ttgttttttg gctgcaattc tcacattagg
    18901 aaaagttgtt gcattcagct gctcagtgac atccatcttg ttgtatatcg ctaaacgagg
    18961 aatatctatc atatctaagt ctttaagaat ctccataaca actttttcgt gttcttcatg
    19021 attagggtca ctagcatcaa taacatgaag taataaatcg acatgtcgac tttcttctaa
    19081 ggtagacttg aaagccgcaa ctaactctgt aggtaaatct tgaataaaac caacagtatc
    19141 tgttaaagtg acttgaaatt gattttgtaa atagatctgc ttggttgtag cgtctaacgt
    19201 tgcaaataat tcattggctt catattgctt atcatcggtc aaaacattca taatagttga
    19261 cttgccagca tttgtatatc caattaatcc aattttgaag gttgtcgaat caacacgtct
    19321 ttctcgaact gtctcgcgat ttttttctac aatcttcaat tgtctttcga tatcagaaat
    19381 ttgatgacga atggaacgac gattcaactc taactgagat tctcctggac ctcggcttcc
    19441 gattccccct gcttgacgac taagcataat accttgtcca actaagcgtg gtaacatata
    19501 tttcaattgc gctagatgaa cctgtaattt tccttcatga cttctagcac gcatagcaaa
    19561 gatatctaaa ataagttgca tacggtcaat aactttaaca ccaagttcag cttccaaatt
    19621 agagttttgt ctcggagtca aacgattatt aaccacaacg gtatcaatct catctgcttc
    19681 tacaatagct tttatctcct ctaatttacc agatccgata aatgatttac tatcatattt
    19741 atctcttttt tgataataat gattaacaac atttgctcct gctgtcttag ctaagctagc
    19801 aagttcttcc atggacattt caaaattctc agtatcttga agttctaccc caactaaaat
    19861 aacacgttct tgctcttctt tagtttctat cacctataat ctcctttcaa tatttttttc
    19921 ttgttctaaa tacgtaatct ttattataac ataatgccct ttaaatgcga gaaacggctt
    19981 tatttacttt gatgataaaa aatcactgac ttttcgcttg attttttgtt gataattttc
    20041 ctcaccaacc ataataaatt cgacattcat acggtttcta aaccacgtta attggcgttt
    20101 tgcaaaacgt ctggtatttt gttttagttt atcaacagct tcttctaatg gtatttgctt
    20161 tgaaaagtaa ggaaataatt ctttatagcc aattcctcta cttgcttgaa cactagggta
    20221 gttatcgtat agccatttag cttcatctaa gagaccattg tctatcatca aatcaactcg
    20281 tctattgata cgatcataaa gcacctgacg atcatcattc aatccaatta ataaaacatc
    20341 atacggactt tcactatttt gcaaatcact cccaaacttc gccaattcta atgcacggat
    20401 ggcacgtcga cgattaactt gcggtataac aatggattgt tctgtcaact tttcaaataa
    20461 atcttcatca cttagagact ctaatttttc acgataggcc ataagagttt cttgattgac
    20521 ttcgcctcct aaatgatagc cctcaaacaa actctgtaaa tataggccag tacctccaac
    20581 aataataggg attttcccct tagaaataat cgtatcaata gctacttttg cctctttgac
    20641 aaaatcataa accgaatagt tctcatttac ttcacgaaca tcaatcaagt gatgataagc
    20701 ttgttcttgc tcttctttac tagctttagc cgttccgata tctaacttct gataaacttg
    20761 ctgactatca ccactaatga tttcgccatt gaaagtttta gctaattcaa ttcctaatgc
    20821 tgtcttccca accgcagttg gaccaacaac tgctataagc ttgatttttc tcatgatact
    20881 tctcaattct ataaaggttt ttacttgaaa tttaactagt atttcgttac cataattata
    20941 tcatagatag aagcagagtc ttcatcatag attggagaat tattatggtt aaaaaattcg
    21001 catttgctaa aggaatagca acaggagtcg ttgcaacagc tgctactctt gcaggtgctg
    21061 catttgctat caaaaaaaca attattgaac ctgaggaaga aaaaatagct tttatcgaag
    21121 aaaatcgtaa aaaagctgca cgtaaacgtg tctctcatta aacaataact cccttactag
    21181 ctagtaaggg agttatttta tattaaattg ttcgccttaa aaagaaaata taacgtacca
    21241 ttactatcat aacaataaga gttgttgtcc caatttcaaa gtggactagc ccctgtatat
    21301 caactgataa tgatagatgc tgaatactta atttaagtga ggtagctgag ataacaaggg
    21361 ggaaagtaaa ggctgagaaa cctggtgtaa aatcaccaat cagtaattta ggcacctgaa
    21421 aaagaatgaa aaaataaaga aattgtgcca ttacgattaa tagcaataat aaacctctat
    21481 ttggtgacac aaaactattg acatatcccg ctgtgattag cgacattgga gcgcaaatag
    21541 tagttatatt aggcttaact gccgaaggta gcccgatttt atatgcttta atgaatagaa
    21601 atggtaataa tactaaagtt gcaataaaac cataccaaaa gactattttc ccaatagtga
    21661 attgtccact aattggtgct gtcaggctag ctacccctat accaacatag aggacagacc
    21721 aagaaggaaa aagattagcc atcgaaaagt gtctcaaata tttccatgaa aaaacagcca
    21781 tatgaatgat taacgcaata aatgacaacc accatacacc ttttgacaga actgctaccc
    21841 atattcctaa acttctaaat aataagatat aggttgataa aatcatacct gccataaaaa
    21901 aagtagtaaa gacagaagca atcaaaggat tgctcaactc cttcctgctt tcttttttat
    21961 tttttaatat tcctttgatt aaaaaaatcc aaaaaacaag cgctactagc cctaaacaat
    22021 agcgaaaata ggtgccatat ccctctagaa ggttccccaa agctaatagc cccaatacta
    22081 atcccgccat cactaatggt ggtttttccc aatttttcat catatacctc ataaaaaaac
    22141 gtttacatct attataacac aattagattt tgctgataag tgattttatt aacttaatga
    22201 aaagaagcta gcccattgac taacttcttt tttaagctct tctaaagttt gcttttattt
    22261 taagtgacga ttcctcttta aagagagacc tagtagagca agtagacttg ctagaatgct
    22321 tattcctact acacttagta tttttgatga cttatctcca gtttttggca aattagcttc
    22381 atttgtcaga tatgaatcgg ccttcttaga aactacagga attattgagg tcttattagc
    22441 tattctattt actttttcta ccgttttaga ttccttgaaa gattgggttt tggggtaatc
    22501 tgcttgcaat tcctccttag gactaggggc aactttgcca actttaatca caattgctgt
    22561 taatggatca atcagcaagc tatctttttc aaaatgaaca cctctaggag ttgagattgg
    22621 tttaatcccc gcctgatcag catcaacaat tacttgccct tttaaaaggt gtttatattt
    22681 ttcacctagt aaaacgtttc tagctttact atcagcatta acaaatactg cgtaaatatc
    22741 acctttagaa tctattgttt ggtaagcaat aaccaaatct ttttctttga tatcaccttg
    22801 acctacctct gtaatcaagc taacctcaag atcaattttt gctttgctca atttccggaa
    22861 agcatctgtt gaacgcctta atgtgattaa acctgctgta taggcctgtg ttttcgttga
    22921 aattgggtgt ttgttattat ctgtggctgc tgcccaatca aaatgattaa tggcatctga
    22981 agaatcatat gaatcatgaa taaaatatgg gtattcttta acagcttcaa taagtgttgc
    23041 tttattaggc aatttgtcat ctgaaacttt tgtcatgtaa tcagggttaa gtaaacgctt
    23101 cgtacgacca tactcttgac cagaatgaat gaatgctgtc ccttgagatg ttaaaatcat
    23161 tacatttcct aaacgcagac gtctatgaat ctcttcttca gctaccttag ggtctttatt
    23221 aattgatttt gcaatcacat catgcaaggt aaggttatca tgtgcagcaa tatactgcac
    23281 cacatctcct ggcgaatctg cttcaaaatt cccaggttgt gctttgatat ttttaaaaat
    23341 accttgtaaa gattgtgggc cacctgtgat gaaagctgga gtaccttcat ttggaaaacc
    23401 agatttcaag ctattacgaa tgtcatctga aaagacgcca actgtatcgg ttgacttcat
    23461 ccaatcttgg tcagctggtt taaccggctt accttgatca ccttggaatg ttctccagcc
    23521 ctcaccaatc ataatcatat taggattaat agctttagct tctttataag ctaattcaat
    23581 cgcagccgca tcatgatctc ccatcatatc aaaacggaaa ccatcaactt taaattcatt
    23641 tgtaagatat ttaatggaat caaccaaaac acgacgactc attgcatgag tggttcctaa
    23701 acgtccccct ccaaaacttt ctcttggtga accatcttca ttcataaagt gataataatt
    23761 aggttctata tcctcaaaga gataagtttt tgcagtgtga ttatagacga catcaagtat
    23821 aacacccatg ccacgtttat gaatatcatg tattaattgt tttaattcgg caatacgtgc
    23881 tgatggatct tttggtttct ctgaatacat tccagaaaga gcaaaatagc tctgtgggtc
    23941 atagccccaa ttgtaattat tgtctgagga agtgtaagct gttgagcgtg acttatccat
    24001 ttcattaaca taaaaataac tcaataccgg taaaagctga atgtgtgtaa ctcctaattt
    24061 ctgtaaataa tctagtttct ctgaaaaggc tgcaaaggta ccaaattgat tttttaattt
    24121 tccgtccaaa gatcgatcag aagtgaagtc tcttacatgt gcttcgtata taacagcatc
    24181 ttgtcttcct ttaaaattag caattttagc aaaacttaaa ttttgaggtc caagttgact
    24241 tggatttaca aaagctgctt tagccgtttt tatatcgtca ttaacagtat tactatccca
    24301 ctctgctaat gactttgcat aaggatctaa aatcttaacc ttatccttac ctctttttat
    24361 ttcgtaaaga taatagtaac cagtatagtt tttaatacct aatttagtat caagtatcgt
    24421 ctgccaaaca cctttattat ttttcacaag gggtgtagtc gctacaaccc tgttttgatt
    24481 atctttgtca taaataatca tagtgacact atcagcactc ggtgaccaga ggctggcttc
    24541 aacttttgaa ccatcttgat tgaaaactgc acctaaattt ccactataag cataaagttg
    24601 gtctttaaat tcccaagatt gccttgtcat gacattgtta ccattataag atatgttgaa
    24661 atgaccttgt ttaggattaa agtcgccttt gattattaaa agagatttac tagtatcgag
    24721 agtgatatca gaaatttgta tagcattttg atttttatca gtcactttca attcttttaa
    24781 aatttcagtt ttatctaccc catctagagt tgtaaaactt gcttgaatac ttgttaaatc
    24841 aatttgttgg gcatccttta gctgcacttg atcaatgtaa taaggattat tataaacctt
    24901 tggatcctta tcttttacaa aaatttggtt atggttagct aaatctctaa aaacatagtc
    24961 gttgggttga actttcactg catctcctgt cttactttca tctaagatta gaaaaccaat
    25021 ctctttggcg ttagttttta gtggtacatc aatatacctt ccatatagtc cttgattcac
    25081 aaaattacta ccatctggcc aagttgttga ggggtttgca acatctttaa agagccatgc
    25141 tgataggtgg tcatagttac cagaggaact caaatagtta atacggacat acccttcttt
    25201 gaggggctga taagtatgta taccgtactt ttcatcaatc caaacttggt tcatctcagg
    25261 tcgtaataat gaaatatgat gatcgccgct taaatttgtt cctgctttgt tattaattaa
    25321 aaaagatatt tgttttcgtt gtttttcaga taatttaaaa tcaacataat aaccgtaatc
    25381 atctttctta gcatcagtca tagggatagc accatttggc caattactag acggttgatc
    25441 aacatctccc catatccaaa gtccagtgct ttctacagga tgaccttgag gcaattcttt
    25501 aagatgtagc ctaaagtaac cattttcaat ctgtggagct tgattagcaa ctttcaaggt
    25561 cggacttgga gcacctatag cttgagaagt tttgtcggga gtattttgca atgtagatgg
    25621 tgccactttt aaatctaagg tatttttatc tgaaacagga cctttatttt cttcaagcag
    25681 gcttgtcgcc tgccttatcc ggctctgtca ctcccttttg agtgtcagtt tcatctgata
    25741 ctaattgctt ggtatccggc atatctgagg gtgtcacaac cgtctcagag tttgagttta
    25801 tgtcatcttt taaattagta gattgttggt tgtcaacctg attaatattt gactctgcaa
    25861 tcgctgtagt tgcaggagaa acacttactt cttcagcaaa tacctgtgga ctatgaagaa
    25921 aaatacatat ccctgttgca actgaagcta ctccaacact taactttcta atcgtgtatt
    25981 gagtttgttt atcaccaaat aagtcttttc ttttcatatc attctccttt tttaatgaat
    26041 tgttaccaat aaaatcataa caatttacgc aagcgcttgc aataagtaaa tgatttcttt
    26101 atttttttga aattttcact aaaactatca atgacattac aaaaaggcta agcaatattg
    26161 cctagctttt ttatcaaaaa tttcctcata tcaaatgatt ttcatcaatt tgatagtcct
    26221 ttagcagacc ttgaagtcta tcaaattcat aacaaaaatg ttcaggaata tgaccatcag
    26281 agcctataga gtattgttta caaccaagct gttttagtat ctctaaagca tagcgataaa
    26341 gtttttcatt cccatataga taaagggatt tggtatttag ttcaaaagct aaccccttag
    26401 ataacatctt tctgaaaatg cgcttcaatt gtgtttcaaa cggctttaaa tcttctacat
    26461 ctaagtttaa cttacgaaaa ccataatcaa agtgagctaa aacgtgcgct tccacacggc
    26521 ctatggcaaa ttccatacga ttaaagtaat ctgctaaaag ctcctttgtt ggtactttca
    26581 gagcttcttc ttgcagataa tcatacctac cattatgatg gattgacaat agttttaaat
    26641 caaattcttt attttttaaa taatctaaaa tatctgattc cctatcttta aaataaccga
    26701 tttcaattcc ttttttaaat cgatttccat atttctgatt aagataatct attttttgac
    26761 aataagcact ataatcagga acatcgtctt gaccggtgta aggatttgat aaatcaaaat
    26821 gttctgttgt gataaattca cctgtaaaac cattaatata gtcctcaaat gccgtttgac
    26881 aatcatagga aaaatgcgtg tgtagatgat tatctctcat accaactcct atccttaatc
    26941 ttttaaaata tctgctacta tcttttgtaa atcaacgatt agatcttttt caaaccatgg
    27001 attcttcttt agccaaattt gatttcgcgg tgatgggtga accaggggta aataatcagg
    27061 tagatagtct ttgtaagctt taactgtttc tgttagattt ttatgtgcgg agcttccaag
    27121 ataatatttc tgagcatatt gaccaactag caaggtcaat tgaacattag gcatttcttt
    27181 taaaataaga gggtgccatt tctccgcaaa accttttcta gggggtaaat ctcctgattt
    27241 tcctttgcct gggtaataaa aatctaaagg caaaacagca aattttccag aatggtaaaa
    27301 tgtctcttca tcaactccaa gccactggcg tagacgaact ccagatttat ctttccaata
    27361 gagtcttgct tcttgagttt ttaaaccagg tgcttggcca acaatattga tcctagctgt
    27421 tttaggagca gcaaacaaag gatcaatacc attttctgta taattttgat tttgtgaatc
    27481 actttttatt gctttaataa ttttttccaa gtcagtcata aagcctccaa atctaacctt
    27541 atgaaaagag gcaaagacac tttcatgtct tcaccaccct ttatcattga tcacatattt
    27601 agtttaagga ttattttatt tgattaattc atagatagct tctgcgtaga tagcagccga
    27661 acggaaaata ttttctaaag gcatgtactc attagcttga tgcatagtgt tttcatctcc
    27721 tgggaacatg gcaccgtatg caacaccccg ttcaagtaag cgaccaaatg tcccaccacc
    27781 aataacctgt tcatgtcctt taagaccagt ttgcttttca tagacagcta ttaaggttga
    27841 tactaattca tcgtacatag gtacatagtg tggtgtgtgt tcatggtcag aaagagtcac
    27901 tttttcaact ccgttaagtt tctcaagctt agtttggata gttttagcat ctgtcccttg
    27961 tgggtaacgg aaattaagag cgatagtatt atcatcatta gtttcatcaa actggaagac
    28021 acctgcattc atgctcaacg atcccataca gtcatcttca taagctactc caagtttttc
    28081 acctgagaag tcttcgtgaa taatgttggc tgtgacatct aagaaagcac gagcaggacc
    28141 ttcaaaatca aattgactta gataaagagc taaataagtc gccccattaa caccttcttg
    28201 tggtgttgaa ccatgagcag atttaccata aactgtagct atatactgcc catcttctac
    28261 agataattct gctttcaaat tatgtttact tgcaaaatca gctagactag ctccaagtgt
    28321 tgtctgatct aaatgacttg tgaaacgtgc tgtggctgat tctggaacca tgttttcgcg
    28381 aagtccgcca gaaaagctat gcagtctaac agctccctta ttttctcctg aaaaatgtaa
    28441 atattcagtg atattacctt tttcaccatt tataattgga aattctgcat ctggagagaa
    28501 accaaaatca ggttttggaa gaccaacatg ttcgaagtag taatccatat ctccccatcc
    28561 agattcttca tcagttccta caacaaaacg aactttctta gatgttggta gcccaagctc
    28621 tttaataatt tttagagcat aataacatgc cattgttggt cccttatcgt ctgatgagcc
    28681 tcgagcatat aaacggttat ccttgataac tggttcataa gggtctgtat cccaaccgct
    28741 gcctgcagga acaacatcta aatgcccaaa aatacctagt tcttcttcgc cttgaccaaa
    28801 tgtaaagtgt cccgcataat tatctacgtt ttttgtttcg tagccatctc gctcagccat
    28861 ctcaaggaaa aattccaagg cttttacagg tccaggacca aatggatgct ctgcatcagc
    28921 ttgtgaatca tcacgctccg agttaatgcg aagaagattt attaaatcgt ccatcaaagc
    28981 gtctttgcgt ttgtctactt ctgctctaaa atcaattgtc attacggcaa ctccttctat
    29041 aaaatattat gtagtttgtg cttaaaaatt aacgtttttc aataatatga tcaaccggca
    29101 aacgataact aggctcaacc ttttcatcac tataaccaac agtaattaat atttctggtc
    29161 taaaacgttt atctatctct aagacatcat tggtaatcgc tttatcgaac cctaaaatca
    29221 tattagaact aataccttga tctgtcaagg caagtactaa attcatagca acgatacctg
    29281 catttaaaga gagatactct ccagtttgtt tttcactata taaagcatag cgtggtggta
    29341 gagtttccat gtaataacca attaaatcat caggaagaga tctacgacca atacgagcta
    29401 ttttccgtga tctctgtcct aaatctgtat ctgtaaataa agcaataaca tactgcgctt
    29461 gattaatttg gttacaatta ctttccggta aaccttctgc tagagcagat tttttttctt
    29521 ggacaacgac aaacttccaa ggctgaatat tattagcaga tggcgctaat gtcgcaattt
    29581 caatagcagt tctaacatct ttaaagtcga ctggtttatc attaaaatgt ttcacagcat
    29641 ggcgcttctt atttaattct agaaatttca taaaaacctc ctattctatt tgattttccc
    29701 tactattcta tcacttttta aaatgcgttt cagcttttga aggcaaaatt gataaaaaag
    29761 tgaaaccgtt ttgaaaacta ccataaaaaa caaacaaact tgtggtacaa tatctaatga
    29821 ctataaaaaa ggagctattc atgctacgag cattaaaaga agattttttt ctaaccttaa
    29881 cctctttaaa aaatcaattt tgggtttaca ttggaactgg tatcttaatc caaactttag
    29941 ttgcttattt agtaaaagga attctgagtt ttatctttcg tcggatattg atattgtcaa
    30001 atactcccgc agtgactaaa gacaattgga ctttgctttt taatcatccc ttagctttat
    30061 tactctttat actttatatc gtaatcctaa ttgggttcat ctatactgag ttcgctatct
    30121 atactattgc catactgaaa actgaatttt ctctcagtat caaaaaatgg ctaacaatct
    30181 ttcctaaaaa aataaaatca cttttaggac cacagctaat ttgggtatca atctacttac
    30241 ttttgacaat tcctctagct aaccttggtc taaggtcaag tatactagag catcttaaaa
    30301 tccctgactt tatttctggc gagttaacaa agacagtctt tggaaagata ggttactcta
    30361 cccttttact gcttatttgt tacttaaacc ttcgattaat ttattttctc ccgctgacca
    30421 ttttaactga ctacaatgct aaagaagcac ttttagagag ttggaaactt agccgcggaa
    30481 agcgtcagtg gcgtcttcta agtaaaatta ttttaaccag tttatttata tctgtaattg
    30541 gaacagttgc actagctgtt gttgctggta cattttcatt cattgatcat tctggaaaca
    30601 attttcctct tcaagccact ttctataacc ttttaaaatc aatacttttt gccgtaacac
    30661 ttcttattaa atgtttaatc ataagtcacc tattagcaga tattaaacaa aataaaagta
    30721 ttgttcaaca atggagagct tggaaaggcg tccaccataa gcaaaaattt aaacacattg
    30781 cttgtgttat tctagctcta gctattggta ccactgcctt aaaaaatgct atagctctgg
    30841 ctctcttaca agataacatt tcaattaaaa aagaattaat agcccatcgt ggtgatacat
    30901 cacatggcgt tgaaaattct attgaagcat taaaagcagc ccataaagca aaagcagatt
    30961 attcagagat ggatgttatc atgacaaaag atcataaatt agtagtcatt catgatgata
    31021 atctcaaacg actctctggt atgaacaaag atgtttctaa actaacttta gatcaagtta
    31081 caaaaatccc aattcaccaa gggcgttttg ctagttacat tccatcattt gcagaattta
    31141 tgaagaccgc tcagtcacta gatcaaaaaa tcatgattga attgaaaccg tataatcaaa
    31201 acctcgatat ctatgcggat gaatttatca aagaatttaa agagcttcgt ctatcaacca
    31261 agcataaagt aatgtctctc aacttgacac tcattgaaaa ggttgagaaa aaattgccac
    31321 aacttgatac aggttaccta atcccactac attggggaac gcttcaaaat cataacgttg
    31381 acttttatgg gattgaggaa ttttcataca atgattggat tgcatatctc gcccaagaat
    31441 ataataaaca gctctatgtt tggactatta accgtgataa ccttatgata cgttatttac
    31501 aaagcccagt taatggaatc atcacagatg aactcaatct tttcaaggtc attaataaag
    31561 atatcaaaaa tagccctaat tattatcaaa gagctctcca acttattgat agcgagggtt
    31621 aaattaaata aaaagcttag tagaacagta tctactaagt tttttattta ggaggatcat
    31681 taagagaatc tcggagactt tctgctacat tcctaggaat accacacata ataatgtcct
    31741 caatatcagc tttttggata ctagataagc ttttaaaatg tttcattagc aattgtttac
    31801 gtttgggtcc caacccagtg ataccatcta attttgaaga aaaggaattt ttggagcgta
    31861 attgacgatg aaaagtaata gcaaaacgat gtacctcatc ttgaatacgg tgaagtaaga
    31921 aaaattcttc tgaattacgc ggtagttcta ctacttctaa aggatctcca aaaagtaatt
    31981 catgtgtttg gtgtttatca ttcttttgca aacctgcaat aggtatggct aaacctaatt
    32041 ggttctcaat aacatcacgc gctatattta cttgcccctg accaccatca attacaatca
    32101 agtccggagg tgtcaagcca tcttttaaaa ccctactata acgccgatga atgacttctc
    32161 tcatactagc ataatcatct ggtccgataa cagtcttaat tttaaacttt cgatagtctt
    32221 ttttacttgg ctttccattc acaaatacca ccatagcagc tactggactt gtcccttgaa
    32281 tattggaatt atcaaatgct tctatgcgta ctggtttagg gatattaaga agattaccta
    32341 aattttcaat tgccccatga gttttacgaa tatctttttc cagtaagtca aacttctgtt
    32401 gtaaactgac acgagcattt tttattgcca gatttactaa ctgtttcttt tcaccacgtt
    32461 gtggtttaac aatcttacag cctacaattg tttcaaccga cttagcatca atatcctgtg
    32521 gaataaagac ttctttgggt agaaaatgtt ttgtatcttg ataaaattgc cctatgtaag
    32581 ttagaaaatc ttcctctggt tcattataat aaggaaacat attcacgtca cgctgaatca
    32641 attttccatt tcgaacaaaa aagacctgta cacacatcca acccttatca acgaaataac
    32701 caaaaacatc tctatccttc atatcttgat gaataacacg ctgcttagta cgcaacaaac
    32761 taattgcttc gatcaaatct cgatattcag ccgcacgctc aaattccata gtcatagcag
    32821 cgctcttcat tttttcagtt aatccgttaa caattttatt atctttacca ttaagaaact
    32881 gttttacatc ttctctcaga ctatcccaat aagctttatt agtttgacaa actgtatgtg
    32941 cattgcattg ccctaaatga taataaaagc atactttatt tgctggatta gtacatttct
    33001 taaaaggaaa gagcctatct agtaaccgct taatctcagt tgctgcccca gaatccggat
    33061 aaggtccaaa ataagtgcca tctgattttt ttacttgacg tgtaatcata agacgaggat
    33121 aacgttcatt tgttattttg atatatgggt aagacttatc atctttcaat cgaatattat
    33181 acttcggcat attttcttgg attaaattaa tttccaataa tagagcttcg gtattagatg
    33241 ttgtcacaat gtattcaaag tcttcaatct cagagaccaa aagttctgtc ttagtattat
    33301 gactcccatg aaaataactt ttcacacgat tcttaagatt tttagcctta cctacataaa
    33361 taattgtccc attcttatct ttatgaaggt aacaaccagg actgtctggt aacaattcta
    33421 gcttatgttt aatcagttca ttcatagaac tattataaca aaatgtactg taatctttac
    33481 gaaataaata ctattcccca acatctattg tatcttccaa tacttgaata aaaccccaat
    33541 actcctagtc taagcaatta ttttatgtta cactttaaac aagtgaatta ttgaggagtt
    33601 tttatgatag acgttgagaa tattcttgaa aaaatgaaac ctaaccaaaa aatcaattac
    33661 gattgggtca tgcaacaaat ggttaagcag tggcaagcta gtgatatccg tccgaaaatt
    33721 ctgatgcatg tttgctgtgc tccttgtagt acatataccc ttgaatactt atcacaatgg
    33781 gcagatgtta ccatctattt tgctaattca aatatccatc ctaaagatga atattatcgc
    33841 cgcgaatatg ttactcaaaa atttgttcat gatttcaaca aaaatactgg atattctgta
    33901 caattcttat cagcaccata tgaaccaaat gaatttttca aaattgttca tgggttagaa
    33961 gaagaacctg aaggtgggga tcgttgtaaa gtttgctatg atttccgtct tgataagaca
    34021 gcggaaaaag ccgttgaact aggttttgat tactttggca gtgccctaac tattagtcct
    34081 cacaaaaact ctcaaacaat caatactatt ggtattgatg tccaaaaaat ttatgatact
    34141 cagtatcttc ctagcgacct taaaaaaaat aaaggttacc aacgttccgt tgagatgtgt
    34201 aaagattatg atatctaccg acaatgttac tgtgggtgta tcttcggtgc taaagatcaa
    34261 gggataaatc ttctccaaat caaaaaagat gcgaaagctt tcgtgagtga taaagacggc
    34321 aaagaagaat ttcctaatat acgcttcaca tttaatggca agtcaatgta aatgataaaa
    34381 aaagacttag taaagaacta agcctttttg gtataaatct tgtcaaactt taataataca
    34441 acttcttaat cttttgcaga cgttggttta gaaatagacc acaaacaaaa atacctgtga
    34501 ttaaaccaat ccaataggca aacggaccta actggctatt tttttctaag ataaccgcta
    34561 atggcaaagc acataaccaa taagagcccg caccgatcat aaatggtttt gttgtatcct
    34621 tatagcctcg taaaatcccc tgtacaggag ctgcataagc atctgcaaac tggaaaaaga
    34681 gactataagt taggaattga gctgtaatag cgacaaagtg agggtcacta ttatacattg
    34741 ctgctacatt ctcacgaaat agaaataaaa atagtaaggt tcctgatgta atccctaccg
    34801 ctgttaagcg tcctatccta ctataagtgt ttgcgtcctg aatgcgctct gcccctactt
    34861 caaacgatat tgtaatagct agagcagtgg aaatacttaa aggaaaggca tacattaatg
    34921 atgaaaaatt catagcagcc tgatgtgctg caataatgat tgaagaaaat tttgccatga
    34981 ataagcctac tactgcaaaa attgcaactt ctgcaaaaat ttgtaaacca atcggtaatc
    35041 ccaatcgaat atcttcaata atcaaaggag cttttattct ttccagagtc catatatgat
    35101 atgttttaat ttgaggatga agtgacatca caataataat aacaataaaa atagcccaat
    35161 aagttaaaga agttccaaga cctgcccccg cacctcctag tctaggcata ccaaatttac
    35221 cgtagataag catataatta aaaaatgaat taaagggtag aattaaaagc atcagataca
    35281 tagataacct tgttaacccc aatgcatcaa agaatgaacg gcaaatgcta aacaacacca
    35341 gcggcatgat tccaatcaac atataattta aataaccacg accaactgct agaacttcat
    35401 cttctaaacc caaactcccc aagacaggtt gagcaataaa ttgcatgatt aaaaataata
    35461 ttaaggacag tatcaaacct aaatatagaa attgatgaaa ttctgtgcga atttgttctt
    35521 tatttcctct acccaaatgt tgaccaacta ctggtactaa tgctgaaatc atacctacta
    35581 ataaagcgaa aaacggagtc cataaattac tagcagttga tacacctgcc aaatgtagct
    35641 gactatactg tccagtcata accgaatcaa taaaagtagc tgaaaaatta gcaaattgat
    35701 aaatcaatac tgggataaat aatttcaaaa ataaaacaaa tttttcctta tttgtctgag
    35761 tttgatacat cttttttctc cttttcacct taatcaattt attttagctt agctaggata
    35821 ctctgtcaat gctatttaaa aaggttgaga aataatcctc aaccccattt tagctaagct
    35881 cagtattgat ttgccatgtc ataccatatt tgtcagtcac atttccataa gctggactcc
    35941 acggaactgc ttgtagaggc attttaatgt tcattgcatc tcgagaaagc ttattgaata
    36001 actcttgcgc ttcttctaca gattccacaa ttaaagctgc tgtcatattt tgtccaattt
    36061 gaaaatcaaa aataggatta ttgtcagaaa gttgcattcg aataccatca atattaaatt
    36121 gagcgttcat aattaaatgt tcaagttctg gcggacaatc tggccaaccc tgtgcaaaag
    36181 ttgtgacgtg ttctgctttt gtattaaaag cctcttcata aaatgtaata gcttcggccc
    36241 cacgaccatt aatattcata taaatttcaa ttgctttcat taccatacct cacttcatat
    36301 tatccaacca acgctgcatc agagtttcat tttccccttt tagggtaaaa taagaaccac
    36361 gtattatacc tgcgagtttt gacttttgac gagcaaactt aaacttgtcc gctaattcaa
    36421 taggaatatc cattccatct gataatttta aacctacagc tctcttttta ggattatcta
    36481 attcatataa aatattaatg gctaaaccat cctcatagaa aacatgtgtc cataaaatac
    36541 gattctctaa aaaaagctgt gaaatctcta aaggctttgc agcaaaaaca aggttacgtt
    36601 cattttttag aaggtgatta atatctgcca tcaattctgg atagtctgat actggaaatg
    36661 tttcgtaggc tactttgaat ttattccaga aatagcgagc ttcatttgca cgcaaaccag
    36721 ccaaggcttc tgccactgga gaagtctcca aacctttagt tgaaacggtt ttaaagtcga
    36781 taatcatgtt ctttcctttt tgattaaaat cttcgataat ttagtatact cttttttgaa
    36841 gaaaaagtac attctaatca cttataaaaa gtagtacatt tagaacacta ccacctcagt
    36901 aatttcttga cctttctcta cttaatactt ataattaatc tagacttaat gtcaaagcat
    36961 caatcaaggt gtaaacctga ttcacctaaa aagctgatgg agctaaacat gaccaaaaag
    37021 atattcttat ttatctgcgc tactctttta gtaggaatag ccttatatac accaacaact
    37081 tctattttta atcataacaa cgtttatgct gccaccaaga aagcaaaaat taaatttaat
    37141 aaaactcaaa aaaaaattgt caaaaaagca agagaatatg ctaagtctgg ccatatgtct
    37201 aaagatagta tcattgaaaa attaaaaaag gattctaaga aatatcgtca agaagacatc
    37261 aactttgtga ttaataacct caaggttgat tacaaaaaaa atgccttgat ttctgccaaa
    37321 atttattcaa agaccatgaa tttatctaaa caatctatct ttgaacagct ctattcggag
    37381 tctccagata aggcaaccca ctctgataaa tttacaaaag aagaatccca atatgctata
    37441 gaccatctca aagtagattt caaagaaaat gctcttgaga ctgctaaaag ttatcaaagt
    37501 agttcttctt tatccaaaga agaaatctat aaacaattga catctacgct gggcgataaa
    37561 ttcacaaatg atgaagccca atatgctgta gatcatttga aatgatgaca taccaatagt
    37621 ctatccttat gtaatagaat tgatagctta tgctaatatt ccagctgtaa aatagcaaga
    37681 accctcctaa gttcattaag acttaggagg gtttttatca atatgaatag gttacactaa
    37741 actagacaga aattataaag tgttctacac ttaagaaaaa ctggagaaaa accatgtcta
    37801 gaaaagtacg tcgtcacttc accgatgatt ttaagcaaca aattgttgac ctttacaatg
    37861 ttggtagaaa gcgtagcagt ctcatcaagg aatatgagct aaccccttcc accttcgata
    37921 agtgggttag acaagccaaa acaactggtt cattcaagtc tgttgataat ctgacagatg
    37981 aacagcggga gctaattgaa ttcagaaaac acaataaaga actcgaaatg caattagata
    38041 tcctaaagca agcagcggtg attatggcac aaaaagggaa ataatcactg ctaataagga
    38101 taaatacagc atttcaaaga tgtgtcgctg gctgaatatg ccacgctcaa gttattacta
    38161 ttcaagccgt ggagtcagta tctgaaacgg agtttgaaga aactattaaa agaatttttc
    38221 tcgatagcga gtctagatac ggatccagaa aaatcaaaat atgcttgaat aacgaaggta
    38281 tcacactttc acgtcgtcgg attcgacgca ttatgaagcg actcaatttg gtttctgttt
    38341 atcagaaagc caccttcaaa ccacattcta gaggcaagaa tgaagcccct attcccaacc
    38401 acttagacag gcaatt
//
LOCUS       DATRNK010000011        37873 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.011, whole genome shotgun sequence.
ACCESSION   DATRNK010000011 DATRNK010000000
VERSION     DATRNK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37873
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.011"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..2791)
                     /gene="ppc"
                     /locus_tag="VBC52_000591"
     CDS             complement(<1..2791)
                     /gene="ppc"
                     /locus_tag="VBC52_000591"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEO6882193.1"
                     /translation="MSHPKLESSSNKEIITEEVGLLKQLLDEATQKLIGSESFDKIEK
                     IVSLSLTDDYTGLKETISALSNEEMVIVSRYFSILPLLINISEDVDLAYEINYKNNLN
                     QDYLGKLSTTIDVVAGHENAKDILEHVNVVPVLTAHPTQVQRKTVLELTSKIHDLLRK
                     YRDVKAGIVNQEKWYADLRRYIGIIMQTDTIREKKLKVKNEITNVMEYYNRSLIKAVT
                     KLTAEYKALAAKKGIHLENPKPLTMGMWIGGDRDGNPFVTAETLRLSAMVQSEVIINH
                     YIEQLNELYRNMSLSINLTEVSPELVTLANQSQDNSVYRENEPYRKAFNFIQDKLVQT
                     LLNLKVGSSPKEKFVSRQESSDIVGRYIKSHIAQVASDIQTEELPAYATAEEFKQDLL
                     LVKQSLVQYGQDSLVDGELACLIQAVDIFGFYLATIDMRQDSSINEACVAELLKSANI
                     VDDYSSLSEEEKCQLLLKELTEDPRTLSSTHAPKSELLQKELAIFQTARELKDQLGED
                     IINQHIISHTESVSDMFELAIMLKEVGLIDANQARIQIVPLFETIEDLDNSRDIMTQY
                     LHYELVKKWIATNNNYQEIMLGYSDSNKDGGYLSSGWTLYKAQNELTKIGEENGIKIT
                     FFHGRGGTVGRGGGPSYEAITSQPFGSIKDRIRLTEQGEIIENKYGNQDAAYYNLEML
                     ISASIDRMVTRMITNPNEIDNFRETMDGIVSESNAVYRNLVFDNPYFYDYFFEASPIK
                     EVSSLNIGSRPAARKTITEISGLRAIPWVFSWSQNRIMFPGWYGVGSAFKHFIEQDEA
                     NLAKLQTMYQKWPFFNSLLSNVDMVLSKSNMNIALQYAQLAGSKEVRDVFNIILNEWQ
                     LTKDMILAIEQHDNLLEENPMLHASLDYRLPYFNVLNYVQIELIKRLRSNQLDEDYEK
                     LIHITINGIATGLRNS"
     gene            3000..4799
                     /gene="pepF"
                     /locus_tag="VBC52_000592"
     CDS             3000..4799
                     /gene="pepF"
                     /locus_tag="VBC52_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO6882194.1"
                     /translation="MKLKKRSEFPENELWDLTALYKDRQDFLLAIEKALEDIKVFKKN
                     YEGKLNCVEDFTSALMEIEHIYIQMSHIDTYAFMPQTTDFSNEEFAQISQAGSDFATK
                     ANVLLSFFNTALANADIKILDSLENNPHFKATIRQAKIQKQHLLSPEVEKALTNLNEV
                     LNTPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVTYENYFQNHENAEIREKSFRSFSKG
                     LRKHQNAAAAAYLAKVKSEKLIADMRGYDSVFDYLLSEQEVDRSMFDRQIDLIMDEFG
                     PVAQRFLKHIADVNGIEKMTFADWKLDIDNELNPEVSINDAYDLVMKSVAPLGKEYSQ
                     EVERYQKERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSFFNTHMSTYYVEAPSTFNELLLSDYLENQFDTARQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGGTFGAEQLNAIMKEVLTQFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLNLKNNPNGAKEWLAFLKSGGSRTPLETALLIS
                     ADISTDKPLRDTINFLSNTVDQIINYSNLIEQG"
     gene            complement(4858..5247)
                     /locus_tag="VBC52_000593"
     CDS             complement(4858..5247)
                     /locus_tag="VBC52_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEO6882195.1"
                     /translation="MKKYIKLFLLTVFATTLVACGQPSTSNKTTTSSTLEVGKVELVV
                     KEDTNVLSEKVVYHKGDTVLDVLKANYKVKEKDGFITSIDGISQDETKGLYWMFKVNN
                     KLAPKAANQIKVKKNDKIEFYQEVYKK"
     gene            complement(5231..5755)
                     /locus_tag="VBC52_000594"
     CDS             complement(5231..5755)
                     /locus_tag="VBC52_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6882196.1"
                     /translation="MIKNSTLTKLTRVAILSALCVVLRYAFAPLPNIQPITAIFLITV
                     VLFDLKEGVATVTITMLVSSFLMGFGPWVFLQIISFTLILCLWKFLIYPLTKAVCFGK
                     ITEVVLQTFFAGGLGVVYGVIIDTCFAWLYHMPWWTYVLAGLSFNMAHALSTCLFYPL
                     LLPILRRFRNEKIH"
     gene            5911..6759
                     /locus_tag="VBC52_000595"
     CDS             5911..6759
                     /locus_tag="VBC52_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEO6882197.1"
                     /translation="MRRRIKPIVVAVFFSLFGLLLIIGHLHSTNTLKKELVEAKKTIP
                     SVKASKVPQKSTSSKDKEFVLKPIIDVSGWQLPKEIDYDTLSKNISGVVIRVFGGSKI
                     SKTNNAAYTTGIDKSFKTHIKEFQKRNIPVAVYSYALGSSVKEMKEEAQIFYKNAAPY
                     KPTFYWIDVEEETMSNMNKGVQAFRKELKRLGAKNVGIYIGTYFMTEQGISVKGFDAV
                     WIPTYGSDSGYYEAAPQTELKYDLHQYTSQGYLPGFNQPLDLNQIAVNKDKKKTYEKL
                     FGKVKE"
     gene            complement(6792..7409)
                     /locus_tag="VBC52_000596"
     CDS             complement(6792..7409)
                     /locus_tag="VBC52_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO6882198.1"
                     /translation="MEILIAGGSGFLGKQIIKAALTKGHKVAYLSRHEGKGDIFKDPR
                     LTYIRGDITEADKIHLEDRTFDILIDCIGAIKPNQLDELNVKATQKAVALCHKNQIPK
                     LVYISANSGYSAYIKSKRKAEQIIKASGLDYLFVRPDLMYGEERPLSIFQAKCIKLFS
                     HLPFLGIVVQKVFPTKVVIVAEAIVTTLRKKPTQKILSIEELNNK"
     gene            7704..8177
                     /locus_tag="VBC52_000597"
     CDS             7704..8177
                     /locus_tag="VBC52_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEO6882199.1"
                     /translation="MSKKNKIKKTLVDQILDKAKIEHDSLQLDALQGDLPNGIQKQDI
                     FKTLALIGDKTGPIIGILPLTEHLSEKKLAKISGNKKVQMIPQKDLQKITGYIHGANN
                     PIGIRQKHNYPIFIDTIALEKQELIVSAGEIGRSIRINSEVLADFVNAKFADIKE"
     gene            8199..8840
                     /locus_tag="VBC52_000598"
     CDS             8199..8840
                     /locus_tag="VBC52_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO6882200.1"
                     /translation="MKFYLVRHGKTQWNLEGRFQGANGDSPLLEEAIEELEELGQYLS
                     SIHFDAVYSSDLGRARDTVNILNDANSCPKEIHYTPQLREWALGTLEGCKIATMQAIY
                     PRQMTAFYQNPLQFKHDMFGAESLYQTTHRVESFLRSLASKNYDKVLIVGHGANLTAS
                     IRSLLGYQYGSLHYKDKLDNASLTIIETHDFKDFNCLTWNDKSYLRQEVKMTH"
     gene            complement(8876..9778)
                     /locus_tag="VBC52_000599"
     CDS             complement(8876..9778)
                     /locus_tag="VBC52_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6882201.1"
                     /translation="MITSIVFDVDDTIYDQQAPYRIAVEKCFPDFDMSAINQAYIRFR
                     HYSDIGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEATGIYFQEIYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVKKLGLYDYVDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPSTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRSLKPGIKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDINDKSNPVLEMGLNNGLMMAAERLLESNMS
                     VDKVVILLRLTAKQEKVLRMKYAR"
     gene            9953..11443
                     /gene="lysS"
                     /locus_tag="VBC52_000600"
     CDS             9953..11443
                     /gene="lysS"
                     /locus_tag="VBC52_000600"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEO6882202.1"
                     /translation="MSNQHIEELNDQQIVRREKMAALTEQGIDPFGKRFERTATSGQL
                     NEKYADKSKEDLHDIEETATIAGRLMTKRGKGKVGFAHIQDREGQIQIYVRKDSVGEE
                     NYEIFKKADLGDFLGVEGQVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDIETI
                     YRKRHLDLISNRDSFDRFVTRSKIISEIRRFMDSNGFLEVETPVLHNEAGGASARPFI
                     THHNAQDIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEAYQ
                     AYADYQDIMDLTEGIIQHVTKTVKGDGPINYQGTEIKINEPFKRVHMVDAVKEITGID
                     FWKEMTLEEAQALAQEKNVPLEKHFTTVGHIINAFFEEFVEDTLIQPTFVFGHPVEVS
                     PLAKKNDTDPRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQASAKELGDDEATG
                     VDYDYVEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(11518..11988)
                     /locus_tag="VBC52_000601"
     CDS             complement(11518..11988)
                     /locus_tag="VBC52_000601"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEO6882203.1"
                     /translation="MTIIEGQLVANEMKIGIVVSRFNELITSKLLSGAVDGLLRHGVS
                     EEDIDIVWVPGAFEIPYMARKMALYKDYDAIICLGVVIKGSTDHYDYVCNEVTKGIGH
                     LNSQSDIPHIFGVLTTDNIEQAIERAGTKAGNKGYDCALSAIEMVNLDKKLRER"
     gene            complement(12003..13196)
                     /locus_tag="VBC52_000602"
     CDS             complement(12003..13196)
                     /locus_tag="VBC52_000602"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEO6882204.1"
                     /translation="MAFSPIKKLLQDIKSGKMVVLMDDENRENEGDLICAAEMVTKES
                     INFMAKFGKGLICLPLSNYYAEKLELAQMASHNTDNHETAFTISIDHLSTSTGISAED
                     RALTAKMVANDSSKAKDFRRPGHLFPLLAKEGGVLARNGHTEATVDLCRLAGLKECGL
                     CCEIMAEDGSMMRKDELLAFAQKHDLAIATIKQLQDYRRQEEGGVVREIEIQLPTQFG
                     HFTAYGYSEVVANKEHVALVKGDISSGEDVLCRLHSECLTGDVFHSLRCDCGEQLANA
                     LQQIEAEGRGVLLYMRQEGRGIGLINKLKAYHLQEEGLDTLEANLALGFEGDERDYGV
                     SAQLLKDLGINSINLLTNNPDKIQQLEAEGICVKNRVPLQVAATAYDLNYLKTKKEKM
                     GHLLD"
     gene            complement(13214..13864)
                     /locus_tag="VBC52_000603"
     CDS             complement(13214..13864)
                     /locus_tag="VBC52_000603"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEO6882205.1"
                     /translation="MFTGIIEEMGQVSRIRNGIKSQQLSIDAPKLVPLLRKGDSVAVN
                     GVCLTVLDKSETAFIADVMPESMMRTSLAALRLHSKVNLELALRSDSRLGGHFVLGHV
                     DGVGKIEKIQKDDIAVRFSIDAPPSIMSYIIEKGSVALDGISLTVVSFTEHSFEVSVI
                     PHTMAQTNLSLKKVGDLLNIEVDVLGKYAEKFLAPTNRTNHTSSVMDWSFLSENGY"
     gene            complement(13845..14954)
                     /gene="ribD"
                     /locus_tag="VBC52_000604"
     CDS             complement(13845..14954)
                     /gene="ribD"
                     /locus_tag="VBC52_000604"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEO6882206.1"
                     /translation="MMKKEDYMALALKEAEKGMGFVAPNPLVGAVIVKDDRIISKGYH
                     KRFGDLHAERQAIKNADEDISGSTLYVTLEPCCHVGKQPPCTEALIKSGIKKVVVGSL
                     DPNPLVSGKGIALLRKEGLNVEVGILREECDALNERFIFYMTYKQPFVYLKYAMTLDG
                     KIATKTGDSKWISNEHSRQSVQKLRQKCSAIMVGINTVLADNPRLTCRIPKGEALVRI
                     VCDSQLKIPLDSYLVKSAKTIPTWIATCSDNLAQQQTLKEMGCRLIKVPRKDGKLDLK
                     VLMTILGQEGIDSLLIEGGSSLHFSALKAGIVNRLIVFIAPKIIGGLKAKTAISGEGL
                     DWLNQAFRVKDIELSRMDSDVVIEGKVEHYVYRNY"
     regulatory      complement(15148..15262)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            15683..17023
                     /locus_tag="VBC52_000605"
     CDS             15683..17023
                     /locus_tag="VBC52_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001073016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEO6882207.1"
                     /translation="MNQNHSVKRSLSEVNQSVEVPHNSSFWNTLRAFLGPGALVAVGY
                     MDPGNWITSVIGGATYRYLLLFVVLVSSLMAMQLQQMAGKLGIVTRQDLAQATASRLP
                     KPLRYLLFIIIELALIATDLAEVIGSAIALHLLFGWPLLLSIMITILDVFLLLLLMKL
                     GVQKIEAFVSVLILTILIIFTYLVVLSQPDLDAMFKGFLPHHELFNISHEGKNSPLTL
                     ALGIIGATVMPHNLYLHSSLSQTRRVDYHNKSSIKKAVRFMTLDSNIQLSLAFVVNSL
                     LLVLGASLFYGHANDISAFSQMYLALSDKTITGAVASSFLSTLFAVALLASGQNSTIT
                     GTLTGQIVMEGFLHFKLPQWLIRLCTRLLTLLPIFVIALLVGGEENTLDQLIVYSQVF
                     LSLALPFSIFPLIYFTSQKSIMGEHANAKWNTYLAYLVAIILTLLNLKLIMDLL"
     gene            complement(17125..17922)
                     /locus_tag="VBC52_000606"
     CDS             complement(17125..17922)
                     /locus_tag="VBC52_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882208.1"
                     /translation="MIRHSFKSKVLLLVFILTIIVNYLSATGFLTGNSQKSLSDRYQT
                     LLTPAPLAFSIWSVIYLLTFLVILRAIFSKSQSYQDNFASIFPYFLGLLLVNNIWTVF
                     FTSNLIGLSTIIIFAYCILLVIIIKILSKNKSKLLLRITFGIHAGWLLVASLVNLAVY
                     LVKIDFNYPLPKVYIAIIALIFITVLSLYLARVLQNAYLILSVFWAWLMVFKAHLEGN
                     FQHLYFSIQILSLIAAIILGVSFFIIGYYNVKERYKQKNTSPYSRVL"
     gene            complement(18046..18258)
                     /locus_tag="VBC52_000607"
     CDS             complement(18046..18258)
                     /locus_tag="VBC52_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEO6882209.1"
                     /translation="MSEFTFEIVEKLLVLSENEKGWTKELNRVSFNGAPAKFDLRTWS
                     PDHTKMGKGITLSNEEFKVILDAFRK"
     gene            complement(18385..19671)
                     /locus_tag="VBC52_000608"
     CDS             complement(18385..19671)
                     /locus_tag="VBC52_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO6882210.1"
                     /translation="MSNVKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGINYAHARDAKVYVAANMVTHEGNELGAGPWFRELRDMGLDAVIVSDP
                     ALIVICATEAPGLEIHLSTQASSTNYETFEFWKEMGLTRVVLAREVTMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGQERQSL
                     KGEIPEPFSMSAVDMCMIEHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFEAIKEDLIDELWKVAQRELATGFYYHTPTENEQLFGARRKIPQYKFVGEVV
                     SFDNAKMEATIRQRNVIMEGDRVEFYGPGFRHFECFIDGLRDAEGNKIDRAPNPMELL
                     TITLPNPVKKGDMIRACKEGLVNLYQNDGTSKTVRA"
     gene            complement(19801..20727)
                     /locus_tag="VBC52_000609"
     CDS             complement(19801..20727)
                     /locus_tag="VBC52_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO6882211.1"
                     /translation="MEKIILTATAESIEQVKQLLAIGIDRIYVGEENYGLRLPHSFSD
                     DELREIAKLVHDAGKELTVACNALMHQEMMDNIKPFLELMKEINVDYLVVGDAGVFYI
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVETVLAREIPSEELFKMSENLEFPAE
                     ILVYGASVIHHSKRPLLQNYYNFTHITDEKTRERGLFLAEPGDPESHYSIYEDKHGTH
                     IFINNDINMMTKVTELVEHHFTHWKLDGIYCPGDNFVAIAEIFVETARLIENGTFTQA
                     QAFLFDERIRKLHPKGRGLDTGFYDFDPSTVK"
     gene            21067..21342
                     /locus_tag="VBC52_000610"
     CDS             21067..21342
                     /locus_tag="VBC52_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEO6882212.1"
                     /translation="MSSPLKQEQTHESKTEQAQPKFQVFQEFDHSAKLRELVFFARIA
                     LFSITTVLISFLLLALSLTPVWSFLLAMLASLAVTSAVSSFIWSLRQ"
     gene            complement(21383..21814)
                     /locus_tag="VBC52_000611"
     CDS             complement(21383..21814)
                     /locus_tag="VBC52_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO6882213.1"
                     /translation="MSKLFKTLVISAASGAAAAYFLTTKKGKELRKNAEKFYGEYKEN
                     PEEYHQIAKDKASEYSNLAVDTFKDYKGKFESGELTTEDIVSAVKEKSGEVVDFANDF
                     VNQAKSKFSDEDTAKKEDKAPETKVEDIVIDYKENTEDKEK"
     gene            complement(21811..22209)
                     /locus_tag="VBC52_000612"
     CDS             complement(21811..22209)
                     /locus_tag="VBC52_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEO6882214.1"
                     /translation="MIEIAVLIIAIAFVVLVLGILFVLKKVSETIEETKQTIKVLTSD
                     VNVTLYQTNEILAKANVLVDDVNGKVSTIDPLFVAIADLSESVSDLNLQARHIGQKAS
                     SATSSVTKAGSALAIGKAASKIFRKKGDKK"
     gene            complement(22224..22997)
                     /locus_tag="VBC52_000613"
     CDS             complement(22224..22997)
                     /locus_tag="VBC52_000613"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEO6882215.1"
                     /translation="MINPVAIRLGPFSIRWYAICIVSGMLLAVYLAMKEAPRKNIKSD
                     DILDFILMAFPLSIVGARIYYVIFEWAYYSKHPVEIIAIWNGGIAIYGGLITGAILLV
                     IFSYRRLINPIDFLDIAAPGVMIAQAIGRWGNFINQEAYGRAVKNLNYVPNFIKNQMY
                     IDGAYRVPTFLYESLWNFLGFVIIMSIRHRPRTLKQGEVACFYLVWYGCGRFIIEGMR
                     TDSLYLAGLRVSQWLSVILVIIGIVMIIYRRREQHISYY"
     gene            complement(22990..23925)
                     /locus_tag="VBC52_000614"
     CDS             complement(22990..23925)
                     /locus_tag="VBC52_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEO6882216.1"
                     /translation="MAVTVQMLVDRLKLNVIYGDEHLLSKRITTADISRPGLEMTGYF
                     DYYAPERLQLVGMKEWSYLMAMTGHNRYQVLREMFQKETPAIVVARDLEIPEEMYEAA
                     KDTGIAILQSKAPTSRLSGEVSWYLDSCLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDIMSLYGAS
                     AVKDSSQVQLAIYLENFETGKVFDRLGNGNEEIELSGVKVPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKQMGFDATQTFEDRLTHLISQNEVNDD"
     gene            complement(24153..24416)
                     /locus_tag="VBC52_000615"
     CDS             complement(24153..24416)
                     /locus_tag="VBC52_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEO6882217.1"
                     /translation="MKSSFYKQRKGKLVCGVVAGLADKYNWDLALSRVLIALILYFTK
                     FGLLLYILLAVFLPYKEDIIETRRQGPRRRKDAEPVDDDGWFW"
     gene            complement(24497..24955)
                     /locus_tag="VBC52_000616"
     CDS             complement(24497..24955)
                     /locus_tag="VBC52_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEO6882218.1"
                     /translation="MDLVLSSQTDLTNYVKKVSIEDFGCQFLHTAHWNNRLRTTGGRF
                     FPNDRHLDFNPKIYREFGIDIFRKIVRHELCHYHLYIQGKGYQHRDKAFKELLEKVDG
                     LRYTPRVTAVKVNYHHYSCQSCGQVYRRRRRVNLRKFACGYCHGRLIESF"
     gene            complement(24918..27080)
                     /locus_tag="VBC52_000617"
     CDS             complement(24918..27080)
                     /locus_tag="VBC52_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEO6882219.1"
                     /translation="MENLNITQIAIDLGIKASQIEKVLELTDEGNTIPFIARYRKEMT
                     GNLDEVQIKSIIDLDKSMTALSDRKTTVLAKIEEQGKLTQELKKAIEEATKLADVEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNKDNLEEEAQNYLTDGFETTTKALSGAVD
                     ILIEAFSEDNKLRSWTYNEIWNYSSITAVVKDESLDEKQVFKIYYDFSEKISKLHGYQ
                     VLALNRGEKMGVLKVNFEHNLEKMFRFFAVRFKETSQYIDDLIVQTVKKKIVPAMERR
                     IRTELSEGAEDGAISLFSENLRNLLLVSPLKGKMVLGFDPAFRTGAKLAVVDQTGKLM
                     TTQVIYPVPPANQAKIEQSKIELAKLIKEFNIEIIAIGNGTASRESEAFVAEVLQDFP
                     DVSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLAENLDFVVETVVNQVGVNVNTASPALLAHVSGLNKTISENIVKYR
                     EENGQIKSRAEIKKVPRLGAKAFEQAAGFLRIPNAKNFLDNTGVHPESYEAVKKLLDQ
                     LTIKELDDLAKEKLQNLDLIATAESIGVGQETLKDIIEDLLKPGRDLRDDFEAPVLRH
                     DVLDVSDLKVGQELQGTVRNVVDFGAFVDIGVHEDGLIHQSRLIKRKRDKKTRKMPPL
                     QHPSKYLSVGDIVTVWVVEVDAERSRIGLSLIKPDGLD"
     gene            complement(27185..28228)
                     /locus_tag="VBC52_000618"
     CDS             complement(27185..28228)
                     /locus_tag="VBC52_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEO6882220.1"
                     /translation="MIELGITTFGETTILEETNQSYSHPERIRQLVAEIELADQVGLD
                     VYGIGEHHREDFAVSAPEIVLAAGAVRTNNIRLSSAVTILSSNDPIRVYQQFSTIDAL
                     SNGRAEIMAGRGSFIESFPLFGYDLADYDDLFNEKMDMLLAINSATNLDWKGHLTQTV
                     NERPIYPRALQRQLPIWVATGGNVDSTIRIAEQGLPIVYATIGGNPKAFRQLVHIYKE
                     VGSRNGHKPEQLKVAAHSWGWIEEDNQTAIDRYFFPTKQTVDNIAKGRPHWSEMTKEQ
                     YLRSVGPEGAIFVGSPEVVAHKIIGLVEALELDRFMLHLPVGSMPHKDVLNAIKLYGK
                     EVAPIVRKYFEKN"
     gene            28361..28489
                     /locus_tag="VBC52_000619"
     CDS             28361..28489
                     /locus_tag="VBC52_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6882221.1"
                     /translation="MAITHKRQDDLESMFASFAKVPKPKKVDSDSKPEQKDDSQKD"
     gene            28495..29397
                     /locus_tag="VBC52_000620"
     CDS             28495..29397
                     /locus_tag="VBC52_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO6882222.1"
                     /translation="MDIFNQLPDSVLQWFAIFISIIIEALPFVLLGTILSGIIEVFIT
                     PDIVNKFLPKNKFLRVLFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSIRFLILRFVGATIVAIALGVMLAFLVDDNILKEDAKPTHFH
                     DYSDKKWYQKIFLALAHAIDEFFDTGRYLVFGTLIASAMQIYLPTRVLTTIGHSPITA
                     ILVMMLLAFILSLCSEADAFIGASLLSTFGIAPVMAFLLIGPMIDIKNLMMMVNSFKT
                     RFIVQFISVSSLIIIIYCLFVGVI"
     gene            29397..30209
                     /locus_tag="VBC52_000621"
     CDS             29397..30209
                     /locus_tag="VBC52_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEO6882223.1"
                     /translation="MIRFLILAGYFELSMYLKLSGKLNQYINTHYTYLAYISMVLSFI
                     LAIVQLIIWVKNMKMHSHLHGKIAKSTSPMILVFPVLVGLLVPTVSLDSTTVSAKGYN
                     FPLAAGSTGTVSQDGTRVQYLKPDTSTYFTSSAYEKEMQKELKKYKGSGTLTITTENY
                     MEVMELIYLYPEQFMDRQIQYTGFVYNEPKHEGYQFIFRFGIIHCIADSGVYGLLTTG
                     NQKSYPDNTWVTVRGTIKSEYNQLLQQNLPVLHIEESRQVSKPNNPYVYRVF"
     gene            complement(30246..31856)
                     /gene="ftsY"
                     /locus_tag="VBC52_000622"
     CDS             complement(30246..31856)
                     /gene="ftsY"
                     /locus_tag="VBC52_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEO6882224.1"
                     /translation="MGLFDRLFGHKKKDKEPEIEASESVVLEDEDSVIDKEEGSNFSK
                     ESTLNRTSEVPVAEDDSFLELERDTALSESHQPVTSEIHPLESEDTDEIPVKEDDSFL
                     ELEDRAKTKVADTSEVENVVPDSTTLSDNVSAKSEASFSDKEQLSDSQASDQFSETPL
                     QEEMSSGKTEVQTESEDTSAADAFLADYYAKRKAIEKEISSNSLSTDESEFSEAQEVL
                     SQSQADTIKAESQEEKYNRSLKKTRTGFSARLNAFLSNFRRVDEEFFEELEEMLILSD
                     VGVNVATQLTEDLRYEAKLENAKKSEDLKRVIVEKLVEIYEKDGIYNEAINFQEGLTV
                     MLFVGVNGVGKTTSIGKLAHQYKSQGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVVT
                     GEEKADPASVVFDGMEKAVAQGVDVLLIDTAGRLQNKENLMAELEKIGRIIKRVVPDA
                     PHETLLALDASTGQNALSQAKEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVK
                     FIGFGEKIDDIGEFNSEDFMRGLLEGIL"
     gene            complement(31856..32680)
                     /locus_tag="VBC52_000623"
     CDS             complement(31856..32680)
                     /locus_tag="VBC52_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6882225.1"
                     /translation="MSDIKILALDLDGTLFTTDKKVSEENKVALKAAREKGIKVVITT
                     GRPLKAIGNLLEDLELVSDEDYSITFNGGLVQQNTGKILAKTAMTRQEVEDIHEELYQ
                     VGLPTDILSEGTVYSIANKGHHSQYHLANPLLEFIEVDDLEQVPKDVVYNKIVSVIDA
                     TYLDQQIAKLPDRLKVDYEMFKSRDIILELMPKGVHKAVGLELLTKHLGLDSSQVMAM
                     GDEANDLSMLEWAGLGVAMANGIPEAKAIAKATTICNNDESGVAEAIGKYILSEEN"
     gene            complement(32680..33477)
                     /locus_tag="VBC52_000624"
     CDS             complement(32680..33477)
                     /locus_tag="VBC52_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6882226.1"
                     /translation="MIKLVATDMDGTFLDENGTYDKKRLANVLKKFKEQGIVFTAASG
                     RSLLSLEQLFADFRDQMAFIAENGSAAVLFNRLAYEQHLSREQYLDIIDHLSKSPYME
                     NNEYVLSGKDGAYILSDANPDYIEFITHYYDNLQKVSHFEDVDDIIFKVTANFTEETV
                     RQAEEWVNQAIPYTTAVTTGFKSIDIILSSVNKRNGLEHLCEQYGIRAEEVLSFGDNI
                     NDLEMLEWSGKAIATENARPEVKEIADCIIGHHNNQAVMAYLESMVD"
     gene            complement(33568..37107)
                     /gene="smc"
                     /locus_tag="VBC52_000625"
     CDS             complement(33568..37107)
                     /gene="smc"
                     /locus_tag="VBC52_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEO6882227.1"
                     /translation="MFLKEIEMQGFKSFADKTKVEFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSAKSLRGGKMPDVIFAGTENRKPLNYAQVSVTLDNSDHFIENIADEVRVER
                     RIFRNGDSEYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEAIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQSKLEQTQGNLDRLEDIIYELDMQVQPLEKQASIAKRFLVL
                     DEERQGLHLSILIEDILQHQSDLTTVEEKLLTVRKELATYYQQRQSLEDENQSLKQKR
                     HHLSEEIEAKQLALLDVTKLKSDLERQIDLIRLESNQKAEKKEEAGQRLAELEIKAKD
                     CSDQITQKNIELTTLSEKIAQIRSEIVSTESSLERFSTNPDQIIEKLREDFVTLMQEE
                     ADTSNALTALLADIENQKQASQAKSQEIQEVSKNLEVLKSNAKVALERFEAAKKNVRQ
                     LLSHYQDLGQTLQNLEGEYKNQQSILFDHLDEIKSKQARISSLESILKNHSNFYAGVK
                     SVLQAKDQLGGIIGAVSEHLSFDKHYQTALEIALGGSSQHIIVEDESAAKRSIAFLKK
                     NRQGRATFLPLTTIKPRELAQHYLSKLQSSQGFLGIASELVTYDQRLSNIFKNNLGLT
                     AIFDTVDNANVAARQLNYQVRLVTLDGTELRPGGSYSGGANRQNNTVFIKPELDNLKK
                     ELKQAQSKQLIQEKEVATLLEQLKEKQETLAQLKNDGEQARLEEQRADIEYQQLSEKL
                     ADLNKLYNGLQLSSGALEQTTSENEKNRLEKELEQFAIKKEELTTSIAQIKEDKDSIQ
                     EKVNNLTTLLSEAQLEERDLLNEQKFERANCTRLEITLSEIKRDISNLQTLLSHQDSQ
                     LDKEELPRIEKQLLQVNNRRENDEEKLVSLRFELEDCEAALDDLAASLAKEGQKNESL
                     IRQQAQLESQCEQLSQQLMIFSRQLSEDYQMTLDEAKVKANVLEDILMAREQLKSLQA
                     KIKALGPVNIDAIAQFEEVHERLTFLNTQRDDLVHAKNLLLETITDMDDEVKTRFKST
                     FEAIRHSFKETFVQMFGGGSADLILTEGDLLSAGVDISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKSSQFIVVTHRKGT
                     MSAADSIYGVTMQESGVSKIVSVKLKEAQEMTN"
     gene            complement(37115..37801)
                     /locus_tag="VBC52_000626"
     CDS             complement(37115..37801)
                     /locus_tag="VBC52_000626"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEO6882228.1"
                     /translation="MKELRSKLEKDYGIVFANQELLDTAFTHTSYANEHRLLNISHNE
                     RLEFLGDAVLQLLISQYLFTKYPQKAEGDLSKLRSMIVREESLAGFSRLCGFDHYIKL
                     GKGEEKSGGRNRDTILGDLFEAFLGALLLDKGVEVVHAFVNKVMIPHVEKGTYERVKD
                     YKTSLQELLQSHGDVKIDYQVTNESGPAHAKEFEVTVSVNQENLSQGIGRSKKAAEQD
                     AAKNALATLQ"
BASE COUNT        11868 a         7480 c         5820 g        12705 t
ORIGIN      
        1 ctgaatttcg taaacctgtt gcaataccat taatagtaat atgaatcaac ttctcatagt
       61 cttcatctag ttggttacta cgaagacgtt taatcaattc aatttgaaca taattcaaaa
      121 cattgaaata tggcaaacga taatctaaac ttgcatgtaa cattgggttt tcttctaata
      181 aattatcatg ctgttcaata gctaaaatca tatctttagt taactgccat tcattaagga
      241 tgatattaaa aacatctcga acttctttac ttccagctaa ctgagcatac tgaagcgcaa
      301 tattcatatt tgattttgat aaaaccatat ctacattaga taaaagagaa ttaaagaatg
      361 gccatttttg atacatggtt tgtaattttg ctaagtttgc ctcatcttgt tcaataaagt
      421 gcttgaaggc tgatccaaca ccataccatc ctggaaacat aatacgattt tgtgaccatg
      481 aaaataccca tggaattgca cgtaaacctg aaatttctgt aattgtttta cgtgctgctg
      541 gccgagaacc aatatttaaa cttgatacct ctttaattgg acttgcttca aagaaatagt
      601 catagaaata aggattatca aaaactaaat ttctataaac ggcattactt tcagacacaa
      661 taccatccat tgtctcacgg aaattatcaa tttcattagg gttagtaatc atacgtgtta
      721 ccatgcggtc tatggaagct gaaataagca tttcaaggtt ataataggct gcatcttgat
      781 taccatattt attctcaatg atttcacctt gctctgttag gcgaattcga tctttgattg
      841 aaccaaaagg ttgtgatgtg atagcttcat aagatggacc accaccacga ccaacagttc
      901 caccacgacc gtggaaaaat gtgattttaa taccgttttc ttcacctatt tttgttagct
      961 cattttgagc tttatataga gtccaaccag atgataaata gccaccatct ttattactat
     1021 ctgaataacc tagcattatt tcttgataat tgttgttagt agcaatccat tttttaacta
     1081 attcataatg tagatactgg gtcataatat cacgtgaatt atccaaatct tcgatggttt
     1141 caaataacgg tacaatctga atacgagctt gattggcatc aattagtcca acttctttta
     1201 acatgatagc taactcaaac atatccgaaa cgctctctgt gtgagaaatg atatgctggt
     1261 ttataatgtc ttcacccaat tgatctttta actcacgtgc tgtttggaaa atagccaatt
     1321 ctttttgaag taactcggat ttaggagcat gcgtagaaga taatgttcta ggatcttctg
     1381 ttaattcttt taagagaagt tgacattttt cttcttctga aagagaactg taatcatcta
     1441 caatattggc agacttcaat aattctgcta cacaagcttc attaatacta gaatcttgac
     1501 gcatatcaat agtagctaaa taaaatccaa agatatctac tgcttgaata agacaagcta
     1561 attcgccatc aacaagagaa tcttgtccat attgaactaa agattgcttc acaagtaaca
     1621 aatcttgttt aaactcctca gcagtagcgt atgctggtag ttcttctgtc tgtatgtcag
     1681 aagctacttg agcgatatgt gattttatat accttcctac aatatcagaa gattcttggc
     1741 gactaacaaa tttctctttt ggggaagaac caacttttaa atttagtagg gtttgtacca
     1801 gtttatcttg gataaaattg aaagctttac ggtaaggttc attttcccta tataccgaat
     1861 tatcttgtga ctggtttgcc aatgtaacaa gttcgggaga gacttcagtt agatttattg
     1921 aaagtgacat attacgataa agttcattta gctgctcaat ataatggtta ataatcactt
     1981 cactttgtac catagctgat aatcttagtg tctcagcagt tacaaaggga ttgccatctc
     2041 tatcaccacc aatccacatc cccatagtca gaggcttagg attctccaag tgaatacctt
     2101 ttttagctgc cagtgcctta tattctgctg ttaatttggt aactgcttta ataagagaac
     2161 ggttgtaata ttccattaca ttggttatct catttttgac tttcaatttt ttctcgcgaa
     2221 tagtgtctgt ttgcatgata atgccgatgt aacgacgcaa gtcagcatac catttttctt
     2281 gattcacgat tcctgccttg acatctctgt atttacgtaa taaatcatga atcttagacg
     2341 ttaattcgag caccgtttta cgctgcacct gagtaggatg agctgttagc acaggcacaa
     2401 cattgacgtg ttcaagaata tctttagcat tttcatggcc agctacaaca tcaatagtcg
     2461 ttgataactt tcctaggtaa tcttggttaa gattattttt ataattaatt tcatatgcta
     2521 aatcaacgtc ttctgaaata ttaattaaga gcggcaaaat agagaagtaa cgagaaacaa
     2581 taaccatctc ctcgttgctt aaagccgaaa tagtttcctt cagaccagta taatcgtcag
     2641 ttaatgataa tgaaactatt ttttcaatct tgtcaaatga ttcactacca atcaattttt
     2701 gcgttgcttc atccaaaagc tgcttcagta aaccgacctc ttcggtaatg atttctttat
     2761 tgctactact ttctaatttc gggtgggaca aaccatcact tcctctctta aatctcacta
     2821 ctatgatacc attttaaagt aaaaagtaaa ccattctagg atttttagat agaattttca
     2881 gatttttact tatttatgac agtaatcagt ttttcttgtt cgactttttt gatatactga
     2941 ggataattga gattaactga ttaattgtta attaacaact attattaaag gagtatcgca
     3001 tgaaacttaa aaaacgctct gaattccctg aaaatgaact ttgggatctg actgctctct
     3061 acaaagatcg tcaagatttt ttactggcta ttgaaaaagc tcttgaggat atcaaggttt
     3121 tcaaaaaaaa ttacgaggga aaacttaatt gtgttgagga ttttactagt gcccttatgg
     3181 aaatcgagca tatttatatc cagatgagtc acattgatac gtatgctttt atgcctcaaa
     3241 ctactgactt tagcaacgaa gagtttgcac aaatttctca agcaggatca gacttcgcaa
     3301 ctaaagctaa tgtactactt tctttcttta atactgccct agcaaatgca gatataaaga
     3361 ttttagattc tttagaaaat aaccctcatt tcaaagcaac tattcgccaa gctaaaattc
     3421 aaaagcaaca cttattaagt ccagaagtcg aaaaagcttt gacaaactta aatgaagtcc
     3481 taaatacacc atatgatatt tatactaaaa tgcgtgctgg cgattttgat atggaagatt
     3541 ttgaagttga cggaaaaacc tacaaaaata gttttgtaac ttatgaaaat tactttcaaa
     3601 atcacgaaaa tgcagagata cgtgaaaaat ccttccgttc attctcgaaa ggtctacgta
     3661 agcaccaaaa tgctgcagct gcggcttatc ttgcaaaagt caaatctgaa aagttaattg
     3721 ctgatatgcg tggttacgat agtgtttttg attatcttct ttccgaacaa gaagttgacc
     3781 gttcaatgtt tgacagacaa attgatctta tcatggatga atttggtcca gtagctcaac
     3841 gattcctcaa acatatcgcc gatgttaatg gtattgaaaa gatgactttt gcagattgga
     3901 aattagacat tgataacgaa cttaatcccg aagtaagtat caatgatgct tatgacttag
     3961 taatgaaatc agttgctcct ttaggaaaag aatattctca agaggttgag cgctatcaaa
     4021 aagagcgttg ggttgatttt gcagctaacg ctaataaaga ttctggagga tatgctgcgg
     4081 atccttacaa agttcatccc tatgtactca tgagctggac ggggcgtatg agcgatgtct
     4141 acacacttat tcatgagatt ggacattctg gtcaatttat cttctcagat aatcaccaaa
     4201 gttttttcaa tactcacatg tccacttact atgttgaagc tccatctact tttaacgaac
     4261 ttttacttag tgactactta gaaaaccaat ttgatacagc acgtcaaaaa cgctttgctc
     4321 ttgcacatcg cttaactgat acttacttcc acaatttcat tactcactta cttgaagctg
     4381 ctttccaacg taaagtttac actctaattg aggagggagg aacttttgga gcagaacaat
     4441 taaatgctat tatgaaggaa gtattaacac aattctgggg agatgctatt gaaatagatg
     4501 acgatgccgc tttgacatgg atgagacaag ctcattacta catggggctc tatagctata
     4561 cttactctgc tggattagta atctcgacag ccggctatct caatctcaaa aataatccaa
     4621 acggagcaaa agagtggtta gcattcttga aatctggtgg gagccgtacc cctttagaaa
     4681 cagctctatt aatcagtgct gatatttcta ctgataagcc tctgcgcgat actattaatt
     4741 tcttatctaa cactgtagac caaattatta attacagtaa tcttattgag caaggctaaa
     4801 ctacagcctt tcaaaaacat aaaaacaagt tggagatatc tccaacttgt ttttatctta
     4861 ttttttgtaa acttcctgat aaaattcaat tttgtcattc tttttaactt taatttggtt
     4921 tgctgcctta ggagctaatt tattattgac cttgaacatc caataaagcc ctttggtttc
     4981 atcttggctg atgccatcaa ttgaagtaat gaaaccatct ttttctttaa ctttatagtt
     5041 agctttaagc acatccaaaa cagtatcacc tttatggtaa acaacctttt cacttaacac
     5101 gttcgtatct tctttaacca ccaattcaac cttaccaact tctaacgttg agctagttgt
     5161 tgtcttattc gatgtagatg gttgcccgca agctactaaa gttgtagcga atactgttag
     5221 caagaataat ttaatgtatt ttttcattcc taaatctcct taaaatcggt aacaaaagag
     5281 gataaaataa acaagttgat aaagcgtgtg ccatattaaa actaagtcct gctaatacat
     5341 aagtccacca aggcatatga tataaccaag caaaacaggt atcaataatg acgccataaa
     5401 caacccctaa ccctcctgca aagaaagttt gaagtactac ctccgttatt ttaccaaaac
     5461 atacagcttt tgtcagcgga tatatcagaa atttccacaa gcataaaatt aaagtgaagg
     5521 aaatgatttg caaaaagacc catggcccaa agcccattaa aaaagaagaa actagcattg
     5581 ttatagttac agtagctaca ccttctttta aatcgaataa gacaactgta atcaaaaaaa
     5641 tggcagtaat tggctgaatg ttaggcagag gcgcaaaagc atatctcaac acaacacaca
     5701 aagcagataa tatagcgact ctagtcaatt tagttaaagt tgaattcttt atcatgtctt
     5761 taattatacc tcaaaactat gactaccgct taaaagtctt ttttattaca atttcatttc
     5821 taatgttaca taaacgacct cttttttaaa agaacgtaaa taagcctgta aactttgatt
     5881 ttttaaacct tttaagttac aatatcaata atgagaagaa gaattaaacc tattgttgtt
     5941 gcggtttttt tcagtttatt tggactttta ttaattattg gtcatttaca ttcgacaaat
     6001 actttgaaaa aagaattagt tgaagctaaa aagacaattc catccgtaaa agcttcaaaa
     6061 gtaccgcaaa aatcaacatc atcgaaagat aaagagtttg ttcttaaacc gattatcgat
     6121 gtctctggtt ggcaacttcc taaggagatt gattacgata cgctttcaaa aaatatttca
     6181 ggtgttgtta ttcgtgtctt tggtggatca aagatatcta agactaataa cgctgcttat
     6241 acaactggaa tcgataaatc gtttaagacc catatcaaag aatttcaaaa gcgaaatatc
     6301 ccagtagctg tctacagtta tgcacttggt tcaagtgtta aagaaatgaa agaagaggct
     6361 cagatatttt ataagaatgc agctccttac aaaccaactt tttattggat tgacgtagaa
     6421 gaggagacaa tgtctaacat gaataaaggt gtccaagcat tccgaaaaga attaaaaaga
     6481 cttggtgcta aaaatgttgg tatctacatt ggtacttact ttatgactga gcaaggcatc
     6541 tctgtaaaag gatttgacgc tgtttggatt ccaacttatg gtagcgattc tggatactat
     6601 gaagcggctc cgcaaactga acttaaatac gatttacacc aatacacctc tcaaggttat
     6661 ctaccaggat tcaatcaacc gcttgattta aatcaaattg cagttaataa agacaagaag
     6721 aaaacttatg agaaactttt tggaaaagta aaagagtaaa agctactgtt aacagtagct
     6781 tttttgataa actatttatt atttaattct tcaatagaaa ggattttttg ggttggtttt
     6841 ttcctaagcg tagtaacgat tgcttctgcc actatcacaa ccttagttgg aaagaccttt
     6901 tgtacaacaa tacctaagaa aggcaaatga ctaaataact ttatacactt ggcttggaaa
     6961 atcgagagag gtcgctcttc accatacatc aaatctggtc ttacaaaaag ataatccaga
     7021 ccgcttgctt tgattatctg ctctgccttc cttttacttt taatgtaagc tgaatagccg
     7081 ctgttggctg aaatataaac taactttggt atttgatttt tgtgacagag tgctactgct
     7141 ttttgggttg ctttaacgtt aagctcatct agttgattgg gcttaatcgc tccaatacag
     7201 tcaattaata tatcaaaagt tctgtcttct aaatgaatct tatcagcttc tgtaatatct
     7261 cccctaatgt aggttaatct aggatcctta aatatatcac ctttaccttc atgtcttgat
     7321 aagtaagcca ctttatgccc ttttgtaagc gctgctttta ttatctgctt tcctaaaaaa
     7381 ccactaccac ctgcaatcag tatttccata gttccctctc cttcattttg taaatttagt
     7441 atagaaattt ttatatgcct tgtcaaagta atcgtttaat acctgaccac ggtatgattt
     7501 caagtcatta catcaggaca cttcatattt agctatttat aaaacttcta atttaacatt
     7561 ccaaatagta ctgttgcgat atttattatc attattccct gaaatggtat tcaagcttat
     7621 ttctaagagt cagtagaaat ttactataat catctttaaa tgttataata aatatgaaac
     7681 aaacttatta gttaagggtc attatgagta aaaagaataa aatcaaaaaa actttagttg
     7741 atcaaatttt agataaagca aaaatcgaac atgatagtct acaattggat gctttacagg
     7801 gagatttacc caacggtatt caaaaacaag atatctttaa gacattagcg ctaatcggtg
     7861 ataaaaccgg tcccataata ggtatcttac ctctcaccga gcatttatcc gaaaaaaagt
     7921 tagctaaaat ttcaggaaac aaaaaggtac aaatgatacc tcaaaaagat ctccagaaaa
     7981 taaccggtta tattcacgga gcaaataatc caattggaat tcgccaaaaa cataattacc
     8041 ctatttttat cgatactatc gctttagaaa aacaagaact cattgtctct gcaggagaaa
     8101 ttggacgttc tattcgtatt aatagtgagg ttcttgcaga tttcgtaaat gctaaatttg
     8161 ccgatataaa ggaataataa ttaggattgg agaaaaatat gaaattttat cttgttagac
     8221 acggaaaaac acaatggaat ttagaggggc gtttccaagg tgctaacgga gactcacctc
     8281 tccttgaaga agctattgaa gaattagaag aacttggaca atacctatca tctattcatt
     8341 ttgatgctgt ttattctagc gacttaggtc gtgctagaga tactgttaat atcctcaatg
     8401 atgctaattc atgcccaaaa gaaatccact atactcctca acttcgtgaa tgggcattgg
     8461 gaactttaga aggttgtaaa attgcaacta tgcaagctat ctatcctcgt caaatgaccg
     8521 cattttatca aaatccctta caatttaaac atgacatgtt cggtgcagaa tctctttatc
     8581 agacaacgca tcgtgtagag agtttcttac gttctctagc ttcaaaaaat tacgacaagg
     8641 tccttattgt aggacatgga gcaaacctaa ctgcgtctat tcgttcctta ctgggttatc
     8701 aatatggtag tttacactac aaagataaac ttgataatgc cagcctcaca atcatagaaa
     8761 ctcatgattt taaagacttc aactgtctga cttggaacga taaatcttat ttaaggcaag
     8821 aggtcaaaat gacacactaa aataatccac tagattcatc tagtggatta tttaattatc
     8881 tagcatactt catgcgtaat actttttctt gttttgcagt taggcgcagt aaaataacaa
     8941 ctttgtcaac gctcatatta ctctcaagca gacgctctgc tgccatcatt aaaccattat
     9001 taagtcccat ttcaaggact ggattacttt tatcgttgat atcaaaaata aatttattat
     9061 caggtaagtc aaacaacact ttaacagcac cgaataattg ctcaaagtta tcaatagcaa
     9121 catcataaac tggtttaatt cccggtttta aagaacgtcc tctatggttg aaccacatag
     9181 aatgccaacc accattaaat gcacccataa tatcattatc atatgaatca cccacataaa
     9241 gtgtagttga agggttcata tcaaattgct ctgctgctaa attaaaaatc tccttttcag
     9301 gcttttgaaa accagttgct tgactaacta taacacgttt tggatcaaca tagtcataaa
     9361 gtcccaattt tttaactttt ttcaattgat gttccgtagg accatttgta ataattccca
     9421 ttggtacatt ttttgatttc aaaaagtcaa gtgtcatacg catctcatca agcattgtaa
     9481 tattttcaag ttcatgttcg tatatttctt gaaaataaat accagtagct tcgtcaattt
     9541 cacgataacc aaactctaaa agcgtttctt tgcatcgcca gaaacgaaaa tattctgttg
     9601 tccattcacc agccattact cgtggaaagc caatatctga ataatgacga aagcgaatat
     9661 aagcttggtt aatagcgctc atatcaaaat caggaaagca tttctccaca gcaatacggt
     9721 aaggtgcttg ttgatcgtaa atcgtatcat caacatcaaa aacaattgaa gtaatcatga
     9781 ttatcctttt ctttctgaat tttcaattgt taattatacc tttttctacc cttattttca
     9841 aatggtgaaa agtctattaa aaatgagaat aacaccagaa actcataaaa taaattatga
     9901 attatcagtg tttttgtggt acaataggat gaatactaac ttggaggaaa ttatgtctaa
     9961 ccaacatatt gaagaattaa atgaccaaca gattgttcgt cgtgaaaaaa tggctgcttt
    10021 gactgaacaa ggcattgatc cttttggtaa acgatttgaa cgtacagcaa catctggtca
    10081 attaaatgaa aaatacgctg acaaatcaaa agaagacttg cacgacattg aagaaactgc
    10141 tacaatcgct ggtcgtttaa tgactaagcg cggtaagggg aaagttggct ttgcgcatat
    10201 ccaagatcgc gaaggtcaaa ttcaaattta tgtccgtaaa gattctgttg gcgaagaaaa
    10261 ctacgaaatc tttaaaaaag ctgatttagg tgacttcctt ggtgttgaag ggcaagtaat
    10321 gcgtacagat atgggagaac tttctatcaa ggctactcat attactcacc tttcaaaagc
    10381 actccgtcca cttcctgaaa aattccacgg cctaactgat attgaaacaa tctatcgtaa
    10441 acgtcatctt gatttaatct caaatcgtga cagctttgac cgttttgtaa cacgctcaaa
    10501 aatcatttca gaaattcgtc gctttatgga tagcaatggt ttcttagagg ttgaaacacc
    10561 tgttcttcat aatgaagctg gtggtgcttc tgctcgccca tttatcacac atcacaatgc
    10621 acaagacatt gacatggttc ttcgtatcgc aactgaattg catctaaaac gtcttattgt
    10681 aggtggtatg gagcgtgttt atgaaatcgg acgtattttc cgtaatgaag ggatggatgc
    10741 aacccacaat ccagagttca catctattga agcctaccaa gcctatgctg actaccagga
    10801 tattatggac ttgacagaag gtattatcca gcatgttaca aaaactgtaa aaggtgatgg
    10861 accaattaat tatcaaggca cagaaattaa aattaatgaa ccattcaaac gtgttcatat
    10921 ggttgatgcg gttaaagaaa tcactggtat tgacttctgg aaagaaatga ctcttgaaga
    10981 agcacaagct ttggcacaag aaaaaaatgt tccgcttgaa aaacatttca caacagttgg
    11041 tcacatcatt aatgccttct tcgaagaatt tgttgaagac actttaattc aaccaacttt
    11101 tgtctttggt catccagttg aagtgtctcc acttgctaag aaaaatgata cagaccctcg
    11161 ttttacagac cgtttcgaac tctttatcat gactaaagaa tacgctaatg cttttacaga
    11221 attaaatgac ccaattgatc agctatctcg ctttgaagca caagcatctg ctaaagaact
    11281 tggcgatgat gaagcaactg gagttgatta cgattatgtt gaagcacttg aatatggtat
    11341 gccacctact ggtggactag gtattggtat cgaccgtctc tgtatgctgt taactgatac
    11401 aacaactatc cgagacgtcc tacttttccc tacaatgaaa taagaatata ttaaaaccca
    11461 tccttattta aggtgggttt taatattacc tctactgaac tttgttaggt ttctgattta
    11521 tcgctcacgc aattttttat caaggttcac catttcaata gccgaaagag cacaatcata
    11581 acctttattt cctgcttttg ttcctgccct ctcaatagct tgttcgatgt tatccgttgt
    11641 tagaacacca aagatatgag ggatatctga ctggctattt aaatgaccaa taccttttgt
    11701 cacctcatta caaacataat cataatgatc agtgctgcct ttaatgacta ccccaagaca
    11761 gataatagca tcataatctt tatataatgc catttttcta gccatatagg ggatttcaaa
    11821 agcaccaggt acccatacaa tatctatatc ttcttctgaa acaccatgtc tcagcagtcc
    11881 atctactgct cctgataata atttagatgt aatcaattca ttgaaacgtg agactacgat
    11941 tcctattttc atttcgtttg ccactaattg tccttcaata attgtcattt tttcttcctc
    12001 ctttaatcta aaagatgtcc cattttttct tttttagttt ttaaataatt aaggtcataa
    12061 gctgttgctg ccacctgtaa tggcactctg tttttgacgc aaattccttc tgcttcaagt
    12121 tgttggattt tatctggatt attggttagt aagtttatag aatttatacc taaatctttt
    12181 aaaagttgag cactaactcc atagtctctc tcatctcctt caaaacctaa agccaaatta
    12241 gcttctaaag tatctaagcc ttcttcttgc aaatgataag cttttaactt attaatcaaa
    12301 ccaattcctc gaccttcctg tctcatataa agaaggactc cacgaccttc agcctctatt
    12361 tgttggaggg cattagccag ttgttcccca caatcacatc tcaaggaatg aaagacatca
    12421 cctgtcaaac attctgagtg taaacgacat aaaacatctt caccactact aatatcccct
    12481 ttaacaaggg caacatgttc cttatttgcc acaacttcgc tatatccata agccgtaaaa
    12541 tgaccgaatt gcgttggtag ctgaatctca atttctctaa ccacccctcc ctcttcttga
    12601 cgtctatagt cttgcaattg ttttatggta gcgatagcta aatcgtgctt ttgagcaaat
    12661 gctaataact catcctttct catcatacta ccatcttcag ccattatttc acaacaaagt
    12721 ccacattcct ttaatccagc taaacgacat agatcaacgg ttgcttcagt atgtccattt
    12781 ctagctaaaa cacctccttc cttggctaac aagggaaata agtgcccagg acgtctaaag
    12841 tctttagctt tgctactgtc gttagcaacc atcttagctg tcaaagctct atcttccgca
    12901 gaaatcccag tactagttga tagatgatca atagatatgg taaaagctgt ttcatgatta
    12961 tcagtattat ggctagccat ttgagctaat tctaactttt cagcatagta attagacaga
    13021 ggtaagcaaa ttaatccttt cccaaattta gccataaaat taatcgactc ttttgtcacc
    13081 atttcagccg cacaaataag gtctccttca ttctctctat tttcgtcatc catcaacact
    13141 accattttcc ctgatttgat gtcttgtaaa agttttttta ttggactaaa ggccattagc
    13201 tttctcctct ctcttagtag ccattttctg ataaaaaaga ccaatccatt accgatgaag
    13261 tgtgattagt tctattagtg ggtgctaaaa atttctcagc atatttgcct aaaacatcca
    13321 cttcgatatt taataaatcc ccaacctttt ttaaggatag attagtttga gccatcgtgt
    13381 ggggaatgac cgaaacttca aaagaatgct ctgtaaatga taccactgtt aagctaatcc
    13441 catctagagc caccgaacct ttctctataa tataagacat aatactaggt ggagcatcaa
    13501 ttgaaaatct gacagcaata tcatcttttt ggattttttc tatttttcca accccatcca
    13561 catgtccaag aacaaaatgc ccccctaacc ttgagtcact tctgagtgct aattctaagt
    13621 taactttaga atgaagtcgt aaagcagcta agcttgttcg catcatactt tctggcatga
    13681 cgtctgctat aaaagctgtt tcacttttgt caagaaccgt caaacaaaca ccattaacag
    13741 ctacgctatc cccttttctt aataaaggga ctagtttggg agcatcaata cttaattgtt
    13801 gggactttat gccattccga atacgactga cttgacccat ttcctcaata attcctgtaa
    13861 acataatgtt ctacctttcc ttcaataaca acgtcagaat ccatacgact aagttcaata
    13921 tcttttactc tgaaagcttg gttaagccaa tccaaacctt ctccagaaat agctgttttt
    13981 gctttcagac ctcctattat tttgggtgca ataaaaacaa ttagtctgtt aacaatacca
    14041 gcttttaaag ctgaaaaatg taaactactt cctccttcaa taagaaggct atcaatccct
    14101 tcttgtccta aaatagtcat taatactttc aaatccagct ttccatcttt tcttgggacc
    14161 ttaatcaaac ggcaacccat ttcttttaat gtttgttgct gagcaagatt atctgaacat
    14221 gttgcaatcc atgttggtat tgttttagcg ctcttaacta ggtaagagtc taaaggtatc
    14281 ttcaactgac tatcacatac gatcctcaca agcgcttcac ctttaggaat acggcaagtt
    14341 aaacgtggat tatcagctaa tacagtattg ataccgacca taatagcact acatttttgc
    14401 ctcaatttct gaacagattg tcttgagtgc tcatttgata tccacttaga atcgcccgtc
    14461 tttgtagcta tcttcccatc taaagtcata gcatatttca aataaacaaa aggctgctta
    14521 taggtcatgt aaaaaataaa tctttcattc agagcatcgc attcttctct taagatacca
    14581 acttcaacat ttaaaccttc ttttctcaac aaggcaatcc cttttcctga aactaaagga
    14641 tttggatcta aacttccaac tactactttt ttgataccag atttaataag agcttcagta
    14701 caaggtggtt gcttaccaac atgacagcat ggttctaaag tcacgtacag agtacttcca
    14761 gagatatcct catcagcatt tttaatagcc tgtctttccg catgtaaatc tccaaatcgt
    14821 ttatgataac ctttggaaat tatacgatca tctttaacaa taacagcacc tactaatggg
    14881 ttgggagcaa cgaaccccat acctttctca gcctctttca aggctaatgc catgtagtct
    14941 tcttttttca tcatttcctt tttccttcta aaataaaaac ccttagcttc taaaatagaa
    15001 aactaagggg agggatgaga agtcgttgat tatactaatt agcattatat tttaggtagc
    15061 acaaaaaaac taaagactcc ctatagaaaa ctatagggag tctctcatta atccagcaca
    15121 aataaactgc aagtagcaga caatcactga attcttctct catccagact ttactgtcgg
    15181 tactggaatc acaccagttc atgctcgaaa gctcgcggac tataccgccg gttaggattt
    15241 tcacctgacc ccgaagaaat tctatttgac agttatttta ctaaactcat ttttatttgt
    15301 caataaaaat aatgtgtttt ttattataaa ataataatta agtagctcat ctattcttat
    15361 ctctacaaga cagtatatct ataccataaa gcaatctttc gaactaatca aacaagaatc
    15421 gctagtatta ttggattata tttcttaaaa aattcactac agatatagtt tactccactt
    15481 aaataactta ggtaaatcta cggaactaaa aataatcgat agaaatctgt tattttgtta
    15541 tttcaaatgt aactgttaag aaaataattc tctaaatact aaacagcacc tcaattgatt
    15601 attaagtagc gttaattttt aattgttttt ctattttcta tttgttataa tagcttgaga
    15661 ttactagaaa gtatagtatt acatgaatca aaatcattcc gtcaaaagat ccttatccga
    15721 agttaatcag agcgttgagg ttcctcacaa tagctctttt tggaacactt taagagcttt
    15781 tcttggacct ggagctttgg ttgcagttgg ttatatggat ccaggtaact ggattactag
    15841 cgttattggt ggagctacct atcgttactt actattgttt gttgttttag tatcttcttt
    15901 gatggcgatg caattacaac aaatggctgg caagcttggc attgttacac gacaggactt
    15961 agcacaagca acagcttctc gactcccaaa accgcttcgt tacctgctat ttattatcat
    16021 tgagttagcc cttatcgcta ctgatttagc agaagtcatt ggctctgcca ttgctcttca
    16081 cttattattt ggctggcccc tgttactgtc tatcatgatt actattttgg atgtattctt
    16141 acttttactc ttaatgaagt taggcgtcca aaaaattgaa gcctttgttt ctgttctcat
    16201 attgacaatt ttgataatct ttacatattt agttgtttta tcacaacctg atttagatgc
    16261 tatgttcaaa ggatttttac cacaccatga gctttttaac atatcacatg aagggaaaaa
    16321 tagtccttta accctcgctc ttggcattat cggcgcaaca gtcatgccac ataatcttta
    16381 tttacattct tctttatctc agactagacg cgtggattat cataataaat cttcaataaa
    16441 aaaagctgta cgtttcatga ccttagattc taacattcag ctatcacttg catttgtcgt
    16501 gaattcgcta ctattagtct taggagctag tctattttat gggcatgcta atgatatcag
    16561 tgccttttct caaatgtatc ttgccctatc tgataaaact attactggtg cagtagccag
    16621 ttctttcctt tctacccttt ttgcagttgc cctactagca agtggtcaaa attcaacgat
    16681 tacaggtact ttaacaggac aaatcgttat ggaaggtttc cttcacttca aattacctca
    16741 atggttaata aggctttgta ctagattatt aactcttctt ccaatttttg ttatcgccct
    16801 actagttgga ggcgaagaaa atacgttaga tcaactaatc gtctattcac aagtttttct
    16861 ttccttagct ttaccatttt caatctttcc attgatttat ttcacgtcgc aaaaatcaat
    16921 tatgggagag catgctaatg ctaaatggaa tacctattta gcataccttg tcgccatcat
    16981 tttaacatta ctcaatttga aattaatcat ggatttactt taatacaata caaaaaagcc
    17041 ccactaatga actgaacctc tatcatgaga catacatcta atgatagagg aaagtacata
    17101 atgatggagc tttttgtatt tctttcaaag aacccgtgag taagggctag tatttttttg
    17161 cttgtacctc tccttgacat tataataacc gatgatgaaa aacgatacgc caaggataat
    17221 tgctgcaatc aaagataaaa tttggatact aaaatatagg tgttgaaaat ttccttctaa
    17281 atgtgcttta aaaaccatca accaagccca aaaaacggat aagattaagt aagcattttg
    17341 taatactcta gctaggtaaa gacttaaaac agttataaaa atcaaggcaa tgatagctat
    17401 atataccttt ggtaatgggt aattaaaatc aattttaact aagtagaccg ctaaattgac
    17461 caatgacgca actagcaacc atcctgcatg aattccaaat gttattctaa gcagtaattt
    17521 tgatttattt ttacttagta tcttgataat gataaccaga agaatacagt aagcaaaaat
    17581 tattattgtt gataagccaa tcaaattact cgtaaaaaat acagtccata tattattaac
    17641 taatagaaga cccaaaaagt atggaaaaat gctagcaaag ttatcttggt aagattgact
    17701 cttactgaat atagctctta atattactaa aaaggtcaat aaatagatga ctgaccaaat
    17761 tgaaaaggct aatggtgctg gtgtcaagag cgtttgataa cgatcagaca gactcttctg
    17821 actgttccct gttaaaaaac ctgttgcgga taaataattc actattattg ttaagataaa
    17881 gactaacaat aacaccttac ttttaaatga atgtctaatc atagaggcct ccttttacct
    17941 ccattataat tcttttagtt taaaaattct atttttttaa tcaatatgtt tactgaatac
    18001 tcgtttgtct cgacaaaaaa agtggttttt gaaaccactt tttatttatt tacgaaaagc
    18061 atctaaaatg actttaaatt cttcattaga taaagtgata cctttaccca tttttgtatg
    18121 atctggactc catgtacgca aatcaaattt agctggcgct ccgttgaagc tcacacgatt
    18181 caactctttt gtccaacctt tttcattttc agataggacc aataacttct caacaatttc
    18241 aaatgtaaat tctgacattt tatacctcca cttatactat tcgttatttt tatagctttt
    18301 tgttctattt aaatgaactt cttctatttt aacaattttt agtaatattg accatgtaaa
    18361 atgacaaaat agttataaaa gtatctaagc tcttacagtc ttgctagtac catcattttg
    18421 ataaaggttc actaatcctt ctttacaagc acgaatcata tccccttttt ttactggatt
    18481 tggtaatgtt atggttaata attccatcgg atttggagca cggtctattt tatttccttc
    18541 agcatcacgc agaccatcaa taaaacattc aaagtgacgg aagccaggac catagaattc
    18601 tacgcgatct ccttccataa taacattacg ctgacgaatt gtagcctcca ttttagcatt
    18661 gtcaaatgaa accacttccc caacaaattt gtattgagga attttacgac gagctccaaa
    18721 gagttgttca ttttcagttg gtgtatggta gtaaaaacct gttgctaatt cgcgttgtgc
    18781 aaccttccaa agttcatcaa tcaagtcttc tttaatagct tcaaaagctt ctggactttc
    18841 catataggca tctacagcag ctttatagca attagttact gtagaaacat aatgaatgga
    18901 tttcatacgt ccttctattt ttaaactatc tacaccattt tcaatcatat ctggaatatg
    18961 ctcaatcata cacatatcca cagctgacat tgagaaaggt tctggaatct cgccttttaa
    19021 cgattgacgt tcttgtccaa atggcatatc gtagaggtca tatttccaac gacatgactg
    19081 agagcaaccg ccacgattag catcacgatg gctcatatgg tttgatagaa cacatcgtcc
    19141 tgagtatgaa atacacatcg cgccatgaac aaatgcttct atctcaacat ctgtcctctt
    19201 cctgatttca gccaactctg ccatagtaac ctcacgtgct aatacgacac gagtaagccc
    19261 catctctttc caaaattcaa aagtttcata gttcgtggaa gaggcttgag ttgacaaatg
    19321 aatttctaag cctggtgctt ctgtagcaca aataacaata agagcaggat ctgaaacaat
    19381 gactgcatct agtcccatat cacgtaattc acgaaaccac ggacctgccc caagctcatt
    19441 accttcatga gtaaccatat tagccgctac ataaacttta gcatctcttg catgagcata
    19501 gtttattccc tcttgtaact cttccataga aaagttacca gctctacttc gaagaccata
    19561 cgcttgacct ccaacaaata cagcatctgc tccataatca atagcaactt ttaatttttc
    19621 tagtgttcct gcaggtgata aaacctcagg gcgttttttt acattagaca tcttatattt
    19681 ccttctattt tcaagataaa agaattattt tctatcttaa ttaacatacc aatcgatatg
    19741 cagttcttag tgtaattggc tacgctttga aatagaaaac tttcacgtag caaataaatc
    19801 ttattttact gttgatgggt caaaatcata aaatccagta tctaaaccac gtcctttagg
    19861 gtgcagtttt ctaattcgtt catcaaacaa gaaagcctga gcctgagtga atgttccatt
    19921 ttcaatcaaa cgagctgttt cgacaaaaat ttcagcaata gcaacaaaat tatctcctgg
    19981 acaataaata ccatctaatt tccagtgagt aaagtgatgt tcaactagtt cagtcacttt
    20041 agtcatcata ttgatatcat tgttaataaa aatatgagtc ccatgcttat cttcgtaaat
    20101 agaatagtga ctttctggat cacctggttc tgctaaaaaa agaccacgct cacgtgtttt
    20161 ttcatcagtg atatgagtaa aattataata gttttgcaat aacggacgct tagagtgatg
    20221 aattacagaa gccccataaa ctaaaatctc agcaggaaat tcaagattct cagacatctt
    20281 aaaaagttct tcagaaggta tttcacgtgc taaaaccgtt tctactgctc catgttgtcc
    20341 ccaaaaatta acttgacgac ttgatgtaac gaagacagaa gtgtcgtaga taagtttgaa
    20401 gttataacca tcacgcttgt tgatataaaa tactcctgca tcaccaacta ccaaataatc
    20461 cacattaatt tctttcatca actccaaaaa tggtttgata ttatccatca tctcttgatg
    20521 catgagtgca ttacaagcta ctgtcaattc tttaccagca tcatgtacta atttagcgat
    20581 ttcacgcaat tcatcgtctg aaaatgaatg tggtaaacga agaccgtaat tttcttcccc
    20641 tacatagatg cggtcaattc ctattgctag caattgcttt acttgttcaa tagattcagc
    20701 tgtcgctgtc aaaattattt tttccataat tagtattata acaaactttc agcattttta
    20761 agcatatttc ttgacaagaa taaataaaaa tattcttttc gaaaattata tcactttaga
    20821 ggttaagcac tatcagataa gaaaaaatat agtattgtaa tattatataa aattatatct
    20881 attttttctt ttccaattta aaaaagtaag gaaagtagta aaaaatactt ttagtattct
    20941 actactaata tgggcaatgt ttttgttttt ttgagaaaaa acctttattt taactcatag
    21001 tatcaaaaaa ctagtgacaa actctattat ttgtgatatg atatgctagt atcaaaggag
    21061 aaagctatgt catcaccact taagcaagag caaactcacg aaagcaaaac agagcaagct
    21121 caaccaaaat ttcaagtatt tcaagaattt gatcacagtg ctaagttgcg tgagctagtc
    21181 ttttttgctc gtatcgctct cttcagtata accactgtgc taatctcttt cttattacta
    21241 gcactaagtt taacacctgt ctggagtttc ttactagcga tgctggcaag tctagcagtt
    21301 acatctgcag tctcttcatt catttggtca ctcagacaat aaaaatctca taaaaaagaa
    21361 gctacttata gtagcttctt ttttattttt ctttatcttc tgtgttttct ttataatcaa
    21421 tgacaatatc ttctactttt gtttcaggag ccttatcttc ttttttagca gtatcctcgt
    21481 ctgagaattt tgatttagct tgattgacaa aatcattagc aaagtcaact acttctccgc
    21541 ttttttcctt aacggctgag acgatatcct ctgttgtcaa ttcacctgat tcaaatttac
    21601 ctttataatc tttaaaagta tcaacagcta aatttgaata ttcacttgct ttatctttag
    21661 ctatttgatg atattcttct ggattttctt tatattctcc atagaatttt tctgcatttt
    21721 tccttagctc ttttcctttt tttgttgtta aaaaataagc tgctgctgca cctgatgcag
    21781 cactgataac taaagtttta aataatttac tcattttttg tctccttttt tacgaaaaat
    21841 ttttgaagcc gcttttccaa tagctaaagc tgaacctgcc ttagtaacac ttgaggttgc
    21901 actacttgcc ttttgtccaa tatgtcttgc ctgtaggttt aaatcagata cagattctga
    21961 taagtcagca atagctacaa aaagaggatc aatagtagat actttaccat taacatcatc
    22021 aactaaaaca ttagctttgg ctaaaatttc attagtttgg taaagagtca cgttaacatc
    22081 gcttgttaat actttaatag tttgcttggt ttcctcaatc gtttctgata cttttttcag
    22141 aacgaaaagt attcctaaaa ctaaaacaac aaacgcaatg gcaataatta atacagcaat
    22201 ttctatcata atacctcctc tgtttaataa tatgaaatat gttgttcacg acgacgataa
    22261 ataatcatga caattccgat aataactaaa ataactgata accattgtga cacgcgcaaa
    22321 cccgccaaat aaaggctatc tgtcctcatc ccttcaataa tgaaccgtcc acatccatac
    22381 caaactaaat aaaaacatgc cacttcacct tgttttaaag tgcgcggtcg atgacgaata
    22441 gacatgatga tgacaaaacc taggaagttc caaagagact catataaaaa tgtaggtact
    22501 cggtaagctc catcaatata catttgattt tttataaagt ttggtacgta attcagattt
    22561 tttactgccc taccgtatgc ttcttgatta ataaaattcc cccacctacc tatggcttgg
    22621 gcaatcatca ctcctggagc agcaatatcc agaaaatcaa tgggattaat caagcgacga
    22681 tatgaaaaaa tcacaaggag aatggccccg gtaattaacc ctccgtaaat agcaataccg
    22741 ccattccaga tggcgataat ctctactggg tgcttagagt aataagccca ttcgaagata
    22801 acataataaa tacgagctcc aacaatggat agtggaaaag ccatgagaat gaaatctaga
    22861 atatcatccg acttaatgtt cttacgaggg gcctctttca ttgctaagta aactgctaac
    22921 aacataccag acactatgca aatagcatac cagcgaatag aaaaggggcc taatcgtatt
    22981 gctactggat taatcatcat tgacctcgtt ctgactaatc aaatgtgtta aacgatcctc
    23041 aaaagtctgg gtagcatcaa accccatttg tttagctctg tgattcatag ccgctgcttc
    23101 aataactact gacacgtttc ggcctgtttt aacaggaata cgtatccgag gaaccttaac
    23161 tccagataat tcgatctctt cattgccatt cccgagtcta tcaaaaactt ttccagtttc
    23221 aaagttttca aggtatatag ctaattgcac ttgcgatgaa tctttaaccg cactagctcc
    23281 atataggctc ataatatcaa tgattcccac acctctaatt tcaagtaaat gacgtaatat
    23341 ctcagcaggc tctccccaaa gcgtctcttc gtctttagca taaacatcaa cacgatcatc
    23401 tgctaccaaa cgatgtcctc gcttaactag ctctagtcct gtctcactct taccaatacc
    23461 ggaatcacct tgaatcagaa cacccatacc atagatatcc atcaagacac catgaacact
    23521 tgtcctctca gctaagcaag aatctaaata ccaagaaact tcacctgata aacgacttgt
    23581 aggggcctta ctttgaagaa tagcaatacc ggtatcttta gccgcctcat acatctcttc
    23641 tggaatttct aaatcacgtg ctacaacaat agctggtgtt tctttttgga acatttcacg
    23701 taacacttga taacgattat gtcctgtcat agccattaaa tatgaccact ctttcatacc
    23761 aacaagttgc aaacgctctg gtgcataata atcaaaatac cctgtcattt ctagaccagg
    23821 acgtgaaatg tcagctgttg taatccgctt agataataga tgttcatcac catagatgac
    23881 atttaacttt aagcggtcaa ctaacatttg aacagtaact gccatactta ctccttctta
    23941 taaaagtgaa tatccctatt gctataccct acaatttaaa gttgagcaaa ggtactaaac
    24001 actttcatta atttacaccc acattatatc atacttttta ctaaaagtaa gcgctaacgc
    24061 gtttttctga aaaaaagaag atattttgaa aaagtggtct aaataaagct aagaaaaaag
    24121 gagattcatc tagcaaatct cctttatatg aattaccaga accaaccgtc atcatcaaca
    24181 ggttcagcat ctttacgtct tctgggacct tgtcttcttg tttcaataat atcttcttta
    24241 tagggtaaga aaacagctaa taaaatatat agcaaaagac caaatttggt aaaatacaaa
    24301 atcagtgcaa tcaacacccg tgaaagagct aaatcccagt tatacttatc agctagaccg
    24361 gcgacaacac cgcagaccaa cttacctttt cgttgtttgt agaagcttga tttcatacct
    24421 atccttcttt ctatctactc tatattttaa ttataactac tttgacagta aaattaaata
    24481 ggccttacga ctgattttaa aaactctcta tcaagcgacc atgacaataa ccacaagcaa
    24541 acttgcgtaa attaacacgt ctcctacgtc ggtatacttg accgcaagat tggcaagagt
    24601 aatgatgata attcacttta acagccgtaa ctctaggggt atatcgtaag ccatcgactt
    24661 tttctagtaa ctctttaaaa gctttatccc tatgttgata tcctttacct tgtatataga
    24721 gatgataatg gcaaagctca tgtcttacaa tcttacgaaa aatatcaata ccaaactcac
    24781 gataaatttt gggattaaag tcaaggtgtc tatcgtttgg aaagaagcgt cctccagttg
    24841 ttcttaagcg attattccaa tgtgcagtat gtaaaaactg acatccaaaa tcttctatag
    24901 acaccttctt aacataatta gtcaagtccg tctggcttga taagactaag tccaattctg
    24961 ctacgctccg catctacctc tacaacccaa acagttacaa tatcaccaac agagagatat
    25021 ttgcttggat gttgtaaagg aggcattttt ctagtttttt tatcgcgttt tcgcttaata
    25081 aggcgagact ggtggattag accatcttca tgaacaccaa tatcaacaaa agcaccaaaa
    25141 tcaacgacgt ttcgaacagt tccttgcaat tcctgtccaa cttttaaatc actgacatct
    25201 aaaacatcat gtctcaaaac aggcgcttca aaatcatcac gcaaatcacg acctggtttc
    25261 aaaagatctt ctataatgtc ttttaatgtt tcttgaccta ccccaatact ttctgctgtt
    25321 gcaattaagt caaggttttg taacttctct ttagcaagat catctaattc tttgatagtc
    25381 agttggtcaa gcaacttttt aacagcttca taagactcag ggtgaacacc tgtattatcc
    25441 aagaaatttt tagcattagg aatacgtaag aatccagcag cttgctcaaa agcttttgct
    25501 cctaatctgg gcactttctt aatttcagca cgtgacttaa tttgcccatt ctcttcacga
    25561 tatttaacaa tattttcaga tattgtttta tttaaacctg aaacatgagc taacaaggct
    25621 ggacttgctg tattaacatt aacacctact tggttaacta cagtttcaac aacaaaatca
    25681 agattttctg ctaatttttt ttggctaaca tcatgttgat attgacccac accaatagat
    25741 tttggatcaa ttttgactag ttctgctaat gggtcttgca gacgtcttgc aattgagatg
    25801 gccgaacgtt tttcaaccgt taagtctgga aattcatgac gtgccaattc tgaggctgaa
    25861 taaacagagg caccactttc gttaacaatc acataggata cgtctggaaa atcttgtaaa
    25921 acttctgcaa caaatgcttc tgattcacga ctcgctgttc cattccctat agcaataatt
    25981 tctatattga attctttgat aagttttgct aactctattt ttgattgttc gatttttgct
    26041 tgattagctg gaggaactgg ataaatcact tgtgttgtca ttaattttcc agtttggtct
    26101 actactgcta attttgctcc tgtacgaaag gcagggtcaa aacctaacac cattttcccc
    26161 ttaagcggtg agactaacaa taagttacga agattctctg aaaacagtga aattgcacca
    26221 tcttctgctc cttcacttag ttctgtacgg atacggcgtt ccatagctgg aacgattttt
    26281 ttcttgacag tctgtacaat aaggtcatct atatattggc tagtttcttt aaatcgaaca
    26341 gcaaaaaagc gaaacatttt ttcaaggtta tgctcaaaat taaccttaag aacacccatt
    26401 ttctcaccac gattcaatgc taaaacttga tagccatgga gtttacttat tttttcagaa
    26461 aagtcatagt agattttgaa gacctgtttt tcatcaagtg actcatcttt aacaactgca
    26521 gtgattgagc tgtagttcca gatctcatta tatgtccatg aacgtaattt attatcctca
    26581 gaaaaagctt cgataagaat atctactgct ccagaaagtg ccttcgtagt tgtctcaaaa
    26641 ccatctgtaa gatagttttg agcttcttcc tcaaggttat ccttattttg taaaataaga
    26701 cgtgctaaag gaaaaagacc tgcttcacgt gcaatcgtag ctttcgtacg acgtttttct
    26761 ttataaggta ggtagagttc ctcaacatct gctagtttag ttgcttcttc aatagccttt
    26821 ttgagttctt gagtcaactt tccttgttct tcaattttgg ctaagaccgt agttttacga
    26881 tctgaaagag ctgtcatact cttatcaagg tcaataattg atttgatttg aacttcatcc
    26941 aaattacctg tcatttcttt acgataacgg gcaataaaag gaatagtatt tccttcatct
    27001 gttaattcta aaaccttttc gatttgtgac gctttaatac ctaaatcaat agcaatttgt
    27061 gtaatattta aattttccat aacgctttta ttatagcata tttttgactt acgaagagtt
    27121 ttaaaaagcg atttcaagat agcgttttta taatctaaca ataaaaaaac agaccagatc
    27181 tgctttaatt cttttcaaaa tattttcgaa caattggtgc tacttcctta ccataaagtt
    27241 tgatagcatt taggacgtct ttgtgaggca tagagccaac tggtagatgt aacataaaac
    27301 ggtctaactc aagtgcttca acaagtccta taattttatg tgcaaccact tcaggacttc
    27361 ctacaaaaat agcaccttct ggacctactg aacgtaaata ctgctctttg gtcatttcag
    27421 accaatgagg gcgtccctta gcaatattat cgacggtctg tttcgtaggg aaaaaataac
    27481 ggtcaatagc ggtttgatta tcctcttcaa tccatcccca agagtgagca gcaactttta
    27541 gttgttctgg tttgtgtcca ttacgactac caacttcttt ataaatatgg accaattgac
    27601 gaaatgcttt gggattccca ccaatagttg cataaacaat tggcaaacct tgttctgcaa
    27661 tacgaattgt agaatcaaca tttcctcctg ttgccaccca tattggtaac tgtctctgta
    27721 atgctcttgg ataaattggt cgctcattaa ctgtttgtgt caaatgacct ttccaatcga
    27781 gatttgtcgc tgagttaatt gctaacaaca tatccatttt ttcattaaat aaatcatcat
    27841 aatccgctaa atcgtatcca aacaatggaa aagactcaat aaaggaacca cgccctgcca
    27901 taatttctgc tctaccattt gaaagtgcgt caatcgttga aaattgctgg tagacgcgaa
    27961 taggatcatt ggaagagaga atcgttactg cactagataa acggatatta ttagttctaa
    28021 ccgctcctgc tgctaggaca atttcgggtg cagagaccgc aaaatcttca cgatggtgct
    28081 ctccaatacc atatacatct aaaccaactt gatcagctag ttcaatctca gcaactaatt
    28141 ggcgaatcct ctcaggatgt gaatagcttt ggtttgtttc ttcaaggatt gttgtctctc
    28201 cgaatgttgt aatacctaat tcaatcataa tcttacctca tcaatctact aataacattt
    28261 tatcactata tagtatcatt tccaatcgat ttgttttttt atgttaaaaa taagctaata
    28321 tagtataata ggttattata taaagaaaga ggtgccatta atggctatta cgcacaaacg
    28381 tcaagatgac ttagaaagta tgttcgctag ttttgctaaa gtaccaaaac ctaaaaaagt
    28441 tgattctgat tcaaaacctg aacagaaaga tgattctcag aaagattaag ttctatggat
    28501 atctttaatc aattaccgga tagtgtcctt caatggtttg ccattttcat ttccattatt
    28561 attgaggcac ttccttttgt tttgcttgga actattttat caggaattat tgaagtcttt
    28621 ataacacctg atatagtcaa taagttctta ccgaaaaata aatttctcag agtcttattt
    28681 ggaaccttcg ttggctttgt ctttccttct tgcgaatgtg gtatcattcc tattattaat
    28741 cgattccttg aaaagaaagt gccttcatac acggcagttc cgtttcttgc tactgcccct
    28801 attattaatc ctattgtttt atttgcgacc tactcagcat ttggaaattc catacgcttc
    28861 ctgatattac gctttgtagg tgctaccatt gtagccattg ctttaggagt catgcttgcc
    28921 tttttagtag atgataatat tctaaaagaa gacgctaaac ctactcattt tcatgattat
    28981 tctgataaga agtggtatca aaaaatcttt ctagccttag ctcatgccat cgatgaattt
    29041 tttgatacag gacgctatct agtatttggt actttaattg cttcagctat gcaaatttat
    29101 ttaccaactc gagttcttac tactatcgga catagcccta tcacagccat cttagttatg
    29161 atgttgcttg ctttcatctt gtcattgtgt agtgaagctg acgcttttat aggtgcttct
    29221 ttattatcaa cctttggaat cgcgccagtg atggctttcc ttttaattgg acccatgatt
    29281 gatattaaga atttaatgat gatggtaaat tcttttaaaa caaggtttat tgtccaattc
    29341 attagtgtat cttctcttat tattattatc tattgtttat ttgtgggggt aatataatga
    29401 ttcgatttct tattcttgct ggttattttg aattaagcat gtatttaaag ttatcaggta
    29461 aactaaatca gtatattaat actcattata cctatttggc ctatatttca atggtcctat
    29521 cttttatttt agcaattgta cagctaatta tttgggttaa aaatatgaaa atgcatagtc
    29581 acctacatgg taaaatagct aaatcaacaa gcccaatgat tttagtattt cccgttttag
    29641 taggtttgtt agtacctaca gttagtttag actcaaccac agtctctgcc aagggatata
    29701 atttccctct agcagccggc tctactggta ctgtaagtca agatgggaca cgcgttcaat
    29761 atttgaaacc agataccagc acttatttta cttcttcagc ctatgaaaaa gaaatgcaaa
    29821 aagagcttaa aaaatataaa ggctctggta cattaaccat cacaactgaa aattacatgg
    29881 aagttatgga actaatttat ctctatcccg agcaattcat ggatcgccaa atacaatata
    29941 ctgggtttgt ttataacgaa cccaaacacg aaggatatca atttattttc cgctttggaa
    30001 tcattcattg tattgccgat tcaggagttt atggtctact aacgactgga aatcaaaaaa
    30061 gttatccgga caatacttgg gtaactgtta gaggtacaat taaatccgag tataatcaat
    30121 tgttacaaca aaacttacca gtcttacaca ttgaagagtc tcgtcaagtt tctaaaccta
    30181 ataatcctta tgtttatcgt gtcttttaat ataaaaagaa ctttctaaat gaaagttctt
    30241 ttttcttata aaattccttc cagaaggcca cgcatgaaat cttctgaatt aaattcacca
    30301 atatcatcga ttttttcgcc gaaaccaatg aatttaacag gaatatctag ttcttgacga
    30361 atagccagta ccacaccacc cttagcagta ccatcaattt tagtcagaat aaggccagtt
    30421 aatggtgtaa ttttcgagaa ctctttagct tgacttaaag cattttgtcc tgtagatgca
    30481 tctaaagcaa gcaaagtttc gtggggagca tcaggtacga cacgcttaat aatacgtcca
    30541 attttttcca actctgccat aaggttttct ttattttgca gacgtcctgc tgtatcaatt
    30601 aacaagacat caacgccttg agcaacggct ttttccatgc catcaaagac aacgctagcc
    30661 ggatctgctt tttcttctcc agtgacaact ggtacatcaa cacggcgtcc ccattctact
    30721 agttgagcca cagcacctgc acggaaagta tcagctgcta ctaacataac ttttttccct
    30781 tgagacttgt attgatgagc taacttacct atagatgtcg tttttccaac accattaaca
    30841 cctacaaaaa gcattacggt taaaccttct tggaaattga tagcctcatt ataaatacca
    30901 tctttttcgt agatttcaac taatttttca acgataacac gttttaaatc ctctgatttc
    30961 ttggcattct ctaattttgc ttcataacga agatcttcgg ttaactgtgt tgcaacattt
    31021 accccaacgt cagataagat taacatttct tctaattctt caaagaactc ttcgtcaaca
    31081 cgacggaagt tagaaaggaa agcattaagg cgtgcactaa aacctgtgcg tgtttttttc
    31141 aggctacgat tatatttttc ctcttgactc tcagctttta tagtgtctgc ctgtgattgc
    31201 gataaaactt cctgtgcctc agaaaattct gactcatctg ttgacagcga attagacgag
    31261 atttctttct caattgcttt acgtttagca tagtaatctg ctaaaaaagc atcagccgca
    31321 gatgtatcct ctgactctgt ttgtacctca gtttttccac tagacatctc ttcttgaagg
    31381 ggagtttctg aaaactgatc tgaagcctga ctatcactta attgctcctt atcactaaaa
    31441 gatgcctcag atttagcaga aacattgtca gaaagagtcg tggaatcagg aactacattt
    31501 tctacctcag aagtatctgc tacctttgtc ttagccctat cttctagttc taaaaaacta
    31561 tcgtcttctt ttacaggaat ttcatcggta tcttctgact ctaaaggatg aatttcagag
    31621 gtaactggtt gatgtgattc tgacaatgct gtatctcttt ctaattctaa gaaagaatca
    31681 tcttctgcaa cagggacttc agaggttcga ttcaaagttg actccttcga aaagttgctg
    31741 ccctcctctt tatcaataac tgaatcttca tcttctaaaa caacagattc tgatgcttca
    31801 atttctggtt ctttatcttt tttcttatgt ccaaataggc ggtcaaataa tcccattaat
    31861 tttcctcact taaaatatat ttcccaattg cttcggcgac tccagactca tcgttattac
    31921 aaatagttgt cgcttttgct attgccttag cttctggaat accattagcc attgcaactc
    31981 ctaagcctgc ccactctagc attgataaat catttgcttc atctcccatt gccataactt
    32041 gactactatc taacccaagg tgttttgtca gtagttctaa gccgactgct ttatgaacac
    32101 ctttaggcat caattctaaa atgatatcac gtgatttaaa catttcatag tcaactttaa
    32161 gtctatcagg aagtttagca atttgttgat caagatatgt agcatcaata acactaacaa
    32221 ttttattata aactacatcc tttggcactt gctctaaatc atcaacttct ataaattcta
    32281 agagcgggtt ggctaaatga tattgagaat ggtgtccctt gttagcgata ctatataccg
    32341 ttccctcact taagatatct gttggcaaac ccacttgata caactcctca tggatatctt
    32401 ccacttcttg tcgtgtcatt gcagttttag ctaagatttt ccctgtattt tgttgaacta
    32461 aaccaccgtt aaaagtaatg ctgtaatctt catcagatac taactctagg tcctctaaca
    32521 aattaccaat tgcttttaaa ggacgccctg ttgtaataac aacttttata cctttttctc
    32581 tagctgcttt taaggccact ttattttctt ctgaaacttt cttatctgtt gtaaataaag
    32641 tgccatctaa atctaaggca agtattttaa tatccgacat taatccacca tactttctaa
    32701 ataagccatt acagcttgat tattgtgatg acctataata caatctgcta tctctttaac
    32761 ttctggtcta gcattttctg ttgcgatagc ttttcctgac cactctagca tttctaaatc
    32821 attaatattg tcaccaaaag ataagacttc ttctgctcta ataccgtatt gttcacagag
    32881 gtgctctaaa ccatttcgtt tattaactga gcttagaata atatcaatgg atttaaaacc
    32941 tgttgtcact gctgtggtat acggtatagc ttgatttacc cactcctcag cttgtctaac
    33001 agtctcttca gtaaagttag ccgttacttt gaaaataata tcatcaacat cttcaaagtg
    33061 agaaactttt tgcaaattat cataataatg agtgatgaat tcaatataat ctggattggc
    33121 atcacttaga atatatgcac catccttgcc agataagaca tattcattat tctccatata
    33181 agggctctta ctcaaatggt caataatatc taagtattgt tctcgactca agtgctgttc
    33241 atatgccaag cgattaaata agactgctgc acttccgttt tctgctataa aagccatctg
    33301 atcacgaaag tcagcaaata attgttcaag tgataataag gaacgcccac ttgctgccgt
    33361 aaagacaatt ccttgttctt taaatttttt tagcacattt gctaaacgtt ttttatcata
    33421 ggtaccgttt tcatctaaga atgtaccgtc catatcggtt gctactaatt ttatcatgga
    33481 atctcctaac taaagggttt acatttacta ttgtaacata aaataaggct gaaaatccaa
    33541 aaaacagaag ggatcccctt ctgttttcta attggtcatt tcttgagctt cttttaattt
    33601 tacagaaaca attttggaaa cgcctgattc ttgcatcgtt accccataga tactatcagc
    33661 agcagacata gtcccttttc ggtgagtaac aacgataaat tgacttgatt tatcaaatcg
    33721 attaagataa tcgccaaatc gcttaacatt tgcttcatct aaagcggcct caacttcatc
    33781 caagatgaca aatggaattg tcttaacacg gataatggca aataataatg ctaacgctga
    33841 taatgctttt tcaccacctg acatcaagtt taaagactgg atttttttac caggaggttg
    33901 aaccgaaata tcaaccccag ctgataataa atcaccttct gtcagaataa ggtccgcaga
    33961 acctccacca aacatctgta caaaggtttc tttgaaacta tgacgaatgg cttcaaatgt
    34021 cgatttaaaa cgagtcttaa cttcatcatc catatctgta atcgtctcta gtaataaatt
    34081 tttggcatga actaagtcgt ctcgctgtgt gttaaggaag gtcaatcgtt catgaacttc
    34141 ttcaaattga gcaattgcat caatattaac aggtcctaaa gctttaattt tagcttgtag
    34201 agatttcaat tgttcacgcg ccatcaaaat atcttccaag acatttgctt ttaccttcgc
    34261 ttcatccaat gtcatctgat agtcttctga taattgacgt gagaatatca tcaattgttg
    34321 agataattgt tcacattgtg actctaattg agcttgctga cgaataagac tttcattttt
    34381 ttgtccctct ttagccagag aagcagctaa atcatctaaa gcagcttcac aatcctccaa
    34441 ttcaaaacgg agactcacaa gcttttcttc gtcattttct cttctattat ttacttgtag
    34501 caactgtttt tctatacgtg gaagctcttc tttgtctaat tgactgtcct gatggcttag
    34561 taatgtttgt aaattactaa tgtcacgttt tatttcagac aaggtaatct ctagacgtgt
    34621 acagttggca cgttcaaatt tctgctcgtt tagcaaatca cgttcttcta gctgtgcctc
    34681 tgacaataat gttgttagat tattgacttt ttcttgaatt gaatctttat cttccttaat
    34741 ttgagcaatt gaagttgtca actcttcttt tttaatagca aattgttcaa gttctttttc
    34801 taaacgattt ttttcatttt cagaagtagt ttgttccaac gccccgctgc ttaattgcaa
    34861 gccattatat agtttattta aatcagcaag cttttctgat agttgttgat actctatatc
    34921 agctctttgt tcctcaagac gagcttgttc tccatcattc ttaagttgtg ctaaggtttc
    34981 ttgtttctct ttgagttgct ctaacaatgt ggcaacttct ttttcttgaa ttaattgttt
    35041 actttgtgcc tgtttcaatt ctttttttag attatccaat tcaggcttga taaaaacagt
    35101 attattttga cgatttgctc cccctgagta cgaccctcca gggcgtaact ctgttccatc
    35161 taaggttact aaacgtactt gataattcaa ttgtctcgca gctacattag cattatcaac
    35221 cgtatcaaaa atagcagtta accctaagtt atttttaaaa atgtttgaca agcgttgatc
    35281 ataggttacc aattcactag ctattcctaa aaacccttga gaactttgta attttgagag
    35341 ataatgttgt gctaattctc ttggttttat tgtagttaaa ggcagaaatg tcgctctacc
    35401 ttggcggttt ttctttaaaa aagcaattga acgtttggca gcactctcgt cttcaacaat
    35461 gatgtgttgg ctgctacctc ccaaagctat ctccaatgct gtttggtaat gtttgtcaaa
    35521 gctaagatgc tcactaacag cacctatgat acctccaagc tggtccttag cctgtagaac
    35581 tgacttcact ccagcataga aattactatg atttttcaaa atagattcta aactcgaaat
    35641 acgtgcttgc tttgatttga tttcatctaa atgatcaaat aaaattgact gctgattctt
    35701 atactcacct tctagatttt gcaaggtttg acctaaatct tggtagtgag ataataactg
    35761 acgaacattc ttctttgcag cctcaaatcg ttccaatgct acctttgcgt tactttttaa
    35821 tacttctaaa tttttgctaa cttcttgtat ttcttgagat ttagcttgag aagcttgttt
    35881 ttgattctct atatcagcta gtaaagctgt taaagcgttt gaagtgtcgg cttcttcttg
    35941 catcaaggta acaaagtctt ctcgaagttt ttcaataatc tgatccggat tagttgaaaa
    36001 acgctccaag gaactttctg tactaacaat ttctgatctt atttgggcta ttttttccga
    36061 cagagtagtt aactctatat ttttttgggt aatctggtca ctacaatctt tagctttaat
    36121 ttctaactct gctaagcgtt gacctgcctc ttcttttttt tcagcctttt ggtttgactc
    36181 taaacgaatc agatcaattt ggcgttccaa atctgacttt aactttgtaa cgtctaaaag
    36241 agctaactgt ttagcctcaa tttcttcaga taaatggtga cgtttctgtt ttaacgattg
    36301 attttcatct tcaagggact gacgttgttg gtaataagtt gctaactctt ttcttactgt
    36361 caatagcttt tcctcaacag tagttaaatc tgattgatgt tgtaaaatat cttcaatcaa
    36421 aatagataag tggagtcctt gtcgttcctc atctaatact aaaaaacgct tggcaatact
    36481 tgcttgtttt tctaatggtt ggacttgcat gtccaattca taaataatat cctctaagcg
    36541 atctaagttc ccttgagttt gttctaattt ggattgtgtt tctttcttac gtgttttgta
    36601 tttcagaaca ccagcggctt cttcaaatat ggcacggcgc tcttctggtt tactattaaa
    36661 aatagcttca acgcgtcctt gggaaataat agaaaaagaa tctcttccta atccagtatc
    36721 cataaataaa tcatgaatat cacgtaagcg aactttgcgt ccatctatta aatattcact
    36781 atctccgttt cgaaaaatac gacgttcaac tcgaacttcg tcagctatat tttcgataaa
    36841 atggtcgcta ttatctaaag taacactcac ttgcgcataa ttaaggggct tacgattttc
    36901 tgtaccagca aatataacgt ctggcatctt acctcctcgt aaactttttg cgcttgattc
    36961 tcccaatgcc caacgtaaac tctctgtgat attactttta cctgatccat ttggacctac
    37021 aacagcagta actccttgat caaattctac cttagtttta tccgcaaagg acttaaagcc
    37081 ttgcatttca atttctttta aaaacatgat ttcctcattg taacgttgct aaagcatttt
    37141 tggcagcgtc ttgttcagca gctttctttg atcgtccaat tccttggctc aaattttctt
    37201 ggttgacaga tacagtcacc tcaaattcct ttgcatgtgc tggaccactt tcattagtaa
    37261 cttgataatc tatttttaca tccccatgag attgaagtaa ttcttgcaag cttgtcttgt
    37321 aatctttaac acgctcatag gtcccttttt caacgtgagg gatcattacc ttgttaacaa
    37381 aggcatgaac cacttcaaca cctttatcta ataataaagc tcctaaaaaa gcctcaaata
    37441 aatctcctaa aatagtgtca cgattacgtc caccagattt ttcttctcct tttccaagtt
    37501 taatatagtg atcaaagcca catagacgtg aaaaacctgc aagactttcc tcacgaacaa
    37561 tcattgaacg taattttgat aaatcacctt ctgccttttg agggtacttt gtaaaaagat
    37621 actgtgaaat aagtaattgt agaacagcgt ctcctaaaaa ttccaaacgt tcattatgtg
    37681 aaatgtttag gaggcgatgc tcatttgcgt atgatgtatg ggtaaaagct gtatctaata
    37741 attcttgatt agcaaaaaca ataccatagt ctttttccag ctttgatctt aattctttca
    37801 tttttttctc cttgatttca atcatagata aaatattaaa caaataccat tataccacag
    37861 ttgacaaata atc
//
LOCUS       DATRNK010000012        35896 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.012, whole genome shotgun sequence.
ACCESSION   DATRNK010000012 DATRNK010000000
VERSION     DATRNK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35896
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.012"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            249..425
                     /locus_tag="VBC52_000627"
     CDS             249..425
                     /locus_tag="VBC52_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882229.1"
                     /translation="MHVGKSYFDYQENALIPQLGFLYSKLTKKIELDNRLSPTEQKLL
                     ITLLLHTKGLIIDM"
     gene            1770..2039
                     /locus_tag="VBC52_000628"
     CDS             1770..2039
                     /locus_tag="VBC52_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882230.1"
                     /translation="MVSLLLLKVKQEELIEFHNVFYREAISSYLETENFEKAKVTEDN
                     YQVFLGIRTYQKSYNYQDHSENLKGNIEVAMIGVENNISDEHIFC"
     gene            2100..3251
                     /locus_tag="VBC52_000629"
     CDS             2100..3251
                     /locus_tag="VBC52_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6882231.1"
                     /translation="MVRKFLKSLLSLFLIAVIATGISVACFFFIPENKGNITPILLHR
                     FMRKNNVNGMMIVSDNTGKPITISHGINRGEVETDIENNKLFPMASLQKLMTGIIIQR
                     LIDQDVLSEDDRLSQFFPQVKGSNSITIHQLLTHTSGLREKGVKVSPYLKNEREQLQF
                     CLKHYNFVNKKSWYYSNINFSFLTGIATQVTGRTYAELVDDVIKNPLRLDDTQSYQSV
                     VNHDLVSPMRKNGKLNKINIFNQVSTAYGAGDFFTTPLNFWVLMRSFSKGYFFPTDEY
                     TKHQNDAISHYYGGLYMHGRIVNSNGAFFQYSSFGYADLKTHDTMVLFMNNKVYSDAS
                     HVAPKAFEYYQKFMFLNKIFHLVFYFVFSFFLMLLIRHSFRKRRYKKRL"
     gene            3451..4443
                     /locus_tag="VBC52_000630"
     CDS             3451..4443
                     /locus_tag="VBC52_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6882232.1"
                     /translation="MSMIEVSHLQKNFIKTVKAPGLKGAFQSFLRPEKHTFEAVKDLT
                     FDVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRIDGKLPQENRQNYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDKEFRKRMAFLNEVLELNDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCHRIFMIDRGQEIFDGTVSQLKETFGKMKTLSFDLRPGQEHISSSLIGKSEIN
                     IKRNDLVLDIQYDSSRYQTADIIQQTLADFSVRDLKMTDADIEDIIRRFYRNEL"
     gene            4446..5264
                     /locus_tag="VBC52_000631"
     CDS             4446..5264
                     /locus_tag="VBC52_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEO6882233.1"
                     /translation="MNRIWRRYKPFISTGIQGLITYRVDFILYRIGDVIGAFVAFYLW
                     KAVFDSSSQSLIQGFQLSDMILYIIMSFVTNLLTRTDSSFMIGDEVKDGSIIMRLLRP
                     VHFAASYLFMEIGSRWLIFLSIGVPFLLVITGVRLFLGTDLIQAIVLVVFYIISIILA
                     FLINFFFNICFGFSAFVFKNLWGSNLLKNSLVAFMSGSLIPLTFFPKIVADILGFLPF
                     SSLIYTPVMIIIGKYDGSQIVQALLLQIFWLIVMVALSQLIWKKVQSHITIQGG"
     gene            5266..6051
                     /locus_tag="VBC52_000632"
     CDS             5266..6051
                     /locus_tag="VBC52_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6882234.1"
                     /translation="MTKYQRMHFIFIKQYMKQIMEYKIDFFVGVLGVFLTQGLNLLFL
                     NVLFQHIPSLEGWTFQQIAFIYGFSLLPKGIDHLFFDNLWALGQRLIRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGFILLSTTVSSISWTVPKVLLFIFIIPFATLIYTS
                     LKIATSSIAFWTKQSGAVIYIFYMFNDFAKYPVAIYNNLLRWIISFVIPFAFTAYYPA
                     AYFLQDRNVYFNIGGVILISLISFMVSLILWHKGVEVYESAGS"
     gene            6676..6981
                     /locus_tag="VBC52_000633"
     CDS             6676..6981
                     /locus_tag="VBC52_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylX protein"
                     /protein_id="HEO6882235.1"
                     /translation="MGRSNILSRCEQCSDNQCGLYKTRLKDKLLLVVQLVGNIRYSEF
                     LTEGGVYHKGETLLEVITLGISYEYKSYGDIKVIKKYCADGSVVDYNMPILLMERIE"
     gene            6981..7829
                     /locus_tag="VBC52_000634"
     CDS             6981..7829
                     /locus_tag="VBC52_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylD"
                     /protein_id="HEO6882236.1"
                     /translation="MKTVIIYGGQGNVQSKHLCQLVKTPKGEILFSKLQATDLESYQL
                     MMDVINHKVSLDNVHASMLANFLYNDWLTNDEKIVSGIGFSAHSAGIFNVLYASGSTS
                     FENIIHFIKRRAQLIENCHVSEELWLLMVENIDMFSKDVLKEQSEKVKLAIITDDRAG
                     VIAMDQVNRSFFDDVMTNKGYFYKLKQLGVKAPYHTDFLNESKEQYKQLIESLDISQN
                     DDYDYIFHCDQLVDEILYQWTNLFNWRAIKETIIEKDAEIIDISPNRFISSQLQKMKK
                     RGRKQL"
     gene            7826..8548
                     /gene="fabG"
                     /locus_tag="VBC52_000635"
     CDS             7826..8548
                     /gene="fabG"
                     /locus_tag="VBC52_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEO6882237.1"
                     /translation="MKVAIVTGGTRGIGRAITKELYKEGYKVIAIYNSNDAKARALQE
                     ELPKLDVYKCNISDAKAVQKLVTKIFREYGGIDCLVNNAGIVRDGFFLMMSKEKWMDV
                     ININIMGLVNMSKAVLKIMKAKRIQGKVINISSTSGIAGQIGQANYSATKGAIISITK
                     TLAKEFASDGITINCVSPGFIETDMTNELQNKEELKEHLIPLKRFGQPEEVAWLVSFL
                     ASEKANYITGKNIVIDGGMIND"
     gene            8541..8846
                     /locus_tag="VBC52_000636"
     CDS             8541..8846
                     /locus_tag="VBC52_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO6882238.1"
                     /translation="MINWAEERKIIKTRQEVVDEIKGILIDSLMLDIDKDLIMNDQPL
                     FGRGLELDSIDALELSIGISTTFGVELNDDDISILSSVNRLADFVIENSEDIDDQAK"
     gene            8830..9306
                     /gene="fabZ"
                     /locus_tag="VBC52_000637"
     CDS             8830..9306
                     /gene="fabZ"
                     /locus_tag="VBC52_000637"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO6882239.1"
                     /translation="MTKLSSEEIIKMLPHRYPFLMVDRVQSYKKGIIECRKNITINEP
                     YFQGHFPQRPIVPGVLMIEMAAQSAALLYILDALDGSLPSLDELTAEKIAEKVGYLAS
                     VKNFKFKKIVTPGDQLNITCKSQSKLGHLLEIQVVIRDENKKEVASGRMLVSENGN"
     gene            9296..10225
                     /locus_tag="VBC52_000638"
     CDS             9296..10225
                     /locus_tag="VBC52_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6882240.1"
                     /translation="MEIKLKNIGKKYGSFEALRDINLIFEEGKFYGLLGPNGAGKTTL
                     FNLLIQNFKQTSGDIKWEVDGKPLSIKDFYRHIGIVFQSNRLDDNLTVEENLISRGAL
                     YGLSKSQVRNRLKDLQTYLDITAIKKQKYGSLSGGQKRKVDIARALLPQPSLLLLDEP
                     TTGLDPQSRRDLWDAIAQLNQQSQMTVVLITHYLEEMSACDVLNVLIEGNIYYSGDIK
                     SFIEQHSTTNLNVVLKPEKSLDQLSIADFVNKCQVLSEREIVFKDISVEEMMQVISDN
                     QDKSIIETFNVEYSNLEAAYLNLLREKERKNNV"
     gene            10218..11096
                     /locus_tag="VBC52_000639"
     CDS             10218..11096
                     /locus_tag="VBC52_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6882241.1"
                     /translation="MFELISRNRKVYTRDRLAFFMSFLSVIILILVYQIFLGKIQLDA
                     IKAALRSNTVSSDTIHMVNYWLIAGLTTIISMTSTLGAFGVMVSDKEKKLNEDFKVSP
                     ISNIKIELSYAIFAVLFGIVMTMFSCIFAIGIFNGFNSLLEYSAMDYLTILGIVSLGT
                     VLSAAMILPILAFIHTSSAFTTLSTIVGTFIGFISGVYLSIGSVGKALQQVMTWFPLT
                     QINSLLKQVLMKGSIAKVFDKANEATVSNYKESYGVVLRNADGERLSNHFMLIYIIAL
                     ILILLAIHFIIKKVKK"
     gene            11093..13096
                     /locus_tag="VBC52_000640"
     CDS             11093..13096
                     /locus_tag="VBC52_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylE protein"
                     /protein_id="HEO6882242.1"
                     /translation="MKDDNKLKISEASLEDYSEVVHLFNRNHVYQFPDGSPLTVDDLD
                     LTLKVKEVTHLFLLKNHGVLIGTSAFFKFITYGCLDWNSSFSGFLLIDSKSRSGQAIT
                     YLYKTILKKITKLKFSNIYTEISNYNKPSLALSKLNGFKEYDKTYEDILHCRSLRSHL
                     PKILNTFRISNYYGKTYDISTFQIMEEIENPLEEETEIRTKVSDEEILFKAEDSASLP
                     YYLKMSLFQMEIARLDNRYVLQVDFLSEQVKRVRVKTGKYHLANLTRAHPSLTLSRFA
                     NYYYIQATVETLYGNIDVQLERRKKHYRDATICLKRTFQGYDLLISPNGSLIFEKQKR
                     KILEDSFLIFSQPLDKKLVVKEEENHITIKCFYQGALIEKIMTFTSDEEITCVYKCNQ
                     KAKEMFPKLLKQTFKLHCQEQLIRDSEGYLVNVPGSYPIEHDDFLRADKFEDRQFHYY
                     LPNEDKMISYSPPGKASNQMQFRPLCLIDTDSLSFPLTYHFRISQASSEEALINLKKQ
                     PIWDSNYQASATDLLKHISNLTLEEEKDYGIKRMIANRKHYPSHKLVLAYNQIVLPKN
                     EIPRDSELYSISFDYRIRGKFVQIRQGEHVKYDNKSYVLENSQQLVLYVASDDKYILI
                     SAKNGIFYSYKENNHLKIRCMFKRNSPYATNVSITEYRKCEEK"
     gene            13093..14046
                     /locus_tag="VBC52_000641"
     CDS             13093..14046
                     /locus_tag="VBC52_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEO6882243.1"
                     /translation="MNTIYNTLRTDKGYKVYEGYLYEITGEECEEALDLVIPKNIVFA
                     DTDTCGYTFLLNEDGTVYDDVTFYKFDDKYWLASHKALDSYLDNINFDYTVTDISDEY
                     KMLQIEGRYSGEIAQSFYEYDISTLNFRTLIEMTYKGEKGYLARFGFSGEFGYQFFLP
                     SSIFATFVSDVCEGIAEYGDELDRYLRFEVGQPITDIYQQEEYSLYEIGYSWNLDFTK
                     EEFRGRDSLLEHIRSATVKSVGFSTKEKLASGTPVLFDDQIVGKIFWIADEKDSSENY
                     LGLMIVNQTYAHSGVTFVTEDGQILKTQSSPYCIPESWNKE"
     gene            14043..16238
                     /locus_tag="VBC52_000642"
     CDS             14043..16238
                     /locus_tag="VBC52_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEO6882244.1"
                     /translation="MSVYVSGIGIISSLGKNYSEHKQHLFDLKEGISKHLYKNHDSIL
                     ESYTGSITSDPEVPEQYKDETRNFKFAFTAFEEALASSGVNLKAYHNIAVCLGTSLGG
                     KSAGQNALYQFEEGERQVDASLLEKASVYHIADELMAYHDIVGASYVISTACSASNNA
                     VILGTQLLQDGDCDLAICGGCDELSDISLAGFTSLGAINTEMACQPYSSGKGINLGEG
                     AGFVVLVKDQSLAKYGKIIGGLITSDGYHITAPKPTGEGAAQIAKQLVTQAGIDYSEI
                     DYINGHGTGTQANDKMEKNMYGKFFPTTTLISSTKGQTGHTLGAAGIIELINCLAAIE
                     EQTVPATKNEIGIEGFPENFVYHQKREYPIRNALNFSFAFGGNNSGVLLSSLDSPLET
                     LPARENLKMAILSSVASISKNESLSITYEKVASNFNDFEALRFKGARPPKTVNPAQFR
                     KMDDFSKMVAVTTAQALIESNINLKKQDTSKVGIVFTTLSGPVEVVEGIEKQITTEGY
                     AHVSASRFPFTVMNAAAGMLSIIFKITGPLSVISTNSGALDGIQYAKEMMRNDNLDYV
                     ILVSANQWTDMSFMWWQQLNYDSQMFVGSDYCSAQVLSRQALDNSPIILGSKQLKYSH
                     KTFTDVMTIFDAALQNLLSDLGLTIKDIKGFVWNERKKAVSSDYDFLANLSEYYNMPN
                     LASGQFGFSSNGAGEELDYTVNESIEKGYYLVLSYSIFGGISFAIIEKR"
     gene            16243..17454
                     /locus_tag="VBC52_000643"
     CDS             16243..17454
                     /locus_tag="VBC52_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylJ protein"
                     /protein_id="HEO6882245.1"
                     /translation="MSKFLFAPFMMNLGESQRLSKLANCLYEQGHDIHILGDNYYPFL
                     FNNDAYRYHHCVEDKSVYNSKRYDAFFSLSTDFNFLSEEEIERICSIERDLLRQEKFD
                     AVLTGYRLSIVTSCRLESIPLIWIISGATHISEIVENSEGILPNGKISKASKPQTKDF
                     IKRVITTYSTNVKTWNNYIKKYGGKPFNNALELFTGDLNLVTDYSLFYEFDKDSSYKT
                     IGPILIDNVGFSKCSQINQDNKTVLLSFGTSFKRDWVESFLKTLPRHYHYLLTSCGEA
                     INIPGSYIEIVDFIDFKTIDKEIYFAIIHGGQGTVYAMAAQGIPFIGIPFFNEQFWNI
                     KKFSKEKCAYLVKKPDVREIRKAISCLERDYDIYKSYMIRLSQGIKEEAEASLQKAEA
                     EIESFIRKRQS"
     gene            17423..17998
                     /locus_tag="VBC52_000644"
     CDS             17423..17998
                     /locus_tag="VBC52_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylK protein"
                     /protein_id="HEO6882246.1"
                     /translation="MNPLSEKDKVNYIMIYKNRQSTLENNLSGDRLMRYQQANRQVSK
                     DKLLTGSYFVYQCLKKLGFTNKICQESFSSVSSYLIGLPKGKISYSNSGDYHILTYAP
                     SGSTGVDIEKYKDRSEQTYQNYLGESVSSDMDSKLLFYKAWLQKEISYKCGKSIDITY
                     QTMIDGYIYGYAFDNTFKVEAVYLKELLPDN"
     gene            18341..18682
                     /locus_tag="VBC52_000645"
     CDS             18341..18682
                     /locus_tag="VBC52_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882247.1"
                     /translation="MSEFLSAKPIGHNDSSNTILRIIENTKYIHHYDGKVVVEIPPQK
                     EPIKVFFMDSRLHYLEFSDICRYVTVVLKNSTVDNITNLGLDNHIVYDNGDDMVDPLP
                     SIQHGLFLNDD"
     gene            18716..19231
                     /locus_tag="VBC52_000646"
     CDS             18716..19231
                     /locus_tag="VBC52_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882248.1"
                     /translation="MTFYKKTDKFGFRKSKVCRSLCGALLGTVAVVSLATASTEIHAD
                     EATTSPTTVTKVPQPVQADTTALNTSKTHSTQATTTPVEAKENKVVKSETVQSESRVM
                     PRDKVVERPETVKASVNSDVSQPITTTPPTINEKTVEIPNLAQDTKKVAPKVTVTPEV
                     VNQPKQLPKYE"
     gene            19283..21940
                     /locus_tag="VBC52_000647"
     CDS             19283..21940
                     /locus_tag="VBC52_000647"
                     /inference="COORDINATES: protein
                     motif:HMM:NF017709.1,HMM:NF018035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO6882249.1"
                     /translation="MTKPQGAVAEKATPAVPKPQKVRVIVEFNKESILDYATEQKKTV
                     AQLNQADVEKKLQSIKQEQDKVLKNIEKSVHFDSSKVKRYDAIINGVALDIQAQEIEK
                     LKTIADVRRVYVSQEYVQTKPLLSSSGQLIGLPEVWNNSQYKGEGTVVAVIDSGVDFK
                     HQALKIKEPNRAKYNKTSIEKLIHEKNLKGKFYSEKVPYGYNYYDYNDNLKDSYGVMH
                     GMHVTGIVGANDDNQKLYGVAPNAQILAMKVFSDDQQNPTTFTDVWLKALDDAILLKA
                     DVVNMSLGTPAGFVHEGKDYPELEVIARACKAGIVIAVAAGNEGNITDGNTYGVKPLA
                     ENYDTALIANPALDDNTLAVASMENLKKHAHVLKFKDKKSGTEVTEVINLHVAPNASK
                     TIIGLAVDLGAGAPSELSKHFDLSGKIAMLEIPEDNKSNGFLEKVQAITKLNPAAILL
                     YNNAKVKDDLGSQLLVESEAAKFNIARITRSTYNNIKNNSNKIITILTERQAIDNSLA
                     GQLSSYSSWGPTPDLRLKPEITAPGGHIFSTVEDNQYADKSGTSMAAPQVAGAAAVLK
                     QYITDKKIPVDNAADFIKLLLMNTAQPIINKQSKDGKTPYFVRQQGSGAMNLAKALVT
                     TVVATVTGTNDNNADGKLELRELKEKKFKARILLRNFGKTNKTYIISSEAIADPVDEK
                     GFRTQNSEHLVSKKADAVTRKVTVEAGKTLAVDLDVDYSDAEALTRNNFLEGYLNLKD
                     TEGVADLHLPFLGFYGSWTEQKAIDAFEGISEIGNGDKKRRVQFYVNKETNKTSSTFT
                     TNGMLSLPIYNNTVFFSPNSPFYDKAGVRIAALRNMEYVQYSIIDPDTNKEVRVLGRS
                     HDVRKLYRLDYRNSFAMMPDSIWDGKIKD"
     gene            21986..25303
                     /locus_tag="VBC52_000648"
     CDS             21986..25303
                     /locus_tag="VBC52_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882250.1"
                     /translation="MNNKGVGGDGVQIYQYYIKMDNNKPYLSPKDKTTVEKLEDRWKK
                     ITFKVQDTGIGLKDVYLQSVKYVGGGNNNLDLITPPGFKKEDKKVEKPKLDRPPGIDL
                     PAPTSMRSFDYSTPPGTKPSKPKDSLSTPPGFPDLNTPPDEAPKDSKKDAIEDKSGAI
                     KYAKSLQLSFVDDPILASKVNGKILQVESDGKLVIPRNALSANQFDDTSLKIYRNNNR
                     NKEITITTDYFADTKYVNITAVDYLSNTTFEQLATGETVDYHAIVFSSFAAIKDKGGK
                     IYVNDKLQETSRIALKDKSVKIGIELPNDVRHIDSLSVRRLNEVKTVDNILKNDEQDI
                     NLSKTYQLKYNPTNRRLEFTINNINSSSEIMTTFKDGKMPELVEQKDVSLDINDMDMS
                     KFKTIRLGRKDSEFKGQLIAKTGTVELDMFFKQSQDPASIIKKIYLIQNGVPNELKKF
                     DSSFGLTESQIDGYYIYKDAINLKFKLTSGASLKVVYKGQEDPYSHQKEDMTKKGEQL
                     SHSTQANENTAKVTFANIDWSHYSKVTVNGKEVGKGSELPLTKGWTTFVLHKTENSLN
                     VKSLIMETGSVSKKVQQLPLSPRLSKNKHMRDMLLTMQKDSAYYETSDSLVLRINLTA
                     DTKLNFNAVKGASALTENMMMRQFAVAGPQDDPVSEHKYPSVFLLTPALLETASEATL
                     NGKEITASGIIGHIKDGDKSKHVEVKMVNENGDMLGTPVIIQGKDLTNRTKPLMSGRR
                     VLYAGKQYEFRAKLPLSRFNTWIRVEVVTEAGEKASIVRRMFFDQSVPELNTAVAKRD
                     LTSDTALIHIVAKDDSLKLKLYQDDSLLESVDKTGLYSFRNGVEITKDMTVPLEFGDN
                     IIKLSAVDLSNYRRNETLHIYRNRFDVKASQMTADKGAKVTVDMLMKHLVVPEMAGAY
                     TLTIDEAPNTNESGMLTNAKVSIHYVNGGVDKVDVPIKVVDLEAIRKAEEAHKADEAR
                     KAEEARKAEEAHKAEEVRKAEEAHKVEEARKAEEGHKTQEAPIVEEGYKVNNVHQTDT
                     TVKASDLPKTKTVSAVHMARTDNKQITSHQTHVEKQIKNTLPSTGDSKRGYYITGMAI
                     VMLSVLFSLAKKFKSKY"
     gene            25487..26572
                     /locus_tag="VBC52_000649"
     CDS             25487..26572
                     /locus_tag="VBC52_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M13"
                     /protein_id="HEO6882251.1"
                     /translation="MQRKSYLKSMSVLTLTACLISGYVVKDIAMLHAVSASEKKANNV
                     SPRENLYRAVNDNWLANTKLKQGQISVNSFSEIEDKLKQLLVSDMAKMASGKIETTND
                     EQKKMVAYYKQGMDFKTRDKNGLKPLKPVLQKLEAVSSMKDFQSLAHDFVMSGFVLPF
                     GLTVETNARDNSQKQLVLRQAPALLESPDQYKKGNKEGEAKLSAYRTSAMALLKQAGK
                     SNIEDRKLVKQAIAFDRLLSEKTQVDQSKITAESETAAGRYNPESMETVHNYAKEFDF
                     KELIEKLVGPTNKAVNVEDKTYFKQVNDVINSKQLANMKAWMMISMLVDQSDFLGEQN
                     RQAASAFKNVASGLTQIESKEKMLTPN"
     gene            26593..27411
                     /locus_tag="VBC52_000650"
     CDS             26593..27411
                     /locus_tag="VBC52_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEO6882252.1"
                     /translation="MGDYYGKKYFGEAAKKDVEHMAKKIINVYKTRLKNNTWLSENTK
                     AMAIKKLDNMRLMIGYPEDYPDLYRQYQFDSKASFFENNDNYRKLSNKKTFEEFNQSN
                     QREHWQMSANAVNAYNDPNTNSIVFPAAIFQSPLYDKTKTVSQNYGAIGAIIGHEISH
                     SFDINGMKYDEKGNLHDWWTKEDLKHYKKKTQAMIDQWDGLKADGGKVDGKLTLAENI
                     ADNGGVMASLEALKTEKIQTIKNFLNHGQVFGVKKQPKNKVSPQFSQMFMHHMN"
     gene            27550..28581
                     /locus_tag="VBC52_000651"
     CDS             27550..28581
                     /locus_tag="VBC52_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6882253.1"
                     /translation="MKNITKLSTVALSLLLCTACAASNTSTSKTQSHHPKQTKLTDKQ
                     KEEPKNKEAADQEMHPQGAVDLTKYKAKPVKDHGKKIDVGDGKKMNIYETGQGKIPIV
                     FIPGQAEISPRYAYKNLIERLSKKYKIYTVEPLGYGLSDIPTKPRTLENITKEIHTGL
                     NKIGVKNFYLAAHSLGGMYSLNYAKNYPEEVRGFIGMDTSTPWMEGEQKTKYDPESAK
                     WAMKLPDVDDKTNEQYLSIAKKINGNKSQRDEDERLGDNLGKLKNVKFPKGMPTKYFL
                     ADQSVEGIKLRQADFKGLKNWDQQHIDLSEDPKDASVEILKGSHNIFQTQYEHMAKAI
                     DGFIQSHQK"
     gene            28616..29635
                     /locus_tag="VBC52_000652"
     CDS             28616..29635
                     /locus_tag="VBC52_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO6882254.1"
                     /translation="MNKITKLSTVALALMLCVGCSANKDNQKTKTEQPKQEKSIAKQK
                     QEREAEKAMIAKGAIDLTKYKAKPIEDYGQKIDVGDGKKMNIYTMGQGKIPLVVFPGQ
                     AEISPRYAYKNLLDRLGKKYKIYTVEPLGYGLSDMTDKPRTIENITKEIHTALEKVGV
                     KNFYLMAHSLGGIYGLNYAKTYSNDVKGFIGMDTSTPNMEGDGTTGESSASTLKWAES
                     IPEVDKKTNEQYLSIGAKKSGNKTERDEDRRVSDNLHKMEKVKFPKGMPAKYFLANES
                     AEDIKMRKKIFPKLKSWEQQHIDLSEDPKDVSVETLDASHLIYHTQYKHMAKAIDEWI
                     QSHKK"
     gene            29670..30731
                     /locus_tag="VBC52_000653"
     CDS             29670..30731
                     /locus_tag="VBC52_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6882255.1"
                     /translation="MNKITTLSTIALTLMLCVGCSTNKDNQKTKTEDPKQENVTANSK
                     EDKATAAKRMKLTKELEKYNKGTESFISNKQYQKYIKTPIKEYGQKVEVKGKKMNVYT
                     VGEGKVPIVFIPGQGTVTAKHQYHNLISNLSKTHKVVVVEPFGSGLSDVIDQPRNLAN
                     ITSDIHEALQKVGITGKYVIASHSIGGVYALKYISTYPKEVLGLIGLDTSTPGMEGGK
                     QVDFAAPVLKELPKIPKVSDDINAQFFAIGHKILNNSNMKEEAKNSSNMINESANYKI
                     PKGIPAMYLLARESDLGLQLRSLDIPIKGSWQEQHKELSEDPKEVSIHTLDGTHQIYD
                     TNLKEVIDYTNQFMKTFEK"
     gene            30855..32084
                     /locus_tag="VBC52_000654"
     CDS             30855..32084
                     /locus_tag="VBC52_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6882256.1"
                     /translation="MNLWTLSLAYLKRQKMKTVTLFLVFLTIGTCLISLMSIQHSLEK
                     NILTKQGKSIYLTSKEKAYWPEQAYEALKKAKMVESVEASLSIDVGSRLKSVSPYNSS
                     KEENQVTLAGYQSTEDLRAFQTKALVLKKGSHLAADNTKQVLVSLKLAQKNHLSVGNK
                     LRLGKENVTIAGIYDANSAKSKNTFNPNIDNTLIAQATLVRKISKQKGYQTVAVRLSD
                     KRLVDTVIQNIKQWPLDFGKLDVQTAKEFYGDSYRNIETLHRLVGRIILIVSLVAMAI
                     LVVMLTFWINNRIKETGILLAIGKTKFEIIGHYLIEVLLVAGAAFTLSIIGGVFLGKT
                     FAAGLLSQVNGGVSSQIVQNSSLIIDRIDNLAVSVGVMDVFRLYAQGALICLFAVVLS
                     SYSILKLQPKQILSRMS"
     gene            32094..33435
                     /locus_tag="VBC52_000655"
                     /pseudo
     CDS             32094..33435
                     /locus_tag="VBC52_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            33532..34203
                     /locus_tag="VBC52_000656"
     CDS             33532..34203
                     /locus_tag="VBC52_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6882257.1"
                     /translation="MFELKNIAYRYKGNDNKTLENINYSFQSGVFYTILGNSDSGKTT
                     LLSLMAGLDSPTEGQVLFNKKDIKEAGYAQHRKKNIALVFQNYNLLDYLTPLENVQLV
                     KPTADKQLLLDLGLKEDMLTRNILRLSGGQQQRVAIARALVAGTPAILLDEPTGNLDF
                     DIARDITMRLKDFAHKEKRCVIMVTHSREIAHMADTALQLTGDNLKELSKESIAQSIL
                     QQRNL"
     gene            complement(34260..35705)
                     /locus_tag="VBC52_000657"
     CDS             complement(34260..35705)
                     /locus_tag="VBC52_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEO6882258.1"
                     /translation="MKKTIGIIGMGVSGLAVLLALSRKKEKELKQLAIFCFDDTVHFG
                     RGIPFQEDVDSALINSPINDISFDYRHMSDFMDWLEENYYDTNLTYVSRTLYGQYLSE
                     RTKLLLDKLPVTVIKTRVDKLNYLSDKQQWQVYIQDKLFPVTFDELHLACGALPVIDP
                     YHLEGKTDYIKDPYPIKHLAQENWKGKTVAIIGTGLAAIDVIKWLLLNTQSSVKAFSR
                     TNYFPSVRIVNDLPLTWHFFTNDNLKAFLSHPSKTFTIADFNDLMSGELEHLGFKNWK
                     NTTETFLSPGIDGLKRAFKYPKQLYLLQQLASRITVWLTDLWPLMSLSDKKEYKALYG
                     KAIINLRNPMPDESAKVILKAAQKGTFSLINNVKTIAKNDEGFSIEKEKQNHISVDRV
                     INATGYQLNTKNWTKANPLLKSLINQEFCQIDDQGGITILTENAQVLSPKYGPMPGLY
                     AHGALVNGVVYQNNSTIKIQQMAERAFSDNK"
BASE COUNT        12526 a         5386 c         6769 g        11215 t
ORIGIN      
        1 gagtatccta agtgctttcc attttgaaat tcaaatatag ctaatagttc tagaacttct
       61 aattgttttt cgtcgacgat atgaattttc aatcttaact gttaggattc cacctccctt
      121 tggttaaaga aaaaaggtca ggtcgtttag ataactttgt caaacaagct caagctatct
      181 aaaaatagtt tgaaatgggc attactctag tttttaataa gctatctgat gagcagaagg
      241 agaagttaat gcatgttggg aagtcttatt ttgactatca agaaaatgct cttatcccac
      301 aattaggttt tctatattct aaattaacta aaaaaattga acttgataat cggttgtctc
      361 cgactgaaca aaagttattg attaccttat tattacatac taaaggttta atcattgata
      421 tgtaagaagt aagtcagcta accgatcttt ctattctaaa acttatattg ttgctttaga
      481 aattttaaag agcgtggatg gcttcataat aaacagaaat cttaccaatt tgcgaagcca
      541 aaaaatatga tatttgaaga gtccaaagat ctaatagata gtccagttag agaagcgttg
      601 attataagtg ataaggattt tcaaaaatta aaacaagagc tattatttta accgacttat
      661 tttaaagact tatcatatct aggcttgctt gatgattcgg aaaaatacgg agactatact
      721 atttcaagga aaagatacaa aagtttcgaa tcaagtcttc aactatacat ccttcaaagt
      781 catcggctag agatttggaa ttatgaacca atccctttga ttactagaaa aataaatagc
      841 ttggagaggc taacgtgaca ctggttgatc caatctcgct ttatttaaca ctaagaatga
      901 tgaagaccct cgtattgaag aagaagttga gcagctagaa gataagattt tgagaatata
      961 atcgtttttt cgtgctacac taacttgtca gggttgcaac agacaaaaaa ataatttagg
     1021 agggattcgt tatgcgtaca aagtatatag gatatgaaat tgttttaagg ggtggttatt
     1081 aatatggata agaaaaaggt tgtttcaact atcgtatcta cagggtttct tatagtttca
     1141 atatcaaatt tttttagaga gctatcacct atactttttc tcttgaatat tattggattt
     1201 ttctgcttcc tattattatt ttctgccaac ataaataaat tgttcttaaa acgatagccg
     1261 ttatatagga tatggtatgt atgcatttgg tgaaattata ggtaaaacga atggaatagt
     1321 acttactcct caaagctatc gtgagagata taaaaggata tcgaggagtc actagatagt
     1381 tggatctcat tgaaaaaatt tgtttaaagt atctcatata agcataaaac gaggaatcaa
     1441 tcaaaatttc ttgattgatt ccttgttttt tggctctttg tcaactgtag tgggttgatg
     1501 aaaaattata ttctggagaa aaccaaattg gttttctcct ttttgatatt caaagtgata
     1561 tgaattctct ttttaaaata ttcaaagttt ctaaagctaa atgtttgtgc ttgatgattt
     1621 tgatgagatt attggttgct caagtttggt gtttgaataa ggcgattcaa ggacattgat
     1681 aaccttatct ctgtctttaa aaaatgtcct aaaaacagtt taaaagattg gagtgacact
     1741 agagaaagtg ttttaaacag ggtaaagaaa tggtgagctt gcttctcttg aaagtaaagc
     1801 aggaggagtt gatagagttt cataatgttt tttatagaga agcgatatct tcttatcttg
     1861 aaacagaaaa ttttgagaag gctaaagtga ctgaagataa ttaccaagtt tttttaggaa
     1921 tccgtactta tcagaagagt tataactatc aagatcatag tgagaatcta aaaggaaata
     1981 ttgaggtagc aatgatagga gttgaaaata atattagcga cgaacatatt ttttgctaga
     2041 atatataaaa tgtctttgaa ataatttagc cctgtgttat gatattaagg aggttattta
     2101 tggtacgtaa atttttaaaa tcgctcctat ctctctttct tatagcagta atcgccaccg
     2161 gtatttcagt ggcatgtttc ttttttattc cagaaaataa agggaatatt acaccgattt
     2221 tattgcatcg ctttatgcga aaaaataatg ttaatggaat gatgattgtt agcgataata
     2281 ctggtaaacc gattaccatc tcgcatggga ttaaccgtgg ggaagttgaa acggatattg
     2341 aaaataataa attatttcca atggcttctc tccaaaaact catgacagga attattatcc
     2401 aaagattaat tgatcaagat gtcttgagtg aagacgaccg attgtctcag ttctttcccc
     2461 aagtaaaagg tagtaatagt attaccatcc accaattgtt gacccatacg agtgggttaa
     2521 gagaaaaagg tgttaaggtg tctccttatc taaaaaatga gagagaacaa ttacaatttt
     2581 gcttaaaaca ctataacttt gttaataaga aatcgtggta ttattctaat atcaacttta
     2641 gtttcctaac aggtatagcc actcaagtta cgggaaggac ctacgctgaa cttgttgatg
     2701 atgtgataaa aaatcctttg cgattagatg atacccaaag ttaccaatca gttgttaatc
     2761 atgacttagt tagcccaatg cgaaagaacg gcaagttaaa taaaattaat atctttaatc
     2821 aagtatccac agcatatgga gctggagatt ttttcacaac tccgctgaac ttttgggtgt
     2881 tgatgcggtc tttttctaaa gggtactttt tcccaacgga tgaatatacc aaacatcaaa
     2941 atgatgcgat atcacattat tatggtgggc tttacatgca tggtcgtata gttaattcta
     3001 atggggcctt tttccagtat tcaagttttg gttatgcaga tctaaagacg catgatacaa
     3061 tggtgctttt tatgaataac aaagtctata gtgatgctag tcacgtggca ccaaaagcat
     3121 ttgaatatta tcaaaaattt atgttcctta ataagatttt ccacttagtc ttttactttg
     3181 tattttcttt ctttttaatg cttttaatac gtcattcgtt taggaaaaga aggtataaga
     3241 aacgtctcta aatcacatga ataaaaaaga aggctaagtt ggccttcttt tttattcaat
     3301 attaagcagt aacaccatct aagaaaacgt ttgcattgaa tttttaattg tgctataata
     3361 aatagctgtt aatcattatt atagtttaat aatctaactg tcgagctgat ttaagctata
     3421 aaaatactgt tgagtaaaag aggtattaat atgtcaatga ttgaagtttc tcaccttcag
     3481 aaaaatttta taaaaaccgt taaagcacca gggttaaaag gagcatttca atcatttctt
     3541 cgtcctgaaa aacacacctt tgaagctgtt aaagatttaa catttgatgt gcctaaagga
     3601 caaattttgg gatttattgg agcgaacggt gcaggaaaat ctacaacaat taaaatgtta
     3661 actgggattt taaaaccaac ttcgggcttt tgccgtattg atggcaaatt gccacaagaa
     3721 aaccgacaaa attatgttaa ggatattgga gtcgtttttg ggcaacgtac gcagttgtgg
     3781 tgggatttag ctctacaaga aacctataca gtgttaaaag aaatctatga cgttcctgat
     3841 aaagaatttc gcaaacgcat ggctttttta aatgaggttt tggaactaaa tgactttata
     3901 aaggatcctg ttagaacttt gtctctcgga cagcgtatga gagcggatat cgcagcttct
     3961 ttattacata acccaaaggt tcttttctta gatgaaccca ccattggctt ggacgtttca
     4021 gtgaaagata atatccgacg tgcaatcaca caaattaatc aagaagaaga gacaacgatt
     4081 ttactaacga cacatgacct cagtgatatt gaacaacttt gtcatcgtat ttttatgatt
     4141 gatagaggac aagaaatatt tgatggcaca gtttctcagc taaaagagac atttggaaag
     4201 atgaaaactc tttcttttga tttgcgccca ggacaagagc atatcagctc tagtttaatt
     4261 ggaaagtcgg agattaatat taaacgtaat gacttagttt tagatattca gtatgatagt
     4321 tctcgctacc aaactgctga cataattcag cagacattag cagatttttc tgtacgtgac
     4381 cttaaaatga cagatgcgga tattgaagat atcattcgtc gcttctatag aaatgagcta
     4441 taaggatgaa cagaatttgg agacgttata aaccttttat tagtacagga atccaaggat
     4501 taatcacata tcgtgttgat ttcatccttt ataggatagg tgatgtgata ggagcatttg
     4561 ttgcatttta tctatggaaa gcagttttcg attcctcaag tcaatcctta atccaaggtt
     4621 ttcagctgtc agatatgatt ctctatatta ttatgagttt tgtgactaat ttattaacaa
     4681 gaactgatag ttcatttatg ataggtgacg aagtaaaaga cggttcaatt attatgcgac
     4741 tcttacgtcc agttcacttt gctgcttcct accttttcat ggagattggt tctagatggc
     4801 tcatcttttt aagtataggg gtaccgtttt tgcttgttat tacgggggta cgcctcttct
     4861 tggggactga tttgattcaa gctatagtgt tagtggtatt ttatattatt agcattattt
     4921 tagctttttt gattaacttc ttttttaata tttgttttgg cttctcggca tttgttttta
     4981 aaaatttatg ggggtcaaac ctgttaaaaa attcgttagt cgcatttatg tcggggagct
     5041 tgattcctct tacttttttt ccgaaaatcg ttgctgatat tttaggattt ttaccatttt
     5101 catctttaat ttacacacca gtaatgatta ttattggtaa atatgatggc agtcaaattg
     5161 tgcaagcctt acttttacaa atattttggc taatagtaat ggtagcttta tctcaattaa
     5221 tatggaaaaa agtacagtcg catatcacta tccaaggagg ttgagatgac aaaatatcag
     5281 cgaatgcact ttatttttat taaacaatac atgaagcaaa tcatggagta taaaatagac
     5341 ttttttgtag gcgttttagg agtattttta acacaagggc taaatctttt atttctgaat
     5401 gttctttttc aacatatccc ttctttagaa ggatggactt ttcagcaaat agcttttatt
     5461 tatggttttt ctctcctccc aaaaggaatt gatcaccttt tctttgataa cttgtgggct
     5521 cttggtcaaa ggcttattcg aaaaggagaa tttgataagt atttaaccag gccgatcagt
     5581 cctttatttc atgtcctagt tgaaaccttt caagtagatg cattggggga gttattggtt
     5641 ggttttattc tgttatctac cacagtatct agtataagct ggaccgtgcc aaaagtatta
     5701 ttatttatat ttattatccc ctttgcaact ttgatttata cctccttgaa gattgctacg
     5761 tcaagtatcg ctttttggac caaacaatct ggtgctgtta tctatatttt ttatatgttt
     5821 aatgactttg ctaagtatcc tgtcgcaata tataataatc tattgcgttg gatcattagt
     5881 tttgtcattc catttgcttt tacagcttat tatcccgctg cttacttctt acaagataga
     5941 aatgtctatt ttaatattgg tggggttatc ttgatttcct tgatttcttt tatggtatca
     6001 ctaatacttt ggcataaagg agtagaagta tatgagagtg cgggttcttg attagtaagg
     6061 caagccatta atttttttga gtgtttagaa gttttaggct tactaactta gctttgttag
     6121 tgatgatata ctccccttat agtgtctagt gaatttttgt ttttttactg tccactataa
     6181 ggggagtata tcaggtgaag agggactatt agtgttgaga ataatgtccc tcatagaaca
     6241 ataattacca tatctttacc aaaataaagt taaaaacgct cttagatgtg ctttctaaga
     6301 gcgttttttg gtctataaaa ggtaagcaaa aagcagatgt catttatgaa agtgttgaca
     6361 aagatgaaat actttgttac tattttaaca taataatgat attttaatta gagtgtgaat
     6421 ttataattaa tttttataat gtttatttta aatttaaact aatgtttata gcagtgtttt
     6481 actcatgatt ttgtgaattc aacacttaag tcatgacctc attttaaaga gtttcagacg
     6541 acacagaaag gaagaaaaca taagaaaggc aatgcacggc ttgtccatag tagtgtttga
     6601 agtagatgtt tagaaatatt ttattttcca acagtactga tatcagagcg aagatgatta
     6661 gttgaaggtg ataatatggg acgttctaat attttgagta ggtgtgaaca atgttctgat
     6721 aatcaatgtg gcttgtataa aacgcggctt aaagacaagt tactattagt agtccaactg
     6781 gttggaaata ttaggtattc tgagtttctt acggaaggtg gtgtatatca taagggagaa
     6841 accttgttgg aagttataac cttaggaatt tcttatgaat ataaaagtta tggggatatc
     6901 aaagtaatca agaagtactg tgctgatggc agtgtcgttg attataacat gccaatacta
     6961 ttaatggaaa ggattgaata atgaaaacag taataatata tggaggacag ggtaatgttc
     7021 agtcaaaaca cctttgtcaa ctagttaaaa ctccaaaagg tgaaattctt ttttcaaagt
     7081 tacaagcaac tgatttagaa tcatatcagt taatgatgga tgtcattaac cacaaggtaa
     7141 gtttagacaa tgttcatgcg tctatgttag caaatttttt atataatgat tggctaacaa
     7201 acgatgaaaa aatagtatct gggataggat tttcagctca tagcgccggt attttcaatg
     7261 tgttatatgc tagtggtagt actagttttg aaaacattat tcactttatt aaacgtcgtg
     7321 cccaacttat agaaaattgt catgtgtctg aagagctttg gcttttgatg gttgaaaaca
     7381 ttgatatgtt tagtaaggat gttttaaaag aacagtctga gaaagtaaaa ttagctatta
     7441 ttactgatga tagagctggt gtaattgcta tggatcaagt caaccgttct ttctttgatg
     7501 acgtgatgac taacaaaggt tatttttata aattaaagca gttaggcgta aaagcaccat
     7561 atcatactga ttttttaaat gaatctaaag agcagtataa acaattgatt gaaagcctag
     7621 atatttcgca aaatgatgac tatgactata tttttcattg tgatcaattg gtagatgaaa
     7681 ttttatatca gtggaccaat ctctttaact ggcgtgcgat taaggaaact atcatagaaa
     7741 aggatgcgga gattattgat atttctccta ataggtttat ttctagtcag ttgcaaaaaa
     7801 tgaaaaagag ggggaggaaa cagttatgaa agtagcaata gttactggtg gtaccagagg
     7861 aattggaagg gctattacaa aagagttata caaagaaggt tacaaagtca ttgctattta
     7921 taatagcaat gatgctaagg caagagcttt acaagaggag ttaccaaaat tagacgttta
     7981 taaatgcaat atttcagatg caaaagcagt acaaaaattg gtgactaaaa tttttagaga
     8041 atatggcggt attgattgtt tagttaataa tgctggtatt gttagagatg gctttttctt
     8101 gatgatgtct aaggaaaaat ggatggatgt cattaacata aatattatgg ggctagttaa
     8161 catgagtaaa gccgtgttaa aaatcatgaa ggctaagcgt atacagggaa aggttatcaa
     8221 tatttcttca actagtggta tagcagggca gatagggcaa gcgaactatt cagcaacgaa
     8281 aggcgctatc atttccatta ctaaaactct agcgaaagaa tttgcatcag atggtattac
     8341 gattaattgt gtaagtccag gattcattga aacagatatg acaaatgaat tgcaaaataa
     8401 agaagagcta aaagagcatc ttattcctct aaaaaggttt ggacaacctg aagaggtggc
     8461 ttggctcgta agctttttag caagtgagaa ggcaaactat attacaggaa aaaatattgt
     8521 gattgatgga ggaatgatta atgattaact gggctgaaga acgtaaaata ataaaaacac
     8581 gtcaagaagt agttgatgaa attaaaggta ttttgattga ctctttaatg ttggatattg
     8641 ataaagatct gattatgaat gatcaaccat tatttggtcg tggattagag ctagactcta
     8701 ttgatgccct agaactgtca atagggattt caacaacgtt tggtgttgaa ctaaatgatg
     8761 atgatatttc aatattatca tcagtaaata gactagcaga ttttgtgatt gaaaatagtg
     8821 aggatataga tgaccaagct aagtagtgaa gaaattataa aaatgttacc tcatcgctat
     8881 ccgtttctaa tggtggatag agtgcagagt tataaaaaag gtatcattga atgtcgtaag
     8941 aatattacta tcaacgaacc ttactttcaa ggacattttc cacaacgtcc tatagttccg
     9001 ggtgttttga tgattgagat ggcagctcaa tctgcagctt tactttatat tttagatgca
     9061 cttgatggta gcctcccctc tcttgatgaa ttaacagcag agaaaatagc agaaaaagta
     9121 ggttatttag ccagtgtgaa gaatttcaaa ttcaagaaaa ttgttactcc aggagaccag
     9181 ctaaacatta catgtaaatc tcaatcaaaa ttggggcatc ttttagaaat tcaagtcgtt
     9241 attagagatg agaataaaaa agaggttgcc tcaggaagga tgttggtatc agaaaatgga
     9301 aattaaactc aaaaatattg gaaaaaaata tggttctttt gaagcattga gagacattaa
     9361 tctcatcttt gaagagggaa aattttatgg ccttcttgga cctaatggag ctggtaaaac
     9421 aactttattt aaccttttaa ttcaaaattt taagcaaacg tcaggcgata ttaagtggga
     9481 ggtggatgga aaacctctat ctattaaaga tttttatcgt cacattggta ttgtcttcca
     9541 aagtaatcgc ttagatgata atttaacggt tgaggaaaat ctaatttcta gaggtgcttt
     9601 gtacggttta tctaaatcac aagtacgtaa tcgccttaaa gatcttcaaa cgtatctaga
     9661 tatcacagca ataaaaaaac aaaaatatgg cagtttatca ggaggtcaaa aacgaaaagt
     9721 tgatattgca cgcgccttgc tgccacaacc ttctctatta ttattagatg aaccaacgac
     9781 tggtttagac cctcaatctc gtcgcgattt atgggatgct atcgcacaac taaatcaaca
     9841 atcgcagatg acagttgtac taattactca ttatcttgaa gaaatgtctg cttgtgatgt
     9901 tttaaatgtt cttattgaag gaaacattta ttattcggga gatattaaaa gctttattga
     9961 gcaacattcc acaacaaact tgaatgtggt attgaaacca gaaaagtcat tagaccaact
    10021 ttcgatagct gattttgtta ataaatgtca agtcctttca gaaagggaga tagtatttaa
    10081 ggatatttct gttgaggaaa tgatgcaggt tatctcagac aatcaagata aatctattat
    10141 tgagacattt aatgttgaat attcgaactt agaggcggcg tatctcaact tgcttagaga
    10201 aaaagaaagg aaaaacaatg tttgaactaa tttctagaaa tcgtaaagtt tacacgcgag
    10261 atcggttagc gtttttcatg tcttttttat cagttattat cttaattttg gtctatcaga
    10321 ttttcttggg aaaaattcaa ttagatgcta taaaagcagc attgaggagt aatacagtat
    10381 ccagtgatac tattcacatg gttaattatt ggctcattgc aggtttgacg actattattt
    10441 caatgactag tacattaggt gcctttggag ttatggtgtc cgacaaagaa aaaaaattaa
    10501 atgaagattt taaggtcagt cctatatcaa atatcaaaat tgaattgtcc tatgctatat
    10561 ttgccgtttt atttggtatt gtcatgacga tgttctcttg catctttgct atcggtattt
    10621 ttaacggctt taattccttg ctagagtatt cagctatgga ttatttaact attttaggga
    10681 tcgtttcctt agggactgtt ttatctgcgg cgatgatttt acctatttta gcctttattc
    10741 atactagttc agcttttacg actcttagca caattgtagg gacttttatt ggttttattt
    10801 caggagtata tctctcaatt ggctcggtag gaaaagcatt acaacaggtt atgacatggt
    10861 ttccattgac tcaaatcaat tctcttttga aacaggtctt aatgaagggt tctattgcga
    10921 aggtatttga caaagccaac gaagccactg tctctaacta taaagaatca tatggtgttg
    10981 ttttgcgtaa tgctgatgga gaaaggctga gtaatcactt tatgttgatt tatatcattg
    11041 ccctcattct tattttattg gcaattcatt ttattattaa aaaggttaaa aaatgaaaga
    11101 tgataataaa ttaaagattt ctgaagcttc cttagaagat tattctgaag tggttcattt
    11161 atttaatagg aatcatgttt atcaatttcc agacggtagt cctttaactg ttgatgacct
    11221 tgatttaacg ttaaaagtta aagaagtgac acatttattt ttactgaaaa atcatggtgt
    11281 tttgattgga acatcagctt tctttaaatt tattacctat ggttgtttag attggaatag
    11341 tagctttagt ggttttcttt taattgattc gaaaagtcgt agtggacagg caatcactta
    11401 tctatataaa actatcttaa agaagataac aaaactgaag ttttctaata tctataccga
    11461 aatcagtaac tacaataaac cctccttagc tttatcaaaa ttgaatggct tcaaagagta
    11521 tgataagaca tatgaagaca tactgcattg tcgatcattg cgtagtcacc tccctaagat
    11581 attgaatacg tttcgtattt cgaattatta tggtaaaaca tacgacatat caacttttca
    11641 aattatggag gaaattgaaa atcccttgga agaggagaca gaaattagga ccaaagtctc
    11701 tgatgaagaa atattattta aagcagaaga tagtgcttca cttccttatt atttaaaaat
    11761 gagcttgttt caaatggaaa ttgctaggtt agataaccgt tatgttttac aagttgactt
    11821 tttatcggaa caagtaaaga gggttcgggt taaaactgga aaatatcact tggcaaactt
    11881 aacgagggct caccctagcc taactttaag tcgttttgcc aattattatt acattcaagc
    11941 aactgtggaa accctttatg gtaatatcga tgttcaactg gaacgtcgaa aaaagcatta
    12001 tagagatgca actatttgtc ttaagagaac ttttcaaggt tatgatttac ttatctctcc
    12061 aaatggcagc cttatttttg aaaagcagaa aagaaagata cttgaagata gttttttgat
    12121 ttttagccaa cctttggaca agaaattagt tgtaaaagaa gaagaaaatc acattacaat
    12181 caaatgtttc tatcaaggtg ctttaattga gaaaatcatg acatttacaa gtgacgaaga
    12241 aatcacttgt gtttataaat gtaatcaaaa ggctaaagag atgttcccaa aattattgaa
    12301 acagactttc aagttacact gccaagagca gttgattaga gatagtgaag gttatcttgt
    12361 gaatgttcca ggaagttacc cgattgagca tgatgatttt cttcgagcag ataagtttga
    12421 agatagacaa ttccattatt acctcccgaa tgaagataaa atgatttcct attctcctcc
    12481 tggcaaagcc agcaatcaaa tgcaatttag acccttatgt cttattgata cagatagcct
    12541 ttcttttcca ctgacttatc attttaggat ttcacaagca tcatcggagg aggcgttaat
    12601 caatctaaaa aaacagccaa tatgggacag caactaccaa gcgtcagcaa ctgacctttt
    12661 aaaacatatc tctaacctaa cattagaaga agagaaggat tatggcatca agagaatgat
    12721 agctaacaga aagcattatc ctagtcacaa actggtttta gcctataatc agattgtttt
    12781 gcctaagaat gaaattccaa gagacagtga attatattct atttcttttg attatcgaat
    12841 tagaggaaag tttgtccaaa tccgccaagg ggaacatgtc aaatatgata ataaatccta
    12901 tgttttggaa aatagtcaac aattagtatt gtatgtagct agcgatgata aatatatact
    12961 aatttctgct aaaaatggaa tattttattc ttacaaagag aacaatcatt taaaaataag
    13021 gtgcatgttt aaaaggaact caccttatgc tacaaatgtg agcattacag aatatagaaa
    13081 gtgtgaagaa aaatgaatac tatttataat acattgagaa cagataaagg ttataaagtt
    13141 tatgaggggt atttatatga aattactggt gaagaatgtg aagaagcctt agaccttgtg
    13201 attcctaaga atattgtatt tgcagataca gatacttgtg gctacacttt tttactcaat
    13261 gaagatggaa cagtttatga tgatgtgact ttctacaaat ttgatgataa atattggttg
    13321 gctagtcata aagctttgga ttcttattta gacaacatca attttgacta taccgtaaca
    13381 gatatttctg acgagtataa aatgctgcaa attgaaggaa gatattcggg agaaattgct
    13441 cagtcatttt atgaatatga tatttcaaca cttaattttc gtactttgat agagatgact
    13501 tataaaggtg agaaaggtta tcttgctaga tttggttttt ctggagaatt tggctatcaa
    13561 tttttcctac catcttctat ttttgctact tttgtttcgg atgtctgtga aggtatagca
    13621 gagtatgggg atgaacttga tagatattta aggtttgaag tgggacaacc cattactgat
    13681 atttatcaac aagaagaata ttctttatat gaaataggtt attcttggaa tctagatttc
    13741 acaaaggaag aatttagagg tcgcgatagc ttgttagagc acatcagatc agcaacagtt
    13801 aaaagtgttg gattctcaac gaaggaaaaa ctcgcttcag gaacaccagt gctatttgat
    13861 gaccaaattg ttggaaagat tttttggata gcagacgaga aagactcttc ggaaaattac
    13921 ctaggtttga tgattgttaa ccaaacatat gctcattcag gagttacttt tgtaacagaa
    13981 gatggccaaa ttttgaaaac acaatcaagc ccttattgta tcccagaaag ttggaacaaa
    14041 gaatgagcgt atatgttagt ggaataggaa ttatttcttc tttgggaaag aattatagcg
    14101 agcataaaca gcatctcttc gacttaaaag aaggaatttc taaacattta tataaaaatc
    14161 acgactctat tttagaatct tatacaggaa gcataactag tgacccagag gttcctgagc
    14221 aatacaaaga tgagacacgt aattttaaat ttgcttttac cgcttttgaa gaggctcttg
    14281 cttcttcagg tgttaattta aaagcttatc ataatattgc tgtgtgttta gggacctcac
    14341 ttgggggaaa gagtgctggt caaaatgcct tgtatcaatt tgaagaagga gagcgtcaag
    14401 tagatgctag tttattagaa aaagcatctg tttaccatat tgctgatgaa ttgatggctt
    14461 atcatgatat tgtgggagct tcgtatgtta tttcaaccgc ctgttctgca agtaataatg
    14521 ccgtaatatt aggaacacaa ttacttcaag atggcgattg tgatttagct atttgtggtg
    14581 gctgtgatga gttaagtgat atttctttag caggcttcac atcactagga gctattaata
    14641 cagaaatggc atgtcagccc tattcttctg gaaaaggaat caatttgggt gagggcgctg
    14701 gttttgttgt tcttgtcaaa gatcagtcct tagctaaata tggaaaaatt atcggtggtc
    14761 ttattacttc agatggttat catataacag cacctaagcc aacaggtgaa ggggcggcac
    14821 agattgcaaa gcagctagtg actcaagcag gtattgacta cagtgagatt gactatatta
    14881 acggtcacgg tacaggtact caagctaatg ataaaatgga aaaaaatatg tatggtaagt
    14941 ttttcccgac aacgacattg atcagcagta ccaaggggca aacgggtcat actctagggg
    15001 ctgcaggtat tatcgaattg attaattgtt tagcggcaat agaggaacag actgtaccag
    15061 caactaaaaa tgagattggg atagaaggtt ttccagaaaa ttttgtctat catcaaaaga
    15121 gagaataccc aataagaaat gctttaaatt tttcgtttgc ttttggtgga aataatagtg
    15181 gtgtcttatt gtcatcttta gattcacctc tagaaacatt acctgctaga gaaaatctta
    15241 aaatggctat cttatcatct gttgcttcca tttctaagaa tgaatcactt tctataacct
    15301 atgaaaaagt tgctagtaat ttcaacgact ttgaagcatt acgctttaaa ggggctagac
    15361 cacccaaaac tgtcaaccca gcacaattta ggaaaatgga tgatttttcc aaaatggttg
    15421 ccgtaacaac agctcaagca ctaatagaaa gcaatattaa tctaaaaaaa caagatactt
    15481 caaaagtagg aattgtattt acaacacttt ctggaccagt tgaggttgtt gaaggtattg
    15541 aaaagcaaat cacaacagaa ggatatgcac atgtttctgc ttcacgattc ccgtttacag
    15601 taatgaatgc agcagctggt atgctttcta tcatttttaa aataacaggt cctttatctg
    15661 tcatttcgac aaatagtgga gcgcttgatg gtatacaata tgccaaggaa atgatgcgta
    15721 acgataatct agactatgtg attcttgttt ctgctaatca gtggacagac atgagtttta
    15781 tgtggtggca acaattaaac tatgatagtc aaatgtttgt cggttctgat tattgttcag
    15841 cacaagtcct ctctcgtcaa gcattggata attctcctat aatattaggt agtaaacaat
    15901 taaaatatag ccataaaaca ttcacagatg tgatgactat ttttgatgct gcgcttcaaa
    15961 atttattatc agacttagga ctaaccataa aagatatcaa aggtttcgtt tggaatgagc
    16021 ggaagaaggc agttagttca gattatgatt tcttagcgaa cttgtctgag tattataata
    16081 tgccaaacct tgcttctggt cagtttggat tttcatctaa tggtgctggt gaagaactgg
    16141 actatactgt taatgaaagt atagaaaagg gctattattt agtcctatct tattcgatct
    16201 tcggtggtat ctcttttgct attattgaaa aaaggtgata aaatgagtaa atttttattt
    16261 gctcctttca tgatgaattt aggagagagc caaagattat caaaactggc taattgttta
    16321 tatgaacaag gacatgatat tcatatcctt ggagacaatt attatccatt tttatttaat
    16381 aatgatgctt atcgctatca ccactgtgta gaagataaat ctgtttataa tagtaagcga
    16441 tatgatgctt tcttttctct ttcaacagac tttaattttt tatcagaaga agaaattgaa
    16501 cgcatctgtt ctattgaaag agacttgtta cgccaagaaa aatttgatgc tgtattgaca
    16561 ggttaccgtc tatctattgt tacaagttgt cgtttagaaa gcataccatt aatttggatt
    16621 atttcagggg caacgcatat cagtgaaatt gttgaaaact cagaaggaat attgcctaat
    16681 gggaagataa gtaaagctag taagcctcaa acaaaggatt ttatcaaaag agtaatcact
    16741 acttattcaa caaatgttaa aacgtggaat aactatatta aaaaatatgg tggcaaacct
    16801 tttaataatg ctttagaatt atttactggt gatctaaact tggtaacaga ttactctttg
    16861 ttttatgaat ttgataaaga ttcgtcctat aaaacgatag gacctatttt gatagataat
    16921 gtaggttttt caaaatgcag ccaaattaat caagacaata agactgtact gctcagcttt
    16981 ggtacttcat ttaaacgaga ttgggtggaa tcttttttaa agacattacc aaggcattat
    17041 cattatttac tgacaagctg tggtgaggct ataaacattc caggaagtta tattgagata
    17101 gttgatttta ttgattttaa aaccatagat aaagagattt atttcgctat tatacacggt
    17161 gggcaaggaa cagtttatgc gatggctgca caaggtattc cctttatagg tatccctttc
    17221 tttaacgaac aattttggaa tattaagaag ttcagtaaag aaaagtgcgc ttatttggta
    17281 aagaaacctg atgtaagaga gatacgtaaa gcaattagct gtcttgaaag agactatgat
    17341 atctataaaa gttatatgat aagactaagc caaggaatta aagaagaagc agaggcatct
    17401 ttacaaaaag cggaggcaga aattgaatcc tttatcagaa aaagacaaag ttaactatat
    17461 tatgatttat aaaaataggc agtctacatt ggagaataat ttatctggcg atcggttgat
    17521 gagatatcaa caagcaaata gacaggtaag caaagataaa ctgttaacag ggagttattt
    17581 tgtgtaccaa tgcttaaaaa agttaggttt taccaataag atttgtcagg aatctttttc
    17641 tagtgttagt agttatttaa ttggtttgcc aaaggggaaa attagttact ctaattctgg
    17701 tgactatcat attctaacct atgctcccag tggttcaact ggggttgata ttgagaaata
    17761 caaagataga tcagaacaaa cctaccaaaa ctacctagga gaatcagtta gtagtgatat
    17821 ggattctaaa ttattatttt ataaggcatg gttacaaaaa gaaatttcct ataaatgtgg
    17881 gaaatctata gatattacct atcaaacaat gatagatggc tatatttatg gttatgcttt
    17941 tgataatact tttaaagtag aagcggttta cttaaaagaa ttattaccag ataactaact
    18001 cgcaacatgt tgttcctaca tatgcatcta taaaaagcac tttgaagtat ttcaagggct
    18061 ttttataata ccttgggtag tctttaacaa ttatggttag agttgatatg ataaactgat
    18121 catttccaca ttttatgaga aaatcagaaa atagaggtgt ttttttgtga taataattag
    18181 aaataataaa aactgaaata ttttctaatg taaaaaaaga taaaagaact tattgctgaa
    18241 aacgatttta acttaacatt tgccattgac aagtacttag aggagtgcta aaatttcgtt
    18301 agctggttaa caaaccggtt aagtaatgtg aggttctttt atgagtgaat tcctttcagc
    18361 caaacctatt ggtcataatg attcatctaa tactatttta agaattatag agaatacgaa
    18421 atatattcat cactatgatg gaaaagttgt tgtcgaaatt cctcctcaaa aagagcctat
    18481 caaagtcttt tttatggatt cacgtcttca ttatttagaa ttttcagaca tatgtcggta
    18541 tgtaactgtt gttttgaaaa atagtaccgt tgataatata accaatttag ggctagataa
    18601 tcatattgtt tatgacaatg gtgatgatat ggttgatccg ttgccgagta ttcagcatgg
    18661 attatttctt aatgatgatt aaagatgtag acaaaaattt agaaacgagg aagccatgac
    18721 attttataaa aagacggaca aatttggatt taggaaaagt aaagtgtgcc gcagcctatg
    18781 cggtgcttta ttgggtacag tagcagttgt tagtctagct acagcaagta cagagattca
    18841 cgctgatgaa gcaacaacct cacctaccac tgtaacgaaa gtacctcagc cagttcaagc
    18901 agacacaaca gctctaaata cctctaagac tcacagtaca caagcaacta caactcctgt
    18961 tgaagctaag gagaataagg ttgtcaaaag tgaaactgtt caatctgaga gccgagtaat
    19021 gcctagagat aaggtagtag aaaggccaga aacagtaaaa gctagtgtta actcagacgt
    19081 tagccaacct attacaacca cacccccaac aattaatgaa aaaacagttg agattcctaa
    19141 cttagctcaa gatactaaga aggtagctcc aaaagtaact gttacaccag aagttgttaa
    19201 tcagccaaag cagttgccta aatacgagta agaaaactca gagaaaggca ataagagtac
    19261 agaagcaact aaggcagtta ctgtgactaa accacagggg gcggttgctg agaaagctac
    19321 tccggctgtg ccaaagcctc aaaaagtaag agtaattgtt gaatttaata aggaatctat
    19381 cttagattat gcgacagaac agaaaaaaac agttgcacag ttaaatcaag ctgatgttga
    19441 gaaaaaatta caaagcatca agcaagagca agataaagtt cttaagaata ttgaaaaaag
    19501 tgttcacttt gattcaagca aagtgaagcg ttacgatgct attattaatg gtgtcgcact
    19561 tgatattcaa gctcaagaaa ttgaaaaatt aaaaacgatt gctgatgtta gaagggtcta
    19621 tgtctcacaa gaatatgttc agacaaaacc tttactatct tcctcagggc aacttattgg
    19681 tttgccagaa gtttggaaca actcccagta taaaggtgag ggaactgttg ttgcagttat
    19741 tgattcaggg gttgatttca aacaccaagc actaaaaatc aaagaaccaa atcgtgctaa
    19801 gtataataaa actagtattg aaaaactgat tcatgaaaag aatctcaaag gaaaattcta
    19861 ttccgaaaaa gtaccttatg gttataacta ttatgactat aatgataacc tcaaggatag
    19921 ctacggtgta atgcatggta tgcatgtaac aggtatcgta ggagctaatg atgataatca
    19981 aaaattgtat ggtgttgcac caaatgctca aattttagcg atgaaggtat tttcagatga
    20041 ccaacaaaat cccacaacct tcacagatgt gtggctaaaa gctctagacg atgctatttt
    20101 actaaaagct gatgttgtta atatgagtct tggaacacct gctggatttg tacatgaggg
    20161 gaaagactat cctgaacttg aagttattgc aagagcgtgt aaggcaggaa tcgtcattgc
    20221 agtagcagca ggtaatgaag gaaatatcac tgacggtaat acttatggtg ttaagccgct
    20281 agcagaaaat tatgatacag ccttgattgc caacccagcc ctcgatgata ataccttggc
    20341 tgttgcctct atggagaacc ttaaaaagca tgctcatgtt ttgaaattta aagataaaaa
    20401 atctggaaca gaagttacag aagtcattaa tcttcatgtt gccccaaacg cttcaaaaac
    20461 tattattgga ttggctgtag atttgggagc aggagctcct tcggaattgt caaaacattt
    20521 tgatttaagt ggtaaaatcg ccatgcttga aattccagaa gataataaat ctaatggatt
    20581 tttagaaaaa gtacaggcga ttactaagtt aaatccagct gctatcttgc tttacaataa
    20641 tgcgaaggtt aaagatgatt taggcagtca attattagtt gaatcagaag cagctaaatt
    20701 taatattgct cgcattacca gaagcacgta taacaatatc aaaaataata gcaataaaat
    20761 tattactatt ttaacagagc gtcaagctat tgataatagt ttagcaggac aattatcttc
    20821 ttattcttct tggggaccta caccagactt acgtctaaaa cctgagatta cagctccagg
    20881 tggtcatatt ttctcaactg tggaggataa tcaatatgct gataaatcag gaacatcaat
    20941 ggctgcacct caggtagctg gggcagctgc agttttaaaa caatacatta cagataaaaa
    21001 aattcctgtt gataatgctg ctgattttat taaattactc ttgatgaaca ctgcacagcc
    21061 aattatcaat aaacaatcaa aagatggcaa aacaccttac tttgtgcgcc aacaaggtag
    21121 cggagcaatg aatcttgcca aagctcttgt tacgacagtc gttgcaactg taacagggac
    21181 taacgataat aatgcagatg gtaagttgga gttacgtgaa ttaaaagaga aaaaatttaa
    21241 agcacgcatt ctacttcgta actttggaaa aactaataaa acgtatatca tctcatcaga
    21301 agcgattgcg gacccagttg atgaaaaagg atttcgtacg caaaattcag aacacctagt
    21361 atcaaaaaaa gctgatgcag ttacaaggaa agttactgta gaagcaggta agacattagc
    21421 tgttgacctt gatgtggatt attctgatgc agaggcttta actcgcaata atttcttgga
    21481 aggttatctt aacttaaaag atactgaagg tgtagccgat ttacacttac catttttagg
    21541 tttttacggt tcatggacag agcaaaaagc tattgatgct tttgaaggca tttctgaaat
    21601 tggtaacggt gacaaaaaac gtcgcgttca attctatgtt aataaagaaa ccaacaagac
    21661 ctcatcaacc tttacaacta acggcatgtt gagtcttcca atctataaca atacagtatt
    21721 tttctctcct aatagtcctt tctatgacaa agcaggagtt cgtattgcag ctttgcgtaa
    21781 tatggagtat gttcaatatt ctattataga tccagatacc aataaagaag ttcgtgtact
    21841 tggtagatca cacgacgtta gaaaacttta tcgcctagat tatcgtaata gttttgccat
    21901 gatgccagat tctatttggg atggtaagat taaagattaa attgcaaaag gggataaaca
    21961 atacatttat caaattaaag tacagttgaa taataaaggt gtcggtggcg atggtgtcca
    22021 aatttatcaa tactatatca aaatggacaa caataaacct tacttaagtc ccaaagataa
    22081 gactactgta gagaagttag aagatcgctg gaaaaaaatt actttcaaag ttcaggatac
    22141 tggcattggt ttgaaagacg tttatcttca atctgttaag tatgttggtg gtggcaataa
    22201 taatttagac cttatcacac ctccaggatt taaaaaagaa gataaaaaag ttgaaaaacc
    22261 aaaattagac cgtccaccag gaattgattt accagcacca acttcaatga gaagttttga
    22321 ttattcaacc ccaccgggaa ctaagccaag caaacccaaa gatagtttat caactcctcc
    22381 aggtttccca gatttaaaca cgccgccgga tgaagcacca aaggatagta aaaaagacgc
    22441 tattgaagat aaatcaggag caattaaata tgctaagtct cttcaactta gctttgttga
    22501 tgaccctatt ttagctagca aagtaaatgg caaaatatta caagtcgaat ctgatggcaa
    22561 attagtcatt cctagaaatg ctttgtcagc taatcaattt gatgacacta gtcttaaaat
    22621 ttatcgtaat aataatcgca ataaagaaat tactatcaca acagattatt ttgcagatac
    22681 aaaatatgtc aatatcacag cggttgacta tttgagcaat actacttttg agcaattagc
    22741 tactggtgaa acagtagatt accatgccat tgtattttca agctttgctg ctattaaaga
    22801 caagggtggt aagatttatg ttaacgataa attgcaagaa acttctcgta tagcgcttaa
    22861 agataaatct gttaagattg gtattgaatt accaaatgat gtcagacata ttgatagttt
    22921 atctgttcgt cgtttgaatg aggttaaaac tgttgataat atcttgaaaa atgatgaaca
    22981 agacattaat ctcagcaaaa cttaccaatt aaaatacaac ccgacaaatc gtcgtctaga
    23041 gtttactatt aataacatta actcaagttc agaaatcatg accactttca aagatggaaa
    23101 gatgccagaa ttggttgaac aaaaagatgt ttctttggat ataaacgata tggacatgag
    23161 taagtttaaa actattcgac ttggacgaaa ggattctgaa tttaagggac aacttattgc
    23221 aaaaactgga acagttgaat tagatatgtt tttcaaacaa tctcaagacc cagcttcaat
    23281 tattaaaaaa atatacctta tccaaaatgg tgttccaaat gaattgaaaa aatttgactc
    23341 tagttttggt ttaactgaaa gtcagataga tggatactat atttataaag atgcaattaa
    23401 ccttaaattt aaattaacca gtggtgcaag tcttaaagtt gtttataaag ggcaagaaga
    23461 tccatatagt catcagaaag aagatatgac taaaaaaggt gaacagctca gtcattcaac
    23521 tcaagccaat gaaaatacag caaaagtaac ctttgctaat attgactggt cacattatag
    23581 taaggttact gtgaatggaa aagaagttgg taaaggtagt gagttacctt taactaaagg
    23641 atggacaaca tttgtattac ataaaacaga aaattcatta aatgttaaaa gtttgattat
    23701 ggagacgggt agtgtaagta agaaagttca acaacttcct ttaagtccta gattatctaa
    23761 aaataagcat atgagggata tgctacttac tatgcaaaaa gattcagcgt attacgaaac
    23821 aagtgacagt ctagtccttc gaattaatct cactgcagat actaaactta attttaatgc
    23881 tgttaaagga gcgagtgctc ttactgaaaa tatgatgatg agacagtttg cagttgctgg
    23941 accacaagat gatcctgtta gtgaacataa atacccatca gtatttctct taactcctgc
    24001 cttattggaa actgctagtg aggcaactct aaatggtaag gaaatcacag catctggtat
    24061 tatcggtcac atcaaggatg gtgataaaag caagcatgtt gaagtcaaaa tggtgaatga
    24121 aaatggagac atgctaggaa cccctgttat tattcaaggt aaagacttga ctaatcgaac
    24181 aaaaccatta atgagtggac gtagagtact ttatgccggt aaacaatatg agttccgggc
    24241 taaattacca cttagtcgtt ttaacacttg gattagggtt gaagtggtaa cagaagcagg
    24301 agagaaagca agtattgttc gtcgcatgtt ctttgaccaa tcagttccag agcttaacac
    24361 agcagttgct aaacgtgatt tgacttctga tactgctctt atccacatcg ttgccaaaga
    24421 tgactctcta aaactaaaat tatatcaaga tgattcatta cttgaatctg ttgataaaac
    24481 cggtctttat agttttagaa atggtgtaga aatcactaaa gatatgacag taccactaga
    24541 atttggagat aatattatta agttatctgc tgttgactta tcaaattatc gtcgtaatga
    24601 gacccttcat atctatagaa accgttttga tgttaaagca agccaaatga cagctgacaa
    24661 aggagctaaa gtaactgtgg atatgttgat gaagcactta gttgttccag aaatggcagg
    24721 agcttataca ttaacaatcg acgaagctcc aaacacaaat gaatcaggaa tgttaacaaa
    24781 cgctaaagta tcgattcatt atgtaaatgg tggtgttgat aaagttgatg ttccgattaa
    24841 agtagttgac ttagaagcta ttcgtaaagc tgaagaagca cataaagctg acgaagcacg
    24901 taaagctgaa gaagcacgta aagctgaaga agcacataaa gctgaagaag tacgtaaagc
    24961 tgaagaagca cataaagtcg aagaagcacg taaagctgaa gagggacata aaacccaaga
    25021 agcacctata gttgaagaag gctacaaggt taataacgtt catcaaactg atactacagt
    25081 taaagcgtct gatttaccaa agactaagac agtttccgca gttcatatgg ctagaacaga
    25141 caataaacag ataacttcac atcagacaca tgttgaaaaa caaattaaaa atacattgcc
    25201 atccactggt gacagcaaac gtggttatta tatcactgga atggctatcg ttatgctgag
    25261 tgtattattt agtttagcta aaaagtttaa aagcaaatat taatgataaa ataaaatcta
    25321 tttagcaaca ttaattgtga tggataaaag gttttatctc ttaaatcaaa agtagtagct
    25381 cattagctac tacttttttg tataagtgta aaaaagtgaa ttcttaacaa gaatttaaca
    25441 ccctacttgc tatactaaaa gtgaataaga aagagaggtg tcatttatgc aaagaaaatc
    25501 ctatttaaaa tccatgagtg ttcttacttt aacagcttgt cttatatcag gatatgtggt
    25561 taaagatatt gctatgttac atgcagtatc tgccagtgag aagaaagcaa ataatgtcag
    25621 tccgagagaa aatctctaca gggctgtcaa tgataattgg ctagccaata caaaactcaa
    25681 acaagggcag attagtgtta atagtttttc agaaattgag gataaattaa agcaactgtt
    25741 agtgtctgat atggctaaaa tggcctcagg aaagattgaa acaaccaatg atgaacagaa
    25801 aaaaatggtt gcatactata aacaaggtat ggactttaaa acaagagata aaaatggtct
    25861 caaacctcta aaaccagttt tacaaaaact tgaagcagtc tcttcaatga aagactttca
    25921 aagtttggcc catgattttg tgatgagtgg ttttgtttta ccatttggtt tgactgtgga
    25981 aaccaatgct cgagataata gccaaaagca attggtgctt cgtcaagcac ccgcattact
    26041 tgaatcacct gaccaatata agaagggcaa taaagaaggt gaggctaaat tatcagctta
    26101 ccgtacttca gcaatggctt tgcttaaaca agctggaaaa agtaacattg aagatagaaa
    26161 actagttaaa caagctatag catttgatag actcttatca gaaaaaacgc aagttgatca
    26221 aagtaaaatc acagctgaaa gtgagacagc tgcggggcga tataaccctg aaagtatgga
    26281 aacggttcac aattacgcca aggaatttga ctttaaagaa ttgattgaaa aactagttgg
    26341 gccaacgaat aaggcagtca atgtagaaga taaaacttat tttaaacagg ttaatgatgt
    26401 tataaatagt aaacaattag ccaatatgaa agcatggatg atgatttcta tgctagttga
    26461 tcaatcagat tttctaggag aacaaaatcg tcaagcagcg agtgctttta agaatgttgc
    26521 gtctggtttg actcagattg aatcgaaaga aaaaatgctt acacccaatt agaatatact
    26581 tttggccata ttttgggtga ttattatggt aagaaatatt ttggtgaggc agctaaaaaa
    26641 gacgtcgaac atatggctaa gaaaatcatt aatgtctata aaacacggtt aaaaaacaac
    26701 acttggttat cagaaaatac aaaagcaatg gccattaaga aacttgataa catgagatta
    26761 atgattggct atccagaaga ttatcctgat ctttatcgtc agtaccaatt tgatagtaaa
    26821 gcaagcttct ttgaaaacaa tgataactac agaaaattat cgaacaagaa aacatttgaa
    26881 gaatttaacc agtctaatca acgtgaacat tggcaaatga gtgccaatgc tgtaaatgct
    26941 tataatgatc ctaataccaa ttccatagtc tttccagcag cgatttttca atcaccactc
    27001 tacgataaaa ctaaaacagt tagtcaaaat tatggagcta tcggagcaat tattggtcat
    27061 gaaatttcac actcatttga tattaatggt atgaaatatg acgagaaagg gaatcttcac
    27121 gattggtgga ctaaagaaga tttaaaacat tataagaaaa aaacacaagc tatgattgac
    27181 caatgggatg gccttaaagc agatggcggt aaagttgatg gtaaattaac tttagcagaa
    27241 aatattgcag ataatggtgg tgttatggca tctctagaag ctcttaagac tgaaaaaatc
    27301 caaactataa agaatttttt gaatcatggg caagtatttg gcgtcaaaaa gcaaccaaag
    27361 aacaaagtaa gtcctcaatt cagtcagatg ttcatgcacc atatgaattg agagctaaca
    27421 tcccagtacg taatttccaa gaattttatg atgcctttgg tgttaaaaaa ggcgattcaa
    27481 tgtatctaaa accagaaaaa cgtttgacac tttggtaaag ctatgttttc cagacaataa
    27541 ggagaatgca tgaagaatat aacaaagcta tcaactgttg ctttaagcct actactttgt
    27601 acggcgtgtg ctgcatcaaa cacgtctaca tctaaaacac agtctcatca tcctaaacaa
    27661 actaaactca cagataagca aaaagaagaa cccaaaaaca aagaagctgc tgatcaagag
    27721 atgcatcccc aaggcgctgt tgatttgaca aaatataagg caaaaccggt caaagatcat
    27781 ggtaaaaaaa tcgatgttgg tgatggcaag aaaatgaaca tttatgaaac tggtcaggga
    27841 aaaattccaa ttgtttttat tcctggtcaa gctgagattt cgccacgcta tgcttataag
    27901 aatttaattg agcggttaag taaaaaatat aaaatttata cagtagaacc attaggctat
    27961 ggtctcagtg atattcctac taaaccacgt acgctagaaa acattacaaa agaaattcac
    28021 acaggcttaa ataaaattgg agttaaaaat ttctaccttg cagctcactc acttggcggt
    28081 atgtattctt taaattatgc taaaaattat ccagaagaag tcagaggatt tattggtatg
    28141 gataccagta caccttggat ggaaggggaa caaaaaacaa agtatgaccc tgaaagtgct
    28201 aaatgggcaa tgaaattacc agatgttgat gataagacta acgaacagta tctgtcaatt
    28261 gcgaaaaaaa ttaacggcaa taagtctcaa cgagatgaag atgagcgctt aggtgataat
    28321 cttggtaaat tgaagaatgt aaaattccct aaagggatgc caactaagta ttttcttgct
    28381 gatcaatctg tggagggaat caagctacgt caagcagatt ttaaaggttt gaaaaattgg
    28441 gatcagcagc atattgattt atctgaagat ccaaaagatg cttccgttga aattcttaaa
    28501 ggtagtcaca atattttcca aacacaatat gaacatatgg ctaaagctat tgatggattt
    28561 atccaatccc atcaaaagta atcgttgaat gaaataccta agtcaaggag aaaaaatgaa
    28621 taaaataacg aaattatcaa ccgttgccct cgctttgatg ctttgcgttg gatgttctgc
    28681 caataaagat aatcaaaaaa ctaaaactga gcagcctaag caagaaaagt caatcgctaa
    28741 gcaaaaacaa gagagagaag cagaaaaagc tatgattgct aaaggtgcta ttgacctaac
    28801 taaatataaa gcgaaaccaa ttgaagatta cggacaaaaa attgatgtcg gtgacggtaa
    28861 aaaaatgaac atatatacaa tgggtcaagg gaaaattccg ctagtagtct tccctggtca
    28921 agctgaaatt tcaccacgtt atgcttacaa aaacttgctt gatagattag ggaaaaaata
    28981 taaaatttat acggttgaac cgctcggtta tgggcttagt gatatgacag ataagccacg
    29041 tacgattgaa aatattacca aagagattca tacagctctt gaaaaagtcg gtgtcaaaaa
    29101 tttctatctt atggctcact cgttaggtgg catatacggt ctaaattatg ctaagacata
    29161 ttctaacgat gtcaaaggtt tcattggcat ggatacaagc acaccaaata tggaaggtga
    29221 tggcacaact ggagaatctt cagcttcaac cttaaaatgg gcagaatcca ttccagaagt
    29281 tgataagaaa accaatgaac aatacttatc aatcggtgct aaaaaatcag gtaataaaac
    29341 agagcgcgat gaagataggc gtgttagtga caatcttcat aaaatggaaa aagttaagtt
    29401 ccctaaaggt atgccagcta agtatttctt agctaatgag tcagcagaag atataaaaat
    29461 gcgtaaaaaa atattcccta agcttaaaag ctgggaacaa caacatattg acttatctga
    29521 agatccaaaa gacgtttctg ttgagacact agatgctagc cacttgattt atcatacgca
    29581 atataaacat atggcgaagg ctattgacga gtggattcaa tcgcataaaa aataattatt
    29641 ccccccaatt attttactaa ggagaaaaga tgaataaaat aacgacatta tcaaccatcg
    29701 ccctgacttt aatgctttgc gttggatgtt ctaccaataa agataatcaa aaaactaaaa
    29761 ctgaggatcc taagcaagaa aacgtaactg caaattcgaa ggaagacaag gcaacagcag
    29821 ccaagcgaat gaagttaacg aaagaacttg aaaaatataa taaaggcaca gagagcttta
    29881 ttagtaacaa acaatatcaa aaatatatca aaacacctat caaagaatat ggtcaaaagg
    29941 ttgaagtcaa aggcaagaag atgaatgtct atactgttgg agaaggtaaa gtaccaattg
    30001 tctttattcc tggtcaaggg acggtgactg ccaaacatca gtaccataat ttgattagta
    30061 atttaagtaa aactcataag gttgtggtag ttgagccatt tggttcaggt ttaagtgatg
    30121 tcattgacca accacgaaat ctagctaata ttacctcaga tatccacgaa gctttacaaa
    30181 aagttggtat taccggaaaa tatgtaattg cctctcattc aattggtggt gtttatgccc
    30241 ttaaatacat cagtacttat ccaaaggaag tgctaggatt aataggttta gatacatcaa
    30301 cgccgggtat ggaaggtggt aagcaagttg atttcgctgc tccagtgtta aaagaattac
    30361 ctaaaattcc taaagtatcc gacgatatta atgcacaatt ttttgctatt ggacataaga
    30421 tacttaataa ttctaatatg aaagaagaag cgaagaattc ttctaatatg attaacgaaa
    30481 gtgccaacta taagattcca aagggaattc ctgctatgta tttattagca cgtgaaagtg
    30541 acttagggct tcaattacgt agtttagata taccaattaa aggcagttgg caagagcaac
    30601 ataaggagtt atcagaagat ccaaaagaag tctctattca tacactagac ggaactcacc
    30661 aaatttatga tactaacctt aaagaagtaa ttgattatac taaccaattt atgaaaacat
    30721 ttgagaaata attgttttta gaaacagtgc tataaaatca ctaattgata gataacgtag
    30781 aagtcgatag agaatatcta acgacttcta tcgttattct agagattatt aagatagaaa
    30841 aaggagggaa aatagtgaac ctatggacat taagtttagc ttatttgaaa cgtcaaaaaa
    30901 tgaaaacagt aaccttgttc ttagtttttc taactatagg cacatgttta atcagtctga
    30961 tgagtattca gcattctttg gaaaaaaata tcctaacaaa acaagggaaa agcatttatt
    31021 taacctccaa agaaaaggct tattggcctg aacaagcata cgaagctttg aaaaaagcaa
    31081 aaatggttga gtcagtagag gcaagtttaa gcattgatgt tggtagccgt ttaaaatctg
    31141 tatcaccata taactcttct aaagaagaga atcaagttac attagcaggt tatcaaagta
    31201 cagaagattt acgagctttt cagacaaaag cattggtctt aaagaaagga agccatttag
    31261 cggccgataa tacaaaacaa gtactagttt cattgaaatt agcacaaaag aatcatttaa
    31321 gtgtgggtaa caaattgaga ttaggcaaag aaaatgttac tattgcagga atttatgatg
    31381 cgaatagtgc taagtctaaa aatactttta atcccaatat tgataataca ttgattgctc
    31441 aggcaacatt ggttaggaag attagtaaac aaaaaggtta tcagacggtt gcagtgcgct
    31501 taagtgataa acgtttagtg gatacagtta tccaaaacat caaacaatgg ccgcttgatt
    31561 tcgggaaatt agatgttcaa acagctaaag agttttatgg agattcttat cgtaatatcg
    31621 agacattgca tcgtctagtg ggacgaatca ttcttattgt tagtctcgtg gcgatggcaa
    31681 tattagttgt catgctaaca ttttggatta ataaccgcat taaagagacg ggaattttac
    31741 tcgctatcgg aaaaactaag tttgaaatca taggtcatta tctgatagaa gtactgttag
    31801 tagcaggtgc tgccttcaca ctatctatta ttggtggtgt ctttcttgga aaaacatttg
    31861 cagctggcct tttatcacaa gttaatggtg gtgtgagttc acaaattgta caaaatagta
    31921 gtctaattat tgatagaatt gataacctcg ctgtttctgt tggagtaatg gatgtcttta
    31981 gactctatgc tcagggtgct ttaatttgct tatttgcagt tgtattatct agttattcta
    32041 ttcttaaact acaaccaaag caaatattgt cacgaatgtc ataggaggtt aatatgaatt
    32101 tatttaaacg ttccttttta tatgttagcc gtaaaaagcg taagagtatc actttattcg
    32161 tatgtttatg gctagttgct tctacattaa ttagcggtat tgcggtaaaa aatgctggtc
    32221 ttactgctaa gaagacattt tcaaggcaaa ctggatcaat tttgcatatt tcatcggata
    32281 gtactgacct tgttggtgat ggttacggat ctggagaaat ccctgaaaaa gcaatagtta
    32341 acatagcaag taatctaaat gttaagcgcg ttaataataa tttgatggct tatgctggac
    32401 taacctcgga aaaaatggtt acacgaccaa atgataaaga acaatataaa gaacaagttt
    32461 tacaagttca tggtaatagt tactccgata cagatcctaa atacacagcg gggatgattt
    32521 ctcttaaggg agggcgacat atcacgccga aagatcagcg agttataatt gttcatcagg
    32581 cttttgcgaa gacaaatcat cttaaagttg gcgataagtt aatgttttca aaagaccccg
    32641 ttagaaacag taaggataaa acattagttg aagcaactat tgttggcata tataaaggta
    32701 aaacggtaca aaaatcaaat tacccttatg agatgctaga aaatacagcc ttttcagata
    32761 cgcaattgat taaggattta tacggctatt ctaaaggtaa ggccttttat cgccaaacaa
    32821 ctgtatatcc taaaaagaat gctgatatag aaactcttaa aagttttatc aaaaaacaat
    32881 cctattgatt ggaataaata ccaactgaca attacagcaa ccttgctaac aagttatgca
    32941 aaaagtatag atgtcttgaa tcaattaatt acttctctac aaggcggcat gttagtagta
    33001 tctattattc tagtgatgat ggctctgcta ttctggattg gtggtagaac ccatgagaca
    33061 ggtattttat tagctattgg taagtctaaa gcaattatca ttgtcagtac gcagtagaag
    33121 tactaatgat tgctattcca gcctttgctt tatcagtact tatcagtcaa tttattggtc
    33181 aaaaattagc agactcctta gtgtatcagg cctctagaac tgtcagagca gactttatga
    33241 aagggtttgg gaatatgcag cttgggtctg atagtgatac agatatgttt atgcaaacga
    33301 aatgtacaag tgaagcctga ggaagtctgg ttagtaattc tagttggttt agcaatcgtc
    33361 ttatttgcta ttattactac tagtttaatt attgctcgct acaaaccaag ggaaatctta
    33421 tcaaaactgt cctaaagtgc cataagtata ccgaccaata gtgacatcta tattaacggt
    33481 taaaatgaat atgctctgct attgaataca tagctcaaga aagggaaatc tatgttcgaa
    33541 ttaaaaaata ttgcttatcg ttataagggg aacgacaata aaacattaga aaatatcaat
    33601 tatagttttc agtcaggggt attttatact atattaggga attctgattc aggtaaaaca
    33661 acccttctat ccttgatggc aggtcttgat tcgcccactg agggacaagt attatttaac
    33721 aaaaaagata ttaaagaagc aggttatgct cagcatcgca aaaagaatat agctttggtc
    33781 tttcaaaatt ataacctttt agactatcta accccattgg aaaatgttca gttggtaaaa
    33841 cctactgcag ataaacaatt attgttggat ttaggtttaa aagaagatat gttaacgcga
    33901 aatatcttga gattatcagg agggcaacaa caacgtgtag ctattgcgag ggctttggtt
    33961 gcaggaacac cagctatttt acttgatgaa cctacgggga atttagattt tgacattgca
    34021 cgtgatatta cgatgcgtct taaagatttt gcgcataagg aaaaacgatg tgttattatg
    34081 gtgacacata gtagagaaat cgctcatatg gctgatactg ctttacaatt gacaggagat
    34141 aacttaaaag aattatcaaa agaaagtata gcacagtcta tattacaaca aaggaattta
    34201 taataaaaaa atacctccct ttcttaaata ataaaagagg aggtattttt tagaaatggt
    34261 tatttattat cagaaaaggc tcgctctgcc atttgttgaa ttttaattgt agagttattc
    34321 tgataaacga caccattaac cagtgcacca tgcgcatata aacctggcat tggaccatat
    34381 tttggagaca aaacttgtgc attttcagtt aaaatagtaa tccctccttg atcgtcaatt
    34441 tgacaaaatt cttgattgat gagcgatttt aggagtggat tggcttttgt ccagttttta
    34501 gtatttaact ggtaaccagt agcattaatg acgcgatcga cagatatatg attttgtttt
    34561 tctttttcta ttgaaaatcc ttcgtcattt tttgcaattg tcttgacgtt attgatcagg
    34621 gaaaaagtcc ctttttgtgc agctttcaaa attactttag cagattcatc aggcatagga
    34681 tttcgcaagt taataatcgc ttttccatat agtgctttat actctttctt atctgataat
    34741 gacatgagtg gccaaaggtc agttaaccag acagtgattc gtgaagctag ttgttgtaat
    34801 agatagagtt gcttagggta cttaaaggct cgcttgagac catcaattcc tggggataaa
    34861 aaggtttctg ttgtattttt ccagttttta aagccaagat gctccaattc accagacatc
    34921 aaatcattaa aatcggcaat agtgaaggtt tttgaaggat gtgatagaaa tgctttaagg
    34981 ttatcattgg taaaaaagtg ccaagtaagt ggtaaatcat taacaatacg gacgctaggg
    35041 aagtagttgg ttcttgaaaa tgctttaaca gaagattgtg tattgagtag caaccatttt
    35101 ataacatcaa ttgcagctag accagtcccg atgatagcaa ctgtttttcc cttccaattt
    35161 tcctgagcca agtgtttgat aggataaggg tctttgatat aatcagtctt cccttccaaa
    35221 tgataagggt ctataacagg cagagcacca caagctaaat gtaattcatc aaatgtgaca
    35281 ggaaaaagct tatcttgaat atagacttgc cattgttgct tatctgataa atagtttagt
    35341 ttatccactc ttgttttaat aaccgtgaca gggagtttat ctaaaagtaa tttagttctt
    35401 tctgaaaggt attgtccgta aagtgtcctt gatacatagg ttagatttgt gtcatagtaa
    35461 ttttcttcta accaatccat gaagtcagac atatggcgat aatcaaatga aatatcgttg
    35521 attggggaat tgattaaagc tgagtcaacg tcttcttgaa aaggaattcc tcgtccaaag
    35581 tggacagtat catcaaaaca aaaaatagct aactgtttta actctttctc ttttttacga
    35641 gaaagtgcta gtagaacagc taaaccacta actcccatac ctataatacc aattgttttt
    35701 ttcacagaac aagcctcctt accactatca tagcataaat tttgatttaa gttaggggat
    35761 gaacaaagct attatttgca gaatttctaa tttaacgagt aaattaaatt taaagtaata
    35821 ttaaattaag ataagattaa ataaatgatt gacatgctta aataataggc atatgatatg
    35881 gttgtgacaa aaggag
//
LOCUS       DATRNK010000013        35874 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.013, whole genome shotgun sequence.
ACCESSION   DATRNK010000013 DATRNK010000000
VERSION     DATRNK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35874
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.013"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..1306
                     /locus_tag="VBC52_000658"
     CDS             <1..1306
                     /locus_tag="VBC52_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HEO6882259.1"
                     /translation="SLFGVLLLMTPFKYNGMTTVAVSVISKTINQWINAVFPIHYIIL
                     LIIFISCVLALCYRLFRPSFIEKNDLLKEISDITIFWLIIRLIGLALGLMTVLHIGPE
                     MVWGKETGGLILFDLIGGLFTIFLAAGFILPFLTEFGLLEFVGVFLTPIMRPFFQLPG
                     RSAVNCVASFVGDGTIGIALTDKQYVEGYYTSREAATISTTFSAVSITFCLVVLQNVG
                     LTDYFGHFYLTVIISSVVCAFIIPYIPPLSRQSNSYYTKPKKETSEVIPDGYNKIEWG
                     LKLAIDKSKANGNMKTFVNNASKTVLSLWFGVMPTIMTVGTIALIISVSTPIFKILGT
                     PFLPFLELLGIPEADIASQTMIVGFSDMVVPSIMAAEIHSEMTRFIVATVSIVQLIYM
                     SETGAVILGSKIPINILELFIIFIERTIISLPIIVLMAHLFF"
     gene            1490..1624
                     /gene="rpmH"
                     /locus_tag="VBC52_000659"
     CDS             1490..1624
                     /gene="rpmH"
                     /locus_tag="VBC52_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEO6882260.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            1980..2654
                     /locus_tag="VBC52_000660"
     CDS             1980..2654
                     /locus_tag="VBC52_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6882261.1"
                     /translation="MRLLVVEDEKSIAEAIQALLADKGYSVDLVFDGDDGLEYILTGL
                     YDLVLLDIMLPKRSGLSILKRVREAGLETPIIFLTAKSQTYDKVNGLDLGADDYITKP
                     FEADELLARIRLRTRQSSLIRANQLRLGNIRLNTDSHELESKESSVKLSNKEFLLMEV
                     FMRNAKQIIPKNQLISKVWGPSDNSEYNQLEVFISFLRKKLRFLKADIEIITTKGFGY
                     SLEEMT"
     gene            2654..3841
                     /locus_tag="VBC52_000661"
     CDS             2654..3841
                     /locus_tag="VBC52_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6882262.1"
                     /translation="MFSDLRKKFVFLTMSILIVVVLFLFAVSNRYNQYWDEYDAYRIV
                     KLVAKNDYLGIPGDEPIALVTIDNQKMVKIQSNNTDLTNDVIEKSSLKLLEQGKKSRK
                     WKSFIYSIKEYKDKTYTIAIMDLASYEVPYARRFLILVFTIFGFCLLAAVSLYLSRFI
                     VGPVETEMTREKQFVSDASHELKTPIAAIRANVQVLEQQIPGNRYLDHVVSETKRMEF
                     LIEDLLNLSRLDEKRSKVNFKKLNLSVLCQEVLLTYESLAYEEEKCLNDTIEDDVWIV
                     GEESQIKQILIILLDNAIRHSLSKSAIQFSLKQARRKAILTISNPSAIYSKEVMDNLF
                     ERFYQAKDDHADSLSFGLGLSIAKAIVERHKGRIRAYQEKDQLRLEVQLPIDGFWTNT
                     MIN"
     gene            3852..3974
                     /locus_tag="VBC52_000662"
     CDS             3852..3974
                     /locus_tag="VBC52_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEO6882263.1"
                     /translation="MKPYLSFIGRTLLYFGILLLLIYFFAYLGRGQGSFIYNEF"
     gene            3987..5522
                     /gene="dltA"
                     /locus_tag="VBC52_000663"
     CDS             3987..5522
                     /gene="dltA"
                     /locus_tag="VBC52_000663"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEO6882264.1"
                     /translation="MIHDMIKTIEHFAETQADFPVYDILGEVHTYGQLKVDSDSLAAH
                     IDSLGLVEKSPVLVFGGQEYEMLATFVALTKSGHAYIPVDQHSALDRIQAIMTVAQPS
                     LIISIGEFPLEVDNVPILDVSQVSAIFEEKTPYEVTHSVKGDDNYYIIFTSGTTGLPK
                     GVQISHDNLLSFTNWMISDDEFSVPERPQMLAQPPYSFDLSVMYWAPTLAMGGTLFAL
                     PKTVVNDFKKLFATINELPIQVWTSTPSFADMALLSNDFNSETLPQLTHFYFDGEELT
                     VKTAQKLRQRFPKARIVNAYGPTEATVALSAVAITDEMLETCKRLPIGYTKDDSPTYV
                     IDEEGHKLPNGEQGEIIIAGPAVSKGYLNNPEKTAEAFFQFEGLPAYHTGDLGSMTDE
                     GLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSQYVKSAVAVPRYNKDHKVQNLLAYIV
                     LKEGVRDDFERDLDLTKAIKEDLKDIMMDYMMPSKFIYREDLPLTPNGKIDIKGLMSE
                     VNK"
     gene            5519..6784
                     /gene="dltB"
                     /locus_tag="VBC52_000664"
     CDS             5519..6784
                     /gene="dltB"
                     /locus_tag="VBC52_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEO6882265.1"
                     /translation="MIQAFLEKLPHLDVYGNPQYFFYLILAVLPIYIGLFFKKRFALY
                     EIIFSLSFIVMMLTGSTFNQLKSLLAYVVGQSLLVFIYKAYRKRFNHTLVFYVTVCLS
                     IFPLFLVKLIPAISEDGHQSLFGFLGISYLTFRAVAMIIEMRDGVLKEFTLWEFLRFL
                     LFFPTFSSGPIDRFKRFNEDYINIPDRNELLDMLGQAIHYLMLGFLYKFILAYIFGSL
                     IMPPLKELALEQGGVFNWPTLGVMYAFGFDLFFDFAGYTMFALAISNLMGIKSPINFD
                     KPFKSRDLKEFWNRWHMSLSFWFRDFVFMRLVKLLVKNKVFKNRNVTSSVAYIINMLL
                     MGFWHGLTWYYIAYGLFHGIGLVINDAWVRKKKNINKERRLAKKPLLPENKWTYALGV
                     FITFNVVMFSFLIFSGFLDLLWFPQPHNK"
     gene            6799..7038
                     /gene="dltC"
                     /locus_tag="VBC52_000665"
     CDS             6799..7038
                     /gene="dltC"
                     /locus_tag="VBC52_000665"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEO6882266.1"
                     /translation="MDIKSEVLAIIDDLFMEDVSSMMDEDLFDAGVLDSMGTVELIVE
                     LESHFNIDIPIAEFGRNDWNTANKIVAGVTELCNA"
     gene            7031..8293
                     /gene="dltD"
                     /locus_tag="VBC52_000666"
     CDS             7031..8293
                     /gene="dltD"
                     /locus_tag="VBC52_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEO6882267.1"
                     /translation="MLKRLGKVFGPLVCALLLLVGLYLVFPVSQPHHLGKEKNSAVAL
                     TKAGFKSRVQKVRAFSDPKANFVPFFGSSEWLRFDAMHPSVLAEAYNRPYIPYLLGQK
                     GAASLTQYYGIQQIKGQIKNKKAIYVISPQWFVRKGANKGAFQNYFSNDQTIRFLQNQ
                     TGTTYDRYAARRLLKLYPEASMSDLIEKVADGQKLSNKDKQRLKFNDWVFEKTDAIFS
                     YLPLGKTYNQAIMPHVGKLPKAFSYNHLSRIASQDAKVATRSNQFGIDDRFYQTRIKK
                     HLKKLKGSQRHFNYTKSPEFNDLQLVLNEFSKQNTDVLFVIPPVNKKWTDYTGLDQKM
                     YQKSVEKIKHQLQSQGFNHIADLSRDGGKPYFMQDTIHLGWNGWLELDKHINPFLTEE
                     NSKPNYHINNKFLKKSWAKYTGRPSDYK"
     gene            8468..8860
                     /locus_tag="VBC52_000667"
     CDS             8468..8860
                     /locus_tag="VBC52_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 domain-containing protein"
                     /protein_id="HEO6882268.1"
                     /translation="MKSIKLLSFIVIILLGLGFTVGGLKVTENNKQQELWEIANSKDA
                     KNAYQKWIYAEDKDAFKENAVIKSYDIDKESIKKNPMGGISVRLIINKDPNLYITCNL
                     DKDNQGHLVSQSSHQSPQLTHLLESRGH"
     gene            8860..10152
                     /locus_tag="VBC52_000668"
     CDS             8860..10152
                     /locus_tag="VBC52_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6882269.1"
                     /translation="MEKVLTDLDRVNIAKQEYELGSQLDTLVKIMSQDKVLPIGKVAH
                     VQDGGKETGEQIYTITPNGTLDKPEDVKEVTVLFKGSTAPFGGDDWKTDWFKNDIPIA
                     SKLLLKKFGSQSVSHKQGTKQLEQSAHLLKEVMNKYPNAKISVYGHSLGSMNAQYAIA
                     ELDDNQIYRVQGAWIYNGPNLYSILTDKQKEQVDKIKGVVNNYVDSKDKIGIGYPKEG
                     SDNAVGIVKIVDSKAVSGGSWVANFTNQHIWGGYQYNQDGSLKLAEDKFAYKENYSVV
                     HTNVNQRMHAIKAKLSSFKSTGKEVSKNQAIFLDSQQAKVISSGLVTISKEAVEEVKK
                     VEQKAVAKAEELYQSLKVAPTGVTELSPQELEEAYAQSGVSYASIVGEAQTFFSNKIQ
                     AAESISHAYVELENQINKGVEAMLQQDSNLSGEFKQWH"
     gene            10143..10535
                     /locus_tag="VBC52_000669"
     CDS             10143..10535
                     /locus_tag="VBC52_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882270.1"
                     /translation="MALIDIIEKQLADTQRKISDLDDAYHHSCCQFEEKLDDLSVRKN
                     KITNMLQETYDAVEYDLRYSNDSSDMMTLNRILDSYHDDLEQAYHKEYYALSAQEEEY
                     RANYIRQRSEHELTFEELQREKKRELMK"
     gene            10545..10826
                     /locus_tag="VBC52_000670"
     CDS             10545..10826
                     /locus_tag="VBC52_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882271.1"
                     /translation="MSLMWDVLTGKESVRTLKKQLDKELIHSVYAPRKRSKFHDIITS
                     HHKEIDEVAINTKTAIHKVANQLDEAIKGEFRSRVEEAIESNQQKYNHL"
     gene            11008..11790
                     /locus_tag="VBC52_000671"
     CDS             11008..11790
                     /locus_tag="VBC52_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEO6882272.1"
                     /translation="MIKIFDTHTHLNVENFEGKIDEEINLASELGVIKMNVVGFDQDT
                     ISKSLELSSQYAQVYSTIGWHPTEAGSYDDNIESMIISHLENPKVIALGEIGLDYYWM
                     EDPKDIQIEVFKRQIELSKEYNLPFVVHTRDALEDTYEVIKESGVGPFGGIMHSFSGS
                     LEMAQKFIDLGMMISFSGVVTFKKALDVQEAARELPLDKILVETDAPYLAPVPKRGRE
                     NKTAYTRYVVEKIAELRGITVEEVAEATYQNAVRIFRLDEKN"
     gene            11777..12343
                     /gene="rnmV"
                     /locus_tag="VBC52_000672"
     CDS             11777..12343
                     /gene="rnmV"
                     /locus_tag="VBC52_000672"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEO6882273.1"
                     /translation="MKKIDIQEVIVVEGKDDTANLRRFYNVDTYETRGSAIDEEDLER
                     IERLHNLRGVIVFTDPDYNGERIRKIIMNAIPTVRHAFLNRDEAKPGSKTKGRSLGVE
                     HASFEDLQKALSKVTQHFDDEDNFDITQADLIRWGFITASDSRKRREYLGNQLRIGYS
                     NGKQLLKRLRLFGVTKAEVEECMEGYEG"
     gene            12347..12796
                     /locus_tag="VBC52_000673"
     CDS             12347..12796
                     /locus_tag="VBC52_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEO6882274.1"
                     /translation="MYLICQRINMIEQNENPYFVKEYETGYLVLGDYQYFQGYCLFLS
                     KKHVTELHELPRDWRNQYLSEMADASEIVAKAFRADKMNIESLGNGDAHLHFHLFPRK
                     TGDLRNYGHNGKGPVWWYPFEKMYADSVRATGAEIEKLKEKLLDVLG"
     gene            12824..13696
                     /gene="rsmA"
                     /locus_tag="VBC52_000674"
     CDS             12824..13696
                     /gene="rsmA"
                     /locus_tag="VBC52_000674"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEO6882275.1"
                     /translation="MRIADKTVTRAILERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DKGVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLIPILAGTLARFDNVQVVNQDIL
                     KADLQTQIQAFKNPDLPIKVVANLPYYITTPILMHLIESKIPFAEFVVMMQKEVADRI
                     SAMPNTKAYGSLSIAVQYYMTAKVSFIVPRTVFVPAPNVDSVILKMVRRDQPVVSVQD
                     EDFFFRVSKVAFVHRRKTLWNNLTSHFGKSEDTKAKLEKALEIAKIKPSIRGEALSIP
                     DFASLADALKEVEI"
     gene            13700..14551
                     /locus_tag="VBC52_000675"
     CDS             13700..14551
                     /locus_tag="VBC52_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO6882276.1"
                     /translation="MLVDKKWRFEDSASYFACPKCQNPLIKESNSLKCSGNHCFDLSK
                     FGYVNLLGGKKVDEHYDKKSFENRQLVLENGYYNHILEAISKVLENNSQFHSVLDIGC
                     GEGFYSRQLVNKHEKTFLAFDISKDSIQLAAKSDQSRLVKWFVSDLANLPIQDSSIDI
                     ILDIFSPANYKEFRRVLSDDGILVKVVPVAEHVQELREKASQYLKQKDYSNQKILDHF
                     RENFEIISEQKVVQSYNCSQQERQAFIDMTPLLFSVDKTTIDWASISEITVGALIVIG
                     KKRSVSK"
     gene            14707..15579
                     /gene="rsgA"
                     /locus_tag="VBC52_000676"
     CDS             14707..15579
                     /gene="rsgA"
                     /locus_tag="VBC52_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEO6882277.1"
                     /translation="MQGRIVKSLAGFYYVESDGVVYQTRARGNFRKKGQIPYVGDWVE
                     FSSQDQSEGYILSIEERKNSLVRPPIVNIDQAVVIMSAKEPDFNANLLDRFLVLLEYK
                     MIQPIIYISKLDLLDDLVVIDDIREHYQNIGYVFCYSQEELLPLLADKVTVFMGQTGV
                     GKSTLLNKIAPELKLETGEISDSLGRGRHTTRAVSFYNVHKGKIADTPGFSSLDYEVD
                     NAEDLNESFPELRRLSHFCKFRSCTHTHEPKCAVKEALTQGQLWQVRYDNYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            15586..16248
                     /locus_tag="VBC52_000677"
     CDS             15586..16248
                     /locus_tag="VBC52_000677"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEO6882278.1"
                     /translation="MSTNKIAPSILAADYANFANELKRIEETTAEYVHIDIMDGQFVP
                     NISFGADVVSSMRKHSKLVFDCHLMVVDPERYIEAFAQAGTDIMTIHVEATKHIHGAL
                     QKIKEAGMKAGVVINPGTPVESLIPILDLVDQILIMTVNPGFGGQAFIPEMMSKVKTV
                     AAWRKEYGHHYDIEVDGGIDNTTIKAAAEAGANVFVAGSYLFKASDLPAQVETLRVAL
                     DD"
     gene            16241..16873
                     /locus_tag="VBC52_000678"
     CDS             16241..16873
                     /locus_tag="VBC52_000678"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEO6882279.1"
                     /translation="MTKIALFAGGDLTYFEYDFDYFVGIDRGSLFLLKNGLSLDMAVG
                     DFDSITEDELLYIKHYCSNIVSASVEKNDTDTELALKTIFKEFPEAQVTVFGAFGGRI
                     DHMMSNIFLPSDTDLEPFMSQIRLKDEQNIVTYLPSGKNQVSRIEGMSYVSFMPESES
                     TLQISGAKYELNKSNYFKKKMYSSNEFMTSPIEVELKDGYLIIIYSKDRG"
     gene            16875..18149
                     /locus_tag="VBC52_000679"
     CDS             16875..18149
                     /locus_tag="VBC52_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEO6882280.1"
                     /translation="MLVIILIIALASLTVTIISYQKMTELTKSVEKQLEDNADNLSDQ
                     LTYQIEAAQKDQILTLTNQLNRMQQEIYQLLTDMRTELNQHLTESRDRSDKRLELINS
                     NLSQSVQKMQDSNEKRLDQMRQTVEEKLEKTLQTRLQTSFETVSRQLESVNQGLGEMK
                     TVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTVSQYEREFPTVSGSSERVEYAI
                     KLPGNGQGDYIYLPIDSKFPLEDYYRLEDAYELGDKVQIELYRKSLLASIRKFAKDIN
                     NKYLNPPETTNFGIMFLPTEGLYSEVVRNATFFDSLRRDENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNANDISKILGNVKVEFGKFGGMLSKAQKQLNTASKSIDSLLTTRTNAI
                     IRALNTVEEHQDQATTSLLNLPITEEEEINEN"
     gene            18139..19080
                     /locus_tag="VBC52_000680"
     CDS             18139..19080
                     /locus_tag="VBC52_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEO6882281.1"
                     /translation="MKINQMKKDELFEGFYLIKKAEVRKTRAGKDFIAFTFQDDTGEI
                     SGNMWDAQTYNVEEFVAGKIVHMKGRREVYNGTPQVNQITLRNIKDGEPNDPRDFKEK
                     PPINVDNVREYMEQMLFKIENATWQRVVRALYRKYNKEFFTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYLELNKSLMFAGIMLHDLAKVIELSGPDNTEYTIRGNLIGHIS
                     LIDEELTKILAELNIDDTKEEVTVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALNRVNEGEMTNRIFAMDNRSFYKPNIK"
     gene            19177..19989
                     /gene="purR"
                     /locus_tag="VBC52_000681"
     CDS             19177..19989
                     /gene="purR"
                     /locus_tag="VBC52_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEO6882282.1"
                     /translation="MKLRRSERMVVISNYLINNPYTLTSLNTFASKYGAAKSSISEDI
                     AIIKKAFEQAQIGDIKTVTGASGGVIFTPTIAEAEAKEIVEELRQRLSENDRILPGGY
                     IYLSDLLSTPKMLQSIGRIIANAYRGQKIDAVMTVATKGVPLANAVANVLDVPFVIVR
                     RDLKITEGSTVSVNYASGSSGRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTVSGMISL
                     LSEFDSTLVGVAVFAENAQEQREKMAYKSLLRVSEIDVKNNRVSVEAGNIFD"
     gene            20208..20621
                     /gene="rpsL"
                     /locus_tag="VBC52_000682"
     CDS             20208..20621
                     /gene="rpsL"
                     /locus_tag="VBC52_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEO6882283.1"
                     /translation="MPTINQLVRKPRKSKVEKSDSPALNIGYNSHRKVHTKLSAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            20643..21113
                     /gene="rpsG"
                     /locus_tag="VBC52_000683"
     CDS             20643..21113
                     /gene="rpsG"
                     /locus_tag="VBC52_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEO6882284.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFEAIKEATGTDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            21268..23346
                     /gene="fusA"
                     /locus_tag="VBC52_000684"
     CDS             21268..23346
                     /gene="fusA"
                     /locus_tag="VBC52_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEO6882285.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQSLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYVDQANEYREKLVEAV
                     ADTDEDLMMKYLEGEEITNEELMAAIRKATINVEFYPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDEEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVLNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     SKVILESIEVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEARGNTQVVRAFVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNSGE"
     gene            23551..24561
                     /gene="gap"
                     /locus_tag="VBC52_000685"
     CDS             23551..24561
                     /gene="gap"
                     /locus_tag="VBC52_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEO6882286.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGQFVKVSAEREPANIDWATDGVEIVLEATGFFAS
                     KEKAEQHIHENGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALQDNFGVKQGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVATLEKDVTVEEVNAAMKAAANDSYGYTED
                     PIVSSDIVGISYGSLFDATQTKVQTVDGNQLVKVVSWYDNEMSYTSQLVRTLEYFAKI
                     AK"
     gene            24770..25639
                     /locus_tag="VBC52_000686"
     CDS             24770..25639
                     /locus_tag="VBC52_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEO6882287.1"
                     /translation="MKTTKVFSVLSLSVAVFMMTACQSNHSTKKADMSPDKVEQKTKF
                     KNISLSSNKLLAKENTMSVLWYQNSAEAKALYLQGYNVAKMKLDDWLQKPSEKPYSII
                     LDLDETVLDNSPYQAKNIKDGSSFTPESWDKWVQKKSAKAVAGAKEFLKYANEKGIKI
                     YYVSDRTDAQVDATKENLEKEGIPVQGKDHLLFLKKGMKSKESRRQAVQKDTNLIMLF
                     GDNLVDFADFSKSSSTDREQLLTKLQSEFGSKFIVFPNPMYGSWESAIYQGKHLDVQK
                     QLKERQKMLHSYD"
     gene            25774..26970
                     /locus_tag="VBC52_000687"
     CDS             25774..26970
                     /locus_tag="VBC52_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEO6882288.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGAKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            27233..27772
                     /locus_tag="VBC52_000688"
     CDS             27233..27772
                     /locus_tag="VBC52_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEO6882289.1"
                     /translation="MKTLKKLLSNYKFDIKKFKLGMRTFKTGLSVFLVLLVFHLFGWK
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMINVACNNKSGIIGGTAALLIITLSIPSGETILYVFARIFETFCGVFIA
                     MMVNTDIEILRKKLKNNKL"
     gene            27852..28223
                     /locus_tag="VBC52_000689"
     CDS             27852..28223
                     /locus_tag="VBC52_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6882290.1"
                     /translation="MKERELRRTMAVFPIGAVMKLTDLTARQIRYYEDQGLITPERTE
                     GNRRMFSLNDMDRLLEIKDFISDGLHISDIKNEYMQRQHKSKEKQKSLSDAEVRRLLQ
                     DELRNQGRFSSPSQHIGNMHL"
     gene            28257..29603
                     /gene="glnA"
                     /locus_tag="VBC52_000690"
     CDS             28257..29603
                     /gene="glnA"
                     /locus_tag="VBC52_000690"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEO6882291.1"
                     /translation="MTITAEDIRREVKEKNVTFLRLMFTDILGVMKNVEIPATDEQLD
                     KVLSNKAMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     EPFAGDPRGNLKRNMKRMQEMGYKSFNLGPEPEFFLFKMDENGNPTLDVNDKGGYFDL
                     APTDLADNTRREIVNVLTQMGFEVEASHHEVAVGQHEIDFKYDDVLKACDNIQLFKLV
                     VKTIARKHGLYATFMAKPKFGINGSGMHCNMSLFDNEGNNAFFDPEDPRGMQLSEDAY
                     YFLGGLMKHAYNYTAIINPTVNSYKRLVPGYEAPVYIAWAGRNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALSVLLGSGLEGIENKIEAPEPIETNIYAMTVEERRQAGIVDL
                     PSTLHNALKALEEDEVVKAALGTHIYTNFLDAKRIEWASYATYVSQWEIDNYLDLY"
     gene            29751..30260
                     /locus_tag="VBC52_000691"
     CDS             29751..30260
                     /locus_tag="VBC52_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6882292.1"
                     /translation="MEENMNIKQLKSKTMLGTVALVSAFSFASTNADANTYNYAVDVD
                     YLASAEEIAQAHPASNTFPLGQCTWGVKEMATWAGNWWGNGGDWAASAASAGYTVGTQ
                     PRVGSIVCWTDGSYGHVAYVTAVDPVTNKIQVLESNYAGHQWIDNYRGWFDPQNTVTP
                     GVVSYIYPN"
     gene            complement(30422..32101)
                     /locus_tag="VBC52_000692"
     CDS             complement(30422..32101)
                     /locus_tag="VBC52_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO6882293.1"
                     /translation="MSNINLKPEEVGVYAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVENIDRIKALVITHGHEDHIGGIPFLLKQANLPIYAGPLA
                     LALIKGKLEEHGLLRDATLYEIHANTELTFKNLSVTFFRTTHSIPEPLGIVIHTPQGK
                     VICTGDFKFDFTPVGEPADLHRMAALGEDGVLCLLSDSTNAEVPTFTNSEKIVGQSIM
                     KIIEGIEGRIIFASFASNIFRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKHLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKINNIHTSGHGGQQEQKLMLRLIKPKYFMPVH
                     GEYRMQKVHAGLAVDTGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRHDLSEDGVVLAVATVDFDSKMILAGPDILSRGFIYMRESGDLIRESQH
                     ILFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDK"
     gene            complement(32155..32385)
                     /locus_tag="VBC52_000693"
     CDS             complement(32155..32385)
                     /locus_tag="VBC52_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEO6882294.1"
                     /translation="MIYKVFYQETKERNPRREQTKTLYITIDAANELEGRIAARKLVE
                     ENTAYNIEFIELLSDKHLEYEKETGVFELTEF"
     gene            32564..33256
                     /gene="tsaB"
                     /locus_tag="VBC52_000694"
     CDS             32564..33256
                     /gene="tsaB"
                     /locus_tag="VBC52_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEO6882295.1"
                     /translation="MMKVLAFDTSSKALSVAVLNNMECLATVTINIKKNHSINLMPAI
                     DFLMQSIDLEPQDLDRIVVAEGPGSYTGLRVAVATAKMLAYTLKIDLVGVSSLYALTN
                     GFSENDLLVPLIDARRNNVYVGFYQNGDTVKPDCHTSLEEVLQEVGNKANVHFVGEVA
                     AFVDQIKKVLPHAKITETLPCAVAIGRKGQKMKSVNVDAFVPRYLKRVEAEENWLRNH
                     CETNTEEYIKRV"
     gene            33258..33665
                     /gene="rimI"
                     /locus_tag="VBC52_000695"
     CDS             33258..33665
                     /gene="rimI"
                     /locus_tag="VBC52_000695"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEO6882296.1"
                     /translation="MESSEQALAIWSVLSDVYDKSPWSLSQISEDLKKDSTDYFFVYN
                     DGEVIGFLALQQLVGEVEITNIAVKKNYQGKGYAYQLMSMIADIEVPVFLEVRYSNIV
                     AQKLYERCGFVVLGKRKNYYHDPIEDAIVMRKG"
     gene            33741..34751
                     /gene="tsaD"
                     /locus_tag="VBC52_000696"
     CDS             33741..34751
                     /gene="tsaD"
                     /locus_tag="VBC52_000696"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEO6882297.1"
                     /translation="MKDRYILAVESSCDETSVAILKNDKELLANIIASQVESHKRFGG
                     VVPEVASRHHVEVVTTCFEDALQEAGIVASDLDAVAVTYGPGLVGALLVGMAAAKAFA
                     WANKLPLIPVNHMAGHLMAARDVKELQYPLLALLVSGGHTELVYVSEPGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMIKEDHLEFSFSGLKSA
                     FINLHHNAEQKGEALVLEDLCASFQAAVLDILLAKTQKALLKHPVKTLVVAGGVAANQ
                     GLRERLATDISPDIDVVIPPLRLCGDNAGMIALAAAIEFEKENFASLKLNAKPSLAFE
                     SL"
     gene            34870..>35874
                     /locus_tag="VBC52_000697"
     CDS             34870..>35874
                     /locus_tag="VBC52_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEO6882298.1"
                     /translation="MLHFENDYNKGAHPELLNALIETNDQGLSGYRTDSYCQQAADKI
                     REVCFCPQAEVEFLVGGTQTNQVVISSMLASYEGVIAAETGHVSSHEAGAIEFSGHKV
                     LTLPSHNGKLLASEVATYIETFYADGNYQHMVFPGMVYISHPTEYGTLYSKAELEELS
                     KICKHYQIPLFIDGARLGYGLAAKDTDVDFPTIAALSDVFYIGGTKMGALAGEAVVFT
                     KKNRPKQFTTIVKQHGALLAKGRLLGLAFDRFFTDDLYLKIGKHAIDLAEELKIILEE
                     KGYSFYLKSPTNQQFIIVENTKLADLAKNVAYSFWEKYDDHHTVIRLATSWSTSREDV
                     T"
BASE COUNT        11711 a         5741 c         7258 g        11164 t
ORIGIN      
        1 gtcgttattt ggagtattat tattaatgac tccatttaaa tataatggta tgacaacagt
       61 agctgtctct gtaatatcta agacaattaa tcaatggatt aacgctgttt ttcctataca
      121 ttatattatt ttactaatca tttttatttc atgtgtgtta gcactttgtt atcgtctgtt
      181 tagacctagc tttattgaaa aaaatgactt gttaaaagaa atttctgaca ttaccatttt
      241 ctggctgatt attcgtttga tcggtttggc acttggtctg atgacggttt tacatattgg
      301 tcctgaaatg gtatggggga aagagacagg cggccttatc ctttttgatt tgattggtgg
      361 tttgtttacc attttccttg ctgcgggttt tattcttcca tttttgacag aatttggtct
      421 actggagttt gttggtgtct tcttaacccc cattatgcgt ccattctttc agctacctgg
      481 tcggtcagca gttaattgtg tagcctcatt tgtaggtgat ggcactattg gaattgcttt
      541 gaccgataag cagtatgttg agggttatta taccagtaga gaagctgcaa cgatttccac
      601 tactttttca gcagtatcaa tcactttttg tcttgttgtt ttgcagaatg ttggcttaac
      661 tgattatttt ggccattttt atctaacagt catcattagc agtgttgtct gtgcattcat
      721 tatcccttat atccctccct tatctcgtca atcaaatagt tactacacta aaccaaagaa
      781 agaaacatct gaggtaattc cggatggtta taacaagata gagtggggac ttaagcttgc
      841 catcgataaa tcaaaagcaa atggaaacat gaagactttt gtcaataatg cctctaagac
      901 agtacttagt ttatggtttg gcgttatgcc aacaattatg actgtcggaa caatagcttt
      961 gattatttca gtatcaacac ctatttttaa aattttaggc acaccattcc ttccgttcct
     1021 tgaactttta gggattccag aagcagatat tgcgagtcaa accatgattg ttggtttttc
     1081 agatatggtt gttccttcga taatggcggc tgaaattcac agtgagatga caaggtttat
     1141 tgtcgcaaca gtttctattg tgcaattaat ttatatgtct gagactggtg cggtgatatt
     1201 aggttccaag atccctatca acattctaga attatttatc atttttatag agagaacaat
     1261 tatttcatta cctattattg ttttgatggc acatcttttc ttttagttgg agaaaataga
     1321 gaagcatttc ttgctaacaa atggaactat ttactgaaaa aagacaaatg aacttgaaag
     1381 tatttcttga caagaggcct aaaattgtta taaaatagta tggtatgttt agtaactgat
     1441 caaaatagcc ggctaaacga atacgaaaat ctatcaggag gtattcaacg tgaaacgtac
     1501 ttatcaacca agtaaaatcc gtcgccaacg taaacacgga ttccgtcacc gtatgtcaac
     1561 taaaaacgga cgtcgcgtgc ttgctagccg ccgtcgtaaa ggacgtaaag tgttatcagc
     1621 ttaatataga tagcaaaata atatcaagcc aacaggtact cgagtctggt ggcttttttg
     1681 gtaccttcat aacgataaag gtttttgtga gagtagtttg agtatattat agataaagtt
     1741 aatgaaaagt gagtttataa tgcggaaatt cgtagacaaa tagctcctat ttttgttaaa
     1801 atatgaggag atttcttatt gagaaatatc tttatagtga gtgatatttt attttttaga
     1861 cgcttgtatt attgaagtag tttagtgact taggaaatac ttttattaat atcgtttttt
     1921 caaggttatt tcaattttgc tagtttatac tataatatat aaggtaaggt gactagaaaa
     1981 tgagacttct tgtagttgag gatgaaaaaa gtatagcaga ggctattcag gctttattgg
     2041 cagataaagg ttattctgtt gatttagtat ttgatggaga tgatgggtta gaatacattt
     2101 tgacaggtct ctatgattta gtcttgttgg atattatgtt acccaaacgc tcaggattat
     2161 ctattttaaa aagagtgagg gaagctggtt tagaaacccc aataatcttt ttaactgcaa
     2221 aatcacagac ttatgataaa gttaatggtt tagatttagg ggcagatgat tatatcacaa
     2281 aaccctttga agcagatgaa ttgctagcta gaattaggtt gagaaccaga caatcttctc
     2341 tgatcagagc aaatcaattg cggcttggaa atataaggct taacacagat tcacatgagc
     2401 tagaatctaa agaaagtagt gtaaaattat ccaataaaga atttctcttg atggaagttt
     2461 ttatgcgaaa tgcaaagcag attattccta aaaaccagtt gatttctaag gtttggggac
     2521 cttcagacaa ttcagaatat aaccagttag aagtatttat ttcttttttg cgaaaaaaat
     2581 tgcgcttttt aaaagcagac attgaaataa taaccactaa gggctttgga tattccttgg
     2641 aggagatgac ataatgttta gtgatttacg caaaaaattt gtctttttga ccatgtcaat
     2701 ccttattgtc gttgttctct ttctctttgc agtgagtaat cgctacaatc aatattggga
     2761 tgaatatgat gcatatagga ttgttaaatt agttgctaaa aatgattatt taggaatacc
     2821 aggcgatgaa ccgattgctt tggtgactat agataaccaa aagatggtta agattcaaag
     2881 caataatact gatttgacaa atgatgttat cgaaaaatct agtttaaagc tccttgagca
     2941 aggaaagaag tcacgaaaat ggaagtcttt tatatattcc ataaaagaat ataaagataa
     3001 aacttacacc atagctatta tggatttggc tagttatgaa gtcccttatg caaggcgttt
     3061 tctcattctt gtttttacta tttttggatt ttgtttatta gcagctgtaa gtctttatct
     3121 ttctcgattt atcgtagggc ctgtagagac agaaatgact cgcgaaaaac agttcgtttc
     3181 agatgcgagt catgagctga agacccctat tgcagctata cgagccaacg ttcaggttct
     3241 ggaacagcaa attccaggaa atcgttatct ggatcatgtt gtttcggaga ctaagcgaat
     3301 ggagttttta attgaagatt tgcttaattt gtctagactt gatgaaaaac gctccaaggt
     3361 aaatttcaaa aagttaaact tatctgtctt atgtcaagaa gtacttctaa catatgaaag
     3421 tttggcatat gaagaagaaa aatgtttaaa tgatacaatt gaagatgatg tctggattgt
     3481 tggagaagag agtcaaatta agcaaatttt aataattctt ttggataatg ctattaggca
     3541 ttctttgtca aaatcagcaa tccagtttag tcttaaacag gctcgaagaa aagctattct
     3601 gacaatatca aatccaagtg ctatctattc caaagaggtt atggataatc tctttgagcg
     3661 tttttatcaa gctaaagatg atcatgcaga ttctctcagt tttggtctag gcttatctat
     3721 tgctaaggca atcgtggaac gccataaggg gagaattaga gcatatcaag aaaaagacca
     3781 attacgtctt gaagtccagt taccaattga tggattttgg accaatacca tgattaatta
     3841 aaggaagaat gatgaaacca tatttatctt ttattggtag aacgttatta tacttcggta
     3901 ttttattgtt actaatttac ttttttgcat accttggtcg cggacaaggc agttttattt
     3961 ataatgaatt ctaggagatg gtacaaatga tacatgatat gattaaaaca attgagcatt
     4021 ttgctgagac acaagctgat tttccagtgt atgatatttt aggggaagtc catacttatg
     4081 gacaacttaa agtagactct gactctctag ctgctcatat tgatagccta ggccttgttg
     4141 aaaaatcacc tgtcttagta ttcggtggtc aagaatatga aatgttggcg acatttgttg
     4201 ctttaacaaa gtcagggcat gcttatatac cggttgacca acactctgct ttggatagaa
     4261 tacaggctat tatgacagtt gctcaaccaa gccttatcat ttcaattggt gaatttcctc
     4321 ttgaagttga taatgtccca atcctagacg tttctcaagt ttcagctatt tttgaagaaa
     4381 agactcctta tgaggtaaca cattctgtta aaggtgatga taattactat attattttca
     4441 cttcagggac tactggttta ccaaaaggtg tgcaaatttc acatgacaat ttattgagct
     4501 ttacaaattg gatgatttct gatgatgagt tttcagttcc tgaaagaccg caaatgttgg
     4561 ctcaaccgcc atattcattt gacttatcag ttatgtattg ggcaccaaca ctagctatgg
     4621 gaggcaccct gtttgcccta ccaaaaacag tagttaatga tttcaaaaaa ctattcgcta
     4681 ccattaatga attgccaata caggtttgga cttcgacacc atcatttgct gatatggcgc
     4741 tactatctaa cgatttcaat tcagagacct tgccacagtt aacacatttt tattttgatg
     4801 gggaagagtt aactgtcaag actgcacaaa aacttcgtca acgttttcca aaagctcgta
     4861 tcgttaatgc atatgggcca acagaagcaa cagttgctct atccgcagta gcaattactg
     4921 atgaaatgtt agaaacatgc aaacgccttc caattggtta cactaaagat gactcaccaa
     4981 cgtatgtgat tgatgaagaa ggtcataaat taccaaacgg agagcaaggt gaaatcatta
     5041 ttgctggacc agcagtatca aaaggctatc ttaacaatcc agaaaagaca gctgaggcat
     5101 ttttccaatt cgaaggtcta cctgcttacc ataccggtga cttaggaagt atgaccgatg
     5161 aaggtcttct gctttacggt gggcgtatgg atttccaaat taaatttaac ggctatcgta
     5221 ttgaattaga agatgtttct caaaacttaa acaaatcgca gtatgtaaaa tcagcagtag
     5281 cagtgccacg ttataacaag gatcataaag ttcaaaactt attagcctat attgtcttaa
     5341 aagaaggtgt aagagatgat tttgaacgtg atttggattt gacaaaagca attaaggaag
     5401 acttaaagga cattatgatg gattacatga tgccatctaa atttatctat cgagaagatt
     5461 tacctttgac accaaatggg aaaattgata tcaaaggtct tatgagcgag gtaaacaagt
     5521 gatacaggca tttttagaaa aacttcctca cttggatgtt tatgggaatc cacaatattt
     5581 cttctacctt atcttagctg ttctaccaat ttacatcggc ttattcttta agaagcgttt
     5641 tgccttatat gagattattt ttagtctaag ttttattgta atgatgttga ctggtagtac
     5701 ttttaatcaa ttgaagtcac tattggcata cgttgtcgga caatctctgc tagtttttat
     5761 ctataaagct taccggaaac gatttaatca tactttggtc ttttatgtaa cggtttgttt
     5821 atctattttt ccgctatttt tggtaaaatt aattccagct atatctgagg atgggcatca
     5881 gtcacttttt gggtttctag gaatttctta ccttactttt agagctgttg ctatgattat
     5941 tgaaatgaga gacggtgttt tgaaagaatt tactttatgg gaattcttaa gatttttact
     6001 cttctttcca actttctcaa gtggaccaat tgatcgtttt aaacgattca atgaggatta
     6061 cattaatatc ccagatcgaa acgaactcct agatatgtta ggtcaagcga ttcattattt
     6121 gatgttaggt tttctctata agtttatttt agcctatatt tttggaagtc tgattatgcc
     6181 tcctctaaaa gaattagcgc tagaacaggg tggtgtgttt aattggccaa cacttggggt
     6241 tatgtatgcc tttggttttg atttgttctt tgattttgca ggttacacaa tgtttgcgtt
     6301 ggctatttct aacctaatgg ggattaagtc tccgattaac tttgacaaac ctttcaaatc
     6361 acgcgaccta aaagaatttt ggaatagatg gcatatgagc ctttctttct ggtttagaga
     6421 ctttgttttc atgaggcttg ttaagctttt agttaaaaat aaagttttta aaaaccgtaa
     6481 tgttacttca agtgtagctt atattatcaa tatgcttctt atgggattct ggcatgggtt
     6541 aacttggtac tatatagcct atggtctctt tcatgggatt ggcctagtta ttaatgacgc
     6601 ttgggtacgt aagaagaaaa atattaataa agaaagaaga ttggctaaaa aaccactttt
     6661 accagaaaac aaatggactt atgctttggg tgtcttcatc acctttaatg tagttatgtt
     6721 ttctttcttg attttttcag gatttttaga tcttttgtgg ttcccacaac cgcataacaa
     6781 ataataagga gtattataat ggatattaaa tcagaagtat tagcaattat tgatgaccta
     6841 tttatggagg atgtttcttc tatgatggat gaagacttat ttgacgcagg agttctagac
     6901 agcatgggta ctgttgaact aattgttgag ttggaaagcc attttaatat tgatattccg
     6961 attgcggaat ttggtcgtaa tgactggaac acagctaata aaattgttgc tggagtaacg
     7021 gagctctgca atgcttaaac gtctaggtaa agtatttgga cctctagttt gtgctttact
     7081 attgttggta ggattatatc ttgtttttcc tgtttctcag cctcatcatt taggtaagga
     7141 aaaaaacagt gcagtagcgt tgacgaaggc aggttttaaa agcagagttc aaaaagttag
     7201 agctttcagt gatcctaaag ccaattttgt ccctttcttt ggttcaagtg agtggctaag
     7261 atttgatgca atgcatccat cagttttagc agaggcttac aatagacctt atatcccata
     7321 tcttttaggt caaaaagggg cggcttctct gacacaatac tatggcattc aacagattaa
     7381 aggacaaatc aaaaataaaa aagctatcta tgttatttct ccgcaatggt ttgttcgcaa
     7441 gggagccaac aaaggtgctt ttcaaaacta tttcagcaac gatcaaacca ttcgattttt
     7501 gcaaaatcaa acagggacaa cctacgatag gtatgctgct cgtcgattgt taaaattata
     7561 tcctgaagct tctatgtcag atttgataga aaaagttgca gatggccaaa aactatcaaa
     7621 taaagacaaa caaagactaa agtttaatga ttgggtattt gagaagacag atgctatttt
     7681 tagctatcta ccactaggaa aaacttataa tcaggcaata atgcctcatg ttggtaaatt
     7741 accgaaagca ttctcatata atcatttatc gcgtattgca tcacaagatg ctaaagtagc
     7801 aacgagatca aatcaatttg gtattgatga tcgcttttac caaacaagaa ttaaaaagca
     7861 cttaaaaaaa ttgaaagggt cacaacgaca tttcaattat actaagtcac cagaatttaa
     7921 tgatttacag ttggttctta atgaattctc aaaacaaaat acagatgtcc tttttgtcat
     7981 accaccagta aataaaaagt ggacagacta cacagggctt gatcaaaaaa tgtatcaaaa
     8041 atctgtagaa aaaataaaac accaacttca aagtcaaggt ttcaatcata tcgctgacct
     8101 ttctcgagat ggaggtaagc catactttat gcaagataca atccatttag gttggaatgg
     8161 ttggttagag ctagataagc atatcaatcc atttttaaca gaggaaaaca gcaagccgaa
     8221 ttatcacatt aataataaat ttttgaagaa atcttgggca aaatatacag gacgtccaag
     8281 tgattacaag taaatagtag agtcagatga tttatagcat tagagctatt aatcatcttt
     8341 tttaatatct ttgtatttgg taatcctaag ataactaatc catgttttat agtttttcgt
     8401 ttataatgag aataaattta tgataaacta gatatcaaat gagtgttata tgataaaagg
     8461 aggagagatg aagtcaataa aattacttag ttttattgta ataatccttt tagggcttgg
     8521 tttcactgta ggaggtttaa aagtgactga aaacaataaa caacaagaat tatgggaaat
     8581 agctaatagc aaggacgcta aaaatgctta tcaaaagtgg atttatgcag aagataaaga
     8641 cgctttcaaa gagaatgctg ttattaagtc gtatgatatt gacaaagaaa gtatcaaaaa
     8701 aaatcctatg ggagggattt cggtaaggtt aatcattaat aaagatccta acttatatat
     8761 tacttgtaat ttggataagg ataatcaagg gcatttggtc agtcaaagta gccatcaatc
     8821 tccccagtta actcatttat tggaaagtag aggtcattaa tggaaaaagt cttgactgat
     8881 ttagataggg taaatatagc gaagcaagag tatgaattag gtagccaact tgatacacta
     8941 gtcaaaataa tgagccaaga taaggtactc cccatcggta aagttgctca cgttcaagat
     9001 ggaggcaaag aaactgggga acaaatttat accatcactc caaatggaac tttagacaag
     9061 ccagaggatg ttaaggaagt tacggtttta tttaaggggt caacagcacc gtttggagga
     9121 gatgattgga aaacagactg gtttaaaaat gatattccaa tagctagtaa actattatta
     9181 aagaaatttg gttcacaaag tgtatctcat aaacaaggta caaagcagtt agaacaatca
     9241 gctcatctgc taaaagaagt tatgaataaa tatcctaatg caaaaatttc ggtctacgga
     9301 cactcgttag ggtcaatgaa tgcacagtat gctattgcag aattggatga taatcaaatt
     9361 taccgagttc aaggagcttg gatatataac ggtccaaacc tttatagtat tttgacagat
     9421 aaacaaaaag aacaggttga taaaataaaa ggggtagtca ataattacgt tgattccaag
     9481 gataaaattg gaataggtta tcctaaagaa ggaagcgata atgctgtcgg aattgttaaa
     9541 atagttgaca gtaaagctgt ttcaggaggt agctgggttg ctaattttac caatcaacac
     9601 atttggggag gctaccagta caatcaagat ggttctttaa aattagcaga agataaattt
     9661 gcttacaaag aaaactattc agtagttcat acgaatgtga atcaaagaat gcatgctatt
     9721 aaagcgaaac ttagtagctt taaaagtacg ggaaaagaag ttagtaagaa tcaagctatt
     9781 ttcctagata gtcaacaagc aaaagtgatt tctagtggtt tggtaacgat atcaaaagaa
     9841 gcagttgaag aagtaaaaaa agttgagcaa aaagcagttg ctaaagcaga agagctttat
     9901 caatctttaa aagtagctcc tacaggagtt actgaactaa gtcctcaaga attggaagaa
     9961 gcgtatgctc agtcaggtgt tagctatgcg agcattgtcg gagaagctca aacctttttc
    10021 tcaaataaaa ttcaagcggc agaaagcatt tctcacgctt acgttgaatt agaaaaccaa
    10081 attaataaag gggttgaagc tatgttgcaa caagatagta acttgtcagg ggagtttaaa
    10141 caatggcact gattgatatc attgaaaaac aattagctga tactcagaga aaaatatcag
    10201 atttagacga tgcctatcac catagttgtt gccagtttga agaaaaatta gacgatctct
    10261 cagtaagaaa aaataagatt actaatatgc ttcaagaaac ttacgacgct gttgaatatg
    10321 atttgagata tagtaatgat agctcagata tgatgacgct caatcgtatt ttagattctt
    10381 atcatgacga cttagagcaa gcttaccata aagagtacta tgccttgtcc gctcaagaag
    10441 aagaatatag agctaactat attcggcaac gttcagaaca tgaactaacc tttgaagagt
    10501 tgcaaaggga aaagaaaaga gaattaatga agtaggagaa attattgagt ttaatgtggg
    10561 atgtcctgac tggtaaggag tcagtaagga ctttaaaaaa acaattggat aaagaattga
    10621 tacatagcgt gtacgcgcca cgcaagagat ctaagtttca cgatattatt acgtcgcatc
    10681 ataaagaaat agacgaagta gccatcaaca caaagacagc tattcataaa gttgctaatc
    10741 aattggacga ggcaataaaa ggagaatttc gttcaagggt tgaggaggct attgaaagca
    10801 atcagcaaaa atataatcat ttatagtcta gtttctctac cattagctag acacttgtag
    10861 aggattttta cagcaagtat tcaaaagtca gagcaaaaat gttttgactt ttttgattta
    10921 ctctttctga tcagaatgct tcagtcttat gttataataa taaagattaa tgcttgtagc
    10981 tacgataagc aaagaaagaa cagtgatatg ataaaaatat ttgataccca tacccacctt
    11041 aacgtggaaa attttgaagg aaagatagac gaggaaatta atttagctag cgaactcggt
    11101 gttataaaaa tgaatgttgt cggctttgac caagatacca tttcaaaatc tttggaatta
    11161 tcttcccaat atgcacaggt atatagcaca ataggatggc atccaactga agctggttct
    11221 tatgatgata atattgagtc aatgattatc agtcatttag aaaatcccaa ggttattgca
    11281 cttggtgaaa ttggtctgga ttattattgg atggaggatc ccaaagatat tcagatcgag
    11341 gtgtttaagc gtcaaattga actttcaaaa gagtataacc ttccttttgt cgttcataca
    11401 cgtgatgcgt tagaagacac ttatgaagtc attaaggaat ctggagttgg gccttttggt
    11461 ggtataatgc actctttttc tggttctttg gaaatggctc agaaatttat tgatttaggg
    11521 atgatgattt ccttttctgg tgttgtgacc tttaagaagg cattagatgt acaagaagct
    11581 gcacgagagt tgccattaga taagatatta gtggaaacag atgctcctta tttggctcct
    11641 gtacctaaaa gaggtcgaga aaacaaaacc gcttatacga gatatgtcgt tgaaaaaata
    11701 gcagagctac gaggcataac tgtggaagag gtagcggagg ctacatacca aaatgcagta
    11761 aggatattta gacttgatga aaaaaattga tattcaagaa gtgatcgttg tcgaagggaa
    11821 agatgatact gctaatctgc gacgctttta taatgttgat acctatgaaa ctagaggttc
    11881 agcaattgat gaagaggatt tagagcgtat cgaacgtttg cacaatttgc ggggagttat
    11941 cgtctttaca gacccagatt ataatggcga gcgtatccgt aaaatcatca tgaatgccat
    12001 tccgacagtc cgccatgcct tcctcaatcg agacgaggca aagccaggct caaagaccaa
    12061 gggacgatct ctgggtgtcg agcatgccag ctttgaggat ttgcagaagg cactctcaaa
    12121 agtgacccag catttcgatg atgaggataa ctttgatatc acccaagcag acctcattcg
    12181 ctgggggttc attaccgcta gtgatagccg taaacgccgt gagtatcttg gaaatcaact
    12241 ccgtatcggc tactcaaatg gtaaacaact cctcaaacgc ctcagactct tcggagtgac
    12301 caaggcggaa gttgaagagt gtatggaggg gtatgaggga taaaacatgt atcttatttg
    12361 tcaacgtatt aatatgattg aacaaaatga gaatccttat tttgtgaagg agtacgaaac
    12421 gggttatctt gttttgggag actatcaata ttttcaaggt tattgccttt ttctttctaa
    12481 aaaacacgta acagagttac atgaattgcc tagagattgg cgaaatcagt atctttccga
    12541 aatggcagat gcttctgaaa tagttgctaa ggcttttaga gcagataaga tgaatattga
    12601 gagtttggga aatggagacg ctcatctaca ttttcatctt tttccaagaa aaacaggtga
    12661 tttaaggaac tatgggcata atggaaaagg tccagtttgg tggtatcctt ttgagaagat
    12721 gtatgcagat agtgtcagag caactggtgc agaaatagaa aaattaaaag agaaattgtt
    12781 agatgtctta ggatagactt gagacgataa ggaaataaaa aacatgagaa tcgcagataa
    12841 aaccgttacc cgcgctattt tggagcgtca tgggtttaca tttaagaaat cattcggcca
    12901 gaactttttg accgatacga atattttaca gaagattgtt gacacggcag agattgacaa
    12961 gggtgtcaac gtgattgaga ttggtcctgg tatcggggct ttgacagagt ttttggctga
    13021 gaatgcagct gaggtgatgg cttttgagat agatgatcgt ctcattccta tccttgctgg
    13081 tacgcttgcg cgttttgaca atgtccaagt ggtcaatcag gatatcctca aggctgattt
    13141 gcagacgcaa attcaagcct tcaagaatcc tgatctgcct atcaaggtgg tcgcaaacct
    13201 gccttactac atcacaacac caatccttat gcacttgatt gagagcaaga ttccttttgc
    13261 ggagtttgtg gtcatgatgc aaaaagaagt ggcagatcgt atttcagcta tgccaaatac
    13321 taaagcatac ggttctttgt caatcgcagt gcagtattat atgactgcca aagtatcatt
    13381 tatcgttccg agaactgttt ttgttcctgc tccaaatgta gattcagtta ttttgaaaat
    13441 ggtccgtaga gatcaaccag tagtgagtgt tcaagatgag gatttctttt tcagagtgtc
    13501 aaaagtagcc ttcgtacatc gtcgtaaaac actttggaat aatctaacga gtcattttgg
    13561 aaaatcagaa gatactaaag caaaattgga aaaagcatta gaaatagcaa aaattaagcc
    13621 atctattcgc ggagaagcct tatctatccc tgattttgca agtttagcag atgccttgaa
    13681 agaggttgag atataaacta tgttggttga caagaagtgg cgttttgaag atagtgcaag
    13741 ttatttcgct tgtcctaaat gtcaaaatcc cttgataaaa gaaagtaaca gcctcaaatg
    13801 ctctggtaat cattgttttg atttatccaa atttggatat gttaatcttc ttggaggaaa
    13861 gaaggtagat gaacattatg ataaaaaatc atttgaaaat cggcagcttg tcttagaaaa
    13921 cgggtattat aatcatattt tagaagctat ttcaaaggtt ttggaaaata attctcagtt
    13981 tcattcggtt ttagatattg gttgtggaga gggcttttat tctcgtcaat tagttaacaa
    14041 gcatgagaag actttcttgg cttttgatat ttctaaagat tcgattcaat tagctgctaa
    14101 aagtgatcag tctaggcttg tgaaatggtt tgtttctgat ttagctaacc ttcctattca
    14161 agactcatca attgatatca ttttagacat tttttctcct gcaaattata aagaatttag
    14221 gcgggtgctg agtgatgatg gtatccttgt aaaggttgtt cctgtagctg aacatgttca
    14281 agaattgcga gaaaaagcta gtcaatactt gaaacagaaa gattactcta atcaaaaaat
    14341 tctagatcac tttagagaaa attttgaaat tatttctgag cagaaagttg ttcaaagcta
    14401 taactgttct caacaagaac gtcaagcttt tattgatatg acaccgttgt tattcagcgt
    14461 agataaaacg actattgatt gggcgagtat atcagaaatt acagtaggtg ccttaatagt
    14521 aattggaaaa aagcgcagtg tatctaaata agatcactgc ttttatgttg ggaattcata
    14581 aaattgaggt tatttgagag aaatcgaaca cctgcaaata gtttctattt agcttatctc
    14641 tgagagtaga gcttatttct cattttatga tataatagtg gtattctaaa caattaagga
    14701 gaacaattgc aaggtagaat tgtcaagtcg ctagcaggtt tttattatgt ggaatctgac
    14761 ggtgttgttt atcagacacg tgcgcgtgga aattttagaa aaaaaggtca aattccctac
    14821 gttggggatt gggttgaatt ttcttcgcaa gatcaatcag aaggatatat tctatcaatt
    14881 gaagagcgta aaaatagcct cgtacgacca cctattgtta atattgatca agctgtcgtt
    14941 atcatgtctg ctaaagaacc tgattttaac gctaacttat tagaccggtt cttagtactt
    15001 ctagaatata agatgattca gcctatcatt tatatttcca agttggattt gttagacgat
    15061 ttggttgtta ttgatgatat tcgcgaacat tatcagaata taggctatgt tttctgttat
    15121 tctcaagagg aactcttgcc tctgttagca gataaagtga ctgtttttat ggggcagaca
    15181 ggtgtcggaa aatcaacgct tctcaacaaa attgctcctg agttgaaact tgaaactggt
    15241 gaaatttcgg atagtttagg ccgtggccga cacaccacaa gagcagttag tttctataat
    15301 gttcataaag gaaaaattgc agacactcct ggtttttctt ctttggacta tgaagtggac
    15361 aatgcagagg acttaaacga atcctttcca gagcttcgta gacttagtca tttttgtaaa
    15421 ttccgctcgt gtacgcatac acatgagcct aaatgtgctg taaaagaagc cttgactcaa
    15481 ggtcagttat ggcaagtccg ctatgataac tacctccaat ttttaagtga aattgagaac
    15541 cgccgtgaaa catataagaa agttattaaa agaaagtagg tttctatgtc aaccaataaa
    15601 attgctccat cgatattagc agcagactat gctaattttg ctaacgaatt gaaacgtatt
    15661 gaagagacaa cagcagaata cgttcatatt gatatcatgg atggtcaatt tgtccctaat
    15721 attagttttg gtgcggatgt tgtatccagc atgcgtaaac acagtaaact tgtttttgat
    15781 tgtcatctga tggttgttga tccagaacgt tatattgagg cttttgctca agctggtact
    15841 gatatcatga ccattcatgt tgaagcaacg aaacatatac atggtgcgct tcaaaagata
    15901 aaagaggcag gaatgaaagc aggtgtggtt attaacccag gaacacctgt agaatctctt
    15961 attcctattt tagatttggt tgaccagatt ttaattatga ctgttaatcc tggctttggt
    16021 ggacaagcct ttattccaga aatgatgtca aaagtaaaga cagttgcagc atggcgcaaa
    16081 gagtacggtc atcattatga tattgaagtt gatggcggta tagataatac tacaattaag
    16141 gcagcagcag aggctggtgc aaatgtcttt gttgcaggtt cttatctctt taaagcaagt
    16201 gatttacctg cacaagtaga aaccttaagg gtggcgctcg atgactaaaa ttgctttatt
    16261 tgctggcggt gacttaactt attttgaata tgattttgac tattttgtcg gaattgaccg
    16321 tggaagccta tttttattaa aaaatgggct ttctttggac atggcagtgg gtgattttga
    16381 ttctataaca gaagatgaac tgctttatat caaacactat tgtagcaata tcgtttctgc
    16441 tagtgtagaa aaaaatgata cagatacaga attagcttta aaaactattt tcaaagaatt
    16501 tccagaagct caagtgaccg tatttggagc atttggtggc aggattgacc atatgatgtc
    16561 taatatcttt ttaccaagtg acacagattt agaaccattt atgtcacaaa tcagactcaa
    16621 agatgaacaa aacatagtga cttatctacc atccgggaaa aatcaagtct caaggattga
    16681 agggatgtcc tatgtctcgt tcatgcctga gagtgagtct actttgcaga tttctggggc
    16741 taaatatgaa ttgaacaaaa gtaattattt taagaaaaaa atgtattcca gtaatgaatt
    16801 tatgacttcg ccaattgaag tagagctcaa agatggttac ttaatcatta tttatagtaa
    16861 ggatagaggt taatatgctt gtcatcattt tgatcattgc actagctagt ctgacagtga
    16921 cgataatttc ttaccaaaaa atgacggaat taacaaagtc cgttgaaaaa caacttgaag
    16981 ataatgctga taatctatca gaccaactga cataccagat agaagcggcg caaaaagatc
    17041 aaattctaac gttgactaat cagctaaacc gtatgcaaca ggaaatttat cagttgctta
    17101 ccgatatgcg tacagaatta aatcagcatt taacggagag tcgagatcga tcagacaaac
    17161 gcttggaatt gattaactcc aatttatctc agtcagttca gaaaatgcaa gattccaatg
    17221 aaaaacgctt ggatcaaatg cgccaaactg ttgaggaaaa gctagaaaaa acgctacaaa
    17281 cgcgtttgca aacttctttt gaaactgtat cgcgtcaact agagagtgtc aatcaaggtc
    17341 ttggggagat gaaaactgtt gcccaagatg ttggtacact gaacaaagtc ttgtcaaata
    17401 ctaagacaag gggaatatta ggggagttac aactcggcca aattatagaa gatattatga
    17461 cagttagtca atatgagaga gaatttccta ctgtgtctgg ctcttctgag cgtgttgaat
    17521 atgctattaa attacctgga aatggtcagg gagattatat ctatttgcct attgactcta
    17581 agtttcctct agaagattat taccgattgg aagatgctta tgaattgggt gacaaggttc
    17641 aaatagaact ctatcgtaaa tctttactgg caagtattcg taaatttgca aaagatataa
    17701 acaataagta cttaaatcct cctgaaacga caaattttgg tatcatgttc ttaccaactg
    17761 aagggctcta ttctgaagtg gtaagaaatg caacattctt tgatagtcta agacgtgacg
    17821 aaaatattgt agtagctgga ccgtcaacct tatctgcttt actaaattca ttatctgttg
    17881 gctttaaaac cttaaatatt caaaagaatg ctaatgatat tagtaaaatt ttagggaatg
    17941 tcaaggttga atttggaaaa tttggtggca tgttatcaaa agctcaaaaa caattaaata
    18001 cagcaagtaa aagtattgat agtttactca caacgcggac aaacgctatt atcagagcct
    18061 taaatacagt tgaagaacat caagatcaag caaccactag cttgttaaac cttcctataa
    18121 cagaagaaga ggaaattaat gaaaattaac caaatgaaaa aagacgagct ttttgagggg
    18181 ttttacctta taaagaaagc agaagttcgt aaaacgcgtg caggtaaaga ctttattgcc
    18241 tttacctttc aagatgatac tggcgaaatt tcaggaaata tgtgggatgc acaaacctat
    18301 aatgtagaag agtttgtagc cggtaaaatt gttcatatga aagggcgtcg tgaagtttat
    18361 aatggaactc cccaagtcaa ccaaattaca ttaagaaaca tcaaagatgg tgaacctaat
    18421 gatcctagag attttaaaga aaaacctcca atcaatgttg acaacgtgag agagtacatg
    18481 gaacaaatgc tctttaaaat tgaaaatgca acctggcagc gtgtggtaag agcactttat
    18541 cgtaaataca ataaggaatt ttttacctat ccagccgcca aaacaaacca ccacgccttt
    18601 gaatcaggat tggcatatca cacggcaacg atggttcgtt tggcagatag tatcggagat
    18661 atctatctag aacttaataa aagtttgatg tttgctggta ttatgctaca tgatttagcc
    18721 aaggtcattg agttatcggg tcctgataat acagaatata ctattcgagg taatcttatc
    18781 ggtcatattt cacttattga tgaggaatta acaaaaatac tagcagagct taatattgat
    18841 gatacaaaag aagaggttac agtgttacgc catgttattc tgagtcatca tggacaatta
    18901 gaatatggaa gcccggttcg tcctagaatc atggaggcag aaattatcca tatgatcgat
    18961 aacattgatg caaatatgat gatgatgaca acagctttaa accgtgtcaa cgaaggtgaa
    19021 atgacaaatc gcatttttgc catggataac cgttcttttt ataaaccgaa cattaaataa
    19081 tttaggtaga ataattaaga cctaaaataa aatgttcgtt tttagcattt gttatgttat
    19141 actatatgta gagaatgcta tgaataagga gaaaatatga aattaagacg tagcgaacgc
    19201 atggttgtta tttcaaatta cctaataaat aatccctata cattaactag tttaaataca
    19261 tttgctagta aatacggtgc cgcgaaatct tcgatttcag aagatattgc tattatcaaa
    19321 aaagcttttg aacaagctca aatcggagat attaaaactg tgactggtgc aagtggaggt
    19381 gttatcttta caccaactat tgctgaagca gaagcgaaag aaatcgttga agaattaaga
    19441 cagcgtttat ctgaaaacga tcgtattctt ccgggaggtt acatttatct ttcagatcta
    19501 ctcagtacac caaaaatgtt acaaagtatt ggtcgcatta tagcgaacgc ttacagaggt
    19561 caaaaaattg atgctgtcat gacggttgca acaaaaggtg ttcctttagc aaacgcagta
    19621 gcaaatgttt tagatgttcc ttttgttatt gtgagacgtg atttgaagat tacggaaggt
    19681 tctactgtgt cagtaaacta cgctagtggt tcaagtggcc gtattgaaaa aatgtttctt
    19741 tctaagcgta gcttaaaacc taacagtcga gttttaatag ttgatgattt cttgaaaggt
    19801 ggaggtactg tttcggggat gattagttta ctctcagaat tcgatagcac attagtaggt
    19861 gtcgctgtat ttgcagaaaa tgctcaggaa caacgtgaga agatggccta taaatctctt
    19921 ctgagagtat ctgaaattga tgtaaaaaat aatagagtct ctgtagaagc ggggaatata
    19981 tttgattaaa gagtcaaggt gacatccttg attctttttg cttgtaaaag taataaaagc
    20041 ttgacacatc atgtgtaaaa tgatactatg ataaacggta cttttacttt tggtctctca
    20101 aaagtgtaca gagacgtgct gacaattgtt gcaaaagtac acgcaggtat aggctgtcac
    20161 caagtgctat atcaaccaaa aataaaaaac ttacaggaga aatgtagatg cctacaatta
    20221 accagttggt acgtaaacca cgtaaatcta aagttgaaaa atcagattca cctgctttga
    20281 atattggtta caacagtcac agaaaagttc acactaaatt atctgcacca caaaaacgtg
    20341 gtgttgcaac tcgtgttgga actatgacac ctaaaaaacc taactctgcc cttcgtaaat
    20401 ttgctcgtgt acgtttgagc aaccttattg aagttactgc ttacattcca ggtatcggac
    20461 acaacttgca agaacacagt gttgtgctta tccgtggtgg acgtgtaaaa gaccttccag
    20521 gggtacgtta ccatatcgtt cgtggtgcac ttgatactgc aggtgtagct gatcgtaaac
    20581 aaggccgttc taaatacggt gctaaacgtc ctaaagggta attagaaggg ggataagaga
    20641 aaatgagtcg taaaaatcaa gcgcctaaac gcgaagtatt accagatcca ttgtataact
    20701 caaaaatcgt tacacgtctt atcaaccgtg taatgcttga tggtaaacgt ggtactgctg
    20761 caactatcgt atatgatgct tttgaagcaa ttaaagaagc tactggaaca gatgctcttg
    20821 aagtattcga aacagctatg gaaaacatca tgcctgtact tgaggtacgc gcacgccgtg
    20881 ttggtggatc aaactaccaa gttcctgtcg aagttcgtcc agaacgtcgt acaactttag
    20941 gacttcgttg gttggttaac gcatcacgtg ctcgtggtga acacactatg aaagatcgtc
    21001 ttgcaaaaga aatcatggat gctgcaaaca acacaggagc atcagttaag aaacgtgaag
    21061 atactcacaa aatggctgaa gctaaccgtg cctttgcaca cttccgttgg taagattact
    21121 ctctttttga gacttatcat agctactaaa caaattgttt ggtagatagc aatgttagca
    21181 attagaacgg gtaggtcctg cctatccgtt ttttataaat agtgttataa taaagaagta
    21241 aataatttaa aatataggag aaaccaaatg gctcgcgaat tttcacttga aaaaactcgt
    21301 aatatcggta tcatggctca cgttgatgct ggtaaaacaa caacaactga gcgtattctt
    21361 tactatactg gtaaaatcca caaaatcggt gaaacacacg aaggtgcttc acaaatggac
    21421 tggatggaac aagaacaaga acgtggtatc acaattacat cagctgcaac aacagcacaa
    21481 tgggatggtc accgtgttaa catcatcgat acaccaggac acgtggactt cacaattgaa
    21541 gttcaacgtt cacttcgtgt acttgatggt gcggtaacag ttcttgatgc tcaatcaggt
    21601 gtagaacctc aaactgaaac agtttggcgt caagcaacag aatacggagt tccacgtatc
    21661 gtttttgcta ataaaatgga taaaatcggt gctgacttcc tctattcagt acaatcactt
    21721 catgaccgtc ttcaagcgaa tgcacaccca attcaattgc caattggttc agaagacgat
    21781 ttccgtggta tcattgactt aatcaaaatg aaagctgaaa tctatactaa tgatcttggt
    21841 acagatatcc ttgaagaaga cattccagca gagtatgttg atcaagcaaa tgaataccgt
    21901 gaaaaattag tagaagcagt tgctgatact gatgaagatt tgatgatgaa ataccttgaa
    21961 ggtgaagaaa tcactaacga agagttgatg gctgctattc gtaaagcaac tatcaacgtt
    22021 gaattctatc ctgtattgtg tggttcagcc ttcaagaata aaggtgttca attgatgctt
    22081 gatgctgtta ttgattacct tccaagccca cttgatattc ctgcaatcaa aggtattaac
    22141 cctgatactg acgaagaaga aacacgccct gcttcagatg aagagccatt tgcagcgctt
    22201 gccttcaaga tcatgacaga tcctttcgtg ggtcgcttaa cattcttccg tgtgtactca
    22261 ggtgttctta attcaggttc atacgtgttg aatacatcta aaggtaaacg tgaacgtatc
    22321 ggacgtatct tacaaatgca cgcaaatagt cgtcaagaaa ttgaaactgt ttatgctggt
    22381 gatatcgcag cggctgttgg tttgaaagat acaactacag gtgattcatt gactgatgaa
    22441 aaatcaaaag ttattcttga atcaattgaa gttccagaac cagttatcca attgatggtt
    22501 gagccaaaat ctaaagctga ccaagataaa atgggtatcg cccttcaaaa acttgctgaa
    22561 gaagacccaa cgttccgcgt tgaaactaac gttgaaactg gtgaaacagt tatctcagga
    22621 atgggtgaac ttcaccttga tgtccttgtt gaccgtatga aacgtgaatt caaagttgaa
    22681 gcaaacgttg gggcacccca agtatcatac cgcgaaacat tccgcgcttc tacacaagca
    22741 cgtggattct tcaaacgcca atctggtggt aaaggtcaat ttggtgatgt ttggattgaa
    22801 tttacaccaa acgaagaagg taaaggattc gaattcgaaa atgctatcgt cggtggtgtg
    22861 gttcctcgtg aatttattcc agccgttgaa aaaggacttg tagaatctat ggcaaacggt
    22921 gttcttgctg gatatccaat ggttgacgta aaagctaaac tttacgatgg ttcataccat
    22981 gatgttgact catctgagac agccttcaag attgctgcat cacttgcact taaagaagct
    23041 gctaaatcag cgcaaccagc tatccttgag ccaatgatgc ttgttacaat tacagctcca
    23101 gaagacaatc ttggtgacgt tatgggtcac gtgactgcac gtcgcggtcg tgttgatggt
    23161 atggaagcac gtggtaacac acaagttgtt cgtgcctttg ttccacttgc tgaaatgttt
    23221 ggttatgcaa cagttcttcg ttctgcaaca caaggacgtg gtacattcat gatggtattt
    23281 gaccactacg aagatgttcc taagtcagtt caagaagaaa tcatcaagaa aaactctggt
    23341 gaataatttc tgaaaactta tgaaaaggta ctcgaaagag tatctttttt tctttttgaa
    23401 agtggtaaat tcattgcaat ttttctcgaa aaaagatata ataattgtgt tgaaagattt
    23461 ttgcacatat gtacaagtta atcttttcac attggttagg gggatcccta aaataaaata
    23521 tttgagattt tcataaggag gaaatcacta atggtagtta aagttggtat taacggtttc
    23581 ggtcgtatcg gtcgtcttgc attccgtcgc atccaaaacg tagaaggtgt tgaagttact
    23641 cgtatcaacg accttacaga tccaaacatg cttgcacact tgttgaaata tgacacaact
    23701 caaggtcgtt tcgacggtac tgttgaagtt aaagaaggtg gattcgaagt taacggtcaa
    23761 tttgttaaag tttctgctga acgcgaacca gcaaacattg actgggctac tgatggcgta
    23821 gaaatcgttc ttgaagcaac tggtttcttt gcatcaaaag aaaaagctga acaacacatc
    23881 catgaaaatg gtgctaaaaa agttgttatc acagctcctg gtggaaacga cgttaaaaca
    23941 gttgttttca acactaacca cgatatcctt gatggaactg aaacagttat ctcaggtgct
    24001 tcatgtacta caaactgtct tgctccaatg gctaaagctt tacaagacaa ctttggtgtt
    24061 aaacaaggtt tgatgactac tatccacgca tacactggtg accaaatgat ccttgacgga
    24121 ccacaccgtg gtggtgacct tcgtcgtgct cgtgcaggtg ctgcaaacat cgttcctaac
    24181 tcaactggtg ctgcaaaagc tatcggtctt gttatcccag aattgaacgg taaacttgat
    24241 ggtgctgcac aacgtgttcc tgttccaact ggatcagtaa ctgaattggt tgcaactctt
    24301 gaaaaagacg taactgttga agaagtaaat gcagctatga aagcagcagc taacgattca
    24361 tacggttata ctgaagatcc aatcgtatca tctgatatcg ttggtatttc atacggttca
    24421 ttgtttgatg ctactcaaac taaagttcaa actgttgacg gtaaccaatt ggttaaagtt
    24481 gtttcatggt acgataacga aatgtcatac acttcacaac ttgttcgtac acttgaatac
    24541 tttgcaaaaa ttgcaaaata atttttaatt aatatgatgt aaaagaggga tttatccctc
    24601 ttttattttt ataaataaaa aataatacga atgttttttc attttgttgt agaaaattat
    24661 cagaaaagaa atgaaaactg aattatattg gctataagtt aaaataaggt ttgattttga
    24721 aagtaagcag tggtatgatt taattgaaaa aatatagagg agaagtttta tgaagactac
    24781 aaaagttttt tcagttttat cgttatctgt tgctgtattt atgatgacag catgtcagtc
    24841 aaaccacagc actaaaaaag ctgatatgtc tccagataag gtggagcaaa agacgaaatt
    24901 taaaaatatt tctctaagtt ctaataaact gttggctaag gaaaacacta tgtctgtcct
    24961 ttggtatcaa aattctgcag aagccaaggc tttatattta caaggttata atgttgctaa
    25021 aatgaagtta gatgattggt tacaaaagcc cagtgaaaaa ccatattcaa ttatcttaga
    25081 tttagatgaa acagttttag ataatagccc atatcaagca aagaatatta aagatggctc
    25141 tagtttcacg ccagagagtt gggataaatg ggtgcaaaag aaatcagcta aggctgttgc
    25201 gggtgccaaa gaatttttga agtatgctaa tgaaaaggga ataaaaattt attatgtctc
    25261 agatcgtaca gatgcccaag ttgatgcgac taaagaaaat ttagagaaag aaggtatacc
    25321 tgttcaaggg aaagaccact tgcttttcct taaaaaagga atgaaatcta aagagagtcg
    25381 ccgtcaggca gttcaaaaag ataccaattt aattatgctt tttggagata atttagttga
    25441 ttttgctgat ttttctaaat catctagtac agatagagaa caactactaa ctaaacttca
    25501 aagtgagttt ggtagtaaat ttattgtttt cccaaatcct atgtacggtt cttgggaaag
    25561 tgctatttat caaggaaaac atctggatgt tcaaaaacaa ttgaaagaac gacaaaaaat
    25621 gttgcattcg tatgattaat aagtatacga attctcttga aaacaactag gaaaatctat
    25681 gaaatctatt tgtgaaaaag taatcaataa ttacaactgt tcacaaaaat atgttataat
    25741 aaataatatc aaattatata aggagtttta ctaatggcta aattgactgt taaagacgtt
    25801 gatttgaaag gtaaaaaagt cctcgttcgt gttgacttta atgtgccttt gaaagacggc
    25861 gttatcacta acgacaaccg tatcactgcg gctcttccaa caatcaagta tatcatcgaa
    25921 caaggtggtc gtgctatcct cttctctcac cttggacgtg ttaaagaaga agctgacaaa
    25981 gaaggaaaat cacttgcacc ggtagctgct gatttagctg ctaaacttgg tcaagatgtt
    26041 gtattcccag gtgttactcg tggtgcaaaa ttagaagaag caatcaatgc tttggaagat
    26101 ggacaagttc ttttggttga aaacactcgt tttgaagatg ttgacggtaa gaaagaatct
    26161 aagaatgacg aagaacttgg taaatactgg gcttcacttg gagatggaat cttcgttaac
    26221 gatgcatttg gtacagcaca ccgtgctcac gcatcaaacg taggtatctc agcaaacgtt
    26281 gaaaaagctg tagctggttt ccttcttgaa aacgaaattg cttacatcca agaagcagtt
    26341 gaaactccag aacgtccatt cgtagctatc cttggtggtt caaaagtttc tgataagatt
    26401 ggtgttatcg aaaaccttct tgaaaaagct gataaagttc ttatcggtgg tggtatgact
    26461 tacacattct acaaagctca aggtatcgaa atcggtaact cacttgtaga agaagacaaa
    26521 ttggatgttg ctaaagacct ccttgaaaaa tcaaacggta aattgatctt gccagttgac
    26581 tcaaaagaag caaacgcatt tgctggttac actgaagttc gcgacactga aggtgaagca
    26641 gtttccgaag ggttccttgg tcttgacatc ggtcctaaat caatcgctaa atttgatgaa
    26701 gcacttactg gtgctaaaac agttgtatgg aatggaccta tgggtgtctt tgaaaaccct
    26761 gacttccaag ctggtacaat cggtgtaatg gacgctatcg ttaaacaacc aggcgttaaa
    26821 tcaatcatcg gtggtggtga ttcagcagca gctgctatca accttggccg tgctgacaaa
    26881 ttctcatgga tctctactgg tggtggagca agcatggaat tgctcgaagg taaagtatta
    26941 ccaggtttgg cagcattgac tgaaaaataa tagtcattat aatttatatt aaaggacatg
    27001 ctgatgcatg tcttttttta gttgataatg tagagttatt gaattttcct tgacttcact
    27061 tatcatttac tagtttttag atatagtggt atttggttga cactgcaatt ttcaaaagtt
    27121 tatgacgatt tcatataaat tggtttataa aagatatact agtaataaat aaaacgttta
    27181 caaaaagggt tttatggatt gatatgttag aatatatagt gaagggatag gtatgaaaac
    27241 gttaaaaaaa ttattatcta attacaaatt tgatattaaa aagtttaaat tgggaatgag
    27301 aacatttaaa acaggcttat cggtcttttt agttttattg gtttttcatt tatttggttg
    27361 gaaagggctt caaattggtg ctctaactgc tgtatttagt ttaagagaag actttgataa
    27421 aagcgtgcac tttggttttt ctagaattat tggtaatagt ataggtggct tactctcctt
    27481 agtatttttt gcattcaatg agatatttca tcaagctttt tgggttactt tattaattgt
    27541 tcctatttgt accatgttat gtatcatgat taatgttgct tgtaataata aatcaggtat
    27601 tattgggggg acagccgcat tacttattat aactttatca atcccaagcg gagagacaat
    27661 tctatacgtt tttgcacgta tttttgaaac tttttgtggt gtttttattg caatgatggt
    27721 taatacagat attgaaatac ttcggaaaaa actaaaaaat aacaaattat agtttgatgt
    27781 cataaaatct cacatgagag ttgacatcaa actttttaaa gtttataatg gtttaatgaa
    27841 aggaggccat catgaaagaa agagaactta ggcgtacaat ggctgtattc ccaattggag
    27901 ctgttatgaa gttgacagat ttgactgcca gacaaattcg ctattatgag gatcaagggt
    27961 taatcacacc tgaacgtact gaaggtaacc gccgcatgtt ttcactgaat gatatggacc
    28021 gcttactgga aataaaagac ttcattagcg atggtttgca tatttctgat attaaaaatg
    28081 aatacatgca aagacaacat aagtccaaag agaaacaaaa gtcattatca gatgccgagg
    28141 ttcgtcgact tttacaagat gagctccgta atcaagggcg tttttcaagt ccttcacagc
    28201 acattggaaa tatgcatttg taaacatgca gtcaagctag atattaagga gactatatga
    28261 ctatcacagc agaagatatt cgtcgcgaag ttaaggaaaa aaatgttacc ttcctccgtt
    28321 tgatgttcac agatatttta ggagtaatga aaaatgtcga aattccggct acagatgaac
    28381 aattagacaa agttttatca aataaagcaa tgtttgatgg atcttctatt gagggctttg
    28441 ttcgtatcaa tgaatctgat atgtatttat atcctgattt agatacttgg attgtctttc
    28501 cttggggaga tgaaaatgga gctgttgcag gtctaatctg tgatatctat acagcagaag
    28561 gagaaccttt tgccggagat ccacgtggaa atcttaagcg taatatgaaa cgtatgcaag
    28621 agatgggcta taagtcattt aacttaggac cagaacctga atttttccta tttaaaatgg
    28681 atgaaaatgg taacccaaca cttgatgtta atgacaaagg aggctacttt gacttagctc
    28741 caacagactt agcagataat acacgtcgtg aaattgttaa tgtcttaaca caaatgggct
    28801 ttgaagttga agctagtcat catgaagtag cagttggaca gcatgaaatt gactttaaat
    28861 atgatgatgt tttaaaagcc tgtgacaata ttcaattatt taaattggtt gttaaaacaa
    28921 ttgctcgtaa gcatggttta tatgctactt ttatggcaaa acctaagttt ggtatcaatg
    28981 gttcaggtat gcattgtaat atgtctcttt ttgataatga agggaacaat gctttctttg
    29041 atccagaaga tccacgtgga atgcaattat cagaggatgc ctactacttt ttaggtgggt
    29101 taatgaaaca cgcttacaat tacacagcta tcattaatcc aactgttaat tcttataaac
    29161 gtttggtacc aggatacgaa gcaccagttt acattgcttg ggcaggtcgc aatcgttcgc
    29221 cacttattcg tgtacctgca tctcgaggta tgggaacacg tctagaattg cgctcggttg
    29281 acccaacagc aaatccttat cttgctttat cagtactttt gggatcagga cttgagggaa
    29341 tcgagaataa aattgaagca ccagaaccaa ttgagacaaa tatctatgct atgactgtag
    29401 aagaacgtcg tcaagcaggt attgttgatt taccatctac tcttcataat gctcttaaag
    29461 cacttgaaga agatgaagtg gtaaaagcgg ccctaggaac tcatatctat actaatttct
    29521 tagatgctaa gcgtattgaa tgggcaagtt atgcgactta tgtttcacaa tgggaaattg
    29581 ataattacct agatttatat taatcataag aatgtttata actagtttta ggctttattt
    29641 tgtaaagtct aaaactagtt tttttgtaaa ttattgtaaa aaattgccta aaacttgtta
    29701 caggttttca tttgtctgtt atatggattt gataaactag gagtcatcag atggaggaaa
    29761 acatgaacat aaaacaatta aagtctaaaa caatgcttgg aacagtggct ttggtgtccg
    29821 ctttttcatt tgcttcaaca aatgcagatg cgaatacata taactatgca gtagatgtag
    29881 actacctagc aagtgcggag gaaattgctc aagcacatcc agctagtaat acgtttccac
    29941 taggtcaatg cacatggggt gtaaaggaga tggcaacatg ggcaggtaac tggtggggaa
    30001 acggtggtga ttgggctgcg agcgccgcat cagctggtta tacagtagga acacaacctc
    30061 gcgtgggttc tatcgtatgt tggactgatg gtagttatgg acatgtggct tatgttacag
    30121 ctgtagatcc tgtcactaat aaaattcagg ttttagaatc taattatgca ggtcatcaat
    30181 ggattgataa ttatcgtggt tggtttgatc cacaaaatac agtaacacca ggggtagtta
    30241 gctacattta tccgaattaa tctataaaga actcctcaca tagacggtat aaaagtacga
    30301 tactgtttgg atggggttct tttatattct atagttatcc cagaagtaat ttgatatact
    30361 aacttatttt gctaatatag gcaattaaaa agaacggaaa aggtattttc cgttcttttg
    30421 tttatttatc aggggtaagg accataggaa taatgatagg ctcacgctca gttttttcat
    30481 ataagaatgg tcgtaaggca ttaacaatag ctccatttac tgattggata cttgcatctt
    30541 tatttttcag tgcaatacga atagcattga ataagatatg ttggctttcg cgaataaggt
    30601 ccccagattc tctcatatag ataaagcctc ggctaaggat gtctggtcca gctaaaatca
    30661 tttttgaatc gaaatcaacg gtagcaacgg caaggacaac accatcttca gataagtcgt
    30721 ggcggtcacg tagaactgcc gcaccaatat cgccaatacc attcccatca acgtagatat
    30781 cttgagcatt gaaatgtcca gcaatgcgag cagagtcgct cgttaaagct aaaacatcac
    30841 cgttttccat aatgaagata ttctctttag gaatacctgt gtcaactgct aaaccagcat
    30901 ggactttttg catgcgatac tcaccgtgta cgggcatgaa atactttggt ttaatcaagc
    30961 gtaacattaa tttttgttct tgttgaccac catggccgga ggtatggata ttgttaattt
    31021 taccatgaat aacatcgaca ccagcttctt gaatggtatt gattaattta ttgacacttg
    31081 ttgtattacc agggattggg cttgatgaga aaatcacagt atcaccaggt tgcaaagtaa
    31141 cttgacgatg agtaccgtta gcgatacgag caagtgctgc catagactca ccttgactac
    31201 ctgtacacat aattaaaact tcactagcat gaagatgttt aagttcgctt ggctcaatga
    31261 aggtaccttt tggaacttta atatagccaa gttcaatacc gttaacaatt gccttttcca
    31321 ttgaccgtcc aaaaacagca attttacgac cagttttaac agcagcttca gcagcctgtt
    31381 ggagtcggaa gatgtttgaa gcaaaagagg cgaaaataat acgtccctcg ataccttcga
    31441 taatcttcat aatagattgc ccaacaattt tttcggaatt agtgaaggtt ggaacttcag
    31501 catttgttga gtctgataaa aggcagagga caccgtcttc acctagagca gccatacgat
    31561 gtaagtcagc gggctcccca acaggtgtaa agtcaaattt gaagtcacct gtacaaataa
    31621 ccttaccttg aggggtatga ataacaatac ctaatggttc aggaatggag tgagtagtgc
    31681 ggaaaaatgt aacgcttaag tttttaaaag tcagctctgt attagcgtgg atttcgtata
    31741 atgtagcgtc acgaagtaaa ccatgttctt ctaacttacc tttgattaga gcaagagcga
    31801 gagggcctgc ataaatcggt aaattagctt gttttaatag aaaaggaata ccaccgatgt
    31861 ggtcctcgtg cccatgagtt ataacaaggg ccttgatacg gtcaatgttt tcaacgatat
    31921 atgaatagtc tgggataaca taatctattc ccaaaagatc atcttcaggg aatttaatac
    31981 cagcgtctac aataataatt tcgtcttgat attcgatacc gtaagtattt tttccgattt
    32041 caccgagacc accaatcgca taaaccccaa cttcttcggg ttttaagttg atatttgaca
    32101 taataagttt atcataatgg gtagattact ctagctatta tgtttaactc ctttctagaa
    32161 ctcagtgagc tcgaaaacac cagtttcttt ttcatattca aggtgtttgt ctgaaagtaa
    32221 ctcgatgaat tcgatattgt aagctgtatt ttcttcaact agtttacgtg cagcaatgcg
    32281 accttctaat tcattagctg catcaatagt aatgtaaagt gttttggttt gctcacgacg
    32341 tgggttgcgt tcttttgttt cttgatagaa aactttataa atcataataa tcctttctta
    32401 tctctcggtt tttctaacta taaaaaaatg ggtcaatgcc gttaagtgag acaatgacaa
    32461 accattatta agtttttata gagtaataga attaaccttg attcgttgca attatcatga
    32521 ttatatcata aaataagagg tagaatcaag taagggaagt taaatgatga aagttttagc
    32581 ctttgatact tcaagcaaag cactatcagt ggctgtacta aacaatatgg aatgtttagc
    32641 gactgtcact atcaatatca aaaagaatca tagcattaat ttgatgccag ccattgattt
    32701 tttaatgcaa tcaattgatt tagaacctca agatttggac cgtatcgtag tagcagaggg
    32761 tccaggatct tatacgggct tacgtgtagc tgttgctaca gcaaaaatgc tagcttatac
    32821 gcttaagatt gacttagttg gagtatctag cctgtacgct ttaacaaatg gattttcaga
    32881 aaatgattta ttggtaccac ttatagatgc acgacgtaac aatgtttatg ttggtttcta
    32941 tcaaaatgga gatactgtta aaccagactg tcacacttct cttgaagaag tcttacaaga
    33001 ggtggggaat aaagccaatg ttcattttgt cggagaggtt gcagcatttg ttgaccagat
    33061 taagaaagtt ttaccacatg ctaaaattac agaaacttta ccttgtgcag tggcaattgg
    33121 gcgcaaagga caaaaaatga aaagcgttaa tgtagatgcg tttgttccac gatacttaaa
    33181 acgtgttgaa gctgaggaaa attggttaag aaaccactgt gaaacgaata cagaagaata
    33241 tattaagaga gtttgagatg gaatcatcag aacaagcact ggctatatgg agcgtactat
    33301 ctgatgtcta tgacaaatct ccgtggagtt tatcgcagat ttcagaagat ttaaaaaaag
    33361 attcaacaga ttattttttt gtttataatg acggtgaagt cattggtttt ttggctcttc
    33421 aacaattggt aggtgaagta gaaattacta atattgctgt taaaaaaaat tatcaaggta
    33481 aaggttatgc ttaccagtta atgtcaatga tcgctgatat agaggtgcca gtttttcttg
    33541 aggttcgtta ttcaaatatc gtagcacaaa aactttatga gaggtgtggt tttgtagttt
    33601 taggaaagcg caaaaattat taccatgatc caattgaaga tgctatagtg atgcgcaagg
    33661 gataaatagt tgatgataga aaggatatga atagtcctca atactaatgg aaaagacgat
    33721 attgatggat taatatcatt atgaaagata gatatatttt agcagtggaa agttcatgtg
    33781 atgaaactag tgttgctatt ttaaaaaatg ataaagaatt actagctaat attattgcaa
    33841 gtcaagttga aagtcacaaa cgttttggtg gtgttgttcc tgaagtggca agccgtcatc
    33901 acgttgaagt agtaacaacc tgttttgagg atgctcttca agaagcaggt attgttgcta
    33961 gcgatttgga tgctgttgct gtaacatatg gtccgggatt agtaggagcc ttattggtag
    34021 gtatggctgc agcaaaagct ttcgcttggg caaataaatt acctctaatt cctgtaaacc
    34081 acatggcggg tcatttaatg gcagcacgtg acgttaagga acttcaatac ccattgttag
    34141 ctttgcttgt cagtggagga catacagaat tagtatatgt ttctgaaccg ggagattaca
    34201 aaatagtagg agaaactcgg gatgatgctg ttggagaagc ttatgataaa gtaggccgtg
    34261 ttatgggctt aacttatcca gcaggtcgcg agattgatca gttagctcat aagggtcaag
    34321 atacttacca ttttcctaga gcgatgatca aagaagatca tcttgaattt tctttttctg
    34381 gattaaaatc tgcatttatc aatttacatc ataatgcaga acaaaagggt gaagcattgg
    34441 ttcttgaaga tttatgtgct tcctttcagg cggctgtttt ggatatttta ttggctaaaa
    34501 ctcaaaaagc tttgttaaag catccagtga aaactttagt cgttgctggt ggagttgcag
    34561 ctaatcaagg acttcgggaa cgcttggcta ctgatatttc tcctgatatt gatgtggtta
    34621 ttcctcctct tagattatgt ggggataatg caggaatgat tgcattagca gcagcgatag
    34681 agtttgaaaa agagaatttt gcttctttaa aattgaatgc caaacctagc ttagcttttg
    34741 agagtttata gtgttcataa tatcaaaacg cttgttttga tattttttat ttgttataat
    34801 gattgaatat tcaaaaatat tagcaaatga tattgtcgct atttttttaa ctaataaagg
    34861 agttaaggaa tgttacattt tgaaaatgat tataataaag gagctcatcc agagcttttg
    34921 aatgccctta ttgaaactaa tgatcaaggt ctatcgggat ataggacaga tagttactgt
    34981 caacaagcgg cagataaaat tcgagaagtc tgtttctgtc cacaagctga agttgagttt
    35041 ttagtgggtg gtactcaaac aaatcaagta gttatcagta gtatgcttgc ttcttatgaa
    35101 ggagttattg ctgcagaaac aggtcatgtg agtagtcatg aagcaggagc catagaattt
    35161 tcaggtcata aggtattgac tttaccttct cataatggga aattactcgc aagcgaagtt
    35221 gcaacttata ttgagacttt ttatgctgat ggtaattacc aacacatggt ttttccagga
    35281 atggtttata tatcacatcc aacagaatat ggaacacttt atagtaaagc agaactagaa
    35341 gagttgtcga agatttgtaa acattatcaa atccccttat ttattgacgg tgctcgtctg
    35401 gggtatggtt tggctgcgaa agatacagat gttgatttcc caacgattgc agccttatca
    35461 gatgtttttt atattggcgg aactaaaatg ggggctcttg ctggggaagc agttgttttc
    35521 acaaagaaaa atcgtccaaa acagtttact acaattgtaa agcagcatgg tgcgttatta
    35581 gccaagggaa gattgctggg acttgctttt gatcgtttct ttacggatga cctttactta
    35641 aaaattggaa agcatgcgat cgacttagca gaggaattaa aaatcatttt agaagaaaaa
    35701 ggttactcat tctatttaaa atcaccaact aatcaacaat ttattattgt agaaaataca
    35761 aaactagcag atttagcaaa gaatgtagct tatagttttt gggaaaaata tgatgaccat
    35821 catactgtaa tacgtctagc aactagttgg tcaactagtc gcgaagatgt aact
//
LOCUS       DATRNK010000014        35018 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.014, whole genome shotgun sequence.
ACCESSION   DATRNK010000014 DATRNK010000000
VERSION     DATRNK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35018
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.014"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            694..1818
                     /gene="mutY"
                     /locus_tag="VBC52_000698"
     CDS             694..1818
                     /gene="mutY"
                     /locus_tag="VBC52_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEO6882299.1"
                     /translation="MWPEDRIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YGKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNRSNPIIWQTQNETFQREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFSLYPFATPQKKMLENYLKQKNA"
     gene            1995..2588
                     /locus_tag="VBC52_000699"
     CDS             1995..2588
                     /locus_tag="VBC52_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6882300.1"
                     /translation="MEIGQQIIRYRKQQALSQEELAEKVYVSRQSISNWENDKTYPDI
                     HSLLLLSQIFQVSLDQLIKGDIEKMKYTITQVDKKNFERDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQTYKEILAVSNGKLLDEIEKREE
                     RAKLPYQKPLIVTVFFLITIATFFASRFIFTWLFHLS"
     gene            complement(2634..2948)
                     /gene="trxA"
                     /locus_tag="VBC52_000700"
     CDS             complement(2634..2948)
                     /gene="trxA"
                     /locus_tag="VBC52_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEO6882301.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(3029..3529)
                     /locus_tag="VBC52_000701"
     CDS             complement(3029..3529)
                     /locus_tag="VBC52_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6882302.1"
                     /translation="MESYEQFYAKLSQPFRKKPQLIILLNFLLKIVTGMMYILYPSFL
                     IFTLWQGMTFQLWLRILIIPAVGFIALSYIRKRLDFPRPYEKWNIKPLIYKDTEGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGVHYPKDVIVGYLIGLIL
                     GLCLFI"
     gene            complement(3533..5872)
                     /locus_tag="VBC52_000702"
     CDS             complement(3533..5872)
                     /locus_tag="VBC52_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEO6882303.1"
                     /translation="MNNKILEQLEFNKVKELILPYLKTEQSQEELSELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEESRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMTDNKATIPEISNDSTLALINVRHPLLSNPVANDLHFDQDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADHNSLVLFDELGAGTDPQEGASLAMAILEHLRLSNIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFTLVRVQEEQ
                     KVKSKQINVVRKADSSGPRARLDLRGKRYEEAMQELDNFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG"
     gene            complement(5957..6499)
                     /locus_tag="VBC52_000703"
     CDS             complement(5957..6499)
                     /locus_tag="VBC52_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEO6882304.1"
                     /translation="MLSLLLLIIVIWHFYIGYSRGIFLQVFYVLMSMVSLMIASQFYQ
                     ELASQITLWVPYSNPVQGVEVYFFKDISKFQLSHVYYAGVAFVFIYSLSYLVGRLLGV
                     LLHLAPVEHFDSLQNNIISGFLAVLVCLLFMSMCLTILATVPMSFVQEKLWNSLFVRF
                     LINDLPFFSQFLVRTWAVGL"
     gene            complement(6502..6813)
                     /locus_tag="VBC52_000704"
     CDS             complement(6502..6813)
                     /locus_tag="VBC52_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882305.1"
                     /translation="METKINNSQKNRYKFVFGDKPLTLTTDKDNLFMEEIERVATEKY
                     EAIKEKLPNADNETIAILMAINALSVQLSREIDIEKMEDELNKLRSKTISDIKEKVSE
                     D"
     gene            6924..7817
                     /locus_tag="VBC52_000705"
     CDS             6924..7817
                     /locus_tag="VBC52_000705"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEO6882306.1"
                     /translation="MNTIVMQADKKLQEKIRTDLAQHHISNNNPYVVFSAKISGTTVL
                     LYTSGKLVFQGSNASHIAQKYGYIEQKESCSSESQDIPIIGTDEVGNGSYFGGLAVVA
                     SFVTPKDHAYLKKLGVGDSKTLTDQKIKQTAPLLEKAIPHKALLLSPQKYNQVVGPNN
                     KHNAVSVKVALHNQAIFLLLQDGFEPEKIVIDAFTSSKNYQNYLKNEKNQFKQTITLE
                     EKAENKYLAVAVSSIIARNLFLENLNKLSDDVGYKLPSGAGHQSDKVASQLLKAYGIS
                     SLEHCAKLHFANTKKAQALLK"
     gene            7833..8426
                     /gene="lepB"
                     /locus_tag="VBC52_000706"
     CDS             7833..8426
                     /gene="lepB"
                     /locus_tag="VBC52_000706"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO6882307.1"
                     /translation="MKEFIKEWGIFILILSLFLLSRIFLWQFVKVDGHSMDPTLADKE
                     QLVVLKQTKINRFDIVVANEEEGGQKKKIVKRVIGMPGDVIKYKNDTLTINNKKTEEP
                     YLKEYTKLFKKDKLQEKYSYNPLFQDLAQSSTAFTTDSNGSSEFTTVVPKGHYYLVGD
                     DRIVSKDSRAVGPFKKSTIVGEVKFRFWPIRRFGTIN"
     gene            8555..10975
                     /locus_tag="VBC52_000707"
     CDS             8555..10975
                     /locus_tag="VBC52_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEO6882308.1"
                     /translation="MEVFFTGTIERIIFENASNFFKILLLEIEDTDSDFDDVEVIITG
                     TMADVIEGEEYTFWGTLTQHPKYGEQLQSVRYERAKPTSGGLVKYFSSEQFKGIGKKT
                     AQRIVELYGDNTIDKILESPEQLSTISGLSKINREAFIAKLKLNYGTEQVLAKLAEYG
                     LSNRAAIQIFDHYKEESLEVINENPYQLVEDIQGIGFKIADQLAEQVGIESDSPKRFR
                     AAIIHTLVESSMEQGDTYIEARTLLEKTITLLEEARQIELDPSIVAKELTNLIAEDKV
                     QHIGTKIFSNTLFFAEEGIKKNLQRILNQPFDKQLSHKDIDREIRDIQKSLNIHYDNI
                     QEKAIREALLSKVFILTGGPGTGKTTVINGIIEAYSELHHIDLNKNDIPIVLAAPTGR
                     AARRMNELTGLPSATIHRHLGLNGDSDYQSLDDYLDCSLIIIDEFSMVDTWLANQLFD
                     ALDSHTQVIIVGDSDQLPSVGPGQVLADLLNINALPHVKLEKIFRQSEESTIVTLANQ
                     MRQGFLPEDFTAKKADRSYFEASANIIPNMISKIVQSALKSGIEAHEIQILAPMYRGQ
                     AGINNLNLIMQNLLNPLKDNNQFTFNDINFRIGDKVLHLVNDTELNVFNGDIGYITDL
                     IPAKYTESKQDEIYMTFDGQEVIYQRKEWLKITLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRMLQRNLIYTAITRSKSKLILLGEIGAFDFAVKNEGAKRNTYLIERFENKQEIANS
                     QKIEDSSIDQKIDSTIINTSIPKTATPIEQTNLSKITYRLTEENYLTIDPMIGINQQD
                     ISAIFDSK"
     gene            11088..11570
                     /locus_tag="VBC52_000708"
     CDS             11088..11570
                     /locus_tag="VBC52_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882309.1"
                     /translation="MVSYEKVRRSLRTATITIIVLNSLSLVFRLFTGISVQLAKTEIN
                     KGNTGNLPKEHIEAVLSATTPFMLFVTALIVLVNIAIVIFCIKNLRAIKRNQMVNYLP
                     YYLGFAITVGLVILGFLTTKAPWAIAINIVFQAIFGLLYFHAYQKAQKLNERDLEETN
                     "
     gene            complement(11641..12750)
                     /gene="dinB"
                     /locus_tag="VBC52_000709"
     CDS             complement(11641..12750)
                     /gene="dinB"
                     /locus_tag="VBC52_000709"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEO6882310.1"
                     /translation="MGERTMLIFPLINDTSRKIIHIDMDAFFASVEERDNPSLKGKPV
                     IIGSDPRKTGGRGVVSTCNYEARKFGVHSAMSSKEAYERCPQAIFISGNYQKYRQVGM
                     EVRDIFKKYTDLVEPMSIDEAYLDVTENKMGIKSAVKLAKMIQYDIWNDVHLTCSAGI
                     SYNKFLAKLASDFEKPKGLTLILPDQAQDFLKPLPIEKFHGVGKRSVEKLHALGIYTG
                     EDLLSLSEISLIDMFGRFGYDLYRKARGINASPVKPDRVRKSIGSEKTYGKLLYNEAD
                     IKAEISKNVQRVVASLEKNKKVGKTIVLKVRYADFETLTKRMTLEEYTQDFQIIDQVA
                     KAIFDTLEESVFGIRLLGVTVTTLENEHEAIYLDF"
     gene            12920..15232
                     /gene="pflB"
                     /locus_tag="VBC52_000710"
     CDS             12920..15232
                     /gene="pflB"
                     /locus_tag="VBC52_000710"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEO6882311.1"
                     /translation="MATVKTNTDIFEQAWEGFKGVDWKEKASIARFVQANYAPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEETRFPMDTRVASISELPAGFIDKDNELIFGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYATTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLAVYGADYLMQEKVNDWNALNDIDEESIRLREEI
                     NLQYQALGEVVKLGDLYGVDVRKPAMNTKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFARTKAYDALYSGDPTFITTSMA
                     GMGADGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWSDQLPYAFRRYCMSMSHKHSSI
                     QYEGVSTMAKEGYGEMSCISCCVSPLDPENEDKRHNLQYFGARVNVMKALLTGLNGGY
                     DDVHKDYKVFDIDPIRDEVLNFDTVKANFEKSLDWLTDTYVDAMNIIHYMTDKYNYEA
                     VQMAFLPSHVRANMGFGICGFANTVDSLSAIKYATVKPIRDEDGYIYDYETVGDFPRY
                     GEDDDRVDSIAEWLLEAFHGRLAKHKLYKDAEATVSLLTITSNVAYSKQTGNSPVHKG
                     VYLNEDGSVNLSKVEFFSPGANPSNKAKGGWLQNLNSLSKLDFAHANDGISLTTQVSP
                     RALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLKDVYDKIMNGEDVIVRISGYCV
                     NTKYLTPEQKTELTQRVFHEVLSMDDALTN"
     gene            15332..15706
                     /locus_tag="VBC52_000711"
     CDS             15332..15706
                     /locus_tag="VBC52_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEO6882312.1"
                     /translation="MLNHKFLQVLKYEGVVSITSWTELAPHVTNTWNSYLTITDDQRI
                     LAPAAGMTHLENDLNNNSNIIMTLGSREVEGRDGYQGTGFRIEGTAKLLEAGSDFEIV
                     KEKYPFLRKVLEVTPINVIQLL"
     gene            complement(15741..16670)
                     /locus_tag="VBC52_000712"
     CDS             complement(15741..16670)
                     /locus_tag="VBC52_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEO6882313.1"
                     /translation="MIKTNTVLELIKEQIKDNLYHGASLAIYENGEWHEHYLGTIDGN
                     EKVKAGLVYDLASVSKVVGVGTLLAKLVYHGTIDIDKPLRYYYPRFHHQTLTVRQLAT
                     HSSGIDPFIPNRDQLNATQLKDAINHIKVLEDKSFKYTDINFLLLGFMLEEVLGDGLD
                     KLFKRYIFTPFQMKETSFGPRVEAVPTVAGINDGIVHDPKAKVLGKHTGSAGLFSTID
                     DLQRFSIHYLKDDFAKPLWNNYSLSKSRSLAWDIDKDWINHTGYTGPFIALNYQKQAA
                     AIFLTNRTFSYDDRPLWIEKRRHIQEAITSCFE"
     gene            complement(16667..17422)
                     /locus_tag="VBC52_000713"
     CDS             complement(16667..17422)
                     /locus_tag="VBC52_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEO6882314.1"
                     /translation="MTLFHSLTFKHPVLRVNNRDLNITFYQESLGFKLISEENAIAVF
                     SAWQNKEASFIIEESPTYRTRAVNGTKKLAKIIVKSQDAKDIEKLLANGAQAIQVYQG
                     QNGYAYETVSPEGDLFLLHAEDDLSQLVAIERPELEKNDDTTGLSNFVFQSISLNVPD
                     AVKAEAFYDKVFAGKFPINLSFKEAQGQDLQIAPNETWDIEILECCVNEDTNLNDLKS
                     TFESLGLDVYLDSKEKILVISDTSNIEIWISKE"
     gene            17547..18443
                     /locus_tag="VBC52_000714"
     CDS             17547..18443
                     /locus_tag="VBC52_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEO6882315.1"
                     /translation="MNETLLLHGIQLILIIAMIITFYQIVRHIRSQKINPFKRFFTGL
                     WIGFVTDALDTLGIGSFATTTTFFKLTKLVEDDRKIPATMTAAHVLPVLLQSLCFIFV
                     VKVEALTLITMAGAAFIGAFVGAKMTKNWHAPTVQRILGTLLITAAIIMLYRMITNPG
                     AGISDSVHGLHGIWLFVGIVFNFIIGVLMTMGLGNYAPELIFFSLMGLSPAVAMPVMM
                     LDAAMIMTASSTQFIKSGRVNWNGFAGLVTGGILGVIVAVLFLTNLDLNSLKTLVVGI
                     VLFTGAMLIRSSFKKTMPKSHH"
     gene            complement(18521..19369)
                     /locus_tag="VBC52_000715"
     CDS             complement(18521..19369)
                     /locus_tag="VBC52_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6882316.1"
                     /translation="MIEITWTVKYITEFIATAFLIILGNGAVANVDLKGTKGNNSGWI
                     IIAIGYGLGVMMPALMFGNVSGNHINPAFTLGLAFSGLFPWAHVGQYILAQILGAMFG
                     QLVVVMVYQPYFVKTENPNHVLGSFSTISALDDGQKSSRKAAYINGFLNEFVGSFVLF
                     FGALALTKNYFGVELVGKLVQAGYDQTTAATRISPYVTGSLAVAHLGIGFLVMTLVAS
                     LGGPTGPALNPARDLGPRIVHRLLPKQILGQAKEDSKWWYAWVPVLAPIVASILAVAL
                     FKLLYL"
     gene            complement(19538..19990)
                     /locus_tag="VBC52_000716"
     CDS             complement(19538..19990)
                     /locus_tag="VBC52_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO6882317.1"
                     /translation="MTQKYERILIAIDGSYESELAVEKGINVALRNDAELLLTHVIDA
                     HAYQSEGVFSDYVFDRQEQESADVLAYFEKLAHSKGLTKIKKITEIGNPKTLLAKDIP
                     IREKADLIMVGATGLNTFERLLIGSTSEYILRHSKVDMLVVRDSKKTL"
     gene            complement(20008..21210)
                     /locus_tag="VBC52_000717"
     CDS             complement(20008..21210)
                     /locus_tag="VBC52_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6882318.1"
                     /translation="MEDKLFNKHFIGITILNFIVYMVYYLFTVIIAFIATKELGVSTS
                     QAGLATGIYIVGTLIARLIFGKQLEVLGRKLVLRGGAIFYLLTTLAYFYMPSIGVMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPADKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HINFKMVIVLCSILIAIVVLGAFVFPVKNITLNPEQLAKSKSWTIDSFIEKKAIFITI
                     IAFLMGISYASVLGFQKLYTTEINLMTVGAYFFIVYALVITLTRPSMGRLMDAKGDKW
                     VLYPSYLFLTLGLALLGSAMGSVTYLLSGALIGFGYGTFMSCGQAASIKGVEEHRFNT
                     AMSTYMIGLDLGLGAGPYILGLVKDGFLGAGVQSFRELFWIAAIIPVVCGILYFLKSS
                     RQVETKTI"
     gene            21366..22025
                     /locus_tag="VBC52_000718"
     CDS             21366..22025
                     /locus_tag="VBC52_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO6882319.1"
                     /translation="MNHQILQNYINSHNLPIIEKDYHKYLTFESLEEDYTYILKDGIV
                     KQSVLSKYGTEFNLRYVTGLEITSILNTDYSQHMGEPYNVRIESETAHFYKVRRSTFL
                     KDINNDIELQGYVKDFYHNRLEKSMKKMQCMLTNGRIGAISTQLYDLSKMFGEERDNG
                     DIYINFVITNEELGKFCGISTGSSVSRILKQLKDDHIIRIEKQHIIITNVEKLKDHIV
                     F"
     gene            22049..24334
                     /locus_tag="VBC52_000719"
     CDS             22049..24334
                     /locus_tag="VBC52_000719"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEO6882320.1"
                     /translation="MRYNQFSYIPTKPNEAFEELKGLGFPLNKKNSDKANLEAFLRHS
                     FLNQTDTDYALSLLIVDAKTDALTFFKSNSDLTLENLQWIYLQLLGFVPFVDFKDPKA
                     FLQDINFPVSYDNIFQSLHHLLACRGKSGNTLIDQLVADGLLHADNHYHFFNGKSLAT
                     FNTNQLIREVVYVETSLDTMSSGEHDLVKVNIIRPTTEHTIPTMMTASPYHQGINDPA
                     ADQKTYQMEGALAVKQPKHIQVDTKPFKEEVKHPSKLPISPATESFTHIDSYSLNDYF
                     LSRGFANIYVSGVGTAGSTGFMTSGDYQQIQSFKAVIDWLNGKVTAFTSHKRDKQVKA
                     DWSNGLVATTGKSYLGTMSTGLATTGVEGLKVIIAEAAISTWYDYYRENGLVCSPGGY
                     PGEDLDVLTELTYSRNLLAGDYIKNNDCYQALLNEQSKAIDRQSGDYNQYWHDRNYLT
                     HVNNVKSRVVYTHGLQDWNVKPRHVYKIFNALPQTIKKHLFLHQGQHVYMHNWQSIDF
                     RESMNALLSQELLGIDNHFQLEEVIWQDNTTEQTWQVLDAFGGNHQEQIGLGDSKKLI
                     DNHYDKEAFDTYCKDFNVFKNDLFKGNNKTNQITINLPLKKNYLLNGQCKLHLRVKTS
                     DKKAILSAQILDYGPKKRFKDTPTIKFLNSLDNGKNFAREALRELPFTKDHYRVISKG
                     VLNLQNRTDLLTIEAIDPEQWFDIEFSLQPSIYQLSKGDNLRIILYTTDFEHTIRDNA
                     SYSITVDLSQSYLTIPTNQGN"
     gene            24338..24697
                     /locus_tag="VBC52_000720"
     CDS             24338..24697
                     /locus_tag="VBC52_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882321.1"
                     /translation="MKLLTKELFDDSQHFWYQINLSQESNFGAVFDHDNKNIPQVVAT
                     IVDDLQGSGSSNHFWYFGNTTDTSILMIAHLNRKFYIQVNLKDFDFALNLITINNWKS
                     LLQTQLEALNDTLAIFQ"
     gene            24743..25723
                     /locus_tag="VBC52_000721"
     CDS             24743..25723
                     /locus_tag="VBC52_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO6882322.1"
                     /translation="MSSYWNNYPELKKNIDETNQLIQERIQVRNKDIEAALSQLTAAG
                     GKQLRPAFFYLFSQLGNKENQDTQQLKKIAASLEILHVATLIHDDVIDDSPLRRGNMT
                     IQSKFGKDIAVYTGDLLFTVFFDLILESMADTPFMTINAKSMRKILMGELDQMHLRYN
                     QQQGIHHYLRAISGKTAELFKLASKEGAYFGGAEKEVVRLAGHIGFNIGMTFQILDDI
                     LDYTADKKTFNKPVLEDLAQGVYSLPLLLAIEENPDIFKPILDKKTDMATEDMEKIAY
                     LVVSHRGVDKARHLARKFTEKAISDINKLPQSSAKKQLLQLTNYLLKRKI"
     gene            complement(25808..27556)
                     /gene="cydC"
                     /locus_tag="VBC52_000722"
     CDS             complement(25808..27556)
                     /gene="cydC"
                     /locus_tag="VBC52_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEO6882323.1"
                     /translation="MFKIPLFKELKTDQWVKPFFKQYKVSLVIALFLGFMTFFSASAL
                     MFNSGYLISKSASLPSNILLVYVPIVLTRAFGIGRPVFRYIERLTSHNWVLRMTSQLR
                     LKLYHSLESNAIFMKRDFRLGDVMGLLAEDINYLQNLYLRTIFPTIIGGLLYSFIIIA
                     TGFFSLWFALMMLLYLAIMIFVFPLWSILANGARQTREKELKNHLYTDLTDNVLGISD
                     WIFSQRGQEYVALHERSESELMAVQKKIRSFDNRRALIVELVFGFLAILVIIWASNQF
                     IGHRGGEANWIAAFVLTVFPLSEAFAGLSAAAQETNKYSDSIHRLNELSETYFETTQN
                     QLPNKPYDFSVKNLSFQYKPQEKWVLHHLDLDIKEGEKIAILGRSGSGKSTLASLLRG
                     DLKASQGEITLGGADVSIVGDCISNYIGVIQQAPYLFNTTLLNNIRIGNQDASEEDVW
                     KVLERVGLKEMVTDLSDGLYTMVDEAGLRFSGGERHRIALARILLKDVPIVILDEPTV
                     GLDPITEQALLRVFMKELEGNTLVWITHHLKGIEHADRILFIENGQLELEGSPQELSQ
                     SSQRYRQLKAADDGDL"
     gene            complement(27549..29267)
                     /gene="cydD"
                     /locus_tag="VBC52_000723"
     CDS             complement(27549..29267)
                     /gene="cydD"
                     /locus_tag="VBC52_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEO6882324.1"
                     /translation="MLDKAVMRLSGIHKLLGLLAGLDVLQAIFIIGQAYYLSLSITGL
                     WEGQKLSSQTVYILLFMVSYLGRHVIDYIKNRKLDDFSTAQSSLLRRQLLDKLFDLGP
                     KVVQEQGTGNVVTMALDGVSLVENYLRLVLNKMINMSIIPWIILAYIFYLDIESGAIL
                     LIVFPLIIIFMIILGYAAQAKADKQYESYQVLSNHFLDSLRGIDTLKYFGLSKRYGKS
                     IYQTSESFRKATMSTLKIGILSTFALDFFTTLSIAIVAVFLGLRLLNEQIYLLPALTI
                     LILSPEYFLPVRDFSSDYHATLDGKNAFQAIQKVLNKTGIKGEQLVIDDWSKESHLDL
                     ENIAIAYDQKRVVEDVTLRFRGHQKVALVGVSGSGKSSLINLLSGFLGPDKGSLKVDG
                     REVVNLDQEDWHKQMIYIPQTPYVFEMSLRDNITFYTPNASDEEVIRAIHMVGLDSLL
                     SELPDGLETRIGNGARPLSGGQAQRIALARAFLDQNRRIMVFDEPTAHLDIETELELK
                     EKMLPLMSDRLVIFATHRLHWLNQMDVIVVMEKGRVAEVGSYQELLAKKGYLYQLKHA
                     MGGNNV"
     gene            complement(29267..30286)
                     /gene="cydB"
                     /locus_tag="VBC52_000724"
     CDS             complement(29267..30286)
                     /gene="cydB"
                     /locus_tag="VBC52_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEO6882325.1"
                     /translation="MSALQFFWFFLIGLLFSGFFFLEGFDFGVGMAVQTLTHNEHEKD
                     QVVETIGPVWDGNEVWLLTGGGAMFASFPYWYASLFSGYYLILLTILFGLIIRGVSFE
                     FRHKVPAEKKQFWNWTLTIGSAIVPFFFGIMFISLIQGMPLDASGNLSAQFSDYFNIF
                     SLVGGVAMVLLAYLHGLNYIALKTEGPIRERARNYAQLLYWVLYLGLALFAVLLYFKT
                     DFFSNHPIVTTIMVLVIVVLAVLAHASTFKGAEMTAFLASGLSLVSVVVLLFQGLFPR
                     VMISSISPKYDLLIQNASSTPYTLKVMSIVAITLVPFVLAYTAWAYYIFRKRITLPVI
                     VTGEK"
     gene            complement(30287..31714)
                     /locus_tag="VBC52_000725"
     CDS             complement(30287..31714)
                     /locus_tag="VBC52_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEO6882326.1"
                     /translation="MTIETLARFQFAMTTVFHFFFVPFTIGTCLVVAIMETMYVITKN
                     EEYKKLTKFWGNIMLLSFAVGVVTGIIQEFQFGMNWSDYSRFVGDIFGAPLAIEALLA
                     FFMESTFLGLWMFTWDNKKISKKLHVTFIWLVVFGSLMSAMWILTANSFMQHPVGYEV
                     VNGRAQMTDFLALVKNPQFFYEFTHVIFGAITMGGTVVAGMSAFRLLKSEQLKDTTVE
                     LYKKSVRIGLVVALLGSISVMGVGDLQMKALIHDQPMKFAAMEGDYEDSGDPAAWSVV
                     AWANEAEHKQVFGIKIPYMLSILSYGKPSGSVKGMDTANKELVAKYGKDNYYPMVNLL
                     FYGFRTMAAMGTAIMGVSVLGLFLTRKKKPILYKHKWMLWIVALTTFAPFLANTFGWI
                     VTEQGRYPWTVYGLFKIKDSVSPNVSVASLFVSNTVYFLLFGGLAVMMISLTIRELKK
                     GPEYEDEHGHHGAYTSIDPFEKGAY"
     gene            complement(31817..33025)
                     /locus_tag="VBC52_000726"
     CDS             complement(31817..33025)
                     /locus_tag="VBC52_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6882327.1"
                     /translation="MKEILVLGAGYAGLKAVRNLQKQSGDFHITLVDMNDYHYEATEL
                     HEVAAGSQPKEKITFPIRDVINTNKVNFMQDEVLRVDAENKTVTVKNNGELHYDYVVV
                     ALGFVSETFGIKGAMENALQMTNISQAENIHNHIVNTMKLYRETKDENLLKLLVCGAG
                     FTGIELAGAMVDERPKYAALAGVKPEQIEIICVEAATRILPMFDDELAQYGVNLIKDL
                     GINLMLGSMIKEIKPGEVVYGTSKEDEELKSITAGTIIWTTGVSGSPVMGESGFDQRR
                     GRVMVNSDLRDPKYDNVYVIGDVSAFMDTESGRPFPTTAQIATRMGAHVAKNLLHQIK
                     GEATEDFSYSPQGTVASVGNTHGLGVVGKTKIKKYPASVMKKIIMNKSLVDMGGLKEL
                     LAKGRFDLYH"
     gene            complement(33038..33937)
                     /locus_tag="VBC52_000727"
     CDS             complement(33038..33937)
                     /locus_tag="VBC52_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEO6882328.1"
                     /translation="MTLPIFLELVEMKAKTASVLPFLIGLCFSAYYYNSVHPVYVGLF
                     FVAMFLFNMFVDIWNNYNDYRNAVDLDYKNDTNIIGRENLSLRQIEVIMASLVITSSM
                     IGLVLVSQVGLPLLWMGLFCFGIGVLYSFGPRPLSSLPLGEVFSGLTMGFMISLICVY
                     LNTYQNFSWDILNLSKIFLISLPNTLWIANLMLANNLCDKEEDEKNHRYTLVHYTGIR
                     GGLLLFAISNSIALLAIVFEFLFGLAPVTVLLSLLLIPFIYKQTKLLWQKQVKRETFV
                     CAVRILALGSATQVLTYLIGIIF"
     gene            complement(34346..34981)
                     /locus_tag="VBC52_000728"
     CDS             complement(34346..34981)
                     /locus_tag="VBC52_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882329.1"
                     /translation="MNNPKERGNSYEKTYYRKKVNNVSTANYLKLGLVSAMFAGGAFV
                     ALGSTQGVSASTFTAPQATPPKAERQLTDAELYERAQKQVLPKYIQGSLSGILNQHST
                     LYKQQNAAATPQVSSPKAERQLTDSELYESAQKQVLPKYIQGSLSGILNQHSTLYQQQ
                     NAAVTPQVSSPKAERQLTDSEIYERAQKQVLPKYIQGSLSGILNQHSTLNV"
BASE COUNT        11768 a         6758 c         5674 g        10818 t
ORIGIN      
        1 tttggtaaca ccaaaacata cacgaacttt tattcatatg tgttaccatt ttacatcttt
       61 catattgaga tatttgatta gcaatacctc cttgactaac aaaaattact aacacatcta
      121 ggggtatacc ctagtttata tttgttcatg attacctcaa gcgagcattc gctaatggta
      181 atgaactatt accacattgt ctgtcttcct taaattatat aaggacttat gtgtcaccgt
      241 tactgtagcg acctacatct agacctaaca taagtgcccc tccaactatt tctaggaaat
      301 gaagaaaact tcaaatcctt gtgactgttt gcaacatcaa tgcattgata acaattttag
      361 tgtatcgcca ttcttatagc gacccatatt aaaaattaac ataagcgcta cctccattaa
      421 ctaaagttag cttgcctacc aatctttctt ttggagacta gtctccattc ttatacctag
      481 agatattgta atagatcatg gataacctcc ttgatttaaa ctttatcttt ataatttaat
      541 tatagtgtaa acgcttccct aagtcaagaa aaaaatattg atgcaacatt tttcttcatt
      601 attaattaca atctagtcaa catacctctt tgtcaaatcc tctttctttg atacaattaa
      661 tctatgatag atttaaaaga ctatggaatt gagatgtggc ctgaagatag gatcgcttct
      721 tttcgtcgca cacttttagg gtggtatgat caagaaaaac gtgatctccc ttggcgaaga
      781 actactaatc catactatat ttgggtttct gaaatcatgt tgcaacaaac acaagttaat
      841 acggtaatcc cttattataa acgttttcta gagtggtttc ctcaaataaa agatttggca
      901 gacgctcctg aagaacaatt actaaaggca tgggagggac taggctatta ttctcgggtg
      961 cgtaatatgc aaaaggctgc acagcaagtg atggttgatt ttggtggcat atttcctcac
     1021 acttatgatg atattgcttc attaaaaggg attggtcctt atactgcagg tgctatcgcc
     1081 agcatctcat tcaacttacc agagcctgct gttgatggta atgtgatgcg tgtcatggct
     1141 cgtttgtttg aagttaatta tgacatcggt gaccctaaaa atcgtaaaat ttttcaagct
     1201 attatggaaa tactaattga ccctgacaga cccggtgatt ttaaccaagc acttatggat
     1261 ctgggtacag acatcgaatc cgctaagaca cctagacctg atgaaagccc tattcgtttc
     1321 tttaatgctg cttatttgaa tggtacttat ggtaaatacc ccataaaaaa tcctaagaaa
     1381 aagcctaaac caatgcgaat tcaggctttt gtcattcgta accaaaatgg ccagtatcta
     1441 ctggagaaaa atactaaagg gagacttcta ggaggatttt ggtcatttcc tattatcgaa
     1501 acgtcacctc tttctcaaca attagattta tttgatgaca atcgatccaa ccccataatc
     1561 tggcaaaccc aaaatgaaac tttccaaaga gaatatcaat taaagcccca atggactgat
     1621 aatcactttc caaatattaa gcacactttt agtcaccaga agtggacaat agaactcata
     1681 gagggggtgg tcaaagcaac agatttacct aatgctcctc acctaaagtg ggtagcaatt
     1741 gaggactttt ctctgtatcc ctttgcaaca ccacaaaaga aaatgctaga gaactactta
     1801 aaacagaaaa atgcttaatc acaagtgaac tatgacggtt cactttttgt ttacaattcc
     1861 tttacgtcgc tgtaaagttt tcctcagatt atcttttaat accctataaa tagtggtcta
     1921 tccctaatgt caaatacatt tgatagaccc gttagcttat catccttata ctaatattat
     1981 gaaaggagat atttatggaa attggtcagc aaattattcg ctaccgtaaa caacaagcgc
     2041 tctcgcaaga agaattagcc gagaaagtct atgtaagccg tcagagcata tctaactggg
     2101 aaaatgacaa aacttaccca gacatccata gcctcttact cctaagtcaa atttttcaag
     2161 tgagcttaga ccaactaatt aaaggagaca ttgagaaaat gaaatatacc atcactcagg
     2221 ttgataaaaa gaattttgaa cgagatacca aagtaatggt aacattgatg atactattaa
     2281 tgatttcaag ttatccatta gtctatttct tagagtggct aggacttggc atttttgttt
     2341 tattatcgat aattactatg acttatgcta atcgtgtgga acgttttaag aaaaaatatg
     2401 atgtgcaaac ttataaggaa atcctagctg tttcaaatgg aaaattatta gacgaaatcg
     2461 aaaaacggga agaaagagca aaacttcctt atcagaaacc ccttattgtc actgtatttt
     2521 tcctcattac tattgccacc ttttttgcaa gtcgttttat ttttacatgg ttgttccatt
     2581 taagttagta gataataaaa taaagctcag cgtgacgctg ggctttattt tatttaactc
     2641 aattcagcaa taatagcttt cagttggtct ttagtatgaa cacctgcaac ttgtttaacc
     2701 acttctccgt cttttttaaa catcaatgtt ggaattgaca taataccaaa ctgacgagca
     2761 gtttccggat tttcatcaac atccattttc aaaattttga gttcatcttc atcgatttct
     2821 tgagataact gttctaaaat tggtgcttgc atacgacatg gaccacacca agttgcccaa
     2881 aaatcaatga gtactaaacc ttctttagtt tcttctacaa atgtagcatc tgttacttct
     2941 aatgccatct tttttctcca tttcatctta atgataagcc tattgtacaa gaatttagtt
     3001 gtttttgaaa ctatttgcta cgaactcgct aaataaataa acaaagccct aaaatcaagc
     3061 caataaggta acctacaata acatccttag gatagtgaac acctgctatc acacggcaaa
     3121 ttgccaatag aaccgataat attaaacaaa ctattccaaa gtaaacataa tatcgtaaca
     3181 aacacatact aatcatcgta gctgaaaaaa catgtctgct aggcatcgac cttccctcag
     3241 tatccttata aattaaaggt ttaatattcc atttttcata tggtcgtgga aaatcaaggc
     3301 gtttcctaat gtaagacaaa gctataaagc caacagcagg gataattaat atccttaacc
     3361 ataattgaaa tgtcatccct tgccaaagag taaatatcag aaaagatggg tagagtatgt
     3421 acatcattcc tgtgactatt tttagtaaaa aatttaaaag aattattaac tgtggttttt
     3481 ttctaaaagg ttgagatagt ttggcataaa attgttcata tgattccata atttacccta
     3541 acgttacaat tgtggcgcca gatccccctg cattttgtgg ggcataagca aaatgcttaa
     3601 cgtgcttatt acgacgaaga tattttgtca ctccctcacg gataacgcct gtaccaatac
     3661 catgaatgat atcaacttgt cccatattgt taagcaatgc ttgatcaata aaattatcta
     3721 actcttgcat agcttcttcg tatcttttac ctctaagatc aagtcgagct cttggtccag
     3781 aactatcagc ctttctaacc acattaatct gtttactttt gactttctgt tcttcttgga
     3841 ctctaacaag ggtaaattca tcttgtgtta atgtcatttt gataattccc acttgtgctt
     3901 cccaacgacc atcttttaat tgactagtta aagtacctcg ctgtccatag ctagtcacta
     3961 taatatcatc accaattcta ggagcacgag ctgctttgat ttttttagcc ttatttaaga
     4021 ctttattttt tgataaatca acttgaggtg ctaatttttt tatttgtgcc ttagcatcta
     4081 taatttcgtg aggttttaat tgactcttat cattgagttt ttttaagata gtatcactct
     4141 cattcaaagc catatctaca atttcttgag cttcttgata gattttttct aactctttat
     4201 cgcgctcatg tgaaaattca ttatagagtt tcttaaccgc acgattgaat ttgaggtttt
     4261 cttgttcaac ttctttaata tgatccagtc ttctacgtgt ctcaagtgtc tgtgcctcta
     4321 actgttcaat aatacggtta acatctgagt cagaatctgt catctgctta gcttgtttaa
     4381 caataaatgg agctaaacca aggcgagaag caatttcaaa tgcatttgat cgtccaggaa
     4441 ctccttgcat aaagcgatac gtagggctaa gcgtttcggc atcaaattcc atgctcgcat
     4501 tctctacaaa atttgtctca atcccataag cttttaattc tggatagtgc gtggtcgcca
     4561 tcgttttgat attacttaac ctaaggtgtt ctaaaatagc catagccaaa ctagcacctt
     4621 cttgaggatc cgttcctgct cccagttcat cgaagagaac taaactattg tggtcagcct
     4681 cgtttaaaat actgactata tgcgtcatat gactagaaaa agttgataga ctttgttcaa
     4741 tagattgctc atcgccaata tctgcaaaga tattgttaaa tactgcaatt ttactacctt
     4801 tatccgctaa tactggcaaa ccagactgtc ccattaattg tgctaaaccg agtgttttta
     4861 gcataatcgt cttaccacca gtattgggac cagtgatgac aattgcagtt aaatcttgat
     4921 caaaatgtaa gtcattagcc acagggttac ttaacagagg atgacgaaca ttgattaatg
     4981 ctaacgtgct gtcattagaa atctcaggta tcgtcgcctt attatcagtc ataaaaagat
     5041 atttagccct tacaaaatca agatgcccaa gaatccatgc attatttcta atagtggcga
     5101 catggggtct taacaagtct gaaaatgcgt gtaaaatacg actttcttca tgacgttcgt
     5161 cagctctaag ctgcgttatc tcttcgttta gtgtaactac agcacgaggc tcaatataaa
     5221 cagtacttcc tgaagaagag atgtcatgaa ccacaccaga aatacgattc cgataagtat
     5281 tttttactgg taggacactt cgtccactac gactagcgat tagattctct gataaaagct
     5341 ctgctttttc cttaagcata tcctgtaaaa tctgacgaac ccgtcgttca ctgtttgtta
     5401 attgacgacg gatacgctct aattctggac tcgcaaaatg ttctaaaaaa ccaccatcat
     5461 tgatagcttg aaaagaccct tgcagattag ggaattgttc caaattttca aacaaacgat
     5521 ccaaagattg gaaagaaaca ttatccaaat cagaataaaa gtgaatcata tccgaagaac
     5581 tctgtaaaac ttttttgata gccaaaagtt cttggatatt aagatcagct gaaagctcta
     5641 agcgttttaa actctcagag attgaactta ggctgactat gccaaatgag tgatgttcaa
     5701 caaaaatctg ttccatgtca gaaatttcat taaaactttt ttctatttta ggagcctccg
     5761 tcatcggctc cagctctgat aattcttctt gtgattgttc tgtcttgaga taaggtaata
     5821 tcaattcctt aactttgtta aattctaact gttctaaaat cttgttattc atactcttca
     5881 ttatacctaa atttatcaca aaatgacaac gttaaaaagg ttggaactct tactccaacc
     5941 ttactaaaca ttatattcaa agacctaccg cccaagtcct taccaaaaac tgagagaaga
     6001 atggtaagtc gttaattaaa aatctaacaa acagactatt ccataacttt tcttgaacaa
     6061 atgacatagg gactgttgcc agaatagtca aacacatact cataaataag agacaaacca
     6121 gaacggctaa aaaaccacta ataatattat tttgcaagct atcaaaatgt tcaactggtg
     6181 ctaaatgtag taaaacacct agcaagcgac ctactaagta ggataaactg tagataaaaa
     6241 caaaggcaac acctgcataa taaacatgac tcaattgaaa tttgctaata tctttaaaaa
     6301 agtaaacttc tactccttgt acaggattag aatacggtac ccataacgta atctgactag
     6361 ctaattcttg gtaaaattga ctagcaatca tcaaagatac cattgacatt aacacgtaaa
     6421 agacttgtaa aaaaatacct ctactgtagc caatataaaa atgccaaata acaataatca
     6481 gcaaaagcaa tgatagcatg attaatcctc actcactttt tctttaatgt ccgaaatcgt
     6541 tttggagcgt aacttattga gctcatcttc cattttttca atgtcaattt ctcgacttag
     6601 ttgcactgat aaagcattaa ttgccatgag aatagcaatc gtttcattat cagcattagg
     6661 taatttttct ttaatagctt catacttttc tgtagctaca cgctcaatct cttccataaa
     6721 aaggttgtct ttatctgtcg tcaatgtgag aggcttatct ccaaagacaa atttataacg
     6781 atttttctga ctattattta tttttgtttc catactatac ctctgctagc attataccgt
     6841 aaaatagggc tttcgtaaaa ttctacccct tatcattact tgtctattcc cttattcctc
     6901 ttttttatga tagaatattg tctatgaaca caatcgtcat gcaagcagat aaaaaattgc
     6961 aagaaaagat aagaaccgat ttagctcaac atcatataag taataataat ccttatgtgg
     7021 tattttcagc taaaatatca ggaactactg ttcttttata tacttctgga aaacttgtct
     7081 tccagggttc taacgcttca catatcgccc aaaagtatgg ttatattgag caaaaagaaa
     7141 gttgttcctc tgagagtcaa gatattccca taattggtac tgatgaagtg ggaaatggct
     7201 cttactttgg tggactggca gtggtagcta gttttgtaac tcctaaggac catgcttacc
     7261 tcaaaaaatt aggtgttggt gattcaaaaa cacttactga tcaaaaaatt aagcaaaccg
     7321 cgcctctttt ggaaaaagca attccacaca aagcattatt gttatcgcct caaaaatata
     7381 atcaagtggt tggtccaaat aacaaacata atgctgtctc cgtaaaagtt gctttacaca
     7441 accaagctat cttccttctt cttcaagatg gttttgaacc agaaaaaatc gttattgatg
     7501 cttttactag ctccaaaaac tatcaaaact atttgaaaaa tgaaaagaat caatttaaac
     7561 aaacgattac tctggaagaa aaagctgaaa ataaatatct agctgtagcg gtcagttcta
     7621 ttattgcacg taatctattc ttagaaaatc ttaacaaatt aagcgacgac gtaggatata
     7681 aacttccaag tggagctggt caccaatctg ataaggtcgc tagccaattg ctcaaagctt
     7741 atggtatctc ttcgcttgaa cactgcgcaa aacttcattt tgccaacacc aaaaaagcac
     7801 aagcattatt aaaataaata gcagggaaaa ttatgaaaga atttattaaa gaatggggta
     7861 tctttatcct catcctctca ctttttttac tatcgcgtat ctttttatgg caattcgtta
     7921 aagttgacgg acactccatg gatccaactt tagctgacaa ggaacagcta gtagttctca
     7981 aacaaacaaa aatcaatcga ttcgatattg tagtggctaa cgaagaagaa ggcggccaaa
     8041 agaaaaaaat tgttaaacgt gtcattggta tgccgggtga tgtcatcaaa tataaaaatg
     8101 acaccttaac tattaacaat aaaaaaacag aagaacctta cctcaaggaa tatactaaat
     8161 tatttaaaaa ggataaatta caggaaaaat attcgtataa cccacttttc caagacctag
     8221 cacaaagctc taccgctttc actactgaca gcaatggcag cagcgaattt actactgtcg
     8281 tgcctaaagg ccactactat cttgttggtg atgaccgaat tgtctctaaa gatagtcgtg
     8341 ccgtcggtcc cttcaaaaaa tcaacgattg tgggagaggt taaattccgc ttctggccaa
     8401 ttcgtcgttt tggaactatc aactaaagga aattaaaaat tcacctgaca aaagttgaaa
     8461 tagcatatta tattgaaaat agggagtgac aaatccattt aaaataggga tttacaatca
     8521 ctccctttaa ctgtttatta aaaaggaagg tacaatggaa gtatttttta caggaaccat
     8581 tgaacgcatt atatttgaaa atgccagcaa tttttttaaa atattacttc tggaaatcga
     8641 agatacggat agcgattttg atgatgtaga agtaattatt actggtacta tggccgacgt
     8701 tatagaggga gaagaatata ctttttgggg aacactaacc caacatccaa aatacggcga
     8761 gcaactacaa agtgtacgct atgaaagagc caagcctact tcaggtggcc ttgtgaaata
     8821 cttttctagc gaacaattta aaggaattgg taaaaagact gcccaacgca tcgtagaact
     8881 ctatggagat aataccattg ataaaatcct tgaatcccct gaacagttaa gcacaatctc
     8941 aggactatca aaaattaata gagaagcatt catcgctaaa ctgaaactca attatggaac
     9001 agagcaagtt ttagctaaac tcgctgaata tgggttaagc aatcgtgctg ctattcaaat
     9061 atttgatcac tataaagaag aatcgcttga agtgataaac gaaaatcctt atcaacttgt
     9121 tgaggatatc caaggtatcg gttttaaaat agctgatcaa cttgctgaac aagtcggtat
     9181 tgaaagtgat tctcctaaac gctttcgtgc tgctattatt catactttag ttgagtcttc
     9241 tatggagcaa ggagatacct atattgaggc tcgtactctc ttggaaaaaa ccattactct
     9301 ccttgaagaa gctcgtcaaa tcgaactaga tccttcaatt gttgctaagg aattgacaaa
     9361 tttaattgca gaagataaag tgcaacatat tggaactaaa atttttagta atactctatt
     9421 ttttgccgaa gaaggcatca aaaaaaactt acaacgaata ctaaatcaac cctttgataa
     9481 acaactaagt cacaaagata ttgaccgtga aatcagagat attcaaaaat ctttaaatat
     9541 ccactacgat aatatccaag aaaaagctat tagagaagct ttactaagta aggtatttat
     9601 cttgactggt ggcccaggaa caggtaaaac aacagttata aatggtatta ttgaagccta
     9661 ttccgagtta catcatattg atttaaacaa aaatgatatt cctattgtac ttgctgctcc
     9721 tacaggtcgt gctgctagac gcatgaatga acttaccggt ttaccgagtg ctactattca
     9781 taggcatttg ggattaaacg gagatagtga ctatcaaagt ctagatgact atctagattg
     9841 ttccttaatt atcattgatg aattttcaat ggttgacact tggcttgcta atcaattatt
     9901 tgacgctttg gactcgcaca cacaagttat tattgtcgga gatagtgatc aattaccgtc
     9961 tgtaggtcca ggacaagttc tagcagatct tttaaatata aatgcacttc cacacgttaa
    10021 acttgaaaag atttttagac aatcagaaga atcaactatt gtgactttag ctaatcaaat
    10081 gagacagggc tttttgccag aggactttac agctaaaaaa gccgatcgct catactttga
    10141 agcatctgct aatatcatcc caaatatgat ttctaaaatt gttcaatctg cacttaagag
    10201 cggtattgaa gctcatgaaa ttcaaatttt agctcctatg tatcgcggcc aagcaggtat
    10261 taataatctt aaccttatca tgcaaaatct cctcaatcca ttaaaagata ataaccaatt
    10321 tacttttaat gatataaatt tccgaatagg agataaagtg ttacatttag tcaacgatac
    10381 agagcttaat gtttttaatg gggatattgg ttacataacg gatcttatac cagccaaata
    10441 tactgagagc aagcaagatg agatttatat gacttttgat ggtcaagaag ttatctacca
    10501 acgcaaagaa tggcttaaaa taacactagc ctatgctatg agtattcata aatcacaggg
    10561 ttctgaattt caagttgtta ttctgccaat aacgagacag agtggtcgaa tgttacagcg
    10621 aaaccttatt tatacagcca ttacgagatc taaaagtaaa ctaatacttt taggagaaat
    10681 tggtgcattt gatttcgctg ttaaaaacga gggggcaaag cgcaatactt atctgataga
    10741 aagatttgaa aacaagcaag aaatagctaa ttctcaaaaa attgaagata gctcaataga
    10801 tcaaaagatt gacagcacaa taattaatac gtccattcca aaaacagcaa cgccaattga
    10861 gcagactaat ctttcaaaaa taacttatcg cctaactgaa gaaaattatt taacaatcga
    10921 tccaatgatt ggtattaatc aacaagatat aagtgcaatt tttgatagta aataattttt
    10981 tcataaaatc ttgaaaactc ttccaaatat gttataatat cattgtatta agaacgtagt
    11041 tcaaatttaa tacttttatt aaggtttatt tagaaaaagg agatattatg gtttcatatg
    11101 aaaaagtccg ccgctctctg cgcacagcta caatcactat cattgtatta aatagtttgt
    11161 ctttagtatt cagattgttt acaggaattt cagtgcagct agccaaaact gaaattaata
    11221 aagggaacac aggtaatctt cctaaagaac atatcgaagc agtcttgagc gccacaacac
    11281 catttatgct atttgtcact gctttaatag tattagtcaa cattgccatt gttattttct
    11341 gtattaaaaa tttaagagca attaaaagaa atcaaatggt taattacctt ccttattacc
    11401 ttggcttcgc cattactgta ggacttgtta ttttgggttt tctaactact aaagcaccat
    11461 gggcaatagc tataaatatc gtcttccaag ctattttcgg tcttctttat ttccatgctt
    11521 atcaaaaagc acaaaaactc aacgaacgtg atcttgaaga gactaactaa aagtaaaagc
    11581 actagaatct agtgcttttt tcttttcaaa gagagttaaa tatagtcatc gtcccaactt
    11641 ttaaaaatct aaatagatag cttcatgttc attttccagg gtcgttactg taacacctaa
    11701 gagtcgtatg ccaaaaactg actcttctaa ggtatcaaat atcgctttag caacttgatc
    11761 aatgatttga aaatcttgag tatactcttc taatgtcata cgtttcgtca atgtttcaaa
    11821 atccgcatat ctaactttta aaacgattgt cttaccaact ttcttatttt tctccagaga
    11881 agcaacaact ctctgaacgt tctttgaaat ctcagctttg atatctgctt cgttatatag
    11941 taatttccca taggttttct cacttcctat tgatttacga actctatcag gttttaccgg
    12001 ggaagcatta atccctcttg ccttacgata aaggtcatac ccgaaacgac caaacatatc
    12061 aattaaagag atttcagata aggaaaggag gtcctcacca gtataaatcc ctaaagcatg
    12121 taatttttcg acggagcgtt taccaacacc atgaaatttc tctattggta agggcttcaa
    12181 gaaatcttga gcttgatctg gtaggatgag ggtaagtcct tttggtttct caaagtcaga
    12241 agccaatttt gccaaaaatt tattgtagga aatacccgct gaacaggtca aatggacatc
    12301 attccaaata tcgtattgta tcattttagc aagtttaaca gcagatttta ttcccatttt
    12361 attttcagtt acatctaaat aagcttcgtc tatactcatc ggttcaacca aatccgtata
    12421 ctttttaaat atatctcgta cttccattcc tacttggcga tatttttggt aatttcccga
    12481 aataaagata gcttgaggac atcgttcata cgcttctttt gaagacatag ctgaatgaac
    12541 tccaaacttt cgagcctcat aattacaagt agaaacaacc cctctgccgc cggtttttct
    12601 gggatcagaa ccaataataa caggctttcc tttaagagag ggattgtcac gctcttccac
    12661 agatgcaaaa aaagcatcca tatcaatgtg gataatttta cgcgacgtat catttattaa
    12721 aggaaaaatt aacattgttc tctcccccat tctctctact ccattataac agattacaac
    12781 ttaatgatac agattaaaaa aactgtatta taaaagaatc aaaattttgt caagaaatat
    12841 gcttgcgaaa acgtttactt ttttctaaaa tggatatgta aacaaaatta aatctgtcac
    12901 aatgtttaag gagaaaacta tggcaacagt taaaacaaac acagatatct ttgaacaagc
    12961 ttgggaaggc tttaaaggtg ttgattggaa agaaaaagca agtattgctc gcttcgttca
    13021 agcaaactat gcaccttacg atggagatga aagcttctta gctggagcaa ctgaacgttc
    13081 acttcacatc aaaaaagtga ttgaagaaac aaaagctcac tatgaagaaa ctcgcttccc
    13141 aatggatact cgtgttgctt caatttctga attaccagct ggttttatcg acaaagataa
    13201 cgaacttatc tttggtattc aaaacgacga gcttttcaaa ttgaacttca tgcctaaagg
    13261 tggtatccgt atggcggaaa ctacacttaa agaaaatggt tatgaacctg atccagctgt
    13321 tcacgaaatc ttcactaagt acgctacaac agtaaatgat ggtatcttcc gcgcttatac
    13381 ttctaacatt cgtcgtgctc gccatgcaca cactgttact ggtcttccag acgcttattc
    13441 acgcggacgt atcattggtg tatatgctcg tctagctgtt tacggtgctg actacttgat
    13501 gcaagaaaaa gttaatgact ggaatgcatt gaatgatatt gatgaagagt caattcgtct
    13561 tcgtgaagaa atcaaccttc aataccaagc tcttggtgaa gttgtaaaac ttggtgacct
    13621 ttacggcgta gatgttcgta aaccagccat gaacactaaa gaagccattc aatgggtgaa
    13681 tattgctttc atggctgtat gtcgtgttat caatggtgct gcaacatcac ttggacgtgt
    13741 tcctattgtc cttgatatct ttgcagaacg tgacctcgct cgcggtacat tcacagaatc
    13801 agaaatccaa gaatttgttg atgattttgt ccttaaactc cgtactgtaa aatttgctcg
    13861 tactaaagct tatgatgccc tttactcagg tgacccaacc tttatcacaa cttcaatggc
    13921 aggtatggga gctgatggac gtcatcgtgt taccaaaatg gactatcgtt tcttaaatac
    13981 acttgacaat atcggtaact caccagaacc aaacttaact gttctttggt cagaccaatt
    14041 accatatgct ttccgtcgct actgtatgtc aatgagtcat aaacactctt caatccaata
    14101 tgaaggtgta tcaacaatgg ctaaagaagg ctacggtgaa atgtcatgta tttcatgttg
    14161 tgtatcacca cttgatccag aaaacgaaga caaacgtcac aaccttcaat attttggtgc
    14221 tcgtgttaac gttatgaaag cacttcttac tggtcttaac ggtggttatg atgatgttca
    14281 taaagattac aaagtatttg acattgaccc aattcgtgac gaagtactca actttgacac
    14341 tgtaaaagct aactttgaaa aatctcttga ctggttgact gatacttatg ttgatgcgat
    14401 gaatatcatc cactacatga ctgataaata caactatgaa gctgtacaaa tggcattctt
    14461 accttcacat gtacgtgcta atatgggatt tggtatctgt ggttttgcaa atactgtcga
    14521 ttcattatct gcgattaaat acgctactgt taaaccaatc cgtgacgaag atggttacat
    14581 ctacgactac gaaacagttg gtgacttccc acgttatggt gaagatgatg atcgtgttga
    14641 ctcaatcgct gaatggttac ttgaagcctt ccatggacgc cttgctaaac acaaacttta
    14701 taaagatgct gaagcaactg tttctcttct tacaatcaca tcaaacgttg cttactctaa
    14761 acaaactggt aactcaccgg tacataaagg agtatatctc aatgaagatg gtagtgtaaa
    14821 cctttctaaa gtagaattct tctcaccagg tgctaaccct tctaacaaag ctaaaggtgg
    14881 ctggttacaa aacttgaatt cactttctaa acttgatttc gctcatgcaa atgatggtat
    14941 ttcattgaca actcaagttt ctccacgcgc tcttggtaaa acatttgatg aacaagttga
    15001 taacttggta acagtccttg atggttactt tgaaaacggt ggtcaacacg ttaacttgaa
    15061 tgtcatggac cttaaagatg tctatgacaa gatcatgaat ggtgaagacg ttatcgttcg
    15121 tatctcaggt tactgtgtca atactaaata tctaactcct gagcaaaaaa ctgagttaac
    15181 acaacgtgtc ttccatgaag tactttcaat ggacgatgca ttaactaact agtttgtatt
    15241 cgatgaagta ggtttgatat aatcaaacct acttttatct atattatcaa tagtactaat
    15301 tacttcttta tataacctca aggagggcct aatgttaaat cacaaatttc ttcaagttct
    15361 aaaatatgag ggtgtcgttt cgataacaag ctggaccgaa ttagctcctc acgttacaaa
    15421 cacttggaat agttacctga ctattacaga tgaccagcgt atattagctc ctgcagcagg
    15481 aatgactcat cttgaaaatg atctcaataa taattctaac attattatga ctctgggcag
    15541 ccgtgaagtt gagggtcgtg atggttatca aggtacaggc tttagaattg aaggaactgc
    15601 taagctatta gaagcaggaa gcgattttga aattgttaaa gaaaaatatc cttttctgcg
    15661 taaagtattg gaagtaactc ccataaatgt tatacaactt ttataaaaaa cttctcctag
    15721 aaaattctag gagaagtttt ttattcaaag cacgatgtta tcgcctcttg tatatgtcgt
    15781 cgtttctcaa tccaaagtgg acggtcatca taggaaaatg ttctgtttgt caaaaaaata
    15841 gctgctgcct gcttttgata atttagtgca ataaaaggac cagtataacc agtatggttt
    15901 atccaatcct tatctatatc ccaagctaat gatcttgatt tactcaaact ataattattc
    15961 cacaaaggtt tcgcaaaatc atcttttaaa taatgaatag aaaatctttg taaatcatca
    16021 atagttgaaa ataaacctgc agaaccagta tgctttccta ataccttagc tttaggatcg
    16081 tgtacaatac catcgttaat accggctaca gttggaacag cttctactct aggaccaaaa
    16141 gaggtttctt tcatctgaaa aggagtgaaa atataacgtt tgaaaagttt atccaaacca
    16201 tcccctaata cctcttctaa cataaaacca agtaaaagaa aattaatatc agtgtattta
    16261 aacgatttat cttccaaaac cttgatatga ttaatggcat ctttcaactg agtagcattc
    16321 aactgatcac gatttggaat aaaaggatca atcccactgc tatgtgtagc tagttgcctt
    16381 acagttaacg tctgatgatg aaacctaggg taataatacc ttaatggctt atcaatatca
    16441 atagtcccgt gataaactaa ctttgctagt aaagttccca cgccgacaac ttttgataca
    16501 ctggctagat catagactaa tcccgcttta actttctcat tgccatcaat tgtccccaag
    16561 taatgttcgt gccactctcc attttcataa atagctaagc ttgcaccatg ataaagatta
    16621 tctttgattt gctctttaat gagttctaaa acggtattag ttttaatcat tcttttgaaa
    16681 tccaaatctc aatattacta gtgtcagaga tgactaaaat cttttcttta gaatcaaggt
    16741 aaacatcaag tcccaatgat tcaaaagttg atttcaagtc atttaaatta gtatcttcat
    16801 taacacagca ttctagtatc tcaatatccc aagtttcatt tggagcaatt tgcaaatctt
    16861 gtccttgtgc ttctttgaaa gataaattaa ttgggaactt cccagcaaat actttatcgt
    16921 aaaaagcttc agctttcacg gcatcaggta cattcaaact aatagattgg aaaacaaagt
    16981 ttgatagacc tgtagtgtca tcattttttt ctaactctgg gcgctcaatt gctactaatt
    17041 gtgacaaatc atcttcagca tgaagtaaga aaagatctcc ctcgggcgaa actgtttcat
    17101 aggcataacc attttgacct tgatatactt gaatggcttg cgcaccattt gccaaaagct
    17161 tttcaatatc ttttgcatct tgagacttaa caataatctt tgctaacttc ttagtaccat
    17221 tcaccgcacg agtacgataa gttggggatt cctcaataat aaagctagcc tctttgttct
    17281 gccatgctga gaaaactgcg attgcatttt cttcacttat caatttaaat ccaagagact
    17341 cctgataaaa tgtaatattc aagtctctat tattaacacg taatactggg tgtttaaatg
    17401 tcaatgaatg aaataacgtc ataaattcct ccttgttcag tctagtctat tttagataat
    17461 tttagataat ttagcaagaa tctttgtgct ctcaatacca taaagtcaaa ttgacagttt
    17521 cactattttt ttgtagactg gatattatga atgaaacact actattacat gggattcagc
    17581 ttattctgat tatcgccatg attatcactt tttatcagat tgttagacat attaggtctc
    17641 aaaaaataaa tcctttcaaa cgatttttca ctggtttgtg gatcggtttt gtaactgatg
    17701 ctctcgatac tcttggtatc ggctcttttg caacaacgac cacatttttt aaactaacta
    17761 agcttgttga agacgatcgt aagatacccg caacaatgac agctgcacac gtacttccag
    17821 ttttgttgca gtcactctgt tttatttttg ttgtaaaagt agaggcttta acactaataa
    17881 caatggctgg tgcagctttt attggggctt ttgttggagc taaaatgact aaaaattggc
    17941 acgctcctac tgttcaacgt attctaggaa cactgctcat cacagctgct attattatgc
    18001 tctatcgtat gatcactaac cctggagctg gtatttcaga ttcagtgcat ggtctacacg
    18061 gcatttggtt atttgttggt atcgtcttca attttattat tggtgtatta atgactatgg
    18121 gactaggtaa ttatgctcct gaacttatct tcttctccct aatgggactg agtcctgcag
    18181 ttgctatgcc tgtcatgatg ctagatgcag ctatgataat gacagcttct agtacacaat
    18241 ttatcaagag tggtcgtgtt aattggaacg gctttgccgg tctggttact gggggaatat
    18301 taggcgttat tgtcgctgtt ctcttcttaa ctaaccttga ccttaatagt ttgaagacac
    18361 tggttgtagg tattgtttta tttactggag caatgttaat acgttcttcc tttaaaaaaa
    18421 ctatgcccaa aagtcatcat taaacaaaat cagctagcaa gactggctga tttttatatt
    18481 taaaacaaaa aagaccggta aaccgatctt tctttaacta ttaaagatat aatagtttga
    18541 acaaggctac agcgagtata cttgctacga ttggtgctaa aactggcacc catgcatacc
    18601 accattttga atcttcttta gcttgtccaa gaatttgttt tggtaataag cggtgaacaa
    18661 tacgtggacc aaggtcacgc gcagggttca atgcaggtcc agtcggtcca cctaaagaag
    18721 caactagagt cattaccaag aaaccaatac ctaagtgagc tacagcaagt gaaccagtta
    18781 cgtatggtga aatccttgtt gctgctgtcg tttggtcata accagcttga acaagtttac
    18841 caacaagttc tacaccaaaa tagttcttcg ttaaagccaa agctccaaag aaaagaacaa
    18901 aagaaccaac gaattcattt aagaaaccat taatgtaagc cgctttacga cttgatttct
    18961 gtccatcatc taatgcagaa atagttgaaa atgatccaag aacatgattt ggattttctg
    19021 tcttaacaaa gtaaggttga taaaccataa cgacaactaa ctgtccaaac attgctccca
    19081 aaatttgtgc aagaatgtat tgtccaacgt gtgcccaagg gaaaagacct gaaaatgcca
    19141 atccaagtgt gaaagctggg ttaatatggt ttccagagac attaccaaac ataagcgctg
    19201 gcatcataac acctaaacca taaccaattg caataataat ccaacctgaa ttatttcctt
    19261 ttgtgccttt taagtcaacg ttagctacag ccccatttcc taagatgata agaaaagcgg
    19321 ttgcgataaa ttctgtgata tacttcacag tccaagtgat ttcgatcaat ccttgtttcc
    19381 tcccaaaaaa atatttacaa cattaaccat tctaccaagt aagcttaata ttgtaaagct
    19441 tatcacaaaa taaaattaat aaaattttta accaaatata tttgataaaa aaatagagta
    19501 ctaggttcgg actacctagt actctatttt atttagttta taaggttttc ttactatcac
    19561 gaacgactag catatcaact ttcgaatgac gtaaaatgta ttctgaagtt gagccgatca
    19621 atagtctttc aaaagtgttt aatcctgttg cgcctaccat aattaaatct gctttttccc
    19681 ttatggggat atcctttgcc aaaagagtct taggatttcc gatttcagta atttttttga
    19741 tctttgtaag gccttttgaa tgtgctaatt tctcaaaata ggctagcaca tctgctgact
    19801 cttgttcttg cctatcaaaa acatagtcac taaagactcc ctcactttgg taagcgtgag
    19861 catctataac atgcgttagc agtaattcag catcattacg aagagcaaca tttatccctt
    19921 tttctaccgc caattctgac tcgtaagacc catcgatagc aataagaata cgctcatatt
    19981 tttgtgtcat agcttaattc ctccctttta tatagtttta gtttcaactt gtctagatga
    20041 ttttaagaaa tatagaatac cacaaacaac aggaataatc gctgctatcc agaataattc
    20101 tctaaaggat tgcacaccag ctccaagaaa accatcttta acaagtccca aaatgtaagg
    20161 tccagcacct aaccctaaat caagacctat catgtaagtt gacatggctg tattgaaacg
    20221 atgttcctca acacctttga ttgatgctgc ttggccacaa gacataaagg taccataacc
    20281 aaaaccaatc aaagcacctg atagaaggta ggtaacactt cccatagcac tccctaataa
    20341 agcaagtccc aaagttaaga acagataact tggataaagc acccacttat ctcccttagc
    20401 gtccattaat cttcccatag atggtctggt taaagtgatg acaagtgcat aaacaataaa
    20461 gaaataagct cctactgtca tcaaattaat ttctgttgta tataattttt ggaaacctaa
    20521 cacggaagca taggagatac ccatcaaaaa tgcaataatt gtgataaaaa ttgctttttt
    20581 ctcaatgaaa ctatcaatag tccatgattt tgatttagct aactgttctg gatttaaagt
    20641 aatatttttg actgggaaaa caaatgctcc caacactaca atcgcaatta aaatactaca
    20701 taatacaata accattttaa agttaatatg aaggttgtct agcataaatg ttcctacaaa
    20761 aggaccaata gctgcggcta aacttgttga tagaccgtaa aagttaatcc cctcacctct
    20821 tttatcagct ggtatataag ctgttacaat agtattagtt gctgttgaca cgacgccata
    20881 accaaaacca tttaggaaac gaactaaata cattactccg atacttggca tataaaaata
    20941 agctaaagtt gttagtaagt aaaaaatagc ccctccacgt aaaactaact tacgtcctag
    21001 aacttctaat tgcttgccaa atataagacg agcaatcaaa gtccctacaa tataaatccc
    21061 cgtcgctaat cctgcttggc tagtgctaac acctaactct ttagtcgcaa taaaagctat
    21121 gataacggta aacaaataat agaccatata aacaataaag tttaagatag taatgcctat
    21181 aaaatgtttg ttgaataatt tgtcttccaa tgcaaatggc tccttttttc aatttcattt
    21241 tcataaacat tttacaaaaa tattagaaga tacgatattt caatggtata atataagtgc
    21301 tagtatcaga ctgcattaac aaatgttaag gtggtctatc aaacctaaaa catttggagg
    21361 aagtcatgaa tcatcaaatt ttacaaaatt acattaatag tcataatctt ccaattatcg
    21421 aaaaagacta ccataaatac cttacatttg aaagtttaga agaagactat acttatatcc
    21481 taaaagatgg tattgttaaa caaagtgttc tctctaaata tggtactgag ttcaatctgc
    21541 gttatgtgac aggcttagaa atcacttcta tcctaaatac agactactcc caacacatgg
    21601 gagaacctta caacgttcga atcgaatccg aaactgccca cttttacaaa gtacgtcgct
    21661 ctaccttcct aaaagatatc aataatgata ttgaactgca aggatatgtg aaagatttct
    21721 atcataaccg ccttgaaaaa tctatgaaaa aaatgcaatg tatgctcact aatggtcgaa
    21781 ttggtgctat ctccacacaa ttatatgacc tcagtaagat gtttggtgaa gaacgagata
    21841 acggtgatat ctatattaat tttgttatca cgaacgaaga acttggtaaa ttttgcggta
    21901 tatccacagg aagtagcgtg agccgcattt tgaaacaact aaaagacgat catattattc
    21961 gtattgaaaa acaacatatc attattacaa atgttgagaa attaaaagat catattgtat
    22021 tttaatataa caatagaaag gatatattat gcgttataat caattcagct acattccaac
    22081 taaacctaac gaagcttttg aagagctaaa aggactaggt tttccattaa acaaaaagaa
    22141 ttctgataaa gctaatttgg aagcttttct cagacattct tttttaaatc aaactgatac
    22201 tgattacgct cttagtctcc ttatcgttga tgcaaaaacc gatgctctaa ccttttttaa
    22261 atcaaatagt gacttaacac tagaaaattt acaatggatt tatttacagt tattaggctt
    22321 tgtccccttt gtagacttta aagaccctaa agctttctta caagatatta acttcccagt
    22381 ctcatatgac aatatttttc aaagtctaca tcacttactc gcctgtcgtg gaaaatctgg
    22441 caatacatta atcgaccaat tagttgctga tggtttactt catgcagata atcactacca
    22501 ttttttcaat gggaagtctc tggccacttt caatactaac caattgattc gcgaagttgt
    22561 ctatgttgaa acatccttag atactatgtc tagtggtgaa catgatttag taaaagttaa
    22621 cattatcaga cccactaccg agcatactat ccccacgatg atgacagcta gcccctatca
    22681 tcaaggtatc aatgatcctg ccgcagacca aaaaacatac caaatggagg gtgcgctagc
    22741 agttaaacag cctaaacaca tacaagttga cacaaaacca tttaaagaag aagtaaaaca
    22801 tccttcaaaa ttacccatca gccctgcaac tgaaagcttc acacacattg acagttatag
    22861 tctcaatgac tattttcttt ctcgtggttt tgctaatata tacgtttcag gtgtgggtac
    22921 tgctggctct acgggtttca tgaccagtgg ggattaccaa caaatacaaa gctttaaagc
    22981 agtcattgat tggttaaatg gtaaggttac tgcattcaca agtcataaac gagataaaca
    23041 agtcaaggct gattggtcaa atggccttgt agcaaccaca ggtaaatctt atctcggtac
    23101 catgtcaact ggtttagcaa caactggcgt tgaggggctg aaagtcatta tcgctgaagc
    23161 cgcaatctcc acatggtatg attattatcg agaaaatggg cttgtgtgta gtccaggcgg
    23221 ctaccccggt gaagatttag acgttttaac agaattaaca tactcacgaa acctcttagc
    23281 tggtgattac atcaaaaaca acgattgcta tcaagcattg ttaaatgaac aatcaaaagc
    23341 aattgaccgt caaagtgggg attacaacca atactggcat gaccgtaatt acctaactca
    23401 cgtcaataat gtcaaaagtc gagtagttta cactcatgga ctacaggatt ggaatgttaa
    23461 gccaagacat gtctacaaaa ttttcaatgc attgcctcaa accatcaaaa aacacctttt
    23521 tttacatcaa ggtcaacatg tgtatatgca taattggcag tcgattgatt ttcgtgaaag
    23581 catgaatgcc ttactaagcc aagaactact tggcattgac aatcatttcc aattagaaga
    23641 ggtcatttgg caagataata ctactgagca aacttggcaa gttttagatg ctttcggagg
    23701 aaaccatcaa gagcaaattg gcttaggtga tagtaaaaaa cttattgata accattatga
    23761 caaagaagcc tttgatactt attgtaaaga cttcaatgtg ttcaaaaatg atcttttcaa
    23821 gggaaataat aaaaccaatc aaatcactat taatcttcct ctaaagaaaa attatctcct
    23881 gaatggacag tgcaaactcc atctacgtgt taaaactagt gacaaaaagg ccattttatc
    23941 agcccaaatc ttagactatg gtcctaaaaa acgattcaaa gatacaccaa ccatcaaatt
    24001 cttaaacagc cttgataatg gtaaaaattt tgccagagaa gctttacgtg aactcccgtt
    24061 tactaaagat cattatcgtg tcatcagtaa aggtgtcttg aaccttcaaa atcgtacaga
    24121 cttacttaca attgaggcta tcgatccaga acaatggttt gatatcgagt ttagcctcca
    24181 accaagtata tatcaattga gtaaaggtga taatctaagg attatccttt atacaactga
    24241 ttttgaacat accattcgag ataatgctag ttactctata acagtagatt tgagtcaatc
    24301 ttatttaact atcccaacta atcaaggaaa ttaacttatg aaacttctca ctaaagaact
    24361 gtttgatgat tctcaacact tttggtacca gatcaattta tcacaagaga gtaacttcgg
    24421 agcagttttt gaccatgata ataaaaacat tccacaggtt gttgcaacta ttgttgatga
    24481 tttacaaggt tccggaagtt cgaatcattt ctggtatttt ggcaatacta ctgatacttc
    24541 catccttatg attgctcatt taaatcgaaa attctatatt caggttaatt taaaggactt
    24601 tgactttgca ctcaatttaa taactataaa taattggaag agtctcctcc aaactcaact
    24661 cgaagctcta aacgataccc tagcaatatt tcaataaata aggtagaatg gagtgacaaa
    24721 gcaacgcgag ggagactgat taatgtcatc ttattggaat aactatcctg aacttaaaaa
    24781 aaatattgat gaaaccaatc aactaattca agaaagaata caggtcagaa ataaagatat
    24841 tgaagcggcg ctaagccaac tcacagctgc gggaggaaaa cagctcagac cagcattctt
    24901 ttaccttttt tctcaacttg gtaataagga gaatcaagat actcagcaac taaagaaaat
    24961 cgctgcttct ttagaaatcc ttcacgttgc tacattaatc catgatgatg tcattgatga
    25021 ctcaccacta agacgtggaa atatgaccat tcaaagcaag tttggcaaag acatcgcagt
    25081 ttatactggg gatttacttt tcacagtctt tttcgatctt attttagaat ctatggctga
    25141 tacaccattt atgacgatta atgcaaaatc tatgcgtaaa attctcatgg gagaattgga
    25201 ccagatgcac cttcgttaca atcaacaaca aggtatccat cactatttac gtgcgatttc
    25261 aggtaagaca gccgaactct ttaaattagc tagcaaagaa ggagcttact ttggtggtgc
    25321 agagaaggag gttgttcgtc tagcaggcca tatcggcttt aatattggta tgacattcca
    25381 aattttggat gatattctgg attatactgc agataaaaaa acatttaaca agcctgtctt
    25441 agaagattta gcacaaggcg tttacagcct tcctctactt cttgccattg aagaaaatcc
    25501 tgatattttc aaacctattt tagataaaaa aacagatatg gctactgaag acatggaaaa
    25561 aattgcttat ctcgtcgttt cccatagagg tgttgacaaa gctcgccatc tagctcgtaa
    25621 atttactgaa aaagctatta gtgacataaa taagctaccc cagagctctg caaaaaaaca
    25681 gttgctacaa ttaactaatt accttttaaa acgcaaaatt taaataataa aaaaacattc
    25741 cacaatgcta gaaaagcagt tagggaatgt ttttttacta tcatttattt atcgcaccta
    25801 tcaattatca tagatcacca tcatcagcgg ctttcagctg acggtaacgt tggctacttt
    25861 gagacaattc ttgaggagaa ccttccaact ctaattgccc attttctata aataagatac
    25921 gatcagcatg ttcaatacct tttaagtgat gtgtaatcca aactaaggta ttaccttcca
    25981 attctttcat aaataccctt agtaaggctt gttcagtaat aggatcaagt ccaacagttg
    26041 gctcatctaa gataacaatt gggacatctt ttagtaagat tctagccaaa gcaattctat
    26101 gcctttcgcc acctgaaaac ctaagtccag cttcatcaac cattgtatag agaccatctg
    26161 ataaatcagt gaccatctct ttcaatccaa ctcgttcaag aactttccat acatcttctt
    26221 cactagcatc ttggtttcca atgcgaatgt tatttagcag ggttgtatta aaaaggtagg
    26281 gcgcttgttg tatcactcca atatagttag aaatgcaatc accaactatt gaaacatcag
    26341 caccgcctag ggtaatctcc ccttgacttg ctttcaagtc gccacgaagt agactagcta
    26401 aggtactctt gccagaacca ctacgcccta aaatagcaat tttttctcct tctttaatat
    26461 ccaaatctaa atgatgcaaa acccatttct cttgtggctt atactggaaa cttaaattct
    26521 tgacggaaaa atcatatggc ttattaggca attgattttg agttgtttca aaatacgttt
    26581 ctgataattc attaagacga tgaattgaat cagaatattt atttgtttct tgagcagcag
    26641 cagacaaacc agcaaatgcc tctgacaagg gaaatactgt tagaacaaat gctgcaatcc
    26701 agttagcttc gccacctctg tgaccaataa actgattact agcccaaata ataaccaaaa
    26761 tagctagaaa gccaaaaact aattccacaa taagagctct acgattatca aaggaacgaa
    26821 tttttttctg aacagccatg agttcacttt ccgaacgctc atgcaaggcg acgtactctt
    26881 gaccacgttg actaaaaatc cagtctgaaa tacctaagac attatccgtc aaatcagtat
    26941 aaagatgatt ttttaattct ttttcacgag tctgacgagc accattagct aaaatcgacc
    27001 aaagtggaaa aacaaaaatc ataatagcta aataaagcaa catcattaaa gcgaaccata
    27061 gagaaaagaa gcctgttgca ataataataa agctatataa taacccccct ataatcgttg
    27121 gaaatattgt cctaagatac aaattttgca aataattaat atcctccgct aaaagcccca
    27181 taacatctcc taaacgaaaa tcacgcttca taaagatagc attgctttct aaggaatggt
    27241 aaagcttcaa gcgcagttgt gaagtcattc gtaataccca attgtgactc gttaggcgtt
    27301 ctatataacg aaaaacagga cgtccaatac caaaagcacg tgttagtaca attggaacat
    27361 acactagaag aatattactc ggtagtgaag ctgacttact aatcagatag cccgaattaa
    27421 acataagagc actggcagaa aagaaagtca taaaaccaag gaaaagagca attactaagg
    27481 aaactttata ttgtttgaaa aatggtttta cccattgatc tgtctttaat tctttaaaca
    27541 aagggatttt aaacattatt tcctcccata gcatgcttca gttgatagag atatcctttt
    27601 ttagctaata attcctgata agagcctact tctgcaactc tccccttctc cataacgact
    27661 ataacatcca tttggttcaa ccagtgaaga cgatgagttg caaagataac taaacgatct
    27721 gacatcagtg gcaacatttt ctcttttaat tctaactctg tttcaatatc taaatgagca
    27781 gttggttcat caaaaaccat aatacgacga ttttgatcaa gaaaagcacg agccagagct
    27841 atacgctggg cttgtcctcc actaagtgga cgcgctccat taccaatacg cgtttctaaa
    27901 ccatcgggta actcgcttag taagctatct aaacctacca tatgaatcgc tcttatcacc
    27961 tcttcgtcac tagcattggg agtatagaag gtaatattat cacgtaatga catttcaaaa
    28021 acataaggag tttgaggaat atagatcatc tgtttatgcc aatcttcttg atcaaggtta
    28081 accacttctc taccatccac ttttaaagaa cctttatcag gccctaaaaa accactcaga
    28141 aggttaatta agctagactt acctgaacca ctaactccta ctaaagcaac cttttgatgt
    28201 cctctaaatc gtaaggttac atcctcaaca acacgttttt ggtcgtacgc aatagcaatg
    28261 ttttctaaat ccaaatgact ctctttagac caatcatcta tgactaattg ctcaccttta
    28321 ataccagttt tgtttagaac tttttgaatc gcttgaaatg catttttccc gtctaacgtt
    28381 gcatgataat cactagaaaa atctcttacg ggtaaaaagt attcagggga taaaatcaaa
    28441 attgttaaag ccggtaaaag gtaaatctgc tcatttaata atcgtaaacc tagaaaaaca
    28501 gcaacgatag caatggataa agttgtaaaa aagtctaaag caaacgttga taaaatacca
    28561 attttcaaag tactcattgt tgctttacga aaagattcac tagtttgata aatgctttta
    28621 ccataacgtt tactaagacc aaaatatttg agagtatcaa tgcctcgtaa tgaatctaaa
    28681 aaatgattag aaagtacttg ataggattca tattgtttat ctgctttagc ttgagccgca
    28741 tagcctaaaa taatcataaa gatgataatt aaaggaaaaa cgataagtaa aattgcacct
    28801 gattcaatat ctaaatagaa aatataagct agaataatcc atgggataat agacatgtta
    28861 atcatcttat tcaagactaa acgtaaataa ttttcaacta aagaaacgcc atctaatgcc
    28921 attgtaacaa cattaccagt tccttgttct tgaacgactt taggacctag atcaaataac
    28981 ttatctaata attgacgtct caaaagacta gattgtgctg ttgaaaagtc atcaagtttt
    29041 ctatttttga tgtaatcaat aacatgacgg cctaaatatg agaccataaa taacaaaata
    29101 taaaccgttt gagaagaaag tttttgccct tcccacaaac cagttataga aagactcaaa
    29161 taataagctt gtccgataat aaagattgct tgaagaacat caagtcccgc aagcaatcct
    29221 aacaacttat gaatacctga caagcgcata accgctttat ctagcattat ttttctcctg
    29281 tcacaatcac tggcaaggta atacgttttc taaagatata atatgcccat gctgtataag
    29341 ctagaacaaa gggaactaga gtaattgcga caatcgacat tacttttaaa gtatatggcg
    29401 ttgaggatgc attttgaatc agcaaatcat atttagggct aattgatgaa atcataactc
    29461 gtgggaatag accttggaac aacagaacaa ccactgaaac aagactgaga ccactcgcta
    29521 aaaatgctgt catctctgct cctttaaaag ttgaagcatg cgctaataca gctaatacaa
    29581 caataaccaa gaccataatt gtcgtaacta tcggatgatt gctaaagaaa tctgtcttga
    29641 aatacaagag aactgcaaac aaggctaaac caagatagag tacccaataa agcaactgag
    29701 cataattacg ggcacgctct ctaatcggcc cttctgtctt caatgcaatg tagtttaaac
    29761 catgaagata ggctagcaat accattgcaa ctccaccaac caacgagaat atattaaagt
    29821 aatcactgaa ttgagctgat aagttaccac tggcatcaag tggcatacct tgaatcaaac
    29881 ttataaacat aatcccaaag aagaatggaa caatagcgga accaattgtt aaggtccagt
    29941 tccaaaactg ctttttctct gcaggaactt tatgtctaaa ttcgaaggaa acaccacgaa
    30001 taattaatcc aaagagaatc gtaaggagaa tcaaatagta accactaaat agtgaagcat
    30061 accagtatgg aaaagaagca aacattgctc caccaccggt aagaagccaa acttcattac
    30121 catcccaaac tgggccaatg gtttctacta cttgatcttt ttcatgctca ttatgtgtta
    30181 aagtttgaac agccatccca accccaaagt cgaatccttc aaggaagaag aatcctgaaa
    30241 ataggaggcc gattaggaaa aaccaaaaga attgtaaagc actcatttag tatgctcctt
    30301 tctcaaaggg atcaattgaa gtatatgcac catgatgtcc atgttcatct tcatactctg
    30361 gccctttctt caattctctt attgttaggc ttatcatcat caccgctaaa ccaccaaaca
    30421 acaagaagta cacagtattg gaaacaaata atgatgcaac tgaaacgttt ggagagacag
    30481 agtccttaat cttgaaaaga ccatagacag tccaagggta tcgtccttgt tctgtcacaa
    30541 tccaaccaaa tgtattggct aagaatggtg caaaggttgt taaagcaaca atccaaagca
    30601 tccatttgtg tttataaaga attggttttt tcttacgagt taaaaagagg cctaaaacag
    30661 aaacacccat aatagctgtt cccatcgccg ccattgtacg gaaaccatag aaaagtaggt
    30721 taaccattgg gtagtagtta tctttaccat acttagcaac caattcttta ttagctgtat
    30781 ccataccttt cacagaacca gatggttttc cgtaagataa aatactcaac atatagggaa
    30841 tcttgatccc aaaaacttgt ttgtgttctg cttcatttgc ccaagcaact acactccaag
    30901 ctgcaggatc acccgaatct tcataatcac cttccatagc agcaaatttc attggttgat
    30961 cgtgaatcaa ggctttcatt tgtaaatccc caactcccat aactgaaatt gaaccaagaa
    31021 gtgctactac taaaccaata cgaactgatt tcttataaag ttcaaccgtt gtatctttca
    31081 gctgttctga ttttaagagg cggaaggctg acataccagc aaccactgta ccgcccatcg
    31141 taatggcacc aaaaataaca tgggtaaatt catagaagaa ctgtggattt tttacaagtg
    31201 caagaaaatc agtcatttgc gctctaccgt ttacaacttc ataaccaaca ggatgttgca
    31261 tgaagctatt agcagtaaga atccacatcg ctgacattaa tgagccaaaa accactagcc
    31321 aaatgaatgt cacatgtaat tttttactaa ttttcttgtt atcccatgta aacatccata
    31381 aaccaaggaa agtagattcc ataaagaaag ctaataaagc ttcaatagcc aatggagctc
    31441 caaaaatatc acctacgaaa cgtgaatagt ctgaccagtt cattccaaat tggaactctt
    31501 gaatgatacc tgtcacaaca ccaactgcaa aactaaggag catgatatta ccccaaaatt
    31561 ttgtcaattt tttatactct tcattttttg tgataacata cattgtctcc ataatagcaa
    31621 ccactaggca tgtaccaatt gtaaatggta caaagaaaaa gtggaaaact gtagtcatcg
    31681 caaattgaaa acgagctaaa gtttcaattg tcatacaaaa cctcttattt tgttttaatc
    31741 atttctttta tagaaatatc tgcaagagct agtttcctag cttcgtaaca attggtaaat
    31801 aacttacttt aatatcttaa tgatataaat caaaacgtcc cttagctaga agttctttaa
    31861 gtccacccat atcaacaagt gatttgttca tgataatctt cttcataacc gatgcagggt
    31921 attttttaat tttagttttt ccaacaacac caagtccatg cgtatttcct actgaagcaa
    31981 ctgtgccttg cggactatac gaaaagtctt ctgtggcttc acctttgatt tgatgtaaaa
    32041 ggtttttagc aacatgagct cccatacgag ttgcaatttg agctgttgtt gggaatgggc
    32101 gaccagactc agtgtccata aatgcagaca catcaccgat aacataaaca ttatcatatt
    32161 ttggatcacg caaatcactg ttgaccatta cacgtccacg acgttgatca aatcctgatt
    32221 ctcccataac cggacttcca ctaacacctg ttgtccaaat gatagtacct gcggtaatag
    32281 atttgagttc ttcatcctct ttactagtac cataaacaac ttcacccggt ttgatttcct
    32341 taatcatact tccaagcatc aaattaatac ctaaatcctt aattaagttg acaccatatt
    32401 gcgctaattc atcatcaaac attggtagga tgcgagttgc tgcctctaca caaataattt
    32461 caatttgttc aggttttaca cccgctaaag cagcatattt tggtcgctca tcaaccatag
    32521 ctccggctaa ctcaatacct gtaaaaccag caccacatac taagagtttg agaagatttt
    32581 catcttttgt ctcacgatac aacttcattg tattaacaat gtgattatgg atgttttctg
    32641 cttgcgaaat attggtcatc tgcaaagcat tctccatagc acccttgatg ccaaaagttt
    32701 cagatacaaa gcctaaggca actacgacat aatcataatg gagttcaccg ttatttttaa
    32761 ctgttactgt tttattttca gcatcgactc taagcacctc atcttgcatg aagttaacct
    32821 tattagtgtt gataacatcc ctaattggga atgtaatttt ttcttttggc tgagatcctg
    32881 cagctacttc atgtaattct gtagcttcgt agtgataatc attcatgtca acaagggtaa
    32941 tatgaaaatc acctgattgt ttttgtaaat tacgaactgc tttcaaaccg gcataaccag
    33001 cacctaaaac taggatttct ttcatgataa aactcctcta aaaaatgatt ccaattagat
    33061 acgttaagac ttgggttgca gatcccagtg ctagaatacg taccgcacaa acaaatgttt
    33121 ctctcttaac ctgtttttgc cacaatagtt ttgtctgctt ataaataaat ggtattaata
    33181 acaaactcaa gagtactgta acaggcgcaa gtccaaaaag aaattcaaaa acaattgcca
    33241 ataaagctat actattagaa atagcaaaca gcaagagtcc acctcttatc cctgtataat
    33301 gtacaagggt atagcgatga tttttttcat cttcttcttt gtcgcacaga ttattcgcca
    33361 acattaggtt ggcaatccat aaggtgttcg gcaaggaaat caagaaaatt ttgcttaagt
    33421 tgaggatatc ccacgaaaag ttttgataag tgtttagata tacacaaatt aaactaatca
    33481 taaatcccat tgtcaagcca gaaaatactt ctcctaaggg cagactagat aaaggtcttg
    33541 gaccaaagga atataaaaca ccaattccaa aacaaaaaag tcccatccat aaaagaggca
    33601 gaccgacttg ggaaactaaa actaaaccaa tcatactaga agtaatgact aaacttgcca
    33661 taataacttc tatttggcgt agggataggt tctcacgacc aataatgtta gtgtcattct
    33721 tataatccaa atccacagca ttccgataat cattgtaatt attccaaatg tcaacgaaca
    33781 tattgaataa aaacatagca acgaagaata aaccgacata tacagggtgt actgaattgt
    33841 agtaataagc actaaaacac aaaccaatca aaaatggtaa aacactagct gttttggctt
    33901 tcatttctac caactctaga aagattggta atgtcattat taaagtcctc tctatacata
    33961 atcttcaaca atactattat aatgcaattt ctaccatttt taaaacaata gggcttgatt
    34021 tttgattaaa aatacaaaaa atttttctta aaatgagtta atcacactta gaacactaaa
    34081 tactattttg aggagtccta ttcatcacac tatgccatgt aatattcgga aaaatagtat
    34141 tgcaaccttc aattactcct tatgttggca ccagcctttg aaagtcctct gattgcactg
    34201 cttagttagt attttcatca gctgtatggt ctctaagagc catcagcata ctaaaaaaac
    34261 tagtgttaca ctttgaaaag tgtaacacta gctccaattt gttttaaata gcttaactat
    34321 ttaatcatta atctacttta tctatttaaa catttaaagt tgaatgctgg ttaaggatac
    34381 ctgagagaga accttgaata tattttggca acacttgttt ttgggcacgt tcatagattt
    34441 ctgaatctgt taactgacgt tctgctttgg ggcttgatac ttgcggtgtc actgctgcgt
    34501 tttgttgttg gtaaagtgtt gaatgttggt tgagaatacc tgaaagagaa ccttggatat
    34561 attttggtaa tacttgtttt tgggcacttt cataaagttc tgaatctgtt aactgacgtt
    34621 ctgctttggg gcttgatact tgcggtgtcg ctgctgcgtt ttgttgtttg taaagtgttg
    34681 aatgttggtt gagaatacct gaaagagaac cttggatata ttttggcaat acttgttttt
    34741 gggcacgttc ataaagttct gcatctgtta actgacgttc tgcttttgga ggtgttgctt
    34801 gcggtgctgt gaaagttgaa gctgaaacac cttgtgtaga accaagagct acgaatgctc
    34861 ctccagcaaa cattgctgag acaagaccga gtttcaaata gttagcagtt gagacgttgt
    34921 tgactttttt tctgtaataa gttttttcat aagaattacc tctttctttt gggttattta
    34981 ttttatgaac taagtataac aacaagaaat aaagtaag
//
LOCUS       DATRNK010000015        34594 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.015, whole genome shotgun sequence.
ACCESSION   DATRNK010000015 DATRNK010000000
VERSION     DATRNK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34594
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.015"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            32..583
                     /locus_tag="VBC52_000729"
     CDS             32..583
                     /locus_tag="VBC52_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEO6882330.1"
                     /translation="MTKIYHMILCNSDIMKILAIIKSLPLNDCWLCAGTLRNFVWNKL
                     SGINETLTSDIDVVFFDKNISYEETVVLEQQLKDNYPQYDWELKNEFYMNTHSPNTPK
                     YTSSKDAISKFPEKCTAVGARLDDRNQLELYLPYGEEEILNFIVSPTPYFEEDLLRYN
                     VYLKRVDKKKWNNIWPRLTIIKK"
     gene            803..1222
                     /locus_tag="VBC52_000730"
     CDS             803..1222
                     /locus_tag="VBC52_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HEO6882331.1"
                     /translation="MKMTVYFDGNFWLGLIEYDDDGDYKVFRYFFGKEPKDDDVFNFI
                     NHKLNDLIKKYEFVKTDISLKRTNEHKKSPKRMQREINREKRKPVVSTKAQLAMKTIH
                     MSIKNERQLSQKCKKNELRKHRYQLKQEKRYQKKKGH"
     gene            1422..1901
                     /locus_tag="VBC52_000731"
     CDS             1422..1901
                     /locus_tag="VBC52_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6882332.1"
                     /translation="MRDFEELFDKVKPIVMKLRRNYFVQLWEYDDWIQEGRIVLFRLL
                     EEHPYLLDNESKLFIYFKTKFSNYLNDVLRHQDCQKRQFNKMPYEEISEVSHYVKSKG
                     LVLDDYIAYRDTLTKVEETLSDIDKEKFEKLISGERFAGKKQFIRDIQPFFNAFKAD"
     gene            2023..2715
                     /locus_tag="VBC52_000732"
     CDS             2023..2715
                     /locus_tag="VBC52_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO6882333.1"
                     /translation="MSKVRLYIARHGKTMFNTIGRAQGWSDTPLTTFGELGIKELGLG
                     LKASNISFKEAFSSDSGRTLQTMEIILREVQQENIPYTRDKRIREWCFGSLDGGYDGD
                     LFNGVLPRVSNGDMSHLTHEEIANLICQVDTAGWAEPWAILSNRILSGFTAIAKKIED
                     IGGGNAIVVSHGMTIATFLWLIDHSTPRSLGLDNGSVSVVDFEDGTFSIQSIGDMSYR
                     EKGREILEKTLQ"
     gene            2712..3464
                     /locus_tag="VBC52_000733"
     CDS             2712..3464
                     /locus_tag="VBC52_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEO6882334.1"
                     /translation="MKKNKIIRFSLVGVLLAILCFSLFALLKPNSQQSSSQKLRNEDI
                     KKISSQKRNKKLQLPAVSSKDWNLILVNRDHKHEELSPDVVPVENIYLDKRITKQATQ
                     FLEAARAIDSREHLISGYRSVAYQEKLFNSYVTQEMTSNPNLTRGQAEKLVKTYSQPA
                     GASEHQTGLAMDMSTVDSLNESDPRVVSQLKKIAPQYGFVLRFPDGKTAETGVGYEDW
                     HYRYVGVESAKYMAKHHLTLEEYITLLKENNQ"
     gene            3461..4036
                     /locus_tag="VBC52_000734"
     CDS             3461..4036
                     /locus_tag="VBC52_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEO6882335.1"
                     /translation="MRKRFSLLNFIVVTFIFFFFILFPLLNHKGKVDANSRQSVTYTK
                     EEFIQKIVPDAQDLGKSYGIRPSFIIAQAALDSDFGEKILANKYHNLFGLLAEPGTPS
                     ITLNDSSTGKKQEKQFTHYKSWKYSMYDYLAHIKSGATGKKDSYTIMVSVKNPKTLVQ
                     KLQDSGFDNDKKYAKKMTEIIDLYDLTRYDK"
     gene            4131..5213
                     /gene="hrcA"
                     /locus_tag="VBC52_000735"
     CDS             4131..5213
                     /gene="hrcA"
                     /locus_tag="VBC52_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEO6882336.1"
                     /translation="MCKSANFRKTERGDVFVITQRQNDILNLIVELFTQTHEPVGSKA
                     LQRTIDSSSATIRNDMAKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQD
                     IYHVIKAFDFEAFKLEDMLQKASHILSEMTGYTSVILDVEPARQRLTGFDVVQLSNHD
                     ALAVMTLDESKPVTVQFAIPRNFLTRDLIAFKAIVEERLLDGSVIDIHYKLRTEIPQI
                     VQKYFVTTDNVLQLFDYVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLR
                     QSLKEGEMASVQVADSQEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLV
                     NIIGKVLAAKLGDYYRYLNSNHYEVH"
     gene            5216..5788
                     /gene="grpE"
                     /locus_tag="VBC52_000736"
     CDS             5216..5788
                     /gene="grpE"
                     /locus_tag="VBC52_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEO6882337.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEEIP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            5969..7798
                     /gene="dnaK"
                     /locus_tag="VBC52_000737"
     CDS             5969..7798
                     /gene="dnaK"
                     /locus_tag="VBC52_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEO6882338.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVEEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIQSNSGLTDEEIDKMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKKAQESGNLDDMKAKLEALNEKAQ
                     ALAVKLYEQAAAAQQAAQGAEGAQSADSSSKGDDVVDGEFTEK"
     gene            8087..9226
                     /gene="dnaJ"
                     /locus_tag="VBC52_000738"
     CDS             8087..9226
                     /gene="dnaJ"
                     /locus_tag="VBC52_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEO6882339.1"
                     /translation="MNNTEFYDRLGVSKDASQDEIKKAYRRMSKKYHPDINKETGAEE
                     KYKEVQEAYETLSDTQKRAAYDQYGAAGANGGFGGFDGGGFGGFDGGGFGGFEDIFSS
                     FFGGGGMRNPNAPRQGDDLQYRVNLSFEEAIFGAEKEVSYNRESSCHTCSGSGAKPGT
                     SPVTCQKCHGSGVINVDTQTPLGTMRRQVTCDVCQGSGQEIKEKCPTCHGTGHEKKTH
                     KVSVKIPAGVETGQQIRLTGQGEAGFNGGPYGDLFVIINVLPSQQFERNGSTIYYTLN
                     ISFVQAALGDTIDIPTVHGAVEMSIPAGTQTGKTFRLRGKGAPKLRGGGQGDQHVTVN
                     IVTPTKLNDAQKEALHAFAEASGDKMVHPKKKGFFDKVKDALDVD"
     gene            9340..10587
                     /locus_tag="VBC52_000739"
     CDS             9340..10587
                     /locus_tag="VBC52_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEO6882340.1"
                     /translation="MVTKVEEIRSYLIASIQNGKLAPGDRLPSIRQLANQFSCNKDTV
                     QRVLMELRFDNYIYAKPRSGYYVFDSHQEEVEEGVSLPNSEIANIAYDDFRLCLNETL
                     IGREDYLFNYYYRQEGLLDLSKAVAKLMEETGVYVPLDDIVITAGTQQALFILTQVTF
                     PNRKSRVLIEEPTYPRMIELIKTQNLPYETISRGTHGIDFQRLEEIFQTQSIKFFYVI
                     PRMHNPLGTSYNPVEMKRLIEMAEKYDVYIVEDDYMSDFASQSPLHYYDTHGRVIYLK
                     SFSKAIFPALRLAAICLPQALKSTFMAYKKLMDYDTNLILQKALALYIENGLYAKNSQ
                     YLKYRYQKDLANSKSILADHPNLPSYSLHHDSVLFDCSKLDNFKILRQYGDTLENYFC
                     QKSHQSLLQVKNDSCLKQFLGSL"
     gene            10658..11434
                     /gene="truA"
                     /locus_tag="VBC52_000740"
     CDS             10658..11434
                     /gene="truA"
                     /locus_tag="VBC52_000740"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEO6882341.1"
                     /translation="MTRYKAQISYDGSAFSGFQRQPNCRTVQEEIERTLKRLNSGNDV
                     IIHGAGRTDVGVHAYGQVIHFDLPQARDVEKLRFGLDTQCPDDIDIVKVEQVSDDFHC
                     RYDKHIKTYEFLVDIGRPKNPMMRNYATHYPYPVIIELMQEAIKDLVGTHDFTGFTAS
                     GTSVENKVRTIFDAKIQFEASKNLLIFTFTGNGFLYKQVRNMVGTLLKIGNGRMPISQ
                     IKTILQAKNRDLAGPTAAGNGLYLKEIIYEDEECFSNFRK"
     gene            11397..12155
                     /locus_tag="VBC52_000741"
     CDS             11397..12155
                     /locus_tag="VBC52_000741"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO6882342.1"
                     /translation="MKTRNVLAISGNDIFSGGGLHADLATYVVNKLHGFVAVTCLTAM
                     SDKGFEVIPIEASILKQQLESLKDVEFGSIKLGLLPNVETAQVVLEFVKSKQECPVVL
                     DTVLVCKENHDLEVSQLREQLIAFFPYADVITPNLVEAQLLTGLSIENLDQMKIAAEK
                     LYDMGAKHVVIKGGNRLNAEEATDLYYDGERFETYVFPVVDANNTGAGCTFASSIASQ
                     LAMGKNVEDAVKMSKGFVYQAIKASDKYGVVQHF"
     gene            12165..12629
                     /locus_tag="VBC52_000742"
     CDS             12165..12629
                     /locus_tag="VBC52_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6882343.1"
                     /translation="MRKEKTSQLVQLAIVTAISIVLGMFISIPTPTGFLTLLDAGIFF
                     AAFYFGKKEGAVVGALAGFLIDLLKGYPNWMFFSLLIHGTQGYLAGLPGRRRLLGLIS
                     ATLVMVLGYAIASGLMYGWGAVLPDIPGNIMQNMVGMVVGFALNKSLERVKK"
     gene            12626..13195
                     /locus_tag="VBC52_000743"
     CDS             12626..13195
                     /locus_tag="VBC52_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEO6882344.1"
                     /translation="MILDYNKLKQETKAIVVDIIERSALKKGQIFVLGLSSSEVSGGL
                     IGKNSSSEIGEIIVEVILKELHSRGIYLAVQGCEHVNRALVVEAELAERQQLEVVNVV
                     PNLHAGGSGQVAAFKLMTSPVEVEEIVAHAGIDIGDTSIGMHIKRVQVPLIPISRELG
                     GAHVTALASRPKLIGGARAGYTSDPIRKF"
     gene            complement(13232..14074)
                     /locus_tag="VBC52_000744"
     CDS             complement(13232..14074)
                     /locus_tag="VBC52_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEO6882345.1"
                     /translation="MITLEKFIDHLNVEEVLFTFFTKLISILLLIIAFVIVRQVINYL
                     FEKTVNRSLAFSRQKVARQKTLAKLSHNVLNYTLYFFLFYWILSILGVPISSLLAGAG
                     IAGVAIGLGAQGFLSDVVNGFFILLENQFDVGDIINVGTVSGTVTNVGIRTTQIHDFD
                     GTLHFIPNRNITIVSNKSRSNMRAQIDIPLFVHTNLDQISDIVTKINEEYVSKHPAIV
                     GEPTVFGPTTNANGQFVYRINIFTQNGAQFDIYAEFYKLYQKAILEEGIDLPTYNFSN
                     NHSR"
     gene            14231..15514
                     /locus_tag="VBC52_000745"
     CDS             14231..15514
                     /locus_tag="VBC52_000745"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEO6882346.1"
                     /translation="MSTSFENKATNRGIITFTISQDEIKPALDQAFNKVKKDLNVPGF
                     RKGHMPRTVFNQKFGEEALYENALNLVLPKAYEAAVAELGLDVVAQPKIDVVSMEKGQ
                     DWKLTAEVVTKPEVKLGDYKDLSVEVDASKEVSDEEVDAKVERERNNLAELTVKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEEQLVGSKAGQTVDVNVT
                     FPEDYQAEDLAGKDAKFVTTIHEVKTKEVPALDDELAKDIDDEVETLDELKAKYRKEL
                     ESAKEIAFDDAVEGAAIELAVANAEIVELPEEMVHDEVHRAMNEFMGNMQRQGISPEM
                     YFQLTGTTEEDLHKQYQADADKRVKTNLVIEAIAAAEGFEATDEEIEKEITDLASEYN
                     MEADQVRGLLSADMLKHDIAMKKAVDVITSSATVK"
     gene            15703..16278
                     /locus_tag="VBC52_000746"
     CDS             15703..16278
                     /locus_tag="VBC52_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEO6882347.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILEQRGRDNEMYFSDLVNDIQT
                     YLGKSDSAIRESLPFFYSDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     APKRKKKRVNAFMDGDEDAIDYNDDDPEDEDFTEETPSLEYDEENPDDEKSEVESYDS
                     EINEIIPDEDLDEDVEINEEDDEEEEEEEEV"
     gene            16551..18155
                     /locus_tag="VBC52_000747"
     CDS             16551..18155
                     /locus_tag="VBC52_000747"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEO6882348.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLKKE
                     RRGEYLGATVQVIPHVTDALKEKIKRAATTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSDNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEQPAG
                     QSIKNKLAQFCDVAPEAVIESLDVDHIYQIPLNMQAQNMDQIVCDHLKLETPAADMTE
                     WSAMVDKVMNLEKKVKIALVGKYVELPDAYLSVVEALKHSGYVNDVAIDLKWVNAAEV
                     TEDNIKELVGDADGIIVPGGFGQRGSEGKIEAIRYARENDVPMLGVCLGMQLTCVEFA
                     RNVLNLHGANSAELDPKTPFPIIDIMRDQIDIEDMGGTLRLGLYPCKLKSGSRAAAAY
                     NNQEVVQRRHRHRYEFNTKFREQFEAAGFVFSGVSPDNRLMEVVELPEKKFFVAAQYH
                     PELQSRPNHAEELYTAFVTAAVENMK"
     gene            18264..19190
                     /locus_tag="VBC52_000748"
     CDS             18264..19190
                     /locus_tag="VBC52_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6882349.1"
                     /translation="MKKIRLSKFIKMIVVILFLISVAASFYFFHVAQVRDDKSFISNG
                     QRKPGNSLYAYDKSFDKLLKQKIEMTNQNIKQVAWYVPAVKKTHKTAVVVHGFANSKE
                     NMKAYGWLFHKLGYNVLMPDNIAHGESHGQLIGYGWNDRENIIKWTEMIVDKNPSSQI
                     TLFGVSMGGATVMMASGEKLPSQVVNIIEDCGYSSVWDELKFQAKEMYGLPAFPLLYE
                     VSTISKIRAGFSYGQASSVEQLKKNNLPALFIHGDKDNFVPTSMVYDNYKATAGKKEL
                     YIVKGAKHAKSFETEPEKYEKRISSFLKKYEK"
     gene            19237..19322
                     /locus_tag="VBC52_000749"
     tRNA            19237..19322
                     /locus_tag="VBC52_000749"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19271..19273,aa:Leu,seq:aag)
     gene            19385..19831
                     /locus_tag="VBC52_000750"
     CDS             19385..19831
                     /locus_tag="VBC52_000750"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEO6882350.1"
                     /translation="MSKVRGFELVSQFSNKELLPKRETAHAAGYDLKVAKKTVIEPGE
                     ITLVPTGIKAYMQPGEVLYLYDRSSNPRKKGIVLINSVGVIDGDYYNNQVNEGHIFAQ
                     MQNITDQAVILEEGERIVQAVFAPFLLADDDQATGMRTGGFGSTGK"
     gene            19993..21357
                     /gene="radA"
                     /locus_tag="VBC52_000751"
     CDS             19993..21357
                     /gene="radA"
                     /locus_tag="VBC52_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEO6882351.1"
                     /translation="MAKKKSVFTCQECGYQSPKYLGRCPNCSAWSSFVEEVEVQEVKN
                     ARVSLNGEKSRPTKLKDVSSINYSRTKTDMDEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQSIRSEIE
                     KIKPDFLIIDSIQTIMSPEVSSVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTVFGNAKRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQESFIGE
                     IGLTGEIRRVTRIEQRINEASKLGFTKIYAPKNSLAGIEIPKGIDVIGVTTVSQVLKA
                     VFST"
     gene            21493..21990
                     /locus_tag="VBC52_000752"
     CDS             21493..21990
                     /locus_tag="VBC52_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEO6882352.1"
                     /translation="MTTYFDNFLKTNQAYADLHGTAHLPIKPKTKVAIVTCMDSRLHV
                     AQALGLALGDAHILRNAGGRVTDDVLRSLVISQQQLGTREIVVLHHTDCGAQTFTNEA
                     FAAQLQRDLGVDMHGHDFLPFNDIEESVREDVAKLHASPLIPDDVVISGAIYDVDTGR
                     MVEVK"
     gene            22144..23463
                     /locus_tag="VBC52_000753"
     CDS             22144..23463
                     /locus_tag="VBC52_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEO6882353.1"
                     /translation="MKKYDVIVLGFGKAGKTLAAKLATQGKSVAMVEEDDKMYGGTCI
                     NIGCIPTKTLLVSASKNHDFQEAMTTRNEVTSRLRAKNFAMLDNKDTVDVYNAKARFI
                     SNKVVELTGGADKQELTADVIIINTGAKSVQLPIPGLADSQHVYDSTAIQELAHLPKR
                     LGIIGGGNIGLEFATLYSELGSKVTVIDSQSRIFAREEEELSEMAQDYLEEMGISFKL
                     SADIKSVQNEDEDVVISFEDEKLSFDAVLYATGRKPNTEGLALENTDIKLTERGAIAV
                     DEYCQTSVENIFAVGDVNGGPQFTYISLDDSRIVLNYLNGDKDYSLKNRGAVPTSTFT
                     NPPLATVGLDEKTAKEKGYQVKSNSLLVSAMPRAHVNNDLRGIFKVVVDTETNLILGA
                     RLFGAESHELINIITMAMDNKIPYTYFQKQIFTHPTMVENFNDLFNF"
     gene            23672..25126
                     /locus_tag="VBC52_000754"
     CDS             23672..25126
                     /locus_tag="VBC52_000754"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEO6882354.1"
                     /translation="MANKIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGDFVIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPETHENYRQSERLELYQRYIDQLLAEGKA
                     YKSYVTEEELAAERERQELAGETPRYINEFIGMSETEKEAYIAEREAAGIIPTVRLAV
                     NESGIYKWTDMVKGDIEFEGSNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPQFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     SEAVFNFIALLGWNPGGEEEIFSREQLINLFDENRLSKSPAAFDQKKMDWMSNDYLKN
                     ADFESVFALCKPFLEEAGRLTDKAEKLVELYQPQLKSADEIVPLTDLFFADFPELTEA
                     EKEVMAAETVPTVLSAFKEKLVSLSDEEFTRDTIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIYLLGKEKSVQHIDNMLAKL"
     gene            complement(25166..26134)
                     /locus_tag="VBC52_000755"
     CDS             complement(25166..26134)
                     /locus_tag="VBC52_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEO6882355.1"
                     /translation="MKFGKKLGFLALLMSIVLILGACGKTGLGNSSGNSTKNVTKKSA
                     KDLKLGVSISTTNNPYFVAMKDGIDKYASNKKISIKVADAQDDAARQADDVQNFISQN
                     VDAILINPVDSKAIVTAIKSANNANIPVILMDRGSEGGKVLTTVASDNVAAGKMAADY
                     AVKKLGKKAKAFELSGVPGASATVDRGKGFHSVAKSKLDILSSQSANFDRAKALNTTQ
                     NMIQGHKDVQIIFAQNDEMALGAAQAVKSAGLQNVLIVGIDGQPDAHDAIKKGDISAT
                     IAQQPAKMGEIAIQAAIDYYKGKKVEKETISPIYLVTKDNVEKYNW"
     gene            complement(26187..27128)
                     /gene="rbsC"
                     /locus_tag="VBC52_000756"
     CDS             complement(26187..27128)
                     /gene="rbsC"
                     /locus_tag="VBC52_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771495.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HEO6882356.1"
                     /translation="MKNMMKYISELTTLIALAGLMVIITLINPNFLTTNNLLNLLLQV
                     TANGFIAFGMTFVILTGGIDLSVGSILALSSALTAGLIGHGVPVGIAIVLSVALGGIL
                     GMLNGLFISYGKLAPFIVTLATMTIFRGATLVYSNGNPITAGLSDSFLFQFLGQGYIV
                     GIPFPVILMFLTFIILYILLHKTAFGKSVYALGGNEKAAYISGIKLNKVKIIIYTISG
                     IMASISGLIITSRLSSAQPTAGASYEMDAIAAVVLGGTSLSGGKGRIIGTLIGALIIG
                     VLNNGLNIIGVSAFWQQVVKGIVILMAVLLDRFKVAK"
     gene            complement(27130..28608)
                     /locus_tag="VBC52_000757"
     CDS             complement(27130..28608)
                     /locus_tag="VBC52_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEO6882357.1"
                     /translation="MKIDMRNISKSFGTNKVLEKIDLELQSGQIHALMGENGAGKSTL
                     MNILTGLFPASTGTIYIDGEERTFSNPQEAEEFGISFIHQEMNTWPEMTVLENLFLGR
                     EIKTTFGLLNQKLMRQKALETFKRLGVTIPLDIPIGNLSVGQQQMIEIAKSLLNQLSI
                     LVMDEPTAALTDRETENLFRVIRGLKQEGVGVVYISHRMEEIFKITDFVTVMRDGVIV
                     DTKETSLTNSDELVKKMVGRKLEDYYPEKHSEIGPVAFEVSNLCGDNFEDVSFYVRKG
                     EILGFSGLMGAGRTEVMRTIFGIDKKKSGKVKIDDQEITITTPSQAIKQGIGFLTENR
                     KDEGLILDFNIKDNMTLPSTKDFSKHGFFDEKTSTTFVQQLINRLYIKSGRPDLEVGN
                     LSGGNQQKVVLAKWIGIAPKVLILDEPTRGVDVGAKREIYQLMNELADRGVPIVMVSS
                     DLPEILGVSDRIMVMHEGRISGELSRKEADQEKVMQLATGGK"
     gene            complement(28624..29022)
                     /gene="rbsD"
                     /locus_tag="VBC52_000758"
     CDS             complement(28624..29022)
                     /gene="rbsD"
                     /locus_tag="VBC52_000758"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEO6882358.1"
                     /translation="MKKTGILNSHLAKLADDLGHTDRVCIGDLGLPVPNGIPKIDLSL
                     TSGIPSFQEVLDIYLENILVEKVILAEEIKEANPDQLSRLLAKLDNSVSIEYVSHNHL
                     KQMTQDVKAVIRTGENTPYSNIILQSGVII"
     gene            complement(28997..29908)
                     /gene="rbsK"
                     /locus_tag="VBC52_000759"
     CDS             complement(28997..29908)
                     /gene="rbsK"
                     /locus_tag="VBC52_000759"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEO6882359.1"
                     /translation="MSNIVIIGSISMDLVMETNRIAKEGETVFGQRFSMVPGGKGANQ
                     AVAIGRLSQERDNITILGAIGEDSFGPILLDNLNKNHVTTDFVGTIPSSSGVAQITLY
                     NNDNRIIYCPGANGKVDTKKWSQEWSIIKEADLVVLQNEIPHQANMKIANFCKEHSIK
                     LLYNPAPSRETDIEMLDKVDYFTPNEHECQELFPNQKLEDILATYPEKLIVTLGTKGA
                     IYSDGKESHLIPALETKAVDTTGAGDTFNGAFGYAISKKFKIAKALRFATLAAHLSVQ
                     KFGAQGGMPTIKEMEDNQHYEKDWYIK"
     gene            complement(29901..30887)
                     /locus_tag="VBC52_000760"
     CDS             complement(29901..30887)
                     /locus_tag="VBC52_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6882360.1"
                     /translation="MSTIRQVAEKAGVSTSTVSRYISQNGYVSQKASQKIEQAIRELH
                     YVPNFLAQSLKTKKNQLVGLLLPDISNPFFPRLARGVEEFLKEQGYRVMLGNTNNKSH
                     LEEEYLNVLLQSNAAGIITTHDFTKNHPEIDIPVVVVDRVNQETQYSVFSDNKEGGKL
                     AAQAIWTAGATNILLIRGPLDKADNLNQRFQGSQNYLLNKGACFAIEDSASFDFAEIQ
                     IEAKTLLDHHPDIDSIIAPSDIHAIAYLHEILNRGKRIPEDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYIMGQKAAELIFKITNQLPITNKRIKLPVHYVERETLRRKIDE"
     gene            31069..32157
                     /locus_tag="VBC52_000761"
     CDS             31069..32157
                     /locus_tag="VBC52_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEO6882361.1"
                     /translation="MSHAWKELTFYKKKYLLIELLIIVMMFMVVFLSGLANGLGRAVS
                     AAIENNPAQTYILNEGAEQVITSSVLTTKDQTDLNSLNLKDSTTLNIQRSSLTRQGHE
                     KKIDISYFAIDKDSFMAPTLSEGKQLTSYKKAIILNDSLKAEGIKLGDKVIDKSSSIS
                     LTVVGFVHNSMYGHGPVAFIDKDIYTEINKKINPQYQFLPQALVMKNDKSISHLPTQL
                     EAVSKKDVIQHIPGYSAEQSTLNMILWVLVVASAGILGVFFYIITLQKRHEFSVMKAI
                     GTKMSEIALFQLSQVIILALFGIIVGDGLAVALSYVLPAQMPFVINWQNIILVSFVFL
                     VIAMISSALSIVKVAKIDPVEVINGGGE"
     gene            32157..32843
                     /locus_tag="VBC52_000762"
     CDS             32157..32843
                     /locus_tag="VBC52_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6882362.1"
                     /translation="MSAILELKHISKHYPDGDELLSILDNLDLSVSAGEFVAILGPSG
                     SGKSTLLSIAGLLLGADQGSLYVNHENVTDLSQRQRTQLRREALGFIFQSHQLLPYLT
                     IQEQLQQEARFAKHYDKKTSLEEINKLLSDLGIEQCAHKYPNQLSGGQKQRAAIARAF
                     INHPKVILADEPTASLDEERGRQVTELIRQEVKSHNTAAIMVTHDERVLDLVDTVYRL
                     KDGKLVKENF"
     gene            32874..33545
                     /locus_tag="VBC52_000763"
     CDS             32874..33545
                     /locus_tag="VBC52_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6882363.1"
                     /translation="MIKILVVEDNIVQQKIITTKLTQEGYQFITASNGQEALNCLDTE
                     EVQLIITDIMMPMMDGYQLIQELRSAAYNVPIIVMTAKSQMEDMTKGFGLGADDYMVK
                     PVQLQELALRIKALLRRANIVAQHQLIIGNTCLNEDELSLKYFEQEIIFPQKEFRVLF
                     HLLSYPNRIFTRLELLDSIWGMDTDLDERVVDACINKIRRKVEHLPDFKIETVRGVGY
                     RAKND"
     gene            33529..>34594
                     /locus_tag="VBC52_000764"
     CDS             33529..>34594
                     /locus_tag="VBC52_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6882364.1"
                     /translation="MQKMTKLRRFRFPLRFYFTLMFVLTMLFSVLASLLLVAAIVFTF
                     FQGVLTTHVLQVSALAVVFLSLVIASISMWYGSYHLTKPILDISHIVSNVADGDFEGH
                     IYRNSNRRKSYEYYNELDELSESINQMIVSLSHMDHMRKDFITNVSHELKTPIAAVAN
                     IVELLQDPELDEETQSELLGLVKTESLRLTRLCDTMLQMSRVDNQETIGELSSVRVDE
                     QIRQAMISLTERWQAKRINFQLDSKPYTVYSNSDLLMQVWINLLDNAIKYSEDIVDLS
                     VRMEETNNHYLRVIISDKGRGISQYDVQHIFDKFYQADQSHNQQGNGLGLAIVKRIIV
                     LCKGRISVSSQLEIGTEFCVE"
BASE COUNT        11286 a         5513 c         7003 g        10792 t
ORIGIN      
        1 cattgtctgg agatgtagca aaggagataa aatgactaaa atttatcata tgattttatg
       61 taattctgat ataatgaaga ttttagctat aataaaatct cttccactga atgattgttg
      121 gctgtgtgct ggcacattga gaaactttgt atggaataaa ctttctggaa taaatgagac
      181 attgacatca gatattgatg ttgtattttt tgataaaaat atttcttatg aagaaacggt
      241 ggtattagaa caacaattaa aggataacta tccacagtac gattgggaac ttaaaaatga
      301 attttacatg aacactcatt ctcctaatac cccaaagtat actagctcta aagatgctat
      361 ttcaaaattt cctgaaaagt gtactgcagt gggagcaagg ttagatgata gaaatcaact
      421 cgaactttac ctcccttatg gtgaagagga aatattaaat ttcattgtta gtcctacgcc
      481 atattttgaa gaggatctat tacgttataa tgtttatttg aagcgagtgg ataagaagaa
      541 atggaataat atttggcctc gtttaacaat cataaaaaaa tgataacgtt actggagttg
      601 gcttaacagc ttttaaattt ttagactgac aaatagtata gacctttaat aaggtcgttt
      661 atcgataatc atagtgtact gtgttataat cgttatagca taaaaacacg ttgcagttgg
      721 tagtctgcaa gcgttcagtg aacgtcagta accttccctc caggtcgtcc attgcaattt
      781 aatatttaaa ggagggggct gtatgaaaat gacagtctat tttgatggta atttttggtt
      841 gggtttgatt gagtacgatg atgatggtga ttacaaagtt tttcggtatt tttttggaaa
      901 agaacctaaa gatgatgatg tttttaattt tataaatcat aaacttaatg atttgataaa
      961 aaaatatgag tttgttaaaa cggatatttc tctaaagcgt acgaatgaac acaaaaaaag
     1021 tcctaaaaga atgcaaagag aaattaatag agaaaaacgt aagccagtag tatctacaaa
     1081 agcgcaattg gcgatgaaaa ctattcatat gagtataaaa aacgaacgtc aattaagtca
     1141 gaaatgtaag aaaaatgagt taagaaaaca tcggtaccaa ttaaaacaag aaaaacgtta
     1201 ccaaaagaaa aaaggacatt gattgtgtca tcatataaaa agcttgagag tgtcatctca
     1261 agctttttat atgttaacta atatgagtca ctaaatagtc tgataatgct caattaatgt
     1321 gttttgggaa aaaatcattt ccttaaagga caccattgtc atgttgttat caccttactt
     1381 tttttatact tagtatatgt ttaagaaagg aataagttgt tttgagagat tttgaggagt
     1441 tgtttgataa agtaaagcca atcgtaatga agctgagaag aaattatttt gttcaacttt
     1501 gggaatatga tgattggatt caagaaggac gtattgtact ttttaggttg cttgaagagc
     1561 atccgtattt attggataat gaaagcaaac tattcattta ctttaaaaca aaattttcaa
     1621 attatttaaa cgatgtatta agacaccaag attgtcaaaa gcgacaattt aataaaatgc
     1681 cttatgaaga aataagtgaa gttagtcact atgttaagag taaaggacta gttttagatg
     1741 actatattgc ttatcgtgat actttaacaa aagtggaaga aactttgtca gatattgata
     1801 aagaaaaatt tgaaaaactg attagtggtg aacgttttgc ggggaagaaa caattcatac
     1861 gagatattca accttttttc aatgctttta aagcggatta atcgatttta tttctgcagt
     1921 agatagctgc agaatttttt tatagggaaa gttcgctgat atattcctgt gaaagccttt
     1981 gttttttgtt ataatataat aaaatgcttg tgaggtgtca gtatgagtaa agttcgttta
     2041 tatatcgctc gtcatggtaa aacaatgttt aatacaattg gtcgcgctca ggggtggagt
     2101 gatactcctt taactacttt tggagaactc gggattaaag agcttggttt gggactaaag
     2161 gcttcaaata ttagttttaa agaggctttt tctagtgata gtggacgtac tttacaaact
     2221 atggaaatta ttctcaggga agtgcaacaa gaaaatattc cctatacgag agataaacga
     2281 atccgcgagt ggtgctttgg tagtttggat ggaggatatg atggcgacct ttttaatggc
     2341 gttctgcctc gagtttcaaa cggagatatg agccatttga cacacgaaga aattgctaat
     2401 ttgatttgtc aagttgatac ggctggctgg gcagaaccgt gggcaatatt gagtaaccgt
     2461 attctgtcag gcttcactgc tatagctaaa aaaatagagg atattggggg aggtaatgcg
     2521 attgttgtca gtcatgggat gactattgcg acttttttat ggttaatcga ccatagtacc
     2581 cctcgtagtt taggcttaga taatggtagt gtctcagtcg ttgattttga agatggaaca
     2641 ttttctattc aatctattgg ggatatgtcc tatcgagaaa aaggaagaga aattttagaa
     2701 aaaacattgc aatgaaaaaa aataaaatta tccgattcag tttagttggt gttctacttg
     2761 cgatactatg ctttagtctt tttgctttat tgaagcctaa cagtcaacaa tcatcatctc
     2821 aaaagttgag gaatgaggat ataaaaaaga tatcctctca aaaaagaaat aagaaattac
     2881 aattaccagc tgtatcatca aaagattgga acttgatttt ggtcaatcgt gaccataaac
     2941 atgaagaatt aagtccagat gtggttcctg ttgaaaatat ttatttggat aaacgtatta
     3001 cgaagcaagc tactcagttt ttagaggctg ctagagcaat tgattcacga gaacatttaa
     3061 tttcgggtta tcgtagtgtt gcctatcagg agaagttgtt caattcttat gttactcaag
     3121 agatgactag taaccctaat ttgacgaggg gacaagcaga aaagttggta aaaacttact
     3181 ctcagcctgc aggtgctagt gaacaccaga ctggattagc gatggatatg agtactgtag
     3241 attctttgaa tgagagcgat cctagagtag tcagtcagtt gaaaaagata gctccacaat
     3301 atggttttgt cttacggttt ccggatggta aaacagcaga aacaggggta ggttatgaag
     3361 attggcatta ccgctatgtt ggggtagagt ctgcaaaata tatggccaaa catcatttaa
     3421 cattagaaga atacataact ttattaaagg agaataacca atgaggaaac gtttttcctt
     3481 gctaaatttt attgttgtta cttttatttt ctttttcttt attctttttc cgcttttaaa
     3541 ccataaggga aaagtagatg ctaattctag gcagagtgtt acctacacca aagaagaatt
     3601 tatacaaaaa attgtgccag atgcgcaaga tctaggaaag tcgtacggta ttcgtccttc
     3661 atttattatt gcacaggcgg ctttggattc tgatttcgga gagaaaatat tagctaataa
     3721 gtatcataat ctgtttggtt tgcttgcaga accaggaacg ccctcaatta ccttaaatga
     3781 tagtagtaca ggaaaaaaac aggaaaagca atttactcat tataaatctt ggaagtattc
     3841 aatgtatgat taccttgctc atataaaatc tggagcgaca ggcaaaaaag attcatatac
     3901 tataatggtg tctgttaaaa atccaaaaac tttagtgcaa aaattacaag atagtggttt
     3961 tgataatgac aaaaagtacg ctaaaaaaat gacggaaatc attgatttgt atgatttaac
     4021 aagatatgat aagtgatatt agcactcttt gaataagagt gctaattttt gatgtttttt
     4081 ccttgactat cttttaaaaa ggagtataat aaaatcatag ttagcagtcg atgtgtaaga
     4141 gtgctaattt cagaaagacc gagaggggtg atgtttttgt gattacacaa cgtcaaaatg
     4201 atattttgaa tttaatcgtt gagttattta cacaaactca tgaaccagta gggtctaaag
     4261 ctttacagag aacgattgat tctagtagcg ccactattcg taatgatatg gcaaaactag
     4321 agaagttggg gttattggaa aaagcacaca cttctagtgg acggatgccg agtccagctg
     4381 gttttaagta ttttgtagag cactccctta gactagatag tattgatgag caagatattt
     4441 atcatgttat aaaagctttt gactttgaag cattcaaatt agaagacatg cttcaaaaag
     4501 ctagtcatat tttatcggag atgacgggct atacatccgt tattttagat gtggagccag
     4561 ctcgtcaacg tttgacaggg tttgatgttg ttcagcttag caaccatgat gctctagcag
     4621 tcatgacttt agatgaatct aagccagtga cagtgcagtt tgctattcca agaaactttt
     4681 tgacacgtga cttgatagca tttaaagcta tcgtagagga gcgcttgctg gatggtagtg
     4741 tcatagatat tcattataag ttgaggacag aaattcctca gatcgttcag aagtattttg
     4801 taactacgga taatgtttta caactatttg attatgtttt ttcagagctg tttctggaaa
     4861 cggtatttgt agctggcaag gtaaattctt taacgtattc agatctatca acgtatcagt
     4921 ttttggataa tgagcagcaa gtagcgattt ctttgaggca aagtctgaaa gagggtgaga
     4981 tggcttctgt ccaagtggca gatagtcaag aagccgcact tgcagatgtg tcagttttaa
     5041 ctcacaaatt tttgattcct taccgtggat ttggactcct gagtttgatt ggtcctattg
     5101 atatggatta tcgtaggtct gttagtttgg taaacattat cggaaaggta ttggctgcta
     5161 aattaggtga ttattatcga tacctaaatt ctaaccatta tgaagttcac tgataatggc
     5221 agtattcaat aagttattta aaaggaggca ctcagtgtca gaagaaatta aaaaagacga
     5281 tttacaggaa gaagtggaag ccactgaaac tgaagagaca gttgaggaag ttattgaaga
     5341 aattcctgaa aaatcagagt tagaactcgc aaacgagcgt gctgatgagt ttgaaaataa
     5401 atacttgcga gctcatgcag aaatgcagaa tatacaacgt cgctctagtg aagaacgtca
     5461 acaacttcaa cgctatcgtt ctcaggatct ggcaaaagca atcttaccat cgcttgacaa
     5521 tctagaacgt gccttggctg ttgaagggtt gacagatgat gttaaaaaag gtttagaaat
     5581 gactcgtgat agccttattc aagcactaaa ggaagaaggc gtagaagaag tagaagtcga
     5641 ctcatttgac cacaactttc atatggcagt tcagactctc ccagccgatg atgaacatcc
     5701 agccgatagc attgcagaag tctttcaaaa gggatataaa ttgcacgagc gtctgctaag
     5761 accagcgatg gtagttgtct ataactaagg taaatgagtt ttcgtttttg tccgtaatga
     5821 cagtaaacta gatagcaagt tagaagctat tcagcttgct gattaaacta tagtgattgc
     5881 ttagaattgg aagtaaaata attcgagtgc ttactaagat aaattgaaat aaaaagtaat
     5941 aaagtattat aaaataagag gtattaacat gtctaaaatt attggtattg acttaggtac
     6001 aacaaactca gcagtagcag ttcttgaagg gactgaatca aaaatcattg ctaacccaga
     6061 aggcaatcgt acaactcctt cagtagtatc attcaaaaat ggtgaaatta tcgtgggtga
     6121 tgctgcaaaa cgtcaagcgg taacaaatcc agatactgtt atctctatca aatcaaagat
     6181 gggaacttct gaaaaagttt ctgcaaatgg taaagaatat actcctcaag aaatttcagc
     6241 aatgattctt caatacctta aaggttatgc tgaagactat cttggagaaa aagtagaaaa
     6301 agcagttatt actgttccag cttacttcaa cgatgcacaa cgtcaggcaa ctaaagacgc
     6361 tggtaaaatt gcaggtcttg aagtagaacg tatcgttaac gaaccaacag cagccgcact
     6421 tgcttatggt atggacaaga ctgacaagga tgaaaaaatc ttagtttttg accttggtgg
     6481 tggtacattt gacgtatcaa tccttgaatt aggtgatggt gtcttcgacg ttcttgcaac
     6541 agcaggtgat aacaaacttg gtggtgacga ctttgaccag aaaattattg atttcttggt
     6601 agaagaattc aagaaagaaa atggtattga tctttctcaa gacaaaatgg ctcttcaacg
     6661 cttgaaagat gctgctgaaa aagctaaaaa agacctttca ggtgtaactc aaactcaaat
     6721 ttcattaccg ttcatcactg ctggttctgc tggtcctctt cacttggaga tgagcttatc
     6781 acgtgctaaa tttgacgatc tcactcgtga ccttgttgaa cgtacgaaaa ctccagttcg
     6841 tcaagctctt tcagatgcag gcttgtcatt gtcagaaatt gatgaagtta tcctcgttgg
     6901 tggatcaaca cgtatcccag cagttgttga agctgtaaaa gctgaaactg gtaaagaacc
     6961 aaataaatct gttaaccctg atgaagtggt tgctatgggt gctgctatcc aaggtggtgt
     7021 tatcactggg gatgtgaaag acgttgtact tcttgacgta acaccattgt cacttggtat
     7081 cgaaacaatg ggtggtgtct tcactaaatt gatcgaccgc aacacaacaa tcccaacatc
     7141 taaatcacaa gtcttctcaa cagcagcaga caaccaacca gccgttgata tccatgttct
     7201 tcaaggtgaa cgcccaatgg cagcagataa caaaacactc ggtcgcttcc aattgactga
     7261 tatcccagct gcacctcgtg gaatcccaca aattgaagta acatttgata tcgataaaaa
     7321 tggtattgta tctgttaaag ctaaagatct cggtactcaa aaagaacaac acattgttat
     7381 ccaatctaat tcaggattaa ctgatgaaga aattgataaa atgatgaaag atgctgaagc
     7441 aaatgctgaa gcagatgcaa aacgtaaaga agaagttgat cttaaaaatg aagttgacca
     7501 agccatcttt gcaacagaaa aaactattaa agaaactgaa ggcaaaggct ttgatacaga
     7561 acgtgatgca gcgcaatcag cacttgatga gttgaaaaaa gctcaagaat caggtaacct
     7621 tgacgacatg aaagctaaac ttgaagctct taacgaaaaa gcacaagctc ttgcagttaa
     7681 actttacgaa caagcggctg cagcacaaca agcagctcaa ggggctgaag gtgcacaatc
     7741 agctgattca tcaagcaagg gtgatgatgt tgtagatggc gaattcactg agaaataatt
     7801 attaatattg ttcagattca tttgaatata agcatgaaaa ctatactagc atagtaaagt
     7861 tcttcgtgat aggggttgct caataatcta gataagtttc agattacata agctaatttc
     7921 gctatcacta aataaaaaca tattaataat aaataggcgg ggcgcctcgc tccgtctgtt
     7981 ttattaagtg tcatatatat gttaactatt tagagctgta actgggcaag aataattgtt
     8041 aatctcttca agtgtagtat atgaacaaaa tataaaggat tagataatga acaatacaga
     8101 attttatgat cgtcttggcg tttcaaaaga tgcttctcag gacgaaataa aaaaagctta
     8161 ccgtaggatg tctaagaaat accatccaga tattaataag gagacgggag cagaggaaaa
     8221 atataaagag gttcaagaag cttatgaaac cttgagtgat acacaaaaac gtgctgccta
     8281 tgatcaatat ggcgctgctg gggcaaatgg cggttttggt ggcttcgatg gtggcggttt
     8341 tggtggcttc gatggtggtg gctttggtgg ttttgaagac atcttctcaa gcttttttgg
     8401 tggaggaggg atgcgcaatc ctaatgcacc acgtcaagga gatgacctac aatatcgtgt
     8461 taatttaagt tttgaagaag ctatctttgg agctgaaaaa gaagtatctt ataatcggga
     8521 gtcatcatgt catacttgtt caggaagtgg agctaagcca ggtactagcc ctgtgacttg
     8581 tcaaaagtgt catggttcgg gtgtgattaa tgttgataca caaacacctc taggaacaat
     8641 gcgtcgccaa gtgacttgtg atgtttgtca gggtagtggt caagaaatca aagagaaatg
     8701 tccaacatgt catggaactg gtcatgagaa gaagacgcat aaagtatcag ttaaaatacc
     8761 tgcaggagtt gaaactgggc aacaaattcg tttaacagga caaggtgaag ctggcttcaa
     8821 tggtggtcct tatggtgatc tctttgttat tattaacgtt ctcccaagcc aacagtttga
     8881 gcgtaatgga tcaacaattt attatacttt gaatattagc tttgtccaag ctgctttggg
     8941 tgatacaatt gatataccta ctgtccatgg agcggtagaa atgtcaattc ctgctggtac
     9001 tcagacagga aaaacgtttc gtcttcgcgg aaaaggtgcc cctaaacttc gagggggtgg
     9061 acaaggtgat cagcatgtta ctgttaacat cgtaactcct actaaattga atgatgctca
     9121 aaaagaggca ttgcacgctt ttgcagaggc aagtggagat aagatggtac atccgaagaa
     9181 gaaaggtttc tttgataaag tcaaagatgc tttagatgtt gattaaaaaa taaaagtcta
     9241 gaatcgaaag tattctagac ttttttgtat tgctagaggg attgcttgaa cctaaatttt
     9301 acagtacaat gaatctaata ttgtaggggg acaattttta tggtaaccaa agttgaagag
     9361 attcgctcat atttgatagc ttctatacaa aatggtaagt tggctccagg agatcgccta
     9421 ccatctataa gacagttagc taatcaattt tcctgtaaca aagatacagt gcaacgagtt
     9481 ttgatggaat tgcgttttga taattatatc tatgcaaagc ctaggtcagg ctattacgtc
     9541 tttgattctc atcaagagga agttgaagaa ggggttagtt taccaaactc tgagattgca
     9601 aatatagctt atgatgattt tagattgtgt ttgaatgaga cccttattgg tagggaagat
     9661 taccttttca attattacta tcgtcaagaa ggtcttcttg atttaagtaa agcagtggct
     9721 aaattaatgg aagaaacagg ggtctatgtt ccccttgatg atattgttat tacggctggt
     9781 actcaacagg cattatttat tttgacacag gttacctttc caaatcgaaa atctcgagtt
     9841 ttaatagaag aaccgaccta tcctcgtatg attgaactaa tcaaaacaca aaatttaccc
     9901 tatgaaacta tttctcgagg tactcatgga attgattttc agcgtttaga ggagattttc
     9961 cagacacaat caattaagtt tttttatgtt atacctcgca tgcataatcc tttgggaaca
    10021 tcctataatc cggtagagat gaaaagatta atagagatgg cagagaagta tgatgtttat
    10081 attgtggaag atgactatat gtctgatttt gcaagtcagt caccattaca ttattatgat
    10141 actcacgggc gtgttattta tctaaaatct ttttcaaagg ctattttccc tgctttaaga
    10201 ttagctgcga tttgtttacc acaagcttta aaatcaacat ttatggctta caagaagttg
    10261 atggattatg atactaatct gattttacaa aaagcattag cgctttatat tgaaaatggc
    10321 ctttatgcta agaatagtca atatttgaaa tatcgttatc agaaagacct tgcaaattca
    10381 aaatctattt tagctgatca ccctaatcta ccctcatata gtttacatca cgatagtgta
    10441 ttatttgatt gttcgaaact cgataacttt aaaatattac ggcaatacgg cgatactttg
    10501 gaaaattatt tttgtcaaaa atcgcatcaa tctctcttac aagtaaaaaa tgattcctgc
    10561 ttaaagcagt tcttgggatc gttgtagtat aattgtaggg ggacagttta aaatagataa
    10621 tatagttttc ataaatgaaa ggtggtaaaa taaacccatg acaaggtata aggcacagat
    10681 ttcttatgat ggttcagctt tttcaggctt ccaaagacaa ccaaattgta ggacagttca
    10741 agaagaaatt gagagaactc ttaagcgcct aaatagcggg aacgatgtga taatccatgg
    10801 tgctggtcgc acagatgttg gtgttcatgc ttatggacaa gttatccatt ttgatttacc
    10861 acaggcgcgt gatgtagaaa aattacgctt tggtttagat acacagtgtc cagacgatat
    10921 tgatattgtc aaagtagagc aagtgtctga tgattttcat tgtcgatatg ataaacacat
    10981 taaaacttat gagtttttag tggatatcgg taggccgaaa aatcctatga tgagaaacta
    11041 tgcaacacat tacccttatc ctgtcattat tgagcttatg caagaagcta ttaaagattt
    11101 agtaggtacc catgacttta caggctttac tgcttcaggt acgagtgtag aaaataaagt
    11161 acgtactatt tttgatgcga aaattcagtt tgaagcgtct aaaaatcttt taatttttac
    11221 ctttacagga aatggttttt tgtataaaca ggtgcgtaat atggttggta ccctgctaaa
    11281 aattggtaat ggacgcatgc ctattagcca aatcaaaaca attttacagg ctaaaaatcg
    11341 ggatttagca ggtccaactg cggcaggaaa tggtttatat ttgaaggaga ttatttatga
    11401 agacgaggaa tgttttagca atttcaggaa atgatatttt ctctggcggc ggcttgcatg
    11461 cagatttagc aacttacgtt gttaataaat tacatggttt tgtggcagta acatgcttaa
    11521 cagcaatgtc agataaaggg tttgaagtca ttcctataga ggcatctata ctaaagcagc
    11581 aactagaaag tttaaaagat gtagagtttg ggtctattaa actaggattg cttcccaatg
    11641 tagaaacagc acaagttgtt ttggaatttg taaaatcaaa acaagaatgt ccagtagtct
    11701 tagacacagt cttagtttgt aaagaaaatc atgatttaga agttagccaa ttacgtgagc
    11761 agctaattgc gtttttccca tatgctgatg tgattactcc taatttagta gaggctcaat
    11821 tattgacggg tctttcgatt gaaaacttag atcaaatgaa aattgctgct gagaaacttt
    11881 acgatatggg agctaaacat gttgttatca aagggggcaa ccgtttaaat gctgaagagg
    11941 ctacagacct ttactatgat ggtgaacgtt ttgaaaccta tgtttttcct gtagtagatg
    12001 ccaataatac aggtgctgga tgtacatttg cgtcaagtat tgccagccaa ttagctatgg
    12061 gtaaaaatgt agaagatgca gtcaaaatgt ctaaaggttt cgtctatcaa gctattaaag
    12121 catcggataa gtacggagtt gtccaacatt tttaggagat tttaatgagg aaagagaaaa
    12181 ctagtcaatt ggttcaattg gcaattgtaa cagctatatc tattgtattg gggatgttta
    12241 taagtattcc gacaccaaca ggttttttga cattactaga tgctggaata ttctttgctg
    12301 ccttttactt tggcaaaaaa gaaggggctg tggtaggtgc cttagctggt tttttaattg
    12361 atttattgaa aggttatccc aattggatgt tctttagttt attgattcat gggacgcaag
    12421 ggtatttagc gggtttacca ggtagaagac gtcttttagg tcttattagt gcaactcttg
    12481 ttatggtatt aggttatgct atagctagtg gattgatgta tggttgggga gctgttctac
    12541 cagatattcc tggcaatatt atgcaaaata tggttggtat ggttgttggt tttgctctta
    12601 ataagtctct agaaagagtg aagaaatgat actagattat aataagttaa agcaggaaac
    12661 taaagctatt gtagttgata ttattgaacg tagcgctctt aaaaaaggtc aaatttttgt
    12721 actaggtcta tcatctagtg aggtttctgg tggtttaatt ggaaaaaatt ctagttctga
    12781 aattggtgaa attatcgttg aagttatcct caaagagtta cattcacgcg gcatctattt
    12841 agcagttcaa ggttgtgaac atgttaatag ggcgttggtt gttgaagcag aattggctga
    12901 acgtcaacag ttggaagtcg ttaatgttgt tcctaatcta catgctggcg gtagtggtca
    12961 ggttgcagct tttaagttga tgacatcacc agttgaggtt gaagaaattg tggcacatgc
    13021 tggaattgat attggagata cttctattgg aatgcatatc aagagagtac aagttccctt
    13081 gattccaatt agtcgtgagt tgggaggagc acacgttaca gcactagcta gtcgtcctaa
    13141 attaattggc ggtgctagag caggatatac gagcgatcca atccgtaaat tttaaaataa
    13201 acctctgctc tgcagaggtt tattatttat ctcaacgaga gtggttattg gagaaattgt
    13261 acgttggtaa gtcaatacct tcttcaagaa tagctttttg ataaagtttg taaaattcag
    13321 catagatgtc aaactgagca ccgttttgcg tgaagatatt aatacgataa acaaattgac
    13381 cattagcatt ggttgtagga ccaaaaacag taggctctcc aacgatagca gggtgttttg
    13441 aaacatactc ttcattgatt tttgttacaa tatcagaaat ctgatcaagg tttgtatgaa
    13501 caaataaagg aatatcaatt tgagcacgca tattgctacg agacttattg ctgacaattg
    13561 taatattacg attaggaatg aagtgcaagg ttccatcgaa atcatgaatt tgtgtggtgc
    13621 gtatcccaac attagtaaca gtgcctgata cagtaccaac attaataata tcaccaacat
    13681 caaattggtt ttctagcaga ataaaaaagc cattgacaac atctgataag aagccttgag
    13741 caccaagtcc aatagcaacc ccagcaatac cagccccagc taataaacta gaaattggaa
    13801 cgcccaatat acttaaaatc caatagaata gaaagaaata aagggtatag tttaaaacat
    13861 tgtgagaaag tttagctaat gttttttgac gagctacttt ttgtctagaa aaggcgagtg
    13921 agcgattaac agtcttttcg aaaagataat tgatgacttg cctaacgata acgaatgcaa
    13981 tgattaataa taagattgat attagtttgg tgaagaaagt aaataaaact tcctctacat
    14041 taagatggtc tataaatttt tctaaagtaa tcatagtaat agtctagtaa aaaaaaagcc
    14101 atagagcaag tattttaaaa gcttatcgtt aagcagttta aagcttcttt tttgcataaa
    14161 aactttgtaa aacgttttaa attatggtaa actaaactgt atctaaataa ttttaaggag
    14221 aaatgactta atgtctacat catttgaaaa taaagctaca aaccgtggta ttatcacatt
    14281 tacaattagt caagatgaga tcaagccagc tcttgatcaa gcattcaaca aagtaaaaaa
    14341 agatttgaac gttccaggat tccgtaaagg acatatgcct cgtacagttt tcaaccaaaa
    14401 atttggtgaa gaagcactct acgaaaatgc attgaattta gtgttgccaa aggcttacga
    14461 agcagctgtt gcagaacttg gtttagacgt tgttgctcaa ccaaaaattg atgttgtatc
    14521 aatggaaaaa ggtcaagatt ggaagttgac agctgaagtt gttacaaaac cagaagttaa
    14581 acttggagat tacaaagacc tttcagtaga agttgatgct tcaaaagaag tttcagatga
    14641 agaagttgat gctaaagttg aacgtgagcg taacaacctt gcagaattga ctgttaaaga
    14701 tggtgaagca gcacaaggtg atacagttgt tattgacttt gtaggttcag ttgatggtgt
    14761 tgaatttgat ggtggtaaag gagataactt ctcacttgaa cttggttcag gtcaatttat
    14821 cccaggattt gaagaacaat tagtaggttc taaagctggt caaactgtag acgttaatgt
    14881 aacattccca gaagattatc aagcagaaga tcttgctggt aaagatgcta aatttgttac
    14941 aacaattcat gaagtaaaaa ctaaagaagt tccagcactt gatgatgaac ttgcaaaaga
    15001 tattgatgat gaagttgaaa cacttgatga gttgaaagcg aaatatcgca aagagctaga
    15061 atcagcaaaa gaaattgcat ttgatgatgc agttgaaggt gcagcaatcg aattagctgt
    15121 tgccaatgca gaaattgttg aattaccaga agaaatggtc catgatgaag tacatcgtgc
    15181 tatgaatgag ttcatgggta atatgcaacg tcaaggaatt tcaccagaaa tgtacttcca
    15241 attaacaggt acaactgaag aagaccttca taagcaatac caagcagatg ctgacaaacg
    15301 cgttaaaact aaccttgtta tcgaagcaat tgcagctgca gaaggctttg aagcaacaga
    15361 tgaagaaatc gaaaaagaaa tcacagatct tgcttcagaa tacaatatgg aagctgacca
    15421 agtacgcggt ctactttcag cagatatgct taaacatgat attgcaatga aaaaagcggt
    15481 tgacgttatc actagctcag ctactgtaaa ataattgctt attaaatttt gaaaagattg
    15541 ggcttcccag tctttttatt tgtgattatt cttttgacga atagctaaaa atagcgtaaa
    15601 atagtaaata gcgaaaattt gaaaatatga cttatctatt ttctatctat aaagaatatt
    15661 gctaaacaaa atgatagtct tacacaatta aggagaataa ctttggaatt agaagtattt
    15721 gctgggcaag aaaaaagtga actttcgatg attgaagttg ctcgcgctat tttagagcaa
    15781 cgtggtcgtg ataacgaaat gtacttcagc gatttggtaa atgatattca aacctacctt
    15841 ggaaaatctg actcagcaat ccgtgagtca ttgccatttt tctattcaga tttgaataca
    15901 gacggaagtt ttattccact tggcgaaaac aaatggggac ttcgttcatg gtatgctatc
    15961 gatgaaattg acgaggaaat catcactctt gaagaagatg aagatggcgc tccaaaacgt
    16021 aagaagaaac gtgtcaatgc tttcatggat ggtgatgaag atgctattga ttacaatgat
    16081 gatgatccag aagatgaaga tttcacagaa gaaacaccaa gtttagaata tgatgaagaa
    16141 aaccctgacg atgaaaaatc agaggtagaa tcgtatgatt ctgagattaa tgaaatcatt
    16201 cctgatgaag atttagatga ggatgtagaa atcaacgaag aagacgatga agaggaagaa
    16261 gaggaagaag aggtttagac ggggattttg tatttgacaa atagtcagat atagtttaaa
    16321 ctattattcg ggcacctctt atgaggtcag ataaagctcc ctagtgatag ggggctattt
    16381 tgtttttggg aaagatacag aatggttcca gatagttttc tgagtaggcc cttgatcaga
    16441 tagtttctac tagttttgac tagagaagct ggtattattt atcaatcatg tgttttgggt
    16501 tttacccgta aactaactgt tagattaaca tttaatttaa ggagttttta atgacaaagt
    16561 atatttttgt tacaggtggt gtggtctctt ctattggtaa ggggattgtt gcagcaagtc
    16621 ttgggcgctt actaaaaaac cgtgggctaa aggtgacaat tcaaaaattt gatccatata
    16681 ttaatattga cccagggaca atgagccctt atcaacatgg agaagtttat gttacagatg
    16741 atggtgcaga aactgacctt gaccttggac actacgagcg ttttattgat attaacttaa
    16801 acaaatattc aaatgttaca acgggtaaga tttatagtga agttcttaaa aaagaacgcc
    16861 gtggagaata ccttggtgca actgttcaag ttatcccaca cgttacagat gctttgaaag
    16921 aaaaaattaa acgtgctgcg acaactacag attcagatgt tattattact gaagttggcg
    16981 gtactgttgg agatattgag agccttccgt ttttagaagc acttcgtcaa atgaaggcag
    17041 atgttggttc tgataatgtt atgtacattc atacaacgtt attaccatac ctaaaggctg
    17101 ccggcgaaat gaaaacaaaa ccgactcaac attctgttaa agagttacgt ggcctaggta
    17161 tccaacctaa tatgcttgtt atccgtacag agcaaccagc aggtcaaagt attaagaata
    17221 aattggcaca attttgtgat gttgcaccag aggctgttat agagtccctt gacgttgacc
    17281 atatttatca aattccactt aacatgcaag ctcaaaatat ggatcaaatt gtttgtgatc
    17341 acttgaagct tgagacacct gctgctgata tgacagaatg gtcagcaatg gttgataaag
    17401 taatgaacct tgagaaaaaa gttaaaattg cattggttgg taaatatgtt gaattacctg
    17461 atgcctacct ttcagttgtg gaagccctta agcactcagg gtacgtcaac gatgttgcta
    17521 ttgatctaaa atgggtaaat gctgctgaag tgacagaaga taatattaaa gaacttgtcg
    17581 gagatgcaga tggtatcatc gttcctggtg gatttggcca aagaggtagt gagggtaaaa
    17641 ttgaagcaat tcgctatgca cgtgaaaatg atgttccaat gcttggtgtt tgtctcggaa
    17701 tgcagctaac ttgtgtcgaa tttgcgcgta atgttttaaa ccttcatgga gctaattcag
    17761 cagagcttga ccctaaaaca ccgttcccaa tcattgatat catgcgtgat caaatcgata
    17821 ttgaagatat gggtggtaca cttcgccttg gtctatatcc atgtaaattg aaatcaggtt
    17881 caagagctgc ggcagcttat aataatcaag aagttgtcca acgtcgtcac cgtcaccgtt
    17941 atgaatttaa tacaaaattc cgtgagcaat ttgaggctgc tggatttgtc ttctcagggg
    18001 tatcaccgga taatcgtttg atggaagttg ttgagttacc agagaaaaaa ttctttgttg
    18061 cagcacaata ccatccagaa ttacaatcac gtcctaacca tgctgaagag ctttacacag
    18121 cttttgtaac cgcagcagtt gaaaatatga aataacttta tcgcctccca gtattaggag
    18181 gcgattttat ttttttagat agtttcacta tcaaagtatc attagctctt ttctttttct
    18241 aaaaataagg tataataggt tgcatgaaaa agattcgatt atcaaagttt attaaaatga
    18301 ttgttgttat tttgttttta attagtgtag cagctagttt ttattttttc cacgttgccc
    18361 aagttcgaga tgataaatcc tttatttcaa atggtcaacg taagcctgga aactctttat
    18421 atgcttatga taaatccttt gataagctat taaagcaaaa aatagaaatg acaaaccaaa
    18481 atataaagca agttgcttgg tatgttcctg ctgttaagaa aactcataag acagctgttg
    18541 tcgttcatgg ttttgcgaat agcaaagaga atatgaaggc atatggttgg ctgtttcata
    18601 agttaggata caatgttctt atgcctgaca atattgcaca tggtgaaagt catgggcagt
    18661 tgataggcta tggctggaac gaccgcgaga acattatcaa atggacagaa atgatagttg
    18721 ataagaatcc atcaagccaa attactttat ttggtgtttc aatgggtgga gcaacagtca
    18781 tgatggctag tggtgaaaaa ttacctagtc aggttgttaa tatcattgaa gattgcggtt
    18841 attctagtgt ttgggatgaa ttaaaatttc aggctaaaga gatgtatggt ttaccagcct
    18901 tcccactctt atatgaagtt tcaacaattt ctaaaatcag agcaggtttt tcgtatggac
    18961 aagcaagtag tgtcgaacaa ttgaaaaaga ataatttacc agccctcttt attcatggtg
    19021 ataaggataa ttttgttcca acaagtatgg tttatgacaa ctataaagct acagcaggta
    19081 agaaagagct ttatattgta aaaggggcaa aacatgcgaa atcttttgaa acagagccag
    19141 aaaaatatga gaaacgtatc tctagttttt tgaaaaaata tgaaaaataa tattgacaac
    19201 aatatgataa gttgatataa tttattatgt tgttaagcgg ggatggcgga attggcagac
    19261 gcgcaagact aaggatcttg tgaccgcttt tggtcgtgag ggttcaagtc cctctctccg
    19321 cagactagtc gagttcattt ggaactcgac tttttgcata tttaagataa agatggagta
    19381 tcacatgtca aaagtacgtg gctttgaatt agttagccaa tttagcaata aagagttatt
    19441 accaaaacga gaaacagctc acgcagctgg ctatgattta aaagtagcta aaaaaacggt
    19501 tattgaacct ggtgaaatta cattggtccc gaccggtatt aaagcataca tgcaaccagg
    19561 ggaggtcctg tacctttatg atcgttcatc gaatccacgt aaaaaaggga ttgtcctaat
    19621 aaattcagtt ggtgttattg atggtgacta ctataataac caagtcaatg aaggacatat
    19681 ttttgcacag atgcaaaata ttactgatca ggctgttatt ttagaagaag gggaacggat
    19741 tgttcaggct gtttttgccc cattcttatt ggcagatgac gatcaggcta caggaatgcg
    19801 tacaggtggt tttggtagta ctggaaaata aaattatttt acgaataatg ttagaaaagg
    19861 ctagacttac ttcttagcct tttttgatga gagtaaactt atattttctc taacttggtg
    19921 tgatataatg agtgtactag ttatttggta ccaagctatc atattaattt agaagttata
    19981 aaggaggcct gagtggctaa gaaaaaatca gtcttcacct gtcaagagtg tggttaccaa
    20041 tcacctaagt atttgggacg ttgtccaaat tgctcagcct ggtcttcatt tgtagaagag
    20101 gttgaagttc aagaagttaa aaatgcacga gttagtttga acggtgaaaa gtcccgaccg
    20161 acgaagttaa aagatgtttc ctcaattaac tattctagaa ctaagactga tatggatgaa
    20221 tttaaccgtg ttctaggagg cggtgttgta ccaggaagtt tggttctgat tggaggagac
    20281 cctgggatag gtaaatctac tttattacta caagtatcaa ctcagctagc taataaaggt
    20341 acagtattat atgtctcagg tgaggaatct gcagaacaga ttaagttacg aagtgagcgt
    20401 ctaggcgata tcgataatga attttacctt tatgccgaga ctaatatgca aagtattcgg
    20461 tcggagattg agaagattaa accagatttt cttattattg attctataca aactattatg
    20521 agtcccgaag tatcaagtgt ccaaggctct gttagccaag ttcgcgaagt gaccgctgaa
    20581 ttgatgcagt tagctaaaac taataatatt gctactttta ttgtaggtca tgtgactaag
    20641 gagggaacat tagcaggccc tcgaatgcta gaacatatgg tggatacggt tctttatttt
    20701 gaaggggagc gtcatcacac gttccgtatt ttacgggcag tcaaaaaccg ttttggttcg
    20761 accaatgaaa taggtatctt tgaaatgcag tcgggtggtc tagttgaagt tttgaatcca
    20821 agtcaagttt ttcttgaaga acgtttagac ggtgcaacag gatctgctat tgttgttact
    20881 atggaaggaa caaggcctat tttagcagag gttcaagctt tagtgactcc gactgttttt
    20941 ggaaatgcta agcgtacaac aacaggtctt gactttaatc gtgtcagtct tattatggca
    21001 gttctagaaa aacgttgtgg tttattgctt caaaatcaag atgcctattt aaaatcagcg
    21061 ggtggtgtta aattggatga accagcaatt gatttagctg ttgctgtagc tattgcttcc
    21121 agttacaaag aaaaacccac aaatccacaa gaatcattta ttggagaaat tggactcact
    21181 ggtgagattc gtcgcgtgac acgtattgag caacgtatta atgaggctag taaattgggc
    21241 tttactaaaa tttacgcacc aaagaattct ctagctggga ttgaaatacc taaaggcatt
    21301 gatgttattg gtgtgacaac tgttagtcag gtcttaaaag cagtgttttc aacataatga
    21361 ttagattcgg ttcagccgaa tcttttttgg tggcgagaaa catttcgtga aaaataatgg
    21421 gcaaaatgat aatataaaag ggttggtatt tagtaaatat cgaatgatgt tacactaaca
    21481 aaggagatct ctatgaccac ttattttgat aactttttaa aaaccaatca agcctatgct
    21541 gacttacatg gaacagctca tctacctatc aagccaaaga caaaggttgc tattgtaacc
    21601 tgtatggatt ctcgtttaca cgttgctcaa gctctagggc tagctttagg agatgctcat
    21661 attttacgta atgctggtgg acgagttaca gacgatgttc tacgttcttt agttatttcg
    21721 caacaacaat taggaacacg agaaatcgtt gttctacacc atacagattg tggagcacag
    21781 acgtttacta acgaagcttt tgctgctcaa ttacagagag atttgggtgt tgatatgcat
    21841 ggacatgatt ttcttccttt taatgatatt gaagaaagcg ttcgtgaaga tgtggcaaaa
    21901 ttacatgctt ctccattaat cccagatgat gttgttattt ctggtgctat ttatgatgta
    21961 gatactggtc gtatggtgga agttaaatag aaaaaatcct gtccttagcg atgggttttt
    22021 ttattttaga aaactagatt taaaggcaac agttaggctt atcatgcctt atgctggaat
    22081 tcagacagag gaaacatctt ttgctatact gaaactatac tataaaagcg gaggaaattt
    22141 aaaatgaaaa aatatgatgt cattgttctt ggttttggta aagcaggtaa aacattagca
    22201 gcaaaattag caacacaagg taaaagcgtt gcaatggtcg aagaagacga taaaatgtac
    22261 gggggaacat gtatcaacat tggatgtatt ccgacaaaaa cgcttttagt atcagctagc
    22321 aaaaatcatg attttcaaga agctatgact actagaaatg aagttacaag tcgtttacgt
    22381 gctaaaaact ttgctatgtt ggataataaa gacactgtgg atgtttataa tgctaaagcc
    22441 cgctttatat caaataaagt ggtcgaatta acaggaggag ctgacaaaca agaacttaca
    22501 gctgatgtta ttattatcaa tactggtgct aagtcagtac aattaccaat cccaggatta
    22561 gctgattctc aacatgttta tgattcaacg gcaatccaag aattggctca tcttcctaaa
    22621 cggttaggta tcattggagg cggtaatatt gggcttgaat ttgcgacttt atattccgaa
    22681 ttagggagca aagtgacagt tattgatagt caatcccgta tctttgcgcg tgaggaagag
    22741 gaattatcag agatggctca agattattta gaagagatgg ggatatcttt taaattatca
    22801 gccgatatca aatctgtgca aaatgaagat gaagacgttg ttattagctt tgaggatgaa
    22861 aaattaagct ttgatgcagt attatatgca acaggacgta agccaaatac agaaggttta
    22921 gcacttgaaa atacagatat taaattgaca gaacgtggag ctattgctgt tgatgaatat
    22981 tgccaaactt ctgttgagaa tatttttgca gttggtgatg ttaatggcgg ccctcaattc
    23041 acatatattt ctttagatga ctcacgtatt gtcttaaatt atttgaatgg tgataaggat
    23101 tattctctaa aaaatcgagg agcagtaccg acttcaacct ttacaaatcc tcctttagca
    23161 acggtaggtc tagatgaaaa gaccgctaag gaaaaaggat atcaagttaa atctaatagc
    23221 cttcttgttt ctgccatgcc acgtgcacat gttaataatg accttagagg tatttttaaa
    23281 gttgtggtag atactgagac aaatcttatt ttgggagctc gcttatttgg tgcagaatcc
    23341 catgaattaa ttaatatcat cacaatggca atggataata aaatcccata tacttatttc
    23401 caaaaacaaa ttttcactca cccaacaatg gtagagaatt ttaatgattt gtttaacttt
    23461 taagaatatt tttaaaaagt aaggcaattt tgctttactt tttttatcga aaattagtgt
    23521 ggctattatt actgattatc atttttagtg atttagtggt ataatagggt tgattggcaa
    23581 tcatctgtaa gagtttgaat gatgttttgc ccccttgact aattcagtta aactgaaaaa
    23641 gtttactatc agaaaatata ggagtagaat tttggctaat aagattcgtg tgcgttatgc
    23701 accaagtcca acaggattgt tacacatcgg aaatgcgcgt actgcgctct ttaactacct
    23761 ttatgctcgc catcatggtg gggattttgt tattcgtatt gaagatactg accgaaaacg
    23821 ccacgtcgaa gatggtgaac gctctcaatt ggagaactta cgttggctag gtatggactg
    23881 ggatgaaagt cctgaaacac atgaaaatta tcgtcaatca gaacgccttg agctctacca
    23941 acgttatatt gatcagttgt tagcagaagg taaagcctac aagtcctatg tgactgagga
    24001 agaattagca gcagagcgtg aacgccaaga attggcaggc gaaacacctc gctatatcaa
    24061 cgaatttatc ggaatgtcag aaactgaaaa agaagcttac attgcagagc gtgaagcagc
    24121 aggcatcatt ccgacagtac gccttgctgt taatgaatct gggatttata aatggactga
    24181 tatggtaaaa ggtgacattg agtttgaagg ctctaatatc ggcggagact gggttattca
    24241 gaagaaagat ggttatccaa cttacaattt cgcagtagtc attgatgatc atgacatgca
    24301 aatttcgcat gttatccgtg gcgatgacca tattgcaaat acgccaaaac aattaatggt
    24361 ttatgaagca ctcggttggg aagcgccaca atttggtcac atgacattga ttataaactc
    24421 agagactggt aagaaattat caaaacgcga caccaatact cttcaattta tcgaagacta
    24481 tcgtaaaaaa ggttatatgt cggaagctgt ctttaatttc attgcccttc ttggctggaa
    24541 tcctggtgga gaagaagaaa tcttctcacg cgagcaatta attaatctat ttgatgaaaa
    24601 tcgtcttagc aaatcaccag cagcattcga tcagaagaaa atggattgga tgagtaatga
    24661 ttacctaaaa aatgctgatt ttgagagtgt ttttgcactt tgcaaaccat tcttagaaga
    24721 agctggtcga cttacagaca aagctgaaaa attagttgag ctttatcaac ctcaattaaa
    24781 atcagctgat gagattgtac cattaacaga ccttttcttt gcagattttc cagaactaac
    24841 agaagccgaa aaagaagtaa tggcagctga aacagtccca acagtcttat ctgcttttaa
    24901 ggaaaagtta gtatcgcttt ctgacgaaga attcactaga gatactattt tccctcaaat
    24961 taaggctgtt caaaaagaaa caggtattaa aggtaagaac ctatttatgc ctattcgtat
    25021 tgcggtttct ggtgaaatgc atggaccaga gttaccagat actatttatt tattaggtaa
    25081 agaaaaatca gttcaacata tcgataatat gttagcaaaa ttataatata aaaagtcgtg
    25141 aattttcacg actttttgtg tacttttacc aattgtattt ttcaacattg tctttggtaa
    25201 caaggtaaat tggagaaatt gtttctttct ctactttttt gcctttataa taatcaattg
    25261 ctgcttgaat tgctatttct cccatcttag ctggttgttg tgcaatagtg gctgaaatat
    25321 ctcctttttt aatggcatca tgggcatctg gctgaccatc aattcctacg ataagaacat
    25381 tttgcaagcc agcagatttg acagcctgtg cagccccaag tgccatttca tcattttgtg
    25441 cgaaaataat ttggacatct ttatgtcctt gaatcatatt ttgtgtagta ttcaaagctt
    25501 tggcgcggtc aaaattggca gattggcttg aaaggatgtc aagtttagat tttgcaactg
    25561 aatgaaatcc tttaccacgg tctacagtag cagaggcacc tggtactcca gataattcaa
    25621 aggcttttgc ttttttgcct agttttttaa cggcataatc cgcagccatc ttaccagcag
    25681 caacgttatc tgaagcgaca gtagttaata ctttgcctcc ctcactacca cggtccatta
    25741 gaataacagg aatattagcg ttgtttgcag acttaatagc tgttacaata gcttttgagt
    25801 caacgggatt aatcaaaata gcatctacgt tttgactaat aaagttttgg acatcatctg
    25861 cttgacgtgc ggcgtcatct tgagcatcag ctaccttaat gcttattttt ttattgctag
    25921 catatttgtc aataccgtct ttcatcgcaa caaagtaggg attgttagtt gttgaaatag
    25981 atacacctag tttcaaatct ttagcagatt ttttggttac attttttgta gaattcccag
    26041 aagaatttcc aagtccagtt ttaccacatg cacctagaat gaggacaata gacatcaata
    26101 aggctagaaa gccaagtttt tttccaaatt tcatttgaaa ctcctttaga taaaatataa
    26161 tgaacttata gaaattagag taaagactat tttgctactt taaagcgatc aagtaaaaca
    26221 gccattaaaa taacgatacc tttcacaact tgttgccaaa atgcagagac accaatgatg
    26281 tttaatccat tgttcaggac accaataata agagcaccaa tcaatgttcc aatgatacgt
    26341 cctttcccac cagataaaga cgttccacct aagacaacgg cggcaatagc atccatctca
    26401 taacttgctc cagctgttgg ttgtgctgag ctaaggcgtg atgtgataat taacccagaa
    26461 atggaagcca tgatacctga gatagtgtag atgataattt taactttgtt taacttaata
    26521 cccgagatat acgctgcttt ttcattacct cctaaagcgt aaacagattt cccgaaggct
    26581 gttttatgta acaaaatata caaaataata aaagtaagaa acatgagaat gactgggaat
    26641 gggataccaa caatatagcc ttgtcccaaa aattggaata gaaaactatc ggataagcca
    26701 gcggtaattg ggttgccgtt tgaataaact agcgttgctc ctctaaagat agtcattgtt
    26761 gccagtgtaa cgataaatgg tgctaatttt ccgtaagaaa tgaagagtcc gtttagcatt
    26821 cccaagatgc cacctaaggc aacagataaa acgatagcaa taccaacggg tactccgtgg
    26881 ccgattaatc cagctgttaa tgcactagat agggctaata tggacccgac tgataaatca
    26941 ataccgccag tcaaaataac gaaggtcatc ccgaaagcga taaaaccatt tgcagttact
    27001 tgtaataata aattgagtag attattggtt gtgaggaaat taggattaat gagtgtgata
    27061 ataaccatta aaccagctaa cgcaatcagg gttgttaact ctgatatata tttcatcata
    27121 tttttcactt tacttacctc ctgtggctag ttgcataact ttttcttggt cagcttcttt
    27181 tcgactgagt tctccactga ttcgaccttc gtgcattacc atgatgcggt cactaacacc
    27241 tagaatttca ggtaagtctg atgataccat gacaattgga acacctctgt ctgctagttc
    27301 attcattaat tggtaaatct ctcgtttagc accgacatca acaccacgcg ttggctcatc
    27361 aagaattaag actttaggag caataccaat ccatttagcg agaacgacct tttgttggtt
    27421 acctccagaa agatttccaa cctctaaatc tggtctccct gatttaatgt agagacgatt
    27481 aatcaattgt tgcacaaatg ttgtgcttgt tttttcatca aagaaaccat gtttgctaaa
    27541 atctttagtg ctagggagag tcatattgtc cttgatattg aagtctagga tgagaccttc
    27601 atcttttcta ttttcggtca aaaaaccaat gccctgcttg atagcttggc ttggggtagt
    27661 aatagtgatt tcttggtcat caattttcac cttaccagac ttctttttat caattccaaa
    27721 gatagttcgc ataacttcag tacgcccagc tcccattaat ccagaaaatc caagaatctc
    27781 gcctttacgg acataaaagg aaacgtcctc gaaattatct ccacagaggt tactaacttc
    27841 aaaagcaaca ggcccaattt cagaatgttt ttcaggatag taatcttcca attttcggcc
    27901 aaccattttt ttaaccaatt cgtcagaatt tgtgagtgat gtttcttttg tatctacaat
    27961 gacaccatct ctcatgacgg ttacgaagtc agtgatttta aaaatttcct ccattcgatg
    28021 ggagatatag acaacgccca ctccttcttg tttcaaacct cggataactc ggaaaagatt
    28081 ttcagtttca cgatctgtta aagcagcagt cggttcatcc attaccaaaa tagagagttg
    28141 gtttaggaga cttttggcga tttcaatcat ttgttgttgt ccaacagaca agttaccaat
    28201 cgggatgtct aggggaatag tcacacctaa tcttttaaaa gtttcaagtg ctttttgtct
    28261 cattaatttt tgattgagta agccaaaagt tgtcttgatt tcacgtccaa gaaagagatt
    28321 ttccaagaca gtcatttccg gccaagtatt catctcttgg tgaataaaac taataccaaa
    28381 ttcctctgct tcttgagggt ttgaaaaagt tcgttcctcg ccatcaatat aaatagttcc
    28441 tgtacttgct ggaaaaagtc ctgttagaat gttcattaag gttgatttac ccgcaccatt
    28501 ttcacccata agcgcgtgta tttgaccaga ttgtaattcc aaatcaatct tttctagaac
    28561 tttattagta ccgaaggatt tggagatatt cctcatgtca attttcatgg actaaactcc
    28621 tttctagatg ataacaccag attgtagaat aatatttgaa tacggtgtat tttcacctgt
    28681 acgaataact gctttgacat cttgggtcat ttgttttaaa tgattgtgac tgacgtactc
    28741 tatgctaaca gagttatcaa gtttagctaa taaccttgat aattgatccg gatttgcttc
    28801 cttgatttct tctgctaaaa tgactttttc aactaagata ttctctaaat agatatcaag
    28861 tacctcttgg aaactaggta ttccagaggt caaagacaaa tcaatctttg gaatgccatt
    28921 tggaacaggt agacccagat ctccaataca aactcgatca gtatgcccaa ggtcgtctgc
    28981 taatttagcg agatgactat ttaatatacc agtctttttc ataatgttgg ttgtcctcca
    29041 tttctttaat tgtaggcatg ccaccctgtg caccaaattt ttgcacggat aagtgagcag
    29101 ctagagttgc aaaacgtaag gccttagcaa tcttaaattt tttcgagata gcataaccga
    29161 atgcaccgtt aaaagtatca cctgcaccag ttgtatcaac agctttagtt tctaaagcag
    29221 gaattaaatg actttctttt ccatcagagt agatggctcc ttttgtgccg agtgttacga
    29281 ttagtttttc aggatatgtg gctaatatat cctctagttt ttggtttggg aacagctctt
    29341 gacattcgtg ttcatttggt gtgaaataat cgaccttatc gagcatctca atatcagtct
    29401 ctctagatgg tgcaggatta tagagtaatt taatactatg ttctttacaa aaattagcaa
    29461 ttttcatatt tgcttgatga ggaatttcat tttgtaatac aactaaatca gcttctttaa
    29521 ttattgacca ctcctgagac cattttttag tatcaacttt tccgttggct ccaggacagt
    29581 aaatgattcg attatcatta ttataaagag tgatttgggc aacacctgaa gaagatggta
    29641 tcgttcccac aaaatccgta gtaacatgat ttttgtttaa attatccaaa agaatgggac
    29701 caaaagaatc ctcgccaatg gcaccaagaa tagtaatatt atctcgctct tggctaagac
    29761 gcccaatagc gacagcctga ttagcacctt tacctcctgg aaccattgaa aaacgttggc
    29821 caaatacggt ttctccctct ttggcgatac gattggtttc cataactaag tccatggaaa
    29881 tactaccgat aatgacaatg ttactcatct attttcctcc ttagggtttc acgttcgaca
    29941 taatgaacgg gtaatttaat acgcttattg gtaataggta attgattagt aattttgaaa
    30001 attaattcag ctgctttttg tcccataata tatgatgatt ggtgaatagt tgacaacgaa
    30061 gggtagataa attggctcat taaaatatca tcataaccaa taatttgtac atcttcaggg
    30121 attcgcttcc ctcgattaag aatctcgtgt aggtaggcaa tagcatgtat atcagatgga
    30181 gcgataatgc tatcaatgtc tggatggtgg tcaagtaaag ttttcgcttc aatttgaatc
    30241 tctgcaaaat caaaactggc actatcttcg atagcaaaac aagccccttt gtttaatagg
    30301 taattttggc ttccttgaaa cctttgatta aggttatcag ctttatctag cggaccacga
    30361 atgagtaata tattggtagc acctgctgtc caaatagctt gagctgctaa ttttcctccc
    30421 tctttattat cagagaaaac gctgtactgt gtttcttgat taacgcggtc aacaactaca
    30481 actggaatat ctatttcagg atgatttttg gtgaaatcgt gtgttgtaat aatccctgca
    30541 gcattacttt gtaatagcac atttaagtac tcttcttcta aatggctctt gttatttgtg
    30601 ttgccaagca tgacacgata accctgttct tttagaaatt cttcaactcc tctagcaagt
    30661 cttggaaaga aagggtttga aatatcggga agtagtaatc caacaagttg attttttttg
    30721 gttttcagag attgtgcaag gaaatttgga acataatgga gttcgcggat agcttgctcg
    30781 attttttgac ttgctttttg actaacgtaa ccattttggg atatatacct tgagacagta
    30841 gatgttgata cgccagcttt ctcggcgact tgtctaatcg tactcataga aactcctttc
    30901 ttatattgtg gaaacggttt cgcaattaga attgtaatcg cttactaaaa aattgtcaag
    30961 ctatttttta aataatttaa tgataaaata tcattatttt tagaggtcat attcaagtca
    31021 taaagctgtg atactatgtt cacatcaaaa gatcaaagga gaaaaaccat gtcacatgct
    31081 tggaaagaat taacctttta taagaaaaaa tacctcctga ttgaattgct aatcattgtt
    31141 atgatgttca tggtagtttt tttatcaggt ttagcaaacg gtttaggtag agctgttagt
    31201 gctgctattg aaaataaccc agctcaaacc tatattttga atgaaggtgc tgaacaagtg
    31261 attacatcat ctgtattgac aactaaagat cagacagatt taaacagttt gaatttaaaa
    31321 gatagtacca ctttaaatat tcaacgttcg agtttaactc gacaaggaca cgaaaagaaa
    31381 attgatattt cttattttgc tattgataaa gattctttta tggcacctac actttctgag
    31441 ggaaaacaac tgacaagtta caaaaaagct attattttaa atgatagttt aaaagctgaa
    31501 gggataaagt taggtgataa agtgattgat aagtcatcat ctatatcact aacagtcgtt
    31561 ggatttgtcc ataatagtat gtatggtcac ggacctgtgg catttattga taaagacatt
    31621 tataccgaaa ttaataaaaa gattaatcct caataccagt tcttacctca ggcattagtt
    31681 atgaaaaatg ataaaagtat atctcattta ccaactcaat tagaagctgt tagtaaaaaa
    31741 gatgtcattc aacatattcc tggttatagt gccgaacaat ccactttgaa tatgatttta
    31801 tgggtccttg tcgtagcttc agctggtatt ttaggtgtct ttttctacat catcacactt
    31861 caaaaacgtc atgaatttag cgtcatgaaa gctattggga caaaaatgtc agagattgcc
    31921 ctatttcaat tgagtcaagt cattatattg gcactgtttg gtatcattgt tggagatgga
    31981 ttggcggttg ctctctctta tgtattacca gctcaaatgc catttgttat taattggcaa
    32041 aatattattc tagtatcgtt tgtattttta gtcattgcaa tgattagtag tgctttgtca
    32101 attgtaaaag ttgctaaaat cgacccagtc gaagttatta atgggggagg agaataatgt
    32161 cagcaatctt agaattaaaa cacatctcaa agcactatcc agatggagat gagcttttat
    32221 caatattaga taatttagat ttatcagttt ctgcaggtga gtttgtagct atactgggac
    32281 cctcaggttc aggtaaaagc acacttttat ccattgcagg gttacttctt ggcgcagatc
    32341 aaggtagttt gtatgtcaat catgagaacg ttacggattt gtctcaaaga caaagaacac
    32401 aattgcgccg tgaagcacta ggttttatat ttcaaagtca tcaactgctt ccttatttaa
    32461 caatccaaga acagttgcaa caagaggctc gatttgcaaa acactatgat aagaaaacaa
    32521 gcttggaaga aattaataaa ttattatcag atttaggaat agagcagtgt gcacataaat
    32581 accctaacca attatcggga ggacaaaagc aacgcgcagc aattgctaga gcatttatta
    32641 atcatcctaa ggttatcttg gcagatgagc caacagctag ccttgatgaa gaacgtgggc
    32701 gtcaagttac agaacttatt cgtcaggaag ttaaatcaca taatactgca gctattatgg
    32761 tgacacacga tgagcgcgtt ctagatttag tagataccgt ttatcgttta aaggacggga
    32821 aattagttaa agaaaacttc taacttgtgt tacaataagc tagaggtgat ttaatgatta
    32881 aaatattagt tgtagaggat aacattgttc aacaaaaaat cattaccaca aaattaacac
    32941 aagagggcta ccaatttatc actgcttcaa atggacaaga agctctaaat tgtctagata
    33001 cagaagaagt acaattgatt ataactgata tcatgatgcc catgatggat ggttatcaat
    33061 taattcaaga actcaggtca gctgcttata atgttccaat cattgttatg acagcaaaat
    33121 cacaaatgga agatatgaca aaagggtttg gccttggtgc agatgattat atggttaagc
    33181 ctgtgcagtt acaagaatta gctttacgta ttaaggcact cttgaggaga gctaatattg
    33241 tagctcaaca tcagctaatt attgggaata cttgtcttaa tgaagatgaa ttaagcttga
    33301 aatattttga gcaagaaatt atatttcctc agaaagaatt tagggttctt ttccacttac
    33361 ttagctatcc taataggatt ttcacacgtt tagaactatt ggatagtatt tggggaatgg
    33421 atactgattt agatgagcgg gtggtagatg cttgcatcaa taaaattaga cgaaaagtag
    33481 aacacttacc agattttaag attgagacgg tgagaggggt agggtatcgt gcaaaaaatg
    33541 actaagctaa ggcgatttcg gtttccgtta agattttatt tcacattaat gtttgtgtta
    33601 acgatgcttt tctcagtcct agcttctctt ttactagttg cagcaatagt ttttactttt
    33661 tttcaaggag tcttaactac tcatgtatta caagttagtg ctctcgctgt tgttttttta
    33721 agccttgtga tagctagtat tagtatgtgg tacggttctt atcatttaac aaaaccaatt
    33781 ttagatatta gccatattgt gtccaatgtt gcagacggtg actttgaagg gcatatttat
    33841 cgaaacagta atcgtcgtaa atcctatgag tattataatg aattggatga gctatctgaa
    33901 agtattaatc agatgattgt ttctttatct catatggatc atatgcgaaa agactttatc
    33961 actaatgttt ctcatgagct aaagactccg attgctgcag tagctaatat tgtagaacta
    34021 ttacaagatc cagaattaga tgaagaaaca caaagtgagt tattaggtct tgttaaaaca
    34081 gagagcctaa ggttaacaag attatgtgat acgatgttgc aaatgtcgcg cgtggataat
    34141 caggagacta ttggggagct atcctctgtt cgtgtagatg aacaaattcg tcaagcaatg
    34201 attagtttaa cagaacgttg gcaagctaaa aggataaatt ttcaactgga ttcaaaacca
    34261 tatactgttt actcaaactc tgacttgctg atgcaagttt ggataaacct tttagataat
    34321 gcaataaaat attccgagga tattgtagac ttaagtgtac gaatggaaga aactaataat
    34381 cattatctga gagttattat ttctgataaa ggtaggggaa tttctcaata tgatgttcag
    34441 catatatttg ataaatttta ccaagcggat cagtcgcata atcaacaagg aaatggatta
    34501 ggactagcaa tagttaaacg tataattgtt ctttgtaagg gacgtattag tgtctccagt
    34561 cagttggaaa ttggaacaga attttgtgta gaac
//
LOCUS       DATRNK010000016        33782 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.016, whole genome shotgun sequence.
ACCESSION   DATRNK010000016 DATRNK010000000
VERSION     DATRNK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33782
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.016"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..254)
                     /locus_tag="VBC52_000765"
     CDS             complement(<1..254)
                     /locus_tag="VBC52_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 family protein"
                     /protein_id="HEO6882365.1"
                     /translation="MKQEKTGRNLNFWKWAFLLLLAINLSFTAVIASRLIQVREPNTG
                     KISTGVQDKVKVGTFTTNKSQLNKTIALYLKQYQTKKMNY"
     gene            complement(229..1068)
                     /locus_tag="VBC52_000766"
     CDS             complement(229..1068)
                     /locus_tag="VBC52_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEO6882366.1"
                     /translation="MNKKKILTGLSFFLVSLLLSFGIFSLIIPKSNPKLTKKDFLTKK
                     VIPLNYVALGDSLTEGVGDTTSQGGFVPLLSESLHNRYSYQVTSVNYGVSGNTSQQIL
                     KRMTTDPQIEKDLEKADLLTLTVGGNDVLAVIRKELSHLSLNSFEKPAEAYKERLKEI
                     LAKARQDNPKLPIYVLGIYNPFYLNFPQLTKMQTVIDNWNKATKEVVDASENVYFVPI
                     NDRLYKGINGKEGITESSNSQASITNDALFTGDHFHPNNIGYQIMSNAVMEKINETRK
                     NWP"
     gene            complement(1061..1897)
                     /locus_tag="VBC52_000767"
     CDS             complement(1061..1897)
                     /locus_tag="VBC52_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6882367.1"
                     /translation="MSKIKIVTDSSITIEPELIKELDITVVPLSVMIDGTLYSDNDLK
                     AQGEFLNLMRGSKELPKTSQPPVGVFAEIYEKLMNEGVEHIIAIHLTHTLSGTIEASR
                     QGANIAGADVTVIDSTFTDQCQKFQVVEAAKLAKEGADLDTILARVEEVRQKSELFIG
                     VSTLENLVKGGRIGRVTGLLSSLLNIKVIMELTNHELVPIVKGRGLKTFSKWLDNFVE
                     SAQTRKIAEIGISYCGKADMANNFREKLAVLGAPISVLETGSIIQTHTGEDAFAVMVR
                     YE"
     gene            complement(2010..3668)
                     /gene="recN"
                     /locus_tag="VBC52_000768"
     CDS             complement(2010..3668)
                     /gene="recN"
                     /locus_tag="VBC52_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEO6882368.1"
                     /translation="MLLEISIKNFAIIEEISLNFETGMTVLTGETGAGKSIIIDAMNM
                     MLGSRASVEVIRHGANKAEIEGFFSVEKNQSLVQLLEENGIELADELIIRREIFQNGR
                     SVSRINGQMVNLSTLKAVGHYLVDIHGQHDQEELMKPNMHILMLDEFGNTEFNVIKER
                     YQSLFDAYRQLRKRVLDKQKNEQENKSRIEMLEFQIAEIESVALKSDEDQTLLKQRDK
                     LMNHKNIADTLTNAYLMLDNEEFSSLSNVRSAMNDLMALEEFDREYKDLSTNLSEAYY
                     VIEEVTKRLGDVIDDLDFDAGLLQEIENRLDVINTITRKYGGDVNDVLDYFDNITKEY
                     SLLTGSEESSDALEKELKILEHDLIESANQLSLERHKLAKQLENEIKQELTELYMEKA
                     DFQVQFTKGKFNKEGNEIVEFYISTNPGEGFKPLVKVASGGELSRLMLAIKSAFSRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIHKIGSHGQVLAISHLAQVIAIADYQYFIEKIS
                     SDSSTVSTVRLLSYEERVEEIAKMLAGNNVTDTARTQAKELLGS"
     gene            complement(3680..4153)
                     /locus_tag="VBC52_000769"
     CDS             complement(3680..4153)
                     /locus_tag="VBC52_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEO6882369.1"
                     /translation="MKKSERLNLIKQIVLNHAVETQHELLRRLEAYGVTLTQATISRD
                     MNEIGIIKVPSAKGRYIYGLSNENDPIFTTAVAKPIKTSILSISDKLLGLEQFININV
                     IPGNSQLIKTFIMSHCQEHIFSLTADDNSLLLIAKSEADADHIRQSMIAMLEKKD"
     gene            complement(4140..4967)
                     /locus_tag="VBC52_000770"
     CDS             complement(4140..4967)
                     /locus_tag="VBC52_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HEO6882370.1"
                     /translation="MAKERVDVLAYKQGLFDTREQAKRGVMAGMVINVINGERYDKPG
                     EKVADDTELKLKGEKLKYVSRGGLKLEKALQVFEISVADKLTIDIGASTGGFTDVMLQ
                     SGARLVYAVDVGTNQLVWKLRQDHRVRSMEQYNFRYAQKEDFKEGLPEFASIDVSFIS
                     LNLILPALKEILVDGGQVVALIKPQFEAGREQIGKNGIVKDKLVHEKVLTTVTNFTKD
                     YGYTVKHLDFSPIQGGHGNIEFLMHLQKCQDPQNLVLDQIQDVIEKAHKEFKKNEEE"
     gene            complement(4960..5832)
                     /locus_tag="VBC52_000771"
     CDS             complement(4960..5832)
                     /locus_tag="VBC52_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO6882371.1"
                     /translation="MVTIEKIDEAIHRYYKQTHSVVSPDLIKAILYSVDGGGKRIRPR
                     ILLEILEGFGVELIDGHYDVAAALEMIHTGSLIHDDLPAMDNDDFRRGRLTNHKKFDE
                     ATAVLAGDSLFLDPFDLVVKAGFKADVTVRLIELLSMSAGSFGMVGGQMLDMKGENKV
                     LSIDDLSLIHINKTGRLLAYPFVAAGILAEKSEEVKGKLHQAGLLIGHAFQVRDDILD
                     VTASFEELGKTPNKDIVAEKTTYPNLLGLDKSQEILDDTLKKAQAIFQNLEKKANFNA
                     RKIIDIIEGLRLNG"
     gene            complement(5832..6047)
                     /locus_tag="VBC52_000772"
     CDS             complement(5832..6047)
                     /locus_tag="VBC52_000772"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEO6882372.1"
                     /translation="MSDKKTFEENLQELETIVSRLETGDVALEDAIAEFQKGMLISKE
                     LQRTLKEAEETLVKVMQADGTEVEMDT"
     gene            complement(6025..7365)
                     /locus_tag="VBC52_000773"
     CDS             complement(6025..7365)
                     /locus_tag="VBC52_000773"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEO6882373.1"
                     /translation="MSDYLSVSTLTKYLKLKFDKDPYLERVYLTGQVSNFRRRPNHQY
                     FSLKDDKSVIQATMWSGHFKKLGFELEEGMKVNVVGRVQLYEPSGSYSIIVEKAEPDG
                     IGALAIQFEQLKKKLSQAGYFDDRHKQLIPQFVRKIGVVTSPSGAVIRDIITIVSRRF
                     PGVEILLFPTKVQGEGAAQEIAQTIALANEKKDLDLLIVGRGGGSIEDLWAFNEECVV
                     EAIFESRLPVISSVGHETDTTLADFVADRRAATPTAAAELATPVTKIDILSWITEREN
                     RMYQSSLRLIRTKEERLQKSKQSVIFRQPERLYDGFLQKLDNLNQQLTYSMRDKLQTV
                     RQKQGLLHQKLQGIDLKQRIHIYQERVVQSRRLLSSTMTSQYDSKLARFEKAQDALIS
                     LDSSRIVARGYAIIEKNHTLVSTTNGINEGDHLQVKMQDGLLEVEVKDVRQENI"
     gene            complement(7491..8327)
                     /locus_tag="VBC52_000774"
     CDS             complement(7491..8327)
                     /locus_tag="VBC52_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEO6882374.1"
                     /translation="MIVGEQEARALIKPRPKSSHKGDYGSVLLIGGFYPYGGAIIMAA
                     LACVKTGAGLVTVATQSCNIPSLHSQLPEVMAFDSDDYKWLEKSIVQSDVIVIGPGLG
                     VSESSRKILNQTMEKIQSHQSVILDGSALTLLSEGAFPQTKAKNLVLTPHQKEWERLS
                     GIAVSQQTKENTQTALKSFPKGTILVAKSSHTRIFQDLDEKEIIVGGPYQATGGMGDT
                     LCGMIAGMLAQFKEASPLDKVSVGVYLHSAIAQGLSKEAYVVLPTTISDEIPKEMARL
                     SK"
     gene            complement(8324..9178)
                     /locus_tag="VBC52_000775"
     CDS             complement(8324..9178)
                     /locus_tag="VBC52_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEO6882375.1"
                     /translation="MTELIDGKALSQKMQAELGRKVERLKEQHGIIPGLAVILVGDNP
                     ASQVYVRNKERSALEAGFKSETLRLSESISQEELIDIIHQYNEDKSIHGILVQLPLPQ
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPMMVPCTPAGIMEMFREYHVDLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRNLSEVTKEADILIVAIGQGHFVTKD
                     FVKEGAVVIDVGMNRDENGKLIGDVVFEQVAEVASMITPVPGGVGPMTITMLLEQTYQ
                     AALRSVSL"
     gene            complement(9317..11011)
                     /locus_tag="VBC52_000776"
     CDS             complement(9317..11011)
                     /locus_tag="VBC52_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO6882376.1"
                     /translation="MNYKEIYQEWLENDSLGKDIKSDLEAIKGDESEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAIARGIAVSYDVRYQSKEFA
                     ELTCSIMAANGIKSYIYKGIRPTPMCSYAIRALGCVSGVMITASHNPQAYNGYKAYWK
                     EGSQILDDIADQIANHMDAITDYQQIKQIPFEEALASGSASYIDESIEEAYKKEVLGL
                     TINDTNIDKSVRVVYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMPDPDFTTVGYPN
                     PEVPKAFAYSESLGKSVDADILLATDPDCDRVALEVKDSKGEYIFLNGNKIGALLSYY
                     IFSQRCALGNLPHHPVLVKSIVTGDLSKVIADKYNIETVETLTGFKNICGKANEYDIS
                     KDKTYLFGYEESIGFCYGTFVRDKDAVSASMMVVEMTAYYKERGQTLLDVLQTIYDKF
                     GYYNERQFSLELEGAEGQERISRIMEDFRQDPILQVGEMTLENSIDFKDGYKDFPKQN
                     CLKYYFNEGSWYALRPSGTEPKIKCYLYTIGCTEADSLSKLNAIESACRAKMNSTK"
     gene            complement(11298..12032)
                     /locus_tag="VBC52_000777"
     CDS             complement(11298..12032)
                     /locus_tag="VBC52_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6882377.1"
                     /translation="MVEPIISIQGLHKSFGKNEVLKGIDLDIHQGEVVVIIGPSGSGK
                     STFLRTMNLLEVPTKGTVTFEGIDITDKKNDIFKMREKMGMVFQQFNLFPNMTVLENI
                     TLSPIKTKGLSKLDAQTKAYELLEKVGLKEKANTYPASLSGGQQQRIAIARGLAMNPD
                     VLLFDEPTSALDPEMVGEVLTVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMDAGII
                     VEQGAPKEVFEQTKEIRTRDFLSKVL"
     gene            complement(12025..12717)
                     /locus_tag="VBC52_000778"
     CDS             complement(12025..12717)
                     /locus_tag="VBC52_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6882378.1"
                     /translation="MNFSFLPQYWSYFNYGVMVTIMISTCVVFFGTIIGVLIALVKRT
                     NLHFLTILANFYVWVFRGTPMVVQIMIAFAWMHFNNLPTISFGVLDLDFTRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPSGQIEAAYSLGIRPKNTLRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPVAPLLFAAFYYLMLTTILSALLKQM
                     EKYLGKGVKIDG"
     gene            complement(12859..13089)
                     /locus_tag="VBC52_000779"
     CDS             complement(12859..13089)
                     /locus_tag="VBC52_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HEO6882379.1"
                     /translation="MESHLVRIINRLELMATDGGNLKRNFEREGVVVAEVSFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            13387..15648
                     /locus_tag="VBC52_000780"
     CDS             13387..15648
                     /locus_tag="VBC52_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6882380.1"
                     /translation="MLCQNCKLNESTIHLYTNVNGKQKQVDLCQNCYQIIKTDPNNPL
                     FSGLNHVSHAPGGINPFFDDFFGDLNNFRAFNGQDLPNTPPTQSGGNRGGGNGNGRNN
                     NRNQTATPSQAKGILEEFGINVTEIARHGDIDPVIGRDSEIIRVIEILNRRTKNNPVL
                     IGEPGVGKTAVVEGLAQKIVDGNVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKL
                     MEEIRQRQDVILFIDEIHEIVGAGTAGEGSMDAGNILKPALARGELQLVGATTLNEYR
                     IIEKDAALERRMQPVKVDEPSVEETITILKGIQKKYEDYHHVKYNNDAIEAAAVLSNR
                     YIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDQRLIEAENLKAQATREEDYERAA
                     YFRDQIAKYKEMQQQKVDDQDTPIITEKTIEHIIEEKTNIPVGDLKEKEQSQLINLAD
                     DLKQHVIGQDDAVVKIAKAIRRNRVGLGSPNRPIGSFLFVGPTGVGKTELSKQLAIEL
                     FGSADSMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEKVRRNPYSLILLDEIE
                     KAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGSGKTEASVGFGASREGR
                     TNSVLGQLGNFFSPEFMNRFDGIIEFKALDKENLLNIVDIMLSDVNARLAINGIHLDV
                     TDKVKEKLVDLGYDPKMGARPLRRTIQEHIEDAITDYYLENPSEKELRAIMTSNGNII
                     IKSSKKTEESTKG"
     gene            15834..16289
                     /locus_tag="VBC52_000781"
     CDS             15834..16289
                     /locus_tag="VBC52_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6882381.1"
                     /translation="MTNPTFGEKIDNVNYRSRFGVYAIIPNPTHDKIILVQAPNGAWF
                     LPGGEIEENENHLEALTRELIEELGYSATIGHYYGQADEYFYSRHRDTYYYNPAYIYE
                     VTAYHKDQAPLEDFNHLAWFPIQEAKEKLKRGSHRWGVQAWEKNHHSRK"
     gene            16353..16655
                     /locus_tag="VBC52_000782"
     CDS             16353..16655
                     /locus_tag="VBC52_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEO6882382.1"
                     /translation="MRLINTTSSHPELVRNQLQNTDAKLVEVYSAGNTDVVFTKAPKH
                     YELLISNKYRAIKDEELEAIREFFLKRKIDQSIIIQEQMKSLHTAKLIEISYPTTA"
     gene            complement(16772..19564)
                     /locus_tag="VBC52_000783"
     CDS             complement(16772..19564)
                     /locus_tag="VBC52_000783"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEO6882383.1"
                     /translation="MKLKETLNLGQTAFPMRAGLPNKEPQWQEAWDQADIYKKRQALN
                     EGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFRAPYVPGWDTHGLPIE
                     QVLAKKGVKRKEMDLAEYLEMCRDYALSQVDKQRDDFKRLGVSADWENPYITLTPDYE
                     ADQVRVFGAMADKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTVTASRGLTVGPDMEYVVVAPVGSERKYLLAEVLVDSLAAK
                     FGWENFEIVTHHTGKELNHIVTEHPWDTEVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLDVVVTVDSRGLMMENAGPDFEGQFYDKVTPLVKEKLGDLLLASEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKTNFQPEWGKKRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFAEYGSIVWWQRDAKDLLPAGY
                     THPGSPNGLFEKETDIMDVWFDSGSSWNGVMNARENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAVLSQGFVLDGKGEKMSKSLGNTILPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILKQTSETYRKIRNTLRFLIANTSDFNPKQDAVAYENLGAVDRYMT
                     IKFNQVVDTINKAYAAYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSPERRR
                     MQTVFYDILVKLTKLLTPILPHTAEEIWSYLEHEEEEFVQLAEMPVAQTFSGQEEILE
                     EWSAFMTLRTQAQKALEEARNAKVIGKSLEAHLTIYASQEVKTLLTALNSDIALLMIV
                     SQLTIADEADKPADSVSFEGVAFTVEHAEGEVCERSRRIDPTTKMRSYGVAVCDASAA
                     IIEQYYPEAVAQGFEA"
     gene            complement(19848..20618)
                     /locus_tag="VBC52_000784"
     CDS             complement(19848..20618)
                     /locus_tag="VBC52_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEO6882384.1"
                     /translation="MPLTALEIKDKTFSSKFRGYSEEEVNEFLEIVVDDYEDLIRRNR
                     EQEQYIKDLEEKIAYFNEMKESLSQSVILAQETAERVKISAQDEASNLMGKATFDAQH
                     LIDEAKLKANQILRDATDDAKRVAIETEDLKRQSRVFHQRLLSELEGQLKLANSSAWE
                     ELLKPTAIYLQNSDASFKEVVEKVLDEDDALPVVDDTESFDATRQFSPDEMEELQRRV
                     EESNKQLEESGLLDTNNFQMEEPINLGETQTFKLNIED"
     gene            complement(20628..21416)
                     /locus_tag="VBC52_000785"
     CDS             complement(20628..21416)
                     /locus_tag="VBC52_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEO6882385.1"
                     /translation="MTLDDIYQHFRPEEYAFIHKIDHLAQYVENTYSFITTEFLNPRE
                     FKILESVLERRGSHYYTSGQYFQTEYVKVIIAPEYYQLDMVDFNLSLIEIKYNAKFNH
                     LTHAKIMGTLLNYLGVKRSILGDILVEEGYAQVLVDSQMTNHLIHSVTKIGTASVQLA
                     EVPLSKLLTPKQDIQKLTVIASSLRLDKILATILKISRKQSTKLIEADKVKVNYAIVN
                     RVSEQLVEGDLISVRGYGRFTLSHNLGLTKNQKYKLEVDKMIHN"
     gene            complement(21418..21672)
                     /locus_tag="VBC52_000786"
     CDS             complement(21418..21672)
                     /locus_tag="VBC52_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEO6882386.1"
                     /translation="MILVLLILLKTIRVYSYILLAYALLSWFPGAYDSWIGRLVVQIV
                     DPIIRPLRRFNLQFLGLDFTVIVAFFLLNMLSRFLILLFT"
     gene            complement(21675..22280)
                     /locus_tag="VBC52_000787"
     CDS             complement(21675..22280)
                     /locus_tag="VBC52_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEO6882387.1"
                     /translation="MALKDRFDKIISYFDTDDVSENEVHEVQERTSVQRDSRAATAQE
                     ASQRSHMTNSAEEEMIGSRPRTSTYDPNRQERQRVQRDNAYQQTTPRIQNKDSVRQQR
                     EQVTIALKYPRKYEDAQEIVDLLIVNECVLIDFQYMLDAQARRCLDYIDGASRVLYGS
                     LQKVGSSMFLLTPANVMVDIEEMNIPKTGQETSFDFDMKRR"
     gene            complement(22292..22966)
                     /locus_tag="VBC52_000788"
     CDS             complement(22292..22966)
                     /locus_tag="VBC52_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEO6882388.1"
                     /translation="MMNLQENKTAIFDNVSKLALKAGRAPESVHIVAVTKYVNCQTTE
                     ALIRTGVNHIGENRVDKFLEKYQALKDEKLTWHLIGSLQRRKVKDVINYVDYFHALDS
                     VKLAAEIQKRAQKPIKCFLQVNISREDSKHGFTIEQIDDALNLISRYDKIELIGIMTM
                     APLKATKEEISSIFEETESLRKRLQARNIERMPFTELSMGMSRDYDIAIQNGSTFVRI
                     GTSFFK"
     gene            complement(22972..24252)
                     /gene="ftsZ"
                     /locus_tag="VBC52_000789"
     CDS             complement(22972..24252)
                     /gene="ftsZ"
                     /locus_tag="VBC52_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEO6882389.1"
                     /translation="MVFSFDTASVQGAVIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEVLTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVITRPFGFEGNKRSNFAIEGIQELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERITEAARKAIYSPLLETTIDGAEDVIVNVTGGMDMTLTEA
                     EEASEIVSQAAGKGVNIWLGTSIDMDMKDEIRVTVVATGVRKDKTNQVSGFTTSAPTN
                     QAPSERQSTSNSNFDRRGNFDMTESREMPTQQNQPHAQNQQQSSAFGNWDLRRDNISR
                     PTEGELDSKLSMSTFSENDDMDDELETPPFFKNR"
     gene            complement(24274..25647)
                     /gene="ftsA"
                     /locus_tag="VBC52_000790"
     CDS             complement(24274..25647)
                     /gene="ftsA"
                     /locus_tag="VBC52_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEO6882390.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFIANEMNVIGVSNVPSSGVKD
                     GIIIDIEAAATAIKEAVKQAEEKAGITIDKINVGLPANLLQIEPTQGMIPVPNESKEI
                     KDEDVESVVKSALTKSITPEREVISLIPLEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPTTILHNLRKTVERAGIKVEHVVIAPLALAKSVLNEGEREFGATVIDMGGGQTTVAS
                     MRNQELQYTNIYSEGSDYVTKDISKVLRTTVEIAEALKFNFGQANVEEASTSDTVQVN
                     VVGNEEPVEITESYLSQIISGRIRQILEHVKQDLGRGRLLDLPGGIILVGGGAIMPGV
                     VEVAQQIFGTRVKLHVPNQVGIRNPMFANVISIVDYVGMMSEVDIIAQHAVTGDEMLR
                     HKPVDFDYKEKTNTMSTMPYSEPLTSSMEDSNLEPIRARENAQEPTEPKANIGERIRG
                     IFGSMFD"
     gene            complement(25918..27054)
                     /locus_tag="VBC52_000791"
     CDS             complement(25918..27054)
                     /locus_tag="VBC52_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEO6882391.1"
                     /translation="MPKKKSDTPEKEEVVLTEWQKRNLEFLKKRKEDEEEQKRINEKL
                     RLDKRSKLNISSPEEPQNTTKIKKLHFPKISRPKIEKKQKKEKIVNSLAKTNRIRTAP
                     IFVVAFLVILVSVFLLTPFSKQKTITVSGNQHTPDDILIEKTNIQKNDYFFSLIFKHK
                     AIEQRLAAEDVWVKTAQMTYQFPNKFHIQVQENKIIAYAHTKQGYQPVLETGKKADPV
                     NSSELPKHFLTINLDKEDSIKLLIKDLKALDPDLISEIQVISLADSKTTPDLLLLDMH
                     DGNSIRIPLSKFKERLPFYKQIKKNLKEPSIVDMEVGVYTTTNTIESTPVKAEDTKNK
                     STDKTQTQNGQVAENSQGQTNNSNTNQQGQQIATEQAPNPQNVN"
     gene            complement(27058..28134)
                     /locus_tag="VBC52_000792"
     CDS             complement(27058..28134)
                     /locus_tag="VBC52_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEO6882392.1"
                     /translation="MGKKIVFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     EQINQSGLDITFHSIATGKLRRYFSWQNMLDVFKVGVGVLQSIAIIAKLRPQALFSKG
                     GFVSVPPVVAARLLKVPVFVHESDLSMGLANKIAYKFATIMYTTFEQSKDLIKTKHIG
                     AVTKVMDCKKSFENTDLTSIKEAFDPNLKTLLFIGGSAGAKVFNDFITQTPELEEKYN
                     VINISGDSSLNRLKKNLYRVDYVTDLYQPLMNLADVVVTRGGSNTIFELVAMKKLHLI
                     IPLGREASRGDQLENAAYFEEKGYALQLPESELNINTLEKQINLLISNSESYEKNMSQ
                     SSEIKSQDEFYQLLIDDMAKVTKG"
     gene            complement(28137..29492)
                     /locus_tag="VBC52_000793"
     CDS             complement(28137..29492)
                     /locus_tag="VBC52_000793"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEO6882393.1"
                     /translation="MKTITTFENKKVLVLGLARSGEAAARLLAKLGAIVTVNDGKPFD
                     ENPTAQSLLEEGIKVVCGSHPLELLDEDFCYMIKNPGIPYNNPMVKKALEKQIPVLTE
                     VELAYLVSESQLIGITGSNGKTTTTTMIAEVLNAGGQRGLLAGNIGFPASEVVQAAND
                     KDTLVMELSSFQLMGVKEFRPHIAVITNLMPTHLDYHGSFEDYVAAKWNIQNQMSSSD
                     FLVLNFNQGISKELAKTTKATIVPFSTTEKVDGAYVQDKQLFYKGENIMSVDDIGVPG
                     SHNVENALATIAVAKLAGISNQVIRETLSNFGGVKHRLQSLGKVHGISFYNDSKSTNI
                     LATQKALSGFDNTKVILIAGGLDRGNEFDELIPDITGLKHMVVLGESASRVKRAAQKA
                     GVTYSDALDVRDAVHKAYEVAQQGDVILLSPANASWDMYKNFEVRGDEFIDTFESLRG
                     E"
     gene            complement(29622..29867)
                     /locus_tag="VBC52_000794"
     CDS             complement(29622..29867)
                     /locus_tag="VBC52_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEO6882394.1"
                     /translation="MFYLIVALLIALYYFFMAPKTVRNTLNAIGLVGLVALLLVLAVM
                     SFIKILQLPGELYIGLIMIPLGYTAFKETLNLSEKKK"
     gene            complement(29912..31753)
                     /gene="typA"
                     /locus_tag="VBC52_000795"
     CDS             complement(29912..31753)
                     /gene="typA"
                     /locus_tag="VBC52_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEO6882395.1"
                     /translation="MTNLRTDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELEER
                     AMDSNDIEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPSEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSMSDDPSDQEKTMAPIFDTIIDHIPAPVDNSEEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERKEIQEAKAG
                     DLIAVSGMEDIFVGETVTPTDAIEPLPVLRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVQCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLADDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            complement(31985..32365)
                     /locus_tag="VBC52_000796"
     CDS             complement(31985..32365)
                     /locus_tag="VBC52_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO6882396.1"
                     /translation="MDMSVILIIVILLAFVAWASWNYWRVRRAAKFLDNESFQKEMSR
                     GQLIDIREAGAFHRKHILGARNIPASQFKVALSALRKDKPVLLYDASRGQSIPRIVLL
                     LRKEGFNQLYVLKDGFNYWTGRVK"
     gene            complement(32377..33345)
                     /locus_tag="VBC52_000797"
     CDS             complement(32377..33345)
                     /locus_tag="VBC52_000797"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEO6882397.1"
                     /translation="MSKKLLGIDLGGTTIKFGILTLEGEVQEKWAIETNTLENGRHIV
                     SDIVESLKHRLSLYGLTKDDFLGIGMGSPGAVDRTSKTVTGAFNLNWADTQEVGSVIE
                     KEVGIPFFIDNDANVAALGERWVGAGANNPDVVFVTLGTGVGGGVIADGNLIHGVAGA
                     GGEIGHMIVDPENGFTCTCGNKGCLETVASATGVVRVARQLAEQYEGSSAIKAAIDNG
                     DTVTSKDIFIAAEDGDKFANSVVERVSRYLGLAAANISNILNPDSVVIGGGVSAAGEF
                     LRSRVEKYFVTFAFPQVKKSTKIKIAELGNDAGIIGAASLANQQAS"
     gene            complement(33342..33551)
                     /locus_tag="VBC52_000798"
     CDS             complement(33342..33551)
                     /locus_tag="VBC52_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEO6882398.1"
                     /translation="MKVLFDVQNLLKKFGIYVYIGKRLYDIEVMKIELQRLYDNGLIS
                     RDDYLKAELILRREHRLELEKENKK"
     gene            33706..>33782
                     /locus_tag="VBC52_000799"
     CDS             33706..>33782
                     /locus_tag="VBC52_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001884316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEO6882399.1"
                     /translation="MIMSTIYFISLCMSFILSYYDIKYQ"
BASE COUNT        10522 a         6812 c         5279 g        11169 t
ORIGIN      
        1 ttataattca tcttcttagt ttgatattgt tttaaataaa gtgcaattgt cttattcagt
       61 tgcgacttat tggtcgtaaa agtacctact tttactttat cttgtacccc agtcgaaatt
      121 tttcctgtat taggctcacg tacttgaatt aagcgacttg caatcactgc tgtgaaggaa
      181 aggttaattg ccaacaataa gagaaatgcc catttccaga aatttaaatt acggccagtt
      241 ttttcttgtt tcatttattt tctccataac ggcgttagac atgatttgat agccaatatt
      301 attgggatga aaatggtctc cagtaaagag agcatcatta gtgatacttg cctgactatt
      361 tgatgactct gtaataccct ctttaccatt tattccctta taaaggcggt cattaattgg
      421 gacaaaataa acattttctg aagcatcaac tacttctttt gtagctttat tccaattatc
      481 aataacggtt tgcattttag ttaattgtgg aaagtttagg taaaaaggat tataaatgcc
      541 taaaacataa ataggcaatt taggattatc ttgtcttgct tttgcaagga tttctttcaa
      601 acgttcctta tatgcttctg ctggtttctc aaaggaattt agtgataaat gactgagctc
      661 tttacgaata acagccaaga catcattacc accaacagtt agcgtcaata aatcagcttt
      721 ctctaaatct ttttcgattt gaggatctgt cgtcatacgt tttaaaattt gttgactagt
      781 attcccagac acaccataat taacagaagt cacttggtaa gagtatcgat tatggagtga
      841 ttctgatagc agtggaacaa aaccaccttg agaggttgta tcgcccacac cttcggtcag
      901 agaatctcca agagcaacat agttaagtgg gataactttc tttgttagga agtctttttt
      961 tgttaattta ggatttgatt ttggaatgat caaggaaaaa atcccaaaag ataaaagaag
     1021 acttaccaga aaaaaagaaa gtccggtaag tatttttttc ttattcataa cgaaccataa
     1081 cagcaaatgc atcctcacca gtatgagttt ggatgattga gccagtttct aaaacggata
     1141 taggagcacc tagaacagct agtttttctc taaagttatt agccatatca gctttaccac
     1201 agtaggagat gccaatttca gcaatttttc gtgtttgggc actttcaaca aaattatcta
     1261 accatttgct gaaggtcttt agaccgcgac ctttaacaat cggtactaat tcatggtttg
     1321 ttagctccat aattactttt atattaagca atgaacttag aagacccgtt acacgtccaa
     1381 tacgaccacc ttttactaaa ttttcaaggg tcgatacgcc aataaataat tctgacttct
     1441 ggcgtacttc ttccacacga gccaagatgg tatctaaatc agctccctct ttagctaatt
     1501 tcgcagcttc tacaacctgg aatttttgac actggtctgt aaaagtagaa tcaataactg
     1561 taacatctgc accagcaata ttagctccct ggcgtgatgc ttcaatagtt cctgatagtg
     1621 tatgcgttaa atgaattgca atgatatgtt ccacaccttc attcattaat ttttcataaa
     1681 tttctgcaaa aacacctaca ggtggttgac ttgtttttgg caattcctta cttccacgca
     1741 ttaggttcaa gaattcccct tgtgctttta agtcattgtc tgaatacaag gtaccatcta
     1801 tcataactga caagggaaca actgtaatgt caagctcttt aataagttct ggttcaatgg
     1861 taatggaaga atccgttaca attttgattt tactcatatc ctatcctatt ctatacttta
     1921 atatttcaat acccttatta taacaaaatt taagtgtatc aacattaatt tctcctaaca
     1981 atgttagctt tttaaagacg ttaccaaagt taactaccaa gtaattcttt agcttgagta
     2041 cgtgcagtgt ctgttacatt gtttcctgcc aacattttag cgatttcttc tacacgttct
     2101 tcatagctta aaagccttac cgttgaaact gttgaagagt cactggaaat tttttcaata
     2161 aaatattggt aatccgctat tgcaataacc tgagctaaat gagaaattgc taaaacttgt
     2221 ccatgactac caatcttgtg aattttttga gcgatagcct gggccacgcg accagaaact
     2281 cctgtatcca cctcatcaaa tacaatggaa gttttatctt ccttacgtga aaaagcagat
     2341 ttaatagcta acatcaatcg agataactct cctcctgaag ccacttttac caatggctta
     2401 aagccctctc cgggattcgt tgaaatataa aattcaacga tttcgttccc ttctttatta
     2461 aattttcctt ttgtaaattg aacttggaaa tcagctttct ccatataaag ctctgttaat
     2521 tcttgtttga tttcattttc caattgttta gctagcttat ggcgctctaa acttaattga
     2581 tttgctgatt caatcaaatc gtgttctaaa atctttaatt ctttttctaa agcatctgag
     2641 gactcttcac tacctgtcaa tagactatat tctttagtaa tgttatcaaa atagtctaaa
     2701 acatcattca catcaccgcc atattttcgc gttattgtgt taataacatc tagtctattt
     2761 tcaatttctt gtagtaaacc agcgtcaaaa tctaaatcat cgataacgtc acctaaacgt
     2821 ttagtaactt cttcaataac gtagtaagct tctgaaagat tggtggaaag atctttatat
     2881 tctcgatcaa attcttctaa agccataagg tcattcattg cagaacgaac attcgataaa
     2941 cttgaaaact cttcgttatc taacataaga tatgcatttg tcaaagtatc tgcaatattc
     3001 ttatgattca ttaatttatc acgttgcttg agtagcgttt ggtcttcatc tgatttaagg
     3061 gctacagact caatttctgc tatttgaaat tctagcattt caatacgtga tttattctct
     3121 tgttcatttt tttgcttatc caatacgcgt ttacgaagct gacgataagc atcaaaaaga
     3181 ctttgataac gttcttttat gacattaaat tctgtattac caaattcatc taacatcaga
     3241 atgtgcatgt taggtttcat tagttcctct tggtcatgct gaccatgaat gtctaccaaa
     3301 taatgcccca cagcttttag tgttgacaaa ttcaccattt gcccattaat tcgactaacg
     3361 ctgcgcccat tttgaaagat ttctcggcga ataattaatt catctgctaa ttcaatgcca
     3421 ttttcttcca ataattggac taatgattga tttttttcaa cagagaaaaa tccttcaatt
     3481 tctgctttgt tagcaccatg gcgaatcact tcaacgctag cacgggatcc taacatcata
     3541 ttcatagcat caataatgat agatttccct gcaccagtct caccagttaa aacagtcatt
     3601 cctgtttcaa aattaagtga aatttcttcg ataatagcga aattcttaat agaaatttct
     3661 aaaagcataa ggtcctctac taatcttttt tctccaacat tgcaatcatt gattgacgaa
     3721 tgtgatcagc atctgcttct gattttgcaa tcaaaaggag actattatcg tcagctgtca
     3781 aactaaaaat atgttcttga caatgtgaca ttatgaaggt tttaattaat tgactgttac
     3841 ctggtatgac attaatattg ataaattgct ctaaacctag tagcttatct gatattgata
     3901 aaatacttgt tttaataggc tttgccacag cagttgtaaa gatagggtcg ttttcatttg
     3961 acaaaccgta aatatagcga ccttttgctg atggcacttt tataatgcca atttcattca
     4021 tatcacgtga aatagttgct tgagttagag ttaccccata agcttccaat ctgcgtaaca
     4081 actcatgttg tgtttcaaca gcatggttaa gaacaatttg cttaattaaa tttaaacgtt
     4141 cactcttctt catttttctt aaattcctta tgtgcttttt ctataacatc ttgtatttgg
     4201 tcaagcacaa gattttgtgg atcttgacac ttttgcaaat gcattaaaaa ctcaatattt
     4261 ccatgtccac cttgaatggg cgaaaaatca agatgtttaa ccgtatatcc ataatctttc
     4321 gtgaaattgg tcactgttgt caaaaccttt tcatgaacca acttgtcttt gacaatacca
     4381 tttttaccaa tttgctcacg acctgcttca aattgtggtt taattaatgc cactacttgt
     4441 ccaccatcca ctaaaatttc ttttagagct ggtaaaatca aattaagaga gataaatgag
     4501 acatctatcg atgcaaattc aggcagtccc tccttgaaat cttctttttg ggcataccta
     4561 aaattatatt gttccataga acgaacacga tgatcctgac gtaacttcca aactaattga
     4621 tttgttccta catctactgc gtaaactaaa cgcgctcctg attgtagcat aacatcagta
     4681 aaaccacccg tagaggcgcc aatatctata gttagcttat ctgcaactga aatttcaaaa
     4741 acttgtaaag ctttttctaa tttcaatcca cctctactaa catattttag tttttcacct
     4801 tttagtttta attcagtatc gtctgcaacc ttttcacctg gtttatcata acgttctcca
     4861 ttgataacgt taatcaccat tcctgccata acaccacgtt tcgcttgctc tcgtgtatca
     4921 aaaagtccct gtttataggc aagaacatct accctctctt tagccattca accgtaatcc
     4981 ctctattata tctattattt ttctagcatt aaagttagct tttttctcta gattttgaaa
     5041 aattgcctga gcttttttca aagtatcatc aagtatttcc tgtgacttat ccaaacccaa
     5101 taaatttgga taagttgtct tttctgctac aatgtcttta tttggtgtct tccccaattc
     5161 ttcaaaacta gcagtcacat ctaaaatatc atcacgtact tgaaaagcat gaccgattaa
     5221 aaggccagct tgatgcagtt ttccttttac ttcttccgac ttctcagcta aaatacctgc
     5281 tgcaacaaag ggataagcta acaggcgtcc cgttttatta atatgaatca agcttaaatc
     5341 gtcaatagat aaaactttgt tttctccctt catatctaac atttgtccac caaccatgcc
     5401 aaaactaccc gcagacatag acaataactc tattaaccta acagtaacat cagctttaaa
     5461 accagctttt acaactaaat caaatgggtc taaaaaaagg gaatctccag ctaaaaccgc
     5521 tgtagcttca tcaaactttt tatggtttgt taagcggcca cgacgaaaat catcattatc
     5581 catcgccggt aaatcatcat ggattaaaga gccggtgtga atcatctcca aagcagcagc
     5641 cacatcataa tgaccatcta ttagttcaac tccaaatccc tctaaaattt ctaaaaggat
     5701 gcgtggacga atacgtttcc cacctccatc aactgagtag aggatagcct taatcaaatc
     5761 aggagaaacg acactatgtg tctgtttgta ataacgatga atagcttcat caattttttc
     5821 aatagtaacc atcaagtatc catttctact tccgttccgt cagcttgcat cacttttaca
     5881 agcgtctctt cagcttcttt taaagttctt tgtaactctt ttgaaataag cattcctttt
     5941 tgaaattcag caatggcatc ttctaaggca acatcacctg tttctaaacg tgagacaatt
     6001 gtctctaatt cctgtaaatt ttcttcaaat gttttcttgt ctgacatcct tcacctcaac
     6061 ttctaaaaga ccatcttgca tcttaacttg caaatgatct ccttcattta ttccattagt
     6121 agtagatact aaagtatggt ttttttcgat aatagcgtaa ccacgcgcta caattctaga
     6181 actatccaat gatataagcg catcttgagc cttttcaaaa cgtgctagct tgctgtcata
     6241 ctgactagtc attgtacttg ataatagtcg tctactttgc acaacacgct cttggtaaat
     6301 atgaatacgc tgttttaaat ctatgccttg cagtttttga tgaagcaaac cttgcttttg
     6361 tcttactgtt tgtagtttat cacgcataga atatgttaac tgctgattta ggttatctaa
     6421 tttttgaagg aaaccatcat atagacgctc tggttgccta aaaataactg attgctttga
     6481 tttttgcaac ctctcctctt ttgtcctgat aagacgaagg ctagactgat acattctatt
     6541 ttcacgttca gtaatccaag acaagatatc aattttagta accggtgtag caagctcagc
     6601 tgctgcagtt ggagttgctg ctctgcgatc ggctacaaaa tctgctaaag ttgtatcagt
     6661 ttcatgtccg acactagaaa taacgggaag acgagattca aaaatagctt ctacaacaca
     6721 ttcttcatta aatgcccata agtcctcaat agaacctccc ccacggccaa caattaatag
     6781 atccaaatcc tttttctcgt tagctaaagc aatagtttgt gctatttcct gagctgcgcc
     6841 ctctccttga acttttgtgg ggaaaagtaa tatttccact cctggaaatc gacgagatac
     6901 tattgtaata atatcccgaa taacggctcc acttggacta gtcacaactc ctatttttct
     6961 aacaaattga ggaattaatt gcttatgacg atcatcaaaa taacctgctt gagagagttt
     7021 cttctttagt tgttcaaatt ggatagcaag agctcctata ccatccggtt ctgctttttc
     7081 aacaataata gagtaggagc cgcttggttc ataaagttga acacggccaa ctacattaac
     7141 cttcatccct tcctctaatt caaaacccaa ttttttgaag tgacctgacc acattgtggc
     7201 ctgaatgact gatttatcat cttttaaaga gaaatattga tggtttgggc gacgacgaaa
     7261 attagacact tgcccagtta aataaaccct ctctaaataa gggtctttgt caaattttaa
     7321 ttttaaatac ttagtcagtg tcgacacgga taaatagtct gacatagcac ctcctcatga
     7381 gagttttatt aacttcatta taccaaaaaa acttaaaaaa tacctatttg ctctacaaat
     7441 agctcgataa agcaaaagaa gtgtcatgct aacacctctc tacttatttt ttatttagat
     7501 agtctagcca tttcttttgg aatctcatca ctaatcgttg tcggtagaac aacataggct
     7561 tctttagata atccttgagc aattgccgaa tgtagataaa ctcccactga taccttatct
     7621 agaggagagg cttctttaaa ttgcgctaac atacctgcaa tcataccaca caaagtatcc
     7681 cccatccctc cagtcgcctg gtaaggacct cctactataa tttctttttc gtctaaatct
     7741 tgaaaaatac gcgtatgcga actcttagcg actaaaatcg tccctttggg aaaagattta
     7801 agagcggttt gggtattttc ttttgtctgt tgcgatacag cgatacctga caatcgctcc
     7861 cattcttttt gatgaggtgt caacactaaa tttttagcct ttgtttgcgg aaacgcacct
     7921 tctgataaca gagtcaaggc tgatccgtca aggatgacac tttgatgtga ttgaatcttc
     7981 tccatggtct ggttcaaaat ttttcgagat gattctgata ctcctaatcc aggaccaatt
     8041 acaataacat cactttgaac aattgatttt tccaaccatt tgtaatcatc actatcaaac
     8101 gccattacct ctggtagttg actatgcaaa gaggggatat tgcaactttg ggttgctaca
     8161 gtaactaatc ctgcaccagt tttgacacag gccaaagctg ccattataat agcacctcca
     8221 tagggataaa aacctcctat cagaagaaca ctaccataat cacctttatg gcttgattta
     8281 gggcgtggct taattaacgc cctcgcttct tgttcaccaa caatcataga ctcacacttc
     8341 taagagctgc ttgataagtt tgttccaaaa gcattgtaat cgtcataggt cctacgcccc
     8401 caggaacagg tgttatcata ctagcaactt ctgccacttg ttcaaataca acgtctccaa
     8461 tcaatttacc attttcatcg cgattcatac caacatcaat caccacagca ccttctttaa
     8521 cgaagtcttt tgtaacaaag tgcccctgac caatcgcaac aataaggata tcagcctcct
     8581 ttgttacctc agaaaggttt cgagttcttg aatgtgtcaa agtaactgtt gcgtttttat
     8641 caaggagaag ttgtgccatt ggcttaccaa caatattgga ccttccgatg ataacagcat
     8701 gttttccctc taaatccaca tggtactctc taaacatctc cataatacca gctggcgtac
     8761 aaggtaccat catcggtcgt cctgaccaaa gatgacccgt attcattggg tggaaaccat
     8821 caacatcttt cttaggatct attgctaaaa taattttttt atcgttaata tgttgtggta
     8881 acggaagttg aactaaaata ccatggatac ttttatcttc attgtattga tggataatat
     8941 caatcagttc ttcttgagaa atggattcag ataatcgtag agtctcactt ttaaaaccag
     9001 cttctaatgc agagcgttct ttatttcgaa cgtacacttg gctagctgga ttatccccaa
     9061 caaggattac agctaaacca ggtatgatac catgctgttc ctttaacctt tcaacttttc
     9121 gccctaactc agcctgcatt ttttgtgata aggctttacc atctattaat tcagtcattg
     9181 atactccttc ttcatccgtt ttttctatat tatatcaaaa aatagcgatt atgctatttt
     9241 atatcgctat tcattttgta tcaattttaa tcaatagaaa aggcaatgaa tccctcatcg
     9301 cctttttaca tatgacttat ttagtactat tcattttagc acgacaagcc gactcaattg
     9361 cattaagttt cgataaacta tctgcttctg tacaaccaat cgtataaagg taacatttta
     9421 tcttaggttc cgtccctgac ggccttaaag cataccatga accctcatta aaataatatt
     9481 ttaaacaatt ttgctttgga aaatccttat aaccatcctt gaaatcaata gaattctcca
     9541 atgtcatctc acctacttgt aatattgggt cctgtctaaa atcctccata atacgactaa
     9601 tacgttcttg cccctcagca ccctctaact caagagaaaa ttggcgctcg ttgtaatagc
     9661 caaatttatc gtaaatggtt tgcaaaacgt ctaaaagtgt ttgccctcgt tctttataat
     9721 aggcagtcat ttctactacc atcattgaag cactcacagc atctttatca cgtacaaaag
     9781 tgccatagca aaaaccaata ctttcttcat agccaaagag ataagtttta tcctttgaga
     9841 tatcatattc attagctttt ccacaaatat ttttaaatcc tgttaaagtt tcaacagttt
     9901 caatattata tttatctgca ataacttttg atagatcacc agttacaatg gattttacca
     9961 atacaggatg atgtggcaaa ttccctaagg cacatcgttg tgaaaaaata taataggaaa
    10021 gaagtgcccc tatcttatta ccatttaaga aaatatattc tcccttacta tctttgactt
    10081 ccaatgctac tcggtcacaa tctggatctg tggcaagtaa gatatctgca tcaactgact
    10141 ttcctagaga ttctgaatag gcaaatgctt taggaacttc agggtttgga tagccaaccg
    10201 ttgtaaaatc aggatcgggc atttcctgct caggtaccac ataaacattt tcaaaaccac
    10261 ggcgtcttaa aacttcgcgc acaggtaaat ttcctacgcc atttaaagga gtataaacca
    10321 cccggactga cttatcaata ttagtatcat taatagttaa accaagcact tcttttttat
    10381 atgcttcttc aatactctca tcgatataac ttgccgaacc acttgccaga gcctcttcaa
    10441 agggtatttg cttaatttgc tgataatcgg ttatagcatc catatgattg gcaatttgat
    10501 cagcaatatc atctaaaatc tgagatcctt ctttccaata tgccttataa ccattataag
    10561 cttgaggatt atgactagca gtaatcatca cacccgaaac acatcctaga gcacgaatag
    10621 catatgagca cattggtgtt gggcgaatcc ctttataaat ataagactta ataccatttg
    10681 ctgccataat ggaacaagtt aattctgcaa attccttaga ttgataacgg acatcataac
    10741 taactgcaat tccacgtgca atagcttcag ggccatgatc aataatcgta ttagctaatg
    10801 cttgtgctgc tttccccacc atataagtat tcatacgatt ggttcctgct ccaagtttac
    10861 ctctcaatcc cgccgttcca aattctaatg ttttgtaaaa acgatcctga atttcagatt
    10921 catcgccttt aatagcttct aaatctgact taatatcttt accgagtgag tcgttttcta
    10981 accactcttg ataaatctct ttataattca tatgagacat cctattctaa gaccaaaatg
    11041 gtaaaaatca gctccatgat aagagatttt agttcttcag gagctaactg aatagtatca
    11101 aattaacctt ataaagtcaa cttattttat aaattaacta cacttaatat atttaaagca
    11161 tctaaatttt tttcatcacc ctatcattct tagtctacat tatatataga cttaccaact
    11221 tccaaacaat acagtaacaa tttactcata acaatggaat aataaaagtc taaaacaagt
    11281 tgttttagac aatgaaatta taatacttta cttaagaaat ctcttgtgcg gatttctttt
    11341 gtctgctcaa atacttcctt aggggcccct tgctcaacaa taattcctgc atccataaaa
    11401 atgacacgat ccgctacttc acgtgcaaaa cccatttcat gagtgacaat aaccatcgtc
    11461 ataccagatt tagctaaatc ttgcataaca gtcaagactt cacctaccat ttcaggatca
    11521 agagctgaag taggttcatc aaaaagaagg acatcaggat tcattgcaag acctcttgca
    11581 atagcaattc gttgttgttg tcctccagat aagctagctg gataagtatt agccttctct
    11641 ttgagtccaa ctttttcaag tagctcatat gcttttgtct gagcatcaag cttagaaagc
    11701 ccctttgtct taataggtga taaagtaata ttttctagta cagtcatatt gggaaataga
    11761 ttgaactgtt gaaaaaccat gcccattttt tcgcgcattt taaaaatatc attctttttg
    11821 tctgttatat caatcccttc aaaagtcact gttccctttg ttggtacttc caagagattc
    11881 attgttctta aaaatgttga cttaccagag ccagaagggc caataataac cactacttct
    11941 ccttgatgaa tatccaagtc aatgcctttt aaaacctcat ttttcccaaa acttttatga
    12001 agtccttgta ttgaaataat tggctcaacc atctattttt acccctttcc caagatattt
    12061 ctccatttgt tttaacaaag ctgagagaat cgttgtcaac attaaatagt aaaatgctgc
    12121 aaataataac ggtgcaactg gtgagtaagt agccgttaca actgattgtg ctccgttcca
    12181 taattccatg acaccaatag tttgaaggag agcactatct ttaataattg taataaattc
    12241 attccctaga gcaggtaaaa tatttttaaa agcttggggt aagataacat agcgaagtgt
    12301 atttttaggt cgaatcccca acgagtaagc tgcttctatt tgtccagatg gtacagcctc
    12361 aatccctgca cgtacaattt ccgaaatata ggcaccacta tttaaggaaa tgataatgat
    12421 accaggtaaa agtcgtgtaa aatctaaatc taaaacacca aagctaatcg ttggtaaatt
    12481 gttaaaatgc atccatgcga aagcaatcat aatttgaact accatcggtg tcccacgaaa
    12541 tacccataca tagaaattag ctaatattgt gagaaaatgt aaattagtac gctttactaa
    12601 agcaattaac acacctataa tagttccaaa aaaaacaaca catgttgaaa tcataatggt
    12661 taccatcaca ccataattaa aataggacca atattgtggt aaaaatgaaa aattcattga
    12721 catctcctaa aaaagtaatt gccaatagtc tatcacacaa agaataaaaa tacaagttta
    12781 aataaatatt tttgttttta ttctatttta ctgtatattt agatataaaa aaaagacctt
    12841 caaggtcttt tataattatc agtataataa atcgtaaatt tccattgcga taaggtcaat
    12901 gctgtcaaat gaatatgatt cacgcgcttc tacatcacgt aatgtgaaaa caggaccgtt
    12961 ttcagggtca ttactaaaag atacttctgc tacaacaaca ccttcacgtt caaaattacg
    13021 ttttaaatta ccaccatcag tcgccattaa ttcgaggcga ttgatgattc tcactaaatg
    13081 tgattccatc ttacttactc ctatatattt tctagtcttt tctattttaa cacaaaagtg
    13141 accaaagcaa catgaaaagc aacattttta ctaacttttt aacgatttta tcgtctaaaa
    13201 tccgcaaata acgatttttc tcagtagtca tctatttttc aatttagcac tctaatatat
    13261 tcagtgctaa aaacagaact tttttcttga tttctaggct ttaggtgcta taataaaagc
    13321 ataaagtttg actatatttg actatataca catcaatagt taaatgtcat aagaaagggg
    13381 tagcctatgc tctgtcaaaa ctgcaaacta aacgaatcaa ctattcattt gtatacaaat
    13441 gttaatggaa aacaaaagca agttgatctt tgtcaaaatt gctaccaaat tattaaaact
    13501 gaccctaata atccactttt ttcaggttta aatcatgttt cacatgcgcc tggtggcatc
    13561 aatcctttct ttgatgattt ctttggagat ttaaataatt tcagagcctt taatggccaa
    13621 gatttaccaa acactcctcc tacacaatca ggaggaaacc gaggtggagg aaacggtaat
    13681 ggacgaaata ataatcgcaa ccaaactgca actccttctc aagccaaagg gattctagaa
    13741 gaatttggta tcaatgtcac tgaaatagcc cgccatggtg atattgaccc tgttattgga
    13801 cgtgactcag aaattattcg tgttatcgaa atccttaatc gtcgtacaaa aaataatcct
    13861 gtacttattg gcgaacccgg cgttgggaaa actgccgttg ttgaaggact tgctcagaaa
    13921 attgttgatg gtaatgtgcc tcataaactt caaggcaaac aagttattcg tttggatgtt
    13981 gtgagtctag tacaaggaac gggcattcgt ggtcaatttg aagaacgcat gcaaaagttg
    14041 atggaagaaa ttcgtcaacg tcaagatgtt attctattca tagatgaaat tcacgaaata
    14101 gttggagctg gtacagctgg cgaaggtagt atggatgcag gtaatatttt aaaacctgcc
    14161 cttgcacgtg gagaattaca actagtaggt gctactactc tcaatgaata tcgtattatc
    14221 gaaaaggacg ctgccttaga acgccgtatg caacctgtaa aagtcgatga gccttctgtt
    14281 gaagaaacca ttactatttt gaaaggtatc caaaaaaaat acgaagatta tcatcatgta
    14341 aaatataata atgatgccat agaagcagct gcagtactat ctaatcgtta tatccaggac
    14401 cgctttttac ctgataaagc aatagactta ttagatgaag ctggttctaa aatgaaccta
    14461 acactaaatt ttgttgatcc aaaagaaatt gatcaacgtc tcattgaagc agaaaattta
    14521 aaagcgcaag cgactcgtga agaagattac gaacgtgcag cttacttccg tgaccagatt
    14581 gcaaaatata aagaaatgca acaacaaaag gtcgacgatc aagatacacc tattattacc
    14641 gaaaaaacaa ttgagcacat cattgaagaa aaaacgaata tccctgttgg tgatttaaaa
    14701 gaaaaagaac aatctcaatt aattaatctc gcagatgact tgaaacaaca tgtgatcggc
    14761 caggatgacg ctgtcgttaa gattgcaaaa gctattcgtc gtaatcgagt tggtcttggt
    14821 agcccaaacc gtcctattgg ttccttttta tttgtaggac caaccggcgt tggtaaaact
    14881 gaactttcta aacaactagc aattgagctc tttggttcag ctgatagtat gattcgtttt
    14941 gatatgtcag agtacatgga aaagcatgct gttgctaaat tagtcggagc gcctccagga
    15001 tacgtgggat acgaggaagc tggacaatta actgaaaagg ttcgtcgaaa tccttactcg
    15061 ctcatccttc tagatgaaat tgaaaaagct catcctgatg tcatgcatat gttcttgcag
    15121 gtccttgatg acggtcgatt aacagatgga caaggaagaa ctgttagttt taaagatacc
    15181 attatcatca tgacctcaaa tgctggttct ggtaaaactg aagcaagcgt tggctttggt
    15241 gcctcacgag aaggtagaac gaattccgta ctaggtcaac taggtaactt cttcagccct
    15301 gaatttatga accgctttga cggtattatt gaattcaagg ctttagataa agagaatctc
    15361 ctcaatatcg ttgatattat gttatctgac gttaatgcac gtctcgccat caatggtatt
    15421 catctagatg tcactgataa agtgaaagaa aaattggttg atttaggtta cgatcccaaa
    15481 atgggagcac gtccattacg tcgtaccatt caagaacata ttgaagatgc tatcacagat
    15541 tactatcttg agaatccaag cgaaaaagaa cttcgtgcta ttatgactag caatggaaat
    15601 atcataataa aatcttctaa aaaaactgaa gaaagtacaa aaggttgagt aagaaactca
    15661 accttttttc acactcttct caaaaaataa tagcctctat aaagctgttt ctctcctacc
    15721 tcgtctttaa attttaatac aattattaag gaaatattcc aaaaatttta gtataataaa
    15781 taaaaaataa ttaaagcttt taaaatcata aaacacaatc aggagagaga actatgacta
    15841 atcctacttt cggtgaaaaa atagataatg taaactatag gtcacgattt ggtgtctatg
    15901 ctattattcc aaatccaact catgataaaa ttattttagt acaggcacct aatggtgctt
    15961 ggtttcttcc aggtggggaa attgaagaaa atgaaaatca cctagaagct ttaactcgtg
    16021 agttaattga agaattaggt tattccgcaa caattggcca ttactatggt caagctgatg
    16081 aatattttta ctctagacac cgtgatactt actactataa tcccgcctat atttatgaag
    16141 ttactgccta tcataaggat caagcgccac tagaagattt caaccatcta gcctggtttc
    16201 ctatccaaga agctaaagaa aagctaaaac gaggaagcca tagatggggt gtccaagctt
    16261 gggaaaaaaa tcaccattct aggaaataat tcataccttt cttcctaaat agtgttataa
    16321 taaacctaat aagaatcagg gggaaaatag ttatgagact aattaataca accagtagtc
    16381 atcctgaact agttcgaaat caactccaaa atacagatgc aaaattagtg gaagtctatt
    16441 cagctggcaa caccgatgtc gtttttacaa aagcacctaa acattatgaa ttattaattt
    16501 caaacaaata tcgtgctatc aaagatgaag aattagaagc tattcgtgag ttcttcttaa
    16561 agcgtaaaat agatcaatct attattattc aagagcagat gaaatcactc catactgcca
    16621 agttaattga aatttcatat ccaacaacag cataattaaa agaagcctaa tcaaggcttc
    16681 ttttttgatg actactaaac aaatgtctta ccaatttctt ccattaaaac tggtaagata
    16741 tttgtcaaaa tatagcgtta aataattttt attaagcttc aaaaccttga gctactgctt
    16801 caggataata ttgctcaata attgctgcac tagcatcaca aacagctaca ccatacgaac
    16861 gcatctttgt ggttggatca atacggcgtg agcgttcgca gacttcacct tcagcgtgtt
    16921 caacagtaaa tgcaacacct tcaaatgata ctgagtcagc tggtttgtct gcttcatcag
    16981 caattgttaa ttgtgagaca atcatcaaga gtgcaatatc gctatttaac gctgttaata
    17041 acgttttcac ttcttgacta gcataaattg ttaaatgtgc ttctagagat ttaccaataa
    17101 ctttagcgtt acgtgcttct tcgagagctt tttgagcttg tgttcgtaac gtcatgaatg
    17161 ctgaccactc ttcaagaatt tcttcttgac cagagaatgt ctgtgcaaca ggcatttctg
    17221 ctaattgaac aaactcctcc tcctcatgtt cgaggtatga ccaaatttct tcagcagtat
    17281 gtggtagaat cggagtcaaa agttttgtaa gtttaactaa aatatcatag aaaacagttt
    17341 gcatacgacg tcgttctgga ctgttagccg cttcaatgta aaccacatct tttgcaaaat
    17401 caaggtaaaa tgctgataga tcaactgtta cgaagttaac gacagcttta taaattgcca
    17461 taaaatcata agcagcataa gctttgttga ttgtatccac aacttgatta aatttaatag
    17521 tcatataacg atcaacggca cctaaatttt cataggctac tgcatcttgt ttaggattaa
    17581 aatcggaagt atttgcaatc aagaaacgca atgtattcct gatcttacga taagtttctg
    17641 atgtttgttt taagatatcc attgaaatac gaacatcatt acttgagtca acagaagtta
    17701 cccacagacg caagatttcc gcaccaaatt gtttttcaac atcactagga agaattgtat
    17761 ttcctagaga tttagacatt ttctcacctt taccatcaag tacaaaacct tgagataaga
    17821 ctgctttgta tggagcgtga ccattaacag caactgatgt aattagtgat gagttgaacc
    17881 aaccacggta ttgatcagaa ccttcaagat aaagatctgc tggataggaa aggttttcac
    17941 gagcattcat taccccattc catgatgaac cagaatcaaa ccaaacatcc atgatgtctg
    18001 tttctttctc aaagagacca ttaggtgaac ctggatgagt ataacctgct ggtagaaggt
    18061 ccttagcatc acgttgccac caaacgattg agccgtattc agcaaataaa tctgcaacgt
    18121 gatcagtcac ttcttttgtc atgatagctg taccatcttc tgcataaaaa attggaagtg
    18181 gaacacccca agcacgttga cgtgagatta cccagtcgcc acggtcacgg atcatgttat
    18241 aaagacgttt tttaccccac tcaggttgga aatttgtctt ttcaatttca tcgaggattt
    18301 cttgacggaa tttcgaaaca gaggcaaacc attgtggaac cgcacgccaa atgattggct
    18361 ttttcgtacg ccagtcaaat ggatatgagt ggttgataac ttcgctggct aaaagaagat
    18421 ctcccaattt ttcttttaca agtggtgtca ctttgtcata gaattgacct tcaaaatcag
    18481 gaccagcatt ttccatcatc aaaccacgac tatctacggt aactacaaca tcaagtccat
    18541 tagcaatacc aacgttatag tcgtcttcac caaaaccagg agccgtgtgg acaatacctg
    18601 taccagaatc tgttgtaaca tggtctccaa ggataaccaa ctcttctact tctgtatccc
    18661 atggatgttc tgtaacaatg tgattaagtt ctttaccagt gtgatgagtc acaatttcaa
    18721 agttttccca gccaaactta gcagcgagac tatctacaag aacctctgca agaaggtatt
    18781 tacgctcact acctactggt gcaactacaa catactccat atctggtcct actgttaaac
    18841 cgcgtgaagc tgttacagta aatggtgttg tcgtccaaac aacgatatag gtatctgtat
    18901 caagaattcc cttaccatct ttaactttat tggcatagta gagtgatgtc gaatcaatat
    18961 catgatattc gatttcagcc tcagcaaggg cagactctga tgaccatgac caatacactg
    19021 gtttagcacc acgatagata tatcctttat ctgccatagc accgaaaaca cgtacttgat
    19081 ctgcttcata atctggtgtt agtgtaatat aaggattttc ccaatcggca gaaacgccca
    19141 gacgtttaaa atcatcacgt tgtttgtcaa cttggctgag agcataatca cgacacattt
    19201 caaggtattc agccaagtcc atttctttac gtttaacccc ttttttagct aatacttgct
    19261 caattgggag tccatgagta tcccaaccag gcacataagg agctcgaaaa cctgacattg
    19321 attttgaacg tacaataatg tctttcgaaa ttttatttag tgcatgtcct acatgaatat
    19381 ttccattagc atagggaggt ccgtcatgaa ggtgaaaggc tggttttcct tcattcaatg
    19441 cttgacgttt tttataaatg tcagcttgat cccatgcttc ttgccattga ggttccttat
    19501 ttggaagtcc tgcacgcatt ggaaaagctg tttgtcctaa attcaaggtt tcttttaatt
    19561 tcatactaac tccttatcac atagtaatag ttatcacaat ttttaaaatt gctaaataaa
    19621 aaagctttca tcacataaag gacgaaagct cgtggtacca ccttaattca gagcagaaat
    19681 agctatttct tactccctca acaacccgta aggtgggtta agccttctac cttacttaaa
    19741 taatagttca ggtagaatga ttgaaaatga taatttcaat attagggatt gtagggctct
    19801 caccatctcc tactcgctga taatataata ttaaaacctt gtttttctta atcttcaata
    19861 tttaatttaa aagtttgagt ttcacctaaa ttgatcggtt cttccatttg gaaattatta
    19921 gtatctaata aacctgactc ttcaagttgt ttgttacttt cttcaacacg acgctgtaat
    19981 tcttccattt catctggtga gaattgacga gtagcatcaa atgactcagt gtcatccacc
    20041 actggtaaag catcatcttc atcaagaact ttttcaacaa cttctttgaa agaagcatca
    20101 gaattttgaa gataaatagc tgttggtttc aacaattctt cccaagcaga tgagttagct
    20161 aattttaact gaccttctaa ttcagaaagt aagcgttggt ggaaaacacg tgattgacgt
    20221 ttcaaatctt ctgtttctat agcaacacgc ttagcatcat ctgtcgcatc acgaaggatt
    20281 tgatttgctt ttaatttagc ctcatcaatt aaatgttgag catcaaatgt agctttcccc
    20341 attaggttag atgcttcatc ctgtgctgaa attttcacac gttcagcagt ttcttgagct
    20401 aaaataactg attgacttaa cgattccttc atttcgttga agtaagcgat tttctcttct
    20461 aaatctttaa tgtattgctc ttgctcacga ttacgtctaa tcaagtcctc gtaatcgtca
    20521 acaacaatct ctaaaaattc attaacttct tcttcgctat aaccgcgaaa ttttgatgaa
    20581 aatgttttat ctttaatttc aagtgctgta agtggcatgt tttctcctta gttatgtatc
    20641 attttgtcta cttctaactt atatttttga tttttggtta accctaggtt atggctcaac
    20701 gtaaatcgac catatcctct aacactaatc aaatccccct ctactaattg ttcagaaact
    20761 cgattgacga ttgcataatt gaccttgact ttatctgcct caatcaattt tgtcgactgt
    20821 tttcgtgaaa tctttaaaat cgttgctaaa attttatcaa gacgtaaact agaagcaatg
    20881 acagttagtt tttgaatatc ttgtttggga gttagaagtt tggacaaggg aacttcagct
    20941 aattgtacac tagcagttcc aatttttgta actgaatgaa taaggtgatt tgtcatttga
    21001 ctatcgacca aaacctgggc atagccttct tcaactagga tatcccctaa aattgatcgc
    21061 tttacaccta agtaattcag taaagttccc ataattttag catgggtaag gtgattaaat
    21121 ttagcattat actttatttc aattaggcta aggttaaaat caaccatatc taactgatag
    21181 tattccggtg ctattattac tttaacatat tctgtttgaa aatactgacc agaagtgtaa
    21241 taatgactac ctcgcctctc tagaacactt tcgagtattt taaattccct aggatttaga
    21301 aattcagtgg taataaacga ataggtgttt tcgacatatt gagctaaatg gtctatttta
    21361 tggataaacg catactcttc tggtcgaaaa tgctgataaa tatcatctaa cgtcatctta
    21421 tgtaaaaagc aagattaaaa atctactcaa catatttagt aaaaagaatg ctactattac
    21481 tgtaaaatct agacctaaaa attgtagatt aaatcttctg agtgggcgaa taatcggatc
    21541 aacaatctgt accactagtc taccaatcca tgaatcgtat gctcccggaa accaagataa
    21601 caaggcatag gctagtaaga tataagaata gaccctaatt gtttttaata atattaatag
    21661 tactaatatc atatttatcg tctcttcata tcaaaatcaa aactcgtctc ttgaccagtc
    21721 tttggaatat tcatctcctc aatatcgacc ataacattgg ctggtgttaa tagaaacatt
    21781 gaactcccaa ccttttgtaa tgaaccatat aatactctac ttgcaccatc aatataatcc
    21841 aagcaacgtc gtgcttgcgc atcaagcata tattgaaaat caatcaagac acattcatta
    21901 acgataagta aatcaacaat ttcttgtgca tcctcatact tacggggata cttcagagca
    21961 attgttactt gttctctctg ttgtcgaact gagtctttat tttgaatcct aggagtagtt
    22021 tgctgataag cattgtctct ctgtaccctt tgtctctcct gacgattagg atcataggta
    22081 gaagttctcg gacgactacc aatcatctct tcctctgcgg agttagtcat atgactacgc
    22141 tgagaagctt cctgtgctgt agctgctctg gaatctcttt gcactgaagt tctctcttgt
    22201 acttcgtgta cttcattttc acttacatcg tcagtgtcaa aataagaaat aattttgtca
    22261 aatctatctt ttaatgccat attcccctcc attatttaaa gaatgaagtt cctatcctta
    22321 caaatgttga tccattttga atagcaatat cataatctct actcatgccc atgcttaatt
    22381 ctgtaaacgg cattcgttct atgttcctag cttgaagcct tttcctaaga ctctcagtct
    22441 cttcaaaaat tgatgatatt tcctctttag ttgcttttaa aggagccata gtcatgatgc
    22501 cgataagttc aattttatcg taccgagaaa ttaagttcag tgcatcatct atttgctcaa
    22561 tagtaaaacc atgcttactg tcttctcgtg atatattaac ttgtagaaaa catttaattg
    22621 gtttttgagc acgtttttga atctcagctg ctaacttaac agaatctaaa gcatgaaaat
    22681 aatcaacata attaatgaca tctttaactt ttcgacgttg taaactacca atgagatgcc
    22741 atgtaagctt ttcatctttt aatgcttgat atttttcaag aaatttatca acacgatttt
    22801 caccgatatg attaacacct gttctaataa gcgcttctgt tgtttgacag ttaacatatt
    22861 ttgttacagc tacgatatga actgattcag gagcgcgacc tgcttttagt gctaatttac
    22921 taacattgtc aaaaatcgct gttttatttt cttgtaaatt catcatttta attaacggtt
    22981 tttaaagaat ggaggtgttt caagttcatc atccatatca tcattttctg aaaacgttga
    23041 catagacaac ttgctatcaa gttcaccttc tgtcggacgt gaaatattat cacgacgaag
    23101 gtcccaatta ccaaaagcag aactttgttg ctgattttgt gcatgaggct gattttgttg
    23161 agttggcatt tcgcgagact ctgtcatatc aaagttgcca cgacgatcaa agtttgaatt
    23221 tgaagtactt tgacgttctg aaggtgcttg attagttgga gctgatgttg taaaaccaga
    23281 tacttgatta gttttatctt tacgtacacc tgttgcaaca actgttacgc ggatttcgtc
    23341 tttcatatcc atgtcaattg aagtacctaa ccaaatattg acacctttac ctgctgcttg
    23401 gctaacaatc tctgatgctt cctctgcttc agtaagtgtc atatccatac cgccggtaac
    23461 attgacaata acgtcttctg ctccatcaat cgttgtttca agaagtggtg aataaattgc
    23521 ttttcgagca gcttcagtaa tacgttcctc accagaaccg ataccaattc ccatcagtgc
    23581 attaccttta tttgccataa ctgttttaac atctgcaaag tcaaggttaa ttaaacctgg
    23641 gttagtaatc aaatccgtaa taccttgaac accttgacgt aaaacattat ctgcttcact
    23701 aagagcttca agcaatggtg tcttcttatc tacaatttca agaagattat tatttgaaat
    23761 gataagcagt gtatcaactt gttctcttaa ttcttgaata ccttcgatag cgaagttaga
    23821 acgtttatta ccctcaaagc caaaaggacg tgtgatgacc gctactgtaa gtgctcctaa
    23881 actctttgca atacgtgcaa taactggagc cgcacctgta ccagatccgc cccccatacc
    23941 tgctgtgata aataccatat cagcgccagt aagtgcttct gttaaaactt cttcactttc
    24001 ttcagctgct ttacgaccaa cttcaggttg tcctcctgca ccaagtccgc gagttaattt
    24061 tggaccaagt tgaatgacag tttcagcttt tgagctactt aaggcttgaa tatcagtatt
    24121 tgctgcaata aactcaacgc cagcgacacc ttcgtcaatc atgcggttaa tagcgttacc
    24181 gccaccgcct ccaacaccaa taactttaat aactgcacct tgtactgatg ctgtatcaaa
    24241 tgaaaatacc atttttattc ctcacttatt gttttagtca aacatacttc caaaaattcc
    24301 tctgatgcgc tcaccaatat tagctttagg ctcagtaggc tcttgagcgt tttctcttgc
    24361 tcggattggt tctaaattag aatcttccat agaagaggtt aacggctcag aataaggcat
    24421 tgtcgacatt gtatttgttt tttctttata atcaaagtcg actggtttat gtcttagcat
    24481 ttcgtcgcca gttacagcat gttgtgcgat aatgtcaact tctgacatca ttcctacata
    24541 atcaacgata ctaataacat tagcaaacat tggattgcga atacctactt ggtttggaac
    24601 gtgcaattta actctagttc caaaaatttg ttgagcaacc tcgacaactc ctggcataat
    24661 tgcaccacct ccaacaagaa taataccacc aggtaaatca agtaaacgac ctctacccaa
    24721 gtcttgtttg acatgttcta aaatttgacg aatacgtcct gaaataattt gcgaaagata
    24781 gctttctgta atttcaacag gttcctcgtt accaacaaca ttaacttgaa ctgtatcaga
    24841 agtacttgct tcttcaacat tagcttgtcc aaagttgaat ttcaatgctt cggcgatttc
    24901 tactgttgta cgtagcactt tagaaatatc tttggtaaca taatcgctac cttcagaata
    24961 aatattagta tattgcaatt cttgattacg catagaagcg actgttgttt gtccgcctcc
    25021 catatcaatc acagttgcac caaactcacg ttcaccttcg tttagaactg attttgctaa
    25081 ggctagaggg gcgatcacaa catgctctac ttttatacct gctcgttcaa cagttttacg
    25141 aagattatga aggatagttg ttggtccagt atagataaga ccacgcatct caaggcgaat
    25201 ccccatcatc cctctagggt ctctaatccc ttggaaacca tctacaatga attcaagcgg
    25261 aatcaatgaa ataacttctc tttcaggagt aatgctttta gtcaatgctg atttaacaac
    25321 actttcgaca tcttcatcct taatttcttt tgattcatta ggaacaggaa tcattccttg
    25381 agttggttca atttggagaa gatttgctgg taatccaaca ttgattttgt caatggtgat
    25441 gcctgctttc tcctcagctt gttttaccgc ctctttgatt gcagttgctg ctgcctcaat
    25501 atcaataatt ataccatctt ttacaccgga actagggacg ttgctaaccc caattacatt
    25561 catttcattt gcaataaatt ctgcaactaa aactttaatc gagcttgttc ctatatccaa
    25621 accagtaaaa aagccatttc tagccattca ctcgacctcg ctatctttcc attcttttct
    25681 attctacatc tgccaaaaac agaattattc agaaaatatt ataccataaa aaaagcagaa
    25741 ttgttaccaa attacgtttt tttattaagc aacatcaaat tgaaatattt ctaacttatt
    25801 taaaccattc ggaacttata acaaacttaa attttttcaa gaaatcaatt tttactagca
    25861 atctatgact gacttgatat atataaaaaa gactgataaa tccagtcttt tttattgcta
    25921 attaacattt tgagggttag gtgcctgctc tgttgctatc tgttgtcctt gttgattagt
    25981 atttgagtta tttgtttgtc cttgactatt ttccgcaacc tgaccatttt gtgtttgtgt
    26041 tttatcagtt gatttatttt ttgtatcttc tgctttaaca ggggttgatt caatggtatt
    26101 tgttgttgtg taaactccca cttccatatc aacaatagaa ggttccttaa ggttcttctt
    26161 aatttgtttg taaaaaggaa gtctttcttt aaatttagat aatggtattc taatactatt
    26221 tccatcgtgc atatctaaca gcaggaggtc aggtgtcgtt ttagaatcag ctaaacttat
    26281 cacctgaatc tcacttatta aatcagggtc taaagccttt aaatctttaa ttaatagctt
    26341 aatactatct tccttatcaa ggttaattgt taagaagtgc tttggtagct ctgaactatt
    26401 tacaggatca gccttttttc cagtttccaa gacaggttga tatccttgct ttgtatgtgc
    26461 atatgcaata atcttatttt cttgaacttg aatatgaaac ttattgggaa attgataagt
    26521 catctgagct gtttttaccc atacatcttc tgcagctaaa cgttgttcaa tagctttatg
    26581 tttaaaaatt aaagaaaaga aataatcgtt tttttgaata ttcgttttct ctatcaaaat
    26641 atcatcaggt gtatgctgat ttccactaac tgttattgtt ttttgcttac taaaaggagt
    26701 tagtaggaaa acggaaacta aaatgactag gaatgctact acaaatatag gtgcagttct
    26761 aatgcgatta gttttggcta agctgttgac tattttttct tttttctgtt tcttttcaat
    26821 cttaggtctt gaaatctttg gaaaatgaag cttcttaatt ttagtagtat tttgaggttc
    26881 ttcaggagaa gaaatattta atttacttct tttatctaag cgtaattttt cgttaatacg
    26941 tttttgttct tcttcatctt ctttgcgttt ttttaaaaat tcaaggttac gcttttgcca
    27001 ttccgttaag acaacttctt ctttttctgg ggtatctgat ttcttcttag gcatgtttta
    27061 tccttttgtt acttttgcca tatcatcaat taataattga taaaattcat cttgagattt
    27121 tatttcagat gattgtgaca tgtttttttc gtaactctca ctgttagaaa ttaataaatt
    27181 tatctgtttc tcaagtgtat ttatgtttaa ttcagattct ggtaactgca atgcgtagcc
    27241 cttttcttca aaataagcag cattctctaa ttggtcacct cggctcgctt cacgtccaag
    27301 agggataata agatgaagtt ttttcatagc tactaattca aaaatagtat tagagccacc
    27361 tcgtgtaaca accacatctg ctaaattcat taaaggctga taaaggtctg taacataatc
    27421 cacgcgataa aggttcttct tcaatcgatt gagagaagaa tcgcctgaaa tattgataac
    27481 attatatttt tcttccagct caggtgtttg agtaataaaa tcattgaata cttttgcacc
    27541 tgctgatcct ccaataaata gtaaggtctt taggtttgga tcgaaggctt cttttatact
    27601 agttaagtca gtattttcaa acgacttttt acaatccatg acttttgtta cagcaccaat
    27661 atgcttagtt ttgattaaat ctttagattg ttcaaaagtc gtatacataa tagtcgcaaa
    27721 tttataagca atcttatttg ctaaccccat tgataaatct gattcatgaa caaaaacagg
    27781 aacttttaat aggcgtgcag ctactacagg aggtactgaa acaaatcccc cttttgaaaa
    27841 taaggcttgg ggtctaagtt tagcaataat agcaatcgat tgaagaacgc caacccctac
    27901 tttaaagaca tctaacatat tttgccatga aaaatagcga cgtagtttcc cagtagctat
    27961 agagtgaaat gtgatatcaa gtccagactg attaatttgt tcatgttcta tgccattttt
    28021 atcaccaata taatgtactt cccaaccatc tttgataaat ttggggatta atattaggtt
    28081 tagtgttaca tgacctaccg taccaccacc tgtaaaaaca atttttttcc ccatagttac
    28141 tctcctctaa gactttcgaa agtatcaatg aattcatcac cacggacttc gaaattctta
    28201 tacatgtccc atgatgcatt tgcaggactt agcaagataa catcgccctg ttgtgccacc
    28261 tcataagctt tatgtaccgc atctctaaca tctaaagcat cgctataagt tactcctgct
    28321 ttttgtgcag cacgttttac tcgagatgcc gattccccta aaacaaccat atgtttaagt
    28381 ccagtgatat ctggtatcaa ttcatcaaac tcattaccgc gatcaagacc tcctgcaatt
    28441 aggataactt tagtattatc aaagccagat aatgcttttt gagttgccaa tatattagtt
    28501 gacttgctgt cgttatagaa actaatacca tgaaccttac cgagtgattg caagcggtgt
    28561 ttaacacctc caaaattgct taaagtttct ctaataactt gattactgat accagccagt
    28621 ttagcaaccg caatagttgc tagagcattc tctacgttat ggcttcctgg gacaccaatg
    28681 tcatctactg acataatatt ctccccttta tagaaaagtt gcttgtcttg tacgtaagca
    28741 ccatcaactt tttccgtagt agagaaagga acgattgttg ctttagtagt tttagctaac
    28801 tctttagaaa taccttgatt aaaattaagt accaaaaaat cagatgaaga catttgattt
    28861 tggatattcc attttgcagc aacataatct tcaaaagacc catgataatc taaatgagtt
    28921 ggcattaaat tagtaattac tgcaatatga ggacgaaatt ccttaactcc cattagctga
    28981 aaacttgata attccataac tagagtatct ttatcattcg cagcctgaac aacttcacta
    29041 gcaggaaagc cgatattccc agctaacaaa cctctctgac ctccagcatt taagacttct
    29101 gcaatcatcg ttgtcgttgt cgttttcccg ttagagcctg taatacctat tagctgagat
    29161 tctgaaacta agtatgctaa ttccacttca gtcaaaacag ggatttgttt ttctaatgct
    29221 tttttgacca taggattgtt ataaggtatt cctggatttt taatcatgta acaaaaatcc
    29281 tcatctaaca attctaaagg atgactacca caaaccactt taataccctc ttccaacaaa
    29341 gactgtgctg ttggattttc atcaaatggt ttgccatcat taactgtcac tattgctcct
    29401 aacttagcta acaaacgtgc agcagcttct ccagatcgtg ctaaaccaag gactaaaact
    29461 tttttatttt caaatgttgt tattgttttc attactcgtc ccattctatc attattatcc
    29521 ctattatttt acctttaatc agtggctttt tcaaggtaaa ggatatgtct ctaatttctc
    29581 aaaacaaaag aacttaggag acaaaatcct aagttcataa gctatttctt cttttctgac
    29641 aaattcaatg tttctttaaa tgctgtatat cctagaggaa tcattataag cccaatatac
    29701 aattcacctg gcaattgaag gattttaata aaactcataa ctgccaagac aagtaataag
    29761 gctaccaaac ctactaaacc aatagcgttc aaagtattac gtactgtttt tggtgccata
    29821 aaaaagtaat acaaggcaat caaaagggca acaattaagt aaaacataga actcctttct
    29881 tattaatcat aaataagaaa tactttggat attattcagc tgattttttc tttttattag
    29941 ctttgtcacg tgctgcttta tttaaaattt gtttacgaag acgaatagac tcaggagtta
    30001 cttccatgta ctcatcatct gctaaaaatt caagtgactc ttctaaagta aggatacgag
    30061 gcgtcttgat gactgcagtt tgatctttag ttgctgaacg gacatttgtc atttgtttag
    30121 cagttgtaat attgactccg aggtcattat cacgagaatt ctcaccaaca atcattcctt
    30181 cataaacttc tgtacctgga tttacaaaga tagtcccacg ttcttcaata cgcataattg
    30241 aatatgtagt tgctttacca ttttcaatag aaaccaaggc accacgatga cgaccaccaa
    30301 tttctccttg aacaaccggt agatactggt caaaagtatg attcatgata ccatatccac
    30361 gtgtcattga aagaaactct gttgaataac caatcaaacc acgtgcagga atcaagaaaa
    30421 tcaaacgcgt ttgaccatta ccaaccatct gcatatcaag catatcacct ttacgctctg
    30481 acaaactttg gataatagca ccctgatatt cttctggagt atcaatttga acacgctcaa
    30541 acggctcgca ttgaacacca tcaatttctt tgatgataac ttctggacgt gatacttgaa
    30601 gttcatatcc ctcacgacgc attgtttcaa taaggataga taaatgtaat tctccacgcc
    30661 ctgaaaccgt ccatttatct ggcgaatctg ttgggtcaac acgtaaagaa acgtctgttt
    30721 gtaattctgc taaaagacgt tcttcaacct tacgtgacgt aatccattta ccttcgcgac
    30781 ctgcaaatgg tgaattattc accaagaaag tcatttgaag tgttggctcg tcaatacgta
    30841 aaactggtag tggttcaata gcatctgtcg gagttactgt ctcaccaacg aagatatctt
    30901 ccataccaga aacagcgatt aaatcacccg ctttagcctc ttggatttct ttacgttcaa
    30961 gtccaaagaa accaaaaagt tttgttacgc ggaagttttt agttgtacca tcaagttttg
    31021 aaagagtaac ttgatctcca actttgacag tcccgcggaa aacacgtcca ataccaatac
    31081 gtcctacaaa atcattgtaa tcaagaagag aaacttggaa ttgaagtggt tcttccgagt
    31141 tgtcaactgg ggctggaatg tgatcaatga tagtatcaaa aatcggtgcc attgtttttt
    31201 cttgatctga aggatcatct gacattgaag atgttccatt gatagctgaa gcataaacaa
    31261 cagggaaatc tagttgatca tcatcagcac cgagctcaat aaatagttca agaacttcat
    31321 caacaacctc tgatggacga gctgacggct tatcaatttt atttacaaca acgattggaa
    31381 ttaagttttg ttcaagagct ttcttcaaaa caaaacgtgt ttgtggcatt gttccttcgt
    31441 aggcatcaac gactaaaaca acaccatcaa ccattttcat aatacgctca acttcaccac
    31501 caaagtccgc gtgaccaggt gtgtccataa tattgatacg aacatcgttg tatgctacgg
    31561 ctgtattttt tgcaagaatg gtgataccac gttctttttc gatatcattt gaatccattg
    31621 cacgttcttc aagctcttta cgctcatcaa gagtatgtga ttgttttaat aattcatcaa
    31681 cgagagttgt tttaccgtgg tcaacgtggg caatgatcgc aacgttacgg atatctgttc
    31741 ttaaatttgt catgttttcc tctattttat aatgtaattc tgttaactta tgaattataa
    31801 cacaattttt cttaaaaaac agaatttaca caagtgtaaa tgttttcata ttaactgcct
    31861 atttgtgata agaaaacttt tcatcatttc attatttatg ccatttattt tctacttttt
    31921 tcaatttaat tcatttctac aaaaagtgaa gagagttatt aatagtaact ctctccttag
    31981 tatattactt aactctaccc gtccagtaat tgaaaccgtc ctttaacacg taaagttgat
    32041 taaaaccttc ttttctgagt aacaaaacaa tccttggaat agattgacca cggcttgcgt
    32101 catataagag gacaggttta tctttgcgca aagccgataa ggcaacttta aattgacttg
    32161 ctggaatgtt acgtgctccg agaatatgct ttctatgaaa agcaccagct tctcggatat
    32221 caattaactg tcctcgtgac atttcttttt gaaaagactc gttatctaaa aatttagctg
    32281 ccctacgaac cctccaataa ttccaagatg cccaagcaac aaaagccaga agaataacaa
    32341 taattaaaat aacagacata tccatacctt atctccttaa cttgcttgtt gattggctaa
    32401 gcttgctgca ccaataatac cagcatcatt acctagttca gcaatcttaa ttttagttga
    32461 ctttttaact tgtgggaaag caaatgtgac aaagtatttc tcaacgcgac tacgtaaaaa
    32521 ttcacctgct gctgagacac cgccaccaat aaccacagaa tcagggttta aaatatttga
    32581 aatattagct gctgccagtc caaggtaacg tgatacacgt tcaacaacag aattagcaaa
    32641 tttatcccca tcttctgctg ctataaaaat atctttactt gtaacagtat caccgttgtc
    32701 aatcgctgct ttaatggcag acgaaccctc atattgttct gcgagttgac gtgctactct
    32761 aacaacacct gtcgctgatg caactgtctc aaggcagcct ttgttaccac atgtgcacgt
    32821 aaatccattt tctggatcaa caatcatatg cccaatttct ccacctgctc ctgcaacacc
    32881 atggatgagg ttaccatctg cgataacacc tccacctact cctgttccga gggttacgaa
    32941 aacaacgtcg ggattattgg caccagcacc tacccagcgt tcaccaagtg ctgcaacatt
    33001 agcatcgtta tcaataaaaa atggaattcc aacttctttt tcaataactg aacctacttc
    33061 ttgagtatca gcccaattta gattaaaagc acctgttact gttttactag ttctatcaac
    33121 agctcctgga gaacccatac cgataccgag aaagtcatct tttgttaatc catagaggct
    33181 caaacgatgt ttgagagatt caacgatatc agaaacgata tgtcttccgt tttctaaagt
    33241 attggtctca attgcccatt tttcttgtac ttctccctca agcgtcaaga taccaaattt
    33301 aatggtcgtt cctccgaggt caattcccaa taatttctta ctcatttttt attttccttc
    33361 tccaattcta gtctgtgctc tcttcttaaa atcagttctg cttttaaata atcatctcta
    33421 cttatcagac cattatcgta gagacgttgc aattcaatct tcataacttc gatatcataa
    33481 agacgtttac caatataaac ataaatacca aattttttca aaagattctg cacatcgaat
    33541 aaaactttca tatatgaatt ttaacagaaa accattcaat tttcaagaaa atccgcaacc
    33601 gcttgctaat tgtcattatg acgttagact gactatctat aatagaaatc gtaatctctt
    33661 ttctaccatt tttcgaatat aaatataaac tataaaaagg attttatgat tatgtctacc
    33721 atctatttta tttctttatg catgagtttt atcttaagtt attatgatat aaaataccaa
    33781 ga
//
LOCUS       DATRNK010000017        32662 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.017, whole genome shotgun sequence.
ACCESSION   DATRNK010000017 DATRNK010000000
VERSION     DATRNK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32662
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.017"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            149..1207
                     /locus_tag="VBC52_000800"
     CDS             149..1207
                     /locus_tag="VBC52_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO6882400.1"
                     /translation="MSDVVEKQTAKSFIMNVLNGLALGTVIVLIPGAILGELMKALLP
                     MWSGFATLIAATAVATSMMGLVIGIMVGLNFKFNPIQSASLGLAVMFAGGAATFLKGA
                     IMLKGTGDIINMGITAALGVLLIQFLSDKTKSFTLIVIPTVTLLLVGGVGHVLLPYVK
                     MITTMIGQGIASLLGLQPVLMSILIAMIFCFLIVSPITTVGIALAISLSGIGSGAANL
                     GICAASFGLCMAGWSVNSKGTALAHVLGSPKISMANVLAKPKIMLPMISSAAILGILG
                     ALFNIQGTPASAGFGISGLIGPINALNLAKGGWSVMNMLLIIIIFVAAPIILNFIFNY
                     LFIKVLKIIDPMDYKLDI"
     gene            1221..2213
                     /locus_tag="VBC52_000801"
     CDS             1221..2213
                     /locus_tag="VBC52_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEO6882401.1"
                     /translation="MKLKVFNVREEEATLAQDWANRNHVELSMSEGPLTLETVNEVEG
                     FDGIANAQIGPLDDAIYPLLKEMGIKQIAQRSAGVDMYNLELAKQHGIIISNVPSYSP
                     ESIAEFTVTIALNLIRKVELIRANVREQNFSWTLPIRGRVLGNMTVAIIGTGRIGLAT
                     AKIFKGFGCRVIGYDIYHNPMADGILEYVNSVEEAVEKADLVSLHMPPTAENTHLFNL
                     DMFKQFKKGAILMNMARGALVETKDLLEALDQGLLEGAGIDTYEFEGPYIPKNCQGQD
                     ISDKDFLRLINHPKVIYTPHAAYYTDEAVKNLVEGALNACVEVVETGTTTTRVN"
     gene            2420..3970
                     /locus_tag="VBC52_000802"
     CDS             2420..3970
                     /locus_tag="VBC52_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6882402.1"
                     /translation="MEKTMDMEQREEVVSYNRAKVWQLVMFAMNNASTNIYLFTFMFV
                     TYFSTGVLGLAAIFVSQIMGYIRIFDGFIDPAIGIMIDKTDTKFGKYRPILIIGNVIT
                     ALSLIFLLALRGVDENIRFPLFVLVLIIHKIGYSMQQTITKAGQTALTNDPKQRPIFN
                     IVDAVMTTSLMTGGQFVVSGFLVPKFGNFTPQFFNVLIFGTILISAILAIVAIIGIWA
                     KDRKEFFGLGENTQKTALKDYWKVLKGNKPLQILSIAAALVKFAIQFFGDSVVMVLLF
                     GILFGNYALSGQFSLLFIVPGVIINILFSTIARKKGLRFSYVRAIQIGMIGLLAFGAV
                     LYVGKPGDLSLTSLNLYTILFIVTNIIARYASQAPASLVLTMGADISDYETSESGRYV
                     SGMIGTIFSLTDSIASSFAPMVVGFVLAGIGFSKSFPTIETPLTPDLKMAAISLLVAI
                     PFIALSIALLLIKFYKLDKEEMVRIQEKIQVMKAATTKERAKDIARNVPLTDMDYVDV
                     TKYPIDKD"
     gene            4037..5062
                     /locus_tag="VBC52_000803"
     CDS             4037..5062
                     /locus_tag="VBC52_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO6882403.1"
                     /translation="MAKIISLGEVLLRLSPPQYHTLMQANHLKCQFGGSELNVLASLA
                     QLGHHVGLVSALPDNDLGKMARQFILSQQISPAAIIKKEGRLGIYYYEQGFSVRTNKV
                     IYDRNYSSFWESTLSDYDFTSIFKGVDWFHVSGITPALTKDLYEVTRFLMTKAKEGGV
                     KVSIDLNFRESLWSSFQEAREQLSPLLGLSDVCFGLEPIYLAGESEDLKDELGLSRPY
                     LDIELLEKITQKIVQEYGLDYIAFTQREMGYTNQYMLKSYLYHNNMLYQTDKTGVEVL
                     DRVGTGDAFAAGLIHALLEKETPQRALEIAMTTFKYKHTIQGDINIMTRDDIAYLIEK
                     ETNDIKR"
     gene            5079..6878
                     /gene="uidA"
                     /locus_tag="VBC52_000804"
     CDS             5079..6878
                     /gene="uidA"
                     /locus_tag="VBC52_000804"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEO6882404.1"
                     /translation="MLYPLLTKTRNTYDLGGIWNFKLGEHNPNELLPSDEVMVIPTSF
                     NDLMVSKEKRDYIGDFWYEKVIEVPKVSEGEEMVLRFGSVTHQAKIYVDGILVGEHKG
                     GFTPFEVLVPECKYNNEKIKVSICANNVLDYTTLPVGNYSEIIQEDGSIKKKVRENFD
                     FFNYAGVHRPLKLMIRPKNHISDITITSRLSDDLQSADLHFLVETNQKVDEVRISVFD
                     EDNKLVGETKDSRLFLSDVHLWEVLNAYLYTARVEIFVDNQLQDVYEENFGLREIEVT
                     NGQFLLNRKPIYFKGFGKHEDTFINGRGLNEAANLMDLNLLKDIGANSFRTSHYPYSE
                     EMMRLADRMGVLVIDEVPAVGLFQNFNASLDLSPKDNGTWSLMQTKAAHEQAIQELVK
                     RDKNHPSVVMWVVANEPASHEAGAHDYFEPLVKLYKDLDPQKRPVTLVNILMATPDRD
                     QVMDLVDVVCLNRYYGWYVDHGDLTNAEVGLRKELLEWQDKFPDKPIIITEYGADTLP
                     GLHSTWNIPYTEEFQCDFYEMSHRVFDGIPNLVGEQVWNFADFETNLMILRVQGNHKG
                     LFSRNRQPKQVVKEFKKRWMTIPHYHNKKNSVK"
     gene            6907..7578
                     /locus_tag="VBC52_000805"
     CDS             6907..7578
                     /locus_tag="VBC52_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEO6882405.1"
                     /translation="MARPLVEQTADRLLHLILEREYPVGAKLPNEYELAEDLDVGRST
                     IREAVRSLATRNILEVRQGSGTYISSKKGVSEDPLGFSLIKDTDRLTSDLFELRLLLE
                     PRIAELVAYRITDDQLQLLEKLVGDIEDAVHAGDPKHLLLDVEFHSMLAKYSGNIAMD
                     SLLPVINQSIHLINANYTNRQMKSDSLEAHREIIKAIREKNPVAAHDAMLMHIMSVRR
                     SALKA"
     gene            7695..8312
                     /locus_tag="VBC52_000806"
     CDS             7695..8312
                     /locus_tag="VBC52_000806"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HEO6882406.1"
                     /translation="MLNQLKNNYFFAVIRGKSSEDALEIAKHAILGGIRNIEVTFSTP
                     EASKVIKQLSDDFKNNKKIIVGAGTVMTTELAKDAIDAGAKFLVSPHFDSDIANLANE
                     NKVYYFPGCATATEIVVARKYKCQIIKLFPGGVVGPDFIKDIHGPIPDVDLMPSGGVS
                     VSNVAEWRKAGAVAVGVGSALSSKVATEGYDSVTKIAKQFVSALD"
     gene            8329..9729
                     /gene="uxaC"
                     /locus_tag="VBC52_000807"
     CDS             8329..9729
                     /gene="uxaC"
                     /locus_tag="VBC52_000807"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEO6882407.1"
                     /translation="MTFNTETFMLKNQAAIQLYEEVKRQPIFDYHCHLDPKDIFEDRI
                     FDNIVDLWLSGDHYKWRLMRANGISEAEITGPASNLEKFKAFARTLERAYGNPVYHWS
                     AMELKNVFGVNEILTESNAEEIYHRLNHFLKEHKISPRRLIADSKVMFIGTTDHPLDT
                     LKWHKKLAADESFKTVVAPTFRPDEAFIEHRHFVDFITKLGDITQKEITDFSTFIAAM
                     EERIAYFAQNGCRASDISFTEIVFEQTDELELNDLFNKVCEGYIPNQSEISKWQTAVF
                     MELCRLYKKYGFVTQVHFGALRNNHSTIFEKLGADVGVDSLGDQVALTVNMNRLLDSL
                     VKKDSLPKMIWYNLNPAYNIAVANTLANFQANELGVRSYLQFGAGWWFADTKLGMISQ
                     MNALAEQGMLANFIGMLTDSRSFLSYQRHDYFRRILCTYLGEWIEEGEVPEDYQALGS
                     MAKDIAYQNAVNYFKN"
     gene            9747..10793
                     /gene="uxuA"
                     /locus_tag="VBC52_000808"
     CDS             9747..10793
                     /gene="uxuA"
                     /locus_tag="VBC52_000808"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEO6882408.1"
                     /translation="MEMSFRWYGEDDPVTLENIGQIPTMKGIVTAIYDVPVGEVWSRE
                     RIQQLKEKVEAAGLKISVIESVPVHEDIKLGRPTRDLLIDNYIQTVKNLAAEGIDTIC
                     YNFMPVFDWTRTDLAYQYPDGSTALIFDETVSKKMDPVNGELSLPGWDASYSKEEMKA
                     IMDAYAEIDEEKLWENLTYFIKRIIPEAEAVGVKMAIHPDDPPYSIFGLPRIITGLEA
                     IERFVKLYDSKSNGITLCVGSYASDPQNDVLEISRRAFELDRVNFVHARNIKLGDGKS
                     FKESAHPSEYGSIDMYEVIKLCHEFGFEGAIRPDHGRMIWGETGRPGYGLYDRALGAT
                     YLSGLYEAVIKGSK"
     gene            10917..11756
                     /locus_tag="VBC52_000809"
     CDS             10917..11756
                     /locus_tag="VBC52_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO6882409.1"
                     /translation="MSKIITFTNKVVVITGAGGVLCGYMAKEFAKAGAKVALLDLNQE
                     AAQTFADEIVEEGGIAKAYKANVLSKENLEEVHQAVLEDLGPTDILVNGAGGNNPKAT
                     TDNEFHELDLPSETKTFFELDEAGISFVFNLNYLGTLLPTQVFAQDMVGREGANIINI
                     SSMNAFTPLTKIPAYSGAKAAISNFTQWLAVHFSKVGIRCNAIAPGFLVTNQNRSLLF
                     TEDGQPTARAEKILNNTPMGRFGEASELIGGLFFLADEKSSSFVNGVVLPIDGGFAAY
                     SGV"
     gene            11909..12721
                     /locus_tag="VBC52_000810"
     CDS             11909..12721
                     /locus_tag="VBC52_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6882410.1"
                     /translation="MVDTNHKIAFCIDSDGCAMDTMTYKHQLFFGPIAADIFGISEQE
                     RTSFLKEWDRVNLYSRTRGVNRFVGLVMGLEFAGVEHIDKLKEWVETTDSLSNNSLEL
                     ELANDFSRDLEKALEWSNEVNRQIKNYEGDSLAFSGVLEGLEQLSQFGNVYVVSSANK
                     EAVWQEWEEQGLLKYVDDIYCQDRGKKEDVIATLIANGYCRDTILMVGDSPGDLAAAE
                     QNGIAFYPVLVGKEVKSWEILREDIGEAFAKGQFEQQRQKESINTFWANLDN"
     gene            12737..14527
                     /locus_tag="VBC52_000811"
     CDS             12737..14527
                     /locus_tag="VBC52_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEO6882411.1"
                     /translation="MTHLVDLTKKPFNLNQEAIEWIEKTINEMTLDEKIGQLFFNMGA
                     SRSEEYLTDVLDRYHIAAVRYNRGSSSEIYDQNLILQTKSKLPMLIAANTEAGGDGAV
                     TDGTKVGDEIKVAATNDPKYAYEMGRIAGMEASAVGCNASFAPIVDLTRNWRNPIIAS
                     RNWGANVDQIISLSKEYMKGIMQYNIVPFAKHFPGDGIDERDHHLSFASNPMSKEEWM
                     STFGRIYGELADAGLPGVMAGHIHLPNVEKEMHPERDLDDMLPASLNKTLLDELLRGE
                     LGYNGAIVTDASHMVGMTASMSRRDLLPTAIEAGCDLFLFFNDPDEDIQWMKEGYEKG
                     ILTEERLHDALRRTLGLKAKLGLHNYEGRRQELFMPKDKAMALINTSESQKIADEVAD
                     KAVTLVKDKQKDIFPVNPERYRHILLVNVEGYKGGFGAMIAGNKQRASDYMKELLEAR
                     GHEVTVWESTEERIMKLPQEERAAAIANVYAQKQPIANLTDRYDLIINLVDVNAGGTT
                     QRIIWPAAKGTPDQPFYVHEIPSIVISVQHAFALADMPQVGTYINAYDGLPSTISAVV
                     AKLAGESEFTGVSPVDAYCGLIDTHLWRQC"
     gene            complement(14585..15670)
                     /locus_tag="VBC52_000812"
     CDS             complement(14585..15670)
                     /locus_tag="VBC52_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO6882412.1"
                     /translation="MSKLNRIRHHLHSVQAELAVFSDPVTVNYLTGFFCDPHERQMFL
                     FVYEDRDPILFVPALEVSRAKQSVPFPVFGYIDSENPWQKIASNLPSFSVSKVLAEFD
                     NLNVTKFQGLQTVFDGHFENLTPYIQNMRLIKSRDEIEKMLVAGEFADKAVQVGFDNI
                     SLNNTETDIIAQIEFEMKKQGINKMSFDTMVLTGNNAANPHGIPGTNKIENNALLLFD
                     LGVETLGYTSDMTRTVAVGKPDQFKKDIYHLCLEAHQAAIDFIKPGVLASEVDAAARN
                     VIEKAGYGQYFNHRLGHGLGMDVHEFPSIMAGNDMEIQEGMCFSVEPGIYIPDKVGVR
                     IEDCGYVTKTGFEVFTKTPKELLYFEG"
     gene            15880..16884
                     /gene="ccpA"
                     /locus_tag="VBC52_000813"
     CDS             15880..16884
                     /gene="ccpA"
                     /locus_tag="VBC52_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEO6882413.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILARGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPIVLAGTVDLEHQLPS
                     VNIDYKAAAVDVIDILAGNHKDIAFVSGPLIDDINGKVRLAGYKEGLKKNGLNFKEGL
                     VFEANYRYAEGFALAQRVINAGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPIAQYTRPNLTSISQPVYDLGAVSMRMLTKIMHKEELEEKEIVLNHGIVKRGTTNN
                     "
     gene            17016..18482
                     /locus_tag="VBC52_000814"
     CDS             17016..18482
                     /locus_tag="VBC52_000814"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEO6882414.1"
                     /translation="MTNELIMQAFEWYLPSDGNHWKKLEDSISDLKKLGVSKIWLPPA
                     FKGTSSDDVGYGVYDLFDLGEFDQNGTIRTKYGRKEEYLKLIKSLKANGIKPFADIVL
                     NHKANGDHKEKFQVIKVNPENRQEALSEPYEIEGWTGFDFPGRQGEYNDFKWHWYHFT
                     GLDYDAKNNETDIFMIVGDNKGWADDDLIDDENGNFDYLMYNDIDFKHPEVIKNLQDW
                     AKWFIETTGIEGFRLDAVKHIDSYFIQTFINDIRTKIKPDLEVFGEYWKSDQTSMKDY
                     LEATQFQFSLVDVTLHMNFFDASHQNRDFDMRTIFDDSLVIDNPEYAVTFVENHDTQS
                     GQALESRVEDWFKPLAYGLILLRQQGTPCLFYGDYYGIQGEFGQPSFKEVIDKMAELR
                     QNYVFGKQVDYFTHSNCIGWTCLGDEEYNSCLAVVLTNGDQGWKHMEVGEIYAGKTFV
                     DYLGNCEQEVVIGDDGWGDFLVESASISAWVPKIEEAN"
     gene            18527..19525
                     /locus_tag="VBC52_000815"
     CDS             18527..19525
                     /locus_tag="VBC52_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6882415.1"
                     /translation="MKVLLYLEAEEYLKKSGIGRAIKHQEKALQIAGIDYTTNPADDF
                     DLVHMNTYGIRSWLLMSKAKKTGKKVIMHGHSTEEDFRNSFIGSNLVSPLFKWYLCRF
                     YQKADAIITPTDYSKQLIKAYGIKKPIFVLSNGIDLSRYQRSEKKEAAFRHYFHLSKD
                     DKVVMGAGLYFMRKGIDQFVEVAAKMPDIRFIWFGETNKWVIPRKVRQIVTKQHPSNV
                     TFAGYIKGDVYEGAMSASDAFFFPSREETEGIVVLEALASHQHVVLRDIPVYHGWVTE
                     DSVELATDVDGFVEKLDKVLSGKSDKIKEGYHVAESRSIERIAHELASVYQKVMEL"
     gene            19527..20861
                     /locus_tag="VBC52_000816"
     CDS             19527..20861
                     /locus_tag="VBC52_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO6882416.1"
                     /translation="MRIGLFTDTYFPQVSGVSTSIRTLKEGLEKEGHEVYIFTTTDRN
                     VKRFEDPTIIRLPSVPFISFTDRRVVYRGLISAYRIAKDYELDIIHTQTEFSLGLLGK
                     LVAKALRIPVVHTYHTQYEDYVGYIAKGKLIKPSMVKYIMRTYLSDLDGVICPSRIVL
                     NLLDGYGVKIPKQVIPTGIPVENYRREDISEETIKNLRTELGLADNDTMLLSLSRVSF
                     EKNIQAILMHLSAVVDENPHVKLVIVGDGPYLSDLKELVHSLELENSVIFTGMVEHSQ
                     VAIYYKACDFFISASTSETQGLTYIESLASGRPIIAQSNPYLDDVISDKMFGTLYKKE
                     SDLADAILDAIAETPKMTQEAYEQKLYEISAENFSKSVYAFYLDFLISQKASVKEKVS
                     LTIGNKDSHSTLRFVRKAVYLPKKVFTFTGRASKKVVKAPKRRISSIRDFLD"
     gene            21318..23261
                     /gene="thrS"
                     /locus_tag="VBC52_000817"
     CDS             21318..23261
                     /gene="thrS"
                     /locus_tag="VBC52_000817"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEO6882417.1"
                     /translation="MIKITFPDGAIREFESGITTFEIAQSISNSLAKKALAGKFNGQL
                     IDTTRAIEEDGSIEIVTPDHEDALGVLRHSAAHLFAQAAKRLFPDLCLGVGPAIQDGF
                     YYDTDNKSGQISNDDLPRVEEEMKKIVKENHPCIREEISKEEALELFKDDPYKVELIS
                     EHAEDGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNAMMQRVYGT
                     AWFDKKDLKAYLKRREEAKERDHRKLGKELDLFMVNPEVGQGLPFWLPNGATIRRELE
                     RYIVDKEIASGYQHVYTPPMASVEFYKTSGHWDHYREDMFPTMDMGDGEEFVLRPMNC
                     PHHIEVYKHHVHSYRELPIRIAELGMMHRYEKSGALTGLQRVREMTLNDAHIFVTPEQ
                     IKDEFLKALNLIAEIYEDFNLTDYRFRLSYRDPEDKHKYYDNDEMWENAQAMLKEAMD
                     DFGLDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPIMIHRGGISTMERFTAILIETYKGAFPTWLAPQQVSVIPISNEAHIDYAWEVARV
                     LKDRGIRAEVDDRNEKMQYKIRAAQTQKIPYQLIVGDKEMEEKAVNVRRYGSKATETK
                     SIEEFVESILADIARKSRPDEVK"
     gene            23483..24187
                     /locus_tag="VBC52_000818"
     CDS             23483..24187
                     /locus_tag="VBC52_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6882418.1"
                     /translation="MRTVLVVQGDDETIELLRSYLEGALYKVVMASDGEEAFSLFQQH
                     QIDLAIIDITLPKIDGYELTRLIRQDSQIPIIMLAAKTTDMDRILGLNIGADDFITKP
                     FNSLEVLARINSQLRRYYEFNSLAKPKNQFIKIGELELDEEHVELTKNGKHIKLTATE
                     FKILHILMSSPGRIYTKTQLYEKINGRYLEGDETTIMVHISNIRDKIEDDSKYPKYIK
                     TLRGVGYKIEKPQYLV"
     gene            24285..25304
                     /locus_tag="VBC52_000819"
     CDS             24285..25304
                     /locus_tag="VBC52_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEO6882419.1"
                     /translation="MAKTALNIPFTVEGIENYYQNIKMILSLADKWSIPKDDLLLFLK
                     GLKAIDDIKVGSPIFKYFWTSLSLQAPLKALEFVLEQAKMPTELSGELSETQYLVAQF
                     SDELAPHDDFWIALSQVIYDSFPGNSLAEDTVLNRKLHQFRYLISSQQAQYVRRYFKD
                     VGMTDRDALVNYLSCLREPDNIAYYESARLHNKRRRNGEIFAFPDDEPVINSKLLISF
                     HTEFIIDDKGNFLNEIDAEVITSNGIINGASFNYAFKNNTRHKELDVDPVKLDPKFRN
                     DMTRGYRSPNLSRRKWFFFKEEDYDCSYFNKKGYYAFGRRSAKQSVDKQVKYLKKAVQ
                     KMRLN"
     gene            25471..26037
                     /locus_tag="VBC52_000820"
     CDS             25471..26037
                     /locus_tag="VBC52_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO6882420.1"
                     /translation="MIKKNKVFLGVLLVLVVILGGGVLFYQSQFQKTTNQALAIAYKD
                     AKVAKKDVIHQKIDKEFENFRGSYEIEFNTKSAEYSYHVDAKTGQILERDMDNNGFSK
                     STSQSSSSSSQKSHKISQEEAKKIAFKDANIEESEVSNLKIKEEIENGKSVYDIDFVD
                     LKNKNEVDYQIDAETGKIIERSRDHMND"
     gene            complement(26086..26736)
                     /locus_tag="VBC52_000821"
     CDS             complement(26086..26736)
                     /locus_tag="VBC52_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6882421.1"
                     /translation="MSSVLTLTNILFLLQGFGLTLYISFISILLSMFFGTLLAIMRNS
                     KNPIWKLIASIYIEFVRNVPNLLWIFIIFLVFQMKSVSAGITSFTIFTSAALAEIIRG
                     GLNGVDKGQTEAGLSQGFTYLQVFIIIIFPQAFRKMLPAIISQFVTVIKDTSLLYSVI
                     AIQEIFGKSQILMGRYFEAGQVFTLYAIITAVYFITNFIISSFSRKLSKRWEQATE"
     gene            complement(26748..27443)
                     /locus_tag="VBC52_000822"
     CDS             complement(26748..27443)
                     /locus_tag="VBC52_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6882422.1"
                     /translation="MTLLNISPFAISRWGAFFNHFDLFFKGFLYTLGISFGALLLALI
                     LGILSGGLSTSKSKVGKLISRIYVEVFQNTPLLVQMVFVYYGLAIISNGHVMISAFFT
                     AVLCVGLYHGAYISEVIRSGIEAVPKGQTEAALAQGFTANQTMQFIILPQAVRTILPP
                     MTNQVVNLIKNTSTVAIISGADIMFVAKAWAYDTTNYIPAFAGAAIFYFVICFPLASW
                     ARKQEELNKKTYS"
     gene            complement(27456..28256)
                     /locus_tag="VBC52_000823"
     CDS             complement(27456..28256)
                     /locus_tag="VBC52_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6882423.1"
                     /translation="MLRRKRLTLYLLSCIFIFLLFYPNSTSANQLSEIKKSGVLKVGV
                     KQDVPNFGYYNAETNQYEGMEIDIAKKIAKSLGVKPVFVPTTAQTREPLMDNGQIDIL
                     IATYTITPERKANYNISKAYYHDEIGFLVRKNSHIKTIKELDGKHIGVAQGATTKGNL
                     EKYAKEHKLKFSYAQLGSFPELAISLYANRIDAFSVDKSILSGYLSPHTTILKEGFNT
                     QEYGIATSKQDKVLIPYVNKLLVSWEKDGSLKHIYQKFKLKPAKVKKE"
     gene            complement(28268..29023)
                     /locus_tag="VBC52_000824"
     CDS             complement(28268..29023)
                     /locus_tag="VBC52_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6882424.1"
                     /translation="MSLISYKNVNKYYGDYHALRQINLEIEPGQVVVLLGPSGSGKST
                     LIRTMNALESIDDGSLVVNGHELANSSSKELVNLRKEVGMVFQHFNLYPHKTVLENIT
                     LAPIKVLKQSKKEAMEIAEKYLKFVNMWERKDSYPSMLSGGQKQRIAIARGLAMHPKL
                     LLFDEPTSALDPETIGDVLSVMQKLANDGMNMVVVTHEMGFAREVADRIIFMADGEIL
                     VDTTDVQDFFDNPTEPRAKQFLSNIINHTSTDL"
     gene            29363..30073
                     /locus_tag="VBC52_000825"
     CDS             29363..30073
                     /locus_tag="VBC52_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6882425.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYETATAFDGREALVQYAEF
                     QPDLIILDLMLPELDGLEVAKEVRKTSHIPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTENIETAVAEESAQNASSDITIGELQILPDAFIAKKRGEEIE
                     LTHREFELLHHLATHIGQVMTREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPGR
                     PEYILTRRGVGYYMKSYE"
     gene            30066..31415
                     /gene="vicK"
                     /locus_tag="VBC52_000826"
     CDS             30066..31415
                     /gene="vicK"
                     /locus_tag="VBC52_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEO6882426.1"
                     /translation="MNNSAANIRSFELALLFLLVFVAVYFVYLAVRDFKMSKNIRLLN
                     WKVRDLIAGNYSDSILIQGDADLVELGESLNDLSDVFRMAHDNLEQEKNRLASILTYM
                     TDGVLATDRSGKIVMINETAQQQFNLAYDEALSMNIVDMLGSGSPYSFQDLVSKTPEV
                     VLNRRDENGEFVTLRIRFALNRRESGFISGLVAVSHDATEQEKEERERRLFVSNVSHE
                     LRTPLTSVKSYLEALDEGALNEEVAPSFIKVSLDETNRMMRMISDLLSLSRIDNEVTH
                     LDVEMTNFTAFMTSILNRFDQIRNQKTVTGKVYEIVRDYPLKSIWVEIDTDKMTQVID
                     NILNNAVKYSPDGGKITVNLRTTKTQMILSISDQGLGIPKKDLPLIFDRFYRVDKARS
                     RQQGGTGLGLSIAKEIVKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVTYEEWEDVED"
     gene            31419..32228
                     /locus_tag="VBC52_000827"
     CDS             31419..32228
                     /locus_tag="VBC52_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6882427.1"
                     /translation="MSEIGFKYSILASGSTGNCFYIETPQKRLLIDAGLTGKKVTSLL
                     AEINRKPEDLDAILVTHEHSDHIKGVGVLARKYHLDIYANEQTWKVMDERNMLGKVDV
                     SQKHVFGRGTTLTFGDLDIESFGVSHDAVDPQFYRMMKDDKSFVMLTDTGYVSDRMAS
                     LIENADGYLIESNHDIEILRSGSYPWTLKQRILSDKGHLSNEDGSETMIRTIGNRTKH
                     IYLGHLSKENNIKELAHMTMENNLMRADFGVGTDFSVHDTSPDSATPLTRI"
     gene            32231..32599
                     /locus_tag="VBC52_000828"
     CDS             32231..32599
                     /locus_tag="VBC52_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEO6882428.1"
                     /translation="MKKIFYISLGFISLGIGIAGIVLPVVPTTPLVLLSAFCFSRSSE
                     KFDIWLRQTKVYKYYAADFVESRSIAPARKKSMIWQIYILMGISIYFAPLMWLKLGLL
                     IGTIVGTYVLFYVVPDKIDE"
BASE COUNT        10586 a         4985 c         6677 g        10414 t
ORIGIN      
        1 caatctgacc tttatgatta tttccacaat aatattagta aaagctatac ttatataatt
       61 ttttccaatt ttccttttgc aaatagctat gatatagtta atatagttag tgaaatcaca
      121 aacaatcatt attagtagga ggcatcttat gtcggatgtt gtcgaaaaac aaacagctaa
      181 atcgtttata atgaacgtat taaatggttt agctttaggg actgttattg tcttgatacc
      241 aggagcaatc cttggagaat taatgaaagc tttactgcca atgtggagcg gatttgcaac
      301 tttaatcgct gcaacagcag ttgcaacaag tatgatgggg cttgttattg gtattatggt
      361 tggtctcaat tttaaattta atcccattca atcagcatca ctaggattag cggttatgtt
      421 tgcaggtgga gctgcaacct tccttaaagg cgctattatg ctaaaaggaa caggcgatat
      481 cattaatatg ggaattactg ctgccttagg tgtccttttg atccaatttt tatctgacaa
      541 gacgaaatca ttcactttga ttgtcattcc aacagtaaca ctcttactag ttggaggagt
      601 aggtcatgtg ttattacctt atgtcaaaat gattaccacc atgattggac aagggattgc
      661 ttcattactt ggtttacaac ctgttttgat gtcaatttta attgcgatga ttttctgttt
      721 cttgatcgta tctccaatca caacagttgg gattgctttg gcaattagtt tgtccggaat
      781 tggttctggt gctgctaatt taggtatctg tgctgctagt tttgggcttt gtatggctgg
      841 gtggtcagtc aattctaagg ggacagcctt agcgcatgtt ttagggtctc ctaagatttc
      901 gatggctaat gtattagcaa aaccgaagat tatgctacct atgatatctt cagcagcaat
      961 actaggaatt ctcggagctc tctttaatat tcaaggaaca ccagctagtg caggttttgg
     1021 tatcagtggt ttgattggac cgattaatgc tcttaattta gcaaaaggtg gatggtcagt
     1081 tatgaatatg ttattgataa ttataatatt tgttgctgca ccaattatat tgaattttat
     1141 ttttaattac ctctttataa aagtacttaa aattattgat ccaatggact ataaattaga
     1201 tatttaagga gatattagtg atgaaactca aagtatttaa tgttagagaa gaagaagcaa
     1261 cattagcaca agattgggca aaccgaaatc atgtggaact ttctatgagt gaaggacctt
     1321 tgacattgga aactgttaat gaggtagaag gttttgatgg gattgctaat gcacagattg
     1381 ggcctttaga tgatgctatt tatcctcttc tcaaagaaat gggaattaag caaatcgctc
     1441 agcgcagtgc tggtgtcgat atgtataatc ttgagttagc taagcaacat ggtattatca
     1501 ttagcaatgt gccaagttac tcaccggagt ctattgcaga atttacagtg acaatcgctc
     1561 ttaaccttat tcgaaaagtt gagcttatta gagctaatgt tcgtgaacag aatttttcat
     1621 ggaccttgcc aattcgagga cgagtcctag gtaacatgac tgtggctata ataggaactg
     1681 gtcgcattgg tctagcgact gctaaaattt ttaaagggtt cggatgtcgt gttattggtt
     1741 atgatattta ccataatcca atggcagatg gtatccttga gtatgtcaat tcggtagaag
     1801 aagctgttga aaaagctgat cttgtgtcct tacatatgcc accaactgct gaaaatacgc
     1861 atctatttaa tttagatatg tttaaacagt ttaaaaaagg tgctatttta atgaatatgg
     1921 cacgtggagc attggtggaa accaaagatc ttcttgaggc tttagatcaa gggcttttgg
     1981 agggagctgg tattgatact tatgaatttg aggggccata tatacctaaa aattgtcaag
     2041 gtcaggacat ttcagacaag gatttcctaa gactgattaa ccatccgaaa gtaatataca
     2101 ctcctcatgc ggcttattat acagatgaag cggtcaaaaa tctagtagag ggtgccttaa
     2161 atgcttgtgt agaagtggtt gaaacgggaa caactactac gagagtcaac taaaagatgc
     2221 ttaggcatct ttttttgtgg actaacaaaa catatgacga ttatgaatct taaagtaaaa
     2281 aaacatctta tgtatttata gcttaaaagt cttttttata ataaaaaaag atatttttta
     2341 taaaaatatt gacaaaacat cagatgaata gtattattta atcataaaag cgtttacaaa
     2401 aaacaaggag ggttctctca tggagaaaac aatggatatg gaacaacgtg aagaagttgt
     2461 atcttataac cgagcgaaag tatggcagtt agttatgttt gccatgaaca atgcttctac
     2521 aaatatatac ttatttacgt ttatgtttgt tacctatttc tcaactggcg ttcttggctt
     2581 ggcagccatt tttgtcagtc aaattatggg ttatatccgt atttttgatg gttttattga
     2641 tccagctatt ggtattatga ttgataaaac tgatacaaaa tttggtaagt atcgtcctat
     2701 tttgattatt gggaacgtta ttacggcttt gtcattgatt tttcttcttg cattgagagg
     2761 tgtcgatgaa aatattcgtt tcccattatt tgtccttgtt ctaattatcc ataaaattgg
     2821 atactcaatg caacagacaa taactaaagc agggcaaaca gcactgacaa atgaccctaa
     2881 gcaacgtcct atttttaata tcgttgatgc agttatgaca acatcattaa tgacaggcgg
     2941 tcagtttgtc gtttcaggtt tcttagtacc aaaatttggt aattttacac ctcaattctt
     3001 caatgtcctt atttttggaa caattcttat ttcagctatt ttagcaatcg ttgcaattat
     3061 tgggatttgg gcaaaagaca gaaaagaatt ctttggttta ggagaaaata cccaaaagac
     3121 tgctctaaaa gattactgga aggtgttgaa aggaaataaa ccgcttcaga tattatccat
     3181 tgctgcagct ttggtaaaat ttgccatcca attctttggc gattccgttg ttatggttct
     3241 attgtttggt attttatttg gtaattatgc tttatcagga caattctcac ttcttttcat
     3301 tgttcctggt gttattatta atattttgtt ctcaacaatt gctcgtaaaa aaggtcttcg
     3361 tttttcatat gtcagagcaa ttcagatcgg tatgattggt ttacttgctt ttggtgcagt
     3421 tctttacgtt ggtaagcctg gtgaccttag tttaacaagc ctaaatcttt atactatttt
     3481 atttatcgtt actaatatta ttgcgagata cgcttcacaa gcacctgcta gtttagtatt
     3541 gactatggga gcagatattt ctgactatga aacttctgaa tccggtcgtt atgtatcagg
     3601 aatgattgga actatcttct ccttaacaga ttcgattgct tcttcatttg ctcctatggt
     3661 agtaggtttt gtgttagcag gaattggatt ttcaaaatcg tttccaacta tcgaaacacc
     3721 actgacccct gatttaaaaa tggcagctat tagtctcctt gtggccattc catttatagc
     3781 tttgtctata gcccttcttt taataaaatt ttacaaacta gataaagaag aaatggttcg
     3841 tattcaagag aagattcaag ttatgaaagc agcaactact aaggaacgtg ctaaagatat
     3901 tgctcgtaat gttcctttga cagatatgga ttatgttgat gtaacaaaat atcctattga
     3961 taaagattaa aaagtttagc tgctcttcaa tagagcagct attttgttaa ataagagcta
     4021 ccattggagg attcttatgg caaaaattat ttcattaggt gaggttcttt tgcgattatc
     4081 tccacctcaa tatcacacac tgatgcaggc taatcaccta aaatgccaat ttggtggttc
     4141 agaacttaat gtcttagcaa gccttgctca actaggtcac catgtaggat tagtctcagc
     4201 tttgcctgat aatgatttgg gtaagatggc aagacaattt atattgtcac agcaaattag
     4261 tccagctgct ataattaaaa aagaaggacg actaggaatt tattattatg aacaaggatt
     4321 ttcggtaaga acaaataagg ttatctacga tcgtaactat tcatcttttt gggaaagtac
     4381 cttgagtgat tatgatttta cttctatttt taagggagtg gattggtttc atgtttctgg
     4441 aattactcca gcactaacca aggacttata tgaggtgact cggttcctta tgacaaaagc
     4501 taaagaagga ggtgtaaaag tatcgattga tctcaatttt cgtgagagtt tatggtcatc
     4561 ttttcaagag gcgcgtgagc aattatcgcc actattagga ttatcagatg tttgctttgg
     4621 cttagagccg atttatttag ctggtgaaag tgaagattta aaagatgagt tgggcttatc
     4681 cagaccttac cttgatattg aattacttga aaagataact caaaagattg ttcaagaata
     4741 tgggttagac tatattgcct ttactcaaag ggaaatggga tatactaatc agtatatgtt
     4801 aaaatcatat ttgtatcata ataatatgtt atatcaaact gataaaactg gcgttgaagt
     4861 attagatcgt gtggggacgg gagatgcctt tgctgcaggt ttaattcatg cgcttctgga
     4921 aaaagaaact cctcaaaggg ccttagaaat tgctatgact acatttaaat acaaacatac
     4981 cattcaagga gatatcaata tcatgacacg agatgatatt gcttacctca ttgaaaaaga
     5041 aacaaatgat attaaacgat agtgtaaaag gagagtgtat gttatatcca ttattgacaa
     5101 aaacaagaaa tacttatgat ttaggcggta tttggaactt taaattagga gaacataatc
     5161 caaatgaatt actaccttca gatgaagtca tggttatccc gacttcattt aatgatttga
     5221 tggtaagtaa agaaaaacgt gattatatag gtgatttttg gtatgaaaaa gtcattgaag
     5281 tacctaaagt atcagagggt gaagagatgg tgctacgttt tggctcagtg acacatcaag
     5341 ctaaaattta tgttgacggt atattggtag gggagcataa gggaggtttt actccttttg
     5401 aagttcttgt tcctgaatgt aagtacaata atgagaaaat taaggtatca atttgtgcta
     5461 ataacgtttt agactatacc acgcttccgg tcggaaatta tagtgaaatc attcaagaag
     5521 atggtagcat taagaagaaa gtacgtgaga attttgattt cttcaattat gcaggtgtgc
     5581 atcgtcctct taagttgatg atacgtccta aaaatcatat ttctgatatt acaattacaa
     5641 gtcgtctatc agatgattta cagtcagcag atttacattt tttagttgag actaatcaaa
     5701 aggttgacga agttagaatt agcgtttttg atgaagataa caaactagtg ggtgaaacga
     5761 aggatagcag attatttctt agtgatgtcc atctttggga agttttaaat gcctatctgt
     5821 acacagcgcg tgttgaaatt tttgttgata atcaactcca agatgtctat gaggaaaatt
     5881 ttggtcttag agaaatagaa gtgacaaatg gtcaattcct attgaatcgc aaacctattt
     5941 attttaaagg atttggtaaa catgaagata cgttcattaa tggcagaggt ttgaatgaag
     6001 ctgctaactt aatggatttg aatcttttga aggatatagg ggcaaactct tttagaacat
     6061 cccactatcc ttattctgaa gaaatgatgc gtttagcaga tcgaatggga gtgttagtga
     6121 tagatgaggt tccagcagta gggttatttc aaaactttaa cgcttcctta gatttgtcac
     6181 caaaagataa tggtacgtgg agtttgatgc aaacaaaagc tgcgcatgaa caagctatcc
     6241 aagaattagt gaagcgtgat aaaaatcatc ctagcgtcgt gatgtgggta gtcgctaacg
     6301 aaccggctag tcatgaagcg ggagcacacg attattttga gccattagta aaactttata
     6361 aagatttaga ccctcaaaaa cgtcctgtca ccttggttaa tattttaatg gcaaccccag
     6421 atagagacca agtgatggac ctggttgatg ttgtctgcct taatcgttac tatggttggt
     6481 acgttgacca cggtgattta acaaatgcag aagtaggtct aagaaaagag ttattagaat
     6541 ggcaagataa atttcctgac aaaccaatta tcataacgga gtatggcgct gatacgttgc
     6601 cgggattaca ctctacttgg aatattccat atacagaaga atttcaatgt gatttttatg
     6661 aaatgagtca tcgtgttttt gatggtattc ctaatttagt tggtgagcaa gtctggaact
     6721 ttgcagactt tgaaactaat ctgatgatac ttcgtgtaca ggggaatcac aaaggtctct
     6781 tttcaaggaa tcgccagcca aaacaagtcg tcaaagaatt taaaaaacgc tggatgacta
     6841 ttcctcatta ccataataaa aaaaatagtg taaaataggg gtagttattt tagaagaggt
     6901 gatgatatgg ctagaccctt agtagaacaa acagcagatc gtctattgca tttaatttta
     6961 gaaagagaat accctgttgg tgctaagctc cccaatgaat atgaattagc ggaagatcta
     7021 gatgttggtc gcagcacaat ccgagaagca gtcagaagtt tagcaacgcg taatatttta
     7081 gaagtcagac agggctctgg aacatatatt agttctaaaa agggtgtttc tgaggatcca
     7141 ttagggttct ctctaataaa ggacacagat aggttgacat cagatctctt tgaactacgc
     7201 cttttattgg aacctcgaat agcggagctg gtagcttata gaattacaga tgatcagtta
     7261 caattactcg aaaagcttgt tggagatatt gaagatgctg ttcacgcagg cgatccgaaa
     7321 catttacttt tagatgtgga atttcattcc atgctagcta aatacagtgg taatattgca
     7381 atggatagcc ttttgccagt catcaaccag tcaattcatc tgataaatgc gaattataca
     7441 aatcgtcaga tgaagtcgga tagtttagaa gcacatagag aaattatcaa agcaatccga
     7501 gaaaaaaatc cagtagctgc acatgatgct atgcttatgc atatcatgag tgttagacgt
     7561 tcagcattga aagcttaata taagaagaaa aatagctatt cttgaatagc tattttttta
     7621 gtattcttct tgacaattca tcagacgact tatataatat agataaagat aacggttaca
     7681 ttttgggagg tattatgtta aatcaattga aaaacaacta tttcttcgct gttattcgag
     7741 gaaaatcatc tgaggatgca ctagaaatcg caaagcatgc tattttaggt ggtattcgga
     7801 atattgaagt aactttttcg actcctgaag caagcaaagt catcaaacaa ttatcagatg
     7861 actttaaaaa taataaaaaa attattgtgg gggccggaac ggttatgaca actgaacttg
     7921 ctaaagatgc tattgatgct ggagctaaat ttttggttag cccacacttt gattcagata
     7981 ttgctaattt agctaatgaa aacaaggttt attatttccc aggctgtgca acggcaacag
     8041 aaatcgtggt agctagaaag tataaatgtc agattataaa gctttttcca ggtggagttg
     8101 tgggcccaga ttttatcaaa gatattcatg gtcctattcc agatgttgat ttaatgccat
     8161 caggtggcgt ctctgtttca aatgtagctg aatggagaaa agcaggagca gttgcggttg
     8221 gtgttggctc agcattatct agtaaagtag ccacagaagg ctatgatagt gttactaaaa
     8281 tagctaaaca gtttgtttct gcacttgatt agtgagtagg agaagaatat gacgtttaat
     8341 actgaaacat ttatgttaaa aaatcaagca gctatacagc tgtatgaaga ggtcaagagg
     8401 caacctattt ttgattatca ttgccactta gatccaaagg acatttttga agatcgtatt
     8461 tttgataata ttgtggattt gtggcttagt ggtgaccact ataaatggcg tcttatgagg
     8521 gctaatggta tttctgaagc agaaattaca ggtccagcat ccaaccttga gaaatttaaa
     8581 gcatttgcta gaacactaga aagagcatac ggtaatcctg tttaccattg gtctgctatg
     8641 gaattgaaaa atgtctttgg tgttaatgag attttgacag aatcaaatgc tgaggaaatt
     8701 tatcatcgtt taaatcattt cttgaaagag cataaaataa gtcctagacg tttaattgca
     8761 gatagcaaag ttatgtttat tggtactaca gaccatcctc ttgatactct caagtggcat
     8821 aaaaagttgg ctgctgatga gagttttaaa acagttgttg ctccgacatt taggccggat
     8881 gaagctttta ttgaacatcg acattttgtt gattttataa ctaaactggg tgacattact
     8941 caaaaagaga ttacagattt ttcaacattt atagcagcaa tggaagaacg cattgcgtat
     9001 tttgctcaaa atggttgtag ggctagcgat atcagtttta cggaaatcgt ctttgaacaa
     9061 actgatgaat tggagttgaa cgatttattt aataaagttt gtgaaggcta tatcccaaat
     9121 caatcagaaa tcagcaaatg gcaaacagct gtttttatgg aactttgccg attatataaa
     9181 aaatatggtt ttgtaacaca ggtgcatttt ggagctttga gaaataatca ttctactatt
     9241 tttgaaaaat tgggagctga cgtgggtgtg gattcacttg gtgaccaagt ggctttaact
     9301 gttaatatga accgtttatt ggattccttg gttaaaaaag atagtctgcc taaaatgatt
     9361 tggtataatc tcaatccagc ttacaatata gcagtagcta acactttggc aaattttcaa
     9421 gctaacgaac ttggagtgag atcttacctt caatttggtg caggttggtg gtttgcagat
     9481 actaaattag ggatgattag tcaaatgaat gccttagcag agcaaggcat gttggcgaat
     9541 tttattggta tgttaacaga ttctagaagt tttctatctt atcaacgtca tgactatttt
     9601 cgacgtattt tatgtaccta cttaggagaa tggattgaag aaggagaagt tccagaagat
     9661 tatcaagctt taggaagtat ggctaaagac attgcttatc aaaatgcagt taattatttt
     9721 aagaactaaa aaggaagagt gaaatcatgg aaatgtcatt taggtggtat ggtgaagacg
     9781 accccgttac tttagaaaat attggtcaaa ttccaacaat gaaagggatt gttacagcga
     9841 tatacgatgt accagtagga gaagtttggt cacgtgagcg cattcaacag ctgaaagaaa
     9901 aggtagaggc agcagggctt aaaatttcgg ttatcgaatc agttcctgtt catgaagata
     9961 tcaaattggg acgtccaaca cgtgacctct tgattgataa ttatattcaa accgtcaaaa
    10021 atttggctgc ggaaggtatt gatactattt gctataattt tatgccagta tttgactgga
    10081 cacgcactga tttagcatat caatatccag atggttctac agctttgata tttgatgaga
    10141 cggtttctaa aaaaatggat ccagtaaacg gtgaattatc tttgccagga tgggatgcca
    10201 gctatagtaa agaagaaatg aaggctatca tggatgccta tgctgaaatt gatgaggaaa
    10261 aactttggga aaatctcact tattttatta aacgtattat tcctgaagcg gaagccgtag
    10321 gcgttaaaat ggccattcat ccggatgacc caccatattc tatttttggt ttacctcgca
    10381 ttattactgg gttagaggct attgagcgtt ttgtgaaatt gtacgatagt aaaagtaatg
    10441 gcatcacttt atgtgtgggt tcttacgctt ctgatcccca gaatgatgtt ttagagattt
    10501 cgcgacgtgc ttttgagtta gatcgtgtaa actttgttca tgctcgcaat attaaattag
    10561 gtgatggaaa gtcatttaaa gaatcggctc atccatcaga atacgggtca atagacatgt
    10621 atgaagttat taagttatgc catgaatttg ggtttgaggg tgctattcga cctgaccacg
    10681 ggcgtatgat ttggggagaa acaggtcgtc caggatatgg cctatacgat agagctttag
    10741 gagccactta cctttctggt ttatacgaag ctgttattaa aggttcaaaa taaggcgatg
    10801 cttccaaatt tctcctgttc caatctcctg caagagaaat ttggaaagtt attatcataa
    10861 tatgattgta cttagctaag agtagaatac ttatagaagt tagtgagagg gataagatgt
    10921 caaaaataat tacatttacc aataaagttg tcgtcataac aggtgcaggc ggtgttctct
    10981 gtggttatat ggctaaagaa tttgcgaaag caggcgctaa agtggcatta ttagatttga
    11041 atcaagaagc tgctcaaaca tttgctgatg aaattgtaga agaaggtggt atcgctaagg
    11101 cttataaagc taacgttttg tctaaagaaa accttgaaga agtacatcaa gccgttttag
    11161 aggatcttgg accaactgat attttagtta atggtgcagg aggcaacaat cctaaagcca
    11221 caactgataa tgaatttcac gaattagact taccttcaga aacaaaaacc ttctttgagt
    11281 tagatgaagc tggtattagc tttgttttta acttaaatta tttaggaaca ttattgccaa
    11341 cgcaagtatt tgcccaagat atggttggac gtgaaggagc gaatattatt aatatctctt
    11401 caatgaatgc tttcacccct ctgaccaaaa taccagctta ttctggagct aaagcagcta
    11461 ttagtaactt cacacaatgg ctagctgttc atttctcaaa agttggtatc cgttgtaatg
    11521 ctattgcacc tggtttcttg gtaacaaatc agaaccgaag cttgttattt actgaggacg
    11581 gtcagccaac tgcgcgtgct gagaaaattc ttaataatac accaatggga agatttggag
    11641 aagcaagtga gctgattggc ggattgttct ttttagcaga tgaaaaatca tcaagttttg
    11701 ttaatggcgt tgtccttccg attgatggtg gatttgctgc ttactctggt gtttaattta
    11761 taagataata gctacttaac atataagaga ttattgagtt gagtggtttt ttaaggaaat
    11821 ttgtttaatg ctcttaccac taaatgatgg gtaggttgct aattggaaaa tcctcataag
    11881 ttagataaat ttagaaactg gagataatat ggtagataca aatcacaaaa tcgctttttg
    11941 catagattcc gatggttgcg caatggatac aatgacatat aagcatcaac tctttttcgg
    12001 tccaattgct gcagatatat ttgggatatc agaacaagaa agaacaagtt ttttaaaaga
    12061 gtgggataga gtcaatcttt attcaagaac tcgtggagtt aatcggtttg tgggattggt
    12121 tatgggcctt gagtttgccg gagtagaaca tattgataaa ttaaaagaat gggtcgaaac
    12181 aacagattca ttgtccaata attccttaga gttagagctt gccaacgatt ttagtagaga
    12241 tttagagaag gctttagaat ggtccaatga agtgaatcgc caaatcaaaa attatgaggg
    12301 agattcttta gcgttttcag gtgtgttaga aggtttagaa caattaagtc aatttggtaa
    12361 tgtttatgtc gtgagttctg ctaacaagga agctgtttgg caagagtggg aagaacaggg
    12421 attgctaaag tatgttgatg atatctattg tcaagataga ggtaaaaaag aagatgttat
    12481 cgccacttta attgcaaatg gttactgccg tgacactatt ttgatggtag gagattcccc
    12541 gggtgatttg gctgcagcag aacaaaatgg tattgcattt tacccagtac ttgttggtaa
    12601 ggaagtcaaa tcatgggaaa ttcttagaga agatattggt gaagcttttg caaaaggtca
    12661 attcgaacag caaaggcaaa aagaatctat taataccttt tgggctaatt tagataacta
    12721 atagaaagga taggctatga cacatttagt tgacttaaca aagaaaccct tcaatctaaa
    12781 tcaagaagca attgagtgga ttgaaaaaac aattaatgag atgacattgg atgagaaaat
    12841 tgggcaactt tttttcaata tgggagctag tcgttcagaa gaatatttaa cagatgtttt
    12901 ggatcggtat catattgctg ctgttcgcta taatagaggt agttcaagtg aaatctatga
    12961 tcagaattta attttacaga caaagtcgaa attgccgatg ttgattgctg caaatacaga
    13021 agctggtgga gacggtgctg tcacggatgg aactaaagtt ggcgatgaaa taaaagtggc
    13081 tgctacaaat gatccgaaat atgcttatga aatgggaaga atcgcaggga tggaggcatc
    13141 tgctgtgggt tgtaatgctt catttgctcc aattgtagat ttgacacgta attggcgtaa
    13201 tcctatcata gctagtcgaa attggggtgc taacgttgat caaattattt cactttcaaa
    13261 agagtatatg aaaggaatca tgcaatataa tattgtacct ttcgctaaac attttccagg
    13321 ggatggcatt gatgagcgtg atcatcattt gtcatttgct tccaatccta tgagtaaaga
    13381 ggaatggatg tcgacatttg gccgcattta tggtgaattg gctgacgcag gtctaccagg
    13441 agtcatggca ggtcacatac atttaccaaa tgttgaaaaa gaaatgcatc cagagcgaga
    13501 ccttgatgat atgttaccag cttcattgaa taaaacactt ttagatgagt tattgagagg
    13561 agagttaggt tataatggag ctattgtaac ggacgcttct catatggttg gaatgactgc
    13621 ttcaatgtca aggagagatt tgttgccaac ggcgattgaa gcaggttgtg acttattctt
    13681 attctttaat gaccccgatg aagacattca atggatgaaa gaaggttacg aaaaaggtat
    13741 cctaacagag gaacgtctcc atgacgcttt acgtcgtact ctcggtctaa aagcgaaatt
    13801 aggcttgcat aactatgagg gacgtcgcca agaactcttt atgcctaaag ataaggctat
    13861 ggctttaatc aacacatcag agtcgcaaaa aatagctgat gaagttgctg ataaagcagt
    13921 aactctggta aaagataaac aaaaagatat ctttccagta aatccagaac gttatcgtca
    13981 tatcttactt gttaacgttg aaggatataa gggaggtttt ggtgctatga ttgctggaaa
    14041 caagcaacgt gcatcggatt acatgaaaga gttacttgag gcacgtggcc atgaagtaac
    14101 tgtctgggag tctaccgaag agcgcattat gaaattgcct caagaggagc gggcagcagc
    14161 tatcgctaat gtttacgcac aaaaacagcc aatcgcaaat ctaacagatc gctatgacct
    14221 gattattaac ttggtagatg tcaatgctgg aggaacaaca caacgtatta tttggccagc
    14281 agctaaaggg acccctgatc agccttttta cgttcatgaa ataccatcta ttgtgatttc
    14341 agtacaacat gcatttgctt tagcagatat gccacaggta gggacttata ttaatgctta
    14401 cgatgggctt cctagcacga taagtgcagt agttgcgaaa ttggctggag aatcagaatt
    14461 tacaggagtt tcaccagtag atgcttactg tggtttaatt gatacacatt tatggcgtca
    14521 atgctaatag aaacaagtta ttgtactttt atgtaacata aaaaaagagc ttaaagctct
    14581 ttttttatcc ttcaaaatag agtagttcct tgggtgtttt ggtaaatact tcaaagccgg
    14641 ttttagtcac gtaaccgcag tcttcaattc gcacaccaac tttatctggt atataaatac
    14701 ctggttcaac agagaagcac atgccttctt gaatctccat atcgttacca gccataattg
    14761 atggaaattc atggacgtcc atacctaatc catgaccaag gcggtggtta aagtattgtc
    14821 cataaccagc cttttcaata acatttcttg ctgctgcgtc aacttcagaa gcaaggactc
    14881 ctggcttaat aaaatcaata gccgcttgat gtgcttcaag acataaatgg taaatatctt
    14941 ttttgaattg atcaggtttc cccactgcta ctgtacgggt catatctgac gtataaccta
    15001 gtgtttctac acctaaatca aataataaca aagcattatt ttcgatttta ttagttcctg
    15061 ggataccatg tggatttgct gcattattgc cagtcaaaac catagtgtcg aaactcattt
    15121 tattgatacc ttgttttttc atttcaaatt cgatttgagc gataatatcg gtttcagtgt
    15181 tgtttagcga gatattgtca aatccaactt gaacagcttt atcagcaaat tctccagcga
    15241 ctaacatttt ctcaatttca tctcgtgatt taattaggcg catgttttga atataaggtg
    15301 ttaggttttc aaaatgacca tcaaaaacgg tttggagacc ttggaattta gtaacattaa
    15361 gattatcaaa ttcggctaga actttagaaa cagaaaagga tggtaagttg ctagctattt
    15421 tttgccaagg attttctgaa tctatgtaac caaagacggg aaatggtaca ctttgttttg
    15481 cacgcgatac ttctagtgct ggtacaaata aaataggatc gcgatcttca tagacaaata
    15541 aaaacatttg tctttcatgg ggatcgcaaa agaaacctgt taaatagttg actgtcacag
    15601 gatcagaaaa gacagcaagt tcagcttgga ctgaatgtaa gtgatggcgt atacgattaa
    15661 gttttgacat aataaccctt cttcctaaaa ggaacttagt ctagttaggc tagtttctta
    15721 tgcctattat ttttccaaaa aaataggaaa aaatcaagtt tagttagtat tttgaaagtt
    15781 tttcataaaa aagctttata aaagcttgaa agtgtttcca aaaggtgata aaatatcagt
    15841 gaaagcgtca ttttcaaata gaaaggaagt taaatcaata tgaatacaga tgatacgatt
    15901 acgatttatg atgttgcccg tgaagcaggt gtttcgatgg caactgttag tcgtgtggta
    15961 aatggaaata aaaatgtcaa ggaaaataca cgtaagaaag tccttgaagt tattgatcgt
    16021 cttgactatc gtccaaatgc tgtggcgcgt ggtttagcaa gtaaaaaaac gacaactgtt
    16081 ggtgttgtca ttcctaatat cgcaaatagc tacttttcaa ttttagcgcg tggtattgat
    16141 gatattgcag caatgtacaa atataacatt gtccttgctt caagtgatga agacgatgat
    16201 aaagaggtta atgttgttaa tactttgttt gctaagcaag tagacggtat tatttttatg
    16261 ggacaccact taactgaaaa gattcgtgca gagttctctc gttcacgcac accgattgtt
    16321 ttagcaggta ctgtggacct tgagcatcaa ttaccaagtg ttaatatcga ttacaaagca
    16381 gcagcagttg atgttattga tattttagct ggcaatcata aagacattgc ttttgtatca
    16441 ggaccactta ttgacgatat taatggcaag gttcgtttgg caggatacaa agaaggtctc
    16501 aagaaaaatg gcttaaactt taaagagggg ttggtttttg aagccaacta ccgttatgct
    16561 gaaggctttg cattagctca acgcgtgatt aatgctggag caacagcagc atatgttgct
    16621 gaggatgagt tagcggcagg tttgttaaat ggtctttttg aggcaggtaa acgtgtccct
    16681 gaagattttg aaattatcac aagcaatgac tcgccaatcg cacaatatac acgtcctaac
    16741 ttaacttcta tcagtcaacc ggtttatgat ttgggtgctg ttagcatgcg tatgctaaca
    16801 aaaattatgc acaaagaaga gttggaagaa aaagaaatag tgcttaatca cgggattgtt
    16861 aaacgtggca caacaaataa ttagtgttag agtatgttat ttgaaaaata gtggtagaat
    16921 ggtagtagct tagcgcttaa ctttctgata cttaacatga tatattgaga ggattagggt
    16981 agcttagctt cctaatcttt ttttagaggt gtaatatgac aaatgaattg ataatgcaag
    17041 cttttgagtg gtatttacct agtgatggga atcactggaa gaaattagag gactctatat
    17101 cagaccttaa aaaacttgga gttagtaaaa tctggttacc accagcattt aagggaacta
    17161 gcagtgatga tgtaggatat ggtgtttatg atctctttga tttaggagaa tttgaccaga
    17221 atggaacaat tagaacaaaa tatggtagga aagaagagta tctaaagctt attaagtcgt
    17281 taaaggcaaa tggcattaaa ccatttgcag atatcgttct taaccataaa gccaatggtg
    17341 atcataaaga aaaatttcaa gtcataaaag tcaatcctga aaatcgtcaa gaagcattaa
    17401 gtgaacccta tgagattgaa ggatggacgg gatttgattt cccaggtaga cagggtgagt
    17461 acaatgattt taaatggcac tggtaccact ttactggttt agattacgat gctaaaaata
    17521 atgaaacaga catctttatg attgtaggcg ataataaagg ttgggcagac gatgatttga
    17581 ttgatgatga aaatggtaat ttcgattatc tcatgtacaa tgacattgat tttaagcatc
    17641 ctgaagttat aaaaaactta caagattggg ctaagtggtt tattgaaact accggtattg
    17701 aaggttttcg tttggatgct gtgaaacata tcgattctta ctttattcaa actttcatta
    17761 atgatattcg tacaaaaata aaacccgatt tagaggtctt tggcgaatat tggaaatctg
    17821 accaaacatc aatgaaggac tatttagagg caacacaatt tcaattttct cttgttgatg
    17881 ttaccttgca tatgaatttt tttgatgcca gtcaccaaaa ccgtgatttt gatatgcgaa
    17941 caatttttga tgatagtctt gttattgata atcctgagta tgctgtcaca ttcgtagaaa
    18001 atcacgatac gcaaagtgga caagctctag aaagtagagt ggaagattgg tttaaacctt
    18061 tagcttatgg cttgatactt ttacgccaac aagggactcc atgtcttttt tatggtgatt
    18121 attatggcat tcaaggagaa tttggccaac catcctttaa agaggttatt gataaaatgg
    18181 ctgagctacg ccaaaactat gtatttggca aacaagtcga ttattttacg cattcaaatt
    18241 gtattggttg gacttgtctt ggggatgagg aatataattc ttgtttagct gtggtcctaa
    18301 caaatggtga tcagggatgg aaacatatgg aagtagggga aatttatgca ggcaaaacgt
    18361 ttgttgatta tttaggtaat tgtgaacaag aggttgttat aggagatgat ggttggggtg
    18421 attttttagt tgaatctgcg tctatctcgg cttgggtccc taaaatagag gaagctaact
    18481 gataaatcag gtgaaaaaaa aggtaaaatg agatataata agacctatga aagttttact
    18541 gtatttagag gctgaagaat acttgaaaaa gtcaggtatc ggtcgtgcta tcaaacatca
    18601 agagaaagct cttcaaattg ctgggataga ttataccact aatcctgcag atgactttga
    18661 ccttgttcat atgaatacct atggtattag aagttggctt ttgatgagta aagctaaaaa
    18721 aactgggaaa aaagtaatta tgcatggtca ttccactgaa gaggacttta gaaattcttt
    18781 tattggctca aatttagtat ccccgctttt taaatggtac ctttgccgtt tttatcaaaa
    18841 agcagatgcc attataaccc caactgatta ttctaaacaa ctaataaaag catatggcat
    18901 caagaaacca atttttgttt tatctaacgg gattgatcta agtcgctatc agaggtcaga
    18961 aaagaaagaa gctgcatttc gtcactattt tcacttgagt aaagatgata aagttgttat
    19021 gggggccggt ctttatttca tgcgtaaggg aattgaccaa tttgttgaag tagctgcaaa
    19081 aatgcctgat attcgtttta tttggtttgg agaaacaaat aagtgggtta ttcctcgaaa
    19141 ggttaggcaa atcgttacca agcaacatcc ctcaaatgtt acttttgctg gttacataaa
    19201 aggtgacgtt tatgaaggtg ccatgagtgc tagcgatgct ttcttctttc cgagtcgtga
    19261 agagacagaa gggatagtag ttttagaggc tttagctagt catcagcatg tggttttaag
    19321 agatatccct gtgtatcacg gttgggtgac cgaagacagt gttgaattag cgactgatgt
    19381 tgatggtttt gttgaaaaat tggataaagt cttatctggt aagagtgata agattaaaga
    19441 aggttatcat gtggctgaga gtcgaagtat cgaacgaatt gcccatgaat tggcaagtgt
    19501 ttatcaaaaa gtaatggagt tgtaagatgc gtataggtct atttacagat acatacttcc
    19561 cccaagtttc gggagtttca acaagtatcc gcactttaaa ggaagggttg gagaaggaag
    19621 gacacgaagt ttatattttc acaactaccg accgtaatgt caaacgtttt gaagacccta
    19681 caattattcg tttaccaagt gtccctttta tttcttttac ggatcgtcga gtagtttata
    19741 gaggtttaat ttctgcttat aggatagcta aagactacga acttgatatt attcatactc
    19801 aaacagaatt cagcttaggg cttttaggaa aattagttgc aaaagcttta agaatacctg
    19861 ttgtgcatac ttatcatact caatatgagg attacgttgg ttatatcgca aaaggcaaat
    19921 tgattaagcc aagtatggtc aaatatatca tgcgtaccta cctaagtgat ttggatggtg
    19981 tcatatgtcc ttctcgaatt gttctcaatc ttctagatgg ttacggtgtc aaaattccaa
    20041 aacaagttat tccgacaggt attcctgttg agaactatcg tcgagaggat atctctgaag
    20101 aaacaatcaa gaatctacga acggagcttg gacttgcgga taatgataca atgctattga
    20161 gtttatcacg ggtatctttt gaaaaaaata ttcaagcaat cttgatgcat ttatcagctg
    20221 tcgttgatga aaatcctcac gtaaaattag tgatagttgg agatggtcct tatctttcag
    20281 atctaaaaga acttgttcac tctctagaac ttgaaaattc agttattttt acaggtatgg
    20341 ttgagcatag ccaagtggct atctactata aagcatgtga tttctttatt agtgcttcaa
    20401 caagtgaaac gcaaggatta acctacattg agagtctggc gagtggacgt ccaatcattg
    20461 cgcaaagtaa tccttatcta gatgatgtta tttcagataa aatgtttgga actctgtaca
    20521 agaaggaaag tgatttggca gatgctatcc tagatgcaat agcagagaca cccaaaatga
    20581 ctcaagaagc ttatgaacag aaattatatg agatttctgc tgagaatttc agcaagtctg
    20641 tatatgcttt ttacctagac tttctgattt cacaaaaagc tagtgtgaaa gaaaaagtct
    20701 ctctgacaat aggaaataag gatagccatt ctactcttcg ttttgtcaga aaagcggtat
    20761 atttacctaa gaaagttttt acctttacag gaagagcctc taaaaaagtg gtaaaagcac
    20821 ctaaacgtcg tatttcatct attcgagatt ttttagattg agacgaataa aattagtaaa
    20881 tgtcttgaaa ttataaaaaa tattgttata ataaaaaatg aagacatgtt aggttctaat
    20941 agattttaag agcgagcact ggttggtgga aagagtgcaa ttagagaact tagtactact
    21001 tccttgattt tatgcaaaca taaaagggtg gttcccttat cgccactaga ggtctgacac
    21061 aagtatactc gagtttttgt gttggataaa ttgaggtgga accacgtagt ctttacgtcc
    21121 ttttggatgt aaaggctctt tttttataaa agaaatggtt gcataaaaga ggcagattgc
    21181 tttactaatg ttatacttat tatccctagc taggataatg tggaattcaa tcaaaggtca
    21241 atccctttgt ttattagtga attaatgtgt ttaggaattg gtatcaccct aacagttatt
    21301 aagaaaagga gaacaagatg attaaaatca ctttcccaga tggtgcaatt cgtgaatttg
    21361 aatctggcat cacaacattt gaaattgcac aatcaatttc taactcttta gctaaaaaag
    21421 ctttagctgg taaatttaat ggtcaactta ttgatacaac acgtgctatt gaagaagatg
    21481 gtagtattga gattgtaaca cctgaccacg aagatgcttt aggagttctg cgtcactcag
    21541 cagcgcattt atttgctcaa gctgcaaaac gcctttttcc agacttatgt ttaggtgtag
    21601 gtccagctat tcaagatggt ttttactatg atactgataa taaatcaggt caaatttcaa
    21661 atgatgatct tcctcgtgtt gaagaagaaa tgaaaaaaat tgttaaggaa aatcaccctt
    21721 gtattcgtga agaaatcagt aaagaagaag cgttagaact ttttaaagat gacccttata
    21781 aagttgaatt aattagtgaa catgctgaag atggtttaac tgtttatcgt caaggtgaat
    21841 ttgtagacct ttgtcgtgga cctcatgtcc catcaacagg acgtattcaa gtctttcatc
    21901 ttctaaatgt cgctggtgct tactggcgtg gtaacagtga taatgctatg atgcagcgtg
    21961 tctatggtac tgcttggttt gataaaaaag atttgaaagc atatcttaaa cgccgtgaag
    22021 aagctaagga acgtgaccat cgcaaattag gtaaagaact tgatttattt atggttaatc
    22081 ctgaagtagg tcaaggattg ccattctggt tgccaaatgg agcaactatt cgccgcgaat
    22141 tggaacgtta cattgttgat aaggagattg cttcaggtta tcaacacgtt tacacacctc
    22201 cgatggcatc tgttgaattt tacaagactt ctgggcactg ggaccactat cgtgaagata
    22261 tgttcccaac aatggatatg ggagatggag aggaattcgt tcttcgtcca atgaactgcc
    22321 cacaccatat tgaagtttat aaacatcatg ttcactcata ccgtgaattg ccaattcgta
    22381 tcgctgaact tggtatgatg caccgttatg aaaaatcagg ggccctcaca ggattgcaac
    22441 gtgtacgcga aatgacactc aacgatgccc atattttcgt tacacctgag caaattaaag
    22501 atgaatttct taaagctctt aatctcatag cagaaattta tgaagatttt aacttaacag
    22561 attaccgttt ccgcttatct tatcgtgacc cagaagataa acataagtat tatgataacg
    22621 atgagatgtg ggaaaatgca caagctatgc taaaagaggc tatggatgat tttggattag
    22681 attattttga agcagaaggc gaagcagctt tttacggtcc taaattggat atccaggtga
    22741 aaactgctct tggtaatgaa gaaacattat ctactatcca actagatttc cttcttccag
    22801 aacgctttga tcttaaatac atcggtgcag atggtgaaga acatcgtcca attatgattc
    22861 accgcggtgg tatttcaaca atggagcgat ttacagctat tttaattgaa acttacaaag
    22921 gtgcctttcc aacttggctt gctccacaac aagtttctgt tattccaatt tcaaatgaag
    22981 cccacattga ttatgcttgg gaagtggctc gtgtccttaa agatcgtggt attcgtgctg
    23041 aagtggatga ccgtaacgaa aagatgcaat ataagattcg tgcggctcaa acccaaaaaa
    23101 ttccatatca attgattgtt ggtgataagg aaatggaaga aaaagccgta aatgtgcgcc
    23161 gctatggaag caaagcgact gaaacaaaat ctatagagga atttgtagaa agtattttag
    23221 ctgatattgc aagaaaatct agacctgacg aagttaaata aattttatta gtcattataa
    23281 aaatcattaa taaatgatgt gaactgatta ctgtatgata cagaatcagt tttttagatg
    23341 ctaatttttt ctttatgttt aattataagc taattataga taaataatct catataacat
    23401 tcacaaaatt agattttttt ggtataatta atatgattga cacataaagg aaaatagtat
    23461 taataaaaag gggtatggag cattgcgaac agtattagta gtgcaaggtg atgacgaaac
    23521 aatagagtta ttaaggtcgt atttagaggg agctctttat aaggttgtga tggctagtga
    23581 tggtgaggag gctttttctt tatttcagca acatcagatt gatttagcaa tcatagatat
    23641 tacgctacct aaaatagatg gatatgagtt gacgcgactc attcgtcaag atagtcagat
    23701 tcctattatt atgctagcag ctaagacaac agatatggac agaatattag gattgaatat
    23761 tggagcagat gattttatca cgaaaccgtt taattctttg gaggttttag ctcgaattaa
    23821 ttcgcaactg cgtcgttact atgaattcaa tagtcttgcc aaacctaaaa atcaatttat
    23881 aaagataggg gaattggaat tagatgagga acatgttgaa ctaactaaaa atggtaaaca
    23941 tatcaagttg actgctacag aatttaagat tcttcatatc ttaatgtcat caccaggaag
    24001 gatttatact aaaacacaac tctatgagaa gattaatggt aggtatttag aaggtgatga
    24061 gacaacgatt atggtgcata tttctaatat ccgtgacaaa atcgaagatg atagtaaata
    24121 tcctaaatat ataaaaacac ttagaggagt aggatataaa atcgaaaaac cgcagtatct
    24181 tgtttaaaaa tataatggct agtaagagcc attttttagt gaagatattt cttggtttta
    24241 agctttcctt tttgctataa taagtaaaat tggagaggag agatatggca aagaccgctc
    24301 ttaatattcc ttttacagtt gaagggatag aaaattatta tcaaaatata aaaatgatac
    24361 tgtctttagc agataaatgg agcataccta aagatgatct attactcttt ttaaagggac
    24421 tcaaggctat tgatgatata aaagttggct ctcctatttt taagtatttt tggacttctt
    24481 taagtttaca agcacccctc aaagctttag aattcgtgtt agagcaggct aaaatgccaa
    24541 ctgaattgtc aggagaatta tcagaaacac aatacttggt cgcacagttt tcagatgagt
    24601 tagctccaca tgatgatttc tggatagcct tatcacaagt tatttatgat tcttttcctg
    24661 ggaatagtct agccgaagat actgtattaa ataggaaact ccatcaattc cgctatttga
    24721 tttctagtca gcaagcacag tatgttcgac gatactttaa agatgttggt atgactgata
    24781 gagatgcttt agtaaactat ctatcatgtt taagagaacc agataatatc gcctactatg
    24841 aatctgctcg tttgcataat aaacgtcgga gaaatggtga aatctttgct ttcccagatg
    24901 atgagccggt tattaatagt aagcttttaa tttcattcca tacggaattt attattgatg
    24961 acaaaggaaa ttttttaaac gagattgacg cagaagtaat aacaagtaat ggcattatca
    25021 atggagctag ctttaattat gcttttaaaa ataatacacg tcataaagaa ttagatgttg
    25081 atcctgtaaa attagaccca aaatttcgaa acgatatgac tagaggttat agatcgccaa
    25141 atctatctag aaggaaatgg tttttcttta aagaagagga ttacgattgt agttatttca
    25201 ataaaaaagg atattacgca tttggaaggc gtagtgctaa gcaatcggtt gataaacaag
    25261 tcaaatactt gaagaaggct gtgcagaaaa tgcgcctcaa ctgatacata tactattaaa
    25321 agataccata atactatggt atcttttaat ttgaggtatt aatgtcaaat ggttcttatt
    25381 gaccacatgt ttttataaac gagtggtcaa aattgaaaaa gtgtctagct tatgttaaaa
    25441 tatagttgaa taatagaaag aaggtgctgt atgattaaga aaaataaagt ttttttagga
    25501 gttcttttag tccttgttgt tatccttgga ggtggtgtat tattttacca atcacaattc
    25561 caaaagacaa ctaatcaagc gttagctatt gcttataaag atgctaaagt ggctaagaag
    25621 gatgtcatac atcaaaaaat tgacaaagaa tttgaaaact ttagagggag ctacgaaatt
    25681 gagttcaata ccaaaagcgc agaatacagc tatcatgtag atgctaagac tggacaaatc
    25741 cttgagcggg acatggataa taatggcttt tcaaaatcga cttctcagtc aagttcatcg
    25801 tcaagtcaaa aatctcataa aataagtcaa gaagaagcca aaaagattgc atttaaagat
    25861 gctaatatag aggaatcaga agtcagtaat cttaagatca aagaagaaat tgaaaatgga
    25921 aaatcagttt atgatattga ctttgtggac ctgaaaaata aaaatgaagt tgattatcaa
    25981 attgatgcag aaactggaaa aatcattgaa cgttctagag atcatatgaa tgattaattt
    26041 aaataagata taaaaaaacg aaggtctaaa aggccttcgt tttaattact cagtagcttg
    26101 ctcccaacgt tttgaaagtt tacgtgaaaa acttgaaatg ataaagtttg taataaaata
    26161 gacagcagtt ataatagcgt agagtgtaaa aacttgtcca gcttcgaaat atcttcccat
    26221 tagaatttga ctttttccga agatttcttg aatagctata acagaataaa gtagtgatgt
    26281 atcttttata accgttacaa actgagaaat aatggctggt aacattttgc gaaaagcttg
    26341 tgggaaaata ataataatga aaacttgcaa ataagtgaat ccttgagaaa gtccggcttc
    26401 tgtctgtcct ttatcgactc catttaaacc tccccgaata atttctgcta aagcagcgga
    26461 ggtaaaaatt gtaaacgaag taataccagc gctgactgat ttcatctgga atactaagaa
    26521 aatgataaaa atccaaagta gatttggaac atttctgaca aattcgatat agatactagc
    26581 tattaatttc cagataggat ttttgctatt tctcataatt gctaatagag ttccaaaaaa
    26641 cattgatagt aaaatggaaa taaaagatat atataaggtt aaaccgaacc cttgaaggag
    26701 aaatagtata tttgttagtg ttaagactga tgacatatca aacctcctta agaataagtt
    26761 ttcttattta attcttcttg ttttcttgcc cagcttgcta gtgggaaaca aataacaaaa
    26821 tagaagatag cagctcctgc aaaggctggg atataattag ttgtatcata agcccaggcc
    26881 ttagctacaa acatgatatc ggctccagaa atgattgcta cggtagaggt attcttgatt
    26941 aggttaacaa cttggttggt cattggtggt aaaattgttc tgacagcttg aggcaaaatg
    27001 ataaattgca tggtttgatt agctgtgaat ccttgagcta aagcagcctc tgtttgtcct
    27061 ttagggacag cttcaattcc tgagcgaata acttcagaaa tataggcacc atggtatagg
    27121 ccaacgcaca aaacggcggt gaaaaaagca ctaatcataa catgtccatt actaatgatt
    27181 gctagtccat agtagacaaa aaccatttga actaaaagag gtgtgttttg aaaaacttca
    27241 acatagatac gtgatatcaa cttaccaact ttacttttag aagttgataa accaccagag
    27301 agaattccta atatcagagc aagtaggagt gcaccaaacg aaatacctag agtatataag
    27361 aagcctttaa agaataggtc aaaatggtta aagaaagcgc cccaccttga aatggcaaaa
    27421 ggactgatgt ttaataaagt cataaaaaat cctccctatt ctttttttac tttagcgggt
    27481 ttaagcttaa atttttgata aatgtgtttt aaactaccat ctttttccca tgataccagt
    27541 aacttattaa cgtagggaat caagactttg tcctgtttac tggttgcaat accgtattct
    27601 tgcgtattga acccttcttt taatatggtt gtatgagggc taaggtagcc gctaaggata
    27661 gatttatcaa ctgaaaaagc atcaatacga ttagcgtaca gtgatattgc taattctggg
    27721 aaactcccta attgagcata gctaaatttt agcttgtgtt ctttagcata tttttctaaa
    27781 ttgccttttg tagtggcacc ttgagccaca ccaatgtgtt taccatccaa ttcttttata
    27841 gttttgatat gactattttt acgcaccaaa aatccaattt catcgtgata ataagcttta
    27901 gaaatattat agttagcttt tctttcgggc gtgatagtgt atgttgcgat tagaatatca
    27961 atttgcccat tgtccatcag aggctctctg gtttgtgcag ttgtcggtac aaaaactggt
    28021 ttgacaccta aggatttggc aatttttttg gcaatatcaa tttccattcc ttcgtattga
    28081 ttcgtctcag cattataata accaaagtta ggcacatctt gtttgactcc aactttaagg
    28141 acacccgatt ttttgatttc tgataattga ttagctgacg ttgagttggg ataaaataac
    28201 aggaaaatga aaatacagga taatagatag agcgttaatc tttttcgtct cagcatattt
    28261 cgtcctccta aaggtcagta ctggtatggt tgataatgtt gcttaaaaat tgtttggcac
    28321 gtggttctgt ggggttatca aaaaagtctt gtacatccgt agtatccact aaaatttccc
    28381 catcagccat aaaaataata cgatcagcaa cttctcgtgc aaatcccatt tcatgagtta
    28441 caacgaccat attcattccg tcatttgcca atttttgcat cactgaaaga acgtctccga
    28501 tggtttcggg atcaagggca gaggtaggct catcaaagag gaggagtttt ggatgcatgg
    28561 caagccctct agcaatagca attctttgtt tttgaccacc cgatagcata gatgggtagg
    28621 aatcttttct ttcccacata ttgacaaatt ttaaatattt ttcagcaatt tccatagctt
    28681 cttttttaga ttgttttaat actttaatag gagctaatgt aatattttct aacacggttt
    28741 tgtggggata aaggttaaaa tgttgaaaaa ccataccaac ttctttacgg aggttgacca
    28801 attctttaga tgaactattt gctaattcat gtccattcac gaccaaacta ccatcgtcaa
    28861 tagattcgag agcgttcatt gttctaataa gtgttgattt acctgatcca gaaggtccta
    28921 atagaacaac cacttgccct ggctctattt ccaaattaat ctgacgaagg gcgtgataat
    28981 ctccataata cttgttaaca ttcttataag atatgagtga cataagaacc tcctaaaata
    29041 acagtttatt gttaggttac ctaacattat agataagtgt atcacctgtt tgtgagcttg
    29101 tcaataaatt cagaatatta aagagtagga ttgatttaaa tattgctatt tttagagcgg
    29161 tatttttagt aaaaatatta cgttctcgta tcatttattt ttcagtttaa taggatgtat
    29221 tgaaagtttc caattaacta ggaaatctgt tataattagt gataatagtg gtgctttagg
    29281 cctatattat catattttcg gtcactttta tttttcagtt tatgaatgga agtgggagac
    29341 cagaaagtaa tagaggtaaa atatgaaaaa aatacttatc gttgatgatg aaaaaccaat
    29401 ttcagatatt atcaaattta atttaaccaa agaaggatat gagacggcga cagctttcga
    29461 tggccgtgaa gctttagtac aatatgcaga atttcaacca gatttaatca ttttggattt
    29521 aatgctccct gaactagatg gtttagaagt ggctaaagaa gttcgtaaaa ccagccatat
    29581 tcctattatt atgctttctg caaaagatag tgagtttgac aaagtcattg gtttagaaat
    29641 cggtgcagat gattatgtta ctaaaccttt ttcaaatcgt gaacttttgg cacgggtcaa
    29701 agcacattta cgacgtactg aaaatattga aacagctgta gcagaagaaa gtgctcaaaa
    29761 tgcttcttcg gatattacta ttggtgagtt gcaaatttta ccagacgcct ttattgctaa
    29821 gaaacgtggc gaagaaattg aattaacgca ccgagaattt gaattacttc accatttagc
    29881 gacacatatc ggacaagtca tgacacgcga acatttattg gaaacagttt ggggatatga
    29941 ttattttgga gatgtccgta cagttgatgt gacggtgaga cgtttacgtg aaaagattga
    30001 agatactcct ggtcgtccag aatatatttt gactcgacgt ggtgtaggtt attacatgaa
    30061 gtcttatgaa taatagtgct gcaaatatta ggtcgtttga gctagcctta ctttttttac
    30121 tagtttttgt tgctgtctat tttgtttatc tagcggttag agattttaaa atgtctaaaa
    30181 atatccggct tttgaattgg aaagtcagag atttgatagc aggaaattat tcagatagta
    30241 ttctaattca aggtgatgca gatttggtag aattagggga aagtctaaat gatttatctg
    30301 atgtcttccg aatggcacat gataacttag aacaagaaaa gaatcgctta gcaagtatct
    30361 taacttatat gactgacggt gtgctggcta cagatcgttc aggaaaaatc gttatgatta
    30421 atgagacagc ccaacagcag ttcaatctag catatgatga agctttatca atgaatattg
    30481 ttgatatgtt agggtcggga agtccatatt cgttccaaga tttagtctct aaaacgccag
    30541 aggtagttct taatagacgt gatgaaaatg gtgaatttgt taccttacgg attcgatttg
    30601 ctttgaaccg tcgtgaaagt ggttttattt ccggtttagt tgcagtatca cacgatgcta
    30661 ctgagcaaga gaaagaagag cgtgaaagaa gactttttgt atctaatgtt tctcatgaac
    30721 ttcgtacacc tttgacatct gtaaaatctt atttagaagc tttagatgag ggagcgctca
    30781 atgaagaagt tgctccaagc ttcattaagg tatctctaga tgaaacaaat cgtatgatgc
    30841 gtatgatatc agatttatta agtttatcgc gcattgataa tgaagtaacg catttagatg
    30901 ttgaaatgac gaattttaca gctttcatga cctcaatttt gaatcgattt gaccagatta
    30961 gaaatcaaaa aacagtcaca ggaaaagttt atgaaattgt cagagattat cctcttaagt
    31021 caatttgggt ggaaattgat acagataaaa tgactcaagt gattgataac attttaaata
    31081 atgcagtcaa gtattcacca gatggtggta agattacagt taatctacgc acaactaaaa
    31141 cgcagatgat tttatcaata tcagaccaag gcttaggtat tcccaaaaaa gatttacctc
    31201 tcatctttga tcgtttttat cgtgttgata aggcgagaag tcgtcaacag ggtgggactg
    31261 gacttggttt gtcaattgca aaagaaattg ttaagcagca taagggattt atttgggcta
    31321 agagtgagta tggtaaaggg tctactttta caatcgtctt gccttatgat aaagatgctg
    31381 taacttatga agaatgggag gacgttgaag attaaaacat gtcagaaata ggatttaaat
    31441 atagtatctt agcttcaggt tcaactggaa attgttttta tattgagaca ccccaaaaac
    31501 gtcttttaat cgatgctggt ttaacaggca agaaagtgac tagccttctt gcagaaatta
    31561 atcgtaagcc agaagattta gatgctatac tagttaccca cgaacattct gaccacatta
    31621 aaggtgttgg cgtattggct aggaaatatc accttgatat ttatgctaat gagcagacgt
    31681 ggaaagttat ggatgagcgt aacatgttag gcaaagttga tgttagtcaa aagcatgttt
    31741 ttggacgcgg tacaacctta actttcgggg atttagatat tgagagcttt ggtgtaagtc
    31801 atgatgctgt ggatccccaa ttttaccgta tgatgaaaga tgataagagc tttgtgatgt
    31861 tgacagatac aggatatgtt agcgaccgaa tggcaagctt gattgagaat gcagatggtt
    31921 atttgatcga gtcaaatcac gacattgaga ttctacgttc gggttcttat ccttggacac
    31981 ttaagcaacg tattttatca gataaaggac atttatctaa tgaagatggt tcagaaacca
    32041 tgattcgcac tataggtaac cgtactaaac acatctattt aggacacttg agtaaggaaa
    32101 ataatattaa ggaacttgct cacatgacta tggaaaacaa tttaatgaga gctgactttg
    32161 gtgtgggcac agatttttct gttcacgata cttcccctga ttcagctacc ccactgacga
    32221 ggatttaaga ttgaaaaaga ttttttacat tagtttagga tttattagtt tagggatagg
    32281 cattgcaggt attgtcttgc cggtagttcc aacgactcca ttagtattgc tttcagcttt
    32341 ttgcttttcg agaagctctg aaaaatttga tatttggttg cgtcaaacta aagtttataa
    32401 atactacgct gcagattttg ttgaaagcag aagtattgcc ccagcgcgta aaaagtcaat
    32461 gatttggcaa atttatatct tgatgggaat ttcaatctat tttgctcctc taatgtggct
    32521 caaactggga ttattgattg gaactattgt aggaacttat gtgctttttt atgtagtacc
    32581 tgataaaatt gatgaatagt taacaagaga ctaggtgtgt atcctagttt tttgttgaat
    32641 attcaaatat gtaattaagg aa
//
LOCUS       DATRNK010000018        30665 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.018, whole genome shotgun sequence.
ACCESSION   DATRNK010000018 DATRNK010000000
VERSION     DATRNK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30665
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.018"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            1..504
                     /locus_tag="VBC52_000829"
     CDS             1..504
                     /locus_tag="VBC52_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882429.1"
                     /translation="MKLNKIILSTAALTALFLGYNSVTADTYNNYQPHRSNNMDLTEE
                     YNYNNQIELQERIKKLNIPFKEKLALLRKVKSRQDSYVLDTLRKEVENKNSEYSELEQ
                     EYQRISALIDAKFPQGKSSLAEKLHKELIWDLDGVKYDEGISKRTALKKLEGKINEYT
                     KKYSYLF"
     gene            complement(556..741)
                     /locus_tag="VBC52_000830"
     CDS             complement(556..741)
                     /locus_tag="VBC52_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882430.1"
                     /translation="MDNCTLEFPYSFENNFKPILSAFTSNEMPSYPMLPLCLQKAPPS
                     LIGQKKTPQKGVFLVSI"
     gene            813..1538
                     /locus_tag="VBC52_000831"
     CDS             813..1538
                     /locus_tag="VBC52_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6882431.1"
                     /translation="MLKIENVTGGYVNIPVLKNISFEVNDGELVGLIGLNGAGKSTTI
                     NEIIGILRPYQGDITIDGISLEADQELYRKKIGFIPETPSLYEELTLREHLETVAMAY
                     DIATDEVMARAQKLLEMFRLTDKLDWFPMHFSKGMKQKVMIICAFVVSPSLFIVDEPF
                     LGLDPLAISDLINLLAEEKAKGKSILMSTHVLDSAEKMCDRFVILHKGEIRAVGTLEE
                     LRAIFGDSNANLNDIYIALTKEV"
     gene            1540..2574
                     /locus_tag="VBC52_000832"
     CDS             1540..2574
                     /locus_tag="VBC52_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6882432.1"
                     /translation="MKKLFNKRRSLFLTQNSKYLRYVFNDHFVLVLMFLSGFLLYQYS
                     QLLKDFPKTHWPIIVIVSIIILMLLAMGGIASYLEPADKQFLLIKEEAIKEIINSAKK
                     RTYIFWLVIQTLFLVLISPILIKLGLSVFMITLLIFGLGIIKWLVITYKVKVFYNNQN
                     LNWDAAINHEQERKQSILKFFSLFTNVKGISTSVKRRSFLDGILKLISKTPSRLWTNL
                     FVRAFLRSSDYLGLTIRLVTLNILSVIFVNETYLALALAFVFNYLLLFQLLALGHHFD
                     YQYMNQLYPVRLNAKASQLKGFLRVLSYAVTVIDSILIRELKPVILLIVLMLIVTEYY
                     IPYKIKKMID"
     gene            2632..3432
                     /locus_tag="VBC52_000833"
     CDS             2632..3432
                     /locus_tag="VBC52_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEO6882433.1"
                     /translation="MTISNQELTLTPLRGKSGKAYIGTYPNGERVFVKYNTTPILPAL
                     AKEQIAPQLLWARRTSNGDMMSAQEWLDGRTLTKEDMGSKQIIHILLRLHKSRPLVNQ
                     LLQLGYKIENPYDLLMDWEKQTPIQIRENTYLQSIVTELKRSLPEFRTEVATIVHGDI
                     KHSNWVITTSGLIYLVDWDSVRLTDRMYDVAYILSHYIPQKHWKDWLSYYGYKDNEKV
                     WSKIIWYGQFSYLSQIIKCFDKRDMEHVNQEIYELRKFRELIKKHNAS"
     gene            3422..4057
                     /gene="trmB"
                     /locus_tag="VBC52_000834"
     CDS             3422..4057
                     /gene="trmB"
                     /locus_tag="VBC52_000834"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEO6882434.1"
                     /translation="MRVRKRKGAEEHLENNPHYVISNPEEAKGRWHEIFGNNNPIHIE
                     VGSGKGAFITGMAEQNPDINYIGIDIQLSVLSYALDKVLDSGAKNIKLLLVDGSSLSN
                     YFDTGEVDLMYLNFSDPWPKKKHEKRRLTYKTFLDTYKDILPEQGEIHFKTDNRGLFE
                     YSLASFSQYGMTLKQVWLDLHASDYQQNIMTEYERKFSNKGQVIYRVEARF"
     gene            4101..4188
                     /locus_tag="VBC52_000835"
     tRNA            4101..4188
                     /locus_tag="VBC52_000835"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4135..4137,aa:Ser,seq:gga)
     gene            4547..5023
                     /gene="rimP"
                     /locus_tag="VBC52_000836"
     CDS             4547..5023
                     /gene="rimP"
                     /locus_tag="VBC52_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEO6882435.1"
                     /translation="MANQTIVDIVTQVVTPAIQAPFELVDVEYEKMGGDYVLSILIDK
                     PGGITVEDTAQLTDVVSPLLDTIQPDPFPEQYMLEVSSPGLERPLKTAEALSNAVGSY
                     INVSLYKSIDKVKIFEGDLLSFDGETLTIDYMDKTRHKTVDIPYQTVAKARLAVKL"
     gene            5050..6210
                     /gene="nusA"
                     /locus_tag="VBC52_000837"
     CDS             5050..6210
                     /gene="nusA"
                     /locus_tag="VBC52_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEO6882436.1"
                     /translation="MDTMSKEMLEAFRILEEEKHINKEDIIDAVTESLKSAYKRRYGQ
                     SESCVIEFNEKKADFTVYTVREVVDEVFDSRLEISLKDALAISSAYELGDKIRFEESV
                     TEFGRVAAQSAKQTIMEKMRRQMREVTFNEYKQHEGEIMTGTVERFDQRFIYVNLGSL
                     EAQLSHQDQIPGESFKSHDMIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEREIPEV
                     FDGTVEIMSVSREAGDRTKVAVRSHNSNVDAIGTIVGRGGSNIKKVISNFHPKRVDAK
                     TGLEIPVEENIDVIQWVEDPAEFIYNAIAPAEVDMVLFDDEDTKRATVVVPDSKLSLA
                     IGRRGQNVRLAAHLTGYRIDIKSASEYEKMEAQELQTEEVAQESEVISDKAE"
     gene            6232..6528
                     /locus_tag="VBC52_000838"
     CDS             6232..6528
                     /locus_tag="VBC52_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEO6882437.1"
                     /translation="MAKTKKIPLRKSVVSGEVIDKRDLLRIVKNKEGQVFIDPTGKQN
                     GRGAYIKLDNDEAILAKKKRVFDRSFSMEVSDEFYDELLAYVDHKVKRRELGLE"
     gene            6521..6823
                     /locus_tag="VBC52_000839"
     CDS             6521..6823
                     /locus_tag="VBC52_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEO6882438.1"
                     /translation="MNNSEKVLNLIGLAQRAGRLITGEELVIKAIQNQQVSLIFLAND
                     AGPNLTKKVTDKSNYYKTEVSTVFSTLELSDALGKPRKVVAVADAGFSKKMRTLMA"
     gene            6843..9626
                     /gene="infB"
                     /locus_tag="VBC52_000840"
     CDS             6843..9626
                     /gene="infB"
                     /locus_tag="VBC52_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEO6882439.1"
                     /translation="MSKKRLHEIAKEIGKTSKEVVEQAQSLGLPVKSHASSVEENDAT
                     RIVESFSSSKTKAPTNSVQTNQGVKTESKTVETKQGLSDDKPSTQPVAKPKPQSRNFK
                     AEREARAKAEAEKRQHNGDHRKNNRHNDTRSDDRRHQGQKRSNGNRNDNRQGQQNNRN
                     KNDGRYADHKQKPQTRPQQPAGNRIDFKARAAALKAEQNAEYSRHSEQRFREEQEAKR
                     QAAKEQELAKAAALKAQEEAQKAKEKLASKPVAKVKEIVNKVAATPSQTADSRRKKQT
                     RSDKSRQFSNENEDGQKQTKNKKNWNNQNQVRNQRNSNWNHNKKNKKGKTNGAPKPVT
                     ERKFHELPKEFEYTEGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGDTIELL
                     MVDYGIEAHAKVEVDEADIERFFADEDYLNPDNLTERPPVVTIMGHVDHGKTTLLDTL
                     RNSRVATGEAGGITQHIGAYQIEEAGKKITFLDTPGHAAFTSMRARGASVTDITILIV
                     AADDGVMPQTVEAINHSKAAGVPIIVAINKIDKPGANPERVISELAEHGVISTAWGGE
                     SEFVEISAKFGKNIQELLETVLLVAEMEELKADADVRAIGTVIEARLDKGKGAVATLL
                     VQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGDHFAV
                     YADEKAARAAGEERAKRALLKQRQNTQRVSLENLFDTLKAGEVKSVNVIIKADVQGSV
                     EALAASLLKIDVEGVKVNVVHSAVGAINESDVTLAEASNAVIIGFNVRPTPQARQQAD
                     ADDVEIRQHSIIYKVIEEVEEAMKGKLDPEYQEKILGEAIIRETFKVSKVGTIGGFMV
                     INGKVTRDSSVRVIRDGVVIFDGKLASLKHYKDDVKEVGNAQEGGLMIENYNDLKEDD
                     TIEAYIMEEIKRK"
     gene            9717..10085
                     /gene="rbfA"
                     /locus_tag="VBC52_000841"
     CDS             9717..10085
                     /gene="rbfA"
                     /locus_tag="VBC52_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEO6882440.1"
                     /translation="MKKGLIMANHRIDRVGMEIKREVNEILRLRVNDPRVQDVTITDV
                     QMLGDLSMAKVFYTIHSTLASDNQKAQIGLEKATGTIKRELGKNLTMYKIPDLQFVKD
                     ESIEYGNKIDEMLRNLDKKD"
     gene            complement(10169..11173)
                     /locus_tag="VBC52_000842"
     CDS             complement(10169..11173)
                     /locus_tag="VBC52_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6882441.1"
                     /translation="MKPSFKKLLLLFSIITILSIACTPHAKASGRSWKSWFIEQYFWL
                     KRDKSYYKVQDESSFQKYLNASREQSDKGYYLDPNSVNGGLVQERLFDMQVYSWNDNG
                     KANQKTIFYLAGGSYLNNPTPYHISMLKTLSTSLDAKIILPIYPKTPRYTYDYAIPRL
                     VNLYRHFHEKNANLTLMGDSAGGGLALGLAHALSHQSGQEAIPQPKNIILLSPWLDVT
                     MKHPEIPKYEDTDPILSAWGLARVGEIWANGSNNTNHTYVSPKNAPATKLAPITLFTG
                     TREIFFPDIRDYAAQLQAANHPVNYIAQEGMNHVYPIYPIEEAKTAQYQMIDIINKTP
                     "
     gene            11337..11753
                     /locus_tag="VBC52_000843"
     CDS             11337..11753
                     /locus_tag="VBC52_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEO6882442.1"
                     /translation="MTISSAEWEIMRVVWAQQNTTSNEILAVLLEKYDWTPSTVKTLL
                     RRLLDKGYVSREKMGKGFSYSPLIDEDLAMMSEVDSVFQKVCQTKHVAIVRHLLESIP
                     MTEKDRLNLQSSLEAKKGKTLERVACNCIPGQCQCH"
     gene            11766..14000
                     /locus_tag="VBC52_000844"
     CDS             11766..14000
                     /locus_tag="VBC52_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEO6882443.1"
                     /translation="MANRKETFLIDGMTCASCALTIEKAVNKLDHVDSAVVNLATEKM
                     TVTFDDTTLSPNVIEECVSESGYEASLFKEETSKSQSERHQLAIEKMWHRFWMSAVAT
                     IPLLYISMGPMINLWLPSFLMPDKGPLNYGMIQLLLTLPVMYFGRIFYQNGFKALFKR
                     HPNMDSLVAIATTAAFIYSLYGLYEILQGDIHYAHQLYFESVAVILTLITLGKYFEIL
                     SKGRTSASIEKLLTLSAKEARVIKDGEDYMVPLDKVKIGETILVKPGEKIPLDGHVVA
                     GESSIDESMLTGESIPVEKKVGSKVYGASINGQGSLTIFVEKEAGGSLLSQIINLVEA
                     AQTSKAPIANLADKVSGVFVPFVIVIAILSGLSWYLILGQSFAFSLKIMIAVLVIACP
                     CALGLATPTAIMVASGKAAENGILFKGGEVLEKAHHIDTIVFDKTGTLTKGKPEVVAI
                     KTYGGDKEEFLGQVASVEKLSNHPLSQTIVNKAKEKELPLREVMAFKNILGYGLSATI
                     NGKTMLVGNANLMTKNDVNLDLAKADIEIAQEEAQTVVYVSENGVLSGLITLTDQLKT
                     DSQETVKQLQRLGFNLVLLTGDNKASADAIAQKLGITTVVSEVLPDQKANVILELKEK
                     GGQIAMVGDGINDAPALASSDVGISMSSGTDIAIESADIVLMKPELTDLLKAMTISKQ
                     TIQIIKENLFWAFFYNVLAIPVAMGVLHLFGGPLLNPMLAGLAMAFSSVSVVLNALRL
                     KVLK"
     gene            14041..14247
                     /locus_tag="VBC52_000845"
     CDS             14041..14247
                     /locus_tag="VBC52_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEO6882444.1"
                     /translation="MKHTYRVSGMKCDGCAKTVSDKLSSVIGVDEVNVDLTKNQVVVS
                     GKTFKWLLKRSLKDTKYSLEEEIN"
     gene            14357..14971
                     /locus_tag="VBC52_000846"
     CDS             14357..14971
                     /locus_tag="VBC52_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEO6882445.1"
                     /translation="MSIDIWNILSLVGTVAFASSGAIVAIEEEFDILGLFILGFVTAF
                     GGGAIRNVLIGLPIETLWSQGIAFYAAAAAILFIMIFPNLLSGKGRDAEVVSDAIGLA
                     AFSVQGALYATQSHQPLSAVIVAAVLTGAGGGIVRDVLAGRKPGVLRSEIYAGWSILV
                     GIILYFKIAKTTTDYYLLVLVVTSLRMLGYKKQWHLPVVRPRKK"
     gene            14986..15798
                     /locus_tag="VBC52_000847"
     CDS             14986..15798
                     /locus_tag="VBC52_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6882446.1"
                     /translation="MIKLIAIDMDGTLLNDEKKIPKENIQAIKEATQAGIKIVLCTGR
                     PMSGILPYFNELGLTKEEYIIMNNGCSTYSTKDWQLIDSATLTHDELIFLEEVVKEFP
                     NVCLTLTAENTFYAVGEEVPEIVAYDADLVFTKAKSTSLDALRNQEEIVFQAMYMGLD
                     ADVTAFQEAVEEALISKFSGVRSQDYIYEIMPQGVTKARGLKSLIAKLGLDINQVMAI
                     GDAPNDIELLDLVPNSVAMGNASDEIKSRCKYITVDNNKAGVAKAIYDYALR"
     gene            15911..18553
                     /gene="polA"
                     /locus_tag="VBC52_000848"
     CDS             15911..18553
                     /gene="polA"
                     /locus_tag="VBC52_000848"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEO6882447.1"
                     /translation="MTNKNKLLLIDGSSVAFRAFFALYNQIDRFKNNSGLHTNAIYGF
                     HLMLNHILGRVQPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPYIRQQLD
                     VLGIKHYELEHYEADDIIGTLAKQAEASNEHFDITVVSGDKDLIQLTDTNTVVEISKK
                     GVAEFEEFTPAYLMEKMGITPSQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLSEYGS
                     LEGIYENIEAMKQSKMKENLINDKEQAFLSKTLATINIASPITIGLEDILYSGPQDIK
                     ALSQFYDEMDFKQFKAALGEETSQEDFEVDFTEVEQLKTEMFSDNDFYYFEMLGDNYH
                     VEDLIGIAWGNSDTIYATSNVSLLQEALFKKALSKPIKTYDFKRSKVLLNRFNIDLPE
                     PAFDTRLAKYLLSTTEDNLVSTIARLYTNLPLDTDDAVYGKGAKRAIPEKTRFLEHLA
                     KKVKVLVDSEANIMQQLKANEQEELLFEMEQPLANVLAKMEIRGIKVKKNTLNEMAIE
                     NQKVIETLTQEIYELAGQEFNINSPKQLGKLLFETLGLPVEMTKKTKTGYSTAVDVLE
                     RLAPISPLVTKILEYRQITKLQSTYIIGLQDYILEDGKIHTRYVQDLTQTGRLSSSDP
                     NLQNIPVRLEQGRLIRKAFVPSEDNAVLLSSDYSQIELRVLAHISKDEHLIAAFKEGA
                     DIHTSTAMRVFGIEKPENVTPNDRRNAKAVNFGIVYGISDFGLSHNLGIPRKLAKQYI
                     DTYFERYPGIKNYMETVVREAKDKGYVETLFHRRRSLPDINSRNFNIRQFAERTAINS
                     PIQGSAADILKIAMINLDRVLDKGGYKSKMLLQVHDEIVLEVPNEEIGAIRELVTKTM
                     ESAISLSVPLIADENAGETWYEAK"
     gene            18583..19023
                     /locus_tag="VBC52_000849"
     CDS             18583..19023
                     /locus_tag="VBC52_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEO6882448.1"
                     /translation="MVYHFQNPSDFMLKNYLTKAKTIAVVGLSDRQETAAYQVSKIMQ
                     EAGYQIIPVNPKNAGQKILGQMTYASLKDVTEHIDIVNIFRRSEYLPDIAREFLEVDA
                     DIFWAQLGLESQEAETILKQAGHKQIVMNKCLKVECQKLHSETN"
     gene            19105..19584
                     /gene="perR"
                     /locus_tag="VBC52_000850"
     CDS             19105..19584
                     /gene="perR"
                     /locus_tag="VBC52_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEO6882449.1"
                     /translation="MDSHSHGHRPLDAYENVLEHLREKRIRITETRKAIISYMVNSRE
                     HPSAEKIYNDLLPEYPNMSLATVYNNLKVLVDEGFVTELKLCNYSTTYYDFMGHQHLN
                     IACEDCGKIVDFVDVDLLDISREAHQQTGFEVTRVQLVAYGICPECQRKRKDKYKKD"
     gene            19737..21302
                     /locus_tag="VBC52_000851"
     CDS             19737..21302
                     /locus_tag="VBC52_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEO6882450.1"
                     /translation="MKISQYNKWSIRRLKVGAASVMIASGSIVALGQSHIVSADEMSQ
                     PKTTITAPTANTSTNVESSTDKALSKVTTMETSSEMPKMQNMAKVEKTSDKPMMVATS
                     VRKMMATPTPVAMTKTTSVDEVKKSTDTAFKQTVDVPAHYVNAAKGNGPFLAGVNQTI
                     PYEAFGGDGMLTRLILKSSEGAKWSDNGVDKNSPLLPLKGLTKGKYFYQVSLNGNTTG
                     KEGQALLDQIKANDKHSYQATIRVYGAKDGKVDLKNMISQKMVTINIPHITTDMEVKN
                     SLKMAFKEKVDVPAKYVSAAKAKGPFLAGVNETIPYEAFGGDGMLTRLILKASEGAKW
                     SDNGVDKNSPLLPLKDLTKGKYFYQVSLNGNTAGKKGQALLDQIKANGSHTYQATITI
                     YGTKDGKVDMNTILGQKTVMIHINVAKKDMNSTSMMMKKDKMTMPMKKEMTSSKINTG
                     MMMSNNKMSANMQMSSQAKSNDKAGKKMSMMSKNLPNTGETKQQNVGVLGMLSLAFAT
                     GLTALGLKKSKQR"
     gene            21415..22101
                     /locus_tag="VBC52_000852"
     CDS             21415..22101
                     /locus_tag="VBC52_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6882451.1"
                     /translation="MTQKLLLVDDEFEIIDINRRYLEQAGYEVSVAADGIEALKEVDE
                     NRFDLIISDIMMPKMDGYDFISEVLVREPNQPFLFITAKVSEPDKIYSLSMGADDFIS
                     KPFSPRELVLRVKNILRRIYGNHQQSEVLTIGDLVIDQKQRLVMVDCNTISLTNKSFD
                     LLWILANHLNRVFSKTELYERVWGEEFLDDTNTLNVHIHALRNDLAKFSTDNTPTIKT
                     VWGLGYKLEE"
     gene            22103..23140
                     /locus_tag="VBC52_000853"
     CDS             22103..23140
                     /locus_tag="VBC52_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6882452.1"
                     /translation="MKLKYYIVIGYLISMLITVAGVFFGLNHMLIETRGVYYILSVTI
                     IACIVGGIVNLFLLSSVFTSLKKLKQKMKDISQRCFDTKAQICSPQEFKDLETAFNQM
                     SSELESTFKSLNESEREKTMMIAQLSHDIKTPITSIQSTVEGILDGIISEEEVNYYLN
                     TISRQTNRLNHLVEELSFITLETMSDTAEPHKEETIYLDKLLIDILSEFQLVFEKENR
                     QVMIDVAPDVSKLSSQYDKLSRILLNLISNAVKYSDPGSPLTIKAYSNRQDIVIDIID
                     QGYGIKDEDLASIFNRLYRVESSRNMKTGGHGLGLYIARQLAHQLNGDILVESQYQKG
                     SKFSLVLKLQK"
     gene            complement(23154..23894)
                     /locus_tag="VBC52_000854"
     CDS             complement(23154..23894)
                     /locus_tag="VBC52_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEO6882453.1"
                     /translation="MTNSEIKNEAKTILSNLQGKNQLFLLPILLSIITLYISFYYQYN
                     NMTLLDFFVPLPVYFFYTLFIISVSFVMLDVVKNQKLNVRFSDNTYVFSSHIFWKLLS
                     VLVLKGLILSFFYLLSTFGLLIIISSFRLLLSGNLILAPVLIVVSSLITTKAVIKLVQ
                     QYYSYSISTLVFYTQLESGNYEGPSKVLVASRELMNGNKLRLFLLDLSFIGWQFLTIF
                     SFGLVYIYLLPYQTTARLIFYRNITKNS"
     gene            24081..25223
                     /gene="tgt"
                     /locus_tag="VBC52_000855"
     CDS             24081..25223
                     /gene="tgt"
                     /locus_tag="VBC52_000855"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEO6882454.1"
                     /translation="MTDHPIKYRLIKQEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIAKAGGLHKFMNWDQAILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGAKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLNAHRRPHDQGLFGIVQGAGFEDLRRQSARDLVSMDFPGYSI
                     GGLAVGETHDEMNAVLDFTVPMLPNDKPRYLMGVGAPDSLIDAVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKFAEDFTPLDPNCDCYTCKNYTRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKDVRQAIMDDNLLEFRQDFMERYGYGMNNRNF"
     gene            25330..25641
                     /locus_tag="VBC52_000856"
     CDS             25330..25641
                     /locus_tag="VBC52_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882455.1"
                     /translation="MMQEYFGIGLDSSSRCYHYHTKLDIVALKCAVCQKYYACYKCHD
                     ALEEHCFAATKSDETFPVLCGSCRQMLTLKEYKTGFCPYCRMLFNPNCQRHDSIYFSK
                     E"
     gene            25648..26187
                     /locus_tag="VBC52_000857"
     CDS             25648..26187
                     /locus_tag="VBC52_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEO6882456.1"
                     /translation="MFHTKSLIQMAMMATLLVVLGFIPALPLGFIPVPIVLQNLGVMM
                     AGVILGGRKGSLSILLFFLVGLVIPVFSGLRSTIPVLTGPTAGYVIAWLFVPLLISYG
                     VKLVNKKNFIAIFLIVWLAGVLFVDVAGAVWLASYTNIPLDKSLLQNLVFIPGDTIKA
                     IIAAVVAVKYKDSFFLTEK"
     gene            26326..27102
                     /locus_tag="VBC52_000858"
     CDS             26326..27102
                     /locus_tag="VBC52_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6882457.1"
                     /translation="MKLTTLGCWGGYPYKDGGTTSFLLTGENNFHLLIDAGSRSINEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLYHYRLLYPKDKWEPKVLPVYGHNEDDYEFS
                     KLTLPNVAQGIAYHTNGVEQVGPFNITFIKTVHPVVCYAMRITERETGQVLVFTGDSG
                     YFADLVPFTKGADLFLADVYLYEGNENHIAHLTSKEAGEIAYNAKVKKLVLTHVPQFP
                     GEGINPENHLEELRLQSEKYAGGISVDLAQPHKSWDLGKL"
     gene            27102..27608
                     /locus_tag="VBC52_000859"
     CDS             27102..27608
                     /locus_tag="VBC52_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEO6882458.1"
                     /translation="MQKFTQEEKEWFMTEALKEAKSSLEKEEIPIGCVIVKDGHIIGR
                     GHNAREEFNKAILHAEIMAINNANEKVGNWRLLDTTLFVTVEPCVMCSGAIGLARIPH
                     VIYGAKNTKFGAAGSLYDILSDSRLNHRVEVETGILEDQCAKIMQDFFRKGRQRKKEA
                     KLSEKTKI"
     gene            27643..27728
                     /gene="ffs"
                     /locus_tag="VBC52_000860"
     ncRNA           27643..27728
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VBC52_000860"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            27880..29229
                     /locus_tag="VBC52_000861"
     CDS             27880..29229
                     /locus_tag="VBC52_000861"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEO6882459.1"
                     /translation="MTHITFDYSKVLGQFVGEHELDYLQPQVSAADAFLRQGTGPGSD
                     FLGWMDLPENYDKEEFSRIQKAAEKIKSDSEVLVVIGIGGSYLGAKAAIDFLNNHFAN
                     LQTAEERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVVRVEK
                     PRKNVTIPELTEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPTQDAY
                     TLGYTIYFFELAIGLSGYLNSVNPFDQPGVEAYKRNMFALLGKPGFEELSAELNARL"
     gene            29551..30078
                     /locus_tag="VBC52_000862"
     CDS             29551..30078
                     /locus_tag="VBC52_000862"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEO6882460.1"
                     /translation="MEKKLLRKEVLITLKSQPQAYKSEVDCKLLEAFIKTKAYQNSCV
                     IATYLSFDYEYNTQLLIKQALCDGKRVLVPKTYPKGKMIFVDYQKDNLRTTPFGLLEP
                     VNDRAVEKASIDLIHVPGLIFNNKGFRIGYGAGYFDRYLSDFEGDTISTIYRCQRQDF
                     VEEKHDVAVKEVLCL"
     gene            30069..>30665
                     /locus_tag="VBC52_000863"
     CDS             30069..>30665
                     /locus_tag="VBC52_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEO6882461.1"
                     /translation="MSMKKFAKEYPTTVLLVSLTTLVFLLMQLTYGSQAESSQVIFQF
                     GGIQGDYLKAYPTNLWRLISPIFVHIGWEHFLLNGLALYFVGQMGESIWGSLRFLILY
                     ILSGLMGNIFTLFFTPHVVAAGASTSLFGVFSAIAIAGYFGKNPYLKQVGKSYQVMIL
                     LNLFFNIFTPGVSLAGHVGGLVGGVLVAIFLTKQNGSLL"
BASE COUNT        10381 a         4653 c         6140 g         9491 t
ORIGIN      
        1 ttgaaattaa ataaaataat attatctact gcagctctta ctgctctctt tttaggatat
       61 aatagcgtta ctgcggatac atataataac tatcagccac atagatcaaa taatatggat
      121 ttaactgagg aatataacta taataaccag atagaacttc aggagcgtat aaaaaaacta
      181 aatatacctt ttaaagaaaa acttgcctta cttagaaagg ttaaatcaag acaagattct
      241 tatgtattag acacattaag aaaagaagtt gaaaataaaa acagtgagta ttcagaacta
      301 gagcaagaat atcaaagaat ttcagctctt attgatgcta agtttccgca agggaaatct
      361 agtttagcag aaaaattaca taaagaacta atttgggact tggatggtgt taaatatgat
      421 gagggtattt ctaagagaac tgctctaaaa aaattagaag gtaaaatcaa cgaatatact
      481 aaaaaataca gttatttatt ctgataattg agtttgatag ttttatagcc taatattctg
      541 attactttgc atttattata ttgatactaa aaagacacct ttttgaggtg tctttttttg
      601 tccgatgagt gatggaggtg ctttttgtaa acataatggg agcatggggt agcttggcat
      661 ttcattagat gtaaaagcgg acaaaatagg tttaaagtta ttttcaaaac tataagggaa
      721 ttctaaagta caattatcca taagaataga ccttcccttt cttattttgt tataatagtt
      781 aataaagtat atcaaagata gagagaatga caatgttaaa aattgaaaat gttacagggg
      841 ggtatgttaa tatcccagtt ttaaaaaata tttcttttga agtaaatgat ggagagttag
      901 ttggattaat tgggcttaat ggtgcaggaa aatcaaccac tattaacgag attattggta
      961 ttttgagacc ctatcaagga gatattacaa ttgatggtat tagtcttgaa gccgaccaag
     1021 aactttatag aaagaaaata ggctttattc ctgagacacc aagtctctat gaggagctaa
     1081 ctctcaggga acatttagaa acggtagcta tggcgtatga tattgccacc gatgaagtta
     1141 tggcacgtgc gcaaaaacta ttagagatgt ttcgtctaac agataaatta gactggtttc
     1201 caatgcattt ttcaaaggga atgaaacaaa aggttatgat tatttgtgct tttgttgtat
     1261 ctccaagttt atttattgtt gatgaaccat ttttaggttt ggatccacta gctatttcag
     1321 atttgattaa tcttctagca gaagaaaaag ctaaagggaa atctattttg atgtcaacac
     1381 acgtattaga ttctgccgaa aaaatgtgtg atcgttttgt tattttacat aaaggagaaa
     1441 tacgggcagt tggaacccta gaggaattac gggctatttt tggagacagt aatgctaatt
     1501 taaatgacat ctacatagct ctgacaaaag aggtgtgata tgaaaaagtt atttaataaa
     1561 cgtcgaagtc tctttttaac tcaaaatagt aaatatttga gatacgtctt taacgatcat
     1621 tttgtattag tattaatgtt tttgtcaggt tttttgttat atcagtatag ccagttacta
     1681 aaagactttc caaaaacaca ttggccaatt atcgtgattg tgtcaatcat tatcttaatg
     1741 ttactagcga tggggggaat agccagctat ttagaaccgg ctgataaaca atttttactt
     1801 ataaaagaag aagcaataaa ggaaattata aattctgcta aaaaacggac ttatatcttt
     1861 tggttggtta ttcaaacctt atttttagta ttaatatcac caattttgat aaagttaggc
     1921 ttgtcagttt ttatgattac attgttaatt tttggtctag gaataatcaa atggttggta
     1981 ataacttaca aggtaaaggt cttttataac aatcaaaatt tgaactggga tgcagctata
     2041 aatcatgagc aggaaagaaa acagagtata ttaaaatttt tctctttgtt tacaaatgtt
     2101 aaaggaattt cgacaagtgt aaaacgtcgt tcatttttag atggtatttt aaaacttatt
     2161 tctaaaacgc catcgcgttt atggactaac ttatttgtga gggcatttct aagaagctca
     2221 gattatcttg gtttaacaat aaggttggta actttaaata tcttatcagt catctttgtt
     2281 aacgaaacct acctcgccct tgctctggca tttgtattta actacttatt attgttccaa
     2341 ctattagctt taggtcacca ttttgattat caatacatga atcagctcta tccagttaga
     2401 ttaaatgcta aagcaagtca gctaaaaggt tttctcagag tattatcata tgctgtgaca
     2461 gtaattgata gcatacttat aagagaattg aaacctgtta tattattgat agttttgatg
     2521 ttaattgtga cggaatatta catcccttac aaaataaaga agatgattga ctaaagccta
     2581 taaaacaagt aaaataagag gaaacgacta aatgaaaagg agggactgct ggtgacaata
     2641 tcaaatcaag aattgacgct tactccctta agaggaaaaa gtggcaaagc ctatattggt
     2701 acttatccaa atggtgaacg tgtctttgtc aaatataata cgacacctat tttgccagcc
     2761 ttagctaagg aacagattgc gccacaacta ctgtgggcac gccgtacaag taatggcgat
     2821 atgatgagtg ctcaagaatg gttagatggt cgaacattga ctaaagaaga tatgggtagt
     2881 aaacagatta ttcatatttt attgcgcctg cacaagtctc gtcccttagt aaatcagctg
     2941 ttacaattag ggtataaaat tgaaaaccct tatgatttgt tgatggattg ggaaaaacaa
     3001 actcctattc aaatccggga aaacacttat ttgcaatcta ttgtgactga gttaaagcgt
     3061 agtttacctg agtttcgaac agaggttgca acaatcgttc acggagatat caaacatagc
     3121 aactgggtta ttacaacaag tggtttaatt tatttggttg attgggattc tgttcgttta
     3181 acagatcgca tgtatgacgt tgcatatatt ttaagtcatt atattccaca aaaacactgg
     3241 aaagattggc tgtcttacta cggttataaa gataatgaga aagtctggag caaaattatc
     3301 tggtatgggc aattttcata tttatcacag ataatcaaat gttttgataa acgagatatg
     3361 gagcatgtca atcaagaaat ttatgaatta cgaaagttta gagagttaat taaaaagcat
     3421 aatgcgagtt agaaaacgta agggtgcaga ggaacactta gaaaataatc ctcactatgt
     3481 tatttcaaat ccagaagaag ccaaagggcg ttggcatgag atttttggaa ataataaccc
     3541 cattcacata gaagttggtt ccggaaaagg ggcttttatc actggtatgg cagagcaaaa
     3601 tccagatatc aattatattg gaattgatat tcaattgtca gtgctttcct atgctttgga
     3661 taaagttcta gatagcggtg ctaagaatat caaattacta ttagttgatg gttctagtct
     3721 atcaaactac tttgatactg gcgaagttga tttgatgtat cttaacttta gtgatccatg
     3781 gcctaagaaa aagcatgaga aacgtcgatt gacctataag actttcttgg atacctataa
     3841 agacatactt cctgagcagg gggaaattca ttttaaaaca gataatagag gtttatttga
     3901 atatagcctt gctagctttt ctcaatacgg tatgacttta aaacaagttt ggttagattt
     3961 acatgcaagt gattaccagc aaaatatcat gactgagtac gaacgcaaat tttcaaataa
     4021 aggacaggtt atctatcgtg tagaagcacg attttagaat aagtaaatag taagtgggta
     4081 gttatttgct actcctttat ggagaggtcg catagtggct gagtgcgcac gcttggaaag
     4141 cgtgtagtcc ctaatggggg ctcgggggtt cgaatcccct cctctccttt aatcttgtta
     4201 atgtatcagt gtttattaag tgttcacctt tttcactcgt caaaagtcca agtttcgaaa
     4261 aatttctttt attttatcat cgtttctctt tgagcttgaa gtattagcat aaagttttct
     4321 aagctcttac aaagaactga ggacaaaatt ttaatttttt gatataagat tagtgtacgg
     4381 tgatgctaat ttaaagtaaa tgattgcaat aatcgtattt tgtgctataa tagtattaaa
     4441 taatacagaa aggggcggag ttttacttcg cctcttatgt gtgtgattca tagaatttat
     4501 agtcattata agttctaaag caagttagaa agaagaggta gctgttatcg caaatcaaac
     4561 tattgttgat attgttactc aagtcgtaac tcctgctatt caagcaccgt ttgaattagt
     4621 agatgtagaa tacgaaaaaa tgggtggcga ctatgtgctc agtattttaa ttgataaacc
     4681 cggaggcatt acggttgaag atacagcaca attgacagac gttgtaagtc ccttacttga
     4741 cacaattcaa ccagatccat ttccagaaca gtatatgctt gaagtctcaa gtcctgggtt
     4801 ggaaagacca cttaaaacag cagaagcatt atcaaatgct gttgggtctt atatcaatgt
     4861 aagtctgtat aaatccattg ataaagttaa aatatttgaa ggtgacttat tatcatttga
     4921 tggtgaaacc ttgacaattg attatatgga caaaacacgt cataaaactg tagatattcc
     4981 ctatcagacg gttgcaaaag cccgtcttgc ggttaaactt taagaaagga tgattcttgc
     5041 gaagcaagaa tggacactat gagcaaagaa atgctagaag ccttccgtat tttggaagaa
     5101 gaaaaacaca ttaataaaga agatattatt gatgcagtta ctgagtcact aaaatctgcc
     5161 tataagcgtc gttatggtca atcagaatca tgcgttattg aatttaacga gaagaaagca
     5221 gactttaccg tctatactgt tcgtgaagtt gttgatgaag tttttgatag ccgtttggaa
     5281 attagcctaa aagatgccct tgctattagc tcagcgtatg aacttggtga taagattcgc
     5341 tttgaggaat ctgttacaga atttggacgc gtggcagctc agtcagcaaa acaaactatt
     5401 atggaaaaaa tgcgtcgtca aatgcgtgaa gtaaccttca atgaatacaa acaacacgaa
     5461 ggtgaaatta tgactggtac tgttgaacga tttgaccaac gttttattta tgtcaatctc
     5521 ggttcacttg aggcgcaatt atcgcatcaa gatcagattc ctggagagag ctttaaatct
     5581 catgatatga ttgatgttta tgtctataaa gtggagaata atcctaaagg agtcaacgtt
     5641 tttgttagcc gtagtcatcc agagtttatc aaacgcatta tggagcgaga aattccagaa
     5701 gtatttgatg gaactgttga gattatgagc gtctctcgtg aggctggaga tcgtactaaa
     5761 gttgctgtac gcagtcataa ttcaaacgtt gatgcgattg gaacaattgt tggacgcggt
     5821 ggaagtaaca ttaaaaaagt tatcagtaat ttccatccta aacgcgttga tgctaaaaca
     5881 ggattggaga tcccggttga agaaaacatt gatgttattc aatgggttga agatccagcg
     5941 gaattcattt acaatgctat tgcaccagcc gaagttgata tggtactttt tgatgatgaa
     6001 gatacaaaac gtgctactgt agttgttcca gatagcaagt tatcgctagc tattggtcgc
     6061 cgaggacaaa atgtgcgttt agcagctcat ttgactggct atcgtattga tattaaatct
     6121 gcaagtgaat acgaaaaaat ggaagcgcaa gaacttcaaa ctgaggaagt agctcaagaa
     6181 tctgaagtta tttcagataa ggcagaataa tataaatgag agaggtgttt tatggctaaa
     6241 acaaaaaaaa tacctttaag aaaatcagtt gtttctggtg aagtgatcga taagcgagat
     6301 cttttacgaa ttgttaaaaa taaagaagga caggttttta tagatcctac cggaaagcaa
     6361 aatggtcgag gtgcctacat aaaattggac aatgatgaag ctatcctagc gaaaaagaaa
     6421 cgagtgtttg atcgcagttt ttcgatggaa gtttctgatg aattctatga tgaactattg
     6481 gcatatgtcg accataaggt caaaagaaga gagttaggcc ttgaataatt cagaaaaagt
     6541 attaaatctc attgggttag cacagcgtgc ggggcgcctt attacaggtg aagaattggt
     6601 tattaaagca attcaaaatc aacaagtatc gctaatcttt ctagcaaatg atgctggccc
     6661 aaatttgacg aaaaaagtaa ctgataaaag taattactac aaaacagaag tctccacagt
     6721 gttttcaaca ctggaattaa gtgatgcgtt aggcaaacca agaaaagtgg ttgctgtcgc
     6781 agacgctgga ttttcaaaga aaatgaggac tcttatggca taacagaata ggaggacatc
     6841 aattgtcaaa gaaaagatta cacgaaatcg ctaaggaaat tggaaaaacg agtaaggaag
     6901 tggttgagca agctcaatcg cttggcttac ctgtaaaaag tcatgcttct agcgttgaag
     6961 aaaatgatgc tacacgtatt gttgagagtt ttagctcttc taaaactaaa gctccaacaa
     7021 attctgttca aactaatcaa ggtgtgaaaa cagaatctaa aactgttgaa accaaacaag
     7081 gcttgtccga tgataaacct tcaactcaac ctgtagctaa accgaaacca caaagtcgta
     7141 actttaaggc ggagcgcgag gctcgtgcta aagctgaagc ggagaagcgt caacataatg
     7201 gggatcaccg taagaataat cgccataatg atacacgctc tgacgacaga cgccatcaag
     7261 gtcaaaagcg ttcaaatggt aatcgtaatg ataaccgaca aggtcagcaa aataaccgta
     7321 ataaaaatga tgggcgttat gctgatcata aacagaaacc ccaaacgcgt cctcagcaac
     7381 ctgcaggcaa tcgcattgac tttaaagctc gagcagcagc tttgaaagct gagcaaaatg
     7441 ctgaatactc acgtcatagt gagcagcgtt tccgtgaaga gcaagaagcc aaacgtcaag
     7501 cagctaagga gcaagaacta gctaaagcag cagcgttaaa agcgcaagaa gaagctcaaa
     7561 aagctaagga aaaacttgct tctaaacctg ttgctaaggt aaaagaaatt gtcaataaag
     7621 tagccgctac gccatcacag acagcagata gtcgtcgtaa aaaacaaact cgttctgata
     7681 agtctcgtca attttcaaat gaaaatgaag atggacaaaa gcaaacaaaa aataagaaaa
     7741 actggaataa tcaaaaccaa gtgagaaatc aaagaaatag taactggaat cataataaga
     7801 aaaataaaaa aggtaagact aatggagctc caaaaccagt aacagagcgt aaattccatg
     7861 aattaccaaa agaatttgaa tatactgagg gcatgaccgt tgcggaaatt gcaaaacgta
     7921 ttaaacgcga accagcggaa attgttaaaa aactctttat gatgggagtt atggcaacgc
     7981 agaatcaatc attagatgga gacactattg agctattgat ggttgactac gggattgaag
     8041 cacatgctaa ggttgaagtt gatgaagctg atattgaacg cttctttgca gatgaggatt
     8101 accttaatcc tgataactta acggaacgtc cgcctgttgt tactatcatg ggacacgttg
     8161 atcatggtaa aacaactctt cttgatactc tacgtaattc acgtgtcgct actggcgaag
     8221 ctggtggaat tactcaacat attggtgctt atcagattga agaagctggc aagaaaatta
     8281 ctttcttgga tacaccaggg catgcggcct ttacttctat gcgtgcgcgt ggtgcatcag
     8341 taactgatat tactatcctt atcgttgccg ctgatgatgg tgttatgccg cagacagttg
     8401 aggcaattaa tcactctaaa gctgcgggtg tcccaattat tgttgctatc aacaaaattg
     8461 ataaacctgg tgcaaatcca gaacgcgtta tcagtgaatt agctgagcat ggtgtcatct
     8521 ctactgcatg gggtggtgag tctgaatttg ttgaaatttc agctaagttt ggtaaaaata
     8581 ttcaagaatt acttgagaca gttcttttag ttgctgaaat ggaagaatta aaagcagatg
     8641 cagatgttcg tgctattggt actgttattg aagcccgtct tgataaaggt aagggagccg
     8701 ttgcaacgct tcttgtccaa caaggtactt taaatgtaca agatccaatc gttgttggta
     8761 atacatttgg acgtgttcgt gcgatgacta atgatcttgg gcgtcgtgtc aaagttgcgg
     8821 gtccatctac accagtttct attactggtt tgaacgaagc tccaatggct ggtgatcatt
     8881 ttgctgttta tgcagatgag aaagctgcgc gtgctgcagg tgaagaacgt gcaaaacgtg
     8941 ctctcttaaa acaacgccaa aacacgcaac gtgttagcct cgagaactta tttgataccc
     9001 ttaaagctgg tgaagttaag tcagttaatg ttattatcaa agctgatgtg caaggttctg
     9061 tagaagcact tgcagcttct ctccttaaga ttgatgttga aggagtaaaa gttaacgttg
     9121 ttcactcagc agttggtgct attaatgagt ctgatgtaac acttgctgaa gcatcaaatg
     9181 ctgttattat aggatttaac gtacgcccta ctccacaagc tcgtcaacaa gcagatgctg
     9241 atgatgtcga aattcgtcaa catagcatta tctataaagt tatcgaagaa gttgaagaag
     9301 cgatgaaagg taaacttgat cctgaatacc aagaaaaaat ccttggtgaa gccattattc
     9361 gtgagacctt taaagtttca aaagtgggaa ctattggtgg atttatggtt atcaatggta
     9421 aagttactcg tgattcaagt gttcgtgtta tccgtgatgg tgttgtcatt tttgatggta
     9481 aattagcaag tcttaaacac tataaagatg atgttaaaga agtcggcaat gctcaagaag
     9541 gtggtctaat gattgagaat tataacgatc tcaaagaaga tgacactatt gaagcataca
     9601 ttatggaaga aattaaacgt aaataacccc tatgagaaac tcaacttgta tctttagttg
     9661 gtagggaaat acaaaattat ggctaggctc gtcctagctt tcaagttatg agcacaatga
     9721 agaaaggatt aattatggct aatcatcgta ttgatcgcgt tggaatggaa attaaacgtg
     9781 aagtcaatga gattttgcgt ctccgagtta atgatccacg tgtacaagat gtcacaatta
     9841 ctgatgttca gatgttgggt gacctttcaa tggctaaagt attttatact attcattcga
     9901 cacttgcttc tgataatcaa aaagcacaaa ttggtttaga aaaagcaact ggtactatta
     9961 agcgtgaact tggcaaaaac cttactatgt ataaaatacc agatttacaa tttgttaaag
    10021 atgaatctat tgaatacgga aataagattg atgaaatgtt gcgcaatcta gataaaaaag
    10081 attagaatca agattaagaa taataaaaaa taaaccatgt caaaatcatg gtttattttt
    10141 tatagcttta gagttgagat agctagtatt aaggagtttt attgatgata tcaatcattt
    10201 gatactgtgc tgttttagct tcttcaatag ggtaaattgg ataaacatga ttcattccct
    10261 cttgtgcaat atagttaacg gggtgattag ctgcttggag ttgtgcagca taatcgcgaa
    10321 tatctgggaa gaatatttct cgtgttccag taaataacgt aataggagcg agtttagtag
    10381 caggggcatt tttaggacta acatatgtat ggttagtatt attagaacca tttgcccaaa
    10441 tttcacctac acgtgcgaga ccccaagcag ataggatagg atcagtatct tcgtatttgg
    10501 gaatttctgg atgtttcatt gtcacatcta gccatggaga taagaggata atatttttag
    10561 gttgaggaat agcctcttgc cctgattggt gagacaatgc atgcgctaat cctagtgcta
    10621 atcctccgcc agctgaatca cccatcaaag tgagattagc gtttttttca tgaaagtgtc
    10681 tataaaggtt aaccaatcta gggatagcat aatcataggt ataacgagga gtttttggat
    10741 aaattggtaa aataattttt gcatcaagac tagttgagag cgtcttaagc atgctgatat
    10801 ggtaaggcgt tggattattt aagtaagagc caccagctaa atagaaaatg gtcttttgat
    10861 tagctttacc gttgtcattc caagaataga cttgcatatc aaaaagtctc tcttgaacaa
    10921 gccctccatt aacagagttt gggtctaagt aataaccctt atcagattgt tcacggctag
    10981 catttaagta cttttgaaat gagctttcat cttgaacttt ataataactt ttatcgcgct
    11041 ttagccaaaa gtattgttcg ataaaccaac ttttccatga acgcccactt gcttttgcat
    11101 gtggtgtaca ggcgatggat aaaatggtga taatactaaa tagtagcaat agttttttga
    11161 aggacggttt caaggtattc tccttaatat aatgttattt tttgtgtaat attattataa
    11221 taaaaccaac atctttgtca atagaataaa gaaagatgag aaatgttttt cggtttacaa
    11281 atgtagattt atttgctaaa attggtttac aaacgtaaat cgaaagaggt gagaaaatga
    11341 ctatttcaag cgcagaatgg gaaattatga gagtggtttg ggcacagcag aatacaacca
    11401 gtaatgagat actagcagtt ttactagaga agtatgattg gactccttca actgtcaaaa
    11461 ccttattaag aaggctctta gataaaggct acgtgagcag agaaaaaatg ggaaaaggat
    11521 ttagctattc tcctttaatt gatgaagatt tagctatgat gtcagaggta gatagtgtat
    11581 ttcaaaaagt ttgccagacc aaacatgttg ctattgttag acacttattg gaaagtatac
    11641 caatgacaga gaaggatcga ttaaatttgc aatcatcact agaagctaag aaaggcaaaa
    11701 cacttgaacg tgtagcttgt aattgcattc ctggtcagtg tcaatgccat tagaaaggag
    11761 aaaatatggc taatagaaaa gaaacatttt tgattgatgg gatgacttgt gcatcttgtg
    11821 ctttaacgat agaaaaagct gtcaacaagt tagaccatgt ggatagtgca gtagtaaatt
    11881 tagcaacaga gaaaatgaca gttacgtttg atgatacaac cttatcgcca aatgtgattg
    11941 aagagtgtgt ttctgagagt ggatacgaag catcgctttt caaagaggaa acttctaaaa
    12001 gccaatctga acggcaccaa ttagccattg aaaagatgtg gcatcgcttt tggatgtcag
    12061 cagttgcaac tatacctctt ctctatattt ccatgggacc aatgattaac ttatggttac
    12121 catcattttt gatgccagat aagggacctc taaactatgg tatgatacaa ttattgttaa
    12181 cactaccggt gatgtatttt gggcgtatct tttaccaaaa tggttttaaa gcacttttta
    12241 aaaggcatcc caatatggat tcattagttg ctatagccac aacagcggct ttcatttata
    12301 gcttatatgg attgtacgaa atattacagg gtgacataca ctacgctcac cagctttatt
    12361 tcgaatctgt tgcagttatt ttaacactta ttactctagg aaaatatttt gaaatattat
    12421 ccaaagggcg tacttcggcc tcgattgaaa aattattaac tttatcagca aaagaagcaa
    12481 gagttatcaa agatggtgaa gactatatgg ttcctttgga taaagttaaa ataggtgaaa
    12541 caattcttgt caaacctgga gaaaaaattc ctcttgatgg ccatgttgta gcaggagaat
    12601 caagtattga tgaatccatg ttaacaggtg aaagtattcc agttgaaaaa aaggttggta
    12661 gtaaggttta cggtgccagc attaatgggc aaggtagctt aacgattttc gttgaaaaag
    12721 aagctggcgg ttcccttcta tcacaaatta ttaacttagt agaagctgca cagacaagta
    12781 aggctccaat tgctaactta gcagacaaag tatcaggagt ctttgtccct tttgttattg
    12841 tcattgcaat tttatcaggc ttgagttggt acttaattct ggggcaaagt tttgccttct
    12901 ctctgaagat tatgattgca gtcttagtga ttgcttgtcc atgtgcttta gggttggcaa
    12961 ctcctacagc tattatggtg gctagtggta aagcagctga gaatggtatt ttatttaaag
    13021 gtggtgaagt gttggaaaag gctcaccaca ttgatacaat agtttttgat aaaacaggaa
    13081 ccttgaccaa agggaaacca gaagtagttg ctatcaaaac atatggggga gataaagagg
    13141 agtttttagg tcaggtagca tcagtagaaa aactttcaaa tcatccactt agccaaacaa
    13201 ttgttaataa agcaaaagag aaagaacttc ccttaaggga agtcatggct tttaaaaata
    13261 ttttgggtta cggtttgagt gcaactatta atggtaaaac tatgcttgta ggtaatgcta
    13321 acttaatgac taagaacgat gttaatttag atttagcaaa ggctgatata gaaatagctc
    13381 aagaagaagc acaaactgtg gtttacgttt ccgaaaatgg agtgttgagc ggtttaataa
    13441 ctttaacgga ccagctaaag acagatagcc aagaaacagt taagcagtta caaagattag
    13501 gatttaattt agttttactt actggtgata ataaggctag tgcagatgcc atagcacaaa
    13561 aattgggtat cacgacagtt gtttcagaag ttttacctga tcagaaagca aacgttattt
    13621 tagaacttaa agaaaaaggc ggacagattg cgatggttgg tgatggcatc aatgatgctc
    13681 ctgcgctagc tagcagtgat gtaggtatct ctatgagttc tggaacagat attgctattg
    13741 aatcagcaga tatcgtgttg atgaaacctg agttaacaga tctcttaaaa gcaatgacta
    13801 tcagtaaaca aacgattcaa attattaagg aaaatttatt ttgggctttc ttttataacg
    13861 ttttagcgat tccagtggca atgggtgttt tacatctttt tggtggtccc ttactcaatc
    13921 ctatgttggc aggcttagct atggcgttta gttcagtttc agtagtttta aacgcacttc
    13981 gcttaaaagt cttaaaataa taaagataat aagactgaat ttttatgaag ggagaagaat
    14041 atgaaacata cctatcgagt tagtggcatg aaatgtgatg gttgtgccaa aacagtttct
    14101 gataagttat caagtgtgat tggtgttgac gaggttaatg ttgatttgac taaaaatcaa
    14161 gttgtcgtaa gtggaaaaac attcaaatgg cttttaaaac gttctttaaa agacacaaag
    14221 tattcactag aagaggaaat aaattaataa ctaaagaagt ggttacagct tttaatttct
    14281 taatttgagc agtttctcac ttctttttta ttctaaaaat gataaactag aatagaatta
    14341 gttgtgagga gtgcagatgt caattgatat ttggaacatt ttaagtttgg ttggaaccgt
    14401 agcttttgca agttcgggag ctattgtggc gattgaggaa gaatttgata ttctagggct
    14461 ttttatctta ggttttgtaa cggcatttgg tggtggagct attagaaatg tactaattgg
    14521 cttgcctatt gaaacacttt ggagtcaagg aattgctttt tatgcagcag cagcagctat
    14581 tttatttatt atgattttcc ctaatttgtt gagtggtaag ggacgggatg ccgaagttgt
    14641 atcagatgct attgggttag cagcgtttag tgttcaagga gcactatatg cgactcaatc
    14701 tcatcaacct cttagtgctg tcattgtcgc agcagtacta actggtgcag gtggtggtat
    14761 tgtcagagat gttctggctg gccgaaaacc tggagtacta agaagtgaaa tatatgcagg
    14821 ttggtcaata ttagtgggga tcattttata ttttaaaatt gctaaaacaa caactgacta
    14881 ttatttgtta gtgttagttg taactagcct aaggatgttg ggttataaga aacaatggca
    14941 cttgccagtt gtcagaccac gtaagaaatg atagaggaga aaaagatgat aaaattaatt
    15001 gctattgata tggatggaac gcttttaaat gatgaaaaga aaattccaaa ggaaaatatc
    15061 caagctatta aagaagcaac tcaagcaggt attaaaattg tactgtgtac tgggcgaccg
    15121 atgtcaggta tacttcctta ttttaatgaa ctaggtttaa caaaagagga gtatattatc
    15181 atgaataatg gttgtagcac ttatagcacg aaagattggc aattgattga ttctgcaact
    15241 ttaacacatg atgaactcat ctttcttgag gaggttgtta aagaatttcc caacgtttgt
    15301 ttaacattaa cagctgaaaa tacattttac gctgttggag aggaagttcc tgaaattgtt
    15361 gcttatgatg ctgatttagt ttttacaaaa gcaaagtcaa caagtttaga tgctcttcgc
    15421 aatcaagaag aaattgtctt tcaagcaatg tacatgggat tagatgcaga tgtaactgct
    15481 tttcaagaag ctgttgaaga agccttaatc tcgaaattta gtggagttcg aagccaagac
    15541 tatatttatg agattatgcc tcaaggagta acaaaagcaa gaggcttaaa atccttgata
    15601 gcaaaattag gactggatat taatcaagtt atggccatcg gtgatgctcc taacgatatt
    15661 gaattattag atttagttcc caatagcgtt gctatgggga atgcatcaga tgagattaaa
    15721 tctagatgta aatatataac ggtggataat aacaaagcag gggttgctaa agctatttat
    15781 gactatgctt tacgttaaaa tagatataga ataataaagg gaactttaaa cactgtttgt
    15841 ttagggttct tttgtttaat aaactaacaa aattcagaag tttacagata gcttatgcta
    15901 taatagacct atgacaaaca agaataaatt acttttaatt gatggctcat ctgtagcctt
    15961 tcgtgctttt tttgctcttt ataatcaaat tgaccgcttt aaaaacaatt ctggacttca
    16021 tactaatgcc atatatggtt tccatttgat gttgaatcat attttaggtc gtgtccagcc
    16081 tagtcatatt ttagtggctt ttgatgccgg aaaaactact ttccgtacgg aaatgtatgc
    16141 agattataaa ggtgggcgtg ctaaaactcc ggatgaattt cgtgaacaat ttccttacat
    16201 tcgtcaacaa ttagatgtct taggcatcaa acattatgaa ttggaacatt atgaagccga
    16261 tgatatcatt ggtactcttg caaaacaagc cgaagctagt aatgaacatt ttgatataac
    16321 ggtggtttct ggggataaag atttaattca attaacagac actaacacgg tagtagaaat
    16381 ctctaaaaag ggagttgctg agtttgaaga attcacgcca gcttacctta tggaaaaaat
    16441 gggaattacg ccgagtcaat ttattgattt aaaagctttg atgggagata aatcagataa
    16501 tattcctggg gttactaaaa tcggtgaaaa aactggcttg aagttgctat cagaatatgg
    16561 ttccttagaa gggatctatg aaaatattga ggctatgaaa cagtctaaga tgaaagaaaa
    16621 ccttatcaat gataaggagc aagctttctt atcaaagacc ctagctacaa tcaacatagc
    16681 atcacctata acaatcggct tagaagatat cctttatagt ggaccgcaag atataaaagc
    16741 cttgtctcaa ttttatgatg agatggattt taaacagttt aaagcagctc tgggtgagga
    16801 gacaagtcag gaagattttg aggttgactt tactgaagtg gagcaattaa aaacagaaat
    16861 gttctccgat aatgattttt attactttga gatgttgggt gataactacc atgtagaaga
    16921 tttaattggg atagcttggg gaaatagcga tacaatctac gctactagta atgtatcgtt
    16981 attgcaagaa gctttattta aaaaagcttt atcaaaacct atcaaaactt atgattttaa
    17041 aagaagtaag gtccttttaa atcgttttaa tattgattta ccagagccag cctttgatac
    17101 tagattggcc aagtaccttt tgtctactac tgaggataat ctagtatcaa caattgctag
    17161 gttatataca aatctgccac tggatactga tgatgctgtt tatgggaaag gtgctaaacg
    17221 cgctatacca gaaaaaacac gatttcttga gcatctagct aaaaaagtaa aagttttagt
    17281 ggattctgag gctaatatta tgcaacagct taaagctaat gagcaagaag aattgctgtt
    17341 tgaaatggag caacctttag cgaatgtttt agctaaaatg gaaattcgag gtattaaggt
    17401 taagaaaaat accttgaatg aaatggccat tgaaaatcaa aaagtcatcg aaacattaac
    17461 acaagaaatc tatgaattgg ctgggcaaga gtttaatatt aattctccaa aacagttagg
    17521 aaagttgttg tttgaaacgt taggtcttcc tgtagaaatg actaaaaaaa caaaaacagg
    17581 ttattcaact gcagtagatg tattagagcg cttagctccg atttctcctc ttgtaactaa
    17641 aattttagag taccgtcaaa tcactaaatt gcaatcaacc tatattattg gtttacaaga
    17701 ttatatttta gaagatggta aaattcatac acgttatgtt caagatttga ctcagacagg
    17761 tcgtctttca agttctgacc caaaccttca aaatatacca gttagactag agcaaggacg
    17821 gcttatccga aaagcttttg ttccatcaga agacaatgct gttttattga gttcagatta
    17881 ttctcaaata gaattgcgcg tgttagcaca tatttcaaaa gacgaacatt tgatcgctgc
    17941 ctttaaagag ggagctgata ttcatacttc aacagctatg cgtgtttttg gtatcgaaaa
    18001 acctgagaat gtcactccca atgatcgtcg aaatgctaag gctgttaatt ttggaattgt
    18061 ttatggtatt tcagattttg gtctatcgca taatttagga atacctcgta aactagcgaa
    18121 gcaatatatt gacacctatt ttgagcgtta tcctggaata aaaaactata tggaaacagt
    18181 tgttcgtgaa gcaaaagata agggttacgt tgaaacactt ttccatcgtc gtcgttcatt
    18241 accagatatt aattctcgta attttaacat tcgccaattt gcagagcgca cagcaatcaa
    18301 ttcaccaatc caaggaagtg cagctgatat tcttaaaatt gcaatgataa atttagatag
    18361 ggtacttgat aaaggtggtt ataaatcaaa aatgctgttg caagttcatg atgaaattgt
    18421 acttgaagtc cctaacgagg agataggagc aatcagggag ttggttacaa aaacaatgga
    18481 gtcagcaatt agtttatcgg tacctttgat tgcagatgag aacgcaggtg agacttggta
    18541 tgaagcgaag tagtggacaa catacgtaaa ggagtcatta atatggttta tcattttcaa
    18601 aatccaagtg attttatgtt aaaaaattat ttaacaaaag caaagaccat cgcagttgta
    18661 ggactgtcag accgtcaaga aactgcagct tatcaagtat caaaaataat gcaagaagca
    18721 ggttatcaaa ttattccagt taatcctaaa aatgcaggac agaaaatact tgggcaaatg
    18781 acatatgcct cacttaagga tgtcactgaa catattgata ttgttaatat ttttcgacgt
    18841 agcgagtatt tacctgatat tgcaagagaa tttttagaag tagacgctga tattttctgg
    18901 gctcagcttg gacttgaaag tcaagaagca gagacaattc taaagcaagc aggacacaaa
    18961 cagattgtca tgaataaatg tctgaaagtt gaatgccaaa aattacactc agaaactaac
    19021 taattaccgt gtttaatagg caaataatat aaattatgct ataataacca tgctaaatta
    19081 aagttcgtgc tggaaaggag tcctatggac agtcattcac atggtcatag acctctcgat
    19141 gcttatgaaa acgtcctaga gcatctccgt gaaaaacgta ttcgaattac tgagactcga
    19201 aaagctatta tttcctatat ggttaatagt cgtgagcatc cgagtgcaga aaagatttac
    19261 aatgatttat taccagaata tcctaacatg agcttggcaa cagtttacaa taaccttaag
    19321 gtccttgtgg atgaggggtt tgtgacagag ctaaaacttt gcaattatag tacaacttat
    19381 tatgatttta tggggcatca gcacctaaac attgcctgtg aagattgtgg gaaaatcgtt
    19441 gattttgtcg atgttgactt attggatatt tctagagaag cacaccaaca aacaggtttt
    19501 gaagtgacta gagttcagtt agtagcttat ggtatctgtc cggagtgtca gaggaagcgt
    19561 aaggataaat ataaaaaaga ttgaaacgta attgttcaat ctttttttgt ttatagacta
    19621 agagaaaaag tttaagtttt actaagtaat aattaagaaa taatttataa ttgcttaaaa
    19681 aatgatttat tttttgttgg tatactctca ttaacaaaaa gaaagaggaa tcccttatga
    19741 aaatatcaca atacaataaa tggtcaattc gccgattgaa agttggagct gcatctgtaa
    19801 tgattgctag tggaagcatt gttgctttag ggcaatctca tattgtttca gcagatgaga
    19861 tgtcccaacc taaaacaacc attacagctc caacagctaa cacttctaca aatgtagaaa
    19921 gtagtactga taaggcatta tcaaaagtta ctacgatgga gacaagttca gaaatgccta
    19981 aaatgcaaaa tatggcaaaa gttgaaaaaa cgagtgacaa acctatgatg gtggctacct
    20041 ctgttagaaa aatgatggca acgccgacac ctgtagcgat gaccaaaacg actagtgtag
    20101 atgaggtaaa gaaatcaact gatactgctt ttaaacagac agtagacgtt ccggctcatt
    20161 atgttaatgc agcaaaaggg aatggcccat ttctagcagg tgtaaatcaa acgattccat
    20221 atgaagcgtt tggtggagat ggcatgttaa cgcgtttaat ccttaaatct tctgaaggag
    20281 caaaatggtc agataatggt gttgataaaa atagtccatt attaccattg aaaggcttaa
    20341 ctaaaggtaa atatttctat caagtttctt taaatggtaa tacgacaggt aaagagggac
    20401 aagctctctt agatcaaatt aaagctaatg ataaacatag ctaccaagcc acaattagag
    20461 tttatggtgc taaagatggt aaagttgatt taaaaaacat gattagccaa aaaatggtaa
    20521 ccattaacat tcctcacatt acaactgata tggaagtaaa aaattctcta aagatggcct
    20581 ttaaagaaaa agtagacgtc ccagcaaaat atgttagtgc tgcaaaagct aaaggtccat
    20641 ttttagcagg tgttaacgaa acgattccat atgaagcgtt tggtggggat ggcatgttaa
    20701 cgcgtttaat ccttaaagct tcagagggag caaaatggtc agacaatggt gttgataaaa
    20761 atagtccatt attaccgtta aaagatttaa ctaagggtaa atacttctac caagtctctc
    20821 ttaatggcaa tacagccggt aaaaaaggcc aagctctctt agatcaaatt aaggcaaatg
    20881 gaagccatac ttatcaagca actatcacta tttatggtac gaaagatggc aaagttgata
    20941 tgaatactat tttaggacaa aagacagtga tgattcatat taatgtcgct aaaaaagata
    21001 tgaatagcac atcaatgatg atgaaaaaag ataagatgac aatgcctatg aagaaagaaa
    21061 tgacttcatc taaaataaat accggtatga tgatgtctaa caacaagatg tcagctaata
    21121 tgcaaatgtc atcacaagcc aaatcaaatg ataaagctgg taaaaaaatg tctatgatgt
    21181 ctaaaaattt acctaataca ggtgaaacaa agcaacaaaa tgtaggtgtg ctaggtatgc
    21241 ttagtctagc ctttgcgaca ggtctaactg cgctaggcct taaaaaatct aaacaaagat
    21301 agggatagta tttaattcag aacaccaaat ttgtttaaaa ttattgaggg ttatcgtggt
    21361 aaaatagtgt tatactagag gtatagcact attttgactc attaggagag tagaatgaca
    21421 caaaaattat tattagttga cgatgaattt gaaattattg atattaatag acgttatcta
    21481 gagcaagcag gatatgaagt gtcagtagct gcggatggca tagaagcact caaagaggta
    21541 gatgagaatc gttttgacct aattattagt gatattatga tgcctaaaat ggatggctat
    21601 gattttatta gtgaagttct agttcgagaa cccaaccaac cttttctctt tataaccgct
    21661 aaagtatctg aaccagataa aatttactct ttgagtatgg gtgcagatga ctttatttcc
    21721 aagcctttca gtcctaggga attagtttta cgtgttaaaa atattctaag acgtatctat
    21781 gggaatcatc aacaatctga agtgttgacg attggtgatt tggttatcga ccaaaaacaa
    21841 cgtttggtaa tggtggactg caatacaatt tccttaacca ataaatcatt tgacttgctt
    21901 tggatattag caaatcattt gaatcgtgtt ttttcaaaaa cagagcttta tgaacgtgtc
    21961 tggggcgaag aatttttaga tgacactaat actttgaatg ttcatatcca tgccttacgc
    22021 aatgaccttg ctaaatttag tacagacaat accccaacta tcaaaacagt ttggggatta
    22081 gggtataaat tagaggaata aaatgaaatt aaaatattat attgttattg gttaccttat
    22141 ttctatgtta ataactgtag caggtgtttt ctttgggctt aatcatatgc tgattgagac
    22201 tcgaggtgtt tactacatcc tttcagttac aattattgcc tgtattgtag gtggtatagt
    22261 taatttgttc ttattatcaa gtgtatttac atctttgaaa aagttaaaac aaaaaatgaa
    22321 ggatatttct caaagatgtt ttgatacaaa agcacaaatt tgttcaccac aagaattcaa
    22381 ggatttagag acagccttta accaaatgtc ttcggagtta gagtcaacgt ttaaatctct
    22441 aaacgagagt gaacgcgaaa aaacaatgat gattgcacaa ctttcacatg atatcaagac
    22501 tccaattact tctatacaat caacagtaga aggtatttta gatggtatta tatctgaaga
    22561 agaagttaac tattatctca ataccatttc tcgtcaaaca aaccgtctta accatctggt
    22621 tgaagaatta agttttatta cattagagac tatgtcagac acggctgaac ctcataaaga
    22681 agaaacaatt tatctggata aactactgat tgatatttta tctgaatttc aattggtgtt
    22741 tgaaaaagaa aatcgccaag taatgattga tgttgcgcca gatgtttcga aattatctag
    22801 tcaatatgat aaattatcac gaattttatt gaatttgatt agcaatgcag tcaaatattc
    22861 tgatcctgga agtcccttga ctatcaaggc ctattcaaat cgtcaagata tcgttatcga
    22921 tattattgac caaggctatg ggataaaaga tgaagatctt gcttctattt ttaatcgtct
    22981 ttatcgtgta gaatcttcac gaaatatgaa gacaggagga catggtctag gtctttatat
    23041 tgcgcgacaa cttgcacatc aactgaatgg ggatattttg gtcgaaagtc agtatcaaaa
    23101 aggcagtaag ttctctttag tacttaaatt acaaaaatag ctaggaatta ttcctagcta
    23161 ttttttgtaa tgtttctata aaagattaag cgcgcagtag tttggtaagg aaggagatag
    23221 atatagacta atccgaaact gaagattgta aggaactgcc aaccaataaa acttaagtct
    23281 aaaaggaaga gtctaagttt attaccattc attagttctc gactagcaac cagtacttta
    23341 gacggtccct catagtttcc actttctaat tgagtgtaaa atactaaagt tgagatacta
    23401 taggagtaat attgttgaac gagttttata actgccttag tagtaattag gctactaaca
    23461 actattaaaa caggggcaag gataaggtta ccactcaaaa gtagcctaaa acttgaaatg
    23521 atgattagta gaccaaaagt acttagtaga taaaagaaag ataaaattag tccttttaga
    23581 acaagtactg agagtaattt ccagaaaata tggcttgaaa atacataagt attgtccgag
    23641 aatctaacgt ttagtttctg atttttgacg acatctaaca ttacaaaact gactgaaata
    23701 ataaaaagtg tgtaaaagaa ataaactgga agaggtacaa aaaaatcaag tagtgtcata
    23761 ttattatatt gataataaaa tgatatatat aacgtaataa tactaaggag aatcgggagt
    23821 aaaaaaagtt gattcttacc ttgaagattg cttaatatag tttttgcctc atttttaatt
    23881 tctgaattcg tcatatctcc tccatacaca aaagtacaat tcatttcatt ataacacatt
    23941 ccttaagatg agcatgacaa gagtacattt ttttgataaa atcttaatgg ctaaaaaaga
    24001 aattaataca aagtattaca tttagatatt gattagcact aaagcccatg actgtttttc
    24061 atggaagcaa ggagaaaacg atgactgatc atccaataaa atatcgatta attaaacagg
    24121 aaaaacacac aggagccaga cttggtgaaa taatcacacc acatggtaca tttccgacac
    24181 caatgtttat gcctgttggg acacaagcaa ctgttaagac acaatctcct gaagaactaa
    24241 aagaaatggg ttcaggaatt attctatcaa atacctacca tttatggtta cgtccaggtg
    24301 atgaattgat tgctaaagct ggaggtttac ataaattcat gaactgggat caggctattc
    24361 taacagacag tggaggcttt caggtttatt ctttggctga tagtcgaaac atcactgaag
    24421 aaggagtaac ctttaaaaac catttaaatg gtgcgaaaat gtttttatca ccagaaaaag
    24481 ctatttcaat tcaaaataac cttggttctg atattatgat gagttttgat gagtgtccac
    24541 aattttacca gccttatgat tatgtaaaaa aatcaattga gcgaacaagc cgttgggcag
    24601 aacgtggatt aaatgcccac cgccgtcctc atgatcaagg actatttggt attgttcaag
    24661 gggcagggtt tgaggattta cgtcgtcaat cagcacgaga tttagtatcg atggattttc
    24721 caggttattc aattggaggg cttgcggtcg gagaaacaca tgatgagatg aatgcagttc
    24781 ttgattttac tgttccaatg cttccgaatg ataagcctcg ttatttgatg ggagttggtg
    24841 caccagatag tttaatagac gccgttatcc gaggagtaga tatgtttgat tgcgtcttac
    24901 ctactcgtat tgctcgaaat gggacatgta tgacaagtca aggtcgctta gttgttaaga
    24961 atgctaaatt tgcagaagat tttacgcctc ttgatccaaa ttgcgattgc tatacttgta
    25021 aaaattatac acgcgcttat attcgtcact tattgaaagc agatgaaact tttggtattc
    25081 gtttgactag ttaccataat ctttatttcc ttgtcaatct tatgaaagat gtcagacaag
    25141 ctattatgga tgataatctt ctagaattcc gtcaagattt tatggagcgc tatggctatg
    25201 gcatgaataa ccgtaatttt taaaaaagtt cctggtaaaa cagggctttt ttttgaatta
    25261 tttgttaacc gttttttaaa aaaagttgac aatagaaaaa cttttgtata atatgttata
    25321 ggaggaatca tgatgcaaga gtactttggt attggtcttg atagtagcag ccgctgttac
    25381 cactatcata ctaaattgga tattgtcgct ttaaaatgtg ctgtttgtca aaaatattat
    25441 gcttgttata aatgtcatga tgcactcgag gagcattgtt ttgcagcaac aaaatctgat
    25501 gaaacgtttc ctgtcttatg cggttcttgt cgtcagatgt tgacattaaa agagtataaa
    25561 actggttttt gtccttattg tcgtatgctt tttaatccaa attgtcaacg ccatgattct
    25621 atttattttt cgaaggagta atttttaatg tttcacacaa aaagcctaat ccagatggct
    25681 atgatggcta ctttattggt ggtacttggt ttcattcctg ctctaccact tggtttcatt
    25741 ccggtcccta tcgtcttaca aaacctaggg gttatgatgg ctggtgttat tttgggcgga
    25801 agaaaaggga gtttatctat tttgctcttc tttcttgtcg gtttagttat tcctgtattc
    25861 tcaggtttac gttcgactat acctgtattg acaggaccaa cagctggtta tgttattgct
    25921 tggttgtttg ttcctttgct tatctcatat ggtgtaaaat tagtaaataa aaagaatttt
    25981 atagctattt tccttattgt atggctagca ggggtccttt ttgtagatgt agcaggagca
    26041 gtttggctgg catcttatac gaacatccca ttagataaat cacttcttca aaacttagta
    26101 tttatcccag gagatacaat aaaagccatt atagctgctg ttgttgctgt taaatataaa
    26161 gatagttttt tcttgactga aaaataatca agattgctat atattagatg gactagtaaa
    26221 tctaaaaatt tagatttact agtctttttt gtgcataaaa tctaaaacag ttattcttaa
    26281 taaaatttgg tataatagca ttaaatcagt aaggagggga ttatcatgaa attaacaacg
    26341 ttaggttgtt ggggtggtta tccttataaa gatggaggaa ccacaagttt tctattaact
    26401 ggagaaaata attttcactt attaatagat gcgggaagtc gttcaatcaa tgagctagaa
    26461 aaagaaataa gcccgttgga tttggatgca gttatcatca gtcattatca tccagatcat
    26521 gtagcagatt tgggtgtgtt gtatcattat cgtttgctat accctaaaga taaatgggaa
    26581 cccaaagtat tgccagttta tggacataat gaggatgatt atgagttttc taaattgaca
    26641 ttacctaacg ttgctcaagg aattgcttat cataccaatg gagttgagca agttggtccc
    26701 ttcaatatta cgtttatcaa aacagtccac ccagtcgtct gctatgccat gaggattaca
    26761 gaaagagaaa ctggtcaagt attagtcttt acaggagatt ctgggtattt tgctgacctt
    26821 gtacctttta caaaaggcgc agatttattt ttagcagacg tttatcttta cgagggaaat
    26881 gaaaatcata ttgcacattt gactagtaaa gaagcaggtg agattgctta taacgctaaa
    26941 gttaagaaat tagttttaac acatgttccg cagtttccgg gagaaggaat aaaccccgaa
    27001 aatcatttgg aagaacttcg actacagtct gaaaagtatg caggtggcat ctctgttgat
    27061 ttagctcagc cacataaatc ttgggactta ggaaaactat aatgcagaaa tttacccaag
    27121 aagaaaaaga atggtttatg acagaagctc taaaggaggc aaaatcctcc ttagagaaag
    27181 aagaaattcc tataggttgt gtcattgtaa aagatggtca tattattgga agaggacata
    27241 atgctcgtga agagtttaat aaagctattt tacatgctga aatcatggct attaacaatg
    27301 caaatgagaa ggtagggaat tggcgcttat tagatactac tttatttgtt actgtagaac
    27361 cttgtgtaat gtgtagtgga gctattggct tagctagaat tccccacgtt atatatggcg
    27421 ctaagaatac gaaatttgga gcagcgggaa gtctctatga tattttaagt gatagccgac
    27481 ttaatcatag agttgaggtt gaaaccggca ttttagaaga tcaatgcgct aaaataatgc
    27541 aggatttctt tcgaaaagga cgacaacgca agaaagaagc taaactaagt gaaaaaacta
    27601 agatttgata gcttgcaata aaatattagt tttgttataa tatctttgga gcaacattta
    27661 ctcgtgaagt gggtcagggg aggaatccag cagccctaag cgatgctaaa tgtgtgctct
    27721 ttttattttc aaaagtcaaa gtgtttttgg aaataattat gaaagtttca taaaaattgg
    27781 tagcgtttca aaaaatatga aaatacagtt aaaaaacctt gaaaaaaaac tcacaaaaga
    27841 gtaagatgat agtgaaacat aattgttagg agaaataaaa tgacacatat tacatttgac
    27901 tattcaaaag tcttgggtca atttgtaggc gaacacgagt tagactacct acaaccacaa
    27961 gtaagtgcag cagatgcttt ccttcgtcaa gggactggtc ctggctcaga ttttctcgga
    28021 tggatggacc tcccagaaaa ctatgacaaa gaagaatttt ctcgcattca aaaagccgct
    28081 gaaaagatta aatcagatag cgaagtactc gtggttattg gtattggtgg ttcgtacctt
    28141 ggcgcaaaag cagcaattga ctttttgaat aatcattttg ctaatttgca aaccgcagaa
    28201 gaacgtaaag cgcctcagat tctttatgct ggaaattcta tttcatctac ttaccttgcc
    28261 gatttagttg aatacgtcca agataaagaa ttctcagtaa atgtcatttc aaaatcaggt
    28321 acaacaactg aaccagcgat tgctttccgt gtatttaaag aacttctagt taaaaagtac
    28381 ggtcaagaag aagctaataa acgtatctac gctacaactg ataaagttaa aggtgccgta
    28441 aaagttgaag cagatgctaa caattgggaa acatttgtag ttcctgataa tgttggtggt
    28501 cgtttctcag tactgacagc cgtaggtctt cttccaattg ctgcttcagg agcagatatt
    28561 actgctctta tggaaggcgc aaatgctgct cgtaaagatt tatcttcaga taaaatctct
    28621 gaaaacattg cttatcagta cgctgcagtt cgtaatgttc tttatcgcaa aggttatatc
    28681 acagagattt tagctaacta cgaaccatca ttacaatact ttggtgaatg gtggaaacaa
    28741 ttagcaggtg aatcagaagg taaagatcaa aaaggtatct atcctacatc agcaaacttc
    28801 tcaactgatc ttcactcact tggtcaattt attcaagaag gttaccgtaa cctctttgag
    28861 acagttgttc gcgttgagaa acctcgtaaa aatgtgacta ttcctgaatt aaccgaagat
    28921 ttagatggtc ttggttatct tcaaggaaaa gatgtagatt ttgttaataa aaaagcaaca
    28981 gatggtgtac tccttgctca tacagatggt ggggttccaa atatgtttgt aacgcttcct
    29041 acacaagacg cttacaccct tggttacact atttacttct ttgagttagc aattggcctt
    29101 tcaggttatc ttaactcagt aaatccattt gatcaaccag gggtagaagc atataaacgt
    29161 aatatgtttg ctcttctagg taaacctgga ttcgaagagc ttagcgctga attgaatgca
    29221 cgtctttaaa aatatctaac taaaaagttc ctcaaagaga ggaacttttt agttttgttg
    29281 taggataact aaaaacccac tagcaccggt agcgatagtg ggttggaaaa agggttgaga
    29341 aaagttatta acttttctca ggagatttat gaaaaaggaa aataatttag gataatattt
    29401 tcctaacggc taggataaac cgcttatttt aggatgatac tagtatacag tagaaacctt
    29461 aaataaacct taaaaattca tactttatca ttttattttc ccctatacta tttttgtata
    29521 aatggtcaat attaatggta tcatagatat atggaaaaga aattattaag aaaagaagtt
    29581 ttgataacct taaaatctca acctcaagct tacaaatcag aagttgactg taaactttta
    29641 gaggctttta taaaaactaa agcttatcaa aatagttgtg taattgctac ttacctatct
    29701 tttgactacg aatacaatac acaacttctt attaaacaag ctttatgtga tggcaaacgt
    29761 gtccttgttc ccaaaactta tccaaaaggt aaaatgattt ttgtggacta tcaaaaagat
    29821 aacttgagga caacaccgtt tggcttatta gaacctgtta atgatagagc tgttgaaaaa
    29881 gctagtatag acttaattca tgtgcccgga cttatcttta acaataaagg ttttcgtata
    29941 ggttatggtg caggttattt tgatcgctat ttgagtgatt ttgaaggtga tactattagt
    30001 actatttata ggtgtcagag acaggatttt gtagaagaga agcatgacgt agcagttaag
    30061 gaggtattat gtctatgaag aagttcgcta aagagtatcc gacgacagta ctcttggtca
    30121 gtctgacaac ccttgttttt ttattgatgc aactaactta tggaagtcaa gcggagtcta
    30181 gccaagttat ttttcaattt ggtggaatac aaggtgatta tctaaaagca tatccaacaa
    30241 atctatggag actaataagt cctatctttg ttcatattgg ttgggaacat tttttattaa
    30301 atggtttagc tctgtatttc gtaggacaaa tgggggaaag tatttgggga agtttgagat
    30361 ttctaattct ctatatttta tctggcttaa tgggtaatat ttttacgcta ttttttactc
    30421 cacatgtggt ggcagcagga gcctcaacgt ctttatttgg tgttttttct gctatagcaa
    30481 tcgcagggta ttttggaaaa aatccttatt taaaacaagt tggaaaatca tatcaagtga
    30541 tgatcttgtt aaatttattt tttaatatct tcacacccgg agtgagttta gcaggccatg
    30601 ttggtggttt agttggtgga gtattggtgg ctattttttt gacaaaacaa aatggttctt
    30661 tatta
//
LOCUS       DATRNK010000019        29746 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.019, whole genome shotgun sequence.
ACCESSION   DATRNK010000019 DATRNK010000000
VERSION     DATRNK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29746
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.019"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            57..1232
                     /locus_tag="VBC52_000864"
     CDS             57..1232
                     /locus_tag="VBC52_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6882462.1"
                     /translation="MTLEKRFNKYLDRIEVSLIRQFDQSISDIPGMVKLTLGEPDFTT
                     PDHVKEAAKSAIDANQSYYTGMSGLLALRQAAADFAKDKYNLTYNPDSEILVTIGATE
                     ALSASLIAILEAGDVVLLPAPAYPGYEPIVNLVGADIVEIDTRENDFRLTPEMLETAI
                     IQQGEKLKAVLLNYPTNPTGITYSRQEIAALAEVFKKYDIFVISDEVYSELTYTGQQH
                     VSIAEYLPNQTILINGLSKSHAMTGWRVGLVYAPEAFTAQIIKSHQYMVTAASTISQF
                     AGVEALSVGKNDTLPMRQEYIKRRDYIIDKMSKLGFKIIKPSGAFYIFAKIPDSYPQD
                     SFKFCQDFAYQQAVAIIPGVAFGKYGEGYIRLSYAASMEVIETAMARLKVFMESYEG"
     gene            1222..1983
                     /gene="recO"
                     /locus_tag="VBC52_000865"
     CDS             1222..1983
                     /gene="recO"
                     /locus_tag="VBC52_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEO6882463.1"
                     /translation="MRVSQTYGLVLYNRNYREDDKLVKIFTETEGKRMFFVKHASKSK
                     FNAVLQPLTIAHFILKINDNGLSYIDDYKEVLAFQEINSDLFKLSYASYITSLADVAI
                     SDNVADAQLFIFLKKTLELIEDGLDYEILTNIFEVQLLERFGVALNFHDCVFCHRAGL
                     PFDFSHKYSGLLCPNHYYKDERRNHLDPNMLYLINRFQSIQFDDLQTISVKPEMKLKI
                     RQFLDMIYDEYVGIHLKSKKFIDDLSSWGSIMKSD"
     gene            2046..2924
                     /locus_tag="VBC52_000866"
     CDS             2046..2924
                     /locus_tag="VBC52_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6882464.1"
                     /translation="MELIFRIFEMKKTLYITNKLFFANWFVPVIGAVLIQVIGIIVSR
                     RLVVKLLPDDYKFNLYIALLLFVPILASILLFLIYFQKRDFTSLGFTKDNNVLTTISG
                     FITGSLLMFFIYICHWLSGFVEISSSNSFSFGPFFFLFLAYFIQSLTEEILFRGYVMT
                     TVTKFKGSFAGVLCNSMLFSFIHFRNYGITAIALFNLFLLGIIFSILFNMTQNILFVT
                     GVHTTWNFTMGCVLGNKVSGGYSPVSLFRITENSSFALWNGGDFGFEGGVLCSLILVG
                     CLAYFSLERVRVSVRY"
     gene            3002..3994
                     /gene="plsX"
                     /locus_tag="VBC52_000867"
     CDS             3002..3994
                     /gene="plsX"
                     /locus_tag="VBC52_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEO6882465.1"
                     /translation="MKKIAVDAMGGDYAPKAIVEGVNQAISDFSDIEVQLYGDQKKIE
                     KYLTVTERVSIIHTEEKINSDDEPAKAVRRKKQSSMVLGAKAVKDGVAQAFISAGNTG
                     ALLAAGLFVVGRIKGVDRPGLMSTIPTLDGVGFDMLDLGANAENTASHLHQYAILGSF
                     YAKNVRGIEVPRVGLLNNGTEETKGDSLHKEAYELLAAEPSINFIGNIEARDLMSSVA
                     DVVVTDGFTGNAVLKTMEGTAMSIMGSLKSSIKSGGVKAKLGALLLKDSLYQLKDSMD
                     YSSAGGAVLFGLKAPIVKCHGSSDSKAVYSTLKQVRTMLETQVVDQLVDAFTDE"
     gene            4005..4243
                     /locus_tag="VBC52_000868"
                     /pseudo
     CDS             4005..4243
                     /locus_tag="VBC52_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085641.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
     gene            4367..5071
                     /locus_tag="VBC52_000869"
     CDS             4367..5071
                     /locus_tag="VBC52_000869"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEO6882466.1"
                     /translation="MNNQLIYTGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNMAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVTAGSFSK
                     RFGVEEGRVLETPIVEFYYKNDDLNDPFINDEHMKFLGIVNDEEIAYLKGETRRINEL
                     LKDWFAQIGLNLIDFKLEFGFDKDGKIILADEFSPDNCRLWDAEGNHMDKDVFRRDLG
                     SLTDVYQVVLEKLIAL"
     gene            5194..8919
                     /locus_tag="VBC52_000870"
     CDS             5194..8919
                     /locus_tag="VBC52_000870"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEO6882467.1"
                     /translation="MNKRIFVEKKADFDIKSASLVKELTHNLQLTSLKDLRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDCLLTETEITAELDKVAFFAIEALPGQFDQRAASSQE
                     SLLLLGSDSQVKVNTAQLYLVNKDIAEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPSLDFFETYKADDFAAYKAEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQATYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVRPSDVDTFIA
                     ACNKENIDAVVVATITAKPNLVMTWDGETIVDLERRFLDTNGVRVVVDAKVVDKDLTV
                     PEVRTTSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTTTASVMAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFGQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAAGENIYYIPGQAISEDIDFDLIKANFSQFETIQAQHKIT
                     AASAVKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIADSVKIG
                     QTQAAFTLTVNGNDLAGASLLSVFEGKLEEVYPTEFEQADALEEVPAVVSDTVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVAFVTLNEAAIADSVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNKKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLVGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQALFASAVKYFTGK"
     gene            9153..10607
                     /locus_tag="VBC52_000871"
     CDS             9153..10607
                     /locus_tag="VBC52_000871"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEO6882468.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNPSFMGKVKE
                     ALSTVKGGFAYLLMTEDKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDSGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAADEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEKTSFYIQKVK"
     gene            10635..11657
                     /locus_tag="VBC52_000872"
     CDS             10635..11657
                     /locus_tag="VBC52_000872"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEO6882469.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSQTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGDEVLPELEGKQLK
                     DVLLEPTRIYVKAALPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     ASVVIK"
     gene            11825..12373
                     /gene="purN"
                     /locus_tag="VBC52_000873"
     CDS             11825..12373
                     /gene="purN"
                     /locus_tag="VBC52_000873"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEO6882470.1"
                     /translation="MKIAVFASANGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAVVDLLDKHEIDLVCLAGYMKIVGETLLSAYEGRIINIHP
                     TYLPEFPGAHGIKDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVHVPRLADDSLESFET
                     RIHETEYQLYPAVLDSLGIKRK"
     gene            12396..13148
                     /locus_tag="VBC52_000874"
     CDS             12396..13148
                     /locus_tag="VBC52_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6882471.1"
                     /translation="MKLVKNLEIVESIFGDWDETIIWSCVQGIMGEVFVDSLDQPKSS
                     LAKLGRKSSFGFLAGQPTLFLLEVCSGEDIILVPQHKGWSDLIESTYGQNAHSFKRYA
                     TKKDTLFERSRLEKFVTQLPNGFELRAIDEKVYNSCLEKEWSQDLVANYATYQYYKKQ
                     GIGYVVYYQGNIIAGASSYSTYKNGIEIEVDTHPDFRRRGLATIVAAQLILTCLDKGI
                     YPSWDAHTRTSLNLSEKLGYEFSHEYIAYEID"
     gene            13168..14715
                     /gene="purH"
                     /locus_tag="VBC52_000875"
     CDS             13168..14715
                     /gene="purH"
                     /locus_tag="VBC52_000875"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEO6882472.1"
                     /translation="MTKRALISVSDKSGIIDFAKELKNLGWDIISTGGTKVALDDAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDVTYDLAVENIDIGGPSMLRSAAKNHASVTVVVDSADYATVLGELADA
                     SQTTFKTRQRLAAKAFRHTAAYDALIAEYFTAQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAEKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEATALEFAWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQESIDAANKHGLTMIFT
                     GVRHFRH"
     gene            14908..15807
                     /locus_tag="VBC52_000876"
     CDS             14908..15807
                     /locus_tag="VBC52_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEO6882473.1"
                     /translation="MNKWLVKASSLVVLGGMVLSAGSRVLADTYVRPIDNGRITTGFN
                     GYPGHCGVDYAVPTGTIIRAVADGTVKFAGAGANFSWMTDLAGNCVMIQHADGMHSGY
                     AHMSRVVARTGEKVKQGDIIGYVGATGMATGPHLHFEFLPANPNFQNGFHGRINPTSL
                     IANVATFSGKTQASAPSIKPLQSAPVQNQSSKLKVYRVDELQKVNGVWLVKNNTLTPT
                     GFDWNDNGIPASEIDEVDANGNLTADQVLQKGGYFIFNPKTLKTVEKPIQGTAGLTWA
                     KTRFANGSSVWLRVDNSQELLYK"
     gene            15954..17258
                     /locus_tag="VBC52_000877"
     CDS             15954..17258
                     /locus_tag="VBC52_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO6882474.1"
                     /translation="MKMNKKVLLTSTMAASLLSVASVQAQETDTTWTARTVSEVKADL
                     VKQDNKSSYTVKYGDTLSVISEAMSIDMNVLAKINNIADINLIYPETTLTVTYDQKSH
                     TATSMKIETPATNAAGQTTATVDLKTNQVSVADQKVSLNTISEGMTPEAATTIVSPMK
                     TYSSAPALKSKEVLAQEQAVSQAAANEQVSPAPVKSITSEVPAAKEEVKPTQTSVSQS
                     TTVSPASVAAETPAPVAKVAPVRTVAAPRVASVKVVTPKVETGASPEHVSAPAVPVTT
                     TSPATDSKLQATEVKSVPVAQKAPTATPVAQPASTTNAVAAHPENAGLQPHVAAYKEK
                     VASTYGVNEFSTYRAGDPGDHGKGLAVDFIVGTNQALGNKVAQYSTQNMAANNISYVI
                     WQQKFYSNTNSIYGPANTWNAMPDRGGVTANHYDHVHVSFNK"
     gene            17505..18203
                     /locus_tag="VBC52_000878"
     CDS             17505..18203
                     /locus_tag="VBC52_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEO6882475.1"
                     /translation="MPHLSKEAFKKQIKNGIIVSCQALPGEPLYTESGGVMPLLALAA
                     QEAGAVGIRANSVRDIKEIQEVTNLPIIGIIKREYPPQEPFITATMTEVDQLASLDIA
                     VIALDCTLRERHDGLSVVEFIQKIKRKYPEQLLMADISTFEEGKNAFEAGVDFVGTTL
                     SGYTDYSRQEEGPDIELLNKLCQAGIDVIAEGKIHTPKQANEINHIGVAGIVVGGAIT
                     RPKEIAERFISGLS"
     gene            18250..19566
                     /locus_tag="VBC52_000879"
     CDS             18250..19566
                     /locus_tag="VBC52_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6882476.1"
                     /translation="MSIKKSVIGFCLGAAALSMFACVDSSQSVMAAEKDKVEITWWAF
                     PTFTQEKAKDGVGTYEKKVIKAFEKKNPNIKVKLETIDFTSGPEKITTAIEAGTAPDV
                     LFDAPGRIIQYGKNGKLADLNDLFTDQFIKDVNNKNIIQASKSGDKAYMYPISSAPFY
                     MAFNKKMLKDAGVLKLVKEGWTTSDFEKVLKALKNKGYTPGSFFANGQGGDQGPRAFF
                     ANLYSAPITDKEVTKYTTDTKNSVKSMKKIVEWIKKGYLMNGSQYDGSADIQNFANGQ
                     TAFTILWAPAQPKTQAKLLESSKVDYLEVPFPSEDGKPDLEYLVNGFAVFNNKDENKV
                     KASKKFITFIADDKKWGPKDVIRTGAFPVRTSFGDLYKGDKRMMKISKWTQYYSPYYN
                     TIDGFSEMRTLWFPMVQSVSNGDEKPADALKDFTQKANDTIKKAAK"
     gene            19654..20541
                     /locus_tag="VBC52_000880"
     CDS             19654..20541
                     /locus_tag="VBC52_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6882477.1"
                     /translation="MRTNKLKMRETMIAYAFLAPILLFFLIFVFAPMVMGFVTSFFNY
                     SMTQFTFIGLANYNRMFHDSIFMKSLINTVIIVIGSVPVVVFFSLFVAANTYEKNVFS
                     RSFYRCVFFLPVVTGSVAVTVVWKWIYDPMSGILNYILKSGHVIEQNISWLGDKHWAL
                     LAIIIILLTTSVGQPIILYIAAMGNIDNSLCEAARVDGANEMQVFWQIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIALISFAQFKILGNDVEY"
     gene            20551..21381
                     /locus_tag="VBC52_000881"
     CDS             20551..21381
                     /locus_tag="VBC52_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO6882478.1"
                     /translation="MKKKTFSAYNFLTALILCLLTVLFIFPFYWIMTGAFKSQPDTII
                     IPPQWWPKAPTLENFKALTVQNPALRWLWNSVFISIMTMFLVCCTSSMAGYVLAKKRF
                     YGQKILFSLFIAAMALPKQVVLVPLVRIINFMGIHDTLWAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFINVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            21394..21837
                     /locus_tag="VBC52_000882"
     CDS             21394..21837
                     /locus_tag="VBC52_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEO6882479.1"
                     /translation="MIYDHLLNLTHYKDINPNLDLAIDYLLSHDLRNLDIGTYHISPE
                     VILMVQSNQLSESFDHIFEYHKKYLDIHYVIEGHEVIKLGKGDKVEVEEYLGDIGFIK
                     CSEETSFDLRDNYIAFFFPEEAHQPNGMGSLGNYVKKGVLKVLMA"
     gene            21857..22519
                     /locus_tag="VBC52_000883"
     CDS             21857..22519
                     /locus_tag="VBC52_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEO6882480.1"
                     /translation="MKKANQLIAAIFDVNNPFMQGCNVVFDLALLNLLFMITCLPLVT
                     IGAAKISLYRTLWQKLEGDQTNLLILYIKHLKKEWFQGMLLGLVELSILVVIIFDLTI
                     LHYQIGFIVSFLKITCYAFLLLTVMTSIYLFPMAARYEMSLLDTVKKSFIMACLNLKW
                     TGVLMFLLIMTWFIMVQSSLLFMLTVSAIFIFAYTAFAYFKIIILQKQFAYFSKQQGD
                     YQ"
     gene            22516..23433
                     /locus_tag="VBC52_000884"
     CDS             22516..23433
                     /locus_tag="VBC52_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEO6882481.1"
                     /translation="MKDLQKYQGIIPAFYACYDDKGDICPERVKALTNYFIDKGVQGL
                     YVNGSSGECIYQSVADRKLVLENVMSVAKGKLTVIAHVACNNTKDSVELAMHAEAIGV
                     DAIAAIPPIYFRLPEYAIADYWNTISQAAPQTDFIIYNIPQLAGVALTSDLYRKMLQN
                     PQVIGVKNSSMPVQDIQNFVAIGGENHIVFNGPDEQFLGGRLMGAAAGIGGTYGVMPE
                     LYLTLNQLIVDKDLEKARELQFTINDIITKLCSGHGNMYAVIKAVLEINEQLTIGSVR
                     LPLASVTEEDKPIIKEAAEMIRHAKKQFC"
     gene            23450..24331
                     /locus_tag="VBC52_000885"
     CDS             23450..24331
                     /locus_tag="VBC52_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO6882482.1"
                     /translation="MTRTVAIDIGGTMIKHGIVDNLGCIVEASELATEAYKGGPGILQ
                     KVCQIIDNYLAEGSIDGIAISSAGMVDPDEGCIFYSGPQIPNYAGTQFKKVLEDTYQV
                     RTEIENDVNCAGLAEAVSGSAKDSSIALCLTIGTGIGGCLIIDKTVFHGFSNSACEVG
                     YMHLSDGDFQDLASTTALIADVAKAHGDEISRWDGRRIFQEAKKGNEKCIASIDRMIN
                     YLGQGIANMVYVVNPEKVVLGGGIMAQKDYLQDKLSESLKRNLVTSLAEKTAIVFAQH
                     ENQAGMLGAYYHFKNRG"
     gene            24339..25316
                     /locus_tag="VBC52_000886"
     CDS             24339..25316
                     /locus_tag="VBC52_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO6882483.1"
                     /translation="MIETMSLDDMREYLGQDQIPEDFDDFWKKQTMKYQGNIEYRLDK
                     KDFNITFAQAYDLHFKGSNNSIVYAKCLFPKTNKPYPVVFYFHGYQNQSPDWSDQLNY
                     VAAGYGVVSMDVRGQAGQSQDKGHFDGITVKGQIVRGMISGPNHLFYKDIYLDVFQLI
                     DIIATLESVDSNQLYSYGWSQGGALALIAAALNPKIVKTVAVYPFLSDFRRVLDLGGV
                     SEPYDELFRYFKYSDPFHKTENNVLKTLAYIDVKNFAHRISCPVVLLTALKDDICPPS
                     TQFAIFNRLTSTKKHLLLPDYGHDPMTVQVKDHIFDQLTGSQFTKQKIE"
     gene            complement(25339..26142)
                     /locus_tag="VBC52_000887"
     CDS             complement(25339..26142)
                     /locus_tag="VBC52_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEO6882484.1"
                     /translation="MTIYDQIESALDLMTDLEREIACYFMGQPISKDALASTIVTKQL
                     HISQAALTRFAKKCGFKGYREFVFEYLKSHETISQQLYGLQNDNTKKVFMNYQEMISK
                     SADIIDEEQLLEVSHMIEQADRVYFYGKGSSSLVAKEFKIRLMRLGVICEALDDTDSF
                     SWTNSIVNDRCLVIAFSLSGNTNSVIGALKIASCHGAKTVLFTKQPHTIDYAFDKIIQ
                     VASARHLDYGNRISPQIPMLIMVDIIYAQFLDINKIEKERIFRETIIQR"
     gene            26424..27689
                     /gene="purD"
                     /locus_tag="VBC52_000888"
     CDS             26424..27689
                     /gene="purD"
                     /locus_tag="VBC52_000888"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEO6882485.1"
                     /translation="MKLLVVGSGGREHAIAKKLLASKDVDQVFVAPGNDGMTLDGLDL
                     VNIGISEHSRLIDFVKENEIAWTLIGPDDALAAGIVDGFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTFSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFANGDKFYIMPTAQDHKRAY
                     DGDKGLNTGGMGAYAPVPHLPQSVVDTAVETIVKPVLEGMIAEGRPYLGVLYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGAKFAENSKALLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIKE"
     gene            27970..28458
                     /gene="purE"
                     /locus_tag="VBC52_000889"
     CDS             27970..28458
                     /gene="purE"
                     /locus_tag="VBC52_000889"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEO6882486.1"
                     /translation="MQPIISIIMGSKSDWTTMQKTAEVLDNFGIAYEKKVVSAHRTPD
                     LMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSNE
                     LI"
     gene            28445..29536
                     /gene="purK"
                     /locus_tag="VBC52_000890"
     CDS             28445..29536
                     /gene="purK"
                     /locus_tag="VBC52_000890"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEO6882487.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPASDCPASRV
                     SEVIVAPYDDVEALGTLAARCDVLTYEFENVDADGLDAVVSAGQLPQGTDLLRISQNR
                     IFEKDFLANKAGVTVAPYKVVTSSLDLEGLDLTKTYVLKTATGGYDGHGQKVIRSAED
                     LPEAQQLANSAQCVLEEFVNFDLEISVIVSGNGQDVTVFPVQENIHRNNILSKTIVPA
                     RISDQLADKAKEMAVQIAKKLQLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPPIKLHAPAVMFNVLGQHVQQAIDHVAQNPSAHLHMYGKLE
                     AKHNRKMGHVTVFSDVPDEVEEFEERMDF"
BASE COUNT         9055 a         5227 c         6394 g         9070 t
ORIGIN      
        1 acacaaatta tttttcttgt ataattaaat atattatttc ttatcaggag gttatgatga
       61 cattagagaa acgatttaat aagtacttag atagaataga ggtctcctta attcgtcagt
      121 ttgatcaatc tatttcagat atacctggaa tggtaaaatt aactcttgga gaaccagatt
      181 ttaccactcc tgatcatgtt aaagaagcag ctaaatcagc tattgatgcg aatcaatcgt
      241 attacactgg tatgagcggt ttattagctc taaggcaagc tgctgcagat tttgcaaagg
      301 ataaatataa tcttacttat aatcctgata gtgagatctt ggttacgata ggtgccacag
      361 aagctctgtc tgcatctctt atagctattt tagaagctgg tgatgttgtt ctattacccg
      421 cccccgctta tccaggatat gagccaatag ttaaccttgt aggagctgat atcgtagaaa
      481 ttgatactag agaaaatgac tttagactaa ctcctgaaat gttagaaacg gctattatcc
      541 aacaaggtga aaaattaaag gctgttttac ttaattatcc tactaaccct acaggtatta
      601 cctattcaag gcaagaaatt gctgcattag ctgaagtctt taaaaaatat gatatttttg
      661 ttattagtga tgaagtttat tctgagttaa cttacactgg tcagcagcat gtttcaattg
      721 cagaatattt acctaaccaa actatcttaa ttaatggctt atcaaaatca catgctatga
      781 ctggttggag agttggattg gtgtatgctc ctgaagcatt tactgctcaa atcattaaaa
      841 gtcatcaata catggtgaca gctgctagta ctatttcgca atttgctggg gtagaagcct
      901 tatctgtggg taaaaatgat acgttaccta tgcgtcaaga atatattaaa cgtagagatt
      961 atatcattga taaaatgtct aaacttggtt tcaaaataat taaaccgagt ggcgcttttt
     1021 atatctttgc taaaatacct gatagttatc cacaggattc ttttaagttt tgtcaggatt
     1081 ttgcttatca acaagcagta gctattattc caggggtggc ttttggtaaa tatggtgaag
     1141 gatatatacg tctttcttat gctgctagta tggaagttat tgaaacagca atggctagat
     1201 tgaaagtttt tatggaatca tatgagggtt agtcaaacat acggtctcgt tttgtataat
     1261 cgtaattatc gtgaagatga taaattagtt aaaatcttta cggagactga gggaaaaagg
     1321 atgttttttg taaagcatgc ttcaaagtca aaattcaatg ctgtgctcca acctttgact
     1381 attgctcatt ttattttaaa aattaatgat aatggtcttt cttatattga tgattataaa
     1441 gaagttttag catttcaaga aattaattca gacttgttta agttgtcata tgcgagttat
     1501 attacttctt tggctgatgt ggctattagt gataatgtag cggatgctca attatttatt
     1561 ttcctaaaga aaacgttaga attgattgag gacggtttag attatgagat tctaacgaat
     1621 atttttgaag tacaactact agagaggttc ggtgttgctt taaattttca tgattgtgtt
     1681 ttttgtcata gggcaggatt accttttgat ttttcacaca aatattctgg attattatgt
     1741 ccaaaccatt attataaaga cgagagaaga aaccacctag atcctaatat gctatactta
     1801 atcaatcgtt ttcagtcaat tcaatttgat gatttacaaa caatttctgt gaaacctgag
     1861 atgaaactta aaattcgtca atttttggac atgatttacg atgaatatgt agggattcat
     1921 cttaaaagta aaaaatttat tgatgatttg tctagttggg gaagtattat gaaatcagat
     1981 taagttgtaa agaggaacat gacattagtg tcatgttctt ttttttaata ttaggataaa
     2041 ctaatatgga attaattttt aggatatttg agatgaaaaa aacattatac attaccaata
     2101 aactcttttt tgctaactgg tttgtgccag ttattggtgc agttctaatt caagttatcg
     2161 gcatcattgt aagcagacgc cttgtagtaa aattgctacc tgatgattat aaattcaatt
     2221 tatacattgc gcttttacta tttgttccta ttttggcaag tattttacta tttttaatct
     2281 atttccaaaa aagggatttt acttccttgg gatttactaa agataataat gttttaacaa
     2341 cgatctcagg atttattact ggtagtttac tgatgttttt tatttatatt tgccactggt
     2401 tatcaggttt tgtggaaatt tcttccagca attccttttc ttttggacct tttttctttt
     2461 tatttcttgc atactttatc caaagcttga cagaggaaat tttatttaga ggatatgtta
     2521 tgacaacagt taccaaattt aaaggtagtt ttgcaggtgt tttatgcaat tctatgttgt
     2581 tttcctttat ccactttaga aactatggta tcacagcaat agcacttttt aatttatttt
     2641 tacttggtat tattttttct attcttttta atatgaccca aaatattctt tttgttacag
     2701 gagttcatac tacatggaat tttactatgg gatgtgtatt aggtaataag gttagtgggg
     2761 ggtattctcc tgtttctctt tttagaatta ctgaaaattc tagctttgct ctttggaatg
     2821 gtggggattt tggttttgaa gggggagtac tatgttcttt gatattagta ggttgtttag
     2881 catacttttc tttggaaaga gtgagagtat ctgttagata ttagctatta ttgccttctt
     2941 gtgaaaaaaa taaagaaacg tgctataata tagccactaa gtttttatga ggagttcatt
     3001 catgaagaaa attgctgtag atgctatggg tggagattac gctcccaaag ctattgtaga
     3061 gggtgttaat caagctattt cagattttag tgatattgaa gtacaactct atggcgatca
     3121 gaaaaaaatt gagaaatatt taacagtaac ggagcgagtt agtattattc atactgaaga
     3181 aaaaattaat tctgatgatg agcctgctaa agctgttcgt cgtaaaaaac agtcttcgat
     3241 ggtcttagga gctaaggcag tcaaagatgg tgttgcacag gcctttattt ctgcagggaa
     3301 tacaggagct ttattagctg ctggtttgtt tgtagtagga cgtataaaag gtgttgatcg
     3361 accaggctta atgtcaacta tacctacgtt agatggtgtg ggttttgata tgcttgattt
     3421 aggagctaat gcagaaaaca cagcttctca tcttcatcaa tatgctattt tagggtcgtt
     3481 ttatgcaaaa aatgtccgtg gtattgaagt tcctcgtgta ggtctcttaa ataacggaac
     3541 ggaagaaaca aaaggcgatt ctcttcataa agaagcctat gaactgttag cagcagagcc
     3601 ttctattaat tttattggaa atattgaagc acgtgatttg atgtcaagtg tagcagatgt
     3661 tgttgtcact gatggtttta ctggaaatgc tgttctaaag acaatggaag gtacagctat
     3721 gagtattatg ggaagcctaa aatcttcaat taaatcaggt ggcgtgaaag ctaaattagg
     3781 agcacttcta ttaaaagata gcctatatca gctaaaagat agtatggact attcaagcgc
     3841 gggtggtgcg gttctttttg gtttaaaagc tccaatagta aaatgccatg gatcaagtga
     3901 ttctaaagct gtttattcta ctttgaagca agttcgtaca atgttggaaa ctcaagtagt
     3961 tgatcaatta gttgatgcct ttacagatga ataggagtga actcatgtca agagatgaag
     4021 tttttgaaaa gatgctggaa ttattaaggc agcaacttgg tgacccacag ttagatatca
     4081 caccagagtc atcgttacat gatgatttag caattgactc tatcgcttta acagagttta
     4141 ttatcatttg gaagatgtgt ttcatctaga gattccagat gaggctgtcg aacacatgtc
     4201 aagcgtccaa caactattag attatatcat tgaacataag taataataaa aaggaagaaa
     4261 atacgaacat ttcttctttt ttttgatttt aaataaagtt tttgttgtat tagataatgt
     4321 tgtgtgttag aataactctt aaaataagag aaaggcgaat aataaagtga ataaccagct
     4381 tatttataca ggaaaagcga aagatattta tagtacaaaa gatgaaaatg tgattagaac
     4441 ggtctataaa gaccaagcta ctatgttgaa tggagctcgt aaggagacta ttgatggcaa
     4501 gggagcacta aataatcaga tttcatctct tatttttgag aaattaaata tggctggtgt
     4561 ggtgacacat tatattgagc aaatttcaaa aaacgaacaa ttgaataaaa aagttgatat
     4621 tattccactt gaggtggtat tacggaatgt gacagcaggc tcattttcaa aacgttttgg
     4681 agtggaagaa ggacgcgttc ttgaaactcc aattgtggaa ttttactaca aaaatgatga
     4741 tctgaatgat ccttttatca atgatgagca tatgaagttt ttgggtattg ttaatgatga
     4801 ggagattgct tatctgaaag gagagactcg ccgtattaat gaactcttga aagattggtt
     4861 tgctcagatt gggcttaact tgattgactt caagttggag ttcggatttg acaaggatgg
     4921 caaaatcatc ttggctgatg agttctcacc tgacaactgc cgtctatggg atgctgaagg
     4981 aaatcatatg gacaaggatg tttttcgtag agatcttggt agcttgaccg atgtctatca
     5041 agttgtactt gagaagttga tagcattgta attagaagag gaaggaattg aatccgttcg
     5101 ggaaactcag aaaaaagatt gggcttactg aaatctcaat ttcagatatc ctcctatttt
     5161 tcagtcgctc ccttgtcgga cttggtatct tatatgaata aacgtatttt tgttgagaaa
     5221 aaggctgact ttgacattaa atcggctagt cttgtgaaag agttgacgca taatctacaa
     5281 ctgacttctt tgaaggattt gcgtattgtg caggtctatg atgtcttcaa tttggctgag
     5341 gatttgctgg cgcgtgctga gaagcatatt ttctctgagc aggtgacaga ctgtcttttg
     5401 acggaaactg aaatcactgc ggagcttgat aaggttgcct tctttgccat tgaggcgctt
     5461 cctggtcaat ttgaccaacg tgctgctagt tcgcaagaat ctttgctatt gcttggaagt
     5521 gacagtcagg ttaaggtcaa tacagcccag ctttacttgg tcaataagga tattgcagaa
     5581 gcagagcttg aagccgttaa gaactatctt ttgaatcctg ttgattcacg tttcaaggac
     5641 attaccttgc cgcttgaaga gcaggctttc tctgtgtctg ataagacaat ccctagtctt
     5701 gatttctttg aaacttataa ggctgacgat tttgcggcct ataaggcaga gcagggcttg
     5761 gctatggagg tcgatgatct tctcttcatc caagattatt tcaaatcaat cgggcgtgtg
     5821 ccaactgaga ctgagttgaa agttttggat acttactggt cagaccactg ccgtcacaca
     5881 acctttgaaa ctgagttgaa aaacatcgac ttttcagctt ctaaattcca aaaacaattg
     5941 caggcgactt atgacaaata tatcgccatg cgtgatgaac ttggtcgttc tgaaaagccg
     6001 cagacactta tggatatggc gactatcttt ggtcgttatg aacgtgccaa tggacgactg
     6061 gatgatatgg aagtctcaga tgaaatcaat gcctgctcag ttgaaattga agtagatgtt
     6121 gatggtgtaa aagagccttg gctcctcatg tttaagaatg agactcacaa tcacccaaca
     6181 gaaattgagc catttggtgg cgcagcaact tgtatcggtg gtgccattcg tgacccgttg
     6241 tcaggacgtt catacgttta tcaggctatg cgtatttcag gcgcaggcga tattacgact
     6301 ccgattgcgg aaacacgtgc tggtaaattg ccgcaacagg ttatttctaa aactgcggcg
     6361 cacggctatt cttcatatgg taaccagatt gggcttgcga caacttatgt gcgtgagtac
     6421 tttcaccctg gctttgtagc caaacgcatg gagcttggag ctgtggttgg tgctgcacct
     6481 aaggaaaatg tggttcgtga aaaaccagaa gcaggcgatg tggtcatctt gctcggtggt
     6541 aaaacaggtc gtgacggtgt cggcggtgcg acaggttcat ctaaggttca aacggttgaa
     6601 tctgtggaaa cagctggcgc agaggtacaa aaagggaatg ccatcgaaga acgtaagatt
     6661 caacgccttt tccgtaatgg taatgtcact cgcctaatca agaaatcaaa tgacttcggt
     6721 gcaggtggtg tctgtgttgc catcggtgaa ttggctgacg gtcttgaaat cgatttggac
     6781 aaggtaccgc ttaaatacca aggtcttaat ggtactgaaa ttgcaatctc agaatcacaa
     6841 gagcgtatgt cagtcgttgt tcgtcctagt gacgtggata ccttcatcgc agcctgcaac
     6901 aaggaaaata tcgatgccgt tgtggttgct actattactg caaaaccaaa ccttgtcatg
     6961 acttgggatg gtgaaacaat tgttgacttg gaacgccgtt tccttgatac caacggtgtc
     7021 cgtgtcgttg ttgatgctaa agtcgttgac aaagacttga cagttccaga ggtacgcaca
     7081 acatcagcag agacacttga agcggatacg cttaaggtct tgtctgacct caaccacgct
     7141 agtcaaaaag gtctccaaac tatctttgac tcatctgttg gtcgctcaac cgttaaccac
     7201 ccaatcggtg gccgttacca aatcacacca acagaaagct ctgttcaaaa attgccagtt
     7261 caacacggtg tcacaacaac tgcatctgtt atggctcaag gttacaatcc ttatattgca
     7321 gaatggtcac cttaccacgg tgctgcctat gctgtcattg aagcgacagc tcgcttggta
     7381 gcaacgggtg ctgactggtc tcgtgcacgt ttctcttacc aagagtattt cgagcgtatg
     7441 gataaacagg cagagcgttt tggtcagcca gtatcagctc ttcttggttc tattgaggct
     7501 cagattcaac ttggattgcc atcaatcggc ggtaaggact ctatgtctgg tactttcgaa
     7561 gaattgacag taccgccaac cttggtagct ttcggtgtga caacagcgga cagccgcaag
     7621 gttctctctc ctgagtttaa agcggctggt gagaatatct actacattcc aggtcaagct
     7681 atttcagaag atattgattt tgaccttatc aaggctaact ttagccagtt cgagactatt
     7741 caagctcaac ataagattac tgctgcttca gctgttaaat acggtggtgt cctagaaagt
     7801 cttgctctca tgacttttgg taaccgtatc ggggcctctg ttgaaattgc agagcttgac
     7861 agtagcttga cagctcaact cggaggcttt gtctttacat cagctgagga aattgctgac
     7921 tcggtgaaaa tcggtcaaac tcaggcagcc tttacactta ctgtcaatgg aaatgacctt
     7981 gctggggcaa gccttctatc agtcttcgaa ggcaaattgg aagaggttta cccaacagag
     8041 tttgaacagg cagacgctct tgaagaagtt cctgctgtgg tatcagatac tgttatcaag
     8101 gctaaggaaa caattgaaaa accagtggtt tacattccag tcttccctgg taccaactca
     8161 gaatatgatt cagctaaggc ctttgaacag gttggagcta gtgtcaactt ggtagcattt
     8221 gtaaccttga atgaggctgc tattgctgac tcagttgaca caatggtcgc taatattgct
     8281 aaggcaaata tcatcttctt tgctggagga ttctcagcag cggatgaacc agatgggtct
     8341 gctaagttta tcgtcaatat cttgcttaac aagaaggttc gcgcagctat tgacagcttc
     8401 atcgaaaaag gtggccttat catcggtatc tgtaatggtt tccaagccct tgtcaaatca
     8461 ggtcttcttc catacggaaa cttcgaggaa gctggtgaga caagtccaac cctcttctat
     8521 aacgatgcca accagcacgt tgccaagatg gttgagactc gtatcgcaaa taccaactca
     8581 ccttggttgg taggagttga ggtcggcgat attcatgcca tcccagtttc acacggtgaa
     8641 ggtaaatttg ttgtcagcgc ttctgaattt gcagagctaa gagacaatgg tcaaatctgg
     8701 agccaatatg tggactttga cggacaacca tctatggatt ctaaatacaa tccaaatggc
     8761 tctgtcaatg ccatcgaagg gattaccagc aagaatggtc aaatcatcgg taagatgggg
     8821 cactcagaac gctgggaaga cggcctcttc caaaatatcc ctggtaacaa agaccaagcc
     8881 ttgtttgcta gtgcagtaaa atattttaca ggaaaataat tttttcaaat tttttcataa
     8941 aacagataag gaaacaagag cgaagcgagc ttaactgtat ctttctgtcg tagtggtatt
     9001 tgggagccgt tgaggctaag actcaatgat tagcttgaaa atagcttgat agattttcta
     9061 ttttgatgtt ggcattggtg acgatagatc caaaattgag aaaatcatca aagctactta
     9121 agatgagata caaaaagaat taggaaaaaa gaatgacata cgaagtaaaa tctctaaatg
     9181 aagaatgtgg agtctttggt atctgggggc atcctcaggc agctcaagtc acttactttg
     9241 ggcttcatag ccttcaacat cgcggtcaag aaggtgctgg tattgtttcg aatgataatg
     9301 ggaaactcta tggttatcga aatgttgggc tcctttctga agtttttaag aatcaatctg
     9361 aattagataa tttaactggg aatgcggcta ttggacatgt tcggtatgct actgcaggtt
     9421 ctgcagatat tcgcaatatt cagccttttc tttataaatt tcatgacggg caatttgctt
     9481 tatgccataa tggtaatttg acaaatgcta tttctttaag gaaagaatta gaaaagcaag
     9541 gtgcaatttt caatgcctcc tcagatactg aaattttgat gcacttgatt cgtcgaagcc
     9601 ataacccaag ttttatgggg aaggtaaaag aagctttaag cactgtaaag ggaggcttcg
     9661 cctatctact gatgacagaa gataaattaa ttgctgctct tgaccctaat gcctttcgtc
     9721 ctttgtcaat tggacaaatg caaaatggtg cttgggttat ttccagtgag acctgtgctt
     9781 ttgaggtggt aggcgcaaaa tgggttagag atgttgaacc tggcgaagtt atccttatag
     9841 acgatagtgg tattcaatgt gatcgttata ctgatgaaac gcaactggca atttgttcga
     9901 tggaatatgt ctattttgca aggccggatt caactataca tggtgttaat gttcatacgg
     9961 ctcgaaaaaa tatgggaaag cgtcttgcac aagaatttaa acaggatgct gatattgtaa
    10021 ttggtgtccc aaattcgtcc ttatcggctg ctatgggctt tgctgaagaa tccggattac
    10081 caaatgagat gggtcttgta aaaaatcagt atacgcagcg aacctttatt caaccgacac
    10141 aagaattaag ggaacaaggt gttcgaatga aactatcagc ggtatctggt gttgtcaaag
    10201 gaaagcgcgt tgttatgatt gatgactcaa ttgtaagagg aacgacttct agaaggattg
    10261 tcggattatt aagagaagca ggagctagtg aagtacatgt tgctatagct agtccagaat
    10321 taaagtatcc ttgtttttat ggtattgata ttcaaacaag acgagaatta atttcagcaa
    10381 atcacgctgc tgacgaagtg tgtgatatta ttggcgcaga tagtctgact tatctctcac
    10441 tagatgggtt gattgaatca attggactcg aaacaaaagc accaaatggt ggtttatgcg
    10501 tagcttactt tgatggacat tatccaacgc cgctttatga ttatgaagaa gaatacctca
    10561 gaagtttaga agaaaaaaca agtttttata ttcaaaaagt gaaataagct ctgctaatta
    10621 tataaggaga aaacatgtct gaaaaaaatg cttatgcaaa atctggtgtt gatgttgaag
    10681 ctggttatga agtggttgaa agaataaaaa aacacgttgc tcgtacagaa cgtgcgggag
    10741 tcatgggagc tctaggtggt tttggtggga tgtttgactt gtcacaaaca ggtgttaaag
    10801 aacctgtctt gatttcaggg actgacggtg tcggaacaaa actcatgctt gctatcaagt
    10861 acgacaagca cgacacaatc ggtcaagact gtgttgccat gtgtgtcaat gatattattg
    10921 cagcaggtgc agagcccctt tacttccttg actatgtagc gactggtaaa aacgaacctg
    10981 ccaaattgga acaggttgtc gctggcgttg cagaaggttg tgttcaagca ggagcagcac
    11041 tcattggagg tgagaccgct gaaatgcctg ggatgtatgg cgaagatgat tatgaccttg
    11101 ctggctttgc tgttggtgtg gctgaaaaat ctcaaatcat cgacggttca aaagtaaaag
    11161 aaggagatat tcttcttgga ctggcttcaa gtggtatcca ctcaaatggt tattcattgg
    11221 tgcgtcgtgt ctttgctgac tacactggtg atgaggtgct tccagagctt gaaggcaaac
    11281 aactcaagga tgttcttctt gagccaactc gtatctatgt taaagcagct ctgccattga
    11341 tcaaggaaga actggtcaac ggtatcgccc acatcacagg tggtggtttt atcgagaatg
    11401 ttcctcgtat gtttgccgat gatttggctg cggaaatcga tgaggataag gtgccagtac
    11461 ttccgatttt caaggcgctt gaaaaatatg gtgacatcaa gcacgaagaa atgtttgaaa
    11521 tcttcaatat gggtgtcggt cttatgctag ctgttagtcc tgaaaatgtt aaccgcgtca
    11581 aagagctctt ggacgaacca gtttatgaaa ttggtcgtat catcaagaag gctgatgcta
    11641 gtgtggtgat taaataagtc actaaatttt ataggaaagg agtgtttagt tagtttgcct
    11701 aactgaacac gggattaaat ttctaggaaa aaagataaaa cgacttagac gcaagcgtcg
    11761 ttgttcgttt tcctattttt ctacgaaatt tttcggcaag ccgaatcatc cctttgtatc
    11821 gaatatgaaa atcgctgttt ttgcttctgc taatggttcc aactttcagg tcatagcaga
    11881 gcagtttcca gttagttttg tcttttcaga tcatcgtgat gcctatgttt tagagcgtgc
    11941 tcagaactta gccattccaa gcttcgcttt tgaactcaaa gagtttgaga ataaggcggc
    12001 ttacgagcaa gccgtcgttg atttgttgga caaacacgag attgacttgg tctgtctggc
    12061 gggctacatg aagattgtcg gagaaacctt gctctcagcc tatgaagggc gtatcatcaa
    12121 tattcaccca acctatctgc ctgaatttcc aggtgctcac ggtatcaagg atgcttggga
    12181 ggcaggtgtt gaccagtctg gtgtgaccat ccattgggtg gactctggtg tggataccgg
    12241 tcaggtcatc caacaagtgc acgtgccacg cctagcagat gatagtctag aaagctttga
    12301 aacccgcatc cacgaaaccg aataccaact ctacccagct gtcttagata gcttgggcat
    12361 aaagagaaaa tagtaaaatt tgttataata agctaatgaa actagtgaaa aatttagaga
    12421 tagtagagtc tatttttggt gattgggacg agaccataat ctggtcgtgt gtccaaggta
    12481 ttatgggaga agtttttgta gatagtttag accaaccaaa atcttctttg gcaaaattgg
    12541 gaaggaaatc atcttttggt tttctagcag gtcagcctac tttatttcta ctagaagttt
    12601 gctctgggga ggatattatc cttgttcctc agcataaagg atggtcagat ttgattgaat
    12661 caacttatgg tcagaatgct cattctttta agcgttatgc gacgaaaaaa gatactttgt
    12721 ttgagcgaag taggttagag aagtttgtta ctcagttgcc aaatggtttt gaattacgtg
    12781 ctattgatga gaaagtttac aactcttgct tagaaaagga atggtcacag gatttagtag
    12841 caaattacgc tacttatcag tactataaaa aacaaggtat aggttatgtt gtatattatc
    12901 aggggaatat cattgcagga gcttcatctt attcaaccta taaaaatggg attgagatag
    12961 aagtagatac gcatccagat tttcgtcggc gtggtttagc aacaatagtt gcagctcagt
    13021 taatactaac ttgtttagat aagggaattt atcctagctg ggatgctcat acaaggactt
    13081 cattgaattt atctgaaaaa ctcggttatg aattttctca tgaatacata gcctatgaaa
    13141 tagattgaag taagaaaagg agaactcatg actaaacgtg ctttaatctc agtttctgac
    13201 aaatcaggaa ttattgactt tgcaaaagaa ttgaaaaact tgggttggga tattatctca
    13261 actggtggga ctaaggttgc ccttgatgat gctggtgttg agaccattgc catcgacgat
    13321 gtgactggat tcccagaaat gatggacggt cgtgttaaga ccctccaccc aaacattcac
    13381 ggtgggcttc tggctcgtcg cgacgctgac agccaccttc aggctgctaa ggacaacaat
    13441 attgagttga ttgacctcgt ggttgtcaac ctctatccct tcaaggagac catccttcgc
    13501 ccagacgtga cctacgattt ggcggtggaa aatatcgaca tcggcggtcc atcaatgctt
    13561 cgctcagctg ctaaaaacca cgctagcgta accgttgtgg ttgattcagc tgactatgcc
    13621 actgttttgg gagaattggc tgacgctagt cagacgacat ttaaaactcg tcaacgcttg
    13681 gcagctaagg ccttccgtca tacggcagcc tacgacgctt tgattgctga gtacttcaca
    13741 gctcaagtgg gagaggctaa gcctgaaaaa ttgaccatca cttatgacct taaacaggct
    13801 atgcgttacg gagaaaatcc acagcaagac gctgatttct accaaaaagc cttaccaaca
    13861 gactactcaa tcgcttcagc taaacagctc aatggaaaag aattgtcctt caataatatc
    13921 cgtgatgctg atgcagcaat ccgtattatc cgcgatttca aagacagtcc aacggttgtt
    13981 gccctcaaac acatgaaccc atgtggtatc ggacaggctg atgatattga gacagcttgg
    14041 gattacgctt atgaagctga tccagtttca atctttggtg gaattgttgt ccttaaccgt
    14101 gaagttgacg cagcgacagc tgagaagatg caccctatct tcttggaaat catcatcgca
    14161 ccatcatact cagaagaagc gctagctatt ctcacaaata aaaagaaaaa cttgcgtatc
    14221 cttgagttgc cgtttgatgc ccaagctgcc agcgaagtgg aagctgagta cactggcgta
    14281 gttggtggac ttttggtgca aaaccaagac gttgtggctg aaaatccatc tgactggcaa
    14341 gtggtgacag accgccagcc aacagaacaa gaggcgactg cccttgagtt tgcctggaag
    14401 gctatcaagt atgttaagtc taacgggatt attattacta acgatcacat gacgcttgga
    14461 ctcggtgcag gtcaaaccaa ccgtgtcggc tcagtcaaga ttgctatcga gcaggctaag
    14521 gaccaccttg acggtgccgt tctagcatca gatgccttct tcccatttgc ggacaacatt
    14581 gaagaaatcg ctgccgcagg gatcaaagca atcatccaac caggtggttc agttcgtgac
    14641 caagaatcta ttgacgccgc aaacaaacat ggcttgacca tgatcttcac aggcgtgaga
    14701 cattttagac attaaaaaat agtagtttag gttttcttaa gttaactcag gtatactacg
    14761 tatatcctaa aaacttttct ttttattaaa cctcctaagt actcatcaag gtgagtactt
    14821 tttttgatat tttgatttaa attttcgaat aagttaattg caagtgtttg agcacttcat
    14881 tatactaatt ctatttggag gtttttcttg aataaatggt tagttaaggc aagttcctta
    14941 gttgttttag gtggtatggt tttatctgcg ggttcccgag ttttagcgga tacttatgtc
    15001 cgtccaattg ataatggtag aattacaaca ggtttcaatg gttatcctgg acattgtggg
    15061 gtggattatg ctgttccgac tggaacgatt attagggcag tggcagatgg tactgtgaaa
    15121 tttgcaggag ctggagccaa cttttcttgg atgacagact tagcaggaaa ttgtgtcatg
    15181 attcaacatg cggatggaat gcatagtggt tacgctcata tgtcacgtgt ggtggctagg
    15241 actggggaaa aagtcaaaca aggagatatc atcggttacg taggagcaac tggtatggcg
    15301 acgggacctc accttcattt tgaattttta ccagctaacc ctaattttca aaatggtttc
    15361 catggacgta tcaatccaac gtcactaatt gctaatgttg cgacctttag tggaaaaacg
    15421 caagcatcag ctccaagcat taagccatta caatcagctc ctgtacagaa tcaatctagt
    15481 aaattaaaag tgtatcgagt agatgaatta caaaaggtta atggtgtttg gttagtcaaa
    15541 aataacaccc taacgccgac tgggtttgat tggaacgata atggtatacc agcatcagaa
    15601 attgatgagg ttgatgctaa tggtaatttg acagctgacc aggttcttca aaaaggtggt
    15661 tactttatct ttaatcctaa aactcttaag actgtagaaa aacccatcca aggaacagct
    15721 ggtttaactt gggctaagac acgctttgct aatggtagtt cagtttggct tcgcgttgac
    15781 aacagtcaag aactgcttta caaatagttt gaggtattga ttcattgttt taaatgacag
    15841 ttgtgttact aactaagtac aatttcttta aaccgtctga aaataatttt atagtccagt
    15901 aaagtgtgat attatagtct cggactaata aaaaggaaat aggaattgaa gcaatgaaaa
    15961 tgaataaaaa ggtactattg acatcgacaa tggcagcttc gctattatca gtcgcaagtg
    16021 ttcaagcaca agaaacagat acgacgtgga cagcacgtac tgtttcagag gtaaaggctg
    16081 atttggtaaa gcaagacaat aaatcatcat atactgtgaa atatggtgat acactaagcg
    16141 ttatttcaga agcaatgtca attgatatga atgtcttagc aaaaataaat aacattgcag
    16201 atatcaatct tatttatcct gagacaacac tgacagtaac ttacgatcag aagagtcata
    16261 ctgccacttc aatgaaaata gaaacaccag caacaaatgc tgctggtcaa acaacagcta
    16321 ctgtggattt gaaaaccaat caagtttctg ttgcagacca aaaagtttct ctcaatacaa
    16381 tttcggaagg tatgacacca gaagcagcaa caacgattgt ttcgccaatg aagacatatt
    16441 cttctgcgcc agctttgaaa tcaaaagaag tattagcaca agagcaagct gttagtcaag
    16501 cagcagctaa tgaacaggta tcaccagctc ctgtgaagtc gattacttca gaagttccag
    16561 cagctaaaga ggaagttaaa ccaactcaga cgtcagtcag tcagtcaaca acagtatcac
    16621 cagcttctgt tgccgctgaa acaccagctc cagtagctaa agtagcaccg gtaagaactg
    16681 tagcagcccc tagagtggca agtgttaaag tagtcactcc taaagtagaa actggtgcat
    16741 caccagagca tgtatcagct ccagcagttc ctgtgactac gacttcacca gctacagaca
    16801 gtaagttaca agcgactgaa gttaagagcg ttccggtagc acaaaaagct ccaacagcaa
    16861 caccggtagc acaaccagct tcaacaacaa atgcagtagc tgcacatcct gaaaatgcag
    16921 ggctccaacc tcatgttgca gcttataaag aaaaagtagc gtcaacttat ggagttaatg
    16981 aattcagtac ataccgtgcg ggagatccag gtgatcatgg taaaggttta gcagttgact
    17041 ttattgtagg tactaatcaa gcacttggta ataaagttgc acagtactct acacaaaata
    17101 tggcagcaaa taacatttca tatgttatct ggcaacaaaa gttttactca aatacaaaca
    17161 gtatttatgg acctgctaat acttggaatg caatgccaga tcgtggtggc gttactgcca
    17221 accactatga ccacgttcac gtatcattta acaaataata taaaaaagga agctatttgg
    17281 cttctttttt atatgccttg aatagacttt caaggttctt atataatttt tattaaattg
    17341 aggagattaa gctataagtc tgaaactact ttcacgttaa ccgtgactaa atcaaaacgt
    17401 taaaactaaa atctaagtcc gtaaagatta ttgaaaacgc tttaaaaata gatataataa
    17461 ggtttgtaga tatctaaaat taaaaaagat aaggaagtga gaatatgcca catctaagta
    17521 aagaagcttt taaaaagcaa ataaaaaatg gcattattgt gtcatgtcaa gctttgcccg
    17581 gggagcctct ttatactgaa agtggaggtg ttatgcctct tttagctttg gcagctcaag
    17641 aagcaggagc ggttggtata agagccaata gtgtccgcga cattaaggaa attcaagagg
    17701 ttactaattt acctatcatc ggcattatta aacgtgaata tcctccacaa gaaccattta
    17761 tcactgctac gatgacagag gtggatcaat tagctagttt agatattgca gtaatagcct
    17821 tagattgtac acttagagag cgtcatgatg gtttgagtgt agttgagttt attcaaaaga
    17881 taaaaaggaa atatcctgaa cagttgttaa tggctgatat aagtactttt gaagaaggca
    17941 aaaatgcttt tgaagcagga gttgattttg tgggtacaac tctatctgga tacacagatt
    18001 acagccgcca agaagaagga ccggatatag aactccttaa taagctctgt caagccggta
    18061 tagatgtgat tgcggaaggt aaaattcata ctcctaagca ggctaatgaa attaatcata
    18121 taggtgttgc aggaattgta gttggtggtg ctatcactag accaaaagaa atagcggagc
    18181 gtttcatctc aggacttagt taaaagtgtt actcaaaaat caaaatcaaa ataaaaaagg
    18241 ggaatagtta tgagtatcaa aaaaagtgtg attggttttt gcctcggagc tgcagcatta
    18301 tcaatgtttg cttgtgtaga cagtagtcaa tctgttatgg ctgccgagaa ggataaagtc
    18361 gaaattacgt ggtgggcttt tccaaccttt actcaagaaa aggctaagga tggagtaggt
    18421 acttatgaga aaaaagtcat caaggctttt gaaaagaaaa atcctaatat aaaagtaaaa
    18481 ctagagacaa ttgatttcac atctggacct gaaaaaatca ctacagcgat tgaagcaggg
    18541 acagcacctg atgtgctttt tgatgcacca ggacgaatta ttcaatatgg taaaaatggt
    18601 aaattagcag atttgaatga tttatttaca gaccaattta ttaaggatgt caataataag
    18661 aatatcattc aagcttctaa gtctggcgat aaagcctaca tgtatccaat aagttctgcc
    18721 ccattttata tggcgttcaa taaaaaaatg cttaaagatg caggagtttt gaaactcgta
    18781 aaagaaggtt ggactactag tgattttgaa aaagtactaa aagcactaaa aaataaaggc
    18841 tatacaccag gttcattctt tgcaaacggg caaggaggag atcaaggacc acgtgcattt
    18901 tttgctaatc tttatagtgc tccaataaca gataaagaag taacaaaata taccactgac
    18961 actaaaaatt ctgtaaaatc aatgaaaaaa atagttgaat ggattaagaa aggctacttg
    19021 atgaatgggt ctcagtatga tggctcagct gacattcaaa actttgccaa tggacaaact
    19081 gctttcacta tcctatgggc tccagctcaa cccaaaactc aagcaaaatt attagagtca
    19141 agtaaagtgg attaccttga agtgccattc ccatcagaag atggaaaacc agatttagaa
    19201 taccttgtta atggttttgc ggtctttaat aataaagatg aaaacaaagt aaaagcctct
    19261 aagaaattta tcacttttat tgctgacgat aaaaaatggg gaccaaaaga tgttatacgt
    19321 acaggtgctt tcccagttag aacatcattt ggagatcttt ataaaggtga taaacgtatg
    19381 atgaagattt caaaatggac tcaatattat tcaccatatt acaacactat cgatggattt
    19441 tctgaaatga gaaccttatg gttcccaatg gttcaatctg tatccaatgg tgacgaaaaa
    19501 ccagcagatg ctttgaaaga ctttactcaa aaagcaaatg ataccattaa aaaagcagct
    19561 aaataattaa ggagactcct tttctatggt caatcacata ttgggataga agaggagttt
    19621 ttagtcaagt taagatgtaa aggagagtac cgtatgcgta ctaataagtt aaagatgagg
    19681 gaaacgatga tagcttatgc ttttctagca cctattctct tatttttctt aatttttgtt
    19741 tttgcaccga tggttatggg ctttgtcact agttttttta attattctat gacacagttc
    19801 acttttattg ggttagctaa ttacaatcgt atgtttcatg attctatttt catgaaatcc
    19861 ttgataaata cagtaatcat tgtgattggc tctgtgccag tagtggtttt cttctcactt
    19921 tttgttgctg ccaatactta tgaaaaaaat gttttttcac gctctttcta ccgttgtgtc
    19981 ttcttcttgc cagtagtaac tggtagtgtt gcagttacag ttgtttggaa atggatttat
    20041 gaccctatgt ccggaatttt aaattacatc ttgaaatcag gtcatgtcat tgaacagaat
    20101 attagctggc ttggggataa gcactgggca ttgcttgcta ttattattat tttgctaacg
    20161 acctcagttg gtcaacctat tattctatat attgcggcga tgggaaatat tgataattct
    20221 ctttgcgaag ctgcgcgtgt tgatggagcc aatgaaatgc aagttttttg gcaaattaaa
    20281 tggccgagtc tcttaccaac tacactctat attgcagtta taacaacaat taattcattt
    20341 caatgttttg ccttaatcca gctcctaaca tctggtggac caaattattc aacaagcact
    20401 ttgatgtatt atctttatga aaaagccttt aaactatcag aatatggcta tgctaatact
    20461 atgggagtat tcctagcagt aatgattgct ttaataagtt ttgctcaatt taagatctta
    20521 ggaaatgatg tggaatatta ggaggaaaac atgaaaaaga aaacattcag tgcttataac
    20581 tttttaacgg ctcttatcct ttgtcttttg acagtgcttt ttatctttcc attttattgg
    20641 attatgacag gagcttttaa atctcaacct gatactatta tcatcccccc acaatggtgg
    20701 cccaaagcac cgaccttaga aaattttaaa gctttaactg tacaaaaccc agctttgaga
    20761 tggctttgga atagtgtctt tatctcaatt atgacaatgt tcctagtttg ctgtacatcg
    20821 tcaatggcag gctatgtttt agctaagaaa cgattttacg gtcaaaaaat attgttctcc
    20881 ctttttattg ctgctatggc tttacctaaa caagttgttt tagtgccatt agttagaatt
    20941 atcaatttta tggggataca tgatacctta tgggcggtta ttttacctct tgttggttgg
    21001 ccttttgggg tcttcttgat gaaacagttt tctgaaaata ttccaacaga attactggaa
    21061 tcagctaaaa ttgatggttg tggagagatt cgaacattta ttaatgtagc atttccaatt
    21121 gtcaaacctg gatttgctgc cctagctatt ttcacattta tcaatacttg gaatgactat
    21181 tttatgcaat tagttatgct aacttctaga aataatctga ccatttctct tggggtagct
    21241 actatgcagg ctgaaatggc aacaaactat ggcttaatta tggcaggtgc agccttagct
    21301 gctgttccta ttgtgacagt attcttagtt ttccaaaaat cctttactca ggggattaca
    21361 atgggagctg ttaaaggata ggtaggattt attatgattt acgatcattt attgaattta
    21421 acgcattata aagatattaa tcctaattta gatttagcga ttgattatct actaagtcat
    21481 gatttaagga atttagatat aggcacctat catattagcc cagaagtgat actcatggtc
    21541 caatcaaatc aacttagtga gtcatttgat catatttttg aataccataa aaaatacctt
    21601 gatatacatt atgtcattga aggacatgaa gttatcaaat tgggaaaagg tgataaggta
    21661 gaggtggagg aatacttagg cgatatagga tttatcaagt gctcagaaga gacttctttt
    21721 gatttgagag ataattatat agccttcttt ttcccagaag aagctcatca gccaaatggt
    21781 atgggaagtc taggtaatta tgtcaaaaaa ggggtattga aagtgttaat ggcgtaagaa
    21841 atacagggag tatattgtga aaaaagcaaa tcaattaata gcagcgattt ttgatgttaa
    21901 caaccccttt atgcaaggtt gcaatgttgt ttttgatctg gcattactaa accttctctt
    21961 tatgataact tgcctaccat tggtaactat tggtgctgct aaaattagtt tgtatcgaac
    22021 gttgtggcaa aaattagaag gtgatcagac aaatctttta atattgtata taaaacacct
    22081 aaaaaaagag tggttccaag gtatgctttt aggtttggtt gaactaagta ttttggttgt
    22141 gatcatattt gatttaacaa ttcttcatta tcaaataggt ttcattgtgt ctttcttgaa
    22201 gataacttgt tacgcctttt tgttattgac tgtgatgacg agcatatacc tatttcctat
    22261 ggctgcccgc tacgaaatgt ctcttttaga taccgtgaaa aagagcttta taatggcatg
    22321 tttaaacttg aaatggactg gtgtattgat gtttttatta atcatgactt ggtttattat
    22381 ggtgcagtct agtctcttat ttatgcttac agtgtcagct atatttattt ttgcttatac
    22441 cgcctttgct tactttaaaa ttattatttt acaaaaacaa tttgcctatt tcagcaaaca
    22501 acaaggagat tatcaatgaa agatttacaa aaatatcaag gaattattcc tgcattttat
    22561 gcttgctatg atgataaggg tgatatttgt cccgaacgag taaaagccct gaccaattat
    22621 tttattgata aaggtgttca aggactttat gttaatggtt catccggaga atgtatttac
    22681 caaagtgtag cagatcgtaa acttgtactg gaaaatgtta tgtccgttgc taagggtaaa
    22741 ctaacagtaa tagcgcatgt ggcatgtaat aatactaagg atagcgtaga gttggctatg
    22801 catgctgaag ctataggtgt tgatgctatt gcagcaattc caccaattta ttttcgatta
    22861 ccagagtatg ccatagcaga ttattggaat actattagtc aagcagcacc acaaactgat
    22921 tttattattt acaatattcc tcaattggca ggggttgctt tgacatcgga tttatatcga
    22981 aaaatgttac aaaatccgca agtaattggt gttaaaaatt cttccatgcc agttcaagac
    23041 attcagaatt ttgtggctat tggaggagaa aatcatattg tttttaatgg tccagatgaa
    23101 caatttttgg gtggtcgtct catgggagcc gctgcaggta ttggtggtac ctatggcgta
    23161 atgccagaat tatacttgac tttaaatcag ttaattgttg ataaagattt agaaaaagcg
    23221 cgtgaacttc aatttacaat taacgatatt atcacaaagc tttgttcggg tcatggtaat
    23281 atgtatgctg tgatcaaagc tgtattggaa atcaatgagc aactaactat aggttccgtt
    23341 cggttacctt tagcatctgt aacagaagaa gataaaccta ttattaaaga ggcagcagaa
    23401 atgattagac atgctaaaaa acagttttgt taaagtgagg tagataatta tgaccagaac
    23461 agtagcaatt gatattggcg gtacaatgat taagcatggc attgtggata atttaggttg
    23521 tattgttgag gcaagtgagc ttgccacaga agcctacaaa ggtggtccag gcattttaca
    23581 aaaagtttgt caaattatcg ataactatct agcagaaggg agcattgatg gaattgctat
    23641 ctcatctgct ggcatggtag atccagatga aggatgtata ttttactctg gtcctcaaat
    23701 tccaaactat gctgggacac aatttaagaa agttttagaa gacacttatc aagtacgcac
    23761 agaaattgaa aatgatgtta attgtgcagg attagctgaa gctgtctcag gtagtgccaa
    23821 agatagttca atagcgcttt gtcttactat tggaacaggt attggtggtt gtctgattat
    23881 tgataaaaca gtttttcatg gctttagtaa ttctgcttgt gaagttggct atatgcacct
    23941 ttcagatggt gattttcaag atttagcctc tacaactgca ctgattgctg atgttgccaa
    24001 agcgcatgga gatgaaatta gccgatggga tggacgtcgt atttttcagg aggctaaaaa
    24061 aggcaatgaa aaatgtattg caagtataga ccgtatgata aactaccttg gccaaggaat
    24121 cgctaatatg gtttatgtgg taaatcctga aaaagttgtt ttaggtggtg gaatcatggc
    24181 tcaaaaagat tatttacaag ataaacttag tgaatctttg aaacgaaatc ttgtgacaag
    24241 tcttgctgaa aaaacagcaa tagtctttgc acaacacgaa aaccaagctg gtatgctagg
    24301 agcttactac cattttaaaa acagggggtg acgggtatat gattgaaaca atgtcgctag
    24361 atgacatgag agaataccta ggtcaagacc aaattcccga ggattttgat gacttctgga
    24421 aaaaacaaac aatgaaatat caagggaata ttgagtatcg tctagacaaa aaagatttca
    24481 atattacttt tgcacaagct tatgatcttc attttaaagg cagcaataac tctattgtct
    24541 atgccaaatg tttattccct aaaacaaata aaccctatcc tgtagttttt tattttcatg
    24601 gttaccaaaa ccaaagtcct gactggagtg accaacttaa ttatgtagca gcaggttacg
    24661 gcgttgtttc aatggatgtt cgaggtcaag ctggacagtc tcaagacaag gggcattttg
    24721 atggcattac agttaagggc cagattgtgc gaggaatgat ctcaggacca aatcatcttt
    24781 tttataaaga catttatctt gatgttttcc aattgattga tatcattgct acattagaga
    24841 gtgttgatag taatcaactt tatagttatg gctggtctca aggtggagca cttgctttga
    24901 tagctgcagc tcttaatccc aaaatcgtca agaccgttgc tgtttaccca tttttgtcag
    24961 actttagacg agtattagat ttagggggtg tcagtgagcc ctatgatgaa ctcttccgct
    25021 attttaaata tagtgatcct tttcataaaa cagagaataa tgtgttaaaa actttggctt
    25081 atattgatgt taaaaatttt gcacatagga tttcttgccc cgttgtttta ctaacagcct
    25141 taaaagatga tatttgtcct ccatcaactc aatttgccat ttttaatcgt ctgacatcaa
    25201 ctaaaaagca tcttttatta ccagattatg gacatgatcc aatgacagtg caagtaaaag
    25261 accatatttt tgatcaactg acaggaagtc aatttacaaa gcaaaaaatc gagtagatta
    25321 tttctactcg attttcattt agcgttgaat aatggtttct ctaaaaattc gctctttttc
    25381 tattttatta atatctaaaa attgtgcata gataatatct accatgatta acattgggat
    25441 ctgaggcgaa attctattgc cgtagtctaa atgcctagca gaagccacct gaataatctt
    25501 gtcaaaagca tagtcaattg tgtgaggttg ttttgtaaat agaacggttt tagctccgtg
    25561 acaacttgcg atttttaagg caccaataac agagttggtg ttcccagaaa gtgagaaggc
    25621 aatgacgagg catctatcat taacaatact attagtccat gagaaactat ctgtatcatc
    25681 cagagcctca caaatcactc ccagcctcat aaggcgtatt ttaaattctt tagctactag
    25741 cgatgagctc cctttgccat aaaaataaac acgatcagct tgctcaatca tatgtgatac
    25801 ttctagaagc tgttcttcat caataatatc agcagattta gaaatcatct cttgataatt
    25861 cataaatact ttcttagtgt tgtcattttg aagaccgtat aattgttgac tgatggtctc
    25921 atggcttttt aaatattcaa aaacaaattc acgatagcct ttaaacccac attttttagc
    25981 aaaacgcgtt aaggctgctt gtgaaatgtg tagttgctta gtgacaatgg tagaagcaag
    26041 agcgtctttt gaaatgggct gccccataaa ataacaagca atttcacgtt ctaaatcagt
    26101 cattaaatca agagcagact caatttggtc ataaatagtc ataatacacg gtcctctcct
    26161 ctttaagaaa gcgcttaata tattcagtat aacataaagt tagtgaggga ccaaactaag
    26221 ttctaagaaa cagcaatgat aagttttaaa tagcaatatg aaggatcaat aacataatat
    26281 atgttaagta tatattatga tataaatttt tcaaaaaagc tgtaataaaa aaaataaaag
    26341 cgaacaaatg tgttataatt ttataaatga tacgttttta ttgaaaattt ctttttatat
    26401 tatttaatag gagttattct gatatgaaat tgcttgttgt tggttctggt ggtcgtgagc
    26461 atgcgattgc taagaagttg ttagcgtcta aggatgtgga tcaggttttt gtggcacctg
    26521 gtaatgatgg tatgaccttg gatggtctag acttggtaaa tatcggaatt tccgaacatt
    26581 ctagactgat tgactttgtt aaggagaatg agattgcttg gacccttatt ggtcctgatg
    26641 atgcgctagc agctggtatc gttgatggtt ttaatagtgc tggactcaga gcatttggtc
    26701 caaccaaggc agccgcggag ctagagtggt caaaagactt tgccaaggaa atcatggtca
    26761 aatacaatgt tccaacagca gcctatggca cattttcaga ttttgaaaaa gctaaagcct
    26821 acatcgaaga gcagggcgca ccaatcgtgg tcaaggctga cggattggcg ttaggcaagg
    26881 gcgtggtcgt ggctgaaacc gttgagcagg cggtagaggc ggcgcaagag atgcttttgg
    26941 acaacaagtt tggcgactcg ggtgcgcgcg tggttatcga ggaattcttg gatggtgaag
    27001 agttctccct tttcgccttc gctaatggcg ataagttcta catcatgccg acagctcagg
    27061 atcacaagcg tgcctatgat ggtgacaagg ggctaaatac cggtggtatg ggtgcctatg
    27121 cgccagttcc ccacctgcct cagagcgtgg tggatacagc agttgagact atcgttaagc
    27181 ctgtccttga aggcatgatt gccgaagggc gtccttatct aggtgtcctc tatgctgggc
    27241 ttatcctgac ggctgatggc cctaaggtta tcgagttcaa ctcacgtttt ggtgaccctg
    27301 aaactcagat tatcctccct cgcctgactt ccgatttcgc tcagaacatc gacgacatca
    27361 tgatgggcat cgagccttac atcacttggc agaaggacgg cgtgactctg ggcgttgtcg
    27421 ttgcctcaga aggctatccg ctcgattacg agaaaggtgt gccactgcct gaaaagaccg
    27481 acggcgacat catcacctac tatgcgggag ctaagtttgc ggaaaatagc aaagcactgc
    27541 tctcaaacgg aggacgtgtc tacatgcttg tcaccacaga agacagcgtc aaagcagggc
    27601 aggacaaaat ctatacccaa ctcgcccaac aagacacaac aggcctcttc taccgaaacg
    27661 acatcggaag caaagctatt aaggaataag actactcatt caaatattag cgtcagcaaa
    27721 gcgaccgaag gtcgctccaa aaagaaagaa gaaaggaact gaacacggga ctaattttct
    27781 agtgaaaaag ataaatcttc ctagatgcta aagcatcttc gtcagatttc tgcgtcgtag
    27841 gcgtctgctg ctaaagcacc tagtcgccct gacgtgggta cgtcttcgaa atcaaagatt
    27901 tcggacttac cgcatatttt tctacgaaaa ttttcgacaa gtcgaaacgt ccctttgtat
    27961 cttaatatta tgcaaccaat tatttctatt atcatgggtt ccaaatccga ctggacaacc
    28021 atgcaaaaaa cggctgaagt tctagataac tttggcattg cttacgagaa gaaagtcgtc
    28081 tctgcccacc gtacgccaga cctcatgttc aagcatgctg aggaggcgcg tggtcgtggt
    28141 atcaagatta tcatcgctgg agcaggtgga gcagcccact tgccgggtat ggtagcagct
    28201 aaaacaaccc tgcctgttat cggtgtgcct gtcaaatcac gtgccctctc aggtttggat
    28261 tcgctctact ctattgtgca gatgcctggt ggtgtgcctg tggcgaccat ggctatcgga
    28321 gaagcaggag cgactaacgc tgccctgaca gccctccgta tcctctctat cgaggatcaa
    28381 aatctggctg atgcgctagc tcatttccat gaggaacaag gaaaaatcgc agaggagtca
    28441 agcaatgaac tcatttaaga ccattgggat tattggtggt ggtcagctgg ggcagatgat
    28501 ggcgattgcg gctatctaca tgggccacaa ggtcattacg ctggatccag ctagcgactg
    28561 ccctgcctcc cgcgttagcg aggtgattgt ggcaccttac gatgatgttg aggctttggg
    28621 aacattagct gcgcgttgcg atgttttgac ctatgagttt gagaatgtcg atgccgatgg
    28681 tctggatgcc gttgtgtcag ctggtcagct accgcagggg actgatctgc tccgcatttc
    28741 tcaaaaccgt atctttgaaa aagactttct ggcaaataag gctggcgtga ctgtcgctcc
    28801 ctataaggtg gtgacatcta gccttgacct agaggggctt gacttgacca agacctatgt
    28861 cctcaagaca gcgacaggtg gttatgacgg tcatgggcaa aaggttatcc gctcagcaga
    28921 agacctgcca gaggcgcagc aattagccaa ctcagctcag tgtgtcttgg aagagtttgt
    28981 caacttcgac cttgaaatat cagtcatcgt gtctggaaat ggtcaggatg tgacggtctt
    29041 tcccgttcag gaaaatatcc accgcaacaa tatcctgtca aaaaccatcg taccagctcg
    29101 catctcagac caactagctg acaaggctaa ggaaatggct gtgcagattg ccaagaaact
    29161 ccagctatca ggaaccctct gtgtggaaat gtttgcgacc gcagatgaca tcatcgtcaa
    29221 tgaaattgcc ccacgtcccc acaactcagg gcactactct atcgaagcct gcgacttttc
    29281 acagtttgac acccacatct tgggcgtact gggcgcaccg cttccgccaa tcaaactcca
    29341 tgctccagcc gttatgttca atgtcctagg acaacatgtc cagcaggcaa ttgaccatgt
    29401 tgcccaaaac cctagcgccc acctccacat gtatggtaaa ctagaagcaa aacataaccg
    29461 caaaatggga cacgtgacgg tgtttagcga tgtacctgat gaggtggaag agtttgaaga
    29521 aaggatggat ttctaaggag gtttatggaa ttttaggggg gagaatgatt ttgaagggag
    29581 tgttgtcaga tgaatagaat aataaacagg gatattttac ctagaatttc aaagatttct
    29641 aaaaataata aagagaaaga tttactatct atagcttata ttacttggct aatctttata
    29701 atatttgctt tgggagtagt tacagttaat gatttaaaac cgatgt
//
LOCUS       DATRNK010000020        28440 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.020, whole genome shotgun sequence.
ACCESSION   DATRNK010000020 DATRNK010000000
VERSION     DATRNK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28440
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.020"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(172..558)
                     /gene="rplQ"
                     /locus_tag="VBC52_000891"
     CDS             complement(172..558)
                     /gene="rplQ"
                     /locus_tag="VBC52_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEO6882488.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLIINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEASDKYTSTTALQKLFDDIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(573..1511)
                     /locus_tag="VBC52_000892"
     CDS             complement(573..1511)
                     /locus_tag="VBC52_000892"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEO6882489.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELDVEGP
                     AEITAGDILTDSDIEIVNPDHYLFTIAEGHSLKATMTVAKNRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKTEPEMMKVRNLGRKSLEEVKIKLADLGLGLKNDK"
     gene            complement(1561..1944)
                     /gene="rpsK"
                     /locus_tag="VBC52_000893"
     CDS             complement(1561..1944)
                     /gene="rpsK"
                     /locus_tag="VBC52_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEO6882490.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(1962..2327)
                     /gene="rpsM"
                     /locus_tag="VBC52_000894"
     CDS             complement(1962..2327)
                     /gene="rpsM"
                     /locus_tag="VBC52_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEO6882491.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLSTSKKILAAAGISEDIR
                     VKDLTPDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2345..2461)
                     /gene="rpmJ"
                     /locus_tag="VBC52_000895"
     CDS             complement(2345..2461)
                     /gene="rpmJ"
                     /locus_tag="VBC52_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEO6882492.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2487..2705)
                     /gene="infA"
                     /locus_tag="VBC52_000896"
     CDS             complement(2487..2705)
                     /gene="infA"
                     /locus_tag="VBC52_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEO6882493.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(2821..3459)
                     /locus_tag="VBC52_000897"
     CDS             complement(2821..3459)
                     /locus_tag="VBC52_000897"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEO6882494.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTE
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPSCLIERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPILEHYRKLGLVTDIEGNQEITEVFADVEKALLELK"
     gene            complement(3554..4858)
                     /gene="secY"
                     /locus_tag="VBC52_000898"
     CDS             complement(3554..4858)
                     /gene="secY"
                     /locus_tag="VBC52_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEO6882495.1"
                     /translation="MFLKLLRDALKVKMVRNKILFTIFILLVFRIGTHITVPGINVKS
                     LEQMGELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLFLAFVQSIGITAGFNTLSSVALVKTPNVQTYLLIGAILTTG
                     SMVVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAITTIYEDFFVNVRSSAITNSYIF
                     VGILIVAVLAIVFFTTFIQQAEYKIPIQYTKLVQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFFQNGKEIPWLTKLQELLNYQTPVGMIIYAILIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSIRPGRETEEYMSSLLKKLATIGSVFLAFISLLPIIAQQALHL
                     SSSIALGGTSLLILIATGIEGMKQLEGYLLKRRYVGFMNTTE"
     gene            complement(4879..5319)
                     /gene="rplO"
                     /locus_tag="VBC52_000899"
     CDS             complement(4879..5319)
                     /gene="rplO"
                     /locus_tag="VBC52_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEO6882496.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRMPKRGFSNINAKEYALVNLDQLNVFEDGTEVTPVVLKEAGIV
                     RAEKSGVKILGNGELTKKLSVKAAKFSKSAEAAITAKGGSIEVI"
     gene            complement(5444..5623)
                     /gene="rpmD"
                     /locus_tag="VBC52_000900"
     CDS             complement(5444..5623)
                     /gene="rpmD"
                     /locus_tag="VBC52_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEO6882497.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVNAISHLVTVEEA"
     gene            complement(5638..6132)
                     /gene="rpsE"
                     /locus_tag="VBC52_000901"
     CDS             complement(5638..6132)
                     /gene="rpsE"
                     /locus_tag="VBC52_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEO6882498.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDRNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMVEVPMVGTTIPHEVRSEFGGAKVLLKPAVEGA
                     GVAAGGAVRAVIELAGIADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6151..6507)
                     /gene="rplR"
                     /locus_tag="VBC52_000902"
     CDS             complement(6151..6507)
                     /gene="rplR"
                     /locus_tag="VBC52_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEO6882499.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNIFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSNGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADSARENGLKF"
     gene            complement(6608..7144)
                     /gene="rplF"
                     /locus_tag="VBC52_000903"
     CDS             complement(6608..7144)
                     /gene="rplF"
                     /locus_tag="VBC52_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEO6882500.1"
                     /translation="MSRIGNKVITLPAGVEIINKDNVVTVKGPKGQLTREFNKNIGIT
                     VEGTEVTVTRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKALEMRGVGYRAQLQGSK
                     LVLSVGKSHQDEVEAPEGVTFEVPTPTTINVIGINKESVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(7254..7652)
                     /gene="rpsH"
                     /locus_tag="VBC52_000904"
     CDS             complement(7254..7652)
                     /gene="rpsH"
                     /locus_tag="VBC52_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEO6882501.1"
                     /translation="MVMTDPIADFLTRIRNANQVKHEVLEVPASNIKKGIADILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGQNGERVITNLKRISKPGLRVYTKHEDMPKVLNGLG
                     IAIVSTSEGLLTDKEARQKNIGGEVLAYIW"
     gene            complement(7807..7992)
                     /locus_tag="VBC52_000905"
     CDS             complement(7807..7992)
                     /locus_tag="VBC52_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEO6882502.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCEKCGRPHSVYRKFQLCRVCF
                     RDLAYKGQVPGVTKASW"
     gene            complement(8010..8552)
                     /gene="rplE"
                     /locus_tag="VBC52_000906"
     CDS             complement(8010..8552)
                     /gene="rplE"
                     /locus_tag="VBC52_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEO6882503.1"
                     /translation="MANRLKEKYTNEVVPALTEKFNYSSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(8576..8881)
                     /gene="rplX"
                     /locus_tag="VBC52_000907"
     CDS             complement(8576..8881)
                     /gene="rplX"
                     /locus_tag="VBC52_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEO6882504.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVVVEGVALIKKH
                     QKPNNENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYNKKSGEVLD"
     gene            complement(8961..9329)
                     /gene="rplN"
                     /locus_tag="VBC52_000908"
     CDS             complement(8961..9329)
                     /gene="rplN"
                     /locus_tag="VBC52_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEO6882505.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9354..9614)
                     /gene="rpsQ"
                     /locus_tag="VBC52_000909"
     CDS             complement(9354..9614)
                     /gene="rpsQ"
                     /locus_tag="VBC52_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEO6882506.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(9640..9846)
                     /locus_tag="VBC52_000910"
     CDS             complement(9640..9846)
                     /locus_tag="VBC52_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEO6882507.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(9856..10269)
                     /gene="rplP"
                     /locus_tag="VBC52_000911"
     CDS             complement(9856..10269)
                     /gene="rplP"
                     /locus_tag="VBC52_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEO6882508.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEVAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10273..10926)
                     /gene="rpsC"
                     /locus_tag="VBC52_000912"
     CDS             complement(10273..10926)
                     /gene="rpsC"
                     /locus_tag="VBC52_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEO6882509.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(10939..11283)
                     /gene="rplV"
                     /locus_tag="VBC52_000913"
     CDS             complement(10939..11283)
                     /gene="rplV"
                     /locus_tag="VBC52_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEO6882510.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKNVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11299..11577)
                     /gene="rpsS"
                     /locus_tag="VBC52_000914"
     CDS             complement(11299..11577)
                     /gene="rpsS"
                     /locus_tag="VBC52_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEO6882511.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(11676..12509)
                     /gene="rplB"
                     /locus_tag="VBC52_000915"
     CDS             complement(11676..12509)
                     /gene="rplB"
                     /locus_tag="VBC52_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEO6882512.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTNTPEKSLLVSLKNKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YILAPKGLEVGQRIISGPEADIKVGNALPLANIPVGTVIHNIELQPGKGAELIRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATIGTVGNEQQSLVNIGKAGRNRWKGVRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNQ
                     K"
     gene            complement(12527..12823)
                     /locus_tag="VBC52_000916"
     CDS             complement(12527..12823)
                     /locus_tag="VBC52_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEO6882513.1"
                     /translation="MNLYDVIKKPVITEKSMVALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVTVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(12823..13446)
                     /gene="rplD"
                     /locus_tag="VBC52_000917"
     CDS             complement(12823..13446)
                     /gene="rplD"
                     /locus_tag="VBC52_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEO6882514.1"
                     /translation="MANVKLFDQTGKEVSSVELNEAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVENLSFAAPKTAEFASVLSALSIDSKVLVILEEGN
                     EFAALSARNLPNVTVATATTASVLDIVNADKLLVTKEAISTIEGVLA"
     gene            complement(13470..14096)
                     /gene="rplC"
                     /locus_tag="VBC52_000918"
     CDS             complement(13470..14096)
                     /gene="rplC"
                     /locus_tag="VBC52_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEO6882515.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVVLIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14201..14509)
                     /gene="rpsJ"
                     /locus_tag="VBC52_000919"
     CDS             complement(14201..14509)
                     /gene="rpsJ"
                     /locus_tag="VBC52_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEO6882516.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(14715..15995)
                     /locus_tag="VBC52_000920"
     CDS             complement(14715..15995)
                     /locus_tag="VBC52_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO6882517.1"
                     /translation="MRYSKEIIQLAIPAMIENILQMLMGVVDNYLVAQLGVVAVSGVS
                     VANNIITIYQAIFIALGASIASLLAKSLAGSKKDDAISVCSQAIFLTLLIGAVLGIIS
                     IVFGQTFFKLLGTTKSVAQVGGLYLAIVGGGVVTLGMLTTLGSFLRVQGQPRLPMYVS
                     IFVNFLNAVLSGFAIFEWRYGLVGVAVSTLIARLIGICILAKYLPIKKIIKRMTWKIS
                     AQIWNLALPSAGERLMMRAGDVVIVAIVVQLGTNVVAGNAIGETLTQFNYMPGLGIAT
                     ATIILTAKYVGQKNRESIEETIQSSYYIGLVLMILISSFMLLAGKPLTQLFTNNPSAI
                     KGSLIVILLSFVGVPATIGTLVYTAAWQGLGNAKLPFYTTTIGMWLIRVVLGYLLGIV
                     FELGLLGVWMATIADNIFRWLFLKVHYHRYIQKM"
     gene            complement(16100..17590)
                     /locus_tag="VBC52_000921"
     CDS             complement(16100..17590)
                     /locus_tag="VBC52_000921"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEO6882518.1"
                     /translation="MAIVYQSTRDASNKVTASQAILKGLADDGGLFTPITFPKVDLDF
                     TKLKDASYQEVAKLVLSAFFDDFTEQELDYCISQAYDTKFDTSEIAPIVKIGDRYHLE
                     LFHGPTIAFKDMALSILPYLLTTAAKKQGVDNKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKNGVSYIQELQMITQAGQNTHVVAIEGNFDDAQTSVKEMFNNSLLRLKLSQ
                     QHMQLSSANSMNIGRLVPQIVYYIYAYAQLVKSKEISIGQPINFSVPTGNFGNILAAY
                     YASQIGLPVTKLICASNDNNVLTDFFKTQTYDKNREFKVTSSPSMDILVSSNLERLIF
                     HLLGDDAETTKKLMEDLVTTGEYALEARQANILESFVAGFATEQFVELDIKHLFDQYQ
                     YIEDPHTAVASAVYQAYQTETKDQTPAVIVSTASPYKFPCVVTKAITNKEEIQDFAAI
                     SILNDLSGVSLPKAVTDLQKAEVIHRTVVPTSNMRETVEECLGLKQ"
     gene            complement(17710..18726)
                     /gene="adhP"
                     /locus_tag="VBC52_000922"
     CDS             complement(17710..18726)
                     /gene="adhP"
                     /locus_tag="VBC52_000922"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEO6882519.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPKVGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLRVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAIVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIAVYGAGGLGNLAVQYAKKVFNAHVVAVDINADKLQLAKEVGADLTVNGKEIK
                     DVAAYIQEKTGGCHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIRVVGSLVGTRKDLEEAFAFGAEGLVVPVVEKVPVDTAPQVFDEMERGLIQGRKVL
                     DFTK"
     gene            complement(18905..21547)
                     /gene="adhE"
                     /locus_tag="VBC52_000923"
                     /gene_synonym="adhC"
     CDS             complement(18905..21547)
                     /gene="adhE"
                     /locus_tag="VBC52_000923"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEO6882520.1"
                     /translation="MTEKTKAVETTDVALAIDTLVQNGLKALDEMRQLNQEQVDYIVA
                     KASVAALDAHGELALHAVEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEEDDV
                     TGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWIEQPSIDATNALMNHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYKEFVEEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKSAVWIAEQAGFTVPEGTN
                     ILAAECTEVSEKEPLTREKLSPVIAVLKAESTEDGVEKARQMVEFNGLGHSAAIHTKD
                     ADLAREFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTDLMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVMTVPGFIAADTGMDVLTHATEAYVSQMANDY
                     TDGLALQAIKIVFDYLERSVKDADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPAATP
                     EEAVESYAKAVYDLGTRLGIKMNFRDQGIDEKEWKEKSRELAFLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYEERPGRRK"
     gene            complement(21817..23595)
                     /locus_tag="VBC52_000924"
     CDS             complement(21817..23595)
                     /locus_tag="VBC52_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO6882521.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFKNSFPGGFVGVDIFFTFSGFL
                     ITALLIDEFSKTKKIDFVSFCRRRFYRIFPPLVLMVLVTIPFVFLVKSDFRASIGSQI
                     MTALGFTSNFYEILTGGNYESQFIPHLFVHTWSLSIEVHFYVLWGLTVWLLSKRSKDQ
                     KQLRGTLFLISMGIFGVSFLTMFVRAFFVDNFSTIYFSTLSHIFPFFLGAMVATISGI
                     REITGRFKKNIKNLTLKHNLIMMGSAFAGLMILTFALDFDNRLTYLFGFVLSSIFASV
                     MIYNARILHEHTPDISEPFVITYLADISYGMYLFHWPFYIIFSRLSPNWIAVILTVVL
                     SAVFSTLSFYIIEPFILGRKPKFLDYEFDLLPYKKWLFSIGGVLTLITVVTMLTAPSI
                     GSFETELLQNSLQQARTNTNRTHTLAAGDAGALSDVTVIGDSVALRSSAAFNKLLPEV
                     QLDAAVSRNFSKSFDIFENRIQNKALSKIVVLAVGVNSLDNYKTDLSQFIKSLPKGHR
                     LIIVTPYNAKNMSQVTTVRDYELSLMKKYNYITVADWYKVATEHPEIWGNTDGVHYSD
                     SDTTGADLYVSNVKKAIQKSAQRAAK"
     gene            complement(23592..23972)
                     /locus_tag="VBC52_000925"
     CDS             complement(23592..23972)
                     /locus_tag="VBC52_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882522.1"
                     /translation="MKNFKITRTHLEILSLIIIVVFGLSVFTLTTSSQGVFSYDGGKI
                     KYVGSIVNHHMTGKGKLTYENGDYYKGDFVNGVFEGKGTFVSVHGWSYTGDFKKGQPD
                     GQGRLNAKNKKVYKGTFKQGIYQK"
     gene            complement(23979..24416)
                     /locus_tag="VBC52_000926"
     CDS             complement(23979..24416)
                     /locus_tag="VBC52_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEO6882523.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMANLDKEDSLLVESRATSTWEHG
                     NPIHRGTQAILQRYQIPYINDKGSQPISKDDLAYFDYIIGMDEQNVKDLKALSQGTYD
                     DKISLFCDGGVPDPWYTGDFEETYRLVNDGCKQWLTLINNSRD"
     gene            complement(24568..25566)
                     /gene="ruvB"
                     /locus_tag="VBC52_000927"
     CDS             complement(24568..25566)
                     /gene="ruvB"
                     /locus_tag="VBC52_000927"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001902090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEO6882524.1"
                     /translation="MTRFLDSDAMGDEELVERTLRPQYLREYIGQDKVKDQLKIFIEA
                     AKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAIEKSGDLVAILNDLE
                     PGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGETSRSVHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYEENDLTEIIERTADIFEMKITYEAASELARRSRGTPR
                     IANRLLKRVRDYAQIMGDGLIDDNITDKALTMLDVDHEGLDYVDQKILRTMIEMYNGG
                     PVGLGTLSVNIAEERDTVEDMYEPYLIQKGFIMRTRTGRVATDKAYEHLGYQRFDK"
     gene            complement(25863..26774)
                     /locus_tag="VBC52_000928"
     CDS             complement(25863..26774)
                     /locus_tag="VBC52_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6882525.1"
                     /translation="MLKHFGSKVRNLRVTRNITREDFCGDETELSVRQLARIESGQSI
                     PNLTKAHYIAKQLNVKLDILTGGESLELPKRYKELKYLILRIPTYADAERLKLRECQF
                     DHIFEEFYDNLPEDECLAIDSLQAKFEVYQTGDINFGVEVLCECFDKVKYKEKYTLND
                     LIIIDLFLTCAVVSKFNNRAFTKEVFQTICKTLISQNHKLTAEDLFWFNHVLLNCVFV
                     GLCLNSEECLAEMLEVSRQTMVSTHDFHKMPLYFMYQWKYFITIDNDIKSAENAYQQS
                     IMFSKMIDDKHLIKKLELEWQEDITGH"
     gene            complement(26926..28224)
                     /gene="purB"
                     /locus_tag="VBC52_000929"
     CDS             complement(26926..28224)
                     /gene="purB"
                     /locus_tag="VBC52_000929"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEO6882526.1"
                     /translation="MIERYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEQYVCDKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDTKVTSCLTQEEIDELFNPIYYTKRVDDIFERLGLEK"
     gene            complement(28249..>28440)
                     /locus_tag="VBC52_000930"
     CDS             complement(28249..>28440)
                     /locus_tag="VBC52_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882527.1"
                     /translation="ESIFDHNNQKIFIPKLYEEYIYILISLGEVEKAKEILKEVSDYL
                     TEESLNYFEKEYDKAKGSN"
BASE COUNT         8551 a         5906 c         4812 g         9171 t
ORIGIN      
        1 acgcctgctt acgaaatata aacaaaattg tttgcatttc gtaaacaagc gttacctatt
       61 taacaatata tgatgagtgt tcccgctgag aataattctc agcggtagac cagagctaga
      121 ctaagaatcg attgattcca tcatcataac actcaacaaa attgataaaa attatactaa
      181 ttcaataatt gccattgggg cagcatcgcc acggcgtggt tctgttttaa gaatacgagt
      241 gtatcctccg ttacgttcag cataacgagg tgcgatgtcg tcaaaaagtt tttgaagagc
      301 tgttgttgat gtatatttat cagaagcttc atcatagttt tctgatgcaa tttcattacg
      361 tacataagca gcagcttgac gacgagcatg taaatcacca cgtttaccta gagtaatcat
      421 tttttcaact gttttacgga tttctttagc acgtgcttca gtagttacaa ttgattcgtt
      481 gataataaga tcggttgtca aatcacgaag cattgcctta cgttgtgagc tagtgcgtcc
      541 tagtttacgg taagccatta tgtcctccta ttttatttat cgttttttaa tccaagacct
      601 agatcggcaa gtttgatttt aacttcttca agactcttac gtcctaagtt tcggactttc
      661 atcatttcag gctcagtttt ttctgttaaa tcaaatactg tattaattcc agcacgtttt
      721 aaacagttat atgagcgcac tgacaaatca agttcctcaa ttgtccggtc aagcactttc
      781 tcatcgttca ctttctctgt ttccttcatt acttcagttg ctttagcaac ctctgttaaa
      841 tcagtaaaca agtttaagtg ttcaattaag acgcgagctg aaagaccaag agcatcctca
      901 ggaatgattg tgccatttgt catgatttca atagttaatt tatcaaagcc atcgttacta
      961 cctacacgag caggttcaac ttgataatta acttttttca ctggtgtata gattgaatct
     1021 acagccaatg ttcccactgg tgcatcatct tttttatttc cttctgctgg aacataacct
     1081 cggtttttag caactgtcat tgttgctttc aaactgtgac cttcagcaat tgtgaaaaga
     1141 taatggtcag ggtttacaat ttcgatatca ctatctgtta aaatatctcc ggcagtaatt
     1201 tctgcaggac cttcaacgtc tagttcgata atcttttcgt cttcgacgta agatttcaca
     1261 gcaagtcctt ttacattaag gatgatttgc atcacatctt cacgtacacc tgggatagta
     1321 tcaaattcgt gtaatactcc atcaattttg attgatgtga ctgctgcacc tggaagtgaa
     1381 gacagaagca cacgacgaag agagtttcct agagttgtac cgtagccacg ttctagtggt
     1441 tcgatgacaa atctgccgta atctttattt tcatcaattt ttgttattat tggtttttca
     1501 aactcaatca tttagttact ccctcttaaa cgaaaagttg tgtactataa aatgctatga
     1561 ttatacacga cgacgttttg gaggacgagc accattatgt ggtacaggag tcacatcacg
     1621 aattgcagtt acttcaagac cagctgcagc aagggcacgg atagcagatt cacgacctga
     1681 accaggacct tttacagtaa cttcaacagt tttaagaccg tgttcttgtg cagatttagc
     1741 agcagcttca gcagccattt gagcagcaaa tggagtagat ttacgagaac ctttaaatcc
     1801 aagagcacca gctgatgacc atgcaagagc gttaccatgc acatctgtaa tcataacaat
     1861 agtgttattg aatgtagcgt gaatatgagc aacaccagat tcgatgttct ttttcacacg
     1921 acgtttgcgt gttggtttag ccaattgttt tacctcctat tttatttttt cttacctgca
     1981 atcgcaacag ctttaccttt acgagtgcga gcgttatttt tagtattttg tccacggaca
     2041 ggaagtccac gacggtgacg gattccacgg tatgaaccga tttccatcaa acgtttaata
     2101 ttcaagttta cctcacgacg taggtcacct tcaactttga ttgcatcaac ttcacgacgg
     2161 atagcgtcct cttgatctgg tgtcaaatct ttaacgcgga tatcttcaga aatacctgca
     2221 gctgctaaga tttttttaga tgtagaaaga ccaattccgt atacataagt aagtgaaatt
     2281 actacacgtt tgtcatttgg aatatcaact ccagcaatac gagccatttt atctcctttc
     2341 tatattatcc ttgacgttgt ttgtgttttg ggtttgtcgg acaaattacc ataacacgac
     2401 cgttacgacg aataacttta cagtattcgc aaattggttt aaccgatggt cttaccttca
     2461 tgttttaatc cctccaatta tttcgactat ttaaagcggt atgtgatgcg tccacgtgtt
     2521 aaatcgtatg ggctcatttc aacagtaaca cgatcaccta ccaaaatacg gatgtagttt
     2581 ttacggattt ttcctgatac agttgccaaa atttggtgtc cattttccaa ctcaacagta
     2641 aacattgcgt taggcatagt ttcaacaact ttaccttcaa tttcaatcac atcttctttt
     2701 gccacgcaaa agtaccccct aaatttcgat ttgatgtcct ctataaagag gtaacaatta
     2761 taagtcagac taacttagta tatcattact aatctactta tgcaagtaga ttagcataat
     2821 ttatttgagt tctagcaacg ctttttcaac atctgcaaaa acttctgtta tttcttgatt
     2881 accttcaata tctgtaacaa gaccaagctt acgatagtgt tcaagaatag gttctccttg
     2941 agcaatatta acgtccaagc gacgtttgac agtttcaggc ttatcatctt cacgttgata
     3001 gtaatcttct tctttataat ctactggtgg gttgaacact ttgtggaaag tttcaccagt
     3061 tttacgattg ataatacgac cactcaaacg ctctataaga catgatggat ccactttaat
     3121 attaataaca ccatctaagc gtagtcctag ttcttcaagc gtagcatcta aggcgtgtgc
     3181 ttgttcaata gtacgtggat atccatcaag taaaaaacct ttttctgcga tatcatcctc
     3241 agctaagcgc tcttttacaa tcccgtttgt tacttcatca ggaaccaatt cacctttatc
     3301 aatataactt ttagctaaac gtcccatttc ggtttgatta gccattgcgg cgcggaacat
     3361 atcccctgtt gagatgtgag caacaccaaa ttcttcaacg atcttagctg cttgagtacc
     3421 tttaccagca ccaggcaaac ccataattaa aagattcatt atagtctcct tattttgttt
     3481 ttaaataaat ccaacacagc atgtcggact tattaaaaaa caaaatagaa ggttgacaga
     3541 gtcaacctat attctattct gttgtattca tgaatccaac atagcgtctc ttcaaaagat
     3601 agccctcgag ttgtttcata ccttcaatac ccgttgcaat caaaatgagt aagcttgtcc
     3661 cacctaacgc aatacttgat gacaaatgca acgcttgttg agcaataatt ggtaacaagg
     3721 aaataaatgc tagaaatact gaaccaattg ttgctaattt tttcaatagc gatgacatat
     3781 attcttctgt ttctcgtcct ggtcgaatac ttggaatgta ggacgaattt ttttgaagat
     3841 tctctgctgt cttttcaggg tttacttgta caaaagtata gaagaaagag aacagaataa
     3901 tcaagatagc atagataatc ataccgactg gggtttgata attaagtaat tcttggagtt
     3961 tagtaagcca aggaatttct ttgccatttt gaaagaaagg aataatcgta cttggaattg
     4021 ttgtaatcga gctagcaaag ataacgggaa taacaccagc tggatttact tttagaggaa
     4081 gatatgaact tgtaggagct ccttgaacaa gttttgtata ttggattgga attttgtatt
     4141 cagcttgttg aataaatgtt gtaaaaaata caattgctaa tactgctacg ataaggatcc
     4201 cgacaaagat gtaagaattt gtaattgctg aagagcgtac atttacaaag aaatcttcgt
     4261 agatagttgt aattgcactt ggaatggaag aaatgatacc cgcaaagata atcatagaca
     4321 ctccatttcc gaatccctta tcagtaattt gttcacctag ccaagtaacc accatactac
     4381 ctgttgtaag gattgcacca ataagcaaat atgtttgtac attcggagtt ttaactaaag
     4441 caacacttga tagagtgtta aaaccagcag taataccaat tgattgtacg aatgcaagga
     4501 acaaagaaat ataacgtgta gcttggttaa gcttacgacg acctacctct ccttgttttc
     4561 cccattcaac aaacttcgga agaatatcca tttgaagtaa ctgaacaaca atagacgcag
     4621 taatgtaagg gctaactccc atagagaata ccgaaaagtt tctcattgca ttaccactaa
     4681 ctaagttaag catatttaga aatggaagtt cacccatttg ttctaaactc ttaacattga
     4741 tgcccggtac tgtgatatgc gttccgatac gaaatacaag aaggataaag atagtaaata
     4801 aaattttatt tcttaccatc tttactttga gtgcatctct aagtaatttt aaaaacataa
     4861 tgagttacct ctcattaatt agatgacttc gattgaacca cctttagcag tgatagctgc
     4921 ttcagcagat ttagaaaatt tagctgcttt aacagacaat ttcttagtta attcaccatt
     4981 accaagaatt ttaacgcctg atttttcagc acgcacgatt ccagcttctt taagaacaac
     5041 tggagttact tcagtaccat cttcaaagac atttaattgg tcaaggttca caagagcata
     5101 ttcttttgca ttgatgtttg agaacccacg ttttggcata cgacggaaca atggagtttg
     5161 tccaccttca aaaccaaggc gtactccacc accactacgc gctttttgtc ctttttgacc
     5221 acgtccagaa gttttaccat tacctgaaga tgtaccgcga cccacacgat tgcggacttt
     5281 acgtgatcct tctgcaggtt ttaattcatg aagtttcatt attttttctc cttatttgta
     5341 aaatgctaac gcctttacga gggaaaaggt actccccgca aagactcgcc tatacgattt
     5401 gtttaaatcg caccaataac aatcagtgcg atttagcatt taattaagct tcttcaacag
     5461 ttactaagtg agagatagcg ttaaccatac cacggatggc agcattatct tccttaacaa
     5521 ctgaagagtt taatttacca agtccaaggg caacaactgt tttacgttgt tctggtttgc
     5581 gaccgattgg agacttagtc aaagtaattt taatttgagc cattataatc ccctttctta
     5641 tgctaagtca gaaacagaaa tgccacgtaa agcagcaact tcttctgcac gttttaattg
     5701 tttcaagccc tcaacagttg cgcgaacaat gttgattgga gtgtttgaac caagtgattt
     5761 agatgtaata tctgcaatac ctgctaattc gatgacggca cgaacagcac ccccagcggc
     5821 aaccccagca ccctcaacag ctggtttcaa caatactttc gctccaccaa attctgaacg
     5881 aacttcgtga ggaattgttg taccgaccat aggaacttca accatgtttt tcttagcagc
     5941 ttcaacagct ttgcgaatag cttctggtac ttcttgagct ttacctgtac caaaaccaac
     6001 acgaccatta cggtcaccaa caactacaag agctgcaaaa cgtagacgac gtccaccttt
     6061 aacaactttt gtaacgcggt taatcgcaac aacgcgctct tcaagttcta ctgcattatc
     6121 tttaaatgcc attattaagt gtcctcccta ttagaatttc aatccgtttt cacgagctga
     6181 gtcagccaaa gcttttacac gtccgtgata gagatatcca ccgcggtcaa acaccacttc
     6241 agaaatacct ttagctactg cgcgttcagc aacaagttta ccgacaacaa cggcttgttc
     6301 tgtttttgtt ccgttagaaa cttctttatc aagagttgat gcacttgcga gcgttacacc
     6361 cgctacgtca tcaattactt gagcgtagat gcctgtatta gaacggaaaa tgttcaaacg
     6421 tgggcgatca gcagttccag agagtttacc gcgaacgcga cggtggcgtt tttggcggat
     6481 tttattttta tctggtttcg aaatcacaat tttcacctct taaatatatt gttatgtcaa
     6541 aatgacaata ttaaaatgaa aagatagaga atttgcagaa aagattctac aaaatctatc
     6601 aatcctatta tttacctgtt ttaccttctt tacggcgtac gaattcacca acgtaacgaa
     6661 tacctttacc tttatatggt tctggtgaac gtaagctacg aacgtaagca gcagtttgtc
     6721 caacgctttc tttgttgata ccgattacgt tgattgttgt tggagttggc acttcaaaag
     6781 taacaccttc tggagcttca acttcgtctt ggtgagactt accaacagaa agaacaagtt
     6841 ttgatccttg aagttgtgca cggtaaccaa caccgcgcat ttcaagagct tttttgaaac
     6901 cttcagaaac accaacaacc atgttgttca agttcgcacg agttgtacca tgaattgttt
     6961 tcatttcttt tgagtcgtta ggacgagtaa ctgttacttc agtcccttca acagtgattc
     7021 caatattttt attaaactca cgagtgagtt ggcctttagg tccttttaca gtaacaacat
     7081 tatccttgtt aatgatttca acacctgcag gcaatgtaat tactttatta ccaatacgtg
     7141 acatgtttat tttctcctgt taaattgtca agctgcttga gcaactagtt ttcacgggga
     7201 ctcatgagaa cacaggatta tactgtgact caatgagcat tttaggtaaa attttaccaa
     7261 atatatgcaa gtacttctcc accaatattt ttttggcgag cttctttgtc agtcaaaaga
     7321 ccttcagaag ttgatacaat tgcaatacca agtccgttaa gaactttagg catatcttca
     7381 tgtttagtat aaacacgaag acctggtttt gaaatacgtt tcaagttagt gataacacgt
     7441 tcaccatttt gtccatattt aaggaacaca cggatgatgc cttgtttgtc atcttcaata
     7501 acttcaacgt tttttacaaa accttcacgt ttaaggatat cagcaatccc ttttttaatg
     7561 tttgatgcag gtacttctaa cacttcatgt tttacttggt tagcattacg aatacgagtt
     7621 aaaaagtctg caattgggtc agtcataacc attttttaat tctcctctta ttagcagttt
     7681 gaattgaatc acttgctaat taatgagtcc ttacggacgt tgtaagacta ggtcttagta
     7741 tatttgcgtt taaaaatcag tgccattatc aacgacacct tttaaacaca aagcatcatt
     7801 aatttattac caagatgctt tagttacgcc tggtacttga cctttataag ccaagtcacg
     7861 gaagcaaaca cggcaaagtt ggaatttgcg gtaaactgag tgtggacgtc cgcatttttc
     7921 acaacgagtg taagcttgcg tagagaactt cgctgggcgt ttgttcttag caatcataga
     7981 ttttttagcc aatttattta cctcctagat tactttgcaa aaggcattcc aaggcctttt
     8041 agcaattcac gtgactcttc atcagtgtta gcagtagtaa cgataacgat atcaagacca
     8101 cgtactttat caacgtcatc aaagttgatt tctgggaaga tcaattgttc tttaactcca
     8161 agagtgtagt ttccacgtcc atcaaatgat ttcgttggaa caccatggaa gtcacgtaca
     8221 cgtggaagtg aaacagaaac taatttatcc aagaattcgt acatacgttc accgcgcagg
     8281 gtaacttttg caccgatcgc tacaccttca cgaagacgga agccagcgat tgatttctta
     8341 gctttagtaa taagtggttt ttgacctgaa ataagtgcta actcagcagc agctttctca
     8401 agatttttag cgtttgatac agcatcacca acacccatgt taagaacgat tttctcaact
     8461 tttggcacag ccataacaga tgaatagtta aatttttctg tcaacgcagg aacaacttca
     8521 ttagtatatt tttcttttaa gcgatttgcc attatgcttc tcctttcctt cgtgattaat
     8581 caagcacttc gcctgatttt ttgttgtaac gaactttttt gccgtcaaca actttgtaac
     8641 caacacgtcc tgctacacca tttttatcaa gaacttgaac gtttgacaca tggattggag
     8701 cttctttttc aacgatagcg ccttgagggt tttcgttatt tggtttttga tgtttcttga
     8761 tgagggctac gccttcaaca acaactttgt ttacttttgg aagagcttta agaactacag
     8821 cttcagtgcc tttgtcctta ccagcaataa cgcgaacttt gtcgcctttt tttacaaaca
     8881 tttgagtttt tctcctattt ttcttacgcc tcgaaaggca ccctaggaaa tcctagggga
     8941 ctacgtttgt taatctgaga ttaaagtact tctggtgcca atgaaacgat cttcatgtag
     9001 ccaccttcac gcaattcacg tgcaactggg ccaaagatac gagttccgcg aggagtttta
     9061 tcgtcacgga tgattacagc agcattgtcg tcaaatttga tgtatgaacc gtctgggcgg
     9121 cgtgcaccag tttttgtacg aacgataaca gctttaacca catcaccttt tttaactgct
     9181 ccaccaggag tagcttgttt tacagaagca acgattacgt caccgatgtt agcgaattta
     9241 cgtcctgaac caccaagtac tttgatagtc aagatctcac gagcaccgct attatcagca
     9301 actttcaagc gagtttcttg ttgaatcatt tcaattttct ccttttagtt tgattagata
     9361 ataacagctt tttccactac ttccacaaga cggaaacgtt ttgtagctga aagtgggcga
     9421 gtttccatga tacgaacgat atcgccttct ttagcaacgt tgttttcatc atgtgcttta
     9481 tattttttag aatagttgat acgtttacca tagactgggt ggttacgttt agtttcaact
     9541 acaactgtga ttgttttgtc catcttgtca gacactacgc gtccgtaaag agttttacgt
     9601 tgattacgtt ccatttagaa tttctccttt cccaatctat tatttcattt ctgattgcac
     9661 agttttaaca cgtgcaattt gtttcttaac ttcgtcaagg cgagcagtct tttcaagttg
     9721 acctgctgca gcttggaaac gaagatcaaa caattctttt ttgagttcgt tttctttctt
     9781 agcaagttct tcttgagaca atccacgaag ctctttaaca aaatctttaa tttcttgaag
     9841 tttcatgtct tctccttatt ctgcttcacg ttttacgaat ttgcatttaa ctggtaattt
     9901 gtggctagca agacgtaatg cttcacgtgc aacttcttca gaaacaccag caatttcaaa
     9961 catcacttta ccacgtttaa ctggtgctac ccaaccttca ggtgcccctt taccagaacc
    10021 catacgtaca ccgatagctt ttgcagtgta tgatttgtgt gggaagattt taatccaaac
    10081 tttaccacca cgtttcatgt aacgagtcat cgcaatacgg gcagcttcga tttgacggtt
    10141 tgtaatccag tggctagttg tagcttgaag tccgtattca ccgaatgaaa cttctttacc
    10201 acctttagcc tcaccgcgca tttttccacg gaattcgcga cggtgtttaa cacgtttagg
    10261 tactaacatt tgttatttgc ctcctttagt gtttttacga gctggaagaa cttctccacg
    10321 gtaaatccaa actttaacgc caagtttacc ataagttgtg tcagcttctt cccaagcata
    10381 gtcgatatca gcacgaagcg tgtgaagcgg aacagttcct tctgaataac cttcagcacg
    10441 agcgatatca gcaccgttca aacgaccaga aacttgagtt ttaatacctt tagctcctgc
    10501 acgcattgta cgttggattg cttgtttttg agcacgacgg aaagcaacac gttgctctaa
    10561 ttgacgagca atattttcac caacaaggtg agcatctaaa tcaggttgtt tgatttcaat
    10621 gatgttgatg tgtacttgtt ttccagttaa tttgttaagt tgaccacgaa gagcgtcaac
    10681 gtttgctcca cctttaccga taaccatacc tggtttagca gtgtgaagtg aaacaataac
    10741 tttgtttact gcacgttcaa tttcaatagt tgaaactgat gcgtcagcta attctttatt
    10801 aatgaattta cggattgcaa gatcttcatg aaggtaatcc gcgtattctt tttcagcata
    10861 ccatttcgca tcccagtcac ggatgattcc gacacgcata ccaattggat gtactttttg
    10921 acccacgatt ttacctcctt atttttctga tacaactaca gttacgtgag ttgtacgttt
    10981 gttgattggt gaagctgaac ctttcgcacg tggacggaaa cgtttcattg ttggtccttc
    11041 gttagcgaat gtttcagata ctaccaagtt tgctttttcc aaaccaaagt tgttttctgc
    11101 atttgcaatt gctgagttaa gagttttctc aataacacga gctgctttgt ttggagtgaa
    11161 ttttaagatt gcgattgcgt cagcaacatt cttaccacgg ataagatcaa gtactaaacg
    11221 tgttttacga ggtgaaacac gcactgtacg agccattgct ttagctgaag taatttctgc
    11281 cattgtgtcc tcctcctatt aacgacgtgt tttcttgtcg tcagctgcgt gacctttgta
    11341 agtacgtgtt ggtgcaaatt caccaagttt gtgacctacc atgtcctctt ggatataaac
    11401 aggaacatgt ttacgtccat cataaactgc gattgtgtat ccgatgaaac ttgggaaaat
    11461 cgttgaacga cgtgaccaag ttttgattac ttttttcttt tcgtcatttg cttgagcttc
    11521 aacttttttc attaaatgct cgtcgacgaa aggtcctttt ttaagactac gtcccatttt
    11581 gtagttttct cctttaaata atgtaccaca acggcctgta cactatagtg tactaccgag
    11641 ctggcggatt tgtataagat tatgctatta acctattatt tttggttacg acgacgaaca
    11701 ataagtttgt ctgatttagc tttcttatta cgagttttaa gtccaagcgc aggtttaccc
    11761 catggagtag atggtgcttt acgaccaact ggtgctttac cttcaccacc accgtgtggg
    11821 tgatcgttag ggttcattac agaaccacga actgtagggc gaacaccttt ccaacgatta
    11881 cgtcctgctt taccaatgtt aacaagtgat tgctgttcat taccaacagt accaattgtt
    11941 gcacggcaag taccaaggat catacgtact tcacctgatt gaaggcgaac aagaacatat
    12001 ttaccttctt gaccaagtac ttgagcagaa gcaccagcgg cacgaatcaa ttcagcacct
    12061 ttacctggtt gcaattcaat attatggata actgtaccga ctggaatgtt agcaagcgga
    12121 agtgcgttac caactttgat gtctgcttct ggaccagaaa taatgcgttg acctacttca
    12181 aggcctttag gcgcaaggat gtaagcttta acaccatcag tatagtgtac aagagcaata
    12241 tttgcagtac gatttggatc gtattcaatt gttttaacaa ctgcttcaac accatcttta
    12301 ttacgtttga agtcaatcaa acggtaatga cgtttgtgtc caccaccttg gtgacgaaca
    12361 gtaatacgtc cgttgttgtt acgtcctgct ttattcttta gtgaaacaag caatgatttc
    12421 tcaggagtgt ttgtagtgat ttctgcaaaa tccaaagaag tcatattacg acggccattt
    12481 gtcgttggtt tataaacttt aatacccacg ttatttcctc cttagattat tcagcttcag
    12541 cagcaaacaa ctcgattgct tttgaatcag ctgtaagagt gataatagct tttttagttt
    12601 ttgaagtgaa acctgtgtaa cgaccaacac gttttgcttt gggtttaact gtcacagtgt
    12661 ttacacttgc aacttttact ccgtcaaatg cagcttcaac agcttgtttg atcaaaagtt
    12721 tgtgtgcacg tgtgtcaact tcaaatgtat atttccctgc ttcaagagca accattgatt
    12781 tctcagtgat aacaggtttt ttgattacgt catacaaatt cattatgcaa gaaccccctc
    12841 gattgtagag attgcttctt tagtaacaag aagtttgtct gcgttaacga tatcaagaac
    12901 acttgcagta gttgcagtag caactgtaac gtttggaagg ttacgcgctg aaagtgcagc
    12961 aaattcgtta ccttcttcaa ggattacaag tacttttgaa tcaatgctaa gtgctgaaag
    13021 aactgacgca aattcagcag tttttggagc tgcaaatgaa aggttttcta cagctacaaa
    13081 tttatcttca gcaacttttg ctgaataaac tgatttcaac gcaaggcgac gaactttttg
    13141 tggaagtttg tagccgtatg aacgtggagt tggtccaaag acaacaccac caccacgcca
    13201 ttgtggtgag cggatagaac cttgacgagc gcgtccagtt cctttttgac gccatggttt
    13261 acgtccacca ccagatactg ctgaacggtt tttaaccgcg tgagttcctt ggcgaaggct
    13321 agcgcgttgg ctgattacaa catcaaaaac aactgattcg tttggttcaa taccgaagat
    13381 agcttcgtta agctcaactg agctaacttc tttaccagtt tggtcaaata gtttaacgtt
    13441 tgccatttta actgttttct cctttcttat tatttagcag ctttaactgc tgatttgata
    13501 gtgataagag atttcttagc acctggtacg ttacctttga tgaggacaac gtttttctct
    13561 gggataactt gtacaatttc aaggttttga actgttacac ggttaccacc catacgtcct
    13621 gccaagcgtt tgtttttgaa aacgcggtta ggcgcaacag gtcccataga acctggacga
    13681 cgatggtaac gagaaccgtg agccatagga ccacgtgatt gaccatggcg tttgataaca
    13741 ccttggaaac ctttaccttt tgatgtccct gtgacgtcaa caacatcacc agcttcaaat
    13801 tgttctacag ataattctgc accaacttct aagccttcaa tgtttttgaa ttcacgaatg
    13861 aagcgcttag gagctgtgtt tgcttttgca acatggcctt tggcaggttt gttactcaat
    13921 acttcacgtt tgtcatcaaa accaacctga actgcttcgt aaccgtctgt ttcaacagtt
    13981 ttaacttgaa gcacaacgtt tggagttgct tcaatgacag taacagggat gaattcgcct
    14041 gattcagtga agatttgagt cattcccact tttttcccta agattccttt tgtcatgaga
    14101 aaatatttcc ttttcttatt ttatttcaaa aagtttttta tgagcgtttt ttgtgctcaa
    14161 aaaagttttt tacgagcgtt ttttgtgctc agaaatcaaa ttaaagtttg atttctacgt
    14221 tgacaccact tggaagatca agtttcatca aagcatcaac agttttttgt gttgggttga
    14281 tgatgtctac caaacgtttg tgtgtacgca tttcaaactg ttcgcgagaa tctttatatt
    14341 tgtgagtcgc acgaataatt gtgtaaagac tgcgttcagt tggaagcgga actggtccag
    14401 caactgtagc acctgtacgt gttgcagttt caacgatttt ttctgccgct gtatcaagtg
    14461 tacggtgttc gtacgctttc aaacggatac ggattttttt gtttgccatc ttttctcctc
    14521 tcgtctattt acgataatag gctagctcct caagaaaacc aacacgagtc gcgtggcaat
    14581 gcaacctagc gtgtcgcaac ctcttgcatc atagctaaag ctgttcatac acagcaccag
    14641 tatatgatat caaaaaaaaa cagcacacgc aagcattatt ttattatttt cgaaaaaaat
    14701 gcaaccattt ctggttacat tttttgaata tatcgatgat agtgaacttt caaaaacagc
    14761 cacctaaaga tattgtctgc tatcgtagcc atccaaaccc caagcaaacc taattcaaaa
    14821 actattccta ataaataacc aagaactact ctgattaacc acatgcctat agtcgttgtg
    14881 taaaatggga gtttagcatt tcctaatcct tgccaagctg ctgtataaac caaggtccca
    14941 atagtagcag gtactcctac gaaagatagc aaaataacaa tcaaacttcc tttaatggca
    15001 gaaggattat ttgtaaataa ctgtgtcaat ggtttgcctg caagaagcat aaaacttgat
    15061 ataaggatca ttagaactaa gccaatataa tacgaacttt gaatagtttc ttctatagat
    15121 tctctgtttt tttgtcctac atattttgca gttaggataa ttgtagcagt tgcgatgcca
    15181 agtcctggca tataattaaa ttgtgtcaag gtttctccaa tagcatttcc cgctacaaca
    15241 tttgtaccta actgaacaac aatagcaact ataacaacat ctccagctcg catcatcaga
    15301 cgttctcctg ctgatggtaa ggcaagattc caaatttgtg cactgatttt ccaagtcatt
    15361 ctttttatta tctttttaat aggaagatac ttcgcaagga tacaaatacc aattaatctt
    15421 gcgataagag ttgatactgc cactccgacc aaaccatagc gccactcaaa aatagcaaaa
    15481 cctgataaga ctgcattgag gaaattgaca aaaatactga catacattgg taaacgaggt
    15541 tgcccttgca cacgtaaaaa tgagcctagg gtggttaaca ttcctaaagt aacaacacct
    15601 ccacccacaa ttgctaaata taaaccacca acttgtgcga cactttttgt tgtccctaaa
    15661 agtttaaaga aagtttgtcc aaaaacaatc gagataattc ctaatactgc ccctatcagt
    15721 aatgttagaa aaatggcttg agaacatact gaaattgcat catccttctt actacctgct
    15781 aacgacttgg ccaatagact tgctatactc gcccctaaag ctataaaaat agcttgataa
    15841 atagtaatta tattattagc aactgaaaca cctgatactg ctacaacacc taactgagcc
    15901 actagataat tatcaactac tcccatgagc atttgtaaga tattttcaat catagctggt
    15961 atagctaact gaataatctc tttactataa cgcacagtat ctcctttcta ttacccagga
    16021 tcattaaaaa gagagtgatg ggaaaatttc taaaatgttt ctttctcgct ctaaatattt
    16081 taaaacaatc gtatttttat tattgtttca aacctagaca ctcttcaacc gtttctcgca
    16141 tatttgaggt aggtactacc gttctatgga ttacttcggc cttttgtaga tctgtaaccg
    16201 ccttaggtaa agaaacacct gacaaatcgt ttaagattga aatagcagcg aaatcctgta
    16261 tctcttcttt atttgtaata gcctttgtaa caacacacgg gaacttataa ggacttgctg
    16321 ttgacacaat tactgctgga gtttgatctt tagtttctgt ctgataagct tgataaacag
    16381 ctgatgctac agctgtatgc ggatcctcta tatactgata ctggtcaaat aaatgtttga
    16441 tgtctaattc aacaaattgt tctgtcgcaa atcccgctac aaagctctct aaaatatttg
    16501 cttgcctagc ctctaaggca tactccccag tagtcactaa atcttccatt aattttttag
    16561 tcgtttctgc atcatcgcct aaaaggtgaa agattaatcg ttctagatta gaggagacca
    16621 aaatatccat agagggactt gatgtcactt taaattctcg atttttatcg taggtttgtg
    16681 tcttaaaaaa atctgttaga acgttattat catttgaggc acaaatgagt ttggtaacag
    16741 gtaatccaat ttgactagca taatatgctg ctaaaatatt accaaaattt cctgtaggta
    16801 cactaaaatt aattggttga ccaattgaga tttcctttga cttaactaat tgagcatatg
    16861 catatatata gtaaacaatt tgaggaacaa ggcgaccgat gttcattgaa ttggctgatg
    16921 ataactgcat atgctgctgt gataatttta atcgaagaag ggaattattg aacatttctt
    16981 taactgaagt ctgtgcatca tcaaaatttc cttcaatcgc aacaacatga gtattttgtc
    17041 cggcttgtgt tatcatctgc aattcttgga tataactaac accattttta ggataaaaga
    17101 caataatctc agttcctgga acatctgcaa aacctgccat cgctgccttg ccagtgtcac
    17161 ctgatgttgc tgttaaaata acgattttat tatcaacccc ttgttttttg gctgctgttg
    17221 ttagcaaata aggaagaatg gataaagcca tatctttaaa tgcaattgta ggaccgtgaa
    17281 ataattcaag gtgataccgg tctccaatct ttactatcgg ggctatttca cttgtatcaa
    17341 atttagtatc ataagcttga ctaatacagt aatccaattc ttgttcagta aaatcatcaa
    17401 aaaaagctga caaaacgagt ttagccactt cttggtagga agcatcctta agtttagtga
    17461 aatctaaatc aacttttgga aatgtgatgg gggtaaagag tcctccatcg tcagctaatc
    17521 cctttagaat cgcttgacta gctgtcactt tattactagc atcacgtgtt gattgatata
    17581 ctattgccat aagaatccct ctttatataa atatctttta gttattgtaa cacaatttaa
    17641 taacatgtcc attgttcgtt atataattaa ccccctttat taaaataaag ggggttcgta
    17701 acatcattat cactttgtaa agtcaagaac tttacgtcct tgaatcaatc ctctttccat
    17761 ttcatcgaat acttgaggag ctgtatccac aggcactttt tcaaccaccg gtactaccaa
    17821 gccttctgct ccaaatgcga aggcttcttc taagtcttta cgagttccaa ctaaggaacc
    17881 aaccacacga atcccatcaa gtactgtttt tacaatactc aattccatat attctgatgg
    17941 aagaccgaca gctaccactg tacctccagc acgaacgctg tcaatcgcct gattaaacgc
    18001 aactttagaa acagcagtca caaccactcc atgacaaccg cctgtctttt cttgaatata
    18061 tgccgcaaca tcttttattt ctttgccatt aactgtcaaa tctgctccaa cctctttagc
    18121 taattgaagt ttatctgcgt taatatcaac agctacaaca tgagcattga atactttttt
    18181 tgcatattgg actgctaagt ttccaagacc acctgcacca tacactgcaa tccactgacc
    18241 aggagcagct ccagcttctt tgatagcctt gtatgttgtt actccagcac aagtgattga
    18301 tgatgcttga gctgggtcta atccctcagg aaccttaacc gcatagtccg cggtaacaat
    18361 agcgtattca ctcataccac catcaacact gtatccagca tttttaacac tacgacaaag
    18421 tgtctcacgt cctgtagtac agtattcgca atgaccgcac ccttcaaaga accatgcaat
    18481 agagacacga tcaccaaccc tcaaagacgt tacgccttct ccaatttctt ctactatacc
    18541 aatgccttcg tgacccaaaa tgcgtcctgg tacctggccg aaatctccat gtgcaacgtg
    18601 taaatcagta tggcatacac cacagtattc tacttttaca agtgcttcac catgaccaac
    18661 ttttggaaga tcatgctcaa caacttcaac acctgtacta gcttgattaa caacaaccgc
    18721 tttcatgata aaacctccta gaaataaata tatcttattt tgtaatcgtt tacattttat
    18781 attgtattat tcttcacaaa ctatgtcaag tgattttcag aaaatttttt gaaaatattc
    18841 ttaaccggtt aatctttttg aaactaaaaa tcccactcaa tagagtgaga ttcttcgact
    18901 gctgttactt acgacgtcct ggacgttcct cataaccata ataagcatct tcgatgattt
    18961 cttgcatgtg atctaccatc ggaaggcgtg gatttgctgg tgaacattga tcttcatatg
    19021 ctaagaaagc tagttcacga gatttttctt tccattcttt ttcatcaatg ccttggtcgc
    19081 ggaaattcat cttaatacct aaacgtgttc caaggtcata cacagctttt gcatatgatt
    19141 cgacagcttc ttctggtgtt gctgctggta agccgagtaa tttagcgata tcttgatact
    19201 tttcgtcagc acgatagtaa ttgtacttag gccatgttgc tgttttagct ggacgagttc
    19261 cattatagcg gataacatac ggcaaaagaa ttgcatttgt acgtccgtgt acggtatgga
    19321 attgtgcgcc aattttatgc gccattgagt gagaaatacc taagaatgca ttggcgaaag
    19381 ccatacctgc catagttgac gcattgtgca tcttttcacg tgcttcaaaa tcagcatctt
    19441 ttactgagcg ttctaagtag tcaaacacaa ttttaattgc ttgaagagca agtccgtctg
    19501 tataatcatt tgccatctgt gaaacataag cctctgttgc atgtgtcaat acatccatac
    19561 ccgtatcagc cgcaataaaa ccaggaacag tcataacaag ggccggatca acgatagcta
    19621 cagttggtgt taatgaataa tcagcgattg gatatttacg attatttgct ttgtcagaga
    19681 taacggcaaa tggtgtcact tcagaacctg ttccagaagt tgttggaata gcaacaaatt
    19741 ttgttttctt accaagttct gggaatttga acgcacgttt acggatatcc atgaattttt
    19801 gaacgaggtc atggaaatca acttctggtt gttcatagaa gagccacata actttcgcag
    19861 catccatagg agaaccacca cctaatgcaa taatagtgtc tggtttgaag gtacgcatca
    19921 aatctgttcc tttcattact gttgtaatgt ctggatctgg ttctacttca gcaaaaatct
    19981 gataaacaac tttattgcga cgaagatcaa gttgttcaat gatacgatcc aagaaaccaa
    20041 gctcaaccat ggcgtgatct gtaacaatca tgacacgttc aacatcgcga catttttgca
    20101 aatattgaat agaatcacgt tcgaagtatg tttttgaagg gactttaaac cattgcatat
    20161 tatttctacg tcttcctact tttttgatat tcaagagatt tatagcacta acgttatcac
    20221 caactgagtt acgtccatac gaaccacaac caagagtcaa tgatggcaag aaagcattgt
    20281 aaacatcccc aataccacca aaagtagaag gagaattcca aataacacgg atagcacgaa
    20341 ttctagttcc aaattcacga gctaagtctg catcttttgt atgaatagca gctgagtgcc
    20401 ctagaccatt aaactcaacc atttgacgag ctttttcaac accatcttct gtagattcag
    20461 ctttcaatac tgcaataaca ggtgatagtt tttcacgtgt caaaggttct ttttcactaa
    20521 cttcagtaca ttctgcggca agaatatttg ttccttctgg aacagtaaat ccagcttgtt
    20581 cagcaatcca aactgctgat tttccaacaa tatttggatt taattttgca ccagcacagt
    20641 ttttgctatt tgcttttgca ccaaaacaga attcttcaag aagagctttt tctttcttat
    20701 taacgaaata ggtatgataa gatttaaatt cttcaacaaa ttctttataa atttctttat
    20761 caataataac agcctgttct gatgcacata ccataccgtt atcaaatgat ttagacataa
    20821 caatatcgtg agcggcttgt cggatattag ctgatttttc aacataagct ggaacattcc
    20881 cagcacctac accaagggca ggtttcccac atgagtaggc agctttaacc atcgcattac
    20941 cacctgtcgc aaggatagtg gcaataccat catggttcat caaagcatta gttgcatcaa
    21001 ttgatggttg ttcaatccat tggacacaat tttcaggagc tccagcagca atagcagcat
    21061 cacgaacaat acgtgctgca tgtgctgatg attcttgtgc tgatgggtgg aaggcaaaaa
    21121 taattggatt acgtgtttta agagagatta atgacttgaa aatagcagtt gaagttgggt
    21181 tggttgttgg cgtgatacca cagacaactc ccactggttc agcaattaat gtcaagcctg
    21241 tgacatcgtc ttcttcaata actcccactg tctttgtatg acgcatgttg ttaacgacgt
    21301 gttcacatgc aaataaattt tttgtcgctt tatcttcaaa gacaccacga ccagtttctt
    21361 ctactgcatg aagagcaagt tctccatggg catccaaggc tgcaacagaa gctttagcta
    21421 caatataatc aacttgttct tgattaagtt gacgcatctc atcaagtgct tttaaaccat
    21481 tttgaaccaa tgtatcaatt gcaagagcaa catctgtagt ttctactgct ttagtttttt
    21541 cagtcatttg agactccttt tgatttgtta aatgtttcac aaatttgttt acgagattat
    21601 tatagcatat ttaaaaactt tgtaaatagt ttcacaactt ttttgtgaac tttttttcaa
    21661 tctttcggaa atatttcttg aaagcgcttt aattatattt gttttttcat tactaatttt
    21721 cagattaaag cctatcgatt tcatttttat caccataaac ggagatatct attattcgtt
    21781 ttcacaaaaa aagccgagta catgactcgg acttgtttat tttgcagctc gttgagctga
    21841 tttttgaata gcttttttga cgttactaac atataaatca gcccctgtag tatcgctatc
    21901 actataatgc acaccatctg tatttcccca gatttctgga tgttctgtcg caaccttata
    21961 ccagtcagca accgttatgt aattatattt tttcattagc gagagttcat aatcacgcac
    22021 tgtcgtaact tgactcatat ttttagcatt ataaggtgtt actataatca aacggtgtcc
    22081 cttaggcaaa gatttaataa attggctcaa gtctgtcttg tagttatcta atgaattcac
    22141 accaactgcc aaaacaacaa ttttggacaa ggctttattt tgaatacggt tttcaaaaat
    22201 atcaaatgat ttactaaaat tacgactcac cgcagcatct aattgtactt ctggtaacaa
    22261 tttattaaaa gcagcacttg accttaaggc aactgaatct ccgattactg tcacatcact
    22321 aagagcacct gcatcccctg cggctagtgt atgtgttcgg ttagtatttg ttctagcttg
    22381 ttgtaacgaa ttctgcaata attctgtctc aaaactacct atacttggag ctgtcaacat
    22441 tgtcactaca gtaataagag ttagtacccc tccgatacta aataaccact ttttgtaagg
    22501 caacaagtca aattcataat caaggaattt aggttttcta cctaaaataa aaggttcaat
    22561 gatataaaat gataatgttg agaagactgc tgataatact actgtcaaaa taacagctat
    22621 ccagtttggg gataaacgac taaaaataat ataaaatggc caatgaaaaa ggtacatacc
    22681 gtaactgata tcagcaagat aagtaatcac aaaaggttct gaaatatctg gtgtatgctc
    22741 gtgtaaaata cgtgcattgt aaatcattac actagcaaaa atgctagaca atacaaaacc
    22801 aaaaagatat gttagccgat tatcaaaatc aagtgcaaaa gttaaaatca ttagaccagc
    22861 aaaagcacta cccatcatga ttaaattatg cttcaaagtc agatttttga tatttttctt
    22921 gaatcgtccg gtaatctctc gaataccaga tattgtagcc accatcgctc ctaaaaagaa
    22981 cggaaatata tgtgataatg tagagaaata tatcgttgaa aaattatcaa cgaaaaaggc
    23041 tctaacaaac atggtaagga agctgactcc aaaaattccc atggaaataa ggaacaaagt
    23101 ccccctcaat tgcttttgat cttttgatct tttcgataag agccataccg ttaagcccca
    23161 taaaacataa aaatggactt caatagaaag actccaggta tgcacaaata agtgagggat
    23221 aaattgactt tcgtagttac caccagtcaa gatttcgtaa aagttgctgg taaaacctaa
    23281 agcagtcata atttgagagc caatacttgc tctaaaatcg cttttaacca gaaatacaaa
    23341 aggtattgtc accaaaacca tcaatactaa tggtgggaaa atacggtaaa aacgtctacg
    23401 acaaaaacta acaaaatcaa tttttttcgt tttagaaaat tcatcaatca ataaggccgt
    23461 aatcagaaaa ccagaaaatg taaagaagat gtcaacgcca acaaatcctc ctggaaaact
    23521 attcttaaag aaatggtaaa gtaaaacaag tagtagtcct gttatacgta ccaaagaaaa
    23581 ccattttatt ctcatttttg ataaatcccc tgtttaaatg tccccttata gactttctta
    23641 tttttagcat tgaggcgacc ttgtccatca ggttgacctt ttttaaagtc acctgtataa
    23701 gaccagccat gaactgaaac aaatgttcct ttcccttcaa agactccatt tacaaaatcg
    23761 cccttatagt aatccccatt ctcataggtt aatttacctt taccagtcat atggtgatta
    23821 acaatagagc ccacatattt tatcttgccg ccatcatagc taaagactcc ttgactagaa
    23881 gtcgttaagg taaatactga taaaccaaaa acaacaataa ttatcaaaga taatatttct
    23941 agatgagttc gtgttatttt aaaattcttc atttctactt aatctctact attatttatt
    24001 agcgttaacc actgtttaca gccatcattg actagacgat aggtttcctc aaaatcacca
    24061 gtataccatg gatcaggaac accaccatca caaaaaagac taatcttatc atcataggtt
    24121 ccttgtgata gagcttttaa atccttgaca ttttgctcat ccataccgat gatataatca
    24181 aaatatgcta aatcgtcttt agaaataggt tgtgaccctt tatcgttaat gtaaggaatt
    24241 tgataacgct gcaaaatcgc ttgcgttccc ctatgtatag gatttccatg ctcccatgtt
    24301 gaagtcgcac gactctcaac caagaggcta tcctccttat ctaaattcgc cataacaaac
    24361 tcagccatcg ggctacggca gatatttcca agacaaacaa aacagacttt cttcatagca
    24421 aactcctttt ggtctattat aacagaaaaa aggtacttat aatacgttta actgctcaaa
    24481 gaataactac aacatatcta atacttaaca ttgcatgaaa attaaaaaat taagactaga
    24541 tactctagcc ttaaaatgac ttatgtttta tttatcaaat cgctgataac ctaaatgttc
    24601 atatgcctta tccgtagcta cacgaccggt acgggtacgc ataataaaac ctttttgaat
    24661 taaataaggt tcgtacatgt cttcaacagt atctcgttct tcagcaatat taacggatag
    24721 agttcctaaa ccaacaggac ctccattata catttcaatc atggttctta agattttttg
    24781 atcgacgtaa tcaagcccct cgtgatcaac atctaacatc gttaatgctt tatctgtaat
    24841 attgtcatct atcaaaccat ctcccatgat ttgagcataa tctcgaacac gtttcaataa
    24901 acggttagcg atacgtggcg ttccacgact gcgacgcgct aattcagaag cagcttcata
    24961 agtaattttc atttcaaaaa tgtctgctgt acgctcaata atttctgtca aatcattttc
    25021 ttcataatac tccatatgcc ctgtaatacc aaagcgagca cgtaagggat tagataacat
    25081 acctgcacgt gtcgttgcac caattaaggt aaagggcggc aaatctagat gaacacttct
    25141 actagtttct cctgcaccga tcataatgtc aatatagaag tcttccattg cactataaag
    25201 tacctcttca accgccatcg gcatacgatg aatttcatca ataaaaagaa catcacctgg
    25261 ttctaaatca tttaaaatgg ctactaaatc ccctgatttt tcaattgcgg gacctgatgt
    25321 ttgtttgaga ttgacaccca actcattagc aattacaaat gccatggttg ttttccctaa
    25381 accaggaggg ccaaataata acacatgatc caatgactca tcacgcaatt tagcagcttc
    25441 aataaatatt tttagctgat ctttaacctt atcttgtcca atatactctc ttaaatactg
    25501 cggacgaagt gtacgttcta ccaattcttc gtcacccatt gcatcactat ctaaaaatct
    25561 tgtcatgata ttattataac atttgtatag gaaaagttaa gaactaagtt ggtgtattag
    25621 tatctccctt atttaaagga agaataaact gatcttatag ttaatccaac ctcaagaaat
    25681 cacagaacag cttacttagc ccatattcca ccaacccatt cctaaaaaca aatcaaatga
    25741 acctaaattt gataatagaa caagtaatac tattaaaaaa cattttttaa tcactataat
    25801 aaactcctaa ataatatttt gtacgtttat tataaatttt tagacctcag agtcacagga
    25861 catcaatgtc ctgttatatc ttcttgccac tccaattcta attttttaat tagatgcttg
    25921 tcatcaatca tttttgaaaa cataattgat tgttggtaag cattttctgc agactttata
    25981 tcattgtcaa ttgtaatgaa gtatttccat tggtacataa aatataaagg cattttatga
    26041 aaatcatgtg ttgacaccat cgtttgtcgg ctaacttcta acatttctgc aagacactct
    26101 tcactattta aacaaagtcc aacaaataca caattcagga gtacatgatt aaaccaaaac
    26161 aaatcttctg ctgtaagttt gtggttctga cttatcaaag ttttacatat agtttgaaat
    26221 acttctttag taaatgctct attgttaaat ttagatacaa ctgcacaagt taaaaacaaa
    26281 tctatgataa tcaaatcatt aagagtatat ttttctttat attttacttt atcaaagcat
    26341 tcgcataaaa cctccacacc aaaattaatg tctcctgttt gatagacttc aaatttagct
    26401 tgtaaactgt caattgctaa gcactcgtct tctggaagat tatcatagaa ttcttcgaaa
    26461 atatgatcaa actgacattc tcttaatttt aatcgttcag catctgcata agttggaatt
    26521 cttaaaatca aatacttcaa ctctttatac cgtttaggta attctaagct ctctcctcct
    26581 gttaaaatat ccaattttac attcaattgt ttagctatgt agtgagcttt agtcaagttt
    26641 ggaattgact gtccactttc aatcctagct aattgtctta cagataactc agtttcatca
    26701 ccacaaaaat cttcgcgagt aatatttcta gttactctca aatttcttac tttactacca
    26761 aaatgtttta acattttcct ttaaaaacta actcctaaat ttttcttctt attatatatt
    26821 atcataataa actcatttta agtatgtttt ttaatattaa ttccttttaa atcaataaaa
    26881 attccattac tggttaaaaa aacgttaacc agtaatggaa ttttcttatt tttctaatcc
    26941 tagtctttca aaaatatcat caacacgttt tgtgtaataa atcggattaa atagttcatc
    27001 aatttcttcc tgtgtaagac aagaggtaac tttggtgtct tcttctaaaa gtggtttgaa
    27061 atccacttga ttgtcccagg aataagctgt cttaggttga actaagtcat aagcttcttc
    27121 tcgtgtcatt cctttttcaa tcaatttgag cataacacgt tgactataaa tcaaaccaaa
    27181 agttgattcc atattgcgca tcatattttc cgggaagaca gtcaagttct taacgatatt
    27241 gccaaaacgg ttgagcatgt agtcaatcaa gatcgttgtg tcaggtgtga tgatacgctc
    27301 agcagatgag tgcgaaatat cacgctcgtg ccaaagtgcc acgttctcat aagccgtcac
    27361 catgtgacca cgaatcacgc gcgctagccc agtcatgttc tctgaaccga ttgggttgcg
    27421 tttgtgaggc atagcagagc tacctttctg acctttggca aagaattctt caacttcacg
    27481 ttgttctgat ttttgcagac cacgaatctc tgtcgccata cgttcgatag aagttgcaat
    27541 gcttgcaagc actgcaaaat attctgcgtg gaggtcacgt ggaagaacct gtgttgaaat
    27601 ctcttgcgga cggatgccca atttgtcaca aacatattgt tccacaaatg gaggaatgtt
    27661 agcaaagtta ccaacggcac ctgaaatctt accagcttcc acacctgcgg cagcatgttc
    27721 aaaacgctca atattacgtt tcatctcgct gtaccaggtc gccaacttaa gaccgaaagt
    27781 cgttggctca gcgtgaacac cgtgggtacg acccatcatg atggtgaact tgtgctcctt
    27841 agccttatca gccacaatat ttgtgaaatt ctcaaggtca cgacggataa tatcgttagc
    27901 ctgcttgtag aggtaaccgt aggcagtgtc caccacgtca gtcgacgtca aaccgtagtg
    27961 cacccacttg cgctcctcac caagcgtctc agaaaccgca cgagtgaaag ccaccacatc
    28021 gtgacgcgtg tcctgctcaa tctcaagaat gcggtcaatg tcaaaatccg ccttctcacg
    28081 aatcttagcc acatcctcct taggaatctc acccaactca gcccatgcct cgtcagccaa
    28141 aatctcgacc tccaaccaag cacggtattt attttcctct gtccaaattg ccgccatctc
    28201 agggcgtgaa taacgttcga tcattttgtt ttactccttt aaaaaattct aattactacc
    28261 cttagcttta tcatattctt tttcaaaata attcaaagat tcttcagtta aataatccga
    28321 cacttctttt aatatttctt tagctttttc aacttctcct aaagaaatta agatgtatat
    28381 gtattcctca taaagtttag gtataaatat cttttgatta ttatgatcaa atattgattc
//
LOCUS       DATRNK010000021        26884 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.021, whole genome shotgun sequence.
ACCESSION   DATRNK010000021 DATRNK010000000
VERSION     DATRNK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26884
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.021"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            275..1297
                     /locus_tag="VBC52_000931"
     CDS             275..1297
                     /locus_tag="VBC52_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6882528.1"
                     /translation="MNIIIGTSLLILVLAIFTLFNYKAPYGTKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVTIAIGVTPGYAVLIGLSVSGTG
                     IIPGFLAGYLVGFLVKWMERNIPGGLDLISIIIIGAPLTRLVAKLLTPLINSTLLTIG
                     DILTSGAHSNPILMGIILGGTIVVVATAPLSSMALTAMLGLTGMPMAIGALSVFGSSF
                     MNGVLFHKLKLGSRKDNIAFAVEPLTQADVTSANPIPIYVTNFVGGAACGILIALMKL
                     VNDTPGTATPIAGFAVMFAYNPMIKVLITALGCIILSLLAGYFGGIVFKDYKLVTKEE
                     LQARDN"
     gene            1668..2960
                     /locus_tag="VBC52_000932"
     CDS             1668..2960
                     /locus_tag="VBC52_000932"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEO6882529.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDNKKFKLHLIPSGIFFKEKISVIGNGVVVNPKSLVKELAYLHGEGVTTDNLRISDRA
                     HVILPYHIKLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDREVFAERL
                     KINLAEKNRLFEKMYDSTPLEFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGDRIREIGKEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPASLEQLKRCKPIYEELPGWSEDITACRSLDGLPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWSNI"
     gene            complement(2983..3276)
                     /locus_tag="VBC52_000933"
     CDS             complement(2983..3276)
                     /locus_tag="VBC52_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882530.1"
                     /translation="MILINLIALPICFLAELLAGLLLKLEMHKQHGYYPLERLLMTGS
                     ILTVVSLVLLHTLIHSINALLFLSFTVVLLISGKFLSLISIYLLKRKSKHQKV"
     gene            3615..4232
                     /locus_tag="VBC52_000934"
     CDS             3615..4232
                     /locus_tag="VBC52_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882531.1"
                     /translation="MRKFFQDVIVEVAYYLEIALSVILATAIIFFSLALFHDLFSMLS
                     VDQNVDKLFQTFLARAMTIAVGIELIKMFSKPSPDTLIEVLLFALARQLVIEHGSVSD
                     YLMGIASVAILFAVRKYLFTHFDGSSNIVLRANNKVKKANILARIQLPCQPNETLGDL
                     LVRHLNDEEKDVAIGSVVYINNVALRVDSMHDGLVTRVEIIRSLH"
     gene            4400..5839
                     /locus_tag="VBC52_000935"
     CDS             4400..5839
                     /locus_tag="VBC52_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6882532.1"
                     /translation="MENFLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPLHDVFAG
                     FIKATVGYLILNVGAGGLVNTFRPILVALAKKFNLEAAVIDPYFGLASANAKLETMGF
                     ISVATTALLIGFGINILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQLRNG
                     FGAWAVGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGGILAVLGPDIMSNVKLIGPGAFVPTKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVTELTNAFQGISNKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVVFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGIIQVALGAVAVGLLGLAGGYHGNIDFEFPWLAFGYIFKYLGIAGYVIV
                     CLFFLAIPQLQFMKSKDKEAYYRGDAPAE"
     gene            5867..6145
                     /locus_tag="VBC52_000936"
     CDS             5867..6145
                     /locus_tag="VBC52_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6882533.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVSNFESASCSVGEA
                     KGLAANYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEIL"
     gene            6212..6697
                     /locus_tag="VBC52_000937"
     CDS             6212..6697
                     /locus_tag="VBC52_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6882534.1"
                     /translation="MNLKQAFIENDSIRLKLSASDWKEAIKLSIDPLIESGAVDAEYY
                     DAIIESTEEFGPYYILMPGMAMPHARPEAGVKRDAFSLITLTESVVFPDGKEVSVLLA
                     LAATSSAIHTSVAIPQIIALFELENSIQRLTECQEAKEVLAMVEESKNSPYLEGLDLE
                     S"
     gene            6810..7475
                     /locus_tag="VBC52_000938"
     CDS             6810..7475
                     /locus_tag="VBC52_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEO6882535.1"
                     /translation="MTKRLPNLQVALDHSDLQGAIKAAVSVGHEVDVIEAGTVCLLQV
                     GSELVEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKEERGDRGEIQIELYGDWTYEQAQQWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLSTDTLQLFEGVDVFTFIAGRGITEADDPAAAARAFKDEIKR
                     IWG"
     gene            7479..8342
                     /locus_tag="VBC52_000939"
     CDS             7479..8342
                     /locus_tag="VBC52_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEO6882536.1"
                     /translation="MTRPIGIYEKATPKHFNWLERLQFAKELGFDFVELSIDESDERL
                     ARLEWSKEERLELVKAIFETGVRVPTITFSGHRRFPMGSNDPEKEARAMDMMKKCIVF
                     AQDIGIRNIQLAGYDVYYEEKSPETRARFIKNLRQACTWAEEAQVILSIEIMDDPFMN
                     SIEKYLAVEKEIDSPYLFVYPDTGNVSAWHNDLWSEFYNGHRSIAALHIKDTYAVTET
                     SKGQFRDVPFGQGCVDWEEMFAVIKKTNYNGPFLIEMWSENCETVEETRAAIKEAQDF
                     LYPLMEKAGVK"
     gene            8344..9060
                     /locus_tag="VBC52_000940"
     CDS             8344..9060
                     /locus_tag="VBC52_000940"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEO6882537.1"
                     /translation="MAKSLQEMRERVCEANKSLPVHGLVKFTWGNVSEVDREAGLIVI
                     KPSGVDYDQLTPENMVVTDLEGNIVEGDLNPSSDLPTHVQLYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLSEDEVNTAYEKETGSVIIEEFERRNL
                     DPMAVPGIIVRNHGPFTWGKDPAQAVYHSVVLEEVAKMNRFTEQINPRVEPAPKYIMD
                     KHYLRKHGPNAYYGQKGQEH"
     gene            9071..9718
                     /gene="fsa"
                     /locus_tag="VBC52_000941"
     CDS             9071..9718
                     /gene="fsa"
                     /locus_tag="VBC52_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO6882538.1"
                     /translation="MKYFLDTADVSEIRRLNRLGIVDGVTTNPTIISREGRDFKEVIN
                     EICQIVDGPVSAEVTGLTCDEMVTEAREIAKWSPNVVVKIPMTEEGLAAVSQLSKEGI
                     KTNVTLIFTVAQGLSAMKAGATFISPFVGRLEDIGTDAYALIRDLRHIIDFYGFQSEI
                     IAASIRGLAHVEGVAKCGAHIATIPDKTFASLFTHPLTDKGIETFLKDWDSFKKK"
     gene            9882..10865
                     /locus_tag="VBC52_000942"
     CDS             9882..10865
                     /locus_tag="VBC52_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6882539.1"
                     /translation="MFEKVTISDIANLVGVSKATVSYYLNGNYKKMSLQTKEKIRLAI
                     KETGYQPSKIAQSLVTKNTRTIGVVIADITNPFISSVMKGIHDTCQQFGYSVNFTNSD
                     NDIDIELENLNRLNQQNVSGIILDSVDPNHSFIETLSNDRLVMVDRQAKDIKVDTVVS
                     DNKESTQIFLEKMQETGYHDIYFVTYPIEGISTRELRYEGFKEVVSSNPDKLIIITED
                     GSTQRILDIIEHSEQKPGFLMMNGPTLLNFMKKLNQSTVSYPEDYGLGSYEDLEWMQV
                     LTPNVSCIKQDSYGIGCLAAQCLIEKISQGNEPTTARLLEVKNQIVIRQSF"
     gene            11100..12119
                     /locus_tag="VBC52_000943"
     CDS             11100..12119
                     /locus_tag="VBC52_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882540.1"
                     /translation="MKINKLLSDRLEACYSVCRLQNQDYDFLLVASEVDQACFAYDLN
                     SDLAKIVVWEDVGGTMSIIPIPGTMDFLATQKFYPGFKAKDCQVVYASFQGDHWDICN
                     AFDFPYLHRFDLIKGKENQLYFIGCTIANSKAYTEDWSDKGRIFVARFNTQNHTLEGL
                     QQLPQTLLKNHGYYAIQDEGYSLITSVEGVFKLTYPEFSTTGDWQLERLFDEETSDVV
                     KVDINQDGKDEYMIIQGFHGDRLRIFTEDFGRELFHYPEKTPFGHAIWSGRLLNQTCF
                     VFGWRSEKAELRLFHFVDGHLVSELVDAKAASSNVLAFEKDGKAYLFSANNGRGEVAL
                     YQLVK"
     gene            12138..13094
                     /locus_tag="VBC52_000944"
     CDS             12138..13094
                     /locus_tag="VBC52_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO6882541.1"
                     /translation="MDKKKILVTGIVPKEGLRKLMDRFDVTYSEDRPFSRDYVLEHLS
                     EYDGWLLMGQKGDKEMIDAGENLQIISLNAVGFDHVDTAYAKEKGIVVSNSPQAVRVP
                     TAEMTFALILAASKRLAFYDSIVRSGEWIDPSEQRYQGLTLQGSTLGIYGMGRIGLTV
                     ANFAKAFGMTVVYNDVYRLPEDKEKELGVTYLEFDQLIETADVITIHAPALPSTIHKF
                     NKDVFAKMKSRSYLINAARGPIVSEEALIEALKEGEIAGAGLDVFENEPQVSEGLRSL
                     DNVIMSPHAGTGTIEGRRTLAEEAADNIIAFFDGKPQNIVNK"
     gene            13192..14643
                     /locus_tag="VBC52_000945"
     CDS             13192..14643
                     /locus_tag="VBC52_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO6882542.1"
                     /translation="MLEALHKFFEVFGAATVVPAMIFIVSLFLKVNPRRAFFSALRAG
                     VGLTGFGWIISAFAPMVTKYIQQMVKTTGLHLPIVDIGWQAGSLTAFSSEIGLSFFVF
                     GLLIELGLFLLGITRVFVPSNLWNNFGYMIWGTMAYAATGNFILSFAFMVFVLLYSLV
                     MSEVLADRWSEYYGVKNATINSIHNIETLIPALILDPLWNLLSVNKVKLNPESLKTKL
                     GIFGEPMTLGFILGVIIGVLGSLRNLASIDTWGGILGFAVALAAVMTIFPLITGVFAS
                     AFAPLAEAVERNKKKESQAEQGALDKKRWFIAVDDGVGFGEPATIIAGLILVPIMVVI
                     SLILPGNEALPVVDLIAIPFMIEAMIAVSKGNILKAILNGIIWFSLGLYAASALGPIY
                     TEAVKHFGTALPAGVTLIMSFNLLAHPLTALVFFAWISGNPLYIGVTVVIYLAFLFLL
                     RTRRESIYAYLKTMADKNSGYEGSCEKIFIAED"
     gene            14665..15654
                     /locus_tag="VBC52_000946"
     CDS             14665..15654
                     /locus_tag="VBC52_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO6882543.1"
                     /translation="MKKIDGHAHIVDTIAGFNGKGRLNALGNGYAIWDDGDLIKLIPD
                     EYGDKAFTAEAFLKYMDANGVDKAVILQGHLNGYQNYYTHLAIKRYPERFTGAFSVDP
                     FADNAMQIVKRHVEVLGFRAIKFEISQGGGIHGYRGQKTPFRLDTDPHVSRILTYLLD
                     YPGFVVTVDYGNWDQISHQPDAIANLARLYTSLDFVVCHLSFPHVEHSNRLRAELNMW
                     KNFNNIYTDISAIQDIDAPDSFPFPKSEQNVRIAKEVLGAKRIIWGTDSPWSATFNTY
                     EELATWLENVDIFSQEELEDVMYNNAERVYFKEEHVKANRAGIDDTTKELNLY"
     gene            15720..17237
                     /locus_tag="VBC52_000947"
     CDS             15720..17237
                     /locus_tag="VBC52_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEO6882544.1"
                     /translation="MTYYLSIDYGGTNTKALIFDKLGHQIAVSSFETLKNEAQSGHRQ
                     VNLVKTWNAITSAIREVIQISKLSPEQISAVACIGHGKGLYLLDNKLEPLEQGILSTD
                     NRAKDLAQYFESKLDNIWELTRQHIFPSQSPVILRWLKDYQPETYKSIGAVLSAKDFI
                     RYKLTGKVQQEYGDASGNHWINFQTGTYDPAILDFFGIREIENSLPELIDSADLVPGG
                     ISSQAAKETGLVEGTPVVGGLFDIDACALGSGVLESDTFSVISGTWNINTYPSLKPAK
                     QDSGLMTSYFPDRRYLLEASSPTSAGNLDFMLKMLMHQEIDNAKSSGGSIYDNLEEFL
                     THTDATHHGLIFFPFLYGSNTSQDASACFFGLTTKSTKSQMIRAVYEGIAFAHKQHIT
                     DLIKSRGGVPKIIRFSGGATNSPAWMQMFSDILNLPIETVEGTELGGLGGAILAHHAL
                     DKISLKEAVQDMVRVKAIYKPQLSEVKGYEKKYHAYQKLLETLDPIWSELGHLNK"
     gene            17257..18018
                     /locus_tag="VBC52_000948"
     CDS             17257..18018
                     /locus_tag="VBC52_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP endonuclease"
                     /protein_id="HEO6882545.1"
                     /translation="MKQEDIIINSIVFKKQLDKGFTQVQLIKLVKDLGFSRFEIRQEL
                     LQDPDRELPALKAEADFYDINLYYSANEDLIKGGKVNPYLNKGLKEASQLGAPFIKLN
                     VGQTRNLSKEELEPLKEILKSQTIGIKVENNQDPKAATVENCQYFMTLVKELQIPISF
                     VFDTANWAFINQDLYQAVNSLACDTTYLHCKNFLQVAGKPHLSKSLFEGEIDLTDLLK
                     SFSNCEYLALEYPTELEVLKRDVQRLISISNSQNF"
     gene            18084..19763
                     /locus_tag="VBC52_000949"
     CDS             18084..19763
                     /locus_tag="VBC52_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEO6882546.1"
                     /translation="MIILDKKSYDLLFYLLKLEEPETVMAIANALNQSRRKVYYHLEK
                     INDALPSDVPQIVSYPRVGILLTEKQKAACRLLLDEVTDYSYVMKSSERLQLSLVSIV
                     VAKDRITIDRLMQLNDVSRNTILNDLNELRSELAEKEYNLQLQSTKCRGYFLDGHPLS
                     IIQYLYKLLDDIYHNGSSSFIDLFNHKLSQAFGASTYFSKEVLDYFHHYLFISQRSLG
                     KKINSQDGQFMIQILPFILMAYRKMRLSPEVQTSLNSDFSLVWQRKEYEIAKELADEL
                     EENFQLSLDEIEVGLVAMLMLSFRKDRDNHLESQDYDDMRATLTSFLKELEERYHLHF
                     VHKEDLLRQLLTHCKALLYRKRYGIFSVNPLTEHIKDKYEELFAITSSSVKLLEKAWQ
                     IKLTDDDVAYLTIHLGGELRNSQQSPNKLKLVIVSDEGIAIQKLLLKQCQRYLTNSDI
                     EAVFTTEQYQSVSDLMHVDMVVSTSDALESRFPMLVVHPVLTDDDIIRLIRFSKKGNC
                     ANSNQFTNELEKTIAQYVKEDSERYVLKSKIEKLIHQELLQDVLPLQSTVC"
     gene            20080..21171
                     /gene="ulaG"
                     /locus_tag="VBC52_000950"
     CDS             20080..21171
                     /gene="ulaG"
                     /locus_tag="VBC52_000950"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEO6882547.1"
                     /translation="MPNVKDITRESWILSTFPEWGTWLNEEIEEEVVAEGNFAMWWLG
                     NCGVWIKTPGGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVI
                     DPFAINELDYYLVSHFHSDHIDINTAAAIINNPNLDHVKFVGPYECGEIWKKWGVPEE
                     RIIVIKPGESFEFKDIKVTAVESFDRTCLVTLPVDGAEEHDGELAGLAVTDEEMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDYKIDVAINNYGDNPVGIQDKMTSIDLLR
                     MAENLRAKVIIPVHYDIWSNFMASTDEILQLWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DKDRIEYHHPRGFDDCFEQESNIQFKALL"
     gene            21261..23639
                     /locus_tag="VBC52_000951"
     CDS             21261..23639
                     /locus_tag="VBC52_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEO6882548.1"
                     /translation="MSEFDTKSYLEKLDAWWRAANYISAAQMYLKDNPLLRRELVEND
                     LKVHPIGHWGTVPGQNFIYAHLNRAINKYDLDMFYIEGPGHGGQVMVSNSYLDGSYTE
                     LNPNIEQTEDGFKQLCKIFSFPGGIASHAAPETPGSIHEGGELGYALSHATGAILDNP
                     DVIAATVIGDGEGETGPLMAGWLSNTFINPVNDGAVLPIFYLNGGKIHNPTIFERKTD
                     EELSQFFEGLGWKPIFADVVELSEDHAAAHALFAEKLDQAIQEIKTIQSEARQKPAEE
                     AIQAKFPVLVARIPKGWTGPKAWEGTPIEGGFRAHQVPISVDAHHMEHVDSLLSWLQS
                     YRPEELFDENGKIVDEIAAISPKGDRRMSMNPITNAGIVKAMDTADWKKFALDINVPG
                     QIMAQDMIEFGKYAADLVDANPDNFRIFGPDETKSNRLQEVFTRTSRQWLGRRKPDYD
                     EALSPAGRVIDSQLSEHQAEGFLEGYVLTGRHGFFASYESFLRVVDSMVTQHFKWLRK
                     SKTHTTWRKNYPALNLIAASTVFQQDHNGYTHQDPGILTHLAEKTPEYIREYLPADTN
                     SLLAVMDKAFKAEDKINLIVTSKHPRPQFYSIAEAEELVAEGYKVIDWASNVSLNQEP
                     DVVFAAAGTEPNLEALAAISILHKAFPELKIRFVNVLDILKLRHPSQDARGLSDEEFN
                     KVFTTDKPVIFAFHGYEDMIRDIFFSRHNHNLHTHGYRENGDITTPFDMRVMSELDRF
                     HLAQDAALASLGHEAQAFSDEMNQMVAYHKDYIREHGDDIPEVQNWKWENIK"
     gene            24015..25238
                     /locus_tag="VBC52_000952"
     CDS             24015..25238
                     /locus_tag="VBC52_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO6882549.1"
                     /translation="MTNILEVKNLTKIFGKKQKAALEMVKQGKSKTEILEKTGATVGV
                     YDASFEIKEGEIFVIMGLSGSGKSTLVRMLNRLIDPSSGNIYLDGKDIAKMNVEDLRN
                     IRRHDINMVFQNFGLFPHRTILENTEFGLEMRGVSKEERTTLAEKALDNAGLLPFKDQ
                     YPSQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDTNKQTI
                     IFISHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTVLEIDGPQVALTRMHREEVSMLMATNRRRQLLGSLTADAAIEARKKGL
                     PLSEVIDKDVVTVSKDTVITDIMPLIYDSSAPIAVTDDNDRLLGVIIRGRVIEALANV
                     QDETVVESPKETVEA"
     gene            25257..>26884
                     /locus_tag="VBC52_000953"
     CDS             25257..>26884
                     /locus_tag="VBC52_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEO6882550.1"
                     /translation="MENLLQHKLPVAPFVESATNWITKTFSGLFDFIQTIGNALMDWM
                     TKTLLFINPLLFIVLITAAVFFLAKKKWQLPTFTFIGLLFIYNQGLWEQLINTFNLVL
                     VASLISIIIGVPLGIWMAKSDKVKQVVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVVFALPPTVRFTNLAIREIPLELIEASDSFGSTAKQKLFKVELPLAKNTIMAGIN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGTGFVSGLSLVILAIVLDRVSQFF
                     NSKPGEKQAKTSKVKKWVGLGALALFILAALGRIVVNMTSGNEAKGQKVKIAYVQWDS
                     EVASTNVIAEVLKSKGYDVELTPLDNAVMWQTVANGNADFTTSAWLPKTHGQYFNKYK
                     NSLDDLGPHVENVKIGLVVPKYMNVNSIEELSNQADKQITGIEPGAGIMKSAKQSLKD
                     YPNLSSWKLLSASTGAMTTTLGKAIKNKDQVVITGWSPHWMFAKYDLKYLKDPKKSFG
                     GEEHINTIARKNLKKDMPKVYKIIDKFKWTKEDM"
BASE COUNT         8442 a         4413 c         5407 g         8622 t
ORIGIN      
        1 aataaaaaat tatcgtaata aaaaaaagtt aagaaaataa aaaattatta ttgactatgg
       61 ttatgctttc aggtaaaatg tgattatatt agcaactaag gagtaatcca tgagataagc
      121 ctaataatta tgtcggactt ctttattgca ccttatccag ttcgtagtac aattaagttt
      181 cagtattacc taaaggcgcc tttattgaaa ttgaagcagt agtacacaga cactgagaat
      241 aaaaaactta actattttta gcgggagtat atttatgaat atcattattg ggaccagtct
      301 tttaatactt gtattagcga ttttcacttt atttaattat aaagcaccct acggaacaaa
      361 agcaatggga gcattggcat ctgcggcttg tgcatccttc cttgtagaag cttttcaaga
      421 ttctttcttt ggtaaagttt tgggatttca atttttaagt gaagtgggtg gtgctaatgg
      481 ttcactttct ggtgtagctg cagctatatt ggttactatt gctattggtg taaccccagg
      541 atacgcagtt ttaattggtt tatctgtttc tggaacaggt attattcctg gtttccttgc
      601 aggatattta gtgggatttc ttgttaagtg gatggaaaga aatattcctg gtggtttaga
      661 tcttatttca attattatta ttggtgcacc attaacacgt cttgtggcca aactgttgac
      721 gccgcttatt aatagcactt tgctcaccat tggagatatc ttaacatcag gtgctcatag
      781 caaccctatt ttaatgggga ttatacttgg cgggacaatt gtagtagtgg cgacagcacc
      841 actttcttct atggcattga cagctatgct aggattaacc ggaatgccta tggctatagg
      901 agccttgtct gtctttggtt cgtcatttat gaatggtgta cttttccata aattaaaact
      961 tggaagtcgt aaagataata tagcttttgc tgttgagcct ctaactcaag ctgacgtgac
     1021 ttcagctaac cctattccaa tctatgtcac taattttgtt ggtggtgcag cttgtggtat
     1081 tttaattgcc ttgatgaaat tagttaatga tactcctgga acagcgacac caattgcagg
     1141 atttgctgtc atgtttgcct ataacccaat gataaaagta ctaataaccg ctctaggttg
     1201 tattatccta tctttactag caggctattt tggaggcatt gtttttaaag attataaatt
     1261 agttacaaaa gaagagctac aggctagaga taactagtta cttcatgaaa agctaatagt
     1321 aaatatcatt aatatagata tgatatctga cttgtgataa ctgttcttga taatttaaaa
     1381 ttcaacgact ctaataaaaa gatcgttgaa tttttttatt gttaagaatg ctggtatcag
     1441 taaatttcaa gatttcttta tcaactataa tgttagtttt ttaccgtttt tttacacttc
     1501 taataattat ttttttcttt ttttccgata atatttgagg aatttggcaa aaaaaaacat
     1561 tttttgctat aataaggtgt cagacaaact cgcaggtgaa attcctgcca tgtttttcca
     1621 gaaaggattg ggtagtccta aaaaggctca ctcagtgtta aataaatatg acttcagttg
     1681 ttgtagtagg tacccaatgg ggtgacgaag gtaaaggtaa aattacagat tttttatctg
     1741 ctgacgcaga agtaattgca cgttatcaag gtggtgataa cgctgggcat actatcgtta
     1801 ttgataacaa aaaatttaaa cttcatttaa ttccttcagg aattttcttc aaagaaaaaa
     1861 tttctgtcat tggtaatggt gttgtcgtta atccgaaatc attagttaaa gagttagctt
     1921 atcttcacgg agaaggtgta acaacggata accttcgtat ttcagatcgt gcgcatgtta
     1981 tcttgccata ccacatcaag cttgaccaac tacaagaaga tgctaaaggt gataataaaa
     2041 ttggaacaac catcaaagga attgggccag cttacatgga caaggctgcg cgtgtcggta
     2101 tccgtattgc tgaccttctt gatagagaag tttttgctga gcgtttgaaa attaatttag
     2161 ctgagaaaaa tcgtttattt gaaaaaatgt atgacagtac acctcttgaa tttgatgata
     2221 ttttcgaaga atattatgaa tatggtcaac aaattaagca atatgtgact gatacatccg
     2281 ttatcttaaa tgatgctctt gatgccggta aacgtgttct ttttgaagga gcacaaggag
     2341 ttatgctcga cattgatcaa ggaacatacc catttgtaac atcttccaat ccagtagcag
     2401 gtggtgtcac aattggttcg ggagttggac caagtaaaat taataaagtg gtaggtgtat
     2461 gtaaagctta cactagccgt gttggcgatg gaccattccc aacagaactt tttgatgagg
     2521 ttggtgaccg tattcgtgag attggtaaag agtatggtac aacgactggt cgtcctcgtc
     2581 gcgttggatg gtttgattct gttgttatgc gtcacagccg tcgagtatca ggtattacta
     2641 acctctctct gaattcaatt gatgttcttt cagggcttga tacggtaaaa atttgtgtgg
     2701 cttatgacct tgatgggaaa cgtattgact attacccagc aagccttgaa cagctaaaac
     2761 gttgtaaacc aatctatgaa gaattaccgg gctggtctga agatattaca gcttgccgta
     2821 gcttagatgg tcttccagaa aatgcacgta attacgttcg ccgtgttggc gaattggttg
     2881 gtgttcgtat ttctactttc tcagtagggc ctggtcgtga acaaacaaac attcttgagt
     2941 ctgtttggtc aaatatttaa tattaaaagt ctgactgtac agtcagactt tttgatgttt
     3001 actttttctt tttaatagat agatactaat aagacttaag aacttcccag aaatcaataa
     3061 aactactgta aatgataaaa aaagaagtgc attgatactg tgaatcaaag tatgtaataa
     3121 tactagcgat acaactgtta gaatactacc agtcatcaat aagcgttcca gaggatagta
     3181 gccatgttgc ttatgcattt ctaattttaa tagtagacca gctaacaatt ctgctaggaa
     3241 acaaataggt agagctatca aattaataag aatcatagac caaccaaatc cttgacccaa
     3301 acctatatat gatagactat ttttgggaat tgttgacgtg tgaattataa aatcaatcgt
     3361 aaggataatg tctataagaa gtagtagcgc taacagataa tgtagtaaga agattgttct
     3421 taaccgttta ttaatcaata tttcatttcc tcctacttat ttaccaataa gcttattata
     3481 tcataatgta taatcaaaca ttcgttagta ctgattaaaa ggtttatgaa ggggtggtca
     3541 ctattgtttc attgaattag taactttaac gacgaaatgt tataatgtta ttagtttata
     3601 aaagaggaga ttgtatgcgt aaatttttcc aagatgttat tgtagaggtg gcttattatt
     3661 tagagattgc tttgtcagtt attctggcaa cagctattat tttctttagt ttagcactct
     3721 ttcatgattt gtttagcatg ttgtctgttg atcaaaatgt tgataagttg ttccagactt
     3781 tccttgctcg cgccatgaca atagcagtcg ggattgagtt gatcaaaatg ttttcaaaac
     3841 cttccccaga tactttaatt gaagtgttat tgtttgcctt agctcgtcaa cttgtaattg
     3901 aacatggtag tgtttcagat taccttatgg ggattgcttc agttgctatt ttgtttgctg
     3961 ttcgtaaata tcttttcacc cattttgatg ggtcaagcaa cattgtttta cgtgctaata
     4021 acaaagtcaa aaaagctaat attttagcta ggattcaact accttgtcaa cccaatgaaa
     4081 ctttaggaga cttacttgtt cgtcatttaa atgatgagga aaaggacgtt gctattggtt
     4141 cagtagtcta tattaataac gttgcattgc gtgttgatag tatgcacgat ggtcttgtga
     4201 cgcgtgtaga aatcattagg agtctacatt aaaaaatata aaaaatcatg tatcttagga
     4261 tacatgattt ttttgtaaat taggttcagc acaattttgc acacttaagt gtgtacttct
     4321 tggtcttttt taaaacgctt acaaaaaata taataaagat acaaaaaaac attacttaaa
     4381 taaagagagg tctttagtta tggaaaactt cctagctccc cttaattggt tctcgcaaaa
     4441 tatcttgcag aatccagcct tcttcgtagg tctattggta ctcattgggt atcttttatt
     4501 gaagaaacca cttcatgatg tctttgctgg ttttatcaaa gcgaccgttg gatatttgat
     4561 tttgaacgta ggtgcaggag gattggttaa tacattccgt ccaatcttag ttgctcttgc
     4621 taaaaaattt aatttagaag ctgctgttat tgacccttac tttggtttag catcagcaaa
     4681 tgctaagtta gaaacaatgg gctttatcag tgttgcaaca acagcactct tgattggttt
     4741 tgggattaac attctcttgg ttgctcttcg taaagtaact aaagtccgta ctctttttat
     4801 tactggtcat atcatggtac aacaagctgc aacaatttca gtatttgtac tgctcttgat
     4861 tccacaactt cgtaatggtt ttggtgcatg ggctgttggt atcatttgtg gtctatattg
     4921 ggcggtaagt tctaatatga ccgttgaagc aactcaacgc cttacaggtg gtggtggatt
     4981 tgccattgga caccaacaac aatttgcaat ttggtttgtt gataaggttg cgccattctt
     5041 cggtaaaaaa gaagaaaacc ttgataactt aaaattacca acattcttaa atattttcca
     5101 tgatacagtt gttgcatcag caacattgat gctagttttc tttggaggaa tcttagctgt
     5161 ccttggtcca gacattatgt ctaatgttaa attaattgga cctggggcat ttgtaccaac
     5221 taagcaagct ttcttcatgt acatccttca aacatcattg acattctcag tttacctttt
     5281 cattttgatg caaggtgtac gcatgttcgt tacagaattg acaaatgcct tccaaggcat
     5341 ctcaaacaaa cttcttccag gatcattccc agcggttgac gttgcagctt catatggttt
     5401 tggttcttca aatgcagttc tttcaggatt tgcttttggt ttgattggtc aattaattac
     5461 aatcgctcta cttgttgtct tcaaaaatcc aatcttgatt atcacaggat tcgttccagt
     5521 attctttgat aatgcggcaa tcgctgttta tgcagataaa cgtggtggct ggaaagcagc
     5581 agtagctcta tcatttatct caggtattat tcaggtggca cttggtgctg ttgctgtagg
     5641 tcttttagga cttgcaggtg gttatcatgg aaatattgac tttgaattcc catggttagc
     5701 atttggttac atcttcaagt atttaggtat cgctggttac gtgattgtat gtctcttctt
     5761 cttagctatt ccacaacttc aatttatgaa atctaaagat aaagaagcat actatcgtgg
     5821 tgatgcgcct gcagaataat agaaatttat aacggaggaa ataaaaatgg ttaaagttct
     5881 tacagcatgc ggaaacggca tgggatcatc aatggttatc aaaatgaaag ttgaaaatgc
     5941 gcttcgtcaa ttaggcgtta gcaactttga gtcagcatct tgctcagtcg gagaagcaaa
     6001 aggtttagct gcgaactacg atatcgtggt tgcttcaaac caccttatcc atgaattaga
     6061 cggacgtaca aaaggtcatt tagtaggtct tgataacctt atggatgaca atgaaataaa
     6121 aacaaaacta caagaaattt tgtaagttaa tagacaaagg gagtgagaca aaagtttctc
     6181 tcccatatct ttagactttt aaggagaaat catgaattta aaacaagcct ttatcgaaaa
     6241 tgactcaatt cgtcttaaat tatctgcaag cgattggaaa gaagcaatca aattatcaat
     6301 tgatccattg attgaaagtg gtgctgttga tgcagaatat tacgatgcca ttattgaatc
     6361 tacagaggaa tttggtccgt actacattct tatgccgggt atggcgatgc ctcatgctcg
     6421 tccagaagca ggcgtaaaac gtgatgcatt ttcattaatt acattaacag agtcagtagt
     6481 ctttccagat ggcaaagaag tctcagtatt gttagcactt gctgcaacaa gttcagctat
     6541 tcatacttct gttgctatcc ctcaaattat tgcccttttt gaattggaaa attcaattca
     6601 gcgcttgacg gaatgccaag aagcaaaaga agtccttgca atggtagagg aatcaaaaaa
     6661 cagcccatat ttagaaggtt tagatttaga aagctaatca ataaactgat attaatagaa
     6721 aggataggac ctataaaaaa tctctgtgat aagaaactct ttgccacaaa taaaatgagt
     6781 atttttatca ggtccttaga ataaacaaca tgacaaaacg tttaccaaat ttacaagttg
     6841 cacttgacca ctcagattta caaggagcta tcaaagctgc cgtatcagta ggacatgaag
     6901 ttgatgttat tgaagctgga actgtttgcc ttcttcaagt aggtagtgaa ttagttgagg
     6961 tccttcgcag tctcttccct gacaaaataa ttgttgctga tacaaaatgt gctgatgctg
     7021 gtgggactgt tgctaaaaat aatgctgttc gcggtgcaga ctggatgact tgtatctgtt
     7081 gtgctactat tccaacaatg gaagcagcct taaaagctat caaagaagaa cgcggcgatc
     7141 gtggagaaat tcaaatcgaa ctatatggtg attggactta tgagcaagcg caacaatggt
     7201 tggatgcagg tatttcacaa gccatttatc accaatctcg tgatgcgctt cttgctggcg
     7261 aaacatgggg agaaaaagat cttaataagg ttaaaaaatt gattgatatg ggcttccgag
     7321 tttctgtaac tggtggttta tcaactgata ctcttcaatt atttgaaggt gttgatgttt
     7381 ttacttttat tgcgggtcgt ggtattacag aggcagatga tccagccgca gcagcacgtg
     7441 catttaagga tgaaattaaa cgtatttggg ggtaatgtat gacacgtcca ataggaattt
     7501 acgagaaggc aacaccaaaa cattttaact ggttagaacg cctacaattt gctaaagaat
     7561 tgggatttga ttttgtagaa ttatccattg atgagtcaga tgaacgtttg gcacgtttgg
     7621 agtggtcaaa agaagaacga cttgagttgg tcaaagctat ttttgagacc ggagttcgtg
     7681 ttccaacaat tacatttagt ggtcatcgtc gtttcccaat gggatctaat gatcctgaga
     7741 aagaagcgcg tgcgatggat atgatgaaaa aatgtatcgt ctttgctcaa gatattggga
     7801 ttcgtaatat tcaattagca ggatatgatg tgtactatga agaaaaatct ccagagacac
     7861 gtgcacgctt tattaagaat cttcgtcaag cttgtacttg ggccgaagaa gcacaagtta
     7921 ttctgtctat tgagattatg gatgacccat ttatgaattc cattgaaaaa tatttggctg
     7981 tggaaaaaga gattgattct ccttatctct ttgtctaccc agatactgga aatgtatcag
     8041 catggcataa tgatctttgg agtgaatttt ataatggaca tcgctcaatt gcagcgcttc
     8101 atattaaaga tacctatgct gttaccgaaa catctaaagg tcaattccgt gatgttccat
     8161 ttggtcaagg gtgtgttgat tgggaagaaa tgtttgctgt cattaagaaa acaaattata
     8221 atggtccatt cttgattgaa atgtggtcgg agaactgtga gactgtagag gaaacacgtg
     8281 cagccattaa agaggcgcaa gattttcttt acccattaat ggagaaagca ggagtgaaat
     8341 aatatggcaa aatcattgca agaaatgcgt gaacgtgtgt gtgaagctaa taaatcacta
     8401 ccagttcatg gtttagttaa atttacttgg ggtaacgttt ctgaagttga ccgcgaagca
     8461 ggattgattg ttatcaaacc ttcaggagtt gactatgacc aattgacacc tgaaaatatg
     8521 gttgtcacag acttagaggg aaatatagtg gagggggatt tgaatccatc atctgatctt
     8581 ccaactcatg ttcaacttta taaagcatgg ccagaagtcg gtggtattgt tcatactcat
     8641 tcaacagaag cagttggctg ggcacaagcg ggtcgcgata ttccatttta tggaacaaca
     8701 catgcagatt atttttatgg gccagtacca tgtgctaggt cacttagtga agatgaagtt
     8761 aacactgctt atgaaaaaga aacaggtagt gttattatag aggaatttga acgtcggaat
     8821 ttagatccaa tggctgttcc tggtattatt gtacgtaatc atggaccatt tacttggggg
     8881 aaagatccag cacaagcagt ttatcactca gtcgttcttg aagaagttgc caagatgaat
     8941 cgtttcactg aacaaattaa tccacgtgtt gaaccagctc caaaatatat tatggataaa
     9001 cactatttac gtaagcatgg accaaatgct tattatggtc aaaaaggaca agaacattaa
     9061 ggagaataat atgaaatact ttttagatac tgccgatgtt tctgaaattc gtcgtcttaa
     9121 tcgtttagga atcgttgatg gtgttactac caatccgact attatttcac gtgaggggcg
     9181 tgactttaaa gaagttatta acgaaatttg ccaaatcgtt gatggtccag ttagtgcaga
     9241 agtgacaggt ttaacttgtg atgaaatggt taccgaagct agagaaatcg ctaagtggtc
     9301 accaaatgtt gttgttaaaa ttccaatgac tgaagaaggt ttagcagcag tttcacaatt
     9361 atcaaaagaa ggtataaaga caaatgtgac attaatattt actgtggctc aaggtctttc
     9421 ggcaatgaaa gctggtgcaa cctttattag tccttttgtg ggtcgtcttg aagatattgg
     9481 aacagatgct tatgctttga ttcgtgacct tcgtcacatt attgattttt atggtttcca
     9541 atctgagatt attgctgcta gcattcgagg attggcgcat gtggagggcg tcgctaaatg
     9601 cggtgcgcac attgccacta ttccagataa aacatttgca tcactattta ctcacccgct
     9661 tactgataaa gggattgaaa ctttcttaaa agattgggat agtttcaaga agaagtagtg
     9721 ttacgataaa gcaaactctt aattatacta gtgcaagaca aactttgata atgggtttgt
     9781 ctttttttat aatcttctcc aataagaaca cttattttca tttttcttat taaatgatat
     9841 aatacgggta aattaagtct aattagaata aggaaataga gatgtttgag aaggtaacca
     9901 ttagtgatat tgcaaattta gtaggtgtgt caaaagctac agtttcctat tatctcaatg
     9961 ggaactataa gaaaatgtct ctgcaaacga aagaaaaaat acgtttagct attaaggaga
    10021 ctggttatca accaagtaag attgctcaaa gtttagttac aaaaaatacc cgtactattg
    10081 gggtcgttat tgctgatatt actaatccct ttatttcgtc tgtgatgaag ggaatacatg
    10141 atacttgtca acaatttggt tactcggtta actttactaa ctcggataat gatattgaca
    10201 ttgagttgga aaatttaaac cgtttaaatc agcaaaacgt ttcaggaatt attttagatt
    10261 ctgtagatcc taatcattca tttatagaaa ctttgagtaa tgatcgtctt gtcatggttg
    10321 atagacaagc taaagatatt aaggtagata ctgtagtatc cgacaataaa gaatcgactc
    10381 aaatatttct tgaaaaaatg caagaaactg ggtatcatga catctatttt gtcacttatc
    10441 ccattgaagg aatttcaacc cgtgagcttc gttacgaagg ttttaaagaa gtggtcagtt
    10501 caaatcctga taagttgatt attatcactg aagatggtag tacacagaga attcttgata
    10561 ttattgaaca ttcagagcaa aaaccagggt tcttaatgat gaacggtcca actcttttga
    10621 attttatgaa aaaattaaat caatcaacgg tcagctaccc tgaagattat ggtcttggga
    10681 gttatgaaga tttagaatgg atgcaggtac ttacaccaaa cgtttcctgt ataaaacagg
    10741 attcatatgg cattggttgc ctggctgccc aatgtttaat tgaaaaaatt agtcaaggaa
    10801 acgagccaac tactgcaagg ttactagaag taaaaaatca aatcgttatt cgtcaatctt
    10861 tttaaaaatg tttgcgagag caagcatttt tttataaaaa agcaaaaaac actatgtaaa
    10921 cgcttgcttt tataaaaaat gatagtataa tagaattata aatgagttaa ccgtttaact
    10981 taagaatagg taagctttta acgaaccatc tctaactctc ttaactcaaa tgtttttatt
    11041 tttttgcacc attagttaaa cggttaactc aagttttatc ttcaccaaag gaggatagaa
    11101 tgaagattaa taaattattg tcagacagac ttgaagcttg ttattcagtt tgtcgtttgc
    11161 aaaatcaaga ctatgatttt ttattagtag cttcagaggt tgatcaagcc tgctttgctt
    11221 atgatttgaa tagcgattta gcaaaaattg ttgtttggga agatgtggga ggtaccatga
    11281 gtattattcc aattcccgga accatggatt ttttggcaac ccagaaattt tacccagggt
    11341 ttaaggccaa agattgtcag gttgtttatg caagttttca aggagatcat tgggacattt
    11401 gtaacgcatt tgattttccg tatttacatc gctttgatct cattaaaggt aaagaaaatc
    11461 aactttactt tataggttgt acaattgcta acagtaaagc ctacactgag gattggagtg
    11521 ataaaggccg aatttttgtt gctcgtttta atactcaaaa ccatacattg gaaggattgc
    11581 aacaattgcc tcaaacttta ttaaaaaatc atggatacta tgccattcag gatgaaggat
    11641 attcattgat tacttcagtg gaaggggtat tcaaactcac ttatccagaa ttttctacta
    11701 caggcgactg gcaattagaa cggcttttcg atgaggagac aagcgatgtg gtgaaagtgg
    11761 atattaatca ggatggcaag gatgagtata tgattatcca aggttttcat ggagatcgtt
    11821 tacgtatctt cactgaagat ttcggtcgag aattattcca ttatccagaa aaaaccccat
    11881 ttggtcacgc tatttggagt ggtcgtttac ttaatcagac ttgttttgta ttcgggtggc
    11941 gatcagaaaa agcagaatta aggctttttc actttgtaga tgggcacttg gtttcagaat
    12001 tagtagatgc aaaagcagct tctagtaatg tcttagcttt tgaaaaagat ggaaaagctt
    12061 atcttttctc agccaataac ggacgtggcg aagttgctct ttatcaatta gtaaaataaa
    12121 tttaagaagg agacaatatg gacaagaaaa aaatcttagt aacgggtatt gtgcctaaag
    12181 aaggtctaag aaagcttatg gaccgatttg atgttactta ttcagaagat cgcccatttt
    12241 cacgtgacta tgtgttagag catttatctg aatatgacgg atggttactc atgggacaaa
    12301 aaggcgataa agagatgatt gatgcaggtg aaaacttaca aattatttct ttaaatgcgg
    12361 ttggttttga tcatgtagat actgcgtatg ctaaagaaaa aggaattgta gtctctaatt
    12421 cccctcaagc agtacgagtt ccgacagcgg aaatgacttt tgctttaatt ttagctgcca
    12481 gtaagcgcct tgcattttat gatagtattg taagaagtgg agagtggatt gatccgagcg
    12541 aacaacgtta tcaaggtttg actttgcaag gttcaacatt aggtatttac ggaatgggac
    12601 gcatcggttt gacagtcgca aattttgcta aagcatttgg gatgactgtt gtttacaatg
    12661 atgtttatcg acttcctgaa gataaggaaa aagagcttgg tgtaacctat ctcgaatttg
    12721 atcagcttat tgaaacagct gatgtcataa ccatccacgc cccagcatta ccatcaacaa
    12781 tccacaaatt caataaagat gtttttgcta agatgaaaag tcgaagctac ttaatcaacg
    12841 ctgctagagg cccaatcgtt tctgaagaag cacttattga agctctgaaa gaaggtgaaa
    12901 tcgcgggtgc gggattagat gtttttgaga atgaaccgca ggtatctgaa ggattgagaa
    12961 gtcttgataa tgttatcatg agtccacatg ctgggacagg tactattgag ggacgtagaa
    13021 ctttggctga agaagcagct gacaacatca ttgccttctt tgatggcaag ccacaaaata
    13081 ttgtcaataa ataattggct agtgagcgca gagccatctt ttgaaaaaat gggacctccc
    13141 atttttttac gggtaaatgt aagcgcttaa taatttaata aaggtgaact tatgctagaa
    13201 gctttacata aattctttga agtttttggt gcagcaactg ttgtacccgc tatgattttt
    13261 attgtcagcc tttttctaaa ggtcaatcca aggcgagctt tctttagcgc cttacgagct
    13321 ggtgttggac tcacaggctt tggttggatt atatcagctt ttgctcctat ggtgactaag
    13381 tatatccagc agatggttaa aactactggg ctccatttac caattgtaga tattggttgg
    13441 caggctggtt ctctcaccgc cttttcttct gagattggcc tctctttctt tgtttttggc
    13501 ttgctaattg aattggggct tttcttgctc gggattaccc gagtatttgt cccatctaat
    13561 ctttggaata actttggcta tatgatttgg ggtacaatgg cctatgcagc aacaggtaat
    13621 tttatattgt ctttcgcatt tatggttttt gtcttactct attcattagt gatgtcagaa
    13681 gtattggcag atcgatggtc ggagtattac ggtgttaaaa atgcaacgat caactcgatt
    13741 cataatattg aaacccttat tccagctctt attcttgatc ctctttggaa cctacttagt
    13801 gtcaataagg taaagttgaa cccagaatca ttaaagacta agttagggat atttggtgag
    13861 ccaatgacac ttggttttat acttggtgtg attattggtg tattaggtag tttacgtaac
    13921 cttgcaagta ttgatacttg gggaggtata cttggttttg cagttgctct tgcagcggtt
    13981 atgactattt ttcctttgat tacgggggta tttgctagtg cttttgctcc tcttgctgaa
    14041 gcagttgaac gcaataagaa aaaagaaagt caagctgagc aaggagcact ggataaaaaa
    14101 cgttggttta ttgcagtaga tgacggtgtt ggttttggtg aaccagcaac tattatcgca
    14161 ggtctcattt tagtaccaat aatggttgtt atttccttga ttttacctgg aaatgaagct
    14221 ttaccagtcg ttgaccttat tgctatccct tttatgattg aagctatgat agctgtgtca
    14281 aaaggaaata ttcttaaagc tattttgaat ggaatcatat ggttttcact aggtctttat
    14341 gctgctagtg ccctaggtcc aatctacaca gaagctgtta agcattttgg aactgcttta
    14401 ccagcagggg taactttgat tatgagcttt aatctcctag ctcatccttt gacagctcta
    14461 gtcttctttg cttggatatc tgggaaccca ctctatattg gtgtgacagt tgttatttat
    14521 cttgctttct tgttcttgct tcgtactcgt cgtgaatcta tttacgcata ccttaaaact
    14581 atggctgata agaatagcgg atatgaaggt agttgtgaaa aaatctttat tgctgaagat
    14641 taaaaatctg ttggaggaat actcatgaaa aaaattgatg gacatgctca tattgttgat
    14701 acaattgcag gatttaatgg taaaggacgc cttaatgccc ttgggaacgg ttatgctatt
    14761 tgggatgatg gtgatttaat taagctcatc ccagacgaat acggtgacaa agcatttaca
    14821 gctgaagctt ttttgaagta tatggatgct aatggtgtag ataaagcagt cattttgcaa
    14881 ggtcatctta atggctatca aaattattat acgcatttag ctattaaacg ttatccagaa
    14941 cgttttacag gcgcattctc tgttgatcca tttgcagata atgcaatgca aattgtaaaa
    15001 cgtcatgttg aagtccttgg ttttcgagct attaaatttg aaattagtca aggagggggt
    15061 atccatggat accgtggaca aaaaacgcct ttccgtcttg atacagaccc acatgtgagt
    15121 cgtattttga cttatctgtt agactatcca ggatttgtag ttacagttga ttatggcaat
    15181 tgggatcaaa ttagtcacca acctgatgct attgctaatt tagctcgcct ttatacgagt
    15241 ctcgattttg ttgtttgtca cctctctttt ccacatgttg agcattcaaa tcgattgcgt
    15301 gctgaactta atatgtggaa aaacttcaac aatatctata ctgatatttc agctattcaa
    15361 gatattgatg ccccagatag ttttcctttt ccaaaatcag aacaaaatgt acgtattgct
    15421 aaagaagttc ttggggctaa gcgtatcatt tggggaactg attctccatg gtcagcgacc
    15481 ttcaatactt acgaagaatt agctacttgg ctagagaatg ttgatatctt cagtcaagag
    15541 gaactagaag atgttatgta caataatgct gagcgtgttt actttaaaga agaacatgta
    15601 aaggctaaca gagcaggtat agatgataca accaaagagc tcaatctcta ttagaagagt
    15661 tttcaaggta caagagtttc ttgtacctcc tttttaaatc aacaatgagg agacaaatta
    15721 tgacttacta ccttagcatt gactacggcg gtacaaatac caaggcgctt atttttgaca
    15781 aattaggaca ccaaatcgct gtttcgagtt ttgaaacttt aaaaaatgag gctcaatctg
    15841 gtcatcgtca agtaaacctt gttaaaacgt ggaatgctat aacttctgct attagagagg
    15901 ttattcaaat ctcaaaactc agccctgagc agattagtgc agtagcatgt attggacatg
    15961 ggaaaggtct ttatctgcta gataataagt tggagccact tgaacaagga attttgtcta
    16021 cagataatcg tgccaaagat ttggcgcaat atttcgaatc taaacttgat aatatttggg
    16081 agttgactcg gcaacatatt ttcccttcac aaagtccagt tattttacgt tggcttaaag
    16141 attatcagcc cgaaacctat aaatcaatag gggcggtcct ttctgcaaag gactttattc
    16201 ggtataagct tacaggaaaa gtacagcaag aatatggtga cgcttcaggt aatcattgga
    16261 taaatttcca aacaggaact tatgatccag ctattttaga tttttttggc attagagaga
    16321 tagaaaactc acttcctgaa cttatagata gtgcagattt agttcctggg ggaattagtt
    16381 ctcaagcagc aaaagagact ggtcttgtag aagggacccc cgttgttgga gggctctttg
    16441 acatcgatgc ttgtgctctt ggatcaggtg ttttagagtc agatactttt agtgttattt
    16501 cgggaacttg gaatattaat acttatccaa gtttaaaacc agcaaagcaa gatagtggtc
    16561 ttatgacttc ctattttcca gatcgtcgtt atctcttaga ggcaagtagc cctacttctg
    16621 cagggaatct tgattttatg ttaaaaatgc tcatgcatca agaaattgat aacgctaaat
    16681 ctagtggagg ttctatctat gataatttag aagaatttct cactcatact gatgctacac
    16741 atcatggact tattttcttt ccgtttcttt acggtagtaa cacatcacaa gatgctagcg
    16801 cttgcttttt tgggctaaca actaaatcga cgaaatctca gatgatacgt gcggtatatg
    16861 aaggtattgc gtttgcacat aagcagcata tcactgattt aataaaaagt aggggcggtg
    16921 tgccaaaaat aattcgtttc tctggcggag ctaccaactc accagcatgg atgcaaatgt
    16981 tttctgatat cttaaacctt cctattgaaa cagtagaagg cacagaatta ggaggattag
    17041 gaggagctat tttagcacat catgctttag ataagatttc gttaaaggaa gcagtccaag
    17101 atatggttcg tgtaaaagct atttataaac ctcaattatc cgaagtaaag gggtacgaaa
    17161 aaaaatatca cgcttaccaa aaattattag aaacactgga tcctatttgg tcggaactcg
    17221 gtcatctgaa taagtaggga tatcaaagga gttgttatga aacaagaaga tattattatc
    17281 aatagtattg tattcaaaaa acagttagac aaaggtttta cacaagttca gctcattaag
    17341 ttagtaaagg atttaggatt ttcacgattt gagatacgtc aagaattgct tcaagatcca
    17401 gatagagagc tacccgcttt aaaagcagag gctgactttt atgatatcaa tctttactat
    17461 agtgctaatg aagaccttat caaaggaggg aaagttaatc cttatcttaa taaaggcctt
    17521 aaagaagcat cacagttagg agctcctttc attaagctaa atgttggaca aaccagaaac
    17581 ttaagtaaag aagagttaga acctcttaaa gagatattga aaagccaaac tattggtata
    17641 aaggtggaaa ataatcaaga ccctaaagca gctacagtgg agaattgcca atattttatg
    17701 actctagtga aagaattaca aataccaata agttttgttt ttgatactgc taattgggcc
    17761 tttatcaacc aagatctata ccaagcggta aatagcttag cttgtgatac cacctacctt
    17821 cactgtaaaa attttttaca ggtagctgga aagcctcacc tctcgaagag cctcttcgaa
    17881 ggagagattg atctgactga tttgttaaag agtttctcta attgtgagta tctagctcta
    17941 gaatacccca cagagttgga ggtattaaaa agggatgttc aacgtttaat ctctatcagc
    18001 aatagtcaaa acttttaatg aaaaggcttt aaaaagatgt tataataagt ctgtatgaat
    18061 tcgagtagaa aagaggtata ttcttgatta ttctcgataa aaaaagttat gacctcctct
    18121 tttacctatt gaaattagag gaacctgaaa cagttatggc aattgccaac gcacttaatc
    18181 agtctaggcg taaagtgtat tatcacttag agaagataaa cgatgcactg cctagcgatg
    18241 tgcctcagat tgttagttat ccacgtgtag gaatcttgct aacagaaaaa caaaaagcag
    18301 cctgtcgtct tttattagat gaagtgactg attatagtta cgtcatgaaa agtagtgaga
    18361 ggttgcagct gtctttagta tctatcgtgg tagctaagga ccgcataacg attgataggt
    18421 tgatgcaact aaacgatgtt tctcgaaata ctatcttaaa cgatttaaac gaattaagaa
    18481 gtgagttagc agagaaagaa tataatttac agttacaatc aacaaaatgt cgtggttatt
    18541 ttttagatgg tcacccattg tccattatcc agtacttata taagctctta gatgatatct
    18601 accataatgg aagtagtagt tttatagacc tttttaatca taaactgtct caagcttttg
    18661 gtgccagcac atatttttct aaagaggttc ttgattattt tcaccattat ctcttcattt
    18721 ctcaacgaag tctaggtaag aaaatcaaca gtcaagatgg tcagtttatg attcagattt
    18781 tgccttttat actaatggct tatcgtaaga tgcgattaag tcctgaagta cagacctctc
    18841 ttaatagtga ttttagcttg gtttggcaac gtaaggaata tgagattgct aaagagttgg
    18901 ctgatgagct ggaagaaaat tttcagttat cactggatga gattgaagtg ggactagtag
    18961 ccatgcttat gcttagtttc cgcaaggacc gtgacaatca tttagagagt caggattatg
    19021 atgatatgcg agctactcta accagttttt tgaaagaatt ggaagaacga tatcaccttc
    19081 actttgttca taaagaggac ttactaagac aacttcttac tcactgcaag gcactcttat
    19141 atcgtaaacg ttatggtatt ttttctgtta atcctttaac agagcatatt aaagacaaat
    19201 atgaagaact ttttgccata acctcgtctt ctgtaaagct tttagagaaa gcttggcaaa
    19261 tcaaattgac cgatgatgat gtagcatatc taacgattca tttaggaggg gaacttcgta
    19321 atagtcaaca atctcctaat aaacttaagt tagttattgt atctgatgaa ggaatagcga
    19381 ttcagaaact tcttttaaag caatgtcaac gctacttaac aaacagtgat atagaagctg
    19441 tttttacaac cgaacagtat caaagtgtga gtgatcttat gcatgtagat atggttgtct
    19501 ctactagtga cgctttagaa tctcgttttc cgatgttagt agttcaccct gttttgacag
    19561 atgatgatat tattcgcttg attcgctttt ctaaaaaagg taactgtgca aatagtaatc
    19621 aatttaccaa tgaacttgaa aaaacgattg ctcaatatgt caaggaagat agtgaacgct
    19681 acgtgctgaa atctaagatt gagaaactta ttcatcaaga attgctccaa gacgtccttc
    19741 cccttcaaag tacagtttgt taaaatataa ttttatggta gcctctcagc ttttctgaag
    19801 acagaaaaag aagctggggc ttttttggtg cttttaaaca atgtgctaga tatttaatcg
    19861 caatgagttc ctctatgaca tgataattaa agttgagaaa gctagtactt ttaatgggag
    19921 tactttttat ttgtgcttat tttaattaaa attatgagtc taatttggaa cagacagccc
    19981 agttttgaac acataaatgt gtgacacttg gtcttttttt caaaagtatt tgaacttata
    20041 ctagtagatg taagaaacaa ttaatcagga ggaaataaaa tgcctaacgt taaagatatt
    20101 actagagaat catggatttt atcaacattc ccagaatggg gaacatggct aaacgaagaa
    20161 attgaagaag aagttgttgc tgagggcaac tttgcaatgt ggtggttagg aaactgtggt
    20221 gtttggatta aaacaccagg tggtgctaat gttgttatgg acctatggtc aaaccgcggt
    20281 aaatcaacaa aaaaagtaaa agatatggtt cgtggacacc aaatggctaa tatggcaggt
    20341 gttcgtaaac tccaaccaaa cttacgcgca caaccaatgg ttattgatcc atttgctatt
    20401 aatgaattgg attactatct tgtttcacat ttccacagtg accacattga tatcaacact
    20461 gcggcagcca ttattaataa tcctaactta gaccatgtta aatttgttgg gccatatgaa
    20521 tgtggtgaaa tttggaaaaa atggggagtt ccagaagaac gaatcatcgt gattaaacca
    20581 ggtgaatcat ttgagtttaa agacatcaaa gttactgctg ttgaatcatt tgaccgcact
    20641 tgcttagtga cacttccagt agatggtgca gaagaacatg atggagaact tgctggttta
    20701 gccgtgactg atgaagaaat ggctcgtaaa gcagttaact atatttttga aacaccaggt
    20761 ggaacaatct atcatggagc tgactcacat ttttcaaatt attttgctaa acatggtaaa
    20821 gactacaaga ttgacgtagc tattaacaac tatggtgata acccagttgg tattcaggat
    20881 aaaatgactt caatcgatct tcttcgtatg gcagaaaatc tccgtgccaa agtcattatt
    20941 cctgtacact acgatatctg gtctaacttc atggcttcta cagacgaaat cttacaattg
    21001 tggaaaatgc gtaaagaacg ccttcaatat gacttccatc catttatttg ggaagttgga
    21061 ggtaaataca cttatccaca agataaagat agaattgaat accatcaccc acgtggtttt
    21121 gatgattgct tcgaacaaga atcaaatatt caatttaaag cacttctata atggctaatg
    21181 agagactgta tgtcgcatga tatgcagttt ttcaatttta aaatatgtta ctaaaatcac
    21241 aattaagatt aggagaaata atgtcagaat ttgatacaaa atcatattta gaaaaacttg
    21301 atgcatggtg gagagcagct aattatattt ctgcagcaca aatgtatcta aaggataacc
    21361 ctctcttgag acgagagctg gttgaaaacg acttaaaggt tcatccaatt ggtcactggg
    21421 gcactgtacc tggacaaaat tttatctatg ctcacttaaa tcgcgctatt aataaatacg
    21481 atttagacat gttctatatt gaaggtccag gtcatggtgg tcaagttatg gtatctaatt
    21541 catatttaga tggttcatat actgaattaa accccaatat tgagcaaaca gaggatggct
    21601 ttaagcagtt atgtaaaatc ttctctttcc caggtggaat tgcatcccat gcagcaccag
    21661 aaacaccagg gtcaattcat gaaggcggtg aacttggtta cgcactttct catgcgacag
    21721 gtgccatcct ggacaatcca gatgttattg ctgcgactgt tattggtgat ggtgaaggtg
    21781 aaacaggacc acttatggct ggttggctat ctaatacctt tatcaatcca gtaaatgatg
    21841 gtgctgttct accaatcttc tatttaaatg gtggtaaaat ccataaccca actatctttg
    21901 aacgtaagac agatgaagaa ttatctcagt tttttgaagg attaggttgg aaacctattt
    21961 ttgcagatgt tgttgaactc tctgaggatc atgcggctgc tcatgctttg tttgcagaaa
    22021 aattagatca agctattcaa gagattaaaa ccattcaatc agaagcacga caaaaaccag
    22081 cagaagaagc tatccaagca aaattccctg tcttagttgc acgtattcct aagggttgga
    22141 ctggtccaaa agcttgggaa ggaacaccaa ttgaaggtgg cttccgcgct caccaagtac
    22201 caatttcagt agatgcccac catatggaac atgttgattc tcttttgtca tggcttcaat
    22261 cataccgtcc agaagaatta tttgatgaaa atggtaaaat cgttgatgag attgctgcta
    22321 tttcaccaaa aggtgatcgt cgcatgtcca tgaatccaat aaccaatgct ggtattgtta
    22381 aagcaatgga tacagcagat tggaagaaat ttgctcttga tattaatgtt ccaggtcaaa
    22441 ttatggcaca agatatgatt gaatttggaa aatatgcagc agatttagta gatgctaacc
    22501 cagataattt ccgtattttt ggtccagatg aaacgaaatc aaatcgtctt caagaagtgt
    22561 ttacacgtac tagccgtcaa tggcttggtc gtcgtaaacc agattatgac gaagctttaa
    22621 gtccagctgg acgtgttatt gattcccaat tgtctgaaca tcaagcagaa ggtttcttag
    22681 aaggttatgt tttaactggt cgtcacggct tctttgcttc atacgaatca ttccttcgcg
    22741 ttgtagattc aatggtaaca caacatttca aatggttacg taaatcaaaa acacacacaa
    22801 catggcgtaa aaattatcca gcgcttaacc taattgcagc ttcaactgtt ttccaacaag
    22861 atcataatgg ttatactcac caagatccag gtattttaac tcacttagct gaaaaaacac
    22921 ctgaatacat tcgtgaatat ttaccagcag atactaactc acttctagct gttatggata
    22981 aagcgtttaa agctgaagat aaaattaatt taattgtgac atctaagcac cctcgtccac
    23041 aattttactc tattgctgaa gcagaagagt tagtggcaga aggatataag gtgattgact
    23101 gggcttcaaa tgtgtcgctt aatcaagagc cagatgttgt ctttgctgcg gcgggaacag
    23161 aacctaactt agaagctttg gcagcaatta gcattcttca taaggctttc ccagaactta
    23221 agattagatt tgttaatgta ttagacatcc taaaacttcg tcatccttca caagatgctc
    23281 gcggtttatc agatgaagag tttaacaaag tctttaccac agataaacct gttatttttg
    23341 ctttccatgg ttatgaagat atgattcgag atattttctt tagtcgtcac aatcataatt
    23401 tgcatacgca tggttaccgt gaaaatggtg acatcacaac gccatttgat atgcgcgtga
    23461 tgtcagaatt ggaccgattc catctggcac aggatgcagc tcttgcttct ttaggacatg
    23521 aagcgcaagc cttcagtgat gaaatgaatc aaatggttgc ttatcacaaa gactatattc
    23581 gtgaacacgg agacgatatc ccagaagtac aaaattggaa atgggaaaat atcaagtagt
    23641 cactttttca ggcctatact tacaaacaaa aacactttta gattttttct aagggtgttt
    23701 tgttatttta tagaagaatg accgcttttt caaaagcgtt gagtggtcac ttttaaagtt
    23761 gtaatattaa tagtaataag tctttaaata attcaataat gtcataacaa taatggtgtg
    23821 ggctaattaa aatatctcta agcaaaggtg ttgacataaa gagaccatac atgatattat
    23881 agttatgttc tgtttcggca gtttattttt ttgagtaaaa aagtgactac ccttttacga
    23941 taacttaacg tcactttata gataagacac ataaccttaa aaataagata cgaaattttt
    24001 agtaggagat aagaatgaca aatattttag aagtgaagaa tttaaccaaa atttttggga
    24061 aaaagcaaaa agctgctctt gaaatggtta agcaaggaaa aagtaagaca gaaattcttg
    24121 aaaaaacagg agcgactgta ggtgtctatg atgcgagttt tgaaataaaa gaaggcgaaa
    24181 ttttcgttat catgggactt tctggttcag gaaaatcaac tttagttcgg atgttaaacc
    24241 gtttgattga cccatcatca ggaaatatct atttagatgg taaagatatt gcaaagatga
    24301 atgtagaaga tttacgtaac attcgtcgtc acgatatcaa catggttttc caaaattttg
    24361 gtcttttccc acatcgtaca attttagaaa atacagagtt tgggttagag atgcgtggtg
    24421 tttctaagga agaacgtaca acgttagctg aaaaagcttt ggacaatgca ggtctattac
    24481 cgtttaaaga tcaatacccc agtcaattat caggaggaat gcaacagcgt gttggtttag
    24541 cacgtgccct tgctaacagc cctaaaatcc ttctgatgga tgaggccttc tcagctttag
    24601 acccattaat tcgtcgtgaa atgcaagatg agcttcttga tcttcaagac acgaataaac
    24661 aaactattat ttttatcagc catgatttga atgaagcgtt acgtattggt gatcgtattg
    24721 ctcttatgaa agatggagaa attatgcaga ttggtacagg agaagagatt ttgactaatc
    24781 cggctaatga ttttgttcgt gagtttgttg aagacgttga tcgctcaaaa gtgttaactg
    24841 ctcaaaatat tatgataaaa cctttaacaa ctgttttaga aattgatggc ccacaagttg
    24901 ctttaacaag aatgcatcgt gaggaagtct caatgttaat ggcaacgaat cgtcgtcgcc
    24961 aacttctggg atccctaacc gcagatgctg caattgaagc tcgtaagaaa ggtttgccgc
    25021 tttctgaagt gattgataaa gatgttgtaa cggtctcaaa agatacagtt attacagaca
    25081 tcatgccttt aatttatgat tcatctgctc cgatagccgt tactgatgat aatgatagac
    25141 tcctaggggt tatcattaga ggacgtgtga tcgaagcact tgctaacgtt caagatgaaa
    25201 cagtagtaga atcgccaaaa gaaacggtag aagcttaata gagaggacaa atcattttgg
    25261 aaaatttatt acaacataag ttaccggtcg caccatttgt agagtcagcg accaattgga
    25321 ttacgaaaac tttttctggt ttatttgact ttatccagac aatcggtaat gcattgatgg
    25381 attggatgac aaaaacatta ctattcatta atcctttact ttttattgta ctaattacag
    25441 ctgctgtatt tttcttagct aagaaaaaat ggcaattacc gacatttact ttcattggtt
    25501 tgctatttat ctataaccaa gggctgtggg agcagttgat taacactttt aacttggttt
    25561 tagtagcaag tttaatttct attattattg gtgtaccact aggtatttgg atggcaaaaa
    25621 gtgataaagt taaacaagtt gttaatccaa ttcttgactt tatgcaaact atgccagcat
    25681 tcgtttacct tattcctgca gtagccttct ttggtatcgg tatggtacca ggtgtctttg
    25741 cctctgttgt atttgctttg cctccaacag tgcgctttac taacttggct attcgtgaaa
    25801 tcccacttga gttaatagaa gcatctgatt catttggttc aacagctaag caaaaattat
    25861 tcaaagttga actaccactt gctaagaaca ctattatggc aggaatcaat caaactatga
    25921 tgttggctct ttctatggtg gttacaggat ctatgatcgg tgcacctgga cttggtcgtg
    25981 aagtgttatc ggcgcttcaa catgctgaca ttggtacagg gtttgttagt ggtctatcgc
    26041 tagttatttt agcaatcgtg ttagaccgtg ttagtcaatt ctttaattct aaaccgggtg
    26101 agaagcaagc taaaacatca aaagttaaaa aatgggtagg tttaggggcc ctggctctct
    26161 tcattctagc agctcttggt cgcattgttg tcaatatgac ttcaggaaat gaagccaaag
    26221 gtcaaaaagt gaaaatagct tatgtccaat gggattcaga agttgcttca accaacgtta
    26281 ttgctgaagt tttaaaatct aaaggatatg atgttgaatt gacaccactt gataatgcag
    26341 tcatgtggca aacagttgcc aatgggaatg ctgattttac aacaagcgct tggttaccaa
    26401 agacccacgg gcaatatttt aataaataca aaaatagttt agatgactta ggtcctcacg
    26461 ttgagaacgt caaaataggt ttagttgttc ctaaatacat gaacgtaaat agtattgaag
    26521 aattatcaaa tcaagcagat aaacaaatta caggtatcga accaggtgca ggtattatga
    26581 agtctgctaa acaatccctt aaagattatc caaatctttc aagttggaag ttgttatcag
    26641 catcaacagg agctatgaca acaaccttag ggaaggctat caaaaacaaa gatcaagtgg
    26701 tcattacagg ttggtcacct cactggatgt ttgctaagta tgatttaaaa taccttaaag
    26761 atcctaagaa atcatttggt ggtgaagaac atatcaatac aatagcacgc aagaatttga
    26821 aaaaagatat gccaaaagta tataaaatta tcgataagtt caagtggact aaagaagata
    26881 tgga
//
LOCUS       DATRNK010000022        26790 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.022, whole genome shotgun sequence.
ACCESSION   DATRNK010000022 DATRNK010000000
VERSION     DATRNK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26790
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.022"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..1936
                     /locus_tag="VBC52_000954"
     CDS             <1..1936
                     /locus_tag="VBC52_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEO6882551.1"
                     /translation="LSFQADASGNTYQAFYTLKNKSEKDYFQNLDNNIMIIADIKLEE
                     AEERRHFNGFDYRQYLKRHGIYRIAKVTKIKQIRLFQHRSFFALMSKWRRSAIVISQT
                     FPNPMRHYMSGLLFGYLDKTFDDMSDLYSSLGIIHLFALSGMQVGFFLGIFRYICLRI
                     GLRLDHVWLLQIPFSLIYAGLTGFSISVVRALIQSLLSHSGVKKDENFALCLLICLIS
                     LPHSLLTTGGVLSFAYAFILTMTSFDHFSSIKKVAIESLTVSVGILPILTYYFSGFQP
                     ISIILTALLSFAFDIIFLPLLTVIFVLSPIVKLSCINSLFEILEVLLKWTGQLFPRPL
                     IFGKPSLFLLIVMIIILGLLYDYYHSKCFRYCSLLIIFTLFFITKNPITNEVAILDVG
                     QGDSILVRDWLGKTILIDTGGRVRFEQPEEWKQKVNQSNAKRTLIPYLKSRGISKIDD
                     LVITHTDTDHMGDMEVISKHFKVARLITSSGSLTNSQYVKHLSKIGVAVKSIEAGDKL
                     AVMGSYLQVLYPWHKGDGKNNDSIVLYGHLLGKGFLFTGDLEEEGEKQLLEAYPNLSV
                     DILKAGHHGSKGSSSLSFLKKLSPSVVLVSAGKNNRYQHPHQETLQRFQKIKSKIFRT
                     DQSGTIRLTGWWKWHIQTVR"
     gene            2062..2871
                     /locus_tag="VBC52_000955"
     CDS             2062..2871
                     /locus_tag="VBC52_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6882552.1"
                     /translation="MTIKRIFCDMDGTLLNSEGQVSKSNATLIREAAIPVTLVSARAP
                     MEMKDAVDALQLGGVQVAFNGGLIYRIGDNNQVLPIHTQIIKKSTVKQLLRGIRFHFP
                     QVSLSYYDLNNWYCDKIDEGIRYEHSLTQQCPTFIHNEDQFLEGHTNTFKIMMITFDE
                     ANMLELEKYLQSLELPEITIQRSGKAYLEITHLLAKKSKGIAYILQKEQLAREETAAF
                     GDGHNDLPMLEMVGYPIVMDNAFDDIKAIAYQLTKSNDEDGVGYGIQKFLK"
     gene            2882..3826
                     /locus_tag="VBC52_000956"
     CDS             2882..3826
                     /locus_tag="VBC52_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6882553.1"
                     /translation="MTSISDIAKKAGVAKSTVSRVINHHPHVSDETRQKVMALITELD
                     YIPNQLARDLSRGKTQKIGVVIPHTRHPYFTQLINGLLDAAKTTDYQLVMMPSDYNQE
                     LELSYLKQLKMEAIDALIFTSRAISLDIIETYAKYGRIVVCEKLQEYNHLSSAYLDRY
                     SSFLEAFSDMKLRGLEHLVLLFSRNNESSATYQSALLAYQEVYGQLSSPYMVVGNVHD
                     FNDGLNLSYQLVKEVSIDGILATSDEVAAGLIKGYEESRKKCPYIIGQECLLVGQLLK
                     LPTIDHKSYYLGKLAFKQALAEKISQEVLFSEFLSQDN"
     gene            complement(3885..4877)
                     /locus_tag="VBC52_000957"
     CDS             complement(3885..4877)
                     /locus_tag="VBC52_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6882554.1"
                     /translation="MKNYRKLIVLLLLTFFVIFMGAYAYTHIVEKRSLTSNTIEKTLP
                     VVNQIKPQTIKEYQNYLTKVAKRNVLPVDIPQALNNEKVEITATDGMQTFTWNDKNNP
                     KQKVIFYVHGGSYIHQASELQYIFVNKLAKKLDAKVVFPIYPKAPTYNYSDAIPKIKK
                     LYQNTLASVTSPKQIILVGESAGGGLALGLADNLVTEHIKQPKEIILISPWLDIATNN
                     PKIEKVQKKDPLLKAWQLQQVAPYWANGKKNFKNPQVSPLYSSQFNKMAPISFFIGTH
                     DIFYPDNQLLHQKLAKENIKHHYIVGQKMNHVYPVLPIPEAETAFKQMLKIINH"
     gene            5053..5781
                     /locus_tag="VBC52_000958"
     CDS             5053..5781
                     /locus_tag="VBC52_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6882555.1"
                     /translation="MFHREDFSKALLKKALQPGMRVMDIGCGSGELTRLAADIVGKEG
                     DVVGIDINEQLLWQARHDNGYANVSYQNADLAALSSFETKSFDAIIGRRVLMYQKDPK
                     KVIESLTPLLKPNGFYVFQESDAMCLELAKPSFVNMNKIQSLIWQTVEKEGGHIHIGS
                     QLEQLMLEAGLIVDHFVSENVIQTSQTGTDLSWLLNIMKERIQKYSDLQGLEDVNLQK
                     ELEENNDHFIRDCQFGIISHRVDE"
     gene            5835..6872
                     /locus_tag="VBC52_000959"
     CDS             5835..6872
                     /locus_tag="VBC52_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEO6882556.1"
                     /translation="MIAIEEIGRITPDNLGLVTVLAGEDLGQYAQMKEKLFQVIGFNK
                     DDLAYSYFDLSEEDYQNAELDLESLPFLSDYKVVIFDQFQDITTDKKTYLDEQAMKRF
                     EAYLQNPVDTTRLVICAPGKLDGKRRLVKLLKRDARVLEANTLKESDLKTYFQKYAHQ
                     EGLVFEAGVFDELLIKSNYDFSDTLTNIAFLKSYKTDGHISSNDVREAIPKSLQDNIF
                     DLTQDVLLGRIDLARDLVRDLRLQGEDEIKLIAIMLGQFRMFLQVKILASKGKSESQI
                     VSELSHYIGRKINPYQVKFAVRDSRNLPLAFLKEAIRILIETDYAIKRGTYDKDYLFD
                     LALLKIAHKKP"
     gene            6952..7560
                     /locus_tag="VBC52_000960"
     CDS             6952..7560
                     /locus_tag="VBC52_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEO6882557.1"
                     /translation="MAIILPDLPYAYDALEPHIDAETMTLHHDKHHATYVANANAALE
                     KHPEIGEDLEALLADVSQIPEDIRQAVINNGGGHLNHALFWELMSPEETQISQELSED
                     INATFGSFEDFKAAFTAAATGRFGSGWAWLVVNAEGKLEVLSTANQDTPIMEGKKPIL
                     GLDVWEHAYYLNYRNVRPNYIKAFFEIINWNKVNELYQAAKA"
     gene            7898..8749
                     /locus_tag="VBC52_000961"
     CDS             7898..8749
                     /locus_tag="VBC52_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEO6882558.1"
                     /translation="MIIKRVLNHNAVISVTHQGLDVLLMGKGIAFKKRIGDRINSDAI
                     EKSFVLKNSDNMNRFTELFITVPEEVVACSERIINLGKIKLGKNLDEILYINLTDHIH
                     SAIERHEQGMVIQNPLRLEIQRYYPDEYSIGMKALELIKDELGICLTIDESAFIAMHF
                     VNAGLDNPFNEAHKITEIVSYIEQKVKIDFRTELDESSIDYYRFMTHTKLFAQRVLSG
                     MKYEDDDADLLLVVKKKYPREYKCVKEIGNNMAIQYQYQLNSSELLYLTVHVKRLVKN
                     LKEISDD"
     gene            8742..10610
                     /locus_tag="VBC52_000962"
     CDS             8742..10610
                     /locus_tag="VBC52_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEO6882559.1"
                     /translation="MTKYQETAKAILAAVGGEKNIQHVTHCVTRLRLVLDNDEIVNDQ
                     VIKTIPNVIGVMRKNDQYQIILGNDVNNYYNAFLALGHFENTTREFSSQKKSSILEKL
                     IETIAGVITPLIPALLGGGMLKVIGILLPMLGIASSSSQTVAFINFFGDAAYYFMPIM
                     IAYSAASRFKVTPVLAATVGGILLHPAFVTMVAEGKPLSLFGAPVTLASYGSSVIPIL
                     IMVFLMQYIERWINKIVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGKGLSSAML
                     SIYHVAPWLALSILGAIMPLVVMTGMHWAFAPIFLAASVATPDVLILPAMLASNLAQG
                     AASLAVAVKAKQKQTRQVAFAAGLSALLAGITEPALYGVTLKFKKPLYAAMISGGLVG
                     AYIGLVNIASYTFVVPSIIGLPQYINPQGGNNFSNAVIAAIATIILTFIITWFLGIDE
                     GENEKSSINAQEHTHIRSGLSKKETLYSPMVGNVLPLSKVPDETFSSKLLGEGLAITP
                     SVGEVYAPFDGEIISLFPTKHAIALKDDKGVEVLIHIGIDTVELNGEGFEQLVKVGDF
                     VKRGQLLLRMDIDFISSKGYSLISPVVVTNSIDQLEIIVKDAETMVTNEDDLLVIL"
     gene            10627..12054
                     /locus_tag="VBC52_000963"
     CDS             10627..12054
                     /locus_tag="VBC52_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEO6882560.1"
                     /translation="MTVFPKHFLWGGAVAANQVEGAFRTDGKGLSVQDVLPNGGLGDF
                     TAKPTPDNLKLEAIDFYHNYKNDIKLFAEMGFKVFRTSIAWSRIFPNGDDSAPNEAGL
                     QFYDNLFDELLKYNIEPLVTLSHYETPLHLAKTYNGWADRRLIAFFEKFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGAIMTDKSQLSPQELYQAIHHELVASARVTKLGRSI
                     NPNFKIGCMILAMPAYPMTSDPRDVLAARQFEQHNLLFSDIHVRGKYPTYIQSYFKNN
                     GIKIKFEEGDEEVLAQNTVDFLSFSYYMSVTQAYDFENYQSGQGNILGGLTNPHLTTS
                     EWGWQIDPIGLRLVLNQYYERYQIPLFIVENGLGAKDQLIETLDGDYTVEDDYRIDYM
                     NQHLVQVAKAIEDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGTGSLQRY
                     KKKSFGWYQKVIKTNGQSLFEHHNR"
     gene            complement(12123..13217)
                     /locus_tag="VBC52_000964"
     CDS             complement(12123..13217)
                     /locus_tag="VBC52_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6882561.1"
                     /translation="MKLRKQLAQQIVTSIKDVCQQDINFINTKGIIFASTNPKRVGEF
                     HEIGLKVAQTGQMIEVTDQESYFGTQAGINIPFYYNCELLATIGISGNPNQVGKYALL
                     AQKMTRLILKEHELDYLDFGRKNEASIVLHHLVEGRELDYYYLNQFLNQYHLSEKTDY
                     RLLTFEINSQKQKLLLSQSEMSLLNFFDKLDTAIYTFNYPNQYWLLLSDQMFDYYYPN
                     ILSKFECEKGLYKVGIGQKSSLSLLKRSYETSILALKALKGQQKVNLVDDLDLELLLT
                     SIDSNIKQYVLNKALVNLSENDKILLNSYFKHNLSLKECSQELFIHKNTVQYRLNKIY
                     ESTQLNPRNFKDATLLYLALKSEDINLHKL"
     gene            13384..14526
                     /locus_tag="VBC52_000965"
     CDS             13384..14526
                     /locus_tag="VBC52_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEO6882562.1"
                     /translation="MKVVVAIDSLKGSLSSLEAGNAIKESINEVISGADVEVHPLADG
                     GEGTVEALTLGMGGTIETIPVKGPLGEKVHASYGIIPQRQLAIIEMAAAAGITLIATE
                     ERNPLHTTTYGVGEMIKDAISKGCRHFIIGIGGSATNDGGAGMLQALGYALLDKDNQE
                     ISLGAQGLADLKSISTDKVIEELKECDFKIACDVTNPLCGAQGCSSIFGPQKGADEDM
                     ITKMDTWLSNYATLATSVSEKADATIEGTGAAGGLGFAFLAFTNATLEPGIDIILSEI
                     NIEKAISEADLVVTGEGRLDGQTVMGKAPIGVAKLAKKYGKKVVAFSGSVTEDAILCN
                     QHGIDAFFPIVRRLISLDEAMSKEVAYKNMKETATQVFRLINLYNH"
     gene            14551..15807
                     /locus_tag="VBC52_000966"
     CDS             14551..15807
                     /locus_tag="VBC52_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEO6882563.1"
                     /translation="MEATFTTTGALIGLALAILLIIKKVHPAYSLILGALVGGLIGGG
                     DLVTIVNTMVLGAQGMMSSILRILTSGILAGALIKTGSAEKIAESIIKKLGQQRAITA
                     LAIATMIICAVGVFIDIAVITVAPIALAIGKKANLSKSSILLAMIGGGKAGNIISPNP
                     NTIAASEAFKVDLTSLMVQNIIPAIAALVVTIILAKIVSKKNNDISYDSEEQVGSDLP
                     AFLPAISGPLVVICLLALRPLFGITIDPLIALPLGGLISILATGYLKETVPFVEYGLS
                     KVVGVSILLIGTGTLSGIIKASNLQFDMIHLLEFLNMPTFILAPLSGIFMGAATASTT
                     SGTTIASQTFAETLIKSGVPAVSGAAMIHAGATVLDSLPHGSFFHATGGAVNMAIKDR
                     MKLISYEALIGLTSTIVAVVYYCFFG"
     gene            15908..16972
                     /locus_tag="VBC52_000967"
     CDS             15908..16972
                     /locus_tag="VBC52_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6882564.1"
                     /translation="MKQTKKEFILGLTIIITLTLLIMLIFLVMFNKSSNSKLKQTKDR
                     RISSTSTTRTSSSSHDQKIVEKSLEKKKMVMEQDMELMNAYRLYYDYAHLSLKEVVLA
                     YMSEYGIEKNSVAFSYKNLKTGQRDAMNDTQAMTAGSTYKLPLNMLVVDAVVKGKLSM
                     EERFDITETDYEYIGEHNNYVAAFSGAMSIDDMQKYSLVYSENTPAYALAERIGGMDS
                     AYSKFGRYGQSKGDIKNIQKNGNKVTTDYYIQVLDYLWKHRKKYDSLITYLEEAFPTD
                     YYRALIPSDVVVAQKPGYVREALNVGAIVKEEVPYIVAIYTAGLGGSTQEDSEINGVG
                     LYQLEQLCFVINQWHRVNMN"
     gene            complement(17027..17362)
                     /locus_tag="VBC52_000968"
     CDS             complement(17027..17362)
                     /locus_tag="VBC52_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6882565.1"
                     /translation="MTYLQYVILLVLWEEDHRHMKELSYALSLNSNTLTPVLKRMENR
                     GWIVREKPRDDKRQLIIHLSNKGIESQDAVVNAVQSCGYFFSEISPESYQKTVSALSS
                     LNQVIKSHL"
     gene            complement(17551..18579)
                     /gene="queA"
                     /locus_tag="VBC52_000969"
     CDS             complement(17551..18579)
                     /gene="queA"
                     /locus_tag="VBC52_000969"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEO6882566.1"
                     /translation="MNTNDFDFYLPEELIAQTPLEKRDASKLLVIDHKNKTMTDSHFD
                     HILDELKPGDALVMNNTRVLPARLYGEKQDTHGHVELLLLKNTEGDQWEVLAKPAKRL
                     RVGTKVSFGDGRLIATVTKELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     RDRYQTVYAKENGSAAAPTAGLHFTKELLEKIETKGVKLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSKEAADTLNAVKESGGRIVAVGTTSIRTLETIGSKFNGELKADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLEAYNHAVEERYRFFS
                     FGDAMFVK"
     gene            18732..19412
                     /locus_tag="VBC52_000970"
     CDS             18732..19412
                     /locus_tag="VBC52_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEO6882567.1"
                     /translation="MKEAKNFSQQLNILRAGVLGANDGIISVAGVVIGVASATHNLWI
                     IFLSAASAILAGAFSMAGGEYVSVSTQKDTEQAAVAREEKLLENNPELAKKSLVDIYL
                     AKGESHEHAQWLVDKAFSKNAIEHLVEEKYGIEFGEYTSPWHAAISSFIAFAIGSIFP
                     TITILLLPFSVRIVGTVIIVIVSLLSTGYVSAKLGQAPTVPAMRRNVMIGCLTMLATY
                     VIGQLFSI"
     gene            19563..20264
                     /locus_tag="VBC52_000971"
     CDS             19563..20264
                     /locus_tag="VBC52_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEO6882568.1"
                     /translation="MRVITVKNDIEGGKIAFTLLEEKMKAGAQTLGLATGSSPITFYE
                     EIVKSNLDFSNMVSINLDEYVGIAASNDQSYSYFMHKHLFDAKPFKENNLPNGLAKDL
                     KEEIKRYDAVINANPIDFQILGIGRNGHIGFNEPGTPFDITTHVVDLAPSTIEANSRF
                     FNSIDDVPKQALSMGIGSIMKSKTIVLVAYGIEKAEAIASMIKGPITEDMPASILQKH
                     DDVVIIVDEAAASKL"
     gene            complement(20336..21292)
                     /locus_tag="VBC52_000972"
     CDS             complement(20336..21292)
                     /locus_tag="VBC52_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO6882569.1"
                     /translation="MGLLVDGKWVDQWYDTASTGGKFVRTVTQFRHWVTKDGSAGPSG
                     DAGFKAESGRYHLYVSLACPWASRVLIMRKLKNLESHISISIVNPLMLENGWTFQEYK
                     GVIPDMINQSQYLYQIYQASQSDYTGRVTVPVLWDKKFHTIVNNESSEIMRMLNTAFN
                     HITGNTDDYYPDSLQGQIDEMNNFIYPKINNGVYKAGFATSQNVYQKEVETLFTALDQ
                     LEKHLSDNHYLVGEQFTEADIRLFTTLVRFDTVYYGHFKCNLKALHDYPHLWHYTKRI
                     YNLPGIAETVNFDHIKKHYYGSHKTINPTGIIPAGPNLDWTI"
     gene            21388..22107
                     /locus_tag="VBC52_000973"
     CDS             21388..22107
                     /locus_tag="VBC52_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6882570.1"
                     /translation="MRLDKLLGQAGFGSRNQVKKLICSRQVSVDGQIVTKDNVIVDSG
                     LQSIFVGKERVCLKESSYYLLYKPSGVVSAVRDSEHKTVIDLISEKDKVEGLYPIGRL
                     DRDTEGLLIITNNGPLGYRMLHPKHHVAKTYYVEVNGFLERDAITFFEEGVVFDDGTK
                     CKPAELTIDTANNDKSTARITITEGKFHQVKKMFLAYGVKVIYLRRISFGDLRLDMNL
                     KPGQYRRLRDSEAAILKRYLD"
     gene            22180..23331
                     /locus_tag="VBC52_000974"
     CDS             22180..23331
                     /locus_tag="VBC52_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6882571.1"
                     /translation="MNSKLSLKLTILSISIFLMSHLAIAPAIPKLYAFYHTQNPHIGL
                     ASVESLVTIPAMMITIFVILSNFVVTKLGKKNTVLLGLCLILMSGFISFFTSNFSLAM
                     ASRLLLGIGIGLYNSLSISIITDLYEADERASMIGLRTASLNIGKALTTFIVGLVLAI
                     GVNYIYLVYLLVIPVFFFFWKNVPEVENQTHTLKASTTFDTKAALLMLITFLVGIAYI
                     GATVKIPTLLVTKYHYATSFSSNMLTLLAFSGILVGSVFGKLVKVFQEKTLLIMILAM
                     GIGNVLFALANNQIIFIVASILIGASFVGTMSSVFFYISKNYAKEHNNFITSLALTAG
                     NIGVILTPLILTKLPSQLHLEPFMTPFLITSGLMVINVFVYLVLMSKNK"
     gene            23383..24330
                     /locus_tag="VBC52_000975"
     CDS             23383..24330
                     /locus_tag="VBC52_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEO6882572.1"
                     /translation="MLIAKDKQGNLINLLESHPGKGQYFCPTCCSAVRLKAGRIMRRH
                     FAHISLKNCQFYHENESNEHLQLKAKLYMSLSRENETMLEHHLPEINQIADLFVNETL
                     ALEVQCSRLSEQRLRERTKAYLQADFQVRWLLGEKLWLKHRLTNLHKQFLQFSQSIGF
                     HIWELDLRLEVLRLKYLIYEDLRGHVYYLSKTCPLSGDVLAFLKWPYQSKNLNFYKVK
                     QDRNIRDYVRQQLRYGNQFWLRKQEKAYLSGQNLLTQELMMFFPQIQPPRVDTDFCQI
                     TNSLTSFYQNFTNYYQKNKNNLDQTLYPPVFYDKIVTKN"
     gene            24346..26151
                     /gene="pepF"
                     /locus_tag="VBC52_000976"
     CDS             24346..26151
                     /gene="pepF"
                     /locus_tag="VBC52_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO6882573.1"
                     /translation="MSDNRSHIEEKYQWDLTTVFATDELWETEVVELTQAIDNAKGFS
                     GHLLDSSQSLLEITEVELDLSRRLEKVYVYASMKNDQDTTVAKYQEFQAKATALYAKF
                     SETFSFYEPELLQLSESDYQSFLLEMPDLQKYDHFFEKIFANKPHVLSQNEEELLAGA
                     SEIFGAAGETFEILDNADMVFPVVKNAKGEEVELTHGNFISLMESSDRTVRKEAYQAM
                     YSTYEQFQHTYAKTLQTNVKSQNFKARVHHYQSARQSALSANFIPEEVYETLIKTVNH
                     HLPLLHRYMKLRQKVLGLDDLKMYDVYTPLSQMDMSFTYDEALKKSEEVLAIFGEAYS
                     ERVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRENQPYVYGDYSIFLAEIASTTNENILTETLLKEVKDDKNRFAILNHYLDGFK
                     GTIFRQTQFAEFEHAIHVADQEGQVLTSEYLNNLYAELNEKYYGLTKEDNHFIQYEWA
                     RIPHFYYNYYVFQYATGFAAANYLAERIVNGNPEDKEAYLNYLKAGNSDYPLNVIAKA
                     GVDMTSADYLDAAFRVFEERLVELENLVAKGVHND"
     gene            26346..>26790
                     /locus_tag="VBC52_000977"
     CDS             26346..>26790
                     /locus_tag="VBC52_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEO6882574.1"
                     /translation="MKKLTFIWDLDGTLIDSYVPIMEALEETYRHFGLIFDKELIHEY
                     ILQESVGKLLVNLSEEEQIPHEKLKAYFTKEQESRDSKIHLMPYAKEILEWTKEQDIP
                     NFMYTHKGASTHSVLETLQISHYFDEILTGVSGFERKPHPQGINYL"
BASE COUNT         8727 a         4072 c         5142 g         8849 t
ORIGIN      
        1 tttatcattc caggcagatg cttcaggtaa cacttatcaa gctttttaca cattaaaaaa
       61 taaaagtgag aaagattatt ttcaaaatct tgataataat ataatgataa ttgcagatat
      121 caaacttgaa gaagcagagg agagaaggca ttttaatggc tttgattatc gtcagtattt
      181 aaaaagacat ggaatttatc gtatcgccaa agtgacaaag ataaaacaga tacgcttatt
      241 tcaacatagg tctttctttg ctcttatgtc taagtggcgt agaagtgcaa ttgttattag
      301 tcaaactttt ccaaatccta tgcgtcacta tatgtcaggg cttttgtttg gatatctaga
      361 taagaccttt gatgacatgt ccgatttata tagtagtcta ggtattatac atttatttgc
      421 tttgtcaggt atgcaagtag gtttttttct cggtattttt cgttatatct gtctacgtat
      481 tggcttacgt ctagaccatg tttggttact tcaaatacca ttctcgctaa tttatgctgg
      541 tttaacaggc tttagtatct cagtcgttag ggcacttatt caatctttat tatcacatag
      601 cggtgtcaag aaagatgaga actttgctct ctgcttgtta atttgtctta tctccctccc
      661 ccactcactt ttgactacgg gaggagttct tagctttgct tatgctttta tacttacgat
      721 gacctccttt gatcattttt cgagtataaa aaaagtagct atcgaatctt tgacagtctc
      781 tgtaggaatt cttcccatac taacctacta tttttcgggt tttcaaccaa tatcaattat
      841 attaacagca cttttatctt ttgcatttga tattatattt ttgcctttat taactgttat
      901 atttgtctta tcgcctatcg ttaaattaag ttgtattaat agtttgtttg aaatcctaga
      961 agtgttatta aaatggactg ggcaactgtt tccaaggcca cttatttttg gaaagcccag
     1021 cctttttctt ttaatagtca tgattataat tttgggatta ctttatgatt attatcattc
     1081 taaatgtttt cgttattgct cccttcttat tatctttacc ttgtttttta tcactaagaa
     1141 tccaattact aacgaggttg cgattttaga tgttggacag ggagatagta ttttagtgag
     1201 ggattggtta ggaaaaacaa ttttaattga tactggggga agggtgagat ttgaacagcc
     1261 tgaagaatgg aaacaaaaag taaatcagtc taatgctaag agaacgctca ttccttactt
     1321 gaaaagcaga ggtattagca agatagatga tttagtgata actcataccg atacagatca
     1381 tatgggggat atggaagtta tctcaaagca ttttaaagtt gcacgtttga ttacaagttc
     1441 aggttcttta acgaattcgc agtacgttaa gcatttatca aagataggtg tagcggtaaa
     1501 atctatagaa gccggtgata aacttgctgt catgggaagt tatttacaag tactttaccc
     1561 atggcacaag ggtgatggaa aaaataatga ttcaattgtt ttatatggac atttattagg
     1621 aaaaggcttc ttatttaccg gtgatttgga ggaagaggga gaaaagcagt tattagaagc
     1681 ttatcctaat ttatcagtag atatccttaa agcaggacat catggttcta agggctcatc
     1741 aagtctatcc tttctgaaaa agttgtctcc tagtgtggtt ctagtttcag ctggtaaaaa
     1801 taatcgttac cagcatcctc atcaagagac tttacaaagg ttccaaaaga ttaaaagcaa
     1861 gattttccga acggatcaat caggtacaat taggctaaca ggatggtgga agtggcatat
     1921 tcagacagtt cgttgattta taaaaatgat tgcgtatctg ttaaatacaa tctatttttc
     1981 gcaagaattc agtaatttta agcttgacat ggaacgggtt ccagcattta tactgatttc
     2041 agaagaagag aggtattttt tatgactatc aaacgtattt tttgtgacat ggatggtaca
     2101 ctattaaata gtgaaggaca agtgagtaaa agtaacgcta ccttaatacg tgaggcagcg
     2161 attccagtaa ctttagtgtc tgctagggca cctatggaaa tgaaggatgc cgtagatgca
     2221 cttcaattag gaggagtcca agttgctttt aatggaggat tgatttatag aataggagat
     2281 aataatcaag ttctacctat tcatacacaa atcattaaaa aaagcacagt aaaacaattg
     2341 ctaagaggaa tacggtttca ttttcctcaa gttagtttat cttattacga tcttaataac
     2401 tggtattgtg ataaaataga tgaaggaatc cgatacgaac atagtttaac tcaacaatgc
     2461 ccaacgttta ttcataatga ggatcagttt ttagaaggtc acacaaatac ttttaaaatt
     2521 atgatgatta ctttcgatga agcaaatatg ctagaattag aaaaatatct tcaaagtttg
     2581 gagttaccag aaatcactat tcaacgttca ggtaaagcgt atcttgaaat tacacatctc
     2641 ttagccaaaa aatctaaagg aattgcttac atccttcaaa aagagcagtt agctagagaa
     2701 gaaacggctg catttggaga tggacataat gatttgccta tgcttgaaat ggtaggttac
     2761 cctattgtta tggataatgc ttttgatgat atcaaagcga tagcatatca actaactaag
     2821 tctaatgatg aagacggagt gggatatggg attcaaaaat ttttaaagta ggagccttat
     2881 aatgacaagt attagtgata tagctaaaaa ggcaggtgtt gctaaatcaa cagtttcccg
     2941 tgttatcaat catcacccac atgtttctga tgagacgcga caaaaagtaa tggctcttat
     3001 caccgaattg gattatatac caaatcaact agcgcgggat ttaagtcgag gcaaaacaca
     3061 aaaaataggt gtcgtcattc cccacacgcg ccatccttat tttacacaat taataaatgg
     3121 cctattagat gctgctaaaa ctaccgacta tcaacttgtt atgatgcctt cggattacaa
     3181 ccaagaatta gaactttcct acttgaaaca gctcaagatg gaagctattg atgctttaat
     3241 attcacttca agagcaatta gcctcgatat tattgaaact tatgcaaaat atggacgaat
     3301 agtagtttgt gaaaaattgc aggaatataa tcatctatcc tctgcttacc ttgatcgcta
     3361 ttcttcattt ctagaggcgt tttctgatat gaagttacga ggtttggagc acttagtttt
     3421 attattttca aggaataatg aatcctctgc tacctatcaa tccgcattat tagcttatca
     3481 agaggtctat gggcaactat cctctccata tatggtagtt gggaatgttc atgactttaa
     3541 tgatggacta aatttatctt atcaattggt aaaagaagtt agtatagatg gtatattagc
     3601 tacaagtgat gaagttgcgg cagggctaat aaaaggttat gaagaaagta ggaaaaaatg
     3661 tccttatatt attggtcagg aatgtttatt agttgggcaa cttttgaaac tcccaacaat
     3721 tgaccacaaa tcctattatt tagggaaatt agcttttaaa caggctttag ctgaaaagat
     3781 tagtcaagaa gttttattct cagagttttt atctcaagac aattagtcat tcaaaagtaa
     3841 tagaaaaaag gtttccaact aaacttgatt ggaaaccttt ttatttaatg attgataatt
     3901 ttaagcattt gtttgaaagc agtctctgct tcaggaatcg gtaagactgg ataaacgtgg
     3961 ttcatttttt gaccaacgat atagtggtgt ttaatgtttt cttttgctaa tttttgatgt
     4021 aataattgat tatcggggta gaaaatatcg tgcgttccta taaaaaatga tattggagcc
     4081 atcttgttaa attggctact gtaaagagga ctaacttgag gatttttaaa attctttttt
     4141 ccatttgccc agtaaggagc tacttgttgt aattgccaag ctttaagaag gggatctttt
     4201 ttttgaactt tttcaatctt tggattatta gtagcaatat ctaaccatgg tgatattaaa
     4261 ataatttctt ttggttgttt gatatgctcc gtgacaaggt tatcagcaag acctaaagca
     4321 agacctccgc ctgcactttc acctactagg ataatttgtt tgggagatgt gacgctagct
     4381 aatgtatttt ggtataattt tttaattttg gggatagcat cactataatt atatgtagga
     4441 gctttagggt aaataggaaa gacaactttt gcatctaatt ttttagctag tttattgaca
     4501 aaaatatatt gtaattcaga agcttgatgg atatatgatc ctccatgaac atagaagata
     4561 accttttgct taggattatt tttatcattc caagtgaatg tttgcatgcc atcagtagcg
     4621 gtaatttcta ccttttcatt atttaatgcc tgaggaatgt ctacaggaag aacattacgt
     4681 ttagctacct tagttaagta attttggtat tctttgatgg tttgaggctt aatctgattt
     4741 accacaggta gagttttttc aatagtattg ctagttaggg atcttttttc aacaatatgc
     4801 gtgtaagcat atgctcccat aaaaatgaca aaaaaggtta gaagtagtag tacaataagt
     4861 tttcgatagt ttttcatgat tatccttttg taattgagct cgtcatgcta aaaatgatta
     4921 gttgttattc tataataaag tagatacgaa aacaagaaaa taatgctata aaatttcaac
     4981 ctattataat tttttgctga tagtacgtat taacctttaa tttgctaaaa tagaagagag
     5041 gagagtgtta ttatgtttca cagagaagat ttttcaaaag cattattaaa aaaagctttg
     5101 cagcccggta tgagagttat ggatattggt tgtggtagtg gggaattgac gcgtttggct
     5161 gctgatatcg ttggtaaaga aggtgatgtt gtaggaattg atattaatga gcaactttta
     5221 tggcaagcta gacatgataa tggctatgct aatgttagtt atcaaaacgc agatttagca
     5281 gcactttcct cctttgaaac aaaaagtttt gatgcaatta ttggacgtcg tgtattaatg
     5341 tatcaaaaag atccaaagaa agtaatagaa agtttaacac ctttgttaaa gccaaatggt
     5401 ttttatgttt ttcaagaaag tgatgcaatg tgtttagaac tagctaagcc tagctttgta
     5461 aatatgaata aaatacaatc actaatctgg caaactgttg agaaggaagg aggacacatt
     5521 catattggaa gtcaacttga gcaattaatg ttagaagcag gcttaatcgt tgaccatttt
     5581 gtatcagaaa atgtcattca aacgtcacaa actggtacag atttatcttg gctactaaat
     5641 atcatgaaag aaagaataca aaaatatagt gaccttcagg gattagaaga tgtcaattta
     5701 caaaaagaat tagaagagaa taatgaccat tttatccgag attgtcaatt tggaattatt
     5761 tctcatagag ttgatgaatg attttaagta ttatttgaca ccttgaaaag tttccttatg
     5821 gtataataag gaatatgatt gcgattgaag aaattgggag aattacccca gataatttag
     5881 ggttagtaac tgttttagca ggagaagatt tagggcaata tgctcagatg aaagagaagc
     5941 tatttcaggt aattggattc aataaagacg atttagccta ttcttatttt gatttaagtg
     6001 aagaagacta tcaaaatgcg gaattagact tagaaagttt acctttttta tcagactaca
     6061 aggttgttat ttttgatcag tttcaagata ttacaactga taaaaaaact tatttagatg
     6121 aacaagcaat gaagcgcttt gaagcctact tgcagaatcc tgtagatact acgagacttg
     6181 ttatttgtgc tccaggaaaa cttgatggaa aacgtcgact tgtaaagtta ttgaaacgtg
     6241 atgctcgtgt tttagaggct aatactttaa aagaatcaga tttaaagacg tattttcaaa
     6301 aatatgctca ccaagaagga ttagtttttg aagcaggtgt ttttgatgag ttattgatta
     6361 agtctaacta tgattttagt gatactttaa cgaatatcgc tttcttaaag tcatataaaa
     6421 cagacggtca tatttcgtcc aatgatgtta gagaggctat ccctaaaagc ctgcaagata
     6481 atatatttga tttgactcaa gatgtcttac ttggcaggat agatttagcg cgtgatttag
     6541 taagagattt acgtctacaa ggcgaagacg aaatcaaatt gattgcaatc atgttaggac
     6601 agtttcggat gtttttgcaa gttaaaatat tggctagtaa agggaagtca gagagtcaaa
     6661 ttgtttctga actatcccat tacataggac gtaaaatcaa cccttatcaa gtaaaatttg
     6721 ctgtcagaga ttctagaaac cttccccttg cttttttaaa agaagcgatt aggattttaa
     6781 tcgagactga ctatgctatt aaaagaggga cttatgataa ggattatctc tttgatttag
     6841 ccttattaaa aattgctcat aaaaagccct aattaaagaa acaaaagagt tgaaagctgt
     6901 tccaaattcc tatagaataa gggtaataat agatgaaaag aggatttcga catggcaatt
     6961 attttaccag atttacccta tgcatatgat gcgcttgagc cacatattga tgctgagaca
     7021 atgacactac atcatgataa gcaccatgca acttatgttg ctaatgcaaa tgctgctctt
     7081 gagaaacatc ctgaaattgg agaagactta gaggcgctct tagctgatgt ttctcaaatt
     7141 ccagaagata ttcgtcaggc agtcatcaat aacggtggtg gacatcttaa ccacgctctt
     7201 ttctgggaat tgatgtcacc agaagaaact caaatttcac aagagttatc tgaagacatt
     7261 aatgcaactt ttggttcatt tgaagacttt aaagctgctt tcacagcagc agcaacagga
     7321 cgttttggtt caggttgggc ttggcttgtt gttaatgctg aaggcaaact tgaagtgctt
     7381 tcaactgcca atcaagatac tccaattatg gaaggcaaga aacctatttt agggcttgat
     7441 gtatgggagc atgcttatta cttaaactat cgtaatgttc gtcctaacta catcaaagct
     7501 ttctttgaaa ttattaactg gaataaagta aatgaacttt accaagctgc taaagcttaa
     7561 taaaaaacga gtcgataggc tcgttttttt attattctaa aagttgataa tgttttttaa
     7621 aagtctagaa gtccaaactc agataaaact agaatagtcc tattatcttc aactaagcga
     7681 ataagtttat tgaaagcgct tgacaagatt agtaagaaat gataaaatta agacgttttc
     7741 aggtgttatt atttaaggta agcaagctga taacagataa aattgcataa taaaggatgt
     7801 tatcacattt tgtgagcagg gacctattca acgacgactt tttgttgtgt cttggatggg
     7861 tcatttttta ttgtgcgagt gaaggactag gactttaatg attataaaac gcgtattaaa
     7921 tcataatgca gtcatatcag taacacatca aggattagat gtgctactga tggggaaagg
     7981 aattgcattt aagaaaagaa taggtgatag gattaatagc gatgctattg agaagtcatt
     8041 tgttttaaaa aatagtgata acatgaaccg ttttacggag ttatttatta ctgttccaga
     8101 agaagtagtt gcatgttcag aacgaattat caacctaggt aaaatcaaat tgggtaagaa
     8161 cttagatgaa attctttata tcaatttgac agatcatatt cattcggcta ttgaaagaca
     8221 tgaacaagga atggttatcc aaaatccact aaggttggaa atccaacgtt actatcctga
     8281 tgaatactcc attgggatga aggctctgga acttataaag gatgaattgg gcatttgttt
     8341 aacaatcgat gagtcggctt ttattgctat gcactttgta aatgctggat tggataatcc
     8401 gtttaatgaa gcgcataaaa tcactgagat tgtatcttac attgaacaga aagtaaaaat
     8461 agattttcga acagaactag atgaaagttc aattgattat tatcgtttca tgacacatac
     8521 caaattattt gctcaacgcg ttttatcagg aatgaaatac gaagatgatg atgctgattt
     8581 acttttggta gttaagaaaa aatatccgag agaatataaa tgtgttaaag agatcggcaa
     8641 caatatggct atccaatacc aatatcaact taattcaagt gaactcctat atctgacggt
     8701 gcatgttaaa cgattagtta aaaatttaaa ggagatatca gatgactaaa taccaagaga
     8761 ctgctaaagc tattttagca gcagtagggg gagaaaaaaa tattcaacac gtaacccact
     8821 gtgtgacacg attacgatta gttttagaca atgatgaaat tgtcaacgat caggttatta
     8881 aaactattcc gaatgttatc ggtgttatgc gtaaaaatga tcaataccaa attattttgg
     8941 gaaatgatgt caataattat tataatgctt ttttggcctt aggtcatttt gaaaatacta
     9001 ccagagaatt ttcatcacaa aaaaagagtt caatacttga gaaattaatt gaaacgattg
     9061 caggagtcat tacgccgctt atacctgctc ttctaggagg agggatgctc aaggttatag
     9121 gtattttgct ccctatgctc ggtatagcaa gctctagttc tcaaacagta gcttttatca
     9181 atttttttgg tgatgccgct tattatttta tgccaataat gattgcttat tcagctgcgt
     9241 cacgatttaa agttacacct gtattagctg ctacagtagg agggattctc ttacaccctg
     9301 cttttgtgac aatggtagct gaaggaaaac cattatcttt atttggagct ccggttacac
     9361 tggctagtta tggttcttct gttattccaa tcttaattat ggtttttctt atgcaatata
     9421 ttgagagatg gattaataaa attgttccta gtgtaatgaa gagtttttta caaccgactt
     9481 tgattattct catttctggc tttttagctt tagtggtcgt ggggccactg ggggtaatta
     9541 ttggtaaagg attgtctagt gcaatgcttt cgatttacca tgtggcgcca tggttagctt
     9601 tatctattct tggtgctatt atgccattgg ttgttatgac aggaatgcac tgggcatttg
     9661 ctccaatatt tttagctgct tctgttgcga caccagatgt tttgatttta ccagcaatgt
     9721 tagcttctaa tttagctcaa ggtgcggctt ctcttgctgt tgcagttaag gctaaacaaa
     9781 aacaaacacg tcaggtggcc tttgcagcag gtttatcagc actactagct ggtatcacag
     9841 aacctgcact ttatggtgtc acactgaaat tcaagaagcc tttatacgct gcaatgatat
     9901 caggtggttt agtaggagct tatattggat tagttaatat tgcttcctat acatttgtag
     9961 taccatctat tattggtttg ccacaatata tcaatccaca ggggggcaat aatttcagta
    10021 atgctgttat tgcagcaata gctactatta ttttgacttt cattattaca tggttcttgg
    10081 gaattgatga aggagaaaac gaaaaaagta gtattaatgc tcaagagcac acacatatta
    10141 gaagtggttt atcaaaaaaa gagacattgt attcacctat ggtaggcaat gtattacctt
    10201 tatctaaagt acccgatgaa acattttcat ctaaattact aggtgaaggt ttagcaatca
    10261 ctcctagtgt gggtgaagtt tatgcaccat ttgatggtga aattataagt ttatttccta
    10321 caaaacatgc cattgcctta aaggatgata agggagtcga agtgttaatc catattggaa
    10381 tagatactgt agaactaaat ggagaaggtt ttgaacaatt ggtcaaagtt ggcgattttg
    10441 ttaaacgagg acagttactt ttgcgaatgg atattgattt tattagttca aaggggtatt
    10501 ctcttattag tccggttgta gttacaaatt caattgacca acttgaaatt atcgttaaag
    10561 acgcagagac aatggttaca aatgaagatg atttacttgt aattctttaa aaggaaggat
    10621 gataatatga cggtttttcc taaacatttt ctatggggag gtgcagttgc tgccaatcag
    10681 gtagagggtg catttagaac agatggcaaa ggtttatctg tacaagatgt acttccaaat
    10741 ggtggtttag gcgatttcac cgccaaacct actccagaca atttgaagct agaagcaatt
    10801 gatttttatc ataactataa aaatgatata aaactgtttg cagagatggg ctttaaagtt
    10861 tttagaacct ctattgcctg gtctcgaatt tttccaaatg gtgatgatag tgctccaaac
    10921 gaagctggct tacaatttta tgataattta tttgatgagt tgcttaaata taatatcgag
    10981 cccttggtta ccttatctca ttatgaaaca ccgcttcatc ttgcaaaaac ttataatggt
    11041 tgggctgata gacgtttgat agcatttttt gagaaatttg ctcaaacagt catggagcgt
    11101 tataaagata aggtgaaata ttggttgact tttaatgaag tgaattctat tttacatatg
    11161 cctttcacca gtggtgctat tatgaccgac aaatcacaac taagtcctca ggagctttat
    11221 caagctattc atcatgaatt agtagcatcg gctagagtga caaaattagg gcgttctatc
    11281 aatcctaatt ttaaaattgg ctgtatgatt ctggctatgc cagcttatcc tatgacatct
    11341 gatccaagag atgtcctggc tgcaagacaa tttgaacaac ataatctgct attttcagat
    11401 atacatgtca gaggtaaata tcccacttat attcagtcct atttcaaaaa taatggtatt
    11461 aaaattaaat ttgaagaagg agatgaggaa gtattggcac aaaatacagt agattttcta
    11521 tcgtttagtt actatatgag cgtgacacaa gcttatgact ttgaaaatta tcaaagtggt
    11581 cagggaaata tccttggagg cctaactaat cctcatttaa caacttcaga atggggatgg
    11641 caaatcgatc ccatcgggct acgcctagtc ttaaatcagt attatgaacg ttatcagatt
    11701 ccgttgttta ttgtagaaaa tggattggga gcaaaagatc aattgataga aacactagac
    11761 ggtgattata ctgtcgaaga tgattatcgt attgattata tgaatcaaca tttagttcaa
    11821 gttgctaaag ctattgaaga tggtgttgaa ataatgggat atacatcttg ggggtgtatt
    11881 gattgtgtat ccatgtctac agctcaacta agtaaacgtt atggtctaat atatgttgat
    11941 cgaaatgatg atggaacagg gagtctacaa cgttacaaga agaagtcatt tggttggtac
    12001 caaaaagtga ttaagacaaa tggccaatca ttgtttgaac atcataacag ataacatgtt
    12061 aaacaactag tctctgtttc aatttaatat aaaaaggagt atccataggt tactcctttt
    12121 tattataatt tatgaaggtt aatatcttct gatttaagtg ctagatacaa taaagttgca
    12181 tccttaaagt ttcttggatt gagttgcgtt gattcataaa tcttatttaa cctatattga
    12241 acagtatttt tatggataaa aagttcctga ctacattctt ttaatgatag attatgctta
    12301 aaatatgagt taagcaagat cttatcattt tcagataagt ttacaagcgc tttatttagc
    12361 acatattgtt taatattaga atcaatactt gttaataaca gttcaagatc aaggtcgtcg
    12421 acgaggttga ccttttgttg accttttaaa gctttcaggg ctaagataga cgtttcatat
    12481 gatcttttta aaagtgataa agatgatttt tgtccgatac caactttata gaggcccttt
    12541 tcgcattcaa actttgatag gatatttgga taataatagt caaacatctg gtcagaaagt
    12601 aaaagccagt attgattggg ataattaaag gtataaatag cagtgtcaag tttatcaaaa
    12661 aagttcaaga gtgacatttc agattgactt aagagtaatt tttgtttttg actattgatt
    12721 tcaaaagtga gtagacgata gtcagttttt tctgataggt ggtattggtt taaaaattga
    12781 ttgaggtaat agtagtctaa ctctctgcct tcaacaagat gatgtaagac aatagaagct
    12841 tcgttttttc gcccgaaatc cagataatct aattcatgct cttttaagat gagtcgagtc
    12901 attttttgag caagtaaagc atattttcct acttggttgg ggtttccact aatcccaata
    12961 gtagctaata attcacaatt ataatagaaa ggaatattga tccctgcttg tgtcccaaaa
    13021 taagattctt gatctgttac ttcaatcatt tgtcctgtct gtgctacttt taaaccaatt
    13081 tcatggaatt ctccaacacg ttttggattg gtactagcga aaatgattcc tttggtatta
    13141 ataaaattaa tatcttgttg acaaacatct ttgatagagg taacgatttg ttgtgcgagt
    13201 tgttttctta atttcattca gcttaccttt tgttatatat aacaaatatc atatcataaa
    13261 aacgctaaca tttgttctgt agtctatatt ttttgttatt tgattttgtt actataattg
    13321 agtgatataa aatagtgaaa aaaataacaa agattcacaa aatattttca aggaggtttt
    13381 caaatgaagg tagtagtagc tattgattct ttgaaaggga gtctttcttc tttagaagct
    13441 ggcaatgcta taaaggagtc aattaatgaa gtaatctcag gggcggatgt ggaagtacat
    13501 ccattagcag acgggggaga aggaactgtt gaagctttga cgctaggtat ggggggtacg
    13561 atcgaaacaa tccctgttaa ggggcctctt ggagaaaaag tacatgcgag ttatggtatt
    13621 attccacagc gtcagttagc tattattgaa atggctgcag ctgctggtat taccctaatt
    13681 gctactgaag agagaaatcc tctccacaca accacttatg gagttggaga aatgataaag
    13741 gatgctataa gtaaaggctg taggcacttt attattggta ttggaggtag tgctactaat
    13801 gatggtgggg caggtatgct acaagctttg ggatatgcat tattagataa agataatcaa
    13861 gaaatcagtt tgggagcaca aggattggca gatttaaaat ctatctctac tgataaggtt
    13921 attgaggaat taaaagaatg tgattttaaa attgcatgtg atgtaacaaa tcctttatgt
    13981 ggtgctcaag gttgtagttc gatttttgga ccgcaaaaag gtgctgatga agatatgatt
    14041 acaaaaatgg atacttggtt aagtaattat gctaccttag ctacttcagt tagtgaaaaa
    14101 gcagatgcaa ctattgaagg aacaggtgca gcgggtgggc ttggatttgc ctttttagcc
    14161 tttacaaatg ctaccttaga acctggtatt gatattattc tatcagaaat taatatagaa
    14221 aaagcgattt ctgaagctga tttagtagtt actggtgaag gtcgtttgga tgggcaaacg
    14281 gtgatgggaa aagctccaat tggcgtagca aaattagcta aaaaatatgg taagaaagta
    14341 gtggcatttt ctggatctgt caccgaagat gctatacttt gtaatcaaca tggtattgat
    14401 gctttttttc caatcgtacg acgccttatc agtttagatg aggctatgtc taaagaggta
    14461 gcttataaaa atatgaaaga aactgctact caagtcttta gattaatcaa tttgtataat
    14521 cattaatatt gaaaggaatt ttgataagtt atggaagcta catttacaac aacaggggct
    14581 ttaataggat tagcgcttgc tattttactg attatcaaaa aagttcatcc tgcctatagt
    14641 ctcattcttg gagcattagt tggtggtttg attggtggcg gtgatttagt tacaattgtc
    14701 aatacaatgg tattaggtgc gcaagggatg atgtcatcca tattacgcat tttaacatca
    14761 gggattttag cgggtgcttt aattaaaacc ggatcagctg aaaaaattgc agaaagtatc
    14821 attaaaaaat taggacaaca gcgagccatc acagctttag ccatcgcaac aatgattatt
    14881 tgtgcagtag gtgtctttat tgatattgca gttattacag ttgctccgat tgcattagca
    14941 ataggtaaaa aagctaatct ttctaaatct agcatactgt tagcaatgat tggtggtggt
    15001 aaggctggga atattatctc acccaatcct aatacaattg ctgcttcaga agcatttaag
    15061 gttgatttaa caagcttaat ggtacaaaat attattcctg caattgcagc tttagtagtt
    15121 acgattatat tagcaaagat tgtttccaag aaaaataacg atatttctta tgatagtgag
    15181 gagcaagtgg gctctgatct gccagcattc ttaccggcta tttctggtcc acttgtggtt
    15241 atctgcttat tagctcttcg tcctttattt ggaatcacta ttgatccctt gattgcactg
    15301 ccgttgggtg gtctaattag tattttagca acaggttacc taaaagaaac ggtaccgttt
    15361 gttgaatacg gtttatcaaa agttgttggc gtttcaattc ttttgattgg aacaggtacg
    15421 ctctcaggta ttattaaagc atctaatcta caatttgata tgattcatct ccttgaattt
    15481 ttaaatatgc cgacctttat ccttgcccca ctctcaggta tttttatggg agcagcaaca
    15541 gcatcaacga cttctggaac aactattgct tcacaaacgt ttgctgaaac cttgattaaa
    15601 tcaggggtac cggcggtttc gggtgcagct atgattcacg caggtgcgac tgttttggat
    15661 tcgctacctc atggatcctt cttccatgcg acaggcggag ctgtcaatat ggctataaaa
    15721 gatcgtatga aactaattag ctatgaagct ttgattggat taactagcac aatagttgct
    15781 gtcgtttact attgtttctt tgggtaaaat ttggatgaat acgtgatgtt tattatcact
    15841 gtttgaatag aattctcatt gattttttct aattttctta ctataataaa ttagtaggag
    15901 aggtgttgtg aaacaaacaa agaaagaatt tattttagga ttaaccatta ttattaccct
    15961 tactttatta atcatgctga tttttttagt aatgtttaat aagtccagta actctaagtt
    16021 gaagcagaca aaagatagaa gaatatcatc tacttcaacc acaaggacat ccagtagctc
    16081 acatgatcaa aaaattgtag aaaagtctct tgaaaaaaag aaaatggtca tggaacaaga
    16141 tatggaattg atgaatgcct ataggctcta ttatgattat gcccatttgt ccttgaaaga
    16201 ggttgttctg gcctatatgt ctgaatatgg cattgaaaaa aatagtgttg ctttttctta
    16261 taaaaattta aaaacaggac aaagggacgc tatgaatgat actcaggcta tgacagcagg
    16321 gagcacctat aagttacctc ttaacatgtt ggtagtagat gcagtggtca agggcaagtt
    16381 gtccatggaa gaacgctttg atattaccga gactgactat gaatatattg gtgaacataa
    16441 taattatgtt gctgctttct cgggggcaat gtctattgat gatatgcaaa aatactcttt
    16501 agtttattca gaaaatacac ccgcctatgc tttagctgag cgtattggtg gcatggatag
    16561 tgcttattca aagtttggtc gttatggcca atctaaagga gacatcaaaa atatacaaaa
    16621 aaacggtaat aaagtgacaa cagattacta tattcaagtg ctagactatc tctggaaaca
    16681 tcgtaaaaag tatgatagtt taattactta tttagaagaa gcttttccaa cagattatta
    16741 tcgtgctttg ataccatctg atgttgttgt tgctcaaaag ccagggtatg tccgtgaggc
    16801 gttgaatgtt ggagcaattg tcaaagaaga agtgccttat attgttgcta tttatactgc
    16861 aggtttaggt ggctctactc aggaagattc agaaattaat ggagtgggac tatatcagct
    16921 agaacaatta tgttttgtta ttaatcaatg gcatcgtgtt aatatgaatt agaaataaat
    16981 ctatcaaaat aataaaaacc tcttagatta actatctaag aggtttttat aaatgtgatt
    17041 ttataacttg gttaagtgaa gaaagggcag aaacagtttt ttgataagat tcaggactaa
    17101 tttctgagaa aaaatacccg cacgattgca ccgcattgac aacggcatct tgtgactcaa
    17161 tacctttgtt acttagatga ataatgagtt gccttttatc atctcttggt ttttctcgta
    17221 caatccaccc acgattttcc atacgcttta aaactggagt tagggtattc gaattaagag
    17281 atagtgcata actaagttcc ttcatatgcc gatgatcctc ttcccaaagg actaacaaga
    17341 taacgtattg gagataagtc aattgaaatt catccaaagc tttttggtaa aatttattga
    17401 aaagtcgatg tgtttcacta accgtaaaac ataatcgctc gtctaaaata cttcgattac
    17461 tcattatcat attgtagcat tttggaggcg ttatagtatg tcaatcgtgt taaaaaatga
    17521 taaaactttt tcctaattga aagcctattc ttacttaaca aacatagcat caccaaagct
    17581 gaagaaacga tatcgttctt caactgcatg gttgtaagct tctagtacaa aatcacgtcc
    17641 agcaaatgct gatactaaca tgacaagagt agactttggt aagtgaaagt tggttgaaaa
    17701 agcgtctaca accttgaact gataaccagg cttgataaaa atatttgtcc accctgagtc
    17761 ggcttttaat tcaccattga atttagaacc gattgtctct aatgtgcgaa tagaagttgt
    17821 accaacagct acaatacgtc caccagattc tttaactgca ttgagagtat cagcagcttc
    17881 tttagataat tggtaaaact cagaatgcat ttcgtgttca tcaagattat caactgaaac
    17941 aggacggaaa gttccaagtc caacgtgaag cgtcaaatag acaagcttca caccttttgt
    18001 ttcaattttt tccaatagtt cttttgtaaa atggagaccg gctgttggtg cagcagcaga
    18061 accattttct tttgcataca cagtctgata gcggtcacga tcttctagtt tttcatgaat
    18121 atatggcgga agtggcattt cgcccaaact ttcaaggact tctagaaaaa taccatcata
    18181 tgagaattct acaatacgcc caccatgttc taattccttt gtgactgttg ctattaaccg
    18241 accatcacca aatgaaactt tggtccctac tcgtaagcgt ttagcgggtt tagcaagcac
    18301 ttcccattga tcgccttctg tatttttgag aagtaataac tctacatggc cgtgtgtatc
    18361 ttgcttttca ccataaaggc gagcgggcag aacacgtgtg ttattcatta ctaaagcatc
    18421 accaggcttg agttcatcta agatatgatc gaaatggcta tctgtcatcg tcttattctt
    18481 atgatcaatg actaataatt tagaagcgtc tcttttttca agtggtgttt gggcaatgag
    18541 ttcttctggt aaataaaaat caaaatcgtt tgtattcatg atttctccta tctataagta
    18601 agcttttcta ttatatcacg ttcagtaaaa atgacccaac taatcttttt atttagaatg
    18661 attataaaaa ataattgtaa tcattctaaa taagtgatat aataattgtc agaaagtgag
    18721 aagtagagaa gatgaaagaa gcaaaaaatt tttcccaaca attgaatatt ttaagagctg
    18781 gtgttcttgg tgctaatgat ggtatcatct ctgtagctgg tgttgttatt ggtgttgcga
    18841 gtgcaactca taacctttgg attatttttt tatcagcagc ttccgcgatt ttagctggtg
    18901 cattttctat ggctggcggg gagtatgtta gtgtttctac tcaaaaagat actgaacaag
    18961 cagcagttgc gcgtgaagag aaactactag aaaataatcc agaacttgcc aaaaaatcct
    19021 tagttgatat ctacttagca aaaggagaaa gtcacgaaca tgctcaatgg ctagttgaca
    19081 aggctttttc aaagaatgct attgagcatt tagttgaaga aaaatatggt attgagttcg
    19141 gtgaatatac aagcccctgg catgctgcta tatctagttt tatagctttt gccatcggat
    19201 caatttttcc aactataact attctgttat tgccattttc ggtacgaatt gtaggaacgg
    19261 taataattgt tattgtttca ctattatcaa ctggttatgt aagtgctaag ttaggtcaag
    19321 ctcctacagt acctgccatg cgtcgaaatg tgatgattgg ttgtttgaca atgttggcaa
    19381 cctacgtcat tggacaatta ttttcaatat aaaatattaa tcgtggagtt attgtctaaa
    19441 ctagaatgga cagtaattct ttttagtttt ataacgaaat taagaagttt ttattgacaa
    19501 aaattggtat ataccatata atataatcat aaaggtctat accaaataaa aggagagtaa
    19561 acatgagagt tattacagtt aagaacgaca ttgagggggg aaaaattgca tttactcttt
    19621 tagaagagaa aatgaaagct ggtgctcaaa ctttaggatt ggcaacagga agtagcccaa
    19681 tcacctttta cgaagagatt gtaaaaagta atcttgattt ttcaaatatg gtttctatta
    19741 accttgatga atatgttgga attgccgcaa gtaatgatca aagttactct tattttatgc
    19801 ataaacatct ttttgatgct aaacctttca aggaaaataa ccttccaaac ggcttggcta
    19861 aagatttgaa agaagaaata aaacgatacg atgctgtcat aaatgctaat ccaattgatt
    19921 ttcaaatcct tggaattggt cgtaacggtc atattggttt taatgaacca ggtacacctt
    19981 ttgatattac aacacatgtg gttgatttag ctccaagtac aattgaagct aatagtcgtt
    20041 tctttaatag tattgatgat gtaccaaagc aagccttatc aatgggtata ggctctatta
    20101 tgaaatcaaa aacaatcgta ttagtggcgt atggtattga aaaagcagaa gcgattgcaa
    20161 gtatgattaa aggacctatc acagaagata tgccagcaag tatcctccaa aaacatgatg
    20221 atgttgtgat tattgtggat gaagcagcgg catcaaagtt gtaaagtaaa taataaaaaa
    20281 gtaaggatga atctcaatga gaaaaaattc attcttactt ttttattatg ctttattaaa
    20341 ttgtccagtc taagttaggt ccagctggaa taataccagt agggttaatg gttttatggc
    20401 taccgtaata gtgttttttg atatgatcaa aattaactgt ctcagcaatt ccaggtaggt
    20461 tgtaaatacg ttttgtataa tgccataaat gtgggtaatc atgtagcgct tttaaattac
    20521 atttaaaatg accataataa acggtgtcaa agcgaactaa agttgtgaaa aggcgaatat
    20581 cagcttcggt aaattgttct ccaacaagat aatggttatc agataagtgt ttttctaact
    20641 gatctaaagc tgtgaataag gtttcaactt ccttttgata aacgttctga gaagtggcaa
    20701 aaccagcttt atagacacca ttatttattt ttggataaat aaaattattc atttcgtcaa
    20761 tttgtccttg taaggaatcg ggatagtaat cgtcagtatt tccagtaata tgattaaatg
    20821 cagtatttaa catacgcata atttcagatg attcattgtt aacgattgta tggaattttt
    20881 tatcccaaag aacagggact gtcacgcgtc ctgtataatc actttgactt gcttgataga
    20941 tttggtaaag gtattggctt tggttaatca tatcaggaat aacgccttta tattcttgaa
    21001 aagtccaacc attctccaac attaaagggt taacaatgga gatagagata tgagattcta
    21061 gatttttaag ctttctcatg attaatacac gtgatgccca agggcaagct agtgaaacgt
    21121 agagatgata tcgtccagat tctgctttaa aacctgcatc cccactaggc cctgctgaac
    21181 catctttagt aacccaatga cggaattgtg taacggttcg gacgaattta ccaccagttg
    21241 atgcagtatc ataccattga tcaacccatt tgccatctac taaaagtccc atgataatct
    21301 cctttgtttt tctattacta attagtagta taaagatttt aaaaagaatt agcaaatgtt
    21361 taacttacag tatggtaaac taagggtatg cgtttagata aattattagg tcaggcagga
    21421 tttgggtcta gaaatcaggt taagaaatta atttgttcta ggcaagtttc tgtagatggt
    21481 caaattgtta caaaggataa tgttattgta gatagtggtc tacaatcgat ttttgtagga
    21541 aaagagaggg tctgcctaaa agaaagttct tattaccttc tttataagcc atcaggagtt
    21601 gtttcagcag ttagagattc agaacataaa acagtcatag acttaattag cgaaaaagat
    21661 aaggtggagg gattgtatcc gattggtcgt ctagatcgtg atacggaagg gcttttaata
    21721 atcacgaata atggtccact gggttaccga atgttacatc caaaacacca cgttgcaaaa
    21781 acatattatg ttgaagtaaa tggtttcttg gaaagagatg ctattacctt ttttgaagaa
    21841 ggagttgtat ttgacgatgg aacaaagtgt aaaccagctg aattaacgat tgatacggct
    21901 aataatgaca aaagtaccgc aagaataact atcacagaag ggaaatttca ccaagtaaaa
    21961 aagatgtttc ttgcatatgg cgttaaagtt atatacttga ggcgtatttc ttttggtgac
    22021 ttgaggcttg atatgaattt gaaaccaggt cagtatcgta gattgagaga ttcagaggca
    22081 gctatcttaa aaaggtattt agattagtga aacgactcac ttgaccttgc ttagtattct
    22141 tcatatactt ataagtgatt attttaaagg agaaaaccat tgaattcaaa actttcattg
    22201 aaattgacta ttttatcgat ttctatcttt ttgatgtctc atttagcaat tgctcctgca
    22261 attcctaaat tgtatgcctt ttaccatact caaaaccctc atattggact agcttcagta
    22321 gagagtttag ttactattcc agctatgatg attactattt ttgttatttt gagtaatttt
    22381 gtagtcacaa aacttggcaa aaaaaacacg gtcttacttg gtttatgttt aatcttaatg
    22441 tctgggttta tctcattttt tacaagtaat ttttcacttg cgatggctag tcgactactt
    22501 ttgggtattg gtattggtct ctataattcg ctatcaatta gtatcataac ggatttatat
    22561 gaggctgatg agagagccag tatgattggt cttagaacag catcactcaa tatcggtaaa
    22621 gccttaacta catttattgt tggattggtt ttagctattg gtgtgaatta tatttatttg
    22681 gtttaccttc tggttattcc tgttttcttt ttcttttgga aaaatgttcc agaggttgaa
    22741 aatcaaacac atacccttaa agctagtaca acatttgata ctaaagcggc tttgttgatg
    22801 ctcatcacgt tcttagtagg tattgcttat ataggagcaa ctgtcaaaat tccaacctta
    22861 ctagtgacaa aatatcacta cgcgacgagc ttctctagta atatgttaac tctccttgct
    22921 tttagtggta ttttagttgg atctgtattt ggaaaattag ttaaagtatt ccaagagaag
    22981 acgctattaa tcatgattct agctatggga ataggtaatg tgctatttgc tttagctaat
    23041 aatcagatca tttttatcgt agctagtatt ttaataggag ctagttttgt tggcaccatg
    23101 tcttctgtat tcttttatat ttcaaaaaac tatgcaaaag aacataataa ctttatcaca
    23161 agccttgcct taacagctgg taatatagga gttattttga caccgcttat tttaacgaaa
    23221 cttccaagcc agctacatct ggaacctttt atgacaccat ttttaataac aagtgggctg
    23281 atggtgatta atgtctttgt ttacctagtt ttaatgtcaa aaaataaata aaaagttatc
    23341 cgaaaggata acttttttcg aatagtaaaa gtaggagaaa aaatgttaat tgctaaagac
    23401 aaacaaggaa atttaattaa tctactcgag tctcatccgg gaaagggaca gtatttttgt
    23461 ccaacttgtt gttcagctgt aaggttaaag gcaggtcgaa tcatgagaag acactttgca
    23521 catattagtc taaaaaattg tcagttttat catgaaaatg agtctaatga gcacttgcaa
    23581 ttaaaagcaa agttatatat gagtttaagt agagagaatg aaactatgct cgagcatcat
    23641 ttgccagaaa ttaatcagat tgcagattta tttgtgaatg agacattggc tttagaagtt
    23701 cagtgtagcc gactatcaga gcagcgactc agggagcgaa caaaagcata tctacaggca
    23761 gactttcaag tgagatggtt actaggggaa aaactctggt taaagcatcg tttaacaaat
    23821 ctacataaac aatttttaca attttctcaa agtatcggat ttcatatttg ggagttagat
    23881 ttgaggttag aagtactgag actaaaatat ttgatttacg aagatttacg cggacatgtc
    23941 tattacttaa gtaaaacttg ccctcttagt ggagacgttc tagcattttt gaagtggcca
    24001 taccaatcaa aaaatctaaa tttttataag gttaaacaag acagaaatat cagagattat
    24061 gtgcgtcagc agttacgcta tggcaatcaa ttttggttac gaaaacaaga aaaggcctac
    24121 ctttctggtc agaatctatt gacccaagag ctaatgatgt tttttcctca aatacagcca
    24181 ccacgtgtag atactgattt ttgtcaaata acaaattctc taacatcgtt ttatcagaat
    24241 tttacaaatt attatcaaaa aaataagaat aacttagacc aaacacttta tccccctgta
    24301 ttttatgata aaatagtcac taagaattaa actggaggat acaaaatgtc agacaatcgc
    24361 tcacatattg aagaaaaata ccaatgggat ttaactactg tctttgcgac tgatgagtta
    24421 tgggaaactg aagtggtaga gcttactcaa gccatcgata atgcaaaggg tttttcaggt
    24481 catctgttgg actcaagcca atctttattg gagatcacgg aggttgaatt agatttatct
    24541 cgccgtttag aaaaagttta tgtctatgct tcaatgaaaa atgaccaaga caccactgtt
    24601 gcgaaatatc aagaatttca agcgaaagcg acagcgctct atgcaaaatt tagtgaaaca
    24661 ttttcgtttt acgagccaga actattgcaa ctttcagagt ctgattacca gtcattcctt
    24721 ttagaaatgc ctgacttgca aaaatatgat catttctttg agaaaatatt tgcaaataaa
    24781 cctcatgtct tatctcaaaa cgaagaagaa ttgttagctg gtgctagtga gatttttgga
    24841 gcagctggtg agacatttga aatccttgat aatgcagata tggtcttccc agttgtaaaa
    24901 aatgcaaaag gtgaggaagt tgagcttaca catggtaatt tcatttccct tatggaatct
    24961 agcgatagga cggttcgaaa agaagcatat caagctatgt atagcacata tgaacaattc
    25021 cagcatactt acgctaaaac attacaaaca aatgtgaaat ctcaaaattt taaggctcgt
    25081 gtgcatcatt atcaatcagc acgccaatca gctctatctg ccaattttat tccagaagag
    25141 gtctacgaaa ctctaattaa gacagttaat catcatttac cgctcttaca tcgttatatg
    25201 aagttgcgtc aaaaagttct tgggctagat gatttgaaga tgtatgacgt atatacaccc
    25261 ctttctcaaa tggatatgag ttttacttat gatgaagctc taaaaaaatc agaggaggtt
    25321 ctagctattt ttggcgaagc atattctgaa cgtgttcatc gtgcttttac tgaacgttgg
    25381 attgatgtcc atgttaacaa aggaaagagg tcaggcgctt attcaggtgg atcttatgac
    25441 actaatgctt tcatgcttct taactggcaa gatactttag ataatcttta tacgctggtg
    25501 catgaaactg gacatagtct acactcgacg tttactcgtg aaaatcaacc ctatgtttat
    25561 ggtgattatt ctattttcct agctgaaatt gcatcaacaa caaatgaaaa tatattaaca
    25621 gaaacccttt tgaaagaagt taaagatgat aaaaatcgct ttgcaattct caatcattat
    25681 ttggacggtt tcaaaggaac aatttttagg caaacacagt tcgctgaatt cgaacatgcc
    25741 attcatgtag ctgatcagga ggggcaagta ctaactagcg aatacttaaa caatctttac
    25801 gctgagctta atgaaaaata ttatggtttg actaaagaag ataatcattt tattcaatat
    25861 gaatgggcaa gaattccaca cttttactac aattattatg ttttccaata tgcaaccggc
    25921 ttcgcagcag caaattattt agcagagcgt attgtaaacg gtaatccgga ggataaagag
    25981 gcctacctaa attaccttaa agctgggaat tcagattacc ctctaaacgt cattgctaaa
    26041 gctggtgttg atatgactag tgcagactat ctggatgcag cttttagagt atttgaagag
    26101 cgtttagtgg aactggaaaa tcttgtagca aaaggcgtcc ataatgatta agttgaaaat
    26161 atagctattg aagtaaaacc agtcaactag actggttttt gatagtgatc agttatttta
    26221 tagtttgttt tgagagtggt tttggtaaag ttagttagtg gaaaactcaa taggtggaaa
    26281 tacctaccca agttatgatt tttatgagac caaaactgct attgtcaaaa aagaagggaa
    26341 ataggatgaa gaagcttact tttatttggg atttagatgg gacattaata gattcgtatg
    26401 taccaattat ggaagctctt gaagaaacct atcgtcattt tggtttaata tttgataaag
    26461 aattaatcca tgaatatatt ttacaggaat cagtggggaa attattggta aacctttcag
    26521 aggaagagca aatacctcat gaaaaactga aagcatattt tacaaaagaa caagaaagtc
    26581 gagattctaa aatacattta atgccatatg caaaagagat tttagaatgg accaaagaac
    26641 aagatatccc caattttatg tatacacata aaggagcaag tacgcattca gtgttggaaa
    26701 ccttgcagat ctctcattat tttgatgaaa ttttaactgg tgtttcggga ttcgagcgaa
    26761 aaccacatcc acaagggatt aattatttag
//
LOCUS       DATRNK010000023        26775 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.023, whole genome shotgun sequence.
ACCESSION   DATRNK010000023 DATRNK010000000
VERSION     DATRNK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26775
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.023"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            44..1177
                     /gene="ugpC"
                     /locus_tag="VBC52_000978"
     CDS             44..1177
                     /gene="ugpC"
                     /locus_tag="VBC52_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEO6882575.1"
                     /translation="MVELNLNHIYKKYPSASHYSVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIDGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKFSKQEIDKRVREAAANIGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHQRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPDGD
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPSMNFFKVKVENGMIISEDGLRIAIPEG
                     QEKLLESRGYKGKELIFGIRPEDISSNLLVQDTYPNANVEAEVLVSELLGSETMLYVK
                     LGQTEFASRVEARDFHNPGEKVNLTFNVAKGHFFDADTEQAIR"
     gene            1306..2913
                     /locus_tag="VBC52_000979"
     CDS             1306..2913
                     /locus_tag="VBC52_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEO6882576.1"
                     /translation="MKKHWWHKATIYQIYPRSFMDSDGDGVGDIKGITSKLDYLEKLG
                     ITAIWLSPVYQSPMDDNGYDISDYQAIADIFGDMNDMDQLLQEANQRGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYLWRDEPNDLTSIFSGSAWEYDKASGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIDKGIGGFRMDVIDLIGKIPDKGITGNGPKLHDYL
                     KEMNRASFGKHDLLTVGETWGATPDIAKQYSNPDNEELSMVFQFEHVGLQHKPDAPKW
                     DYSDGLDVPALKDIFTKWQTQLELGQGWNSLFWNNHDLPRVLSIWGNDSDNRKQSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFECLADVDDIESLNYAKEAMDNGVSEATILDS
                     IRKVGRDNARTPMQWSQEHQAGFTKGTPWLAVNPNYQEINVEAALNDTESIFYTYQKL
                     VALRKEHDWLVDADFKLLETADKVFAYVRQTDKERYLIVANLSDKNQSFEFPEAVKET
                     IISNTEVQEVLSNNTLKPWDAFCIELF"
     gene            3001..3996
                     /gene="galE"
                     /locus_tag="VBC52_000980"
     CDS             3001..3996
                     /gene="galE"
                     /locus_tag="VBC52_000980"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEO6882577.1"
                     /translation="MAVLILGGAGYIGSHMVDQLITQGKEKVIVVDNLVTGHRQAVHS
                     DAIFYEGDLSDKTFMRQVFRENPDVDAVIHFAAFSLVAESMENPLKYFDNNTAGMIKL
                     LEVMNECDIKNIVFSSTAATYGIPEQVPILETAPQNPINPYGESKLMMETIMKWADQA
                     YGIKFVALRYFNVAGDKPDGSIGEDHKPETHLLPIILQVAQGVRDKIMIFGDDYNTPD
                     GTNVRDYVHPFDLADAHILAVDYLRQGNESNVFNLGSSTGFSNLQMLEAARRITGKEI
                     PAQKAARRPGDPDTLIASSEKARQILGWEPKFDNIDKIISSAWAWHSSHPNGYED"
     gene            complement(4017..4697)
                     /locus_tag="VBC52_000981"
     CDS             complement(4017..4697)
                     /locus_tag="VBC52_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO6882578.1"
                     /translation="MDVLIIEDDPMVEFIHRNYLEKLNYFQNIYSTASQTQAIAYLND
                     IKIQLVLLDIHIKEGNGLELLKLLRNQHQNTEVIVISAANEAHTVKEAFHLGIVDYLI
                     KPFTFERFESSIEKFLNHYHTFEADKIYQDNIDHFQKIDSGWLEGEVKLDEKGLSEIT
                     YQHILDAIQELEQPFTIQELAKCSQFSHVSVRKYIAYMEEKGLLTSQQIYTKVGRPYK
                     VYKLIKKT"
     gene            complement(4699..6174)
                     /locus_tag="VBC52_000982"
     CDS             complement(4699..6174)
                     /locus_tag="VBC52_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEO6882579.1"
                     /translation="MTICIFSIFYYVTIHQSYRMVRVQEEKILKNTGYALSRNPQVIQ
                     TLKDNHYDQSLQKQMLFLSKKSNLDYIVLINLKGIRFTHPDSTKIGKPFQGGDEQAVF
                     KGKAIMSTAEGSLGKSLRYLIPVYDHQKQVGAIAVGLKLTTLGDLSQSSIKEFSKPLL
                     ISILISLVVTSIISYGLKKQLHNLHPSDIFQHLEERNATLDQIQAAVFVIDQRHIIKR
                     NNPAASLLFKKEGQRDLFSGKLLESLIPQLKQDHFSKKTEQVLHFQGQDYLLSISPIT
                     VKTQNRGYVVFLRNVTETLFTLDQLAHTTAYASALQAQTHQFMNQLHVIYGLADIEYH
                     DELKIYLKELLEPQNEFLARLSMLVREPRLASFIIGEREKFAEKHINLSTEILVDIPT
                     KSTVEDVNNYLLLHRYINTKILTLLNSTTLVSLRLNYQNNLIETDYQWENEKWLLNDY
                     HQYFNDAYFQQLLVDSRATYKISLKQDKVIIRTNIIYSEGS"
     gene            6378..7715
                     /locus_tag="VBC52_000983"
     CDS             6378..7715
                     /locus_tag="VBC52_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEO6882580.1"
                     /translation="MADVKVVNNEDSRGQKQDLKTKLFHIKVGSVPLPVYVCLALLIL
                     LAGFLQKLPVNMLGGFAVILTMGWFLGTIGASIPGFKNFGGPAILSLLVPSILVFFNL
                     INKNVLESTNMLMKQANFLYFYIACLVSGSILGMNRKMLIQGLLRMIFPMLLGMVCAM
                     MVGTFVGVILGLEWRHTLFYIVTPVLAGGIGEGILPLSLGYSSITGVASEQLVAQLIP
                     ATIIGNFFAILCTALLNRLGEKKPHLSGQGQLVRLNKGEDMSDIIADHSGPIDVKKMG
                     GGVLTACSLFIFGHLLQQLTGFPGPVLMIIAAAILKYINVIPRETQNGAKQLYKFISG
                     NFTFPLMAGLGLLYIPLKDVVATLSIQYFIVVISVVFTVISVGFFVSRFLNMNPVEAG
                     IISACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRMLF"
     gene            7740..8903
                     /locus_tag="VBC52_000984"
     CDS             7740..8903
                     /locus_tag="VBC52_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEO6882581.1"
                     /translation="MSENLGQLAINQARENGGKLEVISKVKVEDKRELSIAYTPGVAS
                     VSSAIAEDVELAYELTTKKNTVAVVSDGSAVLGLGDIGPEAAMPVMEGKAALFKRFAN
                     VDAVPIVLKTNDTEEIISIVKAISPTFGGINLEDISAPRCFEIEQRLIEECDIPVFHD
                     DQHGTAIVVLAALFNSLKLVKKDIEDIRVVVNGGGSAGLSITRKLLSAGAKHVTVVDR
                     FGIINDKDRESLAPHHKAIAKLTNREFQSGSLEDALENADVFIGVSAPEALHAEWISK
                     MADKPIVFAMANPIPEIYPDQALKVGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAARGIASLIPEEELSTTHIIPNAFQNDVADVVAKSVSNAVQK"
     gene            9094..9438
                     /locus_tag="VBC52_000985"
     CDS             9094..9438
                     /locus_tag="VBC52_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEO6882582.1"
                     /translation="MYSFEELLATMTLITAAEIEDKIDSNQDFVLFIGRISCPFCHLF
                     VPKIVEVADEDEFELFHLDSEDFDHWTANKEFRNKYDIPTVPGLMVVKNGTIKVKCDS
                     KMTKEEIREFIG"
     gene            9515..9856
                     /gene="yajC"
                     /locus_tag="VBC52_000986"
     CDS             9515..9856
                     /gene="yajC"
                     /locus_tag="VBC52_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEO6882583.1"
                     /translation="MSLPIIMLVVMVGMMFFMQRQQKKQAQERQKQLNAVQKGDEIVT
                     IGGLFGVVDEVNTEAQRMVLDVDGVYLTFELAAIKSVVSKAATPTEPVEDVKIIEVNE
                     DENKETAIETE"
     gene            10023..10775
                     /locus_tag="VBC52_000987"
     CDS             10023..10775
                     /locus_tag="VBC52_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEO6882584.1"
                     /translation="MFIFKRKKQVEMPLEKIPAHIGIIMDGNGRWAKKRLKPRVMGHK
                     AGMDALQEVTIAASELGVKVLTVYAFSTENWSRPDDEVKFIMNLPVEFFDKYVPELDK
                     NNVRVQVIGDTHKLPKATYDAMQRACLRTKHNSGLVLNFALNYGGRSEITNAIKEIAQ
                     DVLEAKLNPDDITEDLVANHLMTNSLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEF
                     YFTPVLWPDFKKDELHKAIVDYNQRHRRFGSV"
     gene            10790..11584
                     /locus_tag="VBC52_000988"
     CDS             10790..11584
                     /locus_tag="VBC52_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEO6882585.1"
                     /translation="MKERVIWGAVALAIFIPFLVMGGLPFQFLVGLLAMIGVSELLRM
                     RRLEIFSFEGALAMIGSFVLTVPLDSYLSFLPVDASLSAYGIVIFMILAGTVLNSNSY
                     SFEDAAFPIASSFYVGIGFQNLVSARMAGIDKVLLALFIVWATDIGAYMIGRQFGQRK
                     LLPSVSPNKTIEGSLGGIASAIVVAFFFMLFDKTVYAPHSFLVMLVLVAIFSIFGQFG
                     DLVESSIKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHFFGLF"
     gene            11615..12874
                     /gene="rseP"
                     /locus_tag="VBC52_000989"
     CDS             11615..12874
                     /gene="rseP"
                     /locus_tag="VBC52_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEO6882586.1"
                     /translation="MLGILTFIIIFGVIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHIDKEGTTYTIRILPLGGYVRMAGWGDDKTEIKTGTPASLTLNKEGIVTRINLSGK
                     QLDNTSLPINVTAYDLEDKLTITGLVLSETKTYSVDHDATIIEEDGTEIRIAPLDVQY
                     QNASVWGRLITNFAGPMNNFILGLVVFIALAFIQGGVQDLSTNQVRVSENGPAASAGL
                     KNNDRILQIGSHKVSNWEQLTAAVEKSTRHLEKKQKLALKIKSKEVVKTINVKPQKVD
                     KSYIIGIMPALKTSFKDKLLGGFKLAWESFFRILNELKKLIAHFSINKLGGPVALYQA
                     SSQAAKNGFVTVLNLMGLISINLGIMNLIPIPALDGGKIVMNILEAIRRKPLKQETET
                     YITLAGVAVMLVLMIAVTWNDIMRAFF"
     gene            12966..14819
                     /locus_tag="VBC52_000990"
     CDS             12966..14819
                     /locus_tag="VBC52_000990"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEO6882587.1"
                     /translation="MKQSKMLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMRQEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDQS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHKDYEDLDVTYEDYRKAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAVTPNRT
                     DLNRWLVLDKTIPSIDDIPEDVLEEIKAELSAWLVSGEDTIAYSTESSYAANLEMATN
                     EYKPSTKAATFEEVTKVETPNCKSIDEVAGFLSIDENQTIKTLLFIADEQPVVALLVG
                     NDQVNDVKLKNYLAADFLEPASEEQAKEIFGAGFGSLGPVNLPESVKIIADRKVQDLA
                     NAVSGANQDGYHFTGVNPERDFTAEYVDIREVKEGEISPDGKGTLKFARGIEIGHIFK
                     LGTRYSDSMGANILDENGRSNPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGAYRF
                     AWGINFPEELAPFDVHLITVNVKDQESQDLTEKIEADLMLKGYEVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKASEGIVEVKIKASGDTIEVHADNLIETLEILTKK"
     gene            14944..15528
                     /locus_tag="VBC52_000991"
     CDS             14944..15528
                     /locus_tag="VBC52_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEO6882588.1"
                     /translation="MKSRKKDKLVLRLTTTLLVFGLGGVWFYNYKNDNVEPTVTSASD
                     QTTTFIQTISPTAIEISKTYDLYASVLLAQAILESSSGQSDLSKAPNYNLFGIKGEYK
                     GKSVQMPTLEDDGKGNMTQIQAPFRAYPNYSASLYDYAELVSSQKYASVWKSNTSSYK
                     DATAALTGLYATDTAYASKLNQIIENYSLDAYDK"
     gene            15651..20057
                     /locus_tag="VBC52_000992"
     CDS             15651..20057
                     /locus_tag="VBC52_000992"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEO6882589.1"
                     /translation="MSELFKKLMDQIEMPLEIKNSSVFSSADIIEVKVHSLSRLWEFH
                     FSFPELLPIEVYRELQTRLVNSFEKADIKATFDIRAETIDFSDDLLQDYYQQAFCEPL
                     CNSASFKSSFSQLKVHYNGSQMIISAPQFVNNNHFRQNHLPRLEQQFSLFGFGKLAID
                     MVSDEQMTQDLKSSFETNRERLLEKANQEAMQALETQKSLEDSAPPSEEVTPTQNYDF
                     KERIKQRQAGFEKAEITPMIEVTTEENRIVFEGMVFSVERKTTRTGRHIINFKMTDYT
                     SSFAMQKWAKDDEELKKYDMISKGSWLRVRGNIENNNFTKSLTMNVQDIKEIVHHERK
                     DLMPADQKRVEFHAHTNMSTMDALPTVESLIDTAAKWGHPAIAITDHANVQSFPHGYH
                     RAKKAGIKAIFGLEANIVEDKVPISYNEVDMNLHEATYVVFDVETTGLSAANNDLIQI
                     AASKMFKGNIIEQFDEFIDPGHPLSAFTTELTGITDNHVRGSKPILQVLQEFQNFCQG
                     TVLVAHNATFDVGFMNANYERHNLPLITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQ
                     VALEHHHMANYDAEATGRLLFIFLKEARENRDVTNLMELNTKLVAEDSYKKARIKHAT
                     IYVQNQVGLKNIFKLVSLSNVKYFEGVARIPRSVLDAHREGLLLGTACSDGEVFDALL
                     SNGIDAAVTLAKYYDFIEVMPPAIYRPLVVRDLIKDEAGIQQIIRDLIEVGRRLDKPV
                     LATGNVHYIEPEDEIYREIIVRSLGQGAMINRTIGRGEDAQPAPLPKAHFRTTNEMLD
                     EFAFLGKDLAYEIVVTNTNTFADRFEDVEVVKGDLYTPFVDRAEERVAELTYAKAFEI
                     YGNPLPDIIDLRIEKELASILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVA
                     TMIGITEVNPMPPHYVCPNCQHSEFITDGSCGSGYDLPNKNCPKCGTLYKKDGQDIPF
                     ETFLGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGEEYAFRAGTVGTVAEKTAFGFVK
                     GYERDYNKFYNDAEVERLATGAAGVKRSTGQHPGGIVVIPNYMDVYDFTPVQYPADDM
                     TAAWQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPSNILPDDPDVMKLFS
                     GTEVLGVTEEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWL
                     GNAQDLIKEGIATLSTVIGCRDDIMVYLMHAGLQPKMAFTIMERVRKGLWLKISEDER
                     NGYIQAMRDNNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHYPIFYYCAYF
                     SIRAKAFELRTMSAGLDAVKARMKDITEKRQRNEATNVENDLFTTLELVNEMLERGFK
                     FGKLDLYRSHATDFIIEEDTLIPPFVAMEGLGENVAKQIVRAREDGEFLSKTELRKRG
                     GVSSTLVEKFDEMGILGNLPEDNQLSLFDDFF"
     gene            20183..20608
                     /locus_tag="VBC52_000993"
     CDS             20183..20608
                     /locus_tag="VBC52_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6882590.1"
                     /translation="MGNFKNSAVKSMVVMRKAFRTIDGKVSESFKEFELTPTQFAVLD
                     VLYAKGTMKIGELIENMLATSGNMTVVIKNMEKKGWVLRHSCPNDKRAFLVSLTTEGE
                     EVIKKALPEHIKRVEDAFSVLTETEQEDLINLLKKFKTL"
     gene            20741..21355
                     /locus_tag="VBC52_000994"
     CDS             20741..21355
                     /locus_tag="VBC52_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO6882591.1"
                     /translation="MSFLEELKNRRSIYALGRNTEVSNEKIVEIIKEAVRQSPSAFNS
                     QTSRVVILLNDEVTKFWDELVANDLVETMKVQGAPETAIAGTKEKLASFGASKGTVLF
                     FEDQDVVKSLQEQFVLYADNFPVWSEQSTGIASVNTWTALSAELGLGGNLQHYNPVID
                     ASVQAVYGVPASWKLRGQLNFGSIEAETGEKEFMNDDDRFKVIG"
     gene            complement(21402..22040)
                     /locus_tag="VBC52_000995"
     CDS             complement(21402..22040)
                     /locus_tag="VBC52_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO6882592.1"
                     /translation="MIKSKILLQLMQGKIVAVIRGNSQEEAFQAAQACIKGGISAIEI
                     AYTNSKASQVIEQLVTQYTNQEQVVVGAGTVLDSETARMAILAGAKFIVSPAFNLQTA
                     KLCNRYAIPYLPGCMTLSEITTALEAGCEIIKIFPGGTLGTSFISSLKAPLPQVQIMV
                     TGGVNLTNAKDWFLSGVTAIGIGGEFNKLAALGEFDKITEMAKQYKIQLNLN"
     gene            complement(22052..23059)
                     /locus_tag="VBC52_000996"
     CDS             complement(22052..23059)
                     /locus_tag="VBC52_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO6882593.1"
                     /translation="MSKILFFGEPLIRITPKENDYFADSISTKLFYGGSEVNIARALQ
                     GFGQDTKLLSALPNNPIGNSFLQFLKAQGIDTHSIQWVGERVGLYFLENSFACRKGEV
                     VYDRDYSSLHDFRINQIDFDQLFEGVSLFHFSGITLSLDESIQEITLLLLKEAKKREI
                     TISLDLNFRSKLISPKNAKILFSQFATFADICFGIEPLMVDSQDTTFFNRDEATIEDI
                     KERMISLINHFDFQVIFHTKRLQDEWGRNHYQAYIANRKQEFVTSKEITTAVNQRIGS
                     GDAFVAGALYQLLQHSDSKTVIDFAVASASLKCALEGDNMFETVAAVNKVLNQSKDII
                     R"
     gene            complement(23085..23726)
                     /gene="dhuI"
                     /locus_tag="VBC52_000997"
     CDS             complement(23085..23726)
                     /gene="dhuI"
                     /locus_tag="VBC52_000997"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase DhuI"
                     /protein_id="HEO6882594.1"
                     /translation="MKIALINENSQASKNTIIYKELKAVSDKKGFDVFNYGMYGKEEE
                     ESQLTYVQNGLLTAILLNSGAADFIITGCGTGIGAMLACNSFPGVVCGFAADPVDAYL
                     FSQVNGGNALSLPFAKGFGWGAELNLRYLFERLFEDEKGGGYPKERAVPEQRNARILS
                     EVKQITYRDLLSVLKEIDQDFLKETISGEHFQEYFFANCQNQNIADYLKSVLD"
     gene            complement(23743..24555)
                     /gene="dhuD"
                     /locus_tag="VBC52_000998"
     CDS             complement(23743..24555)
                     /gene="dhuD"
                     /locus_tag="VBC52_000998"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric 2-dehydro-3-deoxy-D-gluconate
                     5-dehydrogenase DhuD"
                     /protein_id="HEO6882595.1"
                     /translation="MTEQFLKDNFSLEGKVALITGASYGIGFSIATAFARAGATIVFN
                     DIKQELVDKGISAYKKLGIKAHGYVCDVTDEDGINEMVNKISQDVGVIDILVNNAGII
                     KRTPMLEMSAADFRQVIDIDLNAPFIVSKAVLPGMIQKGHGKIINICSMMSELGRETV
                     AAYAAAKGGLKMLTKNIASEYGSANIQCNGIGPGYIATPQTAPLRERQDDGSRHPFDQ
                     FIIAKTPAARWGEAEDLGAPAIFLASDASNFINGHILYVDGGILAYIGKQPQ"
     gene            24834..25268
                     /locus_tag="VBC52_000999"
     CDS             24834..25268
                     /locus_tag="VBC52_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6882596.1"
                     /translation="MIKIIIVAHGNFPDGILSSLELIAGHQEYVAGINFIAGMSSNDV
                     RVALQREVIDFKEILVLTDLLGGTPFNVSSALSVEYTDKKIKVLSGLNLSMLMEAVLS
                     RTMFEHVDDLVDKVITSSREGIVDFSNCLATQIAEVTFEGGI"
     gene            25271..26467
                     /locus_tag="VBC52_001000"
     CDS             25271..26467
                     /locus_tag="VBC52_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEO6882597.1"
                     /translation="MMKIKPVKVESIENPKRFLDGRLLTKIEVEGAIEKALKQLYINI
                     DYFGEEYPTPATFNNTYKVMDNTEWTNGFWTGCLWLAYEYNQDKKLKNIAHKNVLSFL
                     NRINNRIALDHHDLGFLYTPSCTAEYRINGDVKALEATIKAADKLMERYQEKGGFIQA
                     WGELGYKEHYRLIIDCLLNIQLLFFAYEQTGDEKYRQVAVNHFYASANNVVRDDSSAF
                     HTFYFDPETGEPLKGVTRQGYSDESSWARGQAWGIYGIPLSYRKMKDYQQIILFKGMT
                     NYFLNRLPEDKVSYWDLIFTDGSGQPRDTSATATAVCGIHEMLKYLPEVDPDKETYKY
                     AMHTMLRSLIEQYSNNELIAGRPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYK
                     DWELYW"
     gene            26522..>26775
                     /locus_tag="VBC52_001001"
     CDS             26522..>26775
                     /locus_tag="VBC52_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6882598.1"
                     /translation="MAAGPNIVMTRVDERLIHGQGQLWVKFLSCNTVIVANDDVSKDH
                     LQQTLMKTVVPESIALRFFDIQKVIDIIHKANPAQTIFIIV"
BASE COUNT         8465 a         4325 c         5426 g         8559 t
ORIGIN      
        1 tctttgttat aataaaatca caataataaa agaggtcaat ctaatggtag aattaaattt
       61 aaatcatatt tacaaaaaat atccaagtgc atcgcattat tctgttgaag actttgactt
      121 ggatattaaa gacaaagaat ttattgtttt tgttggacca tcaggttgtg ggaaatcaac
      181 aacacttcgt atgatcgcag gtcttgaaga tattagtgaa ggtgagctca agattgatgg
      241 cgaagttgtc aatgacaaat cacctaaaga tcgtgatatt gctatggttt tccaaaacta
      301 tgctctttat ccacatatga ctgtttacga taatatggct tttgggctca aattgcgtaa
      361 attctcaaaa caagaaattg ataagcgcgt tcgtgaagca gcagctaata ttggccttac
      421 tgaattctta gagcgcaaac ctgcggattt gtcaggtgga caacgccaac gtgttgctat
      481 gggacgtgct attgtgcgtg atgccaaagt gttcttaatg gatgagcctt tatctaactt
      541 agatgctaaa ttacgtgttt caatgcgtgc tgaaattgca aaaattcatc aacgtattgg
      601 ttcaacaact atttatgtta cccacgacca aacagaggcg atgactttgg cagaccgtat
      661 tgtcattatg tcagctacta agaaccctga tggtgatggt acaattggta aaattgagca
      721 agttggttca ccacaagaac tctataatct tcctgccaat aaatttgttg cgggattcat
      781 tggtagccca tctatgaact tcttcaaagt taaagttgaa aatggtatga ttattagtga
      841 agatggtctt cgaatcgcta tccctgaagg tcaagaaaaa cttttagaat cacgaggcta
      901 taaaggaaaa gaattgattt ttggtattcg tccagaagac atttcaagca atcttcttgt
      961 ccaagatact tatccaaatg ccaatgttga agcagaagtt ttagtttctg aattactcgg
     1021 tagcgaaact atgctttatg ttaagttagg tcaaacagaa tttgcttctc gtgttgaagc
     1081 gcgtgacttc cataatccag gagaaaaagt taacctaacg tttaatgttg ctaaggggca
     1141 tttctttgat gcagatactg aacaagctat tcgttaaggt ataagcgata ttcataaaaa
     1201 ggttgggttc cagccttttt ataatgttat ttgcgcaaac gcttgcactg aaagaggaaa
     1261 aacgttaaaa taaaagatga atgaattatt gataggagaa gaataatgaa gaaacactgg
     1321 tggcataaag ctacgattta tcaaatttac ccacgttcat ttatggattc agatggtgat
     1381 ggtgtggggg acattaaagg gattactagc aaactagact accttgaaaa attagggatt
     1441 actgctatct ggttatcacc tgtttatcaa agtccaatgg atgacaatgg ttatgatatt
     1501 tcagattatc aagccattgc agatattttc ggtgacatga atgatatgga ccaactgtta
     1561 caagaggcaa atcaacgcgg gattaaaatc atcatggatt tggtagtgaa tcatacctct
     1621 gatgaacatg cttggttcgt agaagcgcga gaaaacccta atagtcctga acgtgatttt
     1681 tatctttggc gagatgaacc taacgatttg acttctattt ttagtggctc agcttgggaa
     1741 tatgataaag catcaggtca atattatttg caccttttta gcaaacgtca gccagatctc
     1801 aactgggaga atgaagcttt acgtcataaa atttatgata tgatgaattt ctggattgat
     1861 aagggtattg gtggtttccg tatggatgtc attgatttaa ttgggaaaat tccagataag
     1921 ggcattactg gtaatggacc aaaactccat gattatctta aagaaatgaa cagagctagt
     1981 tttggaaagc atgacttatt gacggtgggt gaaacttggg gagcaacgcc agacattgca
     2041 aaacaatact caaatcccga taatgaagaa ttatctatgg tctttcaatt tgaacatgta
     2101 ggtcttcaac ataaacctga cgctcctaag tgggattaca gtgacggatt agatgttcca
     2161 gcattgaaag atatttttac aaaatggcaa acacaattgg agcttggcca aggttggaat
     2221 tctttattct ggaataatca cgaccttcct cgggtgctct cgatttgggg aaatgatagt
     2281 gataacagaa aacaatctgc caaggctcta gcaatcctac tacatttgat gcgtggaacg
     2341 ccttacatct atcaaggtga agaaattggt atgaccaatt atccatttga gtgtttagca
     2401 gatgtagatg atattgaatc ccttaactat gctaaagaag ccatggataa tggggtatct
     2461 gaagcaacaa ttttagatag tattcgtaaa gtcggtcgtg ataatgctcg tactccaatg
     2521 cagtggagtc aagaacatca agctggtttt actaaaggta ccccatggct agcagtcaat
     2581 cccaattatc aggaaattaa tgtggaggca gcgctaaatg atacggaaag tattttttat
     2641 acttatcaaa aattagttgc tttacgtaaa gaacacgact ggttagttga tgctgatttt
     2701 aaacttttgg aaacagcaga taaggtattt gcttatgttc gtcaaacaga taaagaacgt
     2761 tatttaatag ttgcgaactt atccgataaa aaccaatctt ttgaatttcc agaagctgtt
     2821 aaagaaacta ttatttcaaa tacagaggtt caagaagtac tatcaaataa cactcttaag
     2881 ccgtgggatg ccttctgcat agagctattc taatttcggt agatatttgc aaaaaaagct
     2941 tattctcagt ataatgaggt aacaaatgat gttaaaagag tattgaaata ggagaaaaat
     3001 atggcagttc tgattttagg tggcgccggc tacattggct cgcatatggt tgatcagtta
     3061 atcacacaag gaaaagagaa agttattgtt gttgataact tggtaacagg tcatcgtcaa
     3121 gcggtgcatt cagatgcgat tttttatgaa ggagatttat ccgataaaac gtttatgcgt
     3181 caagttttta gggaaaatcc tgatgtagat gcagttatcc attttgcggc attttcacta
     3241 gttgctgaat caatggaaaa tcctttaaaa tattttgata ataacactgc tggaatgata
     3301 aaacttttag aagtaatgaa tgaatgtgat atcaaaaata ttgtcttttc atcaacagct
     3361 gcaacatatg gtattccaga acaagttcct attttagaaa cagctcctca aaatcctatt
     3421 aatccttatg gcgaaagtaa gcttatgatg gaaacaatta tgaaatgggc tgatcaagcc
     3481 tacggtatta agtttgtcgc tctacgttac ttcaacgttg ctggagataa accggatggc
     3541 tcaattgggg aagatcataa accagaaaca catttgttac caatcattct tcaagttgct
     3601 caaggagtac gtgacaaaat aatgattttt ggagatgatt acaatactcc agatggaact
     3661 aatgttcgag attatgtgca tccatttgat ttggcagatg ctcatatatt agcagttgat
     3721 taccttcgcc aagggaatga atcaaacgtg tttaatctcg gatcttcgac aggtttttct
     3781 aaccttcaga tgttagaggc agctcgtcgt attactggga aagaaattcc tgctcaaaag
     3841 gcagctcgtc gtccaggaga tccagatacg cttattgctt cctcagagaa agctcgtcaa
     3901 atccttgggt gggagcctaa atttgataat attgataaaa ttatttcatc ggcatgggca
     3961 tggcactcta gtcatccaaa tggctacgaa gattaaaaaa acttaggtct acaggcctaa
     4021 gtttttttta taagcttata aactttgtaa ggacgaccga ccttagtata aatttgctga
     4081 cttgtcagaa ggcctttttc ttccatatag gcaatatatt tcctaacaga gacatgagaa
     4141 aattgactgc attttgctaa ctcttggata gtgaaaggct gctctaactc ttgaatggca
     4201 tcaagaatat gctgataggt aatttcagag agccctttct catcaagttt aacctctccc
     4261 tctagccatc cagaatctat tttttgaaag tgatcgatgt tgtcttggta gattttatca
     4321 gcttcaaatg tatgataatg atttaggaat ttttcaatac tagactcaaa acgttcaaat
     4381 gtaaatggtt ttatcaggta atctacaata cctaaatgaa atgcttcttt gacagtgtgc
     4441 gcctcgttgg ctgctgaaat aacaatgact tcggtatttt ggtgttgatt gcgtaataac
     4501 tttaataatt ctagaccatt gccttcttta atatgaatat ctaaaagaac aagttggatt
     4561 ttaatgtcat taagataagc aatagcttgt gtttgactag cagttgaata gatgttttga
     4621 aaataattga gtttttcaag gtaattgcgg tggataaatt ccaccatagg atcatcttcg
     4681 ataattaata cgtccatact aagacccttc tgagtagata atatttgttc ttataataac
     4741 cttatcttgt tttaaagaga tcttataagt agctcttgaa tctacaagta actgttgaaa
     4801 ataagcgtcg ttgaaatact gatggtagtc attgagtaac cacttctcat tctcccattg
     4861 ataatctgtt tcaatgaggt tattttgata atttaaacgt aaagaaacta aagtcgttga
     4921 atttaatagc gtgagtatct tcgtattaat atatctgtgg agcaagagat agttattaac
     4981 atcctcaaca gttgacttgg tagggatgtc tactaaaatt tctgtggata gattgatgtg
     5041 tttttctgcg aatttttctc tttcgccaat aatgaaacta gccagtcgag gctctctaac
     5101 taacattgat aagcgtgcta aaaattcatt ttgtggctcc aataattctt ttaaataaat
     5161 tttcaactcg tcgtggtatt cgatgtctgc aagtccataa ataacatgaa gttgattcat
     5221 aaattgatgt gtttgggcct gtagagcaga agcataagct gtagtgtgtg ctaattgatc
     5281 caaagtgaaa agtgtttcag taacattcct taagaagaca acatagcctc tattttgtgt
     5341 tttaaccgta attggcgaga tagatagtaa atagtcttgt ccttgaaaat gtaatacttg
     5401 ttcagttttt tttgaaaaat gatcctgctt gagttgagga attaaagatt ctaataattt
     5461 acctgaaaat aggtctcgtt gcccctcttt tttgaataat aaactagctg caggattatt
     5521 tcttttgatg atatgcctct ggtcaataac aaatacagcg gcttgaattt ggtccagtgt
     5581 tgcattgcgc tcttctaaat gttgaaaaat atcacttgga tggaggttgt gaagttgttt
     5641 ttttaagcca taagaaatta tacttgtgac gactaagcta atcaaaatac taatcaagag
     5701 aggcttacta aactctttta tgctgctttg agataagtct ccaagtgtcg ttaattttaa
     5761 gccaactgca attgccccaa cctgtttttg gtgatcatat actggaatta agtatcgtaa
     5821 agattttcct aaagaacctt ctgcagtaga cataatagct tttcccttaa aaacagcttg
     5881 ttcatcaccc ccttgaaatg gtttaccaat cttagtggag tcagggtggg taaaacggat
     5941 cccttttaaa tttataagga caatatagtc taaatttgat tttttgctaa ggaatagcat
     6001 ttgtttttgg agactttgat catagtgatt atctttgagt gtttgtataa cttgtggatt
     6061 tctcgataaa gcataaccag tattttttaa gattttttct tcctgtaccc ttaccatacg
     6121 ataggactga tgtatagtga cataataaaa aatagaaaat atacaaatgg tcatagtgac
     6181 taaaatcaat gatagaaatg cccaaagaga tagttttttt tcatcatcac cctccgaatt
     6241 tagtataaca tagcctaaaa actagaatcc agcttaaatt aattaaataa gttgaaataa
     6301 ttcaattact taaataaact tccttatcac taagtcccaa agtaaactgt acataagaag
     6361 taaaaggagg tgctttcatg gcagacgtga aggtagtgaa taatgaggat tctagaggtc
     6421 aaaagcaaga cttaaagact aaactatttc atattaaggt agggtcagtt cccctaccag
     6481 tatatgtttg tttagcatta ttgattcttc tagcaggctt tttacaaaaa ttgccagtca
     6541 atatgctagg aggatttgca gtcatcttaa caatgggatg gttcttaggt actattggag
     6601 ctagcatccc tggttttaaa aacttcggtg gcccagctat tttatcttta ttagtaccat
     6661 ctattttggt gtttttcaac ctcattaata aaaatgtttt agaatcaaca aatatgttga
     6721 tgaagcaagc taactttctt tatttttata ttgcttgttt agtgtccggt agtattttag
     6781 ggatgaatcg gaaaatgttg attcagggat tgctaagaat gattttcccc atgttattag
     6841 gaatggtttg tgcgatgatg gtagggacat ttgtcggtgt tattttaggc ttagagtggc
     6901 gacacacttt gttttatatc gtaacacccg ttttagctgg tggtattggt gaaggtattt
     6961 taccattatc gttaggctat agttcaatta ccggtgtagc tagtgaacaa ctagttgctc
     7021 aactcatccc agccactatt attggtaatt tctttgccat tttatgtact gcactattga
     7081 atcgtttggg agaaaagaaa ccacacttgt ctggtcaagg gcaattagta aggttaaata
     7141 aaggagagga catgtcagat attattgctg atcattctgg cccaattgac gttaagaaaa
     7201 tgggtggagg tgttttaaca gcatgtagtc tctttatttt tggacatttg ttgcagcaat
     7261 taactggatt tcctggccct gtattaatga ttattgcagc agctattttg aaatatatca
     7321 atgttattcc tagagaaaca caaaatggag ctaagcaact ttataaattt atttctggta
     7381 attttacatt tcctctaatg gcagggctag gattgcttta tatcccgtta aaagatgttg
     7441 tggcaacgct tagcatacaa tatttcatag ttgttattag tgttgtattt acagttattt
     7501 ctgttggatt ctttgtatcg cgattcctta atatgaatcc tgttgaagca ggtattattt
     7561 cagcttgtca aagtggtatg ggaggaacag gagatgttgc cattttaagt acagcagacc
     7621 gaatgaactt gatgccattt gctcaagttg ctacgcgttt aggaggagct attactgtta
     7681 tcacaatgac agccatttta cgcatgttat tctaatattt atcaaaagga gttatcaaaa
     7741 tgagtgaaaa tttaggtcaa ttagctatca accaagctag agaaaatggt ggaaaattag
     7801 aagtaatttc aaaagttaag gtggaagata agagggagct tagtattgcc tataccccag
     7861 gggtggcgtc agtatcatcg gctattgcag aggatgtgga gcttgcatat gagttgacga
     7921 ctaaaaaaaa tacagtagcc gtagtgagtg atggttcagc tgttcttgga ttaggagata
     7981 ttggtcctga ggcagcaatg ccagtcatgg aaggtaaagc agcactcttt aaacgttttg
     8041 caaatgtaga tgctgtgcca attgtgttga aaacaaatga tacagaggaa attatttcta
     8101 tagtaaaagc gatttcgccg acttttggag ggattaattt agaggatatt tcagcaccac
     8161 gttgtttcga aattgaacaa cgcctaattg aagaatgtga tataccagtt ttccatgatg
     8221 accaacatgg tacagcaatc gtagtattag cggctttgtt taacagtttg aagctagtta
     8281 aaaaagacat tgaagacatc agagtagttg ttaatggtgg aggttcagcg ggcttatcta
     8341 taacaaggaa gcttctatca gcaggagcaa aacatgtaac ggtagttgat cgatttggca
     8401 ttattaacga taaagatcgg gagtcattag cccctcatca caaagcgatt gcaaaattga
     8461 ctaatagaga attccaatct ggatccttag aagatgcttt ggagaatgcc gatgttttta
     8521 taggtgtttc tgctccggaa gcattacatg cagaatggat ttccaagatg gctgataaac
     8581 ctattgtatt tgcaatggcg aatcccattc cagaaattta tccagatcaa gccttaaaag
     8641 tcggagctta catagttgga actggtcgta gtgattttcc aaatcaaatt aataatgttt
     8701 tagcatttcc gggtattttc agaggagcat tagatgctag agcaaaaacg ataactgttg
     8761 aaatgcaaat tgcagcagcg agaggcatag ctagtttaat accggaggaa gaattaagca
     8821 caactcacat cattccaaat gcttttcaaa atgatgtggc cgatgtagtt gctaaaagcg
     8881 tcagtaatgc tgtacaaaaa taaggtgtaa aataaaaact gagctatgct cagttttttt
     8941 gatgagtcat ttaaatatga gtgagcattt ttgaggatag tttgaagcga gaagtgagtt
     9001 aatgattttg atagaatcgt tgttactctt gccttgcaag ttttgccatc ataggttatt
     9061 ctttaaatat aagatttaga aagttggtta actatgtatt cttttgaaga acttttggca
     9121 accatgactt tgataacggc tgccgaaatt gaagataaaa ttgattcaaa tcaagatttt
     9181 gtactgttca ttggtcgtat ttcttgtcca ttttgtcacc tctttgttcc taaaattgta
     9241 gaagtagctg atgaagatga atttgaatta ttccatttag atagtgaaga ttttgatcat
     9301 tggactgcaa ataaagaatt tcgaaataaa tatgacattc ctacagtgcc aggtttgatg
     9361 gttgttaaaa acggaactat caaggtgaag tgcgattcaa aaatgacaaa agaagaaatc
     9421 cgtgaattta ttggctaaga gacagtatct ccttgcagat atagcataaa tctgctatag
     9481 taatgattac gtagaacacg gaaaggagaa tcaaatgagc ttacctatta ttatgcttgt
     9541 tgttatggtt ggtatgatgt tcttcatgca acgtcagcaa aagaaacaag cacaagaacg
     9601 tcaaaaacaa ttgaatgctg ttcaaaaagg tgatgaaatt gtcacaattg gtggtctttt
     9661 tggagtagtt gatgaagtta atactgaagc acaacgaatg gttttagatg ttgatggtgt
     9721 ctatttgacg tttgagttgg cagctatcaa atctgttgtc tctaaggcag caacaccaac
     9781 agaaccagtt gaagatgtta agattattga agtcaatgaa gacgaaaata aagaaacagc
     9841 catcgaaact gaataagata tgtaggacca gatgattgtt ctggtctttt tagtttgctg
     9901 tcacaaatgg cattttaata ctcaaaaata agttaaaatg tggtaaaatg ttatagtatg
     9961 ggatatttcc agctctttta aaagagctga taagaaaaac gattgctaaa ttaaggaaca
    10021 ttatgtttat atttaaaaga aaaaaacaag tagaaatgcc tttggagaaa ataccagcac
    10081 atattggtat tatcatggat ggtaacggac gttgggcaaa aaaacgttta aaaccacgcg
    10141 ttatgggaca taaggcgggg atggatgctc ttcaagaagt tacaatagca gcttcagaat
    10201 taggtgttaa agtactaact gtctatgcct tttctacaga aaattggtca cgtccagatg
    10261 atgaagttaa gtttattatg aaccttccgg ttgaattttt tgataaatat gtaccagagt
    10321 tagataaaaa taatgtacgt gtacaggtca tcggagatac tcataaatta cctaaggcaa
    10381 catacgatgc aatgcaacgc gcttgtttga ggacaaaaca taattcgggg ctcgtcttga
    10441 attttgcgct aaattatggc ggacgttcag aaatcactaa tgccattaag gaaattgctc
    10501 aggatgtttt ggaagctaag ttgaatcctg acgatattac agaggattta gtagcaaatc
    10561 atttaatgac aaatagctta ccctatcttt atagagaccc tgatttgatt atccgaacta
    10621 gtggagagtt acgcttgagt aactttttac cttggcaatc agcttatagt gaattttact
    10681 ttactcccgt tttatggcca gattttaaaa aagacgaact acataaggcc attgtggatt
    10741 ataatcaacg ccatcgtcga tttggtagtg tatagaaaga agagagaaaa tgaaagaacg
    10801 tgttatttgg ggagctgtcg cgcttgccat atttatccct tttttagtaa tgggtggttt
    10861 acctttccaa tttttagttg gacttttagc tatgattggt gtttctgaat tacttcgtat
    10921 gcgtcgttta gaaatatttt cctttgaagg tgcattagct atgataggat cctttgtttt
    10981 aacagttcct ttagatagtt acttgtcctt tttaccagtt gacgctagtt tatcagcata
    11041 cggtattgtt atttttatga ttttggcagg tactgttttg aacagtaatt cttacagttt
    11101 tgaagatgct gcctttccaa ttgcgtctag tttttatgtg ggtatcggct ttcaaaatct
    11161 agtcagtgct cgtatggctg gaatagataa ggtgttgtta gctttattta ttgtctgggc
    11221 gactgatatc ggtgcttata tgattggtcg tcaatttgga caacgcaaac tactaccctc
    11281 agtgtctcca aataaaacaa ttgaagggag tctaggaggg attgcttcag ccattgtggt
    11341 agctttcttt tttatgcttt ttgataagac cgtctatgcg cctcatagct tcctagtcat
    11401 gttagtttta gtggctatct ttagcatttt tggtcaattt ggagatttag ttgaaagtag
    11461 tattaaacgt cattttggtg ttaaagattc gggtaaattg attccgggtc atggggggat
    11521 tttggatcgc tttgatagta tgatttttgt tttcccaatc atgcacttct ttggcttatt
    11581 ctaaaataat atattgtatt tagagaggaa ttgaatgcta ggaattttaa cctttattat
    11641 tatttttggt gtcattgtag ttgttcacga atttgggcat ttctattttg caaagaaatc
    11701 aggaattctt gttcgtgaat ttgctattgg tatgggtcca aaaatctttt ctcacattga
    11761 taaagaagga acaacctata ctattcgtat attaccgcta ggtggttatg tccgtatggc
    11821 tggttggggt gatgataaga ctgaaattaa gacagggacg ccggctagcc ttactttaaa
    11881 taaagaaggc attgtaactc gtattaattt atcaggaaaa caattagaca atacttcttt
    11941 accaatcaat gtcacagctt atgatctaga agataaacta actatcacag gattggtctt
    12001 aagtgagaca aaaacctata gtgttgatca cgatgctact atcattgagg aggatggtac
    12061 tgaaattcgt attgctccct tagatgtgca gtatcagaat gcttctgtat gggggcgctt
    12121 aattaccaat tttgcaggtc cgatgaataa ttttatttta ggtcttgtag tctttattgc
    12181 acttgctttt attcaaggag gtgttcaaga tttatcaacc aatcaggtcc gtgtatcaga
    12241 aaatggtcct gctgctagtg caggtttaaa aaataatgat cgtattttac aaatagggtc
    12301 gcacaaagtt tctaactggg aacagttgac agcagcagta gaaaagtcaa ctaggcattt
    12361 agaaaaaaaa caaaaacttg cccttaagat taagagcaaa gaggtggtga aaacgatcaa
    12421 cgttaagcca cagaaagttg ataaatccta catcatcggc attatgccag ctctaaagac
    12481 aagctttaaa gacaaacttt taggtggatt taaactagca tgggaaagtt tctttagaat
    12541 tttaaatgaa ttaaagaaac ttattgctca ttttagtatt aataaattgg gaggtccagt
    12601 agctctctat caggctagta gccaagctgc taaaaatggt ttcgtcacag ttctcaactt
    12661 aatgggatta atctctatca acttaggtat tatgaatctc atccctatac cagcccttga
    12721 cgggggtaag atagtgatga atattttaga agccattcgt cgcaaaccac ttaaacaaga
    12781 aacagaaact tatattacgc tcgctggggt agcagttatg ttagtattaa tgattgctgt
    12841 aacctggaac gatatcatgc gagctttctt ctaaaagata ataaaacgtg aaaggaaata
    12901 cgaatttttc aattgcaatt ctcaattgaa aaagaatcaa ctttgattag tcgtatctta
    12961 ttgtaatgaa acaaagtaaa atgcttattc caacacttcg cgaaatgcca agtgatgctc
    13021 aggttatcag ccatgctctt atggttcgtg caggatatgt ccgccaagtt tcggctggaa
    13081 tctatgctta tttgcctcta gctaaccgta ctattgaaaa atttaaaaca attatgcgtc
    13141 aagaatttga gaagattggt gcagttgaaa tgttagctcc agctcttctt acagcagatt
    13201 tatggcgtga atctgggcgc tatgaaacat atggtgaaga cctttataaa ttaaagaatc
    13261 gtgatcaatc ggattttatc ttaggaccaa ctcatgaaga gactttcaca actcttgttc
    13321 gtgacgcagt aaaatcttat aagcagttac cactaaacct ttatcaaatt cagtctaaat
    13381 atcgtgatga gaagcgtcct cgtaatggac ttcttcgaac acgtgaattt atcatgaaag
    13441 atggatacag cttccacaaa gattatgagg accttgatgt cacttacgaa gattaccgta
    13501 aagcatacga agccatcttc acacgtgctg gacttgactt taaaggtatt ataggtgacg
    13561 gaggtgctat gggaggtaaa gatagccaag aatttatggc agtaactccc aatcgtacag
    13621 atttaaatcg ttggttagtc cttgataaaa ctattccttc tattgatgat attccagagg
    13681 atgttttaga agaaattaaa gcagaactct cggcttggct agtttctggc gaggacacta
    13741 ttgcatactc cactgaatca agctatgctg ctaaccttga gatggcaaca aatgagtaca
    13801 aaccatctac taaggctgct acctttgaag aagtaacaaa agtagaaact cctaactgca
    13861 aatcaattga tgaggtagct ggatttttat caattgatga aaatcaaact atcaagacac
    13921 tattgtttat tgctgatgaa cagcccgtgg ttgctttact agttggtaac gatcaagtca
    13981 atgatgtgaa gttaaaaaat tatttagcgg ctgatttctt agagcctgca agtgaagagc
    14041 aagcaaaaga gatttttggt gcaggttttg gttctcttgg gccagttaac ctacctgagt
    14101 ctgttaaaat tattgctgat cgtaaagttc aagaccttgc taatgctgtg tctggtgcta
    14161 accaagatgg ttatcatttt actggagtta atccagaacg tgatttcaca gctgaatacg
    14221 ttgatattcg tgaagtaaaa gaaggggaga tttctccaga cggtaaaggt actctgaaat
    14281 ttgctcgtgg tattgaaatt ggacatatct ttaaactagg tacccgttat tcagatagta
    14341 tgggagcgaa catccttgat gaaaatggcc gctcaaatcc tattgttatg gggtgttatg
    14401 gtattggtgt tagtcgtatt ctctcagctg tcattgaaca acatgcccga ctttttgtga
    14461 ataagacacc aaaaggagct tatcgctttg cttgggggat taatttccca gaagaattag
    14521 ccccatttga tgtacatttg attactgtca atgtcaaaga tcaagagtca caagacctta
    14581 ctgagaaaat tgaagctgat ttgatgctta aaggttatga agtcctgaca gatgatcgta
    14641 atgaacgtgt tggttctaaa ttctcagaca gtgacttaat tggacttcca attcgagtaa
    14701 ctgttgggaa gaaagcttct gaaggtatcg ttgaagtgaa aattaaagca agtggtgata
    14761 cgattgaggt tcacgctgat aacttaattg aaactttaga gattctaaca aaaaaataag
    14821 ccgataaaaa agttagtctg atgtgctaac ttttttatat ggagtgaaat ttagtatggt
    14881 tcttattggt aaaaagcaac tgccttttgt tataatgaaa tgatatttta gaaaggagcc
    14941 atcatgaaaa gtcgaaaaaa agataaattg gtattgaggt taacaacaac actattagtt
    15001 tttggtttgg gtggggtttg gttttacaat tataaaaatg ataatgtcga accgacagtc
    15061 actagtgcat cggatcaaac gacgactttt attcaaacga tttccccaac agctattgaa
    15121 atttctaaga cctatgattt gtatgcgtca gtcttattag cacaagctat tttggaatca
    15181 tccagtggac aatcagattt gtctaaggct cctaattata acctctttgg catcaaagga
    15241 gaatataaag gtaaatctgt tcaaatgcct actttagaag atgatgggaa aggtaatatg
    15301 acccaaatcc aagctccttt tcgcgcctat ccaaattatt ctgcttcact atatgattat
    15361 gctgagttag tatctagtca aaagtatgca tctgtttgga aatcaaatac ctcttcttat
    15421 aaggatgcta ctgcagctct aacaggtctt tatgcgacag atactgctta tgctagtaaa
    15481 ttaaaccaaa ttattgaaaa ctacagtcta gatgcttatg ataaatagtt tcagttaaga
    15541 gagtgttgtt aagaaggcta ataaatgcct tttttaacag cctccttttg ttatctcgaa
    15601 atatttacag atatgtgata aaatggaagt aagatattca ggagaaaact atgtcagaat
    15661 tgttcaagaa acttatggat cagattgaaa tgccgcttga aattaagaat tcaagtgttt
    15721 tttcgtctgc tgatattatc gaggtaaagg ttcattctct atcacgctta tgggaatttc
    15781 atttttcatt tccagaatta ttgccaattg aggtgtatcg tgaattacaa actcgtttag
    15841 tgaatagttt tgaaaaagca gatataaagg ctacttttga tataagagca gaaactattg
    15901 atttttctga tgatctgcta caggattatt atcaacaagc tttttgtgaa cccttgtgta
    15961 atagtgcaag ttttaagagt tctttttcac aattaaaagt tcattacaat ggcagtcaga
    16021 tgattatttc tgcacctcaa tttgtcaata ataatcattt tcgtcagaat catttgccgc
    16081 gtttggaaca acagttttca ttgtttggat ttggtaaact tgctattgat atggtttctg
    16141 atgaacaaat gacgcaagac ttaaaatcat catttgaaac taatcgcgag cgattgcttg
    16201 aaaaagctaa ccaagaagct atgcaagcct tggaaacaca aaaatcacta gaagattcag
    16261 cgcctccatc agaagaagtg accccaactc aaaattatga tttcaaagag cgtatcaaac
    16321 aacgtcaagc tggctttgaa aaagcagaga ttacacccat gattgaggtg acaacagaag
    16381 aaaatcgtat cgtttttgaa ggaatggttt ttagtgttga acgaaaaaca acccgcactg
    16441 gacgtcatat cattaatttt aagatgactg attatacctc aagttttgct atgcaaaagt
    16501 gggctaaaga tgatgaagaa ttgaagaagt acgatatgat ttctaaaggt tcgtggttgc
    16561 gagtcagagg gaatattgag aacaataact ttactaaatc tttgacaatg aatgtccaag
    16621 atatcaaaga gattgtgcat catgaacgta aagacttaat gccagcagat caaaaacgtg
    16681 tggaatttca tgctcatacc aatatgtcaa caatggatgc tttaccaact gtcgagagcc
    16741 tcattgatac tgcagctaag tggggacacc cagctattgc aattaccgac catgccaatg
    16801 ttcaaagttt tccgcacggc taccacagag caaaaaaagc aggtataaaa gcaatttttg
    16861 gattagaggc aaatattgtt gaagataaag tgcctatttc ctacaacgaa gttgatatga
    16921 atttgcacga agcaacttat gttgtatttg atgtggaaac tacaggtctt tctgcagcta
    16981 ataatgacct aattcagatt gcagcatcaa aaatgttcaa gggtaatatt attgagcaat
    17041 ttgatgagtt tattgaccca ggtcatcctc tttctgcctt tacaactgag ttgacaggta
    17101 ttacggataa tcatgtcaga ggttcaaaac ctattttaca agtccttcag gaatttcaga
    17161 atttttgtca aggtacggtt ttagttgcgc ataatgcaac ctttgacgtt ggttttatga
    17221 atgcgaatta tgaacgtcat aatctgcctt tgatcacaca gccggtcatt gataccttag
    17281 aatttgcaag aaatctttat cctgaatata agcgtcatgg tttggggcct ctaaccaaac
    17341 gtttccaagt tgctttggaa catcatcata tggccaacta cgatgctgaa gcaacgggtc
    17401 gcttactctt tattttctta aaagaggcgc gggaaaatcg tgatgtgact aatttgatgg
    17461 agctcaatac taaattggta gctgaagact cttacaaaaa ggcacgcata aagcatgcaa
    17521 ccatttatgt ccaaaatcag gttggactta aaaatatatt taagttggtt agcctttcaa
    17581 atgttaaata ttttgaaggg gtggcaagaa ttcctaggtc agttttagat gctcacagag
    17641 aaggtctctt gctaggtact gcctgttctg atggagaagt ttttgatgcc ttgttatcaa
    17701 atggaattga tgcagcagtg accctagcta aatattatga ttttattgaa gtcatgccac
    17761 ctgctattta tcgccccttg gtggttcgtg atttaattaa agatgaggcg gggattcaac
    17821 aaattattag agacttaata gaggtcggtc ggcgtttaga taagcctgtt ttagctacgg
    17881 gaaatgttca ttatattgag cctgaagatg agatttaccg tgagattatt gttcgtagtc
    17941 ttggacaagg tgccatgatt aataggacta tcggtcgtgg ggaggatgct caaccagcgc
    18001 ctttgccaaa agcacatttc cgcacaacaa atgagatgtt agatgaattt gcctttcttg
    18061 gtaaagattt agcctatgag atcgttgtaa ccaatacgaa tacttttgca gatcgatttg
    18121 aagacgttga agttgtaaaa ggggatctct atactccgtt tgttgatcgt gctgaagaac
    18181 gagttgctga attaacttat gctaaagcat tcgaaatata tggtaatcct cttcctgata
    18241 ttattgattt acgtatcgaa aaagagttag catctatact tggtaatgga tttgccgtta
    18301 tctatcttgc ttcgcaaatg ttggtgcaac gttctaatga acgtggttat ctagtaggat
    18361 cacgtgggtc tgttgggtcg agttttgtag cgaccatgat aggaattact gaagttaatc
    18421 caatgccacc tcattatgtt tgcccaaatt gtcagcattc tgaatttatc acagatggtt
    18481 cttgtggttc aggctacgat ttaccaaata aaaattgtcc taaatgtggt actttgtaca
    18541 agaaagatgg acaggatatt ccgtttgaaa ctttcttagg atttgatgga gataaggttc
    18601 ctgatattga tctgaatttc tctggagatg atcaaccttc tgctcactta gatgttcgtg
    18661 atatctttgg agaagaatat gcttttcgtg caggaacggt aggtaccgta gctgaaaaaa
    18721 ctgcttttgg ttttgtcaaa ggttacgaac gagattacaa taaattttat aacgatgcag
    18781 aggtcgaacg gttagcgact ggtgctgcgg gagttaaacg ttctacaggt caacaccccg
    18841 gtggtattgt tgttattcct aactatatgg atgtttatga ttttactccg gttcaatatc
    18901 cagcagatga tatgacagct gcttggcaga caactcactt caatttccat gatattgatg
    18961 agaatgtctt aaagttagat attctaggac atgatgatcc tactatgatt cgtaaacttc
    19021 aggatttatc aggaattgat ccatcaaata tccttccaga tgatccagac gttatgaagt
    19081 tattttctgg tacggaagtt ttaggagtga cagaagagca aattggaact cctacaggaa
    19141 tgcttggtat tccagagttt ggtacgaact ttgttcgtgg tatggtgaac gaaacccatc
    19201 caaccacatt tgcagaattg ttgcaactgt caggactatc acatggtacg gatgtttggt
    19261 taggtaatgc tcaagactta atcaaagaag gtattgctac cttatcaacc gttattggtt
    19321 gtcgtgatga catcatggtt tatttaatgc acgctggtct acaaccaaag atggccttta
    19381 caatcatgga gcgcgtgcgt aaggggttat ggttgaaaat ttcagaagat gagcgtaacg
    19441 gttatatcca agcaatgcgt gataataatg tcccggattg gtatattgaa tcgtgtggaa
    19501 aaatcaagta tatgttccct aaagcccatg cagcagccta tgttttaatg gcattacgtg
    19561 tggcgtactt taaagtacat tatccaatat tttattactg tgcttatttt tctattcgcg
    19621 ctaaagcttt tgaattacgc acaatgagtg ctgggcttga tgccgttaag gcgcgcatga
    19681 aggatatcac tgagaagcgt cagcgtaatg aggcgacgaa tgtcgaaaat gaccttttta
    19741 cgacattaga gttggttaat gaaatgcttg agcgtggctt taaatttggg aaattagatc
    19801 tttatcgtag ccatgcgact gactttatta ttgaagagga tacgctaatc cctccatttg
    19861 ttgctatgga aggcttggga gaaaacgttg caaaacagat agttcgggca cgtgaagatg
    19921 gtgaattttt atcaaaaaca gagcttcgta agcgaggagg agtctcgagt accttagttg
    19981 aaaaatttga tgagatgggt attttgggaa atttaccaga agacaatcag ttgagtcttt
    20041 ttgatgattt cttctaagca ataaggttga gtagttactc aaccttatta tttgccagct
    20101 gcttttgata gggcaaagat gatattcttg cttgaaataa ctaattagtg ataaaataag
    20161 aaaaatacgt ttgggagatg aaatgggaaa ctttaaaaat tcagcggtta aatctatggt
    20221 tgtcatgcga aaagcattta gaacaataga cggaaaagtc tcagaatcat ttaaagaatt
    20281 tgaattaaca ccaactcagt ttgcagtttt ggatgttctc tatgctaaag gcacaatgaa
    20341 aattggagaa ttgattgaaa atatgcttgc tacatcaggt aatatgactg ttgttattaa
    20401 aaatatggaa aaaaaaggat gggtattacg ccattcttgt ccgaatgata agagagcctt
    20461 tttggtgagt ttaacaactg aaggagaaga agtgataaaa aaagctctac cagagcatat
    20521 taaacgtgta gaagatgctt tttcagtctt aacagaaaca gagcaagaag acttgattaa
    20581 ccttttaaaa aaatttaaaa ccttgtgagt cacattcttt gactttacga tatagtggta
    20641 atataattac tacatagtaa taaataactt taatctaatt catatgtcac tagaacaaat
    20701 gtagtaagat atctgagatt gaagaacaag gagaaaaaat atgtcatttt tggaagaatt
    20761 aaaaaaccgt cgctctattt atgctcttgg tcgcaataca gaagtttcta atgagaaaat
    20821 tgtagagatt attaaagagg cagttcgcca atccccatct gcttttaata gtcaaacttc
    20881 ccgtgttgtc attttattga atgatgaagt tactaagttc tgggatgaat tggttgctaa
    20941 tgatttggta gaaacaatga aagtacaagg tgctccagag acagctattg ctggaactaa
    21001 ggaaaaatta gctagttttg gagcttcaaa gggaacagta ctcttctttg aagatcaaga
    21061 tgttgtcaaa tctcttcaag aacaattcgt tctttatgcg gataactttc cagtttggtc
    21121 tgagcaatca acaggtattg cctcagtaaa tacttggaca gccctttctg cagaacttgg
    21181 acttggtggt aatttacaac attacaatcc agttattgat gcatcagtac aagctgttta
    21241 tggtgttcca gctagttgga aacttcgtgg acaactaaat tttggttcaa tcgaagctga
    21301 aacaggagaa aaagaattca tgaatgatga tgaccgcttt aaagtcatcg gataatactg
    21361 tacaaaaaag acaaggatag caaccttgtc tttttattgt gttaattcag atttagctgt
    21421 attttgtatt gtttagccat ttctgtgatt ttatcaaatt cccccaaagc tgctagttta
    21481 ttgaactctc caccaatacc aatggctgtt actccagata gaaaccagtc tttggcattt
    21541 gtgagattta ccccaccagt caccataatt tgtacttgag gtaaaggtgc ttttagtgat
    21601 gagataaaag atgttcctag agtacctcct gggaatattt taattatttc acaaccagct
    21661 tcaagtgctg ttgttatctc tgataaggtc atgcaacctg gtaaataagg aatagcataa
    21721 cgattacata gtttagcagt ctgtagatta aatgcgggag atacaataaa ctttgctcct
    21781 gctaaaatag ccattcgggc agtttctgag tctagaacag ttcctgcccc aacgacaact
    21841 tgttcttggt tagtatattg tgtgactagt tgttcaatga cttggctagc tttgctatta
    21901 gtataggcga tttcgatagc agaaatacca ccttttatgc atgcttgagc agcttgaaaa
    21961 gcttcttctt ggctatttcc acgaatgaca gcaactattt ttccttgcat taattgcaat
    22021 aaaatctttg attttatcat aattctccct tttaacgaat aatgtctttc gattgattta
    22081 gtactttgtt aactgcggca acggtctcaa acatattgtc cccttccaaa gcacatttta
    22141 aagatgcact agcgacagcg aaatcaatga ctgtcttgct gtctgagtgc tgaagtagtt
    22201 gatatagcgc tcccgccaca aaagcatctc cgcttccaat tctctggttt acagctgtgg
    22261 tgatttcttt tgaagtgaca aactcttgtt tacgatttgc tatgtaagct tggtagtggt
    22321 ttcgtcccca ttcatcctgt agacgtttag tatggaaaat gacttgaaag tcaaagtgat
    22381 ttataagaga aatcattcgt tctttgatgt cctctattgt agcttcatcg cgattgaaga
    22441 aggtagtgtc ctgtgagtca accataagag gttcaatacc aaaacaaata tccgcaaagg
    22501 tagcgaactg agaaaaaagg attttggcat ttttaggaga aattagttta ctgcgaaaat
    22561 tgagatctaa ggagattgtt atctctcttt ttttagcttc ttttaaaaga agcagtgtta
    22621 tctcttggat agactcatcc aatgatagtg taataccact aaaatgaaag agagaaactc
    22681 cttcaaataa ttgatcgaaa tcaatttggt ttatcctaaa gtcatgtaaa ctactataat
    22741 cgcggtcgta gaccacctca ccttttctgc aagcaaagga attttctaag aaatataacc
    22801 caacacgttc tccaacccac tgaatagaat gagtatcaat accctgtgct tttaaaaatt
    22861 gaagaaaact attacctatg ggattattgg gtaaggctga gaggagttta gtatcttgcc
    22921 caaagccttg tagagctctt gcgatattaa cttcagagcc accatagaat agtttagttg
    22981 agatactatc tgcaaaatag tcattttctt ttggagtgat gcgaatgaga ggttcaccga
    23041 aaaaaagaat cttagacata aaagtccttt ctaaaacaga cgctttagtc taagacagat
    23101 ttcaagtagt ctgctatatt ttgattttgg caattagcga agaaatattc ttggaaatgc
    23161 tccccagaaa tggtttcctt taagaaatct tgatcaattt cttttaaaac agagagtaaa
    23221 tctctgtaag taatctgttt tacttcgcta aggatacgtg cattgcgttg ctcaggcaca
    23281 gccctctctt tagggtatcc ccctcctttt tcatcttcga aaagacgttc aaataaatac
    23341 ctcagattca attcagctcc ccacccaaat cctttagcaa aaggaagaga aagagcattt
    23401 cctccgttta cttgagagaa taagtaagcg tcgacaggat cagcagcgaa tccacagact
    23461 acgccaggaa aactattaca agcaagcatc gctccaattc ctgtcccaca gcctgtaata
    23521 ataaaatctg cagcaccgct attaagtaaa atagcagtca gtaaaccatt ttgtacataa
    23581 gttagctggc tttcttcttc ttctttgcca tacatcccgt agttgaaaac atcaaatcct
    23641 tttttgtcag aaaccgcttt taattctttg tagataatag tatttttact tgcttggcta
    23701 ttttcgttga ttaatgcaat tttcatttat attactcctt tattattgag gttgtttacc
    23761 gatgtaagct aaaataccgc catctacata gagtatgtga ccattgataa agtttgatgc
    23821 atcgctagca aggaagatag ctggtgcacc taaatcttct gcctcacccc agcgagcggc
    23881 aggtgtttta gcgatgataa attggtcgaa tggatgacgt gaaccatcat cttgacgttc
    23941 acgaagggga gctgtttgtg gtgtggcaat atagccgggc ccgataccgt tacattggat
    24001 gttagcagaa ccgtattcag aagcgatgtt cttggttaac attttaagtc cccctttggc
    24061 agcagcatag gcagcaactg tttcgcgtcc aagctcactc atcatggagc aaatattgat
    24121 gattttacca tgtccttttt ggatcattcc aggtaatacc gcttttgata cgataaaggg
    24181 agcattcaaa tcaatatcaa taacttgtcg aaaatctgca gcgctcatct ctagcatagg
    24241 ggtgcgtttt atgatacctg cgttgttaac tagaatatca attaccccaa catcttgaga
    24301 aattttgttc accatttcgt tgattccatc ttcatctgta acgtcacaaa catagccatg
    24361 ggccttaatg ccaagttttt tataggcgga gatgcctttg tcaacgagtt cctgcttaat
    24421 atcattgaag acaatagttg ctccagctct agcaaaagca gttgctattg aaaaaccaat
    24481 gccgtaagaa gcacctgtta ttaaggcgac tttaccttct agtgaaaagt tgtcttttaa
    24541 gaattgttca gtcataatat gacctccgta aaaaaattca ggaagtaaac ctgtttacat
    24601 gaatagtaac aaagtaaacc ggttttgtca actatagagt tcttcttcta taaaacttgg
    24661 caacatatat aataaagatt tatctaggtt ataattctta aagataaaaa agacatcatt
    24721 ataaaaaaat caagtaaact gctcaaaaaa tataaaaaag atgttgacac ctaaaaatga
    24781 aagcgctata atctgattat aaaagtaaac cggtaaactc aagaggagaa aatatgataa
    24841 aaattattat tgtagcacac ggcaactttc cagatggtat tttaagctct ttagaactta
    24901 ttgctgggca tcaggaatat gttgctggta ttaactttat tgcaggcatg tcttctaatg
    24961 atgttagagt tgctttgcaa agagaggtta tcgatttcaa agaaatttta gtattgactg
    25021 atttgctagg tgggacccct ttcaatgttt cttccgcctt atcagtagaa tatacagata
    25081 aaaagataaa agttttatcg ggcttgaacc tttcaatgtt aatggaagct gttttatcta
    25141 gaacgatgtt tgaacatgta gatgaccttg ttgataaagt tataacatca tctcgtgaag
    25201 gaattgttga tttttctaat tgccttgcta cacaaattgc agaagtaact tttgaaggag
    25261 gcatttaata atgatgaaaa taaaaccggt caaggttgag tcaattgaaa atccaaagcg
    25321 ttttttagac ggtagattat taactaagat tgaagttgag ggagcgattg aaaaagcctt
    25381 gaagcaactt tatattaata ttgattactt tggtgaagag tatccaacac ctgcaacatt
    25441 caataatact tataaagtta tggataacac agaatggaca aatggttttt ggacagggtg
    25501 cttgtggtta gcttatgagt ataatcagga taaaaagtta aaaaacatag cccataaaaa
    25561 tgtattgtca tttctaaatc gtattaataa tcgtatagca ttagatcacc acgacttagg
    25621 atttctttac acaccatctt gtacagcaga atatcgtatc aatggtgatg ttaaagcttt
    25681 agaagccact ataaaagctg cagataaatt gatggagcgc tatcaagaaa aaggtggatt
    25741 tattcaggct tggggagaac tcgggtataa ggaacactat cgcttaatta tcgattgctt
    25801 acttaatatc caactcttat tttttgctta tgaacagaca ggtgatgaaa agtatagaca
    25861 agttgcggtg aatcacttct acgcttcagc taacaatgtg gtgcgtgatg attcttctgc
    25921 ttttcatact ttttatttcg acccagaaac tggagaaccg ttaaaaggtg tcacacgaca
    25981 gggttatagt gatgagtcat cttgggcaag agggcaagca tggggcatct acggtattcc
    26041 gcttagttac cggaaaatga aagattatca gcagattatc ctttttaaag gtatgacaaa
    26101 ctattttcta aatcgtttac ctgaagacaa ggtatcctat tgggacctta tttttacgga
    26161 tggctcaggt cagcctagag atacatccgc aacagcaacg gctgtgtgtg gaattcatga
    26221 gatgcttaaa tatttaccag aagtagatcc tgataaagag acatacaaat atgctatgca
    26281 tacaatgctt cgtagtctaa ttgaacagta tagtaataat gaacttatag caggacgtcc
    26341 tcttctattg cacggtgtgt attcgtggca ttcaggtaaa ggagtagatg aaggtaatat
    26401 ttggggagat tattattact tagaagcctt aataagattc tataaagact gggaacttta
    26461 ttggtaagag tattttacta acaaaataaa caataaataa tagaaaacga ggaggtcaga
    26521 aatggcagca ggaccaaata ttgtaatgac acgtgttgat gagcgtctta ttcatggaca
    26581 aggacaactt tgggtgaaat ttttaagttg taatactgtt attgtggcaa atgatgatgt
    26641 ctccaaagat catcttcaac aaacgttaat gaagacggtg gtgccagaat caatagcatt
    26701 gcgatttttt gatattcaaa aagttattga tattattcac aaggccaacc cagcacagac
    26761 gatttttata attgt
//
LOCUS       DATRNK010000024        24768 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.024, whole genome shotgun sequence.
ACCESSION   DATRNK010000024 DATRNK010000000
VERSION     DATRNK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24768
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.024"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            49..1014
                     /locus_tag="VBC52_001002"
     CDS             49..1014
                     /locus_tag="VBC52_001002"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEO6882599.1"
                     /translation="MTIKNLTVAGSGVLGSQIAFQAAYKGMSVTIYDINDEALNKGKE
                     RIKKLAKVYQSEIETAKEAYSDKAKSIKYNKNLLPSLDHIFLSKVADSLDLIADLPNQ
                     ITFSKNLDQAVSDADLVIEAVPETVSIKEDFYKQLAKVAPSKTIFATNSSTLVPSQFA
                     DITGRPDKFLAMHFANNIWQNNIVEIMGHKGTDDEVIKEALAFSKDIGMVPLHIHKEQ
                     PGYILNSILVPFLESALALYYDKVSDSETIDKTWKLGTGAPMGPLEILDIIGIDTAYN
                     IMKNYSDTNSDPNSLHAHLAKMLKEEFIDKGRTGKAAGHGFYDYD"
     gene            complement(1061..1609)
                     /locus_tag="VBC52_001003"
     CDS             complement(1061..1609)
                     /locus_tag="VBC52_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO6882600.1"
                     /translation="MTKALISIDYTYDFVADDGKLTAGKPAQSIASAIADVTEKAYRS
                     GDYIFFAIDNHDIGDVFHPESNLFPEHNIKGTSGRNLYGPLGTLYETIKEDSRVFWID
                     KRHYSAFSGTDLDIRLRERRVDTLILTGVLTDICVLHTAIDAYNLGYKIEVPAAAVAS
                     LNDSNHQWALNHFKTVLGATIL"
     gene            complement(1676..2461)
                     /gene="codY"
                     /locus_tag="VBC52_001004"
     CDS             complement(1676..2461)
                     /gene="codY"
                     /locus_tag="VBC52_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEO6882601.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLDAELPYNTMAAQLADIIDCNAC
                     IINGGGNLLGYAMKYKTNTDRVEEFFETKQFPDYYVKSASRVYDTEANLSVDNDLSIF
                     PVETKENFQDGITTIAPIYGGGMRLGTFIIWRNDKEFSDDDLILVEIASTVVGIQLLN
                     LQTENLEENIRKQTAVTMAINTLSYSEMKAVAAILGELDGLEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFDKLKEYN"
     gene            complement(2587..3798)
                     /locus_tag="VBC52_001005"
     CDS             complement(2587..3798)
                     /locus_tag="VBC52_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6882602.1"
                     /translation="MKIFDKSMKLEHVAYDIRGPVLEEADRMRANGEKILRLNTGNPA
                     AFGFEAPDEVIRDLITNARESEGYSDSKGIFSARKAVMQYYQLQNIHVDMDDIYIVNG
                     VSEGISMSMQALLDNDDEVLVPMPDYPLWTACVSLAGGNAVHYICDEEANWYPDIDDI
                     KSKITSKTKAIVLINPNNPTGAVYPREILQEIVDIARQNDLIIFSDEVYDRLVMDGME
                     HIPIASIAEDIFTVTLSGLSKSHRICGFRVGWMVLSGPRQHVKGYIEGLNMLANMRLC
                     SNVLAQQVIQTSLGGQQSIDSMLLPGGRIYEQRNYIHKAINEIPGLSAVKPNAGLYLF
                     PKIDTDMYRIDNDEEFVLNFLKQEKVLLTHGRGFNMNTADHFRIVYLPRVDELTELQE
                     KMARFLSRYKR"
     gene            4065..4517
                     /locus_tag="VBC52_001006"
     CDS             4065..4517
                     /locus_tag="VBC52_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO6882603.1"
                     /translation="MSQKYERILVAIDGSTESELAFEKAVNVALRNDSELILTHVIDT
                     RALQSFATFDTYIYEKLEKEAKDVLEEYEKQAREKGADKVRQVIEFGNPKTLLAHDIP
                     EKEKVDLIMVGATGLNTFERFLIGSSSEYILRHAKVDLLIVRDPNKTM"
     gene            complement(4573..5955)
                     /locus_tag="VBC52_001007"
     CDS             complement(4573..5955)
                     /locus_tag="VBC52_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO6882604.1"
                     /translation="MAIKAVFFDIDGTLLNDRKNVQKSTIKAIRNLKDQGILVGLATG
                     RGPSFVQPFLENLGLDFAVTYNGQYIYSRSEIIYTNQLSKTTVYRLIRYAGARRREIS
                     LGTASGLLGSGIIGLGTSRLGQIVSSLVPRKWAKAIERSFKHFIRRIKPQNIDSLMVI
                     LREPIYQVVLVATEGESERIQKQFPRVKLTRSSPYSMDVISEGQSKVKGIERVGQRYG
                     FDLSEVIAFGDSDNDIEMLSQVGIGVAMGNASQQVRENARYTTADNNDDGISKALAHY
                     GLIQFEIEKTFSSRDENFNKVKSFHLLMDGETIETPRLYDSKEAGFRSDFKVEEIVEF
                     LYAASQGNQKVFDQSIRNLHLAIDKARDKVISKDHPETPLVGEVDALTDLLYLTYGSF
                     VLMGVDPKPLFDTVHEANMGKIFPDGKAHFDPVTHKILKPDDWEEHFAPEPSIRRELD
                     SQIQKSLNRK"
     gene            6024..6986
                     /locus_tag="VBC52_001008"
     CDS             6024..6986
                     /locus_tag="VBC52_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEO6882605.1"
                     /translation="MKKILVLHTGGTISMNANEKGQVMSSADNPMKYVDLSLDDLDLT
                     VVDFLNLPSPQITPHHMLDIYHYLKQHASNFDGVVITHGTDTLEETAYFLDTMILPKI
                     PIIITGAMRSTNELGSDGVYNYLSALRVANSTKAADKGVLVVMNDEIHAAKYVTKTHT
                     TNVSTFQTPTHGPLGIIMKQDLLFFKATKERVRFDLDKITGTVPIVKAYAGMGDSGII
                     SFLNSQNISGLVIEALGAGNMPPKAAQEIEELIEQGVPVVLVSRCFNGIAEPVYGYEG
                     GGAKLQESGVMFVKELNAPKARLKLLIALNAGLTGQNLKDYIEG"
     gene            complement(7021..7899)
                     /gene="aroE"
                     /locus_tag="VBC52_001009"
     CDS             complement(7021..7899)
                     /gene="aroE"
                     /locus_tag="VBC52_001009"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEO6882606.1"
                     /translation="MNSLNGETLLTGLIANPARHSLSPLMWNTSFQEKNMNYAYLTFE
                     VEEGKLTEAVRGVRALGIRGVNVSMPFKQSVIPLLDDLSPQAKLVGAVNTIVNQGGTG
                     RLVGHMTDGIGCFKALAAQGFSAKNKIITIAGIGGSGKAVAVQAAMEGVAEIRLFNRN
                     SSNYDKVIDLSDKIKKQFQIKVVVDYLENKTAFKDAIRTSHFYIDATSLGMRPLDNYS
                     LINDPEILTPNLVVVDLVYKPKETALLRFVRQNGVKHAYNGLGMLIYQGAEAFQLITN
                     QEMPISSVERVLQTEN"
     gene            complement(7997..8911)
                     /locus_tag="VBC52_001010"
     CDS             complement(7997..8911)
                     /locus_tag="VBC52_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6882607.1"
                     /translation="MLQNIGQTGIQATRIALGCMRMSDLKGKQAEEVVGTALDLGINF
                     FDHADIYGGGLSELRFRDAIKHLNVNRDKMIIQSKCGIREGYFDFSKEYILSSVDGIL
                     ERLGTEYLDFLILHRPDVLVEPEEVAEAFTKLRAEGKVKHFGVSNQNRFQMELLQSYL
                     DEPLAVNQLQLSPAHTPMFDAGLNVNMLNKASIEHDDGIVDYCRLKRVTIQAWSPFQI
                     DLSRGLFVNHPDYKELNETIAKLAKNYNVSSEAIVIAWILRHPAKMQAIVGSMNPSRL
                     KAIDKANDIALTRKEWYDIYRSAGNILP"
     gene            complement(9202..11217)
                     /gene="recG"
                     /locus_tag="VBC52_001011"
     CDS             complement(9202..11217)
                     /gene="recG"
                     /locus_tag="VBC52_001011"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEO6882608.1"
                     /translation="MLLQSPISNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVAQGISQSTLIKAIKSAFE
                     ISAHLELKENLPATLLEKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKSENKSETNGLPILYSKHAMETKISSLPFILTNAQKRSLDEILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSIIDELPAGRKPIITRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     YKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARRVASDIVKDNNWKENTEWALILDNLRQHSDFD"
     gene            complement(11297..12835)
                     /locus_tag="VBC52_001012"
     CDS             complement(11297..12835)
                     /locus_tag="VBC52_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6882609.1"
                     /translation="MSKQKVMATLLLSTLVLSLSSPLVTLAETINPETSLTMATASTE
                     SSSEAEKQEKTQPTDSETASPSAEGSISTEKTEIGTTETSSSNESSSSSSHQSSSNED
                     AKTSDSASTASTPSTNTTNSSQADSKPGQSTKTELKPEPTLPLVEPKITPAPSQIESV
                     QTNQNASVPALSFDDNLLSTPISPVTATPFYVEHWSGQDAYSHYLLSHRYGIKAEQLD
                     GYLKSLGIQYDSNRINGAKLLQWEKDSGLDVRAIVAIAVLESSLGTQGVAKMPGANMF
                     GYGAFDHDSSHASAYNDEEAIMLLTKNTIIKNNNSSFEIQDLKAQKLSSGQLNTVTEG
                     GVYYTDNSGTGKRRAQIMEDLDRWIDQHGGTPEIPAALKALSTASLADLPSGFSLSTA
                     VNTASYIASTYPWGECTWYVFNRAKELGYTFDPFMGNGGDWQHKAGFETTHSPKVGYA
                     VSFSPGQAGADGTYGHVAIVEEVKKDGSVLISESNAMGRGIVSYRTFSSAQAAQLTYV
                     IGHK"
     gene            complement(12928..14028)
                     /locus_tag="VBC52_001013"
     CDS             complement(12928..14028)
                     /locus_tag="VBC52_001013"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO6882610.1"
                     /translation="MISSYHRPTRALIDLEAIANNVKSVQEHIPSDKKTFAVVKANAY
                     GHGAVEVSKYIESIVDGFCVSNLDEAIELRQAGIVKMILVLGVVMPEQVILAKNENIT
                     LTVASLEWLRLCQTSAVDLSGLEVHIKVDSGMGRIGVRQLDEGNKLISELGESGASVK
                     GIFTHFATADEADNCKFNQQLTFFKDFISGLDNCPDLVHASNSATSLWHSETIFNAVR
                     LGVVMYGLNPSGTDLDLPYPINPALSLESELVHVKQLHDGSQVGYGATYQVTGDEFVG
                     TVPIGYADGWTRDMQGFSVIVNGELCEIIGRVSMDQMTIRLPQKYTIGTKVTLIGQQG
                     SCNITTTDVAQKRQTINYEVLCLLSDRIPRYY"
     gene            complement(14025..14384)
                     /locus_tag="VBC52_001014"
     CDS             complement(14025..14384)
                     /locus_tag="VBC52_001014"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEO6882611.1"
                     /translation="MIVGHGIDLQEIEAITKAYERNQRFAERVLTEQELLLFKGISNP
                     KRQMSFLTGRWAAKEAYSKALGTGIGKVNFHDIEILSDDKGAPLITKEPFNGKSFVSI
                     SHSGNYAQASVILEEEK"
     gene            complement(14409..15416)
                     /locus_tag="VBC52_001015"
     CDS             complement(14409..15416)
                     /locus_tag="VBC52_001015"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEO6882612.1"
                     /translation="MGFHQLSDKINIEILKQKTSLDLEVSQKKLAKEEELKNIIKGED
                     QRFLVIVGPCSADNPKAVLTYAKRLAKLEAAFKDKMFLVMRVYTAKPRTNGDGYKGLV
                     HHSDKLGVFFQARKMHYDIIRETGLLTADELLYPEMLSVMDDLVSYYAIGARSVEDQG
                     HRFISSGIDAPVGMKNPTSGNLRVMFNAVYAAQNQQELFYQNKQVRTDGNLLSHVILR
                     GYHNADYRSIPNYHYENLLETITHYEETDLQNPFIVVDTNHDNSGKQFLEQIRIVKSV
                     LADRQWHTKIRNYVRGFLIESYLEDGRQDKPDVFGKSITDPCLGWDKTEMLIRDIYNS
                     L"
     gene            complement(15542..18070)
                     /gene="secA"
                     /locus_tag="VBC52_001016"
     CDS             complement(15542..18070)
                     /gene="secA"
                     /locus_tag="VBC52_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEO6882613.1"
                     /translation="MANILRTVIENDKGELKKLDKIAKKVDSYADHMAALSDEALQAK
                     TPEFKERYQNGETLDQLLPEAFAVVREASKRVLGLYPYHVQIMGGIVLHHGDIPEMRT
                     GEGKTLTATMPVYLNAISGLGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PFEKREAYNCDITYSTNAEVGFDYLRDNMVVRQEDMVQRPLNYALVDEVDSVLIDEAR
                     TPLIVSGPVSSEMNQLYTRADMFVKTLNSDDYIIDVPTKTIGLSDTGIDKAENYFHLN
                     NLYDLENVALTHYIDNALRANYIMLLNIDYVVSEEQEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKESVPIQEESKTSASITYQNMFRMYHKLAGMTGTGKTEEEEFREIYNMRVIP
                     IPTNRPVQRIDHSDLLYPTLDSKFRAVVADVKERYEQGQPVLVGTVAVETSDLISRKL
                     VAAGVPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGTDRIKVVLERMNLAE
                     DDTVIKSKMLTRQVESAQRRVEGNNYDTRKQVLQYDDVMREQREIIYANRREVITAER
                     DLGPELKGMIKRTIKRAVDAHSRSDKNTAAEAIVNFARSALLDEEAITVSELRGLKEA
                     EIKELLYERALAVYEQQIAKLKDPEAIIEFQKVLILMVVDNQWTEHIDALDQLRNSVG
                     LRGYAQNNPIVEYQSEGFRMFQDMIGSIEFDVTRTLMKAQIHQQERERASQHATTTAE
                     QNISAQHVPMNNESPEYQGIKRNDKCPCGSGMKFKNCHGLRCLQ"
     gene            complement(18179..19126)
                     /gene="manA"
                     /locus_tag="VBC52_001017"
     CDS             complement(18179..19126)
                     /gene="manA"
                     /locus_tag="VBC52_001017"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEO6882614.1"
                     /translation="MSEPLFLEASMHDKIWGGTKLRDEFGYDIPSETTGEYWAISAHP
                     NGVSRVKNGRFKGCFLDKLYQGEKSLFGNPDDTVFPLLTKILDANDWLSVQVHPDDAY
                     ALKHEGELGKTECWYIISADEGSEIIYGHNAKTKEELRQMIESGDWEHLLTRIPVKSG
                     DFYYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRPDASGKLRDLHIEQSIDVLTI
                     GKPANTVPANMKLKHLSSTLLVSNDFFTVYKWEISGVTNFKQFAPYLLVSVLDGAGHI
                     TVDNKVYTLKKGDHFILPNDVVKWDIDGQLEIIASHVKK"
     gene            complement(19244..20125)
                     /locus_tag="VBC52_001018"
     CDS             complement(19244..20125)
                     /locus_tag="VBC52_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO6882615.1"
                     /translation="MTKLYGSIEAGGTKFVCAVGDEELKVVEKMQFPTTTPQETIKKT
                     VDFFKRFEKKLEAVAIGSFGPIDIDKKSKTYGYITTTPKLHWANVDLLGLISKDFNVP
                     FYFTTDVNSSAYGEVIARNNIDSLVYYTIGTGIGAGAIQKGEFIGGTGHTEAGHTYMA
                     MHPQDQANDFKGICPFHNSCLEGLASGPTLEARTGIRGELIEENSMVWDVQAYYIAQA
                     AMQATVLYRPQVIVFGGGVMAQEHMLRRVRQTFATLLNGYLPVPDLSDYIVTPAIEEN
                     GSATLGNFALAKKISKG"
     gene            complement(20193..22112)
                     /locus_tag="VBC52_001019"
     CDS             complement(20193..22112)
                     /locus_tag="VBC52_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEO6882616.1"
                     /translation="MASNTEIAKQVINAIGGASNVRSVAHCATRLRVMVKDETVIDKN
                     TVENIEKVQGAFFNSGQYQIIFGTGTVNKIYDEVVAQGLPTSSTSDQKAEAAKQGNAF
                     QRAIRTFGDVFVPLLPAIVATGLFMGIRGAINNDTVLALFGTTSKAFSSSNFYTYTVV
                     LTDTAFAFFPALISWSAFRVFGGNPVIGLVLGLMMVNSALPNAWAVASGDAHPIKFFG
                     FIPVVGYQNSVLPAFFVGLLGAKLEKWLHKKIPDVLDLLLVPFLTFTVMSILALFVIG
                     PIFHSVENYVLAGTKFVLNLPLGLSGLILGGVHQIIVVTGVHHIFNLLEAQLIAADGK
                     DPFNAIITAAMTAQAGATLAVGVKTKNKKLKALAFPAALSAGLGITEPAIFGVNLRFG
                     KPFIMGLIAGAAGGWLASILKLAGTGFGITIIPGTLLYLNGQIVKYLIMVIGTTALAF
                     VLTYMFGYEDKDEKVVAEVSPLVEETDDNPTITQTSQLRAETIVSPLDGQVIALDTVS
                     DPVFSSGIMGDGLAIKPSGNTIYSPVDGFVQIAFETGHAYGIKSDKGAEILIHIGIDT
                     VTMNGTGFTSKVKADQKVKKGDILGTFDSAKIAEAGLDNTAMIIVTNTADFADVIPLV
                     KEGVVTTGDMIIDVK"
     gene            22297..23736
                     /locus_tag="VBC52_001020"
     CDS             22297..23736
                     /locus_tag="VBC52_001020"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEO6882617.1"
                     /translation="MNLPTEIRYRPYDEWTEEDKENIVKNVSKSPWRATYHLEAKTGL
                     LNDPNGFSYFNGKFHLFYQNWPFGAAHGLKQWVHTESDDLVHFKETGIKLKPDHVNDS
                     HGAYSGSALAIDDKLFLFYTGNVRDMKWNRDPRQIGAWMTSDGKITKFDKVLISQPND
                     VTEHFRDPQIFNYDNQFYAVIGAQNSKKCGFIKLYKALNNDIHHWEFVGDLDFGGTGS
                     EYMIECPNIILVKGKPVLLYSPQGLDKNELDYQNIYPNTYKIGQYFDANSSKIVEPSP
                     IYNLDYGFEAYATQGFNTSDGRAFIVSWIGLPDIDYPSDQFDYQGAMSLVKELSINNG
                     NLYQYPVPAMKNLRQHQAEFKTQLQTNNTYELELLVPRNDLSSFVLFANPKGQGLSIT
                     IDTAKGKVIVDRSQAGQQYATEFGTSRQCDIPKDATSVNIFVDKSIFEIFINKGEKVF
                     TGRVFPDAEQSGIQLKEGHVHGKYFELKY"
     gene            23738..24700
                     /locus_tag="VBC52_001021"
     CDS             23738..24700
                     /locus_tag="VBC52_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6882618.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVTKVNEAMRTL
                     GYKPNNLARSLQGKSAKLIGLIFPNIRNIFYAELIEHLEIELFKHGYKTILCNSEKDP
                     IKEKEYLEMLGANQVDGIISSSHNLGIDDYEKVEAPIVAFDRNLAPHIPIVSSDNFFG
                     GKMAAQTLKKHGCQKMIMITGNDNSDSPTGLRRLGFSYESKESKVITVTNGLSNMRRE
                     MELKSIISTHKPDGIFTSDDLTALLVIKLISQLGLSIPEDIKVIGYDGTSFIQDYVPH
                     LTTIKQPIREIAQLMVEILLAKIEGQKTNKDYILPVSLIPGSSV"
BASE COUNT         7938 a         4934 c         3994 g         7902 t
ORIGIN      
        1 aaactttctt cgaaaacgtt ttcgaatata agtatcggag gttgtaatat gacaattaaa
       61 aatctcactg ttgcaggaag tggcgttcta ggtagtcaaa tcgcctttca agcagcttac
      121 aaaggaatgt ctgtcaccat atatgacatt aacgatgaag ctttaaataa gggaaaagaa
      181 cgcatcaaaa aattagctaa agtctatcaa tcagaaattg aaacagcaaa agaagcatat
      241 agtgataaag ctaaaagtat caagtataat aaaaacttat tgcctagctt agaccatatt
      301 tttctttcaa aagttgccga ttcattagac ttgattgctg atttaccaaa ccaaattact
      361 ttttcaaaaa acttagacca agctgtaagt gacgccgatt tagtaatcga agctgtccca
      421 gaaacagtta gtataaaaga agatttttat aaacaattag ctaaagtggc accttcaaag
      481 acaatctttg caacaaattc ttcaacactt gttccaagtc agtttgctga tataacagga
      541 cggcctgaca aatttttagc aatgcatttc gctaacaata tttggcaaaa taatattgtt
      601 gaaattatgg gacataaggg aactgacgac gaggttatca aagaagcttt ggcattctca
      661 aaagatattg gtatggtgcc tttacatatt cataaagaac aacctggcta tattcttaat
      721 tcaatactag ttccattctt agagagtgca ctagcattgt actatgataa ggtttctgat
      781 tctgaaacta ttgataaaac ctggaagtta ggaacaggag caccgatggg acctttagaa
      841 attttagata ttattggcat cgataccgct tataatatca tgaagaatta ttcagatacc
      901 aacagcgatc ctaatagtct tcatgctcac cttgcaaaaa tgcttaaaga agaattcatt
      961 gataaaggac gtaccggtaa agctgctgga cacggctttt atgattacga ctaaactata
     1021 aaagaggtta gataaaaatc taacctcttt tataattcaa ctaaagaata gttgcaccaa
     1081 gaacagtttt aaaatgattt aaagcccatt gatgattact atcatttaag ctagctacag
     1141 ctgctgctgg gacctcaatt ttataaccta agttataagc atcaattgct gtgtgtaata
     1201 cacaaatatc tgtcaatact cctgtcaata tcaaagtatc tactcgtctt tcacgtaggc
     1261 gaatatctaa atcagtgcca gaaaaggcag aataatgacg cttatctatc caaaaaacac
     1321 gactatcttc cttaatagtt tcatatagag ttcccaaagg accatatagg tttctaccag
     1381 aagtcccttt aatattatgc tcaggaaaaa gattgctctc tggatgaaaa acatctccaa
     1441 tatcatgatt atcaatcgca aaaaaaatat aatcccctga ccggtatgct ttctctgtaa
     1501 cgtcagctat agctgaagcg atagattgcg ctggtttacc tgccgttaat ttcccatcat
     1561 ctgccacaaa atcatatgtg tagtctattg agatgagtgc ttttgtcatt tttcttctta
     1621 tctcctcttg atttcaaaaa aggaactgaa agctactccc agtcccatgc tatgattaat
     1681 tatattcttt taatttgtca aaaatacctt cgttaataac tttgaggtat gttcccttca
     1741 taccgagtga gcggctctca atgattcccg cagactccaa tttacgaaga gcatttacaa
     1801 taacggaacg tgtaattcca atacggtctg caataacaga tgccgttagg cgtccttcaa
     1861 gtccatctaa ttctcctaag atagcagcta cagctttcat ttctgaataa gatagagtgt
     1921 tgatagccat tgtcaccgct gtttgtttac gaatattttc ttctagattt tctgtttgaa
     1981 ggttaagtaa ttgaatacca acaacggtgc ttgcaatttc aactaaaatc aaatcatcgt
     2041 cgctaaattc tttatcatta cgccagataa taaatgttcc caagcgcatt ccaccaccat
     2101 aaattggagc gattgttgta ataccatctt ggaagttttc ctttgtctca actgggaaaa
     2161 ttgacaaatc attatctact gataagttag cttctgtatc ataaacacga ctagcagatt
     2221 taacatagta atctgggaat tgttttgttt caaaaaattc ttccacacgg tcagtgtttg
     2281 ttttatattt catggcatag ccaagtaagt taccaccacc attaataata catgcgttac
     2341 agtcaataat atcagctaat tgagccgcca ttgtattata tggtagttcg gcatctaagc
     2401 tatctacgga acgctgtaag atggatgtaa tttttctagt tttttctaat aaattcggca
     2461 tttccttcat cctttgtatg ttttgcgtta caattatatc aaagcatttt agcttttgca
     2521 aatcgatatc agtacatttt aagaacttct attgttaatt taatgtttat ctaataaaaa
     2581 ttcattctag cgcttgtatc ggcttaaaaa gcgagccatt ttttcttgta gttcagtcaa
     2641 ttcatcaaca cgtggtaagt aaacaattct aaaatggtca gcggtattca tattaaatcc
     2701 tctaccgtgt gttagtaata ctttttcttg ttttaagaaa ttaagcacaa actcttcatc
     2761 attatcaata cggtacatat ctgtatcaat tttagggaaa agatacagtc cagcatttgg
     2821 ttttacagca gataaaccag gtatttcatt aattgcttta tggatataat tacgttgctc
     2881 gtagatacga cctcctggca acaacatact atcaatagat tgctgtccgc ctaacgatgt
     2941 ttgaataacc tgttgagcaa gaacatttga acacagcctc atgttagcta acatattcaa
     3001 accttcaata taacctttaa catgttgtcg cggaccagac aataccatcc aaccaacgcg
     3061 gaaaccacag atgcgatgtg atttagataa gccagataaa gtaactgtaa aaatatcctc
     3121 tgcaatagaa gcaattggga tatgctccat tccatccata actaaacggt cataaacctc
     3181 gtctgaaaaa ataatcaagt cattttgtct agcgatatcc acaatctctt gcagaatttc
     3241 acgcggatag actgctccag ttggattatt tggattaatg aggacaattg cttttgtttt
     3301 ggatgtgatt tttgatttga tatcatcaat atcaggatac caattagctt cctcatcaca
     3361 aatataatgc acagcatttc caccagcaag gctaacacaa gctgtccata acgggtaatc
     3421 tggcattgga actagaacct cgtcatcatt atcaagcaaa gcttgcattg acatagagat
     3481 tccctcacta acaccattaa caatgtatat atcatccatg tcaacatgga tgttttgtaa
     3541 ctggtaatac tgcataacag ccttacgagc tgaaaaaata cctttcgaat cagagtaacc
     3601 ttctgattca cgcgcattcg taattaaatc acgaatgact tcatctggcg cttcgaagcc
     3661 aaaggcagct ggatttccag tatttaaccg caatatcttt tcaccatttg ccctcatgcg
     3721 gtccgcctct tctaaaacag ggcctctaat atcataagcc acatgttcca gcttcattga
     3781 tttatcaaat atcttcataa aaatacctcg tatcttccat tataaaacaa atattctcaa
     3841 aattgaaact tttactgcgg tttatcaagc gattaagtta atattaagct atttcagaaa
     3901 actaacataa aacgctttca tttttctaga aaaaacgtta taatatagct ataaaatgaa
     3961 taaccaacat atctaactta ctcatcactc actccttctg ataagatggc tagttgagtg
     4021 caagaggtta tctcttttgg ttatatttag aggaggacag gcttatgtca caaaaatatg
     4081 aacgcatcct tgtcgctatt gatggatcaa cagaatctga acttgctttt gaaaaggcag
     4141 ttaacgttgc tcttcgcaat gattctgaat taatcttgac acatgttata gatacacgtg
     4201 ctttacaaag ctttgcgaca tttgatactt atatctatga aaaattagaa aaagaagcaa
     4261 aagatgtcct tgaggaatat gaaaaacaag ctcgtgaaaa aggagcagat aaagttagac
     4321 aagtcattga atttggtaat cccaaaacat tacttgcaca tgatattcca gaaaaagaaa
     4381 aagtagactt gattatggtt ggtgcaacag gtcttaatac ctttgaacgt ttccttatcg
     4441 gatcttcctc agaatacatc cttcgtcacg ctaaagtaga tttactcatt gtacgtgacc
     4501 ctaataaaac aatgtaaatt taaatacgct cactttctaa aatatcctga gttatattaa
     4561 ctcaggatat ttttattttc gatttaagga tttctgaatt tggctatcta attcacgtcg
     4621 aattgatggc tcaggagcga aatgttcttc ccagtcgtct ggttttaaaa ttttatgagt
     4681 aacaggatca aaatgagctt tgccatctgg aaagattttc cccatattgg cctcatgtac
     4741 tgtatcaaaa agaggttttg ggtcgactcc cataagaaca aaggagccat aagtcagata
     4801 aagtaaatct gttaaggcat ccacttctcc cactaatggt gtttctggat ggtctttaga
     4861 aataacctta tctcttgctt tatcaatagc taagtgtaaa ttacggatag attggtcaaa
     4921 tactttttgg ttaccttgac tagcagcata caagaactca acgatttctt ctactttaaa
     4981 gtctgacctg aaaccagctt ccttactgtc atataagcgt ggcgtttcaa tagtttcacc
     5041 atccattaat agatggaagg attttacttt attgaaattc tcgtcacgac tactgaatgt
     5101 tttttcaatc tcaaattgga taagtccata atgggctaat gccttagaga taccatcatc
     5161 attattgtca gcagttgtat aacgtgcatt ttctctcact tgctgactag cgttccccat
     5221 ggcaacacca atgccaactt gagataacat ctcaatatca ttatcagaat ctccaaatgc
     5281 tatcacttcg gatagatcaa aaccatagcg ttgaccaaca cgttcaattc ccttaacttt
     5341 tgactgccct tcagaaatga catccattga gtaaggactg cttcttgtta atttaacacg
     5401 aggaaattgt ttttgaattc gctctgattc gccctctgtt gcaactaaaa cgacctgata
     5461 aataggttct cgtaagataa ccatgaggct atcaatattt tgaggtttaa tccgacgaat
     5521 aaaatgctta aagcttcgtt caatcgcttt tgcccatttt ctcggaacaa ggctagatac
     5581 aatctgcccc aaacggctag ttcctagacc aataatacct gaaccgagta atcctgaggc
     5641 tgttcctaat gaaatttctc ttcttcttgc tccagcataa cgaatcagac gatagacagt
     5701 tgtcttagat aattgattgg tataaataat ttcacttcta ctatagatat attgaccatt
     5761 ataggttaca gcaaaatcta aaccaaggtt ttctaaaaaa ggttgtacaa aactaggacc
     5821 tcgccccgtt gctaaaccga ctagtattcc ttggtctttc aaatttcgaa ttgctttaat
     5881 tgttgacttt tgtacattct tacgatcatt aagaagtgta ccatcaatat caaaaaatac
     5941 tgccttaatt gccactgggt caacatcccc tcttttttat gataaaataa attatatcac
     6001 aacttgaaag aagtataaaa tcaatgaaaa aaatccttgt tctacacaca ggtggaacga
     6061 tttccatgaa tgctaacgag aagggacagg tcatgtccag cgccgataac ccaatgaaat
     6121 acgttgattt atcattagat gaccttgatt tgactgttgt agattttcta aatcttccca
     6181 gtcctcaaat cacccctcat catatgcttg atatctatca ctatctgaag caacatgcca
     6241 gtaattttga tggtgttgtc attacgcatg ggactgacac tttagaagaa acagcatatt
     6301 ttttagacac aatgatactc cctaaaatcc caatcatcat taccggtgct atgagaagca
     6361 caaatgaact tggtagcgat ggcgtttata attacctatc agccctccgt gtcgctaata
     6421 gtactaaagc tgccgacaag ggggtgctag ttgtcatgaa tgatgagata catgctgcta
     6481 aatatgtcac aaaaacacat acaacaaacg tttcgacatt ccaaacccct acacatggcc
     6541 cccttgggat tattatgaaa caagacctgc ttttctttaa agcaactaaa gagagagttc
     6601 gttttgattt agataaaatt acaggaactg ttcctatcgt gaaagcatat gctggcatgg
     6661 gtgatagtgg tatcattagc tttttaaatt ctcaaaatat tagcggatta gttattgaag
     6721 cactcggtgc agggaatatg cccccaaaag ctgcacaaga aatcgaagaa ttaattgagc
     6781 aaggtgtccc tgttgttcta gtgtctcgct gttttaatgg tattgctgag ccagtttacg
     6841 gatatgaagg tggcggagca aaattacaag aatctggtgt catgtttgtt aaagaactaa
     6901 acgctcccaa agccagactc aaattactca ttgcactcaa tgcaggtctc acagggcaaa
     6961 atctaaaaga ctatattgaa ggttgataaa aaactctaga aatatttcta gagtttttta
     7021 ttaattttca gtttgtaata ctctctcaac actgctaata ggcatttctt gattcgttat
     7081 taattgaaat gcttctgctc cttgataaat cagcatccct agaccattat aagcatgttt
     7141 cactccattt tgtctaacaa atcgtaacaa tgctgtttct ttaggcttgt aaaccaagtc
     7201 gacaactact aaattcggtg ttaaaatttc tggatcgtta attaaactat aattatctaa
     7261 tggcctcatt cctaaactag tagcatcaat ataaaaatga ctagttctaa tagcgtcttt
     7321 aaatgctgtc ttattttcta gataatcaac gactaccttt atttgaaact gttttttaat
     7381 tttatctgat aagtcaatga ccttatcgta atttgagctg ttacgattaa ataatctaat
     7441 ttccgcaact ccctccatag ctgcttgaac tgcaactgct ttacctgaac caccaatacc
     7501 agctattgta attattttat ttttagcact gaaaccttga gctgctaaag ctttaaaaca
     7561 accaatgcca tctgtcatat ggcctactaa acgtccggtt ccaccttgat taacgatagt
     7621 atttacagca cccactaatt tagcttgagg agataaatca tctagcaaag ggataacact
     7681 ctgtttaaat ggcattgaaa cattaacacc acgaataccc aatgccctga cacctcgaac
     7741 agcttctgtt aatttaccct cttctacttc aaatgtcaga taggcataat tcatgttttt
     7801 ttcttgaaaa gaggtattcc acattaacgg ggatagagag tggcgtgcag gattggcaat
     7861 tagaccagtt agtaaagttt ccccatttaa tgaattcatt gtataacctt ctttcttaac
     7921 aatttatttc agttcatttt accaaaaact gactcaatta aaaagagagt atgtaattta
     7981 tactctctcc cttagtttaa ggcaatatat tacctgcact acggtaaata tcataccatt
     8041 ctttacgggt taaagcgata tcattagcct tatcaatcgc ctttaaacga gatggattca
     8101 ttgaaccaac aatcgcctgc attttggcag gatgacgtaa aatccatgca atcacaatag
     8161 cttcgctgct aacattgtag ttttttgcca gtttagctat ggtttcattt aattccttat
     8221 aatccggatg attaacaaat aaaccacgag ataaatcaat ttgaaatgga gaccaagctt
     8281 gaatggtaac tcttttaaga cgacaataat caacaatgcc atcatcatgt tcaattgaag
     8341 ctttatttaa catgttaaca tttaaccctg catcaaacat tggtgtatgt gctggcgata
     8401 actgaagttg attcactgct aagggctcgt caaggtagga ttgtaacaac tccatttgaa
     8461 accggttttg attgctaaca ccaaagtgtt ttactttacc ttctgccctc agtttggtaa
     8521 atgcttctgc tacttcttca ggctcaacta atacatctgg acgatgaaga atgagaaaat
     8581 ctaaatattc tgtccctaat ctttctaaaa taccatctac tgatgataaa atatactctt
     8641 tagaaaaatc aaaataacct tctcgtatcc cacatttaga ttgaataatc attttgtcac
     8701 gattgacatt taaatgtttg attgcatctc gaaagcgtaa ttcagatagc cctcccccat
     8761 aaatatcagc atgatcaaaa aaattaatac ccaaatctaa tgctgttcca actacttctt
     8821 cagcttgttt tcctttcaag tcactcattc tcatacaacc taaagcaatt cgagttgctt
     8881 gaatgcctgt ttgtccaatg ttttgtaaca tatgcacccc attctaatct gtttgtatta
     8941 acagaaagat taatcaattc attataacaa aatttctatg aaaaggctta ctattacaaa
     9001 tgcaaaaagc tgagtaaatt taactcaact ttttttctga tactcattat tgactaaata
     9061 agtcggtaag aaaaatctat tctgaaaagt actattataa attacagtaa tcattattgt
     9121 cataacatac ctccacatct gtgataggaa tatcatatga cagcaagctt aaactgagct
     9181 tagcacagcc ttaatagaaa cttaatcaaa atctgaatgt tggcgtaaat tatctagaat
     9241 aagtgcccat tctgtatttt ctttccaatt attatctttg acaatatcac tagcaacacg
     9301 tctggcttct tctaaaatag gataatcctc aacgatatca gctacttgaa attctggaat
     9361 tccagactga cgagtaccaa agatttcacc agaaccacgc atttttaaat ccgactcagc
     9421 taaaacgaaa ccatctgtcg tttctgtcat gattgtcatt cgttttttcc cagaatcagt
     9481 tttgggatta gccactaaaa cagcgtatga ttgtttatat ccacgaccaa cacgcccacg
     9541 aagttgatgt agctggctta atccaaaacg atcggcatcc ataataatca tgatcgttgc
     9601 atttgggaca tttaccccta cttcaataac tgttgtagat actaaaatat gacttttttt
     9661 atccttgaaa tcttgcatta tagcatcttt ttcatcattt ttcatacgtc catgtacaag
     9721 agcaacctta gcaattcctt caaaataagt agataattca gcatgcaatg ctactgcatt
     9781 ctttaaatct aaagcttctg actcttcaat caacggtgaa atgacataca cttgagcatc
     9841 tttttgcaat tcacctttta cccattccaa cacagtacct agctgctcgt gtttcaccca
     9901 gcgtgtaata ataggtttac gaccggcagg taattcatca ataatagaaa catccatttc
     9961 tccaaaagct gtgattgcta gagttcgggg aattggggtg gctgtcatca ttaaaacatc
    10021 aggattttct cccttttcac ggaaaattct acgctgttta acaccaaaac gatgttgctc
    10081 gtctgtaatt acaagcccca gtttatggta ctgtaccgag tcctggataa gagcatgagt
    10141 tcctacaatc atatcaaccg agccatttgc aatagctgct aaaaccgtac gcttaacagc
    10201 tgctttcata cccgaagtta atatagcgat tgataaatct ggaaataact cttgcagact
    10261 aatgtagtgt tgttcagcta ggatttccgt tggaaccatc aaagccgatt gaaaacctgc
    10321 tgtataagct gcatacattg atagaccagc aatgactgtc tttcctgatc ctacatctcc
    10381 ttgcaataaa cgattcatat gagctcccga tgacatatca gataatattt catctaaaga
    10441 gcgcttttga gcattcgtta gaataaaagg taaagaggaa atttttgtct ccatagcatg
    10501 tttactataa agaataggca aaccatttgt ttcagattta ttttcggatt tcaaaacttg
    10561 aaggttcatt tgaaagtaaa ataattcttc aaatttaatc cgacggagcg cttgcttata
    10621 ctctgtgata tcttttggga aatgcatagc taaacaagcc tgactacgac ccatcaatcg
    10681 gtatttttcc aataaagtag ctggtaaatt ttccttcaat tccaaatgcg cactgatttc
    10741 aaaagctgac ttaatagctt taatcaaagt agactgtgaa attccctgag ctacgcgata
    10801 aacaggttgc atgtcatctt caacttgagc taaaaccttc atcccagtaa tagctgattt
    10861 agttgcatcc catttaccaa agacagctac ctcttgacca agttctattt tatcagctaa
    10921 atagggttga ttaaaaaaac taacatttaa gacagcttcc ccttgacgca acttaaaact
    10981 taaacggtta cgtttaaaac cataatattg tacattagct ggagtaacga ctaggcctgt
    11041 aatgactgct ttttcaccat ccactaaatc aaaaacagat tttgatttaa aatcttcata
    11101 gcgaaacgga taataaagaa gtaaatcttc tactgtataa atatctaatt tctgaaattt
    11161 ttctgctgat tttggtccaa atcccttaag attagaaatt ggtgattgta atagcataag
    11221 attctccttc taatatcttt tattatacta aaagatcacc aaaaaggcgc ctcaagagcg
    11281 cctttattat gtctaattat ttatggccaa taacataagt taattgtgca gcttgtgctg
    11341 aactaaaagt acggtaagag acaataccac gtcccattgc attagattct gaaataagaa
    11401 ctgaaccatc ttttttaact tcttcaacaa tagctacgtg accgtaagtg ccatcagcac
    11461 cagcttgtcc tggtgaaaat gatacagcat agcctacttt tggtgaatgt gttgtttcaa
    11521 aaccagcctt atgttgccaa tctccaccat tacccataaa tggatcaaat gtataaccta
    11581 actctttagc gcggttaaag acataccatg tacattcacc ccatggataa gttgatgcaa
    11641 tatagctagc tgtgttaact gctgttgata agctaaaacc acttggtaaa tctgctaaac
    11701 ttgctgtcga taaagctttc aaggcagcag gaatttctgg tgtccctcca tgttgatcaa
    11761 tccagcgatc taaatcttcc ataatctggg cacgacgttt accagttcca gagttatctg
    11821 tataataaac accaccctca gtaactgtat taagttgtcc agaagataat ttctgtgctt
    11881 tcaaatcttg gatttcaaag ctagagttgt tgtttttaat aattgtattt tttgtcaaca
    11941 acataattgc ttcttcatca ttataagcac tagcatggct agagtcatga tcaaaggcac
    12001 cataaccaaa catattagca cctggcatct tagccactcc ttgagttccc aatgaacttt
    12061 caaggacagc aatagctaca atagcacgga catctaaacc actatctttt tcccattgta
    12121 ataacttagc accattgata cgattagaat catattgaat ccctaaagat tttaagtacc
    12181 catctaattg ttcagctttg ataccgtaac gatgtgacaa taaatagtga gagtaggcat
    12241 cctgaccaga ccagtgttct acgtagaatg gcgttgctgt cactggtgaa atcggtgttg
    12301 ataataagtt atcatcaaag gataaagcag gaacagaagc attctgattt gtctgaacac
    12361 tttctatctg agacggagcg ggagttattt taggctctac taatggtaag gtaggctcag
    12421 gttttaattc agtctttgtt gattgacctg gcttactgtc tgcttgacta ctgtttgtag
    12481 tattagtgct aggagtagat gctgttgaag cagaatcaga tgttttagca tcttcgttgg
    12541 aagaagattg atgtgatgaa cttgatgatg attcattgct tgatgatgtc tctgtcgtac
    12601 caatctctgt tttttctgtt gagatacttc cttcggctga aggtgaagca gtttctgaat
    12661 ctgtaggttg tgttttttcc tgtttctctg cttcagaaga actttctgtt gatgctgttg
    12721 ccattgtcag gcttgtttct ggattaatag tttctgctaa ggtcactaaa ggtgatgata
    12781 gcgataagac taaagtggat aacaacaaag ttgccattac tttttgtttt gacatatact
    12841 aatgatttcc ttctataaaa attgtcactg attagtttaa catattttta aattttttaa
    12901 aacagttaac tactgtgtca tttgttttta ataatatctc ggaatacgat cgcttaaaag
    12961 acaaagtact tcataattaa tcgtctgtct tttttgagcg acgtccgtag ttgtaatgtt
    13021 acaactacct tgttgaccaa tcaaggttac tttagttccg atagtatatt tttgaggtaa
    13081 acggatagtc atttgatcca tcgagacacg cccaataatt tcacaaagtt ctccatttac
    13141 tataactgaa aatccttgca tatctcgtgt ccatccatca gcgtagccaa tcggtactgt
    13201 tccaacaaat tcgtctccag taacttgata ggttgcgcca tagccaactt gagaaccatc
    13261 gtgtagttgt ttaacatgta ctagctcaga ctctaaactt aaagcagggt ttatagggta
    13321 aggtaagtct aaatcagttc cacttggatt taagccgtac ataacaacac ccaatctaac
    13381 ggcattaaaa attgtctcac tatgccaaag gcttgtagca gaattactag catgaacaag
    13441 gtccggacaa ttatctaatc ctgaaataaa gtctttaaaa aaagtaagtt gttgattgaa
    13501 tttacagtta tcggcttcat cagctgttgc aaaatgggtg aaaatcccct tgacagaggc
    13561 accactttcc cccaattcag aaattagctt atttccttca tctaactggc gaacacctat
    13621 acgtcccatt ccactatcaa ctttgatatg tacttctaga ccagataaat ctactgctga
    13681 tgtctgacat aaacgtaacc actccaagct agcaactgtt agagtaatat tttcattttt
    13741 ggctagtata acctgctcag gcataacaac acctaatacc aatatcattt ttacaattcc
    13801 tgcttgacgt agctcaatag cctcatctaa attggatacg caaaaaccat ctacaataga
    13861 ctcaatgtat tttgaaactt caacagcacc atgcccataa gcattagcct taactacagc
    13921 aaatgttttt ttatcacttg gaatgtgctc ttgtacagat ttaacgttat ttgcaatcgc
    13981 ctctaaatct atcaaggctc tggttggtct atgataactt gaaatcattt ttcttcctcc
    14041 aaaataacac tagcttgtgc ataattacca ctatgagata ttgaaacaaa agattttcca
    14101 ttaaacggtt cttttgtaat caaaggcgct cctttatcat ccgataaaat ttcgatatca
    14161 tgaaaattaa ctttcccaat tcctgttcca agtgctttgc tataagcctc ttttgctgcc
    14221 catcgccctg ttaaaaaaga catctgacgc ttgggattgg aaattccttt aaaaagaagc
    14281 aattcttgtt cggtcaaaac gcgttctgca aaacgttgat tacgctcata tgctttagta
    14341 atcgcctcta tctcttgtaa atcaattccg tgtccaacaa tcatgtgtaa ctcctataag
    14401 ttcgatactt acaaactatt gtaaatatcc ctaattagca tttctgtttt atcccaacct
    14461 agacatggat ctgtgataga cttaccaaaa acatctggtt tatcttgtct tccatcttct
    14521 aaataagatt ctatcaaaaa accacgaaca tagtttctaa ttttagtatg ccactgtcgg
    14581 tctgcaagta ctgatttcac aatgcgaatt tgttctaaaa attgcttacc tgaattgtca
    14641 tgatttgtat caacaacaat aaagggattt tgcaaatcag tctcttcata atgagtaata
    14701 gtttctaaaa ggttttcata atgataatta gggatacttc gatagtcggc attatgatat
    14761 cctctcaaaa taacatgtga cagcaaatta ccatctgttc taacttgctt attttgatag
    14821 aataattcct gttgattttg tgctgcatag accgcattaa acatgactct taaattacca
    14881 gacgttggat ttttcatacc tactggtgca tctatacctg aagatataaa acggtgtccc
    14941 tgatcttcca ctgaacgcgc accaatagca tagtaagaaa ctaaatcatc catcactgat
    15001 aacatctctg gataaagtaa ttcatcagct gtcaggagtc cagtctctcg aatgatgtca
    15061 tagtgcatct tacgtgcttg aaaaaaaact cctagtttgt cagaatggtg aactaagcct
    15121 ttataaccat caccattcgt tcttggctta gcggtataaa ctctcataac caaaaacatt
    15181 ttatctttaa aggcagcttc caatttagct aaacgctttg cataagttag caccgccttt
    15241 ggattatccg cagaacaagg acctactatt actagaaacc gctgatcctc accttttatg
    15301 atatttttta actcttcttc tttggctaat tttttttggc taacttctag atctaagcta
    15361 gttttctgct tcaatatttc aatattaatt ttatcactta actgatgaaa tcccatgttt
    15421 tttcctaata caacctaagt ctattaataa gctctgctat acaatatagt cgtcatacta
    15481 ttagacaagt aatgataaaa cagtcttctc agactgtttt atttttattt taaaatcatt
    15541 gttattgtaa acatctcaga ccatgacaat ttttaaactt catgccagag ccacaaggac
    15601 atttatcatt acgtttaatc ccttggtatt ctggtgattc attattcata ggtacatgtt
    15661 gtgccgagat attttgctct gctgttgtcg tagcatgttg agaagcacgt tcacgctctt
    15721 gttgatgaat ttgagctttc atcaatgtac gtgtcacatc aaactcaatc gaaccaatca
    15781 tatcttggaa catacggaaa ccttctgatt gatactcaac gattgggtta ttttgagcat
    15841 aaccacgaag tccaacggag ttacgtaatt gatctaaggc atcaatatgc tctgtccatt
    15901 gattatccac taccatcaag attaagactt tttggaattc gataatagct tctggatctt
    15961 ttaatttagc aatttgttgc tcataaacag ctaaggcacg ttcataaaga agttctttaa
    16021 tttcagcctc ttttagacca cgaagttctg aaactgtgat agcttcttca tctaataacg
    16081 ccgatcttgc aaaattaaca atagcttctg ctgcagtatt tttatcgctt ctgctatgtg
    16141 catcaactgc tcttttaatc gtacgtttaa tcatcccttt gagctctggt cctaaatcac
    16201 gctcagcagt aatcacttct ctacggttag cataaataat ctcacgttgt tcacgcataa
    16261 catcatcgta ttgcaagact tgtttacgtg tgtcatagtt attaccttca acgcggcgtt
    16321 gagctgattc aacttgtcgt gtcaacattt ttgatttaat aactgtatca tcttcagcta
    16381 aattcattcg ctcaagaacc actttaatac gatctgtacc aaagcgacgc atcaaatcat
    16441 cttctaacga aagatagaat tgtgattcac ctggatctcc ttgacgaccc gaacgaccac
    16501 gaagctggtt atcaatacga cggctttcat gacgctctgt accaataaca cataaaccac
    16561 caagttcacg aaccccttca ccaagcttga tatcagtacc acgaccagcc atgttggttg
    16621 cgattgtaac agcaccacgt tgcccagcat tcataatgat ttgagcttct ttaaagtgat
    16681 ttttagcatt caaaacttca tgtggtactc cagcagcaac taattttcta gaaattaagt
    16741 ctgacgtttc aacagctact gtaccaacca gtacaggttg tccttgttca taacgttctt
    16801 tgacatctgc taccaccgct ctaaatttag agtcaagagt tggataaagt agatctgaat
    16861 gatctatacg ttgaacagga cgatttgttg ggataggaat aacgcgcata ttataaattt
    16921 cacgaaactc ttcttcctca gtcttaccag taccagtcat tcctgctaat ttatggtaca
    16981 tacggaacat attttggtaa gtaattgaag cgctcgtttt tgattcttct tggattggca
    17041 ctgactcttt tgcttcaata gcttggtgta agccatctga gaaacgacgt ccttccatcg
    17101 tacgaccggt aaattggtcc acaattaaaa tctcttgttc ttcactaacc acataatcaa
    17161 tatttaacaa cataatatag ttagctcgaa gagcgttatc aatgtagtga gttaaggcaa
    17221 cattttcgag atcatacaag ttatttagat ggaaatagtt ttcagccttg tcaatacctg
    17281 tatctgataa accaattgtt tttgttggaa catcaatgat ataatcatcg ctattaagcg
    17341 tttttacaaa catgtccgca cgtgtataca gttggttcat ctcagagctt acaggacctg
    17401 aaacaatcaa tggcgtccta gcttcatcga ttaatacaga gtcaacctca tcaaccaaag
    17461 catagttgag tgggcgttgc accatatctt cttggcgaac aaccatattg tctcgaaggt
    17521 agtcaaatcc gacttctgcg ttagttgagt atgtgatatc acaattataa gcttcgcgtt
    17581 tttcaaatgg tgacttagct gctaagttaa tccctacaga taatccaagc caactataaa
    17641 cctcacccat ttcggtcgca tcacgtgttg ataagtactc attaacagta ataacatgaa
    17701 cacctaatcc tgagatagca tttagataaa caggcattgt cgctgttaaa gtttttccct
    17761 caccagtacg catttctgga atatcaccat ggtgaagaac gataccaccc ataatttgga
    17821 cgtggtatgg gtataaacct aatacacgtt tagaagcttc acgaactacc gcaaacgctt
    17881 ccggtaacaa ttgatctaaa gtttcaccat tttgataacg ttctttaaat tctggagttt
    17941 ttgcttgtaa agcttcatct gataacgcag ccatatgatc tgcgtaagag tcaactttct
    18001 tagctatttt atctaatttt tttaattcac ccttgtcatt ttcaataaca gtgcgtagaa
    18061 tatttgccat tctaattcct ttcgatactc ttatcaccac attttaccat aaataatgtt
    18121 gtctttcaat aaatggtcgt tttaatcgag attaaaaaga gtggtatccc cactctcatt
    18181 attttttaac atgacttgct ataatttcta actgtccatc aatatcccat ttgacaacat
    18241 catttggcaa gataaagtgg tcaccttttt taagtgtata aactttgtta tctactgtaa
    18301 tgtgacccgc tccgtctaag acactaacca aaaggtaggg tgcaaattgt ttaaagttgg
    18361 taacaccaga aatttcccat ttataaactg taaagaaatc atttgaaact aataaagttg
    18421 aagatagatg ttttaacttc atattggcag gaacagtatt ggcaggttta ccaatcgtga
    18481 gaacatctat tgattgttca atgtgtaaat cacgtaattt tcctgaagca tctggacgat
    18541 caaaatcata aacacgataa gtagtatcac tagattgctg tgtttctaaa attaatatcc
    18601 ctttacctat agcatgcatt gtcccacttg gaacgtagta aaaatcccct gatttaacag
    18661 gaattcgggt taaaagatgt tcccaatctc cagattcaat catttggcga agttcttctt
    18721 tagttttagc attatgacca tagataattt cagatccttc atcagcagaa ataatatacc
    18781 aacattctgt tttaccaagt tcaccttcat gtttaagagc ataagcatca tccggatgaa
    18841 cctgtacact aagccagtca ttagcatcta agattttagt tagtaaagga aagacagtat
    18901 catcagggtt gccaaataag cttttttcac cttgatacag cttatctaaa aaacaacctt
    18961 taaagcgacc attcttaact cttgagacac cgttaggatg agctgaaata gcccaatatt
    19021 ctcctgttgt ttcacttgga atatcatagc caaattcatc ccttaacttt gttccacccc
    19081 agattttatc gtgcatgctt gcttctaaaa ataatggttc cgacataaga atcccctatc
    19141 ttttgtaaaa ttcaagccta ttatagcatt ttatccatca tatcgcttac tttttttaat
    19201 cttttatttg ctaactaaac taagtagtgg cttaagggtt ttattaacct tttgatattt
    19261 tctttgctaa agcaaaattt cctaacgttg cagaaccatt ttcttctata gctggtgtca
    19321 caatataatc agacaaatcg ggaactggta aatacccatt taataaagtt gcaaatgtct
    19381 ggcgaacacg tcttaacatg tgttcttgtg ccatgacgcc tccaccaaat acaatgactt
    19441 gtggacgata taatacagtt gcttgcatcg cagcctgagc aatataatat gcttgaacat
    19501 cccaaaccat tgaattttct tcaatcaatt caccacgaat accagtccta gcttctagag
    19561 ttggaccaga tgccaaacct tctaaacagc tattatgaaa cggacagata cctttaaaat
    19621 catttgcttg gtcctgcgga tgcatcgcca tataagtatg acctgcttca gtatgaccag
    19681 ttcctcctat aaattcaccc ttttgtatag ctcctgcacc aattcctgta ccaatggtgt
    19741 aataaacaag tgaatcaata ttgttacgag ctataacttc accataggca gagctattaa
    19801 catctgttgt gaaataaaat ggaacattga aatctttaga aattaagccg agtaaatcaa
    19861 cattagccca atgtaacttt ggggtcgttg tgatataacc gtatgtcttt gattttttat
    19921 caatatcaat ggggccaaaa gaaccaatag caactgcctc taacttcttt tcaaatcttt
    19981 taaagaaatc aacagtcttt ttaatcgttt cttgaggcgt cgttgttgga aattgcattt
    20041 tttctacaac ttttaattcc tcatcaccaa ctgcacaaac aaactttgtc ccacctgctt
    20101 caatactacc atataacttt gtcatctttt ttcctatcta taatctctat ctcaaagaca
    20161 aaggttggga taattatccc aacctttaat cctcacttaa catcaataat catgtcacct
    20221 gtagtaacaa caccctcttt taccaatggt ataacatcag caaaatcagc tgtattagtc
    20281 acaataatca tagctgtatt atccagacct gcttctgcaa tcttggctga atcaaaggtg
    20341 ccaagaatat cacctttttt cactttttgg tctgccttta ctttagaagt aaaccctgtg
    20401 ccattcatgg tgacagtatc aatacctata tgaatcaaaa tttctgcacc tttatctgat
    20461 ttgataccgt aagcatgacc tgtttcaaaa gcaatttgga caaaaccatc aacaggtgag
    20521 taaattgtat taccgcttgg tttaatagca aggccatctc ccataatacc tgatgaaaag
    20581 actgggtcac ttactgtatc aagagcaata acctgtccat caagagggct aacaatcgtt
    20641 tctgccctta attggctagt ttgagtaatt gttggattgt catctgtttc ttcaaccaaa
    20701 ggactaactt cagctactac tttttcatct ttatcttcat atccaaacat ataagttaat
    20761 acaaaagcca aagccgtagt tccaatcacc ataataaggt acttaacaat ttgaccattt
    20821 aaataaagta atgttcctgg gataatggta ataccaaaac cagttcctgc taatttcaat
    20881 attgaagcta accagccacc tgctgctcct gcaattaatc ccataataaa tggcttacca
    20941 aaacgtaagt ttactccaaa tatagccggt tctgtaatac caagtccagc tgaaagcgct
    21001 gccggaaaag caagggcttt taatttttta ttttttgttt taacaccaac tgccaaagta
    21061 gcacctgctt gtgctgtcat agcagctgta ataatagcat taaatggatc tttcccatca
    21121 gcagcaatta actgagcttc aagtaaatta aagatatgat ggacacctgt aacaacaatt
    21181 atctgatgaa caccacctaa aataagacct gacaggccaa gaggtaaatt aaggacaaat
    21241 tttgtccctg ctaaaacata gttttcaact gaatggaaaa taggaccaat gacaaaaagt
    21301 gctaaaattg acataactgt aaaagttagg aacggcacta atagtaaatc aagaacatct
    21361 gggattttct tatgcaacca tttttcaagt ttagcaccaa gtaaacctac aaagaatgcc
    21421 ggcaatactg aattttgata accaactact ggaataaagc cgaaaaattt tatgggatgg
    21481 gcatcacctg aggccactgc ccaagcgttt ggaagggcag agttgaccat catcaatcct
    21541 aatacgagac cgataacagg atttccacca aaaactctaa aagctgacca agaaatcaag
    21601 gcaggaaaaa aagcaaaggc cgtgtcagtc aaaacgacag tataggtata aaaattagaa
    21661 cttgaaaatg cttttgaagt cgtcccaaat aaagctaaaa ctgtatcatt attaatcgca
    21721 ccgcgaattc ccataaataa accagtagca acaatggctg gcaacaatgg aacaaaaaca
    21781 tcaccgaaag tacgaatagc tcgttggaaa gcattccctt gtttagccgc ttctgccttt
    21841 tgatcagagg ttgacgaagt tggcaaacct tgagctacta cttcatcata aatcttatta
    21901 acagtaccag ttccaaaaat aatttgatat tgacctgaat tgaaaaatgc tccctgaact
    21961 ttttcgatat tttcaacagt atttttatca atgactgtct catcttttac cataacgcga
    22021 agacgagtgg cacagtgtgc tacgctacgc acattactag caccaccaat cgcattaatg
    22081 acttgtttag caatttctgt atttgaagcc atctataaat ctcctttgat gttttgaaag
    22141 cgtttcattt tatttactct aatattctag cacgtctcat aaatatatgt caagcgtttg
    22201 acataaaata atattttttg atattaagag ttgatttcaa aagagaaaac gtttactata
    22261 tagttattag atattaagac agtcaaggag tatcaaatga atttacccac tgaaatccgc
    22321 tatcgtcctt atgatgaatg gactgaagaa gataaggaaa atattgtaaa gaatgtctca
    22381 aaatcccctt ggagagcaac ttatcactta gaagctaaaa cagggctttt aaatgaccca
    22441 aatggcttct cttactttaa tggaaaattc catctttttt atcaaaattg gccctttgga
    22501 gctgcacatg gtttaaaaca atgggtacat acagaatcag atgatttagt tcatttcaaa
    22561 gaaacaggaa tcaagcttaa acctgaccat gtcaatgata gccatggtgc atactccggt
    22621 tctgcattag ccattgacga taagcttttt cttttttata ctggtaatgt ccgtgacatg
    22681 aaatggaacc gtgacccccg ccaaattgga gcatggatga caagtgatgg caagattaca
    22741 aaatttgata aagtccttat ttcacaacca aatgatgtca ctgagcattt tcgtgaccct
    22801 cagattttca attatgataa tcaattctat gctgttatcg gggcgcaaaa tagtaaaaaa
    22861 tgcggattca ttaaattata taaagcactt aataatgaca tccatcattg ggagtttgtg
    22921 ggagatttag attttggtgg tactggatct gagtatatga ttgaatgtcc aaatattatt
    22981 ttggtcaaag gaaaacctgt attactttat agtcctcaag gactagataa aaacgaattg
    23041 gactaccaaa acatttatcc aaatacctac aaaatcggac aatatttcga tgctaactca
    23101 tctaagatag ttgaaccatc ccctatctat aatttagatt atggttttga agcctatgct
    23161 actcaaggat ttaacacttc tgatggtcgt gcctttatcg tgagttggat aggcttgcca
    23221 gatattgatt atcctagcga tcaatttgat tatcaaggag ctatgagttt agttaaagag
    23281 ttaagcataa acaatgggaa cctttatcaa tatcccgttc ctgctatgaa aaatcttcgt
    23341 caacatcaag cagaatttaa aacacaactt caaactaaca acacctatga attagaatta
    23401 ctagttccta gaaacgatct ttcttcattt gttctctttg ctaatccgaa aggacaagga
    23461 ctatccatta ctattgatac tgcaaagggg aaagttatcg ttgaccgctc acaagccgga
    23521 cagcagtacg ccacagaatt tggtactagt cgccagtgtg acattccaaa agatgctact
    23581 agtgtcaata tttttgtcga taagtccatc tttgaaatct ttataaataa aggagaaaaa
    23641 gtatttacgg gacgagtctt tccagatgca gaacaatcag gaattcaact aaaagaagga
    23701 cacgttcatg gtaaatattt tgaattaaaa tattaatatg gttgcaaaat taacagatgt
    23761 tgctgcactt gccggtgtta gcccaacgac tgtatcacgc gttattaaca aaaaaggcta
    23821 tttatctcaa aaaacagtca ccaaagtcaa tgaagccatg agaacccttg gttataaacc
    23881 taataattta gcacgtagct tacagggaaa gtccgctaaa ttaattggac tcattttccc
    23941 caacatccgt aacatttttt acgctgaatt aattgaacac cttgaaattg agttgtttaa
    24001 gcatggttat aaaactatct tatgtaatag tgaaaaggat cctatcaaag aaaaagaata
    24061 cctcgaaatg ctaggagcaa atcaagtaga cggtatcatt tcaagtagtc acaacctagg
    24121 aattgatgat tacgagaaag ttgaggctcc catagtagca ttcgatagaa atttagctcc
    24181 acacatccct atcgtctcat ctgataattt ctttggagga aaaatggctg ctcaaaccct
    24241 taaaaaacat ggctgtcaaa agatgattat gattacaggg aatgataatt cagattctcc
    24301 tactggactt aggcggctag gtttttccta tgaatcaaag gaatccaaag ttatcacagt
    24361 cacaaatggg ctatctaaca tgagacgtga gatggaatta aaatcaatta tctcaactca
    24421 caaaccagat ggtattttta catcagacga cttaactgct ttattagtaa tcaaacttat
    24481 cagccaacta gggttgtcca ttccagaaga tatcaaagtg attggatatg atggtacaag
    24541 tttcattcaa gattacgtcc cacatctaac cactattaaa caacctattc gagaaattgc
    24601 gcaattgatg gttgaaatac tcctagctaa aattgagggg caaaagacaa ataaggatta
    24661 tattttacca gtcagtctca tccctggtag tagtgtttag gaaatcatat ttataaaata
    24721 atttttagta atagttattt ttcaaagcaa attaaaaatg tggaagaa
//
LOCUS       DATRNK010000025        23731 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.025, whole genome shotgun sequence.
ACCESSION   DATRNK010000025 DATRNK010000000
VERSION     DATRNK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23731
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.025"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            188..883
                     /locus_tag="VBC52_001022"
     CDS             188..883
                     /locus_tag="VBC52_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HEO6882619.1"
                     /translation="MNEVLRQEKKYLISLEDYYLLSKKFSQVMTLDSHSSDDGYTIRS
                     LYFDSLEDVDWQEKEDGVEIRRKIRLRNYGSQSNFAKLEMKQKQGANQKKRSLKLSKT
                     DAQALINGNNSVLLNYPDTFAAECYSRITTQLYQPKTVIEYQRKAFVAKENNIRITFD
                     HHIIGTEASYDIFSEDLNQNYLLDPYLVILEVKYNGFLLSYIKELLDSVDRSELSASK
                     YCLGRAVSKHYRF"
     gene            934..1611
                     /locus_tag="VBC52_001023"
     CDS             934..1611
                     /locus_tag="VBC52_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HEO6882620.1"
                     /translation="MKDLLRNSLEQSGNLSFQDMILHILVAALLSVVIYVSYAYTHSG
                     TAYSKKFNVSLMTLTVLTATVMTVIGNNVALSLGMVGALSVVRFRTAIKDSRDTVYIF
                     WTIVVGICCGVGDYVVAALGSSVIFILLWVMGRVKNENRMLLIVKCDRTLEVDLEGIF
                     FQYFDGKAVQRVKNSTTNTIEMIFEISRKDYDKQHHVDNQLTEKVYQLGNIDYFNIVS
                     QSDEING"
     gene            1604..3049
                     /locus_tag="VBC52_001024"
     CDS             1604..3049
                     /locus_tag="VBC52_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEO6882621.1"
                     /translation="MGRLMRGKVIYGTTLIGLFLFLFFYFWIPKHHIERIHHHRIKQV
                     DAKSDLTGFKTHLPIISIDTKQQAIPLVTKEGGKYVKARDNINADIELRDSPSRSHHL
                     SEKPRIRTKGLISYRGNSSRYFDKKSLKVKFVTNKLKEKKHRLAGMPKESEWVLHGPF
                     LDRTLLRNYLSYNIAGEIMSYAPNVRYCELFVNGEYQGVYLAVENIEQGEQRVPIEKS
                     DKKLHKTPYIVAWDREHKAKQKLDNYVHYTHQSGISALDVKYPGKQRLTSKQLEFINK
                     DINHIEKVLYSYDFSQYPKYIDRESFANYFVINEFFRNVDAGKFSTYLYKDLRDRAKL
                     VVWDFNNAFDNQIEGRVDEADFTLTDAPWFNMLIKDKAFIDLVVHRYKELRKGVLATE
                     YLSNYIDETRHFLGPAIDRNYKKWGYVFDLKNTDPRNYLIPTERNVTSYHKSVEQLKD
                     FIKKRGRWMDRNIETLYQYSAPSKNTNTLLE"
     gene            3056..5215
                     /locus_tag="VBC52_001025"
     CDS             3056..5215
                     /locus_tag="VBC52_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882622.1"
                     /translation="MKKYLSIVALLTILFSSIYYLYYFDGSLYLPKGLLKENTRTNFV
                     VKGDTVLHKPTNKPFVVKGVDVESSLAGYHHNDFPITQKTYREWFHLISNMGANTVRV
                     KVPMNVAFYDALYHHNKASKRPLYLLQGIRIDSYRNNASITAFNDNYRGYLKREAKGV
                     VDILHGRKQVWNTDFGSSHYHYDLSPWVLGYVVGDDWNSGTVAYTNHQEKKTQYKGRY
                     FKTSVAANPFEVMLAQVMDELTHYETAKYGWQHLISFSNSPTTDPFHYRKPFEAQAPK
                     YVQLNVENIQANSNVKAGMFAAYKAIDFHPRYKDYLLFDKENISKEDRQKIKELSLSQ
                     GYVKLLNAYHKIPVLVTGYGYSTARGIAQKEIDKRPLPINEKEQGQRLLEDYESFISS
                     GSFGATINAWQDDWNASVWNTSFATNKHSQFLWGDAQVFNQGYGLLGFKNAKHHYQVD
                     GKRGKEEWKHPLMTSATGDDLYASSDESYLYLAIKTKPEKLKEKRLLPIDITPKSGSR
                     KMNGSKVTFSKSSDFVLSIDPNGKSELFVQERYNALKANYLRQLNGKDFYAFPPKKNS
                     SNFEQINMVLRNTKIVEDMEKVKATERFLPTHPTGLLKTGTTDRHQKTFDSQTDISFG
                     KDFIEVRIPWQLLNFSDPSSQKIHDDYFKHYGVKELEIESIALGLGANSKENTLIKMA
                     DYRLKNWERPDTKTFLKDSYYSIKKEWSKERERTYGT"
     gene            5355..6767
                     /locus_tag="VBC52_001026"
     CDS             5355..6767
                     /locus_tag="VBC52_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6882623.1"
                     /translation="MALFFTFLKVSQVIFLVLLIAYASFLVISLVIGGVHLYEKNERK
                     KSLTRNKEKIPISIIVPAYNESTTIVSSIDSLLHLDYEAYEIIVVDDGSSDNTSDVLK
                     EEFALMKISNTIDSIIATQTCKDVFQRQVGKVKLTLIVKENGGKGDALNMGINAANYD
                     YFLCLDADSMLQVDSLSQISKSIQVDPTVIAVGGLVQVAQGVKIEQGKVASYRLPWRI
                     IPCAQALEYDSSFLGARIFLDYLRANLIISGAFGLFKKDLVKAVGGYDTQTLGEDMEL
                     VMKLHFFCRNNNIPYRICYETDAVCWSQAPTNLGDLRKQRRRWYLGLYQCLKKYKSIF
                     ANYRFGAVGSISYIYYILFELLTPFIECFGIVIIFLSLLFNQLNIPFFISLVSLYIFY
                     CVLITLSSFLHRIYSQQLVIGILDIVKVFYIAVFRYLILHPVLTFVKVASVIGYKNKK
                     MVWGHITREKQMKDFETSVT"
     gene            7013..7546
                     /locus_tag="VBC52_001027"
     CDS             7013..7546
                     /locus_tag="VBC52_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6882624.1"
                     /translation="MACIIRRARLGDEVNLAYIQTESWKAAFGKILPEDIIQKTTEIE
                     PAITMYQQLLHKEIGKGYILEVDSNPHCMAWWDKSREDDMLDYAELICIHSLKEGWGK
                     GYGSQMMNHVLSEIQQAGYNKVTLWVFTENTRARKFYDRFGFSFKGKSKTYFEKEEMC
                     YEKILHQRDKASDNTIV"
     gene            7782..8030
                     /locus_tag="VBC52_001028"
     CDS             7782..8030
                     /locus_tag="VBC52_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882625.1"
                     /translation="MEKIMNDFEKVIAVLNQLSISYELVENGPVLLTEQADNFIVGIE
                     GVRTKIMFLTNKKKMKCYLLIMDDQKLFYPMKTIVAFR"
     gene            8120..8356
                     /locus_tag="VBC52_001029"
     CDS             8120..8356
                     /locus_tag="VBC52_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEO6882626.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTLEVNGKVLQAT
                     PYFLINVTGEGEGFSMWAPTPCDVLAEDWIEVND"
     gene            8349..9047
                     /locus_tag="VBC52_001030"
     CDS             8349..9047
                     /locus_tag="VBC52_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEO6882627.1"
                     /translation="MTKVVLVTGCASGIGYAQAQYFLKQGYQVYGVDKLDKPNLNGNF
                     NFIKLDLSSDLSPLFTMVPSVDILCNTAGILDAYKPLLEVSDEEVEHLFDINFFVTVR
                     LTRHYLRRMVEKKSGIIINMCSIASFIAGGGGAAYTSSKHALAGFTRQLALDYAKDCI
                     QIFGIAPGAVQTAMTASDFEPGGLAEWVASETPIGRWTKPSEVAELTGFLASGKARSM
                     QGEIVKIDGGWSLK"
     gene            9044..9289
                     /locus_tag="VBC52_001031"
                     /pseudo
     CDS             9044..9289
                     /locus_tag="VBC52_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233822.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3977 family protein"
     gene            9286..9738
                     /locus_tag="VBC52_001032"
     CDS             9286..9738
                     /locus_tag="VBC52_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEO6882628.1"
                     /translation="MKAQELWNKFKSLNPSIGDEIDAWQFGAEPDLLAKLVVDGTKTA
                     TASAYDLYELDEEELPQVGSYDIILDSNDEAICIIEITKVDIVPFKDVSADHAFKEGE
                     GDKTLEWWRRAHIDFFKPYFEEFGLMFSEDSRIVLEEFQVVYPKEINE"
     gene            9735..10691
                     /locus_tag="VBC52_001033"
     CDS             9735..10691
                     /locus_tag="VBC52_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6882629.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIDGIDLVAILSTVRSLETAKDLA
                     KEYNMSLATSEYKAVLDNEEIDTVYIGLPNHLHFDYAKEALLAGKHVICEKPFTLEAS
                     QLEELVSIANTRQLILLEAITNQYLPNFDLVKEHLSNLGDIKIVECNYSQYSSRYDAF
                     KRGEVAPAFNPEMGGGALRDLNIYNLHLVIGLFGEPITAQYLPNIERGIDTSGVLVLD
                     YGHFKTVCIGAKDCSAEVKSTIQGDKGSIAILGPTNTMPKISLTMNGQESHVYQLNGD
                     RHRMHDEFVIFEGIISNLDFKRAAQALEHSRTVMKVLDMVTK"
     gene            10780..11157
                     /locus_tag="VBC52_001034"
     CDS             10780..11157
                     /locus_tag="VBC52_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6882630.1"
                     /translation="MISSIGQVMLYVSNVEASADFWKNKVGFERVEKQTQGDYVTYIV
                     APKLDSEVSFVLHDKAIIAQMSPELDLATPSILFETTDIDSTYQELTANEVMTNPIVD
                     MGSMRVFNFSDNDNNYFAIREVK"
     gene            11170..11583
                     /locus_tag="VBC52_001035"
     CDS             11170..11583
                     /locus_tag="VBC52_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6882631.1"
                     /translation="MVKALETYIVTNGNGRQAIDFYKNVFQADLVNMMTWEEMDPNCL
                     EDRKDLIINAQLIFDGIRLQISDENPDFVYQAGKNVTAAIIVGSVEEAREIYEKLKKN
                     AQEVQLELQETFWSPAYANLVDQFGVMWQISTELN"
     gene            11827..13206
                     /gene="glmU"
                     /locus_tag="VBC52_001036"
     CDS             11827..13206
                     /gene="glmU"
                     /locus_tag="VBC52_001036"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEO6882632.1"
                     /translation="MSNYAIILAAGKGTRMKSDLPKVMHKVSGITMLEHVFRSVQAIE
                     PSKIVTVIGHKAELVRDVLGDKSEFVMQTEQLGTGHAVMMAEEELATSKGHTLVIAGD
                     TPLITGESLKNLIDFHVNHKNVATILTADAANPFGYGRIIRNSDDEVTKIVEQKDAND
                     FEQQVKEINTGTYVFDNQSLFEALKDINTNNAQGEYYLTDVIGIFKEAGKKVGAYKLR
                     DFDESLGVNDRVALATAEKVMRQRIARQHMVNGVTVVNPDSAYIDIDVEIGEESVIEP
                     NVTLKGQTKIGKGTLLTNGSYLVDAQVGNDVTITNSMVEESIISDGVTVGPYAHIRPG
                     TSLAKGVHIGNFVEVKGSQIGENTKAGHLTYIGNAEVGCDVNFGAGTITVNYDGQNKF
                     KTEIGSNVFIGSNSTLIAPLEIGDNALTAAGSTITDNVPIDSIAIGRGRQVNKEGYAN
                     KKPHHPSQK"
     gene            13227..13781
                     /locus_tag="VBC52_001037"
     CDS             13227..13781
                     /locus_tag="VBC52_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6882633.1"
                     /translation="MDFEEKTINRQTVFDGQIIKVAVDDVELPNGLGQSKRELVFHGG
                     AVATLAVTPEHKIVLVKQYRKAIEGISYEIPAGKLETGESGSKEEAALRELEEETGYT
                     GNLEILYSFYTAIGFCNEKIVLYLATDLQKVENPRPQDDDEVLELLELSYEDCMQMVE
                     KGMIQDAKTIIALQYYGLKMGGHS"
     gene            13781..14050
                     /locus_tag="VBC52_001038"
     CDS             13781..14050
                     /locus_tag="VBC52_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882634.1"
                     /translation="MGKPLLTDDMIERSNRGEKVSGQTILDQETKIISTEDGMEQLTD
                     ENGKHIYKSRRIENAKRNEFQRKLNLVLFILLILLALLFYAIFKL"
     gene            14060..14749
                     /locus_tag="VBC52_001039"
     CDS             14060..14749
                     /locus_tag="VBC52_001039"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEO6882635.1"
                     /translation="MKIGIIAAMEEELKLLVENLEDKSQETVLSNVYYSGRYGEHELV
                     LVQSGVGKVMSAMSVAILVESFKVDAIINTGSAGAVATGLNVGDVVVADTLVYHDVDL
                     TAFGYDYGQMSMQPLYFHSDKTFVSTFEAVLSKEEMTSKIGLIATGDSFIAGQEKIDV
                     IKGHFPQVLAVEMEGAAIAQAAQATGKPFVVVRAMSDTAAHDANITFDEFIIEAGKRS
                     AQVLMAFLKAL"
     gene            complement(14789..15436)
                     /locus_tag="VBC52_001040"
     CDS             complement(14789..15436)
                     /locus_tag="VBC52_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEO6882636.1"
                     /translation="MTPNKEDYLKCIHELGEKNNKISNKKIAEMMQVSAPAVSEMIKK
                     MISEQLIVKDKDLGYYLTKQGLLVVSDLYRKHRLVEVFLVNHLHYTADDIHKEAEVLE
                     HTVSTTFVDQLEKLLDFPQFCPHGGTIPKKGEFLVEINQMTLDQISQLGTYVISRVHD
                     DFQLLKYLEQHRLHINDTIELTQIDPYAKTYHIIYNDENLTIPERIASQIYVSAT"
     gene            15603..16529
                     /locus_tag="VBC52_001041"
     CDS             15603..16529
                     /locus_tag="VBC52_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEO6882637.1"
                     /translation="MKKWLVIVSCFVAFLGLGACANKQDQAKNEKLKVVVTNSILADI
                     TKNIAGNKIDLHSIVPVGKDPHEYEPLPADVEKTTQADLVFYNGINLETGGNAWFTKL
                     IKNAKKKENKDYFAVSDGVDVIYLNGQSGKGKEDPHAWLNLENGIIYSKNIAKQLIAK
                     DPKNKATYEKNRDAYVAKLEKLDKEAKSKFNAIPANKKLIVTSEGCFKYFSKAYGVPS
                     AYIWEINTEEEGTPDQITSLVKKLKQVRPSALFVESSVDKRPMKSVSRESGIPIYAEI
                     FTDSIAKKGQKGDSYYAMMKWNLDKIAEGLAK"
     gene            16700..17416
                     /locus_tag="VBC52_001042"
     CDS             16700..17416
                     /locus_tag="VBC52_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO6882638.1"
                     /translation="MIISKHLSVSYDNNLVLEDINLRLEGSGIIGILGPNGAGKSTLM
                     KALLGLVDSTGESGIGGDLLPLMGRVAYVEQKTNIDYQFPITVGECVSLGLYKERGLF
                     KRLSKTDWEKVSRVIDQVGLRGFENRPINALSGGQFQRMLMARCLVQEADYIFLDEPF
                     VGIDSISEQIIVNLLKKLSKAGKLILVVHHDLSKVDHYFDQVIILNRHLIACGPIDQA
                     FTRENLSAAYGDAILLGQGD"
     gene            17418..18251
                     /locus_tag="VBC52_001043"
     CDS             17418..18251
                     /locus_tag="VBC52_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO6882639.1"
                     /translation="MFTKFFEGLLTYHFLQNAFITAIVIGIVAGAVGCFIILRSMSLM
                     GDAISHAVLPGVAISFILGINFFIGAIVFGLLSSIIITYIKENSVIKGDTAIGITFSS
                     FLALGIILIGLANSTTDLFHILFGNILAVQDSDKYMTIIVGLIVLTLITIFFKELLLT
                     SFDPVLAKSMGMRVSFYHYLLMILLTLVAVTAMQSVGTILIVALLITPAATAYLYVKS
                     LRTMLFLSSALGAVASVLGLYIGYTFNIAAGSSIVLTSTFMFLLAFLFSPKQSLFKKK
                     Q"
     gene            complement(18303..19106)
                     /locus_tag="VBC52_001044"
     CDS             complement(18303..19106)
                     /locus_tag="VBC52_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEO6882640.1"
                     /translation="MKKIIYLGLACVSILTLSGCESIERSLKGDRYVDQKLAENSSKE
                     ATEQLNKKTKQALKADKKAFPQLDKAVAKNEAQVLIKTSKGDINIKLFPKYAPLAVEN
                     FLTHAKEGYYNGLSFHRVIKDFMIQSGDPNGDGTGGKSIWNSKDKKKDSGNGFVNEIS
                     PYLYNIRGSLAMANAGADTNGSQFFINQSQQDHSKQLSDKKVPKVIIKAYSEGGNPSL
                     DGGYTVFGQVISGMETVDKIASVEVTKSDQPKEKITITSIKVIKDYKFK"
     gene            19272..21722
                     /locus_tag="VBC52_001045"
     CDS             19272..21722
                     /locus_tag="VBC52_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO6882641.1"
                     /translation="MVFMANKKKTKGKKTRRPTKAEIERQRAIQRMITALVLTIILFF
                     GIIRLGIFGITVYNVIRFMVGSLAYLFIAATLIYLYFFKWLRKKDSLVAGFLIASLGL
                     LIEWHAYLFSMPILKDKEILRSTARLIVSDLMQFKITVFAGGGMLGALIYKPIAFLFS
                     NIGAYMIGVLFIILGLFLMSSLEVYDIVEFIRAFKNKVAEKHEQNKKERFAKREMKKA
                     IAEQERIERQKAEEEAYLASVNVDPETGEILEDQAEDNLDDALPPEVSETSTPVFEPE
                     ILAYETSPQNDPLPVEPTIYLEDYDSPIPNMRENDEEMVYDLDDDVDDSDIENVDFTP
                     KTTLVYKLPTIDLFAPDKPKNQSKEKDLVRKNIRVLEETFRSFGIDVKVERAEIGPSV
                     TKYEIKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSF
                     RELWEQSDANPENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISS
                     ILMKARPDQVKFMMIDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYE
                     LFSKIGVRNIAGYNTKVEEFNASSEQKQIPLPLIVVIVDELADLMMVASKEVEDAIIR
                     LGQKARAAGIHMILATQRPSVDVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKL
                     LGRGDMLFKPIDENHPVRLQGSFISDDDVERIVGFIKDQAEADYDDAFDPGEVSETDN
                     GSGGGGGVPESDPLFEEAKGLVLETQKASASMIQRRLSVGFNRATRLMEELEAAGVIG
                     PAEGTKPRKVLMTPTPSE"
     gene            21804..23519
                     /gene="pabB"
                     /locus_tag="VBC52_001046"
     CDS             21804..23519
                     /gene="pabB"
                     /locus_tag="VBC52_001046"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEO6882642.1"
                     /translation="MHIETVIDFKELGKRYRFKNPTKELIADTLEQVLEVIKEVDYYQ
                     SQNYYVVGYLSYEASAAFDSHFKVSQQKLAGEHLAYFTVHKDCENEAFPLSYENVRLA
                     DNWTANVSEQEYQEAIANIKGQIRQGNTYQVNYTLELSQQLCSDPFSVYERLMVEQGA
                     GYNAYIAYDDKRILSVSPELFFKKKDEVLTTRPMKGTSARKPTYQEDVAERDWLANDP
                     KNRSENMMIVDLLRNDMGRICDVGTVKVKKLCQVEQYATVWQMTSTIEGVLSPEVTLM
                     SIFQALYPCGSITGAPKISTMAIINELEKRPRGIYCGTIGLCMPDGQAIFNVPIRTVQ
                     MKGQQAYYGVGGGITWESQTDSEYEETRQKSAVLTRVNPKFQLITTGRVTENKLLFSQ
                     QHVERLVESASYFAYSFDKSKFERELKKYLHQLDEKDYRLKIMLDKTGKVTFEVKQLV
                     NLSKKFLTAEVVVQDYPIKLSPFTYFKTSYRPHIIEGQNEKIFVSPEGLLLETSIGNI
                     VLEKNGRFLTPDLSEGGLNGIYRRHLLENQKVIEAPLTLKDLESADAVYACNAVRGLY
                     PLNLK"
     gene            complement(23548..>23731)
                     /locus_tag="VBC52_001047"
     CDS             complement(23548..>23731)
                     /locus_tag="VBC52_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3397 family protein"
                     /protein_id="HEO6882643.1"
                     /translation="LLPYYLILISLLAIGITIKLLRTKTLFSYKRFLKLFWRSGFILT
                     FLFYLGLLVIIFIKVQ"
BASE COUNT         7979 a         3389 c         4743 g         7620 t
ORIGIN      
        1 aggtttaaag gagcgtaaca atcatattta tagtataaaa ataaagttta aaatgccttc
       61 ttataaaaaa atatacatta tatattttcc tgttgacaag gtgtataatt tctgatatat
      121 ttatttttgc ttaatttatt gttaatattt atatataaat taatgtttaa ttaaatagag
      181 gtgagtgatg aacgaagttc ttagacagga aaaaaaatat ttaatatcac ttgaagatta
      241 ctatctttta tctaagaaat tttcccaagt aatgacgctc gattcgcata gttcagacga
      301 tggctatact atccgttcgc tatactttga ttctttagaa gatgttgatt ggcaagaaaa
      361 ggaagatggt gttgagatac gaagaaagat tcgtttgcgc aattatggta gtcaaagcaa
      421 ttttgcgaaa ttagaaatga aacaaaaaca aggtgctaat caaaaaaagc gctctctaaa
      481 attaagcaag acagatgctc aagcgctgat taatggtaac aatagtgttt tattaaatta
      541 tccggataca tttgctgcgg agtgttattc aaggataaca acgcaacttt atcaacctaa
      601 aacggttatt gagtaccaaa gaaaagcttt tgttgctaag gaaaacaata tccgaatcac
      661 ctttgaccac catataatag gtacagaggc tagttatgat attttctcag aggatttaaa
      721 tcaaaattat ttattagatc cttaccttgt tattttagaa gttaaatata atggattttt
      781 attgtcttat attaaagaac tcttagattc ggtagataga agtgaacttt cagcgagtaa
      841 gtattgttta ggaagagctg ttagtaaaca ttatcgtttt tagtgaaaaa gtatatacgt
      901 taattaatat taaatgtgag aggataacaa aaaatgaaag acctattacg aaatagtcta
      961 gagcaaagtg gaaatttaag ttttcaagat atgattttac atattcttgt agcagcttta
     1021 ttgagtgtag ttatttatgt ttcctatgct tatacgcata gtggaactgc ctatagtaaa
     1081 aagtttaatg tttcattaat gacattgacg gtcttgactg caacagtaat gaccgttatt
     1141 ggtaataatg tagccttgtc attgggtatg gtcggtgcct tgtcagttgt tcgttttagg
     1201 acagccataa aagattcaag agatacagtt tatatttttt ggaccatagt tgttggtatc
     1261 tgttgtggtg tcggtgacta tgtggtagcg gcattaggaa gtagcgttat ctttatctta
     1321 ctatgggtta tgggacgtgt taaaaacgag aatcgtatgt tattgattgt gaagtgcgat
     1381 agaacactag aagttgattt agaaggaatt ttcttccaat attttgacgg aaaagctgtt
     1441 cagcgtgtta aaaattcaac aactaatact attgaaatga ttttcgaaat ctctagaaaa
     1501 gattacgata agcaacacca tgtagataat cagttaactg aaaaagtgta ccaattggga
     1561 aatattgatt atttcaacat tgttagccaa agcgacgaaa tcaatgggta gacttatgag
     1621 agggaaggtt atttacggca caacccttat aggtcttttt ctattcttat ttttctattt
     1681 ttggattcct aagcatcaca tcgaaagaat acatcatcat cgtataaagc aggtagatgc
     1741 gaagagtgat ttaacaggat ttaaaaccca tttgcccatt atcagcatcg atacaaagca
     1801 acaagctatt cctcttgtta caaaagaagg tggaaaatat gtcaaagcta gggataatat
     1861 taatgctgat atcgaattac gggattctcc aagtagatca catcatttat cagaaaagcc
     1921 gagaattagg acaaaagggt taatatcata tagaggaaat tcctctcgtt actttgataa
     1981 gaagtcactg aaagttaagt ttgtcactaa taagttaaag gaaaagaagc atcgattagc
     2041 aggaatgcct aaagaatcgg agtgggtatt gcatggtccc tttctagaca gaacattatt
     2101 aagaaattat ctgagttata atattgctgg tgagattatg tcctatgccc caaacgttcg
     2161 ctactgtgag ttatttgtca atggtgagta tcagggagta tatctagcag ttgagaatat
     2221 cgaacaaggt gagcaacgag ttcccattga aaaaagtgat aaaaagttgc ataaaacacc
     2281 ttatatcgtt gcttgggatc gtgaacataa ggcaaagcaa aagttagata actatgtaca
     2341 ttatacacac cagtctggta tctcagctct tgatgtgaaa tatccaggta aacaaagatt
     2401 gacatcaaaa caattagagt ttattaataa ggacatcaat catattgaaa aagtccttta
     2461 ttcttatgat ttttcacaat atcctaaata tattgataga gaatcatttg caaattattt
     2521 tgtcatcaat gaatttttta gaaatgtgga tgcgggtaaa ttttcaacct atctatataa
     2581 agatcttcgt gatagagcaa aactagtagt ttgggatttt aataatgctt ttgataatca
     2641 aattgaagga cgtgttgatg aggcagattt taccttgacg gatgcaccgt ggttcaatat
     2701 gctcattaag gacaaagcat ttatagacct ggttgttcat cgctataaag aattacgaaa
     2761 gggagttcta gcgacagaat atttatctaa ttacatcgac gagacaaggc attttttagg
     2821 tccagctatt gatcgaaatt ataaaaaatg gggctatgtc tttgatttaa agaatactga
     2881 tccaaggaat tatttgattc ctactgaacg caatgtgaca agttatcaca aatcagtaga
     2941 acaattaaaa gattttatca aaaagcgtgg cagatggatg gatagaaata ttgagacctt
     3001 gtatcaatat agtgcaccat cgaaaaatac taatacttta ctggagtaga acattatgaa
     3061 aaaatatcta agtattgtag cccttttaac tattttgttt agctctattt attatttgta
     3121 ttactttgat ggtagtttgt atttaccaaa gggcttatta aaagaaaata caagaactaa
     3181 ctttgttgtt aaaggtgata ctgtacttca caagcccacc aataaacctt ttgttgttaa
     3241 aggagtagac gttgagtctt ccttagcggg ttatcatcac aacgattttc ctattactca
     3301 aaaaacgtat cgtgaatggt tccatttaat ttccaacatg ggggcaaata ctgtaagagt
     3361 caaggtaccg atgaatgttg cattttacga tgccttatat caccacaaca aagcatcaaa
     3421 gaggccactg tatttgttgc aaggaatacg tatagattct tatcgcaata atgcttctat
     3481 aacagctttt aatgataatt atagggggta tttaaaacga gaagcaaaag gcgttgtgga
     3541 tattctccat gggcgtaagc aagtatggaa tactgatttt ggtagcagtc attatcatta
     3601 tgatcttagt ccttgggtac ttggttatgt cgtaggggat gattggaata gtggtactgt
     3661 cgcttatact aatcatcaag agaaaaaaac gcaatataaa ggacgttatt ttaaaacttc
     3721 tgtggcagct aatccatttg aggtcatgct agctcaagta atggatgaat tgacacatta
     3781 tgagacagct aaatatggtt ggcaacattt gattagtttt tcaaactcac caacaacaga
     3841 cccttttcat tatcgaaaac catttgaggc acaggctcct aaatacgtac aactaaatgt
     3901 agaaaatatt caagctaatt cgaatgttaa agcaggtatg tttgcagcat ataaagctat
     3961 tgatttccat cctcgataca aggattatct attatttgat aaagagaata tcagtaaaga
     4021 agatagacaa aagattaaag aactttcttt gtcacaggga tacgttaaac tgctaaatgc
     4081 ttatcacaaa atccctgttc tagtcacggg ttatggctac tcgacagcga gaggtattgc
     4141 ccaaaaagaa attgataaac gtcctctgcc gattaatgaa aaagaacaag gtcagcgttt
     4201 actagaagat tatgaatctt ttatatcatc cggtagtttt ggagcgacta tcaatgcatg
     4261 gcaagacgat tggaatgcaa gtgtgtggaa tacatccttc gccacaaata aacatagtca
     4321 attcctatgg ggggatgcac aagtatttaa tcaaggttat ggtttattag gctttaaaaa
     4381 cgcaaaacat cattatcagg ttgatggtaa aagaggcaaa gaagagtgga aacatcctct
     4441 gatgactagt gcaacaggag atgacttata tgctagcagt gatgaaagct atctctacct
     4501 tgcgattaaa acaaaacctg aaaaactaaa agaaaaacga ttattaccaa tagatattac
     4561 accaaaatct ggtagtagaa aaatgaatgg tagtaaggtc acattttcta aatctagtga
     4621 ctttgtattg tctattgatc caaatggcaa gtctgaatta tttgtccaag agcgctataa
     4681 cgccttaaaa gcgaactatc ttcgacagct taatggtaaa gatttttatg ctttcccacc
     4741 aaagaagaac agtagtaatt ttgagcagat aaatatggta ttgagaaata caaagattgt
     4801 tgaagacatg gaaaaagtaa aagcaacaga gaggttctta ccaactcatc ctactggtct
     4861 tctcaaaaca ggaacaactg ataggcacca aaaaacattt gattcacaaa cagatatttc
     4921 gtttggaaag gactttatag aggtcagaat tccgtggcag ttgttgaatt tttctgatcc
     4981 atcatctcaa aaaattcacg atgattactt taaacattat ggtgtgaagg agttagaaat
     5041 tgagagcatt gctttaggat taggtgctaa tagcaaagaa aacacactga taaagatggc
     5101 agattatcgt ttgaaaaatt gggagagacc cgataccaaa acctttttaa aagactccta
     5161 ttatagtatt aagaaagaat ggtctaaaga aagagagaga acatatggta cataacattt
     5221 tatattttta cattttgatt tgcttagcaa ttattgtttt taacattcac tatatcttaa
     5281 gacaacgatt agataagctt agttggcttc gtattcggaa aaaggatata aatatcaaat
     5341 aaaggggatt ttctatggca ttatttttta ccttcctaaa ggtatctcaa gtcattttct
     5401 tagtattatt gattgcctat gccagctttt tagtgatttc attagtcatt ggaggtgttc
     5461 atctctatga aaaaaatgag cgcaaaaaaa gtttaactag aaataaagaa aaaattccaa
     5521 tttcaattat tgtaccagcc tataatgaat caacaaccat tgtgagttcg attgattcgt
     5581 tattgcacct tgattatgaa gcatatgaaa ttatcgttgt ggatgacggt tccagtgaca
     5641 atacttcaga tgttctcaag gaagagtttg cattaatgaa aatctcgaat acgattgatt
     5701 caattattgc tactcaaact tgtaaagatg tctttcagag acaggttggt aaggttaaac
     5761 tgacactcat tgttaaagaa aatggtggca aaggggatgc cttaaatatg gggatcaatg
     5821 ctgctaatta cgattatttt ctatgtctag atgcagattc tatgttgcag gtagattctt
     5881 tgagtcaaat tagcaaatcc atacaggtag atccgacagt tatagcagtg ggagggctcg
     5941 tccaagttgc ccaaggtgtt aagattgagc aggggaaagt agcatcttat cgtttgcctt
     6001 ggagaattat tccttgtgca caagcacttg agtatgattc atcattttta ggagctcgaa
     6061 tctttttaga ctatttaaga gctaatttaa ttatttcggg ggctttcggt ctttttaaga
     6121 aagaccttgt taaagcggtg ggaggatatg acacccagac tctaggagaa gatatggagt
     6181 tggtgatgaa attacatttc ttctgtcgta ataataatat tccctatcgt atttgttatg
     6241 aaacagatgc tgtttgttgg agccaggcac caacgaattt aggggattta cgtaaacaga
     6301 gacgccgatg gtatcttggc ttgtaccaat gtttaaaaaa atataaaagt atttttgcca
     6361 attatcgctt tggagctgta ggatcaattt cttatattta ctatatttta tttgaattac
     6421 tgaccccgtt tatagaatgt tttggtatcg tcattatatt tttgtcctta ttatttaatc
     6481 aattaaacat cccattcttt atatcattgg tgagccttta tatcttttac tgtgttttaa
     6541 ttacattaag ctctttctta caccgtattt attctcagca actagtaata ggaattttag
     6601 atattgttaa agtattttat attgctgttt ttaggtatct tatcctacat cctgtattaa
     6661 cttttgttaa ggttgcaagt gttattggct ataaaaataa aaaaatggtt tgggggcata
     6721 ttacgagaga gaaacaaatg aaggattttg aaacaagcgt tacttagaag ttatagcgat
     6781 tcatatatat atatataaaa taatcactaa aagagcgtag ttatctacgc tcttttagtg
     6841 attatcaata attggcctaa tctgaggtta atgtttaaaa ccatctaaaa ttttagaaat
     6901 ataatgataa atgagaacca gaactgttca aaataaaaat taatattacg aaaatggtat
     6961 aattggagta gttatcagac agttaataat catttttgaa aggattttag ttatggcttg
     7021 cataatcagg agagcaagat taggagatga agtcaattta gcttatattc aaacggagag
     7081 ctggaaagct gcttttggta agatactacc tgaggatatc attcaaaaaa ctacggaaat
     7141 agaacctgcc ataacaatgt atcaacagct actacacaag gagattggta agggttatat
     7201 attggaagta gatagtaatc ctcattgtat ggcttggtgg gataaaagta gagaagatga
     7261 tatgcttgat tatgcagaac tcatttgtat acatagtctt aaagaaggtt ggggaaaagg
     7321 ttatgggtct caaatgatga atcatgtact atcagagata caacaagcag gttataataa
     7381 agtgacgctt tgggtattta cagaaaatac acgcgcaaga aaattttacg ataggtttgg
     7441 tttttctttt aagggtaaat caaaaactta ttttgaaaag gaagaaatgt gttatgagaa
     7501 gattttgcac cagcgagata aagcatccga taacacaata gtataaacat cctattttga
     7561 aaaacaattt cctttaagag gaacaaattt gtaaaagaaa attgttatag gtataatgaa
     7621 gattatgaag gtaaaatttt ttgagtgaaa aacagtgtat caattttagg ttctctagtc
     7681 tacaaaaaga taattagatt tactgtttca ttttataaaa aattcaccca acaagtttca
     7741 gaaatcattt acacaaattg ttatgctaac tatatggaag aatggagaaa attatgaatg
     7801 attttgaaaa agttatagct gtgctaaatc aactaagtat ttcttatgaa ttagtggaaa
     7861 atggacctgt tttgttgacg gagcaagcag ataactttat tgtgggaatt gaaggagtaa
     7921 gaacaaaaat aatgttcttg accaacaaga aaaagatgaa atgttattta ctaataatgg
     7981 atgaccagaa actattttat cctatgaaaa caatagtagc ttttagatga actaaaaggc
     8041 gaagaaactt aaaattatga ttttaaataa gcaataaacc ttgatatatc aatggttaaa
     8101 gacaataaag gagaacaata tgacatttga agaaatttta ccaggtttaa aagcaaagaa
     8161 aaaatatgta cgtacaggtt gggggggtgc tgaaaattat gttcagttat ttgataccct
     8221 tgaagttaat gggaaagtac tacaggctac accttatttt cttattaatg ttactggtga
     8281 aggtgaaggt ttttccatgt gggcaccaac accatgtgat gttttagcag aggattggat
     8341 cgaggtcaat gactaaagtt gttttagtga cgggctgtgc tagtggaatt ggttatgcac
     8401 aggctcaata ttttttgaaa caagggtatc aagtatatgg tgtggataag ttggataaac
     8461 cgaacttaaa cggtaatttt aactttataa agttggattt atcctctgat ttatccccac
     8521 tattcacaat ggtaccgagt gttgatattt tgtgtaatac tgcaggtatt ctagatgcct
     8581 acaagccact tctagaagta agtgatgaag aagtggaaca cctatttgat attaattttt
     8641 ttgtgactgt taggctaaca cgtcattatc ttagacgtat ggtggaaaag aagtcaggta
     8701 ttattatcaa tatgtgctca atagcaagtt ttatagcggg tggcggtgga gctgcatata
     8761 ctagttctaa acatgccctt gcgggattca cacgtcaatt ggctttggat tacgcaaaag
     8821 attgtattca aatttttggt atagcgccag gtgctgtaca aactgctatg actgctagcg
     8881 attttgaacc tggtggatta gcagaatggg tagctagtga gacgccaatt ggtcgttgga
     8941 ctaaaccaag tgaagttgct gaattaactg gatttcttgc ttcaggaaaa gcgagatcaa
     9001 tgcaaggtga aattgtcaaa attgatggtg gctggagttt gaaatgagaa aatagattga
     9061 atttggttat ggtaatactt ggttattacg tacggaattt gaagcatctg atggtactga
     9121 gtgggaagtt aaaggaatat caggtgatat tttaataaaa tctgtttatt tacgtgtttg
     9181 gattggtcaa actgtttata ttttagatgt taaagaagga tttaaaagac aaagaaaaat
     9241 gagtaacgca gttaagtttg ttgtgggaat tgcttcggag gtaatatgaa ggcacaagaa
     9301 ttatggaata agtttaaatc tttgaatcct tcaatcggtg atgagataga tgcatggcaa
     9361 tttggagcag aaccagatct tctggcaaag ttagttgtag atgggacaaa aactgcaacg
     9421 gcctctgcct atgatttata tgaattagat gaggaagagt taccacaagt tggttcatat
     9481 gatattattt tggatagcaa tgatgaagct atctgtatta ttgaaataac taaagttgat
     9541 attgtccctt ttaaagatgt ctctgcagac catgctttta aagaaggaga aggagataaa
     9601 acattagaat ggtggagaag agctcacatt gatttcttta aaccttattt tgaggagttt
     9661 ggtttaatgt ttagtgagga ctctaggata gttttagagg aattccaagt ggtttaccca
     9721 aaggagatta acgaatgaaa ttagcggttt tggggacagg tatgattgtt aaggaagttt
     9781 tgcctgtctt gcagaaaatt gatggtattg atttagttgc tatcttatca acagttagaa
     9841 gtttggagac agctaaagat ctagctaagg aatataacat gtctctagct acgagtgaat
     9901 ataaggccgt cttggacaat gaggagattg atacagttta tattggactg ccgaatcact
     9961 tgcattttga ctatgctaag gaggcactct tggcaggaaa acatgtcatt tgtgagaagc
    10021 catttacatt agaagcaagt caattagaag aacttgtatc tattgccaat actaggcaat
    10081 taatattact cgaagcaatt acaaatcaat acttacctaa ttttgatctt gtaaaagaac
    10141 atttaagcaa ccttggtgat attaaaattg tagaatgtaa ctattcccaa tattcatcgc
    10201 gttatgatgc ctttaaacgt ggggaagtag caccagcttt caatcctgaa atgggtggag
    10261 gtgcgttgag agaccttaat atttataatt tacatttagt aattggactt tttggagaac
    10321 ctataacagc acagtattta ccaaatatag agcgtggtat tgatacttca ggagttctag
    10381 tcctagacta tgggcacttt aaaacagtct gtattggagc aaaagattgt agtgcagaag
    10441 taaaatctac tattcaaggt gataaggggt ctatagcgat tttagggcca actaatacta
    10501 tgcctaaaat ttcattaact atgaatggac aagaaagtca tgtttatcaa ttaaatggtg
    10561 ataggcaccg catgcacgat gaatttgtta tttttgaagg tattattagc aatttagatt
    10621 ttaagcgtgc tgctcaagct ttagaacata gtagaacggt gatgaaagtt ctagatatgg
    10681 taacgaaata agtaaatgac tcttttgagt cattttttac ttattttaaa caccgtatgt
    10741 tatactaatt agtaataaaa ccgaaagtga gataataaaa tgatttcatc aattggacag
    10801 gtaatgctct acgttagcaa tgttgaagca tcagcagatt tttggaaaaa caaggtgggt
    10861 tttgagcgtg ttgaaaaaca aacacaagga gattatgtaa cctatatagt tgcaccgaag
    10921 ttggatagtg aagttagctt cgtcttacat gacaaagcaa ttatagctca aatgagtcct
    10981 gagctagatt tagcaactcc ctctatttta tttgaaacta ctgatattga cagtacatat
    11041 caagaattaa cagcaaatga ggttatgaca aatcctattg ttgatatggg gagtatgaga
    11101 gtttttaatt tttccgataa tgacaataat tactttgcaa tccgtgaagt aaaataaaaa
    11161 ggagaactta tggttaaagc actagaaaca tacatcgtaa caaatggaaa tgggcgacaa
    11221 gcgattgatt tttataaaaa tgtctttcaa gctgatttag taaatatgat gacatgggaa
    11281 gaaatggacc ccaactgtct tgaagaccgc aaggatttaa tcattaatgc tcagcttatt
    11341 tttgatggca ttcgtcttca aatttcggat gaaaatccag atttcgtgta tcaagctggt
    11401 aaaaatgtta cggccgctat tattgtgggt agcgtagagg aggcgcgtga aatttatgaa
    11461 aaattaaaga agaatgcaca agaagttcag ttagagttac aagaaacttt ctggagtcct
    11521 gcttacgcaa acttagtaga ccaatttggt gtgatgtggc aaatttcaac agagttaaac
    11581 taatcgtttt taccatctga gcattgcaat gatagattta taaaaatact gattgcaaac
    11641 agagcagaaa aaatcaacgg atttcatgta ttgaatagct accttttggt agctatttta
    11701 ttcttaattg aagtaagcta ttttagctaa agtctattgt aatagggatt taaaggggta
    11761 ttagtgataa gttgaaggca ttatgatata ataggttgaa ttatattcag agatagagga
    11821 attaatatgt caaattatgc cattatttta gctgcgggta agggaactcg catgaaatct
    11881 gatttaccaa aagttatgca taaagtatct ggtattacca tgcttgagca tgttttccgt
    11941 agtgtgcaag ctattgagcc aagtaaaatt gttactgtta ttggacataa ggcggagctt
    12001 gtacgtgatg ttttaggtga caaatcagag tttgtaatgc aaacagaaca gctagggact
    12061 ggtcatgctg taatgatggc ggaagaagaa ttagcgacat caaaaggaca tacactagtt
    12121 attgcaggtg atacacccct gattactgga gaaagtttaa aaaacttaat tgattttcat
    12181 gtcaatcata aaaacgttgc aacaatctta acagccgatg ctgcaaatcc atttggatat
    12241 ggtcgtatta tacgtaattc agatgatgaa gttactaaaa ttgtagaaca aaaggatgct
    12301 aatgactttg agcagcaggt taaagaaatt aatactggaa cttatgtttt tgataatcaa
    12361 agtctatttg aagcacttaa agacattaat acaaataacg ctcaaggaga gtattatcta
    12421 actgatgtta ttggaatttt caaagaagcg ggtaaaaagg taggtgccta taaacttcgt
    12481 gactttgacg aaagtctagg agttaatgat cgtgtagcac ttgcaactgc tgaaaaagtg
    12541 atgcgtcagc gcattgcacg tcaacatatg gttaatgggg tcacagtggt taatcctgat
    12601 tccgcttata ttgatattga tgttgagatt ggagaagaat ctgtaattga gcccaatgtg
    12661 actttaaaag gacagacgaa gattggtaaa ggtactttat taaccaatgg tagttattta
    12721 gtagatgctc aagtcggaaa tgatgttacc atcactaatt cgatggttga agaatccatt
    12781 atttctgatg gagtaaccgt aggcccttat gcgcatatta gaccaggaac aagtcttgca
    12841 aaaggtgttc atattggtaa tttcgttgaa gtaaagggtt ctcaaatagg agagaatacg
    12901 aaggcaggtc atttaactta tatcggcaat gcagaggtag gttgtgatgt taactttggc
    12961 gcagggacga ttactgtcaa ttatgatgga caaaataaat ttaaaacaga aatcggaagt
    13021 aatgtcttta tcggaagtaa ttctactctg attgcaccac ttgaaattgg tgataatgct
    13081 ttaacagcag ctggatctac catcacagat aatgttccaa ttgatagcat agctattgga
    13141 agaggacgtc aagtcaataa agaaggatat gccaataaaa aaccacatca tccttcacaa
    13201 aagtaacact tatttgggag gttcttatgg attttgaaga aaaaacaatc aatcgtcaaa
    13261 ctgtttttga tggacaaatt atcaaagtag ctgttgatga tgtagagttg ccaaatggat
    13321 tgggacaatc caaacgtgaa cttgtttttc atggaggagc tgttgctact ttggcggtaa
    13381 ctcctgagca taaaattgtt ttagtgaaac agtatcgtaa ggctattgaa ggtatttctt
    13441 acgaaattcc agcagggaaa cttgagacgg gtgagtcagg ttctaaggaa gaagctgccc
    13501 ttcgggaatt agaagaagag actggctaca cagggaatct tgaaattctt tatagtttct
    13561 acactgctat tggtttttgt aatgagaaaa tcgtcctcta tctagctact gatttacaaa
    13621 aagttgaaaa tcctaggcca caagatgatg atgaggtcct tgagcttcta gagttaagtt
    13681 atgaggattg tatgcaaatg gtagaaaaag gcatgattca agatgcgaaa acgatcattg
    13741 ctttacaata ttacggttta aaaatgggag gtcattctta atgggaaaac cattgttaac
    13801 agatgatatg attgaacgtt caaatcgagg tgaaaaagtt tcaggtcaaa caatcttaga
    13861 tcaagaaact aaaattattt ctacagaaga tggcatggag caactaactg acgagaacgg
    13921 taaacatatc tacaagagtc gccggattga gaatgcaaaa cgaaatgaat ttcaacgtaa
    13981 gttgaattta gttttattca ttttgcttat tttgttggct ctcttgtttt atgcaatttt
    14041 taaattataa ggaaatatca tgaaaattgg aattattgca gccatggagg aagagttaaa
    14101 actccttgtg gaaaatcttg aggataaatc tcaagaaaca gtattatcaa atgtatatta
    14161 cagtggacgt tatggtgaac atgagcttgt tttagtacag tctggtgttg ggaaagtcat
    14221 gtcagcgatg agtgttgcta ttttagtaga aagctttaaa gttgatgcta ttattaatac
    14281 aggatcagct ggtgcagtag caacaggatt gaatgtagga gacgtcgttg tcgcagatac
    14341 attagtttat cacgatgtag atttaaccgc ctttggttat gactatggac aaatgtcaat
    14401 gcagccactt tattttcatt cggataagac ttttgtttct acttttgaag cagttttatc
    14461 taaagaagaa atgactagta aaattggttt aattgcaact ggggatagtt ttattgctgg
    14521 ccaggaaaag attgatgtta ttaaggggca ttttccacag gttttagctg tcgagatgga
    14581 aggtgcagca attgcccaag ctgctcaagc tacaggaaag ccctttgtgg tggtacgtgc
    14641 tatgagtgat actgcagctc atgatgccaa tatcacattt gatgaattta tcattgaagc
    14701 tggtaaacgc tcagcccaag tattaatggc atttttgaaa gcattataaa aaagtccgtg
    14761 tattcattag atacacgggc ttttttattt atgtagcaga tacatatatt tgacttgcga
    14821 tacgctcagg gattgttaga ttttcatcgt tatagatgat gtgataggtt tttgcataag
    14881 ggtcaatttg tgtcaattca atggtatcgt tgatatgcaa acggtgttgt tcgaggtatt
    14941 tcagtagctg aaaatcatca tgaacacgac tgataacata cgtgccaagc tgactaattt
    15001 ggtccaatgt catttggtta atttctacta aaaactctcc ctttttagga atagtcccac
    15061 cgtgtggaca aaattgtgga aagtctaaca acttctctaa ttgatcaaca aaagttgttg
    15121 atacagtatg ttctaaaact tccgcttctt tatgaatatc atcagcagta tagtgcaaat
    15181 ggttaactaa aaatacctcc actagacgat gtttacggta aaggtcagaa acaactaata
    15241 aaccttgttt agtaagataa tatcctaaat ctttatcttt aacaatcagc tgttcactta
    15301 tcattttttt aatcatttct gacacggcag gagccgagac ttgcatcatt tcagcgattt
    15361 tcttattgga aattttgttg tttttttcac ctaattcatg aatgcatttt aggtaatctt
    15421 ctttatttgg agtcatattc agtctttctt atacttctta aaatctattt ttctaagacc
    15481 attatatcaa aaaatactga aaaagtattt gacaaatact ttataatagg ttatgctaat
    15541 atcaaaatta agttaactta atttaataca taacttatat taattttaaa aggagcattt
    15601 tgatgaaaaa gtggttagtt attgtatcat gttttgtagc atttttaggc ttaggtgcct
    15661 gtgctaacaa gcaggatcaa gccaaaaacg aaaaattaaa agtagttgta accaactcta
    15721 ttttggcaga tataacaaaa aatatagccg gtaacaaaat cgacttacat agtattgtac
    15781 ctgttggtaa agatcctcat gaatatgagc cattaccagc agacgttgaa aaaacaactc
    15841 aagcagacct tgttttttac aacggaatta atcttgaaac aggcggtaat gcttggttta
    15901 caaaattaat aaagaacgct aagaagaaag aaaacaaaga ttactttgcg gttagcgatg
    15961 gagttgatgt tatttatcta aatggtcaaa gcggaaaagg taaagaagac ccacatgctt
    16021 ggttgaacct tgaaaatggt attatttact caaaaaatat tgctaagcaa ttaattgcta
    16081 aagatccaaa aaacaaagct acttatgaga aaaacagaga tgcttatgta gctaaattag
    16141 aaaaattgga taaagaagca aaatctaaat ttaatgccat tccagctaat aaaaaattaa
    16201 ttgttactag tgaaggttgc tttaaatatt tctcaaaagc ttatggagtt ccatcagcat
    16261 acatctggga aattaacact gaagaagaag gtacaccaga tcaaattact agcttagtta
    16321 agaaattaaa acaagtaaga ccttcagctt tattcgttga atcaagtgtt gacaagcgtc
    16381 ctatgaaatc tgtttcaaga gaatcaggta tcccaattta cgcagaaatc ttcacagact
    16441 caattgctaa aaaaggtcaa aagggtgaca gctactacgc tatgatgaaa tggaatcttg
    16501 ataagattgc agaaggttta gcaaaataag agtgagaatc gacattgaaa tcataatgaa
    16561 atcaatgatg tagtattctc gcttaaggct gagttgtaac aatgtcgaaa tcctaatcta
    16621 aaaactgact tagctttaat aaatacagag aagtggagtc tggttttata accagctcca
    16681 ttctactttt ggaggaaaaa tgattattag taaacactta agcgtaagtt atgataataa
    16741 tttagtgcta gaagatatta atttacgctt agaaggctcc ggaatcattg gtattttagg
    16801 gcctaatggg gcggggaaat ctacgcttat gaaagctctt cttggtttgg ttgattctac
    16861 tggagaatca gggattgggg gagatttgct acctttaatg gggagagtag catatgttga
    16921 acaaaaaact aatattgatt accaattccc tataactgtt ggagaatgcg tttctttagg
    16981 attatataag gaacgaggcc tcttcaagcg actatcaaaa actgactggg aaaaagtatc
    17041 ccgagtgatt gatcaagttg gtttgagagg gtttgaaaat agaccaatta atgccttatc
    17101 aggtggacaa tttcaacgta tgcttatggc aagatgtctc gttcaagaag cggattacat
    17161 ttttttagat gagccgtttg tagggattga ttccattagt gaacaaatca tagttaattt
    17221 actaaaaaaa ttaagtaagg ccggaaaatt aattttagtc gtccatcacg atttgagtaa
    17281 agttgatcat tattttgatc aagtaattat tttgaaccgt catctaattg cttgtggacc
    17341 aatagatcag gcatttaccc gtgagaattt aagtgctgct tacggtgatg ctattttgtt
    17401 aggacaagga gactagaatg tttacgaaat tttttgaagg cttattgact tatcattttt
    17461 tgcaaaatgc ttttataact gcaattgtga ttggtattgt agcgggtgca gtaggctgtt
    17521 ttattatctt gcgctcaatg tctttgatgg gtgatgctat ttctcacgct gttctaccag
    17581 gtgttgctat atcttttatt ttagggatta acttttttat aggtgctatt gtctttggtt
    17641 tgttgtcttc gattatcatt acttatatta aagaaaatag tgtcataaaa ggagatactg
    17701 ctattggaat tactttttcg tcctttttag ccttaggtat cattttaatt ggacttgcta
    17761 atagtacaac tgacctcttt catattcttt ttgggaatat tttggcagta caagatagtg
    17821 ataaatatat gactattatt gtgggattaa ttgtactgac tctgattact atcttcttta
    17881 aagaattatt attgacttct tttgatcctg ttttagcaaa atcaatggga atgagagttt
    17941 ctttttatca ttatttattg atgattttgt taacattagt tgcagtaaca gctatgcaga
    18001 gtgtgggaac aatattgatc gtagcacttt taattacacc agctgcaaca gcatatcttt
    18061 atgttaaaag tctcagaaca atgttgtttt tatcatctgc attaggagca gtagcatctg
    18121 ttctggggtt atatatcggt tatacattta atattgccgc aggatcaagt attgttttaa
    18181 catctacttt tatgttttta cttgctttcc tattttctcc taaacaaagt ttgtttaaaa
    18241 agaaacaata gtgttgttaa aaaatgagac tagaaaatgt tctagtctca tttttgatag
    18301 aattatttaa atttgtagtc tttaataact ttgatacttg taatagtaat tttttctttt
    18361 ggttgatctg attttgtaac ttctaccgaa gcaattttat ccacagtttc catgccagag
    18421 attacttgtc caaagacggt ataaccacca tctaaacttg gatttcctcc ctctgaatag
    18481 gctttaataa tcaccttagg aactttttta tctgacagtt gtttggaatg atcttgctgg
    18541 ctttgattga taaagaattg gctgccatta gtatcagctc cagcatttgc cattgctaaa
    18601 cttcccctga tattgtagag gtaaggggat atttcattaa caaaaccatt tccagaatct
    18661 ttctttttat ctttgctatt ccatatagat tttccaccgg tcccgtctcc attagggtca
    18721 ccagattgta tcataaaatc tttgataact ctgtggaaac ttaacccatt atagtatcct
    18781 tcttttgcat gtgttagaaa attttcaaca gctaaaggag catattttgg aaataacttg
    18841 atatttatat cacctttaga ggtttttata agcacttgtg cctcgttttt ggcaacagcc
    18901 ttgtccaatt gaggaaaagc ttttttatca gcttttaagg cttgcttagt ttttttgttt
    18961 agttgttcag tagcttcctt tgaagagttt tcagctagtt tttggtcaac ataacggtct
    19021 ccttttagag aacgctcaat tgactcgcaa ccacttaaag ttaatatact gacacaggct
    19081 aaaccaagat aaataatttt tttcatacat actccttgat taagttatta agatgattat
    19141 attttatcat aaatttttaa ataatttata aaagtaaata gtaaacctat tgggggactt
    19201 gttgaaatta tgatagaata gtatagcatg agttgataga gaaagtgagg aacaagtatt
    19261 tcacttgaaa tatggtattt atggcaaata agaaaaaaac aaaaggaaag aaaaccagaa
    19321 gacctactaa ggcagaaata gagcgtcaaa gagctattca aaggatgatt actgctcttg
    19381 ttttaacaat tattctcttc tttggtatta tcagattagg tatttttggt attacagtct
    19441 ataacgtcat ccgttttatg gtaggtagct tggcttactt atttattgcg gcaactttaa
    19501 tctaccttta tttctttaaa tggttgcgaa agaaagatag cttagtagca ggttttttga
    19561 tagcttcttt aggattattg attgagtggc atgcttacct tttctcaatg cctattttga
    19621 aagataaaga aattttgcgt tcaactgctc gattaattgt gtctgattta atgcaattta
    19681 aaatcactgt ttttgccggt ggaggtatgt tgggtgcttt gatttacaag ccaattgctt
    19741 ttctcttttc taatattggt gcctatatga ttggtgttct cttcatcatt ttgggtctct
    19801 ttttaatgag ttctctggaa gtttatgaca tcgtcgaatt tattagagct tttaaaaata
    19861 aagtggcaga gaagcacgag caaaataaaa aggagcgttt tgctaagcga gagatgaaaa
    19921 aagcaatcgc tgaacaagag cgcatagagc gtcaaaaagc tgaagaagaa gcttatttag
    19981 cttcggttaa tgtagaccct gaaacgggtg agattctaga ggatcaagct gaggacaatt
    20041 tggatgatgc gctaccacct gaggtaagtg aaacatcaac tccggtattt gagccagaga
    20101 tccttgctta tgagacatcg cctcaaaatg atcctttacc agtagagccg acaatttatt
    20161 tagaagacta tgattcgccg attcctaata tgagagaaaa tgatgaggaa atggtttatg
    20221 atttagatga tgatgtagat gatagtgata tagaaaatgt cgactttaca cctaaaacga
    20281 cactggttta taaattacca acgatagatt tatttgcacc agataagcct aaaaatcaat
    20341 ccaaagaaaa ggatttagtc cgaaagaata tcagagtttt agaagaaaca tttagaagtt
    20401 ttggtatcga tgtaaaagta gaacgtgctg aaattggacc atcagttact aaatatgaaa
    20461 ttaaaccagc agttggagtt cgtgtgaatc gtatttcaaa tctatctgac gacctagctc
    20521 ttgctcttgc agcaaaagat gtgcgtatag aagcaccaat tcctggaaaa tcattaatag
    20581 gtattgaagt tcctaactca gaaattgcaa cggtttcttt ccgcgaactt tgggaacaat
    20641 ctgatgccaa tcctgaaaac cttttagaag taccactagg aaaagctgtt aacggcaatg
    20701 ctcgcagttt taacttagct agaatgccgc atcttttggt agctggttca actggttcag
    20761 gtaaatctgt ggcagttaat ggaattattt caagtatttt gatgaaggca cgtccagatc
    20821 aagttaagtt tatgatgatt gatcccaaaa tggttgaatt atctgtttat aatgatattc
    20881 cacatttatt aatccctgtt gtaaccaatc cgcgtaaagc aagtaaggca ctccaaaaag
    20941 ttgttgatga aatggaaaat cgatacgagt tatttagcaa aattggtgtg cgtaatatag
    21001 caggttataa tacaaaggtt gaagagttta atgcttcctc tgagcaaaaa caaatacctt
    21061 tgcctttaat cgttgtcatt gtagatgaat tggctgactt gatgatggtt gctagtaaag
    21121 aagttgaaga tgctattatt cgtttggggc aaaaagcacg tgctgcaggt atccatatga
    21181 ttcttgcaac tcaacgtcca tccgtagatg ttatttctgg tttgattaaa gcaaatgttc
    21241 cgtcgcgtat tgcatttgct gtttcaagtg gtactgatag ccgtacgatc cttgatgaaa
    21301 atggtgctga aaagctcttg ggacggggtg acatgctctt taagcctatt gatgagaatc
    21361 atccagtacg actacaaggt tcctttattt cagatgatga tgttgaaagg atcgttggtt
    21421 ttatcaaaga ccaagccgag gctgactatg atgatgcctt tgatcctgga gaagtatctg
    21481 aaacagataa cggctctggt ggtggcggcg gagtacctga aagtgatcct ctttttgaag
    21541 aagccaaggg actcgtttta gagacgcaaa aagcaagtgc ctcaatgatt caacgccgat
    21601 tgtctgttgg tttcaataga gcaacaagac taatggaaga attagaagca gcgggggtta
    21661 ttggtccagc agaaggaacc aagccacgaa aagttttaat gactccaact ccgagtgaat
    21721 aagaaaaaac taatctgaca agaaattgtc ggattagttt tttatgttat aatgaagcaa
    21781 atagatgaag aagaaggtta cgaatgcata ttgagactgt tattgatttc aaagaattag
    21841 gaaaaagata tcgttttaaa aatcctacaa aagaattaat agctgatact ttagaacaag
    21901 tcttagaagt gataaaagaa gttgattatt atcaatctca aaattattat gttgttggtt
    21961 atttatctta tgaagcatct gctgcttttg attcacattt taaagtttct caacagaagt
    22021 tggctggaga acatctagct tattttacag tacataaaga ttgtgagaac gaagcttttc
    22081 ctttaagtta tgaaaatgtt agattagcag ataattggac tgctaatgtt tctgagcaag
    22141 aatatcaaga ggcaattgct aatattaaag gacaaattag acaaggaaat acttatcaag
    22201 taaattatac actagagctt agccaacaat tatgctcgga tccctttagt gtttatgaac
    22261 gcctaatggt agaacaagga gcgggataca atgcttatat tgcctacgac gataaaagga
    22321 ttttatctgt aagcccggaa ttgttcttta agaagaagga tgaagtctta acaactagac
    22381 caatgaaggg aacaagcgct agaaagccta cttatcaaga agatgtggcg gagcgagatt
    22441 ggttggcaaa tgatcctaaa aatcgttctg aaaatatgat gattgttgat ttattaagaa
    22501 acgatatggg ccgtatctgt gatgttggaa cagttaaggt aaaaaaacta tgtcaagtgg
    22561 agcagtatgc aactgtgtgg caaatgacat caactattga aggagtttta tcgccagaag
    22621 tgacacttat gtctattttt caagctctat atccttgtgg atctatcact ggagctccta
    22681 agatatcaac aatggctatt attaatgagt tggaaaaacg gccaagaggt atttattgtg
    22741 ggacgatagg actttgcatg ccagacggac aagctatttt taacgttcct attcgcacag
    22801 tacaaatgaa aggtcaacaa gcctattacg gcgttggtgg aggtattacg tgggagagcc
    22861 agacagattc tgaatatgaa gaaacccgtc aaaaatcagc tgttttaaca cgtgtcaatc
    22921 caaaatttca attaataact acaggaagag tgactgaaaa taaacttctt ttctctcaac
    22981 aacatgtcga gagattagtt gaatcagcgt cttattttgc ttattctttt gataaaagta
    23041 aattcgaaag ggaactgaaa aaataccttc atcagctaga tgagaaggat taccgcttga
    23101 aaataatgct tgataaaact ggtaaggtaa cgtttgaggt aaaacaacta gtgaatttat
    23161 caaaaaagtt tttaacggca gaggtggtcg tacaagacta ccctattaaa ttatccccgt
    23221 ttacttattt taaaacttct tatcgcccac atattattga aggtcagaat gaaaagatat
    23281 ttgtatctcc tgaggggttg ctattggaaa caagtattgg gaatattgtt ttagaaaaaa
    23341 atggaaggtt tttaacccca gatttatcag aaggagggtt gaatgggatt tatcgtcgtc
    23401 atctccttga aaaccaaaaa gtaattgaag caccactaac tttaaaagat ttagaatcag
    23461 ccgatgctgt atacgcctgt aatgctgtta gagggcttta tcctctaaac ctaaagtaaa
    23521 aagagctgga aatttatcca actcttttta ttgtactttg ataaagatga taaccaataa
    23581 tcctaggtaa aaaaggaaag ttagaataaa gccagatctc caaaataatt ttaaaaaacg
    23641 tttataagaa aaaagagtct ttgttcgtag aagctttatt gtaattccga ttgccagtaa
    23701 tgaaatgaga atcagatagt aaggtaaaag a
//
LOCUS       DATRNK010000026        23638 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.026, whole genome shotgun sequence.
ACCESSION   DATRNK010000026 DATRNK010000000
VERSION     DATRNK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23638
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.026"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            150..1028
                     /locus_tag="VBC52_001048"
     CDS             150..1028
                     /locus_tag="VBC52_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6882644.1"
                     /translation="MIESIFEETAKGKVSRHVLLTILLALILLFTADFLTYIIIELII
                     SLDFWKLEWDSDIVQLIILLATSISTVMLFMWVKFKEKRPISSLGLFKEGALTELSRG
                     WLVGSVLFGACLILTIISGSGVIETVRFTADKLIWFLIFAVGWQVQSASVEILCRGWL
                     LPVSATKYSKIVSVSISSIFFGLLHSANNHVSLISIFNLCLFGLFLSLYVILKGNIWG
                     ACGIHGAWNCVQGSVFGIEVSGEPMLSNSLVHVKTYGADWISGGKFGVEGSMITSIVL
                     IVACYWLYQKSNFPYD"
     gene            1135..1725
                     /locus_tag="VBC52_001049"
     CDS             1135..1725
                     /locus_tag="VBC52_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEO6882645.1"
                     /translation="MPQNPTSNPEKKTYLLSELVDCFKGKAVPSKAEAGDIRIINLSD
                     MSPLGIDYHNLRTFQDEQRSLLKYLLQEGDVLIASKGTVKKVAIFEEQDYPVVASANI
                     TILRPTQHIRGYYLKLFFDSEEGQQALENANKGKAVMNISTKELLNIAIPSIPLFRQD
                     YLIQRYKQGLNDYKRKIARAEQEWERIQNDIRQQLS"
     gene            1759..3030
                     /locus_tag="VBC52_001050"
     CDS             1759..3030
                     /locus_tag="VBC52_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEO6882646.1"
                     /translation="MNEIKCPHCGTAFAINESEYHQLLEQIRGDAFDKEVSERLEKER
                     LILGEQAKNQLQEVVVEKDKEIAKLQYKVKQFLIEKDNLLKDNEYQLAEQLNQKDMML
                     RDLENQIDRLRLEHENSLQEALTKVERERDAIQNQLHIQEKEKDLALASVKSDYEVQL
                     KAANEQVEFYKNFKAQQSTKAVGESLEHYAETEFNKVRHLAFPNAYFEKDNTLSSRGS
                     KGDFIYREKDENDLEFLSIMFEMKNESDDTIKKHKNEDFFKELDKDRREKSCEYAVLV
                     TMLEADNDYYNTGIVDVSHKYPKMYVIRPQFFIQLIGILRNAALNTLKYKQELALMKE
                     QNIDITHFEEDLDIFKNAFAKNYNSASKNFQKAIDEIDKSIKRMEAVKAALTTSENQL
                     RLANNKLDDVSVKKLTRKNPTMKAKFDALKD"
     gene            3043..3474
                     /locus_tag="VBC52_001051"
     CDS             3043..3474
                     /locus_tag="VBC52_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6882647.1"
                     /translation="MKIRTATLDDSEKLVPLYQELGYAISLSEIQSILKVILTHSDYG
                     FLIAEDNGKLLAFVGYHKLYFFEKSGTYYRILALVVNEKHRRKGIASQLINHVKQLAK
                     TDGSEVLALNSSLKEYRQEAYHFYENLGFKKVSTGFSYYLD"
     gene            3566..4450
                     /gene="truB"
                     /locus_tag="VBC52_001052"
     CDS             3566..4450
                     /gene="truB"
                     /locus_tag="VBC52_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEO6882648.1"
                     /translation="MITGIINLKKEAGMTSHDAVFKLRKILHTKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTESGKIYEGEITLGYATSTEDSSGEVISRTPLTQSDLSEDVV
                     DHAMKSFTGPITQVPPMYSAVKVNGKKLYEYARSGEEVERPKRQITISEFRRTSPLYF
                     EKGICRFSFYVSCSKGTYVRTLAVDLGIKLGYASHMSFLKRTSSAGLSITQSLTLEEI
                     NEKYKQEDFSFLLPIEYGVLDLPKVNLTEEDKVEISYGRRILLENEADTLAAFYENRV
                     IAILEKRGNEFKPHKVLL"
     gene            4463..5395
                     /locus_tag="VBC52_001053"
     CDS             4463..5395
                     /locus_tag="VBC52_001053"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEO6882649.1"
                     /translation="MKIHYINDYKDIQAKEDCVLVLGYFDGLHLGHKALFDKAKKIAT
                     EKNLKIVVLTFNETPRLTFARFQPELLLHLTSPEKRSEKFQEYGVDELYLMNFTSHFS
                     KVSSDLFIKKYIYGLRAKAAVVGFDYKFGHNRTSGDYLARNFKGPVYIIDEISEGGEK
                     ISSTRIRQLITEGNVEKANQLLGYEFSTCGMVVHGDARGRTIGFPTANLAPINRTYLP
                     ADGVYISNVLINGKYYRAMTSIGKNITFGGTELRLEANIFDFDGDIYGETIEIFWLKR
                     IREMVKFNGIDDLVKQLKKDKEIALNWKKDSQTL"
     gene            5438..5851
                     /locus_tag="VBC52_001054"
     CDS             5438..5851
                     /locus_tag="VBC52_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEO6882650.1"
                     /translation="MITLFLSPSCTSCRKARAWLSKHEVAFEEHNIITSPLNKEELLQ
                     ILSFTENGTEDIISTRSKVFQKLAIDVDELSTSSLMELISENPSLLRRPIILDKKRMQ
                     IGFNEDEIRAFLPRDYRKQELKQATIRAEIEGKHD"
     gene            5844..6125
                     /locus_tag="VBC52_001055"
     CDS             5844..6125
                     /locus_tag="VBC52_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEO6882651.1"
                     /translation="MISSNYSYPLDPSWNTEDITKVLRFLNQVEHAYENSIKVDDLLD
                     SYKEFKKVVKSKAQEKQIDREFQRTSGYSTYQAVKAAQQQAKGFISLGR"
     gene            6115..6879
                     /locus_tag="VBC52_001056"
     CDS             6115..6879
                     /locus_tag="VBC52_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEO6882652.1"
                     /translation="MDAKFDFAKQLVYKAGQFIKSEMQNTFDVEEKSRFDDLVTSLDK
                     KTQKLLIQEIIQHYPDDNILAEEDDVRSPIAQGNVWVLDPIDGTVNFIVQKDNFAVML
                     AYYEEGVGQFGIIYDVMADILYSGGGHFDVYANDKKIVPFQECPLERCLLGVNSAMYA
                     ENDCGIAHLASETLGVRIYGGAGISMAKVMQGKLLAYFSYIQPWDYAAAKIMGETLGF
                     TLLTLDGEEPNYSTRQKVMFLPKSKLNLIQSYLTKK"
     gene            6947..8257
                     /locus_tag="VBC52_001057"
     CDS             6947..8257
                     /locus_tag="VBC52_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEO6882653.1"
                     /translation="MKLPNEFIEKYQTILKDEAEAFFDSFEQKPISAYRTNPLKEKQL
                     DFPNAIPSTPWGHYGKISGKSIEHTTGLVYSQEPAAQIVAQIAEPQEGMKVLDLAAAP
                     GGKTTHLLSYLNNTGLLVSNEISNKRSKILVENVERFGARNVIVTNESSQRLAKCFNS
                     FFDLIVFDGPCSGEGMFRKDPQAIQYWHKDYPTECAQLQRDILKEAIKMLAHGGILVY
                     STCTWSPEENEEVVNWLLQEYDYLELVDIPKLNGMVEGINVPQVARMYPHHFQGEGQF
                     VAKLRDTRSKEAQKIKPKAQKINKMQLQLWQQFAQDHLKIDLNGVLDVFGDQLYLLPN
                     GLPDLSKLKIARNGLHLGTFKKNRFEPSFALGMALSEHDLVQSIEIDIEQFEVYVSGN
                     VVKLDKTVPNGWYQILVKGNGLGFAKVTNNTLKNYYPKGLRFQT"
     gene            8436..9302
                     /locus_tag="VBC52_001058"
     CDS             8436..9302
                     /locus_tag="VBC52_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEO6882654.1"
                     /translation="MKMKKNIFLLTLLATVLLTLSGCANWIDKGQSITSVGSTALQPL
                     VEAAADEFGKTNLGKTINVQGGGSGTGLSQVQSGAVQIGNSDLFAEEKDGIDAKKLKD
                     HQVAVAGLAVIVNKKVNVKNLTTHQLRDIFAGKIKNWKEVGGQDLDISIINRAASSGS
                     RATFDNTIMGNVAPIQSQEQDSNGMVKSIVSQTPGAISYLAFAYVDKSVGTLKLNGFA
                     PTAKNVTTDNWKLWSYEHMYTKGNETGLTKEFLDYMKSDKVQSSIVQHMGYISINDMK
                     VVKDAEGKVTPK"
     gene            9348..10265
                     /gene="pstC"
                     /locus_tag="VBC52_001059"
     CDS             9348..10265
                     /gene="pstC"
                     /locus_tag="VBC52_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO6882655.1"
                     /translation="MKNQELAKKLTSPSKNSRLEKFGKGITFLSLALIVFIVAMILIF
                     VAQKGLSTFFVDGVKLTDFLFNTKWEPSAKSFGAFPMIAGSFIVTILSAIIATPFAIG
                     AAVFMTEISPKYGSKILQPAVELLVGIPSVVYGFIGLQIIVPFVRSIFGGTGFGILSG
                     VCVLFVMILPTVTFMTVDSLRAVPRHYKEASLAMGATRWQTIWRVILNAARPGIFTAI
                     VFGMARAFGEALAIQMVVGNSAILPTSLTTPAATLTSVLTMGIGNTVMGTVQNNVLWS
                     LALVLLIMSLAFNTVIKLITREGKKNYER"
     gene            10255..11142
                     /gene="pstA"
                     /locus_tag="VBC52_001060"
     CDS             10255..11142
                     /gene="pstA"
                     /locus_tag="VBC52_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO6882656.1"
                     /translation="MNAKKADKLATTILYSIAAIIVTILASLLIFILVRGLPHVSWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSLGAGIYLSEYAKKGRLTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFQYGFSIISGALALTVFNLPQMTRSVEDSLQNVH
                     HTQREAGLALGISRWETVIYVVVPEALPSIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNVLSVTSPISIFRQAETLAVHIWKVNSEGTIPDATQVSAGSAAVLLVVILIF
                     NLSARSIGKKLHSKLTSSN"
     gene            11154..11957
                     /gene="pstB"
                     /locus_tag="VBC52_001061"
     CDS             11154..11957
                     /gene="pstB"
                     /locus_tag="VBC52_001061"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6882657.1"
                     /translation="MAEYNWDERHIITFPEENSALTTKDLHVYYGEKEAIKGIDMQFE
                     KNKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGQIMYEGIDVNAQDINVYEMRKH
                     IGMVFQRPNPFAKSIYKNITFAYERAGVKDKKFLDEVVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAIAVKPEILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGDLIEYDKTNNIFQNAKCQSTSDYVSGRFG"
     gene            11969..12727
                     /gene="pstB"
                     /locus_tag="VBC52_001062"
     CDS             11969..12727
                     /gene="pstB"
                     /locus_tag="VBC52_001062"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6882658.1"
                     /translation="MTQPILQVSDLSVYYNKKKALKEVSMDFYPNEITALIGPSGSGK
                     STLLRAINRMGDLNPEVTLTGAVMYNGHNVYSPRTDTVELRKEIGMVFQQPNPFPMSV
                     FENVVYGLRLKGIKDKATLDEAVETSLKGASIWDEVKDRLHDSALGLSGGQQQRVCIA
                     RTLATKPKIILLDEPTSALDPISAGKIEETLHGLKDQYTMLLVTRSMQQASRISDRTG
                     FFLDGNLIEYGNTKEMFMNPKHKETEDYITGKFG"
     gene            12761..13414
                     /gene="phoU"
                     /locus_tag="VBC52_001063"
     CDS             12761..13414
                     /gene="phoU"
                     /locus_tag="VBC52_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO6882659.1"
                     /translation="MLRSKFDEELDKLHNQFYAMGIEAIGQIKKTVRAFVSHDRELAK
                     EVIEDDVTLNNFETKLEKKSLEIIALQQPVSQDLRTVITVLKATSDVERMGDHAAAVA
                     KATIRMKGEERIPAVELEINNMGKAVKNMLEEALTAYINGDDEKAYEVAAMDEIVDDY
                     FRDIQKMVVETIQKHPDVAFAAKEYFQVLMHLERIGDYGKNICEWIVYLKTGKIIEL"
     gene            13560..16109
                     /locus_tag="VBC52_001064"
     CDS             13560..16109
                     /locus_tag="VBC52_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEO6882660.1"
                     /translation="MKTVEHFVTKFVPENYNLFLDINRQTKTFSGNVAVSGEALDNNI
                     SFHQKGLTIKSVLLDNQPLDFQLDEDNEAMHIQLHETGSMVLVFEFSGHITDNMTGMY
                     PSYYTVNGIKKEVISTQFESHFAREVFPSIDEPEAKATFDLSLKFDQKEGEIALSNMP
                     EINAEQRQETGLWTFDTTPKMSSYLLAFALGELHGKTTHTKNGTLVGSYATKAHQLNE
                     LDFSLDIVVRVIEFYEDYFGVRYPIPQSLHVALPDFSAGAMENWGLVTYREVYLLVDE
                     NSSVSSRQQVALVVAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIDYIEPKLN
                     IFEDFQTGGLPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRW
                     LGDTDFAAGLKIYFEKHQYQNTIGRDLWNALSQTSGKDVAAFMDSWLEQPGYPVMAAK
                     IEEDELILTQKQFFIGEHEDKSRLWQIPLNSNWEGIPEILTEETVVIPNFSQLAEKNK
                     ENGALRFNTENTAHYITNYQGQLLEHIISDLPLMDNISKLQIVQERHLLAESGMISYS
                     SLIPLVSLLSQETSYLVNSAIKSVIDGLSLFVQEDSQDEFDFKEFVNKLSAFNFNRLG
                     FEKREGEGDDSEMVRHLSLSLALYSDNEHAIEEAHHIFKAHENNIAAIPAAIRLLVLT
                     NEMKHFESKELSHLLLETYSTTTDGNFKRQLASALSHTTDSKTLKKLLSDWKNKDIVK
                     PQDLAMSWYATFLKNSFTQESVWEWAQENWEWIKATLGGDMSFDKFVIYPSSSFKTEE
                     RLEQYKNFFEPQLSDMAISRNISMGIKEISARVLLITKQKEEVINTIKKYI"
     gene            16271..16951
                     /locus_tag="VBC52_001065"
     CDS             16271..16951
                     /locus_tag="VBC52_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6882661.1"
                     /translation="MIKILLIEDDLSLSNSVFDFLDDFADVMQIFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESIDDKGQGFDLGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNDNSLIYGDIRVDMSTNSTFVNQTEVELLGKEFDLLVY
                     FLQNQNVILPKSQIFDRIWGFDSDTTISVVEVYVSKVRKKLKGTLFSENLQTLRSVGY
                     ILKHVETD"
     gene            16935..18248
                     /locus_tag="VBC52_001066"
     CDS             16935..18248
                     /locus_tag="VBC52_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6882662.1"
                     /translation="MSKPIKISKFKKNVSDSHFIHFFTVFSGIFLVMTVIILQVMRYG
                     VYSSVDSSLKYISTHPKNYINMVMSRTAAYLDNSNIASVKLKPGGQTVANTDIILFTS
                     EEEVINYFDAFSNYQFLKPNKKNLGGISELTLTNIFGQDETYHAVTVKVNNPAYPNVT
                     YMTAIVNIDQLVNAKERYEKIIIFVMTTFWIISIGASIYLAKWAQKPIIENYERQKAF
                     VENASHELRTPLAVLQNRLETLFRKPNATILENSENIASSLDEVRNMRILTTNLLNLA
                     RRDDGIKPELAVIKPTLFDSIFENYDLIAQENGKNFTGHNMIQDSFKTDKTLLKQLMT
                     ILFDNAIKYTDNDGSIDFTISETDKYLFLEIADNGPGISEEDKVRIFDRFYRVDKART
                     RQQGGFGLGLSLAQQIVNSLRGNITVIDNKPRGSIFKIKLPKVKG"
     gene            18337..18669
                     /locus_tag="VBC52_001067"
     CDS             18337..18669
                     /locus_tag="VBC52_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEO6882663.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     SGVSRQAVYDNIKRTEKILEAYEMKLHMYSDYIVRSQIFDDILEKYTDDAFLQEKISI
                     LSSIDNRD"
     gene            18687..20252
                     /gene="ffh"
                     /locus_tag="VBC52_001068"
     CDS             18687..20252
                     /gene="ffh"
                     /locus_tag="VBC52_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEO6882664.1"
                     /translation="MAFESLTERLQGVFKNIRGKKKLSEKDVQEVTKEIRLALLEADV
                     ALPVVKTFIKHVRERAVGHEIIDTLDPTQQIVKIVNEELTDLLGAETSEIEKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEDNARPMMIAADIYRPAAIDQLKTLGSQINVPV
                     FDMGTNHSAVEIVTKGLEQARENRNDYVLIDTAGRLQIDATLMQELHDVKAIAQPNEI
                     LLVVDSMIGQEAANVAEEFNRQLSISGVVLTKIDGDTRGGAALSVREITGKPIKFTGT
                     GEKITDIETFHPDRMASRILGMGDLLTLIERASQEYDEKRSMELAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMLPGMANNPAMKNFKVDENEIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVDVNKFIKDFNQAKQMMQGVMSGDMNKMMKKMGIDPNNLP
                     KDMPGMDGMDMSNLEGMMGQNGMPDLSSLGGDMDFSQMFGGGLKGKVGAFAAKQSMKR
                     MANKMKKAKKKRK"
     gene            20319..21005
                     /locus_tag="VBC52_001069"
     CDS             20319..21005
                     /locus_tag="VBC52_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEO6882665.1"
                     /translation="MYLALIGDIINSKQILERETFQQSFQQLMTELSDVYGEELISPF
                     TITAGDEFQALLKPSKKVFQIIDHIQLALKPVNVRFGLGTGNIITSINSNESIGADGP
                     AYWHARSAINHIHDKNDYGTVQVAICLDDEDQNLELTLNSLISAGDFIKSKWTTNHFQ
                     MLEHLILQDNYQEQFQHQKLAQLENIEPSALTKRLKASGLKIYLRTRTQAADLLVKSC
                     TQTKGGSYDF"
     gene            20995..21768
                     /locus_tag="VBC52_001070"
     CDS             20995..21768
                     /locus_tag="VBC52_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEO6882666.1"
                     /translation="MISDFLRDNPILTLLFCAHFLADFQWQSQSLADSKSHSWRGLWR
                     HLLIVFLPLAALMILIPETTLLNLSIWGSHIVIDSIKKLSYPWVEEGHFQKAAFIIDQ
                     LAHYTCIIVFYHALPTYLPPNHWLLPIKHFIVIALVFIIITKPINIVFKIFFNKFQAK
                     ELSSLLTQEKTKIMKEKSEDHEETIEGAGAMIGNLERLIMAILLISGQYAAIGLVFTA
                     KSIARYDKISKSQVFAEYYLIGSLFSIISVLITHWLLLV"
     gene            complement(21801..23462)
                     /locus_tag="VBC52_001071"
     CDS             complement(21801..23462)
                     /locus_tag="VBC52_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6882667.1"
                     /translation="MSSFNRKKLKFLGISLATLTATTVTLVACGNESKNSGDNKVINW
                     YIPTEISTLDISKNTDAYSNLAIGNSGSNLLRIDKEGKPKPDLAKKVSVSSDGLTYTA
                     TLRDNLKWSDGSKLSAEDFVYTWRRIVDPKTASEYAYLATESHLLNADKINSGDIKDL
                     NKLGVTAKGNQVTFKLTSPCPQFKYYLAFSNFMPQKQSYVEKVGKDYGTTSKNQIYSG
                     PYLVKDWNGSNGKFKLVKNKYYWDSKHVKTNSVIVQTIKKPDTAVQMYKQGQIDFAEI
                     SGTSAIYQANKNNKDVVDASDARTTYIIYNQTGSVKALTNQKIRQALNLATDRKGVVK
                     AAVDTGSTPAESLVPKKLAKLPNGEDLSKYTAPGYTYNTSKAQKLFKEGLAEVGQSSL
                     KLTITADSDSPAAKNAVDYVKSTWESALPGLTVEEKFVTFKQRLEDAKNENFDVVLFS
                     WGGDYPEGSTFYGLFTTNSAYNYGKFSSKEYDNAYQKAITTDALKPGDAANDYKTAEK
                     ALFDQSYYNPVYYLGKKGLQNSKLKGLVRNSTGLNVDFTYAYKTE"
BASE COUNT         8013 a         3966 c         4326 g         7333 t
ORIGIN      
        1 taaaaaaact caatatgtta gatgccttga aatcagtaga ttagtttata ataaagatta
       61 tcttttctaa tcttttcgat aacgattgac agcaagtaat cttaattgta taatataaca
      121 gtataaagtt agataaaagt gagatattta tgatagagtc tatttttgag gaaactgcaa
      181 aaggtaaagt atcaaggcat gtccttttaa ctatattatt ggctttgata ttactattta
      241 cagcagattt ccttacctat attattattg aattgattat ctcgcttgat ttttggaaac
      301 tagagtggga tagcgatatc gtccagttaa tcattttact agcaacttca attagtacgg
      361 taatgttatt tatgtgggtg aaatttaaag agaagcgtcc aattagttct ctagggctat
      421 ttaaagaagg agccctaaca gaattatcta gaggatggtt agtcgggagc gttctctttg
      481 gagcctgctt aatattaaca ataatttcag gctctggagt tatcgaaact gttcgtttta
      541 cagctgataa attgatttgg tttcttattt ttgctgttgg atggcaggta caaagcgcat
      601 ctgttgaaat attgtgtcgt ggatggttac ttccggtgag tgccacaaag tatagtaaga
      661 tagtttctgt cagtatcagt tcaatctttt tcggtcttct gcatagcgct aataatcatg
      721 taagccttat ctcgattttc aatctgtgtc tctttggact atttctatcg ctctatgtta
      781 ttttaaaagg taacatctgg ggggcgtgtg gtattcatgg cgcctggaac tgtgtacaag
      841 ggagtgtctt tggaatcgaa gttagtggag agccaatgtt gtctaactcc ctagtccatg
      901 ttaaaaccta tggtgctgac tggattagcg gtggaaaatt tggtgtggaa ggaagcatga
      961 ttaccagtat tgttcttatc gtagcatgtt attggcttta tcaaaaatcg aattttcctt
     1021 acgactaata gatttaatct aaataagctt ctcttactga aagcttattt tttattttag
     1081 tattaaaaaa ctatgttttg tgatataata ttttaaagac gaaaggagtc tattgtgcca
     1141 caaaatccaa caagtaaccc agagaaaaaa acttatttat tatctgaatt ggtagactgc
     1201 tttaaaggta aagctgtgcc ttcaaaagca gaagcaggtg atatccgtat tattaatttg
     1261 agtgatatgt ctcctttagg aattgactac cataacttaa gaacattcca agatgaacaa
     1321 cgatcgcttt taaaatatct acttcaggaa ggcgatgttt taatcgcttc aaaaggaact
     1381 gtcaagaaag ttgctatttt tgaagagcaa gattaccctg tcgttgcctc tgctaatatt
     1441 acaatactaa ggcctacaca acacattaga gggtactact taaagctttt ttttgattca
     1501 gaagaaggtc aacaggcact tgagaatgct aacaaaggaa aagctgtgat gaatattagc
     1561 acaaaggaac tgttaaatat tgcaatacca agtatcccgc tttttagaca ggattatctt
     1621 atccaacgtt ataaacaggg gctcaacgat tacaaacgta agatagcacg tgcagagcag
     1681 gagtgggaac gtatccaaaa tgacatcaga caacaactta gctagaacca aatacaaaac
     1741 atcataggaa gagttatcat gaacgaaatc aaatgccctc attgtggaac agcttttgcc
     1801 atcaacgagt ctgaatacca tcaattacta gaacaaattc gcggagatgc ttttgacaaa
     1861 gaagtaagtg aacggttgga aaaagaacgt ctaatattag gggagcaagc aaaaaatcaa
     1921 ttacaggaag ttgttgtaga aaaagacaag gagatagcta aacttcagta caaagtcaaa
     1981 caatttctta tagaaaaaga caatcttctc aaagacaatg agtaccaact cgctgagcaa
     2041 ttaaatcaaa aagacatgat gcttcgcgac cttgaaaacc aaatcgatag actacgttta
     2101 gagcatgaaa atagcttgca agaggcgcta acaaaagtcg aacgagaaag agatgcaata
     2161 caaaatcagt tgcacattca agaaaaagaa aaagatttag ctttagcttc agtaaaaagt
     2221 gattatgaag tacaactaaa ggcagccaat gaacaagtag aattctataa aaacttcaaa
     2281 gctcaacagt ctactaaagc agtaggagaa agtttagaac attatgctga aacagaattt
     2341 aataaagtgc gacatttggc ctttcctaat gcttattttg agaaggacaa tacattatca
     2401 agtcgtggct caaaggggga ctttatctat cgagaaaagg atgaaaatga ccttgagttt
     2461 ttaagtatca tgtttgaaat gaaaaatgag tctgatgata ctatcaagaa gcataaaaat
     2521 gaagattttt tcaaagaatt agataaagat cgtcgtgaaa aatcttgcga atacgcagtt
     2581 ttagtaacta tgcttgaagc agacaatgac tattataata ctggaattgt tgatgttagt
     2641 cacaaatacc ctaaaatgta cgttatacgt ccacaatttt ttatccaatt aattggtatt
     2701 ctaagaaatg cagcactcaa taccttaaaa tataaacaag agcttgcttt gatgaaagaa
     2761 caaaatattg acatcacaca ttttgaagaa gatttagata ttttcaaaaa tgcatttgct
     2821 aaaaattata attctgcaag caaaaatttc cagaaagcaa tcgatgaaat agataaatct
     2881 attaaacgta tggaagctgt taaggctgct ttaacaacgt ctgaaaatca actacgtctt
     2941 gcaaataata aattagacga tgtttctgtc aagaaattaa caagaaaaaa tccaacaatg
     3001 aaagcaaaat tcgatgctct aaaagactaa gaggttatga ttatgaagat tagaactgct
     3061 acactagacg atagtgaaaa attagtccct ttgtatcaag aattaggata cgcaatatca
     3121 ttatcagaga ttcaatctat tttaaaggtt attcttactc attcagatta tggctttttg
     3181 atagctgagg ataatggcaa actcctcgcc ttcgttggat atcataaact ttactttttt
     3241 gaaaaaagtg ggacttacta tcgtatttta gcactagtag ttaatgaaaa gcaccgtcgt
     3301 aaaggcatcg ctagtcaact tattaatcac gttaaacagc tagctaaaac agatggaagt
     3361 gaagtactgg ctttaaatag cagtttaaaa gaatatcgtc aggaagctta tcatttttat
     3421 gaaaacctag ggttcaagaa ggttagtaca ggcttttcat actatttaga ttaggtataa
     3481 atccttatag cactatacca aaagagcgta agtttttacg ctcctttctt tgtgttaccg
     3541 cttatttact gatataatat aaaacatgat tacaggaata attaatttaa agaaagaagc
     3601 tggaatgaca tcacacgatg ctgtattcaa gcttcgaaag atattacata caaaaaaaat
     3661 aggacacggc ggaactcttg atccagatgt tgttggggtt ttaccaattg cggttggaaa
     3721 agccactcgt gttattgaat atatgacaga aagtggcaaa atttatgaag gagaaatcac
     3781 tttaggatat gccacaagta cagaagattc tagtggagag gtcatttcaa gaactccact
     3841 aactcaatcc gatcttagcg aggatgttgt ggatcatgcg atgaaaagtt ttaccggtcc
     3901 aattacacaa gttcctccta tgtattcagc agttaaagta aatggtaaaa aactctatga
     3961 atatgctcgt tcaggagaag aagtagagcg acctaaacga caaataacaa tttctgaatt
     4021 tagacgcaca agcccattgt acttcgaaaa aggcatctgc cgttttagtt tctatgtctc
     4081 atgcagtaaa ggtacctatg ttaggacgtt agctgttgat ctaggaataa aactaggata
     4141 tgctagccat atgtctttcc taaaaagaac ctcatctgca ggattatcta ttacacaatc
     4201 attaacgctt gaagaaatta atgaaaaata taagcaagaa gatttttcat ttttacttcc
     4261 aattgaatat ggtgttttag atctcccaaa agttaaccta actgaggaag ataaagttga
     4321 aatctcatac ggacgtcgta tcttattaga aaatgaagca gataccttag cagcttttta
     4381 tgaaaataga gttatcgcta tactagaaaa acgtggcaat gaatttaaac cacacaaagt
     4441 attgttataa agaaagtcaa tcatgaaaat ccattacatt aatgattata aagatattca
     4501 agctaaagaa gattgcgtct tagttttggg ctattttgat ggcttacatc tgggacataa
     4561 agccctcttt gataaagcta aaaaaatcgc gaccgaaaaa aatttgaaaa ttgtcgtctt
     4621 aacgtttaat gagacacctc gtctaacctt cgctcgcttc caacctgaat tattgctaca
     4681 tcttacctct ccagaaaaac gttctgaaaa atttcaagag tatggcgtcg atgagttata
     4741 tttaatgaat tttaccagtc atttttcaaa agtatcttct gaccttttta ttaagaaata
     4801 tatttatggt ctcagagcaa aagctgctgt tgtcggtttt gattataagt tcggtcataa
     4861 tcgtaccagt ggtgattatc tagcgcgtaa tttcaaggga ccagtctata ttattgatga
     4921 aatttctgaa ggtggtgaaa aaatctcttc tacacgtatt cgtcaattga ttacagaggg
     4981 taatgttgag aaagctaacc aacttttggg ttatgagttt tcaacctgtg gcatggtagt
     5041 acatggagat gctagaggac gaactatagg gttcccaact gctaatctag ctcctattaa
     5101 tcgcacttac ttacctgcag acggtgttta tatttccaat gttctgatta atggaaaata
     5161 ttatagagca atgacgagca ttggaaaaaa tatcactttt ggtggtacag aacttcgttt
     5221 agaagctaat atctttgatt ttgatggcga catttatgga gaaactattg aaattttctg
     5281 gttaaaaaga atcagagaaa tggtaaagtt taatggtatt gacgatcttg taaaacaact
     5341 taaaaaagat aaagaaattg ctttaaattg gaaaaaagat agtcaaactc tttaattttt
     5401 tgtataatag tctctagtaa atacaagagg cggttatatg attaccttat ttttatcacc
     5461 cagctgtact agttgtcgaa aagcgcgtgc ttggctaagt aagcatgagg ttgcttttga
     5521 agagcataac attattacaa gtccactcaa taaagaagaa ctattacaaa ttctatcgtt
     5581 tacagaaaat ggtacagaag acattatttc cacacgttca aaagtctttc aaaaacttgc
     5641 tattgatgta gacgaattat caacgtcttc attaatggaa ttaatttcag aaaatccaag
     5701 tcttttacga cgcccaatta ttttagacaa aaaacgtatg cagattggtt ttaatgagga
     5761 tgaaattcgt gccttcttac cgcgtgacta tcgtaaacaa gaactgaaac aggccactat
     5821 tcgtgctgaa attgagggaa aacatgatta gtagtaatta tagttatcct cttgatcctt
     5881 catggaatac ggaagatatt actaaggtac ttcgcttttt aaatcaagtt gaacacgcct
     5941 atgaaaatag tattaaagtt gatgatttgc tagatagcta taaagaattt aaaaaagttg
     6001 ttaaatccaa ggcgcaggaa aaacaaattg accgtgaatt ccagagaact agcggttatt
     6061 ccacttatca agctgttaaa gcagcacaac aacaagcgaa aggatttatt tcacttggac
     6121 gctaaatttg atttcgctaa acaattagtg tataaagctg gtcaattcat taaatctgag
     6181 atgcaaaata cttttgatgt tgaggaaaag tctcgatttg atgatttagt gacaagtctt
     6241 gataaaaaaa cacaaaaatt attaattcaa gaaattattc aacattaccc agatgataat
     6301 atacttgccg aagaagatga tgtgcgctca cccattgcac aaggtaatgt ttgggtttta
     6361 gaccctattg atggaactgt taattttatt gttcaaaaag ataactttgc tgttatgtta
     6421 gcttattacg aagaaggcgt tggacaattt ggtatcatct acgacgttat ggctgatatt
     6481 ctttacagcg ggggaggaca ttttgatgtc tatgctaacg ataaaaaaat tgttcctttt
     6541 caagagtgtc ctttagaacg ttgcttactt ggagtaaatt ctgctatgta tgcagaaaat
     6601 gattgcggta ttgcccattt agcttctgaa accttaggtg ttcgtatcta tggtggtgct
     6661 ggaattagca tggcaaaagt catgcagggc aaattattag cttatttttc gtatattcaa
     6721 ccttgggatt atgctgcagc aaaaatcatg ggggagacat taggatttac cttattaacg
     6781 cttgacgggg aagaacccaa ctattcaaca agacaaaaag taatgttcct accaaagtcg
     6841 aaattaaatc ttatccaatc ctaccttacc aaaaaataaa cttaaaacct tctaattaaa
     6901 tagaaggttt ttttgcacgc ccgtgattat gtagtataat agaaacatga aattaccgaa
     6961 tgaatttatt gaaaaatatc agactatact taaagatgaa gcagaagctt tttttgacag
     7021 ttttgaacaa aaacccatat cggcatatcg caccaatcct ctcaaagaaa agcaacttga
     7081 ttttccaaat gccatcccaa gtaccccttg ggggcattat gggaaaatat caggaaaatc
     7141 aatagaacac acaactggcc ttgtttattc tcaagaacct gctgctcaga tagtggctca
     7201 gattgctgaa ccacaagaag gaatgaaagt gctagattta gcagctgcac ctggtggaaa
     7261 aacaacacac cttctatcat atttaaacaa tactggtcta ttagttagta atgaaatttc
     7321 aaataaacgt agtaaaattt tagtcgaaaa tgtcgaacgt tttggtgcta gaaatgttat
     7381 cgtgactaat gaaagctccc aacgactagc caaatgtttt aattcttttt ttgatcttat
     7441 tgtttttgac ggtccgtgtt ctggtgaggg aatgttccgc aaagatcctc aagcaataca
     7501 atattggcac aaagattacc ccactgaatg tgcacagtta caaagagata ttttaaaaga
     7561 agcaatcaaa atgctagctc atggtggtat acttgtatac tctacttgta catggtcacc
     7621 agaagagaac gaagaagtgg ttaattggct ccttcaagag tatgattact tagaacttgt
     7681 tgatattcct aaactaaatg ggatggttga agggattaat gtaccacaag ttgcaaggat
     7741 gtaccctcat cattttcaag gtgaaggaca attcgttgca aaactgagag atactagatc
     7801 taaggaagca cagaaaatta agccaaaagc acagaaaata aataaaatgc agttacaatt
     7861 gtggcaacaa tttgcacaag accatttaaa gatagacttg aacggtgttt tagatgtttt
     7921 cggcgaccaa ctctatcttc tacctaatgg tctccccgat ttgtctaaat taaaaatagc
     7981 acgcaatggt ctccatctag gcactttcaa gaagaatcgt tttgagccat cgtttgcttt
     8041 aggaatggca cttagcgaac atgacctagt acagtctatt gaaattgata tagaacagtt
     8101 tgaggtgtac gtatctggaa atgtagtcaa actagacaag actgttccaa acggttggta
     8161 tcaaattctt gtaaaaggca atggattagg ttttgcaaaa gtgacaaata atactcttaa
     8221 aaattattac cctaaaggac tacgatttca gacataactg ttattgaata cttttaataa
     8281 aaaaggaaaa agaaatactt ttcaaaccct agaaacttat ggtactatgg gacagtgatg
     8341 tgtgttttta ctatttgtaa atcattaaac actcaatgat tttagtaata tatatcaact
     8401 actaaatatc aaaatttatt caaaagaata ggaaaatgaa aatgaaaaaa aacatttttt
     8461 tgttaacctt gctagccacc gtcttattga cgctgtctgg ttgtgctaat tggattgata
     8521 aaggtcaatc aatcaccagc gttggttcaa ccgccttaca gccacttgta gaagctgcag
     8581 cagatgagtt tggtaagaca aacctcggta agactatcaa tgtccaagga ggaggctcag
     8641 gaacaggctt atcacaagta cagtctggag ctgttcaaat tggtaatagc gacttatttg
     8701 cagaagaaaa agatggtatt gatgctaaga aattgaaaga ccatcaagtc gcagtagctg
     8761 gattagctgt tattgttaat aaaaaagtta atgttaaaaa tctaaccacc caccaacttc
     8821 gtgatatttt tgcaggaaaa atcaagaatt ggaaagaagt gggaggacaa gatttagata
     8881 tttccatcat caatcgagca gcaagttctg gatcccgtgc aaccttcgat aatactatta
     8941 tgggaaatgt tgctcctatt caaagccaag aacaagattc aaatggtatg gtgaaatcaa
     9001 ttgtttctca aacacctggt gcgatttcat acctcgcttt tgcatacgtt gataaatctg
     9061 tcggaacatt aaaactaaat ggttttgcgc caacagctaa aaatgttact accgacaact
     9121 ggaaactttg gtcatatgaa catatgtata caaaaggtaa tgaaacaggt ctaacaaaag
     9181 aattcttaga ctacatgaaa agtgataaag ttcaaagtag tatcgtacaa catatgggat
     9241 atatctctat taacgacatg aaagttgtta aagatgctga aggtaaagta accccaaaat
     9301 aaaacttaat cacatctaga taaagcatct tgatcctagg aggaattatg aaaaatcaag
     9361 aattagctaa aaaattaaca tctccttcaa aaaactctcg attagaaaaa tttggtaaag
     9421 gaataacttt tctttcgctt gccctaatcg tcttcattgt tgcgatgatt ttgatttttg
     9481 ttgctcaaaa aggactatct acattttttg ttgatggcgt taaattaaca gacttcttat
     9541 ttaatacaaa atgggaacca agtgctaagt catttggtgc ttttccaatg atagctggat
     9601 catttattgt tactattcta tcagctatca ttgctacacc atttgctatc ggtgctgccg
     9661 tatttatgac tgaaatctca cctaaatatg gttctaaaat tttacaacct gcagttgaat
     9721 tactagttgg gattccttct gttgtttacg gttttattgg tttacagatt attgttccct
     9781 tcgttcgctc tatttttggg ggaacaggtt ttggcatttt atctggggtc tgtgtcctct
     9841 tcgtcatgat tctaccaact gttactttca tgacagtaga tagccttaga gctgtgccac
     9901 gtcactataa agaagcaagt ttagccatgg gggcaactcg ttggcaaaca atttggcgtg
     9961 ttatattaaa cgctgcacgt ccaggaattt tcactgcaat tgtttttggt atggcacgtg
    10021 catttggtga agctctagca attcagatgg ttgtcggaaa ctctgcaatt ttaccaacgt
    10081 cattgacaac accagcagca acattaacgt ctgttctaac aatgggtatt ggtaatactg
    10141 taatgggaac tgtacaaaac aatgtcctct ggtcgctagc cttagtactt ttaattatga
    10201 gtttagcatt taatacagtt attaagttaa ttacaaggga aggaaagaaa aactatgaac
    10261 gctaaaaagg ctgacaaatt agcaactaca atactctata gtattgcagc tatcatcgtt
    10321 acaatcctcg cctctctttt aatttttatt cttgttcgcg gattgccaca cgtatcttgg
    10381 tcatttttaa ctgggaaatc atcatcatat gaagcaggag gcggtattgg aatacaatta
    10441 tacaattcct ttttcctact tattgttact ctgattattt ctattcctct atctttagga
    10501 gctggaatat acctctcaga atatgctaaa aaaggacgtt taacaaactt tgttagaaca
    10561 tgtatcgaaa ttttgtcatc tcttccttct gttgtagttg gtctgtttgg ttacctaatc
    10621 ttcgttgttc aatttcaata tggcttctca attatctcag gtgccttagc acttactgtt
    10681 tttaacttac cccaaatgac acgtagtgtt gaagatagcc ttcaaaatgt acaccacaca
    10741 cagcgtgaag caggattggc cctaggtatt tctcgttggg aaacggtcat ttacgtcgtt
    10801 gtccctgaag ctctgccaag tattgtaact ggtgttgtgc tagcttcagg acgtattttc
    10861 ggtgaggcag cagcacttat ttatacagct ggtcaatctg ctccagcact tgattggtca
    10921 aattggaacg tccttagtgt tacaagtcca atctcaattt tccgtcaagc agaaacttta
    10981 gctgttcata tttggaaagt taatagcgaa gggacaatcc ctgatgcaac acaagtatct
    11041 gccggtagcg ccgcagtctt attggttgtt attcttattt tcaacctttc agctcgctcg
    11101 atcggtaaga aacttcattc gaaattaacc tcgtctaatt aaaaggagta gtaatggcag
    11161 aatataattg ggacgaacgc catatcatta ccttccctga agaaaattca gctctcacca
    11221 caaaagattt acacgtttac tacggtgaga aagaagctat caagggaatt gatatgcaat
    11281 ttgaaaaaaa taaaattaca gccttaatcg gcccatctgg atgtggaaaa tcaacatatc
    11341 ttcgtagctt aaatcgtatg aatgatacga ttgatattgc acgcgtgact ggacaaatca
    11401 tgtatgaagg aattgatgtt aacgctcaag atatcaatgt ttacgagatg cgaaaacata
    11461 tcggaatggt tttccaacgt ccaaacccgt ttgcaaaatc aatttataaa aacattacct
    11521 ttgcatatga gcgtgctggt gtaaaagata aaaaattctt agatgaagtt gtcgaaacct
    11581 cactaaaaca agctgctcta tgggatcaag taaaagatga ccttcataaa tcagcattca
    11641 ctttatctgg cggtcaacag cagcgattat gtattgcacg tgctatcgca gttaaaccag
    11701 aaattctctt aatggatgaa cctgcttcag cactcgatcc gattgcaact atgcaactag
    11761 aagagacaat gtttgaactc aagaaaaatt acactataat tatcgtaaca cataatatgc
    11821 aacaagcagc acgtgctagt gattatactg catttttcta tcttggagac ttaatcgaat
    11881 acgataaaac gaataacatt ttccaaaatg ctaaatgtca atctactagc gattatgttt
    11941 ctggtcgatt tggatagtga ggtaaaagat gacacaacct attttacaag tcagtgattt
    12001 gtctgtttac tataataaga aaaaagcttt aaaagaagtc tcaatggact tctatcctaa
    12061 tgaaattaca gccttaattg gtccgtctgg ttctggaaag tcaacactct tacgagctat
    12121 taaccgtatg ggagacttaa atcctgaagt cactctaaca ggagcagtga tgtataacgg
    12181 acataatgtt tatagcccgc gcactgatac cgtagaactt cgtaaagaaa ttgggatggt
    12241 ttttcagcag cctaatccat ttcctatgtc agtatttgaa aatgttgttt atggtcttag
    12301 actcaaagga ataaaagaca aagctacgtt agatgaagcc gtagagactt cattgaaggg
    12361 cgcgtctatc tgggacgagg tcaaagatag attacacgac tctgctcttg gtttatctgg
    12421 tggtcaacaa caacgtgttt gtattgccag aacccttgct accaagccta aaattatcct
    12481 tctagatgag ccaacctctg cacttgaccc aatttctgct ggtaaaattg aagagacttt
    12541 acatggtcta aaagatcaat ataccatgtt acttgttaca cgttctatgc agcaagcctc
    12601 acgtatttct gatcgtacag gtttcttctt agacggtaac ttaattgaat atggcaatac
    12661 aaaagaaatg tttatgaatc ctaaacataa agaaactgaa gattacatta caggtaaatt
    12721 cggttaatac catataataa gatcagaaag agaatacaat atgttaagat caaaatttga
    12781 tgaagaatta gataaactgc ataaccaatt ctatgcaatg ggaattgaag ctatcggaca
    12841 aatcaaaaaa acagttcgtg cttttgtcag tcatgatcga gagttagcta aagaagtgat
    12901 agaagatgat gtaacgctta acaactttga aacgaagtta gagaaaaaat cattggaaat
    12961 tattgcactc cagcagcctg tatcacaaga cttacgcacc gttattaccg ttttaaaagc
    13021 tactagtgat gttgagcgta tgggagacca tgcagcagca gttgcaaaag caaccatccg
    13081 tatgaagggc gaggaaagaa tcccagcagt agaattagaa atcaataata tgggaaaagc
    13141 agttaaaaac atgcttgaag aagccttaac agcttatatc aatggtgatg atgaaaaagc
    13201 ttacgaagta gcagcaatgg atgaaatagt tgatgattac ttccgtgata tccaaaaaat
    13261 ggtagttgaa acaattcaaa agcatcctga cgtagcattt gccgccaaag aatacttcca
    13321 agtattgatg cacttggagc gaattggaga ttatggcaaa aatatctgtg aatggattgt
    13381 ttatttaaaa actggaaaaa ttatcgagtt atgatataat tattgcttgt agagtggtaa
    13441 ttgtaagtat agttcgtgcg atagaaaagc tgtaactatc ttgttacagc ttttctatct
    13501 tttctagccc atacaggact agtaatgttt caaatgtttc tgaattagtt aggagttata
    13561 tgaaaactgt agaacatttt gttaccaaat ttgtccctga aaactataac cttttcttgg
    13621 atattaatcg tcaaactaaa accttttcag gtaacgttgc tgtgtctggc gaagccttag
    13681 ataacaatat ctcttttcac caaaaagggt taaccattaa atctgtctta ttagataatc
    13741 aaccattaga tttccaacta gatgaagaca atgaagctat gcatattcaa ttgcatgaaa
    13801 ctggtagcat ggtattagtc tttgaattca gcggtcacat taccgataat atgacaggta
    13861 tgtatccatc ttactataca gttaatggca tcaaaaaaga ggttatctcc acacaatttg
    13921 aaagtcattt tgctcgtgaa gtattcccaa gtattgacga accagaagca aaagcaacct
    13981 ttgatttgag cctaaaattc gatcaaaaag aaggagagat tgctctttca aatatgccgg
    14041 aaatcaatgc tgaacaacgt caagaaacag gtttatggac atttgacacc acacctaaaa
    14101 tgtcctcata tttattagct tttgccttgg gggaattgca tggtaaaaca acacatacca
    14161 aaaacggcac tcttgttggc tcatatgcca caaaagccca tcagttaaat gaattagatt
    14221 tttctttaga tatcgttgtg cgtgttattg agttttatga ggactacttt ggtgtacgtt
    14281 atcctattcc tcagtcactc cacgttgctc tacctgattt ttctgctgga gctatggaaa
    14341 attggggatt agtaacatat cgtgaagtct atcttttagt agatgagaac tctagtgttt
    14401 ccagccgtca gcaagtagca ttagttgtag cacatgaaat agctcaccag tggtttggta
    14461 acctcgttac tatgaaatgg tgggatgatt tatggttaaa tgaatcattt gctaatatga
    14521 tggaatatgt atcaattgat tatattgaac caaaacttaa tatatttgaa gactttcaaa
    14581 caggtggtct accattagct cttaagcgtg atgccacaga tggcgtccaa tctgttcacg
    14641 ttgaggtcaa tcatcctgat gaaattaaca cactttttga tccagctatc gtttatgcaa
    14701 aaggtagccg cctcatgcac atgttacgtc gctggttagg cgatacagat tttgccgcag
    14761 gactaaaaat ttattttgaa aaacatcaat atcaaaatac tattggtcgt gacctttgga
    14821 atgcactttc tcaaacctca ggaaaagatg ttgcagcttt catggattct tggttagagc
    14881 aaccaggata ccctgtgatg gcagccaaaa tagaagaaga tgagctcatt ttaactcaaa
    14941 aacaattctt tattggtgaa cacgaagata agtctcgctt gtggcaaatt cctcttaata
    15001 gtaattggga aggaatccca gagatattaa ccgaggaaac ggtagtaatc cccaatttta
    15061 gtcagttagc tgaaaagaac aaagaaaatg gtgcactgcg tttcaatact gaaaatactg
    15121 cacattacat tactaactat caaggtcaac tcttagagca tatcatttct gatcttcctc
    15181 ttatggataa catttctaaa ctacaaattg tacaagaacg tcatttactt gctgaaagtg
    15241 gtatgatttc atatagtagt ttaatacctt tagtctctct tttgagccaa gagacatcat
    15301 atcttgttaa ctctgctatt aagagtgtta ttgatggttt atccttattc gtccaggaag
    15361 atagccaaga tgaattcgac tttaaagaat ttgttaataa attatcagca tttaatttta
    15421 atagacttgg ctttgaaaaa agagaaggag aaggagatga tagcgaaatg gtccgtcatc
    15481 tatccctaag ccttgctctc tactcagata atgaacatgc tattgaagaa gctcaccata
    15541 tttttaaagc ccacgaaaat aatatcgctg ctataccagc tgcaattcgc ctgttagttc
    15601 ttaccaatga aatgaagcat ttcgaatcca aagaacttag ccacttgtta ttagagactt
    15661 atagtactac taccgatggg aatttcaaac gacaactagc gagtgcttta tctcatacta
    15721 cagatagcaa gacactcaag aaattattgt cagattggaa aaataaagat attgtgaaac
    15781 cacaagactt agctatgagt tggtatgcta ctttccttaa aaattctttt acacaagaaa
    15841 gtgtctggga gtgggcccaa gaaaactggg aatggatcaa agcaactcta ggtggtgata
    15901 tgagttttga taaatttgtg atttatccct caagtagttt caaaacagaa gaacgccttg
    15961 aacaatataa aaactttttc gagcctcaat taagcgatat ggcgattagc cgtaatattt
    16021 caatgggaat taaagaaatt tctgctcgtg tccttttaat cacgaagcaa aaagaagaag
    16081 ttatcaatac tatcaaaaaa tatatctaat aaactcaaat agagattgat ggattattca
    16141 atctctattt ctttgtctgt gtcatgaaat tagttgacat aaaacatact ttatttagga
    16201 tttattaagc aaagtttggt attctaatat taatagttca gataaaatat gtacatcgaa
    16261 aggatatact atgataaaaa ttttattaat tgaagatgac ttgagtcttt ccaattccgt
    16321 ttttgatttc cttgatgact ttgccgatgt catgcaaatt tttgatggag aagagggtct
    16381 ttacgaagcc gaaagtggag tctatgatct gattcttcta gatctcatgt tacctgaaaa
    16441 aaacggcttc caagtgttga aagagctacg tgaaaaaggt attacaaccc ctgtattaat
    16501 aatgaccgct aaagaaagca ttgatgataa gggacaaggt ttcgacttag gtgctgatga
    16561 ttatctaaca aaaccttttt acctagaaga actaaaaatg cgaattcagg ctcttttaaa
    16621 gcgttctgga aaatttaatg ataacagcct aatttatggt gatattcgcg tagacatgtc
    16681 tactaactca acctttgtta atcaaacaga agttgagctc ttaggtaaag agtttgatct
    16741 tttagtctat ttccttcaaa atcaaaatgt tatcttaccg aaatctcaaa tctttgatcg
    16801 tatttgggga tttgatagtg atacaacgat ttcagttgta gaagtctatg tctctaaggt
    16861 tcggaaaaaa ttaaaaggaa cacttttttc agaaaacctt caaactttgc gtagtgtagg
    16921 atatatttta aagcatgtcg aaaccgatta agataagtaa attcaaaaaa aatgtctcag
    16981 atagtcattt tatccatttt ttcacagttt ttagtggtat ttttctggtt atgactgtta
    17041 tcatcctcca agtcatgcgc tatggtgttt attcttctgt tgatagtagt ttaaagtata
    17101 tcagtaccca tcctaaaaat tacatcaata tggttatgtc acgtactgct gcctacctag
    17161 ataactccaa tatcgcaagt gtcaagttaa aacccggcgg tcagacagta gctaatactg
    17221 atattattct ttttacatca gaagaagaag tcattaacta ttttgacgca ttttcaaact
    17281 atcagttttt aaaacctaat aaaaaaaatc taggtggcat tagtgaatta actttaacca
    17341 acatctttgg tcaagatgag acatatcatg ctgtcaccgt aaaagttaat aaccctgctt
    17401 atcctaatgt gacttacatg acagctattg ttaatattga tcaattagta aatgctaaag
    17461 aacgctatga aaaaattatt atatttgtca tgactacttt ttggattatt tcaattggag
    17521 caagtatcta cttagctaaa tgggcacaaa aaccgattat agaaaactac gaaagacaga
    17581 aggcttttgt cgaaaatgct agccacgagc tgcgtactcc cctagctgtc ttacaaaatc
    17641 gcctagaaac gctattcaga aaaccgaatg ccaccatttt agaaaatagt gaaaatatcg
    17701 cttcaagtct agatgaagta cgaaatatgc gtattttaac cactaatctt ttaaacttag
    17761 caagacgtga tgatggaatc aaaccagaat tagctgttat taagcctaca ctttttgata
    17821 gtatctttga gaattatgat ctaatcgccc aagagaatgg taaaaatttt actggacata
    17881 atatgatcca agatagtttc aaaacagata agacactgct aaaacaactc atgactattt
    17941 tatttgataa tgctataaaa tatactgaca atgatggtag cattgacttt accatatctg
    18001 aaactgataa gtatctcttt ttagaaatcg ctgataatgg accaggaatt tcagaggagg
    18061 ataaagtcag aatttttgat cgtttttacc gtgttgacaa agcaagaaca cgacaacaag
    18121 gagggtttgg cctcggcctt tctttagctc aacaaatcgt taactcactg cgaggtaata
    18181 ttacagtaat tgataataaa cctagaggca gtatttttaa gataaaacta ccaaaagtaa
    18241 aaggttaggc catgttagtc ctaacctttt aaattttaaa gtggtcttgc atacttgata
    18301 tagcaagctg ataggaaatt tggtataata aaagctatgg aaattgaaaa aacaaatcgt
    18361 atgaatgccc tttttgagtt ttatgcggca ttattaacag ataaacaaat gaattatatc
    18421 gagttatact acgccgatga ctacagcctt gctgaaatag ctgaagaatc cggtgttagc
    18481 cgacaagctg tctatgataa tatcaaacga actgaaaaaa ttcttgaggc gtacgaaatg
    18541 aaactacata tgtattcaga ttatattgtc cgtagtcaga tttttgatga cattctagaa
    18601 aaatataccg atgatgcctt tttacaagaa aaaatttcca ttctaagtag cattgataat
    18661 agagattaaa tgaggagaaa taatttatgg ctttcgaaag tttaacagag cgcctgcaag
    18721 gagtttttaa aaatattcgt ggtaagaaaa agttatctga aaaagatgtg caagaagtca
    18781 ccaaggagat tcgtctagct cttttagaag ctgacgttgc tcttccagtt gttaaaacct
    18841 ttattaaaca tgttcgtgaa cgtgctgttg gacatgaaat cattgataca ctagatccta
    18901 cgcaacaaat tgttaagatt gtcaatgaag agctaacaga tttattaggg gctgagacct
    18961 ctgaaattga aaaatcccct aaaattccaa ccatcattat gatggttggt ctacaaggtg
    19021 ctggtaaaac aacttttgcc ggtaagttag caaacaaact aatcaaagaa gacaatgctc
    19081 gtcctatgat gatagcagct gatatttatc gaccagctgc tattgatcag ttaaaaaccc
    19141 ttggtagcca gattaatgtt cctgtctttg atatgggaac taaccattca gcagttgaga
    19201 ttgtcactaa aggtttagaa caggctcgtg aaaatcgtaa tgactatgtt cttattgata
    19261 cagccggacg cctacaaatt gatgcaaccc ttatgcagga attgcatgat gtcaaagcca
    19321 tagctcaacc taatgaaata ctccttgtcg ttgacagcat gattggacag gaagctgcaa
    19381 atgtcgctga agaatttaac cgtcagctca gcattagtgg tgttgttctg acaaagattg
    19441 acggtgatac acgcggtggt gctgcccttt cagtacgcga aattactgga aaaccaatta
    19501 aatttacagg gacaggggaa aaaatcactg atattgaaac tttccatccc gatcgtatgg
    19561 caagccgtat attggggatg ggagacctgc taacacttat tgagcgcgct agtcaagaat
    19621 acgacgaaaa acgttcaatg gaattagcgg agaaaatgcg tgaaaacacc tttgacttca
    19681 acgattttat tgatcaatta gatcaagttc aaaatatggg gccaatggaa gatctcctta
    19741 aaatgcttcc aggaatggct aacaatccag caatgaaaaa cttcaaagtg gatgaaaacg
    19801 aaattgctcg caaaagagcc attgtgtcat caatgactcc agaagagaga gaaaatccag
    19861 atctcctcaa tccaagtcgt agacgtcgta ttgctgctgg atcaggtaat actttcgttg
    19921 atgttaataa attcattaaa gattttaacc aggcaaaaca aatgatgcaa ggcgtgatgt
    19981 ccggtgatat gaataaaatg atgaaaaaaa tgggaattga tcctaataac ctccctaaag
    20041 atatgcctgg aatggacgga atggatatgt ctaatttaga aggtatgatg gggcaaaatg
    20101 gcatgcctga cttatctagt ttaggtggcg atatggactt cagccaaatg tttggtggtg
    20161 gcttaaaagg aaaagtagga gcctttgcag ctaaacaatc aatgaaacgt atggctaata
    20221 aaatgaagaa agctaagaag aaacgcaaat aaaaatgtct atttaaatag acatttttat
    20281 ttttgtctgc tataatagac aaaaaggtgg tgatatttat gtatttagca ttaatcggtg
    20341 atatcattaa ttcaaaacag atacttgaac gtgaaacttt ccaacagtct tttcagcaac
    20401 taatgaccga actatctgat gtatatggtg aagagctgat ttctccattc actattacag
    20461 ctggtgatga atttcaagct ttattgaaac catcaaaaaa ggtatttcaa attattgacc
    20521 atattcaact agctctaaaa cctgttaatg taaggttcgg cctcggtaca ggaaacatta
    20581 taacatccat caattcaaat gaaagtatcg gtgctgatgg tcctgcctac tggcatgctc
    20641 gctcagctat taatcatata catgataaaa atgattatgg aacagttcaa gtagctattt
    20701 gccttgatga tgaagaccaa aaccttgaat taacactaaa tagtctcatt tcagctggtg
    20761 attttatcaa gtcaaaatgg actacaaacc attttcaaat gcttgagcac ttaatacttc
    20821 aagataatta tcaagaacaa tttcaacatc aaaagttagc ccaactggaa aatattgaac
    20881 ctagtgcgct gactaaacgc cttaaagcaa gcggtctgaa gatttactta agaacgagaa
    20941 cacaggcagc cgatctatta gttaaaagtt gcactcaaac taaaggggga agctatgatt
    21001 tctgattttt taagggataa tccaatccta actttattat tttgtgcaca ttttttagct
    21061 gactttcagt ggcaaagtca atccttagca gatagtaaat cacatagttg gaggggactc
    21121 tggagacatc tcttgattgt tttcctcccc ctagcagctt taatgatatt gataccagaa
    21181 actactttgt taaatttaag catatgggga agtcatattg ttattgatag tataaagaaa
    21241 ttgagttacc cttgggtaga ggaagggcat ttccaaaaag cagcttttat tatagaccaa
    21301 ctagcccact atacttgcat tatcgttttt taccatgcgc ttcctacata tttaccgcct
    21361 aatcattggc ttttgcctat caagcacttt attgtcatag ctctggtttt tatcattatc
    21421 accaaaccca ttaatattgt gtttaaaatt ttctttaata aatttcaagc taaagaatta
    21481 tcatcattat taacacaaga aaaaacaaaa ataatgaaag aaaaatctga agatcatgaa
    21541 gaaacaattg aaggagcagg agctatgatt ggtaatttag agcgtctaat tatggctata
    21601 ttactaatct caggacagta tgctgctata ggtcttgttt ttactgcaaa atccattgca
    21661 cgttacgata aaatttctaa aagtcaggtt tttgccgaat attatttaat tggatcattg
    21721 ttctcaatta taagtgtttt gataactcat tggttacttt tggtatagaa aagcgctaga
    21781 aatatcctaa cgcttttctt ttattctgtc ttataagcat aagtaaaatc aacattcaaa
    21841 ccagttgaat ttcttaccaa cccctttaat ttagaatttt ggagtccttt tttccctagg
    21901 taataaactg ggttataata tgattgatca aataaagctt tttcagcagt tttgtaatca
    21961 ttagcggcat ctcctggttt caaggcgtca gtagtaattg ccttttgata ggcattgtca
    22021 tactctttag aagaaaattt accataatta tacgcagaat tagtcgtgaa taacccatag
    22081 aaagttgatc cttctggata atctcctccc catgagaaga gcacgacatc aaaattttca
    22141 ttttttgcat cttcaaggcg ttgcttgaaa gtaacaaatt tttcttcaac agttagacca
    22201 ggcaaagcac tctcccatgt agatttcaca tagtcaacag catttttagc tgctggagag
    22261 tccgaatcag ctgtgattgt taacttgaga gatgactgac caacctcagc aagaccttct
    22321 ttaaaaagtt tttgagcttt acttgtatta tacgtataac ctggggcagt atacttggaa
    22381 agatcctcac cattaggtaa tttggctaat tttttaggga ccagagattc agcaggagtt
    22441 gaacctgtat caactgccgc tttaacaaca cctttacgat cagtcgctaa attcaaagct
    22501 tgcctaattt tctgatttgt caatgcttta accgaaccag tttggttata aataatatat
    22561 gtagtacgtg catcagacgc atctactaca tctttattat ttttattagc ttgataaata
    22621 gcagaagttc ctgatatttc agcaaaatca atctgacctt gcttgtacat ttgaactgca
    22681 gtatctggtt ttttgatagt ttgaactatg acactatttg ttttaacatg tttagaatcc
    22741 caataatatt tatttttgac taatttaaat ttaccatttg aaccattcca atccttaaca
    22801 aggtaaggac ctgagtaaat ttgatttttt gatgttgtac cataatcttt accaactttt
    22861 tcaacatacg attgtttttg aggcataaaa ttagagaagg ctaaataata tttaaattga
    22921 gggcatggac ttgtcaattt aaaggtcact tgattgcctt tagctgttac accaagttta
    22981 tttaaatctt taatatctcc agaattaatc ttatcagcat ttaaaaggtg cgattctgtc
    23041 gccaagtaag catattcaga tgcagtttta ggatcaacta ttctccgcca tgtgtaaaca
    23101 aaatcttcag cgcttagttt gcttccatct gaccatttta aattatcacg aagtgtagca
    23161 gtataagtaa gaccatcaga agaaacggat acttttttag ctaaatcggg ttttggtttg
    23221 ccctctttat caattctaag taaattgctg cctgaattac ctatagctaa gtttgaataa
    23281 gcatctgtat tctttgaaat gtccaatgta ctgatttcag taggaatata ccaattaata
    23341 actttattat ctccagagtt ctttgactca ttaccacagg caaccagggt aactgtggtg
    23401 gctgttagag tcgccaaaga tatacccaaa aacttaagtt tttttctatt aaaactagac
    23461 ataataccag tcctcctaaa ccttctataa agatatagga catattatat cataaaattt
    23521 caagaaagaa agaaatattt caaattttct gatttttata agacatagta gatctaatat
    23581 tgagaaaact agtatatttt aaagtagaat ttaactttgg ttattaactt gcaaatat
//
LOCUS       DATRNK010000027        23175 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.027, whole genome shotgun sequence.
ACCESSION   DATRNK010000027 DATRNK010000000
VERSION     DATRNK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23175
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.027"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..2538)
                     /locus_tag="VBC52_001072"
     CDS             complement(<1..2538)
                     /locus_tag="VBC52_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO6882668.1"
                     /translation="MNRKKTVIISALSVALFGTGVGAYQLGSYNAQKSDNSVSYVKTD
                     KSDSKAQATAANKTPDQISKEEGISAEQIVVKITDDGYVTSHGDHYHYYNGKVPYDAI
                     ISEELIMKDPSYVFNKADVINEVKDGYIIKVNGKYYLYLKEGSKRTNVRTKEQIQKQR
                     EEWSKGGSKGESGKHSSAKTQALSASVREAKASGRYTTDDGYVFSPTDVIDDMGDAFL
                     VPHGDHFHYIPKADLSPSELSAAQAYWNRKTGRSGNSSKPSNSSSYIHASAPSGNVST
                     GRHANAPISIPRVTHANHWSKPAGNHATAPKHHAPTTKPINKDSALDKMLKRLYAQPL
                     SARHVESDGLVYDPAQVNAFTAIGVSIPHGNHFHFIHYKDMSPLELEATRMVAEHRGH
                     HIDALGKKDSTEKPKHISHEPNKEPHTEEEHHAVTPKDQRKGKPNSQIVYSAQEIEEA
                     KKAGKYTTSDGYIFDAKDIKKDTGTGYVIPHMTHEHWVPKKDLSESELKAAQEFLSGK
                     SEANQDKPKTGKTAQEIYEAIEPKAIVKPEDLLFGIAQATDYKNGTFVIPHKDHYHYV
                     ELKWFDEEKDLLADSDKTYSLEDYLATAKYYMMHPEKRPKVEGWGKDAEIYKEKDSNK
                     ADKPSPAPTDNKSTSNSSDKNLSAAEVFKQAKPEKIVPLDKIAAHMAYAVGFEDDQLI
                     VPHHDHYHNVPMAWFDKGGLWKAPEGYTLQQLFSTIKYYMEHPNELPKEKGWGHDSDH
                     NKGSNKDNKAKNYAPDEEPEDSGKVTHNYGFYDVNKGSDEEEPEKQEDESELDEYELG
                     MAQNAKKYGMDRQSFEKQLIQLSNKYSVSFESFNYINGSQVQVTKKDG"
     gene            complement(2565..3488)
                     /locus_tag="VBC52_001073"
     CDS             complement(2565..3488)
                     /locus_tag="VBC52_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEO6882669.1"
                     /translation="MKRIRKSLIFVLGIITLICLCACTKQSQQKNGLSVVTSFYPVYS
                     ITKAVSGDLNDIKMIRSQSGIHGFEPSSSDVAAIYDADLFLYHSHTLEAWARRLEPSL
                     HHSKVSVIEASKGMTLDKVHGLEDVEAEKGVDESTLYDPHTWNDPVKVSEEAQLIATQ
                     LAKKDPKNAKVYQKNADQFSDKAMAIAEKYKPKFKAAKSKYFVTSHTAFSYLAKRYGL
                     TQLGIAGVSTEQEPSAKKLAEIQEFVKTYKVKTIFVEEGVSPKLAQAVASATRVKIAS
                     LSPLEAVPKNNKDYLENLETNLKVLVKSLNQ"
     gene            complement(3743..4186)
                     /locus_tag="VBC52_001074"
     CDS             complement(3743..4186)
                     /locus_tag="VBC52_001074"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEO6882670.1"
                     /translation="MKIIIQRVSQASVSIEDDVVGSIEKGLVLLVGIAPEDTTEDIAY
                     AVRKITSMRIFSDDDGKMNLSIQDIKGSVLSISQFTLFADTKKGNRPAFTGAADPVKA
                     NQFYDIFNQELANHVSVETGQFGADMQVSLINDGPVTIVLDTKNK"
     gene            complement(4196..6412)
                     /locus_tag="VBC52_001075"
     CDS             complement(4196..6412)
                     /locus_tag="VBC52_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEO6882671.1"
                     /translation="MVKEINLTGEEVVAITSQYMSETDVAFVKFALNYATAAHYYQAR
                     KSGEPYIIHPIQVAGILADLHLDAVTVACGFLHDVVEDTEITLDEIETDFGKDVRDII
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLSFRYLNETEFYKISHMMSEKRREREELV
                     DIIVDKIRSYTEEQGLYGDIYGRPKHIYSIYRKMRDKKKRFDQIYDLIAIRCIMETAS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKEMHQV
                     AEFGVAAHWAYKKGITSKVNQAEQSVGMGWIQELVELQDESKDAKDFVDSVKEDIFTE
                     RIYVFTPNGAVQELPRESGPIDFAYAIHTQVGEKATGAKVNGRMVPLTAKLKTGDVVE
                     IITNPNSFGPSRDWIKIVKTNKARNKIRQFFKNQDKETSINKGRELLVDYFQEQGYVP
                     NKYLDKKHIEEILPRVSVKSEEALYAAVGFGDLSPISIFNKLTEKERREKERAKAKAE
                     ADELINGGEIKTDKRDVLKVKSENGVIIQGASGLLMRIAKCCNPVPGDLIEGYITKGR
                     GVAIHRSDCQNLKSQENYEQRLIDVEWDDDGSKKEYMAEIDIYGLNRSGLLNDVLQTL
                     SNATKLVSTVNAQPTKDMKFANIHVSFGISNLAQLTTVVDKIKIIPDVYSVKRTNG"
     gene            6672..9074
                     /locus_tag="VBC52_001076"
     CDS             6672..9074
                     /locus_tag="VBC52_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HEO6882672.1"
                     /translation="MSKHYFSKSVFALTVLTATATSGLAVQAEDIVTTPSSTATKVES
                     TTPTSTIAEEKSNVTSTPTAITDASTATSTNTTANNQNPQPVATETTTGDLKPIEGEK
                     VDLRIMSTTDLHTNLVNYDYYQDKESQKIGLAKTAVLIEEAKKENPNTVLVDNGDVIQ
                     GTPLGTYKAIVKPVAENEEHPMYQAMNALGYDASTLGNHEFNYGLDYLKKIIATANLP
                     ILNANVLDFKTHQPVFKTYDIITKTFKDSTGRAVALNIGITGIVPPQILNWDKANLEG
                     KVIVKDSVKAIEEIVPTMRAKGADVILVLSHSGIGDDRYEEGEENVGYQIASIKGVDA
                     VVTGHSHAEFPSGNGTGFYEKYTGVDGINGKINGTPVTMAGKYGDHLGIIDLGLSYTN
                     GKWQVSESSAKIRKIDMNSTTADERIIALAKEAHDGTINYVRQQVGTTTAPITSYFAL
                     VKDDPSVQIVNNAQRWYVANELKGTPEANLPLLSAAAPFKAGTRGDATAYTDIPAGPV
                     AIKNVADLYLYDNVTALLKVTGADLREWLEMSAGQFNQIDPNNKAPQNIINTEYRTYN
                     FDVIDGLTYKFDITQPNKYNKDGKVVNSQASRVRDLMYNGKPVADKQEFMIVTNNYRA
                     SGTFPGAKNATMNRLLNLENRQTIINYIISEKTINPTADNNWGFTESIKGLDLRFQTA
                     DKAKNLVTNSKDIQYIASSTKDEGFGDYRFVYTEQEKVDLTEPSHQEKLSQIEKQKIH
                     HMVATAYTKAKASPNITGTVYTQQKEKTLPKTGQEKQSYLAFFGVAILGLVGSLLKKK
                     QI"
     gene            9231..9686
                     /gene="nrdI"
                     /locus_tag="VBC52_001077"
     CDS             9231..9686
                     /gene="nrdI"
                     /locus_tag="VBC52_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO6882673.1"
                     /translation="MPKLTLVYISLSGNTQSFVKRLSEQLSTWHNCQVSTINIKEQNH
                     QTFPVDQPFVALLPTYLEGGNGIDNGDQEILTIPLGDFIAAHNNVKNCIGIVGSGNRN
                     FNNQYCLTAKQYSERFGFPMLGDFELRGTSSDVERIANIIVETLSNFKA"
     gene            9697..10734
                     /locus_tag="VBC52_001078"
     CDS             9697..10734
                     /locus_tag="VBC52_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEO6882674.1"
                     /translation="METTLNYIKTLTSIPSPTGFTQTIMTYIIKELESFGYSPIRTNK
                     GGVMVSLKGKNDTKHRMITAHLDTLGAMVRAIKPDGRLKIDLIGGYTYNAIEGENCTI
                     HLSKNGKEISGTALIHQTSVHVYKDAGTAERNQTNMEIRLDEKVTTADETRALGIQVG
                     DFISFDPRTIVTDSGFIKSRYLDDKVSAGILMELLSVYKKEDIQLPYTTHFYFSAFEE
                     LGHGANSSIPNETVEYLAVDMGAMGDDQETDEYTVSICVKDASGPYHYELRQHLVSLA
                     ENNNIPYKLDIYPYYGSDASAAMRAGAEVKHALLGAGIESSHSYERTHIDSIQATELL
                     VDAYLKSNMVN"
     gene            10734..11231
                     /locus_tag="VBC52_001079"
     CDS             10734..11231
                     /locus_tag="VBC52_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO6882675.1"
                     /translation="MVATLIIIRGNSASGKSTIAKQLQAELGEDTLLLSQDYLRREML
                     GTKDGENTTTIPLLINLLNYGYHNCSYIILEGILRSDWYTPVWKHILKHNPNNTYAYY
                     YDLSFQETVKRHSTRLKSLEFGEDSLARWWLEKDFLKEIPEKILTKSMSLEDVKKLIM
                     TDVLP"
     gene            complement(11453..12490)
                     /locus_tag="VBC52_001080"
     CDS             complement(11453..12490)
                     /locus_tag="VBC52_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO6882676.1"
                     /translation="MKEKQSKRLIYILLVVPIIFISIFTYSISQPSKLLPPKELVILS
                     PNSQAILTGTIPAFEEKYGIKVKLIQGGTGQLIDRLSKEGKQLKADVFFGGNYTQFES
                     HKALFESYVSKNVHTVIPDYIHPSDTATPYTINGSVLIVNNELAKGLTIKSYEDLLQP
                     SLKGKIAFADPNTSSSAFSQLTNILLAKGGYTNPKAWNYVKKLQHNINAIKSSSSSEV
                     YQSVAEGKMIVGLTYEDPSVNLQKSGANVSIVYPTEGTVFVPSSVAIIKNAPSMKEAK
                     LFINFMLSLDVQNAFGQSTSNRPIRKDAQTSNGMKALKDIATLKEDYRYVTKHKGQIL
                     KTYNRIRRNAD"
     gene            complement(12502..13275)
                     /locus_tag="VBC52_001081"
     CDS             complement(12502..13275)
                     /locus_tag="VBC52_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO6882677.1"
                     /translation="MYRLLIVEDEHLIRKWLRYAIDYQSLNILVVGEAKDGKEGAQLI
                     QEEQPDIVLSDINMPIMTAFDMFEATKGQSYAKIILSGYADFPNAQSAIHYGVLEFLT
                     KPLEKQALIDCLKTIMARIEEHKEKHLQEHTELYLPLPQANDQVPEVIKDMLAWIHSH
                     FHGKIVISQLAHDLGYSESYLYTVTKKHLHITLSDYINQYRINQAIQLMFREPDLMVY
                     QIAEAVGIYDYRYFDRVFKKYLGQTVKAFKEEHIFKQMD"
     gene            complement(13279..14928)
                     /locus_tag="VBC52_001082"
     CDS             complement(13279..14928)
                     /locus_tag="VBC52_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6882678.1"
                     /translation="MRGYRMEERFKKRLQDDISKHFSRQSLILSLLLIALFVLFSLAP
                     QQIGLYKDVNSVSYSYKQLIQKHDTLLDDLGKNSLKPFVSGHLGSADLSKQYYHLRNH
                     LQSQTELLVFSPNQELLFASNSHLGNFFSKSIYISEVLDKAKINQRLLKIIVDSEGGH
                     YLALIKPIIVNKKVSGYAFLLMNGKDFLLPTKAINSDLIIADQLNNSFTFTNRDFISS
                     SLDKVDSQFLTRYFSFHDHRAFVVRKVALQDNILLYMYRPLIPVTLVVLFSLVSSVII
                     FVILRRKSRVLADRIAVKNSSAINQMVLDMDAISRQEKSSIELDSQDEFQYLSVQINQ
                     MVSRLKDLHEKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETVLITSHYDSQLTERIV
                     IQLTKLLRYSLSGSTEAAVLKDDLAIIESYLLINQVRFEELTYTISVSPELEHMRVPK
                     LFLLPLIENAIKYGLKERHDVAINIDIWQDSDGIWFTVSNNGSGISLARQKAIRTMLR
                     STHSHHGLINSYRRLQYQFSTVLLEFTKTDDAFRVSYIVKE"
     gene            complement(15066..15893)
                     /locus_tag="VBC52_001083"
     CDS             complement(15066..15893)
                     /locus_tag="VBC52_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6882679.1"
                     /translation="MKSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASTGSAMGAVVFLLLYNIPAFLIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQSYLDQLFVGVVPLLVTILAFWLLRKKVNINWIMFGIMVLGIVLGLLGIC"
     gene            complement(15890..16699)
                     /locus_tag="VBC52_001084"
     CDS             complement(15890..16699)
                     /locus_tag="VBC52_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO6882680.1"
                     /translation="MIMPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGMLLGDI
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISNIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNSFVHHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDIELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            complement(16715..17206)
                     /locus_tag="VBC52_001085"
     CDS             complement(16715..17206)
                     /locus_tag="VBC52_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6882681.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEESIALFKDPRATDKRIFVIVNSVKDACTIAKNITDLEAVN
                     VANVGRFDKSDPATKVKLTSSLLLNPEELEAAKELASLPDLDVFNQVLPSNTKVNLSQ
                     LVK"
     gene            complement(17224..17649)
                     /locus_tag="VBC52_001086"
     CDS             complement(17224..17649)
                     /locus_tag="VBC52_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEO6882682.1"
                     /translation="MKRKFLIGSHGKLASGLQSSIDILTGEGQEIQTIDAYIDDSDYT
                     KSIVEFIDEIAPDEQGLIFTDLLGGSVNQKMATAVMNSGKNNVFLITNSNLATLLSLL
                     FLKPEEELTKEEIVTVINESQVQLVDLSFKASSEDDFFD"
     gene            complement(17896..18930)
                     /locus_tag="VBC52_001087"
     CDS             complement(17896..18930)
                     /locus_tag="VBC52_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO6882683.1"
                     /translation="MDNKQHNTSMISLLQYLFSILVILVHSGRLFSQDVIHFTFKSFL
                     GRMAVPYFLICTAFFLRGRIQQGLCNHSYFRKLIKKYSMWTIIYLPYGYFFFESLNIA
                     KIYLLPGFIVALLYLGMLHTLWYIPAVILGWIIIQGLLKYVGTRGTFITVVVLYCIGA
                     VETYSVFVQSTKFYPLMSTYMSIFQTTRNGLFYTPVYLLAGYLLYDYFNTDLFTKSRG
                     LKYILFLLLLALENVLIYFNQGLDKNFFLLAPLCAVFLFNWSIRTSLFKDYRLSPLKQ
                     LSVYYFFLHPLFVGIVSYCLKSTSLVAHHQGKVIFVVTLALTHACALLIIKVVNKIPY
                     QKIIPLSLSK"
     gene            complement(19159..20313)
                     /locus_tag="VBC52_001088"
     CDS             complement(19159..20313)
                     /locus_tag="VBC52_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEO6882684.1"
                     /translation="MISIFPFELKKLLQRKAALGAFIIFLLAIFGLFYQHFFVGQISG
                     YSADKIHGREAVAINRRIAEKYSGDLSEERVTNIIKNYVNPPAGRPPIFDVFSYYVAD
                     TFDRDFLIYYNQLKDDKTIDFNKINIKDQKQLGSALPLKQLKLGNFAPWNQLFEVSNS
                     TYILIVLFTLFLSAPLFSGDRAKKINPILLTTRYGRGQLTWAKLASTMTIGISTFILA
                     YALILTVFADYFGFSGWETSVQLNLYWTSPLANIMGFPVPMTISTAFVHIIVLQFVGL
                     LFIASLNFFISSITNSPLTSFAVSTLAFFAPSFLMAMFNKGVINKLLTIFSVSTTDTP
                     ALLLKLSRNGSPGFFFDSFSANEICLISLRMLIAVLLIAYTYRLMRRRSL"
     gene            complement(20319..21107)
                     /locus_tag="VBC52_001089"
     CDS             complement(20319..21107)
                     /locus_tag="VBC52_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6882685.1"
                     /translation="MSYQFTPGLYGLLGANGTGKTTLLNLMSHFTFADSGNIYWNGLE
                     QSEEFYRHIGFLPQHFRYYDQFTGIAFLNYIATLKGVDKKKAKQEIPRLLELVGLGDV
                     GKKKISSYSGGMKQRLGIAQVLINDPEILILDEPTVGLDPKERVKFRHILSQLSTNKI
                     IILSTHIVSDVEAVAKEIIVLKNGKFIEHGNTAQLLKTIEGKVWEITTEPDLSQIPNI
                     AIVNEKVISDSRVFRVVSDICPSDSAQLVVPTLEDFYIYHFRGE"
     gene            complement(21235..21408)
                     /locus_tag="VBC52_001090"
     CDS             complement(21235..21408)
                     /locus_tag="VBC52_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO6882686.1"
                     /translation="MKHYHMLKVALVIDGYTVRTGKHTYTSAWHLSEKTAAANARNRM
                     GLYPESVCRVYHV"
     gene            21697..21879
                     /locus_tag="VBC52_001091"
     CDS             21697..21879
                     /locus_tag="VBC52_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6882687.1"
                     /translation="MLTYKKALVRQVDTKDCGVTALASIAKFYGSKYSLNHLRELAKT
                     NRDGTTASGIIAGTTK"
     gene            complement(21950..>23175)
                     /locus_tag="VBC52_001092"
     CDS             complement(21950..>23175)
                     /locus_tag="VBC52_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO6882688.1"
                     /translation="IALRLGLVILFIAANTIINLSFLKYWLLPIYMLSLAFLLLRPIS
                     KTLVVFYGLFPIVLRNLCSRSLAFFVFPLFNIDFHTTNQGFWGYFVDVLSTLCVLLFL
                     RWMQYDFKALRSSEVSPTDRRILQFTNGCMVSYYLLMQFLTYFEYELAINTVIYRQFL
                     LVAYWIIFTGAIASLDRRLRNSLQERLAVQRELQLANMIDYSHHIEGLYKEVRSFRHD
                     YSNMLTTLKLGIDNEDISIVKSVYQSIFKESNTHFRHPKYNIDRLVNVDNPALKSLLA
                     AKLSQAIDNHIMVSIEVPDSIKPQGMELIDFITIVSVLWDNAIEASLETDDPKIAMSY
                     FTLKHKQIFIIENSTKEKAIDTSMIFNYEISSKGQNRGIGLYNVRGVINRYPNISIAT
                     SSQDFSFCQTFEIIL"
BASE COUNT         7556 a         4450 c         3772 g         7397 t
ORIGIN      
        1 gccatctttt ttagtaactt gaacttgact accattaata taattaaagc tttcaaaact
       61 tacactatat ttatttgata attggatgag ttgcttttca aaagattgtc tatccatacc
      121 atatttctta gcgttttgtg ccattcctag ttcatattca tctagctctg attcatcttc
      181 ttgtttttct ggttcttctt cgtctgaacc tttattaaca tcataaaaac catagttgtg
      241 agttactttc cctgaatctt caggttcttc atctggagca taatttttgg ctttattgtc
      301 tttatttgag cctttgttat gatcactgtc gtgtccccaa cctttttctt ttggtaattc
      361 attaggatgt tccatgtagt atttaattgt tgagaagagt tgttgtaatg tatagccttc
      421 tggtgctttc cataaaccac ccttgtcaaa ccatgccata ggaacattat gataatgatc
      481 atgatgagga acaatcaatt gatcatcttc aaatccaact gcatatgcca tgtgagcagc
      541 aattttatca agcggtacaa ttttttctgg ttttgcttgt ttgaatactt ctgcagcact
      601 taagttttta tcactagaat ttgatgttga tttattatca gttggtgcag gacttggttt
      661 atctgcttta ttagagtcct tttccttata aatttcagca tctttacccc atccttcaac
      721 tttaggacgt ttttctgggt gcatcatgta atatttagcc gtagctaaat agtcttctaa
      781 agaatatgtc ttatctgaat cagctaaaag atctttttct tcatcaaacc attttaattc
      841 cacataatgg taatgatctt tatgaggaat tacaaatgta ccattcttat agtctgtcgc
      901 ttgtgcaatt ccaaataata aatcttcagg tttaacaatt gcttttggtt caattgcctc
      961 atagatttct tgagctgttt tacctgtttt tggtttgtct tgatttgctt cagattttcc
     1021 tgaaagaaat tcttgagctg cttttaattc cgactctgat aaatctttct ttggtaccca
     1081 atgctcatgt gtcatatgtg gaatgacata acctgtacct gtatcttttt taatatcttt
     1141 ggcatcaaaa atgtaaccat cagatgttgt gtatttacca gctttttttg cctcttcaat
     1201 ttcttgagca ctgtagacaa tctggctatt tggtttgcct ttacgttggt ctttcggtgt
     1261 tactgcatgg tgttcttcct ctgtgtgagg ttccttatta ggttcatgag aaatatgctt
     1321 tggtttctct gtagaatctt ttttccctaa tgcatcaata tgatgtcctc tatgctctgc
     1381 caccatcctt gttgcttcta actctaatgg agacatatcc ttatagtgaa taaagtggaa
     1441 atgattaccg tgcggaatag aaacaccgat tgctgtgaaa gcattaactt gtgctgggtc
     1501 ataaaccaaa ccatcacttt ctacgtgacg agcagataac ggttgagcat ataaacgttt
     1561 caacattttg tctaaagcag aatctttgtt gattggtttg gttgtaggtg catggtgttt
     1621 tggtgccgta gcatgattac cagctggttt tgaccagtga tttgcgtgag tgactcttgg
     1681 aatagaaata ggggcattag catgtcttcc tgttgataca ttaccgcttg gtgcacttgc
     1741 atgaatgtat gaacttgaat ttgatggttt tgagctatta cctgatcggc cagtcttgcg
     1801 gttccaatat gcctgtgcgg ctgataactc tgatggagat aaatcagctt tcgggatata
     1861 atggaaatga tcgccatgtg gtactaaaaa ggcatctccc atgtcatcaa tgacatctgt
     1921 tggactaaaa acataaccat catctgtagt atagcgaccg ctagctttcg cttcacgtac
     1981 gctggctgat aaagcttgag tttttgcact actatgcttt cctgattctc ccttagaacc
     2041 acccttagac cattcttcgc gttgcttttg gatttgttcc tttgtacgaa catttgttcg
     2101 tttgctacct tctttaagat agaggtaata cttaccatta actttgataa tataaccatc
     2161 tttgacttca ttaatgacat cagctttatt aaagacatag ctagggtctt tcataatcaa
     2221 ttcttcacta atgatggcat catatggcac tttaccattg taataatggt aatggtctcc
     2281 atgagaagtt acataaccat catcagtaat tttaacaacg atttgttctg cagatatgcc
     2341 ttcttcttta ctgatttggt cgggcgtttt atttgcagct gttgcttgtg ctttgctatc
     2401 agatttatct gttttcacat aagaaacact attgtctgat ttttgggcat tataactacc
     2461 taattgataa gcacctacac cagtaccaaa tagagctact gataaagcac taataataac
     2521 tgttttttta cgattcatcc tgcgtccttt ctttatataa ctacctattg atttaacgat
     2581 ttgacaagta ccttaagatt agtttccaaa ttttctaagt aatctttatt gtttttggga
     2641 accgcttcta aaggacttaa acttgcaatt ttaactcgag tagctgaagc tactgcttga
     2701 gctaatttag gtgagactcc ttcttcaaca aaaatagtct taaccttata tgttttcaca
     2761 aactcctgaa tttcggctaa ttttttagca ctaggttctt gctcggttga gacacctgca
     2821 atacctaact gagtcaatcc gtatcgctta gctaagtatg agaatgctgt atgtgaagtc
     2881 acaaagtatt tagactttgc agctttaaat tttggcttat acttctctgc aatagccatt
     2941 gccttgtcac taaattgatc agcatttttt tgataaacct tagcgttttt aggatccttt
     3001 ttagctaatt gtgtagcgat gagttgtgct tcctcagata cttttacagg gtcattccaa
     3061 gtgtgagggt catacaaggt tgactcatct actccttttt ctgcctctac atcttctaag
     3121 ccatgaactt tatccaaagt catacctttt gaagcttcaa ttacagatac tttagagtga
     3181 tgcaaactag gttccaaacg tctcgcccaa gcttctagtg tgtgcgaatg ataaagaaat
     3241 agatcagcat cataaatggc agcaacatca cttgatgagg gttcaaaacc atgaatgcct
     3301 gactgtgatc gaatcatttt aatatcattc aaatcaccag aaactgcttt tgtaatggaa
     3361 tatactggat aaaagctagt cacgaccgac aagccatttt tttgctggct ctgtttagta
     3421 caagcacata agcaaattag ggtaattatt ccgagaacaa aaataaggct tttccgtatc
     3481 cttttcatta aacctccttt aaaataaaaa atacttaacc agttaacatt ttagagtatt
     3541 cttattttag tgtcaacgaa aaaccataaa tctaatattt ttctattttt aatattgtta
     3601 agtgcactta atttcatatc aacaaccttt attcatgcct ttccaagcta cagtaaacct
     3661 tttcataaac atgcatgggt taaggagtat aattagaacc acaataaaaa gagccaagca
     3721 ttttgcccaa ctctttggtt ttttatttat ttttagtatc taaaacgata gtcactggtc
     3781 catcattaat cagactgact tgcatatcag ctccaaattg acctgtttct accgaaacat
     3841 gatttgctaa ttcttgatta aagatatcat aaaactgatt cgctttaaca ggatcagctg
     3901 ccccagtaaa agctggacga tttccttttt tggtatctgc aaagagtgta aattgtgaga
     3961 tagataagac gcttccttta atgtcttgaa ttgaaaggtt cattttccca tcatcatcag
     4021 aaaaaatacg catgctagta attttacgaa ctgcataagc aatatcttct gttgtatctt
     4081 ctggagcaat cccaactaga agtactaaac ccttctcaat agatccgact acatcatctt
     4141 caatagaaac acttgcttga ctaacacgtt gaataatgat tttcattttg ctctcctaac
     4201 cattcgtacg tttaacagaa taaacatctg ggataatttt gattttatca accacagttg
     4261 tcaattgtgc taaatttgaa attccaaagc taacatgaat attagcaaat ttcatatctt
     4321 ttgttggttg tgcatttact gtcgatacta gctttgttgc atttgataag gtttgaagga
     4381 catcattaag gaggccacta cgattcaaac cataaatatc gatttcagcc atgtactctt
     4441 ttttagatcc atcatcgtcc cactcaacat caatcaaacg ttgttcgtag ttctcctgac
     4501 tctttaaatt ttgacaatct gaacgatgga tagccacgcc tctaccttta gttatatagc
     4561 cttcaatgag atctccagga acgggattac aacattttgc aatacgcatt aataaacctg
     4621 aagctccttg aatgataaca ccattttcac tcttaacttt gagaacatca cgcttatcgg
     4681 ttttgatttc gccaccgtta ataagttcgt ctgcttccgc tttagcctta gcacgttcct
     4741 tctcacggcg ttctttttcc gtcagtttat taaaaatact aatcggactc aaatcaccaa
     4801 aacctacggc tgcatacaaa gcttcctcac ttttaacact gacacgtgga aggatttctt
     4861 caatatgttt cttatctaaa tatttgttag gcacataacc ttgttcttgg aaataatcaa
     4921 ccagtaattc tctaccttta ttaatagaag tttctttatc ttggtttttg aagaactgac
     4981 gaattttatt gcgagcttta ttggtcttaa caatcttaat ccagtcacga cttggtccaa
     5041 atgaattagg gtttgtgata atctcaacaa catctcctgt ttttaactta gcagtcaatg
     5101 gaaccatacg tccatttact ttagcgccag tagctttttc tccaacttgc gtgtgaatag
     5161 cataagcaaa atcaattggt ccagattccc ttggcagttc ctgaacagca ccatttgggg
     5221 taaaaacata gatgcgttct gtaaagatat cttccttaac agagtcaaca aaatccttgg
     5281 cgtcttttga ttcatcttga agctcaacta attcttgaat ccatcccata ccaaccgatt
     5341 gctctgcttg attaacctta ctggtgattc cttttttata ggcccaatgg gctgcaacac
     5401 caaactcggc aacttgatgc atttctttag ttcgaatctg aatttcaatt ggccctttag
     5461 gtccataaac cgtcgtgtga atagattgat aaccattggc ctttggtgcc gcaatataat
     5521 ctttaaacct tcctggcata ggacgccata actcatggat ataccccacc atggcataaa
     5581 catcactagc agtttccata atacagcgta tcgcaattaa atcataaatc tgatcaaagc
     5641 gtttcttttt atcacgcatt tttctgtaaa tagaataaat gtgttttggt ctaccataaa
     5701 tatcaccata taaaccctgt tcctcagtat aggatctgat tttatcgaca attatatcaa
     5761 ccaactcttc acgttcacga cgtttttcac tcatcatatg agaaatttta taaaattccg
     5821 tctcattcaa gtaacgaaaa gataaatctt ctaattccca cttgatacga ctaatcccca
     5881 aacgatgcgc taaaggagcg tatatttcca tagtctcacg tgaaatacgc tcttgtttat
     5941 cttttctgag gtgtttaagg gttctcatat tatgtaagcg gtcagcgagc ttaaccaaaa
     6001 taacacggat gtccttagac atagccatta acattttacg gtgattttct gctaactgtt
     6061 cttcatgaga tttgtactcc actttaccca attttgttac gccgtcaata atatcgcgaa
     6121 catccttgcc aaaatcagtt tcaatctcat caagggtaat ttccgtatct tctacaacat
     6181 catgtaaaaa accacaagct accgtaacgg catcaaggtg taaatctgca agaatacctg
     6241 ctacttgaat tggatgaata atgtagggct ctcccgactt tcttgcttga taataatgag
     6301 ctgcggttgc gtaatttaag gcaaatttta caaaagcaac atccgtctca ctcatgtact
     6361 gggatgtgat tgcaacaacc tcttctcctg ttaaattaat ttctttgacc atagttatcc
     6421 tctttaatca tagtgataga ttgtacctat tttatcactt tttaacctaa aaataaaaga
     6481 agttagaggc tcaaaaagaa ttaaatacca agtaaatgtg tctcaccaag ttatccttta
     6541 taaataaacg attgtttttg gtgtaatcac caaaaataat tcgtaaataa gattgattaa
     6601 acgttttcaa taagctataa tagtttttag attaggataa tatacatgta atgttttaat
     6661 ggaggagatt tatgtcaaaa cattattttt caaaaagcgt attcgcttta actgtcttaa
     6721 cagctacagc aacaagtggt ttagctgttc aagcagaaga cattgttaca acaccaagtt
     6781 caaccgctac aaaggttgaa tcaactaccc ccactagcac aattgcagag gaaaagtcaa
     6841 acgttacttc tactccaact gcaataactg atgcttcaac agccacaagc acaaatacca
     6901 ctgccaataa tcaaaatcca cagcctgttg ccacagaaac cacaactggt gatctcaaac
     6961 ctattgaagg tgaaaaagtt gaccttcgca tcatgtcaac cacagaccta cacaccaatt
     7021 tagttaacta tgattattat caagataaag aatctcaaaa aatagggtta gcaaaaactg
     7081 ctgtccttat cgaagaagca aaaaaagaaa atcctaatac cgtcttagtt gataacggag
     7141 atgtcattca agggacacct ttaggaactt ataaagcaat cgttaaacct gtcgcagaaa
     7201 atgaagaaca tcctatgtac caagccatga atgctcttgg ttatgatgca tcaacactag
     7261 gtaatcatga gtttaattac ggtttagatt acctcaaaaa aatcattgct acagccaacc
     7321 ttccaatcct caacgcaaat gttttggatt tcaaaacaca tcaaccagtt tttaaaactt
     7381 atgacatcat tactaaaacc ttcaaagatt caacaggaag agctgttgca ctaaatattg
     7441 gaattacagg aattgtacct cctcaaatct taaactggga caaggctaac ttagagggaa
     7501 aggttattgt caaagattca gttaaagcaa tcgaagaaat cgttcctact atgcgtgcta
     7561 aaggagcaga tgtcattctc gttttatcac actctggcat tggagatgat cgatatgaag
     7621 aaggtgaaga aaacgttggc tatcaaattg ccagcatcaa gggagtggat gccgttgtta
     7681 cgggacactc acacgctgaa tttccatcag gtaacggtac tggcttctat gaaaaataca
     7741 ctggagttga tggtatcaat ggaaaaataa atggaacacc cgttacaatg gcaggcaagt
     7801 acggggatca ccttggtatt attgatttag gacttagcta tactaatgga aaatggcaag
     7861 tctccgaaag cagtgctaaa atccgtaaaa ttgatatgaa ctcaacaact gctgacgagc
     7921 gtatcattgc attggctaag gaagcacacg atggcacaat caactatgtt cgccaacaag
     7981 taggtacaac aactgcgcca attacaagtt actttgcact agttaaagat gatccttcag
     8041 ttcaaattgt taacaacgct caacgttggt acgttgcaaa tgaattgaaa ggaacacctg
     8101 aggcaaattt accacttcta tctgctgcag caccatttaa agctggaaca cgtggcgatg
     8161 ctaccgcata tactgatatt ccagctggac ctgttgcaat taaaaacgta gctgatcttt
     8221 atctttatga taatgttact gcacttttaa aagtgaccgg agcagactta cgtgaatggt
     8281 tagaaatgtc cgctggtcaa tttaaccaaa ttgaccctaa caataaagca ccacaaaata
     8341 ttatcaatac cgaatatcgt acctataatt ttgacgttat tgatggcttg acttataaat
     8401 ttgatattac acaacctaat aaatacaata aagatggaaa agttgttaat agtcaagcta
     8461 gtcgagtacg cgatttaatg tacaatggta agcccgttgc agacaaacaa gagtttatga
     8521 ttgtgaccaa taactaccgt gctagtggta ccttccctgg ggctaaaaat gccacaatga
     8581 accgtttgct gaacttagaa aatcgccaaa ctattatcaa ctatattatt tcagagaaaa
     8641 ccatcaatcc tacagctgat aataactggg gcttcacaga gtctattaaa ggccttgatt
     8701 tacgattcca aacagccgat aaagctaaaa atcttgttac taatagcaag gatattcagt
     8761 atatcgctag ttctaccaaa gatgaaggat ttggtgatta tcgatttgtc tatactgaac
     8821 aagaaaaagt agaccttact gagccatcac atcaagaaaa gttaagccaa atagaaaaac
     8881 aaaaaattca ccacatggtt gcaactgctt atacaaaagc aaaagcttct ccaaatatta
     8941 caggcactgt ctatacacaa cagaaagaaa aaacattacc taaaactgga caagaaaaac
     9001 aatcttatct ggctttcttt ggtgtcgcaa tacttggact cgtgggaagt ttacttaaaa
     9061 agaaacaaat atagtcatca ctaaaaactg aggccaatca acctcagttt ttagtctcaa
     9121 caatagattt tctctctaaa tagtcagata ttgagatacc actctagctt gtttatctga
     9181 catgattttg ctaaactgat aacaagaaat ttagatttaa ggagtactaa atgcctaaat
     9241 taaccctagt ctatatcagt cttagcggca atactcaaag ctttgtcaaa cgtcttagtg
     9301 aacaactgtc aacttggcat aactgtcagg tttcgactat taatatcaag gaacaaaatc
     9361 atcaaacctt tcctgttgac caacctttcg tagcactatt acccacttac ctcgagggag
     9421 gaaatggcat tgacaatggc gatcaagaaa ttcttaccat tccacttggt gattttatag
     9481 ctgcacacaa taatgtcaaa aattgtatcg gaattgttgg ctctggaaat agaaatttta
     9541 acaatcagta ctgtttaact gcaaaacaat actctgaacg gtttggattt cctatgttag
     9601 gagactttga attgcgagga acttctagcg acgttgaacg tatcgcaaat attatcgttg
     9661 aaacattatc gaactttaaa gcctaaagga aatagtatgg aaacaacatt aaactacatt
     9721 aaaaccctga catcaatccc ttcaccaaca gggtttaccc aaacaatcat gacctatatc
     9781 atcaaagaat tggaatcgtt tggctactca ccaattcgca caaacaaggg aggcgtcatg
     9841 gtttctctaa aaggaaaaaa tgatactaaa catcgcatga taactgctca ccttgataca
     9901 cttggtgcta tggttagggc catcaaacca gatggtcgat taaaaattga ccttattggc
     9961 ggatacacat acaatgccat tgaaggagaa aactgtacta tacacctctc aaaaaatggt
    10021 aaagaaattt ctggaactgc tcttattcat caaactagtg tccatgttta caaagacgct
    10081 ggaactgctg aacgtaatca aacaaatatg gaaattcgtt tagatgagaa agtaacaact
    10141 gctgacgaaa cacgtgcttt aggtatccag gtcggcgatt ttatttcatt tgatccgcgt
    10201 acaatcgtaa cagacagcgg ctttattaaa tcacgttacc tagatgacaa ggtatccgct
    10261 ggtatcctaa tggaacttct ttctgtttac aagaaagaag acattcaact tccttatact
    10321 actcatttct actttagtgc ctttgaagag ctaggacatg gagcaaattc aagcatccca
    10381 aatgaaactg tagaatatct agcagttgat atgggagcta tgggagacga tcaagaaact
    10441 gacgaatata ctgtctctat ctgtgttaaa gatgcttctg gtccttatca ttatgaatta
    10501 cgtcaacatc ttgtttctct agctgaaaac aataatattc cttataaact tgatatttat
    10561 ccttattatg gtagtgacgc ctccgctgcc atgcgtgctg gtgcggaagt taaacacgcg
    10621 ctacttggtg caggtattga atctagtcat tcttatgaac gtacccatat cgattctatt
    10681 caagcaactg aactcttagt ggatgcctat ctcaaaagca atatggtgaa ctaatggtag
    10741 ccacgctcat tattatcagg ggaaattcag ctagcggaaa gtctacaata gctaagcaac
    10801 ttcaagccga attaggggaa gatacccttc tcttatctca ggattacctt cgtagagaga
    10861 tgttaggaac taaggatggt gaaaatacta ctaccattcc ccttttgatc aatttactaa
    10921 attatggtta ccataactgt tcctacatca tccttgaagg catacttcgt tctgattggt
    10981 atacgcctgt ttggaaacat attttaaaac ataaccccaa caatacttat gcttattact
    11041 atgatttaag ctttcaagaa actgttaaaa gacattcaac tcgacttaaa tctttagaat
    11101 ttggagaaga ttctcttgca agatggtggc ttgagaaaga cttcttaaaa gagatacctg
    11161 aaaaaatatt aacaaaatct atgagtttag aggatgtcaa aaaacttata atgaccgacg
    11221 ttttacctta acattattgt acgcactata aacaataatg acttcttatc attggagtat
    11281 attgcccatg gatacagtga ttgacctctt atttattatc gttcccatta tagccggatt
    11341 tctggcatgt attagtcttt atcgttattg gaaaaaagat ttcgaaagcg acgaagacga
    11401 cgattaaact cctcggaatc aatgattccg aggagttttt ctatattctc atttaatcag
    11461 catttctacg aatacgatta taggttttaa ggatttggcc cttatgctta gtgacatagc
    11521 gataatcttc tttaagagta gcaatatcct ttaaagcttt catgccatta ctcgtttggg
    11581 catctttacg aataggtcgg ttactcgttg actgcccaaa ggcattttga acatctaaag
    11641 aaagcataaa attaataaat aactttgctt ctttcataga aggagcattc tttataattg
    11701 caaccgaaga tgggacaaaa actgtccctt ctgtcggata cacaatagaa acattggcac
    11761 cacttttttg caaattgaca ctagggtctt cgtaagtcag ccccacaatc attttgcctt
    11821 ctgcaactga ttgataaact tctgaagagc tagaagattt gatagcatta atattatgtt
    11881 gtagcttttt aacatagttc cacgcttttg gattggtgta accacccttt gccaagagta
    11941 tattggtgag ttgtgagaaa gcactagagg aagtattcgg atctgcaaag gcaattttac
    12001 cttttaagga aggctgtaat aaatcttcat aactcttgat ggtaagtccc ttagctaatt
    12061 cgttatttac aatcaagaca ctcccattta tagtataagg tgtcgccgta tcactcggat
    12121 ggatatagtc tggaataaca gtatgaacat tctttgatac gtaagactca aacaatgcct
    12181 tatgactttc aaattgcgta taatttcctc caaagaaaac atccgccttc aactgcttac
    12241 cctccttact taatctatct attagttgcc ctgtcccacc ttgaataagc ttaactttta
    12301 taccgtattt ttcctcaaaa gctggaatcg ttcctgttaa aatggcttga ctatttggac
    12361 ttagaataac taattctttt ggtggaagta gtttagaagg ctggctaata ctgtatgtaa
    12421 aaatacttat aaaaataatg ggaacaacca gtagtatata aataagcctt ttcgactgtt
    12481 tttctttcat ctttcaccta cctaatccat ctgtttaaaa atatgttctt ccttaaacgc
    12541 ttttactgtc tgacccaagt actttttaaa aaccctatca aaataacgat aatcataaat
    12601 accaacagct tctgcaattt ggtaaaccat taggtctggt tctctaaaca tcaactgaat
    12661 ggcttgatta atacgatatt gatttatata atcactcaaa gttatatgaa ggtgcttttt
    12721 agtgactgtg taaagataac tttcactgta acctaaatcg tgagctaact ggctaatcac
    12781 aattttccca tgaaaatggc tatgaatcca tgctagcata tctttaataa cttcaggaac
    12841 ttggtcatta gcttgtggta gaggtaaata caattctgta tgctcttgta ggtgcttctc
    12901 cttatgttcc tcaattctag ccataattgt ttttaaacaa tcaatcaaag cttgcttttc
    12961 tagtggtttt gttaaaaatt caagaacgcc atagtgaata gctgattgcg catttggaaa
    13021 atcagcatag ccagataaaa taattttggc ataagattgt cctttggttg cctcaaacat
    13081 atcgaatgct gtcataattg gcatattaat atcacttaaa acaatgtctg gctgctcttc
    13141 ctgaattaat tgagctcctt ctttaccatc ttttgcttca ccaaccacta aaatattcaa
    13201 tgattgataa tcaatagcat agcgtaacca cttacgaata aggtgctcat cctcaacgat
    13261 aagtaatcta tacataactc actcctttac tatatagcta actcgaaaag catcatctgt
    13321 cttcgtaaac tctaacaata ctgttgaaaa ttgatattgt aatcgtctat aagaattaat
    13381 aagcccatga tgcgagtgag ttgatcttaa cattgtacgt atggcttttt gcctagccaa
    13441 actaatacct gatccattat ttgaaacagt aaaccaaatt ccatcactat cttgccaaat
    13501 atctatgttt attgctacat catgacgttc ttttaagcca tatttaatag cattctcaat
    13561 tagcggtaac aagaaaagtt ttggtacacg catgtgttca agttcagggg atactgaaat
    13621 agtgtaagtt aattcttcaa atctcacttg attaatcaaa aggtaggatt caatgattgc
    13681 taaatcatcc ttaagcacag ctgcttctgt gctaccacta aggctatagc gcaataattt
    13741 tgttagttgt ataacgattc tttctgttaa ttgtgaatca tagtgactag ttattaaaac
    13801 tgtctcaagg gtattataaa ggaagtgagg attaaactga gcttctaaca tcctcttttc
    13861 aaacagcaac ttttgtgttt ccaaatcgag agttttctca tgtaaatctt ttaaccgtga
    13921 aaccatctga ttgatttgaa cagatagata ttgaaattca tcttgactat caagctcaat
    13981 actagatttt tcttggcggg aaatggcatc catatctagc accatttgat ttatggcgct
    14041 agaatttttt actgcgatgc gatcggctag aactctagat tttcgtcgta aaattacaaa
    14101 aataataact gatgatacta gagaaaaaag gacgaccaga gttactggta tcaggggacg
    14161 atacatgtaa agtaggatat tgtcttgcag agctactttc ctaacaacaa aagctcgatg
    14221 atcgtgaaaa ctaaaatatc tagttaagaa ttgactatct accttatcta aactagagga
    14281 gataaaatca cgattagtaa aagtaaagct attatttagc tgatcggcaa taatcaagtc
    14341 tgagttaata gccttcgttg gaagtaaaaa gtctttccca ttcatcaata agaaggcata
    14401 acctgaaact tttttattaa ctataattgg cttaatgagt gcaaggtaat gccctccctc
    14461 actatctaca ataattttta gtagcctttg atttatttta gccttatcta aaacttcact
    14521 aatgtaaata gacttactaa aaaaattacc taaatgtgaa ttactagcaa acaaaagttc
    14581 ttgattaggt gagaatacta aaagttctgt ttgactttgt aaatgattcc ttaaatgata
    14641 atattgctta ctcaaatcag cacttcctaa gtgtcctgag acaaatggtt tcaaactatt
    14701 tttacctaag tcatctaata aggtatcatg tttttgaatc aattgcttat aactatagga
    14761 aactgaatta acatctttat aaaggcctat ctgctgaggc gctaaagaaa aaaggacgaa
    14821 aagtgcaatc aataacaagg ataaaattaa agactggcgg gaaaaatgtt tagagatatc
    14881 atcttgtagt cgtttcttaa agcgttcttc cattctatat cctctcattt ccatctcctt
    14941 ttattatagc aaatagctat ccaataaaga tagtcaactc ctttcataca ataactttca
    15001 aaagaaaaaa agataagcta aacaagtatc gtcttgttcc acttaccttt aatgctagga
    15061 gattattagc aaatacctaa gagaccaagt acaataccta acaccataat gccaaacatg
    15121 atccagttga tattgacttt tttacgtaga agccagaagg ctaatatcgt taccaacagt
    15181 ggcactacgc caacaaataa ttggtcaagg taactttgta tggaagctgc ttccttagct
    15241 cctttagctg ctaccgttaa gattgtcttg aatttaacat ttgaagcagt cattgagcct
    15301 accatcatta aaccaagcat acttgaagtc ttagtaacaa ttttaatacc acctgattca
    15361 tacaattttt tgataaaccc tgcacctaca gagtaacctc catagagact ataataatga
    15421 atcagaaaag ctggaatgtt atacaagagt agaaagacaa cagctcccat ggctgagcct
    15481 gtacttgcca aggagatacc gatacctgcc gcaataactc ttagaattcc ccaaaagaat
    15541 gaatcaccaa ctccagatat aggtcccatc aaagaagcct taactgcggt gatggcacta
    15601 gcatcgaaat caggatcttc actgtttttc ttttccatag atgcaacaag acccataata
    15661 aaattattaa tgtgcatggt ggcattaaac catgttgtat gacggaccaa tgcctctgct
    15721 ttatccttat cagtcttata aaaacgattg atgactggta gtaaagtata aataacccct
    15781 actgcgtggt attgtgttgc cccagtacga ctagcattca tcgtccatga gcgccagaag
    15841 actgagcgaa gcattttacg gtcttcttta gtcaaattat cctgtgattt catgcgaaga
    15901 agtcctcctc ttctgattct ttagagtcaa aggttgtctg tttactgatc gcacctctag
    15961 taattgcgtc taattctata tcacgttgtg aactaatgac gcaaataact gctcctaggg
    16021 cagcaactgc aaccgctggt aattttaaat aagctgttaa aacaaaacca agcaagtaaa
    16081 aaacagctaa tttatttgtc cataataatt tcatcagcat agcaaaacca acagctggaa
    16141 ggaggcctcc cgccgcactt aagccattca tcaggttttg aggaatatga tggacaaaac
    16201 tattaacagg cccactacct actaaaatac caatgaaaga aatagaagcg ataatgagat
    16261 agtaaataat ccatgtacca tagtgaagca tcacaagttt accttgatgg tttgcggctg
    16321 cagctttatc caccatcggt gcaaagatat tcatgaagac gtttttcaaa aacataacaa
    16381 caaatgctgc caacattcca attggaacag ctagagtcat tgctgctttt tgatcaatgt
    16441 ttgaaataat ggtaaaagtt gttgccatcg ctgttgctgt tactggctct gctgcaataa
    16501 cacccccaat attaacgttt cccaaaaata gcgcttctag ggaggctccc attaagatac
    16561 caaccttaat atcacctagt agcattcctg ttacaagacc aactacaagt ggtctctcca
    16621 tcatactttg acctgttaag taatttcccc caaaacaaat caatacagct aaagctgcca
    16681 tcgtcgctgg cattatcata tgtcatacct cactttattt gactagttga ctcaagttaa
    16741 ctttagtatt tgaaggcaat acttggttaa agacgtctaa atctggtaag ctggctaatt
    16801 cttttgcggc ttccaactct tctggattta ataagagact tgatgttagt ttgacttttg
    16861 tagctggatc agacttatca aagcgtccta cattagcgac gtttactgct tctaaatcag
    16921 ttatattttt agcaattgta caggcatctt taacagaatt gacaatcaca aaaatacgct
    16981 tgtcagttgc gcgtggatct ttaaatagag ctattgattc ttcaactgaa cggattaata
    17041 atttcatgcc attaggcaca gccattttaa gagtcatctg tgtgatttca ttttttgcag
    17101 cttcatcatt agccaccact aaaagtggtg catttaattc tttagtccat acaactgcca
    17161 cttgcccatg aatcaaacgg tcgtcaacac gaatttgagt aatcattttt ttcctcctaa
    17221 ttattaatcg aaaaagtcat cttctgaact cgctttaaag ctaagatcta ccagctgcac
    17281 ttgagattca ttgataacgg ttacaatttc ttctttagtt aattcctctt caggtttcaa
    17341 aaatagtaat gacaacaagg tcgctaaatt gctgttcgtt attaaaaaaa cattattttt
    17401 cccactattc attacagcag tcgccatttt ctgattaacg ctaccaccta acaaatctgt
    17461 aaaaataagg ccctgttcat ctggtgcaat ctcatcaata aattctacaa tagacttggt
    17521 atagtcacta tcatcaatgt aagcatctat tgtctgaatc tcttgtccct cgcctgtcag
    17581 aatatcaata gaactctgca acccactcgc aagttttccg tgacttccaa ttaggaattt
    17641 ccgcttcatc tttctcctcc tatttattac attaccctaa attagttctg aaagttttat
    17701 caatactttg aataagataa cctttactat cattctaaca aatgaaagcg gtttaaaaat
    17761 ggactattct actctatgtg tggactattc tatataatta atttttaaaa acagaacaat
    17821 aaaaaggtca tgtatcctaa tagtttacac aacctttaca tcactataac tttaaacttt
    17881 ctcttatatg aatcactatt tcgaaagaga taatggaata attttttgat agggtatctt
    17941 attaacaacc tttattatga gtagagcaca agcatgtgtt aatgccaagg tcaccacaaa
    18001 aatcactttt ccttgatggt gtgcaactaa ggaagttgat tttaaacagt aagacactat
    18061 gccaacaaaa agagggtgaa ggaagaaata atagacactc aactgcttta aaggacttaa
    18121 acgatagtcc ttaaacagtg atgtacgtat actccagtta aataagaata ctgcacataa
    18181 aggagctaat aagaagaaat ttttatccaa cccttgatta aaataaataa gaacattctc
    18241 taaagctaga agtaaaagga agagaatata cttaagacca cgcgatttgg taaataggtc
    18301 agtattaaaa taatcataga gtaaatatcc tgctagaaga taaactggag tgtaaaataa
    18361 tccgtttcgt gttgtctgaa aaattgacat ataagtggac atcaggggat aaaattttgt
    18421 tgactgtaca aagacagaat aggtctctac tgctccaata caatagagta caacaactgt
    18481 gataaatgtc cccctagttc ctacatactt caataatcct tgaataatga tccaacctag
    18541 aataacagca gggatgtacc aaagggtatg taacatacct aaatacaaca atgccacaat
    18601 aaaacccggc agcaaataga tcttcgctat atttagagat tcaaaaaaga aataaccata
    18661 aggcaaataa atgatagtcc acatgctata ctttttaatc agtttacgaa agtaggagtg
    18721 attacacaaa ccttgctgta tacgacctct caaaaagaaa gcagtacaaa tcaaaaaata
    18781 aggaacagcc atccgaccta ggaaactttt aaaggtaaaa tgaatgacat cctgactaaa
    18841 taaccttcca ctatgaacta aaatgaccag tatggaaaaa aggtattgta gcaagctaat
    18901 cattgatgta ttatgctgct tattatccac gcactgactt ctcttcatcc ttaacattac
    18961 tttctataag ttatcattac tatgatacca atctcttcag aagaatatct caattatccc
    19021 aaaaagtcat tgaaacgcct taaatggtat ctgataatta ttgttaatac cttattaacc
    19081 atattaggaa ataaaaaagc tgatttcgca aaagaaaatc agcttagaat aatttcttgt
    19141 tcagataact agtgggagct ataaacttct tcgtcgcatt aaacgatagg tataagctat
    19201 caataacact gcaattaaca tacgcagact gatcaaacag atttcattag cagaaaaact
    19261 atcaaagaaa aagccaggac taccattacg tgataatttt aaaagtaaag ctggagtgtc
    19321 tgtcgttgat actgagaaaa tagttaataa cttgtttatc acacctttat taaacatagc
    19381 cattaaaaag ctaggagcaa aaaaggcaag tgttgaaaca gcaaaagatg ttaatggcga
    19441 atttgtgata cttgatataa agaaattaag actggctata aaaaggagtc caacaaattg
    19501 taagacaata atatgaacaa aagcagttga tattgtcata ggtacaggaa atcccataat
    19561 attagctaaa ggtgatgtcc agtataaatt cagttgaaca cttgtctccc aaccactaaa
    19621 accaaaataa tctgcaaata ccgtcagaat gagggcatat gctaggataa atgtggaaat
    19681 accgatggtc attgtactag ctaacttagc ccaggtcaac tgaccacgtc cataccgagt
    19741 cgtcagtagt ataggattaa tcttcttagc cctatctcct gaaaaaagag gagctgataa
    19801 gaatagagta aataagacaa ttaaaatata agtactatta cttacttcga aaagttgatt
    19861 ccaaggagca aagttaccta actttagttg ttttagtggt agcgcagaac ccaactgttt
    19921 ttgatcttta atatttattt tattaaaatc aattgtttta tcatctttca attggttata
    19981 gtaaattaaa aaatcacgat caaaagtgtc tgccacataa taactaaaaa catcaaatat
    20041 aggggggcgt cctgctggcg gattaacata gtttttaata atatttgtta ctcgttcttc
    20101 agataaatcc cctgaatatt tttcagctat tctacgatta atggcaaccg cttctcgacc
    20161 atgtatttta tctgcagaat agccactaat ttgcccaaca aaaaaatgct ggtaaaatag
    20221 accaaaaata gcaagaagga agataataaa agcccccaat gctgccttac gttgtagcag
    20281 tttttttaat tcaaaaggaa aaattgatat catcttactt actcccctct aaaatgatag
    20341 atgtagaaat cttctagagt cggaacaacc aactgtgccg aatcactcgg acaaatatca
    20401 ctcactacac gaaatacccg actatctgaa atgacttttt cattaacaat agcaatattg
    20461 ggtatctgag ataaatctgg ttcagtcgtt atttcccaca cttttccctc aatagttttc
    20521 aacagctgtg cggtgttgcc atgttcgata aatttaccat tttttaatac tataatttct
    20581 ttggcaacag cttccacatc cgacacaata tgcgttgata aaatgataat tttattggtg
    20641 gataactgac tcaaaatatg gcgaaattta acccgttcct taggatcaag tccaacagtt
    20701 ggttcatcca gaatgagtat ttcaggatca ttgataagta cttgagcaat gcctaaacgt
    20761 tgcttcatac caccggagta tgatgaaatc ttctttttac ctacatcgcc taaaccaaca
    20821 agctctagta atctaggaat ttcttgttta gctttctttt tatcaacacc ttttaaggta
    20881 gcgatataat ttaagaaagc aatgccagta aattgatcat aatagcgaaa atgttgcggt
    20941 aaaaaaccaa tgtgacgata aaattcttcc gactgctcca gtccattcca ataaatattc
    21001 ccactatctg cgaatgtaaa gtggctcatc aaatttaata aggttgtttt gccagtacca
    21061 tttgctccta agagtccata caaaccagga gtaaactggt aggaaatctg gttaagtact
    21121 atttttttcc gtagctttta gtgatattat ctagctgtaa taacatagta ttgtctctcc
    21181 ctatacaaac ttaagcggca aggaagattc cctatcgctt aaataaaaaa tcaattatac
    21241 atgatagaca cgacaaacgc tttcagggta aaggcccata cgatttcttg catttgctgc
    21301 agcagtcttt tcagaaagat gccatgcact tgtataagta tgtttaccgg ttcttactgt
    21361 atatccatct atgaccaagg ccaccttcaa catgtgataa tgcttcatta tccataactt
    21421 ctaattgttc aattgatttt acatttattt tgaatatctc ctgtataaaa tttttgtctg
    21481 ttgcaacttg acacatttaa tatagcattc aaataaaaat agaagcgttt attccctgaa
    21541 atgtcgttga atcgcctcaa aacagaaaag tactatctac ctttatattc ttatagaaac
    21601 cattggacgc atttcaatag ctgtttaagc tatttttaat aagtatatat ttttatggca
    21661 aaatattaat acgataacga agaaaggaat tacacaatgt tgacgtacaa aaaagcattg
    21721 gtgcgtcagg ttgatactaa ggattgtgga gtaactgcac ttgcttcaat cgcaaagttc
    21781 tatggttcaa aatattcatt aaaccactta cgagaactgg ccaaaactaa tcgagatgga
    21841 actacagctt caggaattat cgccggaaca actaaataat atcactggtg gtggttggat
    21901 tgatggtatt aagaaaataa taaacttaga taaattgaac tttttaaagc tataatatga
    21961 tttcgaaagt ttggcagaaa ctaaaatctt ggctacttgt tgcaatagaa atatttggat
    22021 aacgattgat aactcccctg acattgtaaa gacctattcc tctattctgt cccttactac
    22081 ttatctcata attgaatatc attgacgtat ctattgcctt ttccttagtt gaattttcta
    22141 tgataaaaat ttgtttatgt tttaatgtaa aataagacat ggctatctta ggatcgtctg
    22201 tctctaaaga agcctcaata gcgttatccc ataaaacaga tacaatggta ataaaatcaa
    22261 tcagttccat cccttgaggt ttaatactgt ctggtacttc tatagatacc ataatatgat
    22321 tgtctatcgc ttgtgaaagc ttggctgcta aaagactttt taaggctggg ttatctacat
    22381 taaccaaccg gtctatatta tacttaggat gtcggaaatg tgtattagat tctttaaaga
    22441 tagactgata aactgatttg acaatagata tatcttcatt atcaatccct agtttcaagg
    22501 tcgttaacat gttactataa tcatgtcgga agctcctaac ttctttatac aacccctcaa
    22561 tatggtggct ataatctatc atattagcca attggagttc tctttgaaca gctaacctct
    22621 cctgtaaaga atttcgaagt ctccggtcta aactggctat tgctcccgta aatataatcc
    22681 aataagcaac aagcaaaaat tgtcggtaaa taacagtatt gatggctaat tcatattcga
    22741 agtaagttaa aaactgcatc aataagtaat aactcaccat gcacccattc gtaaattgga
    22801 gaattctcct atctgttggc gaaacttcac tacttcgcaa tgctttaaaa tcgtattgca
    22861 tccatcttaa aaataagaga acacaaagag tagacaagac atcaacaaaa tatccccaaa
    22921 acccctggtt ggttgtatga aaatcaatat taaacaaagg aaagacaaaa aaagctaaag
    22981 aacgtgaaca aagatttctg agaacaattg gaaacaagcc ataaaagact actaaagttt
    23041 tagatatagg tcttagcaac aagaaagcca gacttaacat atagatgggg agaagccagt
    23101 actttagaaa tgaaagatta ataatagtat tagctgctat aaataaaatt acaagtccta
    23161 atctcagagc aatac
//
LOCUS       DATRNK010000028        22299 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.028, whole genome shotgun sequence.
ACCESSION   DATRNK010000028 DATRNK010000000
VERSION     DATRNK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22299
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.028"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            6..245
                     /locus_tag="VBC52_001093"
     CDS             6..245
                     /locus_tag="VBC52_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882689.1"
                     /translation="MKEKLLVPALNCLLGLLFLGDHWFLFRLQNGELFAIVTLILLCT
                     TSFLINKNHPKNLTLIAMVITFLAVLSFLSTFLLA"
     gene            430..1497
                     /gene="pepA"
                     /locus_tag="VBC52_001094"
     CDS             430..1497
                     /gene="pepA"
                     /locus_tag="VBC52_001094"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEO6882690.1"
                     /translation="MSDLFNKIKTVTELDGIAGYEHNIRNFLRQEITPLVDQVETDGL
                     GGIFGVKNTHETNAPKVMVAAHMDEVGFMVSHIQPDGTFRVLEVGGWNPLVVSSQRFT
                     LYTRSGDAIPVISGSVPPHFLRGQSGGTTLPKISDIVFDGGFTDKNEAESFGIAPGDI
                     IVPKSETILTANQKHIMSKAWDNRYGVLMVTELLKSLKDQSLSNTLIAGANVQEEVGL
                     RGAHVSTTKFNPDIFLAVDCSPAGDIYGEQGKIGEGTLIRFYDPGHIMLKDMRDFLLT
                     TAEEAGIKYQYYAANGGTDAGAAHLKNSGIPSTTIGVCARYIHSHQTLYAMDDFLQAQ
                     AYLQAIVNKLDRSTVDIIKGY"
     gene            1567..2337
                     /locus_tag="VBC52_001095"
     CDS             1567..2337
                     /locus_tag="VBC52_001095"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEO6882691.1"
                     /translation="MKIGIIGVGKMASAIIQGLKQTQHDIIISGSCLERSKEIAERLD
                     VTYAESHQSLINQADIIMLGIKPQLFEKVLLPLDITKPIISMAAGISLARLSQLTRSD
                     LPLIRIMPNINAQILQSCTAICYNNHVSDELRQLAKEITDSFGSSFDIAETNFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKYGFPKEQALSIVGQTVLASSQNLLQGQNSTSDLIDN
                     ICSPGGTTIAGLLDLEKNGLTHSVISAIDATIEKAKKL"
     gene            complement(2358..3023)
                     /locus_tag="VBC52_001096"
     CDS             complement(2358..3023)
                     /locus_tag="VBC52_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6882692.1"
                     /translation="MSFIIRKGKILALLIAFLVINQLVPILAVWLLKNHYQTPFTSIL
                     LIGLELLIIALFLYYAKVKQIIRWKALLTRKALVTILLGWLSLRVPQIIGYLIMTMQG
                     VKDTANQTVIMELTKQLPLALMLIFAIIGAPIMEEIIFRYIIPKELFAKHQKWGFVIG
                     TLAFALIHSPSDIGSFIIYAGMGAILSFVYYKTEHLEYSIMIHFINNALAYSVLISTM
                     MNN"
     gene            complement(3092..3547)
                     /locus_tag="VBC52_001097"
     CDS             complement(3092..3547)
                     /locus_tag="VBC52_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882693.1"
                     /translation="MLIKGLLSPNSKVSPKDYKVYMRVLSFVFSVCSLIMIVSSFLHT
                     FESNFTKGFSFGSGIALFAGSFYFLVITFSEKRFQHYYTKVFDERNQFIANMSCRLLF
                     LLSVLVLVVLCLINVWFGITFSYAKFLLLILSFILYGYLLIYFILQKLL"
     gene            complement(3588..3725)
                     /locus_tag="VBC52_001098"
     CDS             complement(3588..3725)
                     /locus_tag="VBC52_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882694.1"
                     /translation="MSRMWTIDFCPPIVYQQYVFIITISISIAMSIFAIYRYASLKKT
                     I"
     gene            complement(3784..3990)
                     /locus_tag="VBC52_001099"
     CDS             complement(3784..3990)
                     /locus_tag="VBC52_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6882695.1"
                     /translation="MKNSLQKLRKSRKLSQAELAVALGVTRQTIISLEKEKYTVSLEL
                     AFKIARYFDKQIEEVFIYTESEGG"
     gene            complement(4142..5335)
                     /locus_tag="VBC52_001100"
     CDS             complement(4142..5335)
                     /locus_tag="VBC52_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEO6882696.1"
                     /translation="MSKTIAINAGSSSLKWQLYEMPEEKVVAKGIIERIGLKDSISTV
                     KFDDKKDEQILDIVDHTQAVKILLEDLTKHGIIKDFNEITGVGHRVVAGGEYFKESAL
                     VDDKVVEQVEELSALAPLHNPAAAAGIRAFREILPDITSVCVFDTAFHTTMQPHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHQYVAQEAAKQLGRPLEELKLITAHVGNGVSITANYH
                     GQSIDTSMGFTPLAGPMMGTRSGDIDPAIIPYLVANDPELEDAAAVVNMLNKQSGLLG
                     VSGTSSDMRDIEAGLQSKDPNAVLAYNVFIDRIKKFIGQYLAVLNGADAIIFTAGMGE
                     NAPLMRQDVIAGLSWFGIELDPEKNVFGYFGDITKPDSKVKVLVIPTDEELMIARDVE
                     RLKAK"
     gene            complement(5367..6341)
                     /locus_tag="VBC52_001101"
     CDS             complement(5367..6341)
                     /locus_tag="VBC52_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6882697.1"
                     /translation="MNFEKIETAYELILENIQTIENQLKTHIYDALIEQNSYYLGSSC
                     DLDMVVVNNQKLRQLDLSQEEWRRTFQFIFIKSAQTEQLQANHQFTPDSIGFILLFLL
                     EELTSQETVDVLEIGSGTGNLAQTLLNNSSKELNYMGIEVDDLLIDLSASIAEIIGSS
                     AQFIQEDAVRPQILKESDVIISDLPVGYYPNDGIAKRYAVSSSKEHTYAHHLLMEQSL
                     KYLKKDGIAIFLAPENLLTSPQSDLLKEWLKGYADVIAVLTLPETIFGSRQNAKSIFV
                     LKKQAEQKPETFVYPLTDLQNRENMANFIENFQKWSRENSHYSKNMIK"
     gene            complement(6456..6827)
                     /locus_tag="VBC52_001102"
     CDS             complement(6456..6827)
                     /locus_tag="VBC52_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGG"
                     /protein_id="HEO6882698.1"
                     /translation="MILKKKLKAGILLQAIVLAAVFTLVLQFYLARILATERQYHSQI
                     EASKAYLTAQLAYKTIEGDSISGKCYFTGGYASYLQEGNYLQVKVTLDKGGNYNHKFY
                     RKTDTLQVPLKSNDNKASHKH"
     gene            complement(6805..7266)
                     /locus_tag="VBC52_001103"
     CDS             complement(6805..7266)
                     /locus_tag="VBC52_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001880689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEO6882699.1"
                     /translation="MKIIRKFCTYQKLRVKAFTLLKCLVALVTITGALLVYQGLTKLL
                     AQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQNKLYLRKQDKIVTFGKSNKDDF
                     RKTGYDGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET"
     gene            complement(7220..7519)
                     /locus_tag="VBC52_001104"
     CDS             complement(7220..7519)
                     /locus_tag="VBC52_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type II secretory pathway, pseudopilin PulG"
                     /protein_id="HEO6882700.1"
                     /translation="MVIIVRKKIRAYILLESLVGLAILVTITRLVISEINRQQSHIAR
                     RQHEQEVLILARMAVQTDQKILEANGVNIRIEESKNKIVLFEDNKEILHVSKVKG"
     gene            complement(7491..7904)
                     /locus_tag="VBC52_001105"
     CDS             complement(7491..7904)
                     /locus_tag="VBC52_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEO6882701.1"
                     /translation="MVLRKFQVKAFTVLESLIVLSVVAFMTLVFSTSFNNIFRQVEET
                     IFFISFEHLYRDTQKLSAFGQKKQTLTISHNYLENTYERLYLPKTVKVVKSDTLAFDT
                     NGGNSSLAKIQFECYRKTVTYQLYIGSGNYRKKEN"
     gene            complement(7879..8208)
                     /locus_tag="VBC52_001106"
     CDS             complement(7879..8208)
                     /locus_tag="VBC52_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEO6882702.1"
                     /translation="MKNLLLKCKDKKVKAFTLLEMLVVLIIISVLLLLFVPNLSKQKE
                     SVTRTGNAAVVKVVESQAELFELQETGRKASLSTLKSGGYITEKQEKAYLDYYKDSSN
                     GSQKISS"
     gene            complement(8205..9296)
                     /locus_tag="VBC52_001107"
     CDS             complement(8205..9296)
                     /locus_tag="VBC52_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEO6882703.1"
                     /translation="MTLRQVILKNTHQTSGIDKWISWLKKDISVRNRHKSKKLSLKKQ
                     RKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDCYTDSMNKALLEGKDLSKMLGELGFS
                     DTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLSFLVLIMIGL
                     RNYLMPQLGENNFATRLITNVPNIFLLLLAVVLIFSLIFYIIQKRLSRIKVACFLTTI
                     PLVGSYVKLYLTAYYAREWGNLLSQGIELDQIVKVMQNQKSKLFREIGYDMEEGFLSG
                     KAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQP
                     VIFIFVALIIVMIYAAMLLPMYQNMEILS"
     gene            complement(9181..10113)
                     /gene="tadA"
                     /locus_tag="VBC52_001108"
                     /pseudo
     CDS             complement(9181..10113)
                     /gene="tadA"
                     /locus_tag="VBC52_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            complement(10286..10651)
                     /locus_tag="VBC52_001109"
     CDS             complement(10286..10651)
                     /locus_tag="VBC52_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEO6882704.1"
                     /translation="MYQVVKMFGDWEPWWFIEGWEEDITEIAEYDTLSEALLYFQEEW
                     DRGQEKWPYFQSKSSLLATFWSIKEKRWCEECDEYLQQYHSLMLLKEWQEIPKEESIE
                     RFEVFNKIAELPSACSLNL"
     gene            complement(10765..14415)
                     /gene="rpoC"
                     /locus_tag="VBC52_001110"
     CDS             complement(10765..14415)
                     /gene="rpoC"
                     /locus_tag="VBC52_001110"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEO6882705.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIICDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPMDTPLEPKSLLTEREYREKLQ
                     EYGYGSFVAKMGAEAIQDLLKRVDLDAEIAVLKEELKSATGQKRVKAVRRLDVLDAFK
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDLAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDHDEAVMAYQNGYVHLHTRVGIAVDSMPNKPWTEEQKHKIMVTTVGKI
                     LFNDIMPEDLPYLIEPNNANLTEKTPDKYFLEPGQDIQAVIDNLEINIPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     DINKAFRRGLMTEEDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLTITAITDGKEVTETLEERLIGRYTKKSIKHP
                     ETGEILVGADTLITEDMAAKVVKAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGEVVAIEEDSSTRTKKVFVKGQTGEGEYVVPFTARMKVEVGDEVARGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPLAVNEVEIIEGTPVDA
                     EVTEVSTPTTED"
     gene            complement(14532..18107)
                     /gene="rpoB"
                     /locus_tag="VBC52_001111"
     CDS             complement(14532..18107)
                     /gene="rpoB"
                     /locus_tag="VBC52_001111"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO6882706.1"
                     /translation="MAGHEVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDAG
                     LKEVFEDVLPISNFTDTMDLEFVGYELKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDAKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGDSELVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNLKTRLLNQTIAENLVDGETGEILAEAGTVMTRDVIDSIAEHIDGDLNKF
                     VYTPNDYAVVTEPVILQKFKVVAPTDPDRVVTIVGNSNPEDKVRALTPADILAEMSYF
                     LNLAEGIGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRSTGAVTNEIV
                     WLTADEEDEFTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFPSSIVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAKH
                     DGRVIFSDAEKVEVRREDGSLDVYHVQKFRRSNSGTAYNQRTLVKVGDLVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEDSLRDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGVVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWETVQEAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDVMHVDDLEKARVKQEAEEKQAEQVSEVVQED"
     gene            complement(18631..20928)
                     /locus_tag="VBC52_001112"
     CDS             complement(18631..20928)
                     /locus_tag="VBC52_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6882707.1"
                     /translation="MFKGNKKLNSSKLGDYTPLEFGSIFLRIVKLLSDFIYVIISLFV
                     MLGVGLAVGYLASQVDSVKVPSKNSLVTQVNTLTRVSRLTYSDKSQISEIDTDLQRTP
                     VAKDAISDNIKKAIIATEDENFNDHKGVVPKAVLRAAAGSVLGFGESSGGSTLTQQLL
                     KQQILGDDPSFKRKSKEIIYALALERYMDKDSILSDYLNVSPFGRNNKGQNIAGIEEA
                     AQGIFGVSAKDLTIPQAAFLAGLPQSPIVYSPYTADAQLKSDKDLSFGIKRQKNVLYN
                     MYRTRALTKDEYKSYKDYDIKKDFIKPAVATTNHHDYLYYSALSEAQKVMYNYLIKKD
                     NVSEHDLKNDETRATYRHRAIEEIQQGGYTIKTTINKSVYQAMQDAAAQYGGLLDDGT
                     GKVQMGNVLTDNSSGAIIGFIGGRNYSENQNNHAFDTARSPGSSIKPILPYGIAIDQG
                     MLGSGSVLSNYPTTYSSGEKIMHADEEGTAMVNLQESLDISWNIPAFWTYKMLRDRGV
                     DVKNYMEKLDYPIENFGIESLPLGGGIDTSVAQQTNLYQMIANGGVYHKQYMIESIED
                     SNGKVIYNHESKPVRVFSKATATILQQLLHGPINSGKTTTFKNRLQGLNSGLAGVDWI
                     GKTGTTNSTSDVWLMLSTPKVTLGGWAGHDNNASLAKLTGYNNNANYMAHLVNAINNA
                     DGNTFGKSERFRLDDSVIKAKVLKSTGLQPGVVTVNGRRITVGGESTTSYWAKNGPGT
                     MTYRFAIGGTDSDYQKAWSTLGGKR"
     gene            21039..22298
                     /locus_tag="VBC52_001113"
     CDS             21039..22298
                     /locus_tag="VBC52_001113"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEO6882708.1"
                     /translation="MNIFDELKERGLVFQTTDEDALRKALEEGFVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDVERSLQTKETVVSWGNKIR
                     GQLSNFLEFETGDNKAVLVNNYDWFSNISFIDFLRDVGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFYELNKNYNVTLQIGGSDQWGNMTAGTELIRRKSNGVSHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDADAVRFLKIFTFLSLKE
                     IEDIRIQFEEAPHQRLAQKTLAREVVTLVHGEKAYKEAVNITEQLFAGNIKGLSVKEL
                     KQGLRGVPNYHVQTEDNLNIIDLLVTSGVVNSKRQAREDVSNGAIYINGDRIQDLEYT
                     ISENDKLENEITVIRRGKKKYFVLNFK"
BASE COUNT         7040 a         4475 c         3633 g         7151 t
ORIGIN      
        1 aaaacatgaa agaaaaatta ttagtacctg ctttaaattg tttgctaggt ctactcttcc
       61 taggtgatca ctggttttta tttcgtcttc aaaatggaga actttttgct attgtgacgc
      121 taatactgct ttgcacaact tcatttttaa tcaataagaa tcaccccaaa aatttgactt
      181 taattgctat ggttatcact tttttagccg tattatcttt tctatctacc tttttattag
      241 cttaagccga taaacattat ctccgttgta ttcaacccag ttttcactat aataggcttt
      301 gtcagtcata acagtcttcc ggtagtactt gatattcttt ttgaaagtct ttatattgac
      361 tgaaacctgt tctgttcttt atttttaatt aaaaggtgct aaaatagtat ttgtagaatt
      421 gaggtaatta tgtcagattt atttaacaaa attaaaaccg taactgagct tgatgggatt
      481 gctggctatg aacacaatat ccgcaacttc cttcgtcaag aaataactcc tttagttgat
      541 caagttgaga cagacggact tggtggaatt tttggagtta aaaatactca tgagactaat
      601 gctcctaaag tcatggttgc tgcccatatg gatgaagtcg gctttatggt tagtcatatt
      661 cagccagatg gaacatttcg tgtacttgag gttggaggat ggaatcccct agtagtcagc
      721 tcacaacgct ttaccctcta cacacgttct ggtgatgcta ttcctgttat atcaggctca
      781 gttcctcctc actttcttcg tggacaaagc ggtggaacaa cattacccaa aattagtgac
      841 attgtttttg atggaggatt cacagataaa aatgaagctg aaagctttgg cattgctcct
      901 ggcgatatca ttgttcctaa atctgaaacc attttaactg caaatcaaaa acatattatg
      961 tcaaaagctt gggataatcg ctatggtgtg cttatggtga ccgaattgct aaaaagctta
     1021 aaagatcaaa gtcttagcaa cacacttatt gctggggcaa atgttcaaga agaagtcgga
     1081 cttcgtggcg cacatgtttc aacaactaaa ttcaacccag atatcttctt agctgtcgat
     1141 tgttccccag ctggagatat ttatggggaa caaggcaaaa taggagaggg aaccttaatc
     1201 cgtttttatg atcccggaca tatcatgctt aaagatatga gagatttctt acttacaaca
     1261 gctgaagaag caggtataaa ataccaatat tatgctgcaa atggtggtac cgatgctggg
     1321 gctgctcacc taaaaaatag tggtattcct tctacaacta tcggtgtctg tgcacgctac
     1381 attcattctc atcaaacact ctacgctatg gatgattttc tacaagcaca agcttacctt
     1441 caggccatcg ttaacaaatt agaccgctcg acggtggata ttattaaagg ttattaagat
     1501 agaaaggatg ggagtttcat aaggtagaca cattttagta tctcctttca tccccatatc
     1561 aacattatga aaattggaat tatcggcgtc ggaaaaatgg ccagtgcaat tattcaaggc
     1621 ctcaaacaaa cccaacatga cattatcata tccggatctt gcttagagcg ttcaaaagaa
     1681 attgcagaaa gacttgatgt tacttacgcc gaatctcatc agtcattaat caatcaagct
     1741 gatattatca tgttaggtat taaacctcaa ctatttgaaa aggttcttct tccacttgat
     1801 atcacaaaac caatcatctc aatggcagca ggaatctcct tagcgcgttt aagccaatta
     1861 actcgttctg atttaccatt gattcgtatt atgccaaata ttaacgcaca aatacttcaa
     1921 agttgcacag ctatttgtta caacaatcat gtatcagatg agttacgaca attagccaaa
     1981 gaaattacgg atagttttgg aagtagcttt gatatagcag aaaccaattt tgataccttc
     2041 actgctctag caggttctag ccctgcctat atctatctat ttatagaagc cttagctaaa
     2101 gcaggtgtaa aatatggatt tccaaaagaa caagcactct ctattgtagg acagactgtc
     2161 ttagcttcta gtcaaaacct attacaagga caaaatagca cctctgactt aatcgacaac
     2221 atttgtagcc caggcggaac tactattgct ggccttttag acttagaaaa aaatggtctg
     2281 acgcatagtg ttatttctgc cattgatgct actattgaga aagcaaaaaa actttgagca
     2341 aattgctcaa agttttttta gttattcatc attgtactga ttaacacgct ataagcaaga
     2401 gcattgttaa taaaatgaat cattatacta tattctaagt gctcagtttt ataataaaca
     2461 aaagataaaa tagcacccat cccagcataa ataataaaac tacctatatc agaaggggaa
     2521 tgaataagag caaaagctaa agtaccgatg acaaaacccc atttttgatg tttcgcaaaa
     2581 agttctttag ggataatata gcgaaatata atttcttcca taataggggc accaattatg
     2641 gcaaaaatta acatcaaagc tagtggaagt tgctttgtta attccataat tactgtttga
     2701 tttgctgtat ctttaacccc ttgcatagtc ataatgaggt aaccaataat ttgtggaacc
     2761 ctcaatgata accaacctaa gagaatagta actaatgctt tccttgtcaa taatgctttc
     2821 catctgatta tttgtttaac cttggcataa taaaggaaaa gagcaataat aagtaattct
     2881 aagccaatca acaatatact agtaaaaggc gtttgatagt gattctttag gagccacaca
     2941 gccaatatag gaacaagttg attaataact aaaaatgcta ttagcaaagc taaaattttg
     3001 ccttttctaa taataaatga cacaaaaacc tccaaaataa attattaact tttttctatc
     3061 ataacatatt ttcaaatctt atagacttat ctcacaataa cttctgtaaa ataaagtaaa
     3121 ttaataaata accatataaa ataaaagaga gtatcaatag caagaacttt gcataggaaa
     3181 aagttatacc aaaccagaca ttaatcagac acaataccac taaaacaagg acagataaaa
     3241 ggaaaagaag tcgacaagac atattagcaa taaattgatt acgctcatca aaaactttgg
     3301 tatagtaatg ttgaaatcgc ttttcactaa atgttatcac caaaaaataa aaacttcctg
     3361 caaataaggc aatacctgaa ccaaaggaaa aacctttagt aaaattagat tcaaaggtat
     3421 gtagaaatga tgatactatc ataatcaaac tacatacact aaaaacaaaa gacaaaactc
     3481 tcatatatac tttgtaatcc ttcggagata ctttactatt gggtgacaat aatcctttta
     3541 ttaacataga atactacttt ctaaaacttc aatttaatca tcagctatta tatcgttttc
     3601 ttcaatgaag catagcgata aatagcaaaa atagacatcg caatgctaat actaatagta
     3661 atgataaaaa catattgctg gtaaacgatt ggtggacaaa aatcaatagt ccacatcctt
     3721 gacatattac agattaactt ttaacctttt gtcttttgtg gactgtattt ttccattaaa
     3781 attctatcct ccttcacttt ctgtataaat aaaaacttcc tcaatttgtt tatcaaaata
     3841 acgcgctatc ttaaatgcta gctccaaaga taccgtgtat ttctcttttt ctagagaaat
     3901 aatagtttgc ctagtaactc caagtgcaac tgcaagttct gcttgactta atttcctaga
     3961 ttttcgtaac ttttgtagag aatttttcat atcttacacc ttttttgtat agttagcttt
     4021 acattcatag tatagttaac tatacatttt ttgtcaagta cattttacat atctgttcaa
     4081 aagacaaaaa agttgatagt taaactatca acttttctgg tcttatcaat tttttgataa
     4141 cttatttagc tttaaggcgc tcaacgtcac gcgcaatcat taattcttca tcagtaggaa
     4201 taactaaaac cttcactttt gaatctggtt ttgtgatatc tccaaaataa ccaaaaacat
     4261 ttttttctgg atcaagttca atgccaaacc atgataatcc tgcgataaca tcttgacgca
     4321 ttaatggcgc attttctccc atacctgcag taaaaataat agcatccgcg ccatttaata
     4381 ctgccaagta ttgtccaata aatttcttaa tacggtcaat aaagacatta taggctaaca
     4441 ctgcatttgg atctttactt tgaagacctg cttcaatatc tcgcatatca cttgaagttc
     4501 ctgatactcc aagcaatcct gattgtttgt taagcatatt aacaacagct gctgcatctt
     4561 ccaattctgg atcattagct acaagataag ggataatcgc aggatcaatg tctcctgaac
     4621 gtgttcccat cattggtcct gcaagtggtg tgaatcccat tgatgtatca atagactgtc
     4681 catgataatt tgctgtaata gaaacaccat tacccacatg tgctgtgatt aattttaatt
     4741 cttctaaagg acgtcctaat tgcttagctg cttcttgtgc aacgtattgg tggcttgtcc
     4801 cgtgtgcacc gtatttacgt actttataat cagtatagta cttttgtggg attggatata
     4861 agtaggtatg aggttgcatt gttgtatgga aggctgtatc aaatacacat acactcgtga
     4921 tatctggtaa gatttctcta aacgcacgaa tccctgctgc agctgctggg ttgtggagtg
     4981 gagctaaagc tgataattct tcaacctgtt ctacaacttt atcatctact aaagcagact
     5041 ctttaaaata ttcaccacca gcaacgacac gatgaccaac acctgtaatt tcattaaagt
     5101 ctttgataat accatgtttt gttagatctt ctaaaagaat tttaacagct tgggtatgat
     5161 ctacgatatc taaaatttgt tcatcttttt tatcgtcaaa ttttacagtc gaaatagaat
     5221 ctttcaaacc aatacgttcg ataatccctt tggcaactac tttctcctct ggcatttcat
     5281 ataattgcca tttcaaactt gaacttccag cattaattgc tattgttttt gacataattt
     5341 acctctctaa agcgttttca caatctctat tttatcatat tttttgagta atgactattt
     5401 tctctgctcc atttttgaaa attttcaatg aagtttgcca tattctcacg attttgcaaa
     5461 tctgtcagcg gatatacaaa ggtttctggt ttttgttctg cttgcttctt gagaacaaat
     5521 atagatttcg cattttgacg acttccaaaa atagtttctg gtagagttaa aacggcaatg
     5581 acatctgcat atccttttaa ccactccttc agcaaatcac tttgtggact tgttaaaagg
     5641 ttttcgggtg ctaaaaatat agcgattcca tcttttttca aatatttaag agattgctcc
     5701 atcaatagat ggtgagcata ggtgtgctct ttagaacttg atacagcata tcgtttagca
     5761 ataccatcat taggataata gccaactggt aaatcactaa tgattacatc gctttctttc
     5821 aaaatttgtg gtctaacagc atcctcttgg ataaattggg cactagaacc tataatttca
     5881 gcaatgcttg ctgatagatc aatcaaaaga tcatcaactt caatgcccat ataatttaac
     5941 tctttcgagc tgttattgag gagagtctga gctaaattcc cagttccact tccaatttcc
     6001 aagacatcca ctgtctcttg actcgttaat tcttccaaaa gaaataacaa gataaaacca
     6061 atactatctg gcgtaaactg atgattagct tgtaattgct ctgtttgcgc agatttgata
     6121 aaaatgaact ggaaagtgcg acgccattct tcttgactta agtcaagttg acgtaatttt
     6181 tggttattca ccacaaccat atctaaatca caacttgaac caaggtaata agagttctgt
     6241 tcaattaagg catcataaat atgagttttt aattggttct caatcgtttg gatattttct
     6301 aaaatcagct cataggctgt ctcaattttt tcaaaattca tgatttcctc cagaatttat
     6361 cataacaaaa aacacagcat ctgaaaagag aagcaaacaa aaatcctgtt tttaagcaaa
     6421 accttgtctt actaatattt catcactgta tatttttaat gtttatggct tgctttatta
     6481 tcattagact tgagaggtac ttggagagtg tctgttttac gataaaactt atggttatag
     6541 tttcctccct tatccaatgt aaccttaact tgtaaatagt tcccttcttg gaggtatgaa
     6601 gcatatcctc ctgtaaaata acattttcct gatatggagt caccttctat tgttttataa
     6661 gctaactgcg ctgttaaata agctttactt gcttcaattt ggctatgata ctgtctttct
     6721 gttgccaata ttcttgctag ataaaactgt aagacgagtg taaatacagc agccaataca
     6781 atagcttgca aaaggatgcc agctttaagt ttcttcttta aaatcatagt aaaatgtcct
     6841 ttttaacccg tccttaaaat aaaaaacaag ttttaccata cttttggtct gactcatttg
     6901 acaattgtct aacccataaa ccattggttg ataacctcga ccatcataac ctgtcttacg
     6961 gaaatcatct ttattagatt tgccaaaggt tacaatctta tcttgcttac gtaaataaag
     7021 tttgttctgt cttaaatatt ccagatgagc gccttcaaat tctgcattta gttgctgagt
     7081 taataacacc cattcagact gactggaaga agacatcact actatctgtt gagccaacaa
     7141 ttttgtcagt ccttgataaa ctagtaaagc tcctgtgatt gtaaccaatg ctaccaaaca
     7201 ttttaaaagc gtaaatgctt taacccttaa cttttgatac gtgcaaaatt tccttattat
     7261 cttcaaagag cacaatttta tttttacttt cttcaatacg aatattaaca ccatttgctt
     7321 ctaatatttt ttgatcagtt tgcacagcca ttctagctaa tatcaatact tcttgctcat
     7381 gttgccttct tgcaatatgg gattgttgac gatttatttc acttattact aatcttgtaa
     7441 tagtaactaa aatagctaat cccactaggc tttccagtaa aatataggct ctaattttct
     7501 ttcttacgat aattaccact tcctatatat aattgatacg tgacagtttt tctataacat
     7561 tcaaattgaa tttttgccaa gcttgaattc cctccattag tgtcaaatgc aagtgtatca
     7621 cttttgacta cttttacagt tttaggtaaa taaagtctct cataagtatt ttcgagataa
     7681 ttatgagaga ttgtaagggt ttgtttcttc tgaccaaatg cactcaattt ctgagtatct
     7741 ctataaagat gttcaaagga tatgaagaaa attgtttctt caacctgcct aaaaatatta
     7801 ttaaatgatg ttgaaaatac taacgtcata aatgccacta ctgataatac aataaggctc
     7861 tccaaaacag taaatgcttt aacttgaaat tttctgagaa ccattggaac tgtctttata
     7921 ataatctaaa taggcttttt cttgcttttc agtaatatat cctccagatt ttagagtgga
     7981 taaactagct tttctacctg tttcttgcag ttcaaaaagt tcggcttgac tttctacaac
     8041 cttgacaaca gcggcatttc cagttcttgt gacactttcc ttttgttttg ataaattggg
     8101 tacaaacaat aacaacaaga ccgaaataat gatcaaaaca actaacattt ctaaaagtgt
     8161 aaatgcttta accttcttat ccttacattt taacaataaa tttttcatga taatatctcc
     8221 atattttgat acattggtaa cagcattgct gcataaatca taacaatgat aagagctaca
     8281 aaaataaaaa taacgggttg gattaactgg gtcgctctag ctaattttgt aaaaaaatcc
     8341 tcccacttct catcagcata tatatctaac tctgttccta atttcgcctt aacttggcca
     8401 tattcaatca ttaaactaag ctcagttaag aaaaacggat agtctaatac tttttggtga
     8461 aatgctttac ctgatagaaa accttcttcc atgtcatatc ctatttccct aaaaagtttg
     8521 gatttctgat tttgcattac tttaacaatt tggtccaatt caataccttg acttaataaa
     8581 tttccccact cacgggcata gtaagcagtt aaataaagct taacatatga tccaactaag
     8641 ggaattgttg ttaaaaaaca agctacttta atgcgcgaca atcgcttttg aataatataa
     8701 aatattaaac taaaaataag tacaactgct aaaagtaata agaaaatatt cggcacattt
     8761 gtaatcagtc tagttgcaaa attattttct cctaattggg gcattaaata attcctaagg
     8821 ccaatcataa ttagcaccag aaaagacaat aatatcaatg ggtaagtagc tacttctatt
     8881 actttttttc taactaacaa aagattagct aaataagact caatctttag taggctcctt
     8941 gaaatgttac catgcaaatc agctaatgca acctgtgtga taacagtgtc agaaaaacct
     9001 aactctccta acatttttga taaatctttt ccctctaata atgccttatt catactatct
     9061 gtataacaat cagacaataa cttactcctc tttaagaagg tgaccatatc agttaaagaa
     9121 aaaccactag caaaaagatt attaaaaagt tggactactt tccgttgttt cttgagggat
     9181 aattttttcg acttgtgcct gtttcttact gatatgtcct tcttcagcca agatatccac
     9241 ttgtctattc cacttgtctg atgagtgttt tttaaaatta cctgtctcaa agtcaattag
     9301 gcttcctcct ccaattaaac gttgatatgc tattaatttt agactatttt ctaactcttg
     9361 atagttaacc cctaattcta taagcctatc atagactccg ggaatacttt tagcatgaat
     9421 agtagaaaaa accatcactc ccgttaaact tgcacgaata acagcacggg ccgtcgcttg
     9481 atctctaatc tctccgataa ttaaaatatc tggacgatgc cgtaaagaca gtttgattaa
     9541 agcatcataa gtcattccaa tatcctcatt caattggagt tgtaacatct tgtcattctt
     9601 gatttctacc ggatcttcaa tcgtgataat ttgcttattt ttaaatactt ctgaagctaa
     9661 ttgatacatg agagttgttt taccactccc cacagggccg gaaaaaagat atagccctct
     9721 tatacccagt acttccttca tttgctttat attatcaaac caatatttta agtcctgatg
     9781 acctgaatac aaaatacgaa taactaaaga ttcttgacca cgataatctc ccacactcga
     9841 tagtcgtaat gaaaccagtc ttccctctga cagttcatag tcacaagaac ctaattgact
     9901 tcgtcttttt tctccaacgt tcatgcctgc cacaaattta aagtgactaa taagactagc
     9961 catcctatta aactcaaaaa catcaataaa ccgcctttca tcatcaatac gcatatagag
    10021 ttcataacaa tcacctttgg gaatgatata aatatcttga gcatttactt ctactgcctg
    10081 atgaatgact tgctttgcta atgattgaac catcccgtaa cctccttaac catatattcg
    10141 aaaataaaat aaaaaaatct caccaattgg tgagatcttt tcaatgattt cttgagaggt
    10201 agtaacataa atattatgaa gcagaggctg aacaaaccta ttatatcccc ataaaaacta
    10261 tctaactttt ttaaactact tttaattaca aatttaaaga acatgccgat ggcaactcag
    10321 ctatcttatt aaatacttca aatcgctcaa tagattcttc cttaggaatt tcttgccatt
    10381 ctttcaataa cattaaggaa tgatactgtt ggaggtactc atcacattct tcacaccaac
    10441 gtttttcttt tatactccaa aacgttgcca acaaactaga cttactttga aaataaggcc
    10501 acttttcctg tcccctatcc cactcctctt gaaaatagag caaagcttct gataaggtat
    10561 catactcagc aatttcagta atatcttcct cccaaccttc aataaaccac catggttccc
    10621 aatcaccaaa catttttaca acttgataca taatacactt cctttagaga gtattatata
    10681 aaaaataaga ataaaataaa aaatatctgc tcattccaaa ttcttgtaaa aaaagaacct
    10741 gagttaaact caagttcttt ttgattaatc ttctgttgta ggtgttgata cttccgtaac
    10801 ttctgcatca acaggagttc cttcaataat ctcaacttca ttaacagcaa gtggttcaat
    10861 attacggtaa cgagccatac cagtacccgc tggaatgatt ttaccgataa taacattttc
    10921 tttaagacca agtaagtgat ctttcttacc acggatagca gcatctgtaa ggacacgtgt
    10981 tgtttcttgg aaggatgcag ctgataagaa tgaattagtt tcaagggaag ccttagtaat
    11041 acccataagg acaggacggc ttgtcgcagg gattccacca gaaataacaa tatctttgtt
    11101 agcatctgta aagtctgaaa tatccataag tgtacctgga agaagatctg tatctcctgg
    11161 atccatgaca cgaactttac ggagcatttg acgaaccatt acctcaacgt gtttatctcc
    11221 gatttctacc ccttggctac ggtaaacttt ttgtacttca gcaagtaggt acgtttcaac
    11281 agataaagta tcacgaactt caaggagacg ttttggttgg atagatcctt cagttaaggc
    11341 cgcaccacga gctacttcat caccaacttc aactttcata cgtgctgtaa atggaacaac
    11401 gtattctccc tcacctgtct gacctttaac gaatactttc ttagtacgtg ttgaagagtc
    11461 ttcttcaatt gcaacgactt cacccttaac ttcagtaata actgcctcac ctttagggtt
    11521 acgtgcttca aagatttctt ggatacgtgg gagaccttgt gtaatatcag tatttgaagc
    11581 aacaccaccg gtatggaagg tacgcattgt aagctgtgtc cctggttcac cgatagattg
    11641 agctgcaata gtaccaactg cttcaccaac ctcaacagca tcacctgttg ccaagttaat
    11701 accgtaacag tgacgacaaa caccatggcg agtgttacat gtaaatactg atcggatagt
    11761 tacttcctca acgcccgctt taacaacttt agcagccata tcttcagtaa ttaaggtatc
    11821 tgcaccaacg agaatttcac ctgtctcagg atgtttgatt gacttcttag tataacgtcc
    11881 tatcaaacgc tcttcaagtg tctcagtaac ctctttacca tctgtaattg ctgtgattgt
    11941 caaaccgcgg tctgttccac agtcatcttc acgaataata acatcttggg caacgtcaac
    12001 caaacgacga gtaaggtaac ctgagtcggc tgtcttaagg gccgtatcag tcataccttt
    12061 acgcgcaccg tgagttgaga agaacatttc caaaacacta agaccttcac ggaagtttga
    12121 taagattggc aattccatga tacgtccgtt aggagcagcc atcaaaccac gcataccggc
    12181 aagttgtgag aagtttgaga tgttaccacg agctcctgag tccatcatca taacgatagg
    12241 gttctttgga tcttgtgtct caataagacg tttttcaagt gcttctttag cttcacgcca
    12301 tgttgttgta acagcaacat aacgatcttc ttcagtcatc aaacctcgac ggaaggcttt
    12361 attgatatct tcaactctgt ggtgagctgc atcaataatc tcagctttat tgtcaataac
    12421 tgggatatca gcaataccca cagtcaaacc agcaagtgtt gaatgataat atccaaggtc
    12481 cttcaaacgg tcaaggaaag cagaagtttc tgttgtacgg aaacgtttaa aagtttctgc
    12541 gataatatta ccaagatttt tcttcttaaa gggaatatta atctctaagt tatcaataac
    12601 tgcttgaata tcctgacctg gctctaagaa gtatttatct ggagtttttt ctgtcaagtt
    12661 tgcattatta ggttcaatta agtacggtaa atcttcaggc atgatatcgt tgaagagaat
    12721 cttaccaact gttgtaacca tgattttatg tttttgctct tctgtccaag gcttattcgg
    12781 cattgaatca acagcgatac caacacgtgt atgtaaatga acataaccat tttgatatgc
    12841 cataactgct tcgtcatgat ctttgaaaat catgccttca ccttcacgac cagcatcttc
    12901 catagtcagg tagtaatttc ctaaaaccat gtcctgagat ggtgttacga ctggcttacc
    12961 atctttaggg ttaaggatgt gctctgcagc aagcatcaaa agacgagctt ctgcttgggc
    13021 ttcttcagac aatggaacgt ggatggccat ttggtctcca tcaaagtcag cattgtaggc
    13081 ctcacataca agtgggtgaa gacgaagcgc cttaccatca ataaggacag gttcaaacgc
    13141 ttggatacct aatctgtgaa gagtaggtgc acggtttaga agtactgggt gctctttaat
    13201 aacttcttct aagatgtccc aaatacgctc gtccccacgt tcaaccatac gtttagctgc
    13261 tttaacgttc cctgcgaggt cacgagcaac gatttcacgc attacaaaag gtttgaagag
    13321 ctcaatagcc atttcacgtg gtacaccaca ttggtacatt ttgagtgttg gaccaacagc
    13381 aataacggaa cgtccagaga agtcaacacg tttaccaagc aagttttggc ggaaacgacc
    13441 ttgtttacct ttaagcatat ggcttaatga tttcaaagga cgactacctg gtccggtaat
    13501 tggacgacca cgacgaccat tgtcgataag cgcatcaaca gcttcttgta acatacgttt
    13561 ttcattttga acgatgatgc caggagcatt taattccaac aaacgtgcca aacgattgtt
    13621 acggttaata acacggcggt aaaggtcatt caagtctgat gccgcaaaac gaccaccatc
    13681 taattgaacc atcggacgaa gatccggtgg gataaccggt aaaatgttaa gaaccatcca
    13741 ttctggttta tttccagatt ttttaaaggc atctaacaca tccagacgac gaactgcttt
    13801 cacacgtttt tgacctgtcg ctgatttcaa ctcctcttta agaactgcga tttctgcatc
    13861 taaatcaacg cgtttaagaa gatcttgaat ggcttcagca cccattttag caacaaacga
    13921 accgtaaccg tactcttgaa gtttttcacg gtattcacgc tcagtaagga gtgacttcgg
    13981 ttctaatggt gtgtccattg gatcgataac aacataagcc gcaaaataaa taacttcttc
    14041 aagtgcacga ggactcatat ccaatgtgag ccccatacgc gatgggatac ctttgaaata
    14101 ccagatatga gatactggtg cttttaactc gatatgtccc atacgttcgc gacgaacttt
    14161 tgcacgggtt acttcaacgc cacaacggtc acaaatgatt cctttgtaac gaatacgttt
    14221 gtattttcca cacgcacatt cccaatcttt tgttggacca aaaataactt catcaaaaag
    14281 tccttcacgt tctggtttca atgtgcggta gttgattgtt tcaggttttt taacttcacc
    14341 ataagaccat gaacggacct tacttggtga tgctaatgtg atttgcatac ttttaaaacg
    14401 atttacgtca accactagtt ttacctttct tcgatttatc atcatactag cccagctagg
    14461 atttcaataa atcctaggac tagcattgca tatcaattct atattcgctt ctagttataa
    14521 actagataaa tttaatcttc ttgaacgact tcagatactt gttcagcttg tttttcttca
    14581 gcttcttgtt tcacacgtgc tttttcaagg tcatcaacgt gcatcacatc atcatcttca
    14641 ccttcatcaa gatcacggag ttcaacttcg ttgtcatctt catcaaggac acgcatatca
    14701 agaccaagtg attgcaattc ttttacaaga acacggaatg attctggaac acctggtttt
    14761 ggaattggct taccttttgt gatagcttca taagctttaa gacgtcctgt aacatcatca
    14821 gatttgtatg ttaaaatttc ttgaagaaca tttgaagcac cataagcttc aagcgcccaa
    14881 acctccatct caccaaaacg ttgtccacca aattgagctt tacctccaag tggttgttga
    14941 gtaacaagtg agtatggtcc tactgaacgg gcatgaagtt tatcatcaac catgtggtga
    15001 agtttgatca tgtacatgac accaactgac acacggttat cgaatggttc accagtacga
    15061 ccatcataaa gaacagtctt agcatctgaa tccatacctg cttcttgaac agtttcccaa
    15121 aggtcttctg aagaagcccc atcaaagact ggcgttgcaa tatgaatacc taaattacga
    15181 gcagccatac ctaaatgaag ctccataact tgtccgatat tcatacgtga tggcacccca
    15241 agtgggttca acatgatatc aactggcgtt ccatctggta agtaaggcat atcttcaaca
    15301 ggaacaatac gtgagacgac acctttgttt ccgtgacgac cggccatctt atcaccgact
    15361 ttgattttac gtttttgagc gatataaacg cggacaagca tattaacacc tgattgcaat
    15421 tcatcaccat ttgcacgagt aaagatttta acgtcacgaa ctactccatc gccaccgtga
    15481 ggtacacgta gtgaagtatc acgaacttca cgtgatttat caccaaaaat ggcatgcaag
    15541 agacgttctt cagcagataa gtccttttca cccttaggtg ttaccttacc aacaagaatg
    15601 tcaccttctt ttacctcagc accaatgcgg ataattccca tttcatcgag atcacgtagt
    15661 gaatcttcac caacatttgg aatttcacga gtaatttctt caggcccaag ctttgtatca
    15721 cgcgtttcag attcgaattc ttccaagtga acagatgtat agacatcttc tttcacaagg
    15781 cgctcactca tgataacggc atcctcgaag ttataacctt cccaagtcat ataagcaaca
    15841 actgggtttt gtccaagagc catttcacca ttttccatag atggtccgtc ggcgataaaa
    15901 tcaccttttt caacgagatc accaacttta actaaggtac gttggttata agcagtacct
    15961 gagtttgaac ggcggaattt ttgaacatga taaacatcaa gagaaccatc ttcgcgacgc
    16021 acttcaactt tttcagcatc tgaaaaaata acacgaccat catgtttagc aatcacagct
    16081 gcacctgaat cgtgggctgc ttgatactcc ataccagtac caacatatgg tgcttttgga
    16141 tcaatcaatg gcacagcctg acgttgcatg ttggcaccca tcaaggcacg gttagagtca
    16201 tcattttcca agaaaggaat acatgctgtt gcgacagcaa ctacttgctt aggtgacacg
    16261 tcaacaaagt caacaatgct tgaaggaaac tcttggttat taccttgatg acgacccata
    16321 acgatttctt ctgcaaatgt accgtcttcg ttaagcttag agttggcttg tgcaactgta
    16381 aattcatctt cttcatcagc agtcaaccaa acaatttcgt tcgttaccgc accagtagaa
    16441 cgatctactt tacgatatgg tgtttgaata aatccatatt tattgaggtg tccaaatgaa
    16501 gataagttat taatcaaacc aatgtttgga ccttcaggtg tttcaatcgg acacatgcgc
    16561 ccatagtggg tatagtgcac gtcacgaact tcatatccag cacggtcacg tgtcaaacca
    16621 ccaggtccta aggcagagag acggcgtttg tgcgacaatt ctgatagagg gttgtgttgg
    16681 tccatgaatt gtgacagctg tgatgaacca aagaattctt taacggcggc agtcactgga
    16741 cgaatgttaa tgatttgttg aggtgtcaag acttcattgt cttgtacaga catacgttcg
    16801 cggacattgc gttccatacg agcaagtccg atacggaatt ggttcgcaag taattcacca
    16861 actgcacgaa tacgacggtt acctaaatgg tcaatgtcat ctaccttacc gataccctct
    16921 gcaaggttta agaagtatga catctctgct aaaatatctg caggagttaa ggcacgaact
    16981 ttatcttcag gatttgagtt tccaacaata gtaacaacac ggtctggatc agttggagca
    17041 acaactttga atttttgaag aataactggt tcagtaacta cagcataatc atttggtgtg
    17101 tagacaaact tgtttaagtc tccatcgata tgctctgcaa ttgaatcaat gacatctcgt
    17161 gtcataacgg ttccagcttc agcaagaatc tctcctgttt caccatcaac taaattttca
    17221 gcgattgttt ggttcaatag gcgtgtttta agattcagtt ttttattaat cttataacga
    17281 ccaacagccg ctaagtcata acgacgtgga tcaaagaaac gtgcaactaa aagactacgt
    17341 gagctgtctg cagtttttgg ttcacctgga cgaagacgtt catagatttc tttaagtgct
    17401 tcatctgtac gagaatcact tggattttta tgaatgtctt tttcaatagt attgcgaaca
    17461 agttcactgt caccaaagat gtcaacaatt tcatcgtctc cagagaaacc aagtgcacgg
    17521 actagtgttg tgaatggaat tttacgtgtg cggtcaatac gtgtataagc aatatctttt
    17581 gcgtctgtct caagttccaa ccaagctccg cggttaggga taacagttga cccataacca
    17641 acttttccat ttttatctac cttatcatta aagtaaacac caggagaacg gactaattgt
    17701 gaaacaatga tacgttcacc accattgatg atgaaagtac ccatttcagt cataattgga
    17761 aaatcaccaa agaaaacttc ttgagtttta atttcaccag tttctttatt aactaaacgg
    17821 aaagtaacaa aaataggtgc tgagtagcta gcatcatgga tacgagcttc ttccaaagta
    17881 tatttaggtt cttttaattc ataaccaaca aattcaagat ccatagtatc tgtaaagttt
    17941 gaaattggaa gtacatcctc aaatacttct ttcaagccag catcaagaaa atcttggaat
    18001 gaatcagttt gaatttcaat taagtttggt aaatcaagaa cttccttaat tcttgaaaag
    18061 ctacgacgtg tacggtgttt tccgtactga acttcatgtc ctgccaagtt tttagctcct
    18121 ttttaaagat agagtgacta agtttttatc tcataaaact tagtgagttt atttcatttg
    18181 ctcgcctaac aagaacaagc ttatagatgt cgtttgcggt aattttcaga aagtttaaat
    18241 atgtattaca ataacctttt ttcttaaaat taggcacaaa aagagcagct aaatctcact
    18301 ttatcaagtt gatttaactg ctgtatatct aggtcggaca atatttcaac ctagacagac
    18361 gacaaatagt tacatacatt ataacttatt ttcaccaata aatcaagttt tattctcttg
    18421 acttcaagga agcttctaga atttcaattt gttcatcaac acccttgagt tttagcataa
    18481 cacgatgatt tttaaacata gcatactggt gtttttctaa ttctttgaaa gaagactata
    18541 caagaattaa atcatattat cttggaaaaa ataaaaggag aacagatcac tctattctcc
    18601 tcaaataaaa ctatcagtat cgtaaacctg ttatcgtttt ccacccaaag tagaccaagc
    18661 tttttgataa tcactatcag ttcccccaat agcaaaacga taggtcatcg ttccaggtcc
    18721 attttttgcc caataacttg ttgtgctttc acctccaaca gtgattcgac gaccgtttac
    18781 tgttactaca cctggctgta aacctgtaga cttaagaact ttagctttta taacgctatc
    18841 atcaagtcta aacctttctg acttaccaaa agtattgcca tctgcattat taatagcatt
    18901 aaccaagtga gccatataat tagcattatt attgtaacct gtcaatttcg ctaaagaggc
    18961 attattatca tgtcctgccc atccacctaa agtcacttta ggtgtagaaa gcatcaacca
    19021 aacatcagaa gttgagttag tagtacccgt ttttccaatc caatctaccc cagctaaacc
    19081 actatttaaa ccttgtaagc gatttttaaa ggttgtagtc tttccggagt taattggtcc
    19141 atgtaatagc tgctgtaaga tagtagctgt tgctttcgaa aagacacgga caggtttact
    19201 ttcgtgatta taaatcactt tcccattact atcctctatg ctttctatca tatactgctt
    19261 atgataaaca ccaccatttg caatcatttg ataaaggttt gtttgctgag caactgacgt
    19321 atcaatacca ccaccaagtg gtaaactttc tataccaaag ttttcaattg gatagtctaa
    19381 tttctccata tagtttttta catcaacgcc tcgatctcgt agcatcttgt aagtccagaa
    19441 agctggaatg ttccaagaaa tgtccaatga ctcttgaagg ttaaccatag cagtaccttc
    19501 ttcatccgca tgcataatct tttcgccact tgaatatgtc gttggataat ttgaaagaac
    19561 actaccactg cctaacatcc cttgatcaat agcaattccg taaggtaata tcggtttaat
    19621 acttgaccct ggagagcgtg ccgtatcaaa agcatggtta ttttgattct cactatagtt
    19681 acgaccacca ataaatccta taatagcacc actagaattg tccgttagta catttcccat
    19741 ttgaaccttg ccagtaccat catctaacaa ccctccgtat tgagctgcag catcctgcat
    19801 ggcttgataa acagatttat tgatagtagt tttaatagta taccctccct gttgaatctc
    19861 ttcaatagcg cggtgtctgt acgtcgctct agtctcatca ttttttaaat catgctctga
    19921 aacattatcc tttttaatga ggtaattgta catcactttt tgagcttcag ataatgccga
    19981 gtagtaaaga taatcatgat gatttgttgt cgccactgca ggtttgataa aatctttttt
    20041 gatgtcataa tctttgtagg atttgtactc atctttggtt aaagctctcg ttctatacat
    20101 attgtataac acattttttt ggcgctttat accaaaacta agatctttat ctgatttcaa
    20161 ctgggcatct gcagtataag gagaatatac aataggactt tgcggtaaac ctgcaaggaa
    20221 agctgcctga ggaattgtta aatcttttgc ggaaactcca aaaatccctt gtgctgcttc
    20281 ttcaatacct gcaatattct gtcctttgtt attgcgacca aacggtgata catttaaata
    20341 atctgataaa atcgaatcct tatccatgta acgctctaat gctagagcat agataatttc
    20401 ttttgatttg cgtttaaatg acggatcatc tcccaaaatt tgctgcttta ataattgttg
    20461 tgtcagcgtt gaaccaccgc tactctctcc aaaccccaga acagatcctg ctgcagcacg
    20521 tagtacggct ttgggaacta cacctttatg gtcattgaaa ttttcgtcct ctgttgcaat
    20581 gatagccttt ttaatattat cagatatagc atcctttgcc actggtgtac gttgtaaatc
    20641 agtgtcaatc tctgaaattt gtgatttatc agaataagtt aacctagaca ccctagtaag
    20701 tgtattaact tgtgtcacta aactattttt acttggtact ttaacagagt caacttgact
    20761 agcaagatac cctactgcaa gtcctactcc gagcataaca aatagtgaaa taataacata
    20821 gatgaaatct gataaaagct ttacaattct taaaaaaata gaaccaaatt caagtggtgt
    20881 gtaatcacct aatttagaac tattcaactt cttattacct ttaaacatga ataatacctc
    20941 atcctacatt atatcagatt taatataact tgcatatcct aaagagtttt gatatagttg
    21001 acattgatac acaacaagtt aataattagg agaaaaagat gaatattttt gatgaactca
    21061 aagaacgtgg actcgttttt cagacaactg acgaagatgc tttacgcaaa gctcttgaag
    21121 aaggttttgt ctcttactat actggctacg atcctacggc tgacagcctt catcttggac
    21181 atttagttgc tattttaacc tctcgtcgct tacaacttgc tggtcacaag ccgtatgcac
    21241 ttgtgggagg cgcaactgga cttattgggg atccttcatt taaagatgtt gagcgcagtt
    21301 tacaaacaaa ggaaactgta gttagttggg gtaataaaat ccgcggtcaa ttatctaatt
    21361 ttctagagtt tgaaacaggt gataacaaag ccgtattggt aaataattat gattggttca
    21421 gcaatattag tttcattgat ttcttacgag atgttggtaa atattttact gtcaattata
    21481 tgatgagtaa agaatctgtt aaaaaacgta tcgaaacagg gatttcctat actgaatttg
    21541 cttatcaaat tatgcaagga tatgactttt acgaattaaa taagaactat aatgtcactt
    21601 tgcaaattgg tggttctgat caatggggca acatgactgc tggaaccgaa cttattagac
    21661 gaaaatcaaa tggtgtatct catgttatga ctgttccatt gataacagat tctacaggaa
    21721 aaaaattcgg aaaatctgaa ggaaatgctg tttggcttga tgctgacaaa acctctcctt
    21781 atgaaatgta ccaattttgg ctcaatgtta tggatgctga tgccgttcgc ttccttaaga
    21841 tttttacatt cctaagcctt aaagaaatag aagatatacg tattcaattc gaggaagcac
    21901 ctcaccaacg cttagcacaa aaaacactag cacgtgaagt tgtaactctt gttcatggtg
    21961 aaaaagctta taaggaggcc gttaatatca cggaacagct atttgctggc aatataaaag
    22021 gattgtctgt taaagagctc aaacaaggtt tacgtggagt accaaattat cacgttcaga
    22081 cggaagataa cctcaatatc atagaccttc tagtcacttc aggagttgtc aattctaaac
    22141 gtcaagctcg agaagatgtc tccaatggag ctatttatat taatggagat cgtattcaag
    22201 atttagaata tactatctca gaaaatgata agcttgaaaa tgaaattaca gtcattcgtc
    22261 gtggaaagaa aaaatacttt gtattaaact ttaaataga
//
LOCUS       DATRNK010000029        22107 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.029, whole genome shotgun sequence.
ACCESSION   DATRNK010000029 DATRNK010000000
VERSION     DATRNK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22107
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.029"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            103..435
                     /locus_tag="VBC52_001114"
     CDS             103..435
                     /locus_tag="VBC52_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882709.1"
                     /translation="MKNLFATTEASSRKQEQDRIVNYIKQHVELTNGDQIKKVEFIDF
                     QKNEMTGTWGISTKINEQFSISFSEDRIGGKLRALGYQPNEIGFSKDINSNNQNINDI
                     EVIYMKKE"
     gene            437..1644
                     /locus_tag="VBC52_001115"
                     /pseudo
     CDS             437..1644
                     /locus_tag="VBC52_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1632..2033
                     /locus_tag="VBC52_001116"
     CDS             1632..2033
                     /locus_tag="VBC52_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882710.1"
                     /translation="MGKLDSNEDKLSNSKRDLEKLEDDYHHKTMAISNKFFELEDKRT
                     EFETMLQETYEATSYNLRQDENINEESFMTMNHIIDAFQSDFDTEYTKEKRRLTALEE
                     ETNQKYSKKRQLLEEKIDHLMSERRDYGNPW"
     gene            2017..2322
                     /locus_tag="VBC52_001117"
     CDS             2017..2322
                     /locus_tag="VBC52_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882711.1"
                     /translation="MGILGDYVLSEASDYISGKKDIKALKKKLDKMLVTGVYAPRIKS
                     KRSSIVSTYDEEITTTATNAKASITAIAGQIDTAIKGQFRTKVETVLDNNSTKYDEI"
     gene            complement(2511..2583)
                     /locus_tag="VBC52_001118"
     tRNA            complement(2511..2583)
                     /locus_tag="VBC52_001118"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2548..2550),aa:Lys,seq:ctt)
     gene            complement(2846..4114)
                     /locus_tag="VBC52_001119"
     CDS             complement(2846..4114)
                     /locus_tag="VBC52_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEO6882712.1"
                     /translation="MADNLALRMRPRNINEVIGQQHLVGNGKIIDRMVAANMLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGNIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKKAIQ
                     LAISDKERGFPFLVTIDDEALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDGSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPEAQIHTVTALEAAQRIGFPEARILIANIVVDLALSPKSNSAYL
                     AMNAALADLRRSGNLPIPRHLRDGHYSGSKTLGNARDYKYPHAYPEKWVKQQYLPDKL
                     VGHNYFEANETGKYERALGSNKERIDKLSD"
     gene            complement(4107..4598)
                     /locus_tag="VBC52_001120"
     CDS             complement(4107..4598)
                     /locus_tag="VBC52_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001879696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6882713.1"
                     /translation="MIIRPMQDCDRGAVLTLQNTGWTALTSPVYDRKWTESDLEKNLA
                     NGMSFFVAEVDDKIAGILDFGPYYPFPAGKHVATFGILIAEPYQGQGLGKALLKALLT
                     EAKAQGYIKIAMHVMGNNSRAISLYQKYGFTEEARITKAFFIENHYVDALIFAKDLEN
                     NHG"
     gene            5203..5391
                     /locus_tag="VBC52_001121"
     CDS             5203..5391
                     /locus_tag="VBC52_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6882714.1"
                     /translation="MNRVKDYRLETKKSQLALAKEIGVSRQTINMIENNKYNPSLDLC
                     IKLAKALGTDLNALFWED"
     gene            5394..5921
                     /locus_tag="VBC52_001122"
     CDS             5394..5921
                     /locus_tag="VBC52_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEO6882715.1"
                     /translation="MNKENFTDRLIKRFYGISGPLDEYKRREADRMGNIALIFLVWFL
                     MLGNVVALLLAEDFPQEVAMYYPMILELLVFILFGYLVYASNKTQLTKYDEDELSDKE
                     RKSIRHIGWKSGIYFGVFMYLTSYTWIDSPLSYFDYLLSGKALFTLVFTGSFFGISTA
                     VVNYFNRRKGKEERE"
     gene            5995..6465
                     /locus_tag="VBC52_001123"
     CDS             5995..6465
                     /locus_tag="VBC52_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEO6882716.1"
                     /translation="MAKFGFLSVLEEELDKHLNYDFAMDWDKKNHALEVTFVLEAQNS
                     SAIETVDDQGETSSEDIVFEDYVLFYNPVKSRFDAEDYLVTIPYEPKKGLSREFLAYF
                     AETLNEVATEGLSDLMDFLTDDSIEEFGLSWDTDAFENGRAELKETEFYAYPRY"
     gene            6508..6816
                     /locus_tag="VBC52_001124"
     CDS             6508..6816
                     /locus_tag="VBC52_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEO6882717.1"
                     /translation="MKSGQKIIELRLYDERRQTITIGDYIVFYLLNDKRKKLTTEVVC
                     LHIFDDFSQLYESLDLLKCGYTLDDIETARPEEMERYYPPEQIRKYGALGIELRLLHG
                     "
     gene            6809..7267
                     /locus_tag="VBC52_001125"
     CDS             6809..7267
                     /locus_tag="VBC52_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6882718.1"
                     /translation="MDSFDELFDFSGAKIALFCEGKILTSLRDDFPDLPYAGFWDLPG
                     GGREDNETPLECLFREVDEELNLTLTRNHINWVKTYRGMLKPDKLSVFMVGHISQKEY
                     DSIVLGDEGQGYKLMSIDEFLSHKKVIPQLQERLRDYLEVEDDYFTKGGS"
     gene            7239..7709
                     /locus_tag="VBC52_001126"
     CDS             7239..7709
                     /locus_tag="VBC52_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6882719.1"
                     /translation="MITLQKAEASDLEKIIAIQRASFKVVYEKYHDQYDPYVEEVEQI
                     RWKLVERPDCFYHFVLVDETIVGFLRLVIKDEEKRAWLGTAAILPQYQGQGYGSAGMA
                     LLEKTYPKLTKWDLCTIAQEKLMVSFYEKCGYHSTHTEPEQEGMDMVYMTKKIE"
     gene            complement(7748..8464)
                     /locus_tag="VBC52_001127"
     CDS             complement(7748..8464)
                     /locus_tag="VBC52_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6882720.1"
                     /translation="MKTVKEVSILSGVSVRTLHHYDKIGLFPPTALSEAGYRLYDDEA
                     LIRLQEILLFRELEFPLKDIKYLLEQAKEERQDLLAQQIKLLEWKRLHLEQVITHAKR
                     LQEKGDDYMNFDVYNKTELEQLQAEAKEKWGQTAAYQEFAQKHASDDFAQISQEMAKI
                     MVQFGQLKTQNVSDESVQMCVKRLQDYISQHFYTCTNEILAGLGQMYQSDDRFSQSID
                     KAGGAGTSEFVSQAIAYYCQ"
     gene            8588..9046
                     /locus_tag="VBC52_001128"
     CDS             8588..9046
                     /locus_tag="VBC52_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HEO6882721.1"
                     /translation="MEIVELLREWYSSFEVKEEKWNEKYEGILIRIEGDRNYKRCRLA
                     HKTASKMGYFTVFWQKDGNGKNIPFYEQDEVGDLIIVIKDGRRKGLFIIPKEVAVSKG
                     ILSSANSQGKMAMRFYPPWCSDLNRTALVTQRWQLNYFIDLSRNNEGVTT"
     gene            9043..9996
                     /locus_tag="VBC52_001129"
     CDS             9043..9996
                     /locus_tag="VBC52_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEO6882722.1"
                     /translation="MNTWNELTVHVNREAEEAVSNLLIETGSQGVAISDSADYLGQED
                     RFGELYPEVEQSDMIVITAYYPDTLDIEAVKADLADRLANFEGFGLATGSVNLDSQEL
                     VEEDWADNWKKYYEPARITHDLTIVPSWTDYEAKAGEKIIKLDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVIDVGTGSGVLSIASSLLGAKDIYAYDLDDVAVRVAQENIDMNP
                     ATENIHVAAGDLLKGAHQEADVIVANILADILIHLTDDAYRLVKDEGYLIMSGIISEK
                     WDMVRESAEKAGFFLETHMVQGEWNACVFKRTDDISGVIGG"
     gene            9996..10736
                     /locus_tag="VBC52_001130"
     CDS             9996..10736
                     /locus_tag="VBC52_001130"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEO6882723.1"
                     /translation="MQQYFVNGKADDFVTIEDKDTIKHMFNVMRLTEDDQVVLVFDDT
                     IKRLAKVVDSSAHRFQILEELDSNVEMPVQVTIASGFPKGDKLDLVTQKATELGAAAI
                     WAFPADWSVVKWDGKKLAKKEDKLAKIAMGAAEQSKRNRLPQVRLFEKKADFQAELNG
                     FDKIFIAYEESAKEGEMSALAQNLQTVKAGDKLLFIFGPEGGISPKEIAAFEEAGAIK
                     VGLGPRIMRTETAPLYALSVISYSAELL"
     gene            10733..11320
                     /locus_tag="VBC52_001131"
     CDS             10733..11320
                     /locus_tag="VBC52_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6882724.1"
                     /translation="MIIAIQGGMAVGKTTALKIIEKVYPKIAISFEDISPVVDKINKH
                     QLDNNKLDDYCEIQQLFIQHERDRYELVRDNEIVLMDYGAEEIAFYTQYFPKVAGYSW
                     TLPSNLLLEVNRLKECFADYIIFLDADKTVLYQRQKNDFNRRRHFFKWYTHHLLPAKK
                     HYLDTFSNVIHINTNHLNAEEVAEKIIDIIRKLTL"
     gene            11521..12402
                     /locus_tag="VBC52_001132"
     CDS             11521..12402
                     /locus_tag="VBC52_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEO6882725.1"
                     /translation="MKKHKMLSLLAVSGLMGIGILAGCSNDSSSSPKGTINIVSREEG
                     SGTRGAFIELFGIESKNKKGEKVDHTSDAATVTNSTSVMLTTVSKDPSAIGYSSLGSL
                     NSSVKVLKIDGKNATVKDIKSGSYKISRPFNIVTKEGKEKEATKDFIDYILSKDGQAV
                     VEKNGYIPLDNAKAYQAKVSSGKVVIAGSSSVTPVMEKIKEAYHKVNAKVDVEIQQSD
                     SSTGITSAIDGSADIGMASRELDKTESSKGVKATVIATDGIAVVVNKKNKVNDLSTKQ
                     VKDIFTGKTTSWSDLSK"
     gene            12417..13262
                     /gene="pstC"
                     /locus_tag="VBC52_001133"
     CDS             12417..13262
                     /gene="pstC"
                     /locus_tag="VBC52_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO6882726.1"
                     /translation="MKQTKEGLMQAVFFITACVSVISAILICLFLFSSGLPAITKIGW
                     GNFIFGKVWHPSNSIFGIFPMIVGSLYVTAGALLLGGPIGILTAVFMAYFCPENIYKP
                     LKSAINLMAGIPSVVYGFFGLVVIVPMIRQYIGGFGMGVLAASILLGIMILPTIVSIS
                     ESSLRAVPESYYEGGIALGASHERSVFFAVLPAAKRGILASVVLGIGRAIGETMAVIM
                     VAGNQAVLPQSLTSGVRTLTTNIVMEMGYSSGLHRQALIGTAVVLFIFILMINISFSA
                     LQKEE"
     gene            13264..14142
                     /gene="pstA"
                     /locus_tag="VBC52_001134"
     CDS             13264..14142
                     /gene="pstA"
                     /locus_tag="VBC52_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO6882727.1"
                     /translation="MENINQKTSKQYLASLKKDPLSFFLFAIVYLGAILSFATIAFVV
                     IYILVKGLPHVNTGLFAWTYNTQNVSLLPAFINTIFIIALTLLFAVPLGIGGSIYLTE
                     YARRDNPYLKIIRVATETLAGIPSIIYGLFGALFFVKYTHLGLSLISGSLTLSIMILP
                     LIMRTTEEALLSVPDSYREGAFALGAGKLRTIFKIVLPSAMSGIFAGIILAVGRIIGE
                     SAALIFTAGTVAKVAHSVFSSSRTLAVHMYAISGEGLYVDQTYATAVILLLLVIIVNF
                     VSGLVAKRLGSESNSE"
     gene            14135..14884
                     /gene="pstB"
                     /locus_tag="VBC52_001135"
     CDS             14135..14884
                     /gene="pstB"
                     /locus_tag="VBC52_001135"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6882728.1"
                     /translation="MSKLVINNLDLYYGEFHALKDVNLDIEEKEITAFIGPSGCGKST
                     LLKSINRMNDLVKNCKITGDITLEGEDVYRQLDINQLRKKVGMVFQKPNPFPMSIYDN
                     VAFGPRTHGIHSKAELDDIVERSLKQAALWDEVKDRLHKSALGMSGGQQQRLCIARAL
                     AIEPDVLLMDEPTSALDPISTAKIEELVIQLKKNYTIVIVTHNMQQAVRISDKTAFFL
                     MGEVVEYNKTSQLFSLPQDERTENYITGRFG"
     gene            14881..15537
                     /gene="phoU"
                     /locus_tag="VBC52_001136"
     CDS             14881..15537
                     /gene="phoU"
                     /locus_tag="VBC52_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO6882729.1"
                     /translation="MIRSRFASQLNDLNKEIIFMGALCEDIIGKSLGALTNSNDVYLD
                     DISETYHKIEQMERDIEERCLKLLLRQQPVAKDLRRISSALKMVYDMKRIGAQAYEIA
                     EIVSLGHIIQGAGSERDQLNSMSNNVISMLTKSIDAFIYDNEEQAHQVIEQDRTVNQE
                     FDTIKKQLVLYFSVQDVDGEYPIDVLMIAKYLERIGDHTVNIAKWVLFSITGNLDGEA
                     "
     gene            15537..16214
                     /locus_tag="VBC52_001137"
     CDS             15537..16214
                     /locus_tag="VBC52_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6882730.1"
                     /translation="MIYCVEDDADIREMMLYTLQMAGFTAQGFSSSELFWEAIQEKVP
                     DLILLDIMLPGDDGLTILERLRRKHQTEMIPVIMTTAKGSEYDKVKGLDLGADDYLVK
                     PFGMMEMISRIKAVLRRSRQVDSKAHIIIGNLEIDPTNYWVKRGTEKIHLTLKEFELL
                     VLFFRNPNRVFTRQELLDKVWGEQFLGETRTVDVHIGTLRTKLGEDGYLIATVRGVGY
                     RLEERHD"
     gene            16207..17862
                     /locus_tag="VBC52_001138"
     CDS             16207..17862
                     /locus_tag="VBC52_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEO6882731.1"
                     /translation="MTKKIFRTTLSASLGIVLVTILMIMGFLYNYFNRIQREQLRTQT
                     ALASQGISFEGKDYFENLKTSNVRITWVDNKGQVLYDTQSDAKHMKNHANRQEIKEAI
                     KSGYGESTRWSATLTEKSIYAAQRLNNGTIVRLSVAQQTIFYLLLGMMSPLAIIILLA
                     IILSVLIARYIAKKVSEPLNNIDLDHPLSNDSYEEITPLLRRLDSHQSKIQHQKLLLQ
                     KRQKEIDTIISKIKEGMILLDDQARIVSINAEALKLFQINDDWHGRFMMEVSRDLTLK
                     DLIDQGLKGKKKEANIDIENNHYRVLVRPTTDNNRVTGLVVLLFDVTDQLQMEQLQRE
                     FTANVSHELKTPLHVISGYSELLANQMVPNEEVPQFAAKIHKESERLVKLVEDIINLS
                     HLDEQEKLPQETVNLYDLTQKVLEGLQAKADKKHIQINFNGEEAILRGNPVLLNSLVY
                     NLCDNALTYNHEKGQVNVTLKNSPDTITLEVSDTGLGIAEKDKKRIFERFYRVDKSRS
                     KIVGGTGLGLSIVKSALDFHNGSIKVDSHLGQGTTMTVLLHKQ"
     gene            18020..20203
                     /locus_tag="VBC52_001139"
     CDS             18020..20203
                     /locus_tag="VBC52_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO6882732.1"
                     /translation="MKNNVKQLFSFEFWQKFGKALMVVIAVMPAAGLMVSIGNSISLL
                     DPSNVLLGRIANVIAQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLSFILINLI
                     TGNFFGVKTDMLADSKATVQTVFGATIRVSDYFVNVLGQPALNMGVFVGIISGFVGAT
                     AFNKYYNYRKLPDALTFFNGKRFVPFVVIYRSVIVALILSVFWPVVQSGINGFGKWIA
                     SSQDSAPILAPFVYGTLERLLLPFGLHHMLTIPMNYTQLGGTYTVLTGATKGAQVLGQ
                     DPLWLAWVGDLINLKGSNSSQYHHLLTSVTPARFKVGQMIGASGILMGLSYAMYRNVD
                     KDKKLKYKSMFISAAAATFLTGVTEPIEYMFMFAAMPLYLVYAVVQGCAFAMADIVNL
                     RVHSFGNIEFLTRVPMGIKAGLGGDIFNFVWVTLLFAVLMYFIANFMIKKFNLATAGR
                     NGNYDNEEVDNAPSTASGSADANSQVVQVINLLGGRDNIEDVDACMTRLRVTVKDGNS
                     VGSEAAWKKAGAMGLVLKGNGVQAIYGPKADVLKSDIQDLLDSGTVIPIVDLETGQPV
                     AAAQVTTYKGITEEIVSVANGQVEALDVVKDPVFSQKMMGDGFAVEPTDGNIYVPVSG
                     TVTSVFPTKHAFGLLTESGLEVLVHIGLDTVALDGQPFEVKISSGQKVVAGDLAVVAD
                     LEAIKAAGKETSVIIVFTNVSDIKTVKLEKSGPQIAKTVVAKVEL"
     gene            20242..21072
                     /locus_tag="VBC52_001140"
     CDS             20242..21072
                     /locus_tag="VBC52_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEO6882733.1"
                     /translation="MKGYSMAKCLTLNTHSWMEVNALKKLFDLAEHIFREKYDIICLQ
                     EVNQSISSPLAKSSPNYHPIEGTPALHQDNFALQLVHYLNLQGLHYHWTWAYNHIGYS
                     KYHEGVAILSLKPLKPDDILVSAVDDETDYHTRRALVAETTLNDKVVTVVSLHLSWFE
                     KGFAEEWKRLETTLLEVETPLLLMGDFNNPTGNQGYELVLNSPLALKDSHQIANHVFG
                     DHTIMADIDGWEGNKKALKVDHIFTSEDLSISSSQVVFEGGEAPVVSDHYGLEITLNL
                     "
     gene            21288..22040
                     /locus_tag="VBC52_001141"
     CDS             21288..22040
                     /locus_tag="VBC52_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6882734.1"
                     /translation="MNIFILEDNFIQQARLERIIEEVKIRRSIHSKMVNTFEKPVQLL
                     ESICEKGMHNLYFLDIEIKNDEQRGLEVAKQIRQIDPYAQIVFVTTHSELMPLTFRYQ
                     VSALDYIDKGLSQEAFSRRIEEVLLYANNACSKNLLEDSFYFKSRYSQVQVPFNDILY
                     IETSLRSHRVVLYTAKDRMEFTATLGDILKQEPRLFQCHRSFLVNPLNIFKVDRSNKL
                     IYLKNGSNCLISRNKIKDIMVVIEENQRGRGI"
BASE COUNT         7086 a         3539 c         4655 g         6827 t
ORIGIN      
        1 ctatatttga atggtataga aaaaacagag gtttatttgc ttatgaaaaa acgtatatgg
       61 tatttgataa taataatcac agtaatttta ggaggactag ccatgaaaaa cttatttgca
      121 acaacagaag catcatcaag gaaacaggaa caagatagaa ttgtcaatta cataaaacaa
      181 catgttgagt taacaaatgg tgatcaaata aaaaaagttg agtttatcga ttttcaaaaa
      241 aatgagatga caggtacatg gggaatttct actaaaatta atgaacaatt ttcgattagt
      301 ttttctgaag atagaattgg tggtaaactt agagcattag gatatcaacc gaatgaaata
      361 ggtttttcaa aggacatcaa tagtaataat caaaatatta atgatattga agtgatttat
      421 atgaagaaag aatagaatga tttcagaaga aagttacaga tatttagttg aggatgctta
      481 tcgagttgat tcaaaaaaag taaaaatacc tctgaaaaga ggtgatattg taggaaacag
      541 tgactatgtt attatagaac cacctataga taatacttca aatggtatgc aagctatggt
      601 agttgctccg ataaaagaag gaacgacagc tgaacagata ctagtgaaat agtgattgct
      661 tatgcaggta ctaatttagg tgatcgttta gacatagcta cagatgttga aatggttgct
      721 ggaggggata catatttact tgctgaccct aaaaccaaga cttttaggaa atctcaggga
      781 aaatcagcac tagagtatgc cgaaaaaata tcgagtaaat atcctaattc agaaattact
      841 acaaccggtc actccttagg ggaatcagag gcactatatg tcgctttaaa aatgggttgg
      901 atgaatgttg gttataatgg tccagatatt cataacatga tatcagatga cgaaatatat
      961 tatatgaaat ctcatccaga acaatttaga aattatcgaa atacactaga tgttatcgga
     1021 aatattacag ggaacgaaac aaaaacagct atttattatg ataaagttga aggtatttat
     1081 ggacctttaa aagctcatgg attagcaaat tggagtttta ataaggaagg tcaagttgtt
     1141 gatgaaaatg gcaaagaaat tacgaatggt atgatacttt cagttgcaca aacaactgta
     1201 aaaacttttc aaattaataa gcagaagcaa cagatgatga agggaggtct aaattctaat
     1261 gaaaaaatct tcttggatgc tgaacaagct gctgttattg caagtggttt agtctctact
     1321 gctgaaactg ctttagagga tatcaaaacc tcagcacaat ctgctgttga agaagctgaa
     1381 gaattgtgga ataccactaa aagcatgccc tttggtatta cagagcttac ggaagcagag
     1441 ttggcagaag cctatgcaga aggtggtgtt acctatgaga gcatagtaac taaaacagat
     1501 acccattttg ataaaaaagt cgctaaagca gaagaattgg taactactta cacgactttg
     1561 aaaagtgata ttcaatctgg tgttgaaact atgttgtcta aggatagtga attggctgga
     1621 gattttaaag catgggaaaa ctagattcga atgaggataa acttagtaac agtaaaaggg
     1681 atttagaaaa gttggaagat gattaccatc ataaaaccat ggctatctct aataaattct
     1741 ttgaactgga agataaacga actgagtttg aaacgatgct tcaggaaact tatgaagcaa
     1801 caagttataa tcttcgtcaa gatgagaaca ttaatgaaga gtcctttatg actatgaatc
     1861 acattataga tgcttttcaa agtgattttg atacagaata tactaaagag aaaagaagat
     1921 tgactgcctt agaagaagaa actaatcaaa agtactctaa aaagagacaa ttgcttgaag
     1981 aaaaaataga tcatttaatg tcagaaagga gagactatgg gaatccttgg tgattatgtt
     2041 ttatcagaag cctctgatta tatcagtggc aaaaaagata tcaaggcttt aaaaaagaaa
     2101 cttgataaaa tgttagtgac aggtgtttac gctccaagaa tcaagtctaa acgtagtagt
     2161 attgtctcta cttatgatga agagataacg acgactgcaa caaacgcaaa agcaagcata
     2221 acagcaatag caggtcaaat agatactgca attaagggac agtttagaac taaagttgaa
     2281 actgtattgg ataataacag tacaaagtat gatgaaattt aaaagacatg ggctaaataa
     2341 tttagctctt tttttatact caaaaataag agaaatacaa agcaaaatac atatttatgc
     2401 aagtaaagag aaataaaaaa atcaccattt tatctgtcaa aaaaaattgg tgggcaagtg
     2461 taaaaagagc gtcaacaaag cctataggta taggctttgt tttataagac tgccccctac
     2521 agggctcgaa cctgtgaccc atagattaag agtctactgc tctaccaact gagctaagga
     2581 ggctaggaag ctgtattggc accgatgatc cactttttgt attgatcccg cacaaaataa
     2641 gcaggtgggt aactcgcacc tctttaagtg gcttccgcgt gttaacggct tgcgcactag
     2701 agatgacttc gtttcccgaa atacaaagaa atagtcggtc aacacttaga tgtggattcg
     2761 tatgccacag caatgtttct atatctttat gataccactt tttataaaat tttcaagcaa
     2821 aattatcgat ttttaaaact ggcaatcagt ctgagagctt atcaatgcgc tctttatttg
     2881 aaccaagtgc gcgttcgtat ttaccagttt cattggcttc aaaataatta tgtcctacta
     2941 gtttatctgg taagtattgt tgtttaaccc atttttcagg ataggcgtga gggtacttgt
     3001 aatcccttgc attgcccaag gttttactac cgctgtaatg tccatctctt aggtgacgtg
     3061 gaataggtaa atttcctgaa cgacgtaaat cagctaaggc tgcattcata gccaaatagg
     3121 ctgaatttga tttaggtgat agagctaaat ccacaacaat gttagcaata agaatacgag
     3181 cttctggaaa accaatgcgt tgggcagctt ccaatgcagt cacagtatgt atttgtgctt
     3241 cggggttagc taagccgata tcttcataag caataatggt caaacgtctt gctaaactag
     3301 gtaaatctcc agcttctact aaacgagcgg catagtgcag gctggcattg acatcagaac
     3361 ctctaattga tttttgtaag gctgatagga catcataatg cccatctccg tttttatcca
     3421 ttgtaatata actacgctgc agactatttt ccattgtctc tagactaata tgacggctac
     3481 catcttcatt aggagaagta gacatgactg ctagatccaa ggaattataa gcagatcgta
     3541 ggtctccatt tgtggcggta acaatgaaat ctagcgcctc atcatcaatt gtcactaaaa
     3601 aaggaaatcc ccgttcttta tcggaaattg ctagctgaat ggctttttta atatcctcgt
     3661 tagaaagagg ttctaactca aaaatttgta ccctacttct aatggcagga gttactgaaa
     3721 agaaaggatt ttcagttgtt gctccaatca taataatatt gccattctca agtaaaggta
     3781 aaagaaaatc ttgttttgtc ttgtcaagtc tatgaatctc gtcaagtaat aaaactaagc
     3841 caccagaaaa tttagcttct tctgcaattt cttgaagacg ttttttacta tcaacagtgg
     3901 cattaaaagt acgaaaggca tatttagtag taccagcaat agcagaagca atagaagtct
     3961 tcccaatacc tggtgggcca tataggatca tggaagataa catattagct gcaaccattc
     4021 gatcaataat tttgccatta ccaacgagat gttgctgccc gataacttca ttaatattgc
     4081 gaggacgcat acgtagagca agattgtcag ccatgattat tctccaaatc ttttgcgaaa
     4141 attaaagcat caacataatg attttcaatg aaaaaggctt tagtgattcg agcctcttcg
     4201 gtaaaaccgt atttttgata gagggaaatg gctctactat tgttgcccat gacgtgcatg
     4261 gcaatcttga tgtaaccttg agctttagcc tcagttagca gagctttcaa gagagcctta
     4321 cctagcccct gaccttggta gggttcagcg atgagaatgc cgaaagtggc aacatgcttg
     4381 ccagctggga aaggatagta gggaccaaag tccaagatgc cagcgatctt atcatcgacc
     4441 tcagcgacaa agaaggacat gccatttgcc agattcttct ccaagtcact ttcagtccac
     4501 ttgcggtcat agacaggaga ggtaagagca gtccagcctg tattttgtaa ggtcagaact
     4561 gccccacggt cgcagtcctg cataggtctg ataatcataa ttgatgatac aaagggacgg
     4621 ttcggtttac cgaaaatttt cgtaaagaaa tagggaacga cgaagtgtcg tcagacacta
     4681 ggaagttcat cttttcttct agaaaattag tcccgtattc aaatcctttc tatttttata
     4741 tagagttttt cttttagctg actcttttaa tatagtatga agccgaggct gggcaaaaac
     4801 ttctaggcct cttaattttt tgtagtctga tttgcaagac acagtggttg agtgggttct
     4861 aattcgttga taaatcaagt tttacagccc tactcaactg tgcggaggtg ggagtgaaac
     4921 agtctgggga tagactgttt caggtcagca actgaaaatc aaaacttgct gacaaaatcg
     4981 aattcctttg gaattaccga tttttgtccc actctcattg taacataaaa taaaaaaatt
     5041 tgagaaaagg tgtctaatat agttgacaat ccctttcaaa agattataat aaaactatca
     5101 aatatagttg acacatttta ggccaacaaa attttttaag caagcgtgtc gtatatatat
     5161 gacatgctct tgtgaaacct taccgcaata gaaaggagat agatgaatag agttaaggat
     5221 tatcgtttgg aaactaagaa gtctcagctt gctttggcga aggaaatcgg agtttctaga
     5281 cagacgataa atatgattga gaacaacaag tataatccct cgcttgattt atgtatcaag
     5341 ctggctaagg ctttagggac tgatttgaat gcactgtttt gggaggattg aaaatgaaca
     5401 aagaaaattt tacagaccgc ttgattaaac gtttttacgg tatttctggc cctttggatg
     5461 agtacaagag acgtgaagcc gaccgcatgg ggaatatagc tcttatcttt ctggtatggt
     5521 tcttgatgct tggaaatgtt gtagcgctcc tattggcaga ggattttccc caagaggttg
     5581 ccatgtatta tccgatgatt ctggagctac ttgtttttat cttgtttggt tacctcgtct
     5641 atgctagcaa taagacacag ttgacgaagt atgatgagga tgaattgtcc gataaggagc
     5701 gaaaaagtat acgtcatatt ggttggaagt caggtatcta ttttggtgtt ttcatgtatt
     5761 tgacttctta cacttggata gatagtccgc ttagctattt tgattatcta ttatcaggta
     5821 aagccttgtt caccttagtc tttacaggta gcttctttgg tatatcgacg gctgtcgtca
     5881 attattttaa tcgacgaaaa ggtaaggaag agcgtgaata gcttcttgaa agagttggga
     5941 agaccagctc ttttttgcta taatgaaaga aagtaaattt gtaaaggatt taaaatggct
     6001 aaatttggat ttttgtctgt tctggaagaa gaattggaca agcacttaaa ctatgacttt
     6061 gccatggatt gggacaagaa aaatcacgct cttgaggtga cctttgtcct agaagcgcaa
     6121 aacagctctg ctattgaaac cgtggacgac cagggggaga cgagtagcga ggacatcgtt
     6181 tttgaggact atgtactctt ttacaaccct gtcaagtctc gctttgatgc tgaggactat
     6241 ctggtgacta tcccctatga gcctaagaag ggcttgtcgc gtgagtttct ggcttatttt
     6301 gcggagactc tcaacgaggt tgccactgaa gggttgagcg acctcatgga cttcctcacc
     6361 gatgacagta tcgaagagtt cggtctttct tgggatacgg atgcttttga aaatggcaga
     6421 gcagaactga aagagacaga gttttatgca tatccgaggt attgagataa agcatgaaat
     6481 gatgttagca ccggaaccat ttgaaaaatg aaatcaggtc agaaaataat tgaactcagg
     6541 ttatatgacg aacgaaggca gactattaca attggtgatt acattgtctt ttatctgtta
     6601 aatgataaaa gaaagaaatt gaccacagaa gttgtttgtt tacatatatt tgatgatttt
     6661 tctcaacttt atgaaagttt agatttgcta aaatgtggat atacattgga tgatatagaa
     6721 acagccagac cagaggagat ggaacgttat tatccccctg aacaaattag aaaatatgga
     6781 gctttaggaa tagaattgag gttattgcat ggatagtttt gatgagttat ttgatttctc
     6841 aggtgcaaag atcgctcttt tctgtgaagg aaagattttg accagcctaa gagatgattt
     6901 tccagatttg ccttatgcag gcttttggga cttaccagga ggtggtcgtg aggacaatga
     6961 aacacctttg gaatgtcttt ttcgagaagt tgatgaagaa ttaaatctga ccttaactcg
     7021 aaaccatatt aattgggtca agacttatag gggtatgttg aagcctgata aattatcagt
     7081 ttttatggtt ggacatatca gtcagaaaga gtacgatagc attgttctag gtgatgaagg
     7141 acaaggctac aagctcatgt ccattgacga atttctaagc cataaaaagg tcattcctca
     7201 gttgcaggag aggttgagag attatttgga ggtggaagat gattacttta caaaaggcgg
     7261 aagctagtga tttagagaag attattgcca ttcagagagc tagctttaag gtagtctatg
     7321 agaaatacca cgatcaatac gatccctatg tggaagaagt tgagcagatt cgatggaaac
     7381 tagtggagcg accagattgt ttctatcatt ttgtccttgt tgatgagaca attgttggtt
     7441 ttcttagatt ggttatcaag gacgaggaga agcgtgcttg gcttggtacg gcggctattc
     7501 ttccccaata tcaagggcag ggctacggct cagcagggat ggctttatta gagaaaacct
     7561 atcctaagct tacaaagtgg gatttatgta cgattgcaca agaaaaacta atggtcagtt
     7621 tttatgaaaa atgtggttat cattcaaccc ataccgaacc tgagcaagaa ggaatggata
     7681 tggtctatat gacaaagaag atagaataaa aaaagaaaac tagatgattg tctagttttc
     7741 tgatagttta ttggcaatag taagcaatag cttggctgac aaattcactt gttcctgctc
     7801 caccagcttt gtcaattgat tgactaaacc ggtcatctga ttgatacatt tgtcctagtc
     7861 cagcaaggat ttcattggta caggtgtaaa aatgctgaga gatatagtct tgtagccgtt
     7921 tcacacacat ttgaacagat tcatcagata cattttgtgt ctttaattgc ccaaattgta
     7981 ccattatttt tgccatttct tggctaattt gtgcaaagtc atctgaagca tgtttttgag
     8041 caaattcttg gtaggctgct gtttgtcccc atttttcttt tgcttcagct tgtaattgtt
     8101 ctaattctgt tttattataa acatcgaaat tcatataatc atctcctttt tcttgtaatc
     8161 gtttcgcatg ggtgataacc tgctcaagat gcaatctttt ccattcaagc aatttgattt
     8221 gttgggctaa taagtcttgc ctttcctctt ttgcttgctc aagcaggtat ttgatatctt
     8281 tgagagggaa ttccaactca cgaaatagca aaatttcttg caggcgaatc aatgcttcat
     8341 cgtcataaag tcgatagcct gcttcggata gggcggtagg tgggaataaa ccgattttat
     8401 catagtgatg caaggtgcgc acacttacac cgcttaagat actgacttct ttaactgttt
     8461 tcatggtaat aacctcctct cacttacata gtataaagga taacctaagg tcagggtcaa
     8521 gcaaaaaata aattattttg aaaagtatgg ttaatcatat tgatttgaag ataaaggagg
     8581 gatagttata gaaatagttg agctactaag ggaatggtat tcttcctttg aagttaaaga
     8641 agaaaagtgg aatgaaaaat atgaaggtat cctcataaga attgaaggcg atagaaacta
     8701 taaaaggtgt cgtttagctc ataagacagc tagcaagatg gggtatttta cagtattttg
     8761 gcagaaagat ggaaatggta aaaatattcc tttttatgaa caagatgaag tgggagattt
     8821 gattattgtc attaaagatg gtaggaggaa aggtttgttc atcattccca aagaagttgc
     8881 agtgagtaag ggtattcttt catctgctaa ctctcaagga aagatggcta tgcgatttta
     8941 ccctccttgg tgtagcgatt taaatagaac agctttggtg acacagcgat ggcagttgaa
     9001 ctattttatt gatttaagta gaaataatga aggagttact acatgaacac atggaatgaa
     9061 ctaacggttc acgtgaatcg tgaggcagag gaagctgtct cgaatttatt gattgaaact
     9121 ggcagtcagg gggttgccat tagcgattcg gctgactacc ttggacagga ggatcgtttc
     9181 ggtgagcttt atcctgaggt ggagcagtct gatatgatcg ttattactgc ctattatcct
     9241 gatacgcttg atattgaggc ggttaaggct gatttggctg atcgtttagc taattttgaa
     9301 ggctttggtc ttgcgacggg tagcgtcaac cttgacagtc aggaattggt cgaagaggac
     9361 tgggctgata actggaagaa gtactatgag ccagctcgca ttacccatga tttgaccatc
     9421 gtgccgtcat ggacggacta cgaggctaag gctggtgaga agattatcaa gctggatcct
     9481 ggtatggctt ttgggactgg gactcaccca acgactaaga tgagtctttt tgccttggag
     9541 caagtgcttc gtggtggtga gacggttatc gatgtgggga ctggttctgg cgttttgtct
     9601 attgctagct ctctcttggg tgccaaggac atctatgctt atgatttgga tgatgttgca
     9661 gtgcgcgtgg ctcaagaaaa tatcgacatg aacccagcta ctgaaaatat tcatgtggct
     9721 gctggtgact tgctcaaagg tgcccaccaa gaggcagatg tcattgttgc caatatttta
     9781 gcggatattt tgatccatct gacagacgat gcctaccgtt tggtcaagga tgagggttat
     9841 ctcatcatgt ctggtatcat ttctgaaaaa tgggatatgg tgcgtgagag tgctgaaaaa
     9901 gctggtttct tccttgagac tcacatggtt caaggtgagt ggaatgcctg cgttttcaaa
     9961 aggacagatg atatttcagg agtgattggc ggctaatgca acagtatttt gtaaatggca
    10021 aagcagatga ctttgtgacc atcgaggaca aggataccat caagcacatg ttcaatgtca
    10081 tgcgattgac agaagatgac caagtggtct tggtttttga tgataccatc aagcgtctgg
    10141 caaaggtggt ggacagctct gcccatcgct tccaaattct agaagagcta gacagcaatg
    10201 ttgaaatgcc tgtccaagtg accatcgcct caggctttcc caagggagac aagctagacc
    10261 ttgtgacaca aaaggcgaca gaattgggtg cagcggctat ctgggccttc ccagctgact
    10321 ggtcagtcgt caagtgggat ggtaaaaaac tggctaaaaa agaagataag ctggcaaaaa
    10381 ttgctatggg cgctgcagag cagagcaagc gtaatcgtct tcctcaggtt cgtttatttg
    10441 agaagaaggc tgactttcaa gcggagctta atggctttga taaaattttt atcgcctatg
    10501 aagagtctgc caaggagggt gaaatgtcag cgctagccca gaacttgcag actgtaaaag
    10561 caggtgacaa gctcctcttc atctttggac cagaaggggg catttctcca aaagaaatcg
    10621 cagcttttga agaggctgga gcgataaaag tgggcctggg gccacgcatc atgcgcacag
    10681 aaacagcacc gctctatgct ctaagtgtta ttagttactc agcggagtta ttatgattat
    10741 tgctattcaa ggtggtatgg ctgtagggaa aaccacagct ttgaaaatca ttgagaaagt
    10801 ttacccaaaa atcgctatta gttttgagga catttctcca gtagttgata agattaataa
    10861 acatcagctt gataacaata aattagacga ttattgtgaa attcaacaac tatttattca
    10921 gcatgaacga gatagatacg aacttgttag agataatgaa atagtgttaa tggattatgg
    10981 agctgaggaa attgcttttt atacacaata ctttccaaag gtggcgggtt atagttggac
    11041 tttaccttct aatttgttac tagaggtaaa taggctaaag gaatgttttg ctgattatat
    11101 tatttttcta gatgcagata agacggtcct ataccaacgt caaaaaaatg attttaatag
    11161 acggcgtcat tttttcaaat ggtatactca tcacctgttg cctgctaaaa aacattattt
    11221 agacacattt tccaatgtta ttcatattaa tacaaatcat ttaaatgctg aggaagtggc
    11281 tgaaaaaatc attgacatca ttcgtaagtt aactctataa tgggatagtt atactccaat
    11341 tatgtggtat aacaactgtt tcaatccgac ttactggctg aattaccata atagaaaaaa
    11401 ggagtttgga taattttcca actcttttta attttacatt gattttacaa aaatcctcaa
    11461 agagatttga tatttcttaa ctataataaa atcaaatcaa aaaatattta ggagttcatc
    11521 atgaaaaaac acaaaatgct atctcttttg gcggtatctg gtcttatggg aattggcatc
    11581 ctagcaggat gctcaaatga ttcatcatct tcacctaaag ggacaattaa cattgtttct
    11641 agagaggaag ggtctggaac tcgtggagct ttcatagagt tatttgggat cgagtcaaaa
    11701 aataaaaaag gcgaaaaagt agaccatact tctgatgcag caactgttac caattcaact
    11761 tcagttatgc taaccactgt ctctaaagac ccgtcagcta ttggttattc ttcactaggt
    11821 tcattaaata gtagcgttaa agtgcttaaa attgatggca aaaatgcgac tgtaaaagat
    11881 attaaatcag gaagctataa gatttctcgt cctttcaata ttgtaacaaa agaaggtaag
    11941 gaaaaagaag caacaaaaga ttttattgat tatatcttaa gcaaagatgg gcaagcagtc
    12001 gttgagaaaa atggctatat tccacttgac aatgccaaag catatcaagc aaaagtgtca
    12061 tctggtaaag ttgttatagc aggttcaagt tcagtaacgc cggttatgga aaagattaaa
    12121 gaggcctatc ataaagtaaa tgcaaaagta gatgttgaaa ttcaacaaag tgattcttca
    12181 actgggatta caagcgcaat tgatggatca gcagatatag gtatggcatc acgtgaattg
    12241 gataagacag agtcatccaa aggggtcaaa gctactgtta ttgcaacaga tggtatagca
    12301 gtagttgtta ataagaaaaa taaagttaat gatctatcaa caaaacaagt aaaagacatt
    12361 tttactggaa agacaacgtc ttggtctgac ttatcaaagt aaggtaagag taaacaatga
    12421 aacaaacaaa agaaggtctt atgcaagctg tgtttttcat cacggcttgc gtcagtgtta
    12481 tatcagctat tttaatttgc ctctttctct tctctagtgg tcttccagct attacgaaaa
    12541 tcggctgggg aaattttata tttggtaaag tctggcatcc tagtaatagt atttttggca
    12601 tctttccaat gattgtaggt tccttatatg ttacagcggg agctttactt ttaggaggac
    12661 cgataggtat ccttactgct gtttttatgg cttatttctg cccagaaaat atttataagc
    12721 ctttaaagtc agctatcaat ttaatggcgg gaatcccttc agttgtttat ggtttctttg
    12781 gactcgttgt tattgtccct atgataaggc aatatattgg tggctttggt atgggagtct
    12841 tagcagcttc aattttgcta ggaattatga ttttaccaac aattgttagc atttcagaat
    12901 cttctttaag agctgttcca gagagctatt atgaaggcgg tattgccctt ggtgcctctc
    12961 atgaacgaag tgtcttcttt gctgttttac cagctgctaa acgtggtatt ttagcatcgg
    13021 ttgttttggg aattggacgt gctattggtg aaacaatggc tgtcatcatg gtagcgggta
    13081 atcaagctgt cttgcctcaa tcgctgacat caggcgttag aactctaacc actaatattg
    13141 tcatggaaat gggctattct tctggtttgc atcgtcaagc cttaattggt acagctgtgg
    13201 tactctttat ctttatatta atgattaata tcagtttttc agcattacag aaagaggaat
    13261 aagatggaaa atattaacca aaaaacgtca aaacaatatt tagcatcttt aaaaaaagat
    13321 cccctttcct tctttctttt tgctattgtc tatttgggag caattctaag ttttgctaca
    13381 attgcatttg ttgttattta tattttagtt aaggggcttc ctcatgtgaa tactggcttg
    13441 tttgcatgga cttataatac tcagaacgtt tcactattac cagctttcat taacactatc
    13501 tttatcatcg ccttaacttt attatttgca gttccacttg gtattggtgg atcaatttat
    13561 ctaacggaat atgctagacg tgataatcct tatctcaaaa ttatccgtgt tgcaaccgag
    13621 acattggcag ggataccttc aattatttat ggtttatttg gtgcattatt ttttgtaaaa
    13681 tacacacact taggcttatc tttgatatcg ggttctttga ctttgagcat catgatttta
    13741 cctttgatta tgaggacaac tgaagaagct ttactatctg ttccggattc ttatcgagaa
    13801 ggagcctttg cgcttggtgc gggtaaattg agaaccatat ttaaaatcgt attacctagt
    13861 gctatgtctg gtatttttgc tggtattatt ttagcggtag gacgtatcat tggtgagtca
    13921 gcagcactta ttttcacagc tggaacagtt gcaaaagtag cacattcggt atttagttca
    13981 tctcgtactt tagcagtaca tatgtatgct atttcgggtg agggcttata tgttgaccaa
    14041 acttatgcga ctgcggtcat cttattgctt ttagttatta ttgtcaattt tgtatctgga
    14101 ctagtagcca aacgattagg aagtgagtcg aacagtgagt aaattagtaa ttaataattt
    14161 agatttgtat tatggagaat ttcacgctct caaggatgtt aatttagaca ttgaagaaaa
    14221 agaaatcaca gcctttattg gaccttctgg gtgcggaaag tctactctct tgaaaagtat
    14281 taaccgtatg aatgatttgg tcaaaaattg caaaatcaca ggggatatta ctttagaagg
    14341 cgaggatgtc tatagacaat tggatattaa ccaattgcgt aaaaaggttg gaatggtgtt
    14401 tcaaaaacct aatccgtttc caatgagcat ttatgataat gttgcttttg gtccaagaac
    14461 gcacggtatt cattccaaag cagagttaga tgatatcgtt gaacgttccc ttaaacaggc
    14521 ggctctatgg gatgaggtta aagatcgtct tcacaaatct gctctgggta tgtctggcgg
    14581 acagcagcaa cgtttgtgta ttgcgcgtgc tcttgctatt gagcctgatg ttttacttat
    14641 ggatgaaccc acaagtgcct tggatcctat ttcaacagca aaaattgaag aattggttat
    14701 ccaactgaag aagaactaca ctattgttat agtgacacat aatatgcaac aagctgttcg
    14761 tatttctgat aaaacagcat tctttctgat gggagaagtg gttgaatata ataaaacgag
    14821 ccaactcttt tcattgccac aagatgagcg tacagaaaac tatattacag ggagattcgg
    14881 atgattcgtt cacgatttgc aagtcaactt aatgacctta ataaagaaat tatttttatg
    14941 ggggcattgt gtgaggacat cattgggaag tccttaggtg ctctaacaaa tagtaatgac
    15001 gtttaccttg acgacatttc tgaaacttat cacaagattg aacaaatgga acgggatatt
    15061 gaggagagat gtttaaaact tttacttcgt cagcaaccag ttgccaagga tttacgccgt
    15121 atttcttctg cattaaaaat ggtttatgat atgaaacgta ttggtgctca agcttatgaa
    15181 attgcggaaa ttgttagttt aggtcatatt atccaaggtg caggttctga aagggaccaa
    15241 cttaacagca tgtctaataa tgtcattagt atgctgacta agagtataga tgcttttatt
    15301 tatgacaatg aagaacaagc gcatcaagta attgaacaag ataggacagt taaccaggaa
    15361 tttgatacaa taaagaaaca gttagtgtta tatttttcgg tacaggatgt tgatggtgaa
    15421 tatcccatag atgttttaat gatcgccaag tacctagagc gtattggtga tcatacagta
    15481 aatattgcta aatgggtgct tttctcaata acaggtaacc tagatggaga agcgtaatga
    15541 tatattgtgt tgaagatgat gctgatattc gcgaaatgat gctctatact ttgcaaatgg
    15601 caggttttac agctcaagga ttctcgagtt cagagctttt ttgggaagct attcaggaga
    15661 aagttccaga cttgatttta cttgatatta tgttgccggg agatgatggt ttgactattt
    15721 tagagcgttt aaggagaaaa catcaaacgg aaatgatacc agtaatcatg acaactgcta
    15781 aaggtagtga gtatgataaa gttaaagggt tagatcttgg agcagatgat tatctcgtca
    15841 aaccttttgg gatgatggaa atgatttcac gaataaaagc agtcttgaga cgtagccgcc
    15901 aagtagattc aaaagctcat attatcattg gaaatttaga gattgatccg actaattatt
    15961 gggtcaaaag gggaacggaa aaaatccact taaccctaaa ggaatttgag ttattagtat
    16021 tattcttccg taatcccaat agagtcttta caaggcaaga acttctggat aaggtttggg
    16081 gagaacaatt tttaggagaa actagaactg tggatgttca cattgggaca ctgcgaacaa
    16141 aacttggtga ggatggctat ttgatcgcta cagtaagggg agttggatac cgtttggagg
    16201 aaagacatga ctaaaaaaat tttccgtaca actttatccg ctagtttggg aattgtttta
    16261 gttacaattt tgatgattat gggatttctc tataattact tcaatcgcat tcagcgagaa
    16321 cagttgagaa cacaaacggc actggcgtca caaggaattt cctttgaagg taaagattat
    16381 tttgaaaatc ttaaaacttc aaatgtgcgt attacttggg tagataataa gggacaagtg
    16441 ctttatgata cccaatcaga tgcaaaacat atgaaaaatc acgccaatcg tcaagaaatt
    16501 aaagaagcta tcaagagtgg ttatggtgag agcacgaggt ggtcagcgac acttactgag
    16561 aagtctatct atgctgcgca acgtttaaat aatggaacga tagttcgttt atcagttgcg
    16621 caacaaacta ttttttatct tctccttggt atgatgagtc ccttagcaat tatcatctta
    16681 cttgctatta ttttatctgt attaattgca cgatatatag cgaaaaaagt atcagagcct
    16741 ttaaacaata ttgacttaga tcatccttta tcaaatgata gttacgagga gataacacca
    16801 ttattaaggc gtcttgatag ccatcagtct aagattcaac atcaaaaact gttattacaa
    16861 aagcgtcaaa aagaaattga taccattatt tctaaaatta aggaaggaat gattctttta
    16921 gatgatcaag caagaatcgt tagtatcaac gcagaagctt taaagttatt ccaaattaat
    16981 gatgattggc atgggcgctt tatgatggaa gttagccgag atttaactct caaagactta
    17041 attgatcaag gactaaaagg gaaaaagaaa gaggctaata ttgacattga aaataatcat
    17101 taccgcgtct tggtaagacc aactacagat aacaaccgag taacaggatt ggttgttcta
    17161 ctgtttgatg tgactgacca actccaaatg gaacaacttc aacgtgaatt tactgccaat
    17221 gtctctcatg aattgaaaac cccgcttcat gtcatttctg ggtactcaga actgttagct
    17281 aatcaaatgg ttccaaacga agaagtccca caatttgcag ctaaaattca taaagaatct
    17341 gaacgattag ttaaattggt tgaggatatt atcaaccttt ctcaccttga tgaacaagaa
    17401 aaattaccgc aagagacagt aaatctgtat gacttaacac aaaaagtttt agaaggttta
    17461 caagctaaag cagataagaa gcacatacaa atcaatttta atggagaaga agctattttg
    17521 cgagggaatc cagttttatt gaattccttg gtgtacaact tgtgtgacaa tgctctcacc
    17581 tataatcatg agaaaggtca agtaaatgtg actttgaaaa attcacctga tacaattacc
    17641 ttagaagtta gtgatacggg actaggcatt gcagagaagg acaaaaaacg tattttcgaa
    17701 cgtttctatc gcgtcgataa aagtcgctca aaaatagttg gagggactgg tttaggtttg
    17761 tctattgtaa agagtgcttt agattttcac aatggtagta taaaagtgga cagccatttg
    17821 gggcagggaa caacaatgac cgttttactt cacaaacaat aaaaactcac aaataaaagt
    17881 ctagatgata ttatctagac ttttttagta attcagaaaa aatatacaag taagggttta
    17941 caaaaaacaa ataagtgcta taataaaacc ataataacgc aaacgcttgc actattaagt
    18001 gcataaaagg agattgaaga tgaaaaataa tgtcaaacag ctctttagtt ttgaattttg
    18061 gcagaagttt gggaaggctt tgatggttgt tattgctgtt atgccagcag caggattgat
    18121 ggttagtatt ggtaactcca ttagtctatt ggatccatca aatgttttat tagggcgcat
    18181 agctaatgtc attgctcaaa ttggttgggg agttattggt aacttacaca tcttatttgc
    18241 attggctatt ggtggtagct gggctaaaga acgtgccggt ggtgcttttg cggctggctt
    18301 atcctttatt cttattaacc ttattacggg gaactttttt ggtgttaaaa cagatatgtt
    18361 agcagacagt aaagcaactg ttcaaacggt atttggtgca accatcagag tttcagatta
    18421 ctttgttaac gttcttggac aacctgctct taatatgggt gtatttgtcg gtattatttc
    18481 aggtttcgtt ggtgcaactg cctttaataa atattataac tatcgtaaat taccagatgc
    18541 tttaactttt ttcaatggaa aacgttttgt tccatttgtt gttatttatc gttcagtcat
    18601 tgttgcatta attttatcag tattttggcc ggttgttcaa tcaggtatca atggctttgg
    18661 taaatggatt gcaagttcac aagacagtgc tccaatatta gcaccatttg tctacggtac
    18721 gttagagcgt ttattgctcc catttggttt acaccatatg ttaactattc caatgaacta
    18781 cacgcaacta ggtggcacat acactgtcct tacaggtgca actaaaggag cacaggtgtt
    18841 gggacaagat ccactgtggt tagcatgggt tggtgattta attaacttaa aaggctcaaa
    18901 ttcaagtcaa taccatcact tattgacaag tgtcactcca gcacgtttta aagtggggca
    18961 aatgattggt gcttcaggta ttttgatggg attatcttat gctatgtatc gtaacgttga
    19021 taaagataaa aaattgaaat acaagtcaat gtttatttca gcagcggcgg ctactttctt
    19081 gacaggtgtt actgagccaa ttgagtacat gttcatgttt gcagcaatgc cactctacct
    19141 tgtatatgct gtcgttcaag gatgtgcgtt tgctatggca gatattgtta acctccgcgt
    19201 tcattcattt ggtaatatag aatttttaac acgtgttccc atgggaataa aagcgggcct
    19261 tggtggtgat attttcaact ttgtttgggt gacacttctt tttgcagtat tgatgtactt
    19321 tattgcaaac tttatgatta aaaaattcaa tttagcaaca gcaggacgta atggtaatta
    19381 tgataacgaa gaggttgata atgctccttc aacagcttct ggttctgctg atgcaaattc
    19441 tcaagttgta caagttatta atttacttgg tggtcgtgat aacattgaag atgtagatgc
    19501 atgtatgact cgtcttcgtg tcacagttaa agatggaaat agcgtgggtt ctgaagcagc
    19561 ttggaaaaaa gctggtgcaa tgggattggt gttaaaaggt aatggcgttc aagctattta
    19621 cggacctaaa gcagatgtcc ttaaatcaga cattcaagat ttgctagatt caggtacagt
    19681 tattccaata gttgatttag aaacaggcca acctgtagca gcagcccaag ttacaactta
    19741 taaaggaata acagaagaaa ttgtttcggt ggctaatggt caagtagaag cattggatgt
    19801 tgttaaagac cctgtctttt ctcaaaaaat gatgggtgat ggctttgctg tggaaccaac
    19861 tgatggcaat atttatgttc cagtttcagg tacagttact agtgtctttc caacaaaaca
    19921 tgcttttggt ttattaacag aaagtggcct agaagtttta gtacatatcg gtctagatac
    19981 agtagctctt gatggtcaac catttgaggt taaaatttct agtggtcaaa aagtggtagc
    20041 aggcgatctt gctgtagtag cagatttgga agctatcaaa gcagctggta aagagacaag
    20101 tgtgattatc gtctttacaa atgtttcaga tataaaaact gttaaacttg aaaaatcagg
    20161 tccacaaata gctaaaacag ttgtggcaaa agtcgaactg taacaaacga taggagctgg
    20221 gcaagtctca gcttctttct gatgaaagga tactctatgg caaaatgctt aacgttaaat
    20281 actcattcat ggatggaagt aaatgctcta aagaaattat ttgatttagc agagcatatc
    20341 ttccgtgaaa aatatgatat catctgccta caagaggtga accagagcat atcttcccct
    20401 cttgctaaga gtagtccaaa ttatcatccg attgaaggta caccagctct tcatcaagat
    20461 aactttgcct tgcaattagt gcattaccta aatcttcaag gtctacacta tcattggaca
    20521 tgggcttata atcatattgg ttatagtaag taccatgaag gggtagcgat actttctctg
    20581 aaaccattaa agccagatga tattttagtt tcagctgtag acgatgagac tgattatcat
    20641 actcgtcgtg ctctggtagc tgagaccact ctgaatgaca aagtcgttac agtagttagc
    20701 ctccatttat catggtttga gaaaggattt gcggaggagt ggaaacgact agaaactacc
    20761 ctacttgagg tagaaactcc acttttactc atgggtgact ttaataatcc tactggaaat
    20821 cagggttatg aacttgttct taatagtcca ttagcattaa aggatagcca ccaaatagcc
    20881 aatcatgtat ttggagacca tactattatg gctgatattg atggttggga agggaataaa
    20941 aaggctctta aagttgacca tatttttact agcgaagacc tttcaatcag ttcaagtcaa
    21001 gtagtttttg aaggtggtga agcacctgtc gttagtgatc attacggctt ggaaattacg
    21061 ttaaatcttt agattaatct atttaatata ttaaaagcat cctaaatttg tctataaaac
    21121 aaaacttagg gtgctttttg aaaaatatgt cacattattt aacttcttac gaagtttgga
    21181 atgcatgaga aaatatgata aaatgataat aactattata ggtgatttta ataaatattt
    21241 taatcaattg gtttatctat tagaacaatt aatacgagga gacgagagtg aatattttta
    21301 ttttagagga taattttatt caacaggcac gtttagagag aataattgag gaagttaaaa
    21361 ttaggagaag tatacacagt aaaatggtaa atacctttga aaaaccagtt caattattgg
    21421 aatcaatctg tgaaaaaggt atgcataatt tatatttctt agacattgaa ataaaaaatg
    21481 atgagcagag gggcttagag gtcgcaaaac aaattagaca aattgatcct tatgctcaga
    21541 ttgtttttgt tacaactcat tcggaattaa tgcctttaac ctttcgttat caggtatcag
    21601 ctttagacta tatcgataaa ggactttcac aggaagcatt tagcagaagg attgaagaag
    21661 ttttactata tgctaataat gcttgcagta aaaacttact agaagattct ttttatttta
    21721 aatcacgcta tagtcaagta caagtacctt ttaacgatat cctttatata gaaacgtcat
    21781 taaggtcaca tagagttgtc ctttatactg caaaagacag aatggaattc acagccactt
    21841 tgggggatat tttaaaacaa gaacctaggc tctttcaatg tcatcgctct tttttagtaa
    21901 atcccttgaa tatttttaaa gtggataggt caaataaact aatctattta aaaaatggtt
    21961 cgaattgttt gatttctcgt aataaaataa aagatattat ggtagtaata gaggaaaatc
    22021 aaagaggtag aggaatatga attctactct atccatctta gagatagctt taacgataat
    22081 attaacaata tttctctttt ctaaaat
//
LOCUS       DATRNK010000030        21326 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.030, whole genome shotgun sequence.
ACCESSION   DATRNK010000030 DATRNK010000000
VERSION     DATRNK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21326
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.030"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            288..1247
                     /locus_tag="VBC52_001142"
     CDS             288..1247
                     /locus_tag="VBC52_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6882735.1"
                     /translation="MSKVRYGVVSTAKVAPRFIEGVRLAGNGEVVAVSSRTLESAQAF
                     ANKYHLPKAYDKLEDMLADESIDVIYVATINQDHYKVAKAALLAGKHVLVEKPFTLTY
                     DQANELFALAESCNLFLMEAQKSVFIPMTQVIKKLLASGEIGEVISISSTTAYPNIDH
                     VTWFRELELGGGTVHFMAPYALSYLQYLFDATITHASGTATFPKGQSDSQSKLLLQLS
                     NGVLVDIFLTTRLNLPHEMIIYGTEGRLIIPHFWKTTHAKLVRNDTSARTIQVDMVSD
                     FEKEAYHVSQMILEGQRVSHIMTPQLTLSGVKIIEDLYRSWGK"
     gene            1408..2310
                     /locus_tag="VBC52_001143"
     CDS             1408..2310
                     /locus_tag="VBC52_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6882736.1"
                     /translation="MFSSDFGFEIINVDDLSLQEEKFLPSELLAYARDENESSFVRDI
                     EEHLTLVYQLLDTQGHVDDVRHVPRVIPVTLFLKEDGLFVLANHKNINLVKKALNRVE
                     KVDSPKHLLLSLVTAFSKQYFDVLDTISEERDKLINDLRKRPNKSNLARLANLQSGTV
                     HLMMGTKQNFEMLTDLQNIEQDKENTRNEKMQLQDAIIEARQLSNMCSLNSQVFQELS
                     SSYNNVLSNNLNDNVTTLTIISIGISIIAMVTSFYGMNVKLPFDSVDAVWVLIILITT
                     IITIMLSIVMYIYVHKEDNHPKHK"
     gene            2470..3534
                     /locus_tag="VBC52_001144"
     CDS             2470..3534
                     /locus_tag="VBC52_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEO6882737.1"
                     /translation="MSIESDFFRKKRFIFSSLEEFGFIKSDQEYIYCQTFMDNDFKAI
                     ITISLDGKIAGKVIDLALEEEYLPLRAANYNGSFVGEVRSAYMAILGDISDSCCKDLL
                     FTKDQSNRLAEKIAKTFEDSVDYPFAKHPQYASYRVSGKWYALLFPLKMGKLENVPAQ
                     LSEEEVEVLNIKVNPQDMEILLQKEGVYPSYHMSKKTWVSIVLDNTLSDIEIFKLVSD
                     SRKLVSPNKKSNSEPEFWIIPANPKFYDIDKEFAEHQLIDWTQKGKIAVGDYVFIYIT
                     APIRALRYVCQVIERDLKTSTYLGARDVKAYMRLKLIRQFEDSTYPVAFLAEHGVKTV
                     RGPRRMTKELRQVMAKTILE"
     gene            3649..4641
                     /locus_tag="VBC52_001145"
     CDS             3649..4641
                     /locus_tag="VBC52_001145"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEO6882738.1"
                     /translation="MKKSFIHQQQEISFVKNTFTQYLIDKLEIVEVQGPILSQVGDGM
                     QDNLSGIEHPVSVKVLNIPEAEFEVVHSLAKWKRHTLARFGFNEGEGLFVHMKALRPD
                     EDSLDPTHSVYVDQWDWEKVIPDGRRNLDYLKETVEKIYKAIRLTELAVEARFDIESI
                     LPKRITFIHTEELVEKYPDLSPKERENAIAKEYGAVFLIGIGGELADGKPHDGRAPDY
                     DDWTTPSENGFKGLNGDILVWNEQLGTAFELSSMGIRVDEDALKRQVVLTGDEGRLEF
                     EWHKTLLRGFFPLTIGGGIGQSRLAMFLLRKKHIGEVQSSVWPKEVRDTFENIL"
     gene            complement(4686..5135)
                     /locus_tag="VBC52_001146"
     CDS             complement(4686..5135)
                     /locus_tag="VBC52_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001893380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transport accessory protein"
                     /protein_id="HEO6882739.1"
                     /translation="MIKKLLLSLIAILCLTTLKSNLSSADTMTISQQQYQHMITNSDL
                     IAITDKALVSKSQQSEATIFIGRQTCQYCRAFLPKLLKSKATLHSKIYYLDSQKYKGK
                     RLKSFFKKHHITTVPNLAHYQRGKMTKYLVQGSQATPQQIQTFLSQK"
     gene            5101..5805
                     /gene="rsmD"
                     /locus_tag="VBC52_001147"
     CDS             5101..5805
                     /gene="rsmD"
                     /locus_tag="VBC52_001147"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEO6882740.1"
                     /translation="MAIKLNNNFLIIMGTSFLLLLAYHIKKEGKEVLSSLFVISNFFD
                     NSTLFFVIIVVMRVVAGTFGGRPLKTLDGKTTRPTTDKVKGAIFNMIGPFFEGGRVLD
                     LFSGSGSLAIEAISRGMDQAVLVEKDRRAQVVIQENIAMTKSPEQFQLLKMEANRALE
                     QLTGQFDLVLLDPPYAKEEIVKQIQIMDSKGLLGDDIMIVCETDKSVDLPEEIASFGI
                     WKQKIYGISKVTVYVR"
     gene            5817..6107
                     /locus_tag="VBC52_001148"
     CDS             5817..6107
                     /locus_tag="VBC52_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882741.1"
                     /translation="MDYKLVMIILILVILIYLLLRLHYLEELLKHYHFKNDEGVCSLS
                     RTKNPLYLLMAMITFLPNLINWIANGFEAIDIVRLIPVVVFLWLYYCGDDDE"
     gene            6104..6589
                     /gene="coaD"
                     /locus_tag="VBC52_001149"
     CDS             6104..6589
                     /gene="coaD"
                     /locus_tag="VBC52_001149"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEO6882742.1"
                     /translation="MTKKALFTGSFDPVTNGHLDIIERASYLFDHVYIGLFYNLEKQG
                     YFSIECRKKMLEEAIRQFKNVSVLVAQDRLAVDLAREVGAKYFVRGLRNSQDFDYEAN
                     LEFFNKQLADDIETVYLSTSPSLSPISSSRIRELIYFKASVKPFVPKSVVREVEKMSE
                     E"
     gene            6579..7652
                     /locus_tag="VBC52_001150"
     CDS             6579..7652
                     /locus_tag="VBC52_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEO6882743.1"
                     /translation="MKNKDPKRKHKSLLGLLKWWIIGFAFLLLVLASLVVRLPYYLEM
                     PGGAYDIRSVLKVNKKADKAKGSYNFVAVSVSQATPAQVLYAWLTPFTELSSKEETTG
                     GFSNDDYLRINQFYMETSQNESVYQALKLANKQVSLTYKGVYVLNLAKNSTFKDRLHL
                     ADTVTGVNGKSFKNSSQLIKYVAALHLGDKVKVQYTSQGKKKESVGKVIKLSNGKNGI
                     GIGLTDHTEVSSDVPVDFNTEGVGGPSAGLMFTLAIYDQLVKEDLRKGRKIAGTGTIE
                     QNGHVGDIGGAGLKVVSAAKKGMDIFFVPNNPIDKNAKKGKTKVQTNYQEAKAAAKRL
                     GTKMKIVPVQNVQQAIDYLKKTK"
     gene            7727..9061
                     /locus_tag="VBC52_001151"
     CDS             7727..9061
                     /locus_tag="VBC52_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO6882744.1"
                     /translation="MTELIRILHLNDLHSHFENFPKVKRFFHDNQAQPIETISLDLGD
                     NIDKSHPLTEASSGKANVQLMNELGIELATIGNNEGVGLSKKDLDQVYKDSDFTVIVG
                     NLKDNIIEPSWAKPYIIYETQQGTKLAFLAYTFPYYKTYEPNGWTIEDPIDCLKCHLQ
                     INEIKEANCRILMSHLGIRFDTRIAQEFSEIDLIIGAHTHHLFEEGELITGTYLAAAG
                     KYGRFVGSIDITFDNHTLKDILISTYDTKQLTGYPSDSDWAKEIESEGKEQLRKESLI
                     SFPAPLSLEESCQLVMDAMLFYAGADVAIINSGLIVQPFEKDFSRKNLHESLPHKMRL
                     AKLTVSSQELLEIYETIYQQGQFLAQQKIHGMGFRGKYFGEVLHSGFDYKNGKIVYNE
                     KDIDAKEEVILVIVDQYYFASYFECLKTKKVDLLFPELLRDVVADYLINMTL"
     gene            9130..9708
                     /locus_tag="VBC52_001152"
     CDS             9130..9708
                     /locus_tag="VBC52_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEO6882745.1"
                     /translation="MRKEVTPEMLNYNKYPGPQFIHFENIVKSDDIEFQLVINEKSAF
                     DVTVFGQRFSEILLKYDFIVGDWGNEQLRLRGFYKDASTIRKNSRISRLEDYIKEYCN
                     FGCAYFVLENPNPRDIKFDDERPHKRRKSRSKSQSSKSQTRNNRSQSNANAHFTSKKR
                     KDTKRRQERHIKEEQDKEMTSAKQHFVIRKKK"
     gene            9759..10865
                     /gene="rlmN"
                     /locus_tag="VBC52_001153"
     CDS             9759..10865
                     /gene="rlmN"
                     /locus_tag="VBC52_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEO6882746.1"
                     /translation="MSKKTDTPDYKPSIYSLTRDELIAWAIEHGEKKFRASQIWDWLY
                     KKRVQSFDEMTNISKDFIALLNENFVVNPLKQRIVQESADGTVKYLFELPDGMLIETV
                     LMRQHYGLSVCVTTQVGCNIGCTFCASGLIKKQRDLNNGEITAQIMLVQKYFDERGQG
                     ERVSHIVVMGIGEPFDNYTNVLKFLRTVNDDNGLAIGARHITVSTSGLAHKIREFANE
                     GVQVNLAVSLHAPNNELRSSIMRINRSFPLEKLFAAIEYYIETTNRRVTFEYIMLNGV
                     NDTPENAQELADLTKKIRKLSYVNLIPYNPVSEHDQYSRSPKERVEAFYDVLKKNGVN
                     CVVRQEHGTDIDAACGQLRSNTMKRDRQKAKVGQ"
     gene            10865..11380
                     /locus_tag="VBC52_001154"
     CDS             10865..11380
                     /locus_tag="VBC52_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEO6882747.1"
                     /translation="MLKRLLTEDGELTKISRRFVWMLVIIYCLIIVRMCFGPQIMIEG
                     VSTPNVQRFGRIVALLVPFNSFRSLDQLTSFKEIFWVIGQNVVNILLLFPLIIGLLSL
                     KPSLRKYKSVILLAFLMSLFIECTQVVLDILIDANRVFEIDDLWTNTLGGPFALWTYR
                     NIKGWLLTIRK"
     gene            11576..13321
                     /locus_tag="VBC52_001155"
     CDS             11576..13321
                     /locus_tag="VBC52_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6882748.1"
                     /translation="MSIIKNLWWFFKEEKKRYLIGILSLSLVAVLNLIPPKIMGSVID
                     AITTGKLTRPQLLWNLLGLVLSALAMYGLRYIWRMYILGTSYKLGQVVRYRLFEHFTK
                     MSPSFYQKYRTGDLMAHATNDINSLTRLAGGGVMSAVDASITALVTLITMFFTISWQM
                     TLIAVIPLPLMALATSKLGRKTHETFKESQAAFSELNNKVQESVSGVKVTKSFGYQEQ
                     EIASFQEVNQMTFVKNMRTMTYDVMFDPLVLLFIGASYVLTLAMGAFMISKGQVTVGD
                     LVTFVTYLDMLVWPLMAIGFLFNMVQRGSVSYNRINSLLEQESDITDPLNPIKPVVNG
                     TLRYDIDFFRYDNEETLADIHFTLEKGQTLGLVGQTGSGKTSLIKLLLREHDVTQGKI
                     TLNKHDIRDYRLSDLRQLIGYVPQDQFLFATSILENVRFGNPTLSINAVKKATKLAHV
                     YDDIEQMPAGFETLIGEKGVSLSGGQKQRIAMSRAMILDPDILILDDSLSAVDAKTEH
                     AIIENLKTNRQGKSTIISAHRLSAVVHADLILVMQDGRVIERGQHQELLNKGGWYAET
                     YASQQLEMEEAFDEV"
     gene            13311..15050
                     /locus_tag="VBC52_001156"
     CDS             13311..15050
                     /locus_tag="VBC52_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6882749.1"
                     /translation="MKSNQWQVFKRLISYLRPYKWFTVLALSLLLLTTVVKNIIPLIA
                     SHFIDHYLTNVNQTAVLILVGYYSMYVLQTLIQYFGNLFFARVSYSIVRDIRRDAFAN
                     MERLGMSYFDRTPAGSIVSRITNDTEAISDMFSGILSSFISAIFIFTVTLYTMLMLDI
                     KLTGLVALLLPVIFILVNVYRKKSVTVIAKTRSLLSDINSKLSESIEGIRIVQAFGQE
                     ERLKTEFEEINKEHVVYANRSMALDSLFLRPAMSLLKLLAYAVLMSYFGFTGVKGGLT
                     AGLMYAFIQYVNRLFDPLIEVTQNFSTLQTSMVSAGRVFDLIDETGFEPSQKNTEAFV
                     REGNIEFKNVSFSYDGKKQILDNVSFSVKKGETIAFVGATGSGKSSIINVFMRFYEFQ
                     SGQVLLDGKDIRDYSQEQLRKNIGLVLQDPFLYHGTIKSNIKMYQDITDQEVQDAAEF
                     VDADQFIQKLPDKYDSAVSERGSSFSTGQRQLLAFARTVASKPKILILDEATANIDSE
                     TEQIVQDSLAKMRQGRTTIAIAHRLSTIQDANCIYVLDRGKIIESGNHESLLDQKGTY
                     YRMYQLQAGMMEV"
     gene            15178..15744
                     /locus_tag="VBC52_001157"
     CDS             15178..15744
                     /locus_tag="VBC52_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEO6882750.1"
                     /translation="MLLLVDNYDSFTYNLKQYLSVYKEIFVIKNDDPNLFLLAESAEA
                     IVLSPGPGHPKDAGKMLELINQFIGKKPILGICLGHQALAECLGGRLNLANHVMHGKQ
                     SWVTINDHTSLFKGIDSPTQVMRYHSLVVTDLPENIAVIARSNEDNEIMAFHCPSLKV
                     YAMQFHPESIGSIDGMKMIENFLTLIND"
     gene            complement(15811..16350)
                     /locus_tag="VBC52_001158"
     CDS             complement(15811..16350)
                     /locus_tag="VBC52_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEO6882751.1"
                     /translation="MNTRTTTYIALMVALLIVLGFIPGIPLGFIPVPIVLQNLGVMLA
                     GALLGSRKGFLAVAIFLLLVAIGAPFLPGGRSGLVTLFGPTAGYLLTYPFAAFFIGLG
                     LEKVKTTKLWVQFLIIWIFGVLLIDICGSIVLSFQTSLPLTKSLFSNLIFIPGDTLKA
                     SICLIIYRKFANRLTHLYN"
     gene            complement(16351..17343)
                     /gene="bioB"
                     /locus_tag="VBC52_001159"
     CDS             complement(16351..17343)
                     /gene="bioB"
                     /locus_tag="VBC52_001159"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEO6882752.1"
                     /translation="MSFQTNYIHLADEILSGKTSISYEQALEILNSDENWWEIYAAAL
                     YLKNQVSRNNIRLNVLLSAKQGLCAENCGYCSQSKESTADIDKFGLLPQNVILKQAIV
                     AHQNGASVFCIAMSGTKPSKREIEQLCQVIPEIKKSLPLEICLTAGFLDREQLHQLKQ
                     AGIDRINHNLNTPEENYPNIATTHSFKDRCDTLERIHNEDIDVCSGFICGMGESDEGL
                     ITLAFRLKELNPYSIPVNFLLAVEGTPLGKYNYLTPIKCLKIMAMLRFVFPFKELRLS
                     AGREVHFENFESLVTLLVDSTFLGNYLTEGGRNQHTDIEFLEKLQLNHTKKELI"
     gene            17468..17983
                     /locus_tag="VBC52_001160"
     CDS             17468..17983
                     /locus_tag="VBC52_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882753.1"
                     /translation="MKKLFKKAIAMCRDLPLNFVIVELSLYQREDGSSDNIKWISKRI
                     LEVTVTDQQWIYHLRMLNLPKLKPMSLASSDCPAREEKVSLISIEECQKFTSLVRDKN
                     PFHQGEAAIVPAALLLEKIMEVRKGKIKEIHLTFLSPVRVNQEFSVQVVDNTVIIFQH
                     QILCVRGTYEE"
     gene            17973..19088
                     /locus_tag="VBC52_001161"
     CDS             17973..19088
                     /locus_tag="VBC52_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEO6882754.1"
                     /translation="MKNRDVYIGFGLRTPIGIKGKQFKHYRPELLGAHLLNQIKKIES
                     ESNIDSIICGNTVGTGGNIGRLMTLFSDYESYIPVQTIDMQCASSSSALFFGYLKIST
                     GINEKVLVGGIESSSLQPMRRYAKEDNRNGEYTVAQFSPDSYAETVMLEGAQRVCQKY
                     GFRREMLDKLAFLSHKRALTAKQGGYLEEVILPMEGMRDQGVRKLKETFFQKLPRLME
                     NSPLLTIGNVCLMHDAAAFLTLQSQKTEFRIVHIVEVAGDPKLSPELVHTATEKLLTE
                     THTKISDYDAIEWNEPFAAIDALFNHYYPEEREKFNIFGGALAYGHPYACSGIINILH
                     LMQALKYKNKPMGLTAIAGAGGVGMAISIEYLGVKNA"
     gene            19081..20310
                     /locus_tag="VBC52_001162"
     CDS             19081..20310
                     /locus_tag="VBC52_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEO6882755.1"
                     /translation="MLESLKTIVKTNSDKKLFDGDLQVSYGEFYNLVRQDMASQDNRK
                     HVISTHSLLNQLVRFVSKLCKKALPIICKPNLTHNEISRLEKEVQYAPQLADFGVLSS
                     GTTADAKLLWRSFTSWSDFFSIQNAYFSVTSNSKLFIQGDFSFTGNLNLALSLLLLGG
                     TLVVTQKNSVKYWQTLWEKTGVTHLYLLPSYLKLVEQYSKETALDNKTIITSSQYVSD
                     SLLEGLYRKHPKVSVKIFYGASELNYVSWYDGRDIRDKPQYVGEIVPNVAVRIKEGRI
                     FVKTPYSICGLSSEYCAGDYGELIDGKLYLFGRGGDWCNQSGIKLYLPRLIEKIKTCP
                     YIKDAVAFTKESQSHGQESHCCIVLIENQMQQECLKWLSEHFEKKYGFKHYHIVSKIP
                     LMPSGKIDYQQLKRQLA"
     gene            20423..21055
                     /gene="nth"
                     /locus_tag="VBC52_001163"
     CDS             20423..21055
                     /gene="nth"
                     /locus_tag="VBC52_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEO6882756.1"
                     /translation="MLSKAKSRYIIREIIKLFPDAKPSLDFTNVFELLVAVMLSAQTT
                     DAAVNKVTPALFERFPNPLVLAQADPKEIEPYISKIGLYRNKARFLNQCAKQLIEHFD
                     GKVPRTRQELESLAGVGRKTANVVMSVGFGIPAFAVDTHVTRICKHHQICKQSASPLE
                     IEKRVMEVLPPEEWLAAHQSMIYFGRAICHPKNPKCDQYPQLYHFPDNLK"
     gene            complement(21056..>21326)
                     /locus_tag="VBC52_001164"
     CDS             complement(21056..>21326)
                     /locus_tag="VBC52_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001884316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEO6882757.1"
                     /translation="TPITKVSIVLCLFGILAEVVDINIGSGDFLYLATIGLSLPLHQM
                     LFIIQIGAWLGIIYCLVMRKMKKTIAFLPFLSIAYIIVTSYSLLF"
BASE COUNT         6899 a         3295 c         4258 g         6874 t
ORIGIN      
        1 ttcgtttgtg gttaaaaaag gttgtggatt gccgtctaca acctttttca tttaatagta
       61 tacactataa cttaaataaa tcaatcaata tattgtgtct gagttgtttt taaaacacaa
      121 tatattgatt ttggaggata tacttaaatt tagaaaatta ttaaagtatt tgatttatga
      181 tgctgtagtt gaaaatttgt tatgttaaaa agcttatttt ttctcactct ctttttccta
      241 tcacatatgc tataatatgc ctaaatattt atgtcaaagg agtttttatg tcaaaggtaa
      301 ggtatggtgt tgtgtcaacg gcaaaggtgg cgccccgttt tattgaagga gtccgcttag
      361 caggaaatgg tgaagttgtg gctgtatcga gtcgaactct tgagtctgcg caagcttttg
      421 ccaacaaata tcatctccca aaggcttacg ataagttaga ggatatgtta gcagatgagt
      481 caattgatgt gatttatgtg gcaacaatta atcaagacca ttataaagtt gctaaagcag
      541 ctctgttagc gggaaagcat gtgttggtag agaagccttt cacattgact tacgaccaag
      601 cgaatgaatt attcgctttg gcagaaagtt gtaatctgtt tttgatggaa gcacaaaaat
      661 cggtatttat tccgatgaca caagtgatta aaaaattact tgcctctggt gaaataggag
      721 aagtgatctc catatcatct acaacagcct acccaaacat tgaccatgta acatggttta
      781 gagaacttga gttaggtgga ggaacagtgc attttatggc gccctatgcc ctgtcttatt
      841 tacaatatct atttgatgcg actataactc atgctagtgg gactgcgact tttccaaaag
      901 gtcaaagcga tagtcaatcc aaactactct tgcaattatc aaatggggtc ctcgtagata
      961 tcttcttgac aacacgtcta aatttaccgc atgaaatgat tatttacgga actgaaggtc
     1021 gtctaatcat tccacatttt tggaaaacca ctcatgcaaa attggtgaga aatgatacta
     1081 gtgcgcgaac gattcaagtt gacatggtta gtgattttga gaaagaagct tatcatgtta
     1141 gtcaaatgat tttagaaggg caaagggtaa gtcatatcat gacgcctcag ttgacgctgt
     1201 caggtgttaa gattatagaa gacttatacc gttcatgggg gaaatgatgc tggctatcta
     1261 gcatattaat aaggagtact agatgttatt ttagtactcc ttttttgttt tgctctcata
     1321 aggttaatta gatttaaaag ggttttctac ttgttttttc tcgatttttg tttcataatg
     1381 tagacaagtt tagagtgagg tgcttctatg ttttcgagcg attttggatt tgagataatt
     1441 aatgttgacg atctatcctt acaagaggag aaatttttac cttcagaatt gctggcttat
     1501 gcacgtgatg aaaatgagtc ttctttcgta cgtgatattg aggagcattt gacacttgtt
     1561 taccagcttc tggatacgca aggtcacgtt gatgatgttc gtcatgtgcc gagagtgata
     1621 ccagtgactc tatttttgaa agaagatggc ttgtttgtat tagctaatca taaaaatatt
     1681 aaccttgtaa aaaaagcact taatcgtgtt gaaaaggtag attctccgaa acacctctta
     1741 ttatcattag tgactgcttt ctcaaagcaa tattttgatg ttttagatac tatttctgaa
     1801 gaaagagata aactaatcaa tgatttgcgt aaaagaccaa acaaatcgaa cttggcaagg
     1861 ctggcaaatt tacaaagtgg aacagtacac ttaatgatgg gaactaagca aaattttgaa
     1921 atgctgaccg atttacaaaa cattgagcaa gataaagaga acactagaaa tgaaaagatg
     1981 caacttcaag atgctattat tgaagcaaga caattatcta acatgtgttc cttaaattca
     2041 caggttttcc aagaactttc ttcttcttat aataatgtcc tcagcaataa ccttaacgac
     2101 aatgtaacta ctttaacaat tatttccatt ggtatttcca ttattgctat ggttaccagt
     2161 ttttatggaa tgaatgttaa gttgcctttt gatagtgttg atgctgtctg ggtattgatt
     2221 attttaatta cgactatcat tactattatg ctctctattg ttatgtacat ttatgtccat
     2281 aaagaggata atcatcccaa acataaatag agaattaata agagggtact atcaattagt
     2341 ttagttttaa gcatggcaac atgctttttt tgtacaaaga aactagaatt catctttgac
     2401 tttgagcttg atttctagct cttaaaacga cataatagag gataatgtac agtgaataga
     2461 gaggttacta tgtcaataga atcagatttt ttccggaaaa aacgttttat tttttcatcg
     2521 cttgaggagt ttggatttat caaatctgac caagagtata tctattgtca aacatttatg
     2581 gataatgact ttaaagctat tatcacgatt tcattggatg gaaagatagc tggtaaggtt
     2641 attgatttgg cacttgaaga agaatattta ccgcttcgag cagcaaatta taatggttcg
     2701 tttgttggcg aagtacgatc ggcttatatg gctatattag gtgatatttc agattcttgt
     2761 tgtaaggact tattatttac caaagatcaa agtaatcgtt tagcggagaa gatagctaaa
     2821 acttttgaag attcagttga ttatccattt gcgaagcacc cacaatatgc ttcctatcgt
     2881 gtatcaggta aatggtatgc tctgcttttt ccattgaaga tgggaaaact agaaaatgtt
     2941 ccagctcaac tgtcagaaga agaggttgaa gttttgaata ttaaagtcaa tcctcaagat
     3001 atggaaatac tcttacaaaa agaaggtgtt tatccttctt atcatatgtc taaaaagact
     3061 tgggtatcta tcgttttaga caatactttg tctgatatag aaatttttaa attggtatca
     3121 gatagtcgta agcttgtttc tcccaataaa aaatcaaatt ctgagccaga gttttggatt
     3181 attcctgcta atcctaaatt ttacgatatt gataaggagt ttgcagagca tcaattgatt
     3241 gattggactc aaaaaggaaa gattgcggtg ggagattatg tgtttattta tattacagca
     3301 cctattcgtg ctttgcgcta tgtctgtcaa gtaatagaac gtgaccttaa aacaagtact
     3361 tacttaggag caagagatgt caaagcttat atgcgactca aactgataag gcaatttgaa
     3421 gattctacct atcctgtggc atttttagca gaacacggcg ttaagactgt gagaggacca
     3481 cggcgaatga ccaaggaatt gcgacaagtc atggcaaaaa caattctaga gtaatgactt
     3541 tcagaaaaaa acttgtaaaa aagtcagaat aattctacaa ttcctcttcc attttgttac
     3601 ttgaaggagt ataatagctc taacatttta taatttaagg aggaacatat gaagaaaagc
     3661 tttattcatc agcaacagga gatttctttt gtcaaaaata cttttacaca atatttgatt
     3721 gataagttag aaatcgtcga agtgcaagga cctattctta gtcaggtcgg ggatggtatg
     3781 caagataatt tatctggtat tgaacatcct gtatcggtga aagtccttaa tatacctgaa
     3841 gctgaatttg aagtcgttca ctcattggct aaatggaaac gtcatacttt ggcaagattt
     3901 gggtttaatg agggagaagg tcttttcgtt catatgaaag ctcttcgtcc agatgaagat
     3961 tctcttgatc caacacactc agtttacgta gatcagtggg actgggagaa ggtcattcct
     4021 gatggtagac gtaatttgga ctacctaaaa gaaactgtgg aaaaaatcta caaagctatc
     4081 cgcctaacag agcttgccgt ggaagctcgt tttgatattg aatccatttt accaaagcgt
     4141 attaccttta ttcatacaga agaattggta gaaaaatacc cagacttaag ccctaaagag
     4201 cgtgaaaatg ccatcgctaa ggagtatggc gcagtctttt tgattggtat tggtggagag
     4261 ttagcagatg gtaaaccaca tgatggtcgt gcaccggact atgatgactg gacaacacca
     4321 tcagaaaatg gttttaaagg tctcaatggt gatattttag tatggaacga acagcttgga
     4381 actgcctttg aactatcatc aatgggaatt cgagttgatg aagatgctct taaacgtcaa
     4441 gttgttctca ccggtgatga aggtcgttta gagtttgagt ggcataagac gctattacgt
     4501 ggtttcttcc ctttgactat tggtggggga attggtcaat cacgattagc tatgttcttg
     4561 cttcgtaaaa aacatattgg agaagtacag tcaagtgtct ggccaaaaga agtcagagac
     4621 acctttgaaa atatattata aaaaatcagc caagcagttt tgtttggctg attttaacaa
     4681 taagtttatt tttggcttaa gaaagtttgg atttgttgag gtgtagcttg gcttccttga
     4741 acaagatatt tagtcatttt tcctcgttga taatgtgcta aatttggaac agtggtaata
     4801 tgatgctttt tgaaaaaact cttcaatcgt ttccctttgt atttttgact atcaaggtag
     4861 taaatttttg aatgaagtgt ggctttagat tttaataatt ttggtaagaa agctctacaa
     4921 tattggcaag tttgacgtcc tataaaaata gtcgcttcag attgttgact tttgcttact
     4981 aacgccttat ctgtgatggc gattaagtca ctatttgtta tcatgtgttg gtattgttgt
     5041 tgagaaatag tcatggtgtc agcgctactt aagtttgatt ttagcgttgt aaggcataat
     5101 atggctatta aacttaataa taatttttta atcattatgg gaacctcgtt tctcttactg
     5161 ctagcttatc atatcaaaaa agaaggaaaa gaggtactat cttctctgtt tgttataagc
     5221 aatttttttg ataactctac cttatttttt gttataatag tggttatgag agtagtagca
     5281 ggaacatttg gagggcgtcc tttaaaaacg cttgatggaa aaacaactcg tccaacaact
     5341 gacaaggtta aaggcgctat ttttaatatg attggtcctt tttttgaagg tggtcgtgtt
     5401 ttagaccttt tttctggcag tggtagctta gctattgagg cgatctcaag gggaatggac
     5461 caagctgtcc tagttgaaaa agacaggcgt gcgcaggtcg ttattcaaga aaatattgca
     5521 atgactaaga gtccggagca atttcaatta ttaaaaatgg aagccaaccg tgctttagaa
     5581 caattaacgg gacaatttga tttggtcttg ttggacccgc catatgctaa ggaagaaatt
     5641 gtaaagcaaa tccaaatcat ggatagcaag ggtttattag gcgatgatat catgattgtt
     5701 tgtgaaacgg acaaatcagt tgatttacct gaagaaattg cttcttttgg gatatggaaa
     5761 cagaaaattt atggcatttc taaagtaacg gtctacgttc gttaggaggc tttggtatgg
     5821 attacaagtt agtgatgatt attctcatct tagtcatatt gatttatttg ttgttgcgcc
     5881 ttcactattt agaagagttg ctaaaacatt atcacttcaa aaatgatgaa ggagtttgtt
     5941 cattatcaag aacaaaaaat ccattgtatc tattaatggc aatgattact tttttgccaa
     6001 accttatcaa ttggatagct aacggttttg aggcaataga cattgttcgc ctgattcctg
     6061 tggtagtctt tttatggttg tattattgtg gagatgacga cgaatgacaa agaaagcttt
     6121 atttacaggt tcatttgatc ctgttactaa tggacattta gatattattg agcgcgccag
     6181 ctatctattt gatcatgttt acattgggtt attttataac cttgagaagc aaggttactt
     6241 ttcgattgag tgccgtaaaa aaatgttaga agaagctatc cgtcagttta agaatgtttc
     6301 agttttagtg gcgcaagata gactagctgt tgacttagca agggaagtgg gagcaaaata
     6361 ttttgttcga ggtctgcgta atagccagga ttttgattat gaagccaatt tggagttttt
     6421 taataaacag ttggcagatg atattgagac agtttattta tcaacgtcgc catctttgag
     6481 tccgatatca tctagtcgca tccgtgaatt gatatatttt aaggcttctg tgaagccttt
     6541 tgttccaaag agtgttgtta gagaagtgga gaaaatgagt gaagaataaa gatccaaaaa
     6601 gaaaacataa aagtttacta ggtctgctta aatggtggat tataggtttt gcttttttat
     6661 tgttagtatt agcaagcctt gtggtgaggc taccttatta tttggaaatg ccaggtggag
     6721 cttatgatat ccgttcggta ttgaaagtga ataaaaaggc tgataaagct aagggttcgt
     6781 acaattttgt agcggtttct gtcagtcaag caacgccagc tcaggttctt tatgcttggt
     6841 taactccttt tacagaatta tctagtaaag aggagacaac aggtggcttt agtaatgacg
     6901 attatctcag aattaatcaa ttctacatgg agacttctca aaatgagtcc gtctatcaag
     6961 ctttaaagtt agctaataag caagtatctc ttacttataa aggagtttat gtgcttaact
     7021 tagctaaaaa ttcgacattt aaagatagat tacatttagc tgatacagtc acaggagtta
     7081 atggcaaaag tttcaaaaat tcttctcaac ttatcaaata tgttgcagct cttcatctag
     7141 gtgataaagt aaaggtacaa tacacaagtc aaggtaaaaa gaaagaatcg gttggtaaag
     7201 ttattaaact atcaaacggt aaaaatggta ttggtatcgg tttgacagac catacagaag
     7261 tatcatctga tgttcctgtg gactttaata ctgaaggtgt tggaggacca agtgcaggtt
     7321 taatgtttac tttagcgatt tacgaccaat tggttaaaga agatttacgt aaaggacgta
     7381 agattgcagg tactggtact attgagcaaa atgggcatgt tggcgatatt ggtggagcag
     7441 gtttgaaagt tgtttcagcg gctaaaaaag gaatggatat tttctttgtt cctaataatc
     7501 cgatcgataa aaatgctaaa aaaggcaaaa caaaggttca gacgaattat caagaggcaa
     7561 aagcagcagc taagcgatta ggtacgaaga tgaaaattgt accagttcaa aatgttcagc
     7621 aagctattga ttatttgaaa aaaacaaaat aaactcaaag agttcgggcg tctgctcggc
     7681 ttttttgttt tgcgacattt aactatcaaa gtgctaaaat gatagtatga cagagcttat
     7741 aagaatacta catttaaatg atctgcactc tcattttgag aattttccta aggtaaaacg
     7801 attttttcat gacaatcaag cgcagccaat agagacgatt tcacttgact tgggggataa
     7861 tattgataaa agccatcctt tgacagaagc aagctcgggc aaggctaatg tccaattgat
     7921 gaatgaactg ggtattgagc ttgctactat tggtaataat gaaggagttg gtttatccaa
     7981 aaaagacttg gaccaagtct acaaagattc agattttacg gttattgttg gtaatctaaa
     8041 agataacata atcgaaccga gttgggcaaa accttacatt atttatgaga cacagcaagg
     8101 aactaagctt gcttttttag cttatacttt tccttattat aaaacctatg aacctaatgg
     8161 gtggacgata gaagatccaa ttgactgtct taaatgccat ttacaaataa atgagataaa
     8221 agaggcaaat tgtcgtattt tgatgagtca tttaggtatt cgttttgata caaggattgc
     8281 tcaagaattt tcagaaattg accttattat aggtgcacat acacaccatt tatttgagga
     8341 aggggagcta attaccggta catatcttgc agcagctggt aaatatggtc gctttgttgg
     8401 gagtattgat ataacttttg ataatcatac actaaaagat attttaatct caacatatga
     8461 tacaaaacaa ctaactggtt atccttcgga cagtgactgg gctaaggaga ttgagtcaga
     8521 aggtaaagaa cagcttagaa aagaaagttt aatatccttt ccagcaccat tatcattaga
     8581 agaatcgtgt caactagtca tggatgcgat gctgttttac gcaggtgcag atgttgctat
     8641 tatcaattct ggcttaattg ttcagccatt tgaaaaagac ttttcgagga aaaatctgca
     8701 tgaatcatta cctcataaaa tgcgtctcgc taaattgaca gtgagtagtc aagaactttt
     8761 agagatttat gaaactattt atcaacaagg tcaattttta gcgcaacaaa agattcatgg
     8821 tatgggcttt cgtggcaagt actttggaga agttttacat tcgggatttg attacaaaaa
     8881 tggaaaaata gtgtataatg agaaagatat tgatgccaaa gaagaagtga ttcttgtaat
     8941 cgttgatcag tattattttg catcgtattt tgagtgttta aaaacaaaaa aagtagactt
     9001 gctttttcct gaattactca gagatgtggt agcagattat ttaataaata tgacactata
     9061 aatgaaaaat agataaataa agagtagaag ctagtcgttt ctctcaatga ttttagagaa
     9121 aggagtatta tgcgaaaaga agtgacacca gagatgctta actataataa gtatcctggc
     9181 ccacagttta ttcactttga aaatatcgtt aaaagtgatg atattgaatt tcaacttgtt
     9241 attaatgaaa aatcagcttt tgatgtgact gtctttggac aacgtttttc tgagatttta
     9301 ttaaaatatg attttatcgt tggcgattgg ggtaacgagc agttgaggct aagaggcttt
     9361 tacaaagatg ctagtacgat tagaaaaaat agccggattt cacgtttaga agattatatt
     9421 aaagagtatt gtaactttgg ttgtgcttat tttgtgttgg agaatccaaa tcctagagat
     9481 attaaatttg atgatgaaag acctcataag cgtcgtaagt caagatccaa atcacaatca
     9541 tcaaagtcac aaactagaaa taatcgttcc cagtcaaatg ccaatgctca ttttacaagt
     9601 aaaaagcgta aagacacaaa acgccgtcaa gaacgtcata ttaaagaaga gcaagataag
     9661 gaaatgacct ctgcaaagca gcattttgtt attcgtaaga aaaaataaga aaaggataaa
     9721 ctgtaatcgt ttattccacc atgaagaaag aaaatattat gtctaaaaaa actgatacac
     9781 ccgactataa accctctatt tatagcttaa ctcgcgatga actaatagct tgggctattg
     9841 aacatggaga aaagaaattc cgtgcttcac aaatctggga ctggctctat aaaaagcgtg
     9901 tgcaaagctt cgatgaaatg actaacattt ccaaagattt tattgcactt ttgaatgaga
     9961 attttgttgt caatcctcta aaacaacgta ttgttcaaga atctgctgat ggaactgtta
    10021 aatacctttt tgaattacct gatggcatgc tgattgagac ggttttgatg cgtcaacatt
    10081 atggtttatc agtatgtgta acgactcaag ttggttgtaa cattggttgc actttctgtg
    10141 caagtggttt aataaagaaa caacgtgatc ttaacaatgg cgaaatcact gctcaaatta
    10201 tgcttgttca aaaatatttt gatgaacgtg gacaaggtga gcgcgttagt cacattgtgg
    10261 tcatgggaat aggtgaaccc tttgataact ataccaatgt attaaaattc ttgcgtacag
    10321 ttaacgatga taatggatta gctattggtg cacgtcacat tacagtgtct acttcaggtt
    10381 tagcacataa aattcgtgaa tttgcaaatg aaggtgtaca agttaactta gcagtatctc
    10441 ttcatgctcc aaataatgaa ttacgatcaa gtatcatgcg tattaatcgt tctttccctc
    10501 ttgaaaagtt gtttgcggct attgaatatt atattgagac gactaaccgt cgtgtaacat
    10561 ttgagtatat tatgttaaat ggtgtcaacg ataccccaga aaatgctcaa gagttagcag
    10621 atttaacgaa aaagattcgc aaattatctt atgtcaattt aattccttac aatcctgtct
    10681 cagaacatga ccagtatagt cgtagtccta aagagcgtgt ggaagcattc tatgacgttt
    10741 tgaagaaaaa tggagttaat tgtgttgttc gtcaagaaca tggtacggac attgatgcag
    10801 cttgcggaca attacgttca aacacaatga aacgcgatcg tcaaaaagcg aaggtagggc
    10861 agtaatgctt aaaagattac ttactgaaga tggggaattg acaaagatta gtcgtcgttt
    10921 cgtttggatg ttagtgatta tctattgtct tattattgtc aggatgtgtt ttgggcctca
    10981 aattatgatt gagggggtat caactccgaa tgttcagcgc ttcggaagaa ttgtagctct
    11041 tttagtacca tttaattctt ttcgtagttt agatcagcta actagcttta aagagatttt
    11101 ttgggttatt ggtcaaaatg tagtgaatat tttactgctg tttcctctca ttatagggtt
    11161 actatcccta aagccaagtt tacggaaata taaaagcgtt atattacttg ctttcttgat
    11221 gtctcttttc atagagtgta ctcaagttgt tttagatatt ttaatagatg ctaatcgggt
    11281 ttttgaaatc gacgatctat ggacaaatac cttaggcggc cctttcgccc tatggactta
    11341 tcgaaatata aaaggttggc ttctaactat tagaaaatga aaataatgta aaggctatga
    11401 cattgttcaa gccttttact atttaaaaag aatcacttta gttgacgaaa gtaagcttat
    11461 agaggcataa tagagagttg ttaaatgaga attttaaaac acttttcatg tcttctgatt
    11521 gatttggtct tctgacttaa ttatgatgtt tttcaatctt tgagaaagga aaaatatgtc
    11581 tattataaaa aatttgtggt ggttttttaa ggaagaaaag aagcgctatc ttattgggat
    11641 tttgtcatta agtttggttg cggtgttaaa tcttatccct cctaaaatca tgggatcagt
    11701 tattgatgct attacaactg gaaaattaac aagaccacaa ttactctgga atttattagg
    11761 tttggttttg tcagctttag ctatgtatgg gctgcgttat atttggcgta tgtatatttt
    11821 agggacttct tacaaattgg gtcaagttgt cagataccgt ttatttgaac attttacaaa
    11881 aatgtctcct tctttttatc agaaatatcg tacaggtgat ttaatggcgc acgcgaccaa
    11941 cgacatcaat tctctaacac gtcttgcagg aggaggagtt atgtcagcag tggatgcctc
    12001 tatcacagca ttagtaacgc ttatcaccat gttctttact atttcgtggc aaatgacatt
    12061 aattgcggtt atccctttgc ccttaatggc cttagcaact agtaaattgg ggcgaaaaac
    12121 ccatgaaacc ttcaaagaat ctcaggcagc cttttcagaa ttaaataata aagtgcaaga
    12181 aagtgtctct ggcgtcaaag tgactaaatc atttggttat caagaacaag aaattgcttc
    12241 ttttcaagag gttaatcaga tgactttcgt gaagaacatg cggaccatga cttatgatgt
    12301 catgtttgat cctttagttc ttctttttat aggtgcctcc tacgtattaa cattggctat
    12361 gggagctttt atgatttcaa aaggtcaagt tactgttggt gacttggtaa catttgtgac
    12421 gtatttagat atgttggtat ggcccttgat ggcgattggt ttcttgttca atatggtaca
    12481 gcgtggtagt gtttcttata accgtattaa tagtctactt gagcaagaat cggatataac
    12541 tgatccttta aatcctatca aacctgttgt caatggaaca ttaagatatg atattgattt
    12601 ctttagatac gacaatgaag aaaccttagc cgatattcat ttcaccttag aaaaaggtca
    12661 aaccttaggt ttggtaggtc aaacgggatc agggaagaca agtcttatta agttattgct
    12721 acgtgaacat gatgtgactc aggggaaaat tactttaaat aaacatgata tacgtgacta
    12781 tcgattgtct gacttacgtc aactaatcgg ttatgttcct caagatcagt ttttatttgc
    12841 taccagtatt ttagaaaatg ttcgctttgg aaatccaact ctatctatca atgctgtcaa
    12901 aaaagcaact aaattggctc atgtttacga tgacattgaa cagatgccag caggatttga
    12961 gactctaatt ggagaaaaag gagtctcatt atctggtgga caaaaacaaa ggattgcgat
    13021 gagtcgtgcc atgattttag atccagatat tcttattttg gatgattctc tatcagcagt
    13081 ggacgctaaa acggaacatg ccattattga gaatcttaaa acgaatcgtc aagggaaatc
    13141 gactattatt tcagcacatc gtttatcagc tgttgtgcac gcagacctta tcttagttat
    13201 gcaagacggc agagtcatcg agcgaggtca gcatcaagag ttgctaaata aaggtggttg
    13261 gtatgctgaa acgtatgcct cacagcaatt agaaatggag gaagcatttg atgaagtcta
    13321 atcaatggca agtctttaag agattaatct cctatttacg cccttataaa tggtttacag
    13381 tattagctct atctctctta ttgttgacga ctgttgttaa aaatattatt cctttaattg
    13441 cttcacattt tattgatcac tatctgacaa atgttaatca aacagcagtt cttattttag
    13501 tgggatatta ttcaatgtat gtcttgcaga ccttaattca atattttggg aatctctttt
    13561 ttgcgcgtgt ttcttatagt attgttagag atattcgtag agatgctttt gctaatatgg
    13621 aaaggctagg catgtcttat tttgatagga caccggcagg atctattgtg tcacgtatta
    13681 ctaatgatac tgaagcaata tctgatatgt tttcgggtat tttatcaagt tttatctcgg
    13741 cgatatttat ttttacagtt actctgtaca ctatgttgat gctagacatt aaactaacag
    13801 gactcgtcgc tcttttgtta cctgttatct ttatattagt gaatgtctat cggaaaaaat
    13861 cagtcactgt cattgctaaa acgagaagtt tacttagtga tatcaatagt aaattatcag
    13921 aaagtattga aggaattcgc attgtacagg cttttggtca agaagagcgc ttgaagactg
    13981 aatttgagga aattaacaaa gagcatgttg tgtatgccaa tcgttctatg gctcttgata
    14041 gtctcttctt aagaccggcg atgtctcttt taaaactcct agcatatgct gttcttatgt
    14101 cttattttgg atttacagga gttaaaggag gtcttacggc aggattaatg tatgctttta
    14161 ttcagtacgt taatcgtctc tttgaccctt taattgaagt aacgcaaaat ttttcaacct
    14221 tacaaacatc aatggtatca gcagggcgtg tgtttgatct gattgatgaa acaggttttg
    14281 aaccaagcca aaaaaataca gaagcttttg tgagagaagg aaatatcgaa tttaaaaatg
    14341 tatcattctc atatgatgga aaaaaacaaa tccttgataa tgtttctttt agtgttaaaa
    14401 aaggtgaaac tattgctttt gtaggagcga caggttctgg aaaatcatct attattaatg
    14461 tctttatgag gttttacgaa tttcaatctg gacaagtttt attagatggg aaggatatta
    14521 gggattactc acaagaacaa ttgcgaaaga atattggact cgtcttacaa gacccctttt
    14581 tatatcatgg cactattaaa tctaatatca aaatgtacca agacatcacc gatcaagagg
    14641 tacaggacgc agcagagttt gttgatgctg atcaatttat tcagaagttg ccagataagt
    14701 acgattcagc tgtttcagaa cgtgggtcaa gtttttcaac tggacaacgc caactattag
    14761 cgtttgccag aactgtggct agtaaaccta aaattttaat cttagatgag gcgacagcaa
    14821 atattgattc ggaaacagag cagattgtac aagattcgtt agcaaaaatg cgtcaaggtc
    14881 gaacaacaat tgccattgct caccgcttat caaccattca agatgctaat tgtatctatg
    14941 ttcttgatag aggaaagata attgaaagtg ggaatcacga gtctttatta gaccaaaaag
    15001 ggacttatta tcgtatgtac caattacaag ccggcatgat ggaggtctag aaaatttagg
    15061 ctattcaaaa ggcataatct gtgaggctta gaggttggaa tagttattcc tctaagcctt
    15121 ttttatattt tacaattagt gtataatgag gtcaaagtac aaggaaaggt ctataaaatg
    15181 ttactattgg tagataatta cgattctttt acctataatt taaagcaata tttaagtgtc
    15241 tataaagaga tattcgtcat aaaaaatgat gatcctaacc tttttttatt agctgagagc
    15301 gcagaagcca tcgtcttatc acctggacca ggacacccaa aggatgctgg gaaaatgtta
    15361 gaattaatca atcagtttat aggaaagaaa cctattctag gtatctgttt ggggcatcaa
    15421 gcgcttgctg agtgcttggg tgggcgatta aatttagcaa atcacgttat gcatggtaaa
    15481 caaagttggg taacaataaa cgaccatact agtttgttta agggaattga tagtccaacc
    15541 caagttatgc gttatcactc attggtagtg actgatttac cagagaatat tgctgttatt
    15601 gcacgatcaa atgaggataa tgaaatcatg gcattccatt gccccagctt aaaggtatat
    15661 gcgatgcagt ttcatcctga gtcaatcggg agtattgatg gaatgaaaat gattgaaaac
    15721 tttttgacgt taattaatga ctaaaatgtg ttagtgtagg ataatgactc tctataaata
    15781 aaagacagta cctaaggtac tgtcttttat ttagttgtat agatgagtta atctgtttgc
    15841 aaatttacga taaataatca agcaaatgga tgcttttaag gtatccccag gaataaaaat
    15901 aaggttagaa aatagtgatt tagttaaggg gagagaagtt tgaaaggaaa gaacgatgct
    15961 accacaaata tcaatcaata gtacgccaaa tatccaaata ataaggaatt gaacccagag
    16021 ttttgttgtt ttcactttct caagccctag gccgataaaa aaagctgcaa atggataagt
    16081 cagcagatag cctgctgttg gcccaaacag cgttacgaga cctgaccgtc ctcctggtaa
    16141 aaatggtgca ccaatagcaa ctaagagcaa gaaaatagcc acagctagga aacctttcct
    16201 actaccaagc agagcacctg ccagcataac accaagattt tgtaagacaa ttgggactgg
    16261 aatgaaacct aatgggatcc ctgggataaa acctaaaaca atgagtagtg caaccatgag
    16321 tgcaatatag gtagttgttc ttgtattcat ttaaattaat tcctttttag tatgatttag
    16381 ttgtaatttt tccaagaatt caatatcggt atgttgattg cgaccgccct ctgttaggta
    16441 atttcccaaa aaagttgagt caacaagtaa ggtgactaat gattcaaaat tctcaaaatg
    16501 gacctctcgt ccagcgctta atcttaattc cttgaaagga aaaacaaaac gcaacatggc
    16561 cataattttt aagcatttaa tgggagtcaa atagttatat tttccaagag gtgttccttc
    16621 aacagcaagt aaaaaattga cagggataga ataggggttc agctctttta gtctgaaagc
    16681 taatgtgatg agcccctcat cactctctcc cataccacaa atgaatccag aacaaacatc
    16741 aatgtcttca ttgtgaattc tttctaaagt atcacaacga tctttgaagc tatgcgtcgt
    16801 tgcaatgttg gggtagtttt cttcgggggt gttgagatta tgattgatac ggtcaattcc
    16861 tgcttgtttt agctgatgaa gttgttctct atccaaaaaa ccagcggtaa ggcatatttc
    16921 tagagggagg ctttttttga tttcagggat gacttgacat aactgctcaa tttctctctt
    16981 gctaggctta gttccactca tcgcaataca aaagacgcta gcaccatttt gatgagcgac
    17041 aatagcctgt tttagaatga cattttgagg aagcagacca aatttatcaa tgtcagcagt
    17101 gctttcttta gattgagaac agtaaccaca attttctgca caaagtcctt gcttagcact
    17161 taataaaacg ttaaggcgaa tattatttcg gctaacttga ttcttaaggt ataaggcagc
    17221 agcgtagatt tcccaccagt tctcatcgct atttagaatt tccagcgctt gttcataaga
    17281 aatgctagtc tttccagata atatttcgtc agccaaatgg atataatttg tttgaaatga
    17341 catgataatc tcctttaacg aaattaataa tagcataaaa aaactatatt gtaaacttag
    17401 tctatataat aagttaacaa tataaaaaat tatgatatac tggtcttata ttttgacggg
    17461 agataagatg aaaaaactat ttaaaaaagc gatagctatg tgccgagatt tgccgcttaa
    17521 ttttgtaatt gttgaactta gtttgtatca aagagaggat ggctcatctg ataatattaa
    17581 atggatttct aagcgaatcc ttgaggtaac ggtaactgat cagcaatgga tttaccattt
    17641 gaggatgctt aatttaccaa aacttaagcc aatgtcacta gcatccagtg actgtcctgc
    17701 aagggaagag aaggttagtc ttatttcaat agaagagtgt caaaaattca ctagcttagt
    17761 gcgagataaa aatccttttc accaagggga agcagctatt gtgcctgcgg ctctattatt
    17821 ggaaaaaata atggaagtta gaaaaggaaa aataaaggag attcatttga cttttttatc
    17881 tccagtaagg gtgaatcaag aattctcagt gcaagtagtg gataacacag tgattatttt
    17941 tcagcatcaa atattatgtg tgaggggaac atatgaagaa tagagatgtt tatattggtt
    18001 ttgggctacg aacacctatc ggtataaaag ggaagcaatt taaacattac cgtccagaac
    18061 ttttaggagc acacctttta aatcaaataa aaaaaataga atcagaatct aacattgata
    18121 gtattatttg tgggaacaca gttggtactg ggggcaatat tggtcgtttg atgactcttt
    18181 tttctgatta tgaatcctat attccagtac aaacgattga tatgcagtgt gcttcatcaa
    18241 gttctgcctt gttttttggt tatctaaaaa tcagtaccgg tattaatgaa aaagttcttg
    18301 ttggggggat tgaaagtagt tctcttcaac ctatgagacg ttatgctaaa gaagataatc
    18361 gtaacggaga atatacagtt gctcagtttt ctcctgactc ttatgctgaa actgtaatgt
    18421 tagaaggggc ccagagagtc tgtcaaaaat atggttttag aagagaaatg ttagataaat
    18481 tggcattctt gagccataaa cgcgccttaa cagctaaaca aggtggctat ttagaagagg
    18541 taatcttacc aatggaaggg atgcgagatc aaggcgttag aaaactaaaa gaaacatttt
    18601 ttcaaaaatt accaagattg atggaaaatt cacctttgct cactattgga aatgtttgtt
    18661 taatgcacga tgctgctgca tttctaacgc ttcagagtca gaaaacagaa ttccggattg
    18721 ttcacattgt agaagtagca ggagatccca agcttagtcc agaattggtt cacacggcta
    18781 cggaaaaact attaacagaa actcatacta aaatatcgga ttatgatgca attgaatgga
    18841 atgaaccatt tgcagcgatt gatgctctat ttaatcatta ttatcctgaa gagagagaaa
    18901 aattcaatat ttttggaggg gcattagctt acggacaccc ttatgcctgc tcaggaatta
    18961 ttaatatcct tcatcttatg caggcattaa aatataaaaa taaacctatg ggtctaactg
    19021 ccattgcagg ggcaggagga gtcggaatgg ccatatcaat agagtactta ggagttaaga
    19081 atgcttgaat cattgaaaac gatagttaaa accaatagcg ataaaaagtt atttgatggt
    19141 gatttacagg tcagttatgg agaattttat aacttagtaa gacaagatat ggcgtcacaa
    19201 gacaatagga aacatgtgat tagcacacac tctctgctta accaattagt aagatttgtt
    19261 tcaaagttat gtaaaaaagc tcttcctatt atttgtaaac ctaaccttac tcataatgaa
    19321 ataagtcgat tagaaaaaga agtgcagtat gctcctcaac tagctgattt tggtgttctt
    19381 tcttctggga ctacagctga tgctaaacta ttgtggagga gcttcacttc gtggtctgat
    19441 ttttttagca ttcagaatgc ttatttttcg gtcacttcca atagcaaact gtttattcaa
    19501 ggtgattttt catttactgg taacttaaat ttagcgctat cccttttatt actgggggga
    19561 acacttgttg tcacacagaa aaattctgta aaatactggc agactttatg ggaaaaaact
    19621 ggtgtaacgc atctatatct gctccctagt tatctcaagt tagtggaaca atatagcaag
    19681 gagactgcgt tagacaataa aactattatt acctcttcgc aatatgtctc tgactcatta
    19741 ctggaaggtt tatatagaaa gcatcctaaa gtgtcagtga aaatcttcta tggtgctagt
    19801 gagctcaatt atgtgtcatg gtacgatggt cgtgatatcc gagataagcc acagtatgtt
    19861 ggagaaatcg ttccaaatgt agctgttagg attaaagagg gaagaatttt tgtaaaaaca
    19921 ccgtatagta tctgtggcct ttcaagtgaa tattgtgctg gtgattatgg tgaactgata
    19981 gatggcaaat tatatctatt tggccgtggt ggtgattggt gcaatcaatc gggcattaag
    20041 ctctatttac ctagattaat cgaaaaaata aaaacatgcc cttatatcaa ggatgcagta
    20101 gcatttacaa aagagagtca aagtcatgga caagagtcac attgctgtat tgttttaatt
    20161 gaaaatcaga tgcaacaaga gtgtttgaag tggttatcgg aacattttga aaagaagtat
    20221 gggtttaagc attatcatat tgtatctaaa attcctttga tgccatctgg aaaaattgat
    20281 tatcaacaac tcaaaagaca gttagcatga gcttttacta aaaaaataaa atagggttaa
    20341 tggattgcaa acctcagttc tgatattctt tattttgaaa gatatcgtga tataatgata
    20401 aaaaagaaag ataaggaata aaatgttatc aaaagcaaaa tcacgatata ttatccgtga
    20461 gattattaag ttatttccag atgctaaacc tagcttagac tttactaatg tctttgagct
    20521 tttggtagct gtaatgctat cagctcaaac aactgatgcg gctgttaata aagttacacc
    20581 cgcccttttt gaacgctttc caaatccttt agttttggca caagctgatc ccaaagagat
    20641 tgaaccctat atttctaaaa ttgggcttta ccgtaataaa gctcgattct taaatcaatg
    20701 cgctaaacaa ttgatagagc attttgatgg aaaagtcccc cgaactcgtc aagagctaga
    20761 aagtttggct ggcgttggaa ggaaaacggc taatgtggtc atgagtgttg gatttggtat
    20821 tccggctttt gccgtagata ctcatgtcac tcggatttgt aaacaccacc agatttgcaa
    20881 acagtcggct tctccactgg aaattgagaa acgagtcatg gaggttttac caccagaaga
    20941 gtggctagct gctcatcaat ccatgattta ttttggtcgt gcaatttgtc atcctaaaaa
    21001 tccaaaatgc gaccagtacc cacaacttta tcactttcct gataatttaa agtgattaga
    21061 ataaaagaga gtaactggtg acgattatat aagcgatact taaaaacggt agaaaagcta
    21121 tcgttttttt catttttctc ataactaaac aataaataat tcctaaccaa gcacctattt
    21181 gaataataaa aagcatttga tgaagaggta aagataagcc aattgtggct aaataaagga
    21241 aatctccact tccgatatta atatcaacca cttcagctaa aatcccaaat aaacacaaaa
    21301 caatagaaac ttttgtaatc ggtgtg
//
LOCUS       DATRNK010000031        21189 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.031, whole genome shotgun sequence.
ACCESSION   DATRNK010000031 DATRNK010000000
VERSION     DATRNK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21189
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.031"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(42..653)
                     /locus_tag="VBC52_001165"
     CDS             complement(42..653)
                     /locus_tag="VBC52_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEO6882758.1"
                     /translation="MNMKLSKRYRFWQKVIKALGVLALIATLVLVVYLYKLGILNDSN
                     ELKDLVHKYEFWGPMIFIVAQIVQIVFPVIPGGVTTVAGFLIFGPTLGFIYNYIGIII
                     GSVILFWLVKFYGRKFVLLFMDQKTFDKYESKLETSGYEKFFIFCMASPISPADIMVM
                     ITGLSNMSIKRFVTIIMITKPISIIGYSYLWIYGGDILKNFLN"
     gene            746..1095
                     /gene="ssrA"
                     /locus_tag="VBC52_001166"
     tmRNA           746..1095
                     /gene="ssrA"
                     /locus_tag="VBC52_001166"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1421..2002
                     /locus_tag="VBC52_001167"
     CDS             1421..2002
                     /locus_tag="VBC52_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HEO6882759.1"
                     /translation="MRTDMSTGASYPKYGFSVGKYTYGYQQFFYEGVNLKEIGAFCSI
                     AQNVTITGLNHPTDHITTNPFIYYKSRGFINEDRADLIDEKKNGKVIIGNDVWIGTNV
                     TILPSVTIGNGAIIGAGSVITKDIPDYAVVAGTPAKIIKYRFSEEEITLLNASQWWNW
                     SDEAIKEHISEFSDKKEFFNTLKSISENKNHKL"
     gene            complement(2123..3226)
                     /locus_tag="VBC52_001168"
     CDS             complement(2123..3226)
                     /locus_tag="VBC52_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6882760.1"
                     /translation="MSNIITYLKNNSNLTFDELALNDVDILCLNEFGYISFEKLINTT
                     EMKSVLVCELYHEYLQTMAKSYSFMFTSQRHDLCQLMMTSKRFKNLTLSYYRAEISLE
                     FEKQFAAMVFTIPNINYHQVVFRGTDANLIGWKEDFKLTYMREISAHRSAIKYLNTIL
                     PYFDKVVLSGHSKGGNLALYAAMFTKPDLKAKIDLIWLIDSPGLQKTLLPTTEYKTTK
                     QKCIRLLPEESIVGMMLYSDIEPLIISSNARGILQHDVTTWEIQEPAILKTGTGLSLK
                     SICFEKTFQQWMAELKSQERKLFFDLLFDSFLSSGVSSLDDFNLASRAKMMKAFHSFR
                     ELDDDKKRLFNKSLKLLVTIFWGAYHDNSRETK"
     gene            complement(3226..6018)
                     /locus_tag="VBC52_001169"
                     /pseudo
     CDS             complement(3226..6018)
                     /locus_tag="VBC52_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
     gene            complement(6168..6602)
                     /gene="msrB"
                     /locus_tag="VBC52_001170"
     CDS             complement(6168..6602)
                     /gene="msrB"
                     /locus_tag="VBC52_001170"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEO6882761.1"
                     /translation="MKETQEELRQRIGHTAYQVTQNSATEHAFTGKYDDFFEEGIYVD
                     IVSGEVLFSSLDKFQSGCGWPAFSKPIENRMVTNHQDHSHGMHRIEVRSRQADSHLGH
                     VFNDGPVDAGGLRYCINSAALDFIPYDQMAKRGYGDYLSLFD"
     gene            complement(6602..7066)
                     /locus_tag="VBC52_001171"
     CDS             complement(6602..7066)
                     /locus_tag="VBC52_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6882762.1"
                     /translation="MIRRAKEKDLPDIAELLKQILMLHHEVRPDIFHTRGSKFSKEQL
                     KEMLIDESKPIFVYESDEGKVVAHLFLQLQEKRDLPRKSFKTLYIDDLCIDEEVRGQQ
                     IGQKLMDFARQYAKKHGCYNITLNVWNDNQRAVSFYEKLGFKPQQTQMEEIL"
     gene            complement(7075..7734)
                     /locus_tag="VBC52_001172"
     CDS             complement(7075..7734)
                     /locus_tag="VBC52_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001884604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6882763.1"
                     /translation="MTMVKREDVILNATENMVHHKLKNDPSGHDWFHIVRVRNLAVEL
                     AHKEGANTFICQMAALLHDIIDDKICQDSKQASYELTQWLYSQDLAIAEVEHILDILE
                     NISFKAGTGLTMKTLEGQIVQDADRLDAMGAIGIARTMAYSGSKGRLIHDPNLKPREN
                     LTLEEYRNGQDTAIMHFYEKLLKLKDLMNTKQGKMLAQKRHDFLELYLAEFYAEWNGK
                     R"
     gene            complement(7834..10467)
                     /locus_tag="VBC52_001173"
                     /pseudo
     CDS             complement(7834..10467)
                     /locus_tag="VBC52_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678288.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(10714..12546)
                     /gene="lepA"
                     /locus_tag="VBC52_001174"
     CDS             complement(10714..12546)
                     /gene="lepA"
                     /locus_tag="VBC52_001174"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEO6882764.1"
                     /translation="MNIEDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRAEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGEVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGMVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYIAASIKTVAD
                     TRVGDTITLANNPAIEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVNTTDGEMLE
                     VSNPSEFPDPTRVDSIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYEISEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDDKK"
     gene            complement(12682..13098)
                     /gene="ndk"
                     /locus_tag="VBC52_001175"
     CDS             complement(12682..13098)
                     /gene="ndk"
                     /locus_tag="VBC52_001175"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEO6882765.1"
                     /translation="MEQTFFMIKPDGVKRGFIGEVISRIERRGFSIDRLEVRYADADI
                     LKRHYAELTDRPFFPTLVDYMTSGPVIIGVISGEEVISTWRTMMGSTNPKDALPGTIR
                     GDFAQAPSPNQATCNIVHGSDSPESATREIAIWFNN"
     repeat_region   13656..14813
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=13722..13757
                     /rpt_unit_seq="gttttggaaccattcgaaacagcacagctctaaaac"
     gene            complement(14914..15579)
                     /gene="csn2"
                     /locus_tag="VBC52_001176"
     CDS             complement(14914..15579)
                     /gene="csn2"
                     /locus_tag="VBC52_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEO6882766.1"
                     /translation="MIKINFPILDEPLVLSNATILTIEDVSVYSSLVKHFYQYDVDEH
                     LKLFDDKQKSLKATELMLVTDILGYDVNSAPILKLIHGDLENQFNEKPEVKSMVEKLA
                     ATITELIAFECLENELDLEYDEITILELIKALGVKIETQSDTIFEKCFEIIQVYHYLT
                     KKNLLVFVNSGAYLTKDEVIKLCEYINLMQKSVLFLEPRRLYDLPQYVIDKDYFLIGE
                     NMV"
     gene            complement(15566..15907)
                     /gene="cas2"
                     /locus_tag="VBC52_001177"
     CDS             complement(15566..15907)
                     /gene="cas2"
                     /locus_tag="VBC52_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEO6882767.1"
                     /translation="MSYRYMRMILMFDMPTETAEERKAYRKFRKFLLSEGFIMHQFSV
                     YSKLLLNNTANNAMIGRLKVNNPKKGNITLLTVTEKQFARMVYLHGERNTSVANSDSR
                     LVFLGDSYDQD"
     gene            complement(15904..16773)
                     /gene="cas1"
                     /locus_tag="VBC52_001178"
     CDS             complement(15904..16773)
                     /gene="cas1"
                     /locus_tag="VBC52_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEO6882768.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLIFKDSYQTEMIHLSEIDILIME
                     TTDIVLSTMLIKRLVDENILVIFCDDKRLPTAMLMPYYARHDSSLQLSRQMSWIEDVK
                     ADVWTSIIAQKILNQSFYLGECSFFEKSQSIMNLYHDLEPFDPSNREGHAARIYFNTL
                     FGNDFSREQDNTINAGLDYGYSLLLSMFAREVVKCGCMTQFGLKHANQFNQFNLASDI
                     MEPFRPIVDRIIYENRQSDFVKMKRELFSMFSETYSYNGKEMYLSNIVSDYTKKVIKS
                     LNSDGNGIPEFRI"
     gene            complement(16775..20887)
                     /gene="cas9"
                     /locus_tag="VBC52_001179"
     CDS             complement(16775..20887)
                     /gene="cas9"
                     /locus_tag="VBC52_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040090.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEO6882769.1"
                     /translation="MNKPYSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIK
                     KNLIGALLFDGGNTAADRRLKRTARRRYTRRRNRILYLQEIFAEEMSKVDDSFFHRLE
                     DSFLVEEDKRGSKYPIFATLQEEKDYHEKFSTIYHLRKELADKKEKADLRLIYIALAH
                     IIKFRGHFLIEDDSFDVRNTDISKQYQDFLEIFNTTFENNDLLSQNVDVEAILTDKIS
                     KSAKKDRILAQYPNQKSTGIFAEFLKLIVGNQADFKKYFNLEDKTPLQFAKDSYDEDL
                     ENLLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLK
                     QLKQFVKASLPEKYQEIFADSSKDGYAGYIEGKTNQEAFYKYLSKLLTKQEDSENFLE
                     KIKNEDFLRKQRTFDNGSIPHQVHLTELKAIIRRQSEYYPFLKENQDRIEKILTFRIP
                     YYIGPLAREKSDFAWMTRKTDDSIRPWNFEDLVDKEKSAEAFIHRMTNNDFYLPEEKV
                     LPKHSLIYEKFTVYNELTKVRYKNEQGETYFFDSNIKQEIFDGVFKEHRKVSKKKLLD
                     FLAKEYEEFRIVDVIGLDKENKAFNASLGTYHDLEKILDKDFLDNPDNESILEDIVQT
                     LTLFEDREMIKKRLENYKDLFTESQLKKLYRRHYTGWGRLSAKLINGIRDKESQKTIL
                     DYLIDDGRSNRNFMQLINDDGLSFKSIISKAQAGSHSDNLKEVVGELAGSPAIKKGIL
                     QSLKIVDELVKVMGYEPEQIVVEMARENQTTNQGRRNSRQRYKLLDDGVKNLASDLNG
                     NILKEYPTDNQALQNERLFLYYLQNGRDMYTGEALDIDNLSQYDIDHIIPQAFIKDDS
                     IDNRVLVSSAKNRGKSDDVPSLEIVKDCKVFWKKLLDAKLMSQRKYDNLTKAERGGLT
                     SDDKARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIRKVKIVTLKSNLVSNFR
                     KEFGFYKIREVNNYHHAHDAYLNAVVAKAILTKYPQLEPEFVYGDYPKYNSYKTRKSA
                     TEKLFFYSNIMNFFKTKVTLADGTVVVKDDIEVNNDTGEIVWDKKKHFATVRKVLSYP
                     QNNIVKKTEIQTGGFSKESILAHGNSDKLIPRKTKDIYLDPKKYGGFDSPIVAYSVLV
                     VADIKKGKAQKLKTVTELLGITIMERSRFEKNPSAFLESKGYLNIRADKLIILPKYSL
                     FELENGRRRLLASAGELQKGNELALPTQFMKFLYLASRYNESKGKPEEIEKKQEFVNQ
                     HVSYFDDILQLINDFSKRVILADANLEKINKLYQDNKENISVDELANNIINLFTFTSL
                     GAPAAFKFFDKIVDRKRYTSTKEVLNSTLIHQSITGLYETRIDLGKLGED"
BASE COUNT         6732 a         4056 c         3273 g         7128 t
ORIGIN      
        1 aataaaaaga ttggagatat atctccaacc tttttttatt tttaattcaa aaaatttttc
       61 aaaatatcac cgccataaat ccacaaatag ctataaccga tgatagaaat aggtttcgtt
      121 atcataatga ttgtcacaaa gcgcttaata gacatattag atagaccggt aatcatcacc
      181 atgatgtccg ctggtgaaat gggagatgcc atacaaaaga taaaaaactt ttcatatccg
      241 gatgtttcta gcttagactc atatttatca aatgtcttct gatccataaa tagcaaaacg
      301 aattttcgtc cataaaattt gactaaccaa aaaaggatga cacttccgat gataatccca
      361 atatagttat aaataaagcc taaggtagga ccaaaaatta agaaacctgc tactgttgta
      421 acacctccag gaattactgg aaaaacaatc tgtacaattt gagccacaat aaaaatcatt
      481 ggaccccaaa attcatactt gtgtactagg tcttttaatt cattgctatc attcaaaata
      541 ccaagtttgt acaaataaac aactaagaca agagtcgcta ttaacgctaa gacacccaaa
      601 gccttaataa ccttctgcca aaagcggtaa cgttttgata atttcatatt catgctttaa
      661 tcataacaca actgttaaat ttttgctatt ttaaatagta tttgttatac tatattggta
      721 agtttttatg ctttattcta cttttggggt cgttacggat tcgacaggca ttatgaggta
      781 tatttcgcga ctcatcggca gatgtaaaat gccagttaaa tataactgca aaaaatacaa
      841 attcttacgc attagctgcc taaaaaacag cctgcgtgat cttcacaaga ttgtttgcgt
      901 tttgctagaa ggtcttattt atcagcaaac tacgtttggc tactgtctag ttagttaaaa
      961 agagatttat agactcgcta tgtgagggct tgagttatgt gtcatcacct agttaaatca
     1021 atacataacc tatagttgta gacaaatata ttagcagatg tttggacgtg ggttcgactc
     1081 ccaccggctc catatcattt taccataact tactatacat acttaaaacc ttgttattac
     1141 aaggttttaa gtatgtttct ttgttcatac attgcctcta tcttaggaaa aaaatactgg
     1201 gaacagactc taacattgaa ttaggccacc tcattagctc actctttgtc aatatccgag
     1261 aataagttct ttatcattct aatttataga atttattaaa aaaggtaagg tttttagagg
     1321 aaaaaatgtt ataatctatt tagtctacga ttggaggcta gttatggcta gtatttaaaa
     1381 aaaacattta gagaaacgtt tagagcttaa aaatgatcct atgaggacag atatgagtac
     1441 tggtgccagt taccctaaat atggattctc ggtaggaaag tacacatatg gatatcaaca
     1501 attcttttac gaaggcgtta acttaaaaga aattggagca ttttgctcaa tagcccagaa
     1561 cgttactatt actggtctga atcatccaac cgatcacatt accactaacc cttttattta
     1621 ctataagtca aggggattta taaatgagga tagggcggac ttaatagatg aaaagaaaaa
     1681 tggaaaagtt attattggca acgatgtctg gataggaact aatgtcacta tacttccatc
     1741 tgttacaata ggtaatggag caattattgg agcaggttca gttattacca aagacattcc
     1801 tgactatgct gttgtcgctg gaacacctgc taaaattatc aaatatcgct tttcagagga
     1861 agaaataaca ttattaaatg catctcagtg gtggaattgg tcagatgaag ctattaagga
     1921 acatattagc gaattttcag ataagaagga attcttcaat actctaaaaa gtatttccga
     1981 aaacaaaaat cataaactct agaataactt catcaaatag cacactaaaa atactactca
     2041 gcataatatg agtagtattt ttagtgtgct attttgctag tttatttagc accatgttta
     2101 gactaatgca attctaggaa agctacttag tctctctgct attatcatga taagctcccc
     2161 aaaatatggt tactaataat tttaaagatt tgttaaataa ccttttttta tcatcatcta
     2221 gttctctaaa gctatgaaaa gctttcatca tttttgctct ggaagccaag ttgaagtcat
     2281 ccaaactaga tacgccactt gataggaagc tatcaaataa taaatcaaaa aataattttc
     2341 tttcttgact ttttaattca gccatccact gttgaaatgt cttttcaaaa caaatacttt
     2401 ttagcgatag tcctgttcca gtctttaaaa tagctggttc ttgaatttcc caagttgtaa
     2461 catcgtgttg cagaattcca cgcgcattgc tactaataat tagcggctct atatcactat
     2521 aaagcatcat accaacaata gattcttctg gtagtaagcg aatgcacttt tgtttagtag
     2581 ttttatactc tgtagtaggt aacaaagttt tttgtagtcc tggggagtca ataagccaaa
     2641 tcaaatcaat ttttgctttt aaatctggct ttgtaaacat tgcagcgtac aaggctaaat
     2701 tacccccctt agaatgacct gagagaacta ctttatcaaa ataaggcagt attgtattta
     2761 agtatttaat cgctgaacga tgagccgata tttctctcat ataagtcaat ttaaaatctt
     2821 ctttccaacc aataaggttg gcatcagtac cacgaaatac aacctgatga taattaatgt
     2881 taggaatagt aaaaaccata gctgcaaatt gtttttcaaa ttctaaacta atctcagctc
     2941 gataataaga taaagttaag tttttaaaac gtttcgaggt catcattaac tgacataaat
     3001 catgtcgttg tgatgtgaac ataaagctat atgatttagc catagtttga aggtactcat
     3061 gatataactc acagactaaa actgacttca tttctgtagt gtttataagt ttttcaaaag
     3121 aaatatagcc aaattcattt aaacaaagaa tgtcaacatc gtttaatgct aattcatcaa
     3181 aagttaagtt tgaattattt tttaaataag ttattatatt tgacatcacc gtttattctt
     3241 tctactgctg aataatttcc gaacttcatc taaacctatc atgataaatg ggcagcttat
     3301 taaatagagc caatgtgata aacctaggct tgctgtgttg aataagttat ggaatagtgg
     3361 cacgtatact aagactaaaa atgctaaaat ctccattatt ataccaaaat taattaattt
     3421 attaccaaaa atagatagtg ctttaataga ttgatagctc gttctgctat tcataaccat
     3481 acctatctgg ctaaagataa tagctccaag tgtcatagtt gtcgcttctc tgtacggtat
     3541 gccgtttgcg acgaaaaaag taaaattacc ataacgtaag taatgagccc agaaaaaacc
     3601 acccatagct aatacggatt caatagtgcc gtaccaaagg aaagatttta taagtagccc
     3661 tttgtccaat aagcgatctg ttagccgtct tggaggacga ttcataacgt ctgtttctgg
     3721 tggttccacg cccagaccta aagcgggaag catatcggtt cccaaatcaa cagcaagtat
     3781 ttgcattaca gtcaatggta aaggaataaa acctttagaa aaaaggaaga atgccgacgg
     3841 aacagcctca ggagtattac tattaaaaat atacgtcaag aatttcttaa tgttttgata
     3901 aacagcccgt ccttcttcca cagcatgtac aatagaagca aaatgatcat ctgtcagaat
     3961 catatcagct gattctttag caacatcagt accggtaaca cccatggcta caccaatatc
     4021 ggattttttt agcgcaggcg catcattgac cccgtctcct gtaactgcta ctacttcacc
     4081 catctcctgt aatatcgtta ctactcgata tttttgttct ggtgctacac gagcaaaaac
     4141 gacttcacct gacaattctt ttttcaattg gctatccgtc atttctgata actctaaacc
     4201 tgaaattact ttagcatcat cgttacgaat aataccaata tttttagcga tacttagtgc
     4261 cgttaatccg taatctccag tcaccataat aatacgaatg cttgctgcat gacatttatc
     4321 aatagcttcg cgaacgcctt cacgaggtgg atcactcatt gctatcaacc ctataaacac
     4381 catgtgttgt tccaaatttt cttgcgtcca ttgttctttt gaaaagcctt caatatcgca
     4441 gtatgaaaca gctaagacgc gaagtccatc tttagcaaat gtatcgttag ccaactgtat
     4501 tttattacgt tcttccttgt ttaaggggat aactttgcca tcagataaga cataatctga
     4561 taaatcaagt atttctttcg gcgcaccttt tgtaattgaa atttttttat ccttttcatc
     4621 gcctcctaag ctatgaatag ttgtcatgcg ttttctaacc gaatcaaaag gtaattcttt
     4681 caaacgtggt gcaaacttac gattttcttg tatgtttatc cctgattttt ccaataaaac
     4741 attcaaacaa gcttctgtcg gatctccaag tacagtataa gttgaacgat catcatttgg
     4801 tggtaatact tgagcgttgc tacagagatg agcaaaacga ataagttttt ctaagtctcc
     4861 tctatcacta ttaccaaagc aaatattgtc accttcaaaa agaatctgtc cttctggagc
     4921 ataacccagt cctgtcactt ggtaactctt tccattttgt cacaaatgat tgacggtcat
     4981 ttcattttgc gttaatgtac cagtcttatc agaacagata actgaggttg ctccaagagt
     5041 ctcaactgaa gaaagttttt tgacaagtgc gtgttcttta gccattcgtt ggactgccat
     5101 agctagagaa ggtgtaacgg taggtagtaa accttctggg ataaatgcaa cgatcatccc
     5161 caaggcaaag ataaatgatt ttgacactgg ctccttaaca aaaaaagtcg ctgctaagaa
     5221 aaagataatt cctaccgtaa ttgaaatgat agaaatctgt ttggtcaacc gatcaagttc
     5281 tctttgaaga gggctttttt ctgatttcat cccttgtgtt aaatcagcga tttgaccaaa
     5341 ttgagtttgc attccaattg ccgtaacaac cattgttgcg ctaccactgg agaccgtcgt
     5401 tcctgcgaaa accatattat cacattcaat caatgtttta gactggtcgt tttcaacatt
     5461 gctatcttta taaataggat ttgattcccc tgtcaaggct gattggttta cttgcaaatc
     5521 tgttgtttta atcaagcgac catctgctga aatacggtct ccttcttcaa taagaacaat
     5581 atcgcctggg actagttgct ctgataatat cttttcttct gagccttttc taagaacacg
     5641 tgaataagat ggtaacattt tctcgagagc ttgtgttgct tggcttgcac ggtattcctg
     5701 aatgaaacta aaaataccat taatcacatt aaccatccaa atggcaagac ctagctctaa
     5761 gctattagaa acaatagcca caaatcctcc aacccataat agtattgcca taagactagt
     5821 aaaatttttc agaaacaata aaataatcga ttcttgcttt acttcagata acaggtttaa
     5881 accatatttt ttctgacgta cagcaacctc ttcatctgat aaaccacctt gagttgtccc
     5941 taattcttcg aaaacctctt ctaactcctc tttagcaaaa cgtgtgtttg tatctgaaac
     6001 attatttgta gaagccatat tctagtctcc ttctctaaaa cgttttcatt tttgatacct
     6061 atattatact cttttttcag aaaataaact gatgaagaat acacctgcca ccaattcttt
     6121 ttgattaata aaaaacgact taagatatac ccttaagtcg ttttttatta atcaaaaagt
     6181 gataggtagt ccccatagcc tctctttgcc atctggtcat aaggaataaa atctaaggca
     6241 gctgaattga tacaatagcg taaaccacct gcatcaaccg gtccatcatt aaatacatgc
     6301 cctaaatgag agtcagcttg acgactccta acctcaatac gatgcattcc atgagagtga
     6361 tcttgatgat ttgtcaccat tcgattctca attggttttg aaaatgctgg ccaaccacaa
     6421 ccagattgaa atttatctaa agaagagaat aaaacctctc cactgacgat atctacatat
     6481 atcccctctt caaaaaaatc atcatacttc ccagtaaaag catgttccgt agctgaattt
     6541 tgggttactt gataagctgt atgtccaata cgctgacgta attcttcttg tgtttctttc
     6601 attatagtat ctcctccatt tgagtttgtt gaggtttaaa acctaatttt tcataaaaag
     6661 agaccgctct ctgattatca ttccaaacat ttaaagttat attataacac ccatgtttct
     6721 tagcatattg tcttgcaaaa tccatcaact tttgtccaat ttgctgacct cttacttcct
     6781 catcaatgca caaatcatct atatagagag ttttaaatga ctttcgaggt aagtcacgtt
     6841 tttcttgtaa ttgaagaaac aagtgggcaa caactttacc ctcgtctgat tcataaacaa
     6901 agataggctt tgattcgtct atcagcattt ctttcaattg ttctttagaa aacttacttc
     6961 ctcttgtatg aaaaatatct gggcgaacct catgatgtaa cattaaaatt tgtttcaata
     7021 gctctgcaat atcaggcaaa tctttttctt tcgctctacg aatcatatct actcctaacg
     7081 tttaccattc cattcagcat aaaattcggc taaatatagt tctaaaaaat catgacgttt
     7141 ttgagcaagc attttccctt gttttgtatt cataaggtct tttaacttta ataatttttc
     7201 gtaaaaatgc atgatagctg tatcctgacc attacgatac tcttctaaag tgaggttttc
     7261 tcgtggtttt aagttagggt catgaattaa acgtccttta ctccctgaat aagccatcgt
     7321 tcttgcaata ccaatagccc ccatagcatc taggcgatct gcatcttgaa caatttgacc
     7381 ttccaatgtt ttcatagtca atccagttcc tgctttgaat gaaatatttt caagaatatc
     7441 aaggatatgt tctacttctg ctatggcaag atcttgacta tataaccatt gagtcaattc
     7501 atacgacgct tgcttactat cttggcagat tttgtcatca ataatatcat gcaaaagtgc
     7561 agccatttga caaataaagg tattagcccc ttctttgtgt gctaattcta ccgcaagatt
     7621 tcttactcta acaatatgaa accagtcatg gccacttgga tcatttttta atttatggtg
     7681 aaccatattt tcagttgcat ttaaaatcac atcttctctt ttaaccatag tcatattata
     7741 gctaattttc taaaaaaata aatgatttag catataaatg caaaatcctc caagaaattc
     7801 ttgaaggatt ttatatatta gctctgtaaa agattaatgg tgatgatgac ctacatgtgc
     7861 atgatctgtt tcttcatggt catgatgttc atcatgtgtt ttagggtttt cttttttact
     7921 atcagcattc gcttgagcag atttttcagt gtttttttgg tgattagtat tatctgaaac
     7981 ttgtttgtcc tctttagaag ccatagctgc tttttcagca agactaccat ttccaaccat
     8041 tgaaccttct acaactgctg gaaaattctt agctactgct tcaggaatat ttttttcatt
     8101 caatttcaat tgtgatgttg gaacattgtt atggctaaaa tcaagatttg taactgtttt
     8161 gttaggtgtt ttaatatcta aagattcaag tttattttta gaaacactta aggtctcaag
     8221 tgaagtataa ttattaacac cttcaagtga tgttaactga ttattagtca cattcaagaa
     8281 ttttaacgaa ccttgtttat ttttcaatac tagttactta atttggttcc cttcagcttc
     8341 tactttaaca atttcatcgc tctcttcaat accatctaat gatgctaatt tcgcattatt
     8401 aattgttaaa ttagatacct taggattttg ctttaagaaa cttagattgc ttgtattcgt
     8461 ctcatttaaa agaagcgtct caagactagt tgtttttaga gtcgataagt ctgcggattt
     8521 attgtttgat aaatctagaa ctttcaaatc ttttttacca gaaagtgcac ttaaattctt
     8581 cacattatta tgatcaaggt gcaattcttg gagttttgtc aagtttgata acggtgtcaa
     8641 gtcagagata ttgttatgac tcaaaactaa gaattgaagg tttggtaatt cagccaatgg
     8701 ttttagcgaa gtaatgccat tattagctgc agcaataaga cttaattgtt tatattttgt
     8761 aaggaaagat aaatccttaa taccgttttg tgaaatatcc aatccttcta atagtggcat
     8821 cttatcaagg aaagaataat ctgtaatacc agtatttgtc atccaaagct gtttaagttt
     8881 tttgaattgc aaaactggcg tcatatcatt aatcggagta aacccaattc ctagaacttc
     8941 aatattaggc atcaatgata gaccaaaacg ctggaatgga ttggttcttt gtccaatgtt
     9001 aatcttagta acagttgcaa gccactgacg cattttttca ggattagttt catttgatgg
     9061 gaatggtgtt ggagcatctg catgcagaat atcctgaata atctcatcat caaaaccaat
     9121 ttgttttaga gttgccatac caactttatc ttttgcatga gagtgatcgt ggtgtccatc
     9181 tgggataggc ttcccaattt caatatcctt acttgctatg gtgtgtgagt ggtcatcatg
     9241 tggatactta aagatgatac ttccatcctc tgacgttaat acttcaattt gattaggatt
     9301 taaaccaagc ttctctgcta aataaattaa tttcttttga tcttcatcag ataatttatt
     9361 tgacttagtg tgctcgtgtt cttcatgctt atggtcggac tcatcatggt gatgaccttt
     9421 gtgatgatct tcatcatcat gatgtggatt tggaattggc ttacttagat caatatcttt
     9481 ggccataata acatgtgtat gatcatcatg tgggtattca aatccaattt caccatttag
     9541 tgttttaagg actctgatac gtgaaatatc taaatgtaat tcacgagcaa ggtaagctag
     9601 cttatctttg atgtccttag gtaaagtatt ttcagttctt ggctttttac ttgatggtga
     9661 ctgtttttta gaatcagctt tcttttgtgg ttggtaacgg tcagcaattg atccccaccc
     9721 cccttttctt aaatcagcaa aactaataaa atgcagatga ttatgatgtt ctactaaaat
     9781 acctgttggg gtagtgcctt ttattcctcg tccattaaat aagaaaccat tacttgttgg
     9841 gtagtgaagc cctgcaattt tagaactatt aaccctgtaa tggctattac tttgatttcc
     9901 taaacgtgaa acagctctac ttgcttgtac ttgcttttgc tgagataaac ttgcttttgg
     9961 aatatagtgg aaatgatcac catgtctgac cacgtagcca ttagcatctt ctgaaacaat
    10021 atctgcggga ttaaaaacat agccatcatt taatgtatta acagctaccg cccctgcttt
    10081 aaagtttctt accgcttgat ttttattagt ttttgtatta gcattaggga ttaaatagct
    10141 aaattttgaa ccttttaaat cagagtaaaa aataaaatga gaatgatttc catgtgctac
    10201 aacgattcca gaatctgtat gagataaaat cttcgaatct ttagtcagta aaaagccatc
    10261 atcagttgga aagtcaaccc ctgcaacacc cttgcgtttt ttatgacttt ccttaacaga
    10321 gactttcttt ttattagctt ttttcttagc aacctgcttt gttttaatat gatgagaatt
    10381 tttgtgttct tgagtatgac atgccgtaag agcaaaatgg catgacagga caagaccagc
    10441 tagagcaata attttttgac ctttcatgag tcctccataa atgttttttt cgttttagtt
    10501 aactagttaa ttatatcaga tgtttttctt tttgcaatac tatttgccat ctttttatta
    10561 tttagttact aacaaaaact catttatagc tcatttatat ttaaacaaat atattataaa
    10621 gccatattat ttgataattg cagacaaaga aaaagtaact taagcacgaa gctaagttac
    10681 tttaaaatag ataaatgata tcatctttct gaattatttt ttgtcatcat cgtccattga
    10741 taaaacactt aagaaagctt cctgaggaac ttcgacggaa ccaattgctt tcatgcgctt
    10801 tttaccagct ttttgttttt caagcaactt acgtttacgt gaaacgtcac caccataaca
    10861 ttttgctaag acatttttac gtagtgcttt tatatcagaa cgggccacaa ttttttgtcc
    10921 aatagctgct tggataggaa cttcaaactg ctgacgagga atgatttttt tcaacttatc
    10981 aacgatcaat ttaccacgtt cataggcaaa ttctttgtga acaataaagc ttaaagcatc
    11041 cactttatca ccatttaaaa gaatatccat tttaactaat tgtgagcgac gatattcgga
    11101 aatttcataa tcaaaactag catagccacg tgttgatgat ttaagcttat caaagaagtc
    11161 aaacacaatt tcagctaaag gaatttggta aataacatta acacgattat catcaatata
    11221 atccattgtt acaaagtcac cacgtttacg ttgtgctaac tccattacag caccgacaaa
    11281 ttcttgagga accataattt gcgccttaac atatggttct tcaatgctgt caacacgagt
    11341 aggatcagga aattcagatg gattcgaaac ttcaagcatt tcaccgtcag ttgtattaac
    11401 atgataaact actgatggtg cagtcataat aagatcaata ttgaactctc gttccaagcg
    11461 ctcttggata acgtccatat gtaaaagtcc taagaaacca caacggaaac caaaccctaa
    11521 tgcttgtgat gtttccggtt caaactgtag actagcatca ttcaattgta atttttcaag
    11581 ggcttcacgc agatcgttat acttatttga ttcaattgga taaagaccgg caaaaaccat
    11641 tgggttcatt tgcttatagc catgcaaggg ttctattgct gggttattag caagggtaat
    11701 ggtatcacca acacgtgtat cagcaaccgt tttaattgag gcagcaatat accctacatc
    11761 tccagttgct aaaaaatcac gtccaactgc tttgggagtg aaaataccaa cctcagtaac
    11821 gtcgaaggtt ttaccattag acatcatttg aatcttatct cctggcttaa ccataccatt
    11881 tacaatcctt acttgaagaa ttactccacg ataagcatca taaacagaat caaaaattag
    11941 agcttgaaga ggagcatcta cttccccagt cggtgcagga actttttcaa cgatctgctc
    12001 taaaatttct tcaataccaa tccctgcctt agctgaggca agcacagctt ctgaagcatc
    12061 tagaccaata acatcctcta cttctgcacg tacacgttct ggatctgctg ctggtaaatc
    12121 aattttatta ataactggta gtatctctaa atcattgtct agagccaaat aaacatttgc
    12181 cagagtttga gcttcaattc cttgggctgc atcaacaacc agtatagcac cctcacaagc
    12241 tgccaatgaa cgtgatactt catatgtgaa gtcaacatgc cctggcgtgt caatcaagtg
    12301 aaaaatgtaa gtttcaccat ctttagcagt ataattaagc tcaatagcat ttaactttat
    12361 agtaattcca cgctcacgtt ctagatccat agagtccaac agttgcgcct gcatttcacg
    12421 actagaaacc gtttctgttt tctctaaaat acggtctgct aaggttgatt ttccatggtc
    12481 aatatgagcg ataatcgaaa agtttctaat cttttcttga cgtttcttca aatcttcaat
    12541 attcattcta tttcctatac aatttgatat gtttaacttc taaagtatta aaacagtcct
    12601 atagactcta gaatattata tcaaaaagaa gaccgatgta aaagagaatt aacacttgaa
    12661 catcaatctt cattaagtct attagttatt gaaccaaatg gctatctcgc gagttgcaga
    12721 ttcaggtgag tctgacccat gaacaatatt acatgtcgct tggtttggag agggagcttg
    12781 tgcaaaatct cctcgaatag ttcctggaag ggcatctttg gggttagtac tccccatcat
    12841 ggtgcgccaa gttgaaatga cctcttctcc cgaaataact ccaatgatta ctggaccact
    12901 agtcatataa tctactaatg ttggaaagaa tggtctatcc gtcagttctg cataatgtct
    12961 tttcaaaata tctgcatctg catatctaac ctctaagcga tcaatagaga agcctcgacg
    13021 ctcaatacga ctaataactt ctccaataaa accacgtttt acaccatcag gttttatcat
    13081 aaaaaaggtt tgttccataa tattagcacc tcatttcttt ctccactatt ataacatgaa
    13141 aatatgtttt ctgtaacttc caactgtatt tcaaaaaagg agtattggac actccttaac
    13201 tatattaata ggtgagatcc atcatagctg ttagttgatt atggataggt tctccactgc
    13261 tttccttgta agcttgcaag tattaatgac aaagaaaaca ccagcaatca ctaaaagaac
    13321 caacgctgtc aaactaataa ttaagtaaag cgtagaacaa cctagccctt taaccataag
    13381 gttggaatta tagcctttca tatctctttt attgacacat tctctttgaa tccgaaaaac
    13441 aattcgaatt ttctactaat agacacacct ctaaatgacg ttcatatttt ttagtcgcca
    13501 atatataagt ttcccaatca aactgataaa ttggggtgat ttcgttagct tggccaacta
    13561 agttctgaat tacgtcttga attaatctaa ttaccgcctt cccccacatt ctctgaaaat
    13621 ttatttcagt ataacaaaaa actagaacac cacaatgatg tcctagcata ataacagcac
    13681 agctctaaaa ctatccatct cggtgagatg agaattagct tgttttggaa ccattcgaaa
    13741 cagcacagct ctaaaactaa attccgtcat cttcaattcg tcaagtagtt ttggaaccat
    13801 tcgaaacagc acagctctaa aacatataag cgatatggaa gcattggtaa aaagttttgg
    13861 aaccattcga aacagcacag ctctaaaaca tgagcctaaa ttcatagagt ggaacatatg
    13921 ttttggaacc attcgaaaca gcacagctct aaaactatca gtccacttat aatcaagata
    13981 gtttggtttt ggaaccattc gaaacagcac agctctaaaa cagttttaac attaaatcac
    14041 gtcttaaata agttttggaa ccattcgaaa cagcacagct ctaaaactac cgcaataaga
    14101 tcaccaatgt tgtttgagtt ttggaaccat tcgaaacagc acagctctaa aacgaacaag
    14161 atgtagatat gaaattaaca tttgttttgg aaccattcga aacagcacag ctctaaaact
    14221 caaccaccgc cgcataatcg aaatcatccg ttttggaacc attcgaaaca gcacagctct
    14281 aaaacaaata aaagccataa taataatggc tactagtttt ggaaccattc gaaacagcac
    14341 agctctaaaa cctccaagtg ctttaaagtc atttattttt tgttttggaa ccattcgaaa
    14401 cagcacagct ctaaaaccca tcatcaacct gaatgactcc acatttcgtt ttggaaccat
    14461 tcgaaacagc acagctctaa aacgaccctt atcaaaccca agcagaagta actgttttgg
    14521 aaccattcga aacagcacag ctctaaaacg atggtcgagc acttagaaaa ccaataaaag
    14581 ttttggaacc attcgaaaca gcacagctct aaaacgcttg tttcgctgtc gctcttgttt
    14641 gacgggtttt ggaaccattc gaaacagcac agctctaaaa caagcacaag aagcaactgt
    14701 tagaagacat agttttggaa ccattcgaaa cagcacagct ctaaaacaca gctgaagagt
    14761 tagaaaaggc taatgttgtt ttggaaccat tcgaaacagc acagctctaa aacctcgtag
    14821 aaaattttct tttgagcttt cgtaatcgcg ccattcgtct cagcaggact tcagtttcga
    14881 tgattccttg ttattactgt gcttttacta atattatacc atattttcgc ctatcaagaa
    14941 ataatcctta tcaataacat attgcggtaa atcatagagt cttctaggtt ctagaaagag
    15001 tactgacttt tgcattaaat tgatgtattc acataatttt ataacttcat ctttggtaag
    15061 ataagctccg ctattaacaa aaaccaagag attctttttc gttaaataat ggtaaacttg
    15121 tataatttca aaacattttt caaagatagt gtcgctctgt gtctcaattt tgactcccag
    15181 tgccttaatg agttctaaaa tcgtaatttc atcgtattct aaatcaagct cattctctag
    15241 acactcaaat gcgataagtt ctgtaatagt agctgctaat ttttctacca ttgatttcac
    15301 ttctggcttt tcgttgaatt gattttctaa gtcaccatgt atcaacttta gaataggtgc
    15361 tgagttgaca tcgtatccta agatatctgt aaccagcatt aactctgttg ccttcagact
    15421 tttctgctta tcatcaaata atttcaaatg ttcatctacg tcatattgat aaaaatgttt
    15481 caccaatgaa gaataaactg aaacatcttc tatcgttaaa atcgtagcat tacttaacac
    15541 taatggttca tctaaaattg gaaaattaat cttgatcata agaatctcct aggaaaacca
    15601 agcgactatc agagttggca acacttgtgt tgcgttctcc atggaggtaa accattctcg
    15661 caaattgttt ttccgtaact gttaagagtg tgatattacc ctttttagga ttattcactt
    15721 taagccgacc tatcatagca ttattagctg tattattgag taataattta ctataaacag
    15781 aaaactgatg catgataaag ccttcgctca agagaaactt tctaaactta cgatacgcct
    15841 tccgttcttc tgctgtttca gtaggcatat caaacattaa aatcattcgc atataccgat
    15901 aactcatatc ctaaactccg gaattccatt cccatcacta tttagcgact taataacttt
    15961 tttggtatag tcgctgacaa tatttgagag atacatttct ttaccattgt agctgtatgt
    16021 ctctgaaaac atagaaaaga gttctctttt cattttgaca aaatcactct gcctattttc
    16081 ataaataatc ctatcaacga ttgggcgaaa tggttccata atatcgcttg ctaggttgaa
    16141 ctgattaaat tgattagcat gcttcaagcc aaattgtgtc atgcaaccac acttaacaac
    16201 ttcacgcgca aacatactca aaagcaatga atatccgtag tctaaaccag catttattgt
    16261 attatcctgc tctcttgaaa aatcatttcc aaaaagtgta ttgaaataaa tcctagcagc
    16321 atgcccctca cgattagaag gatcaaaagg ttctaagtca tggtagagat tcataataga
    16381 ctgggatttt tcaaagaaag aacactcacc gagataaaaa gactgattca aaattttttg
    16441 tgcaataatt gatgtccaaa catctgcttt gacatcttca atccatgaca tctgcctaga
    16501 taattgtaaa ctcgaatcat gtctggcata gtacggcatt aacatagctg ttggcaagcg
    16561 tttatcgtca caaaatataa ctaaaatatt ttcatcaacc aaacgtttaa tcagcatggt
    16621 cgacaaaacg atatctgttg tttccatgat tagaatgtca atctctgata gatgaatcat
    16681 ttccgtctga taagaatctt taaaaatcag atgattattt ttataagaga gcttagaatg
    16741 tgtatttaca acaacggttc gccaacctgc catatcaatc ttctcctaac ttacccaaat
    16801 caatacgtgt ttcataaagt ccagtaatag attgatgaat tagggtagaa ttaagtactt
    16861 ctttagttga tgtatagcgt tttctatcaa ctattttatc aaaaaattta aaagctgctg
    16921 gagctcctag actggtaaaa gtaaatagat tgataatatt attagcaagt tcatctactg
    16981 atatattttc cttattatct tggtaaagct tattgatttt ctctaaatta gcatctgcta
    17041 gaataactcg ttttgaaaaa tcattaatta attgaaggat gtcatcaaaa taagagacat
    17101 gttgatttac aaattcttgt ttcttctcaa tctcctctgg tttacctttt gactcattat
    17161 aacgacttgc aaggtataag aacttcataa attgtgttgg taaggctagc tcattacctt
    17221 tttgtaattc accagcacta gcaagtaatc gacgacgccc attttctaat tcgaacagac
    17281 tatacttggg caaaataatt agtttatcag ccctaatatt taaatagcct tttgattcaa
    17341 ggaaagctga tggatttttc tcaaatctgg acctctccat gatggtaatt cctaaaagtt
    17401 ccgtaactgt ttttagtttt tgtgctttac cctttttgat atcagctaca actaaaacag
    17461 agtaagctac tatcggacta tcaaaacctc catatttctt aggatctaaa taaatatcct
    17521 tcgtttttct tggaatcaac ttatctgagt taccatgcgc caagattgat tccttagaga
    17581 aaccacctgt ctgaatctct gtcttcttca cgatattgtt ctgagggtat gacaagactt
    17641 ttctaactgt cgcaaagtgt ttctttttat cccaaacaat ttcacccgta tcattattaa
    17701 cttcaatatc atcttttaca acaacggttc catccgctaa agttacctta gttttaaaga
    17761 agttcataat atttgaatag aaaaatagct tttctgtagc ggatttacgc gttttgtaac
    17821 tattatattt tggatagtcg ccgtagacaa attctggctc taactgagga tatttggtta
    17881 gaatagcttt agcaactact gcattaagat aggcatcatg tgcatggtga taattgttaa
    17941 cttcacgaat tttatagaat ccaaattctt ttcggaaatt tgaaaccaaa tttgacttca
    18001 aggttacaat tttaactttg cggatccttc tacctttact atcaagctca ttattaaagc
    18061 gttcatccaa gatacgggca acatgcttgg taatttgtcg tgtctcaacc aactgacgtt
    18121 ggataaatct tgccttatca tcggaagtta ggcctccgcg ctctgcctta gtcaaattat
    18181 catacttacg ctgactcatt aacttagcat caagtaattt tttccagaaa actttacaat
    18241 cttttacaat ttcaaggcta ggaacatcat ctgactttcc acgattttta gcagatgata
    18301 ccaaaacacg attatcaata gaatcatctt ttatgaaagc ttgaggaata atgtggtcaa
    18361 tatcatattg acttaaattg tcaatatcta gagcttcccc tgtatacata tctcttccgt
    18421 tttgtaagta gtaaaggaaa agtctttcat tttgcaacgc ttgattatcc gtaggatatt
    18481 ctttcaaaat attgccattc aagtcactag ctagattctt aacgccatca tcaagaagtt
    18541 tatagcgttg tcgagagtta cgacgacctt gatttgttgt ttgattctca cgcgccatct
    18601 caaccacaat ttgttcaggt tcgtatccca tgactttaac aagctcatca acaattttca
    18661 aactttgtag aattcccttt ttaatagcag ggctacctgc aagctcacct acaacttctt
    18721 ttagattatc tgaatgacta ccagcctgtg ccttactgat aattgatttg aaagatagac
    18781 catcatcatt tatcaactgc ataaagttgc gattagatct accatcatca ataagatagt
    18841 ccaagattgt tttttgactc tctttatctc gaataccatt gattaactta gcagacaatc
    18901 gtccccagcc agtatagtga cgacgataga gtttttttag ttgtgactct gtaaaaagat
    18961 ctttatagtt ttcaagacgc ttcttaatca tttctctgtc ttcaaataat gttagagttt
    19021 ggacgatatc ttccagaata gactcattat ctggattatc tagaaaatct ttgtctagta
    19081 ttttttcgag atcgtggtaa gttcccaatg aggcgttgaa agctttattt tctttatcta
    19141 gaccaataac atctactatc ctaaactcct catattcttt agccagaaaa tctagcaact
    19201 tcttcttgga taccttacga tgttccttga atactccatc aaagatttct tgtttaatat
    19261 tgctatcaaa aaaataagtc tcaccttgct catttttata tctaacctta gtcaactcat
    19321 tatagaccgt aaatttttca taaataagac tatgctttgg taaaactttt tcttcaggaa
    19381 gataaaaatc attgttggtc atacgatgga taaaagcttc cgcagatttt tctttatcaa
    19441 ccaagtcttc aaaattccaa ggtcgaatac tgtcatctgt tttgcgagtc atccatgcaa
    19501 aatcactctt ctcacgtgct agtggcccga tataataagg aattctaaag gtaaggattt
    19561 tttcaatcct atcttgattc tctttcaaga agggatagta ttctgattga cggcggataa
    19621 tagctttcag ctctgtcaaa tggacttggt gtggaattga gccattatca aaggtccttt
    19681 gttttctcaa gaaatcttca ttcttgattt tttcaagaaa attctcgcta tcttcttgct
    19741 tggtcaacaa ttttgacagg tatttataaa aagcttcttg attagtttta ccttcaatat
    19801 aaccagcgta gccatctttt gatgaatcag caaatatttc ttgatatttt tccggcaatg
    19861 aagcttttac gaattgtttt aactgtttca agtcctctct atgttcatca taacgctgaa
    19921 tcatagaagc tgaaagtggc gccttggtac tgaggtcgat tactgtaaga atgccagaca
    19981 aaaggacact atcatataac tttttcgctg ctgagaataa gtctgcaaat tcatcaccaa
    20041 tctgtccaag aagattttct aaatcttcat cgtagctatc cttagcgaat tgaagcggcg
    20101 ttttatcctc caaattgaaa tatttcttga agtcagcttg atttccgaca atcaatttca
    20161 aaaattctgc aaaaatgcca gtagattttt ggttaggata ctgcgctaaa atacgatctt
    20221 tcttcgcaga cttgctaatc ttatctgtta gtattgcctc tacgtcaacg ttttgagata
    20281 acaaatcatt attttcaaaa gttgtattaa agatttctaa aaaatcttga tattgttttg
    20341 aaatgtctgt attcctgaca tcaaagctat catcctcaat taggaaatgc cctctaaatt
    20401 taatgatatg agctagagca atataaataa gacgaaggtc tgctttttct ttcttgtcag
    20461 ctaattcttt tctcaaatga tagattgtcg aaaatttttc atgataatct ttctcttcct
    20521 gcaatgttgc aaagatagga tacttgctcc ctctcttatc ttcctcaact agaaaagaat
    20581 cctctaatcg atgaaagaaa ctatcatcaa ctttactcat ttcctctgca aaaatttctt
    20641 gtaaatatag aatacgattt ctacgacgtg tataacgacg acgagcagtt cgcttcaagc
    20701 gtctatctgc agcagtattc ccgccatcaa aaagcagagc acctatgaga ttcttcttaa
    20761 tatattcttt atcagtgttc cctaaaactc tcatcttctt agcaggtact ttataatcat
    20821 ctgtaataat gctccatccg acggaattag taccgatgtc aaggcctatt gaatatggct
    20881 tattcataac ttttctcctt ttatttaaaa caccattcta aaaacaacac ctttaaaaca
    20941 tactttaatt atatattatt tttttgaatt ttaaaagcat tatctttatc tttcatctgt
    21001 tatttgttaa aatatcctaa agtatgtttt aagttttaaa gctttgctga tttttatttt
    21061 ttacttgatt attacactaa aataacatat aataattttg ttggaattat tcgaaacagc
    21121 acagcacgtt aaaataaggc agtgattttt aatccagtcc gtattcagct tgaaaaagtg
    21181 agcaccgat
//
LOCUS       DATRNK010000032        20730 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.032, whole genome shotgun sequence.
ACCESSION   DATRNK010000032 DATRNK010000000
VERSION     DATRNK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20730
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.032"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..476
                     /locus_tag="VBC52_001180"
     CDS             <1..476
                     /locus_tag="VBC52_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882770.1"
                     /translation="CFYHLDRPLLRYNFYHERENILATLKIRFLYSIKLNFPIFVALN
                     ICFGTYYFNFFTPKIDQIIILVITQAISMILFSFYFLYLYFLLQPFTEGLKSKSAMYN
                     ILTFIIYYMGYQLFTFTKSITMPILLVSLGIIVVILIIGYLAVVVFAPKTFRLKS"
     gene            672..1841
                     /locus_tag="VBC52_001181"
     CDS             672..1841
                     /locus_tag="VBC52_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO6882771.1"
                     /translation="MAWLGNFFTGASFSLVMPFMVLYVEQLGAPSNKVEWYAGLSVSL
                     SALSSALVAPLWGRLADKYGRKPMMVRAGLMMTFTMGGLAFIHSVTGLLILRILNGIF
                     AGYVPNSTALIASQAPQEESGYALGTLATGVTGGMLIGPLLGGLLAEWFGIREVFLLV
                     GTILLISTLMTIFMVKEDFKPISNEETMPTTEVFKSVKSLQILIGLFVTSMIIQISAQ
                     SIAPILTLYIRHLGQTENLMFVSGLIVSGMGFSSILSSPKLGRIGDRIGNHRLLLLAL
                     LYSFLMYVLCSLAQTSLQLGVIRFLYGFGTGALMPSINSILTKIAPRQGLSRIFSYNQ
                     MFSNLGQVLGPFVGSAVSIHLGFRWVFFVTSFIVLANFVWCFINFRKYIRVKEIV"
     gene            1838..1984
                     /gene="rpmG"
                     /locus_tag="VBC52_001182"
     CDS             1838..1984
                     /gene="rpmG"
                     /locus_tag="VBC52_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6882772.1"
                     /translation="MRVKINLKCSSCGSKNYLTSKNKSNHPDKIEVPKYCPKERKVTL
                     HIES"
     gene            2031..2267
                     /gene="secG"
                     /locus_tag="VBC52_001183"
     CDS             2031..2267
                     /gene="secG"
                     /locus_tag="VBC52_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEO6882773.1"
                     /translation="MYNLLLTILLVLSVLLIISIFMQPQKNPSSNVFDSSGSEALFER
                     SKARGFEAFMQRFTGVLVFFWLLIGLVLSILSSH"
     gene            2380..4785
                     /gene="rnr"
                     /locus_tag="VBC52_001184"
     CDS             2380..4785
                     /gene="rnr"
                     /locus_tag="VBC52_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEO6882774.1"
                     /translation="MKEKIIKYLEEHGKTSVNDLAAALDMAGAKAFPKLIKTISNLES
                     HRQLRFDDNGSLSLRKKEAKKKEITVRGLFRANKAGFGFLSIDQDEDDMFIGKNDIAY
                     AIDGDTVEAVVKKPADRLNGTAAEARVVNIVERSLKTLVGKFVLDDERPKYAGYIKSK
                     NQKINQKIYIRKEPVVLDGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVLE
                     VLESMDIVSEFPEDVIAEANAIPDAPTEKDLIGRVDLRQEVTFTIDGADAKDLDDAVH
                     IKLLDNGHFELGVHIADVSYYVTEGSALNREALSRGTSVYVTDRVVPMLPERLSNGIC
                     SLNPNLDRLTQSCIMEIDQNGRVVNHQITQSVINTTYRMTYTAVNDIIAGDEEICSEY
                     ESIVPSVQHMVTLHHILETMRTRRGALNFDTSEAKIMVNDKGMPVDIVIRNRGIAERM
                     IESFMLAANETVAEHYARLKLPFIYRIHEEPKAEKLQKFIDYASIFGVHIQGTATKIT
                     QPALQDFMKKVQGQPGSEVLSMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRRY
                     PDLLVHRMIRDYGDKAMDKADHFANLIPEIATQTSSLERRAIDAERIVEAMKKAEYME
                     EYIGEEFEGVVASVVKFGMFVELPNTIEGLVHVTTLPEYYHFNERTLTLQGEKSGKVF
                     RVGQQIKVKLIRSDKETGDIDFDYLPSDFDIVEKVSKSSREGRPNRSSKREHQHRISD
                     RDNKNKNTSKKKASRKPQRNSDSKSHHHKDDRTTGSTKKKTKKPFYKGVAKKGQKRKS
                     KKG"
     gene            4788..5255
                     /gene="smpB"
                     /locus_tag="VBC52_001185"
     CDS             4788..5255
                     /gene="smpB"
                     /locus_tag="VBC52_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEO6882775.1"
                     /translation="MVKGQGNVVAQNKKAHHDYTIVETIEAGIVLTGTEIKSVRAARI
                     TLKDGYAQIKNGEAWLINVHITPYDQGNIWNQDPDRTRKLLLKKREIEKISNELKGTG
                     MTLVPLKVYLKDGFAKVLLGLAKGKHDYDKRESIKRREQNRDIARQLKNYNSR"
     gene            complement(5339..6727)
                     /locus_tag="VBC52_001186"
     CDS             complement(5339..6727)
                     /locus_tag="VBC52_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO6882776.1"
                     /translation="MSKNNNDHTQKSENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SISLTGPSIVLVYAITGAFMFFMMRAIGEMLYQDPNQHTFINFITKYLGPGWGFFSTL
                     SYWISLIFIGMAEITAVGEYVQFWFPEWPSWIIQIVFLAILSSINLIAVKAFGETEFW
                     FAMIKVIAILGLIATGIFMVLTNFDTGHGYHASISNITNHFEWFPKGKLNFFMAFQMV
                     FFAYLAIEFVGVTTSETANPRKVLPKAIQEIPMRIILFYAGSLLAIMAIFPWQQLPVN
                     ESPFVTVFKLAGIKWAAALINFVVLTSAASALNSTLYSTGRHLFQLANESPNALTKAL
                     KLDQLSRQSVPSRAIIASAVIVGASALISVLPGISDAFSLITASSSGVYISIYVLIMI
                     AHWKYRKSPDFMEDGYKMPAYKILSPITLLFFLFVFVSLFLQDSTYIGAIGATIWIIG
                     FGLYSHFKHKSH"
     gene            complement(6856..7782)
                     /locus_tag="VBC52_001187"
     CDS             complement(6856..7782)
                     /locus_tag="VBC52_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6882777.1"
                     /translation="MALLSVIVPCYNEQETVSTFLTEIKKVESEMARYTHFEYIFVND
                     GSTDRTLELLKKAAKQFDNVHYLSFSRHFGKDAALLAGLEHTTGDFITVMDVDLQDPP
                     TLLPEMYLKLQEGYDIVATRRKDRKGEPLIRSLFAKLFYKLINQVSDTKMVDGARDFR
                     LMTKQVVDSILELNEVNRFSKGIFSWIGYDVAYISYENRERIAGKTSWSFFNLLKYSL
                     DGFINFSEIPLAIATWIGTLSSVLSLLAIIFIIIRKLLFGDPVSGWASTVTIVLFMGG
                     IQLLSLGIIGKYISKIFLETKKRPVYIVKEEG"
     gene            complement(7911..8303)
                     /gene="gloA"
                     /locus_tag="VBC52_001188"
     CDS             complement(7911..8303)
                     /gene="gloA"
                     /locus_tag="VBC52_001188"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEO6882778.1"
                     /translation="MPFLHTCIRVKDLDASIAFYQEALGFKEVRRNDFPENQFTLVYM
                     ALEDDPSYELELTYNYDHEAYDLGNGYGHIAVGVDDLETTYDAHQKAGYSVTKISGLP
                     GKPNMFYFIQDPDGYKIEVIRLSQFKAD"
     gene            complement(8391..9065)
                     /locus_tag="VBC52_001189"
     CDS             complement(8391..9065)
                     /locus_tag="VBC52_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6882779.1"
                     /translation="MTKEDIKKQVRRAFDFRMAIRVYNNNDIPKEDMEYILDTAWLSP
                     SSVGLEGWRFLVLDRQTIAKFRDKLKEVAWGAQYQLDTASHFVLLLAEKGAYYNADSM
                     INSLIRRGLGDPAALESRIPLYKSFQENDMKIDSERSLWDWTAKQTYIALGNMMTAAA
                     MIGVDSCPIEGFDYEKVNNILSKEGLIDDKKEAISCMVSFGYRLREPKHSRARKERQE
                     VVTWVE"
     gene            9332..10174
                     /locus_tag="VBC52_001190"
     CDS             9332..10174
                     /locus_tag="VBC52_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6882780.1"
                     /translation="METYTLSNTLNIPKIGFGTWQLTEGEEAYKAVTHALKVGYTHID
                     TAQIYGNEHSVGRAIRDSGLARESIFLTTKIWNDKHDYHLAKASIDESLQKLGVDYID
                     LLLIHWPNPKALRENDAWKAGNAGTWKAMEEAYKEGKVKAIGVSNFMKHHLEALFETA
                     EIKPMVNQIILAPGCAQEDLVRFCKGNDILLEAYSPFGTGAIFENESIKAIAEKYGKS
                     VAQVALRWSLDNGFLPLPKSATPKNIEANLDIFDFQLNEDDITTLIQLDSGIKPKDPD
                     NVSF"
     gene            10365..11087
                     /locus_tag="VBC52_001191"
     CDS             10365..11087
                     /locus_tag="VBC52_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6882781.1"
                     /translation="MRLDKFLVECGLGSRTQVKLILKKKQISVNGNSETSPKVQVDEY
                     RDEIKYNGTLVSYEKFVYYMLHKPKGVISATDDPSHKTVLDLLDKTARDKAVFPVGRL
                     DIDTTGLLLITNNGELAHKMLSPKKHVDKCYEAKISGIMTEDDILAFDKGIILKDFTC
                     LPALLEIVEVNQVKKQSLVKITIKEGKFHQVKRMVAACGKEVLELKRLSMGNLQLDKQ
                     LESGQWRRLTIKEIEKLEKYMQ"
     gene            11172..13211
                     /locus_tag="VBC52_001192"
     CDS             11172..13211
                     /locus_tag="VBC52_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEO6882782.1"
                     /translation="MKRKYFILNTVTVLTLAAAMNTSSIYANSTETSASVAPTTNTIV
                     QTNDSNPTAKFSSESGQSVIGQVKPANSVALTTVDTPHISAPDALKTTQSSPVVESPS
                     TKLTEETYKQKDGQDLANMVRSGQVTSEELVNMAYDIIAKENPSLNAVITTRRQEAIE
                     AARKLKDTNQPFLGVPLLVKGLGHSIKGGETNNGLIYAGGKISTFDSSYVKKYKDLGF
                     IILGQTNFPEYGWRNITDSKLYGPTHNPWNLAHNAGGSSGGSAAVIASGMTPIASGSD
                     AGGSIRIPSSWTGLVGLKPTRGLVSNEKPDSYSTAVHFPLTKSSRDAETLLTYLKKSD
                     QTLVSVNDLKSLPIAYTLKSPMGTEVSQDAKNAIMDNVIFLRKQGFKVTEIDLPIDGR
                     ALMRDYSTLAIGMGGAFSTIEKDLKKHGFTKEDVDPITWAVHVIYQNSDKAELKKSIM
                     EAQKHMDDYRKAMEKLHKQFPIFLSPTTASLAPLNTDPYVTEEDKRAIYNMENLSQEE
                     RIALFNRQWEPMLRRTPFTQIANMTGLPAISIPTYLSESGLPIGTMLMAGANYDMVLI
                     KFATFFEKYHGFNVKWQRIIDKEVKPSTGLIQPTNSLFKAHSSLVNLEENSQVTQVSI
                     SKKWMKSSVKNKPSVMAYQKALPKTGDTESSLSPVLVVPLLLACFSFVTKKNQKS"
     gene            13321..13899
                     /locus_tag="VBC52_001193"
     CDS             13321..13899
                     /locus_tag="VBC52_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6882783.1"
                     /translation="MKVKNKILTMVALTVLTCATYSSIGYADTSDKNTDTSVVTTTLS
                     EEKRLDELDQSSTGSSSENESSSSSEPETNPSTNPPTTEPSQPSPSEENKPDGSTKTE
                     IGNNKDISSGTKVLISEDSIKNFSKASSDQEEVDRDESSSSKANDEKKGHSKPKKELP
                     KTGDSHSDTVIASTGGIILLSLSLYNKKMKLY"
     gene            complement(13934..15175)
                     /locus_tag="VBC52_001194"
     CDS             complement(13934..15175)
                     /locus_tag="VBC52_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEO6882784.1"
                     /translation="MVLQDFDNLLKKYAQLIISQGLNVQKGHTLALTIDVEQVHLARL
                     LTEAAYEKGASEVIVDYTDDFITRQRLLHASDEVLTNVPQYTVDKSLALLNKKASRLV
                     VKSSNPNAFATVDPKRLSETTRATAIALEEQSRAIQANKVSWNVAAAAGREWAALVFP
                     ELKTSDQQVDALWDTIFKLNRIYEDDPIAAWDAHEAKLLEKATRLNQEQFDALHYTAP
                     GTDLTLGMPKNHIWEAAGSLNAQGETFIANMPTEEIFSAPDYRRADGYVTSTKPLSYA
                     GVIIENMMFTFKDGKIINVTAEKGQETIQRLIEENDGARSLGEVALVPHKTPISLSGL
                     IFFNTLFDENASNHLAIGSAYAFNVEGGTEMTSQELDEAGLNRSSTHVDFMIGSEQMD
                     IDGIRADGTAVPIFRNGEWAI"
     gene            15353..15481
                     /locus_tag="VBC52_001195"
     CDS             15353..15481
                     /locus_tag="VBC52_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEO6882785.1"
                     /translation="MAFGDNGQRKKTGFEKLTLFVVILMVLVTVGGLVFGAISAIM"
     gene            15542..16855
                     /gene="obgE"
                     /locus_tag="VBC52_001196"
     CDS             15542..16855
                     /gene="obgE"
                     /locus_tag="VBC52_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEO6882786.1"
                     /translation="MSMFLDTAKISVKAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFKVNEGLRTLMDFRYNRNFKAKAGEKGMTKGMHGRGAEDLIVSLPPGTTVRDATT
                     GKVITDLVEHDQEFVVARGGRGGRGNIRFATPRNPAPEIAENGEPGEERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVVSAAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYDDYVSINNELETYNLRLME
                     RPQIIVANKMDMPDSEENLAAFKEKLAANYDEFDDMPMIFPISSLAHQGLENLMDATA
                     ELLANTEEFLLYDETDMQEDEAYYGFNEDERPFEITRDDDATWVLYGDKLEKLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDIVRIGNFEFEFVD"
     gene            16881..17048
                     /locus_tag="VBC52_001197"
     CDS             16881..17048
                     /locus_tag="VBC52_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882787.1"
                     /translation="MGDKPISFRDKDGNFVSAADVWNAEKLEELFNTLNPNRKLRLER
                     EKLAKEKRIVK"
     gene            complement(17133..17483)
                     /locus_tag="VBC52_001198"
     CDS             complement(17133..17483)
                     /locus_tag="VBC52_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882788.1"
                     /translation="MKDYINRILHFIKEHMTYHVNFIDDFLDIKWEKVSNIHLRFWTT
                     IIAYLVIFILSISTVILNLVLLFQGFLTQNPIIYLLFFITLVCAFYFAYKFITYTPTI
                     VKNALQYIKKLKNV"
     gene            complement(17632..18372)
                     /locus_tag="VBC52_001199"
     CDS             complement(17632..18372)
                     /locus_tag="VBC52_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6882789.1"
                     /translation="MAELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLESITSGKVVVDGFELSNPKTDIDKARENIGMVFQHFNLFPHMSVLENI
                     TFAPIELGKESKEAAEKHGMELLEKVGLADKANAKPDSLSGGQKQRVAIARSLAMNPD
                     ILLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGRF
                     LEDGTPEQIFDTPQHPRLQDFLNKVLNV"
     gene            complement(18372..20555)
                     /locus_tag="VBC52_001200"
     CDS             complement(18372..20555)
                     /locus_tag="VBC52_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO6882790.1"
                     /translation="MKHKLKAFTLALLSIFFVFGGKVSAETVNIVSDTAYAPFEFKDS
                     DQTYKGIDVDIVNEVAKRAGWNVNMTYPGFDAAVNAVQSGQADALMAGTTVTEARKKV
                     FNFSDTYYDTSVILYTKNNNKVTNYKQLKGKVVGVKNGTAAQSFLEENKSKYGYKVKT
                     FDTSDLMNNSLDSGSIYAAMDDQPVVQFAINQGKAYAINMEGEAVGSFAFAVKKGSGH
                     DNLIKEFNTAFAQMKSDGTYNDIMDKWLGKDATKTSGKATGNANEKATPVKPSYKIVS
                     DSSFAPFEYQNGKGKYTGFDMELIKKIAKQQGFKLDISNPGFDAALNAVQSGQADGVI
                     AGATITEARQKIFDFSDPYYTSSVILAVKKGSNVKSYQDLKGKTVGAKNGTASYTWLS
                     DHADKYNYHVKAFDEASTMYDSMNSGSIDALMDDEAVLAYAINQGRKFETPIKGEKSG
                     DIGFAVKKGANPELIKMFNNGLASLKKSGEYDKLVKKYLSTASTSSNDKAAKPVDEST
                     ILGLISNNYKQLLSGIGTTLSLTLISFAIAMVIGIIFGMMSVSPSNTLRTISMIFVDV
                     VRGIPLMIVAAFIFWGIPNLIESITGHQSPINDFVAATIALSLNGGAYIAEIVRGGIE
                     AVPSGQMEASRSLGISYGKTMQKVILPQAVRLMLPNFINQFVISLKDTTIVSAIGLVE
                     LFQTGKIIIARNYQSFRMYAILAIMYLVIITLLTRLAKRLEKRLK"
BASE COUNT         6908 a         3390 c         3947 g         6485 t
ORIGIN      
        1 tttgtttcta tcatttggat cgtccattat taagatataa tttctatcat gaaagagaaa
       61 atatcttagc aaccttaaaa atacgtttcc tttatagtat aaaacttaat ttccccattt
      121 ttgtagctct aaacatatgt tttggaacct attattttaa ttttttcacc cctaagattg
      181 atcagattat catattagta attacacaag ctatatcaat gattttattt agtttttact
      241 ttttgtatct ttatttctta ttacagcctt ttacagaagg attaaaatca aagagtgcta
      301 tgtataatat tttaaccttt attatatact atatgggata tcaactattt acctttacta
      361 aatctataac gatgccaata ttattagtaa gcctaggaat tatagttgtt attttaatta
      421 ttggctattt agcagttgta gtatttgctc caaaaacttt ccgtttaaaa agttaatttt
      481 aattgtggta taataacaga ggcatgtgat aacataggtt tagttacata aaggaaatta
      541 taatggaaat aacatctcaa atagatgtta aatctcaata aaactgctaa gatagttagt
      601 ggtagaaaag tttagaaaga agatgatgtt ttatctcagt tgaaacggtt aactggaagc
      661 aaaatttaaa agtggcttgg ctcggtaatt tttttactgg ggcaagcttt tcacttgtta
      721 tgccttttat ggtattatat gtagaacaat taggagcacc tagcaacaaa gtagagtggt
      781 atgcaggctt atcagtatcc ttatcagcct tgtcttcagc tttagttgcc ccattatggg
      841 ggcgcttagc tgataaatat ggtagaaaac cgatgatggt aagagcaggg ttgatgatga
      901 cttttactat gggaggcctt gctttcattc atagtgttac aggacttctg attttacgta
      961 tattaaacgg catttttgca ggctatgtac ctaactctac agctttgata gcaagtcaag
     1021 ctcctcaaga ggaatcagga tatgccttag gaaccttagc gacaggagtg actggaggta
     1081 tgttaattgg tccactttta gggggacttt tagcagagtg gtttggtatt agggaagttt
     1141 ttttattagt gggaaccata ttgttaatat ctactttaat gaccattttt atggtaaagg
     1201 aagatttcaa acccatttct aacgaagaaa ccatgccaac tacagaagtg ttcaaaagcg
     1261 ttaaaagctt acaaatttta atagggctgt ttgtaacaag catgattatt caaatttctg
     1321 cacaatccat tgcacctatt ttaacacttt acattcgtca tttaggacag actgaaaact
     1381 tgatgtttgt ttccggatta attgtttctg gtatgggatt ctctagtatt ctgagtagtc
     1441 caaagctagg aagaatcggg gatcgcatag gcaatcatcg tttgctatta ttagctctac
     1501 tgtatagttt cctcatgtat gtcttatgtt cattggcaca gacttcatta cagttagggg
     1561 ttattagatt cctgtatggt tttggaacag gtgccttgat gccgagtatt aattcaatct
     1621 tgactaaaat tgctccaaga caaggtttat caaggatttt cagttataac caaatgttta
     1681 gcaatttagg acaagtatta ggtccttttg tcggttcagc agtttctatt catttaggat
     1741 tccgatgggt tttctttgtg actagcttca tcgttttagc taattttgtt tggtgtttta
     1801 ttaattttag aaaatatatt agagttaagg aaattgtgtg agagttaaaa ttaatttaaa
     1861 gtgtagctct tgtggtagta agaattattt aaccagtaag aataaaagta atcatcccga
     1921 taaaatcgag gttccaaagt attgcccaaa ggagagaaaa gtaacactac atattgaatc
     1981 ttaataagtt ttatgttaga atgtactttg accatagtgg aggaattaac atgtataacc
     2041 tattactcac cattctactt gttttatcag tattattgat tatctcaatt ttcatgcaac
     2101 cacaaaagaa tccaagtagt aatgtttttg atagtagcgg ttctgaagcc ctttttgaac
     2161 gtagtaaagc tcgaggattt gaagctttta tgcaacgttt tacaggtgtt ttagtctttt
     2221 tctggctact aatcggctta gtattatcta ttttatcaag tcattaatga tagctaaata
     2281 cagataagtg aggatggggg atagcccaac ctcacttttt gttagtagaa tggttgtaga
     2341 gtaataataa agtgactaaa gttactagaa aaggaaataa tgaaagaaaa aattataaaa
     2401 tatttggaag aacacggtaa aactagcgtt aatgacttag ctgctgccct agatatggca
     2461 ggcgcaaaag catttccaaa attaatcaaa acaatctcaa atttggagag ccacaggcaa
     2521 ttaagatttg acgataatgg aagtctttct cttcgaaaga aagaagctaa gaaaaaggaa
     2581 atcacagtaa gaggtctttt tagagcaaat aaagcaggat ttggttttct tagtattgac
     2641 caagacgaag atgatatgtt tatcggaaaa aacgatattg cttatgctat tgatggtgac
     2701 acggtagaag ctgttgtaaa gaaaccagcg gaccgtctta acgggactgc agcagaagcg
     2761 cgtgttgtta atattgttga acgtagttta aagacgcttg ttggtaagtt tgtcttagac
     2821 gatgagcgcc ctaaatacgc aggatatatc aaatcgaaaa atcaaaaaat caatcaaaag
     2881 atttatatac gcaaagaacc ggtcgtttta gacggaacag aaattatcaa agtagatatt
     2941 gataaatatc cgacacgagg acatgattac ttcgtagcaa gtgttcgtga tatcgttgga
     3001 catcaaggag atgttggtat tgatgtttta gaagtcttgg aaagtatgga tatcgtctca
     3061 gaatttcctg aggacgttat tgcagaagcc aatgctatcc cagatgctcc aacggagaaa
     3121 gatttaattg ggcgtgttga tttacgtcaa gaggtcactt tcactattga tggagcggac
     3181 gccaaagatt tagacgatgc tgtgcatatt aaattactgg ataatggtca ttttgaactt
     3241 ggagttcata tcgcagatgt atcatactac gttactgaag gctctgcctt aaatcgtgaa
     3301 gcacttagtc gtggtacgtc cgtatatgtc acagatcgcg ttgtaccaat gttaccagag
     3361 cgtctatcta acggtatatg ttcattaaat ccaaatcttg atcgtttaac gcagtcttgt
     3421 attatggaaa ttgaccaaaa tgggagagtt gttaatcatc aaattacaca gtcagtgatt
     3481 aataccactt atcgtatgac ttataccgct gtaaatgata ttattgctgg cgatgaggaa
     3541 atctgctcag aatatgagtc tattgtgccc tcagtacagc atatggttac tttacaccat
     3601 attttggaaa ctatgcgtac gcgtcgtgga gcgctgaatt ttgatacatc tgaagcaaaa
     3661 attatggtta atgataaggg aatgccagtt gatattgtta ttcgcaacag aggcattgct
     3721 gaacgcatga ttgagtcatt tatgttggcg gcaaatgaaa cggttgctga acattatgct
     3781 cgtctaaaat tgccatttat ttatcgtata cacgaagagc ctaaagctga aaaattacaa
     3841 aaattcatcg attatgcttc tattttcggt gttcacattc aaggaacagc aactaaaatc
     3901 acccaacctg ctttacagga ctttatgaaa aaagtacaag ggcagccagg aagtgaagtg
     3961 ttatctatga tgttattgcg ctcgatgcaa caagctagat attctgaaca caaccatgga
     4021 cattatggac ttgctgcgga atattacact cactttacta gccccattcg acgttatcca
     4081 gacttgttag ttcatcgtat gattcgtgat tatggtgata aagcgatgga taaagctgat
     4141 cattttgcta accttatccc agaaattgca actcaaacgt ctagtcttga gagacgtgcc
     4201 attgatgctg aacgtattgt agaggcaatg aagaaagccg aatacatgga agaatatatt
     4261 ggtgaggaat ttgaaggtgt ggttgcaagc gttgttaaat ttggtatgtt tgtagaatta
     4321 ccaaatacta ttgaaggatt ggttcacgta acgaccttgc ctgaatatta tcactttaat
     4381 gaacgtacgc tgacgcttca aggtgagaaa tctgggaaag ttttccgtgt tggtcaacaa
     4441 attaaggtta agttgattcg ttctgataag gaaactggtg atatcgattt tgattattta
     4501 ccatcagatt ttgatattgt tgaaaaggta agtaaaagtt cgcgtgaggg tcgtcctaat
     4561 cgaagttcta aacgagaaca tcagcataga atttctgatc gtgataataa gaacaaaaat
     4621 acatctaaga aaaaagcatc tcgaaaaccc caaaggaact cggatagcaa atcacatcat
     4681 cacaaagacg atagaacaac aggttcaacc aagaaaaaaa cgaaaaaacc tttctataaa
     4741 ggcgttgcta agaaaggtca gaaacggaaa tctaagaaag gttaagtatg gttaaaggtc
     4801 aaggaaacgt tgtagcacaa aataaaaaag cgcatcacga ctatactatc gttgaaacaa
     4861 tagaagcagg aattgttttg acaggaacag aaatcaaaag tgtccgcgca gcgcgtatta
     4921 cattgaaaga tggatatgca caaataaaaa atggtgaagc gtggttgatt aatgttcata
     4981 ttacaccata tgatcaaggt aacatatgga atcaagatcc tgatcgtaca cgaaagttat
     5041 tactaaaaaa acgtgagatt gaaaaaatat caaatgaact gaaagggact ggtatgactc
     5101 tggtacctct aaaagtttac ttaaaagatg gttttgctaa agttctattg ggtttggcaa
     5161 aaggaaaaca tgattatgat aagcgagagt ctatcaaacg tcgtgagcaa aaccgtgata
     5221 ttgcccgtca gttaaaaaac tataattcac gatgattatc aaaaggttag gcaattgcct
     5281 aacctttttt ggacgaaaaa agtgaagttg attgaccaac ttcactaata atatagactt
     5341 aatgtgattt atgtttgaag tgactatata aaccaaatcc aataatccag atggttgctc
     5401 caatagcccc gatgtatgtt gaatcttgca agaagagaga aacgaataca aagaggaaga
     5461 agagaagtgt gattggtgaa aggattttgt aggcaggcat tttataacca tcttccataa
     5521 aatcaggtga tttacgatat ttccaatgtg caatcataat aagaacatag atagaaatgt
     5581 aaacaccaga agatgatgca gtaattaagg agaaggcatc tgaaatacca ggtaaaacac
     5641 tgataagtgc tgaagctcca acgattacag ctgatgcaat aatagctcga cttggaacgc
     5701 tttgacgaga taattggtct aattttaacg cttttgtaag agcatttggt gattcattag
     5761 ccaattggaa taaatgacgc cctgttgagt ataaagtaga attaagagca gatgctgcag
     5821 acgttaggac aacaaagtta atcaaagcgg cagcccactt aatcccagct agtttgaata
     5881 ctgtaacgaa aggtgactca ttaactggta actgttgcca tgggaagatg gccataattg
     5941 ctaataatga acctgcgtag aataaaataa tacgcatcgg aatttcttgg attgcttttg
     6001 gtaaaacttt acgtggatta gctgtttcag aagttgttac tccgacaaat tcaatagcta
     6061 aataagcaaa gaataccatt tggaaagcca tgaagaaatt caatttacct ttagggaacc
     6121 attcaaaatg attggtgata tttgagattg aagcatggta tccgtgaccg gtgtcaaagt
     6181 ttgttaatac cataaagata ccggttgcta ttaaaccaag aattgcaata acttttatca
     6241 tagcaaacca aaactcggtt tctccgaatg cttttacagc aattaagttg atagaactta
     6301 ggatagctaa aaaaacaatt tggataatcc aggatggcca ttcagggaac caaaattgaa
     6361 catactctcc tacggctgta atctctgcca taccgataaa tattagggaa atccaataag
     6421 aaagtgttga gaaaaatccc caaccgggtc ctaaatattt ggtaatgaaa ttaataaaag
     6481 tatgctgatt tggatcttga taaagcatct ctccaatagc acgcatcatg aagaacatga
     6541 aagcgccagt gatagcatag acaagaacta ttgaaggacc cgtcaggcta attgaacgac
     6601 ctgcaccaag aaaaagccca gttccgattg tccctgcaat cgcaattaac tgtacgtgac
     6661 gattttctag gccgcgtacc ataccattct cagatttttg agtgtggtca ttattatttt
     6721 ttgacattat aaatactcca tctatttttg aaaaactcct ttattatata atttttttgc
     6781 aaaattcgca ataattacta tacaatattt ttctatgata aaatctccta gctaagtgag
     6841 ctaagagatt tcaattcatc cctcttcctt gacaatatag actgggcgtt ttttagtttc
     6901 taggaaaatc ttagagatgt atttgcctat aattcctagc gacaataact gaataccacc
     6961 cataaaaagc acaatggtaa ctgtgcttgc ccaacctgaa acgggatccc caaatagtaa
     7021 ttttcgaata ataataaaaa tgatagctaa aagtgatagt actgaactaa gggttccaat
     7081 ccaggtagct atagctaatg gaatttctga gaagttgatg aaaccgtcta gagagtattt
     7141 taagagatta aagaaactcc atgaggtctt acccgcaatt cgttctcgat tctcataact
     7201 aatataagcg acatcatatc caatccagga aaaaatccct tttgagaagc gattcacttc
     7261 atttagctcg agaatactgt caacaacttg ttttgtcatg agacgaaaat cacgcgcacc
     7321 atcaaccatt ttagtatctg acacttgatt gattaattta taaaaaagct tggcaaaaag
     7381 agagcgtatc aacggctccc ctttacgatc ttttcgcctt gtagcgacaa tatcataacc
     7441 ttcttgaagt tttaggtaca tttctggtaa taaagtgggt ggatcttgca agtcaacatc
     7501 cataacagtg ataaaatcac cagtagtatg ttctaaacca gccaataaag cagcatcttt
     7561 accaaaatgg cgtgaaaatg aaaggtaatg aacattgtca aattgtttag ctgctttctt
     7621 aagaagttcg agtgtacgat cggtagaacc atcgtttacg aaaatgtatt caaaatgggt
     7681 gtaacgagcc atttcggatt caactttttt gatttctgtt aaaaaagttg agacagtctc
     7741 ttgttcatta taacaaggga caataactga taataaagcc atttgtaatt ctccgtatca
     7801 ttcttgctta aattttaaca taatatggga aaaaatagaa agattataag gtaatcataa
     7861 aaaggactag gtttttgcct cagtccttaa cttaatatga tgagatgtta ttagtcagct
     7921 ttaaattgac tgagtcgaat aacttcaatt ttatagccat ctggatcttg aataaagtag
     7981 aacatattag gtttacctgg taatccagaa atttttgtta cagagtaacc tgctttctga
     8041 tgggcatcat aagtagtttc caaatcgtct acaccaacag caatgtgacc atacccattt
     8101 cctaaatcat aagcttcgtg atcatagtta taggttaact ctaattcgta actagggtcg
     8161 tcctctaggg ccatatagac taaagtaaat tgattttcag gaaagtcatt acgacgaact
     8221 tccttaaaac caagtgcttc ttgataaaaa gcaattgagg cgtccaaatc tttaacgcga
     8281 atacaagtat gtaaaaatgg catagtctct ctcctatttc tttattaaac gtattatgtc
     8341 aaaataaaat tgtcttttca aaaatattga ttataaatta ggctgtctat ttactcaacc
     8401 caagtaacga cctcttgacg ttctttccta gcacgggaat gtttaggttc acgcaaacga
     8461 taaccaaatg aaaccataca agaaatagct tctttcttat cgtctattaa cccttcttta
     8521 ctaagaatat tattgacctt ttcatagtca aatccttcaa ttgggcatga atctacgccg
     8581 atcatagcag cagccgtcat catattacca agtgcgatat aagtttgttt tgctgtccaa
     8641 tcccaaagag aacgttcaga atcaattttc atatcgtttt cttggaagga tttgtagaga
     8701 ggtatcctac tctcaagggc ggctggatca cctagaccac gtcgaatgag actgttaatc
     8761 atgctatcag cgttataata agcacctttt tcagctagta gtaaaacaaa gtgactagca
     8821 gtatctagtt gatattgtgc tccccaagca acttccttaa gtttatctcg aaattttgca
     8881 attgtttgtc tatctagtac taaaaatcgc cagccttcta aaccgactga agaaggactc
     8941 aaccaagcag tgtctaaaat atactccata tcttctttag gaatatcatt attattataa
     9001 acacgaatcg ccatacgaaa atcaaaagct cttcgaactt gttttttgat atcttctttt
     9061 gtcattttag atcctcctgt tattaatgct atcatatcac aatttagaaa aaatctcagc
     9121 aggcggtatc gtggcaaagt agagctacaa aattctaatg actgtcagaa aaataaaggg
     9181 aatgaacaaa atgatagggt tttgatgaag aaagttatat aattttacat aaggagggga
     9241 taataccata tatcaaacta tcatttagat gtttaaaata gatgtttttc actatgaaat
     9301 tcatttttgt cacatagaaa aggagattgt tatggaaaca tatactctat cgaatactct
     9361 taacattcct aaaattggtt ttgggacatg gcaactaact gaaggtgaag aagcttataa
     9421 agcagtgact catgctttaa aagttggtta tactcatatt gatactgctc agatttatgg
     9481 taatgaacat agtgttggac gtgctataag agacagtggt ttggcgcgag aaagtatctt
     9541 tttgacaact aagatttgga atgacaaaca tgactatcat ttagccaaag cttctattga
     9601 tgaatctcta cagaaattag gtgttgatta tattgatctt cttttgattc attggccaaa
     9661 tccaaaagct cttcgtgaaa atgatgcgtg gaaggctggg aatgcaggca cttggaaagc
     9721 gatggaagaa gcttataaag aaggaaaggt aaaagctatt ggtgtttcga atttcatgaa
     9781 acatcactta gaggctttat ttgaaaccgc cgaaataaaa cctatggtta atcaaattat
     9841 cctagcacca ggttgtgctc aagaagattt ggttcgcttc tgtaaaggaa atgatatctt
     9901 attagaagct tacagtccat ttggaacagg tgctattttt gaaaatgaat ctataaaagc
     9961 tattgctgaa aaatatggta aatcagttgc acaagtagct cttcgctggt cacttgataa
    10021 tggcttttta ccattaccta agtcagcgac tcccaaaaat attgaagcaa accttgatat
    10081 ttttgatttt caattaaacg aagatgatat tactacctta atacaactag attcaggtat
    10141 taaacccaaa gatccagata atgtctcatt ttaataagaa aagatggaaa tgtatttcca
    10201 tcttttttat gtgggatagt tccttaaagt taagaaaacc attaagacct ccattgacga
    10261 ggagaatata ttaatgatag tattactaga aaagtagtaa taaaggatga atatggtaaa
    10321 atcctttaca atatatcatt atcgaaagag gttataacat ctaaatgaga cttgataaat
    10381 ttttagtaga atgcgggctt ggttcacgta ctcaagtcaa attaatattg aaaaaaaagc
    10441 agattagtgt taatggtaat agcgagacat ctccaaaagt acaggtagat gaatatagag
    10501 atgagattaa atataatgga actctagtgt catatgaaaa gtttgtttat tatatgcttc
    10561 ataaacccaa aggtgttata tcagcaacgg atgatccttc gcataaaacc gtgttagatt
    10621 tgctagacaa aactgctcga gataaagctg tttttccagt ggggcgttta gatattgata
    10681 caacaggcct tttgttaata actaataatg gggaattagc gcataaaatg ttatcaccta
    10741 aaaaacacgt tgataaatgc tatgaggcaa aaatctcagg gataatgaca gaagacgata
    10801 ttttggcatt tgataaaggg attattctga aagattttac ttgtttacca gcattattag
    10861 aaattgtgga agtaaatcag gtgaagaagc agagtcttgt taaaatcact attaaggaag
    10921 gaaaattcca tcaagttaaa cgtatggttg ctgcgtgtgg taaggaagtc ttagagctaa
    10981 agcgtctaag tatggggaat ctacaattag ataaacagct agaaagtgga caatggagac
    11041 ggctaacaat aaaagaaata gaaaaattag aaaaatatat gcaataatat aaaataacag
    11101 ttaatatttt agcaatttga taacacttag attataatgt aaacgtatac ttttttagat
    11161 tggaagatca aatgaaacgt aaatacttta ttcttaatac ggtgacggtt ttaacgttag
    11221 ctgctgcaat gaatactagc agtatctatg ctaatagtac tgagacaagt gcttcagtag
    11281 ctcctactac aaatactatc gttcaaacta atgacagtaa tcctaccgca aaattttcat
    11341 cagaatcagg acaatctgta ataggtcaag taaaaccagc taattctgtg gcgcttacaa
    11401 cagttgacac gcctcatatt tcagctccag atgctttaaa aacaactcaa tcaagtcctg
    11461 tcgttgagag tccttctact aagttaactg aagagacata caaacaaaaa gatggtcaag
    11521 atttagccaa catggtgaga agtggtcaag ttactagtga ggaactcgtc aatatggcat
    11581 acgatattat tgctaaagaa aacccatctt taaatgcagt cattactact agacgccaag
    11641 aagctattga agcggctaga aaacttaaag ataccaatca gccgttttta ggtgttccct
    11701 tgttagtcaa ggggttaggg cacagtatta aaggtggtga aaccaataat ggcttgatct
    11761 atgcaggtgg aaaaattagc acatttgaca gtagctatgt caaaaaatat aaagatttag
    11821 gatttattat tttaggacaa acgaactttc cagagtatgg atggcgcaat ataacagatt
    11881 ctaaattata cggtccaacg cataaccctt ggaatcttgc tcataatgct ggtggctctt
    11941 ctggtggaag tgcagcagtt attgctagcg ggatgacgcc aattgctagc ggtagtgatg
    12001 ctggtggttc tatccgtatt ccatcttctt ggacgggctt ggtaggttta aaaccaacaa
    12061 gaggattggt gagtaatgaa aagccagatt cgtatagtac agcagttcat tttccattaa
    12121 ctaagtcatc tagagacgca gaaacattat taacttatct aaagaaaagc gatcaaacgc
    12181 tagtatcagt taatgattta aaatctttac caattgctta tactttgaaa tcaccaatgg
    12241 gaacagaagt tagtcaagat gctaaaaatg ctattatgga caacgtcata ttcttaagaa
    12301 aacaaggatt caaagtgaca gagatagact taccaattga tggtagagca ttaatgcgtg
    12361 attattcaac cttggctatt ggtatgggag gagctttttc aacaattgaa aaagacttaa
    12421 aaaaacatgg ttttactaaa gaagacgttg atcccattac ttgggcagtt catgttattt
    12481 atcaaaattc agataaggct gaacttaaga aatctattat ggaagcccaa aaacatatgg
    12541 atgattatcg taaggcaatg gagaagcttc acaagcaatt tcctattttc ttatcgccaa
    12601 cgaccgcaag tttagcccct ctaaatacag atccatatgt aacagaggaa gataaaagag
    12661 cgatttataa tatggaaaac ttgagccaag aagaaagaat tgctctcttt aatcgccagt
    12721 gggagcctat gttgcgtaga acacctttta cacaaattgc taatatgaca ggactcccag
    12781 ctatcagtat cccgacttac ttatctgagt ctggtttacc catagggacg atgttaatgg
    12841 caggtgcaaa ctatgatatg gtattaatta aatttgcaac tttctttgaa aaatatcatg
    12901 gttttaatgt taaatggcaa agaataatag ataaagaagt gaaaccatct actggcctaa
    12961 tacagcctac taactccctc tttaaagctc attcatcatt agtaaattta gaagaaaatt
    13021 cacaagttac tcaagtatct atctctaaaa aatggatgaa atcgtctgtt aaaaataaac
    13081 catccgtaat ggcatatcaa aaagcacttc ctaaaacagg tgatacagaa tcaagtctat
    13141 ctccagtttt agtagtaccc cttttattag cttgttttag ctttgtaaca aaaaagaatc
    13201 agaaaagttg attgtaatgt aaaataattc ctatttttca ctttttagta tcatattttc
    13261 gatttttatg ttgtaataaa ttataatgaa ggtgtaagga attataaaat aggaaataat
    13321 atgaaagtga aaaataagat tttaacgatg gtagcactta ctgtcttaac atgtgctact
    13381 tattcatcaa tcggttatgc tgatacaagt gataagaata ctgacacgag tgtcgtgact
    13441 acgaccttat ctgaggagaa aagattagat gaactagacc agtctagtac tggttcttct
    13501 tctgaaaatg aatcgagttc atcaagtgaa ccagaaacaa atccctcaac taatccacct
    13561 acaacagaac catcgcaacc ctcacctagt gaagagaaca agcctgatgg tagcacgaag
    13621 acagaaattg gcaataataa ggatatttct agtggaacaa aagtattaat ttcagaagat
    13681 agtattaaga attttagtaa agcaagtagt gatcaagaag aagtggatcg cgatgaatca
    13741 tcatcttcaa aagcaaatga tgagaaaaaa ggccacagta agcctaaaaa ggaacttcct
    13801 aaaacaggag atagccactc agatactgta atagcatcta cgggagggat tattctgtta
    13861 tcattaagtc tttacaataa gaaaatgaaa ctttattaaa tataaaaaag gaggactaac
    13921 ctccttttta tatttaaata gcccattcgc catttctaaa gattgggaca gcagttccat
    13981 ctgcacgaat accatcaata tccatttgtt ctgaaccaat cataaaatca acatgtgttg
    14041 aagaacgatt taaaccagct tcatccaatt cttgacttgt catttctgtt cctccttcta
    14101 cattgaaggc atatgcagat ccaatagcga ggtgattaga ggcattttca tcgaataaag
    14161 tattaaagaa aattagtcca gacagtgaaa ttggtgtttt atgtgggaca agtgcaactt
    14221 ccccaagcga tcttgcccca tcattttcct cgattaagcg ttggattgtt tcttgccctt
    14281 tttctgcagt gacattgata attttaccgt ctttaaaagt aaacatcata ttttcgataa
    14341 taacgccagc ataactgaga ggttttgtac ttgtcacata cccatctgca cgacggtaat
    14401 caggtgctga aaagatttct tcagtaggca tattagcgat aaaagtctct ccctgagcgt
    14461 tgagactacc ggctgcctcc caaatatgat ttttaggcat accaagcgtt aaatctgtac
    14521 ctggtgcggt ataatgaaga gcatcaaatt gttcttgatt tagtctagta gctttttcta
    14581 ataatttagc ttcatgtgcg tcccaagcag caataggatc atcttcataa atacgattta
    14641 atttgaaaat ggtatcccaa agagcatcaa cttgttggtc gcttgttttt aattctggga
    14701 agacaagtgc agcccattct ctaccagcag ctgcagccac gttccaagat actttattag
    14761 cttgtattgc tctactttgt tcctctaagg caatagcggt tgctctagtt gtttcagata
    14821 aacgtttagg atcaacagta gcgaaagcgt tagggttaga agatttcaca actaatcgac
    14881 tagctttctt atttaatagc gctaaagatt tatcaacggt atactgtgga acattcgtga
    14941 gaacttcgtc tgaagcatga agtagtcgct ggcgcgtgat aaaatcatct gtataatcaa
    15001 caataacttc acttgctccc ttttcataag cggcttcagt taaaagcctt gctaagtgga
    15061 cttgttccac atcgattgtt aaagcgagag tatgcccttt ttggacattt aaaccttgag
    15121 aaataattaa ttgggcatat ttttttaaaa ggttgtcgaa atcttgtaaa accatactag
    15181 ctcctttaaa tttactattt tttataattt atcataaact cttataaagc acaaggaaat
    15241 atggaagcga tttaataaag atatatttaa gctaacggtt tgtagtttgc gaaataagac
    15301 gtttcgtgtt aatatagact taactatata ttcagtaaat gaggtgacat gagtggcttt
    15361 tggagataac ggacaacgaa aaaaaactgg gtttgaaaaa ctaactcttt ttgttgttat
    15421 tttgatggta ctagtaacag ttggtggttt ggtatttggt gctattagtg caatcatgta
    15481 atgagaaacg ctgtaaagcg tttttttata ctgaatagaa taaagaatag gaattttaga
    15541 aatgagtatg tttttagata ctgccaaaat tagtgttaag gcaggtcgcg gtggtgatgg
    15601 catggttgct tttcgccgtg agaaatatgt ccccaatgga ggcccgtggg gcggtgatgg
    15661 cggtaaaggc ggatcggtca tttttaaagt aaacgaaggt ttacgaactt tgatggattt
    15721 tcgatataac cgtaatttta aagcaaaagc tggtgaaaaa gggatgacta aaggtatgca
    15781 cggtcgaggt gcagaagacc ttattgtgtc attaccacct ggaacaactg ttcgtgatgc
    15841 tactacaggt aaggttatta ccgaccttgt ggagcatgat caggaattcg ttgtagccag
    15901 aggtggccgg ggtggtcgtg gaaatattcg ctttgcaacc ccacgtaatc cagctcctga
    15961 aattgctgaa aatggggagc ctggcgaaga gcgtgaatta caattagaat taaaaatttt
    16021 ggctgatgtt ggtctcgttg gtttcccatc ggttgggaag tcgacgctac ttagtgttgt
    16081 ttcagcagca aagcctaaaa ttggtgccta tcattttacg acaattgtcc ctaatttagg
    16141 gatggttcgt acaaaatcag gtgatagctt tgcaatggct gatctccctg gtttgattga
    16201 aggggctagt caaggtgtcg gattaggaac tcagtttctt agacatattg agcgtacacg
    16261 tgttattcta catgttattg atatgtcggc aagtgaagga cgagaccctt acgatgacta
    16321 tgtttcaatt aataatgagt tggaaactta caatcttcga ttgatggaac gtccgcaaat
    16381 tattgttgcc aataaaatgg acatgcctga ttcagaagag aatttggcag cattcaaaga
    16441 aaaattagct gcaaactatg atgagtttga tgacatgcct atgatttttc caatttcaag
    16501 tttagcacat caaggactag agaaccttat ggatgcgacg gctgaattat tagccaatac
    16561 agaagagttt cttctttatg acgaaactga catgcaagaa gatgaagcat actatggatt
    16621 taacgaagat gaacgtccat ttgagattac acgtgatgat gatgcaacgt gggtattgta
    16681 tggtgataag ctagaaaaac tctttgttat gacaaatatg gaacgcgatg aatcaataat
    16741 gaaatttgct cgtcagttac gtggtatggg cgttgatgaa gcccttcgag agcgcggagc
    16801 taaggatgga gatattgttc gtataggtaa cttcgaattt gaatttgtag attaatgcag
    16861 tacttgattg gaggtttttt atgggagata aaccgatatc atttcgtgat aaagatggga
    16921 actttgtttc agcggccgat gtctggaatg ctgaaaaatt agaagaactc tttaacacat
    16981 taaatcctaa tcgtaaactt cgtttagaac gcgaaaaatt agcaaaagaa aaaaggatag
    17041 ttaagtaact atccttttta gttattttag gtatgaaaaa agactctaaa acataattga
    17101 tagagtgtta aggtacagat tactttatta ttttaaacgt ttttgagctt tttgatatac
    17161 tgaagagcat tttttacaat ggttggagta taagtaataa atttataagc aaaataaaaa
    17221 gcacaaacta aagttataaa aaatagtaag taaataatag ggttttgcgt taagaatcct
    17281 tgaaaaagaa gtacaaggtt aagaattacg gtagagatac ttaagataaa aataactaga
    17341 taagcaataa tagttgtcca aaaccttaaa tggatgttac tcaccttttc ccatttaata
    17401 tctaaaaagt catctataaa gttgacatga taagtcatat gttctttaat aaagtgcaat
    17461 attctgttaa tatagtcttt catactgcta tattatcata ctgaaagccc ttgtgcaaca
    17521 ttattcaaac attctagctg atgttttgct aattttagaa gaaaacttat cctattataa
    17581 tttgtttgtg attaggaaat aaaaaaagat gacacgaagt catctttttt attaaacgtt
    17641 taaaacttta ttaaggaagt cttggagacg tgggtgttga ggtgtatcaa agatttgctc
    17701 aggtgtccca tcttcgagga aacgaccacc atctgtaaag ataacacgat tggcaacttg
    17761 acgagcaaat cccatttcgt gtgtcacaat taacatagtc ataccttgtt cagctaaatc
    17821 tttcataaca ttgagaacat ctccaaccat ttctggatca agtgcagaag ttggttcgtc
    17881 aaaaagtaag atatctggat tcatcgctaa gcttcttgcg atagctacac gttgtttttg
    17941 tcctccagat aaactatctg gtttggcatt agctttatcg gctaaaccaa ctttttcaag
    18001 taattccatc ccatgctttt cagctgcttc tttagattct ttaccaagtt caataggagc
    18061 aaaagtgatg ttttcaagaa ctgacatatg tgggaaaaga ttgaagtgtt gaaataccat
    18121 tccgatattt tcacgtgctt tgtctatgtc tgtttttgga tttgataact caaagccgtc
    18181 cacaacaact tttccacttg tgattgattc aagtaagttc aatgtgcgaa ggaaagttga
    18241 ttttcctgaa ccagaaggtc caataataca gacaacatca ccttcataaa atttagcatc
    18301 aatacctttt aaaacttcat tttgcccata cgacttgtgt aaatcttgga catcaatttt
    18361 taattctgcc attatttaag cctcttttct aaacgttttg ctagacgggt gagaagggta
    18421 atgattacaa gatacataat tgcaagaata gcatacatac ggaatgattg atagttacgt
    18481 gcaattatga ttttaccagt ttggaagagt tccacaagtc cgattgctga tacaattgtt
    18541 gtatccttta atgagatgac aaattggttg ataaagtttg gtaacataag gcgtactgct
    18601 tgaggcaaga taaccttttg catagttttg ccgtaagaaa tacctaaact acgacttgct
    18661 tccatttgac cagaaggaac agcttcaata ccaccacgta caatttcagc aatgtacgca
    18721 ccaccattta aagaaagagc gatagtagca gcaacgaagt cattaattgg actttggtga
    18781 cctgtgatgc tttcgattaa attaggaata ccccagaaaa taaaagcggc cacaatcatg
    18841 agtggaatac cacggacaac atcaacaaaa atcattgaaa ttgtgcggag agtattactt
    18901 ggtgatacgc tcatcatacc aaagataata ccaataacca tagcaatcgc aaatgagata
    18961 agagttaaac ttaaagtagt tccaatacca gatagcaatt gtttgtagtt attagaaatt
    19021 aaccctaaaa tagttgattc atctacaggt ttagcagctt tatcgtttga agaagtgctg
    19081 gctgtggaaa ggtatttttt aacaagttta tcgtactcac ctgatttttt gagtgaagca
    19141 agaccgttgt taaacatttt aattaattct ggatttgccc cttttttcac tgcaaatccg
    19201 atatcgcctg atttttcacc tttgataggt gtttcaaatt tacgaccttg attaatagcg
    19261 taagcaagaa ctgcttcgtc atccattaga gcatcaattg aacctgagtt catactatca
    19321 tacattgtag atgcttcatc aaatgcttta acatgatagt tgtacttatc tgcgtggtct
    19381 gataaccaag tatatgaggc agtaccattt ttagcaccaa ctgtttttcc ttttaaatct
    19441 tggtatgatt tgacattgct tccttttttt accgctaaga taacgctaga tgtgtaataa
    19501 gggtcagaaa aatcaaagat tttttggcgt gcttctgtga ttgtggctcc tgcaataaca
    19561 ccgtcagctt gtccagattg gacagcattt aaagcggcat caaaacctgg atttgagata
    19621 tcaagtttaa aaccttgctg tttagcaatt ttcttgatta attccatatc aaaaccagta
    19681 tatttccctt taccgttttg atattcgaat ggtgcgaatg aagaatcaga aacaatttta
    19741 taacttggct ttacaggagt tgctttttca ttggcattac ctgttgcttt gccgcttgtt
    19801 tttgtagcgt ctttcccaag ccatttatcc atgatgtcat tataagtgcc atctgatttc
    19861 atttgtgcaa aagctgtgtt aaattcttta attaggttat cgtgtccact accttttttg
    19921 acagcaaatg caaagctacc aactgcctcg ccttccatgt taatggcgta agcttttcct
    19981 tgatttatcg caaattgcac aactggttga tcgtccatag cggcgtaaat agaaccagaa
    20041 tcaaggctgt tattcattag gtcacttgta tcaaatgttt taactttata gccgtattta
    20101 gatttatttt cctctaagaa gctttgagca gctgttccat tttttacacc gactactttt
    20161 ccttttagtt gtttgtagtt tgtgacttta ttattatttt tagtataaag aataacggaa
    20221 gtatcgtaat aagtatctga gaaattaaag acttttttac gtgcttcagt aacagtagtt
    20281 ccggccatta gcgcgtctgc ctgtccagat tgaacagcgt taactgcggc atcaaaacct
    20341 gggtacgtca tgttaacatt ccagccagca cgcttagcga cttcgttaac gatgtcaaca
    20401 tcgattcctt tataagtttg atcagaatct ttaaattcga atggagcgta tgctgtatca
    20461 gaaacaatat tcacagtctc tgcactgacc tttccaccaa acacaaagaa tattgagagt
    20521 aaagcaagcg taaaagcttt taacttgtgc ttcatgtaaa tctccttttt ataaatatgt
    20581 gttttattat atcaaaataa ataagataga gcaacaaaat gtcatttcta tgttagaaaa
    20641 cgtaacatgt atgttatgtt aattttatat atatctttag ttagtattat gatataatcg
    20701 ttctagtgtt atcacacaaa aaggatttag
//
LOCUS       DATRNK010000033        20550 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.033, whole genome shotgun sequence.
ACCESSION   DATRNK010000033 DATRNK010000000
VERSION     DATRNK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20550
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.033"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..221)
                     /locus_tag="VBC52_001201"
     CDS             complement(<1..221)
                     /locus_tag="VBC52_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEO6882791.1"
                     /translation="MKVKLFAIHLFFIVISFGIKYYHLQGPNLIWNMTLALIALDFAY
                     LTSLFKKKILIGLFALAWFFFYPNTFYMLT"
     gene            complement(311..895)
                     /locus_tag="VBC52_001202"
     CDS             complement(311..895)
                     /locus_tag="VBC52_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882792.1"
                     /translation="MKKLIWSHLFAIVSILGIWYYHVKIDHLMVILLASVLSLEMGFI
                     AYYSRNIFIRLLASLGLLFFYPQSLGIFLSLVSLEWSTKDIWQSNHFMQYMAYLLAIL
                     FIISSALLSLKLLVNAYDISKRYQLATVIPVMLFSSFAFCLEKSGSLGLEGIILNPSA
                     FFNGIQTNMANLDFKALSCLFLVQLLLWGILMED"
     gene            984..1796
                     /locus_tag="VBC52_001203"
     CDS             984..1796
                     /locus_tag="VBC52_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6882793.1"
                     /translation="MTKKIIAVDLDGTLLHNNNTISDYTADTLRKVQAQGHKVIITTG
                     RPYRMALAHYLRLDLKTPMINFNGALTHIPEKKWAFERSATIDKKLLLETLNLSDAIQ
                     ADFIASEYRKNFYITMDNRDKINPQLFGVNEITDKMALDVTKITRNPNALLMQTRHKD
                     KYELAKELRQHFNHELEVDSWGGPLNILEFSPKGVNKAYALKHLLKSLNFSQENLIAF
                     GDEHNDTEMLAFAHTGYAMKNANPTLLPYADQQIQWTNEEDGVAKTLEKLLL"
     gene            2098..3108
                     /gene="manX"
                     /locus_tag="VBC52_001204"
     CDS             2098..3108
                     /gene="manX"
                     /locus_tag="VBC52_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEO6882794.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIAQFDADDEVLVLADLWSGSPFNQASRVMGENPERKMAIITGLNLPMLIQ
                     AYTERMMDANAGVEQVAANIIKESKEGIKALPEELNPVVEATPVAGVPADVPAEVKQS
                     GSIPEGTVIGDGKLKINLARIDTRLLHGQVATAWTPASKANRIIVASDEVSKDELRKQ
                     LIKQAAPGGVKANVVPISKLIEVAKDPRFGNTRALILFETVQDALRAIEGGVEIPELN
                     VGSMAHSTGKTMVNNVLSMDKDDVAAFEKLRDLGVSFDVRKVPNDAKKNLFDLINKAN
                     VK"
     gene            3141..3953
                     /locus_tag="VBC52_001205"
     CDS             3141..3953
                     /locus_tag="VBC52_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEO6882795.1"
                     /translation="MADISIISMILVVIVAFFAGLEGILDQFQIHQPLVACTLIGLVT
                     GHLEAGIILGGSLQMIALAWANIGAAVAPDAALASVAAAIILVKSGDFTQKGINFAFS
                     TAVPLAIAGLFLTMIVRTISTALVHAGDKAASEGNFAAIERFHFIALLLQGLRIAFPA
                     ALLLAIPSSSVQSILEAMPDWLNGGMQVGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAALNQLTLIAMGTIGVAIALIYISLSKMGGSKGTSNAGSNDPIGDILEDY"
     gene            3968..4879
                     /locus_tag="VBC52_001206"
     CDS             3968..4879
                     /locus_tag="VBC52_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6882796.1"
                     /translation="MTEQIKLSKSDRQKVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTTKEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEEKASGTPVEDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASAGNILGPIIFFVGWNLIRMSFLWYTQE
                     LGYKSGKEITKDMSGGILQDITKGASILGMFILAVLVKRWVAINFTVDLPKKTLSEGA
                     YINFPKDHVSGQQLHDILGQVQSGLSLDKMQPQTLQGQLDSLIPGLAGLLLTFFCMWL
                     LKKKVSPITIIIGLFIVGILARLAGVM"
     gene            4998..5360
                     /locus_tag="VBC52_001207"
     CDS             4998..5360
                     /locus_tag="VBC52_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEO6882797.1"
                     /translation="MAQSLNKTVEFQTTGVSYLGMGNKVGKFLVGDQALEFYNDKNVN
                     DYIQIPWTSINQIGANVSRKKISRHFEVFTDQGKFLFASKDSGTILKHARRHIGDDKV
                     VKLPTLIQTILKIFKFKK"
     gene            complement(5398..6459)
                     /locus_tag="VBC52_001208"
     CDS             complement(5398..6459)
                     /locus_tag="VBC52_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO6882798.1"
                     /translation="MEKYTSIKQSGRHYGLDLLRIISMFMIVITHVLGKGGLRSSVEG
                     HADSYFIVTWIIQVLVYGAVNCYALISGYVGINSRYRYSKLLSIWAQVFFYTFTITAL
                     FAITGHEVTLLNWRDAFFPIVSGQYWYITAYFGLLVFMPVINNGLNALTDKQLKQLVL
                     LMFIIFSILPAVLNNRVPEFSLSKGFEMTWLLILYIIGAYLKRIDLNIFKTSYLLIIY
                     LLSLVATYAMKFSVGDIWYWYVSPTLTLGAVSLFILFARASIKPSGFLKKIIVVLAPS
                     TLGVYLCHLHPLIVKYFVRDFAETFVYESIYLYPFLILGAGILIYLLAFIVERIRLRI
                     FKAIHFSNFLRWFDDKFPY"
     gene            6756..8033
                     /gene="serS"
                     /locus_tag="VBC52_001209"
     CDS             6756..8033
                     /gene="serS"
                     /locus_tag="VBC52_001209"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEO6882799.1"
                     /translation="MLDLKRIRTDFDVVAKKLATRGVDQETLTTLKELDIKRRELLIK
                     AEEAKAQRNVASAAIAQAKRNKENADEQIAAMQTLSADIKAIDAELADVDANLQSMVT
                     VLPNTPADDVPLGADEDENVEVRRWGTPREFDFETKAHWDLGESLGILDWERGAKVTG
                     SRFLFYKGLGARLERAIYSFMLDEHAKEGYTEVIPPYMVNHDSMFGTGQYPKFKEDTF
                     ELADSPFVLIPTAEVPLTNYYRDEIIDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLNLPYRVITLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEVDGKVRLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGNIDIIKPN"
     gene            complement(8079..8711)
                     /locus_tag="VBC52_001210"
     CDS             complement(8079..8711)
                     /locus_tag="VBC52_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEO6882800.1"
                     /translation="MYQELNDYLEFQSMKYIMPEKAGPLKDDMQTFKAKGQLARNSFT
                     ELSRALEQRMDGFKMQRVSNWANQAQVGRPHFWVYYRKDTDQLDDVAVALRVYGVKDS
                     FGVSLEVSFVERQKSDKTLEKQARVLSIPIASPLYFMVQRQGETHREEGNEENRQRLM
                     QEIKSGKVRKVLVKYDVLLTENQSLENILQRLLEGFEKVLPYYQVCQTIS"
     gene            complement(9181..9954)
                     /locus_tag="VBC52_001211"
     CDS             complement(9181..9954)
                     /locus_tag="VBC52_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEO6882801.1"
                     /translation="MNNDVTRILKDARDQGRLTALDYAELIFDNFMELHGDRQFADDK
                     SIIGGLGYLAGRPVTIVGIQKGKNLQDNLDRHFGQPHPEGYRKALRLMKQAEKFGRPV
                     ITFINTAGAYPGVGAEERGQGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALAVAD
                     KVWMLEHTVYSILSPEGFASILWKDGTRTTEAAQLMKMTAGELYHMEVVDKVIPEHGY
                     FSSEIVDMIKTSLISELEVLSQLSLEDLLEQRYQRFRKY"
     gene            complement(9947..10822)
                     /locus_tag="VBC52_001212"
     CDS             complement(9947..10822)
                     /locus_tag="VBC52_001212"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEO6882802.1"
                     /translation="MALFSKKDKYIRISPNKALGSSDKRSLPEVPDELFAKCPSCKHM
                     IYQKDLGLAKICPACSYNFRISAQERLLLTVDEDSFEELFTGIETKDPLNFPNYREKL
                     AATRQKTNLDEAVVTGLAKIKGQTTALAIMDSHFIMASMGTVVGEKLTRLFELATEKK
                     LPIVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNQGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQALVGFAGRRVIETTVREDLPEGFQKAEFLLEHGFVDAIINRTELRDC
                     IAQLIAFHGNEVGHE"
     gene            complement(10831..12201)
                     /gene="accC"
                     /locus_tag="VBC52_001213"
     CDS             complement(10831..12201)
                     /gene="accC"
                     /locus_tag="VBC52_001213"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEO6882803.1"
                     /translation="MFKKILIANRGEIAVRIIRAAREMGISTVAIYSEADKESLHTIL
                     ADEAICVGPAKSAESYLNVNAILSAAIVTGAEAVHPGFGFLSENSKFATMCEEMNLKF
                     IGPSGEVMDKMGDKINARTEMIKADVPVIPGSDGQVTSVEEAVSIAEEIGYPLMLKAS
                     AGGGGKGIRKVKSADELKPAFESASQEALAAFGNGAMYIEKVIYPARHIEVQILGDSF
                     GKIVHLGERDCSLQRNNQKVLEESPSVAIGNTLRQQIGEAAVRAAEAVSYENAGTIEF
                     LLDENSGQFYFMEMNTRVQVEHPVTEFVTGVDIVKEQIRIAAGIPLSVSQNDIKLTGH
                     AIECRINAENPQFNFAPCPGTINGLHLPAGGMGLRVDSAVYTGYTIPPYYDSMIAKVI
                     VHGENRFDALMKMQRALYELEIDGIVTNTEFQMDLISDKKVLAGDYDTSFLMEDFLPR
                     YYGNNQ"
     gene            complement(12239..12661)
                     /gene="fabZ"
                     /locus_tag="VBC52_001214"
     CDS             complement(12239..12661)
                     /gene="fabZ"
                     /locus_tag="VBC52_001214"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO6882804.1"
                     /translation="MIDIKEIREALPHRYPMLLVDRVLEVSEDEIVAIKNVSINEPFF
                     NGHFPEYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTAKFVKRRGTIAVVEAIAEVDGKLAASGTLTFAIGN"
     gene            complement(12658..13194)
                     /locus_tag="VBC52_001215"
     CDS             complement(12658..13194)
                     /locus_tag="VBC52_001215"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEO6882805.1"
                     /translation="MQFWPSNAGRIKLDIQEIKDLMTQFDESSLREFSFKTSDGELSF
                     SKNEGKAPLVPTMSPMSHQPEATPTIATPVSNEAGEQTKQATEVVSEIPESTVTVAEG
                     DVVESPLVGVAYLASGPDKPNFVSVGDSVKKGQTLMIIEAMKVMNEVPAPHDGVVTEI
                     LVANEEVIEFGKGLVRIK"
     gene            complement(13160..14392)
                     /gene="fabF"
                     /locus_tag="VBC52_001216"
     CDS             complement(13160..14392)
                     /gene="fabF"
                     /locus_tag="VBC52_001216"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEO6882806.1"
                     /translation="MTLQRVVVTGYGVTSPIGNTPEEFWNSLKEGNVGIGPITKFDSS
                     DFMVKNAAEIHDFPFDKYFVKKDLNRFDMYSLYALYASSEAIQHANLNLDEIDADRFG
                     VIVASGIGGIQEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMRLGAHGVCKSI
                     NTACASSNDAIGDAFRNIKFGIQDIMVVGGAEAAITKFAIAGFQSLTALSTTEDPSRA
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAEKRRATILAEVVGYGNTCDAYHMTSPHPEG
                     LGATKAIQLALVEANIKPEEVNYVNAHGTSTPANEKGESQAIVAALGTDVPVSSTKSF
                     TGHLLGAAGAVEAIATIEAIRHSYVPMTAGTTELSEDITANVIFGQGQDADIRYAISN
                     TFGFGGHNAVLAFKRWED"
     gene            complement(14408..15142)
                     /gene="fabG"
                     /locus_tag="VBC52_001217"
     CDS             complement(14408..15142)
                     /gene="fabG"
                     /locus_tag="VBC52_001217"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEO6882807.1"
                     /translation="MQLKDKNIFITGSSRGIGLAIAHQFAQLGANIVLNGRSEISEDL
                     IAEFADYGVKVIAISGDVSSFEDANRMIKEAIASLGSVDVLVNNAGITNDKLMLKMTV
                     EDFESVLKINLTGAFNMTQSVLKPMTKARQGAIINISSVVGLTGNVGQANYAASKAGL
                     IGFTKSVAREVAARGIRVNAIAPGFIESDMTDVIPEKMQEAILAQIPMKRIGKGKEVA
                     QVASFLAEQEYLTGQVIAIDGGMTMQ"
     gene            complement(15151..16077)
                     /gene="fabD"
                     /locus_tag="VBC52_001218"
     CDS             complement(15151..16077)
                     /gene="fabD"
                     /locus_tag="VBC52_001218"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEO6882808.1"
                     /translation="MNKVAFLFAGQGAQKLGMARDLYETFPIVKETFDKASHVLGYDL
                     RELIDKDLDKLNQTKYTQPAILTTSTAIYRLILKEIELRPDMVAGLSLGEYSALVASG
                     AIRFEDAVVLVARRGQLMEAAAPAGSGKMVAVLNADRQIIEDACKKASQFGIVSPANY
                     NTPKQIVIGGESIAVNAAVEELKQQGVKRLIPLNVSGPFHTALLKPASQKLSDVLDKV
                     HFSVSEIPVIGNTEAQIMKKDDIKSLLARQVMEPVRFDESIETMKKMGMTQVVEIGPG
                     KVLSGFLKKIDSSLSVHSVEDKIGFNNLKELN"
     gene            complement(16097..17056)
                     /gene="fabK"
                     /locus_tag="VBC52_001219"
     CDS             complement(16097..17056)
                     /gene="fabK"
                     /locus_tag="VBC52_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEO6882809.1"
                     /translation="MKTRITELLNIKYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSMTDKPFGVNIMLLSPFVDDIVDLVIEEGVKVVTTGAGNPGKY
                     MERFHEAGITVIPVVPSVALAKRMEKLGADAIITEGMEAGGHIGKLTTMTLVRQVVDA
                     VTIPVIAAGGIADGRGAAAGFMLGADAVQVGTRFVVAKESNAHPNYKAKILKAKDIDT
                     AVSAQVVGHPVRALKNKLVTTYSQAEKDYLAGRISINEIEELGAGALRNAVVDGDVIN
                     GSVMAGQIAGLIKSEETCQEILEDIYSGARQVILSEASRWSDL"
     gene            complement(17211..17435)
                     /locus_tag="VBC52_001220"
     CDS             complement(17211..17435)
                     /locus_tag="VBC52_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO6882810.1"
                     /translation="MAVFEKVQEIIVEELGKDAEEVTLNTTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKVK"
     gene            complement(17493..18464)
                     /locus_tag="VBC52_001221"
     CDS             complement(17493..18464)
                     /locus_tag="VBC52_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEO6882811.1"
                     /translation="MVFAKISQLAHYAPSQIIKNEDLSLIMDTSDDWISSRTGIKQRH
                     ISKNETTADLANKVAEQLIEKSGYSASQIDFIIVATMTPDSMMPSTAARVQAHIGASN
                     AFAFDLSAACSGFVFALSTAEKLISSGSYQKGLVIGAETVSKVLDWTDRGTAVLFGDG
                     AGGVLLEASKEKHFLAESLNTDGSRQGLQSSQVGLNSPFSDEVLDDKFLKMDGRAIFD
                     FAIKEVSKSINHLIETSYLEKEDIDYLFLHQANRRILDKMSRKIDIARDKFPENMMDY
                     GNTSAASIPILLSESYENGLLKLDGNQTILLSGFGGGLTWGSLIVKI"
     gene            complement(18464..18898)
                     /locus_tag="VBC52_001222"
     CDS             complement(18464..18898)
                     /locus_tag="VBC52_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6882812.1"
                     /translation="MEYDQINSYLVDIFNRIMIIEEMSLKTSQFSDVSLKEMHTIEII
                     GKHSEVTPSDVARELMLTLGTVTTSLNKLEKKGYIERKRSSIDRRVVHLSLTKRGRLL
                     DRLHSKFHKSMVSHIIEDLGEEDIKMLTSALGNLHKFLEDLV"
     gene            complement(18994..19785)
                     /locus_tag="VBC52_001223"
     CDS             complement(18994..19785)
                     /locus_tag="VBC52_001223"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEO6882813.1"
                     /translation="MKFENIIYGIDGNVATIMLNRPDISNGFNIPMCQEIIDAIRLVS
                     ENKDVMFLVIEAQGPIFSIGGDLKVMKAAVESDDISSLTKIAELVNQISYDLLQLEKP
                     VVMCVDGAVAGAAANIALAADFVIASKKSKFIQAFVGVGLAPDAGGLLLLSKSIGITR
                     AVQLALTGESLSAEKAEALGIVYKLCESDKIGKIKDQLLKRLSRHSINSYQAIKSLAW
                     EAAFKDWEQYKKLELQLQESLAFKQDFKEGVRAHADRRRPNFLGK"
     gene            complement(19922..>20550)
                     /locus_tag="VBC52_001224"
     CDS             complement(19922..>20550)
                     /locus_tag="VBC52_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001900118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEO6882814.1"
                     /translation="GVLFDTELFYYKRRERFLKQHGITIDHLPMNFFIGGNMKQVWKS
                     VLGDQYDTWDIDKLQQDYSRYKEDNPLPYKDLIFQDCKRVIEKLHHKGYLLGLASSST
                     RHDIMLALESFNLDTYFKVILSGEEFSESKPNPAIYNRAAELLDIPKQQILIVEDSEK
                     GITAGIAAGIAAGIDVWAIEDKYFGMNQSQANVLVSDLSQFFVKLDNY"
BASE COUNT         6580 a         4220 c         3248 g         6502 t
ORIGIN      
        1 gtcaacatat aaaaggtatt aggatagaaa aaaaaccaag caagtgcaaa aagaccgata
       61 agaatttttt tcttaaataa actagtaagg tatgcaaaat ctaacgctat tagggctaga
      121 gtcatattcc aaatcaaatt tggtccctgc aaatgataat atttaatccc aaaagaaata
      181 acaataaaaa atagatgtat cgcaaatagt tttactttca ttctaacctc caattaatat
      241 ctcattatac aacatattat taaattaagg ttaaataaac ttcaaaccta aaagactaac
      301 cctgttgtgg ttagtcttcc attaatattc cccaaagtaa taattgtact aaaaaaaggc
      361 aagacaaggc tttaaaatca aggttagcca tattagtctg tattccatta aaaaatgctg
      421 acggatttaa aatgattcct tctaaaccta atgatcctga tttttctaag caaaaggcaa
      481 aactactaaa aagcattaca ggtataacag ttgctaattg atacctttta ctaatatcat
      541 atgcatttac caatagcttc aagcttagta aagctgaaga gataataaaa agaattgcaa
      601 gaaggtatgc catatattgc ataaagtgat tagactgcca aatgtctttt gttgaccact
      661 ctaagctcac caaactaaga aaaatcccta aactctgtgg gtaaaaaaat aaaagaccta
      721 aacttgctaa aagacgaata aaaatattac gactataata agcaatgaat cccatctcta
      781 acgacaagac tgatgccaat agaattacca tcaaatgatc aatttttaca tgataatacc
      841 atatccctaa aatactgact atggcaaaaa gatgactcca aattaacttt ttcataatcc
      901 accaacttcc atatttccac agttattata tcatatttct actgtctctt tttattgaaa
      961 tctatttttg atataatgtc agcatgacaa agaaaattat cgccgtagat ttagacggta
     1021 ctctattaca taataataac actatttctg actacaccgc tgatacactt cgaaaagttc
     1081 aagctcaagg acacaaagta attattacaa ccggacgccc ataccgtatg gcactcgctc
     1141 attatttacg cctagattta aaaacaccaa tgataaattt taatggtgct ttaactcata
     1201 tccccgaaaa aaagtgggcg tttgagcgta gtgcaacgat tgataaaaaa ttactccttg
     1261 aaaccttaaa tttatcagat gctatccaag ccgattttat tgctagcgaa taccgtaaaa
     1321 atttttacat tactatggat aatcgtgata aaattaaccc acaattattc ggtgtcaatg
     1381 aaattactga taaaatggca ttagatgtta ctaaaatcac acgcaatcca aatgctctat
     1441 tgatgcaaac acgtcataaa gataagtatg agttagctaa agagttacga caacatttta
     1501 atcacgaact agaagtagat tcttggggtg gtccattgaa tatactcgaa ttttcaccta
     1561 aaggtgttaa caaagcttat gctctcaaac atttattaaa atcactcaat ttttcacaag
     1621 aaaatttgat tgcctttgga gatgaacaca atgacaccga gatgctggcc tttgcacata
     1681 caggatatgc tatgaaaaat gctaatccaa ctctacttcc atatgccgac caacaaatcc
     1741 aatggacaaa cgaagaagat ggtgttgcta aaactctaga aaaattatta ttgtagtatt
     1801 ttcaaaaatg atctctattg aaacagaatt ctaatagaaa tcgtttttta ggttttctac
     1861 tatattatta acaaaaaaag ttaccactct cttcccctat tataaaaggt atttgtgaaa
     1921 aaaatatctt ttttttaatt tttattagaa atagtattga tttttttctg aaaacgtttt
     1981 aaaatatatc tgttctataa tttcgttgaa atgaaaaaga atggccaatt ttacgattgg
     2041 aaggagatta ttcttcttta tgtcaacgaa aaaatttttt aaggaggaag aacaagaatg
     2101 ggtatcggta ttattattgc cagccatggt aaatttgctg aaggtattca tcaatcaggt
     2161 tcaatgatct ttggtgaaca agaaaaagtt caagttgtaa ctttcatgcc aaatgaagga
     2221 ccagatgatt tgtatggaca cttcaacaat gctatcgcac aatttgatgc tgatgatgag
     2281 gtgcttgtgt tagctgacct ttggagtggg tcaccattta accaagcaag ccgtgttatg
     2341 ggagaaaatc ctgaacgtaa aatggctatc atcactggtc tcaatttgcc aatgcttatc
     2401 caagcctata ctgagcgtat gatggacgct aatgcaggtg ttgaacaagt tgccgcaaat
     2461 attattaaag agtctaagga aggtatcaaa gcccttccag aagaacttaa tcctgttgta
     2521 gaagctactc ctgtagcagg tgttcctgca gatgttcctg cggaagttaa acaaagtgga
     2581 tctatccctg aaggaactgt tattggagat ggcaagctta aaattaatct tgctcgtatt
     2641 gacacacgtc ttttacatgg tcaagttgca actgcatgga ctccagcatc aaaagcaaac
     2701 cgtattatcg ttgcttctga tgaagtttct aaagatgaac ttcgtaaaca attgattaag
     2761 caagctgctc ctggaggagt caaagcaaac gttgtcccaa ttagtaaatt aattgaagta
     2821 gctaaagacc cacgtttcgg aaacacacgt gccttgatct tatttgaaac tgtacaagat
     2881 gcactccgtg caattgaagg tggtgtggaa atccctgaat tgaacgttgg ttcaatggct
     2941 cactcaactg gtaaaacaat ggtaaacaac gttctttcaa tggataaaga cgatgttgct
     3001 gcctttgaaa aattacgtga cttaggtgta tcatttgatg tacgtaaagt accaaatgac
     3061 gctaaaaaga atttgtttga tttaatcaac aaagcaaacg taaaataact tattatctct
     3121 gaaaacgaaa ggatatcaaa atggcggata tttcaattat ctcaatgatt ttggtcgtta
     3181 tcgttgcctt ctttgctggt cttgaaggta tcctcgacca attccaaatc catcaacctc
     3241 ttgttgcttg tactttaatc ggtcttgtaa ctggtcacct cgaagcgggt attattcttg
     3301 gtggtagtct ccaaatgatt gctcttgctt gggcaaatat cggagctgcc gtagcaccag
     3361 acgctgcact tgcctctgtt gctgctgcta ttattttggt taaaagtggt gacttcactc
     3421 aaaaaggtat caactttgcc ttctctactg ctgtacctct agctattgct ggacttttcc
     3481 ttacaatgat tgttcgtaca atctcaactg cacttgttca cgctggtgac aaagctgctt
     3541 ctgaaggaaa cttcgcagca attgaacgtt ttcactttat cgccctcctt ctccaaggat
     3601 tacgtattgc attcccagct gcgcttcttt tagctattcc atcttcttca gtacaaagta
     3661 ttcttgaagc aatgcctgat tggttaaatg gtggtatgca agttggtggt gccatggttg
     3721 ttgccgttgg ttacgctatg gttatcaata tgatggctac tcgcgaagtt tggcctttct
     3781 ttgctcttgg atttgctcta gcagctttga accagttaac tcttattgca atgggtacta
     3841 ttggtgttgc tatcgccctc atctacatca gtttatcaaa aatgggtgga agcaaaggta
     3901 cttctaacgc aggttctaac gatcctatcg gcgatatctt agaggactac tagaaaggag
     3961 ctaaacaatg actgaacaaa ttaaattatc aaaatcagat cgtcaaaaag tttggtggcg
     4021 ttcacaattc cttcaaggtt cttggaacta tgagcgtatg caaaatatgg ggtgggccta
     4081 tgctcttatc ccagcattga aaaaacttta cactactaaa gaagatcgtg ctgctgcgct
     4141 tgagcgccat atggaattct ttaacacaca tccatacgtt gctgctccaa ttatcggtgt
     4201 aacccttgct cttgaggaag aaaaagcaag cggtacacct gtcgaagaca aagcaattca
     4261 aggggttaaa atcggtatga tgggacctct tgctggtatt ggtgatccag tattctggtt
     4321 tactgtacgt cctatcttag gtgctcttgg tgcttcactt gcctctgctg gtaatatcct
     4381 cggaccaatc atcttctttg ttggttggaa cctcattcgt atgtcattct tatggtacac
     4441 acaagagtta ggttacaaat caggtaaaga aattactaaa gatatgtctg gtggtatcct
     4501 ccaagatatt actaaaggtg cttcaatcct tggtatgttc atccttgctg tccttgttaa
     4561 gcgttgggta gcaattaact ttactgtaga ccttcctaaa aagacattgt cagaaggggc
     4621 atacatcaat ttccctaaag accatgttag tggacaacaa ttgcatgata tcctaggaca
     4681 agttcagagt ggcttaagcc ttgataaaat gcaaccacaa acacttcaag gacaacttga
     4741 ctcacttatt cctggtttag ctggattact tcttacattc ttctgtatgt ggttacttaa
     4801 gaagaaagta tcaccaatta caattatcat cggtttgttt atcgttggta ttctagctcg
     4861 ccttgcaggt gtgatgtaat aaaactaaga gatggttaca atagtattcc atctcttata
     4921 ttttattaca taaaatccac ttagagtccc actattatga tataatcaaa ctactataaa
     4981 ttaaaaaggg attagtcatg gcacaatcat taaacaaaac tgttgaattt caaacaacag
     5041 gagtttcgta tttaggtatg gggaataaag ttggaaaatt tttagttggt gaccaggcat
     5101 tagaattcta taacgataaa aatgtcaacg actatattca aattccatgg acatcaatca
     5161 atcaaattgg agctaatgtt agtcgaaaaa aaattagtcg tcactttgag gtctttacag
     5221 accaaggaaa atttttattc gcctctaaag actctggaac gatattaaag catgcgagac
     5281 gacatattgg agacgataaa gttgttaagc ttcctaccct catccagacc attttaaaaa
     5341 tattcaagtt taaaaaataa gatagactat agtctatctt attttttaac tctaatatta
     5401 atatgggaac ttgtcatcaa accatctcaa aaagttagaa aaatggattg ctttaaatat
     5461 tcgtaatcta atacgttcta caataaaagc caacaaataa attaaaatcc ctgctcctaa
     5521 aattaaaaat gggtaaagat aaatggactc ataaacaaac gtctctgcaa aatctcttac
     5581 aaaatatttt actattagtg ggtggagatg acagagataa actcccaagg ttgaaggggc
     5641 taatacaaca attattttct ttaagaatcc cgagggctta atgcttgctc tagcaaataa
     5701 tataaacaaa cttaccgccc ctaaagttaa agtaggcgaa acataccaat accaaatatc
     5761 accaacgcta aacttcatgg cataagtagc aaccaatgat aatagataaa ttataagtaa
     5821 atatgacgtt ttaaatatat ttaaatcaat acgtttcaaa taggcaccaa taatatataa
     5881 aatcagtagc caagtcattt caaaaccctt gctcaatgaa aattctggaa cacgattatt
     5941 taaaacagct ggtaaaattg aaaaaataat aaacattaac aaaaccaatt gttttaattg
     6001 tttatcagtc aaggcattta atccattatt aataacaggc ataaaaacca atagaccaaa
     6061 gtaagcagta atgtaccaat actgcccact gacaataggg aaaaaagcat ctctccaatt
     6121 aagcaaggtc acttcatgac ctgtgattgc aaaaagtgct gttattgtaa aggtgtagaa
     6181 aaaaacctgt gcccagatag acagtaattt tgagtaacga taacgcgaat taattcccac
     6241 ataaccactt attagtgcgt aacagttaac ggccccataa actaagactt ggataatcca
     6301 cgttactata aaataggagt ctgcgtgtcc ctcaaccgaa gatctcagac caccttttcc
     6361 aagaacatgc gtaataacaa tcatgaacat ggaaataatc cttaaaaggt ctaagccgta
     6421 atgacgacct gattgtttaa tacttgtata cttttccata atcatatttt agcatagcta
     6481 tttcactaaa acaaagagca ggcttataaa gttaaggttt acattttact tatttttgga
     6541 tataataacc ttaacggata agtagtataa tagttctttt cagagagtct gcggtcgttg
     6601 cgagcagata agattgtttt atgaaattct accgtgttcc atttatggga aataatttaa
     6661 tcaatcaatc aagtgcactt ctgtgaagct ggatggaacc gcgcctttgc gctccagcat
     6721 gacagggtgt tttttagtat aaaggagatt cctatatgtt agacttaaaa cgtattcgta
     6781 cagattttga tgttgtcgct aaaaaattgg ccacgcgtgg tgttgaccaa gaaacattaa
     6841 caacccttaa agaacttgat ataaaacgcc gtgaactact tatcaaagct gaagaggcga
     6901 aagcgcaacg taatgttgct tctgctgcca ttgctcaagc aaaacgaaat aaagagaatg
     6961 cagacgagca aatcgcagct atgcaaacac tttccgctga tatcaaagca attgatgcgg
     7021 aattggcaga tgtggatgct aatttacaat caatggtgac cgtacttcct aatacaccag
     7081 ctgatgatgt gccgcttgga gcagacgagg atgaaaatgt tgaagttcgt cgctggggaa
     7141 ctcctcgtga atttgatttt gagaccaaag ctcactggga tttaggtgaa agccttggaa
     7201 ttctcgattg ggaacgtggt gcaaaagtta ctggctctcg cttcctcttc tacaaaggtc
     7261 taggtgctcg tttagagcgt gctatctaca gcttcatgct ggatgaacac gctaaggagg
     7321 ggtacactga agtgatccct ccttatatgg ttaaccacga ttcaatgttc ggtacgggac
     7381 aatatccaaa gttcaaggaa gatactttcg aattagcaga tagcccattc gtacttatcc
     7441 caacagctga agttccttta acaaactatt atcgtgatga aattatcgat gggaaagaat
     7501 tacctatata ctttactgcc atgagtccat cattccgttc agaagcaggc tctgcaggac
     7561 gtgacacacg tggattaatt cgccttcatc aattccataa agtagaaatg gtgaaatttg
     7621 ccaagcctga ggaatcatac caagaattag aaaaaatgac agcaaatgct gaaaatattc
     7681 ttcaaaaact taatctccca taccgtgtca ttaccttgtg tacaggagat atgggattct
     7741 cagcagctaa aacgtatgac ttagaggtat ggattcccgc tcaaaatact tatcgagaaa
     7801 tttcttcatg ttctaatact gaagatttcc aagcacgtcg tgctcaaatt cgttaccgtg
     7861 acgaagttga tggcaaagtt aggttgcttc atactttaaa tggttcagga cttgcagttg
     7921 gacgtactgt agcagcaatc ctcgaaaatt atcaaaatga agatggttct gtgactatcc
     7981 cagaagttct tcgtccatat atgggtaata tcgatattat taaaccaaat taggcactct
     8041 aaaatgagtt ggtataccaa ctcatttttt ctatgaactt agctaatagt ttgacaaact
     8101 tgatagtaag gtaacacttt ctcaaagcct tctaacaatc gttgcagaat attttctaag
     8161 ctctggtttt cagtcaaaag aacgtcatat tttaccaata ctttccttac cttccctgac
     8221 ttaatttctt gcattaaacg ttgacgattt tcctcattgc cttcttctcg atgagtttca
     8281 ccttgccttt ggaccataaa atacaatgga gaggcaatcg gaatcgataa cactctagcc
     8341 tgcttctcga gtgtcttatc agatttttgc ctctctacaa agcttacctc aaggctaacc
     8401 ccaaaactat cttttacacc ataaaccctt agcgcaacag ccacatcatc taattggtct
     8461 gtatcctttc gataataaac ccaaaaatgt ggacgcccta cttgagcttg attagcccaa
     8521 ttactcactc tttgcatttt aaagccatcc atacgttgtt ctaaggctct tgataactct
     8581 gtaaagctat tcctagcaag ttggcccttt gccttaaaag tttgcatatc gtcctttaaa
     8641 gggcctgcct tctcaggcat aatatacttc attgattgga attctaaata atcatttaat
     8701 tcttgataca ttctttttct ccatgtaaac aatagttaag ttttcacgat tatatatatc
     8761 tatacatact ttcaagttct tttaaagtat ttctagcgaa gcctcgttgc tagaaacaag
     8821 gtctaatcgc aattataact aaccaagctt tactttcttc atcacttaat ggaactcttt
     8881 aaaaaccaat cttttcatag ccttctaaaa tctaataata gctatttttt ctagaaaatt
     8941 tctcttcaat aacagataga gtctcaagtt aaggagagcc taaataatgg tacttagaat
     9001 aaagtgttcg aaatgactct ttctgaaaat tttggattag actacaatct aaaaattggc
     9061 taatgctagt gtcattgata tatctccttt ttatcaatta taacaaaata tgaaactata
     9121 actatgataa taccttgaaa aactaaatat aggctgagta aataactcag cctttttcta
     9181 ttaatatttt ctaaaacgtt ggtaacgttg ttccaataaa tcctcaagag ataattgtga
     9241 gagcacttct aactctgaaa ttaaacttgt cttaatcata tcgacaatct cacttgaaaa
     9301 ataaccatgt tcagggatta ctttgtctac aacttccata tggtaaagtt ctccagcagt
     9361 catcttcatt aattgtgctg cttctgttgt tcttgtacca tctttccata aaatagaggc
     9421 aaagccctca ggacttaaaa tagaataaac agtatgttcc aacatccaaa ccttatcggc
     9481 aacagctaaa gctaatgctc ctccagaacc cccttcacca ataataatag ctataatagg
     9541 gacttttaaa tcactcattt ctaagaggtt tctagcaatt gcttcaccct gacctcgctc
     9601 ctcagcacct accccaggat atgctccagc agtattgatg aaggttataa caggtcttcc
     9661 aaacttctca gcttgtttca ttaaacgtaa agccttacga taaccttcag gatgtggttg
     9721 accaaaatga cggtctaaat tatcttgaag gttcttacct ttttgaatac cgacaatcgt
     9781 cacaggacgc cctgccaagt accccaaacc tcctataatt gatttgtcat ctgcgaactg
     9841 gcgatcacca tgaagttcca taaaattatc aaaaatcaat tcagcgtagt caagagcagt
     9901 tagcctccct tgatcacgcg catcttttaa aattcttgtc acatcattat tcatgaccaa
     9961 cctcatttcc atggaaagca attagttgag caatgcagtc gcgtaattct gttcgattta
    10021 taatagcatc gacaaaacca tgttctaaca aaaactctgc cttttgaaag ccttcaggta
    10081 aatcttctct cactgttgtt tcgataacac gacgaccagc aaaaccaacc aacgcttgtg
    10141 gctcagccag aataatatca ccttccatag caaaactcgc agtaacgccg ccggttgttg
    10201 ggtctgttaa aattgttaaa taaaacaatc cctggttaga atggcgttta actgctgccg
    10261 atacttttgc catttgcatc aaactcataa taccttcttg cattctggcg ccccctgacg
    10321 ctgtaaaaat aacaataggt aatttctttt cagttgctag ctcaaataat cttgtcaact
    10381 tttcgcctac gaccgttccc atagacgcca ttataaaatg agaatccata atagctaagg
    10441 ccgtagtttg tccttttatt ttagctaaac ctgtcaccac cgcttcatct aagttagtct
    10501 tttgacgtgt agcagccagt ttttcacggt agtttggaaa attcaaagga tccttagttt
    10561 caattcctgt aaataattcc tcaaaactat cttcgtcaac tgttagcaac agacgttctt
    10621 gcgctgaaat tctaaagttg taagaacaag ctggacaaat ctttgctaat cctaaatctt
    10681 tctgataaat catatgttta caagaaggac acttagcaaa gagttcatca ggaacctcag
    10741 gaagactacg tttatcagaa gaccctaatg ctttgtttgg actaatacgg atatatttat
    10801 cctttttact aaataaagcc atcttccctc ctattgattg ttaccataat accttggtaa
    10861 aaagtcttcc atcaggaatg aagtatcata atccccagcc aagacttttt tatcagaaat
    10921 gagatccatt tgaaattctg tatttgttac aataccatca atttcaagct catataaggc
    10981 acgttgcatt ttcataagtg catcaaaacg attttctccg tgaacaatta ctttcgcaat
    11041 catactatcg taataaggag gaatagtgta gcctgtgtaa accgctgaat cgacacgtaa
    11101 gcccataccg ccagcaggta gatgtaaccc attaatggta cctggacatg gcgcaaaatt
    11161 aaattgtgga ttctctgcgt taatacgaca ttcaatcgca tgaccagtta acttaatatc
    11221 attttgagaa actgataaag gaattccagc agcgatacga atctgctctt taacgatgtc
    11281 aacacccgtt acaaattctg taacagggtg ttctacttga acacgagtat tcatttccat
    11341 gaaatagaac tgtccgctat tctcatctaa caaaaactca attgttcctg cattttcata
    11401 agatacagcc tcagctgcac gaacagctgc ttcaccaatt tgttgacgca gagtgttacc
    11461 tattgccaca gacggacttt cttccaatac cttctgattg ttacgctgca gagaacaatc
    11521 acgttctcct aggtgaacta tcttaccaaa gctatctcca agtatttgta cttcaatatg
    11581 tctagctggg tatataactt tttcaatata catagcacca ttaccgaacg cagccaaagc
    11641 ctcctgtgaa gcagactcaa aagcaggctt caattcatct gctgatttga ccttcctaat
    11701 tccttttcca ccgccaccgg ctgaggcttt taacatcaat ggatagccga tttcctcagc
    11761 aattgataca gcttcttcta cacttgtaac ttgaccatct gatcctggga taaccggaac
    11821 atcagctttt atcatctctg tacgagcatt gattttatca cccattttat ccataacttc
    11881 tccagatgga ccaatgaatt ttaagttcat ctcttcacac atagtagcaa atttagaatt
    11941 ttcactcaaa aaaccaaaac ctggatgaac agcttccgct ccagtcacaa tagcagcaga
    12001 taatatagca ttaacgttta aataagattc tgctgatttg gctggtccaa cacaaatagc
    12061 ttcatctgct aaaattgtgt gcaatgattc cttgtcagcc tccgaataga tagcaactgt
    12121 cgaaattccc atttcacggg ctgcacgaat gatacgtact gcaatttctc cgcgattagc
    12181 aatcaatatt ttcttaaaca tttttgatgc ttctctccct ctaaattcaa tactatcttc
    12241 aattgcctat tgcaaatgtt aacgttccac tggcagctaa tttcccatca acttctgcta
    12301 ttgcttcaac aacggctata gttcctctac gtttgacaaa cttagcagtc ataactaatt
    12361 gatctcctgg aacaacttgc tttttaaatt tcactttatc catacctgca taaaaaacaa
    12421 gctttccttt gttttcttct ttagataatt ctaaaacacc tgcagtttga gccaaagctt
    12481 ccataattaa aacgcctggc attacaggat attctggaaa atgaccattg aaaaaaggct
    12541 cattgatact aacatttttg atagcgacta tctcatcttc tgatacctct aaaacacgat
    12601 ccactaataa cattggatag cggtgaggta aagcctcacg aatttctttt atatcaatca
    12661 tttgattcgt accaatcctt taccaaattc aataacttct tcattggcta caagaatttc
    12721 agtaactaca ccatcatggg gggcaggcac ttcattcatc accttcattg cctcaataat
    12781 catcaaagtt tgtcccttct tcacgctatc acctactgaa acaaagttag gtttatctgg
    12841 tccagaagct aaataagcaa ccccaactaa aggactttcg acaacgtcac cttctgcaac
    12901 agtcacagtc gactctggga tttctgaaac tacttcagta gcttgtttcg tttgctcacc
    12961 tgcttcatta gagacaggag ttgctatagt tggagtcgct tcaggttgat gactcatagg
    13021 agacatagtt gggacaagtg gagcttttcc ctcattttta ctaaaagaca gctcaccgtc
    13081 acttgttttg aatgaaaatt ctctcagact tgactcatca aattgagtca ttaaatcctt
    13141 aatctcttga atatccaatt taatcctccc agcgtttgaa ggccagaact gcattgtgtc
    13201 ctccaaaacc aaatgtattt gaaatggcat aacgaatatc agcgtcttgc ccttgaccaa
    13261 aaataacatt cgctgtaata tcttctgata attctgtcgt tccagcagtc attggaacat
    13321 aactatgacg tatcgcttca atagttgcaa ttgcctcaac agctcccgcg gcacccaaaa
    13381 gatgaccagt aaaagattta gtcgaagaaa ctggaacgtc tgtcccaagt gcagcaacaa
    13441 tagcttgact ctctcctttt tcgttagcag gtgttgaagt tccatgagca tttacatagt
    13501 taacttcttc aggtttaata ttagcttcaa ccagtgcaag ttgaatagct ttagtggctc
    13561 ctaaaccttc tggatgtggt gaagtcatat gatatgcgtc acaagtgtta ccataaccaa
    13621 caacttctgc aagaatagta gctcttcgtt tttcagcgtg ttctaaactt tctaagacca
    13681 acattccaga accttctccc atgataaatc cattgcgatc tttatcaaat ggaatagatg
    13741 ctcttgatgg atcttcagtt gttgataatg ctgtaagact ttggaatcca gcaatggcaa
    13801 attttgtaat agcagcttct gcaccaccga ctaccataat atcttgaata ccaaatttta
    13861 tattacggaa agcatctcca atagcatcat tagaagaagc acaagctgta ttaattgatt
    13921 tacatacacc atgagcgcct aacctcatcg caacatttcc agctgccata tttggtagag
    13981 cttttggaag cgtcattggt tttacgcgtt ttggcccttt ttcatgaaga cgaatcactt
    14041 gttcttcaat ctcttgaatc cctccaatac ctgatgcaac gatgacacca aatcgatctg
    14101 catcaatttc atctaaattt aaattagcat gttgaatagc ttcacttgaa gcatataagg
    14161 cataaaggga atacatatca aatctgttca gatctttttt gacaaaatat ttatcaaatg
    14221 gaaaatcatg aatttcagca gcatttttga ccatgaaatc acttgaatca aatttagtga
    14281 taggtccaat accaacattt ccttctttaa gactattcca aaattcttct ggtgtgtttc
    14341 caataggtga ggttacaccg taacctgtta ctactactcg ctgtaatgtc atattatttc
    14401 ctctttatca ttgcatcgtc ataccgccat caatggcaat aacttgtcct gtaaggtatt
    14461 cttgttctgc taagaaagac gctacctgcg ctacttcttt accttttcca atacgtttca
    14521 ttggaatttg agctaaaata gcttcttgca ttttttctgg gataacatct gtcatatcag
    14581 attcgataaa gcctggagca atggcattga cacgaatgcc tcgtgctgct acttctctgg
    14641 caactgattt tgtaaagcca attaaacctg ctttagaggc tgcgtaatta gcttgcccta
    14701 catttccagt taatccaacg acgctagaga tattaataat agcaccttga cgcgcttttg
    14761 tcatcggttt caagactgat tgtgtcatat taaatgcacc tgttaaatta attttaagaa
    14821 cgctctcaaa atcttcaaca gtcattttaa gcatcaattt atcattagta ataccagcgt
    14881 tgttaacgag cacatccacg ctacctagac ttgcaatagc ttctttaatc atgcgattag
    14941 catcttcaaa agaagaaaca tctcctgaaa tagcaataac ttttacacca taatcagcaa
    15001 attcagctat taagtcttct gatatttcag aacgtccatt taaaacaata ttagcaccta
    15061 attgtgcaaa ttgatgggca attgcaaggc cgattcctct acttgaaccc gtaataaaaa
    15121 tgttcttatc ttttaattgc atattttccc ctagtttaat tccttaagat tattaaatcc
    15181 aattttatct tcaacagaat gtactgataa tgagctatct atttttttca agaaacctga
    15241 gagtaccttc cctggaccaa tttcaacaac ctgtgtcatt cccatttttt tcatggtttc
    15301 aatagactca tcaaagcgta caggttccat aacttggcgt gccaataaag atttgatatc
    15361 gtcttttttc attatctgcg cctctgtatt tccaattaca gggatttcag aaactgaaaa
    15421 atgaacttta tctaaaacat cgctcaattt ctgacttgca ggtttcaata aagcagtgtg
    15481 aaaaggacct gaaacattca gaggaattaa acgttttact ccttgttgtt ttaattcttc
    15541 tacagctgca tttacagcga ttgactctcc ccctataaca atttgctttg gagtattata
    15601 atttgcagga ctaacaatac caaactgaga agccttctta caagcatctt caattatttg
    15661 acgatcagca tttaaaactg ctaccatttt gccactacca gcaggtgctg ctgcttccat
    15721 tagttggcct cgtctagcaa caagtactac agcatcttca aatcttatag cacctgaagc
    15781 aactaaagca ctgtattctc ccaaagaaag tcctgcaacc atatctggtc taagttcaat
    15841 ttctttaaga attaaacgat aaatagcagt cgaagtcgtt agaatagctg gttgcgtata
    15901 tttcgtctga tttaatttgt ctaagtcttt atcaatcaat tctctcaaat catatcctaa
    15961 aacatgactt gccttatcaa aagtttcttt aacaatcggg aaagtttcat ataaatctct
    16021 agccattcct aacttttgag caccttgacc agcaaataga aatgcaactt tattcatagt
    16081 gtctccttat ttgtatttat aaatctgacc agcggcttgc ctcagaaaga ataacttgac
    16141 gcgctcctga ataaatatct tcaagaatct cttgacaagt ctcttctgat ttaattaatc
    16201 ctgcaatttg tcctgccata actgaaccat taataacatc cccatcaaca actgcattgc
    16261 gaagagctcc cgctcctaat tcttcaatct cattgattga aatacggcca gctaaataat
    16321 ctttttctgc ttggctatag gttgttacta gtttattttt taaggcacga actggatgac
    16381 ctactacctg agcagaaaca gcagtgtcga tatcttttgc ttttaagatt tttgctttgt
    16441 aattagggtg agcattagat tctttagcaa caacaaaacg agttcctacc tgaacagcat
    16501 cagctcctaa catgaaaccc gcagcagccc cacgcccatc tgctatacca cctgcggcaa
    16561 tgactggaat tgtaacagca tcaacaacct gtcgtactaa tgtcatcgtg gtcagttttc
    16621 cgatgtgacc acccgcttcc attccttctg taatgatagc atcagcccct aatttctcca
    16681 tacgttttgc taatgcaaca ctaggaacaa cagggattac tgttatacct gcttcatgaa
    16741 agcgctccat gtacttacca ggattacctg cacccgtcgt tacaactttt acaccttctt
    16801 caataaccaa atcaacaata tcatctacaa aaggtgataa aagcataata ttcacaccaa
    16861 agggtttatc agtcatagat tttattttat caatatttgc tttgacaacc tctttagggg
    16921 catttcctcc accaataata cctagaccac ctgccttaga aactgctcct gcaagatctc
    16981 catcagcgac ccacgccata cccccttgaa aaattggata ttttatattt agtaattctg
    17041 ttatacgtgt tttcattttt ctttcctaaa attaataatt tgacaatcaa actatattga
    17101 taagaagaag agtgagtacc gatcaacgat tgattgaata ctcactctaa taagtgattt
    17161 ggtaagaata tcttttatta tcacttgctt ctttttttct tacgacttaa ttattttact
    17221 ttttcttcaa cataagcaac taaatcacca acagtattta gtccttcttc tgtctcaatt
    17281 tggatatcaa atgcatcttc aatttctgaa atcacttgga aaacatcaag cgaatcagca
    17341 tcaagatcgt caaaagtagt gttcaacgtt acttcttctg catctttacc aagttcttca
    17401 acaataattt cttgtacttt ttcaaatact gccattttta gctccttaaa ttttaaataa
    17461 tcttttataa atgtgctatg cactatatat aactaaattt taacaattag acttccccat
    17521 gtcaaacctc caccgaaacc cgataagagg attgtttggt taccatctag tttcaacaaa
    17581 ccgttctcat aagattctga aagtaaaata gggatactag ctgcactagt attaccataa
    17641 tccatcatat tctctggaaa tttatcacgt gctatatcaa tttttcgaga catcttatct
    17701 aaaatccttc gattcgcttg gtgtaagaaa agataatcaa tatcttcttt ttccaaataa
    17761 gatgtttcta taagatgatt gatacttttt gaaacttctt tgatagcaaa atcaaagatt
    17821 gctctaccat ccattttcaa aaatttatca tcaagtactt catctgagaa aggtgaattt
    17881 aacccgactt gactggattg aagtccctga cgactaccat ctgtattgag agactctgca
    17941 agaaaatgtt tttccttaga agcttctagc aagacacctc cagctccatc tccaaataaa
    18001 actgctgtcc ctctatcagt ccagtctaat actttagaaa cagtttctgc tcctattact
    18061 aaaccttttt gatagctacc tgatgatatc aatttttcgg cggtagacaa ggcaaaaaca
    18121 aaaccactac atgctgcact caagtcaaag gcaaaagcat tactcgctcc aatatgagct
    18181 tgcactcttg cagcagtaga aggcatcata gagtctggtg tcatcgttgc aacaataata
    18241 aagtcaattt gactagcgga ataaccggat ttttcaataa gttgttctgc gaccttattt
    18301 gccaaatcag ctgttgtttc atttttacta atatgacgtt gttttatacc agtacgtgac
    18361 gaaatccagt catcactggt atccatgatt aatgataaat cctcattttt aataatttga
    18421 gaaggtgcat aatgtgcaag ttgactaatt ttagcaaaaa ccactagact aagtcctcca
    18481 aaaatttatg aagattccct aaagcgctcg tcaacatttt tatgtcttct tcacctaggt
    18541 cttcaataat atgagatacc atagatttat gaaatttact atgcaatcga tctaataacc
    18601 tacctctctt agtaagactc aagtgaacaa ctcgacgatc aatacttgac cgtttacgct
    18661 caatgtagcc ttttttttct aacttattaa ggctggttgt tacagttccc aaagttaaca
    18721 ttaattctcg agcgacatcg ctaggagtta cctcagaatg tttcccaata atttcaatag
    18781 tatgcatttc ttttaaagat acatcgctaa attggctagt ctttaaactc atttcctcaa
    18841 taatcatgat tctattaaaa atgtcaacta aataactatt aatctgatcg tattccaaga
    18901 tatcaccacc tcacattaaa ctttgataat caaattatat caaagttcaa acttatttgc
    18961 aagactttaa tcttaaataa tttgattttc aaactatttg cctaaaaaat taggacgcct
    19021 tctatcagcg tgtgcccgaa caccttcttt aaaatcttgc ttaaaggcta atgattcttg
    19081 cagttgtaat tcaagctttt tatattgttc ccaatcttta aaagcagctt cccaagctaa
    19141 tgattttatg gcttgatatg agtttataga atgtctactt aaacgcttta atagttgatc
    19201 cttaatttta cctattttat ctgattcaca aagtttataa acaattccca aagcttcagc
    19261 tttttcagca gataagcttt ctccagttaa agctagttgc actgcacgag ttataccgat
    19321 tgatttacta agtaacaata aacctcctgc atctggagct aaaccgacac ccacaaaagc
    19381 ctggataaat ttactcttct tgcttgcgat aacaaaatca gctgctaatg caatattagc
    19441 tgcggcacca gctacagccc catctacaca cataacaaca ggtttctcta gctgtagtaa
    19501 atcatacgat atctgattaa ctaactcagc aattttagtt aatgagctaa tatcatcgct
    19561 ctcaacagca gctttcatca cttttaaatc tcctccgatt gaaaagattg gtccttgtgc
    19621 ttcaatcact aaaaacatga catctttatt ttcagacaca agccttattg catcaataat
    19681 ttcttgacac attgggatgt taaaaccatt tgaaatatct ggtcgattaa gcataatagt
    19741 ggccacgttg ccatcaatac catatattat attttcgaac ttcaaaatgt ctcctctctt
    19801 taagagtgaa agaaaaataa aaaataattt gattatcaaa ttatcatgct ttgtatttta
    19861 tcataaacct agctttcatg gcaagataaa aaataaaaaa acggaattaa acttcccgtt
    19921 tttaatagtt atctaatttc acaaaaaatt gactcagatc gctcactaat acattagcct
    19981 gcgactgatt cattccaaag tatttatctt caatagccca cacatcaata ccggctgcaa
    20041 taccggctgc aataccagct gtaatcccct tctcactgtc ttctacaata agaatttgct
    20101 gtttaggaat atctaaaagt tctgctgctc tattataaat agctggatta ggtttacttt
    20161 ctgaaaattc ctcaccagac agaattacct taaaatatgt atccagatta aaactttcta
    20221 atgctaacat gatatcatgt ctagtagaac ttgaagctag acccaaaaga tagcctttgt
    20281 gatgaagctt ttcaatgact cttttacaat cctgaaaaat taaatctttg tatggcaagg
    20341 gattatcctc tttatagcga ctataatctt gttggagctt atcaatatcc caggtatcat
    20401 attgatcacc taaaacagat ttccaaacct gcttcatatt tcctccaata aaaaaattca
    20461 ttggtaaatg gtcaattgta attccatgtt gttttaaaaa acgctctcgc cttttataat
    20521 aaaataattc agtgtcaaat agaacaccat
//
LOCUS       DATRNK010000034        20502 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.034, whole genome shotgun sequence.
ACCESSION   DATRNK010000034 DATRNK010000000
VERSION     DATRNK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20502
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.034"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            23..1213
                     /locus_tag="VBC52_001225"
     CDS             23..1213
                     /locus_tag="VBC52_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO6882815.1"
                     /translation="MNRPSEKEFKNSLFFKWILNNQAVIALMITFLVFLTIFIFTKIS
                     FMFKPVFDFLAVLILPLVISGLLYYLLKPMVTFLEKRGIKRVTAILSVFTIIILLLIW
                     AMSSFIPMMSNQLRHFMEDLPSYVNKVQMETSSFIDHNPWLKSYKGEISSMLSNISSQ
                     AVSYAEKFSKNVLDWAGNLASTVARVTVATIMAPFILFYLLRDSRKMKNGFLMVLPTK
                     LRQPTDRILREMNSQMSGYVQGQIIVAITVGVIFSIMYSIIGLRYGVTLGIIAGVLNM
                     VPYLGSFVAQIPVFILALVAGPVMVVKVAIVFVIEQTLEGRFVSPLVLGNKLSIHPIT
                     IMFILLTSGAMFGVWGVFLSIPIYASIKVVVKELFDWYKAVSGLYTIDVVTEERSEEV
                     KNVE"
     gene            1203..2429
                     /locus_tag="VBC52_001226"
     CDS             1203..2429
                     /locus_tag="VBC52_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882816.1"
                     /translation="MLNSEKMIVSIQNQDLEHANKYFEKALKNDPEEILLELGAYLES
                     IGFLPQAKRLYDQIRPNYPEVAINLAQIVAEDGDIEQAFLYLDYISEDSQEYVSALLV
                     MADLYDMEGLTDVAREKLLLASKLSDDPLVTFGLAEMNLSLEHYQEAIEGYASLDNRE
                     ILETTGVSTYQRIGKSYAIIGKFDAAIEFLEKAVDIEYDDLTVFELATILYDQEEYQK
                     ANLYFKQLDTINPDFDGYEYIYGLSLREEHKSEEALRLVQQGLRKNSFDGQLLLLASQ
                     LSYELHDVPSSESYLKQAEKVSENQDEIVMRLSNLYLEEERFEEVLELDNDDLENILA
                     KWNIAKAHKALEMDDSVDYYQSLYNDLKDNPEFLQDYAYILREFGYLDKAQEVGKAYL
                     KLVPDDIEMSEWVNNI"
     gene            2539..4221
                     /gene="alsS"
                     /locus_tag="VBC52_001227"
     CDS             2539..4221
                     /gene="alsS"
                     /locus_tag="VBC52_001227"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEO6882817.1"
                     /translation="MTESHNQYGADLIVDSLINHDVKYVFGIPGAKIDRVFDTLEDKG
                     PELIVARHEQNATFMAQAVGRITGEPGVVIATSGPGISNLATGLVTATDEGDAVLAIG
                     GQVKRGDLLKRAHQSMNNVAMLEPITKYSAEVHDPNTLSETVANAYRLAKSGKPGASF
                     ISIPQDVTDSPVSVKAIKPLSAPKLGSASVLDINYLAQAINNAVLPVLLLGNGASSEG
                     VTAAVRRLLDAVKLPVVETFQGAGIVSRELEDETFFGRVGLFRNQPGDMLLKRADLVI
                     AIGYDPIEYEARNWNAEISARIIVIDVEQAEIDTYFQPERELIGDMAHTLDLLLPAIK
                     GYELPEGSKEYLKGLRNNIENVSDVKFDRDSAHGLVHPLDLIDVLQENTTDDMTVTVD
                     VGSHYIWMARYFKSYEARHLLFSNGMQTLGVALPWAISAALLRPNTKVISVSGDGGFL
                     FSAQELETAVRLHLPIVHIIWNDGKYNMVEFQEEMKYGRSSGVDFGPVDFVKYAESFG
                     AKGYRVDSKDSFEETLKQALIDAENGPVLIDVPIDYKDNVTLGETILPDEFY"
     gene            4235..4954
                     /gene="budA"
                     /locus_tag="VBC52_001228"
     CDS             4235..4954
                     /gene="budA"
                     /locus_tag="VBC52_001228"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEO6882818.1"
                     /translation="MSETVKLFQYSTLSSLMAGLYKGSLTIGELLTHGDLGIGTVHMI
                     DGELIVLDGKAYQAIGTDGKAEIIQLSDDVTVPYAAVLPHHIQKQFDINAETDNKDLE
                     EMILKNFEGQNLFKSLKIKGTFSRMHVRMIPKSPQHKRFADIASNQPEFTKENVSGTL
                     VGIWTPELFHGVGVKGFHVHFISDDLTFGGHVMDYSLTQGKVEIGKVDQLDQCFPTQD
                     QEFLEANFDLQKLREDIDLSE"
     gene            complement(5008..6663)
                     /locus_tag="VBC52_001229"
     CDS             complement(5008..6663)
                     /locus_tag="VBC52_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEO6882819.1"
                     /translation="MSFDGFFLHHLTNELQEQIEKGRIQKINQPFDHELVLTIRNNRR
                     NYKLLLSAHPVFGRIQTTEANFQNPQNPNTFTMIMRKYLQGAVIETIQQIENDRILEI
                     VVSNKNEIGDHIKATLVVEIMGKHSNIILIDKNEHKIIESIKHVGFSQNSYRTILPGS
                     TYIAPPKTKAINPFDISDQTLFELLQTNDLSPKNLQQLFQGLGRDTALELSNCLKDNK
                     LNDFRQFFSREYHPSLTEKSFSAVQFSSSHETFQSLGQLLDYYYQEKAEKDRIAQQAS
                     DLIHRVQSELEKNIKKLAKQQDELLATENAEEFRQKGELLTTYLSIVPNNQDVVVLDN
                     YYTNQTIEISLDRALTPNQNAQRYFKKYQKLKEAVKHLKGIISDTENTITYLESVETS
                     LNHASMEDINDIREELVETGFIKRRAHDKQHKRKKPEQYLASDGKTIIMVGRNNLQND
                     ELTFKMARKGELWFHAKDIPGSHVLIRDNLNPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEVKKLNKPSGTKPGFVTYTGQKTLRVTPTQEKIDSLKLKK"
     gene            7022..8026
                     /locus_tag="VBC52_001230"
     CDS             7022..8026
                     /locus_tag="VBC52_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6882820.1"
                     /translation="MKNKGLIATLILLTILVVGELFYNKSEKRLNLSEKQVVKIGILQ
                     YVTHDALDAIEKGVEDGLAQEGYKGKKVKLTVLNAEADQSKIQAMSKQLVNHHNDILI
                     GIATPSAQGLAASTKDTPIIMGAVSDPLGAKLVTNMKKPTTNVTGLSNVVPTKQTVQL
                     IKDITPNVKRIGILYASSEDNSVSQVTEFTKYAQKAGLEVLKYSVPSTNEIKTSMSVM
                     TKKVDAVFVPQDNTIASAFRTVIVAANQANIPVYSSVDTMVEQGSIASVAQSQYGLGL
                     ETAKQAIKVLRGKPVKDVPVKVIDTGKPSLNLKAAKHLGIKIPKKIMKQAEITVKVDD
                     "
     gene            8039..8902
                     /locus_tag="VBC52_001231"
     CDS             8039..8902
                     /locus_tag="VBC52_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6882821.1"
                     /translation="MIISSVSQGLLWGILGLGIYLTFRILKFPDMTTEGSFPLGGAVC
                     VTLMNQGVNPILATILGMLSGMLAGFVTGLLYTKGKIPTILAGILVMTSCHSIMLMVM
                     KRANLGLNEIQTLKDFLPFSNDLNLLVLGLIAILLVISALIYFLYTRLGQAYIATGDN
                     PDMAKSFGIDTDKMEMLGLIVSNGLIALSGALVSQQDGYADVSKGIGVIVIGLASIII
                     GEVLYSTGLTLFERLIAIVVGSILYQFLITAVIALGFNTNYLKLFSAIVLGICLMIPV
                     LKTKILKGVRL"
     gene            8902..9663
                     /locus_tag="VBC52_001232"
     CDS             8902..9663
                     /locus_tag="VBC52_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6882822.1"
                     /translation="MVMKIIELKEATVQVSNGLAEMKTILDHVNLSIYEHDFITILGG
                     NGAGKSTLFNVIAGTLMLSSGNIYIMGQDVTNLPAEKRAKYLSRVFQDPKMGTAPRMT
                     VAENLLVAKFRGEKRPLVPRKIINYTEEFQKLIARTGNGLDRHLETPTGLLSGGQRQA
                     LSLLMATLKKPNLLLLDEHTAALDPRTSVSLMGLTDEFIKQDSLTALMITHHMEDALK
                     YGNRVLVMKDGKIVRDLNQAQKNKMAIADYYQLFD"
     gene            9959..11620
                     /locus_tag="VBC52_001233"
     CDS             9959..11620
                     /locus_tag="VBC52_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO6882823.1"
                     /translation="MSDIKIMALGGVRENGKNLYAVEVNDSIFVLDAGLKYPENEQLG
                     VDVVIPNLDYLIENKKRVQGIFLTHGHADAIGALPYIIAEVKAPVFGSPLTIELAKLF
                     VKNSTAVKKFNNFHVIDSETEIEFQDAVISFFKTTHSIPESMGIVIGTKEGNIVYTGD
                     FKFDQAARKYYQTDLARLAEIGRDGVLALLSDSANATSNEQVASEYEVGDEIKSVIED
                     AEGRVIVAAVASNLIRIQQVFDAAAENGRRVVLTGFDIENIVRTAIRMKRIHIADENM
                     IIKPKDMTRYEDNELLILETGRMGEPINGLQKMAIGRHRYVQIKDGDLVFIVTTPSIA
                     KEAVVARVENLIYKAGGSVKLITQNLRVSGHANGRELQLLMNLLKPKYLFPIQGEYRD
                     LSAHAGLAQEVGMSADDIYIVKRGDIMVLEKDGFFHSGSVPAGDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVVITVSKKEKKIISKARVNTRGFVYVKKSRDILRESAELVNTT
                     VEDYLSKDTFDWGELKGKVRDEVSKFLFDQTKRRPAILPVVMEVR"
     gene            11677..12464
                     /locus_tag="VBC52_001234"
                     /pseudo
     CDS             11677..12464
                     /locus_tag="VBC52_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863793.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            12554..13264
                     /locus_tag="VBC52_001235"
     CDS             12554..13264
                     /locus_tag="VBC52_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEO6882824.1"
                     /translation="MRTLFRMIFAIPKFIFRLVWNIIWGIFKTVLVIAIILFGLYYYA
                     NHSQSEFANQLSDIIQTGKTFLNFADTNQFKNSFANLATDNVHHSENTRWDKNQATIY
                     IATRDSELVSAYKQAISNWNATGAFVLQTTDNPNADIIAKDYSDAKTQAAGVAETEKN
                     ALTNRISKVTVKLNTFYLKNKQFGYDHTRIVNTAEHELGHALGLGHNDQQHSVMQSKG
                     SHYGIQEVDIQTLKNLYA"
     gene            13502..14173
                     /gene="rpiA"
                     /locus_tag="VBC52_001236"
     CDS             13502..14173
                     /gene="rpiA"
                     /locus_tag="VBC52_001236"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEO6882825.1"
                     /translation="MDELKKLAGVTAAKYVKNGMIVGLGTGSTAYFFVEEIGRRVKEE
                     GLQVVGVTTSNRTTEQARGLGIPLKSADDIDVIDVTVDGADEVDPDFNGIKGGGGALL
                     MEKIVATPTKEYIWVVDESKLVETLGAFKLPVEVVRYGSERLFRVFKSKGYCPSFRET
                     EGDRFITDMGNYIIDLDLKKIEDPKQLANELDHTVGVVEHGLFNGMVNKVIVAGKNGL
                     DILEK"
     gene            14230..15441
                     /locus_tag="VBC52_001237"
     CDS             14230..15441
                     /locus_tag="VBC52_001237"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO6882826.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFEDTVLNKLDQAGIDTYAVGKINDIFNGSGINHDMGHNKSNSHGIDTL
                     IKTMGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDERLPEIISAMRDKDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFADISATVADNFGVDTA
                     MIGESFLQDLV"
     gene            15492..15899
                     /gene="arsC"
                     /locus_tag="VBC52_001238"
     CDS             15492..15899
                     /gene="arsC"
                     /locus_tag="VBC52_001238"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416958.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEO6882827.1"
                     /translation="MEKIRIYHNPNCGTSRNVLAIIRHCGIEPEIIYYLKTPPSRMEL
                     VELLLEMKLSARELLRTDVPAYEKFNLESSSVTDEEMIDAMIQDPILINRPIVVTSKG
                     AKLCRPCEAILTILPVKMEKDFVKEDGQIIQSL"
     gene            15938..16747
                     /locus_tag="VBC52_001239"
     CDS             15938..16747
                     /locus_tag="VBC52_001239"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO6882828.1"
                     /translation="MTLLEKINETRDFLQAKGVTAPEFGLILGSGLGELAEEIENPIV
                     VDYADIPNWGQSTVVGHAGKLVYGELSGRKVLALQGRFHFYEGNTMEVVTFPVRIMRA
                     LACHSVLVTNAAGGIGYGPGTLMLIKDHINMIGTNPLIGENLEEFGPRFPDMSDAYTA
                     TYRQKAHQIAEKQNIKLEEGVYLGVSGPTYETPAEIRAFQTMGAQAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQRIKEDFKGLVKSLVAEL"
     gene            16749..18005
                     /locus_tag="VBC52_001240"
     CDS             16749..18005
                     /locus_tag="VBC52_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEO6882829.1"
                     /translation="MVSRLYYAVKFMIAVLFMTVMAGVGAILMHYVLMFTEWLAFGDS
                     RENTLSLLNSVTPIKRVLSLTLVSFLASLSWYYLQIKPKQITSIKQQVVFKDSSVKKS
                     PYWLHIGHAFLQLIYVGAGGPIGKEGAPREFGAINAGKISDLLALKVLDKRLLIISGA
                     AAGLSAVYQVPLASVFFAFETLALGISLKNIVTLLASTFGAASIAQLVISTAPLYHIS
                     KMSLNSQSLAFMFLIVLCVTPIAISFRYLNQKVTERRIKNIKILLSLPVVSLIVSVLS
                     IVYPQILGNGNALVQEVFKGTSVSLIAILVVLKMIATLSTLYAGAYGGILTPSFSIGA
                     CLGFLLASISIPLLPHISIVTSMLVGAAIFLAITMRAPLTAVGLVISFTGQSVITIVP
                     LTIAVLFATAYDYFIRKMRSLYVNPY"
     gene            17989..18699
                     /gene="deoD"
                     /locus_tag="VBC52_001241"
     CDS             17989..18699
                     /gene="deoD"
                     /locus_tag="VBC52_001241"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO6882830.1"
                     /translation="MSIHIEAKQGEIADKILLPGDPLRAKFIAENFLEDAVCFNTVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAINPDIHVRE
                     LVLAQAAATNSNIIRNDWPEFDFPQIADFKLLDKAYHIAKEMDITTHVGSVLSSDVFY
                     SNQPDRNMALGKLGVHAIEMEAAALYYLAAQHNVNALAMMTISDNLNNPEEDTSAEER
                     QTTFTDMMKVGLETLISE"
     gene            18708..19475
                     /locus_tag="VBC52_001242"
     CDS             18708..19475
                     /locus_tag="VBC52_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEO6882831.1"
                     /translation="MTDFSTALKVLVDQYSYHNAFLLLQKHGPLNSDLLFLLEMMKER
                     RELNIDFLFAHQEQVVILQEKYNIKLLHNPYDLELLANYIMDLEAKVKNGLIIDFVRS
                     VSPILYRLFMILLAQEVPHLHDYIHNARDDHYDTWKFKELKESNHPVLLAFSERWHDS
                     RLTSKSLAECLQLTDLDEEVKSTIIQLRQFEKSVRNPLAHLIKPFDEQELYRTTQFSS
                     QAFLDQIIFLAKVIGVEYDTVNFHYDTVNKLIIKILE"
     gene            complement(19500..20423)
                     /locus_tag="VBC52_001243"
     CDS             complement(19500..20423)
                     /locus_tag="VBC52_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6882832.1"
                     /translation="MRIQQLQYVIKIVETGSMNEAAKQLYITQPSLSNAVRNLETEMG
                     IQIFIRNPKGITLTKDGMEFLSYARQILEQTALLEERYKGDNTSRELFSVSSQHYAFV
                     VNAFVALFNGTDMTQYELFLRETRTWEIIDDVKNFRSEIGVLFLNSYNRDVLTKLFDD
                     NSLIATTLFTTTPHIFVSKSNPLANRKKLSMKDLEDYPYLSYDQGLHNSFYFSEEMMS
                     QITHPKSIVVSDRATLFNLMIGLDGYTVATGILNSKLNGDEIVAIPLDVDDIIDIVYI
                     RHDKANLSKMGQKFIDYLLEEVSFNHKNKQS"
BASE COUNT         6528 a         3240 c         4138 g         6596 t
ORIGIN      
        1 ttcatcttga aaggatatgt gaatgaatcg accatcagaa aaagaattta aaaatagttt
       61 gttttttaaa tggatcttaa ataatcaagc agttattgct ctcatgatta cctttttggt
      121 atttttaacg atttttattt ttaccaaaat ctcttttatg tttaaacctg tgtttgattt
      181 tcttgctgtg ctgatattgc cgcttgtaat ttctggcttg ctttattacc tattaaaacc
      241 tatggttaca tttttagaga agcggggaat taagcgtgta acagcgatat tatcagtttt
      301 tactattata atccttctgt taatttgggc aatgtctagt tttattccca tgatgagtaa
      361 tcaattacgc cattttatgg aagatctccc ttcatatgtg aataaagtgc aaatggaaac
      421 aagttcgttt atagatcaca acccttggtt aaaatcttat aaaggggaaa tatcgagcat
      481 gttatctaat atcagtagcc aagcggtctc ttatgctgaa aaattttcaa agaatgtttt
      541 agattgggca ggaaatttag ctagtacagt tgcacgtgtg acagtagcaa caattatggc
      601 tccctttatt ttgttttatc ttttaagaga tagccgcaaa atgaagaatg gtttcttaat
      661 ggttttacca accaaactac gccaaccaac tgatcgtatt ttgcgagaaa tgaatagtca
      721 aatgtcaggg tatgtgcaag gacaaatcat tgttgctatt actgttggtg ttattttttc
      781 aataatgtat agtattatag gtcttagata tggcgtgaca ttagggatta ttgccggtgt
      841 gttaaatatg gttccctatt tgggaagttt tgtcgcccaa attccagtgt ttatcttagc
      901 gcttgtcgca ggacctgtta tggttgttaa agttgcgatt gtttttgtta ttgagcaaac
      961 tctagaggga cgctttgtct cacccttggt tttaggtaat aaacttagca ttcatccaat
     1021 tacaattatg tttattttat taacctctgg agcgatgttt ggtgtttggg gagtattcct
     1081 cagtattccg atttatgcat ctatcaaagt cgttgttaaa gaattgtttg attggtacaa
     1141 agctgtcagt gggctatata caatagatgt tgttactgaa gaaagaagtg aagaagttaa
     1201 aaatgttgaa tagtgaaaaa atgattgttt ctatccaaaa tcaggattta gaacatgcta
     1261 acaagtattt cgaaaaagcg ttgaaaaatg atcctgagga aatcttattg gaactaggag
     1321 cttatttgga gagtattggt tttttacccc aagcaaaacg tctctatgat caaatccgac
     1381 caaattatcc agaagttgct atcaatttag ctcaaattgt tgccgaagat ggagatattg
     1441 aacaagcatt cttatactta gattatatct cagaagatag tcaagagtat gtttcagccc
     1501 tcctcgttat ggctgactta tatgatatgg aaggcttgac tgatgttgcg cgtgaaaagc
     1561 ttttgctagc cagcaaactt agcgatgacc cattggttac atttggttta gctgaaatga
     1621 accttagttt ggaacattat caagaagcga tagaaggata tgcatcactt gacaatcgag
     1681 aaatcttaga aacgactgga gtttcaactt atcagcgtat tggtaaatcc tacgctatta
     1741 tagggaaatt tgatgcagct atcgaatttt tagaaaaagc cgttgacatt gagtatgatg
     1801 atttaacagt ttttgagtta gcaacaatcc tttatgatca agaagaatat caaaaagcta
     1861 acctttattt taaacagttg gatactatta atccagactt tgatggctat gaatatatct
     1921 atggtttatc tcttcgtgag gagcataaat ctgaagaagc tttacgtcta gttcagcaag
     1981 gtcttcgtaa aaatagtttt gatggtcaac ttctcttact agcttcacaa ttatcgtatg
     2041 aattacatga tgttccttcg tcggagtctt atttgaaaca agctgaaaaa gtttctgaaa
     2101 accaagatga aattgttatg agattgtcaa atttgtatct tgaagaagag cggtttgagg
     2161 aagtcttgga attagacaat gatgatttag aaaatatcct tgcaaaatgg aatatcgcta
     2221 aagcacacaa ggctttagaa atggatgata gtgtagacta ttatcaatct ttgtataatg
     2281 atttaaaaga taatccagaa tttttacaag attatgctta cattttgaga gaatttggtt
     2341 accttgataa ggcacaagaa gtaggaaagg cttatttaaa attagttcca gacgatatag
     2401 agatgtcaga atgggtgaat aacatttaaa taggaaaaca cttacagaaa aaaataaaaa
     2461 attcacaagt aaattttcac aatctgatgt aaaaactgtg atataattga atcattcttg
     2521 tgaaaagagg gagataaaat gacagaatca cataatcaat atggggctga cttaattgtc
     2581 gatagcctca ttaatcatga tgtcaaatat gtttttggta tcccaggagc taagattgac
     2641 cgcgtatttg acactctaga agataaaggt ccagagttaa ttgttgctcg tcatgagcag
     2701 aacgcgactt ttatggctca ggctgtcggg cgtattacgg gagaaccagg agttgttatt
     2761 gcaactagtg gacctgggat ctcgaacttg gcaacagggt tagttactgc gacagatgaa
     2821 ggtgatgctg ttcttgcaat tggtggacag gttaagcgag gagatttatt gaaacgtgct
     2881 catcaatcaa tgaataacgt tgctatgctg gaacctatta caaaatattc tgctgaggtt
     2941 catgacccta atactttgtc agaaactgtt gcaaacgcct atcgtcttgc taaatctggt
     3001 aaaccaggag caagttttat ctctattcct caagacgtta cagacagtcc agtctcagta
     3061 aaagcaatca aacctcttag tgcacctaaa ttgggttcgg catctgtttt agatatcaat
     3121 tatttagcgc aggctattaa caatgctgtt ttaccggtac tgttattagg aaatggcgca
     3181 tcttcagagg gagtaactgc tgccgtacgt cgacttcttg atgctgttaa actaccagta
     3241 gtagaaactt tccaaggtgc gggtattgtg tcacgcgagc tcgaggatga aacattcttt
     3301 ggtcgtgtag gtctcttccg taatcaacct ggagatatgc ttttgaagcg tgcagaccta
     3361 gttattgcta tcgggtatga ccctattgaa tatgaagcgc gtaactggaa tgctgaaatt
     3421 tctgctcgta ttattgttat tgacgttgaa caagcagaaa ttgatacgta tttccaaccc
     3481 gagcgagaac tgattggaga catggcacat acacttgatt tacttttacc agctattaaa
     3541 ggatatgaat tgcctgaagg ttcaaaagag tatttgaaag ggcttcgtaa taacattgaa
     3601 aatgttagcg atgttaaatt tgatcgtgac tcagctcatg gacttgttca tcctcttgat
     3661 ctcattgatg tactccaaga aaataccacc gatgacatga ctgttacagt tgatgtggga
     3721 agccattata tttggatggc tcgctacttt aaatcatatg aagcgcgtca cttgctcttc
     3781 tcaaacggaa tgcaaacatt aggagttgct ctaccttggg caatttctgc ggctcttttg
     3841 cgtcccaaca caaaagttat ttcagtttct ggtgatggtg gcttcttatt ctcagctcaa
     3901 gagttagaaa ctgcagttcg tcttcatcta ccaattgttc acattatttg gaacgatgga
     3961 aaatataaca tggttgaatt ccaggaagaa atgaagtatg ggcgttcttc aggtgttgac
     4021 tttggaccag ttgattttgt aaaatacgct gaaagtttcg gtgctaaagg ttaccgtgta
     4081 gacagcaaag acagttttga agaaacactc aaacaagctc taatcgatgc tgaaaatgga
     4141 ccagtgttga ttgatgtgcc tatcgactat aaagataatg tgacactagg tgaaacaatc
     4201 ttacccgacg agttctacta agacgaggtc taatatgtca gaaacagtta aattatttca
     4261 atacagtacc ttgagttcac tgatggcagg attgtacaaa ggcagtctaa ctatcggaga
     4321 attattaact cacggtgact taggaatcgg tactgttcat atgattgatg gtgaattgat
     4381 tgtattggat gggaaagctt atcaagctat tggtacagat ggtaaagcag agattatcca
     4441 attatcagat gatgtgacag taccctatgc agcggtttta ccacaccata tccaaaaaca
     4501 atttgatatc aatgctgaga cagataataa ggatttggaa gagatgattc taaaaaactt
     4561 tgaaggtcaa aatctcttta agtctttaaa aatcaaagga acgttttcaa gaatgcacgt
     4621 tcgtatgatt ccaaaatctc cacaacataa acgttttgca gatattgcat ctaatcagcc
     4681 agaatttacc aaagagaatg tatctggtac gttggttggg atttggacgc cggaattatt
     4741 tcatggtgtg ggggtaaaag gattccatgt ccactttatt tcagacgatt taactttcgg
     4801 tgggcatgtt atggattact cgctcacaca aggaaaagtt gaaattggga aggttgacca
     4861 acttgaccaa tgtttcccaa ctcaagatca agaatttttg gaagctaact ttgacctaca
     4921 aaagcttaga gaagatattg atttaagtga ataagaatac ctataaataa ttaataacac
     4981 taaaaataga cagggatatg tcccagtcta tttttttagt ttaaggctat ctattttttc
     5041 ttgtgtggga gtgactcgta aggttttttg accagtgtaa gttacaaaac ctggtttagt
     5101 accacttggt ttattgagtt tttttacttc aatcatgtct acttgaacaa gattggagag
     5161 acgagcttta gagtaataag cagctaactc ggcggcatct gtcttaacct cgtcacttgg
     5221 attgaggtta tcccgaatta acacatgact tccggggata tccttggcat gaaaccacaa
     5281 ctccccttta cgagccattt taaatgttaa ctcgtcattt tggagattat tacgtcctac
     5341 cataataata gtcttgccat cggatgctaa atattgttca ggttttttgc gcttatgttg
     5401 tttatcatgt gcgcgtcgct taataaatcc agtttcaaca agttcttcac gaatatcatt
     5461 gatatcttcc atagaagcat gatttagcga tgtttcaaca gattcaaggt aggtgattgt
     5521 attttccgta tctgaaataa ttccttttaa atgttttaca gcttctttta atttttgata
     5581 ttttttaaag tagcgttggg cattttggtt gggtgttaaa gctcgatcaa gtgaaatctc
     5641 aatcgtttgg ttggtgtaat aattatcaag cacaacgaca tcttgattat ttggtactat
     5701 agagagatag gtagttagaa gctctccttt ttggcgaaat tcctctgcat tttcagtagc
     5761 tagaagttcg tcttgttgct tagctagttt tttgatattt ttctctaact cactttgaac
     5821 acggtgaatg aggtcactag cttgctgtgc tatgcgatct ttttcagctt tctcttggta
     5881 gtaataatct aacaattgtc caagagactg aaatgtttcg tgactgcttg agaattggac
     5941 agcggaaaag gatttttctg ttaggctagg atgatattct ctcgaaaaga attgacgaaa
     6001 atcgttaagt ttattgtctt tcaaacagtt agataattct agcgcagtat cgcgtcctaa
     6061 accttggaag agttgttgga ggtttttagg gcttagatca ttggtttgaa gcagctcaaa
     6121 tagagtttga tcagaaatat caaaaggatt gattgctttt gttttgggtg gggcaatgta
     6181 agtcgaacca ggaaggatag tacggtagga gttttgcgag aaaccaacat gcttaattga
     6241 ttcaataatt ttatgttcat ttttatcaat caagataatg ttactgtgtt ttcccatgat
     6301 ttcaacaaca agtgtcgctt taatatgatc accgatttcg tttttattag acacgacgat
     6361 ttctaatata cggtcatttt caatttgttg aatggtttca atcactgcac cttgcaaata
     6421 ctttctcata atcattgtaa aagtatttgg attttgagga ttttggaaat ttgcttcggt
     6481 agtttggata cgtccaaata cagggtgagc agataggagt agtttatagt tgcgacgatt
     6541 attgcgtatt gttaatacca attcatggtc aaaaggttgg ttaatttttt ggatacgtcc
     6601 tttttcaatc tgttcttgta attcatttgt gaggtgatgt aaaaaaaatc catcaaaaga
     6661 catggcgtcc cccttattct aaaaattata gcttatttat tataccatag gctgagctaa
     6721 aaatattttt ttataacgca aaaggcttgc aattttcaga atatagtata taattataga
     6781 aaaattcatg agaaaggatg actagtaaat gactaaaaca ttatttacca attggcaaaa
     6841 ctggcgtaga taaagttgtg tttatgggtg catagatacg actttttgga gtacttcaaa
     6901 aaagtatcta tgcgctggtc tagtctaaca atagagagcg caaggtaagt tatttatcag
     6961 gcgctttttc ttttgcgcta aactccagaa agtcaaggta ttattaaagt gaggagaaaa
     7021 tatgaaaaat aaagggctga tagctacgct aattctattg actatattag tggttggaga
     7081 attattttat aacaaatcag aaaaacgtct taatttatca gaaaagcaag ttgttaaaat
     7141 tggtatttta cagtatgtaa cccatgatgc tttggatgct attgaaaagg gagttgaaga
     7201 tggtctagct caagaaggat ataaagggaa aaaagttaaa ttaaccgttt taaacgccga
     7261 agctgatcaa agtaaaattc aagcgatgag taaacaatta gttaatcatc acaatgatat
     7321 tttgattgga attgcgacac catcagcaca aggtttagca gcttcgacta aggacactcc
     7381 tataatcatg ggagctgttt ctgatccttt aggggccaag ttagtaacga atatgaagaa
     7441 accaacaact aatgttacag gattatcaaa cgttgttccg accaagcaaa ctgtgcaatt
     7501 aatcaaggat ataactccca atgttaaacg tataggaatt ctttatgcta gtagcgaaga
     7561 taattctgta tcacaagtaa ctgagtttac aaaatatgct caaaaagcag ggctagaagt
     7621 tcttaaatat tctgtaccat ctacaaatga aattaagaca agtatgtcag tcatgaccaa
     7681 aaaagtagac gctgtctttg ttccacaaga taatactatt gcgagtgctt ttagaacagt
     7741 tatcgtagca gcaaaccaag ctaatatacc tgtgtattcc agtgttgata ccatggttga
     7801 acaaggaagt atagcatcag tagcgcagag tcaatatggc ttagggttag aaactgcaaa
     7861 acaggccatt aaagtcctaa gagggaagcc ggttaaagat gtaccagtca aagttattga
     7921 tacagggaag ccaagcctca atttgaaagc agccaaacac ttaggaatta aaattcctaa
     7981 gaagattatg aaacaggcag aaattaccgt taaggttgac gattagaaag agggttttat
     8041 gattatttca tcagtttcac aaggtttatt atgggggata ttaggattag ggatatactt
     8101 aacctttaga atcttaaaat ttccagacat gacaacagag ggctccttcc ctctaggggg
     8161 agcggtttgt gttaccttaa tgaatcaagg ggttaatcct attttggcga cgattctagg
     8221 catgctatca gggatgttag cgggatttgt tactggttta ctttatacta aaggaaagat
     8281 accgacgatt ttagcaggga ttttagtaat gacctcatgc cactctatta tgctaatggt
     8341 tatgaaaaga gcaaatctag gtttaaatga gattcaaaca ttgaaagatt ttcttccgtt
     8401 ttcaaatgat ttaaatcttc tcgtattagg cttgatagct attctcttgg tgatttctgc
     8461 cttaatttat tttctctata cacgcttggg gcaagcttat attgcaacag gagataaccc
     8521 tgatatggca aagagcttcg gcattgatac cgacaaaatg gaaatgcttg gcttaattgt
     8581 atcaaatggc ttaattgctt tatcaggagc tttagtaagt caacaagatg gatatgctga
     8641 tgtatcaaaa ggtataggtg ttattgttat tgggttagct agtattatta ttggagaggt
     8701 actttactct actggtttaa ctctctttga acgtttaatt gctatagtgg tgggttctat
     8761 tctttatcaa tttttaataa cggcagtaat cgccttagga tttaatacga attacctaaa
     8821 attatttagt gcaatagtgc ttggaatttg tttaatgatt cctgtcctaa aaacgaagat
     8881 tttgaaagga gtacgcttat aatggtgatg aaaatcatag agttaaaaga agcaaccgta
     8941 caagtcagta atggtttagc agaaatgaaa acgatattag accatgttaa tttgagtatt
     9001 tatgaacatg acttcattac aatattgggt ggaaatggag ctggaaaatc aacacttttt
     9061 aatgtaattg caggtacctt gatgttaagt agtggaaata tttacatcat gggacaagat
     9121 gttactaatt taccagcaga aaaacgagct aaatatttat cacgagtatt tcaagatccg
     9181 aaaatgggaa cagcacctag gatgacagtt gctgaaaatt tattagttgc taaatttcga
     9241 ggtgaaaaga gaccgttagt tcctagaaag attattaact ataccgaaga atttcaaaaa
     9301 ttgattgcta gaactggtaa tggacttgat cgccatttag agacacctac aggtttatta
     9361 tcaggtggac aaagacaagc acttagttta ttaatggcaa ctttgaaaaa accaaatctg
     9421 ttattattgg acgaacacac agcagcgctt gatcctagaa caagtgtttc gcttatgggc
     9481 ttgactgatg agtttatcaa acaagattcg ttgactgcct taatgattac acatcatatg
     9541 gaagatgctc tcaaatatgg aaatcgtgta ttagtaatga aagatggcaa gattgtcaga
     9601 gatttaaatc aggcacaaaa aaacaagatg gctatagctg actattatca attatttgat
     9661 taaaaagaag tgaaatggaa gtgtttcact tctttttcta tacactttgc aagaaattat
     9721 tttcaaaata tgatatacta ataatgctgt aaatcgactt gtagagacaa ggaccgtaag
     9781 tacaaatggg atgaaaataa ggacacactc tttaattcat attcctatta ttgattaata
     9841 aggtttttct tcaaacccaa gaaaagttac caataaatca attactaatg ataataattg
     9901 gacaatttct tgtctgcttt ataaaaagaa ttaacaaaat ttaaggagaa aactcattat
     9961 gagcgacata aaaatcatgg ctcttggagg tgttcgtgaa aacggaaaaa acctatatgc
    10021 agtagaagtc aatgattcta tctttgtttt ggatgctgga ctcaaatatc ctgaaaatga
    10081 acaactgggt gttgatgttg ttattccaaa tcttgattac ttaattgaaa ataaaaaacg
    10141 tgttcagggg attttcttaa cccatggaca tgcggatgct attggtgcat tgccttatat
    10201 tatcgccgaa gtcaaagcac ctgtgtttgg ctcacctctc acaattgaat tagctaagtt
    10261 attcgttaaa aatagtacag cagtcaagaa attcaataat ttccatgtga ttgatagtga
    10321 aacggaaatt gaatttcaag atgcggtgat ttcgttcttt aaaacgactc attcaattcc
    10381 agagtcaatg ggaattgtta tagggacgaa agagggaaat atcgtttaca caggtgactt
    10441 taaatttgat caagctgcaa ggaaatatta ccaaacagat ctggcacgtt tagctgaaat
    10501 tggtcgtgat ggtgttttag cacttttatc agattctgca aatgctacaa gcaatgagca
    10561 agttgctagc gaatatgagg tcggtgatga aattaagagc gttattgaag acgcagaagg
    10621 tagagtcatc gtagcagcag ttgcaagtaa tctcattcgt attcaacaag tctttgatgc
    10681 agcggcagaa aacggtcgtc gagtagtttt aacgggtttt gatattgaaa atattgttcg
    10741 tacggccatt cgtatgaaac gcattcatat tgctgatgaa aatatgatta taaaacctaa
    10801 agatatgaca cgttacgaag ataacgaatt attaatcctt gaaactggac gtatgggaga
    10861 gccgataaat ggtctccaaa aaatggcgat tggacgtcat cgttatgttc aaattaaaga
    10921 tggtgattta gtatttatcg taacaacacc aagtattgca aaagaggcag ttgtagcgcg
    10981 tgttgaaaat ctgatttata aagctggtgg ctcagttaaa ttgataacac agaatttgcg
    11041 cgtatcaggt catgcaaatg gtcgcgaatt acaattatta atgaatctat tgaaacctaa
    11101 ataccttttc ccaattcaag gagagtatag ggatttgtca gcacatgcgg gcttagctca
    11161 agaggtagga atgtcagcag atgatattta tattgtgaag cgtggtgaca tcatggttct
    11221 tgaaaaagat ggcttcttcc atagtggatc tgttcctgct ggagatgtta tgattgatgg
    11281 aaatgctatt ggtgacgttg gtaatattgt acttcgtgac cgtaaggtat tatctgaaga
    11341 tggaattttt attgttgtta taactgtcag caaaaaagaa aagaaaatca tttctaaagc
    11401 tcgtgttaat acgcgtggtt ttgtgtatgt gaaaaaaagc agagatattt tgcgtgagag
    11461 cgcagaatta gttaatacaa cggtagaaga ttacttaagc aaagatacat ttgactgggg
    11521 tgaacttaaa gggaaagtgc gtgatgaggt cagcaagttc ttatttgacc aaacgaaacg
    11581 tcgcccagca atccttcctg tggttatgga agttcgttga tagtgttaag aggttaccaa
    11641 attttggtag cctttcttgc tagaatggag gtttttatgg cattttttaa tattgaatat
    11701 cactctaagg tactaggaac ggaacgtcaa gtcaatgtta tttacccaga tgcttttgag
    11761 atgcctgatg ataaaattga tgattgtgac attcctgttt tgtatcttct ccatggaatg
    11821 gggggaatga gaattcttgg caaaaacgca caaatataga gcgtctttta cgtcatacta
    11881 atcttatcgt cgtgatgcca tcaacagact tagcatggta taccaacaca aagtatgggt
    11941 tggattattt tgatgcaata gctatcgagt taccaaaagt tttgaaacgt ttctttccta
    12001 atatgtctga taagagagag aaaaatttca ttgctggatt atctatgggg ggatatggag
    12061 catataaaat agctctactg actaatcgtt tttctcatgc tgcctctttg tcaggtgcac
    12121 tttcttttga ctttgattta ttattcaata atggcaacga taacattaac tattggtcgg
    12181 gaatttttgg cgatttgaat aacactgata acatagagcg tcattcacta aggcgttacg
    12241 ttgagtcttt tgatatgaag actaaatttt atgcatggtg tggttatgaa gattttcttt
    12301 ttgaagctaa tgaagtcgca attgatgaac ttcgtcaatt aggtttaact attgactatt
    12361 ccaatgatca tggtaaacat gaatggtatt actggaatca acaattagaa aaagtcttag
    12421 aatggctccc tgttgattat gtcaaagaag agcgcctttc ttagtattag ctaaatcgtt
    12481 gaaatccaag taaagtcttg ttatactaaa tcattaagtt ttgggaaatt agacacagaa
    12541 ggtgagataa aaaatgagaa cactatttag aatgatattt gctattccaa agtttatctt
    12601 tagattggtt tggaatatca tttggggaat attcaagaca gttcttgtta ttgcgattat
    12661 tttatttggc ttgtattact atgcgaatca cagtcaatca gaatttgcta atcaacttag
    12721 tgacattatt cagacaggaa aaacattttt aaattttgct gacactaatc agttcaaaaa
    12781 ttcctttgct aatctagcta cggataacgt tcaccattca gaaaatacca gatgggataa
    12841 aaatcaggca actatctata tagcaactag agattctgag ttggtctctg cttacaagca
    12901 agcaatctcc aattggaatg caacaggtgc ttttgtgtta caaacaacag acaatcctaa
    12961 tgctgatatt attgctaagg attactcaga tgctaaaaca caagctgcgg gtgtagctga
    13021 aaccgagaaa aatgctttaa ctaatcgtat ttcaaaggta acagttaaat taaacacatt
    13081 ttaccttaag aataaacagt ttggttatga ccatactagg attgttaata cggccgaaca
    13141 tgaattagga catgctttag gtctaggaca taatgatcaa caacattccg ttatgcagtc
    13201 aaaaggttcg cattatggca ttcaagaagt tgatattcaa acgctaaaaa acttatatgc
    13261 ataaaaagat gagctaagaa actcatcttt tttgtttgaa cacttatttt aggtgatata
    13321 agtgcgttag caatagatta ttaaaaataa agaatttgat tatcactact aactaaaatg
    13381 tataaagaac aaagaaaatg ttttcaaaaa tcacttaaaa aatggtataa tgtgtcttga
    13441 gatagtgttt tgaatagact tatcaaaaat attagacaac attaacttag aaggggaaaa
    13501 tatggacgaa ctaaaaaaac ttgctggagt gaccgctgca aaatacgtta aaaacggtat
    13561 gattgtaggt ttagggacag gttctacagc atattttttt gttgaagaaa ttggacgccg
    13621 tgtcaaagaa gagggattgc aagtcgtagg cgttacaacg tcaaaccgta cgactgagca
    13681 ggcaagagga ttaggtattc cgttgaaatc agcagatgat attgatgtca ttgatgtcac
    13741 tgtggatgga gcagatgaag tagaccctga ttttaatggt atcaagggtg gcggtggtgc
    13801 gctgctcatg gaaaaaatag tagctactcc aactaaagaa tatatctggg ttgtcgatga
    13861 gagcaaactt gttgaaacgt taggtgcatt taaattgcct gttgaggttg ttagatatgg
    13921 ttctgagcgt ctttttcggg tttttaaatc aaaaggttac tgtccttctt tccgtgaaac
    13981 agaaggtgac cgctttatca ctgatatggg gaactacatt atcgacctag atttgaaaaa
    14041 aatagaggat ccaaagcagt tagcgaatga acttgaccat acagttggtg ttgttgaaca
    14101 tggtttattt aatggtatgg ttaataaagt tatagtagca ggcaaaaacg gtcttgacat
    14161 acttgaaaaa taattattta aataatcggt tttaggagct aaccgtaacc tccttagaaa
    14221 gaagatataa tgtcacaatt tgatcgcatt cacttagttg tcttagattc agtggggatt
    14281 ggagcggcac cagatgccaa tgattttgtc aatgcaggag ttcctgatgg tgcttcagac
    14341 acgcttggcc acatttcaaa aacagttgga cttgcggtgc caaacatggc taaaatcgga
    14401 cttggtaata ttcctcgtcc ccaagcgctt aagaccgtcc ctgcagaaga aaatccaagt
    14461 ggctacgcga cgaagttaca agaagtatca cttggtaaag atacaatgac agggcattgg
    14521 gagatcatgg gcttaaatat tacagagcca tttgatactt tctggaatgg ttttccagaa
    14581 gatattatta caaagattga ggatttttca ggacgtaaag ttattcgtga agcgaataaa
    14641 ccttattcag ggacagctgt tattgatgac ttcggaccac gccaaatgga aactggtgag
    14701 ctcatcatct atacctcagc tgatccagtc ctacaaattg cagctcatga ggatatcatt
    14761 cctctagaag aactctaccg tatttgtgaa tatgctcgta gtatcacaat ggagcgtcca
    14821 gctctccttg gtcgtattat cgctcgtcca tatgtaggtg aaccaggcaa ctttacgcgt
    14881 acagccaacc gtcacgacta tgcagtatca ccatttgaag ataccgtatt aaataaacta
    14941 gatcaagctg gcatcgatac ttatgctgtt ggtaaaatca atgatatttt caatggttca
    15001 ggcattaatc atgatatggg acataacaaa tcaaatagtc atggtattga tactttgata
    15061 aaaacaatgg ggttatcaga gtttgaaaaa ggcttctcct ttactaattt ggttgacttt
    15121 gatgctctct atggtcaccg tcgtgatcct catggttatc gtgattgtct tcatgagttt
    15181 gatgagcgtc ttccagagat catttcagcg atgcgtgaca aagacttact tttgatcact
    15241 gccgatcatg gtaatgaccc aacttatgca ggtacagacc atacaagaga atacattcct
    15301 ctcttagcct atagcccatc tttcacagga aacggcctta tcccagtagg gcatttcgca
    15361 gatatttcag caacagttgc ggataacttt ggtgttgata ctgcgatgat tggagagagc
    15421 ttcttgcagg acttggtata aatttttatt tccttaagac acttgtagca ttaaaaattg
    15481 agaggaagat gatggaaaaa attaggattt accataaccc aaattgtggg acatcaagga
    15541 acgtacttgc cattattcgt cattgtggta ttgaaccaga aattatttat tatctgaaaa
    15601 caccacctag caggatggaa ctggttgaac tgttgttaga aatgaaactt tctgcgaggg
    15661 aacttctaag aacagatgtt cctgcttatg agaaattcaa tttggaatct tctagcgtta
    15721 cagatgaaga aatgattgat gcgatgatac aagatcctat tcttatcaat cgtcccatag
    15781 ttgttacgag taaaggtgca aaactctgcc gaccctgtga agcaatttta accattttac
    15841 ctgtaaagat ggaaaaagat tttgttaaag aagatggaca gattatccaa agtttatagc
    15901 atattgtata gtgaataatg gtttaggagg taagtaaatg acattattag aaaaaattaa
    15961 tgagactaga gactttttgc aagcaaaagg cgtcacagca ccagaattcg gccttatttt
    16021 aggctctggt ttaggagaat tagctgaaga aatcgaaaat cccattgttg tggattatgc
    16081 agacatccca aattggggac agtcaacagt agttggtcat gctggaaaat tagtgtatgg
    16141 ggaactatca ggccgtaagg tattagcgct tcaaggtcgt tttcactttt atgaaggaaa
    16201 tacaatggaa gtcgttactt tcccagtacg tatcatgaga gcattggctt gccacagtgt
    16261 gcttgtgact aatgcagcag gtgggattgg atacggacca ggaactttaa tgctgatcaa
    16321 agaccacatc aatatgattg ggactaaccc tctcataggt gagaaccttg aagaatttgg
    16381 accacgtttc ccagacatgt cggatgctta tacagcaaca tatcgacaaa aagctcacca
    16441 aattgctgaa aaacagaata tcaaactcga agaaggtgtg tacttgggtg tatcaggacc
    16501 cacttatgaa acacctgcag aaattcgtgc attccaaaca atgggcgcac aagcggtagg
    16561 tatgtccacg gttccagagg tgattgttgc agctcactca gggcttaaag tgttaggaat
    16621 ttcagcaatt actaactttg ccgctggttt ccaatctgag ctcaatcatg aggaggtcgt
    16681 tgaagttact cagcgtatta aagaagattt caagggatta gttaaatcat tagttgctga
    16741 actttaaaat ggtttctcga ttatactatg ctgtcaagtt tatgatagcc gttctattta
    16801 tgacagttat ggcaggagtt ggagctatct taatgcacta tgtattaatg tttacagagt
    16861 ggttagcatt tggtgatagt agagaaaata ctctgtcttt gttaaatagt gtcacaccaa
    16921 ttaaaagagt gttatcactt actttagtct cctttttagc atcccttagt tggtattact
    16981 tacaaatcaa accaaagcaa ataactagta ttaaacaaca ggttgttttt aaggattctt
    17041 ctgtaaaaaa atcaccttac tggctacata taggtcacgc atttttgcaa ttgatttatg
    17101 taggagctgg tggtcctatt ggaaaagaag gagcgccacg agagtttgga gccataaatg
    17161 ctgggaaaat atcggattta ttagcactta aagtgctgga taaacgttta ttaatcatta
    17221 gtggtgcagc tgcaggtttg tcagcagttt atcaagtacc cctagctagt gtattttttg
    17281 cttttgaaac tttagcttta ggaatctcac tgaaaaatat tgtgacattg ctagcctcta
    17341 cttttggtgc agctagtatt gctcaattag ttatttcgac agcccctctt tatcatattt
    17401 caaagatgag tctgaattca cagtcattag cttttatgtt cttgatcgtt ttatgtgtaa
    17461 ctccgattgc gattagcttt agatatttaa atcaaaaagt gacagagaga cgtattaaaa
    17521 atataaaaat attgttaagt ttaccagtag tatcattgat agtatctgtt ttatcaatag
    17581 tctatcccca gattttagga aatggtaatg cactagtcca agaagtcttt aaagggacct
    17641 cagttagctt aatcgctatt ttagttgttc tcaagatgat agcaacatta tccactcttt
    17701 acgctggtgc ttatggcggt atactgacac catctttttc cattggtgct tgtttaggtt
    17761 ttttgttagc tagtatttct attcctttac ttccccacat atcgatagta accagtatgt
    17821 tggttggtgc agctattttt ttagctatta caatgagagc gccactgaca gctgtcggtt
    17881 tagtcatatc ttttactggt caatccgtta taactattgt tcccttaact atagcagtac
    17941 tttttgctac tgcctatgat tattttataa gaaaaatgag gagtttatat gtcaatccat
    18001 attgaagcaa aacaaggtga aattgcagat aaaattttac ttccaggaga tccactccgt
    18061 gcgaaattta ttgccgagaa ttttttagaa gatgcggtgt gttttaatac agttcgcaat
    18121 atgtttggtt atacaggtac ctataaagga catcgtgttt ctgttatggg aactggtatg
    18181 ggtatgcctt caatttcaat ttatgctcgc gaactaattg ttgattatgg ggttaaaaca
    18241 ttgattcgcg taggaacggc aggtgctatt aatcctgata tccatgttcg tgaacttgtt
    18301 ttagcacaag cagcagcaac aaattctaat attatccgta atgattggcc tgaatttgat
    18361 tttccgcaaa ttgctgattt taaacttttg gataaagctt atcacatcgc aaaggaaatg
    18421 gatattacaa ctcatgtagg cagtgtatta tcttcagacg tgttttattc aaaccaacca
    18481 gatcgtaata tggccttggg taaactaggt gttcatgcta ttgagatgga agcagcagct
    18541 ctctactatc ttgcagctca acataatgtt aatgctcttg ctatgatgac gatttcagat
    18601 aacttgaata atccagaaga agatacaagt gcagaagagc gtcaaacaac atttactgat
    18661 atgatgaaag ttgggctcga aacacttatt tctgaatgag gatagaaatg acagattttt
    18721 caactgctct taaagtatta gtggatcagt attcttatca taatgctttc ttattattac
    18781 aaaaacatgg tcctttaaac tcagatttat tatttttatt ggaaatgatg aaagagcgtc
    18841 gagagttaaa cattgatttt ctttttgctc atcaagaaca agttgttata ttacaagaaa
    18901 aatacaatat aaagcttttg cataacccgt atgatttaga gttgctggca aattacatca
    18961 tggatttaga agcaaaagta aaaaatggat taatcataga ttttgtccgt agtgttagtc
    19021 ctattttata tcgtctattt atgattttac ttgcacaaga agtacctcac ttgcatgatt
    19081 acattcataa tgcaagagat gatcactacg atacttggaa gtttaaagaa ttaaaggagt
    19141 caaaccaccc agtccttttg gcattctctg aaaggtggca cgatagtcgc ttgacttcta
    19201 aaagccttgc agaatgttta caattaaccg acctggatga agaagtgaaa tcgaccatca
    19261 ttcaattaag acagttcgaa aaatcagtca gaaatccttt ggctcacctg attaaacctt
    19321 ttgatgagca agaactatat cgtacaactc aattttcttc tcaagcattt ttagaccaga
    19381 ttatcttctt ggcaaaggta attggtgttg agtatgatac tgttaatttt cactacgata
    19441 cggttaacaa gcttattata aagatacttg agtaatttaa aaacagttct gagacaatct
    19501 caggactgtt tgtttttatg attaaacgaa acttcctcta ataaatagtc aataaatttt
    19561 tgtcccattt ttgaaaggtt tgctttgtcg tgtcggatat agacaatatc aataatatca
    19621 tccacgtcaa gagggattgc gacaatttcg tcaccgttca attttgagtt gaggatacca
    19681 gttgccactg tataaccatc taatccaatc attaaattaa acagagttgc tctatcactg
    19741 acaacaatgg attttgggtg agtaatttga gacatcattt cctcagaaaa gtagaaagaa
    19801 ttatgcaggc cttgatcata actcagataa ggataatctt ctaaatcttt catacttaat
    19861 ttttttctat tagctaaggg attggatttg ctcacgaaaa tatgaggtgt agtagtaaaa
    19921 agagttgtgg caattaagga attatcgtcg aataatttgg ttaatacatc tctattatag
    19981 ctattaagaa acagaacacc aatctctgaa cggaaatttt taacatcatc aataatttcc
    20041 caagtcctag tttcacgtaa aaaaagttcg tattgggtca tgtctgtgcc attaaagaga
    20101 gctacaaaag cgttaaccac aaatgcatag tgttgtgaag aaacgctgaa cagctcacga
    20161 cttgtattat cacccttata acgctcttca agaagtgctg tttgttctag aatttgtcgc
    20221 gcatatgaca gaaattccat accatctttt gttaacgtta ttccttttgg atttcgaata
    20281 aaaatttgga tacccatctc agtctcaagg ttcctaacag cgtttgaaag tgaaggttgg
    20341 gtaatgtaga gttgtttagc ggcttcattc atgctaccag tttctacaat cttaataaca
    20401 tattgtaatt gttgaattct cataactcta gtttaaacta gaatgtaaat aaattcaatt
    20461 agatcagcta aattatcaat atagatcaat tgaaaatgtt at
//
LOCUS       DATRNK010000035        20197 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.035, whole genome shotgun sequence.
ACCESSION   DATRNK010000035 DATRNK010000000
VERSION     DATRNK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20197
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.035"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            230..301
                     /locus_tag="VBC52_001244"
     tRNA            230..301
                     /locus_tag="VBC52_001244"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:262..264,aa:Gln,seq:ttg)
     gene            310..381
                     /locus_tag="VBC52_001245"
     tRNA            310..381
                     /locus_tag="VBC52_001245"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:343..345,aa:Glu,seq:ttc)
     gene            444..962
                     /gene="rimM"
                     /locus_tag="VBC52_001246"
     CDS             444..962
                     /gene="rimM"
                     /locus_tag="VBC52_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEO6882833.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVTDFVEERFKKGQVLALFDEK
                     NQFVMDIEIASHCKQKNFDIIKFKGMYHINDIEKYKGFTLKVAEDQLSDLKDGEFYYH
                     EIIGLDVYEGEELIGKIKEILQPGANDVWVVERHGKRDLLLPYIPPVVLEVDLSNQRV
                     QVELMEGLDDED"
     gene            949..1704
                     /gene="trmD"
                     /locus_tag="VBC52_001247"
     CDS             949..1704
                     /gene="trmD"
                     /locus_tag="VBC52_001247"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEO6882834.1"
                     /translation="MMKIDILTLFPEMFAPLEHSIVGKAKERGLLEINYHNFRENAEK
                     SRHVDDEPYGGGQGMLLRAQPIFDTIDKIDAQKARVILLDPAGRTFDQDFAEELSKED
                     ELIFICGHYEGYDERIKSLVTDEVSLGDFVLTGGELAAMTMVDATVRLIPEVIGKETS
                     HQDDSFSSGLLEYPQYTRPYDYLGMTVPDVLMSGHHENIRKWRLEQSLRKTLERRPDL
                     LENYAMTDEERLILEKIKTEIERTDTVNEQNNL"
     gene            1685..2677
                     /locus_tag="VBC52_001248"
     CDS             1685..2677
                     /locus_tag="VBC52_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6882835.1"
                     /translation="MNKTIYDITIVGGGPVGLFAAFYAGLRGVSVKIIESLSELGGQP
                     AILYPEKKIYDIPGYPVITGRELIDKHIEQLERFKDSIKICLKEEVLSFEKVDDVFTI
                     QTDKDQHLSRAIVFACGNGAFAPRLLGLENEENYADNNLFYNVTKLEQFAGKHVVICG
                     GGDSAVDWANELDKIAASVAIVHRRDAFRAHEHSVDILKASGVRILTPYVPICLNGDS
                     QRVSSLVVQKVKGDEVIELPLDNLIVSFGFSTSNKNLRYWNLDYKRSSINVSSLFETT
                     QEGVYAIGDAANYPGKVELIATGYGEAPVAINQAINYIYPDRDNRVVHSTSLIK"
     gene            2814..3884
                     /locus_tag="VBC52_001249"
     CDS             2814..3884
                     /locus_tag="VBC52_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO6882836.1"
                     /translation="MTNTVTPKETAGSFINKVLGGTATAIVVALIPNAILATFLKPFL
                     SYGLAAEFLHIVQVFQFFTPIMAGFLIGQQFKFTPMQQLAVGGAAYIGSGAWAYTEVI
                     QKGVATGSFQLRGIGDLINMMLTAALAVLAVKWFGNKFGSLTIILLPIIIGTGVGYLG
                     WKLLPYVSYVTTLIGQGINSFTTLQPIAMSILIAMAFSMLIVSPISTVAIGLAIGLNG
                     MSASAASMGVASTTAVLVWATMKANKSGVPIAIALGAMKMMMPNFLKHPVMAIPMLMT
                     ATVSSLTVPLFKLVGTPASSGFGLVGAVGPIASFEAGASMLIVILSWLVIPFAVGFVS
                     HKICKDILKLYKDDIFVFEGQN"
     gene            3897..4820
                     /locus_tag="VBC52_001250"
     CDS             3897..4820
                     /locus_tag="VBC52_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEO6882837.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNHDVILLDNWADHIMAIKENGL
                     KVTGDTEDLVKLPIMKPTDATEEADLIILFTKAMQLPNMLQDIKKIIGKKTKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLKGPGHAHLEGVGSVNLQSIDPNNQEAGHRV
                     TELLNEAKLQATYDENVLPNIWRKACVNGTMNSTCALLDCTIGQLFASEDGVNMVHEI
                     IHEFVTVGKAEGVELDEEEITKYVMDTSVKAAHHYPSMHQDLVQNQRLTEIDFLNGAV
                     NKKGENLGIDTPYCRLITQLIHTKENVLSIK"
     gene            5032..6666
                     /locus_tag="VBC52_001251"
     CDS             5032..6666
                     /locus_tag="VBC52_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEO6882838.1"
                     /translation="MMKKGQVNDTKQSYSLRKYKFGLASVILGSFIMVTSPVFADQTT
                     SVQVNNQTGTSVDANNSSNETSASSVITSNNDSVQASDKVVNSQNTATKDITTPLVET
                     KPMVEKTLPEQGNYVYSKETEVKNTPSKSAPVAFYAKKGDKVFYDQVFNKDNVKWISY
                     KSFCGVRRYAAIESLDPSGGSETKAPTPVTNSGSNNQEKIATQGNYTFSHKVEVKNEA
                     KVASPTQFTLDKGDRIFYDQILTIEGNQWLSYKSFNGVRRFVLLGKASSVEKTEDKEK
                     VSPQPQARITKTGRLTISNETTTGFDILITNIKDDNGIAAVKVPVWTEQGGQDDIKWY
                     TAVTTGDGNYKVAVSFADHKNEKGLYNIHLYYQEASGTLVGVTGTKVTVAGTNSSQEP
                     IENGLAKTGVYNIIGSTEVKNEAKISSQTQFTLEKGDKINYDQVLTADGYQWISYKSY
                     SGVRRYIPVKKLTTSSEKAKDEATKPTSYPNLPKTGTYTFTKTVDVKSQPKVSSPVEF
                     NFQKGEKIHYDQVLVVDGHQWISYKSYSGIRRYIEI"
     gene            6810..8045
                     /locus_tag="VBC52_001252"
     CDS             6810..8045
                     /locus_tag="VBC52_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6882839.1"
                     /translation="MTLRELTIEEFKEHSGNYDSQSFLQTPEMAKLLEKRGYDVRYLG
                     YQVENKLEIISLSYIMPVTGGFQMKIDSGPVHSNSKYLKQFYKALQGYAKSNGVLELI
                     VEPYDDYQLFTSSGVPSNQGNDNLIEDFTSSGYHHDGLTTGFTGKYLSWHYVKNLEGV
                     TSETLLSSFSKTGRALVKKAMSFGIKVRVLKRDELHLFKEITTSTSNRRDYMDKSLDY
                     YQDFYDSFEGKAEFVIATLNFREYDHNLQIKAEALENKLKLLDERFRENADSPKYHRQ
                     RSEIINQLASFETRRQEVQSFIQKYDNQDVVLAGSLFVYSLKETVYFFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRGSTFYNLLGIQGTFDGSDSILRFKQNFNGYIIRKMGTFNYY
                     PSPFKYKGIQLLKKVLKRN"
     gene            8110..8919
                     /locus_tag="VBC52_001253"
     CDS             8110..8919
                     /locus_tag="VBC52_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6882840.1"
                     /translation="MLDIERKRDIIKLQTISIQGGTMLKSKRKEIILSRLEQNKSVTL
                     DELTSILETSESTVRRDLDELESEGFLKRVHGGAELPYSLGQELSNQEKAIKNVQKKL
                     DIARQTAKLIAKQDVIFIDAGTTTELLIDFLPHEQLTVVTNSIHHAAKLVDRGIKTII
                     IGGAVKHSTDASIGQVAINQIRQITVDKAFLGMNGIDEVYLTTPDLEEAAIKEAIINN
                     SQQTFILMDSSKIGQVTFAKVKEINDINLVTNKTDSELMTIIKEKMKVIQV"
     gene            8916..9827
                     /gene="pfkB"
                     /locus_tag="VBC52_001254"
     CDS             8916..9827
                     /gene="pfkB"
                     /locus_tag="VBC52_001254"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEO6882841.1"
                     /translation="MIYTVTLNPSIDFIVRLDTLLLGSVNRMTSDDKYVGGKGINVSR
                     ILKRLKIDNTATGFIGGFTGHFVEDGLVSEGIKTDFVSVNEDTRINVKVKAKIETEIN
                     GGGPRITNEQLHRLEKLLSRLTPEDTVVFAGSAPASLGNKVYNTLIPIAKKTGAEVVC
                     DFEGQTLLDALAYQPLLVKPNNHELADIFGVELEGLPDIEKYAHKILDKGAKNVIVSM
                     AGDGALLVTPEASYFAKPIKGEVKNSVGAGDSMVAGFTGEFVKSKNPVEALKWGVACG
                     TATTFSDDLATAEFIQDIYNKVEVEKL"
     gene            9824..11788
                     /locus_tag="VBC52_001255"
     CDS             9824..11788
                     /locus_tag="VBC52_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6882842.1"
                     /translation="MKIQDLLKKEVMIMDLKATSKEAAIDEMITKLVDTGVVTNFAIF
                     KDGIMKREAQTSTGLGDGIAMPHSKNAAVKEATVLFAKSASGVDYEALDGQPTDLFFM
                     IAAPDGANDTHLAALAELSKYLLKEGFADQLRQAKTPDDIIATFDSNSISQETVAPQT
                     VQSTSKGSDYIVAVTACTTGIAHTYMAEEALKKKAAEMGVGIKVETNGASGVGNKLTS
                     SDIARAKGVIIAADKAVEMDRFDGKPLVSRPVADGIKKSEDLINIILDNKAQTYHAKN
                     QNDKQSGESDGKSGLGSAFYKHLMGGVSQMLPFVIGGGIMIAIAFLFDNILGVPKDQL
                     SNLGSYHEIAALFKNIGGAAFAFMLPVLAGYIAYSIAEKPGLVAGFVAGSIASSGLAF
                     GKVPFAEGGKATLALAGVPSGFLGALVGGFLAGGVILLLRKLLSGLPKSLEGIKSILL
                     YPLLGVLITGFLMLLVNIPMAAINTALNTFLQGLSGSSAVLMGLLVGGMMAVDMGGPV
                     NKAAYVFGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFNNEERQSGL
                     TNIVMGLSFITEGAIPFGAADPARAIPSFIVGSALTGALVGLAGIKLMAPHGGIFVIA
                     LTSNPLLYILFILIGAVVSGVLFGLFRKTK"
     gene            11977..12471
                     /locus_tag="VBC52_001256"
     CDS             11977..12471
                     /locus_tag="VBC52_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEO6882843.1"
                     /translation="MIKWNEKETKLVDTSFSIMLYLKTLFAVGQVVAGVLLYYFKPTQ
                     IKHLLFLLTEGEVHGGIHGFISAHFLKISSYINDSASHLAAIYLLIHGGIKLVALILL
                     HKEKLFAYPLSILVFVGFIIFQLREYFSSHSILMIFLCVFDVIMIILTWLEYQKMKLN
                     FSKR"
     gene            12522..13055
                     /locus_tag="VBC52_001257"
     CDS             12522..13055
                     /locus_tag="VBC52_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882844.1"
                     /translation="MTMKTIEEIYKNYPNIPYISPERDLAEINFSKVVPRKNMEETSE
                     GLLPGDIILLWRIQFGTFTTETSFSKYFEYIYGINGKEHLEFLIKNGFVRMESPLDSL
                     DHLSAPLLKLFLKEKNVKGLSKMKRSDLDQAIAIAFTEEELGKLFVVRGLALTEKGLA
                     ALSNNQEVIDRHPKKKF"
     gene            13127..13507
                     /locus_tag="VBC52_001258"
     CDS             13127..13507
                     /locus_tag="VBC52_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEO6882845.1"
                     /translation="MEVIREQEFVNQYHYDARNLEWEEENGTPKTNFEVTFQLANRDE
                     AAKVTSIVAVLQFVIVRDEFVISGVISQMAHIQGRLINEPSEFSQDEVENLAAPLLEI
                     VKRLTYEVTEIALDRPGVTLEFNS"
     gene            13507..14355
                     /locus_tag="VBC52_001259"
     CDS             13507..14355
                     /locus_tag="VBC52_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6882846.1"
                     /translation="MKLALITDTSAYLPEAIENHEDVYVLDIPIIIDGKTYIEGQNLT
                     LDQYYDKLAASKELPKTSQPSLAELDDLLCQLEKEGYTHVLGLFIAAGISGFWQNIQF
                     LIEEHPNLTIAFPDTKITSAPQGNLVRNALMCSREGMDFDVIVNKIQSQIEKIEGFIV
                     VNDLNHLVKGGRLSNGSAIIGNLLSIKPVLHFNEEGKIVVYEKVRTEKKALKRLAEIV
                     KEMTADGEYDIAIIHSRAQDKAEQLYNLLAKAGLKDDLEIVSFGGVIATHLGEGAVAF
                     GITPKN"
     gene            14540..15748
                     /locus_tag="VBC52_001260"
     CDS             14540..15748
                     /locus_tag="VBC52_001260"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEO6882847.1"
                     /translation="MRLNYLPSEFQKALPILKKIKKAGYEAYFVGGSVRDVLLDRPIH
                     DVDIATSSYPEETKQIFKRTVDVGIEHGTVLVLEKGGEYEITTFRTEEVYVDYRRPSQ
                     VNFVRSLEEDLKRRDFTVNAFALNEDGEVIDLFHGLDDLDNHLLRAVGLASERFNEDA
                     LRIMRGLRFSASLNFDIETTTFEAMKKHASLLEKISVERSFIEFDKLLLAPYWRKGML
                     ALIDSHAFNYLPCLKNRELQLSAFLSQLDKDFLFETSEQAWASLILSMEVEHTKTFLK
                     KWKTSTHFQKDVEHIVDVYRIREQMGLTKEHLYRYGKTIIKQAEGIRKARGLMVDFEK
                     IEQLDSELAIHDRHEIVVNGGTLIKKLGIKPGPQMGDIISQIELAIVLGQLINEEEAI
                     LHFVKQYLMD"
     gene            15759..17627
                     /locus_tag="VBC52_001261"
     CDS             15759..17627
                     /locus_tag="VBC52_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6882848.1"
                     /translation="MSDFLVDGLTKSVGDKTVFSNVSFIIHSLDRIGIIGVNGTGKTT
                     LLDVISGELGFDGDRSPFSSANDYKIAYLKQEPDFDDSQTILDTVLSSDLREMALIKE
                     YELLLNHYEESKQSRLEKVMAEMDSLDAWSIESEVKTVLSKLGITDLQLSVGELSGGL
                     RRRVQLAQVLLNDADLLLLDEPTNHLDIDTIAWLTNFLKNSKKTVLFITHDRYFLDNV
                     ATRIFELDKAQITEYQGNYQDYVRLRAEQDERDAASLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFQNLKNDLHQTSDTSDLEMTFETSRIGKKVINFENVSFSYPDKSILKD
                     FNLLIQNKDRIGIVGDNGVGKSTLLNLIVQDLQPDSGNVSIGETIRVGYFSQQLHNMD
                     GSKRVINYLQEVADEVKTSVGTTSVTELLEQFLFPRSTHGTQIAKLSGGEKKRLYLLK
                     ILIEKPNVLLLDEPTNDLDIATLTVLENFLQGFGGPVITVSHDRYFLDKVANKIIAFE
                     DNDIREFFGNYTDYLDEKAFNEQNNEVISKKESTKTSREKQSRKRMSYFEKQEWATIE
                     DDIMILENTITRIENDMQTCGSDFTRLSDLQKELDAKNEALLEKYDRYEYLSELDT"
     gene            17624..18097
                     /locus_tag="VBC52_001262"
     CDS             17624..18097
                     /locus_tag="VBC52_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6882849.1"
                     /translation="MIIRPIIKNDDQAVAQLIRQSLRAYDLDKPDTAYSDPHLDHLTS
                     YYEKIEKSGFFVIEERDEIIGCGGFGPLKNLIAEMQKVYIAERFRGKGLATDLVKMIE
                     VEARKIGYRQLYLETASTLSRATAVYKHMGYCALSQPIANDQGHTAMDIWMIKDL"
     gene            18164..19903
                     /locus_tag="VBC52_001263"
     CDS             18164..19903
                     /locus_tag="VBC52_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6882850.1"
                     /translation="MAYIWSYLKRYPNWLWLDLLGAMLFVTVILGMPTALAGMIDNGV
                     TKGDRTGVYLWTFIMFIFVVLGIIGRITMAYASSRLTTTMIRDMRNDMYAKLQEYSHH
                     EYEQIGVSSLVTRMTSDTFVLMQFAEMSLRLGLVTPMVMIFSVVMILITSPSLAWLVA
                     VAMPLLVGVVLYVAIKTKPLSERQQTMLDKINQYVRENLTGLRVVRAFARENFQSQKF
                     QVANQRYTDTSTGLFKLTGLTEPLFVQIIIAMIVAIVWFALDPLQRGAIKIGDLVAFI
                     EYSFHALFSFLLFANLFTMYPRMVVSSHRIREVMDMPISINPNAEGVTDTKLKGHLEF
                     DNVTFAYPGETESPVLHDISFKAKPGETIAFIGSTGSGKSSLVNLIPRFYDVTLGKIL
                     VDGVDVRDYNLKSLRQKIGFIPQKALLFTGTIGENLKYGKADATIDDLRQAVDISQAK
                     EFIESHQEAFETHLAEGGSNLSGGQKQRLSIARAVVKDPDLYIFDDSFSALDYKTDAT
                     LRARLKEVTGDSTVLIVAQRVGTIMDADQIIVLDEGEIVGRGTHAQLIENNAIYREIA
                     ESQLKNQNLSEGE"
     gene            19908..>20197
                     /locus_tag="VBC52_001264"
     CDS             19908..>20197
                     /locus_tag="VBC52_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6882851.1"
                     /translation="MRKKSVFLRLWSYLTRYKATLFLAIFLKVLSSFMSVLEPFILGL
                     AITELTANLVDMAKGVSGAELNVPYIAGILIIYFFRGVFYELGSYGSNYFMTT"
BASE COUNT         6687 a         2961 c         4108 g         6441 t
ORIGIN      
        1 atatattaga aattgggatg aataataagc agtaccatga actactttta caagctatta
       61 tcgaaaaaag ggaccaaaaa gtttttgaat atatttcaaa aatgaaacat aaaatacttt
      121 gatagactct atatgagctt tattaaaagg gataaaatcg aaatttaaaa tttaattcga
      181 aaagatattg acagtaatct agaatttcga tatactagat tttgtctttt ggggtatagc
      241 caagcggtaa ggcaagggac tttgactccc tcatgcgttg gttcgaatcc agctacccca
      301 gtttcacatg gtccgttggt caaggggtta agacaccgcc ttttcacggc ggtaacacgg
      361 gttcgaatcc cgtacggact atcgtggcca gttgcatttt agcggctggt ttttctttta
      421 ttttcaaaaa tatcggagtt actatggaat actttaacgt tggaaaaatt gtcaatacac
      481 aaggattaca aggcgaaatg cgtgtattaa gcgtgactga ctttgtagaa gaacgcttta
      541 aaaaaggtca agtgttagct ttattcgatg aaaagaatca gtttgttatg gatattgaaa
      601 ttgctagtca ttgtaagcag aagaattttg acattataaa attcaaagga atgtatcata
      661 ttaatgatat tgaaaaatat aaaggtttca cactgaaggt ggcagaggat caactgagcg
      721 accttaaaga tggtgaattt tattatcatg aaattattgg cttagatgtc tatgaagggg
      781 aagagctgat tggaaaaatc aaagaaattt tacaaccagg agcaaatgac gtttgggttg
      841 tggaacgtca tggaaaacgt gatcttttat taccctatat cccgccagta gtattagaag
      901 tggacctttc taatcagcgt gttcaagttg aattgatgga aggtttagat gatgaagatt
      961 gatattttaa ctttatttcc tgaaatgttt gcccctctgg aacattctat agttggtaag
     1021 gcaaaagaaa gaggacttct tgaaattaat tatcacaatt tcagagaaaa tgctgagaag
     1081 tctcgacatg ttgatgatga gccttacggt ggggggcaag ggatgctttt acgtgctcaa
     1141 cctatttttg atacaattga taaaatagat gctcaaaaag cacgtgttat tttgctagac
     1201 ccagcgggca gaacatttga tcaggatttt gcagaagaac tatccaaaga agatgaatta
     1261 atttttattt gtggtcacta tgaaggttat gatgaacgta taaaatcatt agtaacagac
     1321 gaagtatctt tgggagattt tgttcttacg ggaggagaat tggcggctat gacaatggtc
     1381 gatgctaccg ttcgtctgat tccagaagtt ataggtaaag aaacaagtca tcaggatgat
     1441 tcattttcat cgggcctatt agaatatcct cagtatacgc gcccatatga ttatttaggc
     1501 atgacagttc ctgatgttct catgagtggt catcatgaaa atatccgaaa atggcgttta
     1561 gaacaaagtt tgcgaaaaac actagaacgt cgaccagatt tgttagaaaa ttatgcaatg
     1621 acggatgaag agagacttat cttagagaaa attaaaacag aaatagaaag aacagataca
     1681 gtaaatgaac aaaacaattt atgatatcac tattgtagga ggcggaccag taggtctctt
     1741 tgctgctttt tatgctggac tacgcggagt atccgttaaa attattgaaa gcctttcgga
     1801 attaggaggc cagccagcta tcctttatcc tgagaaaaaa atctatgata ttcctggtta
     1861 tccggtgatt actggacgtg aattaattga taaacatatt gaacaattag agagatttaa
     1921 agattctatt aaaatctgtt taaaagaaga agttttatca tttgaaaaag tagatgatgt
     1981 tttcacaatt caaacagata aagatcaaca tttgtcacgt gctattgttt ttgcttgtgg
     2041 caacggtgct tttgctccgc gtttactagg tttggaaaat gaagaaaatt atgcagataa
     2101 taatttattc tataacgtta caaagttaga acagtttgca ggtaaacatg ttgttatttg
     2161 tggtggaggc gactcagctg ttgattgggc caatgaatta gataagattg cagctagtgt
     2221 agcgattgtt catcgtagag atgcttttag agcgcatgag catagtgtgg atatactaaa
     2281 ggcatcaggt gttagaattt taacccctta tgtcccaatt tgtttaaatg gagatagtca
     2341 gagagttagc agcttggtcg ttcaaaaagt taaaggtgat gaagtaattg aattaccttt
     2401 agacaacttg attgttagtt ttggcttttc tacttctaat aaaaatttac gttattggaa
     2461 tttggattat aaacggtcta gtatcaatgt ttcttcactt tttgagacga ctcaagaagg
     2521 cgtgtatgct attggagatg ctgctaatta tccaggaaag gtagaattga ttgcaacagg
     2581 atatggggaa gcaccagtcg ctataaacca agctatcaat tatatttatc ctgatcgaga
     2641 taaccgtgta gtccattcaa catcattgat aaaataacta taaataaaac tgataagttt
     2701 ataaaaaagg attatatata ttgttaaaaa aataacaaag cttcctattt attttgtgat
     2761 ataattaact ttgtcgttat gaatgatatt aaataaatga agcgaggtat tttatgacaa
     2821 atacggtaac accaaaagag acggctggtt cttttatcaa taaagtgtta ggtgggacag
     2881 caactgcaat cgttgtagca ctcatcccaa atgctatcct agccactttt ttgaaaccat
     2941 ttttatctta cggacttgct gcagagttct tacacatcgt gcaggtattc caatttttca
     3001 caccaattat ggcaggattt ttgattggtc aacagtttaa attcacccca atgcaacagc
     3061 ttgctgttgg aggtgctgca tatattggtt ctggtgcgtg ggcttacaca gaagttattc
     3121 aaaaaggagt tgctactggt agtttccaat tgcgtgggat tggtgatctc atcaatatga
     3181 tgctaacagc agctttagca gttttagcag ttaagtggtt tggtaataaa tttggttccc
     3241 taaccattat cttgttacct attatcatag gtacaggcgt aggctattta ggatggaaat
     3301 tactccctta cgtttcatat gtgacaacac ttattggtca aggaattaat tcttttacta
     3361 ctttacaacc aattgcgatg tcaattctaa ttgcgatggc tttttcaatg cttattgtaa
     3421 gtcctatttc aacagtagcg attggtctag cgattggtct taatggtatg tcagcaagtg
     3481 cagcttcaat gggagttgct tcaacaacag cagtgctagt ttgggcaacg atgaaggcta
     3541 ataagtctgg agttccaatt gctattgctt taggtgcaat gaagatgatg atgccaaatt
     3601 tcctaaagca tccagtaatg gcgattccaa tgcttatgac tgctacagta agttctctaa
     3661 cagtcccatt gtttaaattg gtggggacac cagcttcgtc tggttttggg cttgttggtg
     3721 cagtaggtcc gattgcttca tttgaagcag gagcaagtat gttgattgtt atcttatctt
     3781 ggctcgttat tccttttgca gtaggttttg tatcacacaa aatttgtaaa gacattctga
     3841 aactttataa agacgatatt ttcgtttttg aaggtcaaaa ttaatttgga ggttgtatgt
     3901 tagtttatat tgctggttca ggcgctatgg gatgtcgttt tggatatcaa atttctaaaa
     3961 caaatcatga tgttatttta cttgataatt gggcagacca tattatggct attaaagaaa
     4021 atggcttgaa ggtaacaggt gatacagagg accttgttaa acttcctatc atgaagccaa
     4081 ctgatgcgac agaagaagca gatttaatta ttttgtttac taaagcaatg cagttgccaa
     4141 atatgcttca agatattaaa aaaattattg gtaagaaaac aaaggtctta tgtcttttaa
     4201 atggattagg acatgaagat gtcattcgtc aatatatccc tgaacacaat attttgatgg
     4261 gagtaacagt ttggactgca gggttgaaag gaccagggca tgctcatctg gaaggagttg
     4321 gttctgtaaa tcttcaaagt atcgatccta acaatcaaga agcaggtcat cgcgttactg
     4381 aattgcttaa tgaagcaaaa ttacaggcta cttatgacga aaatgttttg ccaaatattt
     4441 ggagaaaagc ttgtgttaac ggaacgatga attcaacttg cgccttgcta gattgtacaa
     4501 ttggtcaact ctttgctagt gaagacggcg taaatatggt acatgaaatt atccatgaat
     4561 ttgttactgt tggaaaagca gaaggtgtag agcttgatga agaagaaata acaaagtacg
     4621 ttatggatac atctgttaaa gcagcccatc attacccttc aatgcatcaa gacttagttc
     4681 aaaatcagcg cttaactgaa attgatttct taaatggcgc agtgaataaa aaaggtgaaa
     4741 acctaggcat tgatacacct tactgtcgat taatcacaca attaattcac actaaagaaa
     4801 atgtacttag cataaaataa taatcaaccg ggagaatagt gcccggtttt ttgatatgct
     4861 taatgataat gattttgtta tactaagaat aataagagtt gggtattttt ttcgagaagt
     4921 atttacagca taaaatatta catttgtatt acaaatattt ctaaaatatg ttgacatgtt
     4981 acaaaaagga tattataata gtagtgtaaa taaataagga agtaggtgtt tatgatgaaa
     5041 aaaggacaag taaatgatac taagcaatct tactctctac gtaaatataa atttggttta
     5101 gcatcagtaa ttttagggtc attcataatg gtcacaagtc ctgtttttgc ggatcaaact
     5161 acatcggttc aagttaataa tcagacaggc actagtgtgg atgctaataa ttcttccaat
     5221 gagacaagtg cgtcaagtgt gattacttcc aataatgata gtgttcaagc gtctgataaa
     5281 gttgtaaata gtcaaaatac ggcaacaaag gacattacta ctcctttagt agagacaaag
     5341 ccaatggtgg aaaaaacatt acctgaacaa gggaattatg tttatagcaa agaaaccgag
     5401 gtgaaaaata caccttcaaa atcagcccca gtagctttct atgcaaagaa aggtgataaa
     5461 gttttctatg accaagtatt taataaagat aatgtgaaat ggatttcata taagtctttt
     5521 tgtggcgtac gtcgatacgc agctattgag tcactagatc catcaggagg ttcagagact
     5581 aaagcaccta ctcctgtaac aaattcagga agcaataatc aagagaaaat agcaacgcaa
     5641 ggaaattata cattttcaca taaagtagaa gtaaaaaatg aagctaaggt agcgagtcca
     5701 actcaattta cattggacaa aggagacaga attttttacg accaaatact aactattgaa
     5761 ggaaatcagt ggttatctta taaatcattc aatggtgttc gtcgttttgt tttgctaggt
     5821 aaagcatctt cagtagaaaa aactgaagat aaagaaaaag tgtctcctca accacaagcc
     5881 cgtattacta aaactggtag actgactatt tctaacgaaa caactacagg ttttgatatt
     5941 ttaattacga atattaaaga tgataacggt atcgctgctg ttaaggtacc ggtttggact
     6001 gaacaaggag ggcaagatga tattaaatgg tatacagctg taactactgg ggatggcaac
     6061 tacaaagtag ctgtatcatt tgctgaccat aagaatgaga agggtcttta taatattcat
     6121 ttatactacc aagaagctag tgggacactt gtaggtgtaa caggaactaa agtgacagta
     6181 gctggaacta attcttctca agaacctatt gaaaatggtt tagcaaagac tggtgtttat
     6241 aatattatcg gaagtactga agtaaaaaat gaagctaaaa tatcaagtca gacccaattt
     6301 actttagaaa aaggtgacaa aataaattat gatcaagtat tgacagcaga tggttaccag
     6361 tggatttctt acaaatctta tagtggtgtt cgtcgctata ttcctgtgaa aaagctaact
     6421 acaagtagtg aaaaagcgaa agatgaggcg actaaaccga ctagttatcc caacttacct
     6481 aaaacaggta cctatacatt tactaaaact gtagatgtga aaagtcaacc taaagtatca
     6541 agtccagtgg aatttaattt tcaaaagggt gaaaaaatac attatgatca agtgttagta
     6601 gtagatggtc atcagtggat ttcatacaag agttattccg gtattcgtcg ctatattgaa
     6661 atttaaagct aaatcttttt agatttagct ttactaatat tatgtgtgtt ggaaaaaaca
     6721 gtggagattt acgtcaaaat tatattattt ggatagtatc gaatagatag aatgtggaga
     6781 aaagtaggaa atagttaaga aaggtattta tgacgcttcg agaattaaca atagaagaat
     6841 ttaaagaaca ttcaggaaat tatgattcac aatcattttt acaaacacct gagatggcta
     6901 aacttttaga aaaacgcggc tatgatgtta ggtatttggg atatcaagta gaaaataaac
     6961 tagagataat cagtttatct tatattatgc cagtcactgg tggttttcaa atgaaaattg
     7021 attcaggacc agttcattca aattctaagt atctaaaaca attttataaa gcattgcaag
     7081 gctatgccaa atccaacggt gttctagaat taatagttga gccttatgat gattaccaat
     7141 tattcactag ttcgggagtt cctagtaatc agggaaatga taatctgatt gaagatttta
     7201 ccagttcagg ttatcaccat gatggtttaa caactggttt tactggtaaa tatttatctt
     7261 ggcactatgt taaaaattta gaaggtgtca cttctgaaac gttactatct tcattctcta
     7321 agacaggacg agctttggtt aagaaagcaa tgtcttttgg aatcaaggtt cgcgttctta
     7381 aacgtgatga gctacattta tttaaagaga taacaacttc tacgtcaaat agacgtgatt
     7441 atatggataa gtccttagat tattatcaag atttttacga tagctttgaa ggcaaggctg
     7501 aatttgtgat tgccacttta aattttagag aatacgacca taacttgcaa ataaaagctg
     7561 aagcattgga aaataagctt aaattgcttg atgaacgttt tagggaaaat gcagactctc
     7621 ctaagtatca taggcaacgc tctgaaataa ttaatcaact ggctagtttt gaaacacgac
     7681 gacaagaagt tcagtcattt attcaaaagt atgataacca agacgttgtt ttagctggta
     7741 gtctatttgt ttatagtctt aaggagacag tttatttctt ttcaggatcc tacacagaat
     7801 ttaataaatt ttatgcacca gcagttcttc aggaatatgt tatgcaagag gctctcaaaa
     7861 gaggttctac tttctataac ctcctaggta ttcagggaac ttttgatggt tcagatagta
     7921 ttcttcgctt taaacagaat tttaatggtt atatcattcg taaaatggga acttttaact
     7981 attatccaag tccttttaaa tacaaaggaa ttcagcttct taaaaaggtt ttgaagagaa
     8041 attaaagtat ttgtaaaagc taatctttag attaggcttt tttagtttat tgatagtttt
     8101 tggaagaaaa tgcttgacat tgaaagaaaa agagatataa ttaaattgca aacgatttca
     8161 attcaaggag ggactatgct aaagtcaaag agaaaagaaa ttatcttatc acgacttgag
     8221 caaaataaga gtgttacttt agatgaatta acgtctatct tagagacttc tgaatctaca
     8281 gttcgcaggg atttagatga acttgaatct gaaggttttt taaaaagggt gcatggtggt
     8341 gctgaattac catactcact tggccaggaa ttaagtaatc aagaaaaagc tatcaaaaac
     8401 gttcaaaaaa aattggatat tgctagacaa actgcaaagc tgatagcaaa gcaagatgtt
     8461 atatttattg atgccggaac tacgacagaa ttattaattg attttctccc tcacgagcaa
     8521 ttaacggtgg tgacgaattc tattcatcat gctgcaaagt tagttgatag agggataaaa
     8581 acaatcatta tcggaggagc ggttaaacat tcaacagatg ctagtattgg ccaggtagcc
     8641 attaatcaga ttaggcagat aacggttgac aaagcttttc tggggatgaa tggtatagat
     8701 gaagtttatt taactacacc agatttagaa gaagctgcta ttaaagaagc tattattaat
     8761 aatagtcaac aaacctttat tctaatggat tcatctaaaa ttggacaagt tacatttgct
     8821 aaagtgaaag agataaatga cattaattta gtaactaata agactgattc agaacttatg
     8881 acaataataa aagagaaaat gaaggtgatt caagtatgat ttatacagta acattaaatc
     8941 catcgattga ttttattgtt cgtctagata cactattgtt gggtagtgtt aaccgtatga
     9001 ctagcgatga taaatatgta ggtggtaaag gaattaatgt tagtcgcata ttaaaacgct
     9061 taaaaattga caatactgct acaggtttta tcggtggatt tacaggacat tttgttgaag
     9121 atggacttgt atcagaaggt atcaaaactg actttgtttc tgttaatgaa gatacacgta
     9181 tcaacgttaa agtaaaagca aaaattgaaa ctgaaatcaa tggcgggggt cctaggataa
     9241 caaatgaaca acttcatcgt ctagagaaac ttctttcgag actaacacct gaggacacag
     9301 tagttttcgc aggttcagca ccagctagcc ttggaaataa ggtctataat acattgattc
     9361 caattgctaa aaagacagga gcggaagttg tttgcgattt tgaaggtcag acacttttag
     9421 acgctcttgc ttatcagcct cttttagtaa aaccgaataa ccatgaatta gcagatattt
     9481 ttggcgtaga gctagaaggt ctgcctgata tcgaaaaata tgcacataag attctagata
     9541 aaggagctaa gaatgttatc gtttctatgg caggggatgg tgcattgtta gtaactccag
     9601 aagcttcata ttttgcaaaa ccgattaagg gtgaagtgaa aaattccgtt ggggctggtg
     9661 attcaatggt agctggcttt acaggagaat ttgttaagtc aaaaaatcca gtagaagcac
     9721 taaaatgggg cgtagcttgt ggtacagcaa ccactttctc agatgattta gcaacagctg
     9781 agtttatcca agatatttat aataaagttg aggtagaaaa attatgaaaa tccaagacct
     9841 actaaaaaaa gaagttatga ttatggattt aaaagcaacc agtaaagagg ctgcaattga
     9901 tgaaatgatt acaaaattag ttgacacagg tgttgtgaca aactttgcta tttttaaaga
     9961 tggtatcatg aagcgtgagg cacaaacatc aactggatta ggtgatggta tcgctatgcc
    10021 acatagtaaa aatgctgccg ttaaagaagc tactgtcctt tttgcaaaat ctgcatcagg
    10081 tgttgattac gaagcacttg atggacagcc aactgattta ttctttatga ttgctgcgcc
    10141 agatggtgct aatgatacgc atttagctgc cttggcagaa ctatctaaat atcttttaaa
    10201 agaagggttc gctgatcaat tacgtcaagc aaaaacgcca gatgacatta ttgctacttt
    10261 tgatagtaat agcatttctc aagaaacagt agctccacaa acagtacaat caacaagtaa
    10321 aggttcagat tatattgttg cagtaacagc ttgcactacg ggaattgccc acacgtatat
    10381 ggcagaagaa gctttaaaga aaaaagcagc tgaaatgggt gtaggtatta aagttgaaac
    10441 aaatggcgct tctggtgtag gaaataagtt gacttcaagt gatattgcaa gggctaaggg
    10501 tgttattatt gcagcagata aggctgtaga gatggatcgt tttgatggca aacctttggt
    10561 atcacgtccg gtagctgatg gtattaaaaa aagtgaagac ttaattaaca ttattctcga
    10621 taataaggca caaacctatc atgcaaaaaa tcaaaatgat aaacaatcag gtgaatctga
    10681 tggaaaatca ggcttaggct ctgcttttta taaacacctt atgggtggtg tatcacaaat
    10741 gttaccattc gttattggtg gtggtattat gattgcgatt gctttccttt ttgataacat
    10801 acttggtgta ccaaaagacc aattaagtaa tttaggttca taccatgaaa ttgcagcctt
    10861 attcaaaaat atcggtggcg cagcatttgc atttatgtta ccagtattgg caggttatat
    10921 cgcttattca atagctgaaa aaccaggact agtagctggc ttcgtagctg gttcaattgc
    10981 atcaagtgga ctggcattcg gtaaagtacc ttttgcagaa ggtgggaaag caaccttagc
    11041 attagcaggt gtgccatcag gtttcttagg ggctttagta ggtggtttct tagctggtgg
    11101 agttatccta ctacttagaa aattactttc aggtctccca aaatcgcttg aaggtattaa
    11161 atcaattctt ctatacccat tgttaggtgt tttgattaca ggtttcttga tgttgctcgt
    11221 aaacattcca atggcagcta tcaacacagc tcttaacact ttcttacaag gtttatctgg
    11281 tagttcagca gtcttgatgg gcttactagt tggtggtatg atggctgtag atatgggtgg
    11341 gcctgttaat aaagcagctt atgtttttgg tacaggtact cttgcagcaa ctgttgcaaa
    11401 tggcggctct gttgttatgg cagctgtaat ggctggagga atggttccac cgttagcagt
    11461 atttgtggca actttattat ttaaagataa atttaataat gaagaacgtc aatctggtct
    11521 aacaaatatc gttatggggt tatcttttat cactgaaggt gctattccgt ttggtgctgc
    11581 tgacccagct cgtgcaatcc ctagctttat tgtaggttca gccttaactg gagcacttgt
    11641 tggtttagct ggtatcaagt tgatggctcc tcatggaggg atttttgtca tcgcattaac
    11701 aagtaatcct ttactttata ttctatttat tctaattgga gcggtagtct caggtgtact
    11761 ctttggcttg ttccgtaaaa caaaatagtt aatctcaact tatccaaatt tacattgaat
    11821 aaaaggtgaa taaactatcg ttattgggtc tattcgcctt tttatgttgt aaaagaatgg
    11881 tagattaatc attaataatt gctaatcttt tttcaatagg atatgattta tttaagcaaa
    11941 aaaatggata atatgattga ggaaaggagg taagcaatga ttaaatggaa tgaaaaagaa
    12001 acaaaactag ttgatacaag tttttcaatt atgttgtatc taaaaacgct atttgcagtc
    12061 ggacaagtag tagctggcgt tttattatat tattttaaac ctacacaaat aaaacatctc
    12121 ctatttcttt taactgaagg tgaagttcac ggtgggattc atggttttat ttctgctcat
    12181 ttcttgaaaa taagcagtta tattaatgat tcggctagtc atttagccgc tatctatcta
    12241 ttaattcatg gcggtatcaa attagtagca cttattttat tacataaaga gaaattattt
    12301 gcttaccctt tatcaatttt agttttcgtt gggtttatca ttttccaact aagggaatat
    12361 ttttctagtc atagcatttt aatgattttt ctatgtgtat ttgatgttat catgataatt
    12421 ttaacatggt tagaatatca gaaaatgaaa ttgaattttt caaaaaggtg acttccccca
    12481 tgccttagcg cttgttttta taagcgcttt tttgctacaa tatgactatg aaaactattg
    12541 aagaaattta caaaaattat cctaatatcc cctatatctc acctgaaaga gacttggctg
    12601 aaattaattt tagtaaagtt gttcctagga aaaatatgga agaaactagt gaaggactct
    12661 tgcctggaga tattatctta ttatggcgta tacagtttgg aactttcaca actgaaacaa
    12721 gtttttcaaa atattttgaa tacatttatg gtataaatgg aaaagaacac ctagaatttt
    12781 taataaaaaa tggatttgta agaatggagt caccgcttga ttctttagat cacttatctg
    12841 ctccactttt aaaactattc ttaaaagaaa aaaacgtaaa aggcttgtca aagatgaaac
    12901 gctctgattt agatcaggca atagctattg cttttacaga agaggaatta gggaagttgt
    12961 ttgttgtcag agggttggca cttacagaaa aaggtttagc agccttgagt aacaatcaag
    13021 aagtgattga tagacatcct aagaagaagt tctagattcc cacctgcttt aagaatctac
    13081 aaatttatgc tataataata catattgtct atcaaggaga aaatacatgg aagtcattcg
    13141 tgaacaagaa tttgtcaatc aataccacta tgatgcgcgc aatttagaat gggaagagga
    13201 aaatggtacc ccaaaaacaa attttgaagt tactttccaa ttagctaata gagatgaagc
    13261 agcaaaagta acgagcattg tagcagtgtt acaatttgtt attgtacgtg atgagtttgt
    13321 tatttctggt gtcatttcac aaatggctca tatccaaggg cgccttatca atgagcctag
    13381 tgagtttagt caagatgaag ttgagaatct agcagctcct cttttagaaa tcgttaaacg
    13441 tctgacttat gaagttacag aaattgcttt agatagacca ggagtaacat tggagtttaa
    13501 tagttaatga aattagcact tattacggat acctcagctt acttgcctga ggctatcgaa
    13561 aatcatgaag atgtctatgt tttagatatt ccaattatca ttgatggtaa aacttatatt
    13621 gagggacaaa atctgacatt agaccaatat tatgataagc tagcggcatc aaaagaatta
    13681 cctaaaacaa gtcagcctag cttagctgaa ctagatgatc tcttatgtca attggagaaa
    13741 gaagggtata cacacgtcct tggtttattc attgcagcgg ggatatcagg tttctggcag
    13801 aatattcagt ttcttattga agaacatcca aatttgacaa ttgcttttcc ggatactaaa
    13861 ataacttcag caccacaggg caatttagtt agaaatgctt tgatgtgttc acgtgaaggg
    13921 atggattttg atgtcatagt taacaagata caatcacaaa ttgaaaaaat tgaaggattt
    13981 attgttgtta atgatttgaa tcatctagta aaaggtggta gattgtcaaa cggttcagct
    14041 atcattggta accttttaag tatcaagccg gtcttacatt ttaatgaaga aggtaaaatc
    14101 gttgtctatg agaaagtgcg tacagagaaa aaagccttaa aacgcttggc tgagattgtc
    14161 aaagaaatga cagcagatgg tgaatatgat attgctatta ttcactctag agcacaagat
    14221 aaggcagaac aattatataa tttgttagct aaagctggtc ttaaagacga tttagaaatt
    14281 gtatcatttg gtggcgttat tgcaactcat cttggtgaag gtgcggttgc ctttggtatt
    14341 accccaaaaa attgatgtgt gataaaaaga gttgcttgga ggttgatcct tattgcagat
    14401 atcaaagaag aagttaggag attctttttt gataatttga gtaggtaggg agtgtccctt
    14461 aggcagttct ttttgtttct gaatccattt ataagaaata ataatgcgac tttagttcgt
    14521 ttagatttgg aatagtatga tgagattaaa ttatttgcct tctgagtttc agaaggcttt
    14581 accaatatta aaaaagataa aaaaagccgg ttatgaagca tactttgtag gtggtagtgt
    14641 tcgggatgtt ttacttgacc gtccaattca tgatgttgat atagcaacgt catcttatcc
    14701 agaagaaaca aagcagattt ttaaaaggac agttgatgtt ggtattgaac atggaacagt
    14761 cttagtattg gaaaaaggtg gtgaatatga gattaccact tttaggactg aggaggttta
    14821 tgttgattat aggcgtccga gtcaggtcaa ttttgttagg tcgctagaag aagatctaaa
    14881 acgtcgagat ttcacagtta atgcctttgc tttaaatgaa gatggagaag ttattgatct
    14941 ctttcatgga ttggatgatt tagataatca ccttcttaga gcagtaggac tcgccagtga
    15001 gcgttttaat gaagatgctc ttcgaattat gcgaggtttg cgtttttctg ctagtctaaa
    15061 ttttgatatc gaaacaacaa cttttgaggc aatgaaaaag cacgcgtcat tattggagaa
    15121 aatatctgtt gagcgttctt ttattgaatt tgataaactt ctattagcac cttattggcg
    15181 taaaggaatg ctggcactaa tagatagtca tgcttttaat tatctaccat gcttaaaaaa
    15241 tagggaatta caattaagcg cctttttgtc ccagttagat aaagattttt tatttgagac
    15301 atcagaacaa gcttgggcat cactcatctt gagtatggaa gttgaacaca caaagacttt
    15361 tttaaaaaaa tggaagacat caactcactt tcaaaaagat gttgagcata tagtggatgt
    15421 ttatcgtatt cgtgaacaaa tgggattgac taaagaacat ctttatcgtt atggaaaaac
    15481 tataataaaa caagcggaag gtattcgcaa agcaagaggc ttgatggttg atttcgaaaa
    15541 aatagaacaa ctagatagtg agttagcaat ccatgatagg catgagatag ttgtcaatgg
    15601 tggcacctta atcaagaaat taggaataaa acctggtcca cagatgggag atattatctc
    15661 tcaaattgaa ttagccattg ttttaggaca actgattaat gaagaagagg ctattttaca
    15721 ttttgttaag cagtacttga tggattagag aggattatat gagcgatttt ttagtagatg
    15781 gattgactaa gtcggttggt gataagacgg tctttagtaa tgtttcattt atcatccata
    15841 gtttagaccg tattgggatt attggtgtca atggaactgg aaagacaaca ctattagatg
    15901 ttatttcggg tgaattaggt tttgatggtg atcgttcccc tttttcatca gctaatgatt
    15961 ataagattgc ttatttaaaa caagaaccag actttgatga ttctcagaca attttggaca
    16021 ccgtactttc ttctgactta agagagatgg ctttaattaa agaatatgaa ttattgctta
    16081 atcactacga agaaagtaag caatcacgtc tagagaaagt aatggcagaa atggattctt
    16141 tagatgcttg gtctattgag agcgaagtca aaacagtatt atccaaatta ggtattactg
    16201 atttgcagtt gtcggttggt gaattatcag gaggattacg aagacgtgtt caattagcgc
    16261 aagtattatt aaatgatgca gatttattgc tcttagacga acctactaac cacttagata
    16321 ttgacactat tgcatggtta acgaattttt tgaaaaatag taaaaagaca gtgcttttta
    16381 taactcatga tcgttatttt ctagacaatg ttgcaacacg tatttttgaa ttagataagg
    16441 cacagattac agaatatcaa ggcaattatc aggattatgt ccgacttcgt gcagaacaag
    16501 acgagcgtga tgctgctagt ttacataaaa agaaacagct ttataaacag gaactagctt
    16561 ggatgcgtac tcagccacaa gctcgtgcaa cgaaacaaca ggctcgtatt aatcgttttc
    16621 aaaatctaaa aaacgattta caccaaacaa gcgatacaag cgatttggaa atgacatttg
    16681 aaacaagtcg aattgggaaa aaggttatta attttgaaaa tgtctctttt tcttacccag
    16741 ataaatctat cttgaaagac tttaatttgt taattcaaaa taaagaccgt attggcatcg
    16801 ttggagataa tggtgttgga aagtcaacct tacttaattt aattgttcaa gatttacagc
    16861 cggattcggg taatgtctct attggtgaaa cgatacgtgt aggttacttt tcacaacaac
    16921 ttcataatat ggatggctca aaacgtgtta ttaattattt gcaagaggtt gcagatgagg
    16981 ttaaaactag tgtcggtaca acaagtgtga cagaactatt ggaacaattt ctctttccac
    17041 gttcgacaca tggaacacaa attgcaaaat tatcaggtgg tgagaaaaaa cgactttacc
    17101 ttttaaaaat cctgattgaa aagcctaacg tgttactact tgatgagccg acaaatgact
    17161 tagatattgc tacattaact gttcttgaaa attttttaca aggctttggt ggtcctgtga
    17221 ttacagttag tcacgatcgt tactttttag ataaagtggc taataaaatt attgcgtttg
    17281 aagataacga tatccgtgaa ttttttggta attatactga ttatttagat gaaaaagcat
    17341 ttaatgagca aaataatgaa gttatcagta aaaaagagag taccaagaca agtcgtgaaa
    17401 agcaaagtcg taaaagaatg tcttactttg aaaaacaaga atgggcgaca attgaagacg
    17461 atattatgat attggaaaat actatcactc gtatagaaaa tgatatgcaa acatgtggta
    17521 gtgattttac aaggttatct gatttacaaa aggaattaga tgcaaaaaat gaagcacttc
    17581 tagaaaagta tgaccgttat gagtacctta gtgagttaga cacatgatta tccgtccgat
    17641 tattaaaaat gatgaccaag cagttgcaca attaattcga caaagtttac gcgcctatga
    17701 tttagataaa cctgatacag catattcaga ccctcactta gatcatttga cctcatacta
    17761 cgaaaaaata gagaagtcag gattctttgt cattgaggag agagatgaga ttattggctg
    17821 tggcggcttt ggtccgctga aaaatctaat tgcagagatg cagaaggtgt acattgcaga
    17881 acgtttccgt ggtaaggggc ttgctactga tttagtgaaa atgattgaag tagaagctcg
    17941 aaaaattggg tatagacaac tttatttaga gacagccagt actttgagta gggcaactgc
    18001 ggtttataag catatgggat attgtgcctt atcgcaacca atagcaaatg atcaaggtca
    18061 tacagctatg gatatttgga tgattaaaga tttataagtt gaaagtggat tagtgaacat
    18121 ggattaatta ttttgagata agaggaaaga aaaggagaca tatatggcat atatttggtc
    18181 ttatttgaaa aggtacccca attggttatg gcttgattta ctaggagcta tgctttttgt
    18241 gacggttatc ctaggaatgc ccacagcctt agcgggtatg attgataatg gcgttacaaa
    18301 aggtgatcgg actggagttt atctgtggac gttcatcatg tttatatttg ttgtactagg
    18361 tattattggg cgtattacga tggcttacgc atctagtcgc ttaacgacaa caatgattag
    18421 agatatgcgt aatgatatgt atgctaagct tcaagaatac tcccatcatg aatatgaaca
    18481 gataggtgta tcttcactag tgacacgtat gacaagcgat acttttgttt tgatgcaatt
    18541 tgctgaaatg tctttacgtt taggcctagt aactcctatg gtaatgattt ttagcgtggt
    18601 tatgatacta attacgagtc catctttggc ttggcttgta gcggttgcga tgcctctttt
    18661 ggtaggagtc gttttatatg tagctataaa aacaaaacct ttatctgaaa gacaacagac
    18721 tatgcttgat aaaatcaatc aatatgttcg tgaaaattta acagggttac gcgttgttag
    18781 agcctttgca agagagaatt ttcaatcaca aaaatttcaa gtcgctaacc aacgttacac
    18841 agatacttca actggtcttt ttaaattaac agggctaaca gaaccacttt tcgttcaaat
    18901 tattattgca atgattgtgg ctatcgtttg gtttgctttg gatcccttac aaagaggtgc
    18961 tattaaaata ggggatttag ttgcttttat cgaatatagc ttccatgctc tcttttcatt
    19021 tttgctattt gccaatcttt ttactatgta tcctcgtatg gtggtatcaa gccatcgtat
    19081 tagagaggtg atggatatgc caatctctat caatcctaat gccgaaggtg ttacggatac
    19141 gaaacttaaa gggcatttag aatttgataa tgtaacattc gcttatccag gagaaacaga
    19201 gagtcccgtt ttgcatgata tttcttttaa agctaagcct ggagaaacaa ttgcttttat
    19261 tggttcaaca ggttcaggaa aatcttctct tgttaatttg attccacgtt tttatgatgt
    19321 gacacttgga aaaatcttag tagatggagt tgatgtaaga gattataacc ttaaatcact
    19381 tcgccaaaag attggattta tcccccaaaa agctctttta tttacaggga caataggaga
    19441 gaatttaaaa tatggaaaag ctgatgctac tattgatgat cttagacaag cggttgatat
    19501 ttctcaagct aaagagttta ttgagagtca ccaagaagcc tttgaaacgc atttagctga
    19561 aggtgggagc aatctttctg ggggtcaaaa acaacggtta tctattgcta gggctgttgt
    19621 taaagatcca gatttatata tttttgatga ttcattttct gctctcgatt ataagacaga
    19681 cgctacttta agagcgcgtc taaaagaagt aaccggtgat tctacagttt tgatagttgc
    19741 tcaaagggtg ggtacgatta tggatgctga tcagattatt gtccttgatg aaggcgaaat
    19801 tgtcggtcgt ggtacccacg ctcaattaat agaaaataat gctatttatc gtgaaatcgc
    19861 tgagtcacaa ctgaagaacc aaaacttatc agaaggagag tgattgtatg agaaaaaaat
    19921 ctgttttttt gagattatgg tcttacctaa ctcgctacaa agctactctt ttcttagcga
    19981 tttttttgaa agttttatct agttttatga gcgttctgga gccttttatt ttagggttag
    20041 cgataacaga gttgactgct aaccttgttg atatggctaa gggagtttct ggggcagaat
    20101 tgaacgttcc ttatattgct ggtattttga ttatttattt tttcagaggt gttttctatg
    20161 aattaggttc ttatggctca aattatttta tgactac
//
LOCUS       DATRNK010000036        20129 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.036, whole genome shotgun sequence.
ACCESSION   DATRNK010000036 DATRNK010000000
VERSION     DATRNK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20129
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.036"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            48..698
                     /locus_tag="VBC52_001265"
     CDS             48..698
                     /locus_tag="VBC52_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6882852.1"
                     /translation="MYKEGDLIQLNQRQERIIDIIKQFQPITGELIAEKLGVTRAALR
                     SDLAVLSMLGIIDAKPKVGYFYLGQYHASIGTSHFEKMTVSEIMGIPLTVHQKDSVYD
                     VIVHIFMEDAGCAFILDDDDFLCGVVSRKDLLKTSIGGGDLSKMPIGMVMTRMPHVTT
                     VLENESLFAAADKLVSRKVDSLPVVRHDKQYPEKFKVIGKLSKTILASLFLEIRDY"
     gene            709..1539
                     /locus_tag="VBC52_001266"
     CDS             709..1539
                     /locus_tag="VBC52_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEO6882853.1"
                     /translation="MEDQLTIFIISDSLGETAKAIAKACLSQFPGHDDWHFQRFSYIN
                     SQERLEQVFEEASQKTVFMMFSLVDVALASYAQKRCESEHYAYVDLLTNVIQGISRIS
                     GIDPLGEPGILRRLDNDYFKRVESIEFAVKYDDGRDPRGILQADLVIIGISRTSKTPL
                     SMFLADKNIKVINIPLVPEVPVPKELRMIDSRRIIGLTNSVDHLNQVRKVRLKSLGLS
                     STANYASLERILEETRYAEEVMKNLGCPIINVSDKAIEETATIILEILKTNGQVAKNL
                     "
     gene            1552..4197
                     /locus_tag="VBC52_001267"
     CDS             1552..4197
                     /locus_tag="VBC52_001267"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEO6882854.1"
                     /translation="METKFVYHFDEGCKEMKELLGGKGANLAEMTSIGLPVPQGFTIT
                     TQACNDYYDNACHIRESILSQIDQAMAQLEVEQNKQLGSVDDPLLVSVRSGSVFSMPG
                     MMDTVLNLGLNDRSVQGLVKKTEDERFAYDSYRRFIQMFADVVTGIPKYKFDTILDRL
                     KTDKCYQDDTELTGSDLKRLVEFYKELYQKEAGEKFPQDPKRQLLLAIEAVFKSWNNP
                     RAKIYRKLNDIPETLGTAVNIQAMVFGNMGNNSGTGVAFTRNPSTGAANLFGEYLINA
                     QGEDVVAGIRTPQSISKLAEQMPIIYQEFVSVTQKLEAHYRDMQDMEFTIENGNLYML
                     QTRSGKRTAKAAIKIAVDQVNEGLISKEEAILRIEPKQLDQLLHPSFDLKSLKKAIIL
                     TTGLPASPGAAYGKVYFHAEDVVKEMKKGNPVLLVRQETSPEDIEGMVSANGIITARG
                     GMTSHAAVVARGMGKPCVAGCSQLLVDEVRREISIGHQTIKEGEMLSIDGATGNVYIG
                     QVPMAETSVDRDFEIFMKWVDENRDMMVCSNADNPRDAQKALDFGAEGIGLCRTEHMF
                     FDDERIPVVREMILADEILSRRKALERLLSFQRDDFYQIFKVLKGKACTIRLLDPPLH
                     EFLPHDKESIESMARQMGISTLAIEKRIQTLEEFNPMLGHRGCRLAITYPEIYQMQVR
                     ALVQGAILAMKEGYEAKPEIMIPLVTAHEEISIIRDLIEETIVEESKSKKINLSFPIG
                     TMIETPRACMIADDIAKFADFFSFGTNDLTQMSFGFSRDDAGKFLGEYVDKGLLKKDP
                     FQVLDQKGIGRFIEQAVRLGKEVKPNLKIGICGEHGGEPSSIEFCYQLGLHYVSCSPF
                     RIPIAKLAAAQAKIKQSRNDITIDK"
     gene            4335..4637
                     /gene="gatC"
                     /locus_tag="VBC52_001268"
     CDS             4335..4637
                     /gene="gatC"
                     /locus_tag="VBC52_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEO6882855.1"
                     /translation="MKISEEEVRHVANLSKLRFSDQETKEFASSLSKIVDMIELLNEV
                     DTEGVPVTTTMADRKTVMREDIAQPGHNRDDLFKNVPQHQDYYIKVPAILEDGGDA"
     gene            4637..6103
                     /gene="gatA"
                     /locus_tag="VBC52_001269"
     CDS             4637..6103
                     /gene="gatA"
                     /locus_tag="VBC52_001269"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEO6882856.1"
                     /translation="MSFNNQSIDQLHDFLVKKEISATELTKATLEDIHAREQAVGSFI
                     TISDEMAIAQAKEIDDKGIDADNVMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATAVEKLYAKDMIVIGKANMDEFAMGGSTETSYFKKTNNAWDHSKVPGGSSGGSAAAV
                     ASGQVRLSLGSDTGGSIRQPASFNGIVGMKPTYGRVSRFGLFAFGSSLDQIGPMSQTV
                     KENAQLLTVISGHDVRDSTSSERTVGDFTAKIGQDIQGMKIALPKEYLGEGIAQGVKE
                     TIIKAAKHLEKLGAVIEEVSLPHSKYGVAVYYIVASSEASSNLQRFDGIRYGYRTENY
                     KNLDDIYVNTRSEGFGDEVKRRIMLGTFSLSSGYYDAYYKKAGQVRSLIIQDFEKVFA
                     DYDLILGPTAPTTAFDLDSLNHDPVAMYLADILTIPVNLAGLPGISIPAGFDQGLPVG
                     MQLIGPKFSEETIYQVAAAFEATTDYHKQQPKIFGGEN"
     gene            6103..7545
                     /gene="gatB"
                     /locus_tag="VBC52_001270"
     CDS             6103..7545
                     /gene="gatB"
                     /locus_tag="VBC52_001270"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEO6882857.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPSSAHFGQEQNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHQNMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIELEDGTRKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEEFGTKAELKNLNS
                     FNNVRKGLIHEEKRQAQVLRSGGQIQQETRRFDETTGETILMRVKEGSSDYRYFPEPD
                     LPLFDISDEWIDQVRLELPEFPQERRAKYVSSFGLSSYDASQLTATKATSDFFEKAVA
                     IGGDAKQVSNWLQGEVAQFLNSESKSIEEIGLTPENLVEMIGLIADGTISSKIAKKVF
                     VHLAKNGGSAEEFVKKAGLVQISDPEVLIPIIHQVFADNEAAVIDFKSGKRNADKAFT
                     GYLMKATKGQANPQVALKLLAQELAKLKEE"
     gene            7675..8589
                     /locus_tag="VBC52_001271"
     CDS             7675..8589
                     /locus_tag="VBC52_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEO6882858.1"
                     /translation="MTKKEKGTMMTLAAGLAWGISGISGQYLMSHGVHVNLLTSLRLL
                     ITGIFLLSLARSKQKEHLVAAWKQPKFLKQVLLFSIFGLVLNQYAFLRAIHLTNAGTA
                     TVLQYMAPILILSIVCILNRQRPTSFEIIAIAMAILGTYMIATHGKLGSLAITPKGLM
                     WGLGSAITYSIYILLPVKLIHEWGSTIVIGSGMFIGGILFSLVTKAWQYPLQINVMSI
                     LAYIGIIGIGTIFAYTFFLKGVSIVGAVKGSLLASVEPVSSVFLTVLVLGEIFYPIDL
                     LGMLFIFLAVTLISYKDLIALKKMKRVR"
     gene            8673..9233
                     /locus_tag="VBC52_001272"
     CDS             8673..9233
                     /locus_tag="VBC52_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEO6882859.1"
                     /translation="MNSKIYFLEMKLSIDDYRPTFVVEAVYDLRAEDLLRHGIRAVLV
                     DLDNTLIAWNNPDGTAEVRAWLDEMTTADISVVVVSNNNHARVERAVSRFGVDFVSRA
                     MKPFTRGINMAIERYGFDRDEVIMVGDQLMTDIRASHRAGIKSVLVKPIVKSDAWNTK
                     FNRLRERRVWKKIEENYGKIVYQKGI"
     gene            9233..10351
                     /gene="yqeH"
                     /locus_tag="VBC52_001273"
     CDS             9233..10351
                     /gene="yqeH"
                     /locus_tag="VBC52_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEO6882860.1"
                     /translation="MEELFCIGCGARIQTENKDAAGYTPRAALEKGLETGELYCQRCF
                     RLRHYNEITDVHITDDEFLKLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFVAGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPVDVILTSAQNHHAIKDLIDTIEKY
                     RHGRDVYVVGVTNVGKSTLINAIIREITGSRDVITTSRFPGTTLDKIEIPLDDGSYIF
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNSEQTLFLAGLARFDFISGQKQ
                     GFTAYFDNNLNLHRTKLVGADEFYTKHVGKLLTPPTGKEVSDFPKLVRHEFTIKDKMD
                     IVYSGLGWIRVKSEAENPVVVAVWAPEGVAVVLRKALI"
     gene            10444..10761
                     /gene="yhbY"
                     /locus_tag="VBC52_001274"
     CDS             10444..10761
                     /gene="yhbY"
                     /locus_tag="VBC52_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEO6882861.1"
                     /translation="MLTSKQRAFLKSEAHSMKPIIQIGKNGLNDQIKTSVRNALDARE
                     LIKVTLLQNTDEDIHDVAEVLEDEIGCDTVLKIGRILILYKESARKENRKISVKVKAV
                     KSN"
     gene            10891..11523
                     /locus_tag="VBC52_001275"
     CDS             10891..11523
                     /locus_tag="VBC52_001275"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEO6882862.1"
                     /translation="MALELLTPFTKVELEEKKRDTNRKQIGIMGGNFNPVHNAHLVVA
                     DQVRQQLCLDQVLLMPEFQPPHIDKKETIDEQHRLKMLELAIEGIDGLSIEPIEIERK
                     GISYTYDTMKLLIEKNPDVDYYFIIGADMVEYLPKWHRIDELVKMVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRQFIKSNRQPNYLLPREVLDYIRKEGLYK"
     gene            11520..12107
                     /gene="yqeK"
                     /locus_tag="VBC52_001276"
     CDS             11520..12107
                     /gene="yqeK"
                     /locus_tag="VBC52_001276"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEO6882863.1"
                     /translation="MTYKDYTGLDRTELLSKVRHMMSDKRFNHVLGVERAAIELAERY
                     GYDKEKAGLAALLHDYAKELSDDEFLRLIDKYQPDPDLKKWGNNIWHGLVGIYKIQED
                     LAIKDQDILAAIAKHTVGSAQMSTLDKIVYVADYIEHNRDFPGVEEARELAKVDLNKA
                     VAYETARTVAFLASKAQPIYPKTIETYNAYIPYLD"
     gene            12170..12691
                     /locus_tag="VBC52_001277"
     CDS             12170..12691
                     /locus_tag="VBC52_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO6882864.1"
                     /translation="MTCALLLIDIQQGIMDKKPKHLTNFAVLLDDLLLSAKGSNCEVI
                     WIRHHDKELPQGSPQWEIWEQRHLVTHHKIIDKTYNSCFKDTHLHDYLQSKHISQLIM
                     MGLQTEYCFDTSVKVAFEYGYDIFIPQGGHLTFDTPTLSGDSIKKHYENIWHHRFATM
                     VAKDSLLKLINEE"
     gene            12693..13049
                     /gene="rsfS"
                     /locus_tag="VBC52_001278"
     CDS             12693..13049
                     /gene="rsfS"
                     /locus_tag="VBC52_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEO6882865.1"
                     /translation="MTEKDLLQLVVKAADEKRAEDIVILDLQPVTSVADYFVIMSASN
                     SRQLEAIADNIREQVKGNGGDASHLEGDSKAGWVLLDLNSVVVHIFSEDERQHYNLEK
                     LWHEAPLLDAEVFMTE"
     gene            13115..13849
                     /locus_tag="VBC52_001279"
     CDS             13115..13849
                     /locus_tag="VBC52_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6882866.1"
                     /translation="MATYETFAAVYDAVMDDTLYAKWTDFSLRHFPKGKKKLLELACG
                     TGIQSVRFAQAGYAVTGLDLSGDMLKLAKKRATSAHQSIQFIEGNMLDLSNVGKYDLI
                     TCYSDSICYMQDEVEVGDVFIEVYKALEENGVFIFDVHSTYQTDKVFPGYSYHENADD
                     FAMVWDTYEDDAPHSIVHELTFFVQEEDGRFTRHDEVHEERTYDILTYDILLEQAGFK
                     DVKVYADFEDKKPTATSARWFFVAHK"
     gene            13872..14123
                     /locus_tag="VBC52_001280"
     CDS             13872..14123
                     /locus_tag="VBC52_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882867.1"
                     /translation="MIIFLYACLMIFGILYIIERRVSQKKSKVILELLMNPKLHLDLL
                     SALKPFEKETDRLIYLRKTYHLDIIDAVTLNNQLKEKEV"
     gene            14120..15229
                     /locus_tag="VBC52_001281"
     CDS             14120..15229
                     /locus_tag="VBC52_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEO6882868.1"
                     /translation="MTVTGIVAEFNPFHNGHKYLLEQAQGIKVIAMSGNFMQRGEPAI
                     VDKWTRSQMALENGADLVIELPFLVSVQSADYFASGAVSILARLGVDNLCFGTEEMLD
                     YARIGDIYVNKKEEMEAFLKKQSDSLSYPQKMQAMWQEFAGITFSGQTPNHILGLAYT
                     KAASQNGIRLNPIQRQGAGYHSSEKTEIFASATSLRKHQSDRFFVEKGMPNSDLFLNS
                     PQVVWQDYFSLLKYQIMTHSDLTQIYQVNEEIANRIKSQIRYVETVDELVDKVATKRY
                     TKARIRRLLTYILINAVESPIPNAIHVLGFTQKGQQHLKSVKKSVDIVTRIGSQTWDS
                     LTQRADSVYQMGNANIAEQTWGRIPFYQPVSQSDL"
     gene            15322..16035
                     /locus_tag="VBC52_001282"
     CDS             15322..16035
                     /locus_tag="VBC52_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6882869.1"
                     /translation="MKTVKEISHISGISVRTLHYYDEIDLLSPSFVGENGYRYYDDES
                     LIKLQEILLFKELEFPLKKIKEIMDSPNYDRNQALLDQIRWLELKKQRLEEVIEHAKS
                     IQRGKNMSDFTAYNQEELEAFQEEARTRWGDTDSYKEFENSHSKNDFSMISQAMSQIF
                     KDFGQLKELSPTDEKFQKQVQILQDYITAQFYNCTNDLLASLGIMYIQDERFQKSIDN
                     WGGQGTALFVSKAIDSYCH"
     gene            16174..16578
                     /locus_tag="VBC52_001283"
     CDS             16174..16578
                     /locus_tag="VBC52_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO6882870.1"
                     /translation="MKGFHGNIEKLTLGNTNFRQVLYTAEHCQLVLMTLPVGGEIGSE
                     IHAENDQFFRFEAGHGKVVIDGNEYEVADGDAIIVPAGAEHNVINTSETEMLKLYTIY
                     SPAHHKDGIIRATREEAEENEEDFDGVTTETK"
     gene            complement(16843..17556)
                     /locus_tag="VBC52_001284"
     CDS             complement(16843..17556)
                     /locus_tag="VBC52_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6882871.1"
                     /translation="MTQFLGEFLGTFILVLLGDGVVAGNVLSKTKEEGTGWTAIVFGW
                     GIACTVAVYVSGLFSPAHLNPAVTLAMASIGAISWGQVIPFIIAQMLGAMVAATILWL
                     HYYPHWKETKDSGLILASFSTGPAIRHTPSNLLGEIIGTAILVITIMAIGPSKVAAGL
                     GPIIVGIVIFAVGFSLGPTTGYAINPARDLGPRLMHAILPIENKGNSDWSYAWIPVVG
                     PIIGGVLGAILYNIALTLV"
     gene            complement(17566..17940)
                     /locus_tag="VBC52_001285"
     CDS             complement(17566..17940)
                     /locus_tag="VBC52_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEO6882872.1"
                     /translation="MSDIGIIVVSHSKNIAQGVVDLISEVAKDVSITYVGGTEDGEIG
                     TSFDQVQQIVEQNDKKTLLAFFDLGSAKMNLELVADFSEKNIIINSVPVVEGAYTAAA
                     LLQAGADLDSIQSQLAELTINK"
     gene            complement(17940..18518)
                     /gene="dhaL"
                     /locus_tag="VBC52_001286"
     CDS             complement(17940..18518)
                     /gene="dhaL"
                     /locus_tag="VBC52_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEO6882873.1"
                     /translation="MEVKTAIEWMHTFNQKIQSNKDYLSELDTPIGDGDHGGNMARGM
                     TAVIENLDNNEFSSAADVFKTVSMQLLSKVGGASGPLYGSAFMGITKAEQSKSTISEA
                     LGAGLEMIQKRGKAELNEKTMVDVWHGVIEAIEKNELTEDRIDSLVDATKGMKATKGR
                     ASYVGERSVGHIDPGSFSSGLLFKALLEVGGV"
     gene            complement(18556..19545)
                     /gene="dhaK"
                     /locus_tag="VBC52_001287"
     CDS             complement(18556..19545)
                     /gene="dhaK"
                     /locus_tag="VBC52_001287"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEO6882874.1"
                     /translation="MKKILNQPTDVVNEMLDGLAYVHNDLVHRIEGFDIIARNEEKSG
                     KVALISGGGSGHEPSHAGFVGEGMLSAAVCGAVFTSPTPDQVLEAIKEADEGAGVFMV
                     IKNYSGDIMNFEMAQDMAEMEGIEVASVVVDDDIAVEDSLYTQGKRGVAGTILVHKIL
                     GHAARHGKSLQEIKAIADELVPNIHTVGLALSGATVPEVGKPGFVLAEDEIEFGIGIH
                     GEPGYRKEKMQPSKALATELVDKLIESFDAKSGEKYGVLINGMGATPLMEQYVFANDV
                     AKLLEDKGIEVNYKKLGNYMTSIDMAGLSLTLIKLENQEWLEALNSDVTTIAW"
     gene            19719..>20129
                     /gene="dhaS"
                     /locus_tag="VBC52_001288"
     CDS             19719..>20129
                     /gene="dhaS"
                     /locus_tag="VBC52_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEO6882875.1"
                     /translation="MTSSLITKKKIAKSFKRLFISQAFDKISVSDIMEDAGIRRQTFY
                     NHFVDKYALLEWIFQTELSEQVTDNLDYISGFQLLSELLTFFKMNQEFYIKLFQIEDQ
                     NDFSSYFESYCEQLVDKLLSDYSKSNFNQKERVTF"
BASE COUNT         6641 a         3199 c         4043 g         6246 t
ORIGIN      
        1 cagtaaaaaa agatatataa tataacatat aaaataaata gtatacaata tataaagaag
       61 gtgatcttat tcaattaaat caaaggcaag aaagaattat tgatattatc aaacagtttc
      121 agcccattac tggtgagcta atagctgaga aattaggagt aacaagagca gcactaaggt
      181 ctgatttggc ggttttaagt atgctaggta tcatagatgc aaaacctaag gttggttatt
      241 tttatttagg acagtatcat gcttcaatag ggacaagtca ttttgaaaag atgacagttt
      301 cagaaattat ggggatccct ctgacagttc atcaaaaaga ttcagtttat gatgttattg
      361 tacatatttt tatggaagat gctggttgtg cttttatctt ggatgatgat gattttctct
      421 gtggagtcgt gtcacgtaaa gatttactaa aaaccagtat tggcggagga gatctttcta
      481 aaatgccaat aggaatggtg atgacacgta tgccacacgt gacaactgtt ttagaaaatg
      541 aaagtctttt tgcggcagct gataaattag tgagcagaaa agtggatagt ctccctgtcg
      601 ttcgtcatga taagcaatat cccgaaaaat ttaaagtgat cgggaaatta tctaaaacaa
      661 tcctcgctag tttgttttta gaaataagag attattagga ggaaactgat ggaagatcaa
      721 ctcactatat ttattatttc agattcttta ggggaaacag ccaaagctat tgcgaaagca
      781 tgtttgtcac aatttccagg acatgatgat tggcattttc aacgtttttc ctatattaac
      841 agtcaggaac gcttagaaca agtttttgaa gaggctagcc agaagacagt atttatgatg
      901 tttagtttag ttgatgttgc tttggcttcc tatgcacaga aacgatgtga aagtgaacat
      961 tatgcttatg ttgacttatt aacaaatgtt attcaaggaa tatcaaggat atcgggtata
     1021 gatcctttgg gagaaccagg gattttacga cgcttagaca atgattattt caaaagagta
     1081 gaatcaatag aatttgcagt aaaatatgat gatgggagag atccgcgagg tattttacaa
     1141 gcagatttag ttattatagg aatttcaaga acttctaaaa caccattaag tatgttttta
     1201 gcagataaaa atattaaagt tattaatatt cctttggttc cagaggttcc tgttcctaaa
     1261 gaattacgta tgattgattc tagaaggatt atcggactaa ctaattcggt tgatcatctt
     1321 aatcaagtcc gaaaagttcg attaaagtcc ttaggtcttt ctagtacggc aaattatgcc
     1381 agtttagaac ggattttgga ggagacgagg tatgctgaag aagttatgaa aaatttaggt
     1441 tgcccaatca ttaatgtatc tgataaagca atcgaagaaa cagcaacgat tattttagaa
     1501 atcttaaaaa caaatggaca agtagctaaa aatctgtaaa aggagacgct gatggaaact
     1561 aagtttgtgt atcattttga tgaaggttgt aaggaaatga aggaactgct aggtggtaag
     1621 ggggctaatc tagctgaaat gacgagtatt gggttaccgg taccacaagg ttttactatt
     1681 acaacacagg cttgtaatga ttattatgat aacgcttgcc atatccgaga gagtattctt
     1741 agtcaaattg atcaagcaat ggcacaattg gaggttgaac agaacaagca attaggaagt
     1801 gttgatgatc ctttgttggt gtcagttcgg tcaggatcag ttttttcaat gcctggtatg
     1861 atggacacag ttttaaattt agggttaaat gatcgtagtg tccaaggttt agtgaaaaag
     1921 acagaagacg agcgttttgc ttatgacagt tatcgtcgtt ttatccaaat gtttgcagat
     1981 gtggttacag gcattccaaa atataaattt gataccattt tagatcgact taagacagat
     2041 aaatgttatc aagatgacac agaactaact ggttcagatt taaagagact tgttgaattt
     2101 tacaaggaac tatatcaaaa agaagcagga gaaaaatttc cgcaagatcc taaaaggcag
     2161 cttttgttag caattgaggc agtttttaaa tcatggaata atccaagagc taagatttat
     2221 cgtaaattaa atgatattcc ggagacgctt gggacagccg ttaatatcca agctatggtt
     2281 tttgggaata tgggaaataa cagcggaacg ggagttgctt ttacacgaaa cccatcaact
     2341 ggagcagcaa atttatttgg tgagtatttg attaatgcac aaggagaaga tgttgttgca
     2401 ggtatcagaa caccacaatc catatcaaaa ttggcagaac aaatgcctat tatttaccaa
     2461 gagtttgtaa gcgttaccca aaagttagaa gctcattatc gcgatatgca agatatggag
     2521 tttacaattg aaaatggtaa tctttatatg cttcagacga gaagcggtaa gcgtacagcg
     2581 aaggcagcta tcaaaatagc agtagatcaa gtaaatgaag gcctaattag taaagaagaa
     2641 gctatactac gtatcgagcc caaacaatta gatcagttat tacacccttc ttttgacttg
     2701 aaaagtttaa agaaagctat tattttgacg acaggacttc cagcttctcc tggagctgct
     2761 tatgggaaag tttatttcca tgcagaagat gttgttaaag agatgaaaaa gggaaatcca
     2821 gtgctattgg tcagacaaga gacatcacct gaagatatcg aggggatggt tagtgcaaac
     2881 gggattataa cagcaagagg cggaatgact tcccatgcgg cagtagtagc cagaggaatg
     2941 gggaaaccat gcgttgcagg ctgtagtcaa ttattggttg atgaagtaag acgagaaatt
     3001 tctattgggc atcaaacgat aaaagaaggt gagatgttat caatcgatgg tgctacagga
     3061 aatgtttata taggacaagt tccgatggca gagactagcg tagatcgtga ttttgagata
     3121 tttatgaaat gggtagatga aaatcgagat atgatggtgt gttcaaatgc agataatccg
     3181 agagatgctc aaaaggcctt ggactttgga gcagaaggaa taggtctttg tcgcacggaa
     3241 catatgtttt ttgacgatga acgtattcca gtcgttagag aaatgatttt agcggatgaa
     3301 atattgagcc gaaggaaggc tctagaaagg cttttatctt tccaaagaga cgatttttat
     3361 caaattttta aagtattgaa aggtaaagct tgtacgattc gtttgcttga ccctccttta
     3421 catgaattct taccccatga taaagagtct atagaatcta tggcaagaca aatggggata
     3481 tctactttag ctattgaaaa acggattcaa acattagaag aattcaaccc tatgttaggt
     3541 catcgtggtt gtagacttgc gattacctat ccggaaattt accaaatgca ggtgagagct
     3601 cttgtacaag gcgctatact tgcaatgaaa gagggatatg aggcaaagcc agaaattatg
     3661 attcctttag tgacagcgca tgaagaaatc tctataatac gtgatctgat tgaagagaca
     3721 attgttgagg agagtaaatc taaaaaaatt aacttatcat tccctattgg gacaatgatt
     3781 gaaacaccaa gagcttgtat gatagcggat gatattgcaa agtttgcgga tttctttagt
     3841 tttgggacca atgatttaac acaaatgagc tttggatttt cacgagatga tgctggaaaa
     3901 ttcttaggag aatacgttga taaaggactt ctaaaaaaag accctttcca agttttagat
     3961 caaaaaggaa taggaaggtt tattgagcag gcagtgaggt taggaaaaga agttaagcca
     4021 aaccttaaaa taggtatctg tggagagcat ggtggagaac catcatctat tgagttctgt
     4081 taccaactgg ggttacatta tgtatcgtgt tcgccgttta ggattcctat tgctaaatta
     4141 gcggctgcac aggctaaaat caaacaaagc agaaatgata taacaataga taaataaaca
     4201 gtaaacgctt cttatcatag gtaagaagcg tttattatga aaagctggga taatgtgata
     4261 acattgagag tttaatatgc tataatagta gagactatct taaaacgaaa atgatacaga
     4321 gataaggaga actcatgaaa atttctgaag aagaagtgcg tcatgttgcc aatctgtcaa
     4381 aattgcgctt ttctgatcaa gagactaaag aatttgcgtc aagtttgtca aagattgttg
     4441 atatgattga actcttaaat gaagttgaca cagaaggcgt accagtaaca acgaccatgg
     4501 cagatcgcaa aaccgtgatg cgtgaagata ttgctcaacc aggccataat cgtgatgact
     4561 tatttaaaaa tgtaccacag catcaagatt attatattaa agtacctgca attcttgagg
     4621 atggaggaga tgcctaatgt cgtttaataa tcaatcaatt gatcagttgc atgattttct
     4681 cgttaaaaaa gaaatttcag caactgaatt aacaaaagct acattggaag atattcatgc
     4741 gcgtgaacaa gcggttggat cttttattac tatttctgat gagatggcaa tagctcaagc
     4801 caaagaaatt gatgataaag gaattgatgc tgataatgtt atgtcaggta ttcccttagc
     4861 agttaaagat aatatttcaa caaaggggat tcttacaaca gctgcttcta aaatgctcta
     4921 taactatgag ccaatctttg atgctacggc tgttgaaaag ttatatgcta aagatatgat
     4981 tgtgattggt aaagcaaaca tggatgaatt tgccatggga ggctctacag aaacatctta
     5041 ttttaaaaag acaaataacg cttgggacca tagtaaagtt ccgggaggat catcaggtgg
     5101 ttctgcagca gcagtagctt caggacaagt acgtttatca ttgggctctg atacaggtgg
     5161 ttcaattcgc caaccggctt catttaatgg aatcgttggt atgaagccaa cttacggacg
     5221 tgtatcacgc tttggtttgt ttgctttcgg tagttcgctt gaccagattg gaccgatgtc
     5281 tcaaacagta aaagagaatg cacagctttt gacagttatt tctgggcatg atgtaagaga
     5341 ttcaacttct tctgagcgta ctgtaggcga ttttactgct aaaattggtc aagatattca
     5401 aggaatgaaa attgctttac caaaagaata tttgggagaa ggaatagcgc aaggagttaa
     5461 agaaacgatt attaaggccg caaaacatct ggaaaaacta ggtgcagtta ttgaggaagt
     5521 tagtttaccg cattctaaat acggtgttgc tgtttattat attgttgctt catcggaagc
     5581 ttcttcaaac ttacaacgtt ttgatggtat tcgttatgga taccgtacag aaaattataa
     5641 aaacttggat gatatctatg tcaatacacg atctgaaggt tttggagacg aagttaagcg
     5701 tcgcattatg ctgggaacgt tcagtttatc ttctgggtat tacgatgctt attataaaaa
     5761 agcaggtcaa gttcgtagtt tgattattca agattttgaa aaagttttcg cagattatga
     5821 cttgatttta ggtccaacag ctccaacaac agcctttgac ttagattctt taaatcacga
     5881 tccagttgca atgtacctag ctgatattct aacaatacca gttaatttgg ctggtttacc
     5941 aggtatttca atccctgctg gttttgatca aggtctaccg gttggtatgc agttgattgg
     6001 tccgaaattc agtgaagaaa caatatatca ggttgctgct gccttcgaag caacgacaga
     6061 ttatcataaa caacaaccta aaatttttgg aggtgagaac taatgaattt tgaaacagtc
     6121 attggacttg aagtccatgt tgaattaaat acgaactcta aaattttctc accttcttcc
     6181 gctcattttg gtcaggaaca aaatgctaat acaaatgtaa ttgactggtc tttcccaggt
     6241 gtccttccag tcatgaataa aggagttatt gatgcaggaa tcaaggcggc acttgccttg
     6301 aatatggata tccatcaaaa catgcatttt gatcgtaaaa attatttcta tccagataat
     6361 cctaaagctt atcaaatttc acagtttgat gagcctattg gttacaatgg ttggattgaa
     6421 atcgaattag aagatggtac acgaaaaaaa attcgtattg agcgtgccca cctcgaagaa
     6481 gatgcaggaa aaaatacaca tgggacagat ggttattcct atgttgacct taatagacaa
     6541 ggtgttccac tgattgaaat tgtatcagaa gctgatatgc gttctcctga ggaagcttat
     6601 gcttatttga cagctctcaa agagattatc caatatactg gaatttctga cgttaaaatg
     6661 gaagaaggtt ctatgcgtgt agatgccaat atttcacttc gtccttatgg tcaagaagag
     6721 tttggtacta aagcagaact taagaacctt aactcattca ataatgtacg taaaggtttg
     6781 atacatgaag aaaaacgtca agcacaagtt ttacgatctg gtggtcaaat tcagcaagag
     6841 actcgtcgtt ttgatgaaac aacaggtgag actattttga tgcgtgtcaa ggaaggctca
     6901 tcagattatc gctatttccc tgaaccagat ctaccactat ttgatatctc ggatgaatgg
     6961 attgatcaag ttcgtttaga attaccagaa tttccacagg aacgtcgtgc gaaatatgta
     7021 tcgtcttttg gtctatcatc ttatgatgct agccaattaa cagctacaaa agcaacttct
     7081 gatttctttg aaaaagctgt tgctattggg ggtgatgcaa aacaagtttc caattggtta
     7141 caaggagaag tggcgcaatt tcttaacagt gaatcaaaat caatcgaaga aattggtctg
     7201 actcctgaga atttggttga gatgattggc ttgattgctg acggtacaat ttcatccaaa
     7261 attgctaaaa aagtttttgt gcatttagct aaaaatggtg gttcagctga agaattcgtt
     7321 aaaaaggctg gtttagttca aatttcagat cctgaagtat taatcccaat tattcatcaa
     7381 gtctttgcag ataatgaagc tgctgtgatt gactttaagt caggaaaacg caatgctgat
     7441 aaggccttca caggttatct aatgaaagca acaaaaggac aagctaatcc acaagttgct
     7501 ctgaagctgt tagcgcaaga attagcaaaa ttaaaagaag aatagaaatc agccttttgg
     7561 ctggttttta ttcgctaatt tagcacgaac acctagggat aaataattaa gaagctaaga
     7621 tatttatcag atgactttaa ctacagtgtt taagaaataa aaatgagggg aaaaatgact
     7681 aaaaaagaaa aaggaacaat gatgacctta gctgccggac tagcatgggg catttctgga
     7741 attagtggtc aatacttgat gtcgcacgga gtacacgtta accttttgac ttctttacga
     7801 ttgttgataa caggtatctt tctcttgtct ttagctagat caaaacaaaa ggagcattta
     7861 gtagcagcgt ggaaacagcc aaaattttta aagcaggttt tacttttttc tatatttggt
     7921 ttagttttaa atcaatatgc ttttctcaga gcgattcatt taacaaatgc tggaactgca
     7981 acggttttgc aatatatggc accaatactt attttgagta ttgtttgtat cctaaacaga
     8041 caaagaccaa catcttttga aataatagca attgctatgg caattttagg aacctatatg
     8101 attgcaacgc atgggaagtt aggaagttta gctatcacac cgaaaggatt aatgtggggg
     8161 cttggttccg ccattactta ttctatttat attttattac ctgtgaaact tattcatgaa
     8221 tggggaagta cgattgttat tggttcagga atgtttatag gtgggatttt atttagtttg
     8281 gtgacaaaag cgtggcaata ccccttacaa attaatgtga tgagtatact tgcttatatt
     8341 ggcattattg gtattgggac tatttttgct tacacttttt tccttaaagg ggttagtatt
     8401 gtaggtgctg tcaagggtag cttattagca tcggtagagc cagtttcttc ggtatttttg
     8461 acagtacttg ttttaggaga aattttttat ccaatagatt tacttgggat gctgtttatt
     8521 tttcttgctg taactctaat ctcttacaaa gatttaattg cattgaagaa aatgaaacga
     8581 gtaagatagt atcttttggg gttgaaaccc taatttaaag gtctttaaag accttttttc
     8641 tttactcgct atctcttaag aattatggta gaatgaatag taaaatatat tttttggaga
     8701 tgaaattgag tatagatgat tataggccga ccttcgtggt tgaggcagtt tatgatttaa
     8761 gagcagaaga tttattacgt catggcattc gtgcggtttt agttgattta gataatacac
     8821 tcattgcttg gaataatcca gatggtactg ctgaggtgcg tgcatggtta gatgagatga
     8881 caacagcgga tatttcagtt gtagtagtgt ctaataacaa tcatgctcgc gtagaacgtg
     8941 cagtatcacg ttttggtgtg gattttgtaa gtcgtgcgat gaaaccattt actcgtggta
     9001 taaacatggc aattgagcgt tatggctttg atcgtgacga ggtgattatg gttggagatc
     9061 aattgatgac agatattcgt gctagtcatc gcgcaggaat aaagtccgta ttggtaaaac
     9121 caattgttaa atctgatgct tggaatacca agttcaatcg cttacgtgaa cgtcgcgttt
     9181 ggaaaaaaat agaagagaat tacggtaaaa tcgtatatca aaaaggaatt taatggaaga
     9241 gttattttgt attggctgcg gagccaggat tcaaactgaa aataaagatg ctgctggcta
     9301 tacacctcgt gcagcacttg aaaaaggttt agagacagga gaattatact gtcagcgttg
     9361 tttcagattg cgccactata atgagattac tgacgttcat atcactgatg atgaattttt
     9421 gaaattacta catgaggttg gagatagtga tgcccttgtg gtgaatgtca ttgatatttt
     9481 tgatttcaat ggttcaatta ttcctggtct tagtcgtttc gttgcaggaa acgatgttct
     9541 tttagtagga aataaaaaag atattcttcc caaatcggtt aaagatggaa aagtaacaca
     9601 atggttaact gagagagctc atgaagaggg gcttcgtcct gttgatgtca tcttgacaag
     9661 tgcccaaaat catcatgcta ttaaggattt gattgatacg attgaaaagt atcgtcatgg
     9721 tcgagatgtt tatgttgttg gggttactaa tgttggaaag tcaactctta ttaacgctat
     9781 tattcgtgaa attactggta gtcgtgatgt tattacgacc tcacgtttcc caggtacaac
     9841 gttagataaa atagaaatac ctcttgatga tggtagctat atttttgata ccccgggtat
     9901 tattcatcgt catcaaatgg ctcattattt gacagcaaaa aatcttaaat atgttagtcc
     9961 taaaaaagaa ataaaaccca aaacttatca gcttaattca gaacaaacat tattcttggc
    10021 tggcctagca cgttttgatt ttatttctgg tcaaaaacaa ggttttacgg catattttga
    10081 taataacctg aatttacacc gcactaaact agttggagca gatgaatttt acactaaaca
    10141 tgttggaaaa ttattgacac caccaacagg taaggaagtt tcagattttc ctaaattagt
    10201 tcgtcatgaa tttacaatca aagataaaat ggatattgtt tattcgggtc ttggttggat
    10261 acgtgtgaaa tcagaagcag aaaatccagt agtagtagct gtttgggctc cagaaggcgt
    10321 ggcagttgtt ttacgcaaag ctttaattta ataactaaat taaccagcaa catatgacgg
    10381 tttttcatct aatttagctg atgatacagt cataatccta taatagataa aaaggaaact
    10441 aatatgttaa catctaaaca acgtgctttc ttaaaatcag aagcacattc tatgaaaccc
    10501 attattcaaa ttgggaaaaa tggtttaaat gatcaaatca aaacaagcgt acgtaatgca
    10561 ctcgatgcac gtgagctaat caaagtaacc cttttacaaa ataccgatga agatatccac
    10621 gatgtcgctg aagttctgga agatgaaatt ggctgcgata ctgtccttaa aataggacgt
    10681 atcttgattc tttataaaga gtcagcgcgc aaagaaaatc gtaaaatttc agttaaagta
    10741 aaagcagtaa aatcaaacta aactacaata gattaagaaa tagagtatag aactaaaaaa
    10801 cgatttacct aagttacact tccgtatgat agctgatcat gataatcagt gtttgttttt
    10861 ttagagatac tacttgttca tggaggaatt atggcactcg aattgctaac accatttaca
    10921 aaagttgaat tagaagagaa aaagcgagat actaaccgaa aacaaattgg aattatggga
    10981 ggaaatttta accctgttca taacgcccac cttgttgttg cagatcaagt acgtcagcaa
    11041 ttgtgtttag accaagttct gttgatgcct gaatttcagc caccacatat tgataaaaaa
    11101 gagacaattg atgaacagca tcgtctcaag atgcttgaat tagcaataga aggcattgat
    11161 ggtttatcta ttgaaccaat tgaaattgaa cgtaaaggta tcagctatac atacgatacg
    11221 atgaagcttc taattgaaaa aaatccagat gttgattatt atttcattat tggggcagat
    11281 atggtggaat atctacctaa gtggcatcgt attgatgaac ttgttaaaat ggtgcagttt
    11341 gttggtgttc aacgtccaaa atataaagct ggtacatcat atcccgttat ttgggtggat
    11401 ctgccattga tggatatctc atcaagtatg attcgtcaat ttattaaaag taatcgtcag
    11461 cccaactatt tactgcctag agaggtatta gattatatca ggaaagaagg tctttacaaa
    11521 tgacctacaa agattacaca ggtttagatc ggactgaact tttgagtaaa gtgcgtcata
    11581 tgatgtccga caaacgtttt aatcatgtgt taggagtaga gcgtgctgca atagagttag
    11641 cagaacggta tggttatgac aaggaaaaag cgggtctagc tgctttatta catgattatg
    11701 ctaaggaact atctgatgat gaatttctta gattaattga taaataccaa ccagatcccg
    11761 acctaaaaaa atggggtaat aatatttggc atggtcttgt tggcatttat aaaatacaag
    11821 aggatctagc tatcaaagat caagatattt tagctgctat tgcaaaacat actgttggtt
    11881 cagcccaaat gtccacattg gataaaatcg tttatgtagc agattacatt gaacacaatc
    11941 gagattttcc aggagttgaa gaagctcgtg agttagcgaa agttgacctt aataaagcag
    12001 tagcttatga gactgctaga acagttgcgt ttttagcaag taaggctcag cctatctatc
    12061 ctaaaacgat tgaaacctat aatgcttata taccttatct cgactaattc ttttattgaa
    12121 taatggtcat gataggtttg gcgatgcagt ggtcattttg gaggaaagta tgacttgtgc
    12181 acttttacta attgatatcc aacaaggtat tatggataag aaaccaaagc atttaacaaa
    12241 ttttgcagtg cttttagatg atttactttt aagtgccaaa ggttctaatt gtgaagtgat
    12301 atggataagg catcatgata aggaattgcc tcaaggttct ccacagtggg aaatttggga
    12361 acagcgacat cttgtgacgc atcacaaaat aattgataaa acttacaata gttgttttaa
    12421 agatactcat ttacatgatt acttgcaaag taagcatatt agtcaattaa tcatgatggg
    12481 acttcaaaca gaatactgtt ttgatacatc agtaaaagtt gcttttgaat atggttatga
    12541 tatctttatt ccacaaggtg gacacctaac atttgataca ccaactttaa gtggggattc
    12601 tattaaaaaa cattacgaga atatttggca tcatcgtttt gcgacaatgg tagcaaaaga
    12661 tagcttattg aaattaataa acgaggaata aaatgacaga aaaagactta ctacagctag
    12721 tcgttaaggc tgcggatgaa aaacgagcag aagatattgt aattttagat ttacagcctg
    12781 taacgagtgt tgcagattac tttgtgatta tgagtgcaag taatagtagg cagttagaag
    12841 caatcgcaga caatatccgt gaacaagtaa aaggaaatgg tggggatgct agccaccttg
    12901 aaggcgatag taaagctggt tgggtacttc ttgacctcaa tagcgtcgtg gttcatatct
    12961 tctctgaaga tgagagacag cactataacc ttgaaaaact ttggcatgaa gcaccattgc
    13021 ttgatgctga agtctttatg acagaataaa gctaagcagt cttgggactg ctttttgaga
    13081 gatgtaatat caagaaagat ttgagaacta ttatatggca acgtatgaaa cttttgcagc
    13141 tgtttatgat gctgtcatgg atgatacttt atatgctaaa tggactgatt tttcacttcg
    13201 tcattttcca aaaggtaaga aaaaactttt ggaattagca tgtggtacgg gaattcagtc
    13261 tgttcgattt gcacaagctg gttatgctgt tacaggactt gatcttagtg gcgatatgct
    13321 gaaacttgcc aaaaaacgtg caacttctgc tcaccaaagt atccagttta ttgaaggaaa
    13381 tatgttagat ttatctaatg ttggtaaata tgatctcatc acgtgctatt cagactctat
    13441 ttgttatatg caagatgaag tcgaggttgg tgatgttttt atcgaagtct ataaggcctt
    13501 agaagaaaat ggcgtcttta tttttgatgt tcattcgact tatcaaacag ataaagtatt
    13561 tcctggttat tcttatcatg aaaatgcaga tgactttgct atggtgtggg atacttatga
    13621 agatgatgca cctcattcta ttgtccatga attaacattt tttgttcaag aagaagatgg
    13681 tcgatttaca cgtcatgatg aagttcatga agagcgtact tatgatattt tgacttatga
    13741 tattttactt gaacaggcag gttttaaaga tgttaaggtg tatgcagatt ttgaagataa
    13801 aaaaccaact gcaacttctg ctcgctggtt ttttgtagct cataaataag taataatttg
    13861 gaggtaaaaa gatgataata ttcttatacg cttgtctgat gatatttggg atactttata
    13921 ttattgaacg tcgggtaagt cagaaaaagt ctaaagttat tttggaatta ttgatgaacc
    13981 ctaaacttca tttggattta ttatcggcat taaaaccatt tgaaaaagaa acagatcgtc
    14041 tcatatacct tcgtaaaact tatcacctag atattattga tgctgttact ttaaataatc
    14101 aattgaaaga gaaagaagta tgacagttac aggaattgtt gccgaattta atccctttca
    14161 taatgggcat aagtaccttt tagaacaagc tcaaggcata aaagtaatag ccatgtcggg
    14221 taattttatg caacgtggtg agccggcgat tgtagataaa tggacccgtt cccagatggc
    14281 actagaaaat ggtgctgatt tagtgattga actccctttt ctggtttcgg tacagtcagc
    14341 ggattatttc gctagtggag ctgtgtctat cctggcacga ttaggtgtgg ataacttatg
    14401 cttcggtacc gaagaaatgc ttgattatgc ccgtattggg gatatttatg tgaataaaaa
    14461 agaggagatg gaagcttttt tgaaaaaaca atcggattct ctttcctatc cacaaaaaat
    14521 gcaagctatg tggcaagaat ttgcagggat tacattttcg ggacaaaccc caaatcacat
    14581 tttaggctta gcctacacca aagcagctag ccaaaatggt attcgattaa atccgataca
    14641 acgtcaagga gctggttatc attcatcaga aaaaacagag atatttgctt ctgcaacaag
    14701 tcttcgtaaa catcaatctg atcgtttctt tgtagaaaaa ggtatgccta acagtgactt
    14761 gtttttgaat agtccacaag tagtttggca agattatttt agtttactca aatatcagat
    14821 aatgacgcat tctgatttga cacaaattta tcaagtgaat gaagagatag ccaatcgtat
    14881 aaaaagtcaa attcgttatg tggagactgt ggatgagtta gttgataaag tcgcaactaa
    14941 acggtatacc aaagcaagaa ttcgacgttt attaacctat atcttgataa atgctgtgga
    15001 aagtcctata ccaaatgcta ttcatgtctt aggttttaca caaaaaggtc aacagcattt
    15061 aaagtctgtg aagaagtctg tggatatcgt gacgaggatt ggttcacaaa cgtgggatag
    15121 tttgacgcaa cgagcagatt ctgtttatca gatgggaaat gctaatatag cagagcaaac
    15181 atggggacgt atcccttttt atcaacctgt tagtcaaagt gacctttgat ttaagcttta
    15241 taaattaatc atcacaaaat aatatttttc ccttgaccct tacttaacgt tatacattat
    15301 actctttaca gagaggttat tatgaaaaca gttaaagaaa taagtcacat ttctggtatt
    15361 agtgtgcgca cacttcatta ttatgatgaa atagatttgt tatctccttc atttgtagga
    15421 gagaatggtt atcgttacta tgatgatgaa tcactgatta agttacaaga aattttgtta
    15481 tttaaggaac tggaatttcc tcttaaaaaa ataaaagaga taatggatag tccaaattat
    15541 gatcgtaatc aagcacttct tgatcaaatt aggtggctgg aactaaagaa acagcgacta
    15601 gaagaagtaa ttgaacatgc aaagtctatc cagaggggta aaaatatgtc agattttaca
    15661 gcttataatc aagaagaatt agaagcattt caagaggaag ctagaacacg ctggggggat
    15721 acagatagtt acaaagaatt tgaaaatagc catagtaaga atgatttttc aatgatttca
    15781 caagctatga gtcagatttt taaagacttt ggacagttga aagaactttc tccgacagat
    15841 gagaaatttc aaaaacaagt tcagatttta caagattata tcacagctca gttttacaac
    15901 tgcactaacg atttattagc aagtcttgga ataatgtaca tccaagatga gcgtttccaa
    15961 aagtctattg ataattgggg aggccaggga acagcattat ttgtttctaa agctatagat
    16021 agctattgtc attaggctat attttaggtt tgcaatgaaa tgataggaat caaggaacag
    16081 tagatattat taaaaatgtc agactcttat ctaaattttt gacagtaagg agcaaaaaga
    16141 gtagactgta ggcatagaaa ggtaggtatc gttatgaaag gatttcatgg gaatatcgaa
    16201 aaattgacac taggaaatac aaatttccgc caagtcctct ataccgcaga acattgtcaa
    16261 ctcgttttaa tgacactgcc tgttggggga gaaattggta gcgagattca tgcagaaaat
    16321 gatcagtttt tccgttttga agctggtcat ggtaaggttg tcattgatgg taatgaatat
    16381 gaagttgcag atggagacgc tataatagtt ccggcaggtg ctgaacataa cgtaattaac
    16441 acttctgaaa ctgagatgtt aaaactctat acaatttaca gtccagctca tcacaaagat
    16501 ggtattatcc gtgccacaag agaagaagct gaagaaaatg aagaagattt tgatggagtt
    16561 actactgaaa ctaaataata ctagactaaa aagcgctatg tcaatagcgc tttttagctt
    16621 aatagtagtc atttcttgga gaatataaat agtaatttag gaagtaatgt taataaccga
    16681 gcttttaagg aaacgttaag taagggcggt tatactataa acatcctaaa cttttttcat
    16741 aaaatctcca gaaagaacac taagtatttt ggtgtttttt ctattttgaa agcatactgc
    16801 aactatgaaa aaccagaaag tattaacttt ctggtttttt agttaaacta aagtcaaagc
    16861 aatattgtat aagatagccc caaggacacc gccgataatt ggacctacaa ctggaatcca
    16921 agcgtatgac caatctgagt tacccttatt ttcaataggt aagatagcat gcataagacg
    16981 aggccctagg tcacgtgcag ggttaatagc ataaccagtt gttggaccaa gagagaatcc
    17041 tactgcgaaa ataactatac caacgataat tggaccaaga cctgctgcaa ctttactagg
    17101 accaattgcc ataattgtaa tcacaagaat agcagtaccg ataatttcac caaggagatt
    17161 cgatggtgta tggcggatag caggtccggt tgagaagctg gcaaggataa gtccactatc
    17221 ttttgtttct ttccaatgag gataatagtg taaccataaa atagttgccg caaccattgc
    17281 tcctaacatc tgtgcaatga taaaggggat aacttgtccc caagaaattg ctccaataga
    17341 agccattgct aatgtgactg ctggatttag atgagcggga ctgaataatc cagaaacata
    17401 aacagcaacg gtacaagcaa ttccccagcc aaaaacaata gcagtccatc cagttccttc
    17461 ttctttggtt ttactgagca catttcctgc aactacgcca tccccaagaa gaaccaaaat
    17521 aaatgttcct aaaaattccc caagaaattg agtcataata tctccttatt tattgatagt
    17581 taattcagct aattgtgatt gaatactatc caggtcagca cctgcctgta gaagagcagc
    17641 agcggtgtaa gctccctcca ctactgggac agagttaatg ataatatttt tttctgaaaa
    17701 atcagctact aactctaaat tcatttttgc tgaacctaaa tcaaagaaag ctaataacgt
    17761 ctttttatca ttttgctcta caatctgttg aacttgatca aaagaagtac caatctcacc
    17821 atcttctgtt ccacccacgt aagttattga gacatccttt gctacctctg aaataagatc
    17881 aacaactcct tgagcaatat ttttagagtg agaaacaaca ataataccaa tatcagacat
    17941 tagacacctc caacttctag taatgcttta aataataatc ctgaagaaaa tgatcctgga
    18001 tcaatgtgtc ctactgagcg ttctccaaca taagaagcgc gtccttttgt tgctttcatc
    18061 ccttttgtag catctactaa ggaatcgata cggtcttcag tcaattcatt tttttcaatc
    18121 gcttcaataa caccgtgcca aacatcaacc attgtttttt cattaagttc agctttacca
    18181 cgtttttgaa tcatttcaag tcctgcgcca agtgcctctg aaatggtact tttactctgt
    18241 tcagcttttg taattcccat aaatgcagaa ccatagagtg gtccagatgc tccgcctact
    18301 ttgcttaaaa gttgcatgga cacggttttg aaaacatcgg cagcactact aaattcattg
    18361 ttatcaaggt tttcaataac agcagtcata ccacgcgcca tattgccacc atggtcgcca
    18421 tcacctattg gagtatctag ttcggataaa tagtccttgt tggattgaat tttttggtta
    18481 aaagtgtgca tccattctat tgcagttttt acttccataa gagttccttc cttttgttct
    18541 aattaacagg aatgattacc aagcaatggt tgtaacgtca ctattaagtg cttctaacca
    18601 ctcttgattt tccagtttaa tcaaggttag tgataaacca gccatatcaa ttgaagtcat
    18661 atagttacca agttttttgt aattcacttc aatgccttta tcctcaagga gtttggcaac
    18721 atcgttagca aagacatatt gttccataag tggggttgca cccataccgt taataaggac
    18781 accatatttt tcgccagatt tagcatcgaa cgattcaatt aacttatcaa ctaattctgt
    18841 cgctaaagcc tttgaaggtt gcattttttc tttacgataa cctggttcac catgaatacc
    18901 gataccaaat tcaatttcat cttctgcaag aacaaatcct ggcttgccta cttcaggaac
    18961 agtagccccg cttagtgcaa gtccaacagt gtgaatgttt ggtactaatt catcagcaat
    19021 agctttgatt tcctgaagtg atttgccatg acgagcagca tgtcctagaa ttttatggac
    19081 gagtatcgta ccagctacac cacgttttcc ttgtgtgtaa agactgtctt cgactgcaat
    19141 atcatcatca acgaccacac ttgcaacttc tatgccttcc atttcagcca tatcttgtgc
    19201 catttcaaag ttcatgatat cccctgaata atttttgata accatgaaaa ctccagcccc
    19261 ctcatcagct tctttaattg cttcaagaac ttggtcagga gttggagaag taaatactgc
    19321 accacacaca gctgcagata acattccttc gcctacgaaa ccagcatgtg aaggctcatg
    19381 tccacttcct ccgccagaaa tgagagccac ttttcctgac ttctcttcat tgcgagcaat
    19441 gatatcaaaa ccttctatgc gatgtactaa atcattatga acatatgcta atccatctag
    19501 catttcatta acaacatcag ttggttgatt gagaattttt ttcatttgga aactcctttt
    19561 atgtttaatt ctcttttatt ataaaatttg taagcctttt ctggaatgga cagattttct
    19621 tgtttgtccg ctttgttagg gttttcaata atcataaatc atgttacact aaaccataat
    19681 aaatcatcag caattcaata taaaataaag gagaaattat gacttcttcg cttattacta
    19741 agaaaaaaat agccaagtca tttaaacgtt tatttatttc gcaagctttt gacaaaatat
    19801 cggtgagtga tattatggaa gatgcaggta ttagacgtca aacattttat aatcactttg
    19861 ttgataaata tgctttatta gaatggattt tccaaacaga attaagcgaa caagttacgg
    19921 ataatttaga ctatatctca ggttttcagt tgttatcaga gttgttgact ttttttaaaa
    19981 tgaatcaaga attctatatt aaattatttc aaattgaaga tcaaaatgat ttttcttcgt
    20041 attttgagtc ttactgtgag cagttagtag ataagcttct ttctgattat agtaaatcta
    20101 actttaatca gaaagaaaga gttacattt
//
LOCUS       DATRNK010000037        19461 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.037, whole genome shotgun sequence.
ACCESSION   DATRNK010000037 DATRNK010000000
VERSION     DATRNK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19461
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.037"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..868)
                     /locus_tag="VBC52_001289"
     CDS             complement(<1..868)
                     /locus_tag="VBC52_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO6882876.1"
                     /translation="MNSLDNLLSHNLEDNRHLLPYEHMHTEVRNGYPDILFHWHPELE
                     ISYVHEGTARYHIDYDFFNSQSGDIILIRPNGMHSIHPIENKEHITDSIKFHLDLIGY
                     SIVDQVSLRYLQPLQTSSFKFIQCIKPSMTGYNDIKNCLFDIFNISKEENRHFELLLK
                     AKLNELLYLLYYHQYVIKKHTDDTYRKNERIRDLIDYINNNYQQNLTIEFLADYMGYS
                     KTHFMTVFKQHTGTSCTEFIIQVRLNKASEHLINSTTAIIDIANSVGFNNLSNFNRQF
                     KRYYHTTPRQYRK"
     gene            1493..2314
                     /locus_tag="VBC52_001290"
     CDS             1493..2314
                     /locus_tag="VBC52_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6882877.1"
                     /translation="MIKVIATDMDGTFLRSDKTYDKARFSSLLTLMEKYDIKFVAASG
                     NLYDQLLLNFLEYPNRIAYVAENGGRVIDQDGTLLKETYLSNDTVAAVLSYLYQNYPE
                     TLISLSGEKRSYLERRTPINRRTELEYYMPNFIYKDHLLPLDDDRYFQMTLWVNENLV
                     SEMLLDISEHFKNHHIRLTSSGFGCIDVLPADVNKADGIAILLEKWGLKQDQVMVFGD
                     GGNDVEMLRAANISYAMSNAPEEIKAIAKYQTVSNDQDGVLETIENFLHKMSDNE"
     gene            2415..3029
                     /locus_tag="VBC52_001291"
     CDS             2415..3029
                     /locus_tag="VBC52_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEO6882878.1"
                     /translation="MLEVIDKALRDYQMKREQFFEINNQTVQEGAIVFTGDSIVEFFP
                     LKKHLGRDYPLVNRGVAGSDTYWLLENLRTQVWELLPSKVFILIGTNDIGLGHSQSEI
                     IANITDIIAEIRAESYMTEINILSVLPVSEEDDYIERVKVRNNQAIKALNKTLSVISG
                     INYIELYDLLVDEKGQLASSFTKDGLHLTDQAYAKISETIKLHL"
     gene            3192..4628
                     /locus_tag="VBC52_001292"
     CDS             3192..4628
                     /locus_tag="VBC52_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEO6882879.1"
                     /translation="MVKQVFPKGFLWGGATAANQCEGAYNVDGRGLANVDVVPTGEDR
                     FAIISGQKKMFDFEEGYFYPAKESIDFYHHYKEDLALLAEMGFKTYRMSIAWTRIFPK
                     GDELYPNEAGLRFYENIFKECRKYGIEPLVTITHFDCPIYLIKHYGGWRSRKMIGFYE
                     RLVRALFTRFKGLVKYWLTFNEINMILHAPFMGAGLYFEDGENQEQIKYQAAHHELVA
                     SAIAVKIAHEVDPNNQIGCMLAAGQYYPNTCHPQDYWASMQKNRENYFFIDVQTRGKY
                     PNYAKKYFEHLGISIQMTAEDLALLRDYTVDFISFSYYSSRVASGNPTVSEQVQENIF
                     ASLKNPYLKSSEWGWQIDPLGLRITLNAIWDRYQKPMFIVENGLGAVDIPDENGYVED
                     DYRIDYLRQHIAAMRDAIYVDGVNLIGYTTWGCIDLVSAGTGEMEKRYGFIYVDRNNK
                     GEGTLKRYKKKSFYWYKKVIASNGSQIE"
     gene            4719..5852
                     /locus_tag="VBC52_001293"
     CDS             4719..5852
                     /locus_tag="VBC52_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO6882880.1"
                     /translation="MKFEKRQVYYVVITFAICYAIQAYWGAVSNILTTLHKAIFPFLM
                     GAGIAYIINIVMSVYERLYIKLFKGSRLLMAIKRSVSMILSYATFIGLIVWLFSIVIP
                     DLISSLSSLLVIDTGALAKLVNNLNENKQISEALNYMGTDKDLVSTLSGYSQQILKQV
                     LSVLTNLLTSVSSIAATLLNVFVSFIFSIYVLANKEQLGRQFNLLIDTYLGSTGKTFH
                     YVRHILHQRFHGFFVSQTLEAMILGSLTVIGMLIFQFPYALTVGVLVAFTALIPVVGA
                     YIGVTIGFILIATESLTEAFLFVLFLILLQQFEGNVIYPKVVGGSIGLPSMWVLTAIT
                     IGGALWGILGMLLAVPVAATIYQIVKDHIIKRQTLRNRARTYR"
     gene            5948..6628
                     /gene="radC"
                     /locus_tag="VBC52_001294"
     CDS             5948..6628
                     /gene="radC"
                     /locus_tag="VBC52_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEO6882881.1"
                     /translation="MYHIELKKEALLPRERLVDLGADRLSNQELLAILLRTGIKEKPV
                     LEISTQILENISSLADFGQLSLQELQSIKGIGQVKSVEIKAMLELAKRIHKAEYDRKE
                     QILSSEQLARKMMLELGDKKQEHLVAIYMDTQNRIIEQRTIFIGTVRRSVAEPREILH
                     YACKNMATSLIIIHNHPSGSPNPSESDLSFTKKIKRSCDHLGIVCLDHIIVGKNKYYS
                     FREEADIL"
     gene            complement(6641..7279)
                     /locus_tag="VBC52_001295"
     CDS             complement(6641..7279)
                     /locus_tag="VBC52_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEO6882882.1"
                     /translation="MIMDKSIPKATAKRLSLYYRIFKRFNTDGIEKASSKQIADALGI
                     DSATVRRDFSYFGELGRRGFGYDVKKLMNFFAEILNDHSTTNVMLVGCGNIGRALLHY
                     RFHDRNKMQISMAFDLDSNDLVGKTTEDGIPVYGISTINDHLIDSDIETAILTVPSTE
                     AQEVADILVKAGIKGILSFSPVHLTLPKDIIVQYVDLTSELQTLLYFMNQQR"
     gene            complement(7434..7781)
                     /locus_tag="VBC52_001296"
     CDS             complement(7434..7781)
                     /locus_tag="VBC52_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEO6882883.1"
                     /translation="MAFEKEINLPDCNYIYTISPNIKKYTLRDTTFTQNRLGNYELER
                     LLETVPNSGDGFPLKITINKELSGFKLLITDKSGLRIVNIFKSTDHKILQDKFYFLMD
                     SLVERDIFTKTAK"
     gene            complement(7783..8898)
                     /locus_tag="VBC52_001297"
     CDS             complement(7783..8898)
                     /locus_tag="VBC52_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6882884.1"
                     /translation="MIYLDNAATTALTPSVIEKMTNVMTSNYGNPSSIHTFGRQANQL
                     LRECRQIIAEYLNVNSREIIFTSGGTESNNTAIKGYALANQLKGKHIITSEIEHHSVL
                     HTMTYLSERFGFDITYLKPNHGQITAKDVQEALRDDTIMVSLMFANNETGDFLPIQEI
                     GQLLRNHQAVFHVDAVQVFSKMELDPHSLGIDFLAASAHKFHGPKGVGILYCAPHHFD
                     SLLHGGDQEEKRRASTENIIGIAGMSQALTDATTNTLKNWTHISQLRTTFLDAISDLD
                     FYLNNGQDCLPHVLNIGFPGQNNGLLLTQLDLAGFAVSTGSACTAGTVEPSHVLTSLY
                     GANSPRLNESIRISFSELNTQEEILKLAKTLRKIIGD"
     gene            complement(8902..9876)
                     /locus_tag="VBC52_001298"
     CDS             complement(8902..9876)
                     /locus_tag="VBC52_001298"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO6882885.1"
                     /translation="MAEQYADKQIKLFSLTANREIAEKISQASGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVVPYFGYSR
                     QDRIAASREPITAKLVANMLVKAGVDRVLTLDLHAVQVQGFFDIPVDNLFTVPLFAEH
                     YNQLGLSGEDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREEGYIIGEVEG
                     KKAIIIDDILNTGKTFAEAAKILERGGATEIYAVASHGLFAGGAADILESAPIREIIV
                     TDSVLSKERIPSNIKYLTASHLIADAIIRIHERKPLSPLFSYRSDKKD"
     gene            complement(10053..10625)
                     /locus_tag="VBC52_001299"
     CDS             complement(10053..10625)
                     /locus_tag="VBC52_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEO6882886.1"
                     /translation="MTHLEIEYKTLLNKDEFNRLTSLFSHVQPITQTNYYFDTETFEM
                     KAHRMSLRIRTLPNRAELTLKIPREVGNLEHNHGLTLEEAKYIVKNGQFPEDTEIASL
                     ILEKGVDPTKLVVFGQLTTTRREMETSIGLMALDSNIYADIKDYELELEVKQPKQGKR
                     DFDQFLKENNINFKYAKSKVARFSATLKNN"
     gene            10735..11406
                     /locus_tag="VBC52_001300"
     CDS             10735..11406
                     /locus_tag="VBC52_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO6882887.1"
                     /translation="MSMDWETFLDPYIQTVGELKIKLRGIRKQFRKQNRHSPIEFVTG
                     RVKSVESIQEKMVLRGISEENLAQDLQDIAGLRIMVQFVDDVDEVLALLRKRHDMTVV
                     QERDYITHMKSSGYRSYHVVVEYPVDTIDGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPEEIKQRLEKTAKIALELDEEMRKIREDIREAQLLFDPLNRKLSDGVGNSDDT
                     DEFYR"
     gene            11381..12217
                     /locus_tag="VBC52_001301"
     CDS             11381..12217
                     /locus_tag="VBC52_001301"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEO6882888.1"
                     /translation="MTQMNFTDRATRVAIIANGKYQSKRVASKLFAAFKHDPDFYLSK
                     KDPDIVISIGGDGMLLSAFHMYEKQLDKVRFVGVHTGHLGFYTDYRDFEVDTLINNLK
                     NDEGEQISYPILKVTITLEDGRVIRARALNESTIKRIEKTMVADVVINQVVFERFRGD
                     GILVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVYRTLGSSVIIPKKDAIE
                     IVPKRVGVYTISIDNKTVHYKNVTKIEYSIDEKSINFVSTPSHTSFWERVNDAFIGEP
                     EH"
     gene            12214..13098
                     /locus_tag="VBC52_001302"
     CDS             12214..13098
                     /locus_tag="VBC52_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6882889.1"
                     /translation="MKFEYIAEERCKVKTLLKSHDVSRGLLAKIKYRGGKIFVNGEEQ
                     NAIFLLEIGDVVTIDIPDEPSHETLEPVPHDLDIVYEDDHFLILNKPFGFASIPSSIH
                     SNTIANFIKHYYVSNNYANQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQAKAIE
                     KRYYALVSGSGDLADSGDIIAPIARDVDSIITRRVHESGKYAHTSYQVVARYGDVRLV
                     DIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRMDLGINRQALHCHSLSFYDPFMGKIN
                     KQTLDLTDDFDSVIMELH"
     gene            13124..14116
                     /gene="pta"
                     /locus_tag="VBC52_001303"
     CDS             13124..14116
                     /gene="pta"
                     /locus_tag="VBC52_001303"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEO6882890.1"
                     /translation="MRSLFGGLREKVASDYVKIVFPEGSDERVLRAAARLKFEGLVDP
                     VILGKADEVHDSLARLGFVDQDYSVIDPEQYEKFEEMKEAFVEIRKGKATMEDADRLL
                     KDVNYFGVMLVKLGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNREST
                     QERYIFADCAINIDPNAQELAEIAVNTADTAKIFDINPKIAMLSFSTKGSAKAPQAEK
                     VQEAAKIAKDLSPELAVDGELQFDAAFVPETAEIKAPNSDVAGKANVFIFPDLQSGNI
                     GYKIAQRLGMFEAIGPILQGLNAPVNDLSRGSSAEDIYKLAIITAAQAIDEKSK"
     gene            14178..14939
                     /locus_tag="VBC52_001304"
     CDS             14178..14939
                     /locus_tag="VBC52_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO6882891.1"
                     /translation="MISRKVALVTGASAGFGAAIVTKLVSDGYSVIGCARRMDKLKCL
                     GEKFSEGYFYPLQMDITSRESVDKALESLPKNLQSIDLLVNNAGLALGLDKSYEADFE
                     DWMTMINTNVVGLIYLTRCILPKMVEVNRGLIINLGSTAGTIPYPGANVYGASKAFVK
                     QFSLNLRADLAGTKIRVTNLEPGLCEGTEFSTVRFKGDHKRVEKLYEGAHAIQAEDIA
                     NTVSWVASQPEHININRIEIMPVSQTYGPQPVYRY"
     gene            15074..17074
                     /locus_tag="VBC52_001305"
     CDS             15074..17074
                     /locus_tag="VBC52_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEO6882892.1"
                     /translation="MQHVNHSSFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GISSVTESFILGSISLIIWTLTLITTIKYVLVALKADNHHEGGIFSLYTLVRKMTPWL
                     IVPAVIGGATLLSDGALTPAVTVTSAVEGLKVVPSLQHIFQNQSNVIFATLFILLLLF
                     AIQRFGTGVIGKLFGPIMFIWFAFLGISGLLNSFAHPEVFKAINPYYGLKLLFSPENH
                     KGIFILGSIFLATTGAEALYSDLGHVGRGNIHVSWPFVKVAIILSYCGQGAWILANKN
                     AGNELNPFFASIPSQFTMHVVILATLAAIIASQALISGSFTLVSEAMRLKIFPQFRST
                     YPGDNIGQTYIPVINWFLFAITTSIVLLFKTSAHMEAAYGLAITITMLMTTILLSFFL
                     IQKGVKRGLVLLMMIFFGILEGIFFLASAVKFMHGGYVVVIIAVAIIFIMTIWYKGSK
                     IVSRYVKLLDLKDYIGQLDKLRHDHRYPIYHTNVVYLTNRMEGDMIDKSIMYSILDKR
                     PKKAQVYWFVNIKVTDEPYTAEYKVDMMGTDFIVKVELYLGFKMRQTVSRYLRTIVEE
                     LLESGRLPKQGKTYSVRPDSKVGDFRFIVLDERFSSSQNLKPGERFVMLMKSSVKHWT
                     ATPIRWFGLQFSEVTTEVVPLIFTANRGLPIKEKIELTTTGD"
     gene            complement(17145..17816)
                     /locus_tag="VBC52_001306"
     CDS             complement(17145..17816)
                     /locus_tag="VBC52_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882893.1"
                     /translation="MAFASNESENNFFITLKTYFNYLFSIQIITDISTLNHADFDGSF
                     AFHDIETSIPHLVIDSNYLAISQTNSKTEANDIKTFSELSKTMTEFHYMLNFDLFNHL
                     PYRFRLHNKDGQTIYSNHKPEDPFDIYPEEEYPIDKWVQNSLIEKKAKELHLLLPGAS
                     QDYILVQSYKRLENDSGQLVGYIEHVHNIKPLLEGYLKESGQAIVGWSDVTSGASISN
                     DDFEL"
     gene            18108..19448
                     /locus_tag="VBC52_001307"
     CDS             18108..19448
                     /locus_tag="VBC52_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6882894.1"
                     /translation="MTKKGWRIMPAIITTAILGFSGILIETSMNVTFPLLMKEFGVNP
                     AVIQWVTTGNLLAVAVTVPLSAFMIKNLSERQIFTLANVLFLSGVLIDSFAPNLAILL
                     VGRVLQGVGTGLALPLLFHIILTQIPMERRGLMMGVAAMVTLLAPAVGPTYGGVISGM
                     LGWKMIFMLLAPILIISTFIGLASIPKRQVRINDKLNFPAFVSLGIGLATLLLAIEKM
                     SIFYLLVAIVSFVIFYYLNKQLEFLNLNVFKDKDFSILLYGVLAFQMIPLALSFLLPN
                     LLQLVLHQTSTKAGLFMFPGAIAVVFLSPFAGYLLDKIGAFKPIMIGISLSLIGLIGT
                     AIFIPAKFVVVLLAFDILTKIGMGIGASNMVTTALTKLKPAQSADGNSILNTLQQFAG
                     AFATAVASQIFTIGQVAIPKNGAIIGSQFAVLFVIIVVILAIVGLTYLRKRKAI"
BASE COUNT         6215 a         2978 c         3728 g         6540 t
ORIGIN      
        1 gtttgcggta ctggcgagga gttgtatgat aatagcgttt aaattggcga ttaaaatttg
       61 atagattatt aaaaccaacg ctgttggcaa tatcaataat ggccgtagtg gagttaataa
      121 gatgctcact agctttatta agacgaactt gtatgataaa ttctgtacag gatgttccag
      181 tatgctgttt aaatacggtc ataaaatgtg tcttactata ccccatataa tcagctaaaa
      241 attcaatggt aagattttgc tggtaattat tattaatata atcaatcaga tcacgaattc
      301 tctcgttttt tctgtaggta tcatcagtat gttttttaat gacatattga tgataataga
      361 gtaaatacaa gagttcgttt aatttagctt ttaggagtag ctcaaaatgt cgattttctt
      421 cttttgaaat attaaaaata tcaaacaaac aatttttgat atcattatat cctgtcatac
      481 taggtttgat acattgaata aacttaaaag agctagtctg caacggttgt aaataacgaa
      541 ggctaacttg gtctacaata gaatatccta ttaaatctaa gtgaaattta atgctatctg
      601 tgatatgctc tttattttca atcggatgaa tagaatgcat accatttgga cggatcaaga
      661 taatatcacc agattggcta ttaaaaaaat cataatcgat gtgataacgt gcggtcccct
      721 cgtggacata tgaaatttca agttccggat gccagtgaaa taaaatgtca gggtaaccat
      781 ttctaacctc agtatgcatg tgttcatagg gtaataaatg ccggttatct tctaaattat
      841 gtgatagtaa attatcaagt gagttcatca tttttctttc taaaatcgta ggatagtatt
      901 attataacag aaaactatca aaaaatgggt ttcgtaggat agtattattt tttgaaagga
      961 tagtgtaagt tttgtatagg aacatgacat ataatataag tcatagagaa aacgctttaa
     1021 aatttttgct tagagtgttt ctaataactt caaaatcctt acagggtcgt ttaatctcct
     1081 tatctcatgt aaggattttt tgttgtatta atatgaccaa atttattaat atactcaatt
     1141 atttattttt tcaaaacaga ataactatgt gaaaaaaaat aatgttaggt ctctcaaaaa
     1201 cacgtatagc aatttcgaat ctcaaatatt ttattatgac tattgtaata gttacgaatc
     1261 tattattcaa ctcaacaata agaaaactct tcagtaaatc attatgttaa acaagctctg
     1321 atagtatgga gataagtata ggtaaaagtc gatttaatct ctgaaacaaa atttaactta
     1381 attaatagaa ccgttgacgt atcaggtatc aaatcttttt ggtttatatg tgatataatg
     1441 aaagaacata ttgagatatt tggcaccctc ttgaaaggca aaggttactt gtatgattaa
     1501 agtaatagca acagatatgg atggaacttt tctacgctca gataaaactt atgataaagc
     1561 acggttttct tctttattaa cgttgatgga gaagtatgat attaaatttg tagctgctag
     1621 tggtaattta tacgatcaat tgttgttaaa ctttttggaa taccctaatc gtatcgccta
     1681 cgtcgctgaa aatggtggtc gtgtcattga ccaagatggc acattactaa aagaaactta
     1741 tttatctaat gatacagttg cagctgtttt atcctattta tatcagaact atcctgagac
     1801 actcatcagc ttatctggtg aaaaacgttc ataccttgag cgaagaactc ctattaatag
     1861 gcggactgaa ttagaatact atatgcctaa ttttatctat aaagaccatt tgctgccctt
     1921 agatgatgac cgatattttc aaatgacact ttgggtaaat gaaaatcttg tttctgagat
     1981 gttgttagat attagtgaac actttaaaaa tcatcatatc agattgacct ctagtggttt
     2041 tggttgtatc gatgtcttac cagctgatgt caataaagcg gatggtatcg ctattctcct
     2101 tgaaaagtgg ggattgaaac aggatcaagt gatggttttt ggtgatgggg gcaatgatgt
     2161 ggaaatgttg agagcagcaa atatttctta cgctatgtca aatgcgccag aagaaattaa
     2221 agctattgct aaatatcaaa cagttagtaa cgatcaagat ggtgtgttag aaacgataga
     2281 gaactttctt cacaagatga gtgataatga atagaaccac gtagcttttc acgaagcata
     2341 ctattcaatt ctcaggtgaa attttgttat aatacaagaa agcctagaga aatatttaag
     2401 aatgaaggag aacgatgtta gaagttattg acaaagcttt aagagattat caaatgaaac
     2461 gagaacagtt ttttgagatt aacaatcaga cagtacaaga aggtgccatt gttttcacag
     2521 gagattctat cgttgagttt tttcctttaa aaaaacattt aggacgtgac tatcctttag
     2581 ttaatagagg agtagcagga tcagatactt attggctttt agaaaacttg agaacccaag
     2641 tatgggaact attaccatcc aaagtattta ttttaattgg aacaaatgat attggtttag
     2701 ggcattcaca gtcagaaatc atagctaata tcacggatat aattgcagaa attcgtgcgg
     2761 aatcttatat gacagagatt aatatactga gtgttttacc tgttagtgaa gaagatgact
     2821 atatagagcg tgtcaaggta agaaataatc aagcaataaa agctttaaac aaaaccttat
     2881 cagtaatttc tggcattaat tatattgaat tgtatgattt attagttgac gaaaaaggtc
     2941 agcttgctag cagttttaca aaagatggtt tgcacctaac agatcaagcc tatgctaaaa
     3001 tttctgaaac gataaaactc cacctttaaa agtgtttaga tactattttt aagtatttaa
     3061 atacacagaa ttagtgaaaa gaccttctaa gtagaaagtt catgtcacgg atagtaccag
     3121 tatttggcga atcaatgtca gtaactggtc ttttttaata ttatgataag aaaaaacaag
     3181 aggggtaaat gatggtaaaa caagtatttc caaaaggttt tttatggggc ggggcaactg
     3241 ctgccaatca atgtgaagga gcttacaatg ttgatggacg tggcctagca aatgtagatg
     3301 ttgttcctac tggagaagat cgatttgcaa ttatttcagg acaaaagaaa atgtttgatt
     3361 ttgaggaagg atacttttat ccagcaaaag aatcaataga tttttatcat cattataaag
     3421 aagaccttgc attacttgca gaaatgggtt tcaaaaccta tcgtatgtca atagcatgga
     3481 cgcgtatttt tccaaagggt gatgagctat atccaaatga agctggtctt cggttttatg
     3541 aaaatatttt taaagagtgt cgtaagtatg gtattgaacc tttggtaacc atcacacatt
     3601 ttgactgtcc tatctacctt attaaacatt acggtgggtg gcggagccgt aaaatgattg
     3661 gtttttatga gcgccttgta cgagctttat ttactcgttt taaggggtta gttaaatatt
     3721 ggttgacctt taacgaaatc aatatgattt tacacgctcc ttttatgggc gctggcttat
     3781 attttgaaga tggtgaaaat caagagcaaa ttaaatatca agcagctcac catgagttag
     3841 tggcttcggc tattgcagta aaaattgctc atgaagttga tccaaataat caaatcggat
     3901 gtatgttagc tgcaggtcaa tactatccaa atacgtgtca tccacaagat tattgggctt
     3961 cgatgcaaaa aaatagagaa aattattttt ttattgatgt gcaaacgcgt ggcaaatacc
     4021 ctaattatgc taaaaaatat tttgagcatt taggtatttc aattcaaatg acagcagaag
     4081 atcttgcttt gttaagagac tatacggttg attttatttc attttcttac tattcaagcc
     4141 gagtagcttc aggtaatcca actgtcagtg aacaggttca agaaaatatt ttcgcatctc
     4201 ttaagaatcc ttacttgaaa tcttctgaat ggggatggca aattgatccg cttggattac
     4261 gcattacctt aaatgctatc tgggatcgtt atcaaaaacc gatgtttatt gttgaaaatg
     4321 gacttggagc agtagatatt cctgatgaga atggttacgt agaagatgat tatcgtattg
     4381 attacctccg tcagcacatt gctgcaatga gagatgctat atatgtagat ggtgttaatc
     4441 taattggcta tactacatgg ggatgtattg atttagtatc tgcaggaact ggtgaaatgg
     4501 aaaaacgcta tggtttcatt tatgttgatc gcaataataa aggagaggga acactcaagc
     4561 gttacaagaa aaaatcgttt tattggtaca agaaggttat tgcaagtaat ggcagtcaaa
     4621 tagaataaag agcagactaa taactgttaa ctctcttttt ctctttcata aaatagtgta
     4681 taataattga aagaaattaa gattaataag gagatttcat gaaatttgaa aaacggcagg
     4741 tctattatgt tgtcataaca tttgctattt gctatgctat acaggcttat tggggagctg
     4801 tttctaatat tttaactacg cttcataagg caatatttcc ttttttgatg ggagctggaa
     4861 ttgcctatat tattaatatt gtaatgtcag tctatgagcg attatatata aagcttttta
     4921 aaggatctag actattaatg gcaatcaagc gtagtgtttc tatgatttta tcctatgcaa
     4981 cttttattgg tttaattgtc tggctatttt caattgtcat tccagatttg atttctagtt
     5041 tgagttcttt attggttatt gataccggag cacttgctaa attggttaat aatctcaatg
     5101 aaaataaaca aatttctgag gctttaaatt atatgggaac agataaagac ttagtttcta
     5161 ctttaagtgg ttatagccag cagattttga agcaagtttt atctgtttta acaaatttac
     5221 taacctcagt ttcctctatt gcggcaacac ttctgaatgt ttttgttagt tttatttttt
     5281 caatttacgt tttggcaaac aaggagcagt tgggacgtca atttaatttg ttaattgata
     5341 cctatttagg ttcaacaggc aaaacattcc attacgttcg tcatatcctt catcaacgtt
     5401 tccatggttt ttttgtaagc caaactttag aagctatgat tttaggaagt ttgacggtta
     5461 ttggtatgtt gattttccaa tttccttatg ctttaacagt tggggtttta gttgctttta
     5521 cagctctaat accggttgtg ggagcctaca ttggtgttac aatcggtttc atcttaattg
     5581 ctactgaatc gcttactgaa gcattcttgt ttgttctttt cttgatcctt ttacaacaat
     5641 ttgagggaaa tgtcatttat ccgaaagttg tcggtggatc gattggactg ccttctatgt
     5701 gggttttaac ggctattact atcggaggtg ctttatgggg gatcttaggc atgttacttg
     5761 ctgttcctgt tgcagctact atctatcaga ttgtaaaaga tcatattatc aagcgacaaa
     5821 cgcttagaaa tcgtgcacga acctatcgtt aaataaaaaa tacttagatt tttcatctaa
     5881 gtatttttta ttgatttaaa acatgagttt ttgattggtt tacgaatatt atgactggag
     5941 gtgttttatg taccatattg aattaaaaaa ggaagcttta ctaccaagag aacgcctagt
     6001 tgatttaggc gcagatagat tgagtaatca ggagttatta gccattctct tacgtacagg
     6061 tattaaagaa aaacctgttc ttgaaatttc aacgcaaatt ttagaaaaca taagcagttt
     6121 agcagatttt ggtcaattat ccttacagga gttgcaatcc attaaaggaa tcggtcaggt
     6181 taaatccgtc gaaataaaag ctatgctaga actagcaaaa cggattcaca aagctgaata
     6241 tgatcgtaaa gagcaaattt taagtagtga acaattagcg aggaaaatga tgctcgaatt
     6301 aggggataaa aaacaagaac atttagtagc tatttatatg gatacacaaa atcgtattat
     6361 cgaacagaga actattttta ttggtactgt acgtcgttca gtagcagagc caagagaaat
     6421 tctacattat gcttgtaaaa acatggcaac ttctttgatt attatacata atcatccctc
     6481 aggttctcca aatcccagtg aaagtgattt aagtttcact aaaaaaataa aacgatcatg
     6541 tgatcatctg ggaattgtct gcctagatca catcatcgtt ggaaaaaata aatattatag
     6601 ttttcgagaa gaagcagata ttttataaat aatgaactat ttatcgctgc tggttcatga
     6661 aataaagtaa agtttgtaat tcgcttgtta aatctacata ctgaacaatg atatcttttg
     6721 gtaatgttaa atgaactgga gaaaaactca agatgccttt tatacctgct ttgactaaga
     6781 tgtcagcaac ttcttgggct tctgtactag gtactgttag gatagcagtt tcaatatcac
     6841 tatctattaa atggtcattg atagtcgaaa taccgtagac aggaatgcca tcctcggttg
     6901 ttttaccaac taaatcattg ctatctaaat caaaagccat tgaaatttgc attttattgc
     6961 gatcgtggaa acgataatgc aagagagctc taccgatatt tccacatccc accaacataa
     7021 catttgttgt agaatgatcg ttcaatattt ctgcaaagaa gttcataagt tttttgacat
     7081 cataaccaaa accacggcgt cctagttcac caaaataaga aaaatcacgt cgaacagtag
     7141 cagaatcgat acctagggca tctgcaattt gtttggaact agctttttcg atgccatcag
     7201 tattaaaacg tttaaaaata cggtagtaca gtgataaacg tttagcagtt gctttaggaa
     7261 tagacttatc cataatcaca aaattatctc cttgttaaaa attttattat tcgatttaga
     7321 attagttata taacttaatt cttcacatct ctattgtaca aaaagattgt gaaagtgaca
     7381 acaaaatggt tcgcaaaaag accactaaaa tgttaaaaat agtggtcgat aaattatttt
     7441 gcagttttcg taaagatatc acgctcaaca aggctgtcca ttaagaaata aaatttgtct
     7501 tgaagaattt tatgatcggt agatttaaag atatttacta ttctaaggcc agatttatca
     7561 gtaattaaga gcttaaaacc agaaagttct ttattgattg taatctttag aggaaagcca
     7621 tcgccagaat taggtactgt ttctaggaga cgctctaatt cataattacc taagcgattt
     7681 tgagtgaatg tagtatcgcg taaggtatat tttttaatgt tcgggctgat agtataaatg
     7741 taattgcagt ctggaagatt tatttctttt tcaaatgcca tattaatctc ctataatttt
     7801 tcttaaggtt ttagctaatt taagaatttc ttcttgggta tttagttctg aaaaactaat
     7861 acgtattgat tcatttagac gtggtgagtt ggctccgtac aagcttgtta agacatggct
     7921 aggttcgact gttcctgcag tacatgcaga acctgttgaa actgcgaatc cagctaaatc
     7981 taattgtgtc aataacaagc cattattctg tccaggaaaa cctatattaa gtacatgagg
     8041 taagcagtct tgaccgttat taagatagaa gtcaaggtct gaaatagcat ctaaaaaggt
     8101 cgttctcagc tgactaatgt gagtccaatt tttaagggtg ttagtcgtag catcagtaag
     8161 agcttgagac attccagcaa taccaattat attttcagtt gaagcacgcc ttttttcctc
     8221 ttggtctcca ccatgaagta gactatcaaa gtgatgggga gcacagtaaa gtatcccaac
     8281 accttttgga ccgtgaaatt tatgggcaga agcagctaaa aagtcaattc ctaaagaatg
     8341 aggatcaagt tccattttgc taaagacttg aacggcatca acgtgaaaaa cagcttggtg
     8401 gttcctgaga agctgaccaa tctcttgaat tggtaaaaag tccccggttt cattattagc
     8461 aaacatgaga gataccataa tagtatcatc tcgtaaagct tcttgaacgt cttttgcagt
     8521 aatttgtcca tggtttggtt ttaagtaagt aatatcaaaa ccaaatcgct ctgataagta
     8581 agtcatagta tgtaggactg aatgatgttc aatttcagag gtaataatat gtttaccttt
     8641 tagctgattt gcaagagcat accctttgat agctgtattg ttgctctcag ttcccccaga
     8701 agtgaaaata atttcacgtg aattaacatt tagatattca gcaataattt gtcgacattc
     8761 acgtaaaagt tgatttgctt gacgcccaaa ggtatgtata ctagatggat taccatagtt
     8821 acttgtcatg acattggtca ttttctcaat aacagatggg gttagagcgg tagtagcagc
     8881 attgtctaaa taaatcatca attaatcctt cttatctgaa cgatatgaaa ataatggact
     8941 taaaggtttt ctttcatgga tacggataat agcatctgca atgagatggc tagctgtcag
     9001 atatttaata ttgctaggaa tacgctcttt agataaaaca gagtctgtta cgataatttc
     9061 tctaattggt gcagactcta aaatatcagc tgcgccgcca gcaaaaagcc catgactagc
     9121 aacagcataa atctcagttg ctccaccacg ttctaaaatt tttgcagctt cggcaaaggt
     9181 ttttcctgta tttaaaatat catcaataat aatagctttc ttaccttcta cctcaccaat
     9241 aatatagcct tcttcacgtt cagaatcatc ttgtgcataa tcaatgatag caattggaga
     9301 gtcaaggtat tctgccaaac tacgagcacg cttgattcca gaatttttag gactaacaac
     9361 gacgacatct tcaccagaga gaccgagttg attatagtgt tcagcaaata gtgggacagt
     9421 aaaaagattg tcaactggga tatcgaagaa cccttgtact tgtactgcat gtaagtcgag
     9481 ggtaagaact cgatcaactc cagctttaac taacatatta gcgaccaatt ttgcagtaat
     9541 aggttcacga gatgcagcga tgcggtcctg acgagagtaa ccaaaataag ggactacgat
     9601 gttaacagta ttagcacttg cacgtttgca agcgtcaatc ataatgagta attcccagag
     9661 attatcattg actgggaagc ttgtagattg gatgatatag atatcgtcac cgcgcaccgt
     9721 ttcctcaata ttaatcataa tttcgccatc agagaattga cgtgatgaca tcttacccaa
     9781 aggaattcct gaagcttggg aaattttttc agcaatttct ctattagcgg ttaatgagaa
     9841 aagtttgatt tgtttatcgg cgtattgttc tgccatgaga tagatgactc ctttagattg
     9901 atactaatag tttaccaaaa aaatagaaaa aattcacgat aagaaacatg aaaatgcttg
     9961 caataacaat gttgaggaga gatagtatga gattttaagt tcttgcttaa atgagaatta
    10021 taaaagagat tggaaaatcc aatctctcaa cgttagttat tctttaaagt agcagagaat
    10081 ctagccacct tacttttggc gtatttaaaa ttaatattat tttcttttaa aaactggtcg
    10141 aaatctcttt tcccttgttt aggttgtttg acttctaact caagttcata atctttaata
    10201 tcagcatata tattgctgtc tagagccatt aagccgattg aagtttccat ctctcgtctt
    10261 gttgttgtaa gttgtccaaa gacaactaat ttggttggat caactccctt ttcaaggatt
    10321 aaagaagcaa tctcagtatc ttcagggaac tggccatttt taacgatata tttagcttct
    10381 tctaaagtaa ggccatgatt gtgctctaaa tttcccactt ctctagggat ttttagtgtt
    10441 agttcggcac ggttaggtaa cgttctaata cgaagtgaca ttcggtgtgc tttcatttca
    10501 aatgtttctg tgtcaaaata gtaattggtt tgagtaatag gttgaacatg agaaaataag
    10561 cttgttagac gattaaattc gtctttattc aatagagttt tgtattcaat ttctaggtgt
    10621 gtcatcttat cacttccttt tcctaaatat ggtataatat taattgttgt gttaatttta
    10681 tagaaaaaaa gtttatttta caagagagta cattaaatat tttggaggat tagaatgtca
    10741 atggattggg aaacatttct agacccttat attcaaacgg ttggtgaatt aaaaataaag
    10801 cttcgtggta ttcgtaaaca atttagaaag caaaatcgtc actctccaat agaatttgtt
    10861 actggacgcg taaagtcggt tgaaagtatt caagaaaaga tggttttacg tggtatttca
    10921 gaagaaaatc ttgctcagga cttgcaagat attgcaggtc ttcgtatcat ggtacaattt
    10981 gtagatgacg ttgatgaagt ccttgcctta ttgagaaaac gtcatgacat gacagtggtt
    11041 caggagcgag actatattac ccatatgaaa tctagtggat atcgttcata tcatgtcgtt
    11101 gtagagtatc ctgtagacac aatagatgga caaaagaaag ttttagcaga aatacaaatt
    11161 agaactctgg ctatgaattt ttgggcaacc atcgaacatt cattaaatta caagtatcag
    11221 ggggacttcc ctgaagaaat taaacaaaga ctggagaaaa cagcaaaaat cgctttagaa
    11281 ttggatgaag aaatgcgtaa gattcgcgaa gatattcgtg aagcacagct tttatttgat
    11341 cctttaaatc gaaaattgag cgatggtgta ggaaatagtg atgacacaga tgaattttac
    11401 agatagagca acacgtgttg ctataattgc taacggaaaa tatcaaagca agcgtgtagc
    11461 ctcaaagctc tttgctgctt ttaaacatga tcctgatttt tatttatcaa aaaaggatcc
    11521 tgatattgtc atttctattg gtggagatgg tatgcttttg tcagccttcc atatgtacga
    11581 aaaacaactg gataaagtaa ggtttgtcgg tgttcataca ggacatttag ggttttatac
    11641 agactatcga gattttgaag ttgacacatt aattaataat ttgaaaaatg atgaaggtga
    11701 acaaatctcc tatcccattt tgaaagtaac tattaccctt gaagatggtc gagttattcg
    11761 tgcgcgtgct ttaaatgagt ctactattaa gcgtattgaa aaaacaatgg tagcggatgt
    11821 tgttattaat caagtggtat ttgaaaggtt taggggagat ggcattttag tttctacacc
    11881 aaccggtagt actgcttaca ataaatcact agggggggca gtactccacc caacgataga
    11941 agccttgcaa ctaacagaaa tttctagtct taataatcgc gtctatcgta ccttggggtc
    12001 atcggttatc atccccaaaa aagatgctat agagatagtt cctaaacgag ttggtgtcta
    12061 tacaatttct atagataaca aaacggtcca ttacaaaaat gttaccaaaa tagaatatag
    12121 tattgacgaa aaaagtatta actttgtttc aactccaagt cacactagct tttgggaacg
    12181 tgttaacgat gcttttatcg gagaacctga acattgaaat ttgaatacat agcagaagaa
    12241 aggtgcaaag ttaaaacact tttgaaaagt catgatgttt caagggggtt attagcaaag
    12301 attaagtaca gaggtggtaa gatttttgtc aatggtgaag agcagaacgc tattttctta
    12361 ttagagattg gcgatgttgt tacgatagat attcctgacg agccttctca cgaaactttg
    12421 gaaccagtac ctcacgattt agatattgtt tatgaggatg atcatttttt gattttaaat
    12481 aaaccttttg gtttcgctag tattcctagc tctattcatt caaatacaat tgctaatttt
    12541 ataaaacact attatgtgtc taataactat gccaatcagc aggtccatat tgtgacccgg
    12601 ttggatcgag atacaagtgg attaatgtta ttcgccaagc atggttatgc ccatgctcgt
    12661 ctagataaac aacttcaggc taaagcaatt gaaaaaaggt attatgcatt agtatcaggg
    12721 agtggtgatt tagcagatag tggtgatatt atagctccta ttgcacgaga tgttgatagt
    12781 attattacga ggcgagtcca tgaatcaggc aaatatgcac atactagtta tcaagtagtt
    12841 gctcgttatg gtgatgttag attagtggat atcaaattac atacagggcg tactcatcaa
    12901 attagagttc actttgctca tatcggcttt cctcttttgg gagatgattt gtacggagga
    12961 cgtatggatc taggaataaa caggcaagca ttgcattgtc attctttgtc tttttacgac
    13021 ccttttatgg gaaaaataaa taaacagacg ctagacttga cagatgactt tgacagcgtt
    13081 atcatggaat tacattaatt tagaaataga aggttttata tatatgcgtt ccttatttgg
    13141 tggtttaaga gaaaaagttg caagtgatta tgttaagatt gttttccctg agggaagcga
    13201 tgaacgtgta ttacgtgcag cagctcgttt gaaatttgaa ggtcttgtgg atcctgttat
    13261 tttaggtaag gctgacgaag ttcacgattc tttggcaaga cttggttttg ttgatcaaga
    13321 ttatagcgtt attgaccctg aacaatacga aaaatttgaa gagatgaaag aagcttttgt
    13381 tgagattcgt aaaggcaaag ctactatgga agatgcagat cgtcttttga aggatgttaa
    13441 ctattttggg gttatgttag taaaacttgg tttggcagat ggtatggtat caggggccat
    13501 tcattcaaca gctgatactg tacgtcctgc tcttcaaatt attaaaacaa aaccaggaat
    13561 ttcacgcact tcaggtgtat tcttgatgaa ccgtgaaagt acacaagaac gttatatttt
    13621 tgctgactgt gctattaaca ttgatcctaa tgcacaagag ctagcagaaa ttgcagtaaa
    13681 tactgcggat acagccaaaa tatttgatat taatcccaag attgctatgc ttagtttttc
    13741 aacaaaagga agtgcaaaag cgcctcaagc tgaaaaagtt caagaagcag ctaaaattgc
    13801 caaagattta tctcccgaat tagctgttga tggagaatta caatttgatg cagcttttgt
    13861 tccggaaact gcagaaatta aagctcctaa tagtgatgtt gctggaaaag caaatgtttt
    13921 tattttccca gaccttcaat caggaaatat tggctataaa attgcacaac gtttgggtat
    13981 gtttgaagca atcggaccga tcttacaagg attgaatgct ccagtaaatg atttatcacg
    14041 tggatcaagt gctgaagata tttacaaact tgctatcatt acagctgctc aagctattga
    14101 tgagaaatca aaataagtta taaaacgaga aggatttttc ctagctcgtt tttctatata
    14161 ctagaggagg agtatttatg atatcacgta aagttgcgct tgtgacgggg gcatctgctg
    14221 gttttggtgc agctattgtt actaaattag tttcagatgg ttatagcgtt attggttgtg
    14281 ctcgtcgaat ggataaatta aaatgccttg gagaaaagtt ttcagagggt tacttttatc
    14341 cacttcaaat ggatattaca agtagagaat cggttgacaa agctttagaa agtctgccta
    14401 aaaatttgca atcaattgat ttgttggtta ataatgcagg tttagcactt ggattggata
    14461 aatcatatga agctgatttt gaagattgga tgactatgat taatactaac gttgtaggat
    14521 taatttactt gacacgatgc attttaccga aaatggtaga agttaataga gggttaatta
    14581 tcaatttggg atcaaccgca ggaacaattc cttatccagg agctaatgtc tatggcgcat
    14641 caaaagcttt tgtaaaacaa ttttctctca acttgcgtgc cgatttagct ggaactaaaa
    14701 tcagagttac taacctagaa cccggtttat gtgaagggac agaattttca actgtacgtt
    14761 tcaaaggaga tcataaaaga gttgaaaaac tttatgaagg tgcgcatgca attcaagcag
    14821 aggatattgc taataccgtg tcatgggttg ctagccaacc agaacacatt aatattaatc
    14881 gcatagaaat aatgccagtc agtcaaactt atggacctca acctgtttat cgttattaaa
    14941 atagtaaaga ccaggaaacg ctttaataat ttctggtctt tttatagata agtgttgttt
    15001 ttttaactta atctgctata atgagttcag ccattttttt gactaatatt atttaatcgg
    15061 agggttggca gaaatgcaac atgtcaatca ttcttctttt gataaagcat caaaagcagg
    15121 atttattatt gctttaggca ttgtttatgg agatattggt acaagcccac tctatacgat
    15181 gcaatcattg gttgaaaacc aaggtggtat ttctagtgtc acagaatcgt ttatcttagg
    15241 ttctatatct ttaatcatat ggaccttgac acttattaca actatcaagt atgtgcttgt
    15301 agctttaaag gcggataatc accacgaagg tggtattttt tctttatata cccttgttag
    15361 aaaaatgaca ccttggttaa ttgttccggc tgttattgga ggtgcaacct tgttgtcaga
    15421 tggagctttg acgccagctg taaccgtaac ttcagccgtt gaaggattaa aagtagttcc
    15481 tagtttgcag catattttcc agaatcagag taatgttatt tttgcgacct tgtttatttt
    15541 actgttactt tttgccatcc aaaggtttgg aacgggtgtt attgggaaat tatttgggcc
    15601 aattatgttt atatggtttg cctttttagg tattagcggt ctccttaata gttttgccca
    15661 tccagaagtt ttcaaagcaa ttaatccata ctacggtttg aaattgttat ttagtccaga
    15721 gaatcataaa ggtattttta ttttaggatc tattttcctg gcgacaacgg gggcagaagc
    15781 actatactct gacttaggtc atgttgggcg tggaaatata catgtttcat ggccgttcgt
    15841 taaggttgcc attatacttt cttattgtgg gcaaggggca tggattttag ctaataagaa
    15901 cgcaggaaat gaattgaatc ccttttttgc tagtattcct tcgcaattta caatgcatgt
    15961 cgttatttta gctactttgg cagctatcat cgcttcacag gcactgattt ctggatcatt
    16021 taccttagtt tctgaagcta tgcgactaaa aatattccca caatttcgtt caacttatcc
    16081 tggtgacaat attggtcaaa cctacatacc tgttattaat tggttcttat ttgccattac
    16141 aacctctatt gttttgcttt ttaagacttc agcgcacatg gaagcagcat atggattagc
    16201 gataacaatt acgatgctaa tgacaactat tttactgtct ttctttttaa ttcaaaaagg
    16261 agtaaaaaga ggtttagtct tattgatgat gattttcttt ggtattttag agggaatctt
    16321 cttccttgct tcagctgtta aatttatgca tggaggctat gttgttgtta ttatagcagt
    16381 agcaattata ttcatcatga ctatctggta taaaggtagt aaaattgttt cgcgttatgt
    16441 taagttgttg gatttaaaag attacatcgg acaattagat aaacttagac atgatcatcg
    16501 ttatccgatt tatcatacta acgttgttta tttgacaaat cgtatggagg gagacatgat
    16561 tgataagtct atcatgtatt ctatccttga taagcgccct aaaaaagcac aagtttattg
    16621 gtttgtaaac attaaggtca ctgatgaacc ttatacagct gagtataaag ttgacatgat
    16681 gggaaccgac ttcattgtga aggtagaact ttaccttgga tttaagatgc gtcagactgt
    16741 gtcacgctat cttcgtacga ttgtagaaga attacttgaa agtggacgtc tgcctaaaca
    16801 aggaaagaca tacagcgtta gacctgatag taaagtagga gattttaggt ttattgtcct
    16861 tgatgaacgt ttttcaagca gtcaaaacct aaaacctggt gaacgatttg ttatgctaat
    16921 gaaatcatct gtaaagcatt ggacagcaac accaatacga tggtttggtc tacaattttc
    16981 cgaagttaca acagaagtag ttcctttgat ttttacagct aatcgaggtc taccaattaa
    17041 agaaaaaatt gaacttacaa caactggaga ttgattaata aaaggcgctg gaattaaacc
    17101 agtgcctttt agttattagt tttcttttta gagtgacgag tggattatag ttcaaaatca
    17161 tcgttagaga tagaagcacc agacgtgaca tcagaccatc caacgatagc ttgaccggat
    17221 tcttttaaat acccctctaa tagtggttta atattatgga catgttcaat ataaccaact
    17281 agttgcccac tatcattttc taagcgttta taagattgta ctagaatata atcttgtgat
    17341 gcgccaggta agagtaaatg tagctctttc gcttttttct ctattagact gttttgaacc
    17401 catttgtcta taggatattc ttcttcagga taaatatcaa aaggatcttc aggtttatgg
    17461 ttcgaataaa ttgtttgacc gtccttatta tgcaatctaa aacgatatgg gagatggtta
    17521 aataagtcaa aatttagcat atagtgaaac tcggtcattg ttttggagag ttccgagaac
    17581 gttttaatat cgttagcctc tgtttttgag ttagtctgac taatcgctaa gtagttggag
    17641 tcaattacca gatgaggtat gctagtttct atatcatgga atgcaaagct accgtcaaaa
    17701 tctgcatgat tgagagttga aatatctgta atgatttgaa tagaaaacaa ataattaaag
    17761 taagtcttta acgtaataaa gaaattattt tcagattcat ttgaggcaaa agccactatt
    17821 ttcttgggta ttagcatatg aacatgataa cggtattaag agtaaattgc aattgttatg
    17881 ttctctgaag tggtgattga tgggtagaga atagataaga aagtgaaggc attaatattc
    17941 aaaaaactct ttgtttttct tagctttatt tgatataata agaactgttg attgtccttt
    18001 gacaactaat ataaacaatt taattttaat tctaatgaat taaatggagc accttgattt
    18061 agcggggtgc tctattttat gataataatc aaagtaagga gattttaatg actaaaaagg
    18121 gatggcgtat tatgcctgcc ataattacaa cggctatatt aggtttttca gggattctta
    18181 ttgaaacatc tatgaatgtc acttttccgc ttttgatgaa agaatttggg gttaaccccg
    18241 ctgttattca atgggtgaca acaggaaatt tgttagctgt tgctgtgact gtgcctttgt
    18301 cagcatttat gattaaaaat ttatcagaga gacaaatttt tacattggca aatgtgcttt
    18361 ttttatctgg tgttttaatt gatagctttg cccctaattt agctatattg ttagtaggga
    18421 gagtcttgca aggtgttggg acaggattag ccctcccttt attgtttcat atcattctta
    18481 cacaaatccc gatggagcgt agaggactaa tgatgggagt agctgctatg gtcacacttt
    18541 tagcaccggc agttggacct acttatggtg gtgtcatttc aggaatgtta ggatggaaga
    18601 tgatttttat gcttctggca ccaatactta tcatatctac ctttataggt ttggcttcta
    18661 ttcccaaacg tcaagtaaga attaatgata aactcaattt tcctgccttt gtcagtttag
    18721 gtattggctt ggcaaccctt cttttagcta ttgaaaagat gtctattttt tacttattag
    18781 tagctattgt tagttttgtt attttttact atttaaataa acagctagaa tttttgaatt
    18841 tgaatgtttt taaagataaa gatttctcaa tcttattgta tggcgtcctg gcttttcaaa
    18901 tgattcctct agcactttcg ttcttattac ctaacctctt gcaacttgtt ttacatcaaa
    18961 cttcaaccaa agctggtttg tttatgtttc caggtgcaat agcagtagtt tttttatccc
    19021 cttttgcagg ttatctcctg gataaaattg gtgcatttaa gccaattatg ataggtatct
    19081 ccctttcttt gataggttta attggtacag ctatattcat tcctgcgaag tttgttgtag
    19141 tacttttagc ctttgatatt cttactaaaa ttggtatggg gattggagca agtaatatgg
    19201 ttacgacagc tttaacaaaa ctaaagccag cacagtcagc ggatggtaat agtatcttaa
    19261 atacactaca acaatttgcg ggagcttttg caaccgcagt agcctcacaa atttttacca
    19321 tcggacaagt agctattccg aaaaatggag ctataatcgg tagtcaattt gcagttctat
    19381 tcgttatcat tgttgttatc ttagctattg taggattaac ttatcttcga aaaagaaaag
    19441 caatataata aatgtaaaat c
//
LOCUS       DATRNK010000038        19400 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.038, whole genome shotgun sequence.
ACCESSION   DATRNK010000038 DATRNK010000000
VERSION     DATRNK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19400
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.038"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..659
                     /locus_tag="VBC52_001308"
     CDS             <1..659
                     /locus_tag="VBC52_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO6882895.1"
                     /translation="HFENNILKAVKSGSEFLLKETVEQFSNSIVPIISGDELRSEKNY
                     SIMIYDRLSQATIQAGLDIETAYRARDRFIKENESTISLNEVLKLRDTAILFYTQQVH
                     SLKKHLETPHSQTIVAVIRYLENNLNRFIKTEEIAKECHMSESKLRKLFKQEKHITIQ
                     QYFLNLKIEAAKQLLDENKKVEEVSNLLGFSTSSNFSRTFKKIVGISPLEYKQKPKTI
                     "
     gene            complement(854..1378)
                     /locus_tag="VBC52_001309"
     CDS             complement(854..1378)
                     /locus_tag="VBC52_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO6882896.1"
                     /translation="MVKDRQIQKTKVAIYNAFISLLQENDYSKITVQDVIGLANVGRS
                     TFYSHYESKEVLLKELCEDLFHHLFKQGRDVTFEEYLVHILKHFEQNQDSIATLLLSD
                     DPYFLLRFRSELEHDVYPRLREEYITKVDIPEDFLKQFLLSSFIETLKWWLHQRQKMT
                     VEDLLKYYLTMVER"
     gene            1510..2373
                     /locus_tag="VBC52_001310"
     CDS             1510..2373
                     /locus_tag="VBC52_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO6882897.1"
                     /translation="MTSNKKVAIAFILNISFSVLEFIFGSLFFSGAILADAVHDFGDA
                     IAIGISATLEKKSKKDEDTIFSLGYKRFSLLGALITSLILISGSILVMIENIPKLWHP
                     TPVNYHGMFILAVIAIIINGLASFILHSGQSKHEEILSLHFLEDILGWLAIIVISLIL
                     NWKPLYILDPLLSVAISTFILSKALPKLLSTLKLFLDGVPDSIDYAALHDELKGLSQV
                     RSINQLNIWSMDGIDNRAIIHCCLNQLISEKDCKRAIRTICQHYKINDVTVEIDYSLR
                     EHQNHCKPLKN"
     gene            2458..3312
                     /locus_tag="VBC52_001311"
     CDS             2458..3312
                     /locus_tag="VBC52_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6882898.1"
                     /translation="MTDTMILNDNHKIPTLGLGTWMIDNDRVAQVVKDAIELGYRHID
                     TAQAYNNEKGGGVGIKESGITRESLFVTTKIAAEHKDYESATKSIDRSLEALGLDYID
                     LMIIHSPQPWKEWRETDKHFDQGNLEAWRALEDAQKSGKVKSIGVSNFLEKDLENILK
                     NGYVKPAVNQILAHIGNTPFDLIDYCQSKGIQVEAYSPIAHGQALKSDGIQKMAEKYG
                     VSVAQLCIQYLLQLNLIVLPKASSKEHLQSNLDFDFVISDEDMSILKSLMFDDYGEFS
                     NFPVFSGK"
     gene            3422..4449
                     /locus_tag="VBC52_001312"
                     /pseudo
     CDS             3422..4449
                     /locus_tag="VBC52_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861552.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            4465..4854
                     /locus_tag="VBC52_001313"
     CDS             4465..4854
                     /locus_tag="VBC52_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6882899.1"
                     /translation="MHIKEVSKKTGITSDTIRYYERINLIPPIHRSESGIREFTQNDI
                     DILEFVRYFRGAGVSVESLIDYIHLVEKGDSTIPARLEILKDKRNNLEDRLQGLQEAL
                     NRLNHKIDNYQKVLVPREQELFDPTTK"
     gene            4864..5121
                     /locus_tag="VBC52_001314"
     CDS             4864..5121
                     /locus_tag="VBC52_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEO6882900.1"
                     /translation="MQETTKSIFPKGEKNPYGEFFIRQSYLAALAKSPDGNVSVGNVT
                     FESGCRNNWHVHLDGYQILLVTEGSGWYQEENKKGSFIRTR"
     gene            5090..5260
                     /locus_tag="VBC52_001315"
     CDS             5090..5260
                     /locus_tag="VBC52_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEO6882901.1"
                     /translation="MKKAVSLEPGNVIVTDKGVKHWHGAKKDSEFAHIAITAGKSEFY
                     EAVSDEEYSRLG"
     gene            5288..5605
                     /locus_tag="VBC52_001316"
                     /pseudo
     CDS             5288..5605
                     /locus_tag="VBC52_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660666.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            5692..5853
                     /locus_tag="VBC52_001317"
     CDS             5692..5853
                     /locus_tag="VBC52_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HEO6882902.1"
                     /translation="MLTIQEIKDTTFELQENFRRLNYPIKQVAKDLQLNISEVESLLS
                     LDVTYPGDV"
     gene            5854..5976
                     /locus_tag="VBC52_001318"
     CDS             5854..5976
                     /locus_tag="VBC52_001318"
                     /inference="COORDINATES: protein motif:HMM:NF021585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HEO6882903.1"
                     /translation="MLRDYLEDMLKKEGKEVYPFSRLASHSANRWYPYETPWRY"
     gene            6215..6706
                     /locus_tag="VBC52_001319"
     CDS             6215..6706
                     /locus_tag="VBC52_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO6882904.1"
                     /translation="MLSFQIVEKPAMILAGVTLENVKSNQECPKLWEKLHKQYNPAKL
                     KEKGIQQAIGICKTQPDFRFDYSATYRVETSVQAPKGLEIIRIPSATYAVISVKGPMP
                     SSLQETWRKIIQGFFQENNLKPANSPNLEIYSSQHPQDTDYQMEIWLAIADITNKSKT
                     NLI"
     gene            complement(6750..7139)
                     /locus_tag="VBC52_001320"
     CDS             complement(6750..7139)
                     /locus_tag="VBC52_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEO6882905.1"
                     /translation="MITQEMKEIINSQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGAYYEAAKKWAEGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEIT"
     gene            complement(7152..10319)
                     /locus_tag="VBC52_001321"
     CDS             complement(7152..10319)
                     /locus_tag="VBC52_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEO6882906.1"
                     /translation="MTKKHLKTLALALTTVSVVTYSQEVYGLEREESVKQEQTQSASE
                     DDWFEEDNERKTNVSKENSTVDETVSDLFSDGNSNNSSSKTESVVNDPKQVLKAKPEV
                     TQEASNSSNNASKVEVSKQDTASKKEIIETSTWEAKDFVTRGDTLVGFSKSGINKLSQ
                     TSHLVLPSHAADGTQLTQVASFAFTPDKKTAIAEYTSRLGENGKPSRLDIDQKEIIDE
                     GEIFNAYQLTKLTIPNGYKSIGQDAFVDNKNIAEVNLPESLETISDYAFAHMSLKQVK
                     LPDNLKVIGELAFFDNQIGGKLYLPHHLIKLAERAFKSNRIQTVEFLGSKLKVIGEAS
                     FQDNNLSNVMLPDGLEKIESEAFTGNPGDEHYNNQVVLRTRTGQNPHQLATENTYVNP
                     DKSLWRATPDMDYTKWLEEDFTYQKNSITGFSNKGLQKVRRNKNLEIPKQHNGITITE
                     IGDNAFRNVDFQSKTLRKYDLEEIKLPSTIRKIGAFAFQSNNLKSFEASEDLEEIKEG
                     AFMNNRIGTLDLKDKLIKIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGALHLMFIG
                     NKVKTIGEMAFLSNKLESVNLSEQKQLKTIEVQAFSDNALSEVVLPPNLQTIREEAFK
                     RNHLKEVKGSSTLSQIAFNAFDQNDGDKRFNKKVVVRTHNNSHMLADGERFIIDPDKL
                     SSTMVDLEKVLKTLEGLDYSTLRQTTQTQFREMTTAGKALLSKSNLRQGEKQKFLQEA
                     QFFLDRVDLDKAIAKAEKALVTKKATKNGHLLERSINKAVLAYNNSAIKKANVKRLEK
                     ELDLLTDLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSD
                     TIGEGQKDAYGNPILNVDEDNEGYHALAVATLADYEGLYIKDILNSSLDKIKAIRQIP
                     LAKYHRLGFFQAIRNAAAEADRLIPKTPKGYLNTVPNYRKKQVEKNSKPVDYKTPIFD
                     KALPNEKVDGDRVAKGHNINAETNNYVAVTPIRSEQQLHKSQSDVNLPQTSSKNNFIY
                     KILGYVSLCLLFLVTSGKKGKRARK"
     gene            complement(10382..12550)
                     /gene="nrdE"
                     /locus_tag="VBC52_001322"
     CDS             complement(10382..12550)
                     /gene="nrdE"
                     /locus_tag="VBC52_001322"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO6882907.1"
                     /translation="MTQSDAYLSLNAKTRFRDRTGNYHFASDKEAVEQYMIEHVEPNT
                     MTFTSLIEKLDYLVSNNYYESDLLKQYNLEFICQIFEHAYAKKFAFLNFMGALKFYNA
                     YALKTEDNRYYLEHYEDRVVMNALFLAAGDEKAAYDLVDDMLANRFQPATPTFLNAGK
                     KRRGEYISCYLLRIEDNMESISRAISTSLQLSKRGGGVALCLTNLREFGAPIKGIKNQ
                     ATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKS
                     LSLGLVIPDITFELAKANKDMALFSPYDIERVYGKPMSDISITEEYETLLANADIRKT
                     FISARKLFQTIAELHFESGYPYILFEDTVNAKNPHKKEGRIVMSNLCSEIAQVNTASQ
                     FSEDLTFTKVGHDVCCNLGSINIARAMDQAADFEKLIANSIRALDRISRTSDLDSAPS
                     IKKGNAANHAVGLGAMNLHGFLATNHIYYDSQEAIDFTDCFFYAMAYYAFKASNHLAK
                     EKGTFEGFSESSYADGSYFYQYTEQNFEPKTQRVKNLLAEYGLTLPSQEDWRKLVQSI
                     KEIGLANAHLLAVAPTGSISYLSSCTPSLQPVVSPVEVRKEGALGRVYVPAYKIDADN
                     YVYYKKGAYEVGPEAIINIAAAAQKHIDQAISLTLFMTDQATTRDLNKAYIQAFKQKC
                     ASIYYVRVRQDILEGSESYDDDMLDDFTSSDLEDCQSCMI"
     gene            complement(12552..12965)
                     /gene="nrdI"
                     /locus_tag="VBC52_001323"
     CDS             complement(12552..12965)
                     /gene="nrdI"
                     /locus_tag="VBC52_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO6882908.1"
                     /translation="MDSTVQVVYFSSKSNNTHRFVQKLAWSNQRIPSDGSNILVTEDY
                     ILIVPTYAGGGDDTKGAVPKQVVQFLNVRQNREHCQGVISSGNTNFGDTYAIAGPIIA
                     RKLNVPLLHQFELLGTQEDVTRVKELLCQFTRKDK"
     gene            complement(12966..13976)
                     /gene="nrdF"
                     /locus_tag="VBC52_001324"
     CDS             complement(12966..13976)
                     /gene="nrdF"
                     /locus_tag="VBC52_001324"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO6882909.1"
                     /translation="MQSYYDRSQSPLDYALSEKAFPMRSVNWNKLNDDKDLEVWNRVT
                     QNFWLPEKIPVSNDLNSWRTLDADWQQLITRTFTGLTLLDSVQATVGDIAQIKHSQTD
                     HEQVIYANFAFMVAIHARSYGTIFSTLCTSQQIEEAHEWVVDTESLQARSRILIPFYT
                     GDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGY
                     KYQQKVAKLSVEKQAEMKTFVFDLLYQLIDLEKAYLYELYDGFDLAEDAIRFSIYNAG
                     KFLQNLGYDSPFTEEETRISPEVFAQLSARADENHDFFSGNGSSYIMGITEETLDEDW
                     EF"
     gene            complement(14528..15436)
                     /locus_tag="VBC52_001325"
     CDS             complement(14528..15436)
                     /locus_tag="VBC52_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6882910.1"
                     /translation="MKTIKDWQINICLATYNGQKYLRQQLDSIIQQGYTDWICLIRDD
                     GSTDDTVAIIKEYVNRDSRFIFINSNDDRKLGSHRSFYELVNYKKADFYVFSDQDDVW
                     KENRLERYLEEAEKFNQELPLLVYSNWTSVDEKLTVLKEHNPATVIQEQIAFNQINGM
                     VIMMNHELAKLWEYRQIGAHDSYVGTLAYAVGNVAYISDSTVLWRRQVGAESLNNYGR
                     QYGVATFWQMINTSFDRASLIFAQVSDKMSLERKLFFSRFIELKNANLMRRIYLLSKL
                     KLRRKSLKETVAMTILLLTGYGKPKA"
     gene            complement(15646..19347)
                     /locus_tag="VBC52_001326"
     CDS             complement(15646..19347)
                     /locus_tag="VBC52_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO6882911.1"
                     /translation="MDKHHSKKAILKLTLITTSILLMHSNQVNAEEQELKNQEQSPVI
                     ANVAQQPSPSVTTNTVEKTSVTAASASNTAKEMGDTSVKNDKTEDELLEELSKNLDTS
                     NLGADLEEEYPSKPETTNNKESNVVTNASTAIAQKVPSAYEEVKPESKSSLAVLDTSK
                     ITKLQAITQRGKGNVVAIIDTGFDINHDIFRLDSPKDDKHSFKTKTEFEELKAKHNIT
                     YGKWVNDKIVFAHNYANNTETVADIAAAMKDGYGSEAKNISHGTHVAGIFVGNSKRPA
                     INGLLLEGAAPNAQVLLMRIPDKIDSDKFGEAYAKAITDAVNLGAKTINMSIGKTADS
                     LIALNDKVKLALKLASEKGVAVVVAAGNEGAFGMDYSKPLSTNPDYGTVNSPAISEDT
                     LSVASYESLKTISEVVETTIEGKLVKLPIVTSKPFDKGKAYDVVYANYGAKKDFEGKD
                     FKGKIALIERGGGLDFMTKITHATNAGVVGIVIFNDQEKRGNFLIPYRELPVGIISKV
                     DGERIKNTSSQLTFNQSFEVVDSQGGNRMLEQSSWGVTAEGAIKPDVTASGFEIYSST
                     YNNQYQTMSGTSMASPHVAGLMTMLQSHLAEKYKGMNLDSKKLLELSKNILMSSATAL
                     YSEEDKAFYSPRQQGAGVVDAEKAIQAQYYITGNDGKAKINLKRMGDKFDITVTIHKL
                     VEGVKELYYQANVATEQVNKGKFALKPQALLDTNWQKVILRDKETQVRFTIDASQFSQ
                     KLKEQMANGYFLEGFVRFKEAKDSNQELMSIPFVGFNGDFANLQALETPIYKTLSKGS
                     FYYKPNDTTHKDQLEYNESAPFESNNYTALLTQSASWGYVDYVKNGGELELAPESPKR
                     IILGTFENKVEDKTIHLLERDAANNPYFAISPNKDGNRDEITPQATFLRNVKDISAQV
                     LDQNGNVIWQSKVLPSYRKNFHNNPKQSDGHYRMDALQWSGLDKDGKVVADGFYTYRL
                     RYTPVAEGANSQESDFKVQVSTKSPNLPSRAQFDETNRTLSLAMPKESSYVPTYRLQL
                     VLSHVVKDEEYGDETSYHYFHIDQEGKVTLPKTVKIGESEVAVDPKALTLVVEDKAGN
                     FATVKLSDLLNKAVVSEKENAIVISNSFKYFDNLKKEPMFISKKEKVVNKNLEEIILV
                     KPQTTVTTQSLSKEITKSGNEKVLTSTNNNSSRVAKIISPKHNGDSVNHTLPSTSDRA
                     TNGLFVGTLALLSSLLLYLKPKKTKNNSK"
BASE COUNT         6197 a         3554 c         3141 g         6508 t
ORIGIN      
        1 atcattttga aaataatatt ctaaaagctg ttaaatcagg tagtgaattt cttttgaaag
       61 aaacagtgga acaatttagc aattctatcg tacctataat cagtggagat gaattgcgat
      121 ctgaaaaaaa ctactccatc atgatttatg atcgtctctc acaggctacc atccaagcag
      181 gacttgatat cgaaacagct tatcgagcac gagatcgttt tataaaagaa aacgagtcaa
      241 ctataagtct aaatgaagtt ttaaaattac gtgatactgc tatcttattc tatactcaac
      301 aagttcattc tttaaaaaaa catctcgaaa cccctcattc tcaaactatt gtcgcagtga
      361 ttcgatatct tgaaaataat ctaaatcgct ttattaaaac agaagaaata gctaaagaat
      421 gtcacatgag tgaatcgaag ttacgaaaac tatttaagca agaaaaacac atcactattc
      481 aacaatattt tctaaactta aaaatcgaag ctgctaagca attactagat gaaaataaga
      541 aagtagaaga agtttccaat ttacttggat tttccacttc ttctaatttt tcaaggacat
      601 ttaaaaaaat agtgggaatt agcccactag aatataagca aaagcctaag acaatatagt
      661 aatcgaaaaa tattcatttt gcttatggtg acatcaacaa tcccaaagaa actctttcat
      721 caaggggact tttttatctc ccgaattata ctcatttaat ctaataactt caactcgcta
      781 tattagacaa tcaatccatt catcacctct gataacgcta atgaaataag agaatctccc
      841 aactctctat aatttacctc tctaccatag ttaaataata ttttagtaaa tcttcaacag
      901 tcattttctg tctttgatga agccaccact ttaaggtctc gataaaactg gataaaagaa
      961 attgttttaa gaaatcttca ggaatgtcca cctttgtaat atactcctca cgcaatctag
     1021 ggtaaacatc gtgttctaat tcacttctaa agcgtaataa aaaataggga tcgtcagata
     1081 ataataacgt agcgatacta tcttgatttt gttcaaaatg ttttaagata tgaactaaat
     1141 actcttcaaa cgtaacatct ctaccctgct taaataagtg gtgaaaaagg tcttcacaga
     1201 gctccttgag taacacttct ttgctttcat agtgagagta aaaagttgat cttcctacat
     1261 ttgcaagtcc gattacatct tgaaccgtta ttttagaata atcattttcc tgtagcaagc
     1321 ttataaaagc attataaatc gctacttttg ttttctgaat ttgtctatct tttaccatat
     1381 aggacacttt ctaagttttg ttcagtattg aacaagtgtt cttttttgtt tctagcaatt
     1441 ctcttttatc tcctagataa tagaagaaag aatcacacta caataccagt ataacaaaag
     1501 gagaaaaata tgacatcaaa caaaaaagtg gctatcgctt ttatattaaa tatcagcttt
     1561 tccgtccttg aattcatctt tggaagtcta ttcttctcag gagctatctt agccgatgct
     1621 gttcatgatt ttggtgatgc aatagctatt ggcatctcag ctactttaga aaaaaaatct
     1681 aagaaagatg aagatacgat attttcatta ggttataaac gttttagcct tttaggagct
     1741 cttatcacaa gtctaattct tataagtgga tctatcttag tcatgataga aaatattcca
     1801 aaattgtggc atcctactcc tgttaattat cacggcatgt ttattctagc tgtcatcgct
     1861 attatcatta atggtctagc tagttttatt ctccattctg gtcaaagtaa acatgaggaa
     1921 atattaagcc ttcatttttt ggaagatatt ctagggtggt tagctattat tgttatatct
     1981 cttatcttaa attggaaacc gttgtatatc cttgatccat tattatcagt tgccatatct
     2041 actttcatct tatctaaagc acttcctaaa ttactatcaa ccctgaaact ttttttagat
     2101 ggtgttccag attccataga ttacgctgca ttacatgatg aacttaaggg actttcgcaa
     2161 gtaagatcta tcaatcaact caatatctgg tcaatggatg gtattgataa tcgagcaatc
     2221 attcactgtt gcttaaatca attgatatct gaaaaagatt gtaaaagagc tattcgaacg
     2281 atttgccaac attataagat aaacgatgtt actgttgaaa tcgattattc tttaagagaa
     2341 catcaaaatc attgcaaacc attaaaaaac taacctgagt gttagttttt tccttgactt
     2401 ggagttaact ctaagtgtta agattaggtc aatactaatg aaagaaggaa aataaagatg
     2461 acagatacaa tgatattaaa tgacaatcat aaaataccaa ctttaggttt aggtacgtgg
     2521 atgatagata atgatagggt agctcaagtt gttaaagatg ctatcgaatt aggctaccgc
     2581 catattgata ctgcacaagc atacaacaat gaaaaagggg gcggtgtagg gattaaagaa
     2641 tctggtatta cacgtgaatc tttatttgtt acgactaaaa ttgccgctga acataaagat
     2701 tatgaaagtg ctacaaaatc aattgatcga tcactagaag ctttagggct tgattatatt
     2761 gatttaatga ttattcatag tccacagcct tggaaggaat ggcgtgaaac agataaacat
     2821 tttgaccaag gaaatttaga agcttggaga gctttagagg atgctcaaaa atctggtaag
     2881 gtaaaatcca tcggtgtctc aaacttttta gaaaaagact tagaaaatat cttaaaaaat
     2941 ggttatgtta aacctgctgt caatcaaatc ttagctcaca tcggaaatac acctttcgac
     3001 ttgattgatt actgtcaaag taaaggtatt caagttgaag cttattcacc aattgcacat
     3061 ggacaagcat tgaaatctga tggtattcaa aaaatggctg aaaaatatgg ggttagtgtt
     3121 gctcaattat gtattcaata tttactacaa ttaaacctga tcgttttacc aaaggcttct
     3181 agtaaagaac atttacagtc taatctagac tttgactttg taattagtga cgaagatatg
     3241 tcgatattaa aatcacttat gtttgacgat tatggggaat tttctaactt ccctgttttt
     3301 agtggaaaat gaaaccatat gcggattttt acagatgttt aattttcaaa atttttaagt
     3361 ttttcttgac ttgaagttaa ctctaagagt tataatgaag ttatagaaaa gaggtaaggt
     3421 tatgaaagta gctacattta ttgaacctgg caaaatggtt ataactgata caccaaaacc
     3481 agtcattgaa caagagacag atgctgttat caaaattgtt agagcctgtg tttgtggttc
     3541 agacttgtgg tggtatcgtg gtatttcaaa acgtgaaagt ggttcttttg caggtcatga
     3601 gactattggt atcgttgagg aagttggtac taaagtaact gacgtgtcaa aaggtgattt
     3661 tgttattgtt ccctttacac atggctgtgg ccaatgtccg tcttgtaagg ctggatttga
     3721 tggaaattgt acaaatcatc aagctgaaaa aaatgtagat accaaggtca atatctacgt
     3781 tatactaatg caaactgggc attggttaaa attcctggtc aaccttctga ctatgataat
     3841 gagacactta actctcttct caccttatca gatgtaatgg caactggtta tcatgctgca
     3901 gaagttaaag aaggtgatac ggtagttgtc atgggagatg gtgctgttgg actctgtggt
     3961 gttattgctg ctaaaatgtt aggtgctaac cgtatcattg caatgagtcg tcacaaagat
     4021 aggcaggaac tagcattgac ttttggtgca acggatattg tcgaagaacg gggtgacgaa
     4081 gccgttaaac gtgttttaga tttaaccaat caagcaggtg ccgatgctat tttagaatgc
     4141 gttggtacag agcagtctgt tgatacagct acccaaattg ctaggcctgg tgctgtcata
     4201 ggacgtgttg gtatcccaca aaatccagac atgaatacca ataatttatt ctggaaaaat
     4261 atcggcctta gaggtggcat tgcttctgta acaacatttg ataagtctgt ccttttagat
     4321 gctgttctaa ctcataaaat taacccaggc ttagttttta caaaatcctt tgtattagat
     4381 gatattcaaa aggcttatga agcaatggat aaacgtgacg ctatcaaatc cttagtgatt
     4441 gttgactgaa tatcgatgtt aaaaatgcat atcaaagaag ttagtaaaaa gactggcatt
     4501 acgtcagata ccattaggta ttatgagaga attaatctta ttccccctat tcatcggagt
     4561 gaatcaggaa ttcgtgagtt cacacaaaac gatattgata ttttagaatt tgtccgctat
     4621 tttagaggcg caggtgtttc cgttgaaagt ctcattgatt atattcattt ggttgaaaaa
     4681 ggcgattcca caatcccagc taggctagaa attttaaaag ataaacgcaa caaccttgaa
     4741 gataggcttc aaggcctcca agaagcactt aatcgtctaa atcacaaaat agacaattat
     4801 caaaaagttc ttgttcctag agaacaagaa ctttttgatc caacaacgaa atgaggaaat
     4861 cttatgcaag aaacaactaa gtctatcttt cctaagggtg aaaaaaatcc atatggtgaa
     4921 ttctttatta ggcaatccta tctcgcagca ttagcaaaat ctccagatgg gaatgtttca
     4981 gttggaaatg taacctttga atctggctgt cgtaataatt ggcatgttca tttggacggt
     5041 tatcaaatcc tcctcgttac agaaggttca ggttggtacc aagaagagaa taaaaaaggc
     5101 agtttcatta gaaccaggta acgtgattgt aactgataaa ggcgttaaac attggcatgg
     5161 tgctaaaaaa gattctgaat ttgcacatat tgcgattaca gctggtaaga gtgagtttta
     5221 tgaagctgtt agtgatgaag aatattcaag actaggttaa attcaagtaa ggaggaatca
     5281 atccattatg aaagaaaaac aaacagccgg acgtcgtcaa ttagaagaat ttgcacctga
     5341 atttgccaga tataatgatg atattttatt tggtgaggtt tgggcaaaag aggatcattt
     5401 aacagataaa acacgttcta ttattactat atctgcctta attagctgag gaaatcttga
     5461 acagttagag catcatcttc aatttgccaa acaaaacgga gttactaaag aagaaattgc
     5521 tgacattatc actcaccttg ctttttatgt tggatggcca aaagcttggt cagcctttaa
     5581 taaagctaag gaaatttgga tataaaataa tagacatagc aagggagata ctttcccaaa
     5641 agccaaaaag aaagatcaac caattattaa accaactatt aggagaatga tatgcttact
     5701 attcaagaaa taaaagatac aacctttgaa ttacaagaaa attttagaag gttaaattat
     5761 cctattaaac aagttgctaa agacttgcaa cttaatatca gtgaagttga atctctttta
     5821 agtcttgatg ttacttatcc tggagatgtt tagatgctga gagattatct agaagatatg
     5881 ttgaaaaaag agggtaaaga agtttatcca ttttctagat tagcatctca ttcagcaaat
     5941 cgttggtacc cttatgagac accatggcgt tattaaagac taaagattaa tccatcatct
     6001 ttagagaaat aaacaaaaat agttaataga ttctttttag aaaaaagcta taaaacaaga
     6061 gcctcttaga gaatactcta catcataaga tttcttcttt gaagcatagc attccaaaag
     6121 aaaattatga ttgctaacaa gacaattatt ctaaaaagaa actattttaa cgactctata
     6181 attacttaag gaatcataaa gaataaaggt ttttatgcta tcatttcaaa ttgttgaaaa
     6241 gccagcaatg atacttgctg gagtaacttt agaaaatgta aagtcaaatc aagaatgtcc
     6301 taaactttgg gaaaagttac ataaacaata taatccagca aaactaaaag aaaaaggtat
     6361 ccagcaagcc attggcattt gtaaaacaca acctgatttt cgtttcgatt acagcgccac
     6421 ttatcgagta gagacaagtg tccaagcccc aaaagggtta gaaattatta ggatcccaag
     6481 cgctacctat gcagtcattt ctgttaaagg tccaatgcca tcgagtcttc aagaaacatg
     6541 gcgaaaaata atacaaggat tttttcaaga gaataatcta aaaccagcta actcgccaaa
     6601 cttagaaatt tattcatctc aacatccaca agatactgat tatcaaatgg aaatttggct
     6661 tgctatcgca gatattacta ataaatcgaa aacaaactta atctagcctt gcttaatata
     6721 aaaaagcccc tttttgaggc ttttttatat taagtgattt ctttacctgc attagcacca
     6781 gattgtaaat taaaaatacg ctcgacgtga ataataccaa ctgctttcgg aacccccatg
     6841 cgtccttctg cccatttttt cgctgcttcg taataagcac cttctgtttg aatttcagca
     6901 gttcccacaa acctgtaacc taaaagacgt tcacgatcaa caaaggcaat ttcaattttc
     6961 ccattatctt caatgttaat acgagtttga ccatctgtat tttcattata aataaacgtt
     7021 ttatcatccc agagacgcat agaacgtttt ggaccaatat tgggttgacc tttagcatca
     7081 accgtcgcga ccattgctaa ctgactatta ataatctctt tcatttcttg tgtaatcata
     7141 ttaatttctc cttattttct tgctcgtttt cctttttttc cagaagttac taggaaaagc
     7201 aaacataaac taacgtatcc tagaatcttg tatataaaat tatttttaga acttgtttga
     7261 ggtaaattta catcagactg tgacttatgt aattgctgct cggaccttat tggtgttaca
     7321 gctacataat tattagtctc cgcatttata ttatgacctt tagccactct atcaccatct
     7381 accttttcat taggtaaagc cttatcaaaa atcggcgttt tataatcaac tggttttgaa
     7441 tttttctcca cttgtttttt acgataattt gggactgtat ttaggtaccc cttaggtgtc
     7501 ttaggaatca atcggtctgc ttctgccgct gcatttcgga tagcttggaa aaatcctaat
     7561 ctatgatatt ttgccaaagg aatctgacgt attgctttaa tcttatcaag ggaactattt
     7621 aaaatatctt tgatataaag accttcataa tcagctaaag tggcaactgc caaggcatga
     7681 taaccttcat tatcctcgtc aacatttaat ataggattac catatgcatc tttttgtccc
     7741 tcgccaatag tatcactcat atcaagtgca taaatcaatt ttccagactt gtcaaaataa
     7801 acgttcaatc cgatataata ttctggcaat ggtagaggcg tctttaataa ataaactcct
     7861 tgtaccattg tagcttgcgc taatggtcct tttccctcga ctaaatctgt cagcaagtct
     7921 aactcttttt ccaagcgctt aacattagct tttttaatag cactattatt ataagctaat
     7981 accgctttgt taatactcct ctcaagcaaa tgaccattct ttgttgcctt cttggtcact
     8041 aaagccttct cagctttagc tatggcttta tccaaatcaa cgcgatcaag gaaaaattgt
     8101 gcttcttgaa ggaatttttg tttttctcct tgtcggaggt tagattttga taacaacgct
     8161 ttacctgcag tagtcatttc tctaaactga gtttgagtag tctgacgtaa tgtagagtaa
     8221 tctaaacctt ctagtgtttt taaaaccttt tcaaggtcta ccattgtaga agatagctta
     8281 tctggatcaa tgataaaacg ctcaccatct gctaacatat gagaattatt atgtgtccta
     8341 acaaccactt tcttattaaa gcgtttgtcc ccatcatttt gatcaaaagc attaaaagca
     8401 atctgagata atgtagatga acccttcact tctttcaaat gattcctttt gaaagcctct
     8461 tcacgaatag tctgtaaatt tggcggtaag actacttcac taagggcatt atccgaaaaa
     8521 gcttggacct caattgtctt taattgtttt tgctcagaga gatttacact ttccagttta
     8581 ttggataaaa aagccatttc accaattgtt ttaaccttat ttccgataaa cataaggtga
     8641 agtgcaccat tttgtcgaaa agctgaacgt cctatttctt gtacagattc tggaagaaca
     8701 atggcgtaaa tatgattaat atggaaagca gcatcaccta ttttgataag tttgtctttc
     8761 aagtctagag ttccaatacg attattcata aaggctccct ctttaatctc ttctaaatct
     8821 tcacttgctt caaaggattt caagttatta gattgaaaag caaaagcacc tattttccga
     8881 atagttgagg ggagctttat ttcttccaaa tcatatttac gtaaagtttt actttgaaaa
     8941 tcaacattgc gaaaagcgtt atcaccaatt tcagtaatag taataccatt gtgttgtttt
     9001 ggaatttcta agtttttatt acgtcttacc ttttgtaagc ctttatttga aaaacctgta
     9061 atactatttt tttgataggt aaaatcttcc tctaaccatt tggtataatc catatcaggc
     9121 gttgcacgcc acaatgattt gtccggattg acgtaagtat tctcagtcgc aagttgatgt
     9181 ggattttggc ctgtccgtgt gcgcaatacg acctgattgt tgtaatgttc atctcctgga
     9241 tttcctgtaa aagcttctga ttctattttt tcaagtccat ccggaagcat aacattactt
     9301 agattattat cttgaaaact tgcttctcct ataaccttaa gcttacttcc caaaaactca
     9361 actgtttgaa tacgattaga tttgaaagcg cgttctgcta attttatcaa gtgatgtggc
     9421 aagtaaagct taccaccaat ctggttatca aaaaaagcta attctccaat gacctttagg
     9481 ttatctggta actttacttg ttttaaagac atgtgagcaa aagcatagtc tgaaatagtc
     9541 tcgagacttt caggaagatt aacctcagca atattcttat tgtccacaaa agcatcttga
     9601 ccaatagact tataaccatt tggaatagta agcttagtca actggtaagc attaaatatt
     9661 tctccctcat caataatttc cttctgatca atatctaaac gactcggttt cccattttct
     9721 cctagcctac ttgtatattc tgcaatggcc gtctttttat ctggagtaaa agcaaagcta
     9781 gctacttgtg tcaattgagt tccatctgct gcatgacttg gtaaaactaa gtgtgatgtt
     9841 tgagataact tattaattcc agattttgaa aaacctacta aagtatcccc tctagttacg
     9901 aaatcttttg cctcccaagt tgatgtttct atgatttcct tttttgaagc tgtatcctgt
     9961 tttgatactt ctactttgct agcattatta ctagaattgc ttgcttcttg tgtaacctct
    10021 ggttttgctt tgaggacttg tttagggtca tttactactg actcggtttt agaactagag
    10081 ttattactat ttccatcaga aaataaatca ctaactgttt catcaacagt agaattctct
    10141 ttagaaacat ttgttttcct ctcattatct tcttcgaacc aatcatcttc tgaagctgac
    10201 tgggtttgtt cttgtttgac cgattcctct ctttctaatc catatacctc ctggctgtat
    10261 gtcactactg atactgtagt aagtgccaag gcaagcgttt taagatgttt ttttgtcatt
    10321 acaataaagc ctttctaaaa gccaatatat ttctcttaat cttctgatcg agtatattta
    10381 gttaaatcat gcaggactga cagtcttcta agtccgatga agtgaaatca tccagcatat
    10441 catcatcata actctcgcta ccttctagga tgtcctgtct cactcgtaca taataaatag
    10501 aggcacattt ttgtttaaat gcttgaatat aggctttatt taaatctcgc gtagttgctt
    10561 gatctgtcat gaaaagcgtt agcgaaatag cttgatcaat gtgtttttga gcggcagctg
    10621 caatattgat aatcgcctca ggtcccactt cataagctcc ttttttgtag tagacataat
    10681 tatctgcatc aatcttataa gcaggtacat aaaccctccc cagtgctcct tccttgcgta
    10741 cttcgacagg tgatacaact ggttggaggc tcggagtaca agaagaaaga taactgattg
    10801 accctgtcgg agcaacagct aataaatgcg cattagctaa tcctatctct ttaatacttt
    10861 gcacaagctt tctccagtcc tcttggcttg gtagagtcag gccatactca gctagtagat
    10921 ttttaacacg ctgtgttttt ggctcaaagt tttgttcggt atattgatag aaataactgc
    10981 catcagcata acttgattcc gagaagcctt caaaggttcc tttttctttt gctaaatggt
    11041 tagaagcttt aaaggcataa taagccatag cgtaaaagaa gcaatctgta aaatcaatag
    11101 cttcttgaga atcgtaataa atatggttag tcgccaaaaa tccatgcaaa ttcatagcac
    11161 ctaaaccaac agcatgatta gctgcattac cttttttaat agatggagca ctatctaaat
    11221 cagaagttcg agatatacga tcaagggctc taatgctatt agcaattaat ttttcaaaat
    11281 ccgccgcttg atccatagca cgcgcaatat taatagaacc taaattacaa caaacatcat
    11341 gtccaacctt agtgaaagtc aggtcttctg aaaattgact ggctgtatta acttgggcaa
    11401 tctcagaaca taaattagac ataacaattc gtccctcttt tttgtgaggg tttttagcat
    11461 ttacagtatc ttcaaataag atataaggat aaccagattc aaaatgaagc tctgctatcg
    11521 tttgaaatag ttttcgtgca ctaataaagg ttttacgaat atccgcattg gctaataaag
    11581 tttcatattc ttctgtgata gagatatcag acattggttt accatatact cgctcaatgt
    11641 catacggtga aaacaaggcc atatctttat ttgccttagc caattcaaaa gtaatatcag
    11701 gaataacaag tcctagggaa agagatttaa ttcgaatttt ctcatcggca ttctcacgtt
    11761 tagtatctaa aaacgttaat acttcaggat ggtgagcgtg taagtaaact gcaccagctc
    11821 cttgtctttg tcctaattga ttggcgtaag aaaaagaatc ttctagtaat ttcatgacag
    11881 gtacaatacc agtagcttga tttttaatac cttttattgg agcaccaaat tcccgtaagt
    11941 tggttaagca aagtgctacc ccaccacctc gttttgataa ctgaaggctt gtactaatag
    12001 cacgagaaat agattccata ttgtcttcaa tgcgcaacaa atagcaagag atatattcac
    12061 cacgacgttt tttccctgca tttaaaaagg taggtgttgc tggttgaaag cgatttgcta
    12121 gcatgtcatc tactaggtca taggcagctt tttcatcacc agcagctaaa aacagagcat
    12181 tcattacaac acgatcttca tagtgttcca aataataacg attgtcttct gttttaagag
    12241 cataagcatt ataaaatttt aaagccccca taaaatttag aaaagcaaat ttcttagcgt
    12301 atgcatgctc aaaaatttgg caaataaact caaggttata ttgttttaga aggtccgatt
    12361 catagtagtt attagaaacc aaataatcta gcttttcaat tagtgatgtg aacgtcattg
    12421 tattaggttc aacatgttct atcatatatt gttcaacagc ctctttatcc gaagcaaaat
    12481 gataattacc tgtgcgatct ctaaagcgtg tcttcgcgtt caacgagaga tatgcatctg
    12541 attgtgtcat cttatttatc ctttctagtg aattggcaaa gtaactcctt tacccttgta
    12601 acatcttctt gtgttcccag taattcaaat tgatgaagca aaggaacatt taattttcga
    12661 gcaataatag gtccagcaat ggcataagtg tcaccaaaat tggtatttcc agaagaaata
    12721 accccttggc aatgttcccg attttgtcga acatttaaaa actgtacaac ttgttttgga
    12781 actgcgcctt tggtatcatc tcctcctcca gcgtaagtag gcacaatcaa aatataatcc
    12841 tcagtaacca atatattgct accatctgat ggaatacgct ggttagacca agccaacttt
    12901 tgaacaaaac gatgggtatt gttagatttc gatgaaaaat agacaacctg cactgtacta
    12961 tccatttaaa actcccaatc ttcatccaat gtttcttctg taatccccat aatatatgaa
    13021 gagccgtttc ctgagaagaa atcatgattt tcatctgcac gcgccgaaag ttgtgcaaaa
    13081 acctcaggag atatacgagt ctcttcttct gtaaatggag aatcatatcc taaattttgt
    13141 aaaaatttac cagcattgta aatgctaaac ctgattgcat cttcagccaa atcaaaacca
    13201 tcatatagtt cgtaaagata ggctttttct aaatcaatca actgataaag gaggtcaaag
    13261 acaaatgtct tcatttcggc ttgcttttct acagataatt tagcaacttt ctgttgatac
    13321 ttatagccac tgtaatagtt atgaattacc ttatctcgta aaattaaacg aataatatca
    13381 gacgtatttg gtaattttcc tctggctgaa aggtaaaaag gaaggtaaaa gccaccatat
    13441 aacaaaaatc ctggcatcat ggctgcagca accttagatt tcaaaggatc atcaccagta
    13501 taaaaaggaa taagtatacg tgatcgagct tgtagacttt cagtatctac tacccattca
    13561 tgtgcttcct caatttgttg gctagtgcat aaggttgaaa aaatcgttcc atacgatcgc
    13621 gcatgaatag ccaccataaa ggcaaagtta gcataaataa cttgttcatg atctgtctga
    13681 gaatgcttaa tctgagcaat atcaccaaca gtagcctgca cagaatctaa taaggttaga
    13741 ccagtaaaag tccgagtaat taattgctgc caatcagcat ccaatgttct ccaagaatta
    13801 aggtcattag aaactgggat cttttcaggt aaccagaaat tttgagttac tctgttccat
    13861 acctctaagt ctttgtcatc gtttagctta ttccaattaa cagaacgcat aggaaaagct
    13921 ttttctgaca aggcatagtc aagtggcgat tgagaacgat catagtatga ttgcattaaa
    13981 ctagtctcct aaatattaaa attaagcgag ttttaagcac caaaaaagcc tcccaagagg
    14041 actggatttt tgttaaaatc acacaactat tggaatgttc ttcttgatgt ttttttattc
    14101 tggtaagaag ctaactcttg cttcaaaacc actgttttgt taagttacgt taaacttttt
    14161 tgtcggttac ctttattaag gatctgctta aacagtatac taacacaaag ttttgtgtct
    14221 ttcaacatat atcaccacta tatcttgtgt tttcatcata atgtaaagaa tataaaaatt
    14281 aaaaaattaa gcaagcctaa tttttataaa aaaaggcttt accttcatac ttttcctgtg
    14341 taaaacaaaa aaggagtagc gattgctact ccttttccaa aacccgaaat taatgcgagc
    14401 caaggttcta ccttgttctt taatattatt atatactaat ttcgttaagt gtcaacaact
    14461 ttttgaaaaa taaatatttt ctatattttt aactacaact caacttgatt tccttcaacc
    14521 aaaaaaatta tgcttttggt ttaccgtaac ctgttaaaag taggatagtc atagcaactg
    14581 tttctttcaa tgatttccga cggagtttta attttgataa aagataaatc cttctcatta
    14641 gattggcatt ttttaattct ataaaacgac taaagaagag tttacgctcc agggacattt
    14701 tatcggatac ttgagcaaaa ataagcgaag cacgatcaaa agaagtattt atcatctgcc
    14761 aaaacgtagc caccccatat tgtctcccgt aattgtttag cgactctgcc cctacttgtc
    14821 tacgccaaag gactgtactg tcagaaatat aagccacatt tccaacggcg taagctagag
    14881 ttccaacata agaatcatgg gcgccaattt gacgatattc ccaaagcttt gctaactcat
    14941 gattcatcat gataaccata ccatttattt ggttaaaggc aatttgttct tgaataactg
    15001 ttgctggatt atgttctttc aatactgtta atttttcatc tacagaagtc cagttcgaat
    15061 aaacaagcaa tggtagttct tggttaaatt tctctgcttc ttcaaggtaa cgttctaaac
    15121 gattttcttt ccaaacatca tcctggtctg agaaaacata aaaatctgct tttttatagt
    15181 taaccaactc gtaaaaactg cggtgacttc ctaactttct gtcatcgtta gaattaataa
    15241 atataaaacg tgaatctcta ttaacatatt ctttaataat agctactgta tcatcagtag
    15301 atccatcgtc ccttattaaa caaatccagt cagtataacc ttgttgtatt atgctatcta
    15361 attgctggcg aaggtatttt tgaccattat aggtcgctaa gcaaatattt atttgccaat
    15421 cttttatcgt tttcattgct atcaaaactc cttaaggcta gttcatctta tgaaagctta
    15481 caaagtgaac ggtatcacat actaatcgta attataatat tataatatta tatgtcatta
    15541 atcaatataa aattaatcaa aaataagagt caatacccta aaagtccagc ttaacataat
    15601 gttaaactga acttttcatc atattgccga gtagtttact tagttttatt tactattatt
    15661 tttagtcttt ttgggtttca aataaagaag taaactagat aacaatgcca aagtaccaac
    15721 aaatagacca ttcgttgctc tatctgatgt actaggtaag gtatggttaa cagaatcccc
    15781 gttatgttta ggtgatatga tcttagctac tctgctacta ttattgtttg tagaagtgag
    15841 gactttctca tttcctgatt tagttatttc tttagacaat gattgagtag taactgtagt
    15901 ttgcggctta actaatatta tttcttctag attcttgttt actacttttt cttttttaga
    15961 aataaacata ggttcttttt tcaagttatc aaaatatttg aaactgttag aaattactat
    16021 agcgttttct ttctctgata ctactgcctt attcaagaga tcagacaatt ttaccgttgc
    16081 gaaattacca gctttatctt ccacaacaag tgtcaaggcc ttagggtcta ccgcaacctc
    16141 actctctcct atcttaaccg ttttaggaag tgtcacttta ccttcttgat ctatatggaa
    16201 ataatggtaa gaagtctcat ccccatattc ttcatctttt acaacatgag ataaaactaa
    16261 ttgtaaacga tatgtaggaa cataactact ttccttaggc atggctaagc ttaatgttcg
    16321 attagtttca tcaaactgag ctcgtgaagg aagatttggt gacttagtac ttacttgtac
    16381 tttaaagtct gactcctgac tatttgctcc ttctgctact ggtgtgtaac gtaagcgata
    16441 agtataaaaa ccatctgcta caactttgcc atccttatct aaaccactcc actgaagagc
    16501 atccatacga taatgaccat cactttgctt tggattatta tggaaatttt tacgataaga
    16561 tggtaaaacc ttactttgcc aaataacatt tccattttga tctagaactt gagcagaaat
    16621 atccttaaca tttcttaaga aagttgcctg gggagtgatt tcgtccctat ttccatcttt
    16681 atttggagaa atggcaaaat atggattatt cgctgcatct ctttccaaaa gatgaattgt
    16741 tttatcctca accttattct caaaagttcc taaaataatt ctttttggac tctccggtgc
    16801 taattctaac tccccaccat ttttgacata atcaacatag ccccaagacg ctgattgtgt
    16861 taacaaggca gtatagttgt tgctttcaaa aggagctgat tcattgtact ccaattggtc
    16921 tttatgagtt gtatcatttg gtttatagta gaaactacct ttagaaagcg tcttataaat
    16981 cggtgtttca agtgcttgta agttcgcaaa atcaccatta aatcctacaa aaggaatact
    17041 cattaactcc tgattactat ccttggcttc tttaaaacgt acaaaacctt ctaagaaata
    17101 accatttgcc atctgttctt ttaatttctg actaaattga ctagcatcaa tagtaaatcg
    17161 aacttgtgtt tctttatcac gaagaattac tttctgccaa ttagtatcta gcaaggcttg
    17221 tggtttaagg gcaaatttac ctttatttac ttgttctgtt gctacattag cttgataata
    17281 caattctttg acaccttcta caagtttatg aattgtaact gtgatatcaa atttatctcc
    17341 cattcgtttg agattaattt tagctttgcc atcgtttcca gtaatataat attgagcttg
    17401 gatagctttt tcagcatcaa ctacacctgc accttgctga cgtggtgaat aaaacgcctt
    17461 atcctcttca ctatataatg ctgttgctga gctcatgagg atgtttttag acaattctag
    17521 caatttttta gaatctaaat tcatcccttt atatttctca gccaaatgac tttgaagcat
    17581 tgtcattaat cctgcaacat gtggtgaagc catacttgta ccagacattg tttggtattg
    17641 attattatag gttgaagaat aaatttcaaa gccagaagct gttacatcag gcttgattgc
    17701 tccttcagct gtcacgcccc aacttgattg ttccagcata cgattaccac cttggctatc
    17761 aactacttca aaactctggt taaatgttaa ctgacttgaa gtatttttta tacgctcgcc
    17821 atctacttta ctaataatcc ccacaggtaa ttcacggtaa ggaattagaa aatttccacg
    17881 tttttcttga tcgttaaaaa taacgatacc aacaacacct gcatttgtag catgagtgat
    17941 tttagtcata aaatcaagtc caccaccacg ctcaattaat gcaatcttac ctttaaagtc
    18001 cttaccttca aagtcttttt ttgcaccata attggcataa accacatcgt aggccttacc
    18061 tttgtcaaaa ggtttagaag tcacaatcgg caacttaact aacttacctt caatagttgt
    18121 ttcaacgacc tcactgatag ttttaagtga ttcatagcta gcaacactca aagtatcttc
    18181 agaaatagct ggactattaa ccgtaccgta gtcaggatta gttgataatg gtttgctata
    18241 atccatacca aatgcgcctt catttccggc agccacaaca actgcaacgc ccttctcaga
    18301 agctaattta agtgctaatt taactttatc attgagagca attaaagaat cagctgtttt
    18361 tccaatactc atattaatcg tttttgctcc tagattaaca gcgtctgtga ttgctttagc
    18421 atatgcttca ccaaatttgt ccgaatcaat tttatctgga atacgcatta ataagacttg
    18481 agcatttggc gctgcacctt ctaaaagaag accattgatt gctggacgtt tactattacc
    18541 tacaaaaata ccagcaacgt gtgtaccatg cgaaatattc tttgcttctg aaccataacc
    18601 atctttcata gctgctgcaa tatcagccac cgtttctgta ttgttggcgt agttatgtgc
    18661 aaaaacaatc ttatcgttaa cccatttccc ataagtgata ttatgttttg cttttaattc
    18721 ctcaaattct gtcttagttt taaagctgtg cttatcatct tttgggctat ctaaacgaaa
    18781 aatatcatgg ttaatatcaa agccagtatc aataatagct actacatttc cctttcctct
    18841 ttgggttatg gcttgtaatt ttgttatttt agatgtatca agaacagcaa gcgatgactt
    18901 gctttctggc ttcacctctt catatgctga gggaactttc tgtgctattg cagttgaagc
    18961 atttgttact acattgcttt ctttattgtt ggttgtctct ggtttagagg gatattcttc
    19021 ttcaagatca gcccccaaat tagacgtatc aaggttttta gataactctt ctaataattc
    19081 atcttctgtt ttgtcatttt ttacagatgt atcacccatt tctttcgctg tattactagc
    19141 agaagcagct gttacagatg ttttttcaac agtattagta gttaccgatg gcgatggctg
    19201 ttgagcaaca ttagcaatta caggtgattg ctcttggttt tttaattctt gctcctctgc
    19261 attcacttga ttgctatgca ttaataaaat actagttgtt ataagtgtta actttaaaat
    19321 agcctttttt gagtgatgtt tatccacttt tcctatttcc tccaaaaatc agtatgcata
    19381 ctatattaca acataattaa
//
LOCUS       DATRNK010000039        19290 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.039, whole genome shotgun sequence.
ACCESSION   DATRNK010000039 DATRNK010000000
VERSION     DATRNK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19290
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.039"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..1397)
                     /locus_tag="VBC52_001327"
     CDS             complement(<1..1397)
                     /locus_tag="VBC52_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO6882912.1"
                     /translation="MSQKTTKVSQQEQMVKGTAWLTAGNFISRLLGAIYIIPWYAWMG
                     KHAAEANALFGMGYEIYALFLLISTVGIPVAVAKQVSKYNTLGKEEMSIYLVRKILQF
                     MLILGGVFALIMYIGSPLFASLSKGGQELVPILRSLTLAVLVFPSMSVLRGFFQGFNN
                     LKPYAISQVAEQIIRVIWMLLTAFYIMRLGSGDYIAAVTQSTFAAFVGMFASIAVLLY
                     FLWRYNMLSALIGKTPEHIKLDTKEILIETVKEAIPFIITGAAIQIFKLIDQFSFGNT
                     MALFTNYSSEELRVMFAYFSSNPGKVTMILIAVATAIAGVGIPLLTENFVKNDKKAAA
                     RLVVNNLQMLLMFLLPAVAGSVILAKPLYTVFYGLPQGQALGLFVISLIQTIILSIYT
                     VLAPMLQALFENRKAIIYFLYGLAAKVILQVPSIFLFHAYGPLFSTTVALCIPVILMY
                     LKIHEITGFKRQAIRR"
     gene            1521..2975
                     /locus_tag="VBC52_001328"
     CDS             1521..2975
                     /locus_tag="VBC52_001328"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEO6882913.1"
                     /translation="MITIDKILEILKNDHNFREILFHEHYYYNWTQNVTFNALSYDSR
                     QISSDTLFFAKGATFKKEYLDSAITAGLSFYVSEIDYGADIPVILVNDIKKAMSLISM
                     SFYNNPQNKLKLLAFTGTKGKTTAAYFAYHMLKVNHRPAMLSTMNTTLDGKSFFKSHL
                     TTPESLDLFRMMATAVENQMTHLVMEVSSQAYLTKRVYGLTFDVGVFLNISPDHIGPI
                     EHPTFEDYFFHKRLLMENSNAVVVNSQMDHFNIVKEQVEYIPHDFYGDYSENVITESQ
                     AFSFHVKGKLENTYDIKLIGKFNQENAIAAGLACLRLGVSIEDIKNGIAQTTVPGRME
                     VLTQTNGAKIFVDYAHNGDSLKKLLAVVEEHQKGDIILVLGAPGNKGQSRRKDFGDVI
                     NQHPNLQVILTADDPNFEDPLVISQEIASHINRPVTIIIDREEAIANASTLTNCKLDA
                     IIIAGKGADAYQIIKGNHDNYSGDLEVAKKYLKR"
     gene            3131..3925
                     /locus_tag="VBC52_001329"
     CDS             3131..3925
                     /locus_tag="VBC52_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6882914.1"
                     /translation="MSHIKAENIIVSYDQKEIINNLSLSILNQKITTIIGANGCGKST
                     LLKALTRIHKIKDGTITIDGHDIAHLPTKEIAKKIALLPQVLEATEGITVYELISYGR
                     FPHQKYLGNLTNDDRSKIHWAMEMTNVAQFANRDVDDLSGGQRQKVWIAMALAQDTDT
                     IFLDEPTTYLDMNHQLEVLELLKKLNNETQKTIIMVLHDLNLSARYSDYLVAMKTGKI
                     IYEGSPSQIMTKDIIKDIFKIDAHIIQDPISKQPVLLSYQLSTDQN"
     gene            3949..4881
                     /locus_tag="VBC52_001330"
     CDS             3949..4881
                     /locus_tag="VBC52_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6882915.1"
                     /translation="MKKIGIIVLTLLTFFLVSCGQQTKEESTKTTISKMPKIEGFTYY
                     GKIPENPKKVINFTYSYTGYLLKLGVNVSSYSLDLEKDSPVFGKQLKGAKKLTADDTE
                     AIAAQKPDLIMVFDQDPNINTLKKIAPTLVIKYGAQNYLDMMPALGKVFGKEKEANQW
                     VSQWKTKTLAAKKDLHHILKPNTTFTIMDFYDKNIYLYGNNFGRGGELIYDSLGYAAP
                     EKVKKDVFKKGWFTVSQEAIGDYVGDYALVNINKTTKKAASSLKESDVWKNLPAVKKG
                     HIIESNYDVFYFSDPLSLEAQLKSFTKAIKENTN"
     gene            4897..5922
                     /locus_tag="VBC52_001331"
     CDS             4897..5922
                     /locus_tag="VBC52_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO6882916.1"
                     /translation="MFKASKKLVQKNKSNHFWLVFFITLILFLIGCYASLRFGAINFK
                     TSDLITVLKNPLKNSNAQDVIFDIRLPRIIAAILVGAAMSQAGAIMQGVTRNAIADPG
                     LLGINAGAGLALVVAYAFLGSMHYSTILIVCLLGSVISCLLVFTLSYTKQKGYHQLRL
                     ILAGAMFSTLFTSVGQVVTLYFKLNRTVIGWQAGGLSQINWKMLIIIAPIIILGLLIS
                     QLLAHQLTILSLNESVAKALGQKTQLMTAFLLLIVLFLSASSVALIGTVSFIGLIIPH
                     FIKLFIPKDYRLLLPLIGFSGATFMIWVDLSSRIINPPSETPISSIISIVGLPCFLWL
                     IRKGKNL"
     gene            5919..6920
                     /locus_tag="VBC52_001332"
     CDS             5919..6920
                     /locus_tag="VBC52_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO6882917.1"
                     /translation="MIQKNKAPFVLISSVIILLLLILVSISLGYANTSVIDVLKLISG
                     KSDDAFLFIITNIRLPRIIVCIFGGASLGIAGLLLQTLTKNPLADSGILGINAGAGLV
                     IALTIGTFNVSNPTILYFLPLLAMFGGLVTIFLVYLMSYRRNHNISPTRLIVTGIGIS
                     TMISGIMISIISQSNNQKMDMIVEWLSGKITISSWTTIITFIPILILLWGLAYSHSRH
                     LNIMNLNEQTALALGLHLKKERIYTLVLASSLAAISVVLIGNITFIGLLAGHLSRRLL
                     GNNHKIILPSCLLIGAIILLVSDTIGRLLLVGTGIPTGLVVSIIGAPYFLWLMTKVD"
     gene            complement(6949..7602)
                     /locus_tag="VBC52_001333"
     CDS             complement(6949..7602)
                     /locus_tag="VBC52_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEO6882918.1"
                     /translation="MILTFNPGKLERQEFFKELINYLWIHDDVTLRQIKSHFTDYSKI
                     DRLLEEYINHGYILRQNKRYSLNLPFLSSLDGLALDDLVFIDSDSQIYQLLQKRKFVT
                     NLDNQTNHLVFVEETDFERNTLTLSNYFYKLTNGYPLSREQKKLYQLLGDVNSEYALK
                     YMSSFILKFLRKDSVKQKRTDIFIQALELLGYISLNQDTTYRLNAKLDVEALKIYLT"
     gene            complement(7662..8597)
                     /locus_tag="VBC52_001334"
     CDS             complement(7662..8597)
                     /locus_tag="VBC52_001334"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEO6882919.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAWGLDTEAVALGTPN
                     EETAYVLDYFGVQAPRVVESAKAEGVETVILTDHNEFQQSISDIKDVTVYGVVDHHRV
                     ANFETANPLYMRLEPVGSASSIVYRMFKENGVSVPKELAGLLLSGLISDTLLLKSPTT
                     HASDIPVAKELAELAGVNLEEYGLEMLKAGTNLSSKTAAELIDIDAKTFELNGEAVRV
                     AQVNTVDINDILARQEEIEVAIQEAIVTEGYSDFVLMITDIVNSNSEILALGSNMAKV
                     EAAFEFTLENNHAFLAGAVSRKKQVVPQLTESYNA"
     gene            complement(8714..9502)
                     /gene="pflA"
                     /locus_tag="VBC52_001335"
     CDS             complement(8714..9502)
                     /gene="pflA"
                     /locus_tag="VBC52_001335"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEO6882920.1"
                     /translation="MAEIDYKKVTGMIHSTESFGSVDGPGIRFIIFMQGCKMRCQYCH
                     NPDTWEMETNNSKERTVEDVLKEALRYKHFWGKDGGITVSGGEAMLQIDFITALFIEA
                     KKLGIHTTLDTCGFAYRATPEYHVILEKLLDVTDLVLLDLKEIDPEQHKIVTRQSNKN
                     ILQFARYLSDRGTPVWIRHVLVPGLTDIDDHLKRLGEFVQTLDNVDKFEVLPYHTMGE
                     FKWRELGIPYPLAGVKPPTPERVKNAKDIMKTESYTEYLKRIQN"
     gene            complement(9570..10904)
                     /locus_tag="VBC52_001336"
     CDS             complement(9570..10904)
                     /locus_tag="VBC52_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEO6882921.1"
                     /translation="MEDPGSQSLLLQFVILLILTLFNAFFSASEMALVSLNRSKVEQK
                     AEEGDKRYRRLLDVLENPNNFLSTIQVGITFISLLQGASLSASLGHVISGWLGNSATA
                     RTAGSIIALIFLTYVSIVLGELYPKRIAMNLKDRLAIVSAPIIIFLGKIVSPFVWLLS
                     ASTNLLSRITPMTFDDADEKMTRDEIEYMLTNSEETLDAEEIEMLQGIFSLDEMMARE
                     VMVPRTDAFMIDINNDAQSNIEGILSQNFSRVPVYDDDKDRVVGVLHTKRLLEAGFKT
                     GFDTIDLRKILQEPLFVPETIFVDDLLKALRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDTAEQFVREIDENIYIVLGTMTLNEFNDYFETELESDDVDTIAGYYLT
                     GVGSIPNQEEKVAYEVDSKDKHITLINDKVKDGRITKLKVLLSDIEQNIEKD"
     gene            complement(11084..11650)
                     /locus_tag="VBC52_001337"
     CDS             complement(11084..11650)
                     /locus_tag="VBC52_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000596040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6882922.1"
                     /translation="MIKRPIHLSHDFLAEVIDKEAITLDATMGNGNDTVFLAKSSKKV
                     YAFDIQEEAIAKTKAKLTEQGISNAELILDGHENLEQYVHTPLRAAIFNLGYLPSADK
                     TVITKPHTTIKAIKNVLDILEVGGRLSLMVYYGHDGGKSEKDAVIAFVEQLPQNNFAT
                     MLYQPLNQVNTPPFLIMVEKLQSYENEV"
     gene            complement(11643..12578)
                     /locus_tag="VBC52_001338"
     CDS             complement(11643..12578)
                     /locus_tag="VBC52_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747785.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEO6882923.1"
                     /translation="MKKRYRAINDYYRELFGEKIFKLPIDAGFDCPNRDGTVARGGCT
                     FCTVSGSGDAIVAPEAPIREQFYKEIDFMHRKWPEVNKYLVYFQNFTNTHAKLEIIKE
                     RYEQAINEPGVIGINIGTRPDCLPDETIYYLAELSERMHVTLELGLQTTYEVTSALIN
                     RAHSYDLYKKTVKRIRELAPKVEIVSHLINGLPGETHDMMVENVRRCVTDNDIQGIKL
                     HLLHLMTNTRMQRDYHEGRLRLLSQEDYISIICDQLEIIPKHIVIHRITGDAPRHMLI
                     GPMWSLNKWEVLNAIDKEMEKRQSYQGCKVEVKHD"
     gene            complement(12671..13312)
                     /locus_tag="VBC52_001339"
     CDS             complement(12671..13312)
                     /locus_tag="VBC52_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6882924.1"
                     /translation="MLSRQNSKLIQAFIAIILFFSLGLVIKYWPDTVISFDQTIQESV
                     RGQLPNLSTRFFKLITVIGNTVSQIAIAIMSVTFCYLKKWYPQARFIAVNAIISGICI
                     LSLKLIFQRVRPTLTHLVFAGGYSFPSGHSMGTFMIFGSIIILLQYYMPKSIWKLLYQ
                     GILGLLIFLIGLSRIYLGVHFPTDVLAGFILAYGILNLTAYIFLAKDWLKKVK"
     gene            complement(13293..13877)
                     /locus_tag="VBC52_001340"
     CDS             complement(13293..13877)
                     /locus_tag="VBC52_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6882925.1"
                     /translation="MTNTRKMVTIAILSALSFVLMMVSFPLIPGAEFLKVDFSILPML
                     VAFILFDLKSSYGVLLLRSLLKVILANRGPETFIGLPMNMVALALFLASFAIFWKNRE
                     SAKDFIKASLFGTVSLTVSMVALNYVFAIPLYAIFANFDIRTFIGVGNYLLTMVIPFN
                     IVEGILISIVFYLTYVACLPILERYKKTNVISSK"
     regulatory      complement(13946..14091)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(14178..15104)
                     /gene="pilC"
                     /locus_tag="VBC52_001341"
     CDS             complement(14178..15104)
                     /gene="pilC"
                     /locus_tag="VBC52_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2a pilus subunit PilC"
                     /protein_id="HEO6882926.1"
                     /translation="MKKIRKSLGLLLCCFLGLVQLAFFSVASVNADTPNQLTITQIGL
                     QPNTTEEGISYRLWTVTDNLKVDLLSQMTDSELNQKYKSILTSPTDTNGQTKIALPNG
                     SYFGRAYKADQSVSTIVPFYIELPDDKLSNQLQINPKRKVETGRLKLIKYTKEGKIKK
                     RLSGVIFVLYDNQNQPVRFKNGRFTTDQDGITSLVTDDKGEIEVEGLLPGKYIFREAK
                     ALTGYRISMKDAVVAVVANKTQEVEVENEKETPPPTNPKPSQPLFPQSFLPKTGMIIG
                     GGLTILGCIILGILFIFLRKTKNSKSERNDTV"
     gene            complement(15101..15985)
                     /gene="srtC4"
                     /locus_tag="VBC52_001342"
     CDS             complement(15101..15985)
                     /gene="srtC4"
                     /locus_tag="VBC52_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2a pilus assembly sortase SrtC4"
                     /protein_id="HEO6882927.1"
                     /translation="MGGKFQKNLKKSVVLNRWMNVGLILLFLVGLLITSYPFISNWYY
                     NIKANNQVTNFDNQTQKLNTKEINRRFELAKAYNRTLDPSRLSDPYTEKEKKGIAEYA
                     HMLEIAEMIGYIDIPSIKQKLPIYAGTTSSVLEKGAGHLEGTSLPIGGKSSHTVITAH
                     RGLPKAKLFTDLDKLKKGKIFYIHNIKEVLAYKVDQISVVKPDNFSKLLVVKGKDYAT
                     LLTCTPYSINSHRLLVRGHRIKYVPPVKEKNYLMKELQTHYKLYFLLSILVILILVAL
                     LLYLKRKFKERKRKGNQK"
     gene            complement(15985..16863)
                     /locus_tag="VBC52_001343"
     CDS             complement(15985..16863)
                     /locus_tag="VBC52_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEO6882928.1"
                     /translation="MKTKNINKKTKKKKSNLPFIILFLIGLSILLYPVVSRFYYTIES
                     NNQTQDFERAAKKLSQKEINRRMTLAQAYNDSLNNVHLEDPYEKKRIQKGVAEYARML
                     EVSEKIGTISVPKIGQKLPIFAGSSQKVLSKGAGHLEGTSLPIGGNSTHTVITAHSGI
                     PDKELFSNLKKLKKGDKFYIQNIKETIAYQVDQIKVVTPDNFSDLLVVPGHDYATLLT
                     CTPIMVNTHRLLVRGHRIPYKGLIDEKLMKEGHLNTIYRYLFYISLVIIAWLLWLIKR
                     QRQKNRLASVRKGIES"
     gene            complement(16975..18999)
                     /gene="pilB"
                     /locus_tag="VBC52_001344"
     CDS             complement(16975..18999)
                     /gene="pilB"
                     /locus_tag="VBC52_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2a pilus major subunit PilB"
                     /protein_id="HEO6882929.1"
                     /translation="MKKINKCLTMFSTLLLILTSLFSVAPAFADDATTDTVTLHKIVM
                     PQAAFDNFTEGTKGKNDSDYVGKQINDLKSYFGSTDAKEIKGAFFVFKNETGTKFITE
                     NGKEVDTLEAKDAEGGAVLSGLTKDNGFVFNTAKLKGIYQIVELKEKSNYDNNGSILA
                     DSKAVPVKITLPLVNNQGVVKDAHIYPKNTETKPQVDKNFADKDLDYTDNRKDKGVVS
                     ATVGDKKEYIVGTKILKGSDYKKLVWTDSMTKGLTFNNNVKVTLDGEDFPVLNYKLVT
                     DDQGFRLALNATGLAAVAAAAKDKDVEIKITYSATVNGSTTVEIPETNDVKLDYGNNP
                     TEESEPQEGTPANQEIKVIKDWAVDGTITDANVAVKAIFTLQEKQTDGTWVNVASHEA
                     TKPSRFEHTFTGLDNAKTYRVVERVSGYTPEYVSFKNGVVTIKNNKNSNDPTPINPSE
                     PKVVTYGRKFVKTNQANTERLAGATFLVKKEGKYLARKAGAATAEAKAAVKTAKLALD
                     EAVKAYNDLTKEKQEGQEGKTALATVDQKQKAYNDAFVKANYSYEWVADKKADNVVKL
                     ISNAGGQFEITGLDKGTYGLEETQAPAGYATLSGDVNFEVTATSYSKGATTDIAYDKG
                     SVKKDAQQVQNKKVTIPQTGGIGTILFTIIGLSIMLGAVVIMKKRQSEEA"
     gene            complement(19122..>19290)
                     /locus_tag="VBC52_001345"
     CDS             complement(19122..>19290)
                     /locus_tag="VBC52_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6882930.1"
                     /translation="LITNTHIPPKGIIPMTGGKGILSFILIGGSMMSIAGGIYIWKRY
                     KKSSDISREKD"
BASE COUNT         6206 a         3588 c         3069 g         6427 t
ORIGIN      
        1 cgacgaatag cttggcgttt aaatcctgta atttcatgta ttttcaaata cataagaatt
       61 actgggatac aaagagctac tgtcgttgag aaaagtggtc cataggcatg gaaaaggaaa
      121 atggagggaa cttgtaaaat aacttttgcc gccagaccat ataggaagta aataatagct
      181 ttacgatttt caaacaatgc ttgtagcatt ggtgctaaaa cagtataaat agataaaata
      241 attgtttgaa taagagatat gacaaaaagc cctaaagctt gtccctgcgg taacccataa
      301 aagactgtat acaaaggttt tgctaaaata acggatcctg caacagctgg cagtaaaaac
      361 attagtaaca tttgtaaatt attaacaacc aaacgagcgg cggctttttt atcattctta
      421 acaaaattct ctgttaacaa tggaatacca actccagcaa ttgcggtagc aacagcaatt
      481 agaatcatcg taactttacc agggttgctt gaaaagtaag caaacataac ccgtaattct
      541 tcacttgaat aattcgtaaa gagcgccatc gtattcccaa atgaaaactg atcaatcaat
      601 ttgaaaattt gaattgctgc accagtaata ataaaaggga ttgcttcctt aacagtctca
      661 attagtattt ctttagtatc taatttaatg tgctctggag ttttaccgat caaggcagat
      721 agcatattat aacgccataa gaaataaagc agcacagcta tactagcaaa cataccgaca
      781 aaggcggcga atgttgactg tgtcacagcg gcaatataat ctcctgatcc tagacgcata
      841 atatagaaag cagttaataa catccatatg acacgtataa tctgctcagc gacttgacta
      901 atggcatacg gttttaaatt attaaaacct tgaaaaaaac cacgtaaaac actcattgaa
      961 ggaaaaacta aaacagctag agttagacta cgcaatattg gaacgagttc ttgtcctcct
     1021 ttactaagtg aagcaaatag aggtgaacca atatacataa ttaatgcaaa aactccacct
     1081 aaaatcagca taaactgcaa gattttccga accaggtaaa tgctcatctc ttctttacct
     1141 aaagtgttat acttagatac ctgttttgca actgctactg gaattccaac agttgatatc
     1201 aaaagaaata aagcatagat ttcatatccc atcccaaata aagcatttgc ttcggcagca
     1261 tgctttccca tccaagcata ccaagggata atatagatgg ctcccagcaa gcgactaatg
     1321 aaattccctg ctgttaacca agccgtccct ttgaccattt gttcttgctg actaactttt
     1381 gttgtttttt gtgacatggt aaactttcta ttttcaatat tatttagcat ttaaaagaca
     1441 aaaatctcag aaatatcact ttattataaa cttttacctt agacttgtaa aacgagaaca
     1501 taaagtctaa aataaggagt atgattacaa tagataaaat tttagaaatt ttaaaaaatg
     1561 accataattt cagagagatt ttgtttcatg agcattatta ttataactgg acacaaaatg
     1621 taacttttaa tgccttatca tatgatagtc gtcaaatttc atctgatact ttatttttcg
     1681 ctaagggtgc tacttttaaa aaagaatatc tagattctgc aatcacagca ggcctttctt
     1741 tttacgtttc tgagatagat tacggcgccg atattccagt gatattagtc aatgatatca
     1801 aaaaagccat gagtttgata tctatgtctt tctataataa cccacaaaac aaacttaaac
     1861 ttctagcttt tactggaaca aaaggaaaaa caactgcggc ttactttgct tatcatatgt
     1921 taaaagtaaa ccatcgtcct gctatgctct caactatgaa cacaacatta gatggtaaat
     1981 ctttctttaa atcacatctc acaacccccg agagtcttga tttatttcgg atgatggcta
     2041 cagctgttga gaatcaaatg acacacttag ttatggaagt atcaagccaa gcatacctaa
     2101 ccaaacgtgt ttatggttta acctttgacg ttggcgtttt cttaaatatt agtcccgatc
     2161 atattggtcc tatcgagcac cctacattcg aagattattt tttccacaaa cggctcttga
     2221 tggagaatag caatgctgtt gttgtaaata gtcagatgga tcattttaat attgtcaaag
     2281 aacaggtaga atatattcct catgatttct atggagatta ttctgaaaat gttatcacag
     2341 aatcacaggc cttttctttc catgtcaaag gaaagttgga gaacacctac gatatcaagc
     2401 tgattggaaa attcaatcaa gaaaatgcta tcgcagctgg acttgcttgt ctccgacttg
     2461 gtgtttcaat tgaagatata aaaaacggaa ttgcacaaac taccgttcct ggccgcatgg
     2521 aagtcctaac acaaaccaac ggcgcaaaga ttttcgttga ctatgcccac aacggagaca
     2581 gtttgaagaa attattagct gttgttgagg aacaccaaaa aggggatatt atattagtgc
     2641 tcggagctcc tggtaataaa ggtcaaagcc gtcgtaaaga cttcggagat gtcattaatc
     2701 aacatcctaa tctacaagtg atactcactg cagatgatcc taattttgaa gatcccttag
     2761 ttatctcaca agaaatagca agccatatta atcgtccggt tactatcatc attgatcgag
     2821 aagaagcaat tgctaatgcc agtactctta ctaattgcaa actagatgca attattatcg
     2881 ctggtaaagg ggcagatgct tatcaaatta tcaaaggaaa tcatgacaat tactctggag
     2941 atttagaagt tgcgaaaaaa taccttaaac gataaaaaaa gctaaacaat aatgattatt
     3001 gatactattt tgaatcattc taaataatta tcattttgtt cacatttttc ttaattaatg
     3061 ctaaaaaagg cttttgtaag ccttttttct atgttataat gaacttaatt ttttgctaga
     3121 aaggttctct atgtctcaca taaaagcaga aaacattatt gtatcttacg atcagaaaga
     3181 aatcatcaat aatctttccc tcagtatact taaccaaaaa ataacaacga ttattggtgc
     3241 caatggatgt ggaaagtcta ctcttttgaa ggctctcaca cgcatccata aaataaaaga
     3301 tggtactatc actattgatg gtcatgatat tgcacattta cccacaaagg aaattgctaa
     3361 gaagattgca cttctcccac aggtgttaga agcaactgaa gggataacag tttatgaact
     3421 aatatcttat ggacggttcc cacaccaaaa ataccttggc aatttgacaa atgacgatcg
     3481 tagtaaaatc cattgggcga tggaaatgac taacgtagcg caatttgcta atagagatgt
     3541 tgatgacctt tcaggaggtc agaggcaaaa agtatggatt gctatggctt tagcacaaga
     3601 tacagatact atctttttag acgaacctac aacttatctt gacatgaatc atcaattaga
     3661 agtcttagaa ttgctcaaaa aacttaataa cgaaacgcaa aaaacaatta ttatggtcct
     3721 acacgaccta aacctgtctg cgcgctattc tgattatcta gttgcaatga aaaccggtaa
     3781 aattatctat gaaggtagtc ctagtcagat catgactaaa gatattatca aagatatttt
     3841 taagattgat gctcatatca ttcaggaccc tattagtaaa caacctgttt tgttatctta
     3901 tcaattatca actgatcaaa attaatatta tttaggagat gttatcttat gaaaaaaatt
     3961 ggaattattg tcctcacact actgaccttc tttttggtat cttgcggaca acaaactaaa
     4021 gaagaaagca ctaaaacaac tatttctaaa atgcctaaaa ttgaaggctt cacctattat
     4081 ggaaaaattc ctgaaaatcc gaaaaaagta attaatttta catattctta cactggatat
     4141 ttattaaaac taggagttaa tgtttcaagt tacagtttag acttagaaaa agatagcccc
     4201 gtttttggta agcaactgaa aggagctaaa aaattaactg ctgatgatac agaagctatt
     4261 gccgcacaaa aacctgattt aatcatggtt tttgatcaag atccaaacat caatactctg
     4321 aaaaaaattg caccaacttt agttattaaa tatggtgcac aaaattattt agatatgatg
     4381 ccagctttgg ggaaagtatt cggtaaagaa aaagaagcta atcagtgggt tagccaatgg
     4441 aaaactaaaa ctctcgctgc caaaaaagat ttacaccata tcttaaaacc taacactact
     4501 tttaccatta tggattttta tgataaaaat atctatttat atggtaataa ttttggacgc
     4561 ggtggagaac taatctatga ttcactaggt tatgctgccc cagaaaaagt caaaaaagat
     4621 gtctttaaaa aagggtggtt taccgtttcg caagaagcaa tcggtgatta cgttggagat
     4681 tatgcccttg ttaatataaa caaaacgact aaaaaagcag cttcatcact taaagaaagt
     4741 gatgtctgga agaatttacc agctgtcaaa aaagggcaca tcatagaaag taactacgac
     4801 gtgttttatt tctctgaccc tctatcttta gaagctcaat taaaatcatt tacaaaggct
     4861 atcaaagaaa atacaaatta aaagaaagct cctcttatgt ttaaagcttc aaaaaaactc
     4921 gttcaaaaaa ataagtcaaa tcatttttgg cttgtttttt tcataacact tatactattt
     4981 cttattggtt gttacgctag cttacgattt ggtgctatca atttcaagac tagtgattta
     5041 ataaccgttt taaaaaatcc cttaaaaaac tctaatgctc aggatgttat ttttgacatt
     5101 agactaccac gcataattgc agctatctta gtaggtgcag ctatgtcaca agcaggtgct
     5161 attatgcagg gagtcactcg aaacgccatt gctgatcctg gactactagg tattaacgcc
     5221 ggagctgggt tagcccttgt tgttgcatat gcttttttag ggagcatgca ctatagtacg
     5281 attctcatag tctgcttact agggtctgtc atttcttgtc ttttagtctt cacactctct
     5341 tatacaaagc aaaagggtta ccaccaacta cgactcattt tagccggagc gatgttctcc
     5401 acacttttta cgtctgtcgg acaagtggtt acattatact ttaaactcaa tcgaactgtt
     5461 atcggttggc aagcaggtgg tttatcccaa attaactgga aaatgctaat cataatagca
     5521 cctatcatta ttttaggttt acttatttca caactcctag cacatcaatt aactatcttg
     5581 agccttaatg aatctgtggc taaagcatta ggacaaaaaa ctcaattaat gactgcgttt
     5641 ctattactta ttgttttgtt tttatcggct tcatcagtcg ctttgatagg aaccgtttct
     5701 ttcattggat tgattattcc acattttatt aagcttttta ttcccaaaga ttatcgtctg
     5761 ttattgcctt taataggatt ttcaggggct actttcatga tctgggtaga cttatcatct
     5821 cgtattataa atcccccttc agagacacca attagttcta taatcagtat cgtagggctt
     5881 ccatgtttcc tatggttaat tagaaaaggg aaaaatctat gattcaaaaa aataaagctc
     5941 cttttgtttt aatatcatct gtcattatcc tactattatt aatactagta tcaatttcac
     6001 ttggctatgc taatacttcc gttattgacg ttttaaaact aataagtgga aaatctgatg
     6061 atgccttcct ttttatcatt actaacattc gtttacctcg tattattgtc tgtatctttg
     6121 ggggagcttc tttaggaatt gctgggttgc tattacagac attaaccaaa aatccactag
     6181 ctgattccgg tattcttggt atcaatgctg gagctggatt agtaatcgct ctaaccatag
     6241 gaacatttaa tgttagtaat ccgactatct tatacttttt acccctactt gctatgtttg
     6301 gagggttggt aactattttt cttgtctatc ttatgtcata cagaagaaat cacaatatta
     6361 gccctacacg attaatcgtc actggtatcg gtatatctac aatgatttca ggcattatga
     6421 tttctattat tagtcagtct aataatcaaa aaatggatat gatcgttgaa tggcttagtg
     6481 gaaaaattac aattagttcc tggactacca ttatcacttt tatcccgatt ctcattttgt
     6541 tatggggatt agcatatagt cacagtcgcc accttaatat tatgaactta aacgagcaga
     6601 ctgctctcgc tctaggactt catttaaaaa aggaaagaat atatactttg gtgctggcaa
     6661 gtagccttgc tgctattagc gtcgtcttaa ttggtaatat tacttttata ggcttactcg
     6721 ctgggcactt aagtagacga ctattaggaa ataaccataa aattatacta cctagctgtc
     6781 ttcttattgg tgctattatc ctattggtat cagatactat aggtcgtctc ctcttagttg
     6841 gtacaggtat tcccactgga ttggttgtat caataattgg tgccccctat tttctctggt
     6901 tgatgacaaa agttgactaa aaaagactgg acaaacttcc agtctttttt atgttaagta
     6961 aatctttaaa gcttcaacat caagtttagc atttaaacga taggtagtat cttggtttaa
     7021 gctaatatat ccaagtaatt ctaaagcttg gataaaaata tctgtacgtt tttgcttaac
     7081 actatccttg cgaagaaatt tcaatataaa gcttgacata tattttaaag catattccga
     7141 attaacatct cctaaaagtt gataaagctt tttttgttct cttgaaagtg gataaccgtt
     7201 agttagttta tagaaataat tagaaagagt caaagtgttt ctttcaaaat ctgtctcttc
     7261 tacaaatacc aagtgattgg tttggttatc taaatttgtc acaaattttc gcttctgtaa
     7321 taactgatag atttgactat cagaatctat gaaaaccaaa tcatctaatg ctaatccatc
     7381 cagcgaactc agaaaaggaa ggttaagact gtagcgttta ttttggcgaa ggatatatcc
     7441 atgattaata tattcttcta acaaacggtc aattttagaa tagtctgtaa agtgggactt
     7501 tatttgtctt agtgttacat catcatgtat ccaaagatag ttaataagct ccttaaaaaa
     7561 ctcttgtctt tctaactttc ctggattaaa cgttaatatc ataaaagcgc tcctaaatgt
     7621 atgactaaaa aaacgggacg cagcccgttt tacatgatta attaagcatt gtagctttct
     7681 gtcaattgag gaacaacttg ttttttgcgt gatactgccc cagcaagaaa agcgtggtta
     7741 ttttcaagtg taaattcaaa agctgcttca actttagcca tatttgaacc gagagctaaa
     7801 atctctgaat ttgaattaac aatgtcagta atcatcaata caaaatcaga ataaccttct
     7861 gtgacaattg cttcttgaat agcaacttca atctcttctt gacgtgctaa aatatcgtta
     7921 atgtccacag tattaacttg tgcaacacga acagcttctc catttaattc aaatgtttta
     7981 gcatcaatat caatcaactc cgctgcagtc ttacttgaga ggttagtacc tgctttaagc
     8041 atttctaaac catattcttc taagtttaca cctgcaagct ctgctagttc ttttgcaact
     8101 ggaatatctg aagcatgagt tgttggtgat ttcaaaagaa gagtatctga aatcaaacct
     8161 gataataata aaccagccaa ctctttgggt actgatactc cattttcctt aaacatacgg
     8221 taaacaatcg atgatgctga gccaactggt tccaaacgca tatataacgg attggcagtc
     8281 tcaaagttag caacacgatg atgatcaaca acaccataaa ctgtcacatc tttaatatca
     8341 gaaatagatt gttggaattc gttatggtca gttaaaatga ctgtctctac accctctgct
     8401 tttgcagatt ctactactcg aggtgcttga acaccaaaat aatctaaaac atatgctgtt
     8461 tcttcatttg gagttcctaa agcaacggcc tctgtgtcaa gcccccaagc ttctttagct
     8521 agatatgcaa aagcaactga tgatccaata gcatctgaat ctggattttg gtgaccaaag
     8581 actaaaattt tagacatgtt tttacctctt cttttatatt caccttattg tagcaaaaat
     8641 tgaataactt agcaaaaaat cttactagga agccctttgc aactaaaaaa gttgaataaa
     8701 attcaactta aaactaattt tgaattcttt ttagatattc tgtataggat tctgttttca
     8761 tgatatcttt agcgttttta acacgttctg gagttggtgg tttcacacca gctaatggat
     8821 acggaattcc taattcacgc catttgaact ctcccatggt atgataaggc aatacttcaa
     8881 atttatcgac attatcaagt gtttggacaa attcacccaa acgttttaaa tggtcatcga
     8941 tatcagtcaa tccgggaact aaaacatgac gaatccatac cggagtacct ctatcagata
     9001 aatatctagc aaactgtaaa atatttttgt tagactgacg agttacaatt ttatgttgtt
     9061 ctggatcaat ttcttttaag tcaaggagta ccaaatcagt aacatctaac aatttttcaa
     9121 ggataacatg gtattctggt gttgctcgat aagcaaaacc acaagtatct agcgtcgtat
     9181 gaatacctag tttcttagcc tcaataaaga gtgctgtaat aaagtcaatt tgcagcattg
     9241 cttcaccacc tgaaactgtg atacctccat cttttcccca aaaatgttta taacggagag
     9301 cttcctttag aacatcctca acggtacgtt cctttgaatt gttagtttcc atttcccaag
     9361 tatcaggatt atgacagtat tgacaacgca ttttacatcc ttgcataaaa atgataaaac
     9421 gaatacctgg cccatccaca gagccgaaac tttctgtaga atggatcatt cctgttactt
     9481 ttttatagtc tatttctgcc atttcatcac cttgtaaccg tttttatgac tttattatat
     9541 cattttcatg aaaaaaagac taagtaatct tagtcttttt caatattctg ttctatatca
     9601 gacaatagca ccttcaattt tgttatacga ccatctttaa ctttatcatt aatcaaagtg
     9661 atgtgtttat ctttactgtc tacttcgtaa gctacttttt cctcttggtt tggaatacta
     9721 cccacaccag ttaaataata acccgcaatt gtatcaacat catcactttc taactctgtc
     9781 tcaaaataat cattaaattc attaagcgtc atagttccta gaacaatata gatattctca
     9841 tcgatttccc tgacaaattg ttcagctgta tctgtttcat catcaatttc gccgacaatt
     9901 tcttccaata aatcttctag agttactaaa ccagcaacgc caccatattc atctagtaaa
     9961 atggccattt gattttgagt gttgcgcaat gcttttaaaa gatcatctac aaaaatcgtt
    10021 tctggaacaa aaagaggttc ctgaagtatt tttcgcaagt cgatagtatc aaaaccagtt
    10081 ttaaatcccg cttccaataa gcgcttggta tgtaaaacac ctacaactct atctttatcg
    10141 tcatcataaa cagggacacg agaaaagttt tgtgataaaa ttccttcaat atttgattgt
    10201 gcatcattgt tgatgtcaat catgaaagca tcagtacgcg gaaccataac ttcacgcgcc
    10261 atcatttcat ctagcgagaa aatcccttgc agcatctcaa tttcttcagc atccaaagtt
    10321 tcttcactat tcgtcaacat atattcaatt tcatcacgtg tcatcttctc gtcagcatca
    10381 tcaaaagtca tcggagtgat tctactcaat aagttagttg aagctgagag caaccataca
    10441 aaggggctta ctatttttcc tagaaagata ataatgggag ctgaaacaat tgctagacga
    10501 tctttcaaat tcatagcaat acgcttagga tataattctc ctagaacaat agagacatat
    10561 gttaaaaaga ttagcgcaat aatactccct gctgtacggg ctgttgctga atttcctagc
    10621 caccctgaaa tcacatgtcc tagtgaggca gataaactag ccccttgcaa aaggctaata
    10681 aaagtaatac caacttgaat tgttgataaa aaattattgg gattttcaag aacgtccaaa
    10741 agacgtcgat accttttgtc cccttcttca gctttctgtt ctactttaga gcggtttagc
    10801 gatactaaag ccatttcaga tgctgaaaag aaggcgttaa atagggttaa tattaataaa
    10861 ataacaaatt gtagtaacaa actctgactg ccagggtctt ccatggaaaa ttactcctaa
    10921 ataaaaatta tttgaattta gctgatagcg attaactcta ccaactgttc ttctattaga
    10981 taatgctatc attatatcat ataatagcac tgagcgcata gtcgataata ttgtttacgg
    11041 cattttatag caattatcat tttagctaat tgcctaaaga tagttacacc tcattttcat
    11101 atgattgtaa cttttctacc ataattaaaa aggggggcgt gttaacttga ttgagtggtt
    11161 gataaagcat tgtagcgaaa ttattctgtg gtaattgttc cacgaatgct atcaccgcat
    11221 ctttctcact ttttccacca tcatgaccat aataaaccat aagcgataga cgtccaccta
    11281 cttctaaaat atctaaaacg tttttgattg ccttgatagt tgtgtgaggt tttgtaataa
    11341 ctgttttatc cgctgagggt aagtatccca gattaaatat agctgctctt aaaggtgtat
    11401 gaacatactg ttccaaattt tcatgcccat ccaagataag ttcagcgtta gagatccctt
    11461 gctccgtgag ctttgctttc gtttttgcta ttgcttcttc ttgaatatca aatgcataaa
    11521 cttttttaga acttttcgct aaaaaaacag tatcattccc atttcccata gttgcatcaa
    11581 gcgttattgc ttctttatca ataacttctg ctaaaaaatc atgagaaaga tgaattggtc
    11641 ttttaatcat gttttacctc aactttacat ccttgatatg actgtctttt ctccatttct
    11701 ttgtcaatgg cattaagcac ttcccattta ttaaggctcc acatcggtcc aattaacata
    11761 tgacgtggcg catcaccagt aattcggtga ataacaatat gttttggaat aatctccagt
    11821 tggtcacaaa taatcgaaat atagtcttct tgacttaaca aacgtaaacg accttcatgg
    11881 taatctctct gcattcttgt attagtcata agatgcagaa ggtgtaattt tataccctga
    11941 atatcattat ccgtaacaca tcgacgaaca ttttccacca tcatatcatg tgtctcccct
    12001 gggagaccat ttattaggtg agaaacgatt tctactttag gagctaattc tcgtattctc
    12061 ttaacagttt ttttgtaaag gtcatatgaa tgtgctctat ttattaatgc agaagttact
    12121 tcataagttg tctgaagacc taattctaaa gtcacatgca ttctttcaga aagttcagcg
    12181 agatagtata tagtttcatc aggtaagcaa tccggccttg ttccgatgtt gatcccgata
    12241 actcctggct cattaatagc ctgttcgtaa cgctctttaa ttatctctaa cttagcatgg
    12301 gtattggtaa aattttgaaa atagactaag tatttattaa cctcaggcca ctttctatgc
    12361 atgaaatcaa tttctttata gaattgttca cgaataggag cttctggagc aactatagca
    12421 tcccctgaac cagaaactgt gcaaaaagtg caccctcctc tagcaactgt tccatctctg
    12481 ttaggacagt caaaaccagc atctataggt aatttaaata ttttttctcc aaagagttct
    12541 cgataataat cattaatcgc acgataacgt tttttcatag gataattgta tcacaatttt
    12601 aactaaaata acctcactac tacaataaaa ctaaaaaaga ttggaactaa ctgacgttcc
    12661 aatcttttat ttacttcact ttctttaacc aatccttggc taaaaagata tacgcagtta
    12721 gattcaaaat accataagca agtataaaac cagctaaaac atctgtcgga aaatgaaccc
    12781 ctaggtaaat acgagataac ccaattaaaa aaatgagcaa acccaaaata ccttggtaca
    12841 acagtttcca tatactctta ggcatatagt actgcaataa gataataata ctcccaaata
    12901 tcataaatgt tcccatcgag tgcccactgg gaaacgaata gccacctgca aatactaaat
    12961 gggttaaagt tggtcttact ctttgaaaaa taagttttaa agaaagtata catataccag
    13021 agataatagc atttactgcg ataaatctag cttgaggata ccacttctta aggtaacaga
    13081 aagtgacgct cataatcgca atagctatct ggcttacagt attaccaatc acagtgatta
    13141 acttgaaaaa tcttgtagaa agatttggca actgtcctct aacactttct tgaatagttt
    13201 ggtcaaatga aattacagtg tcgggccaat atttgatgac caatcctaaa ctgaaaaata
    13261 agataatagc aataaatgct tgaataagtt tactattttg acgagataac attagtcttt
    13321 ttatatcttt ctaatattgg caaacaagcc acgtaagtta gatagaaaac aatcgaaatt
    13381 aaaattccct caacgatatt aaatggaata accattgtta aaaggtaatt gcctacacca
    13441 ataaatgttc tgatatcaaa gttagcaaat atagcataca aaggaatcgc aaagacatag
    13501 ttgagagcta ccatagatac ggtcaagcta actgtaccaa atagactagc tttaataaaa
    13561 tcttttgcac tctctctatt tttccagaaa atagcgaaac ttgctaaaaa taaagctaga
    13621 gcaaccatat tcatcggtaa accaataaac gtttctggac cacgattagc aagtataact
    13681 tttaaaagtg atcttaataa gagtacacca taacttgatt tcaaatcaaa taaaataaaa
    13741 gcaactaaca tcggaaggat tgaaaaatca acctttaaaa attctgctcc tggtattaat
    13801 ggaaatgaaa ccatcatcaa tacaaaagat aaggcagaaa gaatggcgat tgtcaccatt
    13861 ttacgtgtat ttgtcataaa aaaattcctc caatttaaat aaattgaaag aagctccaaa
    13921 ggtaagcgta tgtacgcgaa aaaacctttg tcttctccca tccagacttt actgtcggtt
    13981 gtggaatctc accacatcag ctttcgctcg cggactgatg cttcacaact gacaaataag
    14041 ttggaagcga ttaccgccgg tcgggaatta caccctgccc tgaagacacc tatagcataa
    14101 caaaaaaaac ttgcaattgc aagtttttta attactaatt agtagtagtg attaaaaatc
    14161 atattaatac caaattacta tactgtatcg tttctttcag atttgctatt tttagttttt
    14221 cttaaaaaga taaacaaaat tcccaaaata atacaaccaa gaattgtcag tcctccacca
    14281 ataatcattc ctgttttagg aagaaatgat tgtggaaaaa gcggttgtga tggtttagga
    14341 tttgttggtg gaggagtttc tttttcgttt tctacctcta cttcctgtgt tttattagca
    14401 actacagcaa ctacagcatc cttcatagat atacggtaac cagttagtgc ttttgcttct
    14461 cgaaaaatat acttaccagg taataaacct tcaacctcaa tttctccctt atcatcagtt
    14521 actaatgaag taatcccatc ttgatcggtc gtaaatcgtc catttttaaa gcgaactggc
    14581 tgattctggt tatcgtataa tacaaatatt actccggata gccttttctt tatctttcct
    14641 tcttttgtat atttaataag ttttaatcgg cctgtttcaa cttttcgctt aggatttatc
    14701 tgtaattgat ttgataactt atcatctggt aattcaatat aaaaaggtac tattgttgaa
    14761 acgctttgat cagctttata agcacgacca aagtacgaac catttgggag tgctatcttt
    14821 gtctgaccat tagtatcagt aggagaagtc aagatactct tatacttctg gttcaattcg
    14881 ctatctgtca tttggctcaa taaatcaact tttaagttgt cagtcacagt ccataaacga
    14941 taagaaatcc cctcctctgt agtatttggc tgaagtccta tctgtgtgat tgttagttga
    15001 ttaggggtat cagcatttac actggctacc gaaaaaaacg ctaattgtac caatcctaaa
    15061 aagcaacata gtagaagtcc taaacttttt ctaatctttt tcatttttga tttccctttc
    15121 tctttctctc tttaaatttt cgttttaaat ataatagtaa agcgactaat ataagaataa
    15181 ctaggattga taagaggaaa taaagtttat agtgtgtttg caattctttc attaaatagt
    15241 tcttttcttt aacaggaggt acatacttga ttcgatgccc tctaactagt aaacgatgtg
    15301 aattaatcga ataaggtgta catgttagca aagtcgcata atccttacct ttaacaacca
    15361 ataatttaga aaaattatct ggctttacaa cacttatttg atcaacctta taggctaaaa
    15421 cttctttgat attatgaata taaaaaattt ttcctttttt aagtttatct aaatctgtaa
    15481 ataacttagc tttaggtaag ccgcgatgag ctgtgataac agtatgtgaa ctttttccac
    15541 caattggcaa ggaggttcct tcaaggtgtc ctgctccttt ttcaagaaca ctactggtag
    15601 tccccgcata gataggtaat ttttgcttga tagacggtat atcaatatat ccaatcattt
    15661 cagcaatctc aagcatgtgg gcgtattcag caataccttt tttttctttt tcagtatagg
    15721 gatctgatag gcggcttggg tccagtgttc tattataagc ttttgctaac tcaaatcgtc
    15781 tattaatctc tttagtattt aatttttggg tttgattatc aaagttagtt acttgattat
    15841 tagctttaat attatagtac caatttgaaa taaaaggata tgaggttatc aaaagaccaa
    15901 ctaagaacaa tagtatcagg cctacattca tccatcgatt taaaacgacc gatttcttaa
    15961 ggtttttctg aaattttcct cccattatga ttcaattcct tttctaacac ttgctaaacg
    16021 atttttttga cgttgacgtt ttattaacca aagtaaccaa gcaataataa ctaaagatat
    16081 atagaaaaga tatctataaa tcgtgtttaa atgaccttct ttcattaatt tttcatcaat
    16141 aagaccttta taaggaatac gatgtcccct tactaaaagt ctgtgtgtat tgaccataat
    16201 cggggtgcaa gttaataagg ttgcataatc atgtccagga acaaccaaca aatctgaaaa
    16261 gttatcgggt gtaacgactt ttatctgatc tacttgatat gctatcgttt cttttatgtt
    16321 ttgaatataa aacttatctc ctttttttaa ctttttaagg ttagaaaaga gttctttatc
    16381 tggaattcct gagtgcgctg ttataacggt atgtgtgcta tttcctccaa ttggaagaga
    16441 agtaccttct aaatgccctg ctcctttaga tagaactttt tgacttgaac ctgcaaatat
    16501 agggagtttt tgacctatct taggaactga aattgtgccg attttttcac ttacctctaa
    16561 catacgggcg tactctgcta cccccttttg aattcgtttc ttctcataag gatcttcaag
    16621 atggacatta tttaaagaat cattataagc ttgtgctaga gtcatacgac gattgatttc
    16681 tttctgacta agttttttag cagctctctc aaaatcctgt gtttgattat tagattctat
    16741 cgtatagtaa aaacgtgata ccactggata caataaaata gatagaccta ttagaaaaag
    16801 aatgataaaa ggaagatttg acttcttctt tttagttttt ttgttgatat ttttagtctt
    16861 cacgtcatct cctaaataat ggctcttgct tatgatctaa gagtacttct actgaaatac
    16921 ccttagatca taagcacagc tttaactgtg cttatacatc atcaaagact agccttaagc
    16981 ttcctctgat tgacgttttt tcatgataac tactgctcca agcataatgc ttaaaccaat
    17041 aattgtgaaa agaattgtac caataccacc tgtttgtggg atggttactt ttttgttttg
    17101 aacttgttgg gcatcttttt ttacagagcc tttatcatat gcgatgtcag ttgtagcccc
    17161 tttgctatat gatgtggcag ttacttcaaa gtttacatca cctgacaatg tcgcataacc
    17221 tgctggtgct tgagtttctt ccaagccata agtgccttta tccaaaccag taatttcaaa
    17281 ttgaccaccg gcgttagaga tcaatttaac aacattatca gcctttttat ctgcaaccca
    17341 ttcatatgag tagttagctt taacaaaagc gtcattgtaa gctttttgtt tttgatcaac
    17401 agtagccaat gctgttttac cttcttggcc ttcttgtttt tctttagtca agtcgttata
    17461 agctttaaca gcttcatcca atgctagttt agcagttttt acagctgcct ttgcttcagc
    17521 agttgctgca cctgctttac gtgccaagta tttgccttct ttcttaacga ggaaggtagc
    17581 tcctgccaag cgttcagtgt tagcttgatt tgttttcaca aatttacgtc cataagtcac
    17641 cacttttggt tctgatgggt tgattggagt tggatcattt gagtttttgt tgttcttgat
    17701 agtcacaaca ccatttttaa atgatacgta ttctggagtg tagccgctaa cacgttcgac
    17761 aacgcggtaa gttttagcat tatccaaacc tgtgaaagta tgttcaaagc gtgatggttt
    17821 tgttgcttcg tgtgaagcaa cgttcaccca tgtaccatcc gtttgttttt cttgcaaggt
    17881 aaagatagct ttaactgcaa cattagcatc agtaattgta ccatctactg cccagtcttt
    17941 aatgacttta atttcttggt tagctggagt accttcttgt ggttcacttt cttccgttgg
    18001 gttattacca tagtccaatt taacatcatt ggtttctgga atttcaacag tagtggagcc
    18061 gttcaccgta gctgagtaag tgatcttgat ttcaacatct ttgtcttttg cagctgctgc
    18121 tactgctgca agacctgttg cattcaaggc aagacggaaa ccttggtcat ctgttacgag
    18181 tttgtagttt aaaacaggaa aatcttcacc atccaatgtt actttaacgt tgttgttgaa
    18241 cgtcaaacct ttagtcatgc tatcagtcca aaccagtttc ttatagtctg agcctttaag
    18301 aatttttgtt ccaactatgt attctttttt gtcaccaact gtcgctgaga caacaccttt
    18361 gtcttttcgg ttgtcagtat aatcaagatc tttatctgca aagttcttat ctacttgtgg
    18421 ttttgtttca gtattctttg gataaatgtg agcatcttta acaacacctt ggttgtttac
    18481 caatggcaga gtgattttaa ctggaactgc ttttgaatca gccaagatag aaccgttgtt
    18541 atcgtagttt gatttttctt tcaattcaac gatttggtaa attcctttta acttagcagt
    18601 gttaaaaaca aaaccattgt cttttgttaa ccctgaaaga acagcaccac cttcagcatc
    18661 tttagcttcc aaagtatcga cttccttacc attttcagta atgaattttg taccagtttc
    18721 atttttgaaa acaaagaaag cacccttgat ttctttagca tcggttgagc caaaataaga
    18781 tttaaggtca ttaatttgtt taccaacata atcgctatca ttcttacctt ttgtaccttc
    18841 agtaaagtta tcaaatgcag cttgtggcat gacaatcttg tgcaaggtca cagtatcagt
    18901 tgttgcgtcg tccgcaaacg ctggtgcaac tgagaatagt gacgttaaga tcaatagcag
    18961 tgtcgagaac attgtaagac atttgttgat ttttttcatt tctatctcct tcttattcta
    19021 gttaatcaac atagataata atatacggat tataatatta ccgcagcacc actcctttcg
    19081 aattatattt aattaattaa gaatactaaa acacatgatt cttaatcttt ttctctagat
    19141 atatcactag atttcttata tcttttccaa atataaattc cacctgcaat agacatcata
    19201 gatccaccta ttaaaatgaa agatagaatt cctttcccac ctgtcatcgg aataattcct
    19261 tttggtggaa tatgcgtgtt ggtaattaaa
//
LOCUS       DATRNK010000040        19187 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.040, whole genome shotgun sequence.
ACCESSION   DATRNK010000040 DATRNK010000000
VERSION     DATRNK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19187
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.040"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            61..975
                     /locus_tag="VBC52_001346"
     CDS             61..975
                     /locus_tag="VBC52_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO6882931.1"
                     /translation="MKSAYIFFNPKSGKDEQALAQEVKSYLIEHDFQDDYVRIITPSS
                     VEEAVALAKKASEDHIDLVIPLGGDGTINKICGGVYAGGAYPTIGLVPAGTVNNFSKA
                     LNIPQERNLALENLLNGHVKSVDICKVNDDYMISSLTLGLLADIAANVTSEMKRKLGP
                     FAFVGDAYRILKRNRSYSITLAYDNNVRSLRTRLLLITMTNSIAGMPAFSPEATIDDG
                     LFRVYTMEHIHFFKLLLHLRQFRKGDFSQAKEIKHFHTNNLTISTFKRKKSAIPKVRI
                     DGDPGDQLPVKVEVIPKALKFIIPNSLP"
     gene            1076..1930
                     /locus_tag="VBC52_001347"
     CDS             1076..1930
                     /locus_tag="VBC52_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO6882932.1"
                     /translation="MTTFTAKFIDQEWEVPVESGRYHMIVGEFCPYAQRPQIARQLLG
                     LDKHISISFVDDVPSDIGLIFSQPEQVTGAKSLRDIYHLTDPTYKGPYTIPILIDKTD
                     NRIVCKESADMLRLFTTDFSDLHQEDAPVLFSQETASLIDNDIKDINNNFQSLMYKLA
                     FLDKQADYDTYSKEFFTFLDQKEHLLGQRPFLLGDNLSEVDIHFFTPLVRWDIAGRDL
                     LLLNQKALEDYPNIFSWAKTLYNDFNLKTLTNPQSIKNNYYLGKFGRAVRHHTIVPTG
                     PNMVKWEK"
     gene            complement(1976..2194)
                     /locus_tag="VBC52_001348"
     CDS             complement(1976..2194)
                     /locus_tag="VBC52_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEO6882933.1"
                     /translation="MIKLYYEDAYDTERCQVFENPNDFIRVLMGCVTVPDNYPITKMT
                     YKDKNIAYSGTYGDLFKTFIEFDWSTLD"
     gene            complement(2262..3257)
                     /locus_tag="VBC52_001349"
     CDS             complement(2262..3257)
                     /locus_tag="VBC52_001349"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEO6882934.1"
                     /translation="MTNRKDDHIKYALKYQSPYNSFDDIELIHSSLPKYNVNDIDLST
                     HFAGQSFEFPFYINAMTGGSEKGKAVNHKLAQVAQATGIVMATGSYSAALKNDEDDSY
                     PTTDLYPDLKLATNIGLDKPLPAAESTVKAMNPIFLQVHVNVMQELLMPEGEREFHMW
                     RSHLKEYVDNIQCPLILKEVGFGMDLQSIKDAYDIGITTVDISGRGGTSFAYIENQRG
                     RDRSYLNTWGQTTAQSLINAQSMMDKMDILASGGIRHPLDMVKCLVLGAKAVGLSRTV
                     LELVERYPVDDVIAILNSWKEDLRMIMCALNCKKITDLRQVNYILYGQLKEANAK"
     gene            complement(3254..4246)
                     /locus_tag="VBC52_001350"
     CDS             complement(3254..4246)
                     /locus_tag="VBC52_001350"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEO6882935.1"
                     /translation="MVKVQTGGKLYIAGEYAILYPGQVAILKNIPIYMTALATFADNY
                     SLYSDMFNYTASLQPDKQYSLIQETILLMEEWLINFGKNIKPIHLEITGKLERYGLKF
                     GIGSSGSVVVLTIKAMAALYEIEMPSDLLFKLSAYVLLKRGDNGSMGDIACIAYEHLI
                     SYSAFDRRAVSKMIETKPLEQVLEAEWGYRITKIQASLEMDFLVGWTMQPSISKEMIN
                     IVKSTITQRFLDDTNYQVVQLLSAFKEGDKEAIKRCLEEISLLLFNLHPSIYTDKLQK
                     LKEASKGLDIVTKSSGSGGGDCGIAISFNKNDNQTLIKRWESAGIELLSKETLS"
     gene            complement(4239..5183)
                     /gene="mvaD"
                     /locus_tag="VBC52_001351"
     CDS             complement(4239..5183)
                     /gene="mvaD"
                     /locus_tag="VBC52_001351"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEO6882936.1"
                     /translation="MDGKSISVKSYANIAIIKYWGKADAEKMIPATSSISLTLENMYT
                     ETRLTTLGKDAKKDEFYISGVLQNDHEHDKMSAILDRFRQNKSGFVKIETTNNMPTAA
                     GLSSSSSGLSALVKACNDFFGTNLSQSQLAQEAKFASGSSSRSFFGPVAAWDKDSGDI
                     YKVHTDLDLAMIMLVLNDKRKPISSREGMKICTETSTTFNEWVRQSEQDYQDMLVYLK
                     NNDFQKVGQLTERNALAMHSTTKTATPAFSYLTEETYKAMDVVKKLREKGHECYYTMD
                     AGPNVKVLCLRQDLEALAAILEKDYRIIVSTTKELADG"
     gene            complement(5165..6043)
                     /gene="mvk"
                     /locus_tag="VBC52_001352"
     CDS             complement(5165..6043)
                     /gene="mvk"
                     /locus_tag="VBC52_001352"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEO6882937.1"
                     /translation="MKEKFGIGKAHSKIILMGEHSVVYGYPAIAIPLKNIEVTCLIEE
                     APQLIALDMTDPLSTAIFAALDYLGKTSSKIAYHIESQVPERRGMGSSAAVAIAAIRA
                     VFDYFDEDLEADLLECLVNRAEMIAHSNPSGLDAKTCLSENTIKFIRNIGFSTVPMHL
                     NAYLVIADTGIHGHTKEAVDKVKSSGEAVLPFLKELGYLAEASEDAIHKSDSKQLGSL
                     MTKAHQSLKQLGVSSLEADHLVEVAISCGALGAKMSGGGLGGCIIALVKEKREAERLS
                     QQLEREGAVNTWTEKV"
     gene            complement(6159..7388)
                     /locus_tag="VBC52_001353"
     CDS             complement(6159..7388)
                     /locus_tag="VBC52_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6882938.1"
                     /translation="MFRNLRLRFIGIAALAILVVLFSVVGVLNSANHYQTKNEIYRVL
                     TILADNNGRIPNKLEFSKELGDDLSTDAIFQFRYFSARTDAKGNVTSFDSRNIFEVSD
                     RQIKNYAKRIVSQNSDSGHITYNFSTYSYLLKKVGKNDYLVVFLDTTNQYLDNQRLLQ
                     LSIWMSLASFIVFMVIVSVLSGRVILPFVANYEKQRRFITNAGHELKTPLAIISANNE
                     LVEMMSGESEWTKSTNDQIQRLTGLINGMVSLARFEEQPDISMVDLDFSHITKDAAED
                     FKGPIIKDGKDFIMSIQPGIHVKAEEKSLFELVTLLVDNANKYCDPMGTVTVKLSRSS
                     RLRRAKLEVSNTYKNGKDIDYSKFFERFYREDESHNNKKSGYGIGLSIVTSLVYLFKG
                     SIDVNYKHDTITFVIYI"
     gene            complement(7388..8074)
                     /locus_tag="VBC52_001354"
     CDS             complement(7388..8074)
                     /locus_tag="VBC52_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6882939.1"
                     /translation="MRVLIAEDEEQMSRVLSTAISHQGYVVDVAYDGQTAIDLANQNA
                     YDVMVMDVMMPVKTGVEAVKEIRQSGNKSHIIMLTAMAEIDDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMSRRLEDFTPNVLSLGRVTLSVGEQELQCENTIRLAGKEAKMLAFF
                     MLNHDKELSTQQLFEHVWGADKDQEDVDEGYVFIYVSYLRQKLQAISANLEIIGQEGS
                     SYKLSEINGG"
     gene            complement(8076..8702)
                     /locus_tag="VBC52_001355"
     CDS             complement(8076..8702)
                     /locus_tag="VBC52_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO6882940.1"
                     /translation="METNIYGDYGRYLPLILEDFSQRIQLENDKAKVETGYKLYEHII
                     GRIKTSDSMIEKCRRKQLPVTVDSALKAIRDSIGVRIICGFVNDIYQIVERIKSFDDC
                     RIVVEKDYIQHVKPNGYRSYHVILEIDTPYPDCLGNSEGKYYIEIQLRTIAQDSWASL
                     EHQMKYKHDIENPERIVRELKRCADEMASVDLTMQTIRQLIESGTKKE"
     gene            complement(8810..10564)
                     /locus_tag="VBC52_001356"
     CDS             complement(8810..10564)
                     /locus_tag="VBC52_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6882941.1"
                     /translation="MKTKSQKTSFFSNLSPYLKGYKLLFALAIVLATLSSLTTVIGPD
                     RLKEMTDLMVKGLAGNIDLAKIGHIAGQLAVLYVLGALVSYLASFIVTTIIQKFAQGM
                     RNAIADKINKIPLSYFDSHTQGDTLSRVTNDVDLMTQSFNQSLVTMVASTVLLIGSLI
                     MMFKTDWHLATTAIISVFSGFALSSIIMVKSQPLFKKQQMNLANISGYVEEIYSGHNI
                     VTSHNARQQAKSDFQLLNDKIHSSMWKSQFFSGIMMPLMQFIGNFGYVMVCVVGAMLA
                     IKGTITMGTIVAFMTYVRIFTQPIGQIAQGITQLQSASAAMGRVFEFLNETNMQEDKH
                     KVLNLPKFKGYVSFEHVKFGYSDNKTIIHDFTSEAQPGQKVAIVGPTGAGKTTIVNLL
                     MRFYDVNSGSISIDDINIQDMKRSQVHDAFSMVLQDIWLFEGTIKENLIFNQTTISDD
                     EVQEAAKAVGVHHFIMTLPKGYDTVLDDAVTLSVGQKQLLTIARALLKNSPLLILDEA
                     TSSVDTRTEELIQKAMDKLMEGRTSFVIAHRLSTIKNADLILVMKDGNIIEQGSHDVL
                     MSQSGFYADLYNSQFESA"
     gene            complement(10554..12371)
                     /locus_tag="VBC52_001357"
     CDS             complement(10554..12371)
                     /locus_tag="VBC52_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6882942.1"
                     /translation="MFNIFKRLNGKEWLMIVASTILVCLAVWMDLKTPEYMSDITRML
                     QTKGTKLEALWDPGLKMLAFSFGSFLVAVMVGFLGARTAASFTSRLRSDIFNHVMDYS
                     DAEIKRFSIPSLLTRTTNDLTQLQILIVMGMQVVTRGPIMAIWALTKIWGKSDQWTGA
                     VGVAVIIVMILLSVLLLLAFPRQRQIQGFTDALNATTREALSGVRVIRAYNAEKYQTD
                     KFKRENDDLTKLNLFVYRLMSLMNPVMTVVSSGLTLAIYWIGSYLINDISVPKVTGTS
                     VSVLAAKSAITDRVDLFSNMVVFSSYAMQVVMGFMMMVAIFIIMPRALVSVRRINEVL
                     TLAPTVTFKENSHIQSAQKGQIEFQDVSFHYSKDSEAVIEHVSFKADTGQTVAFIGST
                     GSGKSTLVNLVPRFYDATEGAIKLDGIDIKEYSHNDLNNKIGYIPQKAVLFSGTVRSN
                     MILGESGREELSDEEIWQALELAQAKEFIEAKEKGLDTEVAQGGSNFSGGQKQRLAIA
                     RALARHPEILIFDDSFSALDYKTDHILRHELKEKTAAMTKLIVAQRISTIMDADLILV
                     LDQGKVVGQGTHKELLSNNEVYQEIAYSQLSKEELENEN"
     gene            complement(12418..12858)
                     /locus_tag="VBC52_001358"
     CDS             complement(12418..12858)
                     /locus_tag="VBC52_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6882943.1"
                     /translation="MENPLQKARILVNQLEKYLDRYAKEYDVEHLAGPQGHLVMHLYK
                     HPDKDMSIKDAEEILHISKSVASNLVKRMEKNGFIAIVPSKTDKRVKYLYLTHLGKQK
                     ATQFEIFLEKLHSTMLAGITKEEIRTTKKVIRTLAKNMAMEDFD"
     gene            13127..15199
                     /locus_tag="VBC52_001359"
     CDS             13127..15199
                     /locus_tag="VBC52_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEO6882944.1"
                     /translation="MKKKIILKSSVLGLVAGTSIMFSSAFADQVGVQVIGVNDFHGAL
                     DNTGTANMPDGKVTNAGTAAQLDAYMDDAQKDFKQTNPNGESIRVQAGDMVGASPANS
                     GLLQDEPTVKTFNAMNVEYGTLGNHEFDEGLAEYNRIVTGKAPAPDSNINNITKSYPH
                     EAAKQEIVVANVIDKVNKQIPYNWKPYAIKNIPVNNKSVNVGFIGIVTKDIPNLVLRK
                     NYEQYEFLDEAETIVKYAKELQAKNVKAIVVLAHVPATSKDDIAEGEAAEMMKKVNQL
                     FPENSVDIVFAGHNHQYTNGLVGKTRIVQALSQGKAYADVRGVLDTDTQDFIETPSAK
                     VVAVAPGKKTGSADIQAIVDQANTIVKQVTEAKIGTAEVSGMITRSVDQDNVSPVGSL
                     ITEAQLAIARKSWPDIDFAMTNNGGIRADLLIKPDGTITWGAAQAVQPFGNILQVVEI
                     TGRDLYKALNEQYDQKQNFFLQIAGLRYTYTDNKEGGEETPFKVVKAYKSNGEEINPD
                     AKYKLVINDFLFGGGDGFASFRNAKLLGAINPDTEVFMAYITDLEKAGKKVSVPNNKP
                     KIYVTMKMVNETITQNDGTHSIIKKLYLDRQGNIVAQEIVSDTLNQTKSKSTKINPVT
                     TIHKKQLHQFTAINPMRNYGKPSNSTTVKSKQLPKTNSEYGQSFLMSVFGVGLIGIAL
                     NTKKKHMK"
     gene            complement(15236..15646)
                     /locus_tag="VBC52_001360"
     CDS             complement(15236..15646)
                     /locus_tag="VBC52_001360"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO6882945.1"
                     /translation="MIKPIVRDTFFLQQKSQMASRADVSLAKDLQDTLHANQNYCVGM
                     AANMIGSLKRVIIINVGITNLVMFNPVLVAKSDPYETEESCLSLVGCRSTQRYRHITI
                     SYRDINWKEQQIKLTDFPAQICQHELDHLEGILI"
     gene            complement(15716..17065)
                     /gene="gdhA"
                     /locus_tag="VBC52_001361"
     CDS             complement(15716..17065)
                     /gene="gdhA"
                     /locus_tag="VBC52_001361"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEO6882946.1"
                     /translation="MTSGKTYVASVLEKVKKQNEHEEEFLQAVEEVFESLVPVFDRYP
                     QYIEENLLERLVEPERVISFRVPWVDDKGQVQVNRGYRVQFSSAIGPYKGGLRFHPTV
                     TQSIVKFLGFEQIFKNSLTGLPIGGGKGGSNFDPKGKSDNEVMRFTQSFMTELQKYIG
                     PDLDVPAGDIGVGGREIGYLYGQYKRLNGYQNGVLTGKGLTYGGSLVRTEATGYGAVY
                     FAKEMLAARGQDLTGKVALVSGSGNVAIYATEKLQELGATVVAVSDSSGYVYDPDGID
                     LETLKQIKEVERARIVKYTEKHPKANFTPADQGSIWSIKADLAFPCATQNELDEEDAK
                     LLVENGVLAVTEGANMPSTLGAIKVFQKAGVAFGPAKAANAGGVAVSALEMAQNSSRR
                     AWTFEEVDQELQRIMKTIFVNASEATDEFGDSGNLVLGANIAGFLKVAQAMSAQGIV"
     gene            17233..17742
                     /locus_tag="VBC52_001362"
     CDS             17233..17742
                     /locus_tag="VBC52_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEO6882947.1"
                     /translation="MKWFPLVGGIIALLAGFYLFINPVTVVATLGWFIALFIFIYGIS
                     NLVRYLQADSYHRSPWYLVQSILAIIFGIILLSSSAMSLSNTVMTILAYWLLVSGILR
                     LLGGFQMQKINFPGAKSYKRSAYIALILGIILLIQPTLSAAIIGRFVAFILIAIGASA
                     IYTFTRINY"
     gene            complement(17783..>19187)
                     /locus_tag="VBC52_001363"
     CDS             complement(17783..>19187)
                     /locus_tag="VBC52_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6882948.1"
                     /translation="DKHQFGDMLGRFTSDVETVSNALQQSFLQIINAFLSIILVVVMV
                     LYLNVPLAMIIIACIPVTYFSAQAILKRSQPYFKEQAKILGELNGFVQEKLTGFNIIK
                     LYGREEASSQEFRDITDNLRHVGFKASFISGIMMPVLNGISDFIYLIIAFVGGLQVIA
                     GTLTIGNMQAFVQYVWQISQPVQTITQLAGVLQSAKSSLERIFEVLDEEEEANQVTEK
                     LSHDLTGQVSFHGVDFHYSPDKPLIRDFNLNVEPGQMIAIVGPTGAGKTTLINLLMRF
                     YDVSEGAITVDGHDIRHLSRQDFRQQFGMVLQDAWLYEGTIKENLRFGNLEASDEDIV
                     AAAKAANVDHFIRTLPGGYNMVMNQESSNISLGQKQLLTIARALLADPKILILDEATS
                     SVDTRLELLIQKAMKKLMEGRTSFVIAHRLSTIQEADNILVLKDGQIIEQGNHQHLLA
                     DKGFYYELYNSQFSNSK"
BASE COUNT         6156 a         3808 c         3019 g         6204 t
ORIGIN      
        1 tttttgctaa aacatgatat aataataatc atctaaatct tagagagaag gcgtggacat
       61 atgaaaagtg cttatatctt ttttaatcca aaatctggta aagatgaaca agcattggca
      121 caagaagtaa aatcttattt aatagaacat gattttcaag acgattatgt tcgtatcatt
      181 acaccttcat ctgttgagga agctgtcgcc ttagcaaaaa aagcatcaga agaccatatc
      241 gacttggtta ttcctttagg gggagatggc actattaata aaatctgtgg tggtgtctac
      301 gctggtggtg cttatcctac tatcggacta gtacctgccg ggaccgtcaa taacttttca
      361 aaagctttga acatcccaca agagaggaat ctcgctcttg aaaaccttct aaacggtcat
      421 gtaaagtccg tagatatctg taaagttaac gacgattaca tgattagcag tttaacactt
      481 gggctccttg ctgatattgc tgctaatgtc acttctgaaa tgaagcgtaa attaggacct
      541 tttgctttcg taggtgatgc ctaccgtata ttaaagcgta atcgatcata ttctattact
      601 cttgcttacg ataataatgt acggtcttta cgcacgcggt tactacttat taccatgacc
      661 aattcgattg ccggtatgcc agccttctct ccagaagcaa caattgatga tggacttttt
      721 agagtctaca ctatggaaca tattcacttc ttcaaattat tgttgcatct aagacaattt
      781 cgtaagggag attttagtca agcaaaggag ataaaacatt ttcataccaa taatctgact
      841 atttcaactt ttaaacgtaa aaaatcagct atccctaaag ttcgtattga tggcgatcca
      901 ggtgaccaat tgcctgtaaa agttgaagtc atccctaaag ccttaaagtt tatcattcct
      961 aactcattac cataatataa aaggacctag acataagtat tagagtaaat actatctgtc
     1021 taggttcttt tttgttatac ttaaagcaat aaatatcatt gaaaggaagt atacgatgac
     1081 aacatttact gctaaattta ttgaccaaga gtgggaagtc ccagtagaat caggtcgcta
     1141 ccatatgatt gttggggaat tttgtcctta tgcacaacgt ccgcagattg cacgccaact
     1201 gcttggtttg gataaacata tttctatcag ttttgtggat gatgttccaa gtgatatagg
     1261 acttattttt agccaaccag aacaggttac tggtgctaag tcgcttcgtg atatttatca
     1321 tttgactgac ccgacctaca aaggtcctta tactatccca attttaattg acaagactga
     1381 taatcgtatt gtctgtaagg aatctgcaga tatgcttcga ctttttacta cagatttctc
     1441 tgatttgcat caagaggatg ctcctgtcct tttcagccaa gaaactgctt cacttattga
     1501 taatgatatt aaagacatta ataataactt ccaaagctta atgtataagt tagcttttct
     1561 tgataaacaa gcagattatg atacttatag taaggaattc ttcacttttc tagatcaaaa
     1621 agaacactta ctaggacaac gtcctttctt gctcggagat aatctaagtg aggttgatat
     1681 tcatttcttt acaccattag tccgctggga tattgctggg cgtgatcttc tactacttaa
     1741 tcagaaagcg ctagaagact acccaaatat tttttcttgg gctaaaactt tatacaatga
     1801 ctttaattta aaaacattaa ccaatccaca atccataaaa aataactatt acttaggcaa
     1861 atttggaaga gctgttcgtc atcataccat cgtaccaaca ggtccaaata tggtaaaatg
     1921 ggaaaaataa agctaaaaag ctaaggcaat tcacagcctt agctttttag ctatactagt
     1981 ccaaagtgga ccaatcaaat tcaataaaag tcttgaagag atctccataa gtaccactat
     2041 aagcaatatt tttatcttta taagtcattt tcgtaatagg ataattatca ggaacagtaa
     2101 cacatcccat taaaactcga ataaaatcat ttggattttc aaaaacttgg catcgctcag
     2161 tgtcgtaagc atcttcataa tacagtttaa tcatcttatt acctctgacg ttttacaagg
     2221 aaactttgac aggaagctat atctacttca caaaaataat atcatttagc attggcttcc
     2281 ttcaattgac catacaatat ataattgact tgtcgtaaat cagttatttt tttacaattc
     2341 agtgcacaca ttatcattct taaatcttct ttccaagaat tgagaattgc aataacatca
     2401 tctacaggat aacgctcaac taattctaaa actgttctag aaagtccaac agctttagca
     2461 cccaacacta aacattttac catatctaaa ggatgacgaa tgccaccaga tgctaggata
     2521 tccattttgt ccatcataga ttgagcgttt attaaagatt gagctgttgt ttgcccccag
     2581 gtattgagat acgaacggtc gcgtccacgt tgattttcga tgtaagcaaa actagtcccc
     2641 ccacgccccg aaatatctac agttgtgata cctatatcgt aagcatcctt tattgattgt
     2701 aaatccatcc caaaaccaac ctcttttaag ataagtgggc attggatatt gtcgacgtat
     2761 tcttttaagt gagaacgcca catgtgaaat tcacgctctc cttcaggcat caaaagttct
     2821 tgcatcacat taacatgaac ttgtaaaaaa atggggttca tagccttaac ggttgactct
     2881 gctgctggta aaggcttgtc taaccctata tttgtcgcca gttttaaatc aggatacaag
     2941 tctgttgttg ggtaggaatc atcttcatcg tttttgagag ctgccgaata tgaacctgtc
     3001 gccataacaa tccctgtagc ttgagcaacc tgtgccaatt tatgattgac tgctttccct
     3061 ttttcactac ctcctgtcat agcattgata taaaaaggaa attcaaagct ttgaccagca
     3121 aaatgtgttg ataaatcgat atcgttgaca ttatattttg gtaaggatga atgtattaac
     3181 tcaatatcgt caaaactatt ataaggcgac tgatatttaa gagcatattt tatgtgatca
     3241 tcttttcgat ttgtcatgac aatgtctcct tactaagtag ttcaatacca gcactctccc
     3301 aacgcttgat tagagtttga ttatcatttt tattaaagga tatggctata ccacaatcgc
     3361 ctcctccaga tcctgaagat tttgtgacta tatctaggcc tttgcttgcc tctttaagct
     3421 tttgaagttt atcggtgtaa atagatggat gaagattaaa taataacaag ctaatttctt
     3481 ctaaacatct cttaatcgct tctttatctc cttctttaaa ggctgaaagt aactggacaa
     3541 cttgataatt tgtatcatct aaaaaacgct gcgtaatagt tgatttaact atatttatca
     3601 tttcttttga aatggacggt tgcattgtcc atcccactaa aaaatccatc tccaatgaag
     3661 cttgtatttt ggtaatacga taaccccatt cagcttccaa tacttgttcc aagggttttg
     3721 tttcaatcat ttttgaaaca gccctacgat caaaagctga ataagaaatt aaatgctcat
     3781 aagcaataca agcaatatct cccattgagc cattatcacc tctttttaag agaacgtagg
     3841 cagataattt aaaaagcaag tcgcttggca tctctatctc ataaagcgca gccatagctt
     3901 taatagtcaa aacgacaaca cttccgctag agcctatacc aaatttaagt ccatagcgct
     3961 ctaattttcc cgtaatttcg aggtgaatcg gttttatgtt tttgccaaaa ttaatcaacc
     4021 attcttccat aagcaatatc gtttcctgaa tgagactata ttgcttatca ggctgcaagc
     4081 ttgccgtata gttaaacata tcagaataaa ggctgtagtt atctgcaaaa gtagctaatg
     4141 cagtcatata aataggaatg ttctttaaaa tagctacttg tccaggatac agtatcgcgt
     4201 attcacctgc aatatataat ttgccacctg tttgcacctt aaccatctgc taattccttt
     4261 gtcgttgaaa caataatacg ataatctttc tccaagatag cagctaatgc ctcaaggtct
     4321 tgacgtaagc aaagtacttt aacatttgga cccgcatcca tcgtataata acattcatga
     4381 cctttttcgc gaagtttctt cacaacatcc atagctttat atgtctcttc tgtcaaatat
     4441 gaaaaggctg gagttgctgt tttagttgtt gagtgcattg caagtgcatt acgctctgtc
     4501 agttgaccta ccttttgaaa atcattattt tttaggtata ctaacatatc ttgatagtct
     4561 tgttcagatt gccttaccca ttcattaaaa gtcgttgagg tttcggtgca gattttcata
     4621 ccttcacggc ttgaaatcgg ctttcttttg tcatttaaga ccagcataat catagctaaa
     4681 tctaaatcag tgtgaacttt atagatatca ccactatctt tatcccaagc agcaacaggt
     4741 ccaaaaaaag agcgtgaaga agagcctgaa gcaaacttgg cttcttgagc taactgtgat
     4801 tggctaaggt tagtaccaaa aaaatcattg caagccttta ctaaggctga taaaccactt
     4861 gatgacgatg ataagcctgc agctgttggc atattattag ttgtttcaat ctttacaaag
     4921 ccggacttat tttgtcgaaa gcgatccaag atagctgaca ttttgtcatg ttcatgatca
     4981 ttttgaagaa caccagagat gtaaaattca tctttcttag catccttccc aagggtagtc
     5041 aaacgtgttt cagtgtacat gttttcaagt gttagagaaa tactactggt agctggaatc
     5101 attttttcag catctgcttt tccccaatat ttgataatag caatgttagc ataagactta
     5161 acacttatac tttttccgtc catgtgttga cagctccttc tctttctaat tgctgactta
     5221 agcgctcagc ttctcttttt tcttttacaa gtgcgataat acaaccacct aatccgccgc
     5281 cactcatttt agcgcctaga gcaccacaac taatagctac ctctaccaga tgatcagctt
     5341 ctaaacttga gacacccaat tgttttaaac tttgatgcgc tttagtcatc aagctaccaa
     5401 gttgtttgga atcactttta tgaatagcat cctctgatgc ctctgctaaa taacctaatt
     5461 cttttaaaaa gggtagtact gcctcgccag atgatttcac cttatccact gcttctttag
     5521 tatgcccatg aattcctgta tcagcaataa ctaaataagc attcaaatgc atgggtactg
     5581 ttgaaaaacc aatattgcgg ataaatttaa tagtattttc actaagacaa gtttttgcat
     5641 ctaatccact cggatttgaa tgtgctatca tttctgctcg attaacaagg cattctaaca
     5701 aatctgcctc caaatcttca tcaaaataat caaaaactgc acgaatagct gcaatagcaa
     5761 ctgcagctga tgatcccata ccacgtcttt ctgggacttg tgactcaata tgatatgcaa
     5821 ttttagaaga agttttacct aaatagtcca aggcagcaaa aatggctgtc gacaaaggat
     5881 cagtcatatc taatgctatt aattgaggag cctcttcaat aaggcaagtt acttcaatat
     5941 tttttaaagg aatagcaatg gctggataac catatactac tgagtgctca cccatcaaga
     6001 taatcttgct atgagccttt cctataccaa acttttcttt catctttttc tctttacaat
     6061 tctattacct tactatttta atataaatcc ttaaaaaaag cgactttaac gtgataataa
     6121 cataaattaa aggaactaca attgcagtcc ctttaatctt aaatgtaaat gacaaaggta
     6181 atggtatcat gtttataatt cacatcgata cttcctttaa atagatacac caaactagtt
     6241 acgatagaaa gaccaatacc atatcccgat tttttgttat tatgtgactc atcctctcga
     6301 taaaatcgtt caaaaaactt gctatagtct atgtctttac cattcttata agtatttgaa
     6361 acttcaagct tagctcgtct caaacgactt gaacgtgata acttaactgt cacagtcccc
     6421 atcggatcac aatacttatt agcattatca accaataatg taaccaattc aaataatgat
     6481 ttttcttcag ctttgacatg aatacctggt tggatactca ttataaaatc tttaccatct
     6541 ttaatgattg gacctttaaa atcttctgca gcatcttttg taatatgtga aaagtctaag
     6601 tcaaccatac ttatatcagg ctgttcttca aagcgggcaa gactgaccat gccattaatt
     6661 agacccgtta aacgttggat ttgatcattt gtagatttag tccactctga ttcaccactc
     6721 atcatttcca ccaattcatt attagcggag atgatagcaa gtggagtttt tagttcatgt
     6781 cctgcattgg taataaaacg cctttgcttt tcatagttag caacaaatgg taagataact
     6841 ctccctgata aaactgaaac aattaccatg aagacgataa aacttgccaa ggacatccat
     6901 attgaaagtt gcaataatct ttgattatca agatattggt tggttgtatc taaaaatacc
     6961 actaaataat catttttacc aacctttttt agtagataag aataagtact aaaattatag
     7021 gtaatatgac ctgaatcact attttgtgat acgatacgct tggcataatt ttttatttgc
     7081 ctatcgctta cttcaaagat atttctagaa tcaaaactag tcacgttacc ttttgcatct
     7141 gttcgcgcac tgaaataacg gaattggaaa atagcgtccg ttgataggtc atcacctaat
     7201 tctttactaa attcaagttt attcggtata cgtccattgt tgtctgcaag gatagttaac
     7261 acacgataaa tttcattctt cgtttgatag tgattagcag agttgaggac accgactact
     7321 gaaaataaaa caacaagaat agctagcgca gctattccga taaaacgaag tcgtaaattt
     7381 ctaaacatta gccaccattt atttcactta atttatagct actaccttct tgaccaataa
     7441 tctccaagtt agcactaatt gcctgcaatt tttgtctcaa ataagaaaca tagataaaga
     7501 cgtatccctc atctacatct tcttggtcct tatctgctcc ccagacatgt tcgaaaagtt
     7561 gttgtgtaga taattcttta tcatggttca acatgaaaaa tgctaacatt ttagcttctt
     7621 tacccgctag acgaatagtg ttttcacatt gcaactcttg ttcaccgact gataaggtta
     7681 ctcttcccaa tgacaataca ttaggtgtga aatcttccaa tcttcgggac atagagcgaa
     7741 gccttgctaa taattctttt agagagaagg gttttgttaa ataatcatct gcaccagcat
     7801 ctagacctgt tactctatca tcaatttctg ccatggctgt tagcataata atgtgagatt
     7861 tatttcctga ttgtctaatt tctttgacag cctcaacacc tgttttaaca ggcatcatga
     7921 catccataac catgacatca tatgcatttt gattagctaa atcaatagca gtctgaccat
     7981 cgtaggcgac atcaacaaca taaccttggt gactaattgc cgtactaagg acacgtgaca
     8041 tctgttcttc atcttctgca attaatactc tcattttatt ccttctttgt tccactctct
     8101 atcaattgac gaatcgtttg catcgttaaa tcaacagatg ccatttcatc agcacaacgt
     8161 tttaattctc ttacaattcg ttcgggattt tcaatatcat gcttgtattt catctgatgt
     8221 tctaaactag cccaagaatc ttgcgcaatt gtacgcaact gaatctcaat gtaatattta
     8281 ccttctgaat tacccaaaca gtctggatag ggagtatcaa tttctaaaat cacatggtaa
     8341 gagcgatacc catttggctt aacatgctgg atatagtctt tttcaactac aatacgacaa
     8401 tcatcaaatg acttaatacg ttctacaatt tgataaatat cgttaacaaa accacaaata
     8461 atacggactc caatactatc tctaatagct tttagtgcag aatctactgt tacaggtaac
     8521 tgtttacgac gacatttctc tatcatacta tctgatgtct taatacgacc aatgatatgt
     8581 tcgtacaatt tatagcctgt ttcaacctta gctttatcat tttctaattg tatacgctgg
     8641 ctgaaatctt ctaaaattaa tggtagatat cgaccataat ctccataaat atttgtttcc
     8701 ataagaaacc ttcctattct ctcttaagct ccattatatc atagaaactt aaggcaaact
     8761 aaaaaggaat gagaattttt tcttcccatt tccttctaaa atgttcatgt taagctgatt
     8821 cgaattgaga attatacaga tcagcataaa aaccactttg agacatcaaa acatcatgac
     8881 tgccttgctc aatgatattc ccatctttca tgaccaaaat caaatctgcg tttttaatcg
     8941 ttgataaacg atgtgcgata acaaatgatg ttcttccttc cataagtttg tccatagctt
     9001 tttgaattaa ctcttctgta cgtgtatcaa cagatgaagt tgcttcatct aagataagta
     9061 agggagaatt tttcaaaaga gctcgagcaa tggttaataa ttgcttttgt ccaactgaca
     9121 atgtcacagc gtcatcaagg acagtatcat accctttagg tagtgtcatt ataaaatgat
     9181 gtacaccgac tgccttagca gcttcttgaa cctcatcgtc actgattgtc gtctgattaa
     9241 aaatcaaatt ttctttaata gttccctcaa acaaccatat atcttgaaga accatcgaga
     9301 aagcatcatg aacttgacta cgtttcatgt cttggatatt gatgtcatct atggaaatac
     9361 tacctgaatt aacatcataa aaacgcatca ataaattaac aattgtcgtt ttacctgcgc
     9421 cagttggtcc tacaatagca actttctgac caggctgtgc ctccgaagta aagtcatgaa
     9481 taatagtctt gttatcacta taaccaaact tgacatgttc aaaggacaca taccccttaa
     9541 acttaggaag atttaacact ttgtgcttat cctcttgcat atttgtttca tttaaaaatt
     9601 caaaaacacg tcccatggct gcactagcag actgtaattg tgtaattcct tgagcaatct
     9661 gaccaatagg ctgtgtaaaa atacgaacat aggtcataaa agcaacaatt gtacccatag
     9721 taattgttcc tttgatagct aacatggcac caaccacaca caccatcaca taaccaaaat
     9781 taccaataaa ctgcatcaga ggcatcataa ttccagagaa aaattgtgat ttccacatgg
     9841 aactgtgaat tttatcattt aatagttgaa aatctgactt agcctgctgt cttgcgttat
     9901 gactagtaac aatgttatgt cctgagtaaa tttcttctac atatccagat atattagcca
     9961 aattcatctg ttgcttttta aataaaggct gactcttcac cataatgatt gaagaaagag
    10021 cgaatcctga aaataccgat ataatcgccg ttgttgctaa atgccaatct gttttgaaca
    10081 tcataattaa tgaaccaatc aataatactg tagatgcaac catagtaact aaactttggt
    10141 taaaagactg tgtcatcaaa tccacatcat ttgtaacacg agataaagta tcaccttgag
    10201 tatggctatc aaaataactc aaaggtattt tattgatttt atcagcaata gcattgcgca
    10261 tgccttgagc aaatttttga ataatagtcg ttacaataaa acttgctaga taggatacta
    10321 atgcacctaa aacatataat actgccaatt gccctgcaat atgaccgatt ttggctaagt
    10381 caatattacc agccaagcct tttaccataa gatccgtcat ctctttaaga cggtctggtc
    10441 ctataactgt tgtcaagctt gataaagttg ctaacacaat tgctaacgca aaaagaagtt
    10501 tgtaaccttt gaggtacgga gacaaattag agaaaaaaga cgttttttgt gatttagttt
    10561 tcattttcca attcctcctt agacaattga gaataggcta tctcttgata cacttcatta
    10621 tttgataaca attccttatg tgttccttgc ccaacaactt taccttgatc tagcactaaa
    10681 atgagatcag catccatgat agttgaaatg cgttgagcga caattaattt cgtcatagca
    10741 gctgtttttt ctttcagctc atggcgtaaa atatgatctg ttttataatc taacgctgaa
    10801 aatgagtcat caaaaatgag aatttcaggg tgacgtgcta aagcacgtgc aattgccaag
    10861 cgttgttttt gtcctcctga aaagttacta ccaccttgag cgacttctgt atctaatcct
    10921 ttttctttag cctcgataaa ttctttagct tgcgctaatt cgagtgcttg ccaaatttct
    10981 tcatcactaa gttcctccct gccactctct cccaaaatca tattagaacg tactgtccca
    11041 ctaaagagaa cagctttctg tggaatatag ccaattttat tattcaaatc attgtgagag
    11101 tattccttta tatcaatgcc atcaagttta attgccccct ctgtggcgtc ataaaaacgt
    11161 ggaactaaat tgactaaagt ggattttcca gaacctgtag acccaataaa tgccactgtc
    11221 tgaccagtat ctgctttgaa agatacatgt tcaataaccg cttcagaatc tttactataa
    11281 tgaaatgaca catcttgaaa ctcaatttga cctttttgtg cactttgaat atgactattt
    11341 tctttaaaag ttactgttgg ggctaaagtt agtacctcat taatacgtct aaccgatact
    11401 aaagcacgag gcatgatgat aaaaatagct accatcatca taaatcccat aacaacttgc
    11461 atagcataag atgaaaaaac aaccatgttt gaaaaaagat ccactcgatc agtgatagct
    11521 gatttagcag ccaatacaga tacacttgtt cctgtaactt taggcacaga gatatcatta
    11581 atcaaataag agccaatcca ataaatagct aaagtcaatc cagatgaaac cactgtcatc
    11641 acaggattca taagcgacat caatctataa acaaaaaggt tcaattttgt taaatcgtca
    11701 ttttcacgtt tgaacttatc tgtttgatac ttttcagcat tgtaggcacg aataactcta
    11761 acaccagaga gtgcctctct agtagttgca ttaagcgcat ctgtaaaacc ttggatttga
    11821 cgttgtctcg gaaaagcgag taagagtaga acgctaagta atatcataac gataataacc
    11881 gctactccaa ctgccccagt ccattgatcg cttttacccc aaattttagt caaagcccat
    11941 attgccataa ttggtccacg ggtaacaacc tgcattccca ttacaataag gatctgtagc
    12001 tgagtcaagt cgttagttgt gcgcgttaat agagagggga tagaaaatcg cttaatttct
    12061 gcatcagaat aatccatcac atggttaaaa atatctgaac gtaatcgact agtaaaacta
    12121 gctgctgtcc tagcacctaa aaaacctacc attactgcta ctaaaaaact accaaatgaa
    12181 aaggcaagca tttttagtcc aggatcccaa agagcctcta actttgtacc tttagtttgc
    12241 aacatacgtg tgatatcaga catatattct ggagtcttta ggtccatcca aaccgctaga
    12301 cacactaata tagtactcgc cacaatcatc aaccattctt taccattgag tcgcttgaaa
    12361 atattaaaca tcgcaaactc cttttacttt catatttatc ctattatcct aaaatagtta
    12421 atcaaaatct tccatcgcca tatttttagc taatgttcta ataacctttt tggtagtacg
    12481 tatctcttct ttagtaatac ctgctaacat ggtactgtgc aatttttcta agaaaatttc
    12541 aaattgtgta gccttttgtt tacctagatg agtcaaataa aggtatttca cacgcttatc
    12601 tgtcttagat ggaacaatcg caataaatcc atttttttcc atccgtttta ccaaattaga
    12661 agctacagac ttagagatgt gtaaaatttc ttcagcatct tttatagaca tatccttgtc
    12721 aggatgttta tagagatgca tcactaagtg tccttgtgga cccgctaaat gttcaacatc
    12781 atactctttt gcataacgat ctaaatactt ttcaagttga ttaacaagta ttcgtgcttt
    12841 ttgaagagga ttctccataa taatactcct ttaatagtta tcgtgagaac tattttaaaa
    12901 caacctttat taattgtcaa ttaaaagtta ccgtgagaac tatttttaca ttaatatttc
    12961 taaaaaaaac ctatcattcg tcaaataaaa aatgatttcg cttacttttt aatattaaaa
    13021 atatatgttc gctttttata tttttaatta taaatttata ttgattttta atgtaaatga
    13081 tagtaaaata caaatgtaat atttatttaa tttacaggag atttaaatga aaaagaaaat
    13141 tattttgaaa agtagtgtcc ttggtttagt cgctgggact tctattatgt tctcaagcgc
    13201 attcgctgac caagtcggcg tccaagttat aggcgtcaat gactttcatg gtgcacttga
    13261 caatactgga acagcaaata tgcctgacgg aaaagttact aatgctggca ctgctgctca
    13321 attagatgct tatatggatg atgctcaaaa agatttcaaa caaactaacc ctaatggtga
    13381 aagcattaga gttcaagctg gtgatatggt tggagcaagt ccagctaact cagggcttct
    13441 tcaagatgaa ccaaccgtta aaacatttaa tgcaatgaat gttgagtatg gcacattagg
    13501 taaccatgaa tttgatgaag gtttggcaga atacaatcgt atcgttactg gaaaggcccc
    13561 tgctccagat tctaatataa ataatattac gaaatcatac ccacacgaag ctgcaaaaca
    13621 agaaattgta gtggcaaacg ttattgataa agttaacaaa caaatccctt acaattggaa
    13681 accttacgct attaaaaata ttcctgtaaa taacaaaagt gtgaacgttg gctttatcgg
    13741 aatcgttacc aaagacatcc caaaccttgt cttacgtaaa aattatgaac aatatgaatt
    13801 tttagatgaa gctgaaacaa tcgttaaata cgccaaagaa ttacaagcta aaaatgtcaa
    13861 ggctattgta gtccttgctc atgtacctgc aacaagcaag gatgatattg ctgaaggtga
    13921 agcagcagaa atgatgaaaa aagtcaatca actcttccct gaaaatagcg tagatattgt
    13981 ctttgctgga cacaatcatc aatatacaaa tggtcttgtt ggtaaaactc gcattgtaca
    14041 agcgctctct caaggaaaag cctatgctga cgtacgtggt gtcctagata ctgatacaca
    14101 agatttcatt gaaacccctt cagctaaagt agttgcagtt gctcctggta aaaaaacagg
    14161 tagtgccgat attcaagcca ttgttgacca agctaatact atcgttaaac aagtaacaga
    14221 agctaaaatt ggtactgccg aggtaagtgg catgattacg cgttctgttg atcaagataa
    14281 tgttagtcca gtaggcagcc tcatcacaga ggctcaacta gcaattgctc gaaaaagctg
    14341 gccagatatc gattttgcca tgacaaataa tggtggcatt cgtgctgact tactcatcaa
    14401 accagatgga acaatcacct ggggagctgc acaagcagtt caaccttttg gtaatatctt
    14461 acaagtcgtc gaaattactg gtagagatct ttataaagca ctcaacgaac aatacgacca
    14521 aaaacaaaat ttcttccttc aaatagctgg tctgcgatac acttacacag ataataaaga
    14581 gggcggagaa gaaacaccat ttaaagttgt aaaagcttat aaatcaaatg gtgaagaaat
    14641 caatcctgat gcaaaataca aattagttat caatgacttt ttattcggtg gtggtgatgg
    14701 ctttgcaagc ttcagaaatg ccaaacttct aggagccatt aatcccgata cagaggtatt
    14761 tatggcctat atcactgatt tagaaaaagc tggtaaaaaa gtgagcgttc caaataataa
    14821 acctaaaatc tatgtcacta tgaagatggt taatgaaact attacacaaa atgatggtac
    14881 acatagcatt attaagaaac tttatttaga tcgacaagga aatattgtag cacaagagat
    14941 tgtatcagac actttaaacc aaacaaaatc aaaatctaca aaaatcaacc ctgtaactac
    15001 aattcacaaa aaacaattac accaatttac agctattaac cctatgagaa attatggcaa
    15061 accatcaaac tccactactg taaaatcaaa acaattacca aaaacaaact ctgaatatgg
    15121 acaatcattc cttatgtctg tctttggtgt tggacttata ggaattgctt taaatacaaa
    15181 gaaaaaacat atgaaataat aaaaataggt ttggatattt tatccaaacc tatttttata
    15241 tcaatatgcc ttccaaatga tctaactcgt gttgacaaat ttgagctgga aaatctgtca
    15301 acttgatttg ctgttctttc caattgatat cacgataaga aatcgtaatg tgacgatatc
    15361 gttgtgtact tctgcaacct acgagagaaa ggcaggattc ttctgtctca tatggatccg
    15421 attttgcaac taatacaggg ttaaacatta ctaaatttgt aatacccaca ttgatgataa
    15481 tgacacgctt cagactacca atcatatttg ctgccatacc tacacaataa ttttgattag
    15541 catgcaaagt atcctgtaaa tcttttgcta agctcacatc tgctctacta gccatttgtg
    15601 atttttgttg taaaaagaaa gtatctctaa caattggttt tatcatttat tttaacctaa
    15661 ctctattatg ataatactag tataccatag gttagctaag gactatgata agagattaaa
    15721 caataccttg tgcagacata gcttgagcaa cttttaaaaa gcctgcaatg ttagcaccta
    15781 gaaccaggtt accactatcg ccaaattcat cagtcgcttc ggaagcattc acaaaaatag
    15841 ttttcataat acgttgcaat tcttgatcaa cttcttcaaa agtccatgcc ctacgactag
    15901 aattttgtgc catttcaagc gctgaaacag ctactcctcc tgcattggca gctttagcag
    15961 gaccaaaagc aacccctgcc ttttggaaaa ctttaatagc acccaaggta gatggcatat
    16021 ttgctccttc tgttactgcc aagacaccat tttcaactaa taacttagcg tcttcttcgt
    16081 ctaattcgtt ttgagtagca cacgggaaag ctaaatctgc tttaatagac cagatactac
    16141 cttgatctgc tggtgtgaaa ttagcttttg gatgtttctc tgtatatttg acaatacgtg
    16201 ctctttcaac ctcttttatc tgtttcaaag tttcaaggtc aataccatct ggatcataaa
    16261 cataacctga agaatctgac acagctacaa cagttgcacc caactcttgt aatttttcag
    16321 tagcataaat agccacgtta ccagatcccg aaaccagtgc aactttgcca gttaaatctt
    16381 gaccacgcgc tgctagcatt tctttagcaa aatagactgc cccataacca gttgcttctg
    16441 tacgaaccaa agagccacca taagtcagac cttttccggt taaaacacca ttttgataac
    16501 catttaaacg cttatattga ccataaaggt aaccaatttc acgtccacct actccaatat
    16561 cgccagccgg cacatctaaa tctggtccaa tgtatttttg aagttcggtc ataaaacttt
    16621 gtgtgaaacg catcacttca ttatctgatt tcccttttgg atcaaagttc gaccctcctt
    16681 tgccaccacc aattggtaga cctgtcagtg aattcttaaa aatttgttca aatcctaaaa
    16741 atttgacaat agattgtgtt actgtagggt gaaaacgtaa accaccttta taaggaccaa
    16801 tagctgatga aaattgtaca cgatatcctc ggttaacttg aacctgacct ttatcatcta
    16861 cccatggaac tctaaaagaa atcacgcgct caggctcaac caaacgttct aaaagatttt
    16921 cttcaatgta ctgagggtat ctatcaaaaa ctggtactag tgactcaaaa acctcttcaa
    16981 ccgcttgtag aaattcttcc tcatgctcat tttgcttctt cactttctct aaaacacttg
    17041 ctacatatgt ctttccgctt gtcatttatt ttcctccgta ttgttttaat atagttagca
    17101 ttataaattg tcagaatttt cttgtcaact atttgctatg gtatcattct taggagcaat
    17161 tttaaaggta attctttgac ttttactgac tttcgttcta taatgagaca aatatttgat
    17221 tggagtatct atatgaaatg gttcccactt gttggaggta taattgcctt attagctggc
    17281 ttctatctct ttataaatcc tgtaacggtt gtagcaactc tgggttggtt cattgcttta
    17341 tttatcttta tttatggcat ttcaaattta gtacgctact tgcaagctga ttcttatcac
    17401 cgtagccctt ggtatctggt tcagagtatc cttgctatta tttttggtat cattttacta
    17461 tccagctcag caatgtcact ttcaaatact gtcatgacta ttttagcata ctggctactg
    17521 gtatctggta ttttacgact tttgggtggt ttccaaatgc aaaaaattaa tttcccaggt
    17581 gccaaatcct ataaacgttc ggcttatatt gcactcattt taggtattat tttactgatt
    17641 caacctacct tatcagcagc catcattgga agatttgtag cctttatatt aatagcaatt
    17701 ggagcttcgg ctatttacac ttttactcgt attaattatt aaaaggacat gtttcatgtc
    17761 ctttttttat ctattgatga cgtcatttac tatttgaaaa ttggctatta tataattcgt
    17821 aataaaatcc tttatctgct aggaggtgct ggtgattacc ttgttcgatg atttgaccat
    17881 cctttagaac taaaatgttg tctgcctctt gaatcgttga tagacggtgt gcaatgacaa
    17941 aactagttct tccttccatc aattttttca tagctttttg aataagcaat tccaaccttg
    18001 tatcaacaga tgaagtcgct tcatctaaaa ttaaaatttt aggatctgct aataaagcac
    18061 gtgcaatagt caacagttgc ttttgtccta aggagatatt gcttgattct tgattcatca
    18121 ccatattgta gccaccaggt aacgttcgaa taaagtggtc aacattcgca gctttggcag
    18181 cagcaactat atcctcatca ctggcttcaa gattaccgaa tctaagattt tcttttattg
    18241 taccttcata taaccaagca tcttgcaaca ccatgccaaa ttgttgccgg aaatcttggc
    18301 gtgataggtg tctaatatcg tgaccatcta cggtgatagc accttcacta acatcgtaga
    18361 atctcatgag taagtttata agtgttgtct tacctgcacc tgttggccct acaattgcaa
    18421 tcatttgccc tggttctacg tttaagttaa agtcgcggat aagtggctta tccggagaat
    18481 agtgaaaatc gacaccatga aagctgactt gaccggttaa gtcatggctc aatttttcag
    18541 tgacttgatt ggcttcctct tcttcatcta agacttcaaa aatacgttct aaggaagatt
    18601 ttgcactttg caaaacacct gctaattgtg taatggtttg aacgggttga ctaatttgcc
    18661 aaacatactg cacaaatgcc tgcatgttac caattgttaa ggtaccagca ataacttgta
    18721 aaccgccaac aaatgcaatg ataaggtaaa taaaatcaga gataccattc aatactggca
    18781 tcataattcc tgaaatgaag ctagctttga aacctacatg acgtagatta tccgttatat
    18841 cacgaaattc ttgactagac gcctcttctc taccatataa ttttatgata ttgaaaccag
    18901 ttaatttctc ttgtacaaaa ccattcagtt ctcccagaat ctttgcctgt tctttaaagt
    18961 aaggttggga tctttttagt atcgcttgtg cactaaaata agttacagga atacaagcaa
    19021 tgataatcat ggctaatggt acatttagat aaagtaccat aacgactact aaaataatac
    19081 ttaaaaaagc attgataatc tgtaaaaagc tctgttgtaa agcatttgag accgtttcaa
    19141 catcacttgt aaaacgtccc aacatatctc caaattggtg cttatca
//
LOCUS       DATRNK010000041        19109 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.041, whole genome shotgun sequence.
ACCESSION   DATRNK010000041 DATRNK010000000
VERSION     DATRNK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19109
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.041"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(56..1588)
                     /locus_tag="VBC52_001364"
     CDS             complement(56..1588)
                     /locus_tag="VBC52_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEO6882949.1"
                     /translation="MENHKNEFQFSLESILGFVWRGIVVGLISGFVVSIFRLAIEKIF
                     LVVMELYKSAHYQPIILLSITVTSIIAAVIIGFFIKSDPDIKGSGIPHVEGELKGMLS
                     PDWFSIVWKKFIAGILAISSGLMLGREGPSIQLGAMTGKGIAQYLNASRMEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLVWITALVASLVANFVSLNIFGLTPVLA
                     LPSELPSLNLNFYWIFLLMGLFLGILGFIYEWVILRFHVIYDYLGKLFHLPSHLYGIL
                     AVIFILPIGYYFPQLLGGGNGLIVSLPRSNLSLMMLGLFFLIRFLWSMLSYSSGLPGG
                     IFLPILALGSLAGAFFAVGMQYFGIISHQQISLFVVLGMAGYFGAISKAPLTAMILVT
                     EMVGDLKQLMAIGIVTMVSYIVMDLLKGEPIYEAMLAKMTFNPKDKVMTPTLIELTVS
                     DKISGKYVRDLELPENVLITTQIHHKTSAVVSGNTILNAGDTIFLVVNESEIKEVREQ
                     LM"
     gene            complement(1697..2770)
                     /locus_tag="VBC52_001365"
     CDS             complement(1697..2770)
                     /locus_tag="VBC52_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6882950.1"
                     /translation="MRRVYSFLGGIILVILILFGLTTYLEKKSSSTPNSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMHTKIKQGGTTYDIAVPSDYMIDKMIKEN
                     LLVKLDHSKIANWDAIGARFKNLSFDPKNKYSIPYFWGTVGIVYNDQLVKTPPKHWDD
                     LWRPEFRNKIMLVDSAREVIGVGLNSLGYGLNTKNISELKAASKKLDALTPNVKAIVA
                     DEMKGYMIQGDAAIGVTFSGEAREMLDGNKHLHYVVPSEGSNLWFDNIVIPKTVKHRK
                     EAYAFINFMMEPKNAAQNAEYIGYATPNLKAKALLPADIKNDKAFYPPDKTIDHLEVY
                     NNLGQKWLGIYNDLYLQFKMYRK"
     gene            complement(2763..3539)
                     /locus_tag="VBC52_001366"
     CDS             complement(2763..3539)
                     /locus_tag="VBC52_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6882951.1"
                     /translation="MKKFANIYLALVFIILYIPIIYLIFYSFNKGGDMNSFTGFTFSH
                     YGELFQDSRLMLILVQTFFLAFLSALLATIIGTFGAIWIYQVRRRHQTSILSLNNILL
                     VAPDVMIGASFLLVFTVIGLQLGFTSVLLSHVAFSIPIVVLMVLPRLKEMNDDMINAS
                     YDLGASTWQMLKEVMLPYLSSGIISGFFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARRGISLEINALSTIVFLFSILLVIGYYYISKEKGEKNA"
     gene            complement(3536..4330)
                     /locus_tag="VBC52_001367"
     CDS             complement(3536..4330)
                     /locus_tag="VBC52_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6882952.1"
                     /translation="MKKTSSLFSIPYMAWLFLFVLAPVALIAWNSFFDINGHFTLANY
                     QTFFSSGTYLKMSFNSVLYAGIVSFITLLISYPAAYLLTKLKHKQLWLMLVILPTWIN
                     LLLKAYAFMGIFGQQGGINAFLTFIGIGPKQILFTDFSFLFVAAYIELPFMLLPIFNA
                     LDDIDQNLIYASDDLGANAWQTFQKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLITQNKGMGSTIGVILILVMVAIMWLTKERKA"
     gene            complement(4314..5468)
                     /locus_tag="VBC52_001368"
     CDS             complement(4314..5468)
                     /locus_tag="VBC52_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6882953.1"
                     /translation="MTNPIIAFKNVSKVFEDSNTVVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLEASTGDIYLDGKRINDVPTNKRDVHTVFQNYALFPHMTVFENVAF
                     PLKLKKMDKKEIQKRVQETLKMVRLEGFEKRAIQKLSGGQRQRVAIARAIINQPKVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILSGKMIEDYLVEFNGKRFEAVDGGMRPNESVQV
                     VIRPEDLQITLPDEGKLQVKVDTQLFRGVHYEIIAYDDLGNEWMIHSTRKAIEGEVIG
                     LDFTPEDIHIMRLNETEEEFDARIEEYVDTDDHEDGLINAIEEERNEENL"
     gene            complement(5517..6419)
                     /gene="murB"
                     /locus_tag="VBC52_001369"
     CDS             complement(5517..6419)
                     /gene="murB"
                     /locus_tag="VBC52_001369"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEO6882954.1"
                     /translation="MIKTIQKELEGLDIRFDEPLKKYTYTKVGGPADYLAFPRNRLEL
                     SRIVKFANSQNIPWMVLGNASNIIVRDGGIRGFVIMFDKLSTVTVNGYVIEAEAGANL
                     IETTRIARYHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILLSAQVLTPQGELKT
                     IEARNMQFGYRHSVIQESGDIVISAKFALKPGDHLMITQEMDRLTYLRELKQPLEYPS
                     CGSVFKRPPGHFAGQLISEAHLKGQRIGGVEVSQKHAGFMVNIAEGSAQDYENLIEHV
                     INTVESTSGVHLEPEVRIIGESLL"
     gene            complement(6563..7051)
                     /gene="folK"
                     /locus_tag="VBC52_001370"
     CDS             complement(6563..7051)
                     /gene="folK"
                     /locus_tag="VBC52_001370"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEO6882955.1"
                     /translation="MTTVYLSLGSNIGDRETFLKQALFSIDHLQKTKVAQISAIYETA
                     AWGNTNQEDFFNICCQVETDLAPFELLDYCQEIEKRLKRVRHEHWGPRTIDIDILLFG
                     NQVINQEDLVVPHPYMTKRAFVLVPLLEIAPQLSLPNGSKLEDYLEKLNLGEVHYFKP
                     VE"
     gene            complement(7048..7410)
                     /gene="folB"
                     /locus_tag="VBC52_001371"
     CDS             complement(7048..7410)
                     /gene="folB"
                     /locus_tag="VBC52_001371"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEO6882956.1"
                     /translation="MDKIYLNKCRFYGYHGAFSEEQTLGQVFQVDAVLSLDLAKASQT
                     DDLIDTVHYGEVFDCIKNHVENEQYQLIEKLAGVIVEDIFLQFHPVQAITLKITKDNP
                     PINGHYESVGIELERKRA"
     gene            complement(7412..8215)
                     /gene="folP"
                     /locus_tag="VBC52_001372"
     CDS             complement(7412..8215)
                     /gene="folP"
                     /locus_tag="VBC52_001372"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEO6882957.1"
                     /translation="MKIGQYDITGKACIMGILNVTPDSFSDGGSYTTIDSALNQVGEM
                     LEQGVAIVDIGGESTRPGAVFVTAEEEIKRVVPMIKAIREVYPDLLLSIDTYKTEVAQ
                     AALDAGVHILNDVWSGLYDGKMLSLAAERNVPIILMHNQEEAVYQDIKKEVCEFLLER
                     AERALEAGVSKDNIWIDPGFGFAKTEEQNLELLKGLEQVCDLGYPVLFGISRKRTVNY
                     LLGGNREVTERDMGTAALSAWAIAKGCQIVRVHNVEVNKDIVTVISQLV"
     gene            complement(8219..8782)
                     /gene="folE"
                     /locus_tag="VBC52_001373"
     CDS             complement(8219..8782)
                     /gene="folE"
                     /locus_tag="VBC52_001373"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEO6882958.1"
                     /translation="MPNQEKMEKAIYQFLEALGENPNREGLKDTPKRVAKMYIEMFSG
                     LNQDPKEQFTAVFSENHEEVVIVKDIPFYSMCEHHLVPFYGKAHIAYLPNDGRVTGLS
                     KLARAVEVASKRPQLQERLTAQVAQALEDALAPKGIFVMIEAEHMCMTMRGIKKPGSK
                     TITTVARGLYKDDRYERQEILSLIQKG"
     gene            complement(8801..10063)
                     /locus_tag="VBC52_001374"
     CDS             complement(8801..10063)
                     /locus_tag="VBC52_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEO6882959.1"
                     /translation="MTYQEALEWIHSKLAFGIKPGLERMRWMLEQLGNPQNNLSAIHV
                     VGTNGKGSTTSYLQHIFTNSGYQVGTFTSPYIVDFRERISIDGQMIPESDFIKLVETV
                     RPVVERLHLETNLEPATEFEVITVLMFYYFGNSCPVDIVIIEAGMGGYYDSTNMFKAL
                     AVTCPSIGLDHQEVLGRTYVDIAEQKVGVLKKGVPFVYANDRQDVEEVFQIKAKETHS
                     QTYRLHNDFYIKEEENYFNYIGPQANIDHIQLQMPGHHQVSNASIAITTSLLLRDKYP
                     KLTLQTIKDGLEMTKWVGRTELIFPNVMIDGAHNNESVDALVQVIKKYQQKNVHILFA
                     AINTKPIESMLESLSSIAPVSVTSFDYPKSISLDKYPKAYTRVSDWKKWLHDINLTSD
                     KDFYVITGSLYFISQVRRELLLIKTTTT"
     gene            complement(10065..10952)
                     /gene="rarD"
                     /locus_tag="VBC52_001375"
     CDS             complement(10065..10952)
                     /gene="rarD"
                     /locus_tag="VBC52_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEO6882960.1"
                     /translation="MRKDNLGIILGLSAYVLWGLLSLYWKLLSGIEAYSTFAYRIIFT
                     VLTMLIYMLVSGRKTVYLKDLKGLVNNKKSFWTMFVASILISINWLVYIFAVTHGHAT
                     EASLGYYMMPIISILLSVLVLREHLARVVSLAILIAIMGVGVLVYQTGHFPLISLTLA
                     LSFGFYGLLKKSISLSSDFSMLVESSFIAPFALIYIVFFAKDFLTDYNILQLVLLSLS
                     GIITAVPLLLFAEAIKRAPLNIIGFIQYINPTIQLLLALFIFKETIVSGEIIGFIFIW
                     LAILVFSIGQVHTMLKKGK"
     gene            complement(10939..11805)
                     /locus_tag="VBC52_001376"
     CDS             complement(10939..11805)
                     /locus_tag="VBC52_001376"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEO6882961.1"
                     /translation="MRIIVPATSANIGPGFDSIGVALSKYLIIEVLEESTEWLVEHNL
                     VNIPKDHTNLLIQTALHVKSDLAPHRLKMFSDIPLARGLGSSSSVIVAGIELANQLGN
                     LALSQKEKLEIATRLEGHPDNVAPAIFGDLVISSIVKNDIKSLEVMFPDSSFIAFIPN
                     YELKTSDSRNVLPQKLSYEDAVASSSVANVMVASLLKGDLVTAGWAIERDLFHERYRQ
                     PLVKEFEVIKQISTQNGAYATYLSGAGPTVMVLCSKEKEQPIVTELSKLCLDGQIQVL
                     NIERKGVRVEKR"
     gene            complement(11807..13090)
                     /locus_tag="VBC52_001377"
     CDS             complement(11807..13090)
                     /locus_tag="VBC52_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEO6882962.1"
                     /translation="MTIKIALLGFGTVAKGIPYLLKENQHKLLSLEGEDIVIDKVLVR
                     DNESRQRFINQGFTYNFVTEINTILQDPQIDIVVELMGGIEPAKTYLSQALGFGKHIV
                     TANKDLIALHGKELMDLADARGLALFYEGAVAGGIPILRTLSHSFASDKMTRLLGILN
                     GTSNFMLTKMFEEGWSYEQALKKAQELGYAESDPTNDVEGIDTAYKATILSQFGFGMP
                     IDFDDVNYKGISSIRSEDVEVAQEMGFAIKLVADLRETPTGISVDVSPTLISQKHPLA
                     AVNHVMNAVFIESIGIGQSLFYGPGAGQNPTATSVLADIIDISRSIRSQIKIKPMNTY
                     HCPCRLSMQSDIFNEYYLAISLRNAEDSDTLGRYFEQENIGLKNVIEKALGDKQQEIY
                     VLTDEVSQEKITQFIEEFPESGVIQLINVFKVIGG"
     gene            13267..14154
                     /locus_tag="VBC52_001378"
     CDS             13267..14154
                     /locus_tag="VBC52_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO6882963.1"
                     /translation="MAHTPTSHRKPRKRSPWLAIASVFFLLIALIGIFLFFNNRSKQE
                     IKTKTNASSHRKIVTSIKKKKWIKQKTPVKIPILMYHAVHVMDPSEAASANLIVAPDI
                     FESHIKRLKKEGYYFLAPNEAYRALNENALPDKKVIWITFDDGNADFYTKAYPILKKY
                     KVKATNNIITGFVQEGRESNLNVQQMLEMKQNGMSFQGHTVTHPNLSLLTPELQTQEM
                     TLSKQFLDQKLSQDTLAIAYPSGRYNPTTLDIASQYYKLGLTTNEGVATKDNGLLSLN
                     RVRILPTTSDDDLIKTINQ"
     gene            complement(14177..15724)
                     /locus_tag="VBC52_001379"
     CDS             complement(14177..15724)
                     /locus_tag="VBC52_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEO6882964.1"
                     /translation="MSKKHITPVFTGSLIVSLILVLLGIIVPRGFQSWTQILREQVST
                     NFGWLYLLLVTSILALCVFFIMSPLGQIRLGQPHSRPEYSTVSWIAMMFSAGMGIGLV
                     FYGAAEPLSHFAISTPGAPKESQTALADAFRFTFFHWGIHAWAVYALVALALAYFGFR
                     KQEKYLLSVTLKPLFGDKTDGWLGKIVDITTVVATVIGVATTLGFGAAQINGGLSFLL
                     GVPNNAFVQIVIILITTALFVMSALSGLGKGVKILSNLNLILAVALLALVIVLGPTVR
                     IFDTLTESLGSYLQNFFGMSFRAAAFDNTKRSWIDNWTIFYWAWWISWSPFVGVFIAR
                     ISKGRSIREFLTVVLLIPTLLSFVWFAAFGTLSTQVQQLGTNLTKFATEEVLFATFNH
                     YTLGWLLSIIAIILIFSFFITSADSATYVLAMLTEDGNLNPKNRTKVIWGLVLAVIAI
                     VLLLSGGLLALQNVLIIVALPFSFVMILMMLALLVELFHEKKEMGLSISPDRYPRKNE
                     PFKSYEE"
     gene            complement(15867..17243)
                     /locus_tag="VBC52_001380"
     CDS             complement(15867..17243)
                     /locus_tag="VBC52_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEO6882965.1"
                     /translation="MAYKTIYPYTNEVLHEFDNISDSDLEQSLDIAHALYKTWRKEDN
                     VEERQNQLHKVADLLRKDRDKYAEVMTKDMGKLFTEAQGEVDLCADIADYYADNGQKF
                     LKPVPLESPNGEAYYLKQAVGVLLAVEPWNFPFYQIMRVFAPNFIVGNTMLLKHASIC
                     PASAQAFEDLVREAGAPEGAFKNIFASYDQVSNLISDPRVAGVCLTGSERGGASIAAE
                     AGKNLKKSSMELGGNDAFLILDDADFDLLSKTIFFARLYNAGQVCTSSKRFIVMADKY
                     DEFVNMVVETFKSAKWGDPMDSETTLAPLSSAGAKDDVLKQIKLAVDHGAEVVFGNDT
                     IDHPGNFVMPTVLTNITKANPIYNQEIFGPVASIYKVDTEEEAIALANDSSYGLGSTV
                     FSSDPEHAKKVAAQIETGMTFINSGWTSLPELPFGGIKNSGYGRELSQLGFDAFVNEH
                     LVFTPNSD"
     gene            complement(17317..18324)
                     /locus_tag="VBC52_001381"
     CDS             complement(17317..18324)
                     /locus_tag="VBC52_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEO6882966.1"
                     /translation="MLFKEKIPGLILCFIIAIPSWLLGLYLPLIGAPVFAILIGIIVG
                     SFYQNRQLFNKGIAFTSKYILQTAVVLLGFGLNLMQVMKVGISSLPIIIMTISISLII
                     AYVLQKLFKLDKTIATLIGVGSSICGGSAIAATAPVINAKDDEVAQAISVIFLFNILA
                     ALIFPTLGNFIGLSDHGFALFAGTAVNDTSSVTATATAWDAINHSNTLGGATIVKLTR
                     TLAIIPITIVLSIYHMKQTQKEQSVSVTKIFPKFVLYFILASLLTTIVASLGFSLRIF
                     EPLKVLSKFFIVMAMGAIGINTNVSKLIKTGGKSILLGAACWLGIIIVSLTMQAILGT
                     W"
     gene            complement(18432..>19109)
                     /locus_tag="VBC52_001382"
     CDS             complement(18432..>19109)
                     /locus_tag="VBC52_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882967.1"
                     /translation="FLKVFLTVLVLILIVGAGGLYFWNNHQSLEGKWRTVSLEKQVEK
                     EIEQQLGSQAADMGISAADLVKGANMHMNVKNDEAKITVTAQIDEVKFHQAIKTFIDK
                     ALEKQLKDQGLTYNDLSEAGKKIFDETKITDQQIDQQIDRSFQSAAQAAGGKYNTNTG
                     EMTLPVMDGKVHRLTSVIKVSHINKKANAFYGNIVKNGEKTAYKKEGSKLILGNEKSY
                     PFMKVTK"
BASE COUNT         6461 a         3876 c         2878 g         5894 t
ORIGIN      
        1 aactagttaa atccatgact agtttctatt ttttattatt taagcgttaa ttagtttaca
       61 tcagctgctc acgtacttct ttaatttccg attcattcac aactaaaaaa atggtgtcac
      121 cagcattcaa gatagtattt cctgatacta cagccgatgt cttatgatga atttgagtcg
      181 ttattagcac attttctggt aattctaaat ctcggacata ctttcctgat attttatctg
      241 aaacagttaa ttcaatcaat gtaggtgtca ttactttatc ttttggattg aaggtcattt
      301 tagccaacat cgcttcataa ataggttctc cctttaataa atccatgaca atataagata
      361 ccattgttac aattccaata gccattaatt gttttaaatc gccaaccatt tctgtaacca
      421 aaatcatagc tgttagtggt gcttttgaga ttgcaccaaa atagcccgcc attcctagaa
      481 caacaaataa agatatctgc tgatgagata ttataccaaa gtactgcata cctactgcaa
      541 aaaaagcacc tgctaaagat cctaaagcta gaattggtaa aaaaattcct cctggcaagc
      601 cactactgta actcaacata ctccataaaa aacgaatgag gaaaaataat cctaacatca
      661 tcagactaag attactccta ggcagtgata caattaagcc atttccaccc cccaataatt
      721 gtgggaaata gtaaccaata ggaagaataa aaattaccgc taatatacca tataggtgag
      781 atggtagatg aaatagttta cctaaataat catagatcac atgaaatctc aaaattaccc
      841 actcatagat aaagcctaag atacccaaaa agagacccat gagtaaaaaa atccaataaa
      901 agttgagatt taaagatggt aattctgatg gtaaagctaa aactggggtt aaaccaaaga
      961 tattaagtga gacaaagttt gccactaaac ttgctaccaa agctgttatc caaactaagc
     1021 gagaaaaatg atggtaaatt tcttcaacta caaataaaag gcctgcaata ggagcattga
     1081 aggctgctga aagacctgct gctgcaccac tagcaattaa aacacgtttt tccattcgac
     1141 tagcattgag atactgtgca attcccttac ctgtcatagc tcctaactga atactgggac
     1201 cttcgcgacc aagcataagt cccgaagaaa tagctaaaat acccgcaatg aatttcttcc
     1261 atacaatact aaaccagtct ggcgatagca ttcctttaag ttcaccttca acatgcggga
     1321 tacctgaacc tttaatatca ggatcagatt tgataaaaaa acctattatc actgcagcga
     1381 taatactagt aaccgtaatg ctcaataaaa taattggttg ataatgggca cttttataaa
     1441 gttccataac aactaaaaaa attttttcaa tcgctaatcg aaatatgctg acaacaaatc
     1501 ctgatattaa gcctactact attcctctcc aaacaaatcc cagaattgat tcaagtgaaa
     1561 actgaaattc atttttatga ttttccatat tttcctcctt ttcttacaat tattttaaca
     1621 aaaatatcac aaactttcta ggaactcttg gtaactaaaa atacctctca tcagagaagt
     1681 acttttagtt aatagattac ttacgataca ttttaaattg taagtacaaa tcattataaa
     1741 taccaagcca tttttgaccc aagttattat aaacttctaa atgatcaatt gttttatcag
     1801 gaggataaaa tgctttatca tttttgatat ctgctggtaa cagcgctttt gctttgagat
     1861 taggggtcgc ataaccaata tattctgcat tttgtgccgc atttttaggt tccatcatga
     1921 aattaataaa ggcataagct tctttgcgat gctttactgt tttggggatt acaatattat
     1981 caaaccaaag gttcgatcct tcactaggaa caacgtaatg caaatgcttg ttcccatcaa
     2041 gcatttctcg tgcttctcca gagaatgtaa caccaatcgc tgcatctccc tgaatcatat
     2101 agcctttcat ctcatcagca acaatagcct taacgtttgg tgttagagca tctaactttt
     2161 tagaagcggc ttttaattct gatatgttct ttgtattcaa tccatatccc aaggaattta
     2221 aaccaactcc aataacctca cgtgcactat caactagcat gattttattt cgaaactctg
     2281 gacgccataa atcatcccaa tgtttaggag gagttttaac taattgatca ttataaacaa
     2341 tcccaacagt tccccaaaaa taaggaatcg aatatttatt tttaggatcg aaactcaaat
     2401 ttttaaacct agctccaata gcatcccaat tagctatctt tgaatgatca agtttaacca
     2461 gaaggttttc ttttatcatt ttgtcaatca tataatcact aggtacagca atatcatatg
     2521 tcgtcccacc ctgcttaatt ttggtgtgca tagcctcatt agagtcaaac gtttcgtatt
     2581 gtacttcaat tccagtttct tttgtaaatt tttttaataa ggctggatca atatagtctc
     2641 cccagttata aataactaac ttatctgagt taggtgttga actactcttc ttttctaagt
     2701 aagtggttaa accaaagagg atcaggataa caagaataat accgcctaaa aaggaataaa
     2761 ctctacgcat tcttttctcc cttctctttc gaaatatagt aataaccaat taccaataaa
     2821 atactaaata aaaagacaat agttgacaag gcattaattt ccaatgatat accgcgacgt
     2881 gcgcgtgaat agatttctac tgaaagcgtt gaaaagccat tacctgtaac aaagaaagta
     2941 accgcaaaat catctagcga gtaagtgaaa gccataaaga aacctgaaat aatacccgac
     3001 gacaaatagg gtaacataac ttctttaagc atttgccaag ttgaagctcc taaatcatag
     3061 gaagcattaa tcatatcatc attcatttct tttaaacgag gtaagaccat taaaacaaca
     3121 ataggaatag agaatgcaac atggctcaat aatacagaag tgaaacctaa ctgtaaacca
     3181 ataacagtaa atactaagag aaaacttgca ccaatcataa catctggtgc tactaaaagg
     3241 atattattaa gcgataaaat agaggtttga tgtctacgtc ttacctgata aatccaaata
     3301 gctccaaagg taccaataat tgtagctaat aaggcgctaa gaaaagccaa aaagaatgtt
     3361 tgcactaaaa ttagcattaa gcggctatcc tgaaacagtt ctccatagtg actaaaggtg
     3421 aatccagtaa aagaattcat atccccacct ttattgaaag aataaaaaat caaataaata
     3481 ataggaatat aaaggataat aaaaacaagt gctaaataga tatttgcgaa ttttttcatg
     3541 ctttcctctc ctttgttaac cacataatag caaccatcac taaaattaaa ataaccccta
     3601 tggttgagcc cataccttta ttttgtgtga ttaaaaaatg ttgttctatg gctgtcccta
     3661 aagtaataac tcgattacca ccaataagac gcgtaagcat aaagagactt aaactaggaa
     3721 taaagacaga ttgtacacct gctcttacac cattcagaga tagaggaaat ataaccttct
     3781 gaaaagtttg ccatgcattt gcacccaagt catcacttgc ataaatgaga ttttgatcaa
     3841 tatcatccaa ggcattaaaa atgggtaata acataaacgg taattcaatg taagctgcta
     3901 caaaaagaaa tgaaaaatct gtaaatagaa tctgttttgg gccgatacct ataaaggtta
     3961 agaaggcgtt aattccccct tgttgaccaa atatccccat aaaagcatag gcttttaaca
     4021 aaagattaat ccatgttggc aaaataacta gcattagcca taattgcttg tgttttaatt
     4081 ttgtaagcaa ataagcagct ggatagctaa tcaaaagcgt tatgaaacta acgatgcctg
     4141 catataggac cgagttaaag ctcattttaa gataggttcc tgagctaaaa aacgtctggt
     4201 aattagctaa agtaaagtgc ccattaatat caaagaacga gttccaagca atcaaagcaa
     4261 caggtgctaa tacaaataag aataaccaag ccatataagg aattgaaaat agactagagg
     4321 ttttcttcat ttctctcctc ctcgatagca ttgattaaac catcttcatg atcgtcagtg
     4381 tctacatatt cttcaatgcg cgcatcaaat tcttcttctg tttcattaag acgcataata
     4441 tgtatatctt ctggagtgaa atctaaccca ataacttcac cctcaatagc tttacgagtt
     4501 gaatgaatca tccattcatt acctaaatca tcataagcaa taatttcata gtggacacct
     4561 cgaaaaagtt gtgtgtccac tttaacttga agttttcctt catcgggtag ggtaatttga
     4621 agatcttctg gacgaataac tacttggact gattcattag ggcgcatacc gccatcaaca
     4681 gcctcaaagc gcttaccgtt aaattcaacc aaataatcct caatcatctt gccagataag
     4741 atatttgatt cacctataaa agttgctaca aaatgattga taggttcatc gtagatatct
     4801 actggtgttc ctgattgaac aatttcacct tcattcatga cgaaaatcca atcactcatt
     4861 gccaacgctt cttcttgatc atgtgtgaca aaaacaaagg taattcctaa acgttgttgc
     4921 agttcgcgca attcatattg catctctgta cgcaatttca aatccaatgc tgataagggt
     4981 tcatctagta agactacttt aggttgattg atgatagcac gcgctatcgc aacacgctgg
     5041 cgttgtcctc ctgataattt ttgaatagct cgcttttcaa acccttctaa acgaaccatt
     5101 tttagagttt cttgaacacg tttttgaatt tctttcttat ccattttttt caactttaat
     5161 ggaaaagcaa cgttttcaaa aaccgtcata tgtggaaaca aagcatagtt ttggaaaacg
     5221 gtatgaacat cccgtttatt agtgggaaca tcattgatgc gtttcccatc taagtaaata
     5281 tcgcctgtac tcgcctctag caaacctgca ataatattta agatagttga tttaccactt
     5341 cctgaggccc ctaataaggt gtaaaattta ccttcttcaa gttcaaaatt aatatctttt
     5401 aagacaaccg tgttgctgtc ttcgaatacc tttgatacat ttttaaatgc aatgattggg
     5461 ttagtcaatt ctcataaatc ctccattttt ctgactaaag taagtactaa actactttat
     5521 aatagcgact caccgataat acgaacttct ggttccaaat ggacacctga agttgattca
     5581 acggtgttaa taacatgttc aataagattt tcataatctt gagctgaacc ttctgcaatg
     5641 ttaaccataa aaccagcatg tttttgacta acctctactc caccaatacg ttgaccttta
     5701 agatgtgctt cgctaatgag ttgccctgca aaatgtcctg gaggacgttt gaacacagaa
     5761 ccacaagaag ggtattctaa aggttgtttc agctcacgga gataggttaa acgatccatt
     5821 tcctgagtaa tcatgagatg atctcctggt tttaaggcaa acttagctga tataacaata
     5881 tctccactct cttgaatgac agaatgacga taaccaaatt gcatattcct agcttcaatc
     5941 gttttgagtt caccctgtgg tgtcaaaact tgtgcagaca ataaaatatg agcaatttca
     6001 ccaccgtaag cgccagcatt cataaagact gctccaccta cacttccagg aattccgcaa
     6061 gcaaactcaa agccagttaa actatgataa cgtgcaatac gagttgtttc aatgagatta
     6121 gctcctgctt ctgcttcaat aacataaccg ttgactgtaa cagttgacag tttatcaaac
     6181 atgataacaa aaccacggat accaccatct ctaacgataa tattactcgc atttcctaaa
     6241 accatccaag gaatattttg actgttagca aacttaacaa tacgcgatag ttcaaggcga
     6301 ttccttggga atgcaagata atcagcaggt cctcctactt tagtataagt atatttcttt
     6361 aaaggctcat caaagcgaat atcgagacct tctaattctt tttgtattgt tttaatcata
     6421 acctttatct caattctgtt ttattgtgtt ttatagacac aaaaaatcgg cataaaaccg
     6481 atttctatta taccaaaaat ataggttgtt gacgatacat tatcacaatt tctgtgtatt
     6541 ttaattttaa aatcctaact gactattcga ctggcttgaa ataatgaact tctcctaaat
     6601 tcaatttttc gagatagtct tctagcttag aaccattagg taaacttagt tgaggtgcta
     6661 tttcgagtaa cggaactaaa acaaatgctc gcttcgtcat atacggatgg ggtactacta
     6721 aatcttcttg gttaatcact tgattaccaa aaagtaagat atcaatatct attgtccttg
     6781 gaccccagtg ctcatgtctc acgcgcttta agcgtttttc gatttcttga caataatcca
     6841 ataattcaaa aggtgctaag tctgtctcta cctgacaaca tatattaaaa aaatcttctt
     6901 ggttagtatt cccccaagca gctgtttcat aaatagcgga aatttgcgcg acttttgtct
     6961 tttgcaaatg atcaattgaa aagagagcct gcttcagaaa agtttctcta tcaccaatat
     7021 tactacctaa actcaagtaa acggttgtca tgctcgcttg cgctccaatt caattccaac
     7081 tgattcatag tgcccattaa ttggtggatt gtcttttgta atctttaatg taatagcttg
     7141 tactggatga aactgtaaga aaatatcttc aacaataaca cctgctaatt tctctattaa
     7201 ttgatactgt tcgttttcaa catgattttt aatacagtca aaaacttcac cataatgaac
     7261 cgtatcaatt aaatcatcag tttgtgatgc ttttgccaaa tcaagtgata gaacagcatc
     7321 aacttgaaaa acttgcccca atgtttgttc ttccgaaaaa gcaccatgat aaccataaaa
     7381 ccgacattta tttaaataaa tcttatccat attacaccaa ctgactaatt actgtcacaa
     7441 tgtccttatt tacttcaaca ttgtgaacac gtacaatctg acaccccttt gctattgccc
     7501 atgccgataa agcagcagtc cccatatcac gttcagttac ttccctatta ccacctagaa
     7561 gataattaac agtacgtttt cgagagatcc caaataacac aggataacct aaatcacaca
     7621 cttgttccaa accttttaat agttctaaat tttgctcctc tgttttagca aaaccaaagc
     7681 caggatctat ccaaatatta tcctttgaaa cacctgcttc taaagcacgc tctgctcttt
     7741 caagtaagaa ttcacaaact tcttttttga tatcttgata aacagcttct tcctggttat
     7801 gcatcaaaat aattggaaca tttcgttctg ctgctaaaga tagcatttta ccatcatata
     7861 aacctgacca aacgtcattt aaaatatgta cacctgcatc aagggctgct tgagctacct
     7921 ctgttttata agtatctatt gataacaaaa gatcagggta gacctctcta atagctttaa
     7981 tcattggaac tacacgttta atttcttctt cagcagtcac aaatactgca cctggccgcg
     8041 ttgattctcc cccaatatct acaattgcca ccccttgttc aagcatctca ccaacttgat
     8101 ttaaagctga atcaattgtg gtataagaac caccatctga gaaggaatca ggcgttacat
     8161 ttaaaatccc catgatacaa gctttacctg taatatcata ttgtcctatt ttcatctttt
     8221 atcctttttg tattagcgat agaatttctt gtctttcata tcgatcgtcc ttgtataaac
     8281 cacgagctac cgttgtaatc gtcttacttc ctggtttttt aatgccacgc attgtcatgc
     8341 acatatgctc tgcttctatc ataacaaaaa tacctttagg cgccaaagca tcctctaaag
     8401 cttgtgcaac ttgtgctgtt aaacgttcct gaagttgagg acgcttactc gccacttcaa
     8461 ctgcacgtgc aagcttacta agtccagtaa cacgtccatc gttcggtaaa taagcaatat
     8521 gagctttacc ataaaaagga accaaatgat gttcacacat ggaatagaat ggaatatcct
     8581 tgacaatcac aacctcttca tgattttcag aaaagactgc agtgaattgc tcctttggat
     8641 cttgattaag tcctgagaac atttctatat acatttttgc cacacgcttc ggtgtatctt
     8701 tcaatccttc tcgattagga ttttctccta aagcctctaa aaattgataa atagcttttt
     8761 ccattttttc ttgattgggc attaatttat cctatccttt ttaagttgtt gtagttttga
     8821 ttaatagtag ttcccgacga acttgagaaa taaaataaag ggagccagta atcacataga
     8881 aatctttatc tgatgtcaga tttatatcat gtagccattt cttccaatca ctaactctag
     8941 tgtacgcttt aggatatttg tctaggctta ttgatttagg gtaatcaaag ctagtaaccg
     9001 aaaccggagc gatactagac aatgattcta acatactctc tatcggttta gtattaattg
     9061 cagcaaatag aatatgcacg tttttctgct gatatttttt tataacttga acgagagcat
     9121 cgacactttc attattatga gcaccatcaa tcattacatt tggaaagatt aattctgtac
     9181 ggcctaccca ttttgtcatt tcaagcccat ctttaatggt ctgtagggtt aactttggat
     9241 atttgtctct taataataaa ctggtagtta tagctatgct agcattggaa acttggtgat
     9301 gtcctggcat ttgcaactga atatgatcaa tatttgcttg aggacctata tagttaaaat
     9361 aattttcctc ttcttttata taaaagtcat tgtgtaaacg ataagtctgg ctgtgtgttt
     9421 ccttggcttt tatttgaaag acttcttcaa catcctgacg atcattagca tatacgaatg
     9481 gaactccttt ttttaaaaca cctacttttt gctcagctat atctacataa gttcgtccta
     9541 aaacctcctg atgatctaat ccaattgacg gacaagtaac cgctaaagcc ttgaacatgt
     9601 tggtagaatc ataataaccg cccattccag cctcaataat gacgatatca accggacagc
     9661 tattgccaaa gtaatagaac atcaaaactg ttataacttc aaattctgta gctggttcta
     9721 ggttggtctc aaggtgcaac cgttcaacaa ctggacgaac agtttcaaca agtttgataa
     9781 aatcagactc tggaatcatt tgaccatcaa tgctaatacg ctctctaaag tcaacaatat
     9841 acggtgatgt aaatgttcca acttgataac cagaattggt aaaaatatgt tgtaaataac
     9901 tagttgttga tcctttacca ttcgttccaa caacatgaat ggcagatagg ttgttttgag
     9961 ggtttcccaa ctgctctagc atccatctca tacgttccaa acctggttta ataccaaaag
    10021 ctaatttgct atgaatccat tctagcgctt cttgataagt catcttattt acctttcttt
    10081 aacatcgtat gtacttgtcc aatactaaaa actaaaatag caagccagat aaaaataaaa
    10141 cctattatct ccccactaac gatagtttct ttaaaaataa acaatgctag caataattgg
    10201 atggttggat tgatgtattg gataaaaccg ataatattta gaggagccct cttaatggct
    10261 tctgcaaata ataaaagagg aactgctgta atgattcctg aaagtgagag taaaactaat
    10321 tgtaatatat tataatcagt tagaaaatct ttggcgaaaa acactatata aatgagtgca
    10381 aacggagcaa taaaactact ttctaccagc atcgaaaaat cgctagataa agaaattgat
    10441 ttttttaata acccataaaa accaaaagaa agcgctagag ttagtgaaat taaaggaaaa
    10501 tgaccagttt gataaactaa aactccaact cccatgattg caatcaaaat tgcaaggcta
    10561 actactcttg ccagatgttc acgtaaaaca aggaccgaca acaaaattga aataatcggc
    10621 atcatataat aaccaagact tgcctcagta gcgtgaccat gcgtgactgc aaaaatatag
    10681 accaaccaat taattgaaat taaaatactg gctacaaaca ttgtccaaaa tgacttttta
    10741 ttgttaacta agccttttaa gtcttttaaa tagactgttt ttctacccga aacgagcata
    10801 taaataagca tagttaaaac agtgaatata atcctataag caaacgtact atatgcttca
    10861 attccagata ataatttcca ataaagagat aacagccccc ataaaacata ggctgacaag
    10921 ccaaggataa ttcctaaatt atcttttctc aactctcact ccttttcgct caatattaag
    10981 cacttgaatc tgtccatcta aacataattt acttaactct gtgacaattg gttgttcttt
    11041 ttctttcgaa cacagaacca ttaccgtcgg tcctgctcca gacaaataag tagcatacgc
    11101 accattttga gtgctgattt gttttataac ttcaaattcc ttcaccaaag gttgacgata
    11161 tctttcatga aagaggtccc tttctatagc ccatccagca gttaccaaat cacctttcaa
    11221 caacgacgca accatcacat tagcgacgct gcttgaagca actgcatcct cataactcaa
    11281 tttttgaggt agtacatttc gactatcact tgtttttaat tcataatttg gaataaaagc
    11341 aataaaggaa ctatctggaa acatcacttc caagctttta atatcatttt tcactattga
    11401 tgaaataact aaatcaccaa aaatggccgg tgctacatta tcaggatgtc cttctaatcg
    11461 ggtagctatc tctaattttt ctttttgaga aagagccaaa tttcctaatt gattggctaa
    11521 ttctattcct gcaacaatta cagaggaaga agagcctaaa cctctggcca aaggaatatc
    11581 tgaaaacatt tttaagcggt gtggtgccaa atccgactta acatgcaaag ctgtttgaat
    11641 aaggaggtta gtatgatctt ttggaatatt tactaaattg tgttcaacta accactctgt
    11701 actttcttct aatacttcaa tgattaaata ctttgataac gcaactccta ttgaatcaaa
    11761 gcccggacca atattcgcac tagttgcagg tacaattatc ctcatttcac cctcctatta
    11821 ctttgaaaac attgattaac tgaatgacac cactctcagg aaactcctca ataaattgag
    11881 ttattttctc ttggctaact tcatctgtta atacatagat ctcttgttgt ttatcaccca
    11941 atgctttttc gataacattt ttcaaaccta tattttcttg ctcaaagtac cttccaagtg
    12001 tatcactatc ttcagcattt ctcaaagaaa tagctagata gtactcattg aagatatcag
    12061 actgcattga caacctacac ggacaatgat aagtattcat aggcttaatt tttatctgtg
    12121 atcgaatact acgactaata tcgatgatat ccgctaaaac agaggttgct gttggatttt
    12181 gtcccgcacc tggtccataa aaaagagact gaccaatccc tattgattca atgaatactg
    12241 cattcatcac atgattaact gcagctaagg gatgcttttg agaaattagt gtcggagaaa
    12301 cgtctacact tataccagtt ggagtttcac gaagatcagc taccaactta atggcaaagc
    12361 ccatctcctg agctacttca acatcctctg agcgaatact agaaatcccc ttataattaa
    12421 catcatcaaa atcaataggc ataccaaatc caaattggct taagatagtg gctttgtagg
    12481 cagtatcaat accttcaaca tcatttgtgg gatcactttc agcataacct aactcttgtg
    12541 ccttttttag agcttgttca taagaccatc cctcttcaaa catttttgtt aacatgaagt
    12601 tggaggtacc gttgagaatt cctaataaac gtgtcatttt atctgaggca aacgaatgcg
    12661 atagggtcct taaaatagga atgcctccag caactgctcc ctcatagaat aaagctagac
    12721 ctctagcgtc tgctaaatcc atcaactctt ttccgtgtaa agcaatgaga tctttattgg
    12781 ctgtcacaat atgtttacca aatcctaatg cttgactcaa ataagtttta gctggctcaa
    12841 tcccccccat taattccact acaatatcaa tttgtggatc ttggagaata gtatttatct
    12901 ctgtcacaaa gttataagta aatccctgat tgatgaagcg ctggcggctt tcattatctc
    12961 ttactaatac tttatcaatc acaatatctt cgccttctaa agaaagtagc ttatgttgat
    13021 tttcttttag caaatatgga atacccttag caaccgttcc aaaacctaat aaagctattt
    13081 taatagtcat gaatttctcc ttagaaattg tctctaactt caagacaaac attggctata
    13141 ttataacaaa ttaccaatat tttttatagg gcaaacgcgt cttgtcctat gaaaattctt
    13201 aaaaagagaa tattatttca gaaaagatga tacaataaaa gtactttaaa agttaaggag
    13261 aaataaatgg cacacacacc tactagtcat cgtaaaccac gaaaacgttc cccatggtta
    13321 gctattgctt ctgtcttttt tttgttaata gctttaatag gcatatttct attcttcaac
    13381 aatcgttcaa agcaagaaat aaaaacaaaa acgaacgcct ctagtcatcg aaaaattgtc
    13441 acatctatca aaaaaaagaa atggattaag caaaagactc ctgtcaaaat acccatttta
    13501 atgtaccatg ctgttcatgt tatggaccct tctgaagccg ccagtgctaa cttaattgtg
    13561 gcacctgaca tttttgaaag tcatataaaa cgtctcaaaa aggaaggata ttacttctta
    13621 gcccctaacg aggcatatcg tgcactaaac gaaaatgcct tacctgacaa aaaagtcatt
    13681 tggattactt ttgatgatgg aaatgctgat ttctacacta aggcataccc tatactcaaa
    13741 aagtacaagg ttaaagccac taataatatc atcactggct ttgttcaaga gggacgagaa
    13801 tctaacctca acgttcaaca aatgctagaa atgaagcaaa acggcatgtc attccaagga
    13861 catacggtta ctcaccccaa tctttctctc ttaactccag aactacaaac ccaagaaatg
    13921 acactatcaa aacagttttt agatcaaaaa ctctctcaag atactcttgc gattgcttat
    13981 ccatcagggc gctacaatcc tacaaccttg gatattgcta gtcaatatta taaactaggc
    14041 cttacaacaa acgaaggagt tgcaacaaaa gataacggtt tgctttctct taatcgagtt
    14101 cgtatcttac caactactag cgatgatgat ttgatcaaga cgattaacca ataaaaagaa
    14161 gccttttggc ttctttttat tcttcataag atttaaatgg ttcattttta cgtggataac
    14221 gatctggaga aattgataag cccatttctt ttttctcatg gaaaagttcc actaatagcg
    14281 ctagcatcat caaaatcatt acgaatgaaa atggcagagc gacaataatt aaaacgttct
    14341 gcagcgctaa cagaccacca gacaagagta agacaatagc aatcactgcc aacactagcc
    14401 cccaaattac tttagttcga ttttttggat ttaaattacc atcttctgtc aacatagcca
    14461 aaacgtacgt tgcagaatct gctgatgtaa taaaaaatga aaaaattaaa atgatagcaa
    14521 taatggataa aagccaacct aaagtgtagt gattaaaagt agcaaacaat acttcctctg
    14581 ttgcaaactt tgtcaaatta gtacccagtt gttgtacctg agttgataat gtgccaaatg
    14641 ctgcaaacca tacaaaactc aataatgtcg gtattaaaag aactaccgtt aaaaactcac
    14701 gaatactacg ccctttagaa atacgagcga tgaaaactcc aacaaaagga gaccaggaaa
    14761 tccaccacgc ccaataaaaa atcgtccaat tatcaatcca agaacgttta gtattgtcaa
    14821 aagcagctgc acgaaagctc attccaaaga aattttgtaa ataagagcct aaagactctg
    14881 ttagggtatc aaaaatacga accgttggtc ccaatacaat aactaaagct aagagggcta
    14941 ccgctaaaat caaatttaag ttcgataaaa ttttaacacc ttttcctaaa cctgataaag
    15001 ctgacataac aaataaagct gttgtaataa ggataataac aatttgaaca aatgcattat
    15061 tgggaacacc caataaaaaa cttaaccctc cgttgatttg agcagctcca aatccaagtg
    15121 tcgtagcaac tccaataact gtagcaacaa ctgtggtgat atcaacaatt tttcctagcc
    15181 aaccatctgt cttatcaccg aaaagaggct ttaaagtgac agacaagagg tatttctctt
    15241 gctttcgaaa tccaaaataa gctagagcca aagcaaccaa tgcatatact gcccaagcat
    15301 gtatccccca gtgaaaaaat gtaaaacgaa atgcatcagc taatgctgtt tgcgattcct
    15361 taggtgcacc aggtgtcgaa atagcaaaat gcgataaggg ttcagctgct ccgtagaaga
    15421 ccaaaccaat ccccatacct gctgaaaaca tcattgctat ccatgataca gttgaatatt
    15481 caggacgtga atgaggttgc cctaaacgta tttgtccaag aggactcata ataaaaaaga
    15541 cacacaaagc aagaattgaa gtaactaaca acaagtacaa ccaaccgaaa ttggtagata
    15601 cctgttcacg caagatttgt gtccaagatt gaaaaccacg aggaacaata attcctaata
    15661 agactaaaat aagtgataca attagtgaac ctgtaaatac aggcgtaata tgttttttac
    15721 tcaaaatttc ctcctatact aaaatagtat agcatacttt aataaatact cctctcattt
    15781 taaccacctg tatcaagacc tctccttgtc tcctcaacaa aaaagaacca aataacatta
    15841 tctggttctt gtgtttgact tagtgattaa tcactatttg gtgtaaatac caaatgttcg
    15901 ttgacaaagg catcaaatcc aagttggctc aactcacgac catatcctga atttttaata
    15961 cctccaaacg gtaattccgg taatgatgtc caccctgaat taataaatgt catccctgtt
    16021 tcaatctgag ccgccacttt tttagcatgt tctggatcag aagaaaaaac agtgcttcct
    16081 aaaccataac tagaatcatt agctaaagca atagcttctt cttcagtatc cactttatag
    16141 atagaggcta cggggccgaa aatttcttga ttatagattg gatttgcttt agtgatattc
    16201 gttaaaacag ttggcatgac gaagtttcct ggatgatcta tagtgtcatt tccaaaaact
    16261 acctcagcac cgtggtctac cgccaattta atttgtttta aaacatcatc ctttgcacct
    16321 gcagatgaca gtggagccaa ggttgtttcc gaatccattg ggtcacccca tttagcagac
    16381 ttaaaggtct caactaccat atttacaaat tcgtcatact tatcagccat gacaatgaaa
    16441 cgttttgaag atgtacatac ttgacctgca ttatataaac gcgcaaagaa tattgtttta
    16501 ctaagtaaat caaagtcagc atcatctaaa ataagaaaag catcgttccc acctaattcc
    16561 atagacgatt ttttcaaatt tttaccagcc tcagctgcta ttgatgcccc accacgctca
    16621 gaaccagtta aacacacacc tgcaacacga ggatctgata taaggtttga aacttgatca
    16681 tacgaagcaa aaatattttt aaacgctcct tctggagctc ctgcttctcg gactaaatcc
    16741 tcaaacgctt gagctgaggc aggacaaata gaagcatgtt ttaaaagcat agtattaccc
    16801 actataaagt taggggcaaa tacacgcata atctgataaa atgggaaatt ccatggctca
    16861 acagcaagta aaacaccgac agcttgcttc aaatagtaag cctctccatt tggactttct
    16921 aacggaacag gttttaaaaa cttttgccca ttatcagcat aatagtcagc tatgtctgca
    16981 cacaagtcca cttctccttg tgcttcagtg aaaagttttc ccatatcttt agtcataact
    17041 tcagcgtact tatcacgatc cttacgtaat aaatctgcta ctttatgcaa ttgattttgg
    17101 cgttcttcaa cattatcttc tttgcgccat gtcttgtaga gggcatgcgc tatgtctaat
    17161 gactgttcga gatcactgtc actgatatta tcgaattcat gcaatacttc gttagtgtac
    17221 ggataaattg ttttatatgc cataatgttc cctccttttg atatcctcat tataacattt
    17281 atgaaatcga ttacaaatat ttagctttag acaagatcac caagttccaa ggatagcttg
    17341 cattgttaaa ctaactataa taatgcctaa ccaacacgct gcacctaaaa gaattgattt
    17401 tcctccagtt ttaattagtt tgctgacatt agtatttata ccaattgccc ccattgccat
    17461 aacaataaaa aatttagata aaaccttaag tggctcaaag atacgtaacg aaaaacctaa
    17521 tgaagcaact atcgttgtta gcaaagaagc taagataaaa taaagcacaa acttcggaaa
    17581 tatcttagta acacttacag attgttcttt ctgcgtttgt ttcatatgat aaatagataa
    17641 tacaatcgta attggaatga tggctaatgt tcgtgtcaat ttaacaatag tagctccgcc
    17701 caaagtattg ctgtgattga tagcatccca tgcagtagca gttgctgtaa ctgacgaggt
    17761 atcattgaca gcagtccctg caaataatgc aaaaccgtga tcagagagac caataaaatt
    17821 ccccagcgtt ggaaaaatta aagccgctaa gatattgaat aaaaaaataa ccgaaatagc
    17881 ttgtgctact tcatcatctt tagcattaat aactggtgca gtcgctgcaa ttgcggatcc
    17941 accacaaata gatgaaccaa cacctattaa agtagcaatc gttttatcta acttaaataa
    18001 cttttgcaaa acataagcaa taatcaatga aatggaaatc gtcataataa tgatgggtaa
    18061 cgatgatata ccaactttca tcacttgcat gaggtttagt ccaaaaccca ataagacaac
    18121 tgctgtttgt aaaatatatt tagacgtaaa agcaataccc ttattaaaca attgtcgatt
    18181 ttgataaaag gatccaacaa ttattccaat caagatagca aagactggtg ctcctattaa
    18241 agggagataa agcccaagca accaagatgg tatagcaata ataaagcata atattagtcc
    18301 aggaattttt tccttaaata acatgatagt cctcctcaca ttgactatta tatcacaagc
    18361 tttaaaacgt ggcaggaaaa ccgaaagagg accagaatat gctctgatcc tctttcatta
    18421 ctaattcaca attatttagt tactttcata aatggatatg atttttcatt acctaaaatc
    18481 aatttagagc cctctttttt ataggctgtt ttttcaccat ttttcacaat attaccatag
    18541 aacgcatttg cctttttatt gatatgcgat actttaataa cacttgttaa gcgatgtact
    18601 ttaccatcca taactggcaa tgtcatttca ccagtattag tattatattt tcctccggca
    18661 gcttgtgcag cactttggaa acttctatca atttgttggt caatttgttg gtcagtgatt
    18721 tttgtttcat caaaaatctt tttacctgct tcacttaaat cattgtaagt caaaccttga
    18781 tcttttaatt gtttttcaag agctttatca ataaaagttt ttatagcttg atggaatttt
    18841 acctcatcaa tttgagcagt gactgttatc tttgcctcat cgtttttgac gttcatatgc
    18901 atattagcac ccttaaccaa atcagctgca ctaatcccca tatccgctgc ttgacttcca
    18961 agttgttgtt ctatttcttt ctcaacttgc ttctctaaag aaactgtacg ccatttcccc
    19021 tctagtgact gatgattatt ccagaaatag agaccgcctg cacctacaat taaaataagt
    19081 actagtacag ttaaaaaaac ttttaaaaa
//
LOCUS       DATRNK010000042        18669 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.042, whole genome shotgun sequence.
ACCESSION   DATRNK010000042 DATRNK010000000
VERSION     DATRNK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18669
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.042"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            131..1003
                     /locus_tag="VBC52_001383"
     CDS             131..1003
                     /locus_tag="VBC52_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6882968.1"
                     /translation="MSKKIKNFILLNIGSLIVAFGFNAFFLHNHIVTGGISGLAVSFH
                     EVFGWDSAFFIMIINIPLLLLCYFGLGKQTFLKTVYGSWIFPVFIKLTQSVPTLTHNP
                     LLAALFGGVIVGCGLGIVFWSDSSTGGTGIIIQFLGKYTPISLGQGVILIDGLVTIVG
                     FLAFDSDTVMFSIIGLITISYIINAIQTGFTTLSTVLIVSQEHQKIKTYINTVADRGV
                     TEIPVKGGYSGTNQIMLMTTIAGYEFAKLQEAIAEIDETAFITVTPTSQASGRGFSLQ
                     KNHGRLDEDILMPM"
     gene            1069..2688
                     /locus_tag="VBC52_001384"
     CDS             1069..2688
                     /locus_tag="VBC52_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6882969.1"
                     /translation="MLTVSDVSLRFSDRKLFDEVNINFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHIALGPDERLSVLRQNHFDYEDERVIDVVIMGNETLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNISEELHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKESSELAARLQADRNAKAEEKIKQLQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFVNFKAEREMGNDLLTVENLSVTIDGEKI
                     LDNISFILRPGDKTALIGQNDIQTTALIRALMGDIEYEGTIKWGVTTSRSYLPKDNSR
                     DFASGESILEWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKDFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVVDRIDETYDEFLENAEVQAKVAQLWK"
     gene            2811..5390
                     /locus_tag="VBC52_001385"
     CDS             2811..5390
                     /locus_tag="VBC52_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEO6882970.1"
                     /translation="MTINHYKLRIPYYTISFLLPFIIIVCILFTKNIYWGSPTTILAS
                     DGFHQYVIFNQALRNILHGSNSLFYTFTSGLGLNFYALSSYYLGSFLSPIVYFFNLKN
                     MPDAIYLLTICKIGLIGLSMFVTLCKRHCKVNRILLLVISTCYSLMSFSISQIEINMW
                     LDVFILIPLVVLGVDQLLWERKPILYFLSLTALFIQNYYFGFMTAIFTSLYFIVQITR
                     NTDSTVAFKQFLHFTFLSLLAGMTSSIMILPTYFDLTTHGEKLTKVSKMFTENSWYMD
                     LFAKNMIGAYDTTKFGSIPMIYVGLLPLLLSLLYFTIKEVPRRTRLAYGFLLIFVIAS
                     FYITPLDLFWQGMHAPNMFLHRYSWVLSVLICLLAAECLEYLDNISWKKILGVNLILV
                     SGFIITFLFKKHYHYLNLELLLLTLTFLSAYIILTISFVSKQIPKLVFYPFLIGFVVL
                     EMTLNTFYQLNSLNNEWVFPSRQGYAKYNHSISKLIRKTERNSSTFFRTERWLGQTGN
                     DSMKYNYNGISQFSSIRNRSSSQVLDRLGFKSDGTNLNLRYQNNTLIADSLFGVKYNL
                     TEYPFDKFGFIRKAQDKQTILYKNQFASQLAILTNQVYQDKPFTVNTLDNQTTLLNQL
                     SGLKETYFDHLIPNSVSGQTTLNKQVFVKKNKQGNTEITYNITIPKNSQLYVSMPFIN
                     FNNEENKIVQISVNNGPFVPNTLDNAYSFFNIGSFAENSRIKVKFQFPHNDQVSFPIP
                     HFYGLKLEAYQKAMTVINKRKVKVRTDHNKVIANYTSPNRSSLFFTIPYDRGWKAYQN
                     NKEIKIFKAQKGFMKINIPKGKGKVTLIFIPYGFKFGVGLSITGIVLFTVYYFKFGKN
                     KIG"
     gene            complement(5457..5530)
                     /locus_tag="VBC52_001386"
     tRNA            complement(5457..5530)
                     /locus_tag="VBC52_001386"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5494..5496),aa:Asn,seq:gtt)
     gene            complement(5560..5631)
                     /locus_tag="VBC52_001387"
     tRNA            complement(5560..5631)
                     /locus_tag="VBC52_001387"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5596..5598),aa:Glu,seq:ttc)
     gene            5823..5896
                     /locus_tag="VBC52_001388"
     tRNA            5823..5896
                     /locus_tag="VBC52_001388"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5857..5859,aa:Arg,seq:tct)
     gene            complement(5969..6448)
                     /gene="rlmH"
                     /locus_tag="VBC52_001389"
     CDS             complement(5969..6448)
                     /gene="rlmH"
                     /locus_tag="VBC52_001389"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEO6882971.1"
                     /translation="MKLKIITVGKLKEKYLKEGVAEYQKRLNRFSKIETIELADEKTP
                     DKASISENQRILDIEGERILSKIGERDYVIGLAIEGKQLPSESFSHLIDQKMISGYST
                     ITFVIGGSLGLSPKVKKRADYLMSFGLLTLPHQLMKLVLMEQIYRAFMIRQGTPYHK"
     gene            6649..7878
                     /locus_tag="VBC52_001390"
     CDS             6649..7878
                     /locus_tag="VBC52_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEO6882972.1"
                     /translation="MKKKLVSSLLKCSLIIIVSFAGGAFASFVMNHNDNIPNGGVTKT
                     SKVNYNNITPTTKAVKKVQNSVVSVINYKQQESRSDLSDFYSHFFGNQGGNTDKGLQV
                     YGEGSGVIYKKDGKNAYVVTNNHVIDGAKQIEIQLADGSKAVGKLVGSDTYSDLAVVK
                     IPSDKVSNIAEFADSSKLNIGETAIAIGSPLGTEYANSVTQGIVSSLKRTVTMTNEEG
                     QTVSTNAIQTDAAINPGNSGGALINIEGQVIGINSSKISSTSNQTSGQSSGNSVEGMG
                     FAIPSNDVVKIINQLESNGQVERPALGISMAGLSNLPSDVISKLKIPSNVTNGIVVAS
                     IQSGMPAQGKLKKYDVITKVDDKEVASPSDLQSLLYGHQVGDSITVTFYRGENKQTVT
                     IKLTKTSKDLAKQRANN"
     gene            7976..8749
                     /locus_tag="VBC52_001391"
     CDS             7976..8749
                     /locus_tag="VBC52_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEO6882973.1"
                     /translation="MEYLETININHIAPNPYQPRLEFNTKELEELADSIKINGLIQPI
                     IVRPSAVFGYELVAGERRLRAAKLAKLESIPAIIKSYNNDDSMQLAIVENLQRSNLSP
                     IEEAKAYSQLLQKKSMTHEELAKYMGKSRPYISNTIRLLNLPPLITSAIEEGKLSSGH
                     ARALLSLPDASQQKDWYQRILTEDISVRRLEKLLKQEKKTNHKSLQNKDVFLKHQENE
                     LAQFLGSKVKLTINKDGAGNIKIAFANQEELNRIINTLK"
     gene            8958..10319
                     /gene="dnaA"
                     /locus_tag="VBC52_001392"
     CDS             8958..10319
                     /gene="dnaA"
                     /locus_tag="VBC52_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEO6882974.1"
                     /translation="MTENEQLFWNRVLELSRSQIAPAAYEFFVLEARLLKIEHQTAVI
                     TLDNIEMKKLFWEQNLGPVILTAGFEIFNAEITANYVSNDLHLQETSFSNYQQSSNEV
                     NTLPIRKIDSNLKEKYTFANFVQGDENRWAVSASIAVADSPGTTYNPLFIWGGPGLGK
                     THLLNAIGNQVLRDNPNARVLYITAENFINEFVSHIRLDSMEELKEKFRNLDLLLIDD
                     IQSLAKKTLGGTQEEFFNTFNALHTNDKQIVLTSDRNPNQLNDLEERLVTRFSWGLPV
                     NITPPDFETRVAILTNKIQEYPYDFPQDTIEYLAGEFDSNVRELEGALKNISLVADFK
                     HAKTITVDIAAEAIRARKNDGPIVTVIPIEEIQIQVGKFYGVTVKEIKATKRTQDIVL
                     ARQVAMYLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMVAQDDNLRIEIETIKN
                     KIR"
     gene            10474..11610
                     /gene="dnaN"
                     /locus_tag="VBC52_001393"
     CDS             10474..11610
                     /gene="dnaN"
                     /locus_tag="VBC52_001393"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEO6882975.1"
                     /translation="MIHFSINKNFFLHALTVTKRAISHKNAIPILSTVKIEVTRDAII
                     LTGSNGQISIENTIPASNENAGLLVTNPGSILLEAGFFINIISSLPDVTLEFTEIEQH
                     QIVLTSGKSEITLKGKDVDQYPRLQEMTTDTPLTLETKLLKSIINETAFAASQQESRP
                     ILTGVHLVISQNKYFKAVATDSHRMSQRTFQLEKSANNFDLVVPSKSLREFSAVFTDD
                     IETVEVFFSDSQMLFRSENISFYTRLLEGNYPDTDRLLTNQFETEIIFNTNALRHAME
                     RAYLISNATQNGTVRLEIQNETVSAHVNSPEVGKVNEELDTVSLKGDSLNISFNPTYL
                     IESLKAVKSETVTIRFISPVRPFTLTPGEDTEDFIQLITPVRTN"
     gene            11668..12560
                     /locus_tag="VBC52_001394"
                     /pseudo
     CDS             11668..12560
                     /locus_tag="VBC52_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
     gene            12570..12767
                     /locus_tag="VBC52_001395"
     CDS             12570..12767
                     /locus_tag="VBC52_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEO6882976.1"
                     /translation="MYQVGSLVEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            12970..13086
                     /locus_tag="VBC52_001396"
     CDS             12970..13086
                     /locus_tag="VBC52_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882977.1"
                     /translation="MDNLIRLAEIFDVSLDEHVLAKTNEVKVERIYEKNLDL"
     gene            13330..14445
                     /gene="ychF"
                     /locus_tag="VBC52_001397"
     CDS             13330..14445
                     /gene="ychF"
                     /locus_tag="VBC52_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEO6882978.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEEEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ADPDDIDYVNQIRAFAETESAEVVVISARAEEEISELDDEDKLEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LIQYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            14529..15104
                     /locus_tag="VBC52_001398"
     CDS             14529..15104
                     /locus_tag="VBC52_001398"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEO6882979.1"
                     /translation="MVKMIVGLGNPGSKYNDTKHNIGFMAVDRIVKNLDVNFTEDKNF
                     KAEIGSDFINGEKIYFIKPTTFMNNSGIAVKALLTYYNISIKDMIIIYDDLDMEVGKI
                     RFRQKGSAGGHNGIKSIIAHLGTQEFDRIKVGIGRPNGRMTVINHVLGKFDKNDEIMI
                     SNTLDKVDNAVNYYLQTNDFQKTMQKYNGLK"
     gene            15101..18598
                     /gene="mfd"
                     /locus_tag="VBC52_001399"
     CDS             15101..18598
                     /gene="mfd"
                     /locus_tag="VBC52_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEO6882980.1"
                     /translation="MNIIELFSQNKVVRTWHSGLVTNSRQLVMGFSGASKAIAIASAY
                     EKLSKKIMVVTATQNDSDKLSSDISSLIGEDNVYQFFADDVPAAEFIFSSLDKSISRL
                     SALRFLKDPEKNGVLITSISGLRLLLPNPEVFSKSQYKFEIGQECYLDKLSKNLVNLG
                     YQKVSQVFSPGEFSQRGDILDIFEMTQEYPYRLEFFGDEIDGIRQFDIDTQKSLKQLE
                     SVQISPADDIILQDADFERAKKKLEGYLVTASEVQRAYLSEVLSTTENHFKHSDIRRF
                     LSIFYEKEWGILDYIPEGTPLFVDDFQKIVDRNAKLDLEIASLLTEDLQQGKSHSSLN
                     YFSDPYKQLRQYQPATFFSNFHKGLGNLKFDKLHHFTQYGMQEFFNQFPLLVDEINRY
                     KKSGATVLLQVDSQKGLNLLQENLKEYGLDLIISDKNDIVPKESQLIVGHLSNGFYFA
                     DEKIVLITEREIYHRRVKRKIRRSNISNAERLKDYNELSVGDYVVHNVHGVGKFLGIE
                     TIEIQGIHRDYLTIQYQNADRISIPVEQIELLTKYVSADGKEPKINTLNDGRFKKAKQ
                     RVAKQVEDIADDLLKLYAERSQLQGFAFSPDDNMQNDFDNDFAYVETEDQLRSIKEIK
                     QDMEGNRPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHFENFKE
                     RFSNYPVTVDVLSRFRSKKEQTDTLKRLSKGQVDIIIGTHRLLSQDVVFSDLGLIVID
                     EEQRFGVKHKEKLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLETNPGLVREAIIREIDRGGQVFYVYNKVDTIDQKVSELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFVENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEISEKRLDAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIATKQGKSLIRQKGNAELALQIDAYLPAEYISDERQK
                     IEIYKRIRELETRADYETLQDELIDRFGEYPDQVAYLLEIGLLKAYLDLAFTELVERK
                     GNEISILFEKASLKYFLTQDYFEALSKTQLKARISETNGKMEVVFNIKHKKNYEIIEE
                     LLKFAECFIEIKSRKPVEE"
BASE COUNT         6376 a         2928 c         3286 g         6079 t
ORIGIN      
        1 tctcgctgtt agataatagg taataataag ttttgataaa ataaaaatgt tatgactgag
       61 ttgtcaatat tatttattga cagctttctc tttttatcat gttattatta ctaaagttaa
      121 ggagcttatc atgtctaaaa aaattaagaa ttttatacta ttaaatattg gatctctaat
      181 tgttgcattt ggtttcaacg ctttcttcct tcataatcat attgtcacgg gggggattag
      241 tggtttggca gttagttttc atgaagtatt tggttgggat tctgcttttt ttattatgat
      301 tatcaatatt ccattgctcc ttctttgcta ctttggctta ggtaaacaaa cctttttaaa
      361 aactgtctat ggttcttgga tttttcctgt ttttattaag ttaacacaaa gtgtaccaac
      421 tttgacccac aacccactcc tcgcagcact ttttggaggt gttattgtag gatgtggttt
      481 ggggattgtt ttttggagtg actcttcaac tggtggaacg gggattatca ttcaattctt
      541 aggaaaatat actcctataa gccttggaca aggggttata ttgattgatg gacttgttac
      601 aattgttggt ttcctagctt ttgacagtga tacggttatg ttttctatta ttgggttgat
      661 aactattagt tatattatta atgctatcca aactggtttt acaaccttaa gcactgtctt
      721 aatcgtttct caagagcacc aaaaaattaa gacatatatc aatactgtcg cagatagagg
      781 agtaacagaa attcccgtta aagggggata ttctggaact aatcaaatca tgcttatgac
      841 cactattgct ggttatgagt ttgctaaatt acaagaggca atagcagaaa ttgacgaaac
      901 agccttcata acagtaactc caacatcaca agcttctgga cgtggattta gtcttcaaaa
      961 aaatcatgga cgtcttgatg aagacattct tatgccaatg taatcgattg ataattaaag
     1021 ctttttctag tagaatagca gatagaatat acacaagagg taacaaaatt gcttacagtt
     1081 tcagatgttt cactacgttt tagtgatcgc aaattatttg atgaagttaa cattaacttt
     1141 acagcaggaa atacctatgg tcttatcgga gcaaatggcg ctggtaaatc aactttcctt
     1201 aaaattttag ctggtgatat tgaaccaaca acaggacata ttgcacttgg cccagatgaa
     1261 cgcctttctg tacttcgcca aaatcatttt gattatgaag atgagcgcgt cattgatgtc
     1321 gttatcatgg gtaatgaaac actttatagt atcatgaaag aaaaggatgc tatctacatg
     1381 aaagaagatt tttctgatga agatggtgtt cgcgctgcgg aattagaagg agaatttgct
     1441 gaactcggtg gatgggaggc tgaaagtgag gcttctcaat tactccaaaa tctaaatatt
     1501 tcagaagagc ttcattatca aaatatgagt gaactagcta atggagacaa agttaaagtt
     1561 ctccttgcaa aagctctctt tggtaaacca gatgtacttc tccttgacga gcctaccaat
     1621 ggacttgata tccagtctat tacttggtta gaagatttct tgattgactt tgaaaataca
     1681 gttattgtag tttcccacga ccgtcatttc ttgaataaag tatgtacaca tatggctgac
     1741 cttgattttg gaaaaattaa actgttcgtc ggtaactatg atttctggaa agaatcatca
     1801 gaattagcag ctcgtttaca agctgatcgt aatgctaaag cagaagaaaa aattaaacaa
     1861 cttcaagaat tcgttgcacg attttcagcg aatgcttcaa aatcaaaaca agcaacttct
     1921 cgtaaaaaaa tgcttgataa aattgaactt gaagagattg taccatcaag ccgtaaatac
     1981 ccattcgtta actttaaagc cgaacgcgaa atgggaaatg atttattgac cgttgaaaat
     2041 ctctctgtca ctattgatgg ggaaaaaatc cttgataata tcagtttcat tcttcgtcct
     2101 ggtgataaaa ctgcacttat tggacaaaat gatatccaaa ctactgcact tattcgagca
     2161 ttgatggggg atatcgaata tgaaggaaca atcaaatggg gagtaacaac tagtcgttct
     2221 taccttccaa aagacaattc acgagacttt gcttctggcg agtcaatctt agaatggctt
     2281 cgtcaatttg ctagtaaaga agaagacgat aataccttct tacgtggctt cctaggtcgt
     2341 atgcttttct ctggagatga ggttaacaaa tctgttaatg tcttatctgg gggtgaaaaa
     2401 gtacgcgtta tgctttcgaa attaatgcta ttaaaatcta atgttcttgt tcttgatgat
     2461 ccaacaaacc acttagattt ggaatctatt tcaagtctca acgatggact aaaagatttc
     2521 aaagaatcta ttatttttgc aagtcatgac cacgagttta ttcaaacttt agcgaaccat
     2581 attattgttc tttctaaaaa cggagttgtc gatcgtatcg atgaaactta cgatgaattt
     2641 cttgaaaatg ccgaagtaca agctaaggtc gctcaactct ggaaataatc ctttggcata
     2701 tctctaacct aataaacgtg tcaagattga aaatcttaaa actatattaa tcatttaaat
     2761 aaaaaggttg gaacaaatag tgtccagcct ttttatctta ggagatatat atgacaatta
     2821 accactataa gcttcgtata ccttactata caataagctt tctactcccc ttcataatca
     2881 tagtctgcat cctctttact aagaatattt attggggaag tccaacaacc atattagcaa
     2941 gcgatggctt tcatcaatat gttatcttta atcaagcatt aagaaatatt cttcatggtt
     3001 caaacagctt attctatacc tttacaagcg gtttaggttt aaatttttat gctctgagta
     3061 gttactattt gggaagtttt ttatccccaa tagtttattt ctttaatcta aaaaatatgc
     3121 ctgatgctat ctacctttta acaatatgta aaatagggtt gataggtttg tcaatgtttg
     3181 tcactctttg taaacgtcat tgtaaagtta atcgtatctt acttttagta atttctactt
     3241 gctatagctt aatgagtttc tcaattagcc aaattgaaat taatatgtgg ctagatgtat
     3301 ttattctaat tccccttgtt gttttaggag ttgatcagct actatgggaa agaaaaccta
     3361 tactttattt cctctctctg acagccttat tcatccaaaa ctattatttt ggttttatga
     3421 ctgccatctt tactagcctt tacttcatcg tacaaataac ccgtaatact gatagtacgg
     3481 ttgcatttaa acaatttctt cattttacct ttttatctct tttagctggt atgactagta
     3541 gcattatgat tttgccaact tattttgatt taactactca tggtgaaaag ttgacaaagg
     3601 tatcgaagat gtttacagaa aattcttggt acatggattt gtttgctaaa aatatgattg
     3661 gagcctatga tacaacaaaa tttggctcta ttcctatgat ttatgtaggt ttacttcctt
     3721 tactcctttc tttactttac tttacaataa aggaagttcc tcgaaggact agattagcat
     3781 atggattttt actaatattt gttattgcta gtttttatat aacacctcta gatttatttt
     3841 ggcaagggat gcatgctcca aatatgtttc tccatcgcta ctcttgggta ctttcagtac
     3901 ttatctgtct gctagctgct gaatgccttg agtatttaga caacatttct tggaaaaaga
     3961 ttttaggggt caatttaatt ttagtaagtg gtttcataat cacgttttta tttaaaaagc
     4021 attatcatta ccttaatctt gaattacttc ttttaacctt aactttcctc tcagcttata
     4081 ttattctaac tattagtttt gtcagtaagc aaatacctaa actagttttt tatccctttc
     4141 ttattggttt tgttgtgtta gagatgactt taaacacttt ttaccaacta aatagtctta
     4201 acaatgagtg ggtatttcca tctaggcaag gatatgctaa atataatcac agcattagca
     4261 aacttatcag aaagactgag agaaatagct ctacattctt ccgtacagag cgctggttag
     4321 gacaaaccgg caacgactct atgaaatata attataatgg tatttcacaa ttttcatcta
     4381 ttagaaatcg ttcttccagt caagtgcttg atagacttgg ctttaaatca gatggtacaa
     4441 atttaaattt acgttatcaa aataatactt tgatcgcaga tagtctattt ggtgtaaaat
     4501 ataaccttac agaatatcct tttgataaat ttggttttat cagaaaagct caagataaac
     4561 aaaccattct ttacaaaaat cagtttgcca gtcaattagc tatactaacg aatcaagtat
     4621 atcaagataa accatttact gtgaatactt tggataatca gacaactctt ttaaatcaat
     4681 taagtggttt gaaagagacg tattttgatc atcttattcc aaatagcgtt tctgggcaaa
     4741 ccactcttaa taaacaagta tttgttaaaa agaataaaca agggaatact gaaattacct
     4801 ataacataac tatccctaaa aacagtcagt tatatgttag tatgccgttt ataaatttta
     4861 ataatgagga aaataaaatt gttcaaatct ccgttaataa tggaccattt gtacctaata
     4921 ctcttgataa tgcttattca ttttttaata ttggttcctt tgctgaaaac agtcgtatca
     4981 aagtaaaatt tcaatttcca cacaatgatc aagtttcttt cccaattcct cacttttatg
     5041 ggctcaagtt agaagcttat caaaaagcta tgactgttat taataaacga aaagtaaaag
     5101 taagaactga tcataataaa gttattgcta attatacaag tcctaataga agttctcttt
     5161 tcttcactat tccctatgat aggggttgga aagcttatca aaataataaa gaaataaaaa
     5221 ttttcaaagc tcagaaaggt tttatgaaaa taaatattcc taaaggaaaa ggcaaagtaa
     5281 cattaatttt tatcccttac ggctttaaat ttggggtagg cctatctatt actggaatag
     5341 tattatttac tgtctattat tttaaatttg gaaaaaataa aataggatga gagtattctc
     5401 atcctatttt attaacaaaa aacgccgaca aatcaacgtt tagatgtgtc ttatcactcc
     5461 ggcagcagga ctcgaacctg cgacatcatg attaacagtc atgcgctact accaactgag
     5521 ctatgccgga taaataaaaa actgcaaagc agttatcaat agtccgtacg ggattcgaac
     5581 ccgtgttacc gccgtgaaaa ggcggtgtct taaccccttg accaacggac cataggttag
     5641 gtgtttcctt agtactctta atattatatc agaaaaaaaa ggtttgtcta tacttttgtt
     5701 caataatttt tacaaaaaaa ttgaacctac ttatgagcaa aaaaatatat ttccaactca
     5761 taaatagtac atatgaaaac ttgacagcct gcctaatatt ttggtagaat agcagagttg
     5821 atggttccat agctcagctg gatagagcat tcgccttcta agcgaacggt cgcaggttcg
     5881 aatcctgctg gaatcagata atgaaagcct agatagatta tctaggcttt tttatttgtt
     5941 tttagcagtt gtttcataaa tggaacgatt atttgtggta aggagtgcct tgacgaatca
     6001 taaaagcacg ataaatttgt tccatcaaga ctaatttcat aagttgatga ggtaatgtta
     6061 gcagtccaaa actcattaaa taatcagcac gttttttcac ttttggagat aagcccaaac
     6121 taccaccaat aacaaaagta atagtagagt agccggatat cattttttga tcgattagat
     6181 ggctaaatga ttcagatggc aattgtttcc cttctatagc caaaccaatg acataatccc
     6241 tctcacctat tttactcaaa atacgttccc cttctatatc taaaattctt tgattttcag
     6301 atatactagc tttatcaggc gtcttttcat cagctagctc aattgtctca attttagaaa
     6361 agcgattcag acgtttctga tactctgcaa caccttcttt aaggtatttc tctttcaatt
     6421 ttccaactgt aattattttt aatttcatag ctacattata acataatcca caatattttc
     6481 acaatttatc cacatgttta tccttacata tccccttatt ttataggcat tctataatta
     6541 taatcaaggt tatccacaag ttgtggatat ttcctttttt tagcttttaa ggtataatta
     6601 agaagcttgt cataagatta gtataaaagt ttacggaggt agctatttgt gaaaaaaaaa
     6661 ttagtctcat cacttctaaa gtgttctcta atcattattg ttagctttgc tggtggagca
     6721 tttgctagtt ttgtcatgaa tcataatgac aatattccaa atggtggtgt cactaaaact
     6781 agtaaagtaa attataataa cataacgcct acaacaaaag ctgttaaaaa ggtacaaaat
     6841 agtgttgttt ctgttatcaa ttataaacaa caagagagtc gttctgacct atcagacttc
     6901 tatagtcatt tttttggtaa tcagggaggc aacactgata agggcttaca agtttacggt
     6961 gaaggctctg gagtcatcta taaaaaagat ggtaaaaatg cctatgttgt cactaataac
     7021 cacgtcattg atggggctaa acaaattgaa attcaactag ccgatggctc aaaagcagtt
     7081 gggaaacttg ttgggtcaga tacctactct gatttagccg tcgtcaaaat tccatcagat
     7141 aaagtttcaa atattgcaga atttgctgat tcatcaaaac tcaacattgg tgaaactgct
     7201 atagcgatcg gaagccctct tggaactgag tatgcaaatt ctgtaactca aggtattgta
     7261 tctagtttaa aaagaactgt aacaatgact aatgaagaag gacaaacagt ttctacaaat
     7321 gctatccaga ctgatgctgc tatcaatcct ggtaattcag gtggagcact tatcaatatt
     7381 gaaggacagg ttattggaat taattctagt aaaatttctt ctacatcaaa tcaaacctca
     7441 ggacaatcgt caggaaatag cgttgaaggt atgggatttg ccattccttc aaatgatgtt
     7501 gttaagatta tcaatcaact tgagagtaac ggacaagtag agagacctgc tctaggtatt
     7561 tctatggctg gattaagtaa tttaccatcc gatgttatta gtaaactgaa aatcccaagt
     7621 aatgttacta atggtattgt agtagcatct atccaatctg gcatgccagc tcaaggcaaa
     7681 ctaaagaaat acgatgtcat tactaaagtt gacgataaag aagtagcatc tccaagtgat
     7741 ttacaaagtt tactctatgg ccaccaggta ggagattcca taacagtaac cttttatcgt
     7801 ggtgaaaata aacaaacagt cactataaaa cttactaaaa ctagtaaaga tttagctaaa
     7861 caacgagcaa ataactaaat caattctagt tattttaact aagatatagt aaaattaaag
     7921 gggttggtca gatagaccaa tccctttagt aagtctatag aatctgaggt ctaaaatgga
     7981 atatttagaa acgatcaaca tcaatcatat tgctccaaat ccgtatcaac ctcgcctaga
     8041 atttaataca aaagaactag aagaattagc cgactctatt aaaattaatg gccttattca
     8101 acccattatt gtaagaccgt cagcagtctt tggttacgaa ctggtagccg gagaacgacg
     8161 gctaagagcc gcaaagttag ctaaattgga aagtatacct gcaattataa aatcctataa
     8221 taatgatgat agtatgcaat tagctattgt cgaaaattta caacgatcta atttgtcacc
     8281 cattgaagag gctaaagcct actctcaact tctacaaaag aaaagtatga cacatgaaga
     8341 gttagcaaaa tatatgggaa aatctcgtcc ctatatatca aatacgatac gactactaaa
     8401 tttaccgcca ctaattactt cagctattga agaaggaaaa ctttcctctg gtcatgcgcg
     8461 tgcactactc agtctaccag atgctagtca acagaaagat tggtatcaga gaatcctcac
     8521 tgaagatatt agtgtaagac gactagagaa gttattaaaa caagaaaaga aaacaaatca
     8581 taagtcactt caaaataaag atgtctttct aaaacatcaa gaaaatgagt tagctcaatt
     8641 tcttggtagc aaagtgaaac ttactattaa taaagatggt gctggcaata taaagattgc
     8701 tttcgctaac caagaagaat taaacagaat tatcaacact ctaaaataag ggtgttgatt
     8761 atttttttat ttaatcaaac ttatccacaa ggtattttgc tatttttctg ttgattctct
     8821 aagcttttct aatttccaca gactgtggaa aactttaatt aacattgttg attttattct
     8881 tcaacatctg tggaaaactt tattttttta tggtacaata taacaataat tatccacaag
     8941 acaataagga agaagctatg acggaaaacg aacaactatt ttggaataga gtactagagc
     9001 tatctcgttc tcaaatagca ccagcagctt atgaattttt tgttctagag gctagactcc
     9061 tcaaaattga acatcaaact gcagttatta ctttagataa cattgaaatg aaaaagctat
     9121 tctgggaaca aaatttgggg cctgttatcc taacagctgg ttttgaaatt ttcaatgctg
     9181 aaattacagc taactatgtc tcaaacgatt tacatttaca agaaactagt ttttctaact
     9241 accagcaatc tagcaatgaa gtaaatactt taccaattag aaaaatcgac tctaatctta
     9301 aagagaaata tacttttgct aattttgttc aaggagatga aaatagatgg gctgtttcag
     9361 catcaattgc tgtagctgat agtcctggca cgacttataa tcctctattt atctgggggg
     9421 gacctggtct aggaaagacg catctactaa atgctattgg aaatcaagtc ttaagagata
     9481 atccaaacgc gagggtttta tacatcactg ctgagaattt tattaatgaa ttcgtcagtc
     9541 atattcgttt agattcgatg gaagaattaa aagaaaagtt tcgcaacttg gacttactcc
     9601 tgattgatga tattcagtcg cttgctaaga aaaccttagg ggggacccaa gaggagttct
     9661 tcaatacttt caatgcttta catacaaacg ataaacaaat cgtattgacc agtgaccgaa
     9721 atccaaatca attaaatgat ctagaagaac gtctagtcac gcgctttagt tggggactcc
     9781 cagtaaatat cacaccacct gattttgaaa cacgagttgc tattttaacc aataaaattc
     9841 aagaatatcc ttatgatttt cctcaagata ccattgaata cttagcagga gaatttgatt
     9901 ccaacgtacg tgaattagaa ggagccttga aaaatattag tctagttgct gactttaagc
     9961 atgctaaaac tattacagta gatatagctg cagaagctat cagagcacgt aaaaatgacg
    10021 gtcctattgt tactgtcatt cctatagaag aaattcaaat acaggttggt aaattctatg
    10081 gcgtaactgt aaaagagata aaagcaacta aaagaacaca agatattgtc cttgcaagac
    10141 aggtagccat gtacttagct cgtgagatga cagataacag tctccccaaa ataggtaaag
    10201 aatttggggg acgagatcac tcaactgttc tccacgctta taataaaata aaaaatatgg
    10261 ttgctcaaga tgacaactta cgaattgaga tagaaactat caaaaataaa atcaggtagc
    10321 ctttgtggat aagtatcaga aaaatacgag acttttccac aggttgtgaa caagttattt
    10381 tccttatgaa tactaaagtt ataatactta ttcacaactt taacaggacc tactattact
    10441 actaaattta tactaatata taaaggatat actatgattc atttttcaat aaataaaaat
    10501 ttcttcttgc atgctctaac ggtaaccaaa cgagctatta gtcataaaaa tgcgattcca
    10561 atcctttcaa ctgttaaaat agaagtgact agagatgcta tcattttaac ggggtcaaat
    10621 ggacaaattt caattgaaaa tactattcct gcttcaaatg aaaacgcagg tttactagta
    10681 acgaatccag gctctatttt gttagaagct ggtttcttta ttaatattat ttcaagttta
    10741 ccagatgtaa ctttagaatt tacagagatt gaacaacatc aaattgttct tactagtgga
    10801 aaatcagaga ttactttgaa aggtaaggat gtcgatcaat accctcgtct gcaggaaatg
    10861 acaacagata ctccattaac attagaaact aaactgttaa aatcaattat taatgaaact
    10921 gcttttgctg ctagccaaca agaaagccgt ccaatcttaa caggtgttca tttggttatc
    10981 agtcaaaata aatactttaa ggctgttgcg acagattcac accgtatgag tcaacgcact
    11041 ttccaattag agaaatcggc taataatttt gatttggttg ttccaagtaa atcccttcga
    11101 gaattttcgg ctgtttttac agatgatatt gaaactgtag aggttttctt ctcagatagt
    11161 caaatgttat ttagaagtga aaatatcagc ttctatacac gtttgcttga aggaaactac
    11221 cctgatactg atcgcctcct aactaatcag tttgaaaccg aaattatctt taatacaaat
    11281 gctttacgcc atgctatgga acgtgcttat ttaatttcga atgcaactca gaacggtact
    11341 gttcgtttag aaattcaaaa tgaaacagtc tcagctcatg taaactctcc agaagttggt
    11401 aaagttaatg aggaattgga tactgttagc cttaaaggtg atagtttaaa tattagtttt
    11461 aatccaactt acctaattga atctttaaaa gcagtaaaaa gcgaaacagt tacgattcga
    11521 tttatttctc cagtacgtcc atttactttg acacctggtg aagatactga agatttcata
    11581 caattaataa ctcctgttcg tactaactaa ataagaaaac tacccaagtt attcttgggt
    11641 agttttctta ttctaagata tgctataatg atgagtaata tgactctata tataatagct
    11701 aacccccatg ctggtaataa aaatgcctcc actattgttg gtcaaattca ggagttttat
    11761 catactgaag atatttctgt gttctataca gaacagaaag atgatgaaaa aaaacaagtc
    11821 attaatatac taaggtcttt taaagaaagt gatcatctaa tgattatagg aggagatggt
    11881 accttatcaa aagtaatgac ttatctcccc aacatattcc gtgcgcttat tatcctgttg
    11941 gttcgggaaa tgattttgcc agagctttga aaattcctaa cctcaaagaa acccttaccg
    12001 ctattcagac agaaagatta aaggaaatta attgctttat ttatgataaa gggctgattt
    12061 tgaatagttt agatttaggt tttgcagcct atgtcgtttg gaaagcgtct aactctaaaa
    12121 taaaaaatat tttgaataga tatcgtctag gaaaaataac ttacattgtt atagctatca
    12181 aatcattatt acattcttct aaagtacaag tattagtgga aggagagaca ggacagcgga
    12241 taaaactaaa tgacctttat tttttcgcat tagcaaataa cacttatttt ggaggtggca
    12301 ttactatttg gcctaaagca agtgctttaa ctgcagagtt agatatggtt tacgctaaag
    12361 ggcatacttt tctaaaacga ttaagtattt tattatcttt ggtttttaaa aggcatacta
    12421 cctctaaaag tataaaacac cagactttca aagctatgac cgtatatttt cctaaaaata
    12481 gtttgattga aatagatggc gaaatagttg agttagatca gattagttta aaatgtcaaa
    12541 aaaggtattt atatatgtaa ggagaaaaga tgtatcaagt tggatcactt gttgaaatga
    12601 aaaaaccgca cgcctgtgtg attaaagaga ctggtaaaaa agctaatcaa tggaaagtgc
    12661 ttagagtagg agctgatatt aaaattcaat gcactaactg tcagcatgtg attatgatga
    12721 gtcgttatga ttttgagcga aaattaaaaa aagtactgca accatgaaag tccttttaaa
    12781 caaataatta tagctatatt tgcaaccaat actttcctaa ataattgcta gtatgccgtt
    12841 ggaaaattag ctattctaat gttatcgaaa gaagaaaggt ggccattgac aatgacgaaa
    12901 gttgcagaac agttgtttat ttttcggtaa gtcatatcaa aatgggaaaa tggggatacc
    12961 acaccagatt tggataattt aatcaggtta gctgagattt ttgacgtgag cttagatgaa
    13021 catgttttag ctaaaacaaa tgaagttaaa gttgaacgta tttatgaaaa aaatcttgat
    13081 ctgtaaaaat ataataaaat ttattggtta atttttcgaa atattattct atttctacta
    13141 attatcttag ctatattaac tttcttataa gctctaggtc ttccctttgt ttcggagtgg
    13201 ataatttaaa ttagggtgaa agagtagcat aatgataagc aaatctgaaa ttttcttctt
    13261 atcataccgc tttttcttct attttttgat ataatagtct tgattgaaat ttgaatggag
    13321 actcactaaa tggctttaac agcaggtatt gtaggcttac caaatgttgg taaatcaact
    13381 ttatttaacg caattacaaa agcaggagca gaagctgcta attatccttt tgcgactatt
    13441 gatcccaatg ttggtatggt agaagtacca gatgagcgtc tacaaaaatt gacagaattg
    13501 attacgccta aaaaaacagt tccgacaacc tttgaattta ctgatattgc aggtattgtt
    13561 aaaggtgctt cgaaggggga aggtctaggt aataaatttt tagctaatat tcgtgaagtg
    13621 gatgctattg ttcatgtggt acgtgctttt gatgatgaaa atgttatgcg tgaacaaggt
    13681 cgtgaggatg ccttcgttga tcctatagca gatattgata ctattaatct tgaattaatc
    13741 ttagctgatt tagagtcaat caataaacgt tatgcgcgtg ttgaaaaaat ggcacgaact
    13801 caaaaagata aagaatcagt agcagagttc aatgttttac aaaagattaa acctgttttg
    13861 gaagatggga aatcagctcg taccattgag tttacagaag aggaagcaaa agttgttaaa
    13921 ggtctctttt tattaacaac taaacctgtt ttatatgttg ctaatgttga tgaggacaaa
    13981 gtagctgatc ctgatgatat cgactatgtt aatcaaattc gtgcatttgc agaaacagag
    14041 agtgcagaag tagttgtgat ttctgctcga gctgaagaag aaatatctga acttgatgat
    14101 gaagataagt tagaattttt agaagctatt ggattaacag aatctggtgt tgataaatta
    14161 acacgagctg cttatcatct tttaggcctt ggaacttact ttactgctgg tgaaaaagag
    14221 gttcgcgctt ggacatttaa gcgtggtata aaagctccac aagctgcggg tattatccat
    14281 tcagattttg aaagaggttt tattcgtgca gtaacgatgt catatgatga ccttattcaa
    14341 tatggatctg aaaaagcagt aaaagaagct ggccggttac gtgaagaagg aaaagagtat
    14401 attgttcaag atggcgatat catggaattc cgtttcaatg tctagtaata aataatcaat
    14461 ttgttaactt ttaggttaag gatattgtta ttatccttaa ccatttttgc gttttaatgg
    14521 agaagataat ggtaaaaatg attgttggat taggaaatcc aggaagtaaa tataatgata
    14581 caaaacataa tattggattt atggctgttg acagaattgt aaaaaacctt gacgtcaact
    14641 ttacagaaga taaaaatttt aaagcagaga taggtagtga ttttatcaac ggtgaaaaaa
    14701 tttattttat caaaccaact acttttatga ataacagtgg tattgctgta aaagccctac
    14761 ttacttacta caacatttct attaaggata tgattatcat atatgatgat ttagacatgg
    14821 aagtcggtaa aatacgtttt cgtcaaaaag gatctgctgg gggacataat ggtatcaagt
    14881 ctattattgc tcatttaggt actcaagaat ttgatcgtat taaagtaggt ataggacgtc
    14941 cgaatggacg tatgacagtt attaatcatg ttctagggaa gtttgataaa aatgatgaaa
    15001 ttatgatatc aaatactctt gataaggttg acaatgctgt caattattat ttacagacga
    15061 atgattttca aaaaacaatg cagaaataca acggtttaaa atgaatatta tagaattatt
    15121 tagccaaaat aaagtggtgc ggacatggca ctctggatta gtaactaata gtcgccaatt
    15181 agttatgggg ttttcaggag caagtaaagc tattgccata gcatcagcct acgagaagtt
    15241 atctaaaaaa attatggtag tcactgccac tcaaaacgat tctgataaat tatcaagtga
    15301 tatatcaagt ttaattggag aagataacgt ttatcaattt tttgctgatg acgttcctgc
    15361 agcagagttt attttctctt cattagataa atcaatttca cgactcagtg ccttgaggtt
    15421 tctaaaagat ccagaaaaaa acggtgtttt gattactagt atcagtggtt taaggttact
    15481 attaccaaat ccggaagttt tctcgaaaag tcaatataag tttgaaatcg gtcaagaatg
    15541 ttatttagat aaattgagta agaacctagt caatttaggt tatcagaaag tttctcaagt
    15601 atttagtcct ggtgaattta gccaaagagg agatatttta gatatttttg agatgacaca
    15661 agaatatcct tatcgcttgg aattcttcgg agatgaaata gatggcatca ggcagtttga
    15721 tattgacact caaaagtctc ttaaacaatt agagtcagta cagatttcgc cagcagatga
    15781 tattatttta caagatgcag atttcgagcg tgcaaagaaa aaacttgagg gctatctagt
    15841 aacagctagt gaggtacaac gcgcctattt gagcgaggtt ttatctacaa ctgaaaatca
    15901 ttttaagcac tcagatattc gtcgttttct aagtatcttt tatgaaaaag aatggggaat
    15961 attagactat attcctgagg gaactccact ttttgttgat gattttcaaa aaattgtaga
    16021 tcgaaatgct aagttagatt tagaaatagc tagccttctg acggaagatt tacaacaagg
    16081 caagtctcat tctagtttga actatttttc agatccttat aagcaattaa gacaatacca
    16141 accagcaacc ttcttttcaa atttccacaa aggattagga aatctgaaat ttgataaact
    16201 tcatcatttt actcaatatg gaatgcaaga attttttaat caatttccat tgttggtcga
    16261 tgaaatcaat cgttataaga aatctggagc gacagtactg ttgcaagtag attctcaaaa
    16321 aggtttaaat ctccttcaag aaaatttaaa agaatatggg ttagatttaa tcatttcaga
    16381 taaaaacgat atcgttccaa aggagtcaca acttattgtt gggcatcttt caaatggttt
    16441 ttattttgca gatgagaaaa ttgttctgat tactgaacgt gagatttatc ataggcgtgt
    16501 taaaaggaaa attagacgca gtaatattag taatgcagaa cgtctaaaag attataatga
    16561 actttcagtt ggcgattatg ttgttcataa tgttcatggc gtaggaaaat ttttaggtat
    16621 tgaaacaatt gagatacaag gtattcatcg tgattatctg actattcaat atcaaaatgc
    16681 tgatcgtatc tctatccccg tagaacaaat tgaacttcta acaaaatatg tctctgcaga
    16741 tggcaaagag cctaaaatta atacgttaaa tgatggtcgc ttcaaaaaag caaaacagag
    16801 agttgcaaag caagtagaag atattgctga tgatttgttg aaactttatg cagagcgtag
    16861 tcaattacaa ggatttgcat tttctcctga cgataatatg caaaatgact ttgataatga
    16921 ttttgcatat gtagagacag aggatcaatt gcggtctata aaagaaatta aacaggatat
    16981 ggaaggtaat cgtccgatgg atcgattatt ggttggagac gttggttttg gtaagacaga
    17041 ggttgctatg cgtgcggctt tcaaagctgt caatgaccat aaacaagttg ctgttttagt
    17101 cccaacaaca gttctagctc agcagcattt tgaaaatttt aaagaacgtt ttagcaatta
    17161 tcctgttaca gttgatgtac ttagtcgttt tcgtagcaaa aaagaacaaa cagatacact
    17221 aaaaagatta tctaagggcc aagttgatat tattattgga acacatcggc ttctctctca
    17281 agatgttgta ttttcggatt tagggttaat agttattgat gaggaacagc gatttggtgt
    17341 aaagcataaa gaaaagttaa aagaattaaa aacaaaagtt gatgtcttaa ctttgacagc
    17401 gaccccaata cctagaacat tacatatgtc aatgcttggc attagagact tatcagtgat
    17461 tgaaacacct ccaaccaatc gttatcctgt gcagacttac gttttggaga caaatcctgg
    17521 tttagttaga gaagctatta ttcgtgagat agacagagga ggacaggttt tttatgttta
    17581 caataaagtt gacactattg atcagaaagt ttcagaactt caggagttag taccagaagc
    17641 tagtattggt tttgttcatg gtcaaatgtc tgaaattcaa ttagaaaata cattaataga
    17701 ctttatcaat ggagattacg atgttttagt tgcaacgaca ataattgaga caggagtcga
    17761 tatttcaaat gttaataccc tctttgttga aaatgcagat catatgggct tgtcaaccct
    17821 ttatcaatta agaggccgtg tgggtagaag taatcgcatt gcttatgctt accttatgta
    17881 cagacctgat aaagttttaa cagaaatttc tgagaaaaga ctagatgcaa tcaaagggtt
    17941 tacagaattg ggatctggtt ttaaaatagc gatgcgtgat ttatctatac gtggtgcagg
    18001 aaatatttta ggagcttcac aaagtggttt tattgattcg gttggattcg agatgtattc
    18061 acagcttcta gagcaagcga ttgccaccaa acaagggaaa tcattaattc gtcaaaaagg
    18121 taatgctgaa ttagccttac aaattgacgc ctatttacca gcagaatata tatcagatga
    18181 gcgccaaaaa atagaaatat ataagcgtat tcgtgaatta gaaactaggg ctgattatga
    18241 aaccttacaa gatgagttaa tagatcgttt tggagaatat cctgatcaag ttgcatacct
    18301 tttagaaata ggacttttaa aagcttatct ggaccttgcc tttactgaat tagtagaaag
    18361 aaaaggtaat gaaataagca tcctatttga aaaagcatct ttgaagtatt ttttaactca
    18421 agattatttt gaagcactat caaaaactca acttaaggct agaattagcg aaacaaatgg
    18481 taagatggaa gttgttttta atatcaaaca taagaaaaat tatgaaatta ttgaagaact
    18541 tctaaaattt gccgaatgtt ttatcgagat taagtcacgt aaaccagtag aagaataact
    18601 aagtgaaaag ttgcaaggaa atttggatac tcttttagaa aatgtttggt tcaagaagaa
    18661 tagtacgag
//
LOCUS       DATRNK010000043        18284 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.043, whole genome shotgun sequence.
ACCESSION   DATRNK010000043 DATRNK010000000
VERSION     DATRNK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18284
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.043"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            391..705
                     /locus_tag="VBC52_001400"
     CDS             391..705
                     /locus_tag="VBC52_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO6882981.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            721..1107
                     /locus_tag="VBC52_001401"
     CDS             721..1107
                     /locus_tag="VBC52_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO6882982.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1136..2521
                     /locus_tag="VBC52_001402"
     CDS             1136..2521
                     /locus_tag="VBC52_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO6882983.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2524..2676
                     /locus_tag="VBC52_001403"
     CDS             2524..2676
                     /locus_tag="VBC52_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6882984.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2699..3904
                     /locus_tag="VBC52_001404"
     CDS             2699..3904
                     /locus_tag="VBC52_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO6882985.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            3947..4168
                     /locus_tag="VBC52_001405"
     CDS             3947..4168
                     /locus_tag="VBC52_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6882986.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            4285..4782
                     /locus_tag="VBC52_001406"
     CDS             4285..4782
                     /locus_tag="VBC52_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEO6882987.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            4757..5263
                     /locus_tag="VBC52_001407"
     CDS             4757..5263
                     /locus_tag="VBC52_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6882988.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            5247..7694
                     /locus_tag="VBC52_001408"
     CDS             5247..7694
                     /locus_tag="VBC52_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6882989.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            7697..9874
                     /locus_tag="VBC52_001409"
     CDS             7697..9874
                     /locus_tag="VBC52_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO6882990.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            9871..10872
                     /locus_tag="VBC52_001410"
     CDS             9871..10872
                     /locus_tag="VBC52_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEO6882991.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            10869..11801
                     /locus_tag="VBC52_001411"
     CDS             10869..11801
                     /locus_tag="VBC52_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6882992.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            12046..12162
                     /locus_tag="VBC52_001412"
     CDS             12046..12162
                     /locus_tag="VBC52_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO6882993.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            12178..14097
                     /gene="tet(M)"
                     /locus_tag="VBC52_001413"
     CDS             12178..14097
                     /gene="tet(M)"
                     /locus_tag="VBC52_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO6882994.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            14216..14383
                     /locus_tag="VBC52_001414"
     CDS             14216..14383
                     /locus_tag="VBC52_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO6882995.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(14443..14796)
                     /locus_tag="VBC52_001415"
     CDS             complement(14443..14796)
                     /locus_tag="VBC52_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6882996.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            15301..15723
                     /locus_tag="VBC52_001416"
     CDS             15301..15723
                     /locus_tag="VBC52_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6882997.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            15720..15950
                     /locus_tag="VBC52_001417"
     CDS             15720..15950
                     /locus_tag="VBC52_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6882998.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            16411..16614
                     /locus_tag="VBC52_001418"
     CDS             16411..16614
                     /locus_tag="VBC52_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO6882999.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            16696..17913
                     /locus_tag="VBC52_001419"
     CDS             16696..17913
                     /locus_tag="VBC52_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO6883000.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(18105..>18284)
                     /locus_tag="VBC52_001420"
     CDS             complement(18105..>18284)
                     /locus_tag="VBC52_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEO6883001.1"
                     /translation="YAMIDSLSEEELFKPHMRKWADEATKTATWEVYKFIHVNTVAPF
                     GTFRTKIRKWKKIVL"
BASE COUNT         5987 a         3278 c         3777 g         5242 t
ORIGIN      
        1 atagtttttc tcctatcgcc ttttgtgacc atttttatga aattttttaa agttaaaaat
       61 agcataaaaa tctagttatc cgcataaaaa ctggacttat cacactttat caaggtcaaa
      121 accactcaat ttactactaa tttactactt atgaatgagc tttgatacga cgatttatcc
      181 ttgaaaagtg aagatataaa gatacttcca ataaaatttg aatatttaat aggtagacac
      241 ttcaaaaaat gaggtgtcta tttttttacc cgattttgaa aggaagtgaa cttatgaaaa
      301 caaaaaatca agaatcaaaa ggtcgttccc cactctttaa gaccatcaaa cattcattca
      361 gccaataaaa aagaaaggat aggtaaaaat atggaactta aatttgtgat tcccaacatg
      421 gaaaaaacat tcggcaattt agaatttgct ggcgaggata aagtcgttca gcgaagaatc
      481 aacggacggc taactgtctt atcaagaagc tataatctct attctgatgt tcaaagagca
      541 gatgatattg tggtggtgct tcctgctgaa gctggcgaaa aacatttcgg ctttgaggaa
      601 cgtgtgaagt tagtcaatcc acgtattacc gcagagggct acaaaatcgg cactcgtggt
      661 tttacaaatt accttttaca tgctgacgac atgataaaag aataaagaaa gagaggaaaa
      721 atgatgagat tagcaaatgg cattgtatta gataaagaca cgacttttgg agaattgaaa
      781 ttctctgctc tacgtcgtga agtgagaatc caaaatgaag acgggtcggt ttcagatgaa
      841 atcaaggaac gtacctatga cttaaaatcc aaaggacaag gacgcatgat tcaagtaagt
      901 attcctgcca gcgtgccttt gaaagagttt gattataacg cacgggtgga acttatcaat
      961 cccattgcgg acaccgttgc tactgccacc tatcaaggag cagatgttga ctggtatatc
     1021 aaggcagacg atattgtgct gacaaaggat tctagttcat tcaaagctca accacaagca
     1081 aagaaagaac cgacacaaga caaatagtcg ctaggtagaa aggagacttt ttcgcatgaa
     1141 acagcgtggt aaaaggattc gcccatctgg taaagattta gtctttcatt ttacgatagc
     1201 gtcactcctg cctgttttcc tgctggttgt cggactgttt catgtgaaga caatccagca
     1261 gatcaactgg caggatttta acctatcaca agcagataag attgacattc cctatttaat
     1321 tatcagtttc agtgtcgcaa ttcttatctg cttgctggta gcgtttgtat tcaaacgggt
     1381 tcgctatgat acggttaaac aactttacca ccgtcaaaaa ctggcaaaga tgatacttga
     1441 aaacaagtgg tatgaatctg aacaggtcaa aacagagggt ttctttaaag atagtgctgg
     1501 tcgtacaaag gaaaagataa cctacttccc taaaatgtat tatcgactta aaaatggctt
     1561 gatacagata cgggtggaaa tcacgctggg aaaatatcaa gaccaactct tacacttgga
     1621 aaagaaatta gagagtggct tgtactgtga gctgacggat aaagagttaa aggattccta
     1681 tgtggaatat actttgctct atgacaccat agccagtcgt atttctattg atgaagtaga
     1741 agctaaagat ggtaaacttc gcttaatgaa aaacgtatgg tgggaatatg ataagctccc
     1801 tcatatgttg attgctggtg gtacaggtgg cggtaaaact tactttatac tgacactgat
     1861 tgaagccttg cttcatacag attcaaaact gtatattctt gacccgaaaa atgctgatct
     1921 tgcggactta ggttctgtga tggcaaatgt ctactataga aaagaagact tgctttcttg
     1981 cattgaaaca ttctatgaag aaatgatgaa acgtagtgag gaaatgaagc agatgaagaa
     2041 ctataagact ggcaaaaatt atgcttactt aggtctcccg gcacacttct taatctttga
     2101 tgaatacgtc gctttcatgg aaatgctggg aacaaaagaa aacaccgcag ttatgaataa
     2161 gctgaaacag attgtcatgt taggtcgtca agctggcttc tttctaatac tggcttgtca
     2221 acgtccagac gcaaaatatt taggcgacgg aatccgtgat cagtttaatt tcagagtggc
     2281 tttaggtcgt atgtctgaaa tgggctatgg catgatgttt ggcagtgacg tacaaaagga
     2341 tttcttctta aagcgaatca aaggtcgtgg ctatgttgat gtaggaacaa gtgtcatatc
     2401 agagttttat actccccttg taccaaaagg atatgatttc ttggaggaaa ttaaaaagtt
     2461 atccaacagc agacagtcca cgcaggcgac gtgcgaagcg gaagtcgcag gtgtggactg
     2521 atcttgctgg ctggtgtggc aatagccacg ccagcactta accccccgta tctaacaggg
     2581 gggtacaaat cgacaggaaa cagtcaaaaa aacattagaa aatcctttgg ttacaaggga
     2641 tttacaaaat ttcagcgtat gtcaaatggg ctttaaaagt tgacatacgc ctttttgatt
     2701 ggagggattt ttactgaatg aacaaacttg gttacagcat ttaaaagaaa aacgcttggc
     2761 ttatggacta tctcaaaacc gtttagctgt tgcgactggt attacaaggc agtatctaag
     2821 cgatattgaa acaggaaaag tcaagccatc agaggattta cagcagtccc tttgggaagc
     2881 tctggaacgc ttcaatcccg acgctcccct tgaaatgctg tttgattatg taagaattcg
     2941 ctttccgaca acagacgtac agcaggtggt cgaaaacatc ttacaactga aactgtccta
     3001 ttttcttcat gaggactatg gtttctattc ttattcagag cattatgctt taggcgacat
     3061 attcgtcctt tgctcccatg aactggacaa aggagttctg gtggaattga aaggtcgtgg
     3121 gtgcagacaa tttgaaagct atcttctggc acaacaaaga agctggtatg agttctttat
     3181 ggacgttttg gtggctggcg gtgtgatgaa acgccttgac cttgccatta acgataagac
     3241 agggatttta aatatccctg tactcactga aaagtgccaa caggaagaat gtatctccgt
     3301 cttccgcagt tttaaaagct atcgcagtgg cgaactggta cgcaaagagg aaaaggaatg
     3361 tatgggaaac accctctata tcggttcatt acaaagtgaa gtttatttct gtatctatga
     3421 aaaggactac gagcagtaca agaaaaatga tattcccatt gaagacgcag aagtaaaaaa
     3481 ccgttttgag attcgattga aaaatgagcg tgcctattat gcagtccgtg atttactcgt
     3541 ctatgacaat ccagagcata ccgcctttaa aattatcaat cggtatatcc gttttgtaga
     3601 taaagacgat tccaaacctc gttctgattg gaaactgaat gaagaatggg cttggtttat
     3661 tgggaacaat cgtgaacgat taaaactaac cacaaaacca gagccttact ccttccaaag
     3721 gacgctgaac tggctatctc atcaagttgc cccgacctta aaggttgcga ttaaacttga
     3781 tgaaatcaac cagacgcagg ttgtaaaaga cattctcgac catgcgaaac tgacagaccg
     3841 acacaagcag attttgaagc aacagtcagt aaaagaacag gacgtgataa caacaaaaaa
     3901 ataactcaaa tacaaattca ttgaatatag agaggagaac atttttatga attttggaca
     3961 aaacctttat aactggtttc tatcaaacgc tcaatcactg gtgcttttag caatcgttgt
     4021 gattggcttg tatcttggct tcaagcgtga gtttagcaaa ctgattggct ttttaattat
     4081 tgcgattatt gcggttggct tagtcttcaa cgctgctgga gtaaaagaca ttttactaga
     4141 gctattcaat cgcattattg gtgcttaaat aaaaccgttc ttttgtggaa tataagtggt
     4201 tttcttatgt tccgcaaagg aatggtacac caaacgaagt gcggtaggga tttttgaatc
     4261 tctacaaaga aaggacgtga atatatggac gatatgcaag tctatattgc gaatttaggc
     4321 aaatacaatg agggcgaatt ggtcggtgcg tggtttacct ttcccattga ctttgaggaa
     4381 gtcaaagaga aaatcggctt gaatgatgaa tatgaggaat acgccattca tgactacgag
     4441 ttacccttta cggttgacga atacacttcc attggcgaac tcaatcgact atgggaaatg
     4501 gtatcggaat tacccgaaga attacaatcg gagctatctg ctctgctcac tcatttttca
     4561 agcattgaag aactaagcga acatcaagag gatattatca ttcattccga ttgtgatgat
     4621 atgtatgacg tggcacgcta ctacattgaa gaaacgggtg ctttaggcga agtaccagct
     4681 agtcttcaaa actatattga ttatcaagcc tatggtcggg atttagacct ttcaggaacg
     4741 tttatctcaa ccaatcatgg gatttttgaa atcgtctatt aaatctgtcg gtacattact
     4801 actggcagat tttctatttt acggggtggc tcaatcagct acccctattt tttatgaaag
     4861 gattgattac atgaagaaaa tacgaagcta taccagtatc tggtctgtgg aaaaggtact
     4921 gtattctatc aatgatttta gacttccgtt tcccataacc tttacgcaaa tgacatggtt
     4981 tgtcgtgtca ctctttgcag tgatgatact tggcaacttg ccccctcttt ccatgataga
     5041 gggagcattt ctcaaatact ttgggattcc tgtggctttc acatggttta tgtctacaaa
     5101 aacttttgat ggtaaaaagc cttatggatt tttgaagtct gtcattgctt atgcactgcg
     5161 accaaagctg acctatgcag gaaaaaaagt aacgcttggc agaaaccagc cacaagaagc
     5221 cattacagca gttaggagtg aattttatgg catatccaat taaatacatt gaaaacaatc
     5281 tcgtctggaa taaagacggg gaatgttatg cttactatga gcttgttcct tacaattact
     5341 catttctaag tccagaacag aaaatacaag tgcatgattc tttcagacag cttatcgcac
     5401 aaaatcgtga tggcaaaatt catgctttac aaatcagtac agaatccagc atacgttctg
     5461 cacaagagcg ttccaaaaat gaagtcactg gcaagctcaa agcggttgcc tatgacaaaa
     5521 tcgaccaaca gacagacgct ttaatatcca tgattggcga aaatcaagtg aactaccgtt
     5581 tctttatcgg ctttaagttg cttctcaacg atcaggagtt ttctatgaaa agtcttaccg
     5641 ttgaagcaaa aaatgctttg tctgattttg tctatgatgt gaaccataag ctgatgggcg
     5701 attttgttag tatgagtaat gatgaaatcc tgcgttttca gaagatggaa aagctcttag
     5761 aaaataaaat ctctcgtcgt ttcaaaatcc gcaggttaga taaggacgac ttcggctatc
     5821 tgattgaaca cctttacgga cagacaggca ctgcctatga agagtatgag taccatctat
     5881 caaagaaaaa gctggataat gaaacgctga ttaaatacta tgacttgatt aagcctactc
     5941 gctgtttggt ggaagaaaaa cagcgatatt tgaaaatcca gcaggaagat gaaaccgtct
     6001 atgtagctta ctttaccatt aacagcattg tcggagaact ggacttcccg tcctctgaaa
     6061 tcttctacta ccagcaacag caatttacat tcccgattga tacgtcaatg aatgtggaaa
     6121 ttgtagcgaa tcgtaaagcc ctatctactg tccgcaataa aaagaaagaa ctgaaagact
     6181 tggataacca cgcttggcaa agtgataatg aaaccagctc caatgtggcg gaagctctgg
     6241 aaagtgtgaa tgagctggaa accaatttag accaaagcaa ggaatctatg tacaagctgt
     6301 cttatgtggt aagggtatca gcaaatgatc ttgacgaact caaacgtcgt tgtaatgaag
     6361 tgaaagattt ttatgacgat ttaagcgtaa aactggtacg accatttggg gatatgctcg
     6421 gcttacatga agaattttta cctgccagca agcgttatat gaatgattat attcaatacg
     6481 tgacctctga tttcctcgct ggtttaggtt ttggtgctac tcaaatgctg ggggaaaatg
     6541 aggggattta tgttggctac agcttagata ctggacgcaa tgtctatctg aaacctgctc
     6601 ttgccagtca aggggttaag ggttcagtaa ccaatgcgtt agcgtcggct tttgttggtt
     6661 cgctgggtgg tggtaaatcc tttgcgaata accttatcgt ctattatgcg gtgctttatg
     6721 gggcacaagc agtgattgta gacccaaaag cagaacgtgg cagatggaaa gaaaccttgc
     6781 cagagatttc ccatgaaatc aatatcgtca ctctgacttc tgatgagaaa aacaaaggct
     6841 tacttgaccc ttatgtgatt atgaaaaatc ccaaagattc tgaatcactg gctattgata
     6901 ttctgacatt ccttacgggg atttcctctc gtgatgggga acgcttccca atccttagaa
     6961 aagccattcg tgcagtaacc aatagtgaag tacgagggtt gatgaaagtg attgaggaat
     7021 tacgggttga gaatacgcca ctaagtacca gtatagccga ccatatcgaa agttttacag
     7081 actatgactt tgcacattta ttattcagta atggttatgt ggagcagtct atcagcttag
     7141 aaaaacaact gaacattata caggttgcgg acttggtact tcccgacaag gaaacttcct
     7201 ttgaggaata taccactatg gagcttttat ccgttgctat gctgattgtc attagtacct
     7261 ttgctttaga ctttatccat acagaccgaa gcattttcaa gattgtagat ttagacgaag
     7321 catggagctt tttacaggta gcacaaggaa aaacactatc tatgaagctg gttcgggctg
     7381 gtcgtgctat gaacgctggg gtatatttcg tgacccaaaa tacagacgac ctcttagatg
     7441 aaaaactgaa aaataacctc ggcttaaaat ttgcatttcg ttccactgac cttaacgaga
     7501 ttaaaaagac cttagccttt tttggtgtag acccagagga cgaaaacaat cagaagcgat
     7561 tgcgtgattt ggaaaacggg caatgcctta tcagtgattt atatggtcgt gtcggtgtga
     7621 tacagttcca ccctgtattt gaagaactgc tccatgcctt tgataccaga ccacctgtgc
     7681 gaaaagaggt gtaaatgtga aaccatcaat agtaaacaga ataaaatcaa actggacgct
     7741 gaaacgtcta ggtaaagtgg caatgacagt ggctttcaca cttgtgattg ccatttttct
     7801 tttagccatg ctgggaacgg tggttcaagc tgcgggcttg gtagatgata cggtcaatgt
     7861 ggcaaatgaa tacagccgat acccacttga aaactatcaa ctggattttt atgtggataa
     7921 tagctggggc tggcttccgt ggaactggtc ggacgggatt ggaaaacagg tcatgtatgg
     7981 actatatgcc attaccaatt ttatttggac aatcagtttg tatgtttcca atgcgacagg
     8041 ttacttagta caggaagcct attccttaga cttcatttcc gctacagcag attccattgg
     8101 taagaatatg cagaccttag ctggtgtgag tgcaaacgga ttttcaacag agggtttcta
     8161 tgttggattc ctcttactct tgattttggt tcttggggtt tatgttgcct atacgggact
     8221 gataaagaga gaaaccacaa aggcaattca tgccattatg aattttgtgc tggtgtttat
     8281 cctatcggct tcctttattg cctacgctcc cgactacatt aaaaaaatca atgacttttc
     8341 atcagacatc agtaatgcca gtttatcact tggcacgaag attgtcatgc cccattccga
     8401 tagtcaaggc aaggacagcg tggacttaat cagagatagc ctgttttcca tacaggttca
     8461 gcaaccgtgg ctactgcttc aatacaacag ttcagacatt gaaagtatcg gtattgaccg
     8521 tgtggaaagc ctgctctcca ccagcccaga ttccaacaat ggcgaagaca gagaaaaaat
     8581 tgttgcggaa gaaattgaag acagaagcaa taccaatcta accattacaa agaccattaa
     8641 ccgtttaggt acagtcttct tcctatttgt cttcaatatt gggatttcca tatttgtatt
     8701 cctattaaca ggaatcatga ttttctcgca ggtacttttt atcatctatg ctatgtttct
     8761 gcctgtgagc tttattttaa gcatgattcc atcatttgat ggtatgtcaa aacgagccat
     8821 aacaaagctc tttaatacca ttttgacacg agctggaatc acattgatta ttacgacagc
     8881 atttagtatt tcaaccatgc tctatacctt atcggctggt tatccgttct ttttgattgc
     8941 ttttctacag attgtgacct ttgcaggaat ctacttcaag ctgggcgatt taatgagtat
     9001 gttttctcta cagagtaacg attctcaaag tgtgggaagt cgtgtgatga gaaaacctcg
     9061 tatgcttatg cacgctcaca tgcaccgtct acagcggaaa cttggacgtt ccatgactac
     9121 tctaggggct gggtctgcca ttgttacagg taaaaaagga cagtcgggtt cggggagttc
     9181 tgcaaggaca caagcagatc actcccgacc agacggaaag gaaaaatcaa cacttggaaa
     9241 acgtatcggt caaaccatcg gtacagtagc tgataccaaa gacagaatgg tagacactgc
     9301 tagtggtttg aaagaacagg ttaaagattt gccgaccaat gcaagatatg cagtatatca
     9361 aggaaaatcc aaagtaaaag agaatgtccg tgatttaacc agtagtattt ctcaaaccaa
     9421 agcggacaga gccagtggac gcaaggaaca gcaggaacaa aggcgaaaaa ccattgcgaa
     9481 gcgtcgctct gaaatggaac aggtcaaaca gaaaaaacag cctgcttctt ctgttcatga
     9541 aagaccgact acaagacaag aacaatatca tgatgaacag acctcaaaac agtctaatat
     9601 tcagacttca tataaggaat ctcaacaagc caaacaagag cgtccagcag ttaagtccga
     9661 tttttcaagt ccaaaagtgg aacgccaagg caataccgtt caagaaaaaa ccgttcaaaa
     9721 gccagcaact tcaaccacta cagcagatag aacttcacaa cgtccaatca caaaagaacg
     9781 tccgtctact gttcaaagag taccactaca aaatacaaga agtagaccac caatcaaaac
     9841 cgccaccatt aagaaagtcg gtaagaaacc atgaagttga aaactttagt gattggtggt
     9901 tctggattat tcttgatggt cttctcactg cttctgtttg ttgccatttt attttcagat
     9961 gaacaggaca gcggaatttc caatattcat tatggaggtg tgaatgtttc cgcagaagtg
    10021 ctggctcata agcctatggt agaaaaatat gccaaagaat atggcgttga agaatatgtc
    10081 aacatacttc ttgcgattat acaggtggaa tcgggcggta ctgcggaaga tgttatgcag
    10141 tcctcggaat ccctcggtct tccacctaat tcattgagta cagaagaatc cattaagcaa
    10201 ggtgtgaagt atttcagtga attattagcc agtagcgaaa ggctcagtgt agatttagaa
    10261 tcggttatcc agtcctacaa ttatggtggt ggtttcttag ggtatgtggc taatcgtgga
    10321 aataaatata cctttgaact ggctcaaagt ttctcaaaag agtattcagg tggcgaaaaa
    10381 gtgtcttacc ccaatcccat agccatacct atcaatgggg gctggcgata caactatggc
    10441 aatatgtttt atgtgcaact ggtaacgcag tatcttgtca caacagagtt tgatgatgat
    10501 acggtacaag ccatcatgga cgaagcactg aaatatgagg gctggcgata cgtttacggt
    10561 ggagcttccc cgactacttc ttttgattgt agcggactga cacaatggac gtatggaaaa
    10621 gctggaatta acttaccacg aaccgcacaa cagcaatatg atgtgaccca gcatatccca
    10681 ctatcggaag cacaagctgg cgatttggtt ttctttcatt ctacctataa cgctggctct
    10741 tatattactc atgttgggat ataccttggc aataaccgta tgtttcatgc aggcgaccca
    10801 atcggttatg ccgacttaac aagcccctac tggcaacagc atttagtggg agcaggacga
    10861 atcaaacaat gagaaaggaa gatttaatga tgaaatttag aaaaaatcag aataaagaaa
    10921 aacagatacc aaaggaaaag aaacctcgtg tctataaggt caatcctcat aaaaaggttg
    10981 tgattgcctt gtgggtactt ttagggctta gtttcagctt tgcgatattc aagcacttta
    11041 cagctataga tactcatact attcacgaaa caactatcat agaaaaggaa tacgttgata
    11101 ctcatcatgt agaaaatttt gtagagaact ttgcgaaagt ctactattca tgggagcaat
    11161 ccgataagtc cattgataat cgaatggaaa gtctaaaagg ctatctgaca gatgaacttc
    11221 aagctctcaa tgttgataca gtacgcaaag atattcctgt atcgtcttct gtaagaggat
    11281 ttcagatatg gacggtagag ccaactggcg acaatgagtt taatgtaacc tacagtgtag
    11341 accagctcat tacagaggga gaaaatacaa agaccgtcca ctctgcttat atagtgagtg
    11401 tctatgtaga tggttctgga aatatggtac tggttaagaa tccgaccatt accaacatac
    11461 ctaagaaatc aagttataaa ccaaaagcca ttgaaagtga ggggacggtt gattccatta
    11521 caaccaatga aatcaatgag tttttaacga cgttcttcaa gctctatcct acagcgacag
    11581 ccagtgaact ttcctactat gtgaatgacg ggatattaaa accaatcgga aaagagtaca
    11641 tctttcaaga actggtaaat cctattcaca atcgtaagga taatcaagtc acggtatcgc
    11701 tgacagtgga gtatatcgac cagcagacca aagcaacgca ggtatctcaa tttgatttgg
    11761 tacttgaaaa gaacgggagt aattggaaga ttatagaata acaaatattg gtacattatt
    11821 acagctattt tgtaatcacg tactctcttt gataaaaaat tggagattcc tttacaaata
    11881 tgctcttacg tgctattatt taagtatcta tttaaaagga gttaataaat atgcggcaag
    11941 gtattcttaa ataaactgtc aatttgatag tgggaacaaa taattggatg tcctttttta
    12001 ggagggctta gttttttgta cccagtttaa gaataccttt atcatgtgat tctaaagtat
    12061 ccggagaata tctgtatgct ttgtatgcct atggttatgc ataaaaatcc cagtgataaa
    12121 agtatttatc actgggattt ttatgccctt ttgggttttt gaatggagga aaatcacatg
    12181 aaaattatta atattggagt tttagctcat gttgatgcag gaaaaactac cttaacagaa
    12241 agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg
    12301 aggacggata atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc
    12361 tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc
    12421 ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca
    12481 aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt
    12541 cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat
    12601 caggatatta aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat
    12661 cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga
    12721 aacgatgacc ttttagagaa atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa
    12781 caagaggaaa gcataagatt tcagaattgt tctctgttcc ctctttatca tggaagtgca
    12841 aaaagtaata tagggattga taaccttata gaagttatta ctaataaatt ttattcatca
    12901 acacatcgag gtccgtctga actttgcgga aatgttttca aaattgaata tacaaaaaaa
    12961 agacaacgtc ttgcatatat acgcctttat agtggagtac tacatttacg agattcggtt
    13021 agagtatcag aaaaagaaaa aataaaagtt acagaaatgt atacttcaat aaatggtgaa
    13081 ttatgtaaga ttgatagagc ttattctgga gaaattgtta ttttgcaaaa tgagtttttg
    13141 aagttaaata gtgttcttgg agatacaaaa ctattgccac agagaaaaaa gattgaaaat
    13201 ccgcaccctc tactacaaac aactgttgaa ccgagtaaac ctgaacagag agaaatgttg
    13261 cttgatgccc ttttggaaat ctcagatagt gatccgcttc tacgatatta cgtggattct
    13321 acgacacatg aaattatact ttctttctta gggaaagtac aaatggaagt gattagtgca
    13381 ctgttgcaag aaaagtatca tgtggagata gaactaaaag agcctacagt catttatatg
    13441 gagagaccgt taaaaaatgc agaatatacc attcacatcg aagtgccgcc aaatcctttc
    13501 tgggcttcca ttggtttatc tgtatcaccg cttccgttgg gaagtggaat gcagtatgag
    13561 agctcggttt ctcttggata cttaaatcaa tcgtttcaaa atgcagttat ggaggggata
    13621 cgctatggct gtgaacaagg attgtatggt tggaatgtga cggactgtaa aatctgtttt
    13681 aagtatggct tatactatag ccctgttagt accccagcag attttcggat gcttgctcct
    13741 attgtattgg aacaagtctt aaaaaaagct ggaacagaat tgttagagcc atatcttagt
    13801 tttaaaattt atgcgccaca ggaatatctt tcacgagcat acaacgatgc tcctaaatat
    13861 tgtgcgaaca tcgtagacac tcaattgaaa aataatgagg tcattcttag tggagaaatc
    13921 cctgctcggt gtattcaaga atatcgtagt gatttaactt tctttacaaa tggacgtagt
    13981 gtttgtttaa cagagttaaa agggtaccat gttactaccg gtgaacctgt ttgccagccc
    14041 cgtcgtccaa atagtcggat agataaagta cgatatatgt tcaataaaat aacttagtgt
    14101 attttatgtt gttatataaa tatggtttct tgttaaataa gatgaaatat tttttaataa
    14161 agatttgaat taaagtgtaa aggaggagat agttattata aactacaagt ggatattgtg
    14221 tcctgtatgt ggaaataaaa cacgattaaa gataagggaa gatactgaat taaaaaaatt
    14281 ccccctctat tgtccgaaat gcagacaaga aaatttaatt gaaataaagc agttcaaagt
    14341 aactgtgatt acagagccag acgcaaagac gcagagccga taaaatgaga ttaatacaat
    14401 ctcattttat cggctctttc cgttatgtat ggattctttt aattagtctt cgatgtttct
    14461 tgcttcgttg ataccgctgg ctaaagattc cattaaggat agttctttgt ctgtaaagct
    14521 atccatgtat ttctctatct gtaatcgtcg ggtgcttttt accaagttat tagcaggtaa
    14581 gaaaaattca tcaacggaaa catgaagtaa cgatacaagg tcataaagaa cttgtatgct
    14641 ggggtgttgc cctttatttt caatattagt taagtaccgt gggtcaattt caatcaatgc
    14701 tcccacttgt tcacgagtta aacctcgttt caatcgagct tctttaatgg ctaaaccaaa
    14761 ggctctaaaa tcatatttat cttctttttt acgcatagta gaccacctct atacatttta
    14821 ttgttcctac tgaattaaaa acaggtatag aaaaacgtgt tatatggttt ataggtttat
    14881 atttaataaa aagcactact aaacgccaat aaaaaaaacc gttatatggt agtgctattt
    14941 acgctgttaa aatattgtat attacttcca aatggcggtt tgttggaggt caacgtcgcc
    15001 atgaagtaca tcatatacaa taaatttcct tacattgggt tcttgtcaaa aaaagtcgtc
    15061 tatctgcaat agataagtac gtccaccaat gtggttttat aaatcatata gatagaataa
    15121 cagaagcatg taaacagaga aataaatctg tttatatgct tttttggcta ttcagaactt
    15181 ttttacaaag tttatttatc agtaatgcaa caaatccccc tttcacattg ggactaagag
    15241 tgaaaggaga taaacgagca aggctcactt cctttcctag acagaaaggg ggtgagaaac
    15301 atgaaaccat cttcttttca gaccacaata gaaaatcagt ttgactatat ctgtaaacgt
    15361 gctatggaag acgagcgaaa gaattatatg ctttatcttt caaggattgc aaagcgtgag
    15421 gtgtcctttt cggatgttgg cgattatctt gttagccagt ttgcgacaac agataactat
    15481 tcaactgact ttcagatttt tacactcaat gggttatcag taggcgttga aaatgatttg
    15541 ttgagtgaag cattacgtga gttgccagac aagaaacgtg aaattctact gctgttttac
    15601 tttatggaca tgagcgattc agaaattgca gacctgttga aattgaaccg ttctactgtc
    15661 tatcggcata gaaccagtgg actagcctta attaaaaagt ttatggagga atttgaagaa
    15721 tgaaaacaca atatcctatg attccctttc ctctcattgt aaaggcaaca gatggcgata
    15781 ccgaagcgat taaccagatt ctacatcatt acagagggta cataacgaag cgttccctac
    15841 gacttatgaa agatgaatat ggcaatcaaa gtatggtcgt tgatgaagtc ttacgtggaa
    15901 gaatggaaac cagactgatt acaaagattt tgtcatttga aattaagtaa tatcctctct
    15961 cctttcgtgg aagcgtgcta aaccattcca cgcttcccga acagggaggt ttgttattcc
    16021 accaaagcat attgagcttt caatgtgttt tgataggcta acgagccatt gttctttgaa
    16081 aactgaataa aagtaatcga atacgtttcg ataagaaaag agccaacgga actaaccgcc
    16141 atgacctatc ttataaagat agcgagcgat tcatgttagt gatccgagaa gcaatcttta
    16201 gcaggattgc ctgcaacgac attcttatcg tgataatgat actcccatac agtcaatagt
    16261 ccgagcgtga taaaaccgtc gcaggcaatg agtatggcta catgagaacc atgcaggggt
    16321 ggaactcccg tgagctttgc taaagctgtt cgattgctgg taaaacaact tttatgaaat
    16381 ccaaataagt gatttggaaa ggaggatttt atgaagcaga ctgacattcc tatttgggaa
    16441 cgttataccc taaccattga agaagcgtca aaatattttc gtattggcga aaacaagcta
    16501 cgacgcttgg cagaggaaaa taaaaatgca aattggctga ttatgaatgg caatcgtatt
    16561 cagattaaac gaaaacaatt tgaaaaaatt atagatacat tggacgcaat ctagcgtcgc
    16621 caaagggtct tgtatatgat aaaatagtat taagtcgtat caaggctctt tccataaagg
    16681 aaaggagcaa atgccatgtc agaaaaaaga cgtgacaata aaggtcgaat cttaaagact
    16741 ggagagagcc aacgaaaaga cggaagatac ttatacaaat atatagattc atttggagaa
    16801 ccgcaatttg tttactcgtg gaaacttgtg gctacagacc gagtaccagc aggaaagcgt
    16861 gattgtatct cacttagaga gaaaatcgca gagttacaga aagacattca tgatggtatt
    16921 gatgttgtag gaaagaaaat gacactctgc cagctttacg caaaacagaa cgctcaaaga
    16981 ccaaaggtta gaaaaaacac tgaaactgga cgcaaatatc ttatggatat tttgaagaaa
    17041 gacaagttag gtgtaagaag tattgacagt attaagccat cagacgctaa agaatgggct
    17101 attagaatga gtgaaaatgg ttatgcttat caaaccatca ataactacaa acgttcttta
    17161 aaggcttcat tctatattgc tatacaagat gattgtgttc ggaagaatcc atttgacttt
    17221 caactgaaag cagttcttga tgatgatact gtccctaaga ccgtactaac agaagaacag
    17281 gaagaaaaac tgttagcctt tgcaaaagct gataaaacct acagcaaaaa ttatgatgaa
    17341 attctgatac tcttaaaaac aggtcttcgt atttcagagt ttggtggttt gacacttcca
    17401 gatttagatt ttgagaatcg tcttgtcaat atagaccatc agctattgag agatactgaa
    17461 attgggtact acattgaaac accaaagacc aaaagtggcg aacgtcaagt tcctatggtt
    17521 gaagaagcct atcaagcatt taagcgagtg ttagcgaatc gaaagaatga taagcgtgtt
    17581 gagattgatg gatatagtga tttcctcttt cttaatagaa agaactatcc aaaagtggca
    17641 agtgattaca acggcatgat gaaaggtctt gttaagaaat acaataagta taacgaggat
    17701 aaattgccac acatcactcc acatagtttg cgacatacat tctgtaccaa ctatgcaaat
    17761 gcaggaatga atccaaaggc attacagtac attatgggac atgctaatat agccatgacg
    17821 ctgaactatt acgcacatgc aacattcgat tctgcaatgg cagaaatgaa acgcttgaat
    17881 aaagagaagc aacaggagcg tcttgttgct tagtagtaca aatgaattta ctacttattt
    17941 accacttctg acagctaaga catgaggaaa tatgcaaaga aacgtgaagt atcttcctac
    18001 agtaaaaata ctcgaaagca catagaataa ggctttacga gcatttaaga aaatataaaa
    18061 agataattag aaatttatac tttgtttatg taaaaaaata taattcataa cactattttt
    18121 ttccattttc tgattttagt tctaaaagtt ccaaaaggtg caactgtatt tacatgaata
    18181 aacttatata cttcccaagt ggcggtttta gtcgcttcat cagcccattt tctcatatgt
    18241 ggtttaaata attcttcttc actcaacgaa tcaatcattg cata
//
LOCUS       DATRNK010000044        17931 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.044, whole genome shotgun sequence.
ACCESSION   DATRNK010000044 DATRNK010000000
VERSION     DATRNK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17931
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.044"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            18..1757
                     /locus_tag="VBC52_001421"
     CDS             18..1757
                     /locus_tag="VBC52_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6883002.1"
                     /translation="MTLFLIMMERAGLIILLAYAFVHIPFIKQTLKQPELKKHQYILL
                     ILFSLFAIISNFTGVEIQSDLSIIPQTLNHIADQSSVANTRVLTIGVSGLIGGPIVGI
                     IVGLLSVFVRYLQGGLAPHIYVISSLLIGLCSGLSGNYLRRNYNKIRVLDAMVVGFGM
                     EILQMICILIFSVDFNQALRLVSFISMPMILSNTLGLGIFISIISSTQKLEEHAKAFQ
                     THQVLELANLTLPYLRKGLTTESCQPVAEIIHKHMDVSAVSLTSQSAILAYVGDGADH
                     HLPNTQILTKLAKRAIDTGKVSVATDKSEIECDHKNCPLSSAIVIPLHIHDVIVGTLK
                     LYFSDAQHMTYVDRQLAEGLGNIFSTQLALGQAEEATRLLQDAEMKSLQAQVNPHFLF
                     NALNTIYGLIRMDSEKARKLVQDFSKVIRANLQRAKQNLIPLHDELEQVNAYLALEEA
                     RFPNMVAFNLDNQTNSDDNLMIPPFTLQVLIENSYKHAFKHVNKNNQLKVTIARNNDR
                     LHIIVQDNGIGIPKEKLITLGKKTQISKQGSGTAIENLVRRLNIIYDGQASLKFESND
                     SGTCAIVNIPIKN"
     gene            1769..2503
                     /gene="ltdR"
                     /locus_tag="VBC52_001422"
     CDS             1769..2503
                     /gene="ltdR"
                     /locus_tag="VBC52_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LtdR"
                     /protein_id="HEO6883003.1"
                     /translation="MKVLVVDDEPVARNELIYLLNKYDSNLVIAEAHDMATALAILLR
                     ETFDVALLDIHLRDDSGLQLAEYINKMPKPPLLIFATAYDQYAIQAFEHDARDYLLKP
                     YDFDRLKQAMDRVKGALSTSTIIESVTSGPLFKQQYPLTVEDRIYLVSADDILLIEAM
                     QGKLIIQTPDKNYEIDGSLQQWQDKLPSSQFVRVHRSYIVNINAIKTIEPWFNQTLQL
                     HLCNKITVPVSRANVKPLKQMLGIST"
     gene            2659..4143
                     /locus_tag="VBC52_001423"
     CDS             2659..4143
                     /locus_tag="VBC52_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEO6883004.1"
                     /translation="MVTFLGGVALLIVGYFTYGRYIEKNFQIDENRQTPAEALRDGYD
                     FVPMPKWKNGMIELLNIAGTGPIFGPILGALYGPVAYIWIVLGCIFAGAVHDYMIGMI
                     SLRNNGAYLPELASRYLGKSMKHVINIFSMLLLILVATVFVVTPANLILSILPAGTLS
                     LPWIIGLIFVYYLISTVLPIDKALGKVYPVFCVILMVSTAAVGFRLLTGGFDMPNLTF
                     ETFKNMHPAGLGIFPALFFTISCGAISGFHATQAPMVSRTTVNEREGRFTFYGMMIAE
                     GVIAMIWAGASMSLFKGQNLYEMIAAGTPSAVVNQVMLMLLGSVIGTIAIIGVIVLPV
                     SSGLSAFRSLRTIVADYIHVKQDTLPKIFAVTIPLYVISFVLTHVDFNLLWRYFNWAN
                     QVTAVIGLLVATRYLILKRRNYWVTFVPAMFMLYAVVVYILSQPIGFNMGLGILTYSL
                     ALVLTGILVGLFWKSGQKQLKTVHPEAFLFNDHRPINYYSSLDS"
     gene            4344..4916
                     /locus_tag="VBC52_001424"
     CDS             4344..4916
                     /locus_tag="VBC52_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEO6883005.1"
                     /translation="MRKRSILPILAVLFVFMSIFSVYSIFRGDKAKEKASVIKQASQT
                     SQTSKKEVLQKKTYPNLNKYSNLEIHVSSTRQTMTITSNDKVIFKTIVSTGAKESPTP
                     KGTFVIEPERGDFFYNASSKEGAYYWVSFKEHGIYLFHSVPTDQQGNEIPEEAKQLGK
                     AASHGCVRMSRADAKWFYENIPQGTTVTIK"
     gene            5192..6043
                     /gene="ylqF"
                     /locus_tag="VBC52_001425"
     CDS             5192..6043
                     /gene="ylqF"
                     /locus_tag="VBC52_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEO6883006.1"
                     /translation="MATIQWFPGHMSKARRQVQENIKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADPIRTKEWRDFYESQGLKTLAINSKEQSTVKKVTDIAK
                     ILMSDKIAKLRGRGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFKTYY
                     PDRLKERFKSINLEDEAPEIIMALTQKLGYRDDYDRFYNLFVKEVRDGKLGRYTLDIV
                     GEHDGNN"
     gene            6030..6791
                     /locus_tag="VBC52_001426"
     CDS             6030..6791
                     /locus_tag="VBC52_001426"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEO6883007.1"
                     /translation="MATIKEIKAILETIVDLKDKRWQEYQTDSRAGVQKAILQRKKNI
                     QSDLDEEARLEQMLVYEKKLYIEHINLIAGIDEVGRGPLAGPVVAAAVILPPNCKIKH
                     LNDSKKIPKKKHQEIYQNILDQALAVGIGIQDSQCIDDINIYEATKHAMIDAVSHLSV
                     APEHLLIDAMVLDLSIPQTKIIKGDANSLSIAAASIVAKVTRDKIMSDYDSTYPGYAF
                     SKNAGYGTKEHLEGLQKYGITPIHRKSFEPIKSML"
     gene            6812..7360
                     /locus_tag="VBC52_001427"
     CDS             6812..7360
                     /locus_tag="VBC52_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEO6883008.1"
                     /translation="MTEKEKMLAGQYYRPSAPELRKDREVALKNMQAFNNEDNSSKRN
                     VILQKWFGATGKSIHMEQRFVCDYGCNIYVGENFYANFNQTFLDVCEIRIGDNCMFAP
                     NCQLLTPLHPLDPIERNSGLEYGAPIQIGNNVWLGGGVTILPGVVLGDNVVVGAGSVV
                     TKSFENNVVIAGNPAKIIKKLA"
     gene            7598..8560
                     /locus_tag="VBC52_001428"
     CDS             7598..8560
                     /locus_tag="VBC52_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO6883009.1"
                     /translation="MKKLLTTPVMGCTLVILSLTSLFVGVKSIPLEQITHLDQSQVDI
                     FLTSRLPRTISILISGASLSVCGLLMQQLTQNKFVSPTTSGTMDWAKLGVVVTLIFFK
                     NTSIFIQLCIASGFAILGSLLFVTILKMITFKDNIFIPLIGLMLGQIVAAATVFLGTH
                     FQVLQSVNSWLQGNFSIMTSHRYEILYLALPCLFLVYFFAHQFTIVGLGESFAKNLGV
                     AYEKMIYFGLVLVSIMTSLVIIIVGALPFLGLIVPNLISITKGDHMSSTILETSLLGA
                     CIVMICDLFGRLVIFPYEVSIGVTLGVLGSAFFLISIIRNEGKS"
     gene            8557..9531
                     /locus_tag="VBC52_001429"
     CDS             8557..9531
                     /locus_tag="VBC52_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO6883010.1"
                     /translation="MIKDLEIPKLLILLILLIAAIILFLIYGIPTDANEFLIIYILKT
                     RYQKLIALILVGICIGSSSLIFQTLTNNRLLTPSIIGLDSLYILIQTGLMYLIGAQRV
                     IKFSSFSSFLLSLLLMVGFAYLLFTILFRNKKQSLYFVLLAGLIFNTLFSSISSFIQA
                     IMDPNDFMILQNQLFASFNAINTKILWISFIIIVVSFVINWPFIKELDVLLLGKENAI
                     SLGISYQKLTTRFFLWLALMVAIATALVGPITFLGLLVAHITYHSFHTFRHQILVPIA
                     IVICIFTLVLGQHLVQNLLHLTVQLSVLLNLIGGSYFIFTLIKGRKNL"
     gene            9528..10289
                     /locus_tag="VBC52_001430"
     CDS             9528..10289
                     /locus_tag="VBC52_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6883011.1"
                     /translation="MIQINNLHKFYGQKEILKDINISIPKGKVTAILGPNGSGKSTLL
                     SCISRLEPYDNGEIFLDKVPLAHYSSNDLAKTLAILRQSNHLTLKIKVRDLIGFGRFP
                     YSKGRLSQKDKAVIESVISYMDLNDIADEFINNLSGGQIQRAFIAMTMAQDTQYICLD
                     EPLNNLDMKYAVQMMDLIKRYAYEFNKTIVIIIHDINFATHYADNVVALKEGQVVTCG
                     TVEDVMQEKILSHLFDMPIRIETVDGKPIILFCTT"
     gene            10351..11379
                     /locus_tag="VBC52_001431"
     CDS             10351..11379
                     /locus_tag="VBC52_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6883012.1"
                     /translation="MTKKLIIAILALCTILTTSQAVLAKEKSQTVTIKNNYSVYIKKE
                     KRDKPDNKKQISETLKVPLKPKKVVVFDMGALDTITALGAEKSVIGIPKAKNALSLLP
                     NNVKSVYKAKRYQDVGSLFEPNFEAIARMQPDVVFLGARMASVDNIEKLKEAAPKAAL
                     VYAGVDSKKVFDKGVAERVTMLGKIFDQNKKAKTFNKDIAQAVLKLQKTIEKKGKPTA
                     LFVMANSGELLTQSPSGRFGWIFSVGGFKAVNENEKLSSHGTPVSYEYIAEKNPNYLF
                     VLDRGATIGQGASSKELFNNDVIKATDAVKNKRVHEVDGKDWYINSGGSRVTLRMIKD
                     VQNFVDNR"
     gene            11517..12359
                     /gene="dprA"
                     /locus_tag="VBC52_001432"
     CDS             11517..12359
                     /gene="dprA"
                     /locus_tag="VBC52_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEO6883013.1"
                     /translation="MNHFELFKLKKAGLTNLNINNIINYLKKNSLTSLSVRNMAVVSK
                     CKNPTFFIENYKQLDLKKLRQEFKKFPVLSILDSNYPLELKEIYNPPVLLFYQGNIEL
                     LSKPKLAVVGARQASQIGCQSVKKIIKETNNQFVIVSGLARGIDTAAHVSALKNGGSS
                     IAVIGSGLDVYYPTENKKLQEYMSYNHLVLSEYFTGEQPLKFHFPERNRIIAGLCQGI
                     VVAEAKMRSGSLITCERALEEGREVFAIPGNIIDGKSDGCHHLIQEGAKCIISGKDIL
                     SEYQ"
     gene            12454..14574
                     /gene="topA"
                     /locus_tag="VBC52_001433"
     CDS             12454..14574
                     /gene="topA"
                     /locus_tag="VBC52_001433"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEO6883014.1"
                     /translation="MATTTKTSTKKTSKKKSATAKKNLVIVESPAKAKTIEKYLGRNY
                     KVVASVGHIRDLKKSSMSIDFENNYEPQYINIRGKGPLINDLKKEAKKAKKVYLASDP
                     DREGEAISWHLAHILDLDKEDRNRVVFNEITKDAVKNAFVEPRQINMDLVDAQQARRV
                     LDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENEIKAFQPEEYWTIDGSFKK
                     GTRKFNATFYGLDGKKFKLSNNEDVKTVLKRIKTDEFLVEKVEKKERRRNAPLPYTTS
                     SLQQDAANKINFRTRKTMMIAQQLYEGLSLGTAGHQGLITYMRTDSTRISPLAQNEAT
                     EFITNRFGANYSKHGNKVKNASGAQDAHEAIRPSSVNHTPESIAKYLDKDQLKLYTLI
                     WNRFIASQMTAAVFDTMKVNLTQNGVTFIANGSQVKFDGYMAVYNDTDKNKMLPDMEE
                     GESVKKVNTNPEQHFTQPPARFSEASLIKTLEENGVGRPSTYAPTLETIQKRYYVKLA
                     AKRFEPTELGEIVNSLIVEFFPDIVDVTFTAEMEGKLDEVEIGKEQWQKIIDEFYKPF
                     EKELAKAETEMEKIQIKDEPAGFDCELCGSPMVIKLGRYGKFYACSNFPECHNTKAIT
                     KEIGVICPICQKGQVIERKTKRNRIFYGCDRYPECEFTSWDKPIGRTCPKSNDFLVEK
                     KVRGGGKQVVCSNEKCDYQEEKIK"
     gene            14711..15412
                     /locus_tag="VBC52_001434"
     CDS             14711..15412
                     /locus_tag="VBC52_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6883015.1"
                     /translation="MSYIEMKHSYKRYQTGETEIVANNDISFSIERGELVVILGASGA
                     GKSTVLNILGGMDSNSEGEVLIDGKNIANYTIRELTRYRRYDVGFVFQFYNLVPNLTA
                     LENVELASEIVPKALDAQQALENVGLGHRINHFPAQLSGGEQQRVAIARAIAKKPKLL
                     LCDEPTGALDYQTGKQVLAILQKMAQSKETTVIIVTHNTALAPIANRVIHMHDSKISD
                     IVINENPSDIQNIEY"
     gene            15424..>17931
                     /locus_tag="VBC52_001435"
     CDS             15424..>17931
                     /locus_tag="VBC52_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6883016.1"
                     /translation="MGKTFWKDIYRSITTSKGRFSSILLLMMLGSFAFIGLKVSAPNM
                     QRTAQNYLAHHHVMDITVFNSWGLDKHDQTVLESLKGSQVEFSYFVDTTPQQNSKSYR
                     LYSNTKTISTFDLVKGRLPLNKSEIALSFQERKKYAIGDKINFKQDKNKLFSNTGPLT
                     IVGFVNSTEIWSKTNLGSSQTGDGDLDSYGVLDKTAFHSPVYTMARVTFKDLRLINPF
                     SISYKEKVAKYQEKVSRKLNIHNKIRYTKTKKESLRKIDEEEKSLLKAQKQINRLDND
                     SLAMPLSQRQAIQMKIKQDRLSLLKRTKELLKLRHNTQIMESPQIIVYNRTTFPGGQG
                     YNTFDSSTNSTSKISNLFPIILYLVAALVTLTTMTRFVEEERTNAGILKALGYSDRQV
                     IFKFIIYGFIAGTLGTTLGIIGGHYLLPRIISDIISKDLTIPNTQYHLFLNYSLLAFV
                     FSLLSIVLPVFVITRRELKEKAAFLLLPKPPAKGSKIALEYINWIWKKLSFTQKVTAR
                     NIFRYKQRMIMTIFGVAGSVALLFSGLGIQSSLKQTVNEHFGRIMPYDILLTYNTNAS
                     PPKILELLSKDSKIDKYQPIHLENLDESIPGQINKQSISLFITDKKQLLPFIYLQEAT
                     TNKSLHLNNKGIIISKKLAQFYHVNTGDFIHLSHSQTLPSRKLKITGVVNANVGHYIF
                     MTKQYYRTIFKKEAKDNAFLVKLTKHKIANNLAEKLLEINGVESLTQNALQLASVEAV
                     VRSLDGSMTILVVVSLLLAIVILYNLTNINLAERKRELSTIKVLGFYNEEVTLYIYRE
                     TIILSTIGVILGTISGTYLHRQMMLLIGSDQILFGEKV"
BASE COUNT         6081 a         3010 c         3125 g         5715 t
ORIGIN      
        1 tactttggag agatttaatg acactctttt taattatgat ggagcgagcg gggttaatca
       61 tattattagc ctatgccttt gtgcatatcc cctttataaa acaaactttg aagcaacccg
      121 aattaaaaaa acaccagtat attctgctta tcttatttag cttatttgct attatttcta
      181 attttacagg ggtagaaatt cagtcagatt tatctatcat tccacaaact cttaatcata
      241 tagcagatca atcatcggtt gccaacacac gtgttttaac cattggtgta tcaggtctaa
      301 tcggtggccc catagtcggt attatagttg gtctcttatc tgtattcgtt aggtacttgc
      361 aaggaggatt agcccctcat atttatgtca tttcttcctt actaattggt ttatgctctg
      421 ggttatcagg caattatctt cgtagaaatt ataataaaat tcgtgtttta gatgcaatgg
      481 ttgttggttt cggtatggaa attctacaaa tgatctgtat cctcattttt agtgttgact
      541 ttaatcaggc gcttcgatta gtatctttta tcagcatgcc aatgatttta agtaatactt
      601 taggtttagg tatatttata tccattattt catctactca aaaattagaa gaacatgcta
      661 aagcatttca aacccatcaa gtacttgaat tagcaaattt aacccttcct taccttagaa
      721 agggactgac aacagaatct tgtcaaccag tagctgaaat tattcataaa catatggatg
      781 tgagtgctgt tagcctgact agtcaatcag ccattttagc ctatgtagga gatggagctg
      841 accatcatct tcctaatact caaatattaa caaagcttgc taaaagagca atagatactg
      901 gtaaagttag tgtcgcaact gataaaagtg aaatcgaatg cgaccataag aattgcccac
      961 ttagttcagc aattgttatt ccattacaca tccacgatgt tatagtgggt actttgaaat
     1021 tatatttttc agacgcacaa catatgactt acgttgaccg tcaattagct gaaggattag
     1081 gcaatatctt ttccactcag ttagctttgg gacaagctga ggaagcaaca aggttactac
     1141 aggatgcgga aatgaagtct ctccaagcgc aggttaatcc gcattttctt tttaatgcac
     1201 ttaatactat ttatggttta attcgcatgg atagcgaaaa agcaagaaag ctcgtccaag
     1261 attttagcaa ggttattcgt gctaatcttc aacgggcaaa gcaaaattta attcctcttc
     1321 atgatgaatt ggaacaggtt aacgcctatt tagcccttga agaagcaaga tttcccaaca
     1381 tggtggcctt taatttagac aaccaaacaa attcagatga caacttaatg attcctccct
     1441 ttacattgca ggtcttgatt gaaaactctt ataaacacgc ttttaagcat gttaataaaa
     1501 ataaccagct aaaggttact attgcaagaa ataatgatag gcttcatatc attgtccaag
     1561 acaatggtat tgggattcca aaagaaaaat taataacgct tggaaaaaaa actcaaattt
     1621 caaaacaagg ttctggtaca gctattgaga atctcgttcg tcgcttgaat attatttatg
     1681 atggacaagc aagtttaaaa tttgaatcaa atgattcagg aacttgtgct attgttaata
     1741 tccccattaa aaattagaaa ggtattgtat gaaagtttta gtagttgatg atgaaccagt
     1801 tgcacgtaac gaattaattt accttcttaa taagtatgat tctaacctcg ttatagcaga
     1861 ggcgcatgat atggctactg cattagctat tttacttaga gaaacttttg atgtagcact
     1921 gttagatatc catctcagag atgattctgg gttgcaatta gcagagtata tcaataaaat
     1981 gcccaaacca ccattattga tatttgcgac tgcttatgat caatatgcta ttcaggcttt
     2041 tgagcatgat gcgcgtgatt atttgttaaa accctatgat tttgataggc taaagcaagc
     2101 tatggataga gtaaaaggag cgctaagtac atctacaatt atagagagcg taacttccgg
     2161 tcctctcttc aagcaacagt atccattgac agtagaagat cgaatctatc tggtgtcggc
     2221 ggatgatatc cttttgattg aagctatgca aggaaaactg attatacaaa cacctgataa
     2281 aaattatgaa attgatggct ctctacaaca atggcaagat aaactaccat catctcaatt
     2341 tgtacgggta catcgctctt acattgtgaa cattaatgct attaaaacga ttgaaccttg
     2401 gtttaaccaa acacttcagt tacacctttg taataaaata acagttcctg ttagcagagc
     2461 aaatgtaaaa cccctaaaac aaatgttagg catatctacc taactaatga aaagttattc
     2521 atcataaaac ttagactatt catttctaaa aaacaaccat tcatcttgaa aataggaaat
     2581 ttcaattttt caatgctata ctaaagtcat gaaaacgctt aataaaatta agcaaaaaaa
     2641 tttttaggag gcagtgatat ggtaacattt ttaggagggg tagccctttt aattgttggt
     2701 tatttcacgt acggtagata cattgaaaag aattttcaaa ttgatgaaaa tcgccaaaca
     2761 cctgcagaag cattaaggga tggatatgat tttgttccta tgccaaaatg gaagaatggg
     2821 atgattgagt tattgaacat cgcaggaacc ggacctatct ttggacctat tttaggagca
     2881 ctctatggtc cagttgctta tatctggata gtcttaggat gtatttttgc aggggctgtt
     2941 catgattata tgattggaat gatttctctt agaaataatg gtgcatatct tcctgaatta
     3001 gctagtcgct atcttggaaa gtcaatgaag catgtgatca acattttttc aatgctatta
     3061 cttattttag ttgcaaccgt atttgtggta actccagcta atcttatcct aagtatttta
     3121 ccagctggga cactatcttt accttggata atcggtctta ttttcgttta ttaccttatt
     3181 tctacagtct tacctattga taaagcactt ggtaaagttt atcctgtatt ctgcgtcatc
     3241 ttaatggtta gtacggcagc agttggcttc cgtttattaa ctggtggctt tgatatgcca
     3301 aacttaactt ttgaaacctt taaaaatatg catccagctg ggcttgggat attccctgct
     3361 ttattcttta ccatttcatg tggagccatc tctggtttcc atgctaccca agcacctatg
     3421 gtctcaagaa caactgttaa tgagagagaa gggcgtttca ctttctacgg catgatgatt
     3481 gcagaaggag taattgcgat gatttgggca ggtgcttcaa tgagcttgtt caaaggtcaa
     3541 aatctctatg aaatgattgc tgctgggaca ccttctgcag ttgtcaatca agtaatgtta
     3601 atgttactcg gtagtgtaat tggaactatc gctatcattg gtgttattgt tctccctgtt
     3661 tcatctggtt tatcagcttt ccgtagctta cgtactatcg ttgctgatta tatccatgtc
     3721 aaacaagata ctcttccaaa aatctttgct gtaactatcc cattatatgt tatttcattt
     3781 gtccttacac acgttgattt taatctccta tggcgttact ttaactgggc aaatcaagta
     3841 actgctgtta ttggtttatt agttgctaca agatatctca tcctaaaaag acgaaattat
     3901 tgggtaacat ttgtaccagc tatgttcatg ttgtatgcag tagttgttta tattttatct
     3961 caaccaattg gatttaatat gggcttaggc atcttaactt attcacttgc tttagtgtta
     4021 acaggtattc ttgttggact tttctggaaa tctggtcaaa aacaattaaa aactgttcat
     4081 cctgaagcat tcctgtttaa cgatcataga ccaattaatt attacagtag tttagattct
     4141 taatattatt ttgaagccat gttattaaga cttacagtta tcacaataac ctcgaatgaa
     4201 gtatcattcg aggttatttt tctactaaaa attccagtaa atatttctgt ttgattacaa
     4261 atattacaaa aatatttgac aaccttcttt cagaaactta taatagattt gtccataaca
     4321 gagcaaaaga gagaggatag tacatgagaa aacgttcaat tttgccaata ttagctgtct
     4381 tatttgtttt catgagtatc tttagcgtat attctatttt tcgtggtgat aaagccaaag
     4441 aaaaagcaag tgtcataaaa caagcatctc aaacaagtca aacttcaaaa aaagaggtct
     4501 tacaaaagaa gacttatcca aatctaaata aatattcaaa tctagaaatt catgtttcat
     4561 ctacgaggca aacaatgaca ataacctcga atgacaaggt catctttaaa acgattgttt
     4621 caacaggagc aaaagagagc cctaccccaa aaggaacttt tgtcatagaa ccggaacgag
     4681 gagatttttt ctataacgcc tcatcaaaag aaggagccta ttattgggtc tcctttaaag
     4741 aacatggtat ttatctcttt catagcgttc cgactgatca acaaggaaat gagattccag
     4801 aagaagcaaa gcaattagga aaagccgcct cacatggctg tgtgcgtatg tcaagggcgg
     4861 atgcaaaatg gttctatgaa aatattcctc aaggaacgac tgttaccatc aaatagtctt
     4921 aatgtggtaa aaatcatgtt attaaaagct cggagtaaac ttccagagct ttttcgtata
     4981 tcccttaaac tcaattagta aacgaaacct atcagcggaa taaatgtata aagtatttcg
     5041 atatcattaa aaaaatgata gaataatagt gttaatcgaa tattttatcg acctttatat
     5101 gcggttgaaa aagaataaaa ctaaaaaatt agacctctaa caaatagatg tagactttta
     5161 aggtagtcta tacataagga gaatttattt catggctact attcaatggt tcccaggaca
     5221 catgtccaaa gctcgccgtc aagttcaaga aaatattaag catgttgatt ttgttacaat
     5281 tttagtggat gcaaggttac ctctttctag tcaaaatcca atgctaacca aaatcgtcgg
     5341 agacaaaccc aaactaatga tcctcaataa agctgatcta gcagatccta ttcgaactaa
     5401 agaatggaga gatttttatg aatctcaagg tttaaagaca ttagctatta attctaaaga
     5461 acaatcaaca gttaaaaaag taaccgacat cgctaaaata ttaatgtccg ataaaattgc
     5521 taagctacgg ggacgtggta ttcaaaaaga gacacttcgt acgatgatta ttggaattcc
     5581 gaatgcaggt aaatcgactc taatgaatcg cctagcaggt aaaaaaattg cagtggttgg
     5641 taataaacca ggtgtcacaa aaggtcagca atggctcaaa tctaataagg aactggaaat
     5701 tttggacact cctggtatct tgtggcccaa atttgaagat gaattagttg gattaaagct
     5761 agcattaaca ggggcaatca aagatcaatt attgcctatg gatgaggtaa caatatttgg
     5821 attgaattac tttaaaacct attatcctga tcgtctcaaa gagcgtttta aaagcattaa
     5881 tttagaggat gaagctcctg aaatcatcat ggccttaaca caaaaattag gttatcgtga
     5941 cgattacgat cgcttttata atttatttgt caaagaagtt cgcgatggta aattaggacg
     6001 ctatacgtta gatatcgtcg gagaacatga tggcaacaat taaagaaatc aaagcaattt
     6061 tagagactat tgttgattta aaagacaaac gttggcaaga atatcaaaca gattcacgcg
     6121 caggtgttca aaaagcaatc cttcaacgaa aaaaaaatat tcagtcagat ttagacgaag
     6181 aagcgcgctt agaacaaatg ttggtctacg aaaaaaaact ttacattgaa catataaacc
     6241 ttattgcagg aatcgatgaa gtaggtagag gtcctttagc gggaccagta gtagctgctg
     6301 ctgttattct tccaccaaac tgtaaaataa aacatctcaa tgactccaaa aaaattccaa
     6361 agaaaaagca tcaagaaatc tatcaaaata ttttagatca agcgttagca gttggtattg
     6421 gtattcaaga cagccaatgt attgatgata tcaacattta tgaagctaca aaacatgcta
     6481 tgattgatgc agtttctcat ttatctgtgg ctcctgaaca tcttttaatt gatgctatgg
     6541 ttttagatct atcaattcca caaactaaaa ttatcaaagg tgatgctaat tcactctcta
     6601 ttgcagcagc ttcaatcgta gctaaagtga ccagagataa aataatgtct gattatgata
     6661 gcacatatcc tggatatgca ttttctaaaa atgctggcta tggtactaaa gagcatttag
     6721 aaggtttgca aaaatacggt attactccga tacatcgtaa aagttttgaa cctataaaat
     6781 caatgttgta aataatacgg aagaggaaaa gatgaccgaa aaagaaaaaa tgttagcagg
     6841 acaatattat cgtccatcag cacctgaact acgcaaggat agagaagttg cactaaaaaa
     6901 tatgcaagcc tttaacaatg aagataatag tagcaaacgt aacgtcattt tacaaaaatg
     6961 gtttggtgct actgggaaga gtatccatat ggaacaacgt tttgtctgcg attatggttg
     7021 taatatttat gttggtgaaa acttctacgc caattttaac caaactttct tagatgtttg
     7081 tgaaattcgt attggcgata actgtatgtt tgccccaaac tgtcaactcc tcaccccact
     7141 ccatcctcta gatccaatag aaagaaattc aggattagaa tacggtgccc caatccaaat
     7201 cgggaataat gtctggctag gtggaggagt gactatttta ccaggtgttg tattaggaga
     7261 taatgtcgtc gttggcgctg gaagtgtcgt tacgaaatca tttgaaaata acgttgttat
     7321 agcaggaaac cctgctaaga taatcaaaaa attagcttaa aattagaaaa acctttaatt
     7381 ataacgaagg ttttttctaa tttcaaaaat aggtaaagta aatattctgc taatttaatc
     7441 tatcttattt ataatgattt taaatagaaa aaccttgata taataatgtt ttcttaagta
     7501 taaaattctt taaaaagtag atatttttcc aaaaaattat gttattatag ttttgtgctc
     7561 atttttgaac actttatctt tcattttaag gagttttatg aaaaagctat taaccactcc
     7621 tgtaatgggt tgtactttgg ttatactttc tctaacgtcc ctatttgttg gtgtgaaatc
     7681 tatcccactg gagcaaataa cacaccttga tcaatcacag gtagatattt ttttaacgag
     7741 tcgcttacca agaaccatta gtatcctaat atctggtgca agtttgtcag tctgtggcct
     7801 gctgatgcaa caactaaccc aaaacaagtt cgtttcaccg acaacttcag ggactatgga
     7861 ttgggcgaaa ttaggagtag ttgttacgct gattttcttt aaaaatacct ctatttttat
     7921 tcaactatgt atagcttcag gttttgccat tttaggatcg cttctttttg taaccatttt
     7981 aaaaatgatt acttttaagg ataatatttt tatacctctt atcggactga tgcttggaca
     8041 aattgtagca gcagcaacag tatttttagg aacacatttt caagtactac aatctgtcaa
     8101 cagttggctt caaggtaatt tttccatcat gactagccat cgctacgaaa ttctttactt
     8161 agcattgcca tgtttgtttt tagtatattt ttttgctcat cagtttacca ttgttgggct
     8221 tggagaaagc ttcgctaaga atttaggggt tgcttatgaa aagatgattt actttggttt
     8281 agttttggtt tcaattatga catccctagt cattattatc gtaggagctt tacctttctt
     8341 aggtttaatc gtacctaatc taattagcat aactaaagga gatcatatgt cctcaactat
     8401 cttagagaca tcgcttttag gggcttgtat cgtcatgata tgtgatcttt ttggtcgctt
     8461 agttattttt ccttatgaag tctcaatagg agttacgcta ggagtattag gtagtgcctt
     8521 tttcctgatt tcaattataa gaaatgaggg aaaatcatga ttaaggattt agagatacca
     8581 aaattactta ttttactgat actactaatt gctgctatta ttttgttttt aatttatggt
     8641 attccaactg acgcaaatga atttttgatt atctatatcc taaaaacacg ttatcaaaaa
     8701 ctgattgcat taattttggt tggtatctgc attggtagtt ctagtcttat ttttcaaact
     8761 ctaaccaata accgactttt aactccgtcc attattggtt tagatagctt atatatcctt
     8821 atccaaacag gattaatgta tttaatcgga gctcagcgtg tcataaaatt ttcatctttt
     8881 agtagcttcc tcctttcatt attattaatg gttggatttg cttacttatt atttacgata
     8941 ctttttcgaa ataaaaaaca gtctttgtat tttgttctat tagctggact catatttaat
     9001 acattatttt cttctatctc atcctttatt caagccatta tggaccccaa cgattttatg
     9061 attttacaaa atcagctctt tgctagtttc aatgctatta acaccaagat cctttggatt
     9121 tctttcatta tcattgtcgt tagctttgtc attaattggc cttttataaa ggaattggat
     9181 gtcttactat tgggtaaaga aaatgcgata tcactcggca tctcatatca aaagcttact
     9241 actagatttt ttttatggtt agcattgatg gtagctatag caacagcatt agttgggccg
     9301 attacttttt taggcttact tgtagcacat attacctatc acagttttca tacctttaga
     9361 catcaaatat tagttcctat agccattgtt atctgtatct ttacacttgt cttagggcag
     9421 catctcgttc aaaatctatt acacctcact gttcaactaa gtgttctcct caaccttatc
     9481 gggggaagtt attttatatt caccttaatc aaaggaagga aaaacttatg attcaaataa
     9541 acaatttaca taagttctat ggccaaaagg aaatattaaa agacattaat atttcgattc
     9601 ctaaaggaaa agtaacagct attttaggtc caaatggatc tggaaagagt acattgctga
     9661 gttgtatcag tcgacttgaa ccttatgata atggtgaaat tttccttgat aaagtaccat
     9721 tagcccacta cagttcaaat gacttagcca aaacattagc catattaaga caatctaatc
     9781 acttaacact caagataaag gtgagagact tgataggatt cggtagattt ccttatagta
     9841 aaggacggtt aagtcaaaaa gataaagctg tcatagaatc agttatatct tatatggatt
     9901 taaacgatat agctgacgag ttcattaata acctcagcgg aggtcaaatt caaagagctt
     9961 ttatcgcaat gacaatggca caagatactc aatatatttg cttagatgag ccactcaaca
    10021 atttagatat gaaatatgct gttcaaatga tggatcttat caaacgatat gcgtatgagt
    10081 ttaataaaac tatagtgatt attattcacg acatcaattt tgcaactcac tacgctgaca
    10141 atgttgtcgc tttaaaagaa ggacaagtgg tgacttgtgg gactgtcgaa gatgttatgc
    10201 aagaaaaaat cctttctcat ttatttgata tgcctatccg tatcgaaact gttgatggta
    10261 aaccaattat ccttttttgt acaacgtaaa taaagagatt tatgtaggat aacattttta
    10321 gtttttatct attttaggag attttattta atgacaaaaa aacttattat tgctatatta
    10381 gcactatgca ctatcttaac cacttctcaa gctgttttag ctaaagaaaa atcacaaact
    10441 gttaccataa aaaacaacta ttcggtctat attaaaaaag aaaaaagaga caagccggat
    10501 aataaaaagc aaatcagcga gacacttaaa gttcctttaa aacccaaaaa agtagttgtt
    10561 tttgatatgg gagctttgga tactatcaca gctttaggag ctgaaaaatc tgttattggt
    10621 atcccgaagg ctaaaaatgc tctaagttta ttgcccaata acgtcaaatc tgtttataaa
    10681 gctaagagat accaagacgt aggaagtctc ttcgaaccaa actttgaagc tattgctcgt
    10741 atgcaacctg atgtggtttt cctaggagca cgtatggctt ctgttgataa tattgaaaaa
    10801 ttaaaggagg ctgcacctaa agcagcatta gtatatgctg gagtcgactc aaaaaaagta
    10861 tttgacaaag gagttgctga gcgtgtcaca atgttaggga aaatcttcga ccaaaataaa
    10921 aaggcaaaaa cctttaataa agatatcgca caagctgttc ttaaattgca gaaaactatt
    10981 gagaaaaaag gtaaacctac agctctattt gtaatggcaa acagcggtga acttttaact
    11041 caatcacctt ctggtcgttt tggttggatt ttctctgtag gtggatttaa agcagtcaat
    11101 gaaaatgaaa aactaagttc acatggtact cccgtatctt atgaatacat cgctgaaaaa
    11161 aatcctaact atctctttgt tttagatcgt ggagcgacta ttggacaagg agcttcatca
    11221 aaagaacttt ttaataacga tgttattaaa gcaactgatg ctgtcaaaaa caaacgtgtt
    11281 catgaggtag atggaaaaga ttggtatatc aattcaggcg gaagccgagt aacactccgt
    11341 atgattaaag atgtacagaa ctttgttgat aatcgttaat tttttgtcaa aggctaggaa
    11401 aaatcctagt cttatttttt tgaaaaaata gctattatca atgcttcaga gtatagattg
    11461 atcttatcac tttattttgt ctattatttt cgaatatagt caatggaggt aaccttatga
    11521 atcattttga actatttaag cttaaaaaag ctggattaac aaatcttaat atcaacaata
    11581 ttatcaacta tctcaaaaag aatagtttaa cttctctatc tgttcgcaat atggccgtag
    11641 tatcaaaatg taaaaatcct actttcttta tagaaaatta taaacagcta gaccttaaaa
    11701 aacttcgaca agaattcaaa aaatttccag ttctatcgat tttggattct aactatcctt
    11761 tagagttaaa agaaatatat aatccaccag ttctactttt ttatcagggt aatattgaac
    11821 ttctatctaa acctaaatta gctgtagtgg gggcaagaca ggcatctcag ataggttgtc
    11881 agtctgttaa aaagattatc aaggagacta acaatcaatt tgttatcgta agtggtttag
    11941 cgcgtggcat tgatacagca gcacatgtta gtgctttaaa aaatggcggc agtagtatag
    12001 ctgttatcgg gagtggttta gatgtttatt atccaacgga gaataagaaa cttcaagaat
    12061 atatgtcata taatcatctc gtattatcag aatattttac cggagaacaa cccttgaaat
    12121 ttcatttccc cgaacgtaac cgtattattg cagggctgtg tcaaggtatt gtggttgccg
    12181 aagctaagat gagatctgga agtttaatta cctgtgaaag agcattagaa gagggacgag
    12241 aagtttttgc cattcccgga aatattatcg atggcaaatc agatggatgc caccatctta
    12301 tccaagaagg agctaaatgc attatttcag gaaaagatat cctttctgaa tatcagtagt
    12361 gttttgcttt ctactatagg agaagttttc tattgacagt ttaagaaaag tgagttatta
    12421 tgctataagt ttacgatata gaaagtgata cctatggcaa ctacgacaaa aacgtcgacc
    12481 aaaaaaacaa gtaaaaaaaa atcagctact gctaaaaaaa atctagttat tgtggagtct
    12541 cctgcaaaag caaagactat cgaaaaatac ttaggacgta actataaagt tgtagcctca
    12601 gtaggtcata tccgtgattt aaaaaagtca agtatgtcca ttgattttga gaataattac
    12661 gaaccacaat atattaatat tcgtggtaaa ggacctctca ttaatgattt aaaaaaagaa
    12721 gctaaaaagg ctaaaaaagt ttacctcgcg agtgacccgg accgtgaagg agaagctatt
    12781 tcctggcatt tagcgcatat tttagattta gacaaagaag acagaaaccg tgttgttttc
    12841 aatgaaatca caaaagatgc tgttaaaaat gcttttgtgg aacctcgtca gattaacatg
    12901 gatctcgttg atgctcagca agcacgacgt gttttagatc gcattgttgg ttattctatc
    12961 tcacctattt tatggaaaaa agtaaaaaaa gggctatcag ctggacgtgt acaatcagtt
    13021 gctcttaaac taatcattga tcgtgaaaat gagattaagg ctttccaacc ggaggagtat
    13081 tggactattg acggttcctt taaaaaagga acacgtaaat tcaatgccac tttctacggt
    13141 ttagacggaa agaaattcaa attatcgaat aatgaagacg tcaaaacagt tctaaaacgt
    13201 attaaaactg atgaattctt agttgaaaaa gttgaaaaaa aagagcgtcg tcgtaatgca
    13261 ccattaccgt atacaacttc ttcattgcaa caagatgcag ctaataaaat caattttcga
    13321 actcgtaaaa ccatgatgat tgcgcaacag ctgtacgaag gacttagctt aggcacagca
    13381 ggtcatcaag gtctgattac ctatatgcgt actgattcta cacgtattag tccgttagca
    13441 caaaatgaag caacagaatt tattactaac cgttttggtg caaattattc taaacatggg
    13501 aataaagtta aaaatgcttc tggagcacaa gacgctcacg aagctattcg cccatctagt
    13561 gttaatcata cacccgaaag tattgctaag tatttagaca aagatcaact aaaactttac
    13621 actcttatct ggaaccgttt tattgcaagc caaatgacag cagcagtctt tgacacaatg
    13681 aaagttaatt taacgcaaaa tggtgttact tttattgcta atggtagcca agtcaagttt
    13741 gatggttaca tggctgttta taacgatact gacaaaaata aaatgttacc agatatggag
    13801 gaaggagaaa gtgttaaaaa ggttaatacg aatcctgaac aacacttcac tcaacctcct
    13861 gcaaggtttt cagaagcaag tctcattaaa acacttgaag aaaacggtgt aggtcgtcct
    13921 tcaacttatg ccccaacgct tgagactatt caaaaacgtt attatgttaa gcttgcagct
    13981 aaacgcttcg aaccaactga acttggtgaa attgttaata gccttattgt agaatttttc
    14041 ccagatattg ttgatgttac cttcactgcc gaaatggaag ggaaactaga cgaagttgag
    14101 attggtaaag agcagtggca aaagattatc gatgaatttt ataaaccatt tgaaaaagaa
    14161 cttgcaaaag cagaaactga aatggagaaa attcaaataa aggatgaacc tgctggattt
    14221 gattgcgagc tatgtggatc accaatggta ataaaactag gacgttatgg aaagttttat
    14281 gcgtgtagca atttccctga atgtcataac acaaaagcta tcactaagga aataggtgtt
    14341 atttgtccta tctgtcaaaa aggacaagtt attgagcgaa aaacaaaacg caatcgtatc
    14401 ttttatggct gtgaccgcta cccagaatgt gagtttacat cttgggacaa acctattgga
    14461 cgaacttgcc ccaaatcaaa tgatttctta gtggagaaaa aagtacgcgg tggtggtaaa
    14521 caagttgttt gctcaaatga aaaatgcgac taccaagaag agaaaattaa ataaagtgga
    14581 atctgagtgt ttagctcaga tttttaaaaa aattattatt ttacataact taatttatgt
    14641 atttattttt atgggagaat taataaaaaa gtgctataat ataggtgtat tcatagtgtg
    14701 aggtgccgtt atgtcctata ttgaaatgaa gcattcctat aagcgttatc aaacaggtga
    14761 aaccgaaatt gttgctaaca atgatattag tttcagtatt gaaagaggtg agttggtggt
    14821 tattcttggt gcatccggcg ctggcaaatc tactgttctc aatatactag gagggatgga
    14881 tagtaatagt gaaggtgaag ttttaattga tggtaagaat attgctaatt ataccatcag
    14941 ggaattgact cgttaccgta gatatgatgt cgggtttgtt tttcaattct ataacttagt
    15001 ccctaatcta acagcactag agaatgtaga attggcttca gaaattgttc caaaggcatt
    15061 agatgcccaa caggcattag aaaacgtagg tttgggacac cgtatcaatc attttccagc
    15121 acagctttcc ggtggcgaac agcaacgcgt tgcaatagca cgagctattg ccaaaaaacc
    15181 taaactgtta ctttgtgatg aacctactgg tgctttagat taccaaactg gaaaacaagt
    15241 actagccatc ctgcaaaaga tggcgcaatc aaaagaaaca actgtgatca ttgttactca
    15301 taatactgcc ttagcgccaa ttgcaaatcg cgtgatccat atgcatgatt caaaaatcag
    15361 tgatattgta ataaacgaga atccaagtga tattcagaac atagaatatt agaggttata
    15421 cagatgggaa agacattttg gaaagatatc tatcgatcaa ttacgacttc aaaaggtaga
    15481 ttttcatcaa tcctcttact tatgatgtta ggctcatttg cttttatagg actgaaggtc
    15541 tcagcaccta atatgcaaag aacagcacaa aattaccttg ctcatcatca tgttatggat
    15601 attaccgttt tcaattcttg gggacttgac aaacatgatc aaactgtttt agaaagctta
    15661 aaaggatcac aagttgaatt ttcttacttt gtagatacaa cccctcaaca gaatagtaaa
    15721 tcttatcgat tgtattccaa taccaaaacg atatcaacct ttgatcttgt taagggacgt
    15781 ctccctttaa ataaatcaga aattgctctc tcatttcagg aacgaaaaaa atatgcaata
    15841 ggtgataaaa ttaattttaa acaagataaa aacaaactgt tttcaaatac aggtcctcta
    15901 actattgtag gttttgttaa ttctaccgag atttggtcta agacaaacct tggtagttct
    15961 caaacagggg acggcgactt agatagttat ggcgtattag ataagacagc atttcactct
    16021 ccagtatata caatggcaag ggtaactttt aaagatctta ggttgattaa ccctttctca
    16081 ataagttaca aagaaaaagt agcgaaatac caagaaaagg tatcgcgaaa attaaatatt
    16141 cacaataaaa taagatatac caaaacaaaa aaagagagcc tacgtaaaat cgatgaggag
    16201 gaaaaaagcc tactaaaagc tcaaaaacaa atcaatcggt tagataatga tagcctagcg
    16261 atgccactat ctcaaagaca agctattcaa atgaaaatta aacaagaccg actatctctt
    16321 ttgaaacgaa caaaagagct tctaaaattg agacataaca cccaaataat ggaatcgcct
    16381 caaattattg tctacaaccg tactaccttt cctggaggac aaggatataa cacttttgac
    16441 tccagtacaa atagcacttc taaaatcagt aatcttttcc ccattatttt atatttagtc
    16501 gcagcattag taaccttaac aacaatgact aggttcgtcg aggaagaaag aaccaacgca
    16561 ggcatattaa aagcactagg ctatagtgac cgccaagtta tctttaaatt tattatttat
    16621 ggttttatag caggaacatt agggactact ttgggtatca ttggagggca ttatctttta
    16681 cctcgtatta tttctgatat catttccaaa gacctgacta ttccaaacac acagtatcat
    16741 cttttcttaa attacagttt actagccttt gttttttcat tattaagcat tgttcttcca
    16801 gtttttgtta tcactcgacg tgagttgaaa gaaaaagcag catttttatt attaccaaaa
    16861 ccaccagcta aaggatctaa aatagcttta gagtatatca attggatttg gaaaaaactg
    16921 tccttcacac aaaaagtaac tgctagaaat atttttcgtt acaaacaaag gatgattatg
    16981 accatatttg gagttgctgg atcagtagcg ctcttatttt caggtctagg aatacaatcc
    17041 tccttaaagc aaaccgtcaa tgaacatttc ggtcgtatta tgccctatga tatattgcta
    17101 acatataaca caaatgcttc tcccccaaaa atacttgaac tactttcaaa agattcaaaa
    17161 atagacaaat accaaccaat acaccttgaa aatcttgacg aatccattcc tggacaaatt
    17221 aataaacagt caatttccct attcataaca gacaaaaaac aattactacc ttttatttat
    17281 ttacaagaag caacaactaa taagtcattg cacttgaata ataaagggat tattatttct
    17341 aaaaaattag ctcaatttta tcatgttaat accggtgatt ttatccattt atctcattca
    17401 caaacacttc cttctagaaa attaaaaata acaggagttg tcaatgcgaa tgttggtcac
    17461 tatattttta tgacaaaaca atactatcga actattttta agaaagaagc taaagataac
    17521 gcttttttgg ttaagttaac caaacataaa atcgcaaata acttagcaga aaaactttta
    17581 gaaattaatg gagttgagtc tcttacgcaa aatgctcttc agctggctag tgtagaagcc
    17641 gttgtgcgtt ctttagatgg ctctatgact attttagttg tcgtatcttt attgttagcc
    17701 attgtaattc tctataatct cactaatatt aatttagcag aacgtaagcg ggagctatca
    17761 actataaagg tattaggttt ttataatgaa gaagtaactt tatacattta ccgcgaaacc
    17821 attattttat caaccatcgg tgtgattcta ggcaccatta gcggtactta tttacaccgt
    17881 caaatgatgt tgctaattgg ttcagatcaa atactttttg gtgaaaaagt a
//
LOCUS       DATRNK010000045        17264 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.045, whole genome shotgun sequence.
ACCESSION   DATRNK010000045 DATRNK010000000
VERSION     DATRNK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17264
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.045"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..386
                     /locus_tag="VBC52_001436"
     CDS             <1..386
                     /locus_tag="VBC52_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6883017.1"
                     /translation="GDTLDEVLEKYSILVNQKKININKELLDYNIYIGKTALNIVFSN
                     LISNAVKYTNRNGIINIKIANDWLLIENSYDKNKILDASFDLKLDNSNGLGLNIVKNI
                     LNKYNIKYEILHGENYFIFKIKIIS"
     gene            complement(438..1085)
                     /locus_tag="VBC52_001437"
                     /pseudo
     CDS             complement(438..1085)
                     /locus_tag="VBC52_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1263..1442
                     /locus_tag="VBC52_001438"
     CDS             1263..1442
                     /locus_tag="VBC52_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6883018.1"
                     /translation="MSQEKLKSKLDQAKGGAKEGFGKITGDKELEAKGFIEKTIAKGK
                     ELADDAKDAVKEKLK"
     gene            1484..1672
                     /locus_tag="VBC52_001439"
     CDS             1484..1672
                     /locus_tag="VBC52_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO6883019.1"
                     /translation="MSIRTDIDEFKEAIDKGYISGNTVAIVRKNGKIFDYVLLHEEVR
                     EEEVVTVERVLDVLRKLS"
     gene            2097..3302
                     /locus_tag="VBC52_001440"
     CDS             2097..3302
                     /locus_tag="VBC52_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEO6883020.1"
                     /translation="MSQKSNYFKGQIDYAVVIPVFFLLMIGLASIYVATMNDYPSNIY
                     IAMFQQVSWIIMGCIIAFVVMLFSTEFLWKATPYLYALGLTLMVLPLIFYSPQLFAAT
                     GAKNWVTIGSVTLFQPSEFMKISYILMLSRITVSFHQKNRKTFQDDWKLLGLFGLVTL
                     PVMILLMLQKDLGTALVFLAILSGLILLSGISWWIILPILSTIVLFIASFLMIFISPN
                     GKEWFYNLGMDTYQINRLSAWIDPFSFADSIAYQQTQGMVSIGSGGVTGKGFNILELS
                     VPVRESDMIFTVIAENFGFIGSAIVLGLYLIIIYRMLRITIESNNQFYTFISTGFIMM
                     IVFHVFENIGAAVGILPLTGIPLPFISQGGSSLLSNLIGIGLVLSMSYQNTVRQAKAN
                     GSHLKYRSI"
     gene            3421..3981
                     /locus_tag="VBC52_001441"
     CDS             3421..3981
                     /locus_tag="VBC52_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEO6883021.1"
                     /translation="MNYHDYIWDLGGTLLDNYESSTRAFVETLKEFGYQADHDSVYQK
                     LKESTADAIAYFIPEEADFLKEYKANEAKVLETPILFQGAKELLAKIQRQGSRNFLVS
                     HRDNQVIVILEKTEIIDYFTEVVTADNGFSRKPSPESMLYLKEKYQIDNCLVIGDRDI
                     DKQAGESAGFDTLLVDGSKSLMEIIE"
     gene            3982..5934
                     /gene="gyrB"
                     /locus_tag="VBC52_001442"
     CDS             3982..5934
                     /gene="gyrB"
                     /locus_tag="VBC52_001442"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEO6883022.1"
                     /translation="MTEETKNMEQRAQEYDASQIQVLEGLEAVRMRPGMYIGSTSKEG
                     LHHLVWEIVDNSIDEALAGFAGHIKVYIEPDNSITVVDDGRGIPVDIQEKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKNGKVHYQEYQRGVVV
                     NDLEIIGDTDLSGTTVHFTPDPEIFTETTVFDFDKLAKRIQELAFLNRGLRISISDKR
                     EGQEVEKEYHYEGGIGSYVEFINENKEVIFENPIYTDGELDGISVEVAMQYTTGYQET
                     VMSFANNIHTHEGGTHEQGFRTALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFNRFLLENPQVAKKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNNAEMNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKVRYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKAYIQPGVNQEEE
                     LRQALDTYSSGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVSVDDAAEADKIF
                     DMLMGDRVEPRREFIEANAVYSNLDI"
     gene            6028..7752
                     /gene="ezrA"
                     /locus_tag="VBC52_001443"
     CDS             6028..7752
                     /gene="ezrA"
                     /locus_tag="VBC52_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEO6883023.1"
                     /translation="MSSGIILLLVAIVLLVIIAYIVGVVIRKRNDTLIANLETRKQEL
                     VDLPVQEEIEQVKLLHLIGQSQSTFREWNQKWTDLSTNSFKDIDFHLVEAENLNDSFN
                     FVRAKHEIDNVDSQLTIIEEDIVSIREALEVLKEQEEKNSARVTHALDLYETLQKSIS
                     EKEDNYGTTMPEIEKQLKNIEAEFSHFVTLNSTGDPIEASEVLNKAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKDIEQRFQEVREAIRSNSDGLV
                     SLDLDRARDENEHIQEKIDKLYDIFEREIAAYKVAHKDSKIIPQFLAHAKSNNEQLGH
                     EIKRLSAKYILNENESLSLRSFTNDLEEIETKVLPSVENFGQEASPYTHLQILFERTL
                     KTLTTVEENQMEVFEAVKTIESVETRARQNMDKYVNKLHMIKRFMEKRNLPGIPQDFL
                     STFFTTSSQIEALINELSRGRIDIEAVSRLNDVTTNAIANLEQATYLVVQDATLTEQL
                     LQYSNRYRSFEQNVQKSFEQALYLFEVEHNYKASFDEISYALETVEPGVTDRFVTSYE
                     KTQERIRF"
     gene            7846..8487
                     /gene="serB"
                     /locus_tag="VBC52_001444"
     CDS             7846..8487
                     /gene="serB"
                     /locus_tag="VBC52_001444"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEO6883024.1"
                     /translation="MIKGLLVMDVDSTLIMEEAIDLLAIEAGVGKQVAALTDAAMRGE
                     LDFEEALKKRVALLKGLPVTILTDILSSIHFTPGAYELIKECHKRQMKVGLVSGGFHE
                     TIDILAKQLQVDYVKANRLGVKGGFLTGEVEGEIVTKEVKKIKLKEWASENHLDLSQT
                     IAMGDGANDLPMIKSAGVGIAFCAKPIVREEAAYQINEKNLLKVFDIIDANEN"
     gene            complement(8508..8993)
                     /locus_tag="VBC52_001445"
     CDS             complement(8508..8993)
                     /locus_tag="VBC52_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO6883025.1"
                     /translation="MEIWDAYDQYGFITGLTLNRDQNIPQGLFHLVVDVILFHEDGDV
                     LMMKRHPKKKAFPAYFEATAGGSALKGENAKQAILRELKEETGIVPQCLTFLNREWFS
                     ERSYFVDHFIAKYNGAKDIITLQEGETVDYIWLKPEYIDLFLSKNKLIPSQIKLLKSL
                     I"
     gene            complement(9006..9461)
                     /locus_tag="VBC52_001446"
     CDS             complement(9006..9461)
                     /locus_tag="VBC52_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEO6883026.1"
                     /translation="MTELDNKILQKASGENRLDPDQQRYYLGTYKERVVLHLSLKEAQ
                     EEKFQLAITSALNNLILEYPNLSLKLSPNLPTDVQMFYMKLANRLSLAATIVNEANAN
                     SPFGIIIHSDKTENVEWNDFETQFPDLFNSPKKEESPKKSLWQHFFSQK"
     gene            9659..10966
                     /gene="eno"
                     /locus_tag="VBC52_001447"
     CDS             9659..10966
                     /gene="eno"
                     /locus_tag="VBC52_001447"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEO6883027.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNVIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEALRWGAEVFHALKKILKERGLETAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDAERKVYDYGKFEGEGGAVRTAAEQI
                     DYLEELVNKYPIITIEDGMDENDWDGWKALTERLGGRVQLVGDDFFVTNTDYLARGIK
                     EEAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            complement(11074..12138)
                     /locus_tag="VBC52_001448"
     CDS             complement(11074..12138)
                     /locus_tag="VBC52_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6883028.1"
                     /translation="MKRKQFIKLGIATLLTVISLYTPINLATNHTTENIVTAQEYKTK
                     ENGTLPFKHKRQLVLGELDDKGRATFAHIQLKVKDEPKKKRVKRLKTTPVGWHNFKFY
                     YNDGTQKAWLMSRGRLICHQFSGLNNERKNLVLMTNWLNTGNYNSTNSSNPESMLFYE
                     KQLKTWLSTHKNYYLDYKVTPIYQNNELIPRKIELKYVGIDKTGKLLPIFIGNKSTQD
                     QFGISTVTLENTSPNATIDYLSGKAQNTVLSAKEQRKLIAKHEEEKRLAEKKAEEEKA
                     AAETQKKLEEEQARLAAEAQRKQEEEQARLAAETQKKQETLVQEQTSQGYKRDYRGRW
                     HRPNGQYASKAEIAAAGLQW"
     gene            12367..13650
                     /gene="aroA"
                     /locus_tag="VBC52_001449"
     CDS             12367..13650
                     /gene="aroA"
                     /locus_tag="VBC52_001449"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEO6883029.1"
                     /translation="MKLLTNANTLKGTIRVPGDKSISHRAIIFGSISQGVTRIVDVLR
                     GEDVLSTIEAFKQMGVLIEDDGEIITIYGKGFAGLTQPNNLLDMGNSGTSMRLIAGVL
                     AGQEFEVTMVGDNSLSKRPMDRIALPLSKMGARISGVTNRDLPPLKLQGTKKLKPIFY
                     HLPVASAQVKSALIFAALQTKGESLIVEKEQTRNHTEDMIRQFGGHLDIKDKEIRLNG
                     GQSLVGQDIRVPGDISSAAFWIVAGLIIPNSHIILENVGINETRTGILDVVSKMGGKI
                     KLSSVDNQVKSATLAVDYSHLQATHISGAMIPRLIDELPIIALLATQAQGTTVIADAQ
                     ELKVKETDRIQVVVESLKQMGADITATADGMIIRGNTPLHAASLDCHGDHRIGMMIAI
                     AALLVKEGEVDLSGEEAINTSYPNFLEHLEGLVNA"
     gene            13643..14155
                     /locus_tag="VBC52_001450"
     CDS             13643..14155
                     /locus_tag="VBC52_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEO6883030.1"
                     /translation="MPKVLLGFMGVGKTSVANCLENEVIDMDSLIEKHIGMSISRFFT
                     EEGEASFRALESQFLNELLKKKNEGLVIASGGGIVLLEENRRLLTLNRHNNILLTGSF
                     EVLYHRIKKDEKNRRPLFLNHSKEEFYDIYQKRMLLYSGLSDMIIDTDYLTPQKIATV
                     IGESLVNLSN"
     gene            14212..15585
                     /locus_tag="VBC52_001451"
     CDS             14212..15585
                     /locus_tag="VBC52_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6883031.1"
                     /translation="MSRNNYGQLNHHEELRYNYLLKNIHYLNEREKMEFQYLHYKKTA
                     VRPQRRTESPPTNSYYEEPYSDSYYQDDDFYSEPQLTSQGLPIYQEERAPKKKKQRAR
                     KEKQRVKVMAPFPPKAITPPRKKKKFKGFLKFIGIILLIVLSGMVFMFVKGMRDVNNG
                     KSHYSPAIIEDFKGKDAVDGTNILILGSDKRVSERSTDARTDTIMVANVGNKDNKVKM
                     VSFMRDLLVNIPNYSTEGYYDMKLNASFNLGEQDNHKGAEYVRQTLKNHFDIDIKYYV
                     MVDFETFADAIDTLFPNGVKINAKFGLVGGQSADSVKVPDDLRMKNGVVPSQKIKVGI
                     QYMDGRTLLNYARFRKDDDGDFGRTQRQQQVMRAIVSQIKDPRRLFTGSAAIGKAYAL
                     TSSNLSYSFVLTDGIPILSDAKNGIKQMTIPREGDWVDDYDQYGGQGLTIDFAKYKKI
                     LKKMGLR"
     gene            15686..17041
                     /gene="rlmD"
                     /locus_tag="VBC52_001452"
     CDS             15686..17041
                     /gene="rlmD"
                     /locus_tag="VBC52_001452"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO6883032.1"
                     /translation="MLHKNDIIETEISDISHEGMGIAKVDGFVFFVENALPGEIIKMR
                     ILKLRKRIGYGKVEEYLTTSPHRNEGLDYTYLRTGIADLGHLTYEQQLLFKQKQVADN
                     LYKIAHISDVLVEPTLGMTIPLAYRNKAQVPVRRVDGQLETGFFRKNSHTLVSIEDYL
                     IQEKEIDALINFTRDLLRKFDVKPYDEEQQSGLIRNLVVRRGHYTGQLMLVLVTTRPK
                     IFRIDQMIEKLVSAFPSVVSIMQNINDRNSNVIFGKEFRTLYGSDTIEDQMLGNTYAI
                     SAQSFYQVNTEMAEKLYQKAIDFSDLNSEDIVIDAYSGIGTIGLSVAKQVKHVYGVEV
                     VEKAVSDAKENATRNGITNSTYVADSAENAMAKWLKEGIKPTVIMVDPPRKGLTESFV
                     YSAAQTKADKITYISCNSATMARDIKLFEELGYHLVKIQPVDLFPMTHHVECVALLVK
                     A"
BASE COUNT         5704 a         2591 c         3390 g         5579 t
ORIGIN      
        1 ttggtgatac attagatgaa gtattagaaa aatactctat cttagtaaat caaaaaaaaa
       61 taaatattaa taaggaatta ttagactata atatttatat aggaaaaacg gcactaaata
      121 ttgtattttc taatttgatt agcaatgcag taaaatatac taatcgaaat ggaataatta
      181 atatcaaaat agctaatgat tggttactta tagaaaactc atatgataaa aataaaattt
      241 tagatgctag ttttgatcta aaattggata atagtaatgg attaggatta aatatagtaa
      301 agaatatact taataagtat aatataaagt atgaaatatt acatggtgaa aattatttta
      361 tatttaaaat aaaaataatc tcttgagtaa taatcatact attggactaa tgtcaaataa
      421 gtagacagaa aaccgtgtta ttttattgcg ttaaaataat tttcttcttt ctgattaggg
      481 gttagtccta gattagccgt atgtgggatg taattgttat aaaaattctc aatgtattca
      541 aagcagtcta attgaacctg tttgatattt tgataatttt tttggttgat ttgtctatgc
      601 tttaaatact tgaaaaatgc ttcagttacg gcattatcat aaggatatct aggtttagaa
      661 aaagaatgca tgatattgtt gtcatcaatt atttttctaa attccctggc cttaaattgt
      721 gacccttggt cggaatgaaa gagaagtgtt ccttcaatct ttcttttatt aagagctaat
      781 tctagagtgt cacatgctaa ctttgcatcc atacgatgac tcaatttcca agcaatatat
      841 tttctagaat agagatcaat tattgcgcag agatagacat atttcttata tccaatagaa
      901 atataggtga agtcagtaga ccatacttga ttaggtttat caggattaaa tttctggttg
      961 agtaagtttt gaggatacgt tttttgagtc tttttaagag ctgtttttgt cttaacggta
     1021 gccattttag ggagcgccat attcttcatc agacggtaaa ttcttccttc agagattttt
     1081 gtgtctttta aaacaggtta taacacacaa agtgttgtct atttttatag tatacatcca
     1141 tactcgcata accatgagat aatcactaaa acaagagtcg cctgacacta gcgactcttg
     1201 tttttatttg ctaaagaaag atatagatgt tatgattaat aaaaataata aggaggtgca
     1261 gtatgtcaca agaaaaacta aaatcaaaat tagatcaagc aaaaggtggt gctaaagaag
     1321 gctttggcaa aataaccggt gataaagagt tagaagcaaa aggatttatc gaaaaaacaa
     1381 ttgctaaagg caaagaacta gcagatgatg ctaaagatgc tgtcaaagaa aaactgaagt
     1441 aacaaataac cgctcattta acgagcggtt atttgttatc tttttgtcta tcagaacaga
     1501 tatagatgag tttaaagaag cgattgataa aggctatatt tcagggaaca cagtagcgat
     1561 agtgcgtaaa aacggaaaga tatttgatta tgtgttacta cacgaagaag tgagagaaga
     1621 agaggttgtt acagttgaga gagtgcttga tgtactgagg aagttatcat aataacggac
     1681 caaaattcgc cccaaaaagt attttaaaat agaataaaat aaaaaaatat gctttttatt
     1741 atggatgtat cgtgtatttt aacctttaat atatcctatt ttaatggtag agaagttggc
     1801 aggggacata ttaaaacctt ttttgagatg ttaggaagga ttttattctt tagactagtg
     1861 ttgagaaaat aacaaaactt aaataagatt atttaagttt tgtttaaggt gaactttgta
     1921 tactttaaat atcctaaata aacttttctt ttcatgttct ttgttttact gatgaagaac
     1981 aatctcctaa tatttgcaac tcataatgat ttgagttgca ttttttattg caaaaaatat
     2041 aagtcagatt ctgaaaaaat gttataataa ttaccatgaa taaaaaagga agtcctatgt
     2101 cacaaaaatc aaactatttt aaagggcaaa ttgattatgc tgttgtgata ccagtatttt
     2161 ttttattaat gattggttta gcctcaattt atgttgcaac gatgaatgat tatccttcta
     2221 atatctatat cgcgatgttt cagcaggtgt cttggattat tatggggtgt attattgcat
     2281 ttgtcgttat gcttttcagt actgaatttt tatggaaagc cacgccctat ctgtatgctt
     2341 taggactaac cctaatggtt cttcccttaa ttttttatag tccacagctt tttgcggcaa
     2401 caggtgctaa aaattgggtg accattggct cggtaacttt gtttcaaccg tcagagttta
     2461 tgaagatttc ctatatttta atgttatctc gaataactgt tagttttcat cagaaaaatc
     2521 gcaaaacttt tcaagatgat tggaagttgc taggattgtt tggtttggtt acattgccag
     2581 tgatgattct tctaatgtta caaaaagatt taggaacagc attagttttc cttgccattt
     2641 tgtcaggttt aatcctttta tcgggcatat cttggtggat aatcttgcca attttatcta
     2701 ctatcgttct ttttatagct agctttttga tgatttttat ctcaccaaac ggcaaggaat
     2761 ggttttataa tttggggatg gatacatatc aaatcaaccg tttatctgct tggattgatc
     2821 catttagttt tgcagactca attgcttatc aacaaacaca gggtatggta tcaattggaa
     2881 gtggcggggt aacaggtaaa ggttttaata ttttagagct ctcggttcct gttcgtgaaa
     2941 gtgacatgat ttttactgtc attgctgaga attttggctt cattggttcg gcgattgttt
     3001 tagggttata tctcattatc atttaccgta tgttaaggat caccattgaa tctaataatc
     3061 aattttatac ttttatatca actggtttta tcatgatgat tgttttccat gtttttgaaa
     3121 atattggtgc agcagttggt atattacctc taacaggaat cccacttccg tttatttcgc
     3181 aaggtggaag ttcattacta agtaatctta ttggaattgg attggtctta tccatgtctt
     3241 atcaaaatac agttcgtcag gccaaggcta atggatcaca tttgaagtat cgtagtatat
     3301 aacaaaaaaa ggttggaaag ataatccagc cttttaataa tcgaaatatt ttagaggagt
     3361 gagttaatta agtaaggtgg tagctataaa aaatcgtaat aatatgatat aataaagggt
     3421 atgaattatc atgattatat ttgggatttg ggtggtacac tacttgataa ttacgaaagt
     3481 tccacacgtg cttttgtaga gactttgaaa gaatttggat atcaagcaga ccacgatagt
     3541 gtgtatcaaa aattaaaaga gtcaacagca gatgcaattg catattttat ccctgaagaa
     3601 gctgattttt taaaagagta taaggctaat gaagctaagg ttttggaaac accaattcta
     3661 ttccaagggg caaaagaact tttagcgaaa attcaaagac aaggtagtcg taattttttg
     3721 gtgtctcatc gtgataatca agttatagtt attctggaaa aaacagaaat tattgactat
     3781 tttacagaag tagtgactgc tgataacggc ttttcaagga aacctagtcc cgagagtatg
     3841 ctttatttga aagaaaaata ccaaattgat aattgtttag taataggcga tcgtgatata
     3901 gataagcaag caggagaatc ggctggtttt gataccttat tagtcgatgg gtcaaaatca
     3961 ttgatggaga taatagagta aatgacagaa gaaacaaaga atatggaaca acgtgctcaa
     4021 gaatatgacg caagtcagat tcaagtatta gaaggtttag aagctgttcg catgcgtcca
     4081 ggcatgtata tcggttcgac ttcgaaagaa gggcttcatc accttgtatg ggaaattgtt
     4141 gataactcaa ttgatgaagc cttagctgga tttgcaggac atattaaagt ttacattgaa
     4201 ccagataact ctattacagt tgttgatgat ggtcgcggta ttccggtaga tatccaagaa
     4261 aaaactggaa gaccagctgt agagacagtt tttacagttc tccatgccgg tggtaaattt
     4321 ggtggtggcg gatacaaggt ttctggtggc ttacatggtg tagggtcatc tgttgttaat
     4381 gcattgtcaa cacaattaga tgtcaaggtt tataaaaatg gcaaggttca ttatcaagaa
     4441 taccaacgcg gtgttgttgt taatgatctt gaaattatcg gtgatactga cttatcagga
     4501 acaacagttc actttacgcc tgacccagaa attttcactg aaaccactgt atttgatttt
     4561 gataaattag ccaaacgtat tcaggaatta gcttttttaa atcgtggact aagaatctcc
     4621 atttcagaca agcgagaagg tcaagaagtt gaaaaagaat atcactatga aggtggtata
     4681 ggttcatacg ttgagtttat caatgagaac aaagaagtga tttttgaaaa tcctatttat
     4741 actgacggtg aattagatgg tatttcagtt gaagtagcaa tgcagtatac tactggatat
     4801 caagagactg ttatgagttt tgcaaataac atccacactc atgaaggagg aacgcacgaa
     4861 caaggatttc gtactgcctt gacacgtgtg attaatgatt acgctaaaaa gaataagatt
     4921 ctcaaagaaa atgaagataa tttaactgga gaagatgttc gtgaaggttt aacagcagtt
     4981 atatctgtta aacatccaaa tccacaattt gaaggacaaa ctaaaacaaa acttggaaat
     5041 tctgaagttg ttaaaattac aaatcgtctc tttagcgaag ctttcaatcg attcctatta
     5101 gaaaatccac aagtagctaa gaagattgtt gaaaaaggaa ttttggcatc taaagcccgt
     5161 attgcagcaa aacgggcgcg tgaagtaaca cgtaaaaagt caggcttaga gatttcaaac
     5221 ttacctggga aactggctga ttgctcatct aataatgctg agatgaatga actttttata
     5281 gtggagggag attctgcggg agggtctgca aaatcaggac gtaatcgtga gtttcaagca
     5341 attttaccga ttaggggtaa gattctaaac gttgaaaaag ctacaatgga taaaatttta
     5401 gcaaacgaag aaatccgaag tctcttcaca gctatgggaa ctggatttgg tgcagatttt
     5461 gatgtatcaa aagtcagata tcaaaaatta gtcattatga ctgatgccga tgttgatgga
     5521 gctcacatca gaactttact tttaactctg atttatcgtt ttatgcgacc tgttttggaa
     5581 gcaggatatg tttatatcgc tcaaccacca atctatggtg tcaaagtagg aagtgagatt
     5641 aaagcttata ttcagccagg ggtcaatcaa gaagaggaat tacgtcaagc tctagatacc
     5701 tatagttcag gacgttcaaa acctactgta caacgttaca aaggtctagg tgagatggat
     5761 gaccatcaac tttgggaaac aacaatggat cctgaaaacc gcttgatggc acgtgtctca
     5821 gttgatgatg cagcagaagc agataagatt ttcgatatgc taatgggaga tcgagtagaa
     5881 ccacgacgtg aattcattga agctaatgct gtttatagta acttagatat ctagtccaaa
     5941 gatagctttg aaaaacagtt caagtatgct ataatcttaa cgttatagta gatttttaga
     6001 aaattagacc attataagga gagttgaatg tcgagcggaa tcatattgct gcttgtggct
     6061 atagtcctgc ttgtgattat tgcctacata gtcggcgtcg taataagaaa acgtaatgat
     6121 acattaattg caaacttaga aactagaaaa caagagttag ttgaccttcc tgtgcaagag
     6181 gagattgagc aagtcaaatt gcttcatttg attggccaaa gtcaatcaac ttttagagaa
     6241 tggaaccaaa aatggactga tttatcaact aattcattta aagatattga ttttcattta
     6301 gttgaagctg aaaatcttaa tgattcattt aattttgtgc gtgctaagca cgagattgat
     6361 aatgttgata gtcagctaac tattatagaa gaagatattg tttcgattcg tgaagcttta
     6421 gaagttttaa aagagcaaga agagaaaaat agtgcccgtg taacacatgc tttggaccta
     6481 tacgaaacac ttcaaaaatc aatttctgaa aaagaagata attacgggac aaccatgcca
     6541 gaaattgaaa agcagttgaa aaatatcgag gctgaatttt cacattttgt gaccttaaac
     6601 tcaactggtg acccgattga agcttctgaa gttttgaata aagcagagga gcatacgatt
     6661 gctttagggc aaattacaga acaaattcca gctattgttg ctaaattaga agacgatttt
     6721 ccagatcagt tagatgattt ggagacagga tatcgtcgtc ttttagagga aaactaccat
     6781 ttccctgaaa aggatattga acaacgtttt caagaagttc gtgaggctat tcgttcgaat
     6841 tcagatggtt tagttagttt ggatctagat cgtgctcgcg atgaaaatga acatattcaa
     6901 gaaaaaattg ataaacttta tgatattttt gaacgtgaga ttgctgctta taaagtagct
     6961 cataaggata gtaaaattat tcctcaattc cttgctcatg caaaatctaa caatgaacaa
     7021 ctggggcatg aaatcaaacg attatctgct aaatatatct taaatgaaaa tgaaagctta
     7081 agtttacgtt ccttcacaaa tgatttggaa gaaattgaaa caaaggtttt accttctgta
     7141 gaaaattttg gtcaagaagc ttcaccttat actcatctac aaattttatt tgagcgtacg
     7201 ttgaaaacat tgactactgt tgaagaaaat caaatggaag tttttgaagc cgttaaaact
     7261 attgaaagcg ttgagacacg agcacgccaa aatatggata agtatgttaa caagctccat
     7321 atgattaaac gtttcatgga aaaacgcaat cttccaggaa tcccgcaaga cttcttaagc
     7381 actttcttta caacaagttc tcaaattgag gcacttatta atgaactcag tcgcggtcgt
     7441 attgatattg aggctgtttc acgtttaaat gatgtgacta caaatgctat cgctaattta
     7501 gagcaagcta catatctagt tgttcaagat gcgactctca cagagcagtt actacagtat
     7561 tcaaaccgtt atcgtagttt tgaacaaaat gtgcaaaaga gctttgaaca agctttgtac
     7621 ttatttgaag ttgaacataa ctataaagca tctttcgatg aaatttcata cgcacttgag
     7681 actgtggaac caggtgtaac tgaccgtttt gtgacgtctt atgaaaagac acaagaacgt
     7741 attcgtttct aaaaaagaag agttagtact cttctttttt taattgctat ctatgaaagg
     7801 tgctaatttt aaggtacaat agtaaaaaag ttttggaggt tttaaatgat taaaggtttg
     7861 ttagtcatgg acgttgattc aaccttaata atggaagaag ctattgattt attagctatt
     7921 gaagcagggg tggggaaaca ggttgctgca ctgactgacg ctgcaatgcg tggcgagtta
     7981 gattttgaag aagctttgaa gaaaagagta gcgctactta aaggtttgcc tgttactatt
     8041 ttgacagata tattatcctc aatacatttt actccagggg cttatgaatt gattaaagag
     8101 tgtcacaagc gtcagatgaa agttggtctt gtatcaggag gttttcacga gacgattgat
     8161 atcttagcta agcaattaca agttgattat gtcaaagcga atcgtcttgg tgtcaaaggt
     8221 ggttttctca ctggcgaagt tgagggagag attgtcacta aagaagtgaa aaaaataaag
     8281 ttaaaagagt gggcttctga aaatcactta gacctttcac aaaccatcgc aatgggagat
     8341 ggtgccaatg atttaccaat gatcaaatcc gctggggtag gaattgcttt ttgtgcaaaa
     8401 ccaattgtca gagaagaagc tgcgtatcag attaatgaaa aaaatttgct taaggtattt
     8461 gatattattg atgcaaatga aaactaggcc tttattaagc ctagttttta gattaaggat
     8521 tttaagagtt ttatttgaga gggtattaat ttgtttttag ataaaaataa atcaatatat
     8581 tctggtttta accagatata gtcaacggtc tctccttctt gcaatgtaat tatatctttt
     8641 gcaccattat attttgcgat aaaatggtct acaaagtaag aacgctcaga gaaccactct
     8701 ctattaagaa aggttagaca ttgaggaacg ataccagttt cttctttgag ttcccttaga
     8761 atagcttgct tagcattttc tcctttaagg gcagatccgc cagcggtggc ttcgaaataa
     8821 gcaggaaaag ctttcttttt agggtgccgt ttcatcatca atacgtcacc atcctcatga
     8881 aaaaggataa catcaacaac taagtggaat aagccttgtg ggatattttg atccctatta
     8941 agtgtaagac ctgtaatgaa cccatattgg tcataagcat cccaaatttc cattagacct
     9001 ccttgttatt tttgactaaa gaaatgttgc catagacttt ttttaggaga ctcctctttt
     9061 tttggtgaat tgaataaatc agggaattgt gtctcaaaat cgttccattc aacgttttca
     9121 gtcttatcac tatgtataat aataccaaat ggagaattgg catttgcttc attaacaatc
     9181 gtagcagcta gtgatagacg gttagctaac ttcatgtaga acatttgaac atcagtaggg
     9241 agatttggtg ataattttaa actaagatta ggatattcaa gtatcagatt attaagagca
     9301 gatgttatag caagttgaaa cttttcttct tgtgcctctt ttaaacttaa gtggagcaca
     9361 actctttctt tataagtacc aaggtagtaa cgttgttgat caggatcaag acgattttca
     9421 ccacttgctt tttgtaggat tttattgtca agttcagtca taatttctcc ttgaatttgc
     9481 taattttatt ataacctaaa agggttgcat tagcacaggt aaacttgaaa agaattagtg
     9541 atttttttat caaaaatcac gtaataaagc ggtttcactg ggtttttcct tgttattaac
     9601 taaagattat gatattatga atatgtaaaa aattaaaact cataaggaga gtattgtaat
     9661 gtcaattatt actgatgttt acgcacgcga agtccttgac tcacgcggta acccaacact
     9721 tgaagtagaa gtttatacag aatcaggtgc tttcggacgt ggtatggttc cttcaggagc
     9781 ttctactggt gaacacgaag cagttgaact tcgtgacggc gacaagtctc gttacggtgg
     9841 tcttggaaca caaaaagctg ttgacaacgt aaacaacgtt attgcagaag caatcattgg
     9901 ttatgatgta cgcgaccaac aagctatcga ccgtgctatg atcgctcttg acggaactcc
     9961 taataaaggt aaattaggtg caaatgctat tcttggtgtt tctattgccg tagcacgtgc
    10021 tgcagctgat taccttgaag taccacttta cagctacctt ggtggattca acactaaagt
    10081 tcttccaact ccaatgatga acatcatcaa cggtggttct cactcagacg ctccaatcgc
    10141 attccaagaa ttcatgatta tgccagttgg tgcaccaact ttcaaagaag cacttcgttg
    10201 gggtgctgaa gtattccatg ctcttaagaa aatccttaaa gaacgtggtc ttgaaacagc
    10261 tgttggtgat gaaggtggat ttgcacctaa atttgaaggt actgaagatg gtgtagaaac
    10321 tattcttaaa gctattgaag cagctggtta cgaagctggc gaaaatggaa tcatgattgg
    10381 ttttgactgt gcttcatcag agttctacga tgcagaacgt aaagtatacg actacggtaa
    10441 attcgaaggt gaaggtggag cagttcgtac tgctgcagaa caaatcgatt accttgaaga
    10501 attggttaac aaatacccaa tcatcactat tgaagatggt atggatgaaa acgactggga
    10561 tggttggaaa gctcttacag aacgccttgg tggacgtgta caattagttg gtgacgattt
    10621 cttcgttaca aatactgatt acttagcacg tggtatcaaa gaagaagctg ctaactcaat
    10681 ccttatcaaa gttaaccaaa tcggtacttt gacagaaact ttcgaagcca ttgaaatggc
    10741 taaagaagca ggttacactg cggttgtatc acaccgttca ggtgaaactg aagattcaac
    10801 aatcgctgat atcgcagttg caactaacgc tggtcaaatt aaaacaggtt cattatcacg
    10861 tactgaccgt attgctaaat acaaccaatt acttcgtatc gaagaccaac ttggtgaagt
    10921 tgctcaatac aaaggtatca aatcattcta caacttaaaa aaatagtctc agattattaa
    10981 acagttgaaa atataataaa aaccatcttg catatgcaag atggttttta tgtacacatt
    11041 ataataatag aattgatttg ataataaaca gttctaccat tgcaatccag cagcagcgat
    11101 ttctgctttt gaagcgtatt gtccatttgg acgatgccac ctccctctat agtcacgttt
    11161 gtagccttga ctagtttgtt cttgtactag agtttcttgt tttttctgag tttcagctgc
    11221 gagccttgct tgctcctctt cttgcttcct ttgagcttca gcagcaagcc ttgcttgttc
    11281 ttcttccagt tttttctgag tttcagcagc agctttttcc tcttcagctt tcttttctgc
    11341 aagtcgtttt tcttcttcgt gcttagcgat aagttttctt tgttctttag ctgataagac
    11401 agtattttga gcctttccgc tgaggtagtc aatggtagca tttggtgagg tattctctaa
    11461 ggtaacagtg ctgatgccga attgatcttg agtggatttg tttccgataa aaataggtaa
    11521 taatttccca gtcttatcaa taccaacata ttttaattca atttttcttg gaattagttc
    11581 attattttga taaattggtg ttactttata atctaagtag tagtttttat gagtcgaaag
    11641 ccaagtcttt agttgttttt catagaatag catgctctca gggttagatg aattcgtgct
    11701 attgtagttt ccagtattta gccaattcgt cattagaact aaattctttc tttcattgtt
    11761 tagtccacta aattggtggc aaataagacg gcctcggctc attagccaag ctttttgagt
    11821 accatcatta taatagaatt taaaattgtg ccatccaaca ggggttgttt ttaaacgttt
    11881 tactctcttt ttcttgggtt catctttaac ttttagctga atatgtgcga atgtggcacg
    11941 acctttgtca tccaactctc ctaaaaccaa ttgtcgtttg tgtttaaaag gtaaagtacc
    12001 attctctttt gttttatact cttgagcagt aacaatattt tctgtggtat gatttgtagc
    12061 taggtttatt ggtgtgtaaa gcgaaataac cgttagtaag gttgcaattc ctaattttat
    12121 aaactgtttt cttttcatct gttaacctct aaaataacat aaaatagtag ctgtttattt
    12181 ttaaatgcat tctgaaaatt aaaatgtgat ttttgtaaca atattataac ataatgatgt
    12241 atgacatgtg aagttaattt ataaaagatg ttacagctat atagaggagc tatagtataa
    12301 ataaaaaatt caaaatatag catatattat gatacaataa tagcaaaata gattttggga
    12361 gttatcatga aattactgac taatgcaaat actctcaagg gaaccatccg tgttcctggc
    12421 gataaatcaa taagtcacag ggctattatt tttgggagta tatctcaggg agttacccga
    12481 attgttgatg tcttaagagg cgaggatgtt ttatcaacta tagaggcttt taaacaaatg
    12541 ggggttctaa ttgaagacga tggcgagata atcactatct atggcaaagg atttgcaggt
    12601 ttaacacaac cgaataatct cttagacatg ggaaactctg gtacttcaat gcgtctgata
    12661 gcaggtgtgt tagctggtca agagtttgaa gtgacgatgg ttggagacaa tagtttgtca
    12721 aagagaccta tggatcgtat agcattgcct ttaagcaaaa tgggagctag aatttcggga
    12781 gtaactaacc gtgatttacc accattaaaa cttcaaggta caaaaaaatt aaaacctatt
    12841 ttttaccatt tacccgtagc ttctgcacaa gtaaaatcag ctttaatttt tgcggcttta
    12901 caaactaagg gagaatctct cattgtagaa aaagagcaaa caaggaatca tactgaggac
    12961 atgattcggc aatttggtgg acatttggat attaaagata aagaaattcg actcaatggc
    13021 ggtcagagct tagtaggaca agatataaga gttcctggtg atatttcgag tgctgccttt
    13081 tggatagtag cgggtcttat tattcctaac agtcatatta ttttagaaaa cgtaggtata
    13141 aacgagacac gaactggaat ccttgatgtc gtcagtaaaa tgggtggaaa aattaagtta
    13201 tcttcggttg ataatcaagt aaaatcagca acccttgcag ttgattactc tcatctacaa
    13261 gctactcata tttctggggc tatgattcca cgtctgattg atgaattacc tattattgct
    13321 cttttagcaa cacaagctca aggcaccaca gtcatagcag atgctcagga attaaaggtg
    13381 aaagagacag atcggattca ggtcgttgtc gaatcgctaa aacagatggg tgctgatatt
    13441 actgctacag cagatggtat gattatacgt ggtaataccc ctcttcacgc tgcttcactt
    13501 gattgtcatg gagatcatag aataggaatg atgatagcta ttgctgctct cttggtcaaa
    13561 gaaggcgaag tagatttatc tggtgaagaa gctattaata caagctatcc taacttttta
    13621 gaacatttgg agggattagt taatgcctaa ggtattattg ggattcatgg gagttggcaa
    13681 gactagtgtt gccaactgct tagaaaatga agtaattgat atggatagtc tgattgaaaa
    13741 acatattggt atgtcaattt cccgattttt tacagaagaa ggagaagcct cctttcgtgc
    13801 tttagaatct caatttttaa acgaactttt aaagaaaaaa aatgaaggac ttgtgatagc
    13861 tagtggagga ggtattgtac tccttgaaga aaatcgtcgt cttttaactt taaatcgtca
    13921 taataatatt cttttaacag gaagttttga agtcttatat catcgtataa aaaaagatga
    13981 gaagaataga agaccattat ttttaaacca ttcaaaagaa gaattttatg atatttatca
    14041 aaaacgcatg ttactctata gtggtttgtc tgatatgatt attgataccg attatcttac
    14101 accacaaaaa atagctactg taattggaga gtcactggtt aatttatcta actaagttat
    14161 cacagtttgg tagtatgaac tattattatt gaaagtaaat cacctgaaaa tatgtcaaga
    14221 aataattacg gtcagctaaa tcatcatgaa gagctacgat ataactatct tttaaaaaac
    14281 atccattatc ttaatgaacg tgaaaagatg gaatttcagt accttcatta taaaaaaaca
    14341 gcagtacgac cacagagaag gacagaaagt ccaccaacta attcttatta tgaggaacct
    14401 tattctgatt cttattatca agatgacgat ttttactcag agcctcagct aacctcacaa
    14461 ggtttaccaa tttatcaaga agagagagct ccgaagaaga aaaaacaaag ggccagaaaa
    14521 gaaaaacaga gggtgaaggt tatggcgcct ttccctccaa aggctataac cccccctaga
    14581 aagaaaaaaa aatttaaggg tttcctaaag ttcataggta ttatattact aattgtttta
    14641 tcaggtatgg ttttcatgtt tgttaaagga atgcgtgatg tgaataatgg gaagtcacat
    14701 tatagccctg caattattga agattttaag ggaaaagatg ctgttgatgg gactaatatc
    14761 ttaattttag gtagtgataa acgtgtcagc gagagatcta ctgatgcgag aacagatacc
    14821 ataatggttg ctaatgttgg taacaaggat aataaggtta agatggtgag ttttatgcga
    14881 gatctcttag tcaatattcc taattatagt acggaaggtt actatgatat gaaattaaat
    14941 gcgtctttta atttgggaga acaagataat cataaaggag cagagtatgt tcgacaaacg
    15001 ttgaaaaatc attttgatat tgatataaaa tattatgtta tggttgactt tgagacattt
    15061 gcagatgcta ttgatacact gtttcctaat ggtgtcaaga ttaatgctaa atttggactt
    15121 gtcggtggac aatctgctga ttcagttaaa gttcctgatg atttaaggat gaaaaatggc
    15181 gttgtaccta gccaaaaaat taaggttggg attcagtaca tggatggaag gacccttcta
    15241 aattatgctc gtttcagaaa agacgatgat ggggactttg gtcgaacaca acgtcaacaa
    15301 caagttatga gagctattgt ctctcaaata aaagacccac gtcgtctctt tacaggttct
    15361 gctgctatcg gtaaagccta tgctttaact tcaagtaatt tatcttatag ctttgtccta
    15421 acagatggca ttccaatttt atcagatgct aaaaatggta ttaagcaaat gacaattcca
    15481 agagaaggag attgggtaga tgattatgac cagtatggtg gtcaagggtt aacaattgat
    15541 tttgctaagt acaaaaagat cctaaagaaa atgggactgc gttaacattt ttaattatga
    15601 tataatagag tgatttattg tcactctatt tttatcttta gtgtataaac taaagacttc
    15661 aaatgataga tataaaagga aaattatgtt acataaaaat gatattattg aaactgaaat
    15721 tagcgacatt agccatgagg gaatgggtat tgccaaagta gatggctttg ttttctttgt
    15781 tgaaaatgct cttccaggtg agattattaa gatgcgcatc ttgaaattac gtaagcgtat
    15841 aggatatgga aaagttgagg aatatttaac gacttctcct caccgaaatg aaggattaga
    15901 ctatacctat ttacgtacag ggattgctga tttgggacat cttacttatg aacaacagct
    15961 gttatttaaa caaaaacaag tagctgataa cctttataaa attgctcata tatctgatgt
    16021 tttagtagaa cctactcttg gtatgacaat ccctttggca tatcgaaata aagctcaggt
    16081 accagttcgt cgtgttgatg gacagctaga gacaggtttt ttcagaaaaa attcacatac
    16141 tctggtatcc attgaagatt atttaattca ggaaaaagag attgatgctc tgattaattt
    16201 tacacgagat ttattaagaa aatttgatgt taaaccatat gacgaagagc agcaatctgg
    16261 tttaattaga aatctcgttg ttcgtcgagg acattataca gggcaattaa tgttggtttt
    16321 ggtgacaaca cgtcctaaaa tattccgcat tgaccaaatg attgaaaagt tggtatctgc
    16381 ctttccttct gttgtttcaa ttatgcaaaa tattaatgat cgtaatagca acgttatttt
    16441 tgggaaagaa tttcgtactc tatatggttc tgatactatt gaggaccaaa tgttaggaaa
    16501 tacttacgct atatcagccc aatcttttta ccaagttaat actgaaatgg ctgaaaaact
    16561 gtaccaaaaa gcaatcgatt tttcagattt aaactcagag gatatcgtta ttgatgccta
    16621 ttcaggtata ggaacaattg gcttatctgt tgctaaacaa gtaaaacatg tttatggtgt
    16681 tgaagttgtt gaaaaagcag taagtgatgc caaagaaaat gctactcgaa atgggataac
    16741 taacagtacc tatgttgctg attcggctga aaatgcaatg gcaaagtggc ttaaagaagg
    16801 tataaaacca actgttataa tggttgatcc accacgaaaa ggattaacgg agagttttgt
    16861 atattctgct gcccaaacaa aagcagataa aatcacttat atttcttgta attcagcgac
    16921 aatggcgcgt gatatcaaac tatttgaaga attagggtat catttagtaa aaattcaacc
    16981 agtagactta ttcccaatga cacatcatgt cgagtgtgtt gctctgcttg ttaaagccta
    17041 gaactctttg ataaatggaa atagtaaaaa gccttaattt taaggctttt tgtcgtatgt
    17101 aggggaagta tcagagtgag agtttaagct gagtatcttg atgtgatagc gtgattgaga
    17161 gagtatttat ttccataaac cctaggggta tggacttgtt tccatagaag aagagtttgt
    17221 ggaaataaac atacccccta aattattacc tagatggtcg ttga
//
LOCUS       DATRNK010000046        17094 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.046, whole genome shotgun sequence.
ACCESSION   DATRNK010000046 DATRNK010000000
VERSION     DATRNK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17094
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.046"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            101..1084
                     /gene="guaC"
                     /locus_tag="VBC52_001453"
     CDS             101..1084
                     /gene="guaC"
                     /locus_tag="VBC52_001453"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEO6883033.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIISSRSQADTSVKLGNYTFKLPVI
                     PANMQTIIDEEVAETLACEGYFYIMHRFNEEERKPFIKRMHDKGLIASISVGVKDYEY
                     DFVTSLKEDAPEFITIDIAHGHSNSVIEMIQHIKQELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCSKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHLESPGKLVEVDGQQFKEYYGSASEYQKGEHKNVEGKKIL
                     LPVKGRLEDTLTEMQQDLQSSISYAGGKELDSLRHVDYVIVKNSIWNGDSI"
     regulatory      1140..1235
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            1338..1919
                     /locus_tag="VBC52_001454"
     CDS             1338..1919
                     /locus_tag="VBC52_001454"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEO6883034.1"
                     /translation="MKLLEERILKDGDVLGENILKVDSFLTHQVDFELMQEIGKVFAD
                     KYKEAGITKVVTIEASGIAPAVYAAQALGVPMIFAKKAKNITMTEGILTAEVYSFTKQ
                     VTSQVSIVSRFLSNDDTVLIIDDFLANGQAAKGLLEIIGQAGAKVAGIGIVIEKSFQD
                     GRDLLEKTGVPVTSLARIKAFENGRVVFAEADA"
     gene            1919..3193
                     /locus_tag="VBC52_001455"
     CDS             1919..3193
                     /locus_tag="VBC52_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEO6883035.1"
                     /translation="MKFNEQSNSQAALLGLQHLLAMYAGSILVPIMIASALGYNAEQL
                     TYLIATDIFMCGIATLLQLQLSKHFGVGLPVVLGCAFQSVAPLSIIGAQQGSGYMFGA
                     LIASGIYVVLVAGIFSKVANFFPPIVTGSVITTIGLTLIPVAMGNMGDNAKEPSLQSL
                     TLSLVTIGVVLLINIFAKGFLKSISILIGLISGTILAAFMGLVDASVVAEAPLVHIPK
                     PFYFGAPRFEFTSILMMCIIATVSMVESTGVYLALSDITNDKLDSKRLRNGYRSEGLA
                     VLLGGLFNTFPYTGFSQNVGLVQISGIRTRKPIYFTALFLVILGLLPKFGAMAQMIPS
                     PVLGGAMLVLFGMVALQGMKMLNQVDFEHNEHNFIIAAVSIAAGVGFNGTNLFISLPN
                     TLQMFLTNGIVISTLTAVVLNIILNGLPKKLI"
     gene            complement(3292..4080)
                     /locus_tag="VBC52_001456"
     CDS             complement(3292..4080)
                     /locus_tag="VBC52_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEO6883036.1"
                     /translation="MSLFQEKIAYNCAKKEALYKESLGRYALRSMLAGAYLTMSTAAG
                     IVAADTIGKISPALSGFVFAFIFSFGLIYVLIFNGELATSNMLYLTAGAYNKNISWKK
                     AITILIYCTFFNLVGACILAWLFNQSYSFQHLTNDSFLGHVVAKKLGKPSSGAFLEGI
                     IANMFVNLAILAYMLLKEESAKMTVILSAIFMFVFLSNEHLIANFASFMLAAFSHIEH
                     IKGFTLLNVIRQWTLVFFGNWIGGGVFIGLAYAWLNKTKTSHHE"
     gene            complement(4179..5414)
                     /locus_tag="VBC52_001457"
     CDS             complement(4179..5414)
                     /locus_tag="VBC52_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6883037.1"
                     /translation="MKFVGIVGSNAEQSYNRMLLEFIRKNFKTKFELEVLEIDDIPMF
                     NQVQNWEESFQLRLLNNKITRADGVIIATPEHNHTITAALKSVLEWLSFAVHPLENKP
                     VMIVGASYYDQGTSRAQIHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDDNGNIINPG
                     TVQFLETCLDNFIKYVGVVSSLRKPKAIESEDLYCQGQIDTTITGIDPDDPDWLDKAA
                     EKVNAVEGDAYVKLDNGLLTVNQLNMFLNAMPFELTYADDNNQFLYYNNNHPDPDTML
                     GKRVREQVGNRMATVHGSLPPARMKNVEWVIGTLRNGNQEYVRTVVPGTPPEVINTHN
                     YQAMYYPDGSYAGINEIIFNFKPWLDWYLQTTGQRLVGGTTAAPGASAVDATSSASTS
                     TEDAPVDQATDATSGASEH"
     gene            complement(5438..6040)
                     /locus_tag="VBC52_001458"
     CDS             complement(5438..6040)
                     /locus_tag="VBC52_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6883038.1"
                     /translation="MKLIGIVGTNSNKSTNRQLLQYMQQHFADKAEIELIEVKDLPLF
                     NKPADKNVPQVILDIAAKIEEADGVIIGTPEYDHSIPSALMSVLAWLSYGIYPLLNKP
                     VMITGASYGTLGSSRAQLQLRQILNAPELKASVLPDEFLLSHSLQAFDKDGNLHDIET
                     SQKLDAIFDDFRLFVKITGKLSNARDLLQKEAENFDWESL"
     gene            complement(6058..6996)
                     /locus_tag="VBC52_001459"
     CDS             complement(6058..6996)
                     /locus_tag="VBC52_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEO6883039.1"
                     /translation="MQLSHSLRLMGTTIDIQINSKNAQKQIQEVIELLELYKNRFSAN
                     DFNSELMAINNNAGIKPIQVHPDLFELITIGKEHSLARPGNLNIAIGPLVQTWRIGFS
                     DAKLPSPSEISEAMILSDPTHILLDSKNQSVFLNQIGMKIDLGALAKGYIADKIIIYL
                     KNEMIDSAIINLGGNVLVHGDNPNRSEGYWVIGIQHPKKKRGQNIGAVKIKNQSVVTS
                     GTYERRLIIDDKEYHHIFDRQTGYPIQTEMASISIVSKQSVDCEIWTTRLFGLSIKEA
                     LDILNAVSYIEGIIITKDDRIYLSDGLKHHFQLFYH"
     gene            complement(7145..7327)
                     /locus_tag="VBC52_001460"
     CDS             complement(7145..7327)
                     /locus_tag="VBC52_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEO6883040.1"
                     /translation="MPFVKIDLFEGRSQEQKNELAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGELKKK"
     gene            7465..8034
                     /locus_tag="VBC52_001461"
     CDS             7465..8034
                     /locus_tag="VBC52_001461"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEO6883041.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSSLDTRD
                     EFGVVSSRIGMRREAVPISDDMDIFSYIQNLPQKPYCILIDECQFLSKKNVYDLARVV
                     DDLDVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPDI"
     gene            8069..9148
                     /gene="prfA"
                     /locus_tag="VBC52_001462"
     CDS             8069..9148
                     /gene="prfA"
                     /locus_tag="VBC52_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEO6883042.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQNISDAEEMIKDASGDAELEEMAKEELKESKAAKEEYEERLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYEI
                     DQKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTQKLEALNK"
     gene            9148..9978
                     /gene="prmC"
                     /locus_tag="VBC52_001463"
     CDS             9148..9978
                     /gene="prmC"
                     /locus_tag="VBC52_001463"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEO6883043.1"
                     /translation="MNYAQLIKHYGQLLEACGEEVENFIYVLKDLKQWSTTDYLLNQN
                     SSVSDTDQQLMENIFQQLKKHRSPQYITGKAYFRDLIFFVDERVLIPRPETEELVDLI
                     LSENKVEDCSVLDIGTGSGAIAISLKKERPSWDVLASDISVSALDLAKENANNCDAEV
                     TFIESDVFSNISGKFDIIVSNPPYISYNDKDEVGKNVLASEPHSALFADEEGLAIYRK
                     IIENSREYLQPRGKLYFEIGYKQGDDLRSLLKRYFPNNRCRVLKDIFGKDRMVVLDNE
                     "
     gene            9971..10567
                     /locus_tag="VBC52_001464"
     CDS             9971..10567
                     /locus_tag="VBC52_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEO6883044.1"
                     /translation="MNDLGQILEDHGAVIMPTETVYGIFAKALSEEAVNHVYELKKRP
                     RDKAMNLNICDFETILKYSKNQPTYLKQLYDAFLPGPLTIILEASQEVPHWINSGLLS
                     VGFRMPKHPVTLDMIANHGPLIGPSANISGCDSGRVFSEIQKQFNHQVLGIEDDKALT
                     GVDSTIIDLSGDRVKILRQGAITQEVLTATIPELIFEN"
     gene            10659..11915
                     /locus_tag="VBC52_001465"
     CDS             10659..11915
                     /locus_tag="VBC52_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEO6883045.1"
                     /translation="MIFDKDNFKEIDQELWQAIHDEEIRQQNNIELIASENVVSKAVM
                     AAQGSVLTNKYAEGYPSHRYYGGTDCVDVVESLAIERAKTLFNAEFANVQPHSGSQAN
                     AAAYMALIEPGDTVLGMDLAAGGHLTHGASVSFSGKTYHFVSYSVDPKTEMLDYDNIL
                     KIAQETQPKLIVAGASAYSRIIDFEKFRQIADAVDAYLMVDMAHIAGLVASGHHPSPI
                     PYAHVTTTTTHKTLRGPRGGLILTNDEAIAKKINSAVFPGLQGGPLEHVIAAKAVALK
                     EALDPSFKIYGEDIIKNAQAMAKVFKEDDDFHLISDGTDNHLFLVDVTKVIENGKKAQ
                     NVLEEVNITLNKNSIPFERLSPFKTSGIRIGTPAITSRGMGVEESRRIAELMIKALKN
                     HENQDILTEVRQEIKSLTDAFPLYEN"
     gene            11920..12897
                     /locus_tag="VBC52_001466"
     CDS             11920..12897
                     /locus_tag="VBC52_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEO6883046.1"
                     /translation="MMDFYIKQIIIHQFSPNDTELVLSDTPLTLTPRIDDYFRKKLSK
                     VFSDEAKRGYFGEDNVFMSHLQDDLYVSSCQIAQLWKEEFVISEDQKTNDLVFIQFDK
                     DGMEHFAFLRISLKEQFAHVSENQEQPITITQNNLPSAAQTPDEALVVNKSSKQYYLI
                     EKRIKHNGSFANYFSENLLQVQPEQSVKKSIKMVEQTAQKIAENFNKDDFSFQSKMKS
                     AIYKNLEEEQELSPEKLADQLFDDNLTARLTFVDELKDAIPGPVQVSDIDHSRQIKKL
                     ENQKLSLSNGIELIVPNNVYQDAESVEFIQNPDGTYSILIKNIQDIQNK"
     gene            12899..13501
                     /locus_tag="VBC52_001467"
     CDS             12899..13501
                     /locus_tag="VBC52_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEO6883047.1"
                     /translation="MFKFLKRLIALIIIIFIGYRLVIIHENVKKVLQYHDLVQNTLAE
                     NGSEANVHLVLSMIYTETKGDAIDVMQSSESISGTTNSITDSHTSIKHGVTLLSQNIS
                     QAKKAKVDVWTAVQAYNFGSSYIDYVADHGGENSIELAKNYSKNVVAPSLGNYNGDTY
                     FYYHPLALISGGKLYKNGGNIYYSREVQFNLYLIKIMELF"
     gene            13513..15234
                     /locus_tag="VBC52_001468"
     CDS             13513..15234
                     /locus_tag="VBC52_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6883048.1"
                     /translation="MKRLTYYFKGYIKETIFGPLFKLLEASFELLVPIVIAKMIDETI
                     PRGDRSSLLLQIGLIFFLAAVGVVVAITAQYYSSKAAVGYTRQLTEDLYQKVMSLGKK
                     DRDELGTASLITRLTADTFQIQTGLNQFLRLFLRAPIIVFGAIIMAFSISPSLTIWFL
                     VMVVTLFIIVFVMSRLLNPIYLKIRTSTDYLVKLTRQQLQGVRVIRAFNQVDRESEAF
                     NDINYHYTNLQLKAGRLSSLVTPLTFLVVNITLVVIIWRGNLNIANHLLSQGMLVALI
                     NYLLQILVELLKMTMLVTSLNQSYISAKRIIAVFERPSEIIDDKLEPKYSNKALEVQE
                     MAFSYPNSSEKALSDITFSMNVGETLGIIGGTGSGKSTLVNLLLHIYKVQEGDIDIYH
                     QGKSPDTISNWRTLVRVVPQNAQLFKGTIRSNLSLGLGKVSEEKLWTALEIAQASDFV
                     KEKDGQLDAPVESFGRNFSGGQRQRLTIARALVQDKIPFLILDDATSALDYLTETRLF
                     KAITKHFNQTNLIIVSQRINSIQNADRILLLDKGKQVGFDNHQSLLAHNKVYKSIYHS
                     QNFKEEE"
     gene            15235..16968
                     /locus_tag="VBC52_001469"
     CDS             15235..16968
                     /locus_tag="VBC52_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6883049.1"
                     /translation="MKASHPKKIQKKLVQDLLSKKSLVGMALLGTVVQVCLTVYLPVL
                     IGQAVDVVLSPHSMILLLPIMWKMIAVILANAIIQWINPLLYNRLIFHYVASLRKAVM
                     EKLNLLPIAYLDKRGIGDLISRVTTDTEQLSNGLLMVFNQFFVGLLTILVTIFSMAKI
                     DLLMLFLVLFLTPLSLFLARFIAKKSYHLYQNQTASRGRQTQFIEEMVSQESLIQAFS
                     AQEESSNHFRTINQEYANFSQSAIFYSSTVNPSTRFINSLIYGFLAGIGALRIMSGAF
                     SVGQLITFLNYVNQYTKPFNDISSVLSEMQSALACAERLYSILEESSPNITGTEKLDS
                     STVKGQIDFKNVVFGYNKSKPLLNGINLHIPAGAKVAIVGPTGAGKSTLINLLMRFYE
                     VDGGNILLDGKPITDYEPSQLRQEIGMVLQETWLKSATIHDNIAYANPKASRDEVIEA
                     AKAANADFFIKQLPNGYDTYLEDAGDSLSQGQCQLLTIARIFLKLPRILILDEATSSI
                     DTRTEVLVQEAFQMLMKGRTSFIIAHRLSTIQTADIILVMVSGEIVEVGNHSELIAQK
                     GIYYQMQNAQK"
BASE COUNT         5547 a         2686 c         3261 g         5600 t
ORIGIN      
        1 ataaagtcta taatttaaaa aggaaaggta tgtttttacc tagcgatgct ttgttataca
       61 aagttatagg aaggtcattt tactcattag gagaaccatt atgtttaacg atattcctgt
      121 ttttgattat gaagatattc aattaatacc aaacaagtgt attatatcta gccgctcaca
      181 agctgacaca agtgtcaaac ttggaaacta tacctttaaa cttccagtaa tccctgctaa
      241 tatgcagact attattgatg aagaggtagc tgaaacatta gcgtgtgaag gttattttta
      301 tattatgcac cgttttaatg aagaagaaag aaaacctttt atcaagcgta tgcatgataa
      361 aggtttaatt gcatcgattt cggttggggt caaagactat gaatatgact tcgtgacaag
      421 tctgaaagaa gatgctcctg agtttatcac tattgatatt gcacatggcc attctaacag
      481 tgttatagag atgatccaac acattaaaca agagcttcca gaaactttcg ttattgctgg
      541 aaatgtagga acgcctgaag ctgttagaga attggaaaat gcaggtgcag atgcaactaa
      601 agttggtatt ggacctggta aagtttgtat tactaaagtc aagacaggtt ttggtactgg
      661 aggttggcaa ttagctgcct tacgttggtg ttctaaagct gctcgcaagc caattattgc
      721 tgatggtggt attcgaacac atggggatat tgcaaaatct atccgctttg gtgctagtat
      781 ggtaatgatt ggttcgctgt ttgctggtca ccttgaaagt cctggaaaac ttgtggaagt
      841 agatggacag caatttaaag aatactatgg ctcagcttct gaatatcaaa agggagaaca
      901 taaaaatgtt gaaggtaaga aaattctatt acctgttaaa gggcgactag aggatacttt
      961 gacagaaatg caacaagatt tgcaaagttc tatttcttac gcaggaggaa aagaattgga
     1021 tagcttgaga cacgttgatt acgttattgt taaaaattct atttggaatg gtgattccat
     1081 ttaaattaaa tcttgaaatc ttaattaaaa atgttacaat agttatctat catacataac
     1141 aattaaatat atgatttact tatttatgct gaggattggc ttagcgtctc tacaagacac
     1201 cgtaatgtct aacaataagt aagctaataa atagcttgcc tgttggttca tgttaatgaa
     1261 gcgataaatt agaggagatt tgtgtcctct gattgaggta agttattttt tattttctaa
     1321 agagtgaagg ggttattatg aagttattgg aagaacgtat tcttaaagat ggtgatgttt
     1381 taggtgagaa cattttaaag gttgattctt ttttgactca tcaggtagat tttgagttaa
     1441 tgcaggaaat aggtaaagtt tttgctgata aatataaaga agccggcatt acgaaggttg
     1501 ttacgattga agcatctgga attgcaccag cagtgtacgc agctcaagca ttgggcgtac
     1561 caatgatatt tgctaaaaaa gctaagaaca ttactatgac tgaaggtatc ttaactgctg
     1621 aagtgtattc ttttacaaag caagttacga gtcaagtttc tattgtgagt cgctttttat
     1681 ctaacgatga tactgtactc atcattgatg actttttagc aaacggtcaa gcggctaaag
     1741 gattacttga aattattggt caagctggag ctaaggttgc tggtatcgga atcgttattg
     1801 aaaaatcttt ccaagatggg cgtgatttgt tagaaaaaac aggtgttcca gttacttctc
     1861 ttgctcgtat taaagcgttt gaaaatggtc gagttgtttt tgcggaggct gatgcttaat
     1921 gaaatttaat gagcaaagta actcgcaagc agcacttcta ggcttgcaac acctgttagc
     1981 tatgtatgca ggatccattc ttgtacctat catgattgct agtgctcttg gttataatgc
     2041 tgagcaacta acttatctta ttgcgacaga tatttttatg tgtgggattg ccaccttatt
     2101 acaattacaa ttaagtaagc actttggagt gggtcttcca gttgtattag ggtgtgcctt
     2161 tcagtctgta gctcctttat caattattgg tgcacaacaa ggttccggct atatgtttgg
     2221 agctttaatt gcttcaggaa tatatgtcgt tttagttgct ggcatttttt ctaaagttgc
     2281 aaatttcttt cctccaattg taacaggatc agttattact acaattggtt tgacattaat
     2341 accagttgcg atgggaaata tgggtgataa tgcaaaagaa ccgagcttac aatctttaac
     2401 tttatcactg gtaacgattg gtgttgtttt attaattaat attttcgcaa aaggcttttt
     2461 gaagtcaatt tcaatcctta ttggactcat atcaggtaca attcttgcag catttatggg
     2521 cttagttgat gcttctgtgg tagcagaagc accacttgta catattccga agccatttta
     2581 ttttggagct cctagatttg aatttacttc tattttaatg atgtgtatta ttgcaacagt
     2641 ttctatggta gaatcaacag gtgtttacct tgcgctttca gatattacaa acgataaatt
     2701 agacagtaag agacttcgta atggttaccg ttcagaagga ttggcagtat tacttggcgg
     2761 cttatttaat acctttccat ataccggttt ttctcaaaat gtgggactgg tacagatatc
     2821 tgggatacgt acgcgtaagc cgatatattt tacagctctc ttcttagtta tacttggctt
     2881 gttacctaaa tttggcgcaa tggctcaaat gattccaagt ccagttcttg gtggtgctat
     2941 gttagtttta tttggtatgg tagcacttca agggatgaaa atgcttaatc aggttgattt
     3001 tgagcataat gagcataact ttatcattgc agccgtatca attgcagcag gagtaggttt
     3061 taatgggaca aatctcttca ttagtcttcc taatacttta caaatgtttt tgacaaatgg
     3121 tattgttatc tcaaccctga cagctgttgt tttaaatatc attttgaatg gattgcctaa
     3181 aaaattaata tgatactttt ctttatttat caaaaaagtt aatttttagt tattgatttc
     3241 atgatgagaa tataaaagaa aaactgagta ataaattact cagtttttct tttattcatg
     3301 gtgagaagtt tttgttttat ttaaccaagc ataggctagg ccgatgaaaa cgccccctcc
     3361 tatccagtta ccaaaaaata caagtgtcca ttggcggata acgtttaaaa gagtaaaacc
     3421 tttaatatgt tcgatatggc taaaagcagc taacataaag gatgcaaaat tagcaattaa
     3481 gtgttcgttt gatagaaaaa caaacataaa aatagcagat aaaataaccg tcatttttgc
     3541 cgattcttct tttaataaca tgtaagcaag gatagctaaa ttcacaaaca tattagcaat
     3601 aataccttct aggaaagctc ctgaactagg ttttcctaac tttttagcaa cgacatgccc
     3661 taaaaagcta tcatttgtta aatgctggaa tgagtacgat tgattaaaca accaagctaa
     3721 tatacaagca ccaacgaggt tgaaaaaagt acaataaatt aaaattgtta tggctttttt
     3781 ccaagagata tttttattat aggctcctgc agtgagataa agcatattag atgtcgccaa
     3841 ttcaccatta aatattaaaa cataaataag tccaaaacta aagatgaaag caaatacaaa
     3901 acctgataga gcaggagaaa ttttaccaat agtatcagct gcgacgatac cggcagcagt
     3961 actcattgtc aaataagccc ctgctagcat tgatctcaag gcgtagcgtc ctaaactctc
     4021 tttataaagc gcttcctttt tagcgcaatt gtaagcaatt ttttcttgaa aaagtgacat
     4081 agtatattcc ttttatgtga ttttattaac ttgattaaat cttaaaaagg aggatcttga
     4141 tatcttttta aggtatcaga tcctcaaaag cgaggagatt aatgttcaga ggcaccagaa
     4201 gtggcatctg tggcttgatc aacgggagca tcctctgtgc tagttgaagc gctagaagta
     4261 gcgtcaactg ctgaagctcc aggagcagca gtagttccac cgaccaaacg ttgtccagta
     4321 gtttggaggt accaatctaa ccacggttta aagttaaaga taatttcatt aataccagca
     4381 taagatccgt ctgggtagta catagcttga tagttgtgag tattgataac ttctggaggt
     4441 gtaccaggaa cgactgtacg tacatattct tgatttccat tacgaagagt accgataacc
     4501 cattcaacat ttttcattcg tgccggtggt aaactaccat gaactgtggc catacggttt
     4561 cctacttgtt cacgaacacg ttttcctaac attgtatctg gatctggatg attattattg
     4621 taatatagaa attgattatt atcatctgca tatgttagct caaatggcat agcatttaaa
     4681 aacatattaa gttggttaac agtaagtaaa ccattgtcta gtttaacgta agcatcccct
     4741 tcaacggcgt taactttctc ggctgccttg tctaaccaat caggatcatc tggatcgata
     4801 ccagtaatgg ttgtatcgat ttgtccctga cagtagaggt cttcagattc aatagcttta
     4861 ggttttctca atgatgatac aactcctaca tatttgataa aattatcaag acaagtttct
     4921 aaaaattgaa cagtcccagg attgataatg tttccattat catcaaaggc ttctttagct
     4981 tttcctaata agaattcatt tcctggaagt gtgtaagcat taacgccagg agcatcaagg
     5041 attttacgaa ggtgtatttg agcacgtgat gtgccttgat cataatatga agcaccgaca
     5101 atcataacag gtttattttc gagtggatgc acagcaaatg agagccactc aagcacactt
     5161 ttaagagcgg ccgtaatagt atgattatgt tcaggagtgg caataataac gccatcagct
     5221 cgtgtgatct tattattaag aagacgaagt tggaaacttt cttcccaatt ttggacttga
     5281 ttaaacattg gaatatcatc gatttcaagg acttccaatt caaatttggt tttaaaattc
     5341 ttgcgaataa attcaagcaa catacgatta tatgattgct ctgcatttga gccaacaata
     5401 cctacaaatt tcatcttagt tattcccttc tatactctta taaactttcc cagtcaaaat
     5461 tttctgcttc tttttgaagt aaatcacgag catttgataa tttaccagta atcttaacaa
     5521 aaagacgaaa atcatcaaag atagcatcta gcttctgact tgtttcgata tcgtgtaggt
     5581 tgccatcttt atcaaaggct tggagagagt gtgataacaa gaattcgtca ggtaaaacac
     5641 ttgcttttag ttcaggagcg ttaagtattt gacgtaattg taattgtgca cgtgatgatc
     5701 caagtgttcc atatgaagca ccagtaatca taacgggttt gttgagaagt gggtaaatac
     5761 catatgatag ccaagctaaa acactcatta aggccgaagg tatagaatga tcatattctg
     5821 gcgtaccaat aataacacca tcagcttctt caattttagc agcaatatct aaaattactt
     5881 ggggaacatt tttatcagca ggtttattaa ataacggaag gtctttaact tcgatgagtt
     5941 caatttctgc tttgtcagca aagtgttgtt gcatgtattg taataattga cgatttgttg
     6001 atttatttga gttagttcca acgatgccaa taagtttcat attagaaatc ctctttctta
     6061 atgataaaat agttgaaaat gatgttttaa gccatcagat aaatagatac gatcatcttt
     6121 agtgatgata atgccttcaa tgtaggaaac agcgtttaaa atatcaagag cctctttaat
     6181 ggataagcca aacaaccttg tggtccagat ttcacagtcg acggattgtt tagaaacaat
     6241 agaaatgcta gccatctctg tttgtatcgg ataaccagtc tgtcgatcaa aaatatgatg
     6301 gtattcctta tcgtcaataa ttagacggcg ttcataagta cctgaggtta caacagattg
     6361 gtttttaatt ttgacagctc caatattttg tccccgtttc tttttcggat gttgaatacc
     6421 gataacccaa tacccttctg aacgattagg attatctccg tgaactaaaa cgtttccgcc
     6481 taagttaatg atagcagaat caatcatctc atttttgaga tagattataa ttttgtcagc
     6541 gatatatcct tttgcaagtg cacccaaatc aattttcatt cctatctgat ttaaaaaaac
     6601 agattgattt tttgaatcta gaagtatatg agttgggtca gataaaatca tagcttctga
     6661 tatttcacta gggctaggta gtttagcatc tgaaaatcca attctccaag tttgcactaa
     6721 aggaccaata gcaatattta ggttaccagg cctagctaag ctgtgctctt tcccaattgt
     6781 gattaattca aagaggtctg gatgtacttg aataggcttg ataccagcgt tattatttat
     6841 agccattaac tctgaattaa agtcatttgc actaaagcga tttttgtaga gctccaagag
     6901 ttctatgacc tcttggattt gtttttgagc atttttagaa ttaatttgaa tatcgatagt
     6961 cgttcccatg agtcttaaac tatgagaaag ttgcaaattt tccaccccca aagcaatata
     7021 ttctaatgat accgttttct aacttgaaaa tcaatactta ttttcaaata ttttcaattt
     7081 gtcaagaaat atgcttgcaa agactatcaa aagaggttac ctgggtaacc tcttttacta
     7141 tttcttattt tttcttaagt tcaccttgag gatagtaggt tccttcaggc atatcgttga
     7201 taaaaacatg gatattttct ttaggtgctt ttgcaatgcg tgatactact tcagtaacct
     7261 cgcgagctaa ttcatttttt tgttcttgtg agcgtccctc aaacaagtca atttttacaa
     7321 atggcataat gttactcctt tatgttattg ggcatcattg taacataatg ttgtgaaaaa
     7381 gtacacactg ttttcaatgg ctgggaaaac ttcagccttt gtggtaaaat aggtagaata
     7441 ctatagaaag taggattttc cgctttggct caactctatt ataaatatgg gacaatgaat
     7501 tctggtaaaa ccattgaaat tttaaaagtt gctcataact atgaagaaca aggaaaacct
     7561 gtggttatta tgacaagctc cttggatact cgagatgaat ttggagtcgt ttcaagtcgt
     7621 attggaatgc gtcgtgaagc agttccaatt tcagatgata tggacatttt ctcctatatt
     7681 cagaatttac cacaaaagcc ttattgtatt ttaattgatg aatgtcaatt tttgagtaag
     7741 aaaaacgtct acgatttagc tagagtagta gatgacttag atgtaccagt catggctttt
     7801 ggtttgaaaa atgatttcca aaacaatctt tttgaaggtt caaaacattt gctattattg
     7861 gccgataaga ttgatgaaat caaaacaatt tgccaatact gtagtaagaa agcaaccatg
     7921 gttttaagga cagaaaacgg aaaacctgtc tatgaaggtg atcagataca aattggtggt
     7981 aatgaaacat atatcccagt ttgtcgaaaa cattatttta atccagatat ttaaaagata
     8041 atcggtttta aaggagatac aaagaaaaat gaatatttat gatcaattgc aggcagttga
     8101 agatcgttat gaagagttag gtgagttact tagtgatcca gatgttgttt cagatacaaa
     8161 gcgatttatg gaattatcac gtgaagaagc taatactcga gaaactgtca cagcttatag
     8221 agaatataag caagtcattc aaaatatttc agatgctgaa gaaatgatca aggatgcttc
     8281 tggtgatgct gaattggaag aaatggctaa agaagaactt aaagaatcaa aagcagctaa
     8341 agaagagtat gaagaaagat taaaaatcct tctattacct aaagatccta acgatgataa
     8401 aaacattatt ttagaaattc gtggtgctgc tggtggcgat gaagcagcgc tttttgctgg
     8461 tgatctttta acaatgtatc aaaaatatgc tgaaactcaa ggatggcgtt ttgaagttat
     8521 ggaatcatcc gttaatggtg ttggtggtat taaagaagtg gttgctatgg tttctggtca
     8581 gtcagtttat tctaagctta aatatgaatc aggagctcat cgtgttcaac gtgtcccggt
     8641 gacagagagt cagggtcgtg ttcatacttc aactgcaact gttttggtaa tgccagaagt
     8701 tgaagaagta gaatacgaaa ttgatcaaaa agaccttcgc gtcgatattt accacgcgtc
     8761 tggtgctggt ggtcagaacg tcaataaagt tgctactgca gttcgtatgg tacacattcc
     8821 tacaggaatc aaagttgaaa tgcaagaaga acgtactcag caaaaaaacc gtgataaagc
     8881 gatgaaaatc attcgtgctc gtgttgctga ccattttgcg caaattgctc aagatgaaca
     8941 agatgctgaa cgtaaatcaa cagttggtac tggtgatcgt tcagaacgta ttcgcactta
     9001 taatttccca caaaatcgtg taacagatca tcgtattggt ttgacacttc aaaaattaga
     9061 tactatttta tctggaaaaa tggatgaagt tattgatgca cttgttatgt atgatcagac
     9121 tcaaaagtta gaagccttaa ataaataatg aattatgcgc agttaatcaa gcattacgga
     9181 cagttattag aagcttgtgg tgaagaagtc gaaaatttta tttatgtatt aaaagaccta
     9241 aaacaatggt caacaactga ttacctttta aatcaaaata gtagtgtgag tgatactgat
     9301 cagcaattaa tggaaaatat ttttcaacag cttaaaaaac atagatcacc gcagtatatt
     9361 acaggtaaag cttactttag agaccttatt ttctttgttg atgaacgagt tttaattccg
     9421 aggcctgaaa cagaggagtt ggttgatcta atcttatcag agaataaagt agaagattgt
     9481 tctgttttag atattggtac aggtagtgga gcaattgcca tttctctaaa aaaagaacgc
     9541 ccctcttggg atgttctagc tagtgacatt tctgtatctg cattagactt ggcaaaggaa
     9601 aatgctaata attgtgatgc ggaagtgact tttatagaat cagatgtctt tagtaacatt
     9661 tcaggtaaat ttgatattat tgtgtctaat cctccctata tttcttacaa tgacaaagac
     9721 gaggtgggga agaatgtgct agcttcagag cctcatagtg ccctttttgc agacgaagaa
     9781 ggattagcta tttatcgtaa aatcattgaa aactcaagag aatatttgca acctagaggt
     9841 aaattatatt ttgaaatagg ttacaaacaa ggtgatgatt tacgaagttt attgaaaaga
     9901 tattttccaa acaatcgttg cagagttttg aaagatatat ttggtaaaga tagaatggtg
     9961 gtattagata atgaatgatt tgggacagat tttagaggat catggcgcag ttattatgcc
    10021 aacagaaaca gtctacggaa tttttgccaa agctttgagt gaagaagctg tgaaccacgt
    10081 ctatgagctt aaaaaaaggc ctcgtgataa ggctatgaac ttaaatatat gtgattttga
    10141 gactatttta aaatattcta aaaatcaacc gacctattta aagcaattgt acgatgcttt
    10201 tttaccagga ccgctaacca ttattttgga agcttcacaa gaagttccgc attggatcaa
    10261 ttctgggtta ttaagcgtag gatttcggat gcctaagcac cctgtaaccc ttgatatgat
    10321 tgctaaccat ggtccactga ttggtccttc tgctaatatt tctggatgtg atagtggtcg
    10381 tgtgttctca gaaattcaaa aacagtttaa tcatcaagtt ttaggtattg aagatgataa
    10441 agcattgaca ggtgtggatt ctactattat tgatttgtct ggtgatagag ttaagatttt
    10501 aaggcaaggt gcaatcacgc aagaggtact aacggctacg atacctgaat taatttttga
    10561 aaattaattt aatcatcttg caactaatag taaaaatgtg ataaatataa tgattgaaac
    10621 cattatttaa aagttataac gagcttaagg agtaaaaaat gatttttgat aaagacaatt
    10681 ttaaagaaat tgaccaagaa ctttggcagg ctattcatga tgaggaaatt cgccaacaaa
    10741 ataatattga attaattgca tcagaaaacg ttgtttcaaa agctgttatg gcagcacaag
    10801 gttctgtttt gacaaataaa tatgccgaag ggtatcctag tcatcgttat tatggaggaa
    10861 cagattgtgt tgatgtagtt gaaagtttag ctatagaacg agctaagacg ttatttaatg
    10921 ctgagtttgc taatgttcaa ccacattctg gtagtcaggc taatgcagca gcttacatgg
    10981 ctttaatcga gccgggagat actgtcttag ggatggactt agcagcaggt gggcacttaa
    11041 cacatggcgc ttcagttagc ttctcaggaa aaacttatca ttttgtttct tattctgttg
    11101 atcctaagac ggagatgcta gactatgata acattttgaa aatagcacag gaaacgcagc
    11161 ctaaattaat tgttgcaggg gcatcggctt attctcgcat tattgatttt gaaaaatttc
    11221 gtcaaatagc agacgcagta gatgcttact taatggttga tatggctcac attgcaggtt
    11281 tagttgcttc tggtcatcac cctagtccta ttccttatgc tcatgtaacg acaacgacaa
    11341 cacataagac tctacgtgga cctagaggtg gtttgatttt aaccaatgat gaagcaattg
    11401 ctaaaaaaat taattctgca gtctttccag gactccaagg tggaccatta gaacatgtaa
    11461 ttgctgcaaa agctgtagct ttgaaagaag ccctagatcc ttcctttaaa atatacggtg
    11521 aggatattat taaaaatgct caagccatgg ctaaagtttt taaagaagat gatgactttc
    11581 atttgatttc tgatggtact gataatcatt tgttcttagt agacgtcaca aaagttattg
    11641 agaatggcaa aaaggcacaa aatgttcttg aagaagtcaa tatcactctt aacaagaact
    11701 ctataccgtt tgagcgacta tcaccattta aaacatcagg tattcgaatt ggtactccag
    11761 caattactag tcgaggaatg ggagttgaag aatctcgaag aatcgctgaa ttaatgatta
    11821 aggcactcaa aaatcatgaa aatcaagata tcctcacaga agttcgtcaa gaaattaaat
    11881 cattaacgga cgctttcccc ttgtatgaga attaatgtta tgatggattt ttatatcaaa
    11941 cagattatta tacatcagtt ttctcctaac gacacagagt tagtgctatc agatactccg
    12001 ttaaccctaa ctccaaggat tgatgactac tttcgtaaaa aattaagtaa agttttttct
    12061 gatgaagcta agcgtggcta ttttggagag gataatgtat ttatgagtca tttacaagat
    12121 gatttatatg tttcttcctg tcaaattgcg caactttgga aagaagagtt tgtcatatcg
    12181 gaagatcaaa aaacgaatga cttagttttt attcaatttg acaaggatgg aatggaacat
    12241 tttgcttttc ttcgaatctc attaaaggaa caatttgctc atgtttctga aaatcaagag
    12301 cagccaatta caataacaca gaataacctt ccaagtgctg ctcaaactcc agatgaagct
    12361 ttggttgtta acaagtcatc aaagcaatac tatttaattg aaaagcgtat caaacacaat
    12421 ggttcatttg ctaattattt ttctgaaaat ttattacaag tacaaccaga acagtcggtt
    12481 aaaaaatcta tcaaaatggt tgaacagact gctcaaaaaa ttgctgaaaa tttcaacaaa
    12541 gatgattttt ctttccaatc aaaaatgaaa tcggctattt ataagaattt agaagaagag
    12601 caagaattgt ctccagaaaa attagcagat caattatttg atgataattt aacagccagg
    12661 ctgacttttg ttgatgaatt gaaagatgca atacctggac ctgttcaagt aagtgatatt
    12721 gatcattccc gtcaaattaa gaagttagag aatcagaagc tatctctttc aaatggtatt
    12781 gaattgattg tacccaacaa tgtctaccaa gatgccgaat cagttgaatt tatccaaaac
    12841 cctgatggta catactcaat actaatcaaa aacatacagg atattcaaaa taaataatat
    12901 gtttaaattt ttaaaacgtt tgatagcatt aattattatt attttcattg ggtaccgtct
    12961 ggttatcatc catgaaaatg ttaaaaaagt gcttcaatat catgatttag tgcagaatac
    13021 tcttgcagag aatggttcag aggctaatgt gcacttagta ttatctatga tttatactga
    13081 aactaaggga gatgctatag atgtgatgca atcaagtgaa agtatcagtg gaactacgaa
    13141 cagtattacg gatagccaca ccagtattaa acatggcgtc actctgttgt cacaaaatat
    13201 aagtcaagct aaaaaagcta aggttgatgt atggactgct gtccaagcat acaattttgg
    13261 ttcttcttat attgattatg tagcggatca tgggggtgaa aattccattg agttggccaa
    13321 aaattactct aaaaatgttg tagctcccag cctaggaaat tataacgggg acacttactt
    13381 ttactatcat cctctcgcct taatttcagg agggaagctc tataaaaatg gtggtaatat
    13441 ttactactca cgtgaagttc aattcaacct ttatttgata aaaataatgg agttgtttta
    13501 agaagggtaa gtatgaaaag actaacttat tattttaagg ggtatatcaa agaaactatc
    13561 tttggacccc ttttcaaatt attagaagcc tcttttgaac ttttggtacc aatcgttatt
    13621 gcaaaaatga ttgacgagac cattccacga ggggatagaa gtagtttact gttgcaaatt
    13681 ggattgattt tctttttggc tgcggtgggt gttgtagtag cgataactgc tcaatattat
    13741 tcttcaaaag ccgcagttgg ttatacgaga caattgacag aggatctcta ccaaaaagtc
    13801 atgtcactgg ggaaaaaaga cagagatgaa ttgggaactg ctagtttgat tactcgtttg
    13861 actgctgata catttcaaat ccaaactggt ttaaatcaat ttttacgttt atttttgagg
    13921 gctccgatta ttgtttttgg tgccataatt atggcttttt ccattagccc ctcattgact
    13981 atttggttct tggtaatggt tgtcacgtta tttatcattg tttttgtaat gtctcgacta
    14041 ttaaatccta tttaccttaa aatcagaact tcaaccgatt atttggtaaa gttgactagg
    14101 caacaacttc aaggtgtacg agttatccgt gcctttaatc aagtagatag agagagtgaa
    14161 gcatttaatg atatcaatta tcattatacg aatttacaat taaaagcagg taggctttct
    14221 agtttagtaa cgcctctaac atttctagtt gttaatatca ctttggttgt gattatttgg
    14281 cgtggtaatt taaatatagc taatcatctt ttatcacaag gaatgttggt tgctttaatt
    14341 aattacttat tgcaaattct tgttgaattg ttaaaaatga caatgttggt gacatcactg
    14401 aaccaaagtt atatcagtgc taagcgaatt atagccgttt ttgaaagacc gtctgaaata
    14461 attgatgaca aacttgagcc aaaatattcg aataaggctt tagaagtaca agaaatggca
    14521 ttttcttatc caaattcttc tgaaaaagct ttatctgata ttactttttc tatgaatgta
    14581 ggagaaactt tagggataat tggtggaact ggctctggga aatcaacctt ggttaattta
    14641 ctgcttcata tttataaagt gcaagaaggg gatattgata tatatcatca gggaaaaagt
    14701 ccagatacaa tttcaaattg gcgtaccctg gtaagagttg ttcctcaaaa tgctcagttg
    14761 tttaaaggaa ctattcgttc taacctttct ttgggacttg gtaaagttag tgaggaaaaa
    14821 ctttggactg ctttagaaat agcacaagct agtgattttg taaaagaaaa agatggtcaa
    14881 cttgatgccc ctgtagagag ttttggtaga aatttctctg gtggtcaaag gcaaaggttg
    14941 acgattgcaa gagctttagt tcaagataag ataccatttt tgattttaga cgatgcaaca
    15001 tctgcattgg attatttaac agaaactcgt ttatttaaag ccataactaa acatttcaac
    15061 caaactaatc ttattattgt atcacagaga attaatagta tacaaaatgc agatagaatc
    15121 ttactccttg ataaggggaa acaagttggt tttgataatc atcaatcttt attagctcat
    15181 aacaaagttt ataagtccat ttaccattca caaaatttta aggaggagga gtaaatgaaa
    15241 gcaagtcatc ctaaaaagat acaaaaaaag cttgttcaag atcttctttc taaaaaaagc
    15301 ttagtaggaa tggcattatt agggacggtt gtacaagttt gcctcactgt ttatttgcct
    15361 gttctaattg gacaagcggt agacgttgta ttatcgcctc attctatgat attattgtta
    15421 cctataatgt ggaagatgat tgcagtcatt ttagcaaatg ctattattca atggattaat
    15481 cctctgctct ataaccgctt aatttttcat tatgttgctt ctttaagaaa ggctgtaatg
    15541 gaaaagttga atctattgcc tatagcttac cttgataaac gtggcatagg tgacttgatt
    15601 agtcgtgtga ctaccgatac ggagcaatta tcaaatggtt tattaatggt ttttaaccag
    15661 ttctttgttg gtttattaac tattctagtt actattttta gtatggcaaa gattgattta
    15721 ttaatgcttt ttttggtatt atttctgacg cctttatcac tctttctagc ccgctttatt
    15781 gctaaaaaga gttatcactt atatcaaaat caaacggcat cacgaggacg acaaactcaa
    15841 ttcattgaag agatggtgag tcaagaaagc cttattcaag cattcagtgc gcaagaagaa
    15901 agtagtaatc attttaggac tattaatcaa gagtatgcta acttttctca atcagcaatt
    15961 ttttattctt caactgtcaa tccatcaacc cgatttatca atagtttgat ttatggcttc
    16021 ttagctggga ttggtgccct tcgtattatg tcaggggctt ttagtgttgg tcaattaatc
    16081 actttcttaa attatgtcaa tcaatatact aagccattta atgacatctc ttccgtcctg
    16141 tctgagatgc aaagtgctct ggcttgtgca gaacgccttt actctatttt agaagaatcg
    16201 tcaccaaata taacagggac agaaaaactt gattcatcaa cggtcaaggg tcaaatcgat
    16261 tttaaaaatg ttgtttttgg ttataataaa tcaaaaccac tattgaatgg tattaattta
    16321 cacattccag caggagctaa ggtcgctatt gtaggaccaa caggtgctgg gaagtctact
    16381 ttaatcaatt tattaatgcg tttctatgaa gttgacgggg gaaatatcct tttagatggt
    16441 aagcctatta ctgattatga gccatcacaa ttaaggcaag aaattggaat ggtattacaa
    16501 gaaacgtggt taaaaagtgc tacgattcac gataacattg catatgccaa tcctaaagct
    16561 agccgtgacg aagtgataga ggctgcaaaa gcagctaatg cagatttctt tatcaaacaa
    16621 ttaccgaatg gatatgatac gtatttagaa gatgctggag actcgttatc acaggggcaa
    16681 tgtcagctat tgacaattgc tcgtattttt cttaaattac ctagaatatt gatattagat
    16741 gaggcaacat cgtctataga tactcgtact gaagttcttg ttcaagaagc attccaaatg
    16801 ttgatgaagg ggagaacaag ctttattatt gcacatcgct tatctaccat tcaaacagca
    16861 gatattattt tagttatggt atctggtgaa attgtagaag tgggtaacca ctctgaatta
    16921 atagctcaaa aaggtattta ttatcaaatg caaaatgcac aaaagtagta agaggaatta
    16981 tatgaaaaaa aggcttcaac tattattact gatatttatt tctgcagttt catggtttat
    17041 cagtaaccaa ctagcaacta agaatacggt attatctgtt ttaataggtt tagt
//
LOCUS       DATRNK010000047        16292 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.047, whole genome shotgun sequence.
ACCESSION   DATRNK010000047 DATRNK010000000
VERSION     DATRNK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16292
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.047"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(260..550)
                     /locus_tag="VBC52_001470"
     CDS             complement(260..550)
                     /locus_tag="VBC52_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cuticle protein"
                     /protein_id="HEO6883050.1"
                     /translation="MKLKISVLTAASAVTLLLATLASLAKADTVSYPSRDAGFVYSRG
                     YAPSESSYPSRDAGFIYSRGYASSESSYPSRDAGFIYSRGYAPKHFLSAYDK"
     gene            complement(892..1632)
                     /locus_tag="VBC52_001471"
     CDS             complement(892..1632)
                     /locus_tag="VBC52_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6883051.1"
                     /translation="MTQAILEIKHLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STFLRSINLLEEPSGGEILYHGHNVLEKGYDLNNYREKLGMVFQSFNLFENLNILENA
                     IVAQTTVLKRERQEAEKIAKENLNAVGMTEQYWKAKPKQLSGGQKQRVAIARALSVNP
                     EAILFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFAKEVSDRVIFMDKGI
                     IAEQGTPKQLFENPTQERTKEFLQRFLK"
     gene            complement(1642..3192)
                     /locus_tag="VBC52_001472"
     CDS             complement(1642..3192)
                     /locus_tag="VBC52_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO6883052.1"
                     /translation="MKKILLSLFTALLITFGGMTSIQADEYLRVGMEAAYAPFNWTQN
                     DNTNGAVPIEGTDQYANGYDVQVAKKLAKKLNKKVVVVKTKWEGLVPALTSGKLDMII
                     AGMSPTEERKKEINFSKPYYISEPTLVVNAEGKYTNAKNISDFKNAKVTAQQGVYLYN
                     LIDQINGVKKEVAMGDFNQLRQAVESGVVDAYVSERPDATSAQTANPKLKMIELHQGF
                     KTSDADTNISVGMRKGDNRINQVNQVLESISRDKQIALMDKMIKEQPSVKKEKNGKPN
                     FFEQMATILKNNGSQFLRGTATTLLISMVGTIVGLFIGLLIGVFRTAPKSDNKLKAAL
                     QKLLGWLLNIYIEVFRGTPMIVQSMVIYYGTAQAFGVSLDRTLAAIFIVSINTGAYMS
                     EIVRGGIFSVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFILTFTVTRILRYIEKRFDSDNYT
                     TGANQLQV"
     gene            3328..5211
                     /locus_tag="VBC52_001473"
     CDS             3328..5211
                     /locus_tag="VBC52_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEO6883053.1"
                     /translation="MKKCFLAICLALSFFMVSVQADEVDYNIPHYEGNLTIHNDNSAD
                     FTEKVTYQFDSSYNGQYVTLGTAGKLPDNFDINNKPQVEVSINGKVRKVSYQIEDLED
                     GYRLKVFNGGEAGDTVKVNVQWKLKNVLFMHKDVGELNWIPISDWDKTLEKVDFWIST
                     DKKVALSRLWGHLGYLKTPPKIRQNNNRYHLTAFNVNKRLEFHGYWDRSYFNLPTNSK
                     NNYKKKIEYQEKMIERHGFILSFLLRILLPSFFIIVTLFISIRVFLFRKKVNKYGQFP
                     KEHHLYEAPEDLSPLELTQSIYSMSFKNFQDEEKKTHLISQEQLIQSILLDLIDRKVL
                     NYDDNLLSLANLDRASDAEIDFIEFAFADSTSLKPDQLFSNYQFSYKETLRELKKQHK
                     ASDLQTQMRRRGSNALSRITRLTRLISKDNINSLRRKGISSPYRKMSSEESKELSRLK
                     RFSYLSPLISFVVIIYTLFLNYFTYFCIYLLLFGVILLLNKIIFMMTRKISNGYIVTE
                     DGASRVYQWTSFRNMLRDIKSFDRSELESIVLWNRILVYATLFGYADRVEKVLRVNQI
                     DIPERFANIDSHQFAISVNQSSNHFSTITEDVSHASNFSVNSGGSSGGFSGGGGGGGG
                     GAF"
     gene            5257..6096
                     /locus_tag="VBC52_001474"
     CDS             5257..6096
                     /locus_tag="VBC52_001474"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEO6883054.1"
                     /translation="MLIIELLKALFLGVVEGVTEWLPVSSTGHLILVQEFMKLNQSKS
                     FVEMFNIVIQLGAIMAVIVIYFKRLNPFQPGKSAREIRLTWQLWLKVVIACIPSILIA
                     LPFDNWFEAHFNFMIPIAIALIFYGFVFIWVEKRNAHLKPQVTELASMSYKTAFLIGC
                     FQVLSIVPGTSRSGATILGAIIIGTSRSVAADFTFFLAIPTMFGYSGLKAVKYFLDGN
                     VLSLDQSLILLVASLTAFVVSLYVIRFLTDYVKRHDFTIFGKYRIVLGSLLILYWLVV
                     HLF"
     gene            6216..6971
                     /gene="mecA"
                     /locus_tag="VBC52_001475"
     CDS             6216..6971
                     /gene="mecA"
                     /locus_tag="VBC52_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEO6883055.1"
                     /translation="MEMKQISETTLKITISMEDLEDRGMELKDFLIPQEKTEEFFYSV
                     MDELDLPENFKNSGMLSFRVTPKKDRIDVFVTKSELSKDLNLEELADLGDISKMSPED
                     FFKTLEQSMLEKGDTDAHAKLAEIENMMDKATQEVVEENVSEEQPEKEVETIGYVHYV
                     FDFDNIEAVVRFSQTIDFPIEASELYKNGKGYHMTILLDLENQPSYFANLMYARMLEH
                     ANVGTKTRAYLKEHSIQLIHDDAISKLQMIEMG"
     gene            6973..8133
                     /locus_tag="VBC52_001476"
     CDS             6973..8133
                     /locus_tag="VBC52_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEO6883056.1"
                     /translation="MIPFTIEYIFVLIGAFLLSIILTPIIRVISLKVGAVDKPNARRI
                     NKVPMPSSGGLAIFLSFVVTTLFFMPMAASRHFIEVSYFHYILPVIIGGLVVTTTGFI
                     DDIFELRPRYKMLGIIIAAIIIWKFTHFRFDSFKIPIGGPLLEFGPILTFFLTVLWII
                     SITNAINLIDGLDGLVSGVSIISLATMAVVSYFFLPKIDFFLTLTIVILIASIVGFFP
                     YNYHPAIIYLGDAGALFIGFMIGVLSLQGLKNSTAVAVITPVIILGVPILDTAVAIVR
                     RKLSGKKISEADKMHLHHRLLSMGFTHRGAVLVVYGIAIIFSLIALLLNVSSRIGGIF
                     LLLALLLAMEIFIEGLNIWGENRTPLFNLLKFIGNSDYRQSVIAKYSDKHQK"
     gene            8298..9068
                     /gene="sufC"
                     /locus_tag="VBC52_001477"
     CDS             8298..9068
                     /gene="sufC"
                     /locus_tag="VBC52_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEO6883057.1"
                     /translation="MSVLEIKNLHVSIEDKEILKGLNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTAGEILFDGEDILELEVDERARLGLFLAMQYPSEVPGITNAEFIR
                     AAMNAGKADDDKISIRQFITKLDEKMELLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGEGFGAMIITHYQRLLNYITPDKVHV
                     MMDGKVVLSGGPELAVRLEKEGYAQIAEELGLEYKEEV"
     gene            9105..10367
                     /gene="sufD"
                     /locus_tag="VBC52_001478"
     CDS             9105..10367
                     /gene="sufD"
                     /locus_tag="VBC52_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEO6883058.1"
                     /translation="MSKEAILNFLQAKGEPTWLQELRLKAFEKIEELELPVIERVKFH
                     RWNLGDGTILENDYTANVPDFTELGNNPKLVQIGTQTVLEQVPMELIEKGVVFTDFYS
                     ALEEIPEVIERYFGKARPFEEDRLAAYHTAYFNSGAVLYIPDNVEITQPIEGLFYQDS
                     QSKVPFNKHILLIVGKNAKVSYLERFESIGDGTERTSANISVEVIAQAGSQIKFASID
                     RLGENVTTFISRRGRHSSDATIDWALGVMNEGNVVADFDSDLIGDGSHANLKVVAASS
                     GRQVQGIDTRVTNYGCNSVGHILQHGVILERGTLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDKARSDANPILLIDENDVTAGHAASIGQVDPEDLYYLMSRGLNQKTAEQLVIRGF
                     LGTVIAEIPVKEVRDEMIAVIDTKLEKR"
     gene            10369..11601
                     /locus_tag="VBC52_001479"
     CDS             10369..11601
                     /locus_tag="VBC52_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6883059.1"
                     /translation="MTLLDSYKLKQDFPILNQLVNDEPLIYLDNAATTQKPNQVLEAL
                     RDYYQNDNANVHRGVHTLAERATAQYENAREKARQFLNAKLSKEILFTRGTTTGLNWV
                     AKFAESILERGDEVLISIMEHHSNIIPWQQACERTGAKLVYAYLKDGSLDLEDFYNKL
                     SSKTKFVSLAHISNVLGCVTPVKAIAERVHQVGAYMVVDGAQSAPHMAIDVQDLDCDF
                     FALSGHKMLGPTGIGVLYGKESILDKMPPVEFGGEMIDFVYEQSATWKELPWKFEAGT
                     PNIAGAIAFGEALDYLTDVGMDEIHQYEQSLVSYVLPKLQAIDGLTIYGPSDAESHVG
                     VIAFNLEGLHPHDVATAMDYEGVAVRAGHHCAQPLINHLGIHSAVRASFYFYNTKEDC
                     DKLVDAIQKTKEFFNGTL"
     gene            11588..12031
                     /locus_tag="VBC52_001480"
     CDS             11588..12031
                     /locus_tag="VBC52_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEO6883060.1"
                     /translation="MALSKLDNLYMAVVADHSKHPHHHGFLEGVEQVQLNNPTCGDVI
                     SLSVKFDGNIISDIAFAGNGCTISTASSSMMTDAVIGKTKEEALQLADVFSKMVQGDQ
                     NPKQEKLGDAEFLAGVSKFPQRIKCATLSWNALRKAIERDNQAEL"
     gene            12131..13549
                     /gene="sufB"
                     /locus_tag="VBC52_001481"
     CDS             12131..13549
                     /gene="sufB"
                     /locus_tag="VBC52_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEO6883061.1"
                     /translation="MSEINEKVEPQPIDLGEYKFGFHDDVKPIYSTGKGLNEAVIREL
                     SAAKGEPEWMLDFRLKSLETFNKMPMQTWGADLSDIDFDDIIYYQKASDKPARDWDDV
                     PEKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEYDKLGIVFTDTDSALKEY
                     PELFKKYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRATAKKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANKGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKDSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSEAEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(13621..14802)
                     /locus_tag="VBC52_001482"
     CDS             complement(13621..14802)
                     /locus_tag="VBC52_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO6883062.1"
                     /translation="MPKLIVSFLCILLSLTCVNSVQAEEHKDIMQITREAGYDVKDIN
                     KPKASIVIDNKGHILWEDNADLERDPASMSKMFTLYLLFEDLAKGKTSLNTTVTATET
                     DQAISKIYEISNNNIHAGVAYPIRELITMTAVPSSNVATIMIANHLSQNNPDAFIKRI
                     NETAKKLGMTKTHFYNPSGAVASAFNGLYSPKEYDNNATNVTTARDLSILTYHFLKKY
                     PDILNYTKYPEVKAMVGTPYEETFTTYNYSTPGAKFGLEGVDGLKTGSSPSAAFNALV
                     TAKRQNTRLITVVLGVGDWSDQDGEYYRHPFVNALVEKGFKDAKNISSKTPVLKAVKP
                     KKEVTKTKSIQEQPQTKEQWWTKTDQFIQSHFVSILIVLGTIAILCLLAGIVLLIKRS
                     R"
     gene            complement(14955..16190)
                     /locus_tag="VBC52_001483"
     CDS             complement(14955..16190)
                     /locus_tag="VBC52_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO6883063.1"
                     /translation="MKKIITSILLLSCIFFMPTISAESFNASAKHALAVDLDSGKILY
                     EKDANKPAAIASLTKIMTVYMVYKEIDNGNLKWNTKVNISDYPYQLTRESDASNVPLE
                     KRRYTVKQLVDAAMISSANSAAIALAEHISGTESKFVDKMTAQLEKWGIHDSHLVNAS
                     GLNNSMLGNHIYPKSSQNDENKMSARDIAIVAYHLVNEYPSILKITSKSVAKFDKDIM
                     HSYNYMLPDMPVFRPGITGLKTGTTELAGQSFIATSTESGMRLLTVIMHADKADKDKY
                     ARFTATNSLLNYITNTYEPNLVLAKGAAYKGKEASVRDGKEQSVIAVAKNDLKVVQKR
                     NITKQNQLKINFKKELTAPITKKENLGKAYYVDLNKVGKGYLIKEPSVHLVAKDSIER
                     SFFLKVWWNHFVRYVNEKL"
BASE COUNT         5153 a         2607 c         3180 g         5352 t
ORIGIN      
        1 attaaatatc aatcaaaagt aataagttca aagatatatt aaacctttat taatgaggtt
       61 tggatttgac aagtgatgtt aagtgaaaga tgaatgagga aaatacccaa cgagaaagta
      121 gctaatggct atcattgtgc atataaaaaa acgatcgatg agtgatcgtt tttcagcaaa
      181 ctaaataatt atttttaatg taattgaatg aaaatttata cttgtgaaag tgtatcggtt
      241 aagttaataa ggtcagtttt tatttatcgt aagcacttag gaagtgtttt ggagcatacc
      301 cacgagagta gataaatccg gcatcgcgtg aaggatagct tgattcagaa gacgcatacc
      361 cacgagagta gataaacccg gcatcgcgtg aaggatagct tgattcagaa ggcgcatacc
      421 cacgagagta gacaaatccg gcatcacgtg aaggataaga cactgtgtct gccttcgcaa
      481 gacttgctaa agtagctaat aatagagtga cagctgaagc tgctgttaaa acactgattt
      541 taagtttcat tataaaatcc tttcaaagat aataaactat tcttgcacag tggttagttc
      601 attttgtgca tactatatta tatcatcaaa agctcattcg aacaacatgt ttttaatttt
      661 aaacaaatat atgattaatc aaatatcgct taaaattaaa aaatgagatt tagtaactta
      721 tttaatagta tacaatccaa taacgactgt ctaatttgat ctatagttga agctagtcgt
      781 ggatttcact aaagatgtag caataaaaaa tcccctccag ttccattgca atgatgacaa
      841 tgacaaaacc tgtaggggta taaatagtct atatttaaat gtgaataaat tttattttaa
      901 gaagcgttgt agaaattcct tagttctttc ttgtgttgga ttttcgaaaa gttgtttagg
      961 agtaccctgc tctgcaatga ttcctttgtc catgaaaata acgcggtctg atacttcttt
     1021 agcaaattcc atctcgtgtg tcacaattat cattgttaat ccagatttag ctagatcttg
     1081 cattgtttta aggacctcac caaccatttc tggatccaag gctgaagttg gttcatcaaa
     1141 aagtatagct tctgggttaa cagaaagtgc tcgggcgatt gcgacacgtt gcttttgccc
     1201 acctgataat tgttttggtt tagctttcca atattgctca gtcatgccaa cagcatttaa
     1261 gttttcttta gcaatctttt cagcttcttg gcgttcacgt tttagaacag tagtctgtgc
     1321 gacaatagca ttctctaaga tatttaaatt ttcaaagagg ttaaaagatt gaaagaccat
     1381 cccaagtttt tcacgataat tatttaaatc atagcctttt tcaagaacat tgtgaccgtg
     1441 atataagatt tctcctccac ttggttcttc taagaggtta atagaacgta ggaaagttga
     1501 ttttcctgat ccagaagaac caatgataga aataacttcg cctttgttga ccgaaagcga
     1561 aatatctttt aacacttcat tgcttccgta agattttttc aaatgtttaa tttctaaaat
     1621 agcttgtgtc ataccaacct cctagacttg taattgattc gcaccggttg tgtaattatc
     1681 tgaatcaaaa cgtttttcaa tgtaacgtaa gatacgtgtt acggtgaagg ttaagatgaa
     1741 gtaaataatc gcaataatgg tgaatgtttg gaaatattgg taggtttgtg ttgcaacagt
     1801 attaccagag aagtagagtt cgacaacaga gataacgttc agtacagagg tatctttgat
     1861 attgataaca aactcattac cagtagctgg gagtatgtta cgtacaactt gtggtagaac
     1921 aatttttcgc atggtttgtc cgtgggtgaa accaagtgca gttgcggctt caaattgtcc
     1981 tttatcaaca gagaaaatac caccacgtac aatttcgctc atataagcac cagtattgat
     2041 tgaaacaata aatatagctg ctagtgtacg gtccagcgaa actccaaaag cttgtgctgt
     2101 tccgtagtag ataaccatcg attgtacaat cataggtgtc ccgcggaaga cctcaatata
     2161 gatgttaagt aaccaaccta ataatttttg taaggcagcc tttaacttat tgtcagattt
     2221 tggtgctgta cggaaaacac cgatgagaag gccgataaat aatccgacga ttgttcctac
     2281 catagaaatt aggagggttg tagctgttcc tctgaggaat tgagaaccat tgttttttaa
     2341 aatagttgcc atctgttcaa agaagtttgg tttcccattt ttttcttttt taacggatgg
     2401 ctgttcttta atcatcttat ccatcaaggc gatttgttta tctctagaaa tgctttcgag
     2461 aacttgatta acttgattaa tacgattgtc acctttacgc atcccaacag aaatatttgt
     2521 atcggcatca gatgttttaa acccttggtg cagttcaatc atttttaatt taggattagc
     2581 agtttgagcg gaagttgcat ctggacgttc tgaaacatag gcgtctacaa caccagattc
     2641 tacagcttgt cttaattgat tgaagtcccc catggcaact tcttttttga caccattgat
     2701 ttgatcaatg aggttgtata agtagactcc ttgttgggca gttactttag catttttgaa
     2761 atcactaata tttttagcat tagtatattt tccttcagca ttaacaacta gagtaggttc
     2821 tgaaatgtag taaggctttg aaaaattaat ttctttttta cgttcttcgg taggactcat
     2881 tcctgcaata atcatatcga gttttccaga agttagtgct ggaacaaggc cttcccattt
     2941 tgttttgaca acaacaactt ttttatttaa ctttttagct aactttttag cgacttgtac
     3001 atcataacca ttagcatatt gatcagttcc ttcaatagga actgcaccat ttgtgttatc
     3061 attttgtgtc cagttgaaag gagcataggc tgcctccata ccgacacgta gatattcatc
     3121 tgcttgtata cttgtcatac ctccaaaggt gataagcaaa gcagtgaata aacttagtaa
     3181 tatttttttc attcaacagc ctccgataag ttacttcctc ctattttacc gaaaaacaag
     3241 gtcgatttca atatcattat cttattgaaa gcgattaatt gtatttttat gttaaaatgg
     3301 tatacaaaca aaggaaggag gatgataatg aagaaatgtt ttttggctat ttgtttagct
     3361 cttagttttt ttatggtttc agttcaagca gatgaggtgg actataacat tcctcattat
     3421 gagggtaatc taactattca caatgataat agtgctgatt ttacagagaa ggttacttac
     3481 caatttgatt cgtcctataa tggacagtat gtcacattag gtacggcggg taagttacct
     3541 gacaattttg atattaataa taagccacag gttgaagttt caattaatgg taaagtaagg
     3601 aaagttagtt accagataga agatttggag gatggctacc gtttgaaagt gtttaatggt
     3661 ggtgaagcag gtgatactgt taaagtcaat gttcagtgga aactaaaaaa tgttctattt
     3721 atgcataagg atgttggtga acttaactgg attcctatta gcgactggga taaaacgtta
     3781 gagaaagtag atttttggat atcaactgac aaaaaggttg ctctttctcg tctttggggg
     3841 cacttgggtt atcttaaaac tcctcctaaa ataagacaaa ataataatcg ttaccatttg
     3901 acagctttta atgtaaacaa acgattagaa tttcatggtt attgggatag atcttatttt
     3961 aatctaccta caaacagtaa aaataattac aagaaaaaaa ttgaatatca agagaagatg
     4021 atagagcgtc atggttttat cctaagtttc ttgttaagga tattattacc ttcattcttt
     4081 attattgtga cactattcat ctcaattagg gtgttcctgt ttagaaaaaa agttaataaa
     4141 tacgggcaat tccctaagga acatcattta tatgaagcac ctgaggacct ttcaccacta
     4201 gagttaactc aaagcattta tagtatgagt tttaaaaatt ttcaagatga ggagaagaaa
     4261 actcacctta tcagtcaaga acaactcatt cagtcaattc tattagactt gattgataga
     4321 aaagtattga attatgatga taacttgtta tctctagcta acttagatag agcttctgat
     4381 gcagaaatag attttataga gtttgctttt gcggattcta cgagtttgaa gccagatcaa
     4441 ctcttttcta attaccaatt tagttataaa gaaacactac gtgaactgaa aaagcagcac
     4501 aaggcttcag atctgcaaac tcaaatgaga cgccgaggaa gtaatgcctt atcaagaatt
     4561 acgcgtctca caaggttgat ttctaaagac aatataaact ctcttagaag aaagggaatt
     4621 tcatcccctt atcgtaaaat gtcttcagaa gagtctaaag aattatctag gttgaaaaga
     4681 ttcagttacc tatcacctct tatttctttt gttgttataa tttatacgct ttttttaaat
     4741 tattttacct atttctgtat ctatctctta ttgtttggtg ttatcctgtt gttgaataaa
     4801 atcattttta tgatgacaag aaaaataagt aacggttata ttgtaactga agatggagca
     4861 agtcgtgtct accaatggac tagttttagg aacatgctaa gggatatcaa atcgtttgat
     4921 cgttcagagt tagaaagtat cgtattatgg aatcgaatat tggtttacgc tactttattc
     4981 ggctacgctg accgtgttga gaaagtactc agagtgaacc aaatagatat tccagaaaga
     5041 tttgcaaaca ttgatagtca tcaatttgcg atttcagtca atcaatctag taatcatttt
     5101 tcaacgataa ctgaagatgt tagtcacgct tctaatttta gtgttaattc aggcggttct
     5161 tcaggtggtt tctcaggcgg cggaggcggc ggaggtggtg gtgccttcta attttccaaa
     5221 agtatggtat aataaagaaa aatttatgga gttattatgt taataattga attactcaaa
     5281 gccctatttt taggagtcgt tgagggagtg acagaatggt tacctgtttc tagtactggc
     5341 catttaatct tggttcaaga gtttatgaag ttgaaccaaa gcaaatcctt tgttgaaatg
     5401 tttaatattg ttatacaact tggggcaatt atggcagtta ttgtcattta ttttaaacgc
     5461 cttaacccat ttcagccagg taaatctgct agagaaattc gtttaacttg gcaattatgg
     5521 ttaaaagtag tgatagcttg tattccatct attttaattg ctttaccatt tgataattgg
     5581 tttgaagctc attttaattt catgatcccg attgcaatag ccctaatctt ttatggtttt
     5641 gtcttcatat gggttgaaaa acgtaatgca cacctcaaac cacaggtaac cgaattggca
     5701 agtatgtctt acaagacagc tttcttgatt ggatgcttcc aggttctcag tattgttccg
     5761 ggaaccagtc gttctggagc tactatttta ggagcaatta ttattggaac tagtcgttcg
     5821 gtcgctgctg actttacttt cttccttgcc atcccaacta tgtttggtta tagtggactt
     5881 aaggcggtta aatacttttt agatggtaac gtcttgagtt tagaccaatc tttaatactt
     5941 ttagtagcaa gtctgacagc tttcgtagtt agtttatatg ttattcgttt cttgacagac
     6001 tatgtcaaac gacacgattt caccatcttt ggtaagtatc gtatagtctt aggaagttta
     6061 ctcatcctct actggttagt tgttcattta ttctaaaata taacataaaa agctgagtat
     6121 tcctcagctt tttagcgtgt taggctgtga aaaattgcta aaaaatagct ttttcggtat
     6181 aataagaaaa ctgaatactc gtatgaggtg atgatatgga aatgaaacaa atcagtgaaa
     6241 caacactgaa aattacaatt agtatggaag atttagaaga tcgtggtatg gagctgaaag
     6301 atttcctaat ccctcaggag aagactgagg aatttttcta ttctgtcatg gatgaattag
     6361 acttgccaga aaactttaaa aatagtggta tgttaagttt tcgagtaaca cctaaaaaag
     6421 atcgcattga tgtttttgtt acaaagtctg aattaagtaa agatttaaat ttagaagaat
     6481 tagcagattt gggtgacatt tcaaaaatgt ctccagaaga cttttttaaa acattggaac
     6541 aatcgatgtt ggaaaaaggg gatacggatg cccatgccaa attagcagaa attgaaaata
     6601 tgatggataa agcaactcaa gaagtagttg aggaaaatgt ttctgaagaa caacctgaaa
     6661 aggaagtaga aacgattgga tatgttcact atgtctttga ttttgataat attgaagctg
     6721 tagttcgatt ttcacaaacg attgattttc caatagaagc ttctgaactc tataaaaatg
     6781 gtaaaggcta tcatatgaca attttgctag acttggaaaa ccaaccaagc tactttgcta
     6841 atttgatgta tgctcgaatg cttgaacatg ctaatgtggg cacaaaaaca cgagcttact
     6901 tgaaagagca tagtattcaa ttgattcatg atgatgctat ttcaaaatta caaatgattg
     6961 agatgggata aaatgatacc ttttacaatt gagtatattt ttgtattgat tggagcattt
     7021 ttattatcaa tcattctaac tcctattatt cgagttatat ctttgaaggt aggagcggtt
     7081 gataaaccga acgctcgaag aatcaacaaa gtaccaatgc caagtagtgg tggtttagca
     7141 atatttctct cctttgttgt aacaacatta tttttcatgc caatggcggc cagtagacac
     7201 tttatagaag tctcttattt tcactacatt ttgcctgtaa ttattggcgg tctcgtagtt
     7261 acaacaacag gattcataga tgacatcttt gaattaagac ctcgttataa aatgttggga
     7321 attattattg cagctattat catatggaaa tttacacatt ttagatttga tagttttaag
     7381 attccaatag gtggtccact cttggagttt ggtcctattt taactttctt tttgacagtt
     7441 ttatggatta tttcaatcac taatgctatc aatctgattg atggtctaga tggtctagtt
     7501 agtggtgttt caattatcag tctagcaacg atggcagtgg tttcttattt ctttttacct
     7561 aaaatagatt tttttctcac gctaactatt gttattttaa tcgcctcaat agttggtttt
     7621 ttcccttata attatcatcc agccattatc tacctaggtg atgctggtgc cttgtttata
     7681 ggatttatga ttggtgtatt atcgcttcaa ggtttaaaaa actccacagc tgttgcagtg
     7741 ataacacctg tcattatttt aggagttcct attttggata cagctgttgc tattgtgagg
     7801 cgtaaattat cagggaaaaa gatttctgaa gctgataaga tgcacctgca tcaccgattg
     7861 ctatctatgg gatttacaca tagaggagca gttttagttg tttatggtat tgcaattatc
     7921 ttttcgctta tcgctttgct tttaaatgtt tctagtcgta ttgggggaat ttttcttcta
     7981 ttggcgctac tattagcgat ggaaattttt atagaaggtt taaatatttg gggtgagaat
     8041 cggacaccac tatttaatct cttaaagttc attggcaaca gtgattacag acaaagtgtt
     8101 attgctaaat atagcgataa gcatcaaaaa taaaagcctg aaaaggcttt tttagtttta
     8161 atcatttaga tagcagtcac acctcatttt tgctataatt atttagaata taatatcggg
     8221 tgagtgccct ataatatctt ctttaaacca tagagaaata tcacatattc tctgttaacc
     8281 aagaaaagga gtgtacaatg tctgtacttg aaatcaaaaa tcttcatgtt tctattgaag
     8341 ataaagaaat tttaaaaggt cttaatttaa cgttgaaaac aggagaaatt gctgctatca
     8401 tgggtccaaa tggtactggg aagtcgacac tttcagcagc tattatggga aatcctaatt
     8461 atgaagttac agcaggtgaa attctttttg atggtgaaga tattttagag ctagaagtgg
     8521 atgaacgtgc tcgtttgggt ctatttcttg ccatgcaata cccttcagag gttccaggga
     8581 ttactaatgc tgaatttatc cgagcagcta tgaatgcggg aaaggctgat gatgataaaa
     8641 tttccatccg ccaatttatc actaagttag atgaaaagat ggaactactt ggcatgaaag
     8701 aagaaatggc ggaacgttat cttaacgaag gtttttcagg tggtgagaag aagcgcaatg
     8761 aaattttaca actcctaatg ttagagccta agtttgccct cttggatgag attgactctg
     8821 gccttgatat tgatgctctt aaagtggtat caaagggagt caatgaaatg cgtggtgaag
     8881 gttttggtgc tatgattatt acacactatc aacgcctttt gaattacatt acaccagata
     8941 aagtacacgt tatgatggac gggaaggtag tattgtcagg aggaccagaa ttagcggttc
     9001 gccttgagaa agaaggttat gctcaaatcg ctgaagagct tggtttagag tataaagaag
     9061 aagtctaatt tgtcacttca actagaagag aaaaggagtt acaaatgtct aaagaagcta
     9121 tattaaattt tttacaagcc aaaggtgagc ctacttggtt acaagaatta cgactaaagg
     9181 cttttgaaaa aatagaagaa ctagaattac cagtaattga acgtgtaaaa tttcatagat
     9241 ggaatttagg tgatggaacc attttagaga atgactatac agctaacgtt ccagatttca
     9301 cagagttagg aaacaatccc aagttggttc aaattgggac ccaaaccgtc cttgaacaag
     9361 ttcctatgga attaatagag aaaggcgttg tctttacaga tttctattcg gccttggaag
     9421 aaattccaga agtcatagag cgctactttg ggaaagcacg tccttttgaa gaagatagac
     9481 ttgctgccta tcacacagct tacttcaaca gtggtgcagt gctttatatt ccagataatg
     9541 ttgaaattac tcaaccaatc gaaggtcttt tttatcaaga tagtcagtct aaagtaccat
     9601 ttaataagca tatccttctt attgttggaa agaatgcaaa agttagttac ttagagcgat
     9661 ttgagtcaat tggtgatggg acagaacgaa caagtgctaa tatttctgta gaagtaattg
     9721 cccaagcagg tagccaaatt aaatttgctt caattgatcg cctcggagaa aatgtgacaa
     9781 cgtttataag tcgtcgtggt cgtcacagta gcgatgcaac aatagattgg gcccttgggg
     9841 tgatgaatga aggcaatgtt gttgctgact ttgattctga tttaattggt gatggaagtc
     9901 acgctaattt aaaagtagtt gctgcctcgt cagggcgtca agttcaagga attgatacgc
     9961 gtgttacaaa ctacggctgt aattcagtag gacatatttt acagcatggg gttattttag
    10021 agcgtgggac tcttactttt aatggtatcg gacatattat aaaaggtgca aagggagcag
    10081 acgcccaaca agaaagccga gttctcatgt tatcagacaa agcacgttca gatgctaatc
    10141 ctatcctttt gattgatgaa aatgatgtta cagctggtca cgcagcctca attggtcagg
    10201 ttgacccaga ggatttatat tatttgatga gccgaggctt gaaccaaaaa acagcagagc
    10261 aattagttat tcgtggattt ttaggaacag tcattgctga aataccagta aaagaagttc
    10321 gcgatgaaat gattgccgtt attgatacaa aactagaaaa acgttaatat gacattacta
    10381 gatagttata aactaaagca agatttccca attttaaatc aattagttaa tgatgagccg
    10441 cttatctatt tagataatgc agcaacgaca caaaaaccga atcaagtttt agaagctttg
    10501 cgagactact accaaaacga caatgcaaat gtccatcgtg gtgttcatac tttagcagaa
    10561 cgagcgactg ctcagtacga gaatgctcgt gaaaaagcaa ggcagttttt aaatgctaaa
    10621 ttaagtaaag aaattttatt cacacgtggc accacaacag gattaaattg ggtagctaag
    10681 tttgccgaaa gtattttaga acgaggtgat gaggtactta tttccattat ggagcatcat
    10741 tctaatatta tcccatggca acaagcttgc gagagaactg gcgctaaatt ggtttatgcc
    10801 tatctaaaag atggttcttt agatttagaa gatttctata ataaattatc ctctaaaact
    10861 aaatttgtta gtttggcaca tatctcaaat gtacttggct gtgttacacc agtaaaagca
    10921 atagcagaac gtgtgcatca agttggggca tacatggttg ttgatggagc tcaatcagcg
    10981 ccacatatgg cgattgatgt tcaagacctt gattgtgatt tcttcgcctt atctggccat
    11041 aagatgcttg gtcctactgg tatcggtgtt ctttatggga aagaaagcat tttagataag
    11101 atgccacctg tcgaatttgg tggtgaaatg attgattttg tctatgagca aagtgcaaca
    11161 tggaaggaat tgccctggaa gtttgaagct ggaacgccta atattgctgg agcgatcgct
    11221 tttggagaag ctttagatta tttaacggat gttggtatgg atgagattca tcaatatgaa
    11281 caaagtttag tttcttatgt gttgcctaaa ttacaggcta tcgatggttt aactatttac
    11341 ggacctagtg atgctgaatc tcatgtaggc gttattgcct tcaatttaga aggtttacac
    11401 ccacatgatg tggcaactgc aatggattac gaaggagtgg ctgttcgtgc aggtcatcat
    11461 tgtgcacaac cccttattaa tcatcttggt atccattcgg cggttcgagc aagtttctat
    11521 ttctataata ccaaagagga ttgcgacaaa ttggtagacg ctatccaaaa aactaaggag
    11581 tttttcaatg gcactctcta aattagataa tctctacatg gcagtggtag ctgatcattc
    11641 aaaacaccct catcatcatg gatttttaga aggcgttgaa caagtgcagt taaataatcc
    11701 tacatgtgga gatgttattt ccttatctgt taaatttgat ggaaatatta tttcagatat
    11761 tgcatttgct ggtaatggtt gtacaatttc tacggcttca tcaagcatga tgacagatgc
    11821 agttattggg aaaacaaaag aagaagcatt gcagcttgct gatgtattct ccaaaatggt
    11881 tcaaggcgat caaaatccaa aacaagagaa acttggggat gcagagtttc tagctggggt
    11941 atcaaaattt ccacaacgta tcaaatgtgc tacgctttcg tggaatgctt taagaaaggc
    12001 tattgagaga gataatcagg cagaactctg acaaccatag ttgaaggaat tacatttaaa
    12061 caacttttac tgacataagg tagtctagta gaagaatgtc ttaaatttaa atattaaaga
    12121 aaggataagt atgtccgaaa taaatgaaaa agtagaacca cagccaatag atcttgggga
    12181 atataaattt ggatttcatg acgatgtaaa gccaatttat tctacgggaa aaggtctaaa
    12241 tgaggctgtt attcgtgagt tatctgcagc taagggtgaa cctgagtgga tgttggactt
    12301 tcgtctaaaa tccttggaaa cgtttaataa aatgccaatg cagacctggg gagcagattt
    12361 atcagatatt gattttgatg atattattta ttatcaaaaa gcatctgata aacctgcgcg
    12421 tgattgggat gatgttccag aaaaaatcaa agaaactttt gaaagaattg ggattccaga
    12481 agctgaaaga gcttatcttg caggagcatc agcacaatat gaatcagaag tggtttatca
    12541 taatatgaaa gaagaatatg ataagctggg tattgttttt acggatactg actctgcact
    12601 taaagagtac ccagagctat tcaaaaaata ttttgctaaa cttgtccctc caacagataa
    12661 taaattagct gctctgaact ctgctgtatg gtcaggtgga acatttattt atgtccctaa
    12721 aggtgttaag gtggatattc cacttcaaac ttacttccgt attaataatg aaaatactgg
    12781 acaatttgaa cgtactctca ttattgtcga tgagggagca agtgttcact atgttgaagg
    12841 ttgtaccgcc ccaacttatt cttcaaatag tttacatgca gctatagttg aaatttttgc
    12901 acttgatgga gcttatatgc gctatacgac tattcaaaat tggtccgata atgtctataa
    12961 tttagtgaca aaacgtgcta ccgctaaaaa agatgcaaca gttgagtgga tagatggaaa
    13021 tctaggtgct aaaacaacaa tgaaataccc atcggtttac cttgatggtg aaggagcacg
    13081 tggcacgatg ttgtctattg cttttgcaaa caaaggacaa caccaagata cgggtgcaaa
    13141 gatgattcat aatgctcccc atactagttc atccattgtc tctaaatcaa ttgctaaggg
    13201 tggaggaaaa gttgattatc gaggtcaagt gacatttaat aaagattcca aaaaatcagt
    13261 gtcacatata gaatgtgaca ccatattgat ggatgatatt tcaaaatcag ataccatacc
    13321 gtttaatgag attcataatt cacaggttgc tttagagcat gaagcaaagg tgtctaagat
    13381 ttctgaagag caactgtact acttgatgag tcgaggttta tctgaagctg aggcaacaga
    13441 aatgattgtt atggggtttg ttgagccctt tacgaaagaa ttaccaatgg aatatgcggt
    13501 agagttaaac cgcttaattt cctatgaaat ggaaggttca gttggttaat tgaagatttc
    13561 aagagataat aaaaagcccc tatgttattg atgaaggggg cttttatatt agcacagtta
    13621 ttatctagag cgctttataa gtaatactat cccagctaaa agacaaagga tagcgatggt
    13681 gcccagaaca attaaaatag atacaaaatg tgattgaata aattgatctg tttttgtcca
    13741 ccactgttct tttgtttgag gctgttcttg aatagatttg gttttagtaa cttctttttt
    13801 aggtttaacg gcttttaata caggagtttt agaagaaata tttttagcgt ctttaaaacc
    13861 tttttctaca agagcgttga caaacggatg acgatagtac tctccgtctt ggtctgacca
    13921 atcaccaact cctaaaacca cagttatcaa gcgagtattc tggcgtttag ctgtaactaa
    13981 ggcattaaaa gcagcgctag ggctagaacc agtttttaag ccatctactc cttctaatcc
    14041 aaatttagcg ccgggggtag agtagttata agttgtaaat gtttcttcat aaggagttcc
    14101 gaccatggcc ttgacttcag gatattttgt atagttcagt atatcagggt attttttaag
    14161 gaaatgatag gttaaaattg atagatcacg tgcagtcgta acgttagtag cattgttatc
    14221 gtattctttt ggggagtaaa gtccattaaa agcactcgct accgccccac tggggttata
    14281 aaagtgagtt tttgtcatac cgagtttctt ggcggtttca ttgattcgtt taatgaaggc
    14341 gtcaggattg ttttgtgata agtggttagc aatcataata gttgctacat tagatgacgg
    14401 gacagctgtc atagtaatca gttcacgaat aggataagca accccagcat gaatattatt
    14461 attactaatt tcataaatct tacttatggc ttggtctgtt tctgttgcag tcactgtggt
    14521 gttgaggctt gtttttcctt tagctaagtc ttcaaatagt aaatataaag taaacatttt
    14581 agacatgcta gcgggatcac gttctaaatc agcgttatct tcccacaaaa tatgaccttt
    14641 attgtcaata acgatagacg ctttaggttt attaatatct ttaacatcat atccggcttc
    14701 tcgggtaatt tgcataatat ctttatgttc ttcagcttgc acagagttta cacaagtcag
    14761 ggataataaa atgcagagga aagatacgat taatttaggc ataggtaact cctgaaaact
    14821 ttttattaat attataacaa aaaatcacta ataaaaaaga catttcatta gtgattttta
    14881 attttatata ttctcctaaa cctagctaaa gaaactgctg tgcagcaact ccttgtgtca
    14941 aagggagatt actattaaag tttttcgtta acgtagcgca caaaatgatt ccaccacact
    15001 ttgaggaaga aactgcgctc aatactatct tttgccacta aatgaacgct aggttccttt
    15061 atgagatagc cttttccaac cttattaagg tcaacgtaat aagctttccc taggttctct
    15121 ttttttgtaa taggagcagt aagctctttt ttaaagttaa tttttaactg attttgttta
    15181 gtgatatttc tcttctgtac tactttcaaa tcgtttttag caacagcgat gaccgattgt
    15241 tcttttccgt ctctcacact tgcttcttta cctttatatg cagctccttt agctaataca
    15301 aggttaggtt cgtaggtgtt tgtgatatag ttcaagagag agttagttgc tgtaaagcga
    15361 gcatatttgt ctttatcggc cttatcagca tgcataataa cagtgagtag tctcattcca
    15421 ctttcagtag atgtagcaat aaaagattgg ccagctaatt ccgttgtccc agttttcaaa
    15481 cctgtaatac ctggtctaaa gacaggcata tctggtagca tgtagttata agaatgcata
    15541 atatctttat caaatttagc aacggactta ctagtaatct taagaatgga aggatattcg
    15601 ttgaccaaat ggtaggcaac aatagcaata tcacgtgcac tcattttatt ttcgtcgttt
    15661 tgtgacgatt ttggataaat gtgattgcct aacatactat tatttaagcc agaagcattg
    15721 actaggtggc tatcatgaat tccccacttt tccaattgag cagtcatttt atcaacaaat
    15781 ttactttcgg ttcctgaaat atgttcagcc aaagcaatgg ctgcactgtt agcactagaa
    15841 atcatggcag cgtccacgag ctgtttaaca gtatagcgcc ttttttctaa aggaacatta
    15901 ctagcatcag attcgcgtgt tagttgataa gggtagtcag atatatttac tttggtattc
    15961 cacttgaggt taccgttatc aatttcttta tagaccatat aaacggtcat tattttagtc
    16021 aaggaagcaa tagcagcggg tttgttagca tctttttcat acaagatttt tcctgaatct
    16081 aaatcaactg ccaaggcatg tttagcggaa gcattaaaag attcagcaga gatggttggc
    16141 ataaaaaaaa tgcaactaag taatagaata gaagtaataa tttttttcaa aataacacct
    16201 ttagaaattt tttaaacttt gtccctccta ttatatcata tcactatatc atactaggaa
    16261 ttattatttt tgataattaa ataaagaaaa cg
//
LOCUS       DATRNK010000048        15172 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.048, whole genome shotgun sequence.
ACCESSION   DATRNK010000048 DATRNK010000000
VERSION     DATRNK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15172
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.048"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            3..302
                     /locus_tag="VBC52_001484"
     CDS             3..302
                     /locus_tag="VBC52_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEO6883064.1"
                     /translation="MYLETLATQSNMTGKIFSMSKEELSYLPVIKLFKNQGIYNGLIG
                     LFLLYGLYISQNQEIVAIFLINVLLVAVYGALTVDKKILLKQGGLPILALLTFLF"
     gene            436..1179
                     /locus_tag="VBC52_001485"
     CDS             436..1179
                     /locus_tag="VBC52_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6883065.1"
                     /translation="MTENWLHTKDGSDIYYRVVGQGQPIVFLHGNSLSSRYFDKQIAY
                     FSKYYQVIVMDSRGHGKSHAKLNTISFRQIAVDLKDILVHLEIDKVILVGHSDGANLA
                     LVFQTMFPGMVRGLLLNSGNLTIHGQRWWDILLVRIAYKFLHYLGKLFPYMRQKAQVI
                     SLMLEDLKISPADLQHVSTPVMVLVGNKDIIKLNHSKKLASYFPRGEFYSLVGFGHHI
                     IKQDSHVFNIIAKKFINDTLKGEIVEKAN"
     gene            1163..3703
                     /gene="mprF"
                     /locus_tag="VBC52_001486"
     CDS             1163..3703
                     /gene="mprF"
                     /locus_tag="VBC52_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEO6883066.1"
                     /translation="MKKLIEKVKSLTSVIKIVFFISVLVLIIVEMIHLKRTISVEQLK
                     SVFGQLSPMNLFLIILVGVIAVLPTTGYDFVLNGLLRTDKSKRYILQTSWCINTFNNL
                     SGFGGLIDIGLRMAFYGKKGQEKSDLREVTRFLPYLISGLSFISVIALIMSHIFHAKA
                     SVDYYYLVLIGASMYFPVIYWISGHKGSHYFGDMPSSTRIKLGVVSFFEWGCAAAAFI
                     IIGYLMGIHLPVYKILPLFCIGCAVGIVSLIPGGLGSFELVLFTGFAAEGLPKETVVA
                     WLLLYRLAYYIIPFFAGIYFFIHYLGSQINQRYENVPKELVSTVLQTMVSHLMRILGA
                     FLIFSTAFFENITYIMWLQKLGLDPLQEQMLWQFPGLLLGVCFILLARTIDQKVKNAF
                     PIAIIWIILTLFYLNLGHISWRLSFWFILLLLGLLVIKPTLYKKQFIYSWEERIKDGV
                     IIVSLMGVLFYIAGLLFPIRAHITGGSIERLHYIIAWEPIALATLILTLVYLCLVKIL
                     QGKSCQIGDVFNVDRYKKLLQAYGGSSDSGLAFLNDKRLYWYQKNGEDCVAFQFVIVN
                     NKCLIMGEPAGDDTYIREAIESFIDDADKLDYDLVFYSIGQKLTLLLHEYGFDFMKVG
                     EDALVNLETFTLKGNKYKPFRNALNRVEKDGFYFEVVQSPHSQELLNSLEEISNTWLE
                     GRPEKGFSLGYFNKDYFQQAPIALVKNAEHEVVAFANIMPNYEKSIISIDLMRHDKQK
                     IPNGVMDFLFLSLFSYYQEKGYHYFDLGMAPLSGVGRVETSFAKERMAYLVYHFGSHF
                     YSFNGLHKYKKKFTPLWSERYISCSRSSWLICAICALLMEDSKIKIVK"
     gene            complement(3782..5296)
                     /locus_tag="VBC52_001487"
     CDS             complement(3782..5296)
                     /locus_tag="VBC52_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019319700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEO6883067.1"
                     /translation="MTTLITTFQERFGDWTQSLIEHLQLSLLTLILATLIAIPLGIII
                     SHYKKISHVVLQITGIFQTIPSLALLGLFIPFMGIGTVPAVVALIIYALFPILQNTVT
                     ALMQIDANLIEAATAFGMTRWERLKKFELALSMPVIISGIRTASVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNPSLILIGAISSAVLAIIFSGLIGLLEKARLRTIAVSGILLLA
                     GLGLSYAPKWMPGTNTATITVAGKLGTEPDILINMYKELIEDQTDIKVKLKPNFGKTT
                     FLYQALKSGDIDLYPEFTGTITSSLLKNPPKVSNNPKQVYNLAKNGILKQDKLSLLSP
                     MAYQNTYAVAVKKDYAEANQLKNISDLKKLDKLKAGFTLEFKDREDGSIGLQKHYGLN
                     LDISTLEPALRYQAINSKDVNIIDAYSTDSELIQYQLQILKDDKHLFPPYQGAPLLRQ
                     DTIKKYPQVKKALNKLAGHITEKEMQEMNYQVAVKHKSAATVAKQYLKAHHIIK"
     gene            complement(5299..6021)
                     /locus_tag="VBC52_001488"
     CDS             complement(5299..6021)
                     /locus_tag="VBC52_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6883068.1"
                     /translation="MISFENVSKSYGDHTIIDNISCHIQRGEFFVLVGASGSGKTTIL
                     KMINRLIEPSQGAITLDGENITSLDLRQLRLETGYVLQQIALFPNLTVGENIELIPEM
                     KGWSKGDQKKAASDLLDKVGLPAKDYFNRYPHELSGGEQQRIGILRAIVAKPKVLLMD
                     EPFSALDPISRRQLQDITKQLQSELGITLVFVTHDMKEAMRLADRICVIKEGKIVQLD
                     RPEIIQNNPSDQFVRTLFEEEN"
     gene            complement(6243..7076)
                     /locus_tag="VBC52_001489"
     CDS             complement(6243..7076)
                     /locus_tag="VBC52_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEO6883069.1"
                     /translation="MKFYIIDDDPTITMILQDIIEEDFNNTVVRVNNVSSKAYNELLI
                     ADVDIVLIDLLMPILDGVTLVQKIYKQRSDLKFIMISQVKDNDLRQEAYKAGIEFFIN
                     KPINIIEVKSVVKRVTDTIEMQKKLNTIQNLLENTPSYQKPITTSNLTKIRSILSYLG
                     ITSETAYTDILNICELLLKQELNFAQFDFQKELSIDEHQQKIILQRIRRAVKKAMINM
                     AHLYIDDFENELTLQYANALFGFQNIHNEAQLIQGKSMYGGKISLKRFFDELILQSKT
                     F"
     gene            complement(7073..8368)
                     /locus_tag="VBC52_001490"
     CDS             complement(7073..8368)
                     /locus_tag="VBC52_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO6883070.1"
                     /translation="MLDILSEKHLERKQRIIISAIVIALAAQINISILADGFIMTLSL
                     FILPVFLYFNDDINPILLCLGITFASPIFRGIILSIAGEAEIHQIIEFVLTDMAFYIC
                     YGITFYTIYWHRSYRNKGTFFFSIIICDYFANLVEISFLIKFNNYTITIFATLFAIAL
                     LRAFISCAVAYTYSYLSLLLQKDDHERRYYYFMWSTSAVKSEVYFMQKNIIEIENIMK
                     NAYLLDKELSKYHLPKEYQHLSLDISRDVHEVKKDYQNIIKGLGTYFSVKNESTMALK
                     DIFQIVLSYTRSIIQFRHQDIIILENNKCNLIISNYYYLLTIISNIVLNAVEAIDKQK
                     KGTISVHTEELEDFIKIEISDNGPGIPDKMKHMIFKPGFSTKFDANGDIYRGIGLSHV
                     RILMEEQYQGTITVCPNQPNGTTFTLLFRKNRFKLGDNT"
     gene            8471..9469
                     /gene="argF"
                     /locus_tag="VBC52_001491"
     CDS             8471..9469
                     /gene="argF"
                     /locus_tag="VBC52_001491"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO6883071.1"
                     /translation="MKNLRNRSFLTLLDFSTAEVEFLLKLSEDLKRAKYAGIEQQKLV
                     GKNIALIFEKDSTRTRCAFEVAAHDQGAHVTYLGPTGSQMGKKETSKDTARVLGGMYD
                     GIEYRGFSQETVETLAEFSGVPVWNGLTDADHPTQVLADFLTAKECLHKPYKDIRFTY
                     VGDGRNNVANALMIGASIVGMTYHLVCPKELEPDPELLSKCQEIAKTTGASIEITDDI
                     AEGVRDSDVLYTDVWVSMGEPDEVWKERIALLEPYRITQEMLNMTENPNVIFEHCLPS
                     FHNIDTKVGYDIYEKYGLKEMEVSDEVFEGPHSVVFQEAENRMHTIKAVMVATLGD"
     gene            9481..10407
                     /gene="arcC"
                     /locus_tag="VBC52_001492"
     CDS             9481..10407
                     /gene="arcC"
                     /locus_tag="VBC52_001492"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO6883072.1"
                     /translation="MAKIVVALGGNALGNSPEEQLRLVKHTAKSLVALIKKGHEIVVS
                     HGNGPQVGAINLGMNFAAESGQGTNFPFPECGAMSQGYIGYHLQQSLLNELRQEGINK
                     EVATIITQIEVDESDQAFSAPTKPIGTFYDKETSEKIAIEKGYTFVEDAGRGYRRVVA
                     SPEPKKIIEINSIKTLIENDTLVIAGGGGGIPVINKGGYEGIAAVIDKDKSSALLAGE
                     LAADQLIILTAVDYVYTQFGKENQKALTEVNENQMIDYVNQGEFAKGSMLPKVVACMS
                     FLDHNPKGTALITSLNGLEDALDGKLGTRITK"
     gene            10501..12054
                     /locus_tag="VBC52_001493"
     CDS             10501..12054
                     /locus_tag="VBC52_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEO6883073.1"
                     /translation="MPEKMTNSENKKSKGFRMPGAFTILFILTIFSVLATWWIPAGSY
                     SKLQFDTASSKLVVTDPNGKTVHVPATQTQLDKMNVKIKIKEFTSGAISKPVSVPNTY
                     KRLKQNPAGIGSVTTSMVNGTIEAVDIMVFIMVLGGMIGVVRKSGAFESGLLALTKKT
                     KGREFLLIFLVSLLMVLGGTLCGIEEEAVAFYPILVPIFLAMGYDSIICVGAIFLASS
                     VGTSFSTINPFSSVIASNAAGISFTEGLSWRTVGCTAGAIFVVVYLHWYSKKIKANPE
                     FSYSYEDRVEFNAKWGMTTNHTPSLFTVRQKIILSLFVISFPLMVWGVMSQGWWFPTM
                     ASSFLAITIIIMFLTATGANGIGERDVVDEFVNGASSLVGVSLIIGLARGINIILNQG
                     YISDTMLYTASKLASHVSGPVFIIVMMFIYFVLGFVVPSSSGLAVLSMPILAPLADTV
                     GIPRSVVVMAYQFGQYAMLFLAPTGLVMATLQMLDMKYSHWLKFVWPVVLFLLIFGGG
                     LLVLQVLIL"
     gene            complement(12162..13526)
                     /locus_tag="VBC52_001494"
     CDS             complement(12162..13526)
                     /locus_tag="VBC52_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6883074.1"
                     /translation="MLFQSKINYPKKSSIITQITLWYSSFIFILVIGVLIGSFFISKS
                     IAENKSEKNLEAKAVQMSQALAKGHRYEAFEDGIFYSVYDQNGKVIYSGFPKGFKKDL
                     DHQHKHKKKLSLFSMENRTFQYVDIPISGKNQWLRAIRTVDRLDKQLTELLFSLGIVL
                     PLILIIITVGGYLILKRAFRPIQEITETAQFITQNEDYTKRIITKNNENELTELAAVI
                     NTMLASIESSFVREKQFNNDVSHELRTPVTVILSESEYGKNYAENLIEAKESFEVIHR
                     QSLSMKKLVEQLLELTKAENPLSIQLEPLNFSIMMKQLVSDSSRLLDNTPIHLDSQIE
                     DDLWIIGQQTLLKRLFDNLFSNAIKFTNNHISISLRQSDNQIVFSIKDNGLGISVDDQ
                     SKIWNRFYQVDSARTKDSQSGIGLGLSLVKQIATIHRAKIWVDSKPDDGSQFTLTFPK
                     LKKD"
     gene            complement(13510..14181)
                     /locus_tag="VBC52_001495"
     CDS             complement(13510..14181)
                     /locus_tag="VBC52_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6883075.1"
                     /translation="MKILVVEDEFDLNRSIVKLLKKQHYSVDSASNGEEALQFVSVAE
                     YDVIILDVMMPKMDGFTFLKLLRNKGSQVSILMLTARDAVEDRIAGLDFGADDYLVKP
                     FEFGELMARIRAMLRRTNRQVSSDDIQIQDITINLSTKQVWRNNNLIDLTAKEYEVLE
                     YLARHRDQVLSRHQIREHVWDYDYDGESNIIDVLIKNLRRKLDNNRDGSLIKTKRGLG
                     YVIPK"
     gene            complement(14266..14937)
                     /locus_tag="VBC52_001496"
     CDS             complement(14266..14937)
                     /locus_tag="VBC52_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO6883076.1"
                     /translation="MTNYKNNLKKFMSNKLYVGLASITLLGIGGAIGVSGAALAHNHH
                     SEHMSFEHKHEGHDKHHDHREKHSLKTLKNISTKIDYKTALETAKKEARSGFISQIEL
                     NTKGDTPFYEIKVIDNKIAKKYKIDANSGKILSTTSDNQLNSKDAQLKQPSKDIQTAL
                     QAIQAKYKNAKIKEISLANNKSNKLVYKVKLLDGNTPKIAQFDSDKASFIKANKLNTT
                     HKNHD"
BASE COUNT         4682 a         2322 c         2829 g         5339 t
ORIGIN      
        1 ttatgtattt ggagacgtta gccacccagt caaatatgac tgggaagatt tttagtatgt
       61 ctaaagaaga gttgtcatat ttacccgtta ttaaactttt taagaatcaa ggtatataca
      121 acggcttgat tggtctattc ctcctttatg ggttatatat ttcacagaat caagaaattg
      181 tagctatttt tttaatcaat gtgttgctag ttgctgttta tggtgctttg acagttgata
      241 aaaaaatctt attaaaacag ggtggtttac ctatattagc tcttttaaca ttcttatttt
      301 aatactactt agccgttcga tttagttgaa cggcttttag taatcatttt tttctcataa
      361 tacaggtagt ttaagtaatt tgtctttaaa aatagtataa tataactacg aattcaaaga
      421 gaggtgactt tggttatgac tgagaactgg ttacatacta aagatggttc agatatttat
      481 tatcgtgtcg ttggtcaagg tcaaccgatt gtttttttac atggcaatag cttaagtagt
      541 cgctattttg ataagcaaat agcatatttt tctaagtatt accaagttat tgttatggat
      601 agtagagggc atggcaaaag tcatgcaaag ctaaatacca ttagtttcag gcaaatagca
      661 gttgacttaa aggatatctt agttcattta gagattgata aagttatatt ggtaggccat
      721 agcgatggtg ctaatttagc tttagttttt caaacgatgt ttccaggtat ggttagaggg
      781 cttttgctta attcagggaa cctgactatt catggtcagc gatggtggga tattctttta
      841 gtaaggattg cctataaatt ccttcactat ttagggaaac tctttccgta tatgaggcaa
      901 aaagctcaag ttatttcgct tatgttagag gatttgaaga ttagtccagc tgatttacag
      961 catgtgtcaa ctcctgtaat ggttttggtt ggaaataagg acataattaa gttaaatcat
     1021 tctaagaaac ttgcttctta ttttccaagg ggggagtttt attctttagt tggctttggg
     1081 catcacatta ttaagcaaga ttcccatgtt tttaatatta ttgcaaaaaa gtttatcaac
     1141 gatacgttga aaggagaaat tgttgaaaaa gctaattgaa aaagtcaaat cactgacttc
     1201 tgtgattaaa attgtatttt ttatatctgt tttagtgctt attattgttg aaatgattca
     1261 tttgaaacga actatttctg ttgagcaact aaagagtgtt tttgggcaat tatctccaat
     1321 gaatcttttc ttaattatcc ttgtgggggt tatcgctgtc ttaccgacaa ctggatatga
     1381 ctttgtactg aatggacttt tacgtacaga taaaagcaaa aggtatattt tacagactag
     1441 ttggtgtatc aacactttta ataacttgtc aggattcggt ggcttaatcg atattgggtt
     1501 gcgcatggct ttttatggta aaaaaggtca agagaagagt gacctaagag aagtgactcg
     1561 ttttttaccc tatcttattt ctggtctgtc atttattagt gtgattgcct taatcatgag
     1621 ccatattttt catgccaaag ctagtgttga ttactattat ttggtattaa ttggtgctag
     1681 tatgtatttt cctgttattt attggatttc tggtcataaa ggaagccatt atttcggaga
     1741 tatgccatct agtactcgta taaaattagg tgttgtttct ttttttgaat ggggatgtgc
     1801 ggccgcagca tttataatta tcggttattt aatgggcatt catctaccag tttataaaat
     1861 tttaccacta ttttgtattg gttgtgccgt ggggattgta tcccttattc ccggtggatt
     1921 aggaagtttt gaactagttc tatttacagg gtttgctgcc gaggggctac ctaaagaaac
     1981 tgtggttgca tggttattac tttatcgttt agcctactat attattccat tctttgcagg
     2041 tatctatttc tttatccatt atttaggtag tcaaataaat caacgttatg aaaatgtccc
     2101 gaaagagtta gtatcaactg ttctacaaac catggtgagc catttgatgc gtattttagg
     2161 tgcattctta atattttcaa cagcattttt tgaaaatatt acttatatta tgtggttgca
     2221 gaagctaggc ttggacccat tacaagaaca aatgttatgg cagtttccag gtttattgct
     2281 gggggtttgt tttattctct tagctagaac tattgatcaa aaagtgaaaa atgcttttcc
     2341 aattgctatt atctggatta ttttgacatt gttttatctt aatttaggtc atattagttg
     2401 gcgactatct ttctggttta ttttactatt gttaggctta ttagtcatta agccaactct
     2461 ctataaaaaa caatttattt atagctggga agagcgtatt aaggatggag tcattatcgt
     2521 tagtttaatg ggagttctat tttatattgc aggactacta ttccctatca gggctcatat
     2581 tacaggtggt agtattgaac gcctgcatta tatcatagca tgggagccga tagcattggc
     2641 tacgttgatt cttactctcg tttatttatg tttggttaag attttacaag gaaaatcttg
     2701 tcagattggc gatgtgttca atgtggatcg ttataaaaaa ctacttcaag cttatggtgg
     2761 ttcttcggat agcggtttag cctttttaaa tgataaaagg ctctactggt accaaaaaaa
     2821 tggagaagat tgcgttgctt tccaatttgt aattgtcaat aataaatgtc ttattatggg
     2881 tgaaccagcc ggtgatgaca cttatattcg tgaagctatt gaatcgttta ttgatgatgc
     2941 tgataagcta gactatgacc ttgtttttta cagtattgga cagaagttga cactactttt
     3001 acatgagtat ggttttgact ttatgaaagt tggtgaggat gctttagtta atttagaaac
     3061 gtttactctt aaagggaata agtacaaacc tttcagaaat gccctaaata gagttgaaaa
     3121 ggatggtttc tatttcgaag ttgtacaatc gccacatagt caagagctac taaatagttt
     3181 ggaagagatt tctaatactt ggttagaagg acgtcctgaa aaaggtttct cattaggata
     3241 ttttaataaa gattatttcc aacaagcccc aatagctttg gtaaaaaatg ctgaacacga
     3301 agttgttgct tttgctaata ttatgccaaa ctatgaaaag agtattatct ctattgattt
     3361 aatgcgtcac gataaacaga aaattccgaa tggcgttatg gatttcctct ttttatcatt
     3421 attctcttat tatcaagaga agggatacca ctattttgat ttggggatgg cacctttatc
     3481 aggagttggt cgcgttgaaa caagttttgc taaagagaga atggcgtatc ttgtctatca
     3541 tttcggtagt catttctact catttaatgg tttacacaag tataagaaga agtttacacc
     3601 attgtggtcg gaacgttata tttcttgttc tcgttcgtcc tggttaattt gtgctatttg
     3661 tgccctatta atggaagata gtaaaattaa gattgttaaa taagctttat ttggcaatta
     3721 aaaagagcat gtcatgcgac atgctctttt taaatcattt aataccattg attgcttgaa
     3781 tctactttat aatatgatgt gcttttaaat attgtttagc tactgtagct gctgatttat
     3841 gctttacagc tacttggtag ttcatttctt gcatttcttt ttcagtgata tgaccagcaa
     3901 gtttattgag agcttttttt acttgagggt attttttaat cgtatcttgc cttagtagcg
     3961 gtgctccttg atatggcgga aatagatgct tatcgtcttt gagaatttga agttgatact
     4021 ggattaattc actatcggta gagtaagcgt caataatatt gacatcttta ctattgatag
     4081 cttggtatcg taaagcaggt tctaaggttg agatatcaag gtttaaacca taatgtttct
     4141 gtaaaccaat actcccatcc tctctatcct taaattctaa agtgaaacca gcttttagtt
     4201 tatctaattt ttttaggtca ctgatatttt tgagttgatt agcttcagcg taatcttttt
     4261 taacagctac agcgtaggtg ttttgataag ccataggtga caaaagtgac aacttatctt
     4321 gttttaaaat accgtttttg gctaagttgt aaacctgttt agggttatta cttactttag
     4381 gaggattttt aagtaaactt gatgtaatag tacctgtaaa ctctgggtaa agatcaatgt
     4441 caccagattt tagggcttga tataagaaag ttgtttttcc gaaattaggt ttaagcttaa
     4501 ctttgatatc tgtctgatcc tcaataagtt ctttgtacat attaattaaa atatctggtt
     4561 cagttcctaa cttccctgca acagttatcg ttgcagtatt tgttccaggc atccattttg
     4621 gagcatagga tagtcctaag cctgctagta aaagtatacc agaaacggct atggttcgta
     4681 atctagcctt ttctagtaat ccaattaaac cactaaatat aatagctaag acagcagaag
     4741 agattgcccc tatgagaatt agagaaggat tgttgcgatc aatccctaac aaaataaatg
     4801 atccaaggcc accagctccg ataagactag ctaatgtagc tgtaccaata atcataacag
     4861 aggcagttct aatgcctgag atgataacag gcatacttaa agctaactca aactttttaa
     4921 gcctttccca acgtgtcatt ccaaaagcag ttgctgcctc aatgagatta gcatcaattt
     4981 gcatcagtgc tgttacagta ttttgtaaaa ttggaaatag agcgtaaata attagtgcaa
     5041 cgacagctgg tacagttcca attcccataa aaggtataaa taaaccaagc aaggctaaag
     5101 aaggaattgt ttgaaatatc ccagttatct gaaggacaac atgactgatt tttttataat
     5161 gactaatgat gataccgagt ggaatagcaa ttaaggttgc taaaataagt gttagtagtg
     5221 atagctggag gtgttcaata agactttgag tccaatctcc aaaacgctct tgaaaggtag
     5281 tgattagtgt tgtcatactc agttctcctc ttcaaatagg gttctaacaa attggtcaga
     5341 gggattattt tgaattattt ctggtctatc caattgcact atcttacctt ccttaataac
     5401 acaaattcta tctgcaagac gcatagcttc tttcatgtca tgggtgacaa acactaatgt
     5461 aatacccaat tcagactgta attgtttggt gatatcttgc aattgacgtc ttgagatagg
     5521 gtctaaagca gaaaatggct catccataag tagtacctta ggtttagcaa cgattgctcg
     5581 taagatacct atgcgttgtt gttcaccacc agagagctca tgaggatagc gattaaagta
     5641 gtccttagca ggtaaaccca ccttatcaag aaggtcgcta gcggcttttt tttggtcacc
     5701 ttttgaccag cccttcattt cagggataag ttctatattt tctccaactg ttaaattagg
     5761 gaaaagtgca atttgttgca agacataacc agtttctaag cgtaattgtc gcaaatcaag
     5821 tgatgtaatg ttttccccat ctaaagtaat agcgccttgg cttggttcga tgagacgatt
     5881 aatcatcttg aggatggtag ttttaccact gccactggcc cccacaagga caaagaattc
     5941 tcctctttga atatggcaac taatattatc aatgatggta tgatcaccat atgatttact
     6001 aacattttca aatgaaatca tgtaatttac tttctagtgc gtaacgagtg taggtcgtat
     6061 gatattaggg ttatattagc ataaagtaga cattcaaaca actatctgac atttaaattg
     6121 tcagaaacga taaactagaa gcaaaaacaa acaccttgcg gtagacaagg tgtttgttgg
     6181 agatttatga aaagaaaagt tttaggatgg ttatagtata taaggccatg cttaaacaag
     6241 tcttaaaacg ttttactctg taaaataagt tcatcgaaaa aacgtttaag tgaaatttta
     6301 ccaccataca tgctttttcc ttgaataagt tgtgcctcat tatggatatt ctgaaatcca
     6361 aacaatgcat tagcatattg cagtgttaat tcattttcaa aatcatcaat gtacagatga
     6421 gccatattta tcatagcttt tttaacggca cgacgaatac gttgtaaaat gattttttgt
     6481 tggtgttcat cgatagaaag ttctttttga aaatcaaatt gggcaaaatt taattcttgt
     6541 tttaataaaa gttcacagat gttaagaata tctgtgtagg ctgtctctga agtaatacct
     6601 aaataactga gtattgaacg gattttagtg aggttactag tcgttatagg cttttgatat
     6661 gatggcgtat tttctaggag attttggatt gtatttaatt ttttttgcat ttcgatagta
     6721 tcagtgacac gtttcacgac gctttttact tcaataatat taatgggttt attaataaaa
     6781 aattcgattc ctgctttata tgcttcttgt cgtaaatcat tatctttgac ttgggaaatc
     6841 ataataaatt ttaaatcgga acgctgttta tagatttttt ggactaatgt tacaccgtct
     6901 aatattggca ttaataaatc aatcaatacg atatcaacat ctgcaatcag caattcgttg
     6961 tatgccttac tggagacatt attcactctg actactgtat tattgaaatc ttcttcaata
     7021 atatcttgta aaatcatagt gatagtgggg tcatcatcaa taatatagaa tttcacgtat
     7081 tatctcctaa cttaaatcga tttttacgga ataacagtgt aaatgttgtt ccatttggct
     7141 gattaggaca aacagttatc gttccttgat attgctcttc cattaaaata cgcacgtggg
     7201 atagcccgat tcctcgatag atatcaccat tagcatcaaa tttagtcgaa aatccaggtt
     7261 tgaaaatcat gtgtttcatt ttatcaggta ttcctggtcc gttatcacta atctcaattt
     7321 taataaaatc ttctaattct tctgtgtgaa cactgattgt accctttttt tgtttatcaa
     7381 tagcttctac tgcatttaaa acgatattgg aaataatggt tagtaaatag taatagtttg
     7441 atattatcaa attacattta ttattttcga gaataatgat atcttgatgc cgaaattgaa
     7501 taatactcct agtataagat aaaacaattt gaaagatatc tttcaatgcc atagttgatt
     7561 cattttttac actaaaatag gttcctaagc ctttaataat attttggtaa tctttcttaa
     7621 cttcgtgaac gtcccgcgaa atatctagag agaggtgttg gtattcttta ggtaggtgat
     7681 atttggataa ctctttatct aataaataag catttttcat gatgttttca atttcaatga
     7741 tatttttttg cataaaataa acctcgcttt tgacggctga agttgaccac ataaaataat
     7801 agtatcgacg ttcgtgatcg tctttttgta agagaagtga tagataacta taagtataag
     7861 ccacagcgca actaataaaa gctcgcaaaa gtgctatggc aaataaagta gcgaatatag
     7921 ttatagtata attgttaaac tttatgagaa aactaatttc tacaaggttg gcaaaataat
     7981 cacaaataat gatagaaaag aaaaaagtac ctttattacg gtaggaacga tgccaataaa
     8041 tagtataaaa ggtgatgcca taacaaatat aaaacgccat atcagttaaa acgaattcaa
     8101 ttatttgatg aatctcagcc tcgccagcga tacttaatat gattcctctg aagataggag
     8161 aagcaaaagt aattcctaga cagagaagaa ttggattaat gtcgtcatta aaatataaaa
     8221 agacaggcaa aatgaacaaa gataaggtca taataaaccc atcagcaagt attgagatat
     8281 ttatttgagc agctaaggcg ataactattg cagagataat aatacgctgc tttctttcta
     8341 gatgtttttc tgataatata tctagcatac aaatgtcctt aaagtttttt tgaagatttt
     8401 tgatgaattt tgattatttt tgaaagcgct atctacaata agagtataaa aaaaaaggag
     8461 gcaataagca atgaaaaatt tacgtaaccg tagttttctt actttattgg attttagtac
     8521 agcagaagtt gaatttcttt taaaactttc agaagattta aaacgtgcta aatacgcagg
     8581 aattgaacaa caaaaacttg ttggtaaaaa tatcgctttg atttttgaaa aagattctac
     8641 acggacacgt tgcgcttttg aagttgctgc tcacgatcaa ggagctcatg ttacttattt
     8701 aggtccaaca ggcagtcaga tgggtaaaaa agagacctca aaagacactg cgcgtgtgct
     8761 aggtggtatg tatgatggta ttgagtatcg tggattttct caagaaacag ttgagacttt
     8821 agccgaattt tcaggagttc ctgtgtggaa tggcttaacc gatgctgatc atcccacgca
     8881 agtactagcc gattttctga ctgctaagga atgcttacat aagccctata aagatattcg
     8941 ctttacgtat gtgggtgatg gacgtaacaa tgtcgctaat gccttgatga tcggtgcttc
     9001 tattgtaggc atgacttatc atttggtttg tcctaaagaa ttagagccag atcctgaact
     9061 actctctaaa tgtcaagaaa ttgcgaaaac tactggtgct tctattgaaa tcactgatga
     9121 tattgcagaa ggtgtcagag attcagacgt cctatataca gatgtttggg tttcaatggg
     9181 agaaccggat gaagtttgga aagaaagaat agctctttta gaaccctatc gtattaccca
     9241 agaaatgctt aatatgacag aaaatccaaa cgttattttc gagcattgct tgccctcatt
     9301 tcataatata gatactaaag ttggatacga catttatgaa aaatatggtt tgaaggaaat
     9361 ggaagtatca gatgaagttt ttgaaggccc tcactcagtt gttttccaag aagctgaaaa
     9421 tcgtatgcat actattaaag cagttatggt agcaacttta ggtgattagg aggaattttc
     9481 atggcaaaaa ttgttgttgc actaggagga aatgccttag gaaattctcc agaagaacaa
     9541 ttaagattag taaaacatac tgcaaaatca ttggtagcct tgataaaaaa gggacacgaa
     9601 attgttgtaa gtcatggtaa tggaccacaa gttggtgcca ttaatttagg aatgaatttt
     9661 gcggctgaga gtggtcaagg aactaatttt ccttttccag aatgtggagc aatgagccaa
     9721 ggatacatag gctatcatct ccaacagagc cttttaaatg aattacgtca agaaggaata
     9781 aataaggaag ttgctacaat tattacccaa attgaggtgg atgaatcaga tcaagcattt
     9841 agcgcaccta ccaagcctat aggtaccttt tatgataagg agacatcaga aaagattgct
     9901 atagagaaag ggtatacttt tgtagaagac gctggacgag ggtatcgtcg tgttgttgct
     9961 tcacctgaac ctaaaaaaat catcgaaatt aatagcatta aaacacttat tgaaaatgat
    10021 accctagtta tcgcaggtgg aggtggaggc atccctgtca ttaataaggg aggttatgaa
    10081 ggtattgcag ctgttattga taaagacaaa tctagtgcac tgttagcagg agaactagct
    10141 gcggatcaat tgattattct cactgcagtt gattatgttt atactcaatt tggtaaagaa
    10201 aatcaaaaag ctttaacaga ggttaatgag aatcaaatga ttgattacgt taaccaaggt
    10261 gagtttgcaa aaggaagcat gttacctaaa gtagtagcct gtatgagttt cttagatcac
    10321 aatccgaaag gaacagcctt aatcacctca ttaaatggtt tagaagatgc attagatggg
    10381 aaattaggaa cacgtataac taagtaatct cagataagtg caaagaaatg attatttaac
    10441 cttcatcttt gcactttttc tgatgaagtc aatctatttt tattatagag gaggcgtgtc
    10501 atgccagaaa aaatgacaaa cagtgaaaac aaaaagagta aaggttttcg aatgccaggt
    10561 gcctttacaa tattattcat tttaacaatc ttttcggttt tggctacatg gtggatacct
    10621 gctgggtcat attcaaaatt acaatttgat acagcatcat ccaaactagt agtaacagat
    10681 ccaaatggta agacagttca tgttccagca acgcaaacac agttggataa gatgaatgtc
    10741 aaaattaaaa ttaaagaatt tacttctggt gctattagta agccagtttc agttccaaat
    10801 acttacaaac gtttgaagca aaatccggca ggcattggct ccgtgacgac tagtatggta
    10861 aatggaacca ttgaagctgt tgatattatg gtatttatca tggtacttgg aggcatgata
    10921 ggagtagtta gaaagagtgg cgcttttgag tctggtcttc tagctttaac aaagaaaacc
    10981 aaaggtagag agtttttatt gatattttta gtttccttat tgatggtttt aggcggtact
    11041 ctatgtggta ttgaagaaga agcagttgct ttttatccta ttttagttcc aatcttttta
    11101 gccatgggtt atgattctat catttgtgta ggtgctattt tcttagcaag ttctgttggg
    11161 acatcatttt caacaattaa tccgttttct tctgttattg cttcaaatgc agctggtatt
    11221 agtttcacag aaggcttatc atggcgtaca gtagggtgta ctgcaggtgc tatttttgtt
    11281 gtggtgtatc tacattggta ttctaaaaaa ataaaagcga atccagagtt ttcatattcc
    11341 tatgaagata gagtagaatt taatgctaaa tggggcatga caactaacca taccccctca
    11401 ctctttactg tcagacaaaa aattatttta agtttatttg ttatttcatt tcctttgatg
    11461 gtttggggag taatgtcaca gggatggtgg tttccaacca tggcttcttc ctttttagct
    11521 attacaatta ttattatgtt tttaacagca actggggcaa atggtattgg agaaagggat
    11581 gttgttgatg agtttgttaa tggtgcatca agccttgttg gggtttcatt aatcattggt
    11641 ttagcgcgtg gtatcaatat tattttgaac caaggttata tatcagatac gatgctttac
    11701 acagcttcaa aattagcttc acatgttagt ggccctgtct ttatcattgt tatgatgttt
    11761 atctactttg tgttaggatt tgttgttcca tcctcgtcgg gtttagcagt actttcaatg
    11821 ccaattctag cacctcttgc agatactgtt gggattcctc gttcagtagt agtaatggct
    11881 tatcaatttg ggcagtatgc aatgttgttt ttagcaccta ctggactagt tatggcaacc
    11941 ttgcagatgt tagatatgaa gtactcccat tggctaaaat ttgtgtggcc ggttgtttta
    12001 ttcctcctaa tatttggtgg cggtttgtta gttctacaag ttttaatact ttaaagattt
    12061 cagctattag tctcaaaaat atctgaagag atataataaa ttgagtttaa gttaaataaa
    12121 aaaacgatag gtgtaatcta tcgttttttt atttcctttc tctagtcttt cttcaatttt
    12181 ggaaaggtga gcgtaaactg actgccatca tcaggtttag aatcaaccca tattttagca
    12241 cggtgtattg tagcgatttg ttttactaaa gatagcccta aaccaattcc agactgacta
    12301 tccttggttc gagcagagtc cacttgatag aaacggttcc aaattttact ttggtcatca
    12361 actgagatac ctagaccatt atctttaata gagaagacaa tttgattatc ggactgtctt
    12421 agactaatag aaatatgatt gtttgtaaat ttaatagcat ttgaaaatag attatcaaaa
    12481 agccgtttga gtagtgtttg ttgacctata atccaaaggt catcttcgat ttgagagtca
    12541 aggtgtatag gggtattatc taataatcta cttgaatcgg atactagttg cttcatcata
    12601 attgagaaat taagaggttc taactgaatt gataatggat tttctgcctt agtcaattct
    12661 agaagttgct caactaattt tttcattgat agtgactgac ggtgtataac ctcaaaggat
    12721 tcttttgctt caattaaatt ttctgcgtag ttttttccgt attcactttc agaaagtata
    12781 actgttacag gagtccgtag ttcatgagaa acatcattgt taaattgttt ttcccgaaca
    12841 aatgatgatt caatagaagc caacatagta ttgataacag ctgctagctc agttaattca
    12901 ttttcgttat ttttagtgat aattcgctta gtatagtcct cattttgggt aataaattga
    12961 gcggtttcag taatttcttg aatcggtcta aaagcgcgct ttaaaattaa atagccacca
    13021 acagttatta taatcaatat aagaggaaga acgataccca aactaaataa caattcagtt
    13081 aattgcttat ccagtctgtc aacagtacgg atagcacgta accactgatt tttcccagaa
    13141 attggaatat caacatactg aaatgtacga ttctccatgg aaaatagaga taatttcttt
    13201 ttatgtttat gttgatggtc taaatccttt ttaaatcctt ttggaaaacc agaatagata
    13261 acttttccat tttggtcata gactgaataa aatattccat cttcaaaagc ctcataccga
    13321 tgacctttag ctaacgcttg actcatttgc acagctttag cttctaaatt tttttcagat
    13381 ttgttttcag caatgctttt tgaaataaaa aatgaaccaa ttaatacacc gataacaagg
    13441 ataaaaataa aagagctgta ccagagggta atttgagtga tgatgctaga ctttttaggg
    13501 taatttattt tactttggaa taacatatcc taaacctcgt ttagttttta ttagtgatcc
    13561 gtctcggttg ttatctagtt ttcgacgaag gtttttgata aggacatcaa taatattgga
    13621 ctctccatca taatcataat cccaaacgtg ttcacgaatt tgatgacgag agaggacttg
    13681 gtctctgtga cgtgccaaat actcaaggac ttcgtattcc ttagctgtta aatcaatcaa
    13741 attgttgttt ctccaaactt gctttgtaga taaattgatt gttatatctt gaatttgaat
    13801 atcatcagaa gatacttgtc tatttgtacg tcgtaacata gctcgaattc gggccatgag
    13861 ctctccaaat tcaaatggtt ttacaaggta atcatccgca ccaaaatcta gacctgcgat
    13921 acgatcttca acggcatctc gagcagtcaa cataagaata gatacttgac ttcctttgtt
    13981 gcgaagcagt tttaaaaagg tgaagccgtc catttttggc atcattacat ctaagataat
    14041 cacatcatat tctgccactg aaacaaattg taaggcttct tcaccatttg aagcactgtc
    14101 aacactgtag tgttgctttt ttaaaagttt tacaatacta cggttcaagt caaactcatc
    14161 ttcaacaaca aggattttca ttttttggcc tccgtatggc ttttttccat tatatcacaa
    14221 aagggaatgg agtactgagg tcctattccc tttaatacac tagtattagt cgtgattttt
    14281 atgagttgta ttaagcttat ttgcctttat aaatgatgct ttatcactat caaattgggc
    14341 aatcttagga gtatttccat caagtagttt tactttataa actagcttat ttgatttgtt
    14401 attcgcaaga gaaatttctt ttatttttgc atttttatat tttgcttgaa tagcttgtaa
    14461 tgcagtctga atatctttac ttggttgttt taattgggca tcttttgaat tgagttggtt
    14521 atctgatgtt gttgaaagga tcttcccaga attagcatca attttatatt ttttagctat
    14581 tttattatca ataactttga tttcataaaa aggtgtatca cctttggtat ttaattcaat
    14641 ttgtgaaata aagcctgagc gagcttcttt tttggcagtt tctaaggcag tcttataatc
    14701 gatttttgtc gagatattct ttaatgtttt tagtgagtgt ttttctctat gatcgtgatg
    14761 tttatcgtgt ccttcatgct tatgttcaaa agacatgtgt tctgagtgat ggttatgcgc
    14821 taatgcagct ccgcttactc ctatagctcc cccaatacca agtagcgtaa tggaagctaa
    14881 ccctacgtat aatttattgc tcatgaattt ttttaagttg tttttatagt ttgtcatact
    14941 gacttccttc tttctttttt caaaccttgg attaggtttg ttacttctga gtatatagtc
    15001 gtaaaatgaa tctaaaatga atttttaaaa ttaggaacaa tattttcagt tataaaaaag
    15061 tgagttttaa aagaatgact aactacctga aggtaagagg taaatttata cttttttgtc
    15121 agattttatt tttaaaaata aaaagcccag aaatattgat ttcataggct tt
//
LOCUS       DATRNK010000049        14907 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.049, whole genome shotgun sequence.
ACCESSION   DATRNK010000049 DATRNK010000000
VERSION     DATRNK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14907
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.049"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            192..1388
                     /gene="tuf"
                     /locus_tag="VBC52_001497"
     CDS             192..1388
                     /gene="tuf"
                     /locus_tag="VBC52_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEO6883077.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPTSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDEKYEDIIMELMSTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEVEIVGIKEDIQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVLAKPGSINPHTKFKGEVYILSKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIEVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
     gene            1569..2327
                     /locus_tag="VBC52_001498"
     CDS             1569..2327
                     /locus_tag="VBC52_001498"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEO6883078.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFIEAVASKLPSSELVEAGIAAP
                     ALTLSTVLEAAKGSELKIAAQNSYFENSGAFTGENSPKVLAEMGTDYVVIGHSERRDY
                     FHETDQDINKKAKAIFANGLTPIICCGESLETYEAGKAVEFVGAQVSAALAGLSEEQV
                     SSLVIAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQAVADKVRVQYGGSVKPE
                     NVAEYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            2504..3196
                     /locus_tag="VBC52_001499"
     CDS             2504..3196
                     /locus_tag="VBC52_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEO6883079.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIQ
                     AAGIEFDLAFTSVLKRAIKTTNLALEAADQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPDMAKDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKQLSDDEIMDVEIPNFPPLVFEFDE
                     KLNLVSEYYLGK"
     gene            3325..5370
                     /locus_tag="VBC52_001500"
     CDS             3325..5370
                     /locus_tag="VBC52_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEO6883080.1"
                     /translation="MLNRKKRYRLTVKKQNASIPRRLNLLFFIIVLLFTVLILRLEQM
                     QIGQQSFYMKKLTALTSYTVKESKARGQIFDAKGVVLVENDERPTVAFSRGNNISSQS
                     IKELANKLSHYITLTEVASSDRAKRDYYLADKANYKKVVESLPDSKRYDKFGNHLAES
                     TVYANAVAAVPVSAINYSEDELKVVALFNQMNATPTFGSVKLSTGELSDDQIKKLDAD
                     KKELLGISVTSNWHRRKKGTSLSDILGTISTEKAGLPREEVKKYLKKGYSLNDRVGTS
                     YLEKQYEDDLQGIRQIRKVVVNKKGKVVSDNITQEGKSGRNLKLTIDLNYQNKVESIL
                     KQYYGSELSSGRASFSEGMYAVAIEPSTGKVLAMAGLKNDHGNLVDDSLGTIAKNFTP
                     GSVVKGATLSSGWENKVLRGNEVLYDQEIANIRSWFTRGLTPISAAQALEYSSNTYMV
                     QVALRLMGQDYNTGDALTDRGYQEAMAKLRKTYGEYGLGVSTGLDLPESEGYVPGKYS
                     LGTTLMESFGQYDAYTPMQLGQYISTIANNGNRLAPHVVSDIYEGNDSNKFAQLVRSI
                     TPKTLNKIAISDQELAIIQEGFYNVVNSGSGYATGTSMRGNVTTISGKTGTAETFAKN
                     VNGQTVSTYNLNAIAYDTNRKIAVAVMYPHVTTDTTKSHQLVARDMIDQYISQFTGQ"
     gene            5385..5981
                     /gene="recR"
                     /locus_tag="VBC52_001501"
     CDS             5385..5981
                     /gene="recR"
                     /locus_tag="VBC52_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEO6883081.1"
                     /translation="MLYPTPIAKLIDSFSKLPGIGTKTATRLAFYTIGMSDEDVNEFA
                     KNLLAAKRELTYCSVCGNLTDDDPCLICTDKTRDQSVILVVEDSKDVSAMEKIQEYNG
                     LYHVLHGLISPMNGISPDDINLKSLITRLMDGQVTEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            6122..7168
                     /locus_tag="VBC52_001502"
     CDS             6122..7168
                     /locus_tag="VBC52_001502"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEO6883082.1"
                     /translation="MSKETLILLYGGRSAEREVSVLSAESVMRAINYDKFFVKTYFIT
                     QVGQFIKTQEFDEMPSSDEKLMTNQTVDLDKMVRPSDIYDDNAIVFPVLHGPMGEDGS
                     IQGFLEVLRMPYVGTNILSSSVAMDKITTKQVLATVGVPQVAYQTYFEGDDLEHAIKL
                     SLETLSFPIFVKPANMGSSVGISKATDESSLRSAIDLALKYDSRILIEQGVTAREIEV
                     GILGNNDVKTTFPGEVVKDVDFYDYDAKYIDNKIIMDIPAKVDEATMEAMRQYASKAF
                     KAIGACGLSRCDFFLTKDGQIFLNELNTMPGFTQWSMYPLLWENMGLTYSDLIEKLVM
                     LAKEMFEKRESHLI"
     gene            7315..8682
                     /locus_tag="VBC52_001503"
     CDS             7315..8682
                     /locus_tag="VBC52_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEO6883083.1"
                     /translation="MKLSLHEVAKVVGAKNQVSEFEDVPLGNIEFDSRNISEGDLFLP
                     LKGARDGHEFIEMAFDNGAIATISEKEIEGHPYLLVSDALKAFQVLAQYYIEKMNVDV
                     IAVTGSNGKTTTKDMIAAILSTTYKTYKTQGNYNNEIGLPYTVLHMPEDTEKIILEMG
                     QDHLGDIHVLSEIAKPRIAVVTLIGEAHLEFFGSREKIAEGKMQITDGMSSDGILIAP
                     GDPIIDPYLPANQMTIRFGHDQELQVTELKEEKHSLTFKTNALEHQLRIPVPGKYNAT
                     NAMVAAYVGKLLAVAEEDIVDALENLQLTRNRTEWKKSANGADILSDVYNANPTAMRL
                     ILETFSAIPNNDGGKKIALLADMKELGEQSVDLHNQMIMSIRPDSIDTLICYGQDIEG
                     LAQLASQMFPIGKVYFFKKNQEVDQFDQLLAKVKDTLKEKDQILLKGSNSMNLSKIVD
                     ILEVG"
     gene            8869..10089
                     /locus_tag="VBC52_001504"
     CDS             8869..10089
                     /locus_tag="VBC52_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEO6883084.1"
                     /translation="MKNLNRYVVAVSGVVLHLMLGSTYAWSVFRNPIISETGWDISSV
                     SFAFSLAIFCLGMSAAFMGHLVERFGPRIMGMISAILYGAGNVLTGLAIETQQLWLLY
                     VAYGILGGIGLGSGYITPVSTIIKWFPDRRGLATGFAIMGFGFASLVTSPLAQSLMIR
                     IGVGKTFYILGLVYFFVMMIASQFIKQPPQEKITILTHDGKKNAMNSQIITGLKANAA
                     IKSKTFYIIWLTLFINISCGLGLISAASPMAQDLAGYSAESAALLVGVLGIFNGFGRL
                     LWASLSDYIGRPLTFIILFIVNFIMTSSLFLSFNAIVFAIAMSILMTCYGAGFSLLPA
                     YLSDIFGTKELATLHGYSLTAWAIAGLFGPLLLSKTYSWGNSYQLTLMVFGFLFLFGL
                     LLSLYLRKLTTKVV"
     gene            10218..10904
                     /locus_tag="VBC52_001505"
     CDS             10218..10904
                     /locus_tag="VBC52_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HEO6883085.1"
                     /translation="MIEFFSNIRTEIPQMPLLIHSLILSVLPFLMWLTLVNRDKPLYK
                     TIWSILLGLQLITIYTWFFWAKLPLSESLPLYHCRIGMFVVLLARPGILKDYFALLGV
                     VGGVLAMIHPDFYPYQFLHVTNIFFFIGHFALFVLSLLHLMTQSNLDKLNPKLIIQLT
                     LLINMSLIFINLLTGGNYGFMMKTPILGITNPFLNLFIVTTLLSFLVLFVKQIFQKVR
                     MRASLKTEES"
     gene            11083..12621
                     /locus_tag="VBC52_001506"
     CDS             11083..12621
                     /locus_tag="VBC52_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6883086.1"
                     /translation="MNKSFNTKLGLVTVAVLSGIVLTSQLPVNAKAETPVMAASAQQG
                     FRFVATVVDSQTHVLPGKLVTLSEVTSGQPKIIASVKTNDAGQAIFNNLPIKRNLSVS
                     VDGQVKGYTIRTDVAGSSKAASFTATGVGTNEPTYSKKTIDITVRDQNAEPVSGRTVT
                     LKTQAGREIASLVSGDNGLTRFTDRLLDGTFYQYFVDGKKIGDIVPGESRSAYVNVAP
                     KKDYFTFTVTALDKDGLVVKGKEVTLTDITDGKAVALASLKTNDNGQAIFTNLPLSRN
                     ISVSIDGKSKGYTLRTDVAGSHKAAAFYVDGKGTKAPQFSAEAAVVTVYDANGNTIAN
                     QEVTLTNSNGTIVAKGLTEKDGKARFANKLMAGTLYNIFVNGIEMPKTALVGSDVSVF
                     LTDKQIKKEKPVTPDKLDKEMNQEKEKELLDTPKPVPPTSKPTAPIKNKKDDSPKKFT
                     KSQSSSLKSSVKAIGAKPKAMTALAKKLPKTGDQAISILTLVGFIVTGLAIMLGFLKK
                     SRSK"
     gene            12798..14342
                     /locus_tag="VBC52_001507"
     CDS             12798..14342
                     /locus_tag="VBC52_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEO6883087.1"
                     /translation="MTLQDEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLDLLEELEEVLG
                     IASYPMNWPIGMGKSFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VIDPLAKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPELFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWINSDDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     KLEEKM"
     gene            14428..14808
                     /locus_tag="VBC52_001508"
     CDS             14428..14808
                     /locus_tag="VBC52_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEO6883088.1"
                     /translation="MGIFSGLMGNASQMDTDKVENQLSDILISDEQVDLAYTLIRDLI
                     VFTNYRLILVDKQGVTGKKVSYNSIPYASISRFTVETSGHFDLDAELKIWISSAIEPA
                     EVLQFKNDRNIVSIQKALATAVLK"
BASE COUNT         4876 a         2350 c         2971 g         4710 t
ORIGIN      
        1 taaaaaatac atataagaga taacaaatgc ctcaatcaat cttacccgaa ttttaaggaa
       61 ataaagaaat aagcaaaaaa gcataaaata cttgcttttt tagaggattt tgttaaaata
      121 gataagtaaa gttagactgt attgcctacc gtctatctat aaaatatatt ttattggagg
      181 cttttcctaa aatggcaaaa gaaaaatacg atcgtagtaa accacacgtt aacattggta
      241 caatcggaca cgttgaccac ggtaaaacta ctttaacagc agctatcaca actgtattgg
      301 cacgtcgctt gcctacttca gttaaccaac caaaagatta cgcttctatc gatgctgctc
      361 cagaagagcg cgaacgcgga atcacaatca acactgcaca cgttgagtac gaaactgaaa
      421 aacgtcacta tgcccacatc gacgctccag gacacgcgga ctacgttaaa aacatgatca
      481 ctggtgctgc tcaaatggac ggagctatcc ttgtagttgc ttcaactgat ggaccaatgc
      541 cacaaactcg tgagcacatc cttctttcac gtcaagttgg tgttaaacac cttatcgtat
      601 tcatgaacaa agttgacctt gttgatgatg aagaattgct tgaattggtt gaaatggaaa
      661 ttcgtgacct tctttcagaa tacgacttcc caggtgatga ccttccagtt atccaaggtt
      721 cagctcttaa agcacttgaa ggcgacgaaa aatacgaaga catcatcatg gaattgatga
      781 gcactgttga tgagtacatt ccagaaccag aacgtgatac tgacaaacct ttacttcttc
      841 cagttgaaga tgtattctca atcactggac gtggtacagt tgcttcagga cgtatcgacc
      901 gtggtactgt tcgtgtcaac gacgaagttg aaatcgttgg tattaaagaa gatatccaaa
      961 aagcagttgt tactggtgtt gaaatgttcc gtaaacaact tgacgaaggt cttgcagggg
     1021 acaacgttgg tgttcttctt cgtggtgttc aacgtgatga aatcgaacgt ggtcaagttc
     1081 ttgctaaacc aggttcaatc aacccacaca ctaaatttaa aggtgaagtt tacatccttt
     1141 ctaaagaaga aggtggacgt catactccat tcttcaacaa ctaccgtcca caattctact
     1201 tccgtacaac tgacgtaaca ggttcaatcg aacttccagc aggaacagaa atggttatgc
     1261 ctggtgataa cgttactatc gaagttgaat tgattcaccc aatcgccgta gaacaaggta
     1321 ctactttctc aatccgtgaa ggtggacgta ctgttggttc aggtatcgtt tcagaaatcg
     1381 aagcttaatt taacttaatt aagttcccaa acggcagtta gagtcagaca gctcaaattg
     1441 tgaagagtcc tcagggactc ttttttagta tctataatta aagcgtttta ctagactcgt
     1501 gaaactttta attaactata atttatggta tgatagatta gtaaataaaa aaagaagagg
     1561 tatgtgtaat gtcacgtaaa ccatttatcg ctggtaactg gaaaatgaat aaaaatccag
     1621 aagaagcaaa agcattcatc gaagcagtag cttctaaatt accatcatca gaacttgttg
     1681 aagcagggat tgctgctcca gcgcttactt tatcaacagt tcttgaagca gctaaaggtt
     1741 cagaattgaa aatcgctgct caaaatagct attttgagaa ttcaggtgca ttcactggtg
     1801 aaaacagccc taaagtattg gctgaaatgg gtacagacta cgttgttatc ggtcactcag
     1861 aacgtcgtga ttatttccat gaaactgacc aagatatcaa caaaaaagca aaagctatct
     1921 ttgctaatgg tttgactcca atcatttgtt gcggtgagtc tcttgaaact tacgaagctg
     1981 gtaaagcggt tgaattcgta ggtgctcaag tttcagcagc tcttgcaggt ttgtcagaag
     2041 agcaagtatc atcacttgtt atcgcttacg aaccaatctg ggctattggt actggtaaat
     2101 cagcaactca agacgacgca caaaacatgt gtaaagcagt tcgtgacgtt gttgcagcag
     2161 acttcggtca agcagttgct gacaaagttc gcgttcaata cggtggttca gttaaacctg
     2221 aaaacgttgc agaatatatg gcttgcccag acgttgacgg tgcccttgta ggtggagctt
     2281 cacttgaagc agaaagcttc cttgccttat tagattttgt taaataaatc acatgatatc
     2341 taaaggcata gcactctagg tgatatgcct tttcttatag atagttaata ataagctatt
     2401 tttgattatt gaactagtaa tcagactaga tttttatggc tattttttgt agaatagtta
     2461 aagtaaatat tggtatagcc aaaattaaag gagaaatttt ctaatggtaa aattagtatt
     2521 cgcacgccac ggtgaatctg agtggaataa agctaacctt ttcactggat gggctgacgt
     2581 agatctttca gaaaaaggta cacaacaagc tattgatgct gggaaattaa ttcaagcagc
     2641 aggtattgag ttcgaccttg cttttacatc agttcttaaa cgtgccatca aaacaactaa
     2701 ccttgccctt gaagcagctg atcaactttg ggtaccagtt gaaaaatcat ggcgcttgaa
     2761 cgaacgtcat tacggtggat tgacaggaaa aaataaagca gaagcagctg aacaatttgg
     2821 tgatgagcaa gttcatattt ggcgtcgttc atatgatgta ttgcctccag atatggctaa
     2881 agatgatgaa cattcagcac atactgatcg tcgctatgct tcactagatg attctgttat
     2941 tccagatgca gaaaacctaa aagttacttt agagcgtgct cttcctttct gggaagataa
     3001 aattgctcct gctcttaaag atggtaaaaa tgtgtttgtt ggtgcacacg gtaactcaat
     3061 ccgtgctctt gtaaaacata tcaaacaatt gtcagatgat gaaatcatgg acgttgaaat
     3121 tcctaacttc ccaccacttg ttttcgaatt tgatgaaaaa ttaaaccttg tttcagaata
     3181 ttacttaggt aaataatcta atctttatat aaaaaattca ggccaagttg gcttgaattt
     3241 tttattattt ataacatttt gagtgtctgt aaaaaaccat ttttatgata caatagtagt
     3301 cagagttttt atagcaagga gttaatgttg aatcgtaaaa aaaggtatcg tctcactgta
     3361 aaaaagcaaa atgctagcat acctagacga ttaaatcttc tattttttat tattgtttta
     3421 ttatttactg ttctaattct tcgtttagag caaatgcaga ttgggcaaca atcattttac
     3481 atgaaaaaac tgacagctct tacgagttat acagtgaaag aatcaaaagc gagaggtcag
     3541 atttttgatg ctaagggagt tgtcttagtt gaaaatgatg aacgtcctac tgtggcgttt
     3601 tctagaggaa acaatatttc atctcagtct atcaaagagt tagcgaataa attatcacat
     3661 tacattacct taaccgaagt tgcttcaagc gatagagcaa agagagatta ttatttagca
     3721 gataaagcca actacaaaaa agttgttgaa agtttaccag attctaaacg ttacgataaa
     3781 tttggtaacc atctagccga atcaaccgta tatgcaaatg cagttgctgc agttccagtg
     3841 agcgctatta actattctga agatgagtta aaagtggttg ccttatttaa ccaaatgaat
     3901 gcgacaccaa cgtttggttc agtgaaatta agtacaggcg aattaagtga tgatcaaatc
     3961 aagaaactgg atgctgataa gaaggaactg ttaggaattt ctgtaacaag taattggcat
     4021 cgtcgtaaaa aaggaacttc tttatcggat atattaggta ctatttctac agaaaaggca
     4081 ggtttaccaa gagaagaagt taaaaaatat ttgaaaaaag gctattcact gaatgaccga
     4141 gtgggaactt cttaccttga aaaacaatat gaagatgact tacaaggaat cagacaaatt
     4201 cgcaaagtag ttgttaataa aaaaggtaaa gttgtttcag ataatattac gcaagaaggt
     4261 aagtcaggaa gaaatcttaa attaacgatt gatcttaatt accaaaacaa agtagaaagt
     4321 atcttgaaac aatactatgg cagtgaatta tctagtggac gcgctagttt ttccgaaggg
     4381 atgtatgcag ttgctataga accttcaact ggtaaagtgt tagcaatggc tggtctgaaa
     4441 aatgatcatg ggaatttagt tgatgacagc ctaggcacta ttgctaagaa tttcacgcca
     4501 ggatctgttg tgaaaggtgc gacgctatca tcaggatggg aaaataaagt tcttagagga
     4561 aatgaagtgc tttatgacca agaaattgct aatatacggt catggtttac acgaggtttg
     4621 actccaatat ctgcggcaca agcgctagag tattcatcta atacttatat ggttcaagta
     4681 gcacttcgtt taatgggaca agactataat acaggagatg ctttaactga tcgaggctat
     4741 caagaagcaa tggctaaact aaggaaaact tacggcgaat atggtttagg ggtttctaca
     4801 ggattagatt tacctgaatc agaaggttat gtacctggaa aatacagctt aggaacaact
     4861 ctaatggaat cgttcggtca gtatgatgcc tatacaccaa tgcaacttgg tcagtatatc
     4921 tcaactattg cgaataatgg gaatcgttta gcacctcacg tggtttcaga tatctatgaa
     4981 gggaatgatt ctaataagtt cgctcaattg gttcgttcaa tcactcctaa aacactaaat
     5041 aagatagcta tctcagatca agagttagcc attattcaag aaggttttta taacgttgtc
     5101 aatagtggaa gtggctatgc aactggtacg tcaatgaggg ggaatgtgac aaccattagt
     5161 ggtaaaactg gtaccgctga aacatttgct aaaaatgtaa atggacaaac agtttctacc
     5221 tacaacttaa acgctattgc ctacgatact aatcgtaaaa tagcagtagc ggtaatgtat
     5281 ccgcatgtta caactgatac aacaaaatcc catcaattag ttgcacgaga tatgattgat
     5341 caatatattt cacagttcac aggacaataa gagaggacat ttgaatgctt tacccaacac
     5401 caattgctaa attgattgac agcttttcaa aattaccggg tataggtact aaaacagcaa
     5461 cacgtttagc tttttacacc attggtatga gtgatgaaga tgttaatgaa tttgctaaga
     5521 atttattagc tgctaagcgg gaattaacct attgttctgt ttgtggtaat ttgactgatg
     5581 atgatccttg cttaatttgt acagataaga cgcgtgacca gtctgttatc ttggttgttg
     5641 aagatagtaa agatgtgtca gctatggaaa aaattcaaga gtataatggt ttgtatcatg
     5701 tcttacatgg cttaatttca cctatgaatg gtattagccc tgatgacatt aatttgaaaa
     5761 gtttaattac tcgattgatg gatggtcagg tgacagaagt tattgttgct accaatgcga
     5821 ctgctgatgg tgaagctaca tcaatgtata tttcacgagt cttaaaacca gctggtatca
     5881 aagtgacacg attagcgaga ggtttagctg ttggttcaga tatcgaatat gcagatgaag
     5941 taaccttgct tcgagcaatc gaaaatcgta cagagttata attgttataa agaaaaggtt
     6001 gggaatctcc taactttttc ttttacaata ctaactttaa gtggattgat ttttgaaagt
     6061 taggttaaaa tatggtatta ttaaaaggtc aatcttagat tagatagaaa agagaagaaa
     6121 tatgtcaaaa gaaacattga tcttgcttta cggtggtcgc tctgcggaac gtgaagtatc
     6181 tgtactgtct gcagaaagcg tcatgcgtgc tattaattat gataaatttt ttgttaaaac
     6241 ttattttatc acgcaagtag gtcaatttat taaaacacaa gaatttgatg aaatgccatc
     6301 ttcagatgaa aagttaatga caaaccaaac tgttgattta gacaaaatgg ttcgtccaag
     6361 tgatatctat gatgataatg caattgtttt ccccgtttta catggaccaa tgggggaaga
     6421 tggttctatc caaggatttt tagaagtttt aaggatgcct tatgttggga ctaatattct
     6481 atcttcaagc gtggctatgg ataaaattac aacaaaacaa gtccttgcaa cagtaggtgt
     6541 acctcaggtt gcatatcaaa cttattttga gggtgatgat ttggaacatg cgattaaact
     6601 ctctttagaa actttaagtt tcccaatttt tgtaaaaccg gctaatatgg ggtcatcagt
     6661 aggtatttca aaagcgacag atgaatcctc acttcgctct gcaattgact tagctctcaa
     6721 gtatgatagc cgtattttga ttgaacaagg cgtgacagct cgtgaaattg aagtaggtat
     6781 tttaggcaat aatgatgtta agacaacttt tcctggcgaa gtcgttaaag acgtcgattt
     6841 ctatgactat gacgccaaat atattgataa taaaattatt atggatattc cagctaaagt
     6901 tgatgaagca actatggaag caatgcgtca atatgcaagt aaagctttta aagcaatcgg
     6961 ggcttgtggt ttatcacgct gtgatttctt tttgacgaaa gatggacaaa tcttcttaaa
     7021 cgaactgaat acaatgcccg gttttactca gtggtcaatg tatcccctgc tttgggaaaa
     7081 tatggggcta acttatagtg atttgattga aaagttagtt atgttagcta aagaaatgtt
     7141 tgaaaaacgt gagagtcatt taatttaaaa aagttcaact aagtctttct tagttgagct
     7201 ttttcaaatc tatgatatgt tatgaaagtg tgttataatt taggagtata ttcgtttata
     7261 agggatagca tactatctac aacattgttg ctaaataata gaaaaggaaa tgatatgaag
     7321 cttagtttgc atgaagttgc taaagtagtt ggcgctaaaa atcaagtatc agagtttgaa
     7381 gacgttcccc tgggaaatat tgaatttgat agtcgtaata tcagtgaagg tgacttattt
     7441 cttcccttaa aaggagcaag agatggacat gagtttatag agatggcttt tgataatgga
     7501 gcaatagcaa ccatttcaga gaaggaaata gagggtcatc cttatctttt agtgtctgat
     7561 gcacttaaag catttcaagt cttggcacag tattatattg aaaagatgaa cgttgatgtt
     7621 attgcagtaa ctggctcaaa tggtaagaca acaactaaag atatgatagc tgcaatctta
     7681 tcaaccacat ataagactta taaaacacaa ggaaactaca ataacgagat tggcttaccc
     7741 tatactgtgc tacacatgcc agaagatact gaaaaaatta ttttggagat gggacaggat
     7801 catcttggag atatccatgt gttatctgaa attgcaaaac cacgtattgc tgttgttact
     7861 ttgattggtg aagcacactt agaatttttt ggtagtcgag aaaagattgc agaagggaaa
     7921 atgcaaatca cagatggtat gagttcagat ggcatattaa ttgcaccggg agatccaatt
     7981 attgatccat atcttccagc aaaccagatg actattcgtt ttggtcatga ccaagaattg
     8041 caagtgacag aactcaaaga agaaaaacat tccttaactt ttaaaactaa tgctttggag
     8101 caccaattac gtataccagt acctggtaag tacaatgcaa ctaatgcgat ggtcgctgcc
     8161 tatgttggaa aattattagc agtagcagaa gaagatatag tggatgctct agaaaatctt
     8221 caactaactc gtaatcgcac cgaatggaag aaatcagcca atggagcaga tattttatca
     8281 gatgtttaca atgctaaccc aacggctatg cgattgatac tagaaacctt ttcagctatt
     8341 cccaataatg atggtggaaa gaagattgct cttttagctg atatgaaaga actgggagaa
     8401 caatctgtgg atttacataa tcaaatgatt atgagtatca gacctgactc tattgatact
     8461 ttgatttgtt atggccagga tattgagggg ttggctcaat tagcaagcca gatgttccca
     8521 attggtaaag tgtatttctt taaaaagaat caagaagtag atcagtttga tcaattgctt
     8581 gcaaaagtaa aagatactct taaggagaaa gatcaaatat tattaaaagg ttctaactca
     8641 atgaatcttt ctaaaatagt tgatattctt gaagtaggat aatagaggtg ttagggtaac
     8701 aagttaccct aacttttttg ttttttaatc acgaataata ttcagtttta ttgaggaaaa
     8761 tagatatgct aagcagaaaa ctggtgatat attcagtttt ttgataatct gtttattacg
     8821 aactatattt ttaaaaataa aacttaagtt cgtttttagg aggattgttt gaaaaattta
     8881 aatcgttatg tagttgcggt ttctggagtc gttttacatt taatgctagg atcaacttat
     8941 gcttggagtg tgtttcgtaa cccaatcatc tcagagactg gttgggatat ttcatcagtt
     9001 tcattcgctt ttagtttggc tattttttgt ctaggaatgt ctgcagcttt tatgggacac
     9061 ttagtagagc gttttggtcc taggataatg ggaatgattt ctgctatttt atatggagca
     9121 gggaatgtgt taacaggttt agccattgaa actcagcagt tatggttact gtatgttgca
     9181 tacggtattt taggaggaat cggacttggt tcaggttata ttactccagt atcgactatt
     9241 attaaatggt ttcctgatag gaggggacta gcaacaggat tcgctattat gggatttggc
     9301 tttgcttctt tagtaacaag tccgcttgca caatccttaa tgattaggat tggtgtaggt
     9361 aaaacatttt atattttggg attagtatat ttttttgtca tgatgattgc ctcacaattt
     9421 attaaacaac cacctcagga aaaaataact attttgactc acgacggtaa aaagaatgct
     9481 atgaattcac aaattattac tggattaaaa gcaaatgctg ctataaaatc aaaaaccttt
     9541 tacatcattt ggttgacttt gtttattaat atttcgtgtg gcttaggttt aatatcagca
     9601 gcttcaccaa tggcacaaga tttagcaggc tattccgcag aatctgcagc cttattagta
     9661 ggggtactag ggatatttaa cggttttgga cgtctgttat gggcaagtct ctctgactac
     9721 attggacgcc cgttgacctt tataatatta tttattgtga actttattat gacttctagt
     9781 ttatttttgt cattcaatgc tattgtattt gcaatagcaa tgtctatttt aatgacttgt
     9841 tatggtgcag gcttttcctt attacctgct tatctaagtg atatttttgg aacaaaggaa
     9901 ttagctactt tacatggtta tagtttaaca gcatgggcaa tagcaggtct gtttgggccc
     9961 ctattgttat caaagacata ttcatgggga aattcctatc aattgacatt aatggttttt
    10021 ggttttttat tcttattcgg attattgtta tctctatatt taagaaaatt aacaactaaa
    10081 gttgtgtagt atatatctaa tttaaaattc tttggtttta ccaaagagtt ttttctttga
    10141 aaaatagttt tgtcttatag caaataattt gatatactaa gtatctagta aaaaaatgat
    10201 agaaagataa tactaaaatg atagaattct tttctaatat cagaacagag attccgcaga
    10261 tgcctttact tatccatagt ttgattttat ctgtcttacc ttttctgatg tggctgactt
    10321 tggttaatag agataagcct ttgtataaaa ctatttggag tatcctttta ggacttcagt
    10381 taattacgat ttatacttgg tttttctggg caaaattgcc tttatctgaa agtcttcccc
    10441 tttaccattg tcgaataggc atgtttgtcg ttctcttggc gaggcctgga atattaaagg
    10501 attatttcgc attgttagga gtagtaggtg gagtattggc aatgattcac ccagactttt
    10561 acccttacca gtttctacat gtgactaata ttttcttctt tattggacat tttgctttat
    10621 ttgtccttag tttactacat ctgatgacac agtccaattt ggacaaatta aatccaaaat
    10681 tgattataca actaacgctt ctcattaata tgtctttgat ttttattaac ctcttgactg
    10741 gggggaatta tggctttatg atgaagacac caatactagg aataacaaat ccctttttaa
    10801 acctttttat agtgacaact ctattaagtt ttctcgtcct atttgttaaa caaattttcc
    10861 aaaaagtgag aatgagagct agtcttaaaa cagaagaatc ttaattattt taatattatt
    10921 atattatcta gctattaaaa aataaatagc tggataatgt ttttcatttt tagctatttt
    10981 tatgattgac cgctaattat taatctgcta agatatagta cgtaataaaa acttaaccgg
    11041 ttaattattt ttgaaatata ttttttctgg aggaaaatag taatgaataa atcattcaat
    11101 accaaattag gtttggtaac tgtagcggta ttatcaggta ttgtattaac gtctcaatta
    11161 cctgtgaacg ctaaagctga gacaccagta atggcagcaa gtgcacaaca aggtttccgt
    11221 tttgtagcaa ccgttgtaga tagtcagacg catgtcttgc cgggaaaatt ggtaactttg
    11281 tcagaggtga caagtggtca gcctaaaatt attgcaagtg tgaaaacgaa tgatgccgga
    11341 caagctattt ttaataatct ccctataaaa cgtaacctta gtgtgtctgt tgatggacaa
    11401 gtaaaaggat atactattcg aactgacgta gcaggttcta gtaaagcagc tagttttaca
    11461 gcaacaggag tagggacaaa tgagccaacg tattcaaaaa aaaccataga catcacagtg
    11521 agagaccaga atgctgaacc tgtttcaggc aggacagtta ccttaaaaac acaagctggt
    11581 cgtgagattg ctagcttggt ttctggtgac aatggattaa ctcgctttac tgatcgttta
    11641 ctagatggaa cattctacca atattttgtt gatgggaaaa aaattggtga tattgtacct
    11701 ggagaatcaa gaagtgcata tgttaatgtt gcacctaaaa aagattattt tacctttaca
    11761 gtcactgctt tagataaaga tggacttgtt gttaagggaa aagaagtaac gttgactgat
    11821 attactgatg gtaaagcagt agcgcttgct tctttaaaaa cgaatgataa tggacaagct
    11881 atctttacca acctaccatt atcaagaaat atcagtgttt caattgatgg aaaatcaaag
    11941 ggctatactc ttcgaactga tgtagcaggc tctcataaag cggctgcatt ctatgtagat
    12001 ggtaaaggta ccaaagcacc tcaatttagt gcagaagcag cggttgtaac tgtttacgat
    12061 gctaatggaa atactatagc aaaccaagaa gtgacattaa ctaattcaaa tgggacaatt
    12121 gttgcaaaag gtttgactga aaaagatggc aaagcaaggt tcgccaataa attaatggca
    12181 ggaacgttat acaatatttt tgtaaatggt attgagatgc ctaaaactgc ccttgttggt
    12241 agtgatgtca gtgtttttct aacagataaa caaattaaga aagaaaaacc tgttactcct
    12301 gataaattgg ataaagaaat gaaccaagag aaggaaaagg agctcttaga tactccaaaa
    12361 ccagtaccac ctacttcgaa accaacagca ccaattaaaa ataaaaagga cgattcccct
    12421 aagaagttca cgaaaagtca atcttctagt ttgaagtcaa gtgttaaggc tataggtgct
    12481 aagccaaaag ctatgacagc cttagctaaa aaattgccta aaactggtga tcaagccatt
    12541 agtattttaa ctttggttgg ttttattgta acagggttag cgattatgtt aggcttcctt
    12601 aaaaaatcac gatcaaaata atttatttta gaagcttggg tctttagaca caggcttctc
    12661 ttctttcttc ggaaatagat tgaacttgaa gaatgttgtt aaattcgcta taataataaa
    12721 gttatagaca agattattgt ttcagttgag actactgaaa ataatactaa gaaatggata
    12781 aaagctaagg attgaatatg acattacaag acgaaatcaa aaaacgccgt acttttgcca
    12841 tcatctctca cccggatgct ggtaagacga ctattactga gcaattatta tattttggtg
    12901 gtgaaattag agaagcaggg acagtaaaag ggaaaaaatc aggtactttt gcaaagtccg
    12961 actggatgga tattgaaaag caacggggta tctctgttac ttcatctgtt atgcaatttg
    13021 attacgcggg taaacgtgtt aatattttgg atacacctgg acatgaggat ttctcggaag
    13081 atacataccg gaccttaatg gctgtggatg cagctgtcat ggttgtagat tcggctaaag
    13141 gtattgaggc tcaaactaaa aaactttttg aagttgttaa gcatcgtaat attccggttt
    13201 ttacgtttat taataaattg gaccgtgatg gacgtgagcc attggatttg cttgaagagt
    13261 tagaagaagt actaggtatt gcttcttatc caatgaattg gccgattggt atggggaaat
    13321 catttgaagg gctttatgat ttacataata aacgtttaga actctataag ggagacgagc
    13381 gttttgcaag tattgaagac ggtgatcagc tgtttgccaa taatccattt tatgaacaag
    13441 tcaaagaaga tattgaactt cttcaagaag caggtaatga cttttcagaa caagctattc
    13501 ttgatggtga cctaactcca gtattctttg gctctgccct tacgaacttt ggagttcaga
    13561 cttttcttga tactttcttg gagtttgctc cagaacctca tggtcacaag actacagaag
    13621 gtaatgttat tgaccccttg gctaaagatt tttcaggttt tgtctttaaa attcaagcta
    13681 acatggaccc acgtcatcgc gaccgtattg cttttgttcg tattgtttct ggtgaatttg
    13741 agcgcggtat gggagttaat ttgacacgta ctggtaaagg tgctaagctc tccaatgtta
    13801 ctcaatttat ggcagaatca cgtgaaaatg tgacgaatgc tgttgctggg gatattatcg
    13861 gggtttatga tacaggaact tatcaagttg gggatacctt aacagttggg aaaaataaat
    13921 ttgaatttga accattacca acttttaccc cagaattatt tatgaaagta tcagcaaaaa
    13981 atgttatgaa gcagaaatca ttccacaaag gtattgagca attagtacaa gaaggagcaa
    14041 tccagcttta taagaattat cagacaggcg aatacatgct gggtgctgtg ggtcaacttc
    14101 agtttgaagt ttttaaacat cgtatggaag gtgaatacaa tgctgaagtt gttatgacac
    14161 caatgggtaa aaagactgta cgttggatta actcagacga tttagacgaa cgcatgtctt
    14221 caagccgaaa tattttagca aaagatcgtt ttgaccaacc agttttccta tttgaaaatg
    14281 attttgcact tcgctggttt gctgataagt atcctgatgt gaagcttgaa gaaaaaatgt
    14341 aaaatttaaa gtcactcaaa tttgagtgac tttttaagtt ttttagacta aaatagttat
    14401 aatacataga aaagattgga gattaacatg ggaatttttt cgggattaat gggaaatgct
    14461 tcacaaatgg ataccgataa agtcgagaat cagttatctg atattctgat ttcagatgaa
    14521 caggttgatt tagcttatac cttaattcgt gatttgattg tttttactaa ttatcgttta
    14581 attttagtcg ataaacaggg ggtgacaggt aaaaaggttt cttataattc tataccttat
    14641 gcttctatat cacgttttac agtggaaact agtggtcatt ttgatttaga tgctgagtta
    14701 aagatttgga tatcatcagc aatagaacca gcagaagtac ttcagtttaa gaacgaccgt
    14761 aacatagtga gtatacagaa agctttggcg acagctgttt taaaataaac aatttagcag
    14821 tagtgatttt tatcactgct tttattttga agtatagtag gcctcaagca tatattttga
    14881 aaacgctgta tttttttgat aagatat
//
LOCUS       DATRNK010000050        14873 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.050, whole genome shotgun sequence.
ACCESSION   DATRNK010000050 DATRNK010000000
VERSION     DATRNK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14873
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.050"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..162)
                     /locus_tag="VBC52_001509"
     CDS             complement(<1..162)
                     /locus_tag="VBC52_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883089.1"
                     /translation="MLKTRTHINELETKIALDLVHRYLGIQQLVFSKWRHSLETGSWS
                     SIITINGANI"
     gene            complement(288..3470)
                     /gene="carB"
                     /locus_tag="VBC52_001510"
     CDS             complement(288..3470)
                     /gene="carB"
                     /locus_tag="VBC52_001510"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEO6883090.1"
                     /translation="MPKRTDIRKIMVIGSGPIVIGQAAEFDYSGTQACLSLKEEGYQV
                     VLVNSNPATIMTDKDIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMAL
                     SKNGILEELNVELLGTKLSAIDKAEDRDLFKQLMEELNQPIPESEIVNSVEEAIQFAE
                     QIGYPLIVRPAFTLGGTGGGMCDNQEQLVDITTKGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDAADNALVVCNMENFDPVGIHTGDSIVFAPAQTLSDVENQLLRDASLDIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPITKTTYAMFEPALDYVVAKMPRFPFDKFESGDRKLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVDHIKIADLDNVSDDVLLEKIRNAEDDRLFYLAETLRRHYSIEKLAS
                     LTSIDSFFLDKLRVIVELEDLLSKNRLDINILKKVKNKGFSDKAIASLWQINEDQVRN
                     MRKEAGILPVYKMVDTCAAEFDSATPYFYSTYAVENESLISDKASILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIRESGFEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIDL
                     EKPEGVILQFGGQTAINLAKDLNKAGVKILGTQLEDLDRAENRKQFEATLQALNIPQP
                     PGFTATTEEEAVNAAQKIGYPVLVRPSYVLGGRAMKIVENEEDLRHYMTTAVKASPDH
                     PVLIDAYLIGKECEVDAISDGQNILIPGIMEHIERAGVHSGDSMAVYPPQTLSETIIE
                     TIVDYTKRLAIGLNCIGMMNIQFVIKDQKVYVIEVNPRASRTLPFLSKVTHIPMAQVA
                     TKVILGDKLCNFTYGYDLYPASDMVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     INLQKALYKAFEAAYLHMPDYGNIVFTVDDTDKEEALELAKVYQSIGYRIYATQGTAI
                     YFDANGLETVLVGKLGENDRNHIPDLIKNGKIQAVINTVGQNNIDNHDALIIRRSAIE
                     QGVPLFTSLDTAHAMFKVLESRAFTLKVLD"
     gene            complement(3501..4577)
                     /gene="carA"
                     /locus_tag="VBC52_001511"
     CDS             complement(3501..4577)
                     /gene="carA"
                     /locus_tag="VBC52_001511"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEO6883091.1"
                     /translation="MKRLLLLEDGSVFEGEAFGADVETSGEIVFSTGMTGYQESITDQ
                     SYNGQIITFTYPLIGNYGINRDDYESIRPTCKGVVVYEWAEYPSNWRQQMTLDEFLKL
                     KGIPGISGIDTRALTKIIRKHGTMKACLINEGNSIHEALENLQKSVLLNNQIEQVSTK
                     LAYASPGVGKNIVLVDFGLKHSILRELSQRQCHITVVPHTTTAQEILNLNPDGVLLSN
                     GPGNPEQLPNALQMIQEIQSKIPIFGICMGHQLFAKANGAKTYKMTFGHRGFNHAVRH
                     LQTGRVDFTSQNHGYAVSREDFPEDLFITHEEINDKTVEGVRHKYYPAFSVQFHPDAA
                     PGPHDTSYLFDEFINMIDDFQQKS"
     gene            complement(4591..5514)
                     /locus_tag="VBC52_001512"
     CDS             complement(4591..5514)
                     /locus_tag="VBC52_001512"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEO6883092.1"
                     /translation="MNHQTQTLSLEHFVSLEELSNQEVMSLIKRSIEVKENPSNIGFD
                     KDYYVSNLFFENSTRTHKSFEMAELKLGLKTIEFNADTRSVNKGETLYDTILTMSALG
                     LDVCVIRHPDIDYYKELIASPNIHSAIVNGGDGSGQHPSQSLLDLVTIYEEFGYFKGL
                     KIAIVGDLTHSRVAKSNMQVLKRLGAEIFFSGPKEWYSSQFDEYGQYLPIDQLVDQID
                     VLMLLRVQHERHDGKGAFSKESYHQQFGLTKERYKHLRDTAIIMHPAPVNRDVEIASD
                     LVEADKARIVKQMSNGVYARIAILEAVLNSR"
     gene            complement(5679..6971)
                     /locus_tag="VBC52_001513"
     CDS             complement(5679..6971)
                     /locus_tag="VBC52_001513"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEO6883093.1"
                     /translation="MYIIKNGLIIDPQSGFNQVSDMLIDQGKIKQISKEIDIKGIPII
                     DASNKIVAPGLVDIHVHFREPGQTHKENIHTGALSAAAGGFTTVLMMANTNPTISSPE
                     IVKQVKESAAKEAIKIETVATITKSLNGKDLVNFEELLEAGAAGFSDDGIPLTDTKVL
                     QEAMNLARKHDVVLSLHEEDPSLNGVLGINEHIAQKIYHVCGASGLAEYSMIARDAMI
                     AYQTQAKVHIQHLSSSESVEVVDFAQKLGANLTAEVTPQHFSKTEDLLLTKGANAKLN
                     PPLRLEKDRQALIDGLKSGVISIIASDHAPHHIMEKAADNISQAPSGMTGLETSLALG
                     ITYLVSTKELSMIDFLAKMTCNPAQLYGFDAGYLREGGPADIVIFDQAEERIIKAEFA
                     SKSSNSPFIGDKLKGVVHYTICNGEIVYQKDSHAATLV"
     gene            complement(6983..7612)
                     /locus_tag="VBC52_001514"
     CDS             complement(6983..7612)
                     /locus_tag="VBC52_001514"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEO6883094.1"
                     /translation="MDLARQIAMELLDIQAVYLRPKQPFTWASGVKSPIYTDNRVTLS
                     YPETRTLIENGFVKQIQEHFPNVDIIAGTATAGIPHGAIIADKMNLPFAYIRSKAKDH
                     GVGNQIEGRVYSGQKMVIIEDLISTGGSVLEAVTAAQSQGIEVLGVVAIFTYQLAKAE
                     QAFREADIPLVTLTDYNQLIKVAKVNGYITADQLVLLKKFKEDQMNWQS"
     gene            complement(7625..8326)
                     /gene="pyrF"
                     /locus_tag="VBC52_001515"
     CDS             complement(7625..8326)
                     /gene="pyrF"
                     /locus_tag="VBC52_001515"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEO6883095.1"
                     /translation="MLEKCPIIALDFSDLASVITFLEHFPKEELLFVKIGMELYYSEG
                     PSIIRYIKSLGHRIFLDLKLHDIPNTVRSSMSVLAKLGIDMTNVHAAGGVEMMKAARE
                     GLGEGPILLAVTQLTSTSQEQMQVDQHINLSVVDSVCHYAQKAQEAGLDGVVTSAQEV
                     KQIKKQTNEHFICLTPGIRPPQTNQLDDQKRTMTPEQARIVGADYIVVGRPITKAENP
                     YQAYLDIKEEWNRIK"
     gene            complement(8539..9771)
                     /locus_tag="VBC52_001516"
     CDS             complement(8539..9771)
                     /locus_tag="VBC52_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEO6883096.1"
                     /translation="MQNDMSVVLRAGKLLIESGAEVYRVEDTMKHFAKALQIENFEAY
                     VVSSSIIASGINRYGKQEAKVCNTDGVTANLGRLEAVNNLSRQIAKQDLVSPEEIVKQ
                     LDLIEHQKDYSLLVTLISYFCGAGSFSLALGSSLLDSFSAAVTGLILGYFLNLMESRI
                     HTGFLLTILGSSVVALSANLLYFSGLGEHRSIIILGALMVMVPGAAFVNSVREFSQNN
                     FSTGLALIMSALLICISISAGVAITIEIIPFAEQMTGSFSGVPNTILEITIRTLMAGL
                     GTIAFSILYHVPKRYFLDLGILGSISWMLYLILWQQFHMDAIAVFFPGLFITYFSRLL
                     AAKRKCPATVFLATSMFPLIPGLSFYRAVYFLLTGADALAMEYFRSCFVTAFTIAIAI
                     SIVQQIPLSFFIRRKMIK"
     gene            complement(9811..11352)
                     /locus_tag="VBC52_001517"
     CDS             complement(9811..11352)
                     /locus_tag="VBC52_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6883097.1"
                     /translation="MSILEVKNLSHGFGDRAIFENVSFRLLKGEHIGLIGANGEGKST
                     FMSIVTGHLQPDEGKIEWSKYVTAGYLDQHTVLEEGQTVRDVLRTAFDELFKTEARIN
                     DIYISMADDGADVDSLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDSDVT
                     ELSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQDLVRYSGDYTNFESVYAMKKAQLEAAYERQQKEIADLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPSFEFKESRTPGRFIFQAKDLQIGY
                     DRALTKPLNLTFERNQKIAIVGANGIGKTTLLKSLLGIIPPISGNVERGDFIDLGYFE
                     QEVPGGNRQTPLEAVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQSKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKAYKGSILMVCHEPDFYEGWMDDVWD
                     FNQLS"
     gene            complement(11483..11821)
                     /locus_tag="VBC52_001518"
     CDS             complement(11483..11821)
                     /locus_tag="VBC52_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883098.1"
                     /translation="MQIIKRDGQIVEFDPEKIYQAILKAAQTVYVLDESLRQDLAQVT
                     KKVILDLEDAQVERPTINMIQSLVENRLLDAGYIHIAEHYISYRLQRDLERNGYDDQI
                     TVHLHFEKIK"
     gene            complement(11991..13067)
                     /locus_tag="VBC52_001519"
     CDS             complement(11991..13067)
                     /locus_tag="VBC52_001519"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEO6883099.1"
                     /translation="MGYTVAIVGATGAVGTQMIRQLEQSNLPIEQVKLLSSSRSAGKI
                     LHFKDEAIRVEETTKESFYDVDIALFSAGGSISAKFAPYAVKSGAVVVDNTSYFRQNP
                     DVPLVVPEVNAHAMTGHNGIIACPNCSTIQMMIALEPIRQKWGIERVIVSTYQAVSGS
                     GARAVEETKEQLRQVLNDNLSPDQLIATVLPCSSDQKHYPIAFNALPQIDTFTDNDYT
                     YEEMKMTLETKKIMEDATIKVSATCVRIPVLSGHSESIYIETKELASISEIKKAIANF
                     PGAVLQDLPSQQIYPQAINAVGHRETFVGRIRKDLDQENGVHMWVVSDNLLKGAAWNS
                     VQIAETLHKNGLVKPAKELKFELI"
     gene            complement(13276..14652)
                     /locus_tag="VBC52_001520"
     CDS             complement(13276..14652)
                     /locus_tag="VBC52_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6883100.1"
                     /translation="MFNKIGFRTWKSGKLWLYMGVLGSTIILGSSPVSAMDSVGNQSQ
                     GNVLERRQRDAENRSQGNVLERRQRDAENRSQGNVLERRQRDAENRSQGNVLERRQRD
                     VENKSQGNVLERRQRDVENKSQGNVLERRQRDAENKSQGNVLERRQRDAENKSQGNVL
                     ERRQRDAENRSQGNVLERRQRDVENKSQGNVLERRQRDVENKSQGNVLERRQRDADNK
                     SQGNVLERRQRDVENKSQGNVLERRQNNVLIKSQDNVLERRQRDADNKSQGNVLERRQ
                     RDADNKSQGNVLERRQRDVENKSQGNVLERRQNNVLIKSQDNVLERRQRDADNKSQGN
                     VLERRQRDVENKSQGNVLERRQHDVENKSQVGQLIGKNPLFSKSTVSRENNHSSQGDS
                     NKQSFSKKVSQVTNVANRPMLTNNSRTISVINKLPKTGDDQNVIFKLVGFGLILLTSL
                     CGLRRNEN"
BASE COUNT         4518 a         2991 c         2395 g         4969 t
ORIGIN      
        1 aatattagcg ccatttattg ttataataga agaccatgaa ccagtttcta aagaatgtct
       61 ccactttgaa aaaacaagtt gttgaatacc tagatatctg tgtactaaat caagggcgat
      121 ttttgtttct aattcattaa tatgtgttcg tgtttttaac ataattaact ccttaagttt
      181 gttccctatt ttatcacaat taggcttttt tataaaaaca gaacaaacta tatttaatct
      241 agtccgattc tgttttgctg gaagttaatt ttaccttgac cgtttgatta atctagtact
      301 tttaatgtaa atgctctgct ttcaagaact ttgaacattg catgtgcagt atctaaagat
      361 gtaaatagag gaactccttg ttcaattgca gaacgtctga tgatgagagc atcatggtta
      421 tcaatgttat tttgcccaac tgtgttgata actgcttgaa tcttaccatt tttaattaaa
      481 tcaggaatat gatttcgatc gttctctcct aacttaccta ctaaaactgt ctctaaacca
      541 ttagcatcaa aataaattgc agtcccttgg gtagcataaa tacgataacc aatactttga
      601 tagacttttg caagttctaa agcttcttct ttgtctgtat catctactgt aaaaacaatg
      661 ttaccatagt ctggcatatg gagatatgct gcttcaaagg ctttatacaa ggctttctga
      721 agattaatat ctgatcccat cacttcgcct gttgatttca tttcaggacc taataaacta
      781 tctactttag caagtttggt aaaactaaag acaggggctt taatgtggac catatctgaa
      841 gctggataga gatcataacc atatgtaaaa ttgcagagtt tatctcctaa gataactttt
      901 gttgctactt gggccatagg aatgtgagtt acttttgata agaatggaag tgttctacta
      961 gcacgaggat taacttcaat aacgtatact ttctgatcct tgattacaaa ctgaatattc
     1021 atcataccga tacagtttaa tccaattgct aagcgttttg tataatctac aatagtttcg
     1081 ataatggtct cagacaatgt ttgaggaggg tatacagcca tggaatctcc tgaatgaaca
     1141 ccagctcgct ctatatgttc cattattcca ggaatcagta tattctgacc atcggatata
     1201 gcatcaacct cacactcttt acctataagg tatgcatcta taagcactgg atgatctggg
     1261 cttgccttta ccgcggttgt catataatga cgtaaatctt cttcgttttc aactatcttc
     1321 atagctcgtc caccaagaac atatgatggt ctaaccaaca cgggataacc aatcttttgc
     1381 gctgcattaa ctgcctcttc ctccgtagtg gcggtaaaac ctggtggttg agggatattt
     1441 agggcttgca aagtggcttc aaattgtttt ctattttcag cacgatccaa atcttctaat
     1501 tgtgtgccta gtattttaac ccctgccttg tttaaatctt ttgctagatt aatagcagtc
     1561 tgaccaccga attgtaagat gaccccttca ggtttttcta agtcaataac attcataaca
     1621 tcttcaaaag tcaaaggttc aaagtaaagc ttatctgaaa tagaaaaatc tgttgagaca
     1681 gtctctggat tagagttcat gataatcgct tcaaaaccag actctctgat agctttaaca
     1741 gaatgaaccg ttgcataatc aaattcaacg ccctgtccaa ttcggattgg gcccgatcct
     1801 aaaaccaaaa tagaggcttt atctgatatc aatgactcgt tttctacggc ataagttgaa
     1861 taaaagtagg gtgttgctga atcaaactca gccgcacatg tatcaaccat cttataaact
     1921 ggaagaattc ctgcttcttt tcgcatgtta cgaacttgat cttcgtttat ttgccataaa
     1981 ctcgctatag ctttatctga aaaaccttta tttttaacct ttttgagaat attgatatcc
     2041 agtctgtttt tagataataa atcttctaac tctacaatca ctcttaactt atcaaggaag
     2101 aaagaatcaa tactagtcaa acttgctaat ttttcaatac tataatgtct acgtaaagtt
     2161 tctgctagat aaaataagcg atcatcttct gcatttctta ttttttctaa taaaacatca
     2221 tctgatacat tatctaaatc tgctatttta atgtgatcga cacctatttc tagtgaacga
     2281 caagctttca acaaagattc ttctatattc cgtcctattg ccatgacttc gcctgtagct
     2341 ttcatctgtg ttcccaattt tctatcgcca ctttcaaatt tatcaaaagg aaatctcggc
     2401 atttttgcga ctacgtaatc taaagcaggt tcaaacatag cgtaagtcgt ttttgtaata
     2461 ggattaatta cctcatctag tgtcaaacca acagctatct tagctgctaa tttagcaatt
     2521 ggatatccag tagcttttga agctaaagca gaagatcttg atactcttgg attaacttca
     2581 atgacatagt atttaaaact attcggatca agggctagtt gaacattgca tcctccttca
     2641 attttcaaag ctcttataat atctaaactc gcatctcgta ataactgatt ttctacatca
     2701 gataacgttt gtgcgggtgc aaaaactata gagtcccctg tatggatacc aacaggatca
     2761 aaattctcca tattacaaac aacaagagcg ttatctgctg catcacgcat aacttcgtat
     2821 tcaatctctt taaagccagc aattgaacgt tcaataaggc actgtgtcac aggcgataac
     2881 tttaacccct tcgtcgtgat gtcaaccaat tgttcttgat tatcgcacat gcctccacct
     2941 gtacctccta aagtaaaggc aggacgaaca attaatggat acccgatttg ctctgcaaat
     3001 tgaatagctt cttctaccga attaacaatc tcggattctg gaataggttg attgagttct
     3061 tccatcaact gtttaaataa atcacgatct tcagctttat caatggctga taatttggtc
     3121 cctaatagtt caacatttaa ttcttctagg ataccatttt ttgatagagc catagccata
     3181 ttcaatccag tttgacctcc aagagttgga aggagagcat caggtctttc cttcctgaga
     3241 attcttgtga caaactccaa ggtaataggt tcaatataaa ctttatcagc aatatcttta
     3301 tctgtcatga tagtagctgg attcgaattg accaaaacaa cttggtatcc ctcctctttt
     3361 aaagagagac aggcttgtgt accagaatag tcaaactctg ctgcttgtcc aatcacaatc
     3421 gggccagaac cgataaccat aattttacgt atgtctgtgc gctttggcat atcaaactcc
     3481 tctaattcta tattttaaag ctagcttttt tgctggaaat catcaatcat attaataaat
     3541 tcatcaaaaa gataagatgt atcatgcggc cctggtgctg catctgggtg aaactgcact
     3601 gaaaaggcag gatagtattt atgtcgaaca ccttcgacgg ttttatcatt aatctcttca
     3661 tgtgtaatga agagatcctc agggaaatct tctctcgaga cagcataacc atgattttga
     3721 cttgtaaaat caacccgtcc tgtttgcaaa tgacgaacag catgattaaa acctcgatga
     3781 ccaaaagtca ttttataagt ctttgcacca ttggctttag caaatagttg atgtcccata
     3841 caaataccaa aaattggaat tttgctttga atttcttgga tcatttgtaa agcattcggt
     3901 aattgttctg ggttcccagg gccgttagat aagagtactc catctggatt gagatttaag
     3961 atttcttgcg ctgtcgtagt atgagggacc actgtaatat gacattgacg ttgtgacagt
     4021 tctcttagaa tagaatgttt taaaccaaag tcaactaata caatattttt gccaactcca
     4081 ggcgaagcat aagctaattt ggttgatact tgttcaattt gattatttaa aagaacactt
     4141 ttttgaaggt tttctagtgc ttcatgaata gaatttccct cgtttattaa acaagctttc
     4201 attgttccat gttttcgaat aatttttgtt aatgctcgag tatctatacc tgaaattcca
     4261 ggtattcctt ttaattttaa aaattcatca agtgtcatct gttgacgcca gttacttgga
     4321 tactctgccc attcataaac aactactccc ttacatgttg gtctaataga ttcgtaatcg
     4381 tctcgattta tcccataatt tccaataaga ggataagtaa aagtgatgat ttgaccgttg
     4441 taagattgat ctgttataga ttcttgatac cctgtcattc ctgtactaaa aacgatttca
     4501 ccacttgttt ctacatccgc tccgaaagcc tcaccctcaa acacactacc atcttccagt
     4561 aataatagac gcttcatttt atctccttta ttatctgcta ttgagcactg cttctaagat
     4621 agctatcctt gcatagacgc cattactcat ctgtttcact attcgagcct tatctgcttc
     4681 tactaaatcg ctagcaatct ctacatccct attgacgggt gctgggtgca taataattgc
     4741 tgtatccctt aaatgtttat agcgttcttt tgtaagacca aattgttgat gatagctttc
     4801 ttttgaaaac gcccctttac catcatgacg ttcgtgttga acacgtaaaa gcataaggac
     4861 atcaatttga tcaactaact gatctattgg taaatattgc ccatattcat cgaactggct
     4921 actataccat tctttgggtc cagaaaagaa aatttcagct cctaatcgtt ttagcacttg
     4981 catgttagat ttagctaccc gtgagtgagt taaatctcct acaatcgcaa tttttagccc
     5041 tttgaagtat ccaaattctt cgtaaatagt taccaaatcc aaaagtgatt ggcttggatg
     5101 ttggcctgag ccatcacccc cattgacaat agctgaatga atgttcggac tagcaatcaa
     5161 ttctttataa tagtcaatat caggatgcct aatgacacaa acgtccaatc ctagagcact
     5221 cattgttaag atagtgtcat aaagagtttc tcctttatta acagatcttg tatcagcatt
     5281 aaattcaatt gtttttaatc ctaatttgag ttctgccatt tcaaatgact tatgtgttct
     5341 tgttgaattc tcaaaaaaga gatttgacac atagtaatct ttatcaaaac caatgttgct
     5401 aggattttct ttaacttcga tacttctctt tatcaaagac ataacttctt gatttgataa
     5461 ttcttctaaa ctgacaaaat gttctaatga aagtgtttgt gtttgatgat tcatgataat
     5521 ttctttctat aaattttagc tagaaaaaag cctcctaaaa caaggaggct ctgcaaaata
     5581 gtaagctaga tagagaacta acttactatt tgttaacgca attccttgtt tgcctctctg
     5641 gacaatacct taaaggatat taataagaaa tgttttacct ataccaatgt cgctgcgtgg
     5701 ctatcttttt gatagactat ttcaccattg caaattgtgt agtgaacaac tcccttcaat
     5761 ttatcaccga taaaaggaga gttgctggat ttggaggcaa attctgcctt tattattctc
     5821 tcttcagctt gatcaaaaat aacgatatct gcaggtccac cttctctgag ataacctgca
     5881 tcaaatccat ataactgtgc aggattacat gtcatttttg ccaaaaagtc aatcattgag
     5941 agttctttag tagatactaa ataagtaatc cctaaggcta atgatgtctc taagccagtc
     6001 ataccagagg gcgcttgact aatattatct gctgctttct ccataatatg atggggagcg
     6061 tggtcacttg caataattga aataacacca cttttaagac catcaatgag ggcttgtcta
     6121 tccttttcaa gtcgcagtgg tggatttaat tttgcattag cacccttagt taaaagtaag
     6181 tcctctgtct ttgaaaaatg ttggggggtt acttctgctg ttaagttagc accaagcttc
     6241 tgcgcaaaat caactacttc tactgattct gaacttgaca aatgctggat atggactttc
     6301 gcctgtgttt ggtaagcaat catagcatct cgcgcaatca ttgaatattc agcaagacca
     6361 ctagctccac agacatgata aattttctga gcaatatgtt cgttgatacc aagaacacca
     6421 tttaacgatg gatcctcttc atgtaacgat aggacaacat catgttttct agctaagttc
     6481 atagcttctt gcaaaacctt agtatctgtc aaagggatac catcgtctga gaatccagca
     6541 gctccagctt ctaatagttc ttcgaaatta actaaatctt taccattcag ggatttagta
     6601 attgtagcaa ctgtttcaat cttaattgct tcttttgctg cactctcctt aacttgtttt
     6661 acaatttctg ggctagaaat cgttgggtta gtattggcca tcataagaac tgtcgtaaaa
     6721 ccacctgctg cagctgataa ggctcctgta tggatatttt ctttatgtgt ctgtccaggc
     6781 tcacgaaaat gtacatgaat atcgactaat cctggagcta caattttatt gcttgcatca
     6841 atgattggaa tgcctttaat atcaatttct ttcgatattt gtttaatttt tccttgatca
     6901 atcagcatgt ctgatacttg attaaaacca ctttgagggt cgataatcaa tccgttttta
     6961 atgatataca tatctgtcct ctctaacttt gccaattcat ttgatcttct ttaaattttt
     7021 tcagtaatac cagttggtca gctgtaatat atccattaac tttagcaact ttaattaatt
     7081 gattgtaatc cgtcaaagtc accaaaggaa tatcagcttc acgaaatgct tgttcagcct
     7141 tagctaattg atatgtaaat atcgctacta cccctaaaac ctcaatacct tgactctgcg
     7201 cagccgtaac agcctctaaa acagatcctc ctgttgaaat caaatcttca atgataacca
     7261 ttttttggcc tgagtaaaca cgtccctcga tttgattccc cacaccatga tcctttgcct
     7321 tcgagcgtat ataggcaaag ggcaagttca tcttatcagc tataatagcg ccatgtggta
     7381 tgcctgcagt cgctgtccct gcgatgatat caacgtttgg gaaatgttcc tgtatttgtt
     7441 taacaaaacc attttcaatc agtgtccttg tctcaggata cgataaggtt acacgattat
     7501 cagtgtaaat tggcgatttg acccccgaag cccaagtaaa gggttgtttg ggtcttaagt
     7561 aaacagcttg aatatctaat agttccatgg caatctgtct tgctaaatcc atctcagtac
     7621 tcctttattt aatacgattc cattcttctt ttatgtctag atatgcttga tatggatttt
     7681 ctgctttcgt tataggtctt cccacaacga tataatcagc cccaactata cgtgcttgct
     7741 caggagtcat cgttcttttt tgatcatcta actgattggt ttgtggaggt ctgataccag
     7801 gcgttaagca aatgaaatgt tcgttagtct gtttcttgat ttgctttacc tcttgagccg
     7861 atgtgactac tccatctaag cctgcttctt gagctttttg agcataatgg caaaccgaat
     7921 caactacact taagttaata tgttgatcca cctgcatttg ttcttgtgaa gtcgaggtta
     7981 attgtgtcac tgctaatagt attggaccct cccccaaacc ttctctagct gctttcatca
     8041 tttcgactcc tcctgcagca tggacattag tcatatcaat gcctaatttg gctaagacag
     8101 acatagatga tcgaactgta ttagggatat catgtaattt taaatccaaa aaaatgcgat
     8161 gtcctaagga tttaatatat ctaataattg agggaccttc ggaataataa agctccattc
     8221 ctattttgac aaaaagcaat tcttcttttg gaaaatgttc taaaaaagtt attacagaag
     8281 ccaaatcaga aaaatctaag gcaatgatag ggcatttttc taacatataa ttcctctcta
     8341 tagaaataat aattgttgga aatgtaccag tatgacacaa aaaaccttgc ctgagggcaa
     8401 ggtttcataa aaagttagcg aaaaagcact cgctatttga cttatgatgg tgaccttgtt
     8461 cgcctctctg gactcactta aagatacatt tgtaggtatt ctacaagttt acaatcattt
     8521 tgtcaaataa atactaaatt attttatcat tttacgtcta ataaagaacg atagaggaat
     8581 ctgctgtaca atactaattg ctatagcaat tgtaaaagct gttacaaaac aggatctaaa
     8641 atattccatt gctaaggcat ctgctcccgt caataaaaag taaacagctc tatagaagct
     8701 aagtcctgga ataagaggaa acatacttgt cgctaaaaag acagtggcag gacacttacg
     8761 tttagcagct aatagccgtg aaaaataagt gatgaaaagc cctggaaaaa atacggctat
     8821 agcatccata tgaaattgtt gccataagat caggtagagc atccaagata ttgatcctag
     8881 tatacctaag tcaaggaaat aacgttttgg aacatggtag aggatagaga aggctatcgt
     8941 ccctaaaccg gccatcagtg ttctgatggt aatctcaagg atggtgttag gaacaccaga
     9001 aaaagaacct gtcatttgtt ctgcaaacgg aataatttca attgtaatag ccacgcctgc
     9061 tgatatagaa atacaaatga gtaaagcaga cataatcaaa gctaaacctg ttgaaaaatt
     9121 attctgtgaa aattctctca ccgaattaac aaaggcagcc cctggcacca tcaccattag
     9181 tgcccctaat atgataatgc tacgatgctc acctaatcct gaaaaatata ataaattagc
     9241 acttaaagcc actacagaac tacctagaat ggtcaaaaga aatcctgtat gtatacgact
     9301 ttccatcaaa ttcagaaaat atcctaaaat taagcctgtt acagcagcgg aaaatgaatc
     9361 taataaggaa ctacccaagg ctaaagaaaa actacccgct ccacaaaaat aagaaattaa
     9421 agtaacaagt aaactataat ctttttgatg ttcaattaag tctaactgct ttacaatttc
     9481 ttctggagat actaagtctt gtttagcaat ttgcctagaa aggttattaa cagcttcaag
     9541 tctacctaaa tttgctgtta caccatctgt attgcatact ttagcttctt gtttaccata
     9601 gcggttaatc ccggaagcga taatactgct actaaccaca taagcttcaa aattttctat
     9661 ctgaagagcc ttagcaaaat gcttcatcgt gtcttcgaca cgatagactt ctgctccgct
     9721 ttcaatcagt aatttacctg ctctaagaac aacgctcata tcattttgca tcttatttgg
     9781 ccctgccttc tactttatta ccctagccac ttaactaagt tggttaaagt cccaaacatc
     9841 atccatccat ccttcataaa agtccggttc atgacatacc atcaagattg atcccttata
     9901 agcttttaaa gcacgtttta actcatcttt agcatctaca tctaaatgat tggttggttc
     9961 atcaagtaca aggacattat tttcgcgatt cattaataag caaaatctga cctttgattg
    10021 ctccccacca gataacacct gaatctgact ttcaatatgc tttgaagtta aaccacaacg
    10081 agcaagagct gccctaacct ctgcttggtt caaagcagga aaagcatccc atactgcttc
    10141 aagtggagtt tggcgatttc ctccgggtac ttcttgttca aaataaccta aatcaataaa
    10201 atcacctcgt tcaacattac cagaaatagg ggggataata cctagtaaac tcttaagtaa
    10261 tgttgttttt cctatcccat ttgctccaac aatggctatt ttttgattac gttcaaatgt
    10321 taggtttaga ggttttgtta aagcacgatc atatccaatt tgaagatctt tagcttggaa
    10381 aataaaacgt cctggtgtgc gtgattcttt aaattcaaaa cttggctttg gtttctctgc
    10441 ttgtaactca ataatatcca ttttgtctag tttcttttga cgagacattg ccatattacg
    10501 tgttgcaacg cgtgctttat tacgattaac aaaatcttgt agatcagcta tttccttttg
    10561 ttgacgttca taggctgctt ctaattgagc ctttttcatt gcatagactg attcaaaatt
    10621 agtataatca ccggaatatc ttactaaatc ttgattttca acatgataga caatattgat
    10681 aacatcgttc aaaaatggaa tatcgtgtga gattaaaaca aaagcatttt cataattttg
    10741 taaatagcgt tttaaccaat caatgtgttc agcatcaagg tagtttgtag gctcatcaag
    10801 caggaggata tctggctttt ctaataacag ttttgctaaa aggacctttg tcctttgacc
    10861 accagataat tcagtaacat cagaatccat accaaaatcc ataactccta atgcccgtgc
    10921 gacttcatca attttagcat caagtgtata aaaatcacgt gattctaaac gatcttgtag
    10981 ttctccaact tcttccataa ggctatctac atctgcgcca tcatctgcca tggatatata
    11041 tatatcattg atacgtgcct ctgttttaaa aagttcatca aatgctgttc gcaaaacatc
    11101 acgaactgtt tgaccttctt ctaacacagt atgttgatca aggtaaccag cagttacata
    11161 ttttgaccat tcaatttttc cttcatcagg ttgaagatga cctgtaacaa ttgacatgaa
    11221 tgttgatttt ccttcaccat tagcaccaat aagtcctata tgttcacctt ttaaaaggcg
    11281 aaaagaaaca ttctcaaaaa tagcacgatc accaaaaccg tgacttaagt tttttacttc
    11341 taaaatactc atttaaaatc ctttgattga taatagaggt tattaaaaaa ctcatagaga
    11401 cactctacaa gtttttaaat tagtacatta catagtaagt tccatataca agtatagaga
    11461 gtttaaactt aaaagtcaaa ccttacttaa ttttttcaaa gtgtaaatga acagtgattt
    11521 gatcatcata accatttcgc tctaaatcac gttgcaaacg gtatgaaata tagtgttcag
    11581 caatatgaat gtatcctgca tccaataaac gattttcaac caaagactga atcatattga
    11641 tcgttggtcg ttcaacctga gcatcctcta aatctaagat tactttttta gtcacttgag
    11701 caaggtcttg acgaagtgac tcatcaagca cataaactgt ttgagcagct ttcaagatag
    11761 cttggtagat tttttcagga tcaaattcaa caatctgtcc atcacgtttg atgatttgca
    11821 taagagcccc tccttaatgt ggaaacaatg ttaaaacgat ttgcaatcat tttcatttgt
    11881 taatttcatt atgcaaatgt tttcataatt tgtcaagtat tttacctgat attgattttt
    11941 aatcaatatc actagcagtc agcataagta ttaagcaatg tcatattctt ctatattaat
    12001 tcaaatttta actctttcgc tggtttaacc agcccattct tatgcaatgt ttctgcgatt
    12061 tgaacagaat tccaagctgc ccccttcaat agattatctg aaactaccca catatgaaca
    12121 ccattttctt gatcaaggtc ttttcgaata cgaccaacaa aggtctccct atgacctaca
    12181 gcattaatcg cttgagggta aatttgctga gatggaaggt cttgtaggac ggcaccagga
    12241 aaattagcaa tagccttttt aatttcagag attgaggcta attctttcgt ctcaatataa
    12301 atggactcag aatgacccga taaaacggga atacggacgc aggtagctga aaccttaata
    12361 gtagcatctt ccataatttt ctttgtctcc aaagtcatct tcatttcttc gtacgtataa
    12421 tcattatcag taaaagtatc aatttgaggt aatgcattga atgcgatagg ataatgtttt
    12481 tgatcgctag aacatggcaa gactgttgca attaattgat caggtgaaag gttatcattt
    12541 aaaacttgtc tcaactgttc cttagtttct tcaacagcac gtgcacctga acccgaaaca
    12601 gcttgatagg tggaaactat aacacgctct atcccccatt tttgacgaat gggctctaaa
    12661 gcaatcatca tttgaatagt agaacaattg gggcaagcta tgataccatt atgaccagtc
    12721 atggcatgag catttacttc aggaacaact agtggaacat caggattctg acgaaaatat
    12781 gacgtgttat ctactacaac tgctccagac tttactgcat aaggagcgaa ttttgctgaa
    12841 atagatccac cagctgaaaa caaggcaata tcaacatcgt aaaatgattc tttagttgtc
    12901 tcttcaacac gtatagcctc atctttaaaa tgtaaaatct tacctgctga gcgacttgat
    12961 gataaaagtt tcacttgttc tattggtaaa ttcgattgtt ctaattgacg aatcatttgt
    13021 gttcctacgg cacctgtagc gcccacgata gcaacggtat atcccatata atgacctcta
    13081 tttgatgatg ttgttcatca atattttcaa aacattttaa tgtgactatt atactacatt
    13141 tttataaact ttcaagaata gccaaatgaa aaaagtcacc ctaaccaacc taattataaa
    13201 aaggaatctc gtattcgatt ccttttttat tgattgcata tatcattata atagttacta
    13261 aatggttgat tatacttaat tttcattgcg tctcaaaccg cagagacttg ttaataaaat
    13321 taaaccaaaa cctacaagtt taaaaatgac attttgatca tcacctgttt taggtaattt
    13381 atttatcact gaaattgttc tagaattatt agttaacatc ggtctattag ctacattagt
    13441 aacctgagat acttttttag agaatgactg tttgttagag tcaccttgac tagagtgatt
    13501 attttctcta gatacagttg actttgaaaa aagtggattt ttccctataa gttgacctac
    13561 ttgactctta ttctcaacat catgttggcg acgctctaaa acattacctt ggctcttatt
    13621 ctcaacatcg cgttgacgac gctctagaac gttaccctgg ctcttgttat ccgcatcacg
    13681 ttggcggcgc tctagaacat tatcttgact cttaataagg acattatttt ggcgacgctc
    13741 tagaacatta ccttggctct tgttctcaac atcacgttgg cgacgctcta gaacgttacc
    13801 ctggctcttg ttatccgcat cacgttggcg acgctctaaa acattgccct ggctcttgtt
    13861 atccgcatca cgttggcggc gctctagaac attatcttga ctcttaataa ggacattatt
    13921 ttggcgacgc tctagaacat taccttggct cttgttctca acatcacgtt ggcgacgctc
    13981 taaaacattg ccctggctct tgttatctgc atcacgttga cgacgctcta gaacgttacc
    14041 ttggctcttg ttctcaacgt cacgttggcg acgctctaga acgttacctt ggctcttgtt
    14101 ctcaacatcg cgttgacgac gttctaaaac attaccttgg cttctgtttt ctgcatcgcg
    14161 ttgacgacgc tctagaacat tgccttggct cttgttttcc gcatcacgtt gacgacgctc
    14221 tagaacattg ccttggctct tgttttccgc atcacgttga cgacgctcta gaacattacc
    14281 ttggctctta ttctcaacat cgcgttgacg acgctctaaa acattgcctt ggctcttatt
    14341 ctcaacatca cgttgacgac gctctagaac attaccttgg cttctgtttt ctgcatcacg
    14401 ttgacgacgc tctagaacat taccttggct tctgttttct gcatcacgtt gacgacgttc
    14461 taaaacatta ccttggcttc tgttttctgc atcgcgttga cgacgctcta aaacattgcc
    14521 ctgactttga tttccaacac tatccatagc agatacagga cttgatccta aaataatagt
    14581 tgatcctagc actcccatat aaagccaaag ctttcctgat ttccaagttc taaaacctat
    14641 tttattgaac aattaatttt ctccttactt tttttaaatt ttaaaagtca ttttattatt
    14701 acttttattt agattatata ttaaaacatt ttataagtta tgtcaagata atttattatt
    14761 tagttgcaat ttattattaa tttgtatgtt taaaattaaa tagttgatat ctaaaacatg
    14821 atatttttaa attttccttt aatattataa agcatgatag atagtcatac taa
//
LOCUS       DATRNK010000051        14436 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.051, whole genome shotgun sequence.
ACCESSION   DATRNK010000051 DATRNK010000000
VERSION     DATRNK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14436
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.051"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..196)
                     /locus_tag="VBC52_001521"
     CDS             complement(<1..196)
                     /locus_tag="VBC52_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6883101.1"
                     /translation="MVSSDNTNLKFLKAFSELLKMRSFEQIKVSDLAKKARLSRRSFY
                     NHYNSKEDFLRESILIIFDDI"
     gene            379..711
                     /locus_tag="VBC52_001522"
     CDS             379..711
                     /locus_tag="VBC52_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEO6883102.1"
                     /translation="MLRKYKQRNYNVAHTKQRFSIKKFKFGVASVLIGLTFLGMSSHS
                     VLADESSIMSTEVPQTAGVKPEVTVTQPTSDVTSLATDTSTSAVLSTDASHSNPTESA
                     EHAVGLEC"
     gene            complement(832..1975)
                     /locus_tag="VBC52_001523"
                     /pseudo
     CDS             complement(832..1975)
                     /locus_tag="VBC52_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2108..2245
                     /locus_tag="VBC52_001524"
     CDS             2108..2245
                     /locus_tag="VBC52_001524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883103.1"
                     /translation="MIIARENHQVTHEHVSILLTCVHELIVEVNQTQPLSREFREKYM
                     G"
     gene            2255..2510
                     /locus_tag="VBC52_001525"
                     /pseudo
     CDS             2255..2510
                     /locus_tag="VBC52_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
     gene            complement(2485..2980)
                     /locus_tag="VBC52_001526"
                     /pseudo
     CDS             complement(2485..2980)
                     /locus_tag="VBC52_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
     gene            complement(3174..4382)
                     /locus_tag="VBC52_001527"
     CDS             complement(3174..4382)
                     /locus_tag="VBC52_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO6883104.1"
                     /translation="MVTFDFKHVQTLHTISQLPISVMSRDKELVQLYGNEDYLLPYYQ
                     FLKHLAIPYNQDITVYEGLFEESFLIFPVCQYLIAIGPFYPYSPDINRQEQLSSRFLE
                     QFSHRSKKEILSYIKLVPCFPTTSIRSLLVSIDAFFQTQFEASCQQAINHLLEESEQI
                     VADPDIVLHLKHTKKNSFQLPTVLNHLNHIIDLVKLGNTQLLKQEINRLPSSSVTSSS
                     IPALRAEKNLTVVYLTKLLELSFEKNTDVAKSYALVKHYMALNEEAPDLIDVSRIRCA
                     ALIDFSESLTNKSISDKQQMYNSILHYVDNHLYSKLKVSDIANYLYISNSHLRSVFKK
                     YSDISLQSYILKAKIKEGQLLLQRGVPIGEVAKLLHFYDTTHFLKTFKKYVGISSNEY
                     LTKYRETSCQ"
     gene            4764..6428
                     /locus_tag="VBC52_001528"
     CDS             4764..6428
                     /locus_tag="VBC52_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO6883105.1"
                     /translation="MKLSKKLLFSAAVLTIVAGSTVEPVAQFATGMSIVRAAEVSQER
                     PAKTTVNIYKLQADSYKSEITSNGGIENKDGEVISNYAKLGDNVKGLQGVQFKRYKVK
                     TDISVDELKKLTTVEAADAKVGTILEEGVSLPQKTNAQGLVVDALDSKSNVRYLYVED
                     LKNSPSNITKAYAVPFVLELPVANSTGTGFLSEINIYPKNVVTDEPKTDKDVKKLGQD
                     DAGYTIGEEFKWFLKSTIPANLGDYEKFEITDKFADGLTYKSVGKIKIGSKTLNRDEH
                     YTIDEPTVDNQNTLKITFKPEKFKEIAELLKGMTLVKNQDALDKATANTDDAAFLEIP
                     VASTINEKAVLGKAIENTFELQYDHTPDKADNPKPSNPPRKPEVHTGGKRFVKKDSTE
                     TQTLGGAEFDLLASDGTAVKWTDALIKANTNKNYIAGEAVTGQPIKLKSHTDGTFEIK
                     GLAYAVDANAEGTAVTYKLKETKAPEGYVIPDKEIEFTVSQTSYNTKPTDITVDSADA
                     TPDTIKNNKRPSIPNTGGIGTAIFVAIGAAVMAFAVKGMKRRTKDN"
     gene            6517..7440
                     /locus_tag="VBC52_001529"
     CDS             6517..7440
                     /locus_tag="VBC52_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6883106.1"
                     /translation="MKQTLKLMFSFLLMLGTMFGISQTVLAQETHQLTIVHLEARDID
                     RPNPQLEIAPKEGTPIEGVLYQLYQLKSTEDGDLLAHWNSLTITELKKQAQQVFEATT
                     NQQGKATFNQLPDGIYYGLAVKAGEKNRNVSAFLVDLSEDKVIYPKIIWSTGELDLLK
                     VGVDGDTKKPLAGVVFELYEKNGRTPIRVKNGVHSQDIDAAKHLETDSSGHIRISGLI
                     HGDYVLKEIETQSGYQIGQAETAVTIEKSKTVTVTIENKKVPTPKVPSRGGLIPKTGE
                     QQAMALVIIGGILIALALRLLSKHRKHQNKD"
     gene            7442..8359
                     /gene="srtC1"
                     /locus_tag="VBC52_001530"
     CDS             7442..8359
                     /gene="srtC1"
                     /locus_tag="VBC52_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC1"
                     /protein_id="HEO6883107.1"
                     /translation="MGQKSKISLATNIRIWIFRLIFLAGFLVLAFPIVSQVMYFQASH
                     ANINAFKEAVTKIDRVEINRRLELAYAYNASIAGAKTNGEYPALKDPYSAEQKQAGVV
                     EYARMLEVKEQIGHVIIPRINQDIPIYAGSAEENLQRGVGHLEGTSLPVGGESTHAVL
                     TAHRGLPTAKLFTNLDKVTVGDRFYIEHIGGKIAYQVDQIKVIAPDQLEDLYVIQGED
                     HVTLLTCTPYMINSHRLLVRGKRIPYVEKTVQKDSKTFRQQQYLTYAMWVVVGLILLS
                     LLIWFKKTKQKKRRKNEKAASQNSHNNSK"
     gene            8316..9167
                     /gene="srtC2"
                     /locus_tag="VBC52_001531"
     CDS             8316..9167
                     /gene="srtC2"
                     /locus_tag="VBC52_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC2"
                     /protein_id="HEO6883108.1"
                     /translation="MKKRLVKIVTIIRNNKIRTLIFVMGSLILLFPIVSQVSYYLASH
                     QNINQFKREVAKIDTNTVERRIALANAYNETLSRNPLLIDPFTSKQKEGLREYARMLE
                     VHEQIGHVAIPSIGVDIPIYAGTSETVLQKGSGHLEGTSLPVGGLSTHSVLTAHRGLP
                     TARLFTDLNKVKKGQIFYVTNIKETLAYKVVSIKVVDPTALSEVKIVNGKDYITLLTC
                     TPYMINSHRLLVKGERIPYDSTEAEKHKEQTVQDYRLSLVLKILLVLLIGLFIVIMMR
                     RWMQHRQ"
     gene            9249..11921
                     /locus_tag="VBC52_001532"
     CDS             9249..11921
                     /locus_tag="VBC52_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEO6883109.1"
                     /translation="MKKRQKIWRGLSVTLLILSQIPFGILVQGETQDTNQALGKVIVK
                     KTGDNATPLGKATFVLKNDNDKSETSHETVEGSGEATFENIKPGDYTLREETAPIGYK
                     KTDKTWKVKVADNGATIIEGMDADKAEKRKEVLNAQYPKSAIYEDTKENYPLVNVEGS
                     KVGEQYKALNPINGKDGRREIAEGWLSKKITGVNDLDKNKYKIELTVEGKTTVETKEL
                     NQPLDVVVLLDNSNSMNNERANNSQRALKAGEAVEKLIDKITSNKDNRVALVTYASTI
                     FDGTEATVSKGVADQNGKALNDSVSRDYHKTTFTATTHNYSYLNLTNDANEVNILKSR
                     IPKEAEHINGDRTLYQFGATFTQKALMKANEILETQSSNARKKLIFHVTDGVPTMSYA
                     INFNPYISTSYQNQFNSFLNKIPDRSGILQEDFIINGDDYQIVKGDGESFKLFSDRKV
                     PVTGGTTQAAYRVPQNQLSVMSNEGYAINSGYIYLYWRDYNWVYPFDPKTKKVSATKQ
                     IKTHGEPTTLYFNGNIRPKGYDIFTVGIGVNGDPGATPLEAEKFMQSISSKTENYTNV
                     DDTNKIYDELNKYFKTIVEEKHSIVDGNVTDPMGEMIEFQLKNGQSFTHDDYVLVGND
                     GSQLKNGVALGGPNSDGGILKDVTVTYDKTSQTIKINHLNLGSGQKVVLTYDVRLKDN
                     YISNKFYNTNNRTTLSPKSEKEPNTIRDFPIPKIRDVREFPVLTISNQKKMGEVEFIK
                     VNKDKHSESLLGAKFQLQIEKDFSGYKQFVPEGSDVTTKNDGKIYFKALQDGNYKLYE
                     ISSPDGYIEVKTKPVVTFTIQNGEVTNLKADPNANKNQIGYLEGNGKHLITNTPKRPP
                     GVFPKTGGIGTIVYILVGSTFMILTICSFRRKQL"
     gene            11975..12765
                     /locus_tag="VBC52_001533"
                     /pseudo
     CDS             11975..12765
                     /locus_tag="VBC52_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
     gene            13014..13619
                     /locus_tag="VBC52_001534"
     CDS             13014..13619
                     /locus_tag="VBC52_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEO6883110.1"
                     /translation="MARDPYSDRSRYLDPKVLSSSFGAFFPADNIKVAWSNNSSTLFT
                     PPINANYTTQIQAIGTTIKSQIPESILTVTDKKSRKFSINKIDEAKEGLVGATFTLSK
                     RTTVAADHQVQGDFIPVSKETTVGRTTLTFDNLKPGFYDLKETKAPNAYVLDPKTYVV
                     VVQNSGKTTIVDEANFKEADYPMADNTSHVECVALLQRSKG"
BASE COUNT         4880 a         2342 c         2906 g         4308 t
ORIGIN      
        1 tgatatcatc aaagattatt aaaatagact ccctaaggaa gtcttctttt gaattgtaat
       61 gattatagaa agatcttcta ctaagtcgag ctttttttgc taaatcgcta actttaattt
      121 gctcaaagct cctcatcttt aacaattctg agaaagcttt taaaaatttt aaattagtgt
      181 tatctgaact taccaatata ctatcacctt tatataaaat cttaaagtta taaatcttat
      241 cgaatgtgat tatacaatac aaattcaaat aatagttcac atttatcgag ataatgtaaa
      301 acagtaaaac tttaaaaaaa gtaaaaatag ataactttat tggttaatct atttttactt
      361 ttgaaaagga gagaagttat gcttaggaag tataagcaaa gaaattataa tgtcgcacat
      421 acgaaacagc gtttttcgat taagaaattc aaatttggtg tagcatcggt attaattgga
      481 ttgacatttc taggaatgag tagtcattct gttttggcgg atgagtcaag cattatgagt
      541 actgaggtac cacaaacagc tggtgtaaaa ccggaagtta ctgttacaca gcctacttca
      601 gatgtaacaa gtcttgcgac agatacgtca acttcagcag tgttatcaac agatgcatct
      661 cattctaatc ctacagaatc agcggagcat gctgtaggat tagaatgctg aagatgcagc
      721 ggttccagtg acacacgaaa aagaagcaaa taaaacattg cgagttactt cttcttccac
      781 atctgaattc acagagtgga ctaatgtcaa ataagtagac agaaaaccgt gttattttat
      841 tgcgttaaaa taattttctt ctttctgatt aggggttagt cctagattag ccgtatgtgg
      901 gatgtaattg ttataaaaat tctcaatgta ttcaaagcag tctaattgaa cctgtttgat
      961 attttgataa tgttttcggt tgatttgtct atgctttaaa tacttgaaaa atgcttcagt
     1021 tacggcatta tcataaggat atccaggttt agaaaaagaa tgcatgatat tgttgtcatc
     1081 aattattttt ctaaattccc tggccttaaa ttgtgaccct tggtcggaat gaaagagaag
     1141 tgttccttca atctttcttt tattaagagc taattctaga gtgttacatg ctaactttgc
     1201 atccatacgg tgactcaatt tccaagcaat acattttcta gaatagagat caagtattgc
     1261 gcagagatag acatatttct tatatccaat agaaatatag gtgaagtcag tagaccatac
     1321 ttgattaggt ttgtcaggat aaaatttctg gttgagtaag ttttgaggat acgttttttg
     1381 agtcttttta agagctgttt ttggcttaac ggtagccatt ttagggagcg ccatattctt
     1441 catcagatgg taaattcttc cttcagagat ttttgtgtcg tagtctcttt gaagaatgac
     1501 tttgatagac ttcacaccaa gtcttttgtt tgctttggta taaatctcaa gtatttgttt
     1561 tttataaatt tgattatcca attctctttt tgagggctta tgttttagaa atttatagta
     1621 agtggagcga ttgccacgta aaacacgaca caaggttgtt gtggcgtgtt caaagcgtaa
     1681 ccgatagaca gcgaggagtc ttatttaagt tttgcatgaa tatggcactt gcttttttta
     1741 ggataaggtt ctcctcttca agctgcgcat tacgtttttg taactcttgt atttgcttgg
     1801 cagtaagtac agaattatct tcgagtttga attgagagta ctgtttgatc cattttgcaa
     1861 gcgcagaagt ggatacccta tagtctttac agagttcaga ctgagttttt ccagtttgat
     1921 aaaggttaac aagagattgt ttgaattctt catcatagcg tttaaatgtt gacataggtt
     1981 gtcctttcgt ttttgtgtct tttaaaacag attgtaacac acaaagtttt gtctactttt
     2041 atagtataca tctacccttt ggcaatacca aaagtttgag cagattagtt gagatgtgca
     2101 ggaagttttg attatcgcaa gagagaatca tcaagtgact catgaacacg tgtcaatctt
     2161 attgacctgc gttcatgaat tgattgtgga agtcaatcaa acacagccac tcagtcgtga
     2221 gtttcgtgaa aagtatatgg gttaggaggc tgcaattgtt tatcgttatc gtaccaatct
     2281 caaaaaagtc tttctaacag attcagaatt gaatcaatta aatgagcgga ttgctaagag
     2341 caattgtcaa aatttctcag tctatgctag aagagttctt cttaatccca atatgtcatt
     2401 tgtcacgatt aacacggatt ggaaataaca tcaatcaaat ttcgcgtgct atcaaccaaa
     2461 gccatctaat ttctcaggct cagtatcact ttcatcgaga agcacgttaa gcccaacagc
     2521 aggggggggt gctctgattc ggttaagtaa taagcaaaat cctcaccaat tttcccagta
     2581 ataagaggcg ttgttgaggt atagggatta cctgtgccat agtcgatgat ggtcacaaaa
     2641 tgaccattcc ccataaaagg gccaaccaaa acttcacctg tggccgtttt ttgatgtcaa
     2701 caccagtatt ttggatgtac cccttaacat gccctttggt atctgcaaca gagataatat
     2761 gaccaaagga agaatcaccg atgactttaa ccgtaatctt gctatcgcct ttttgattag
     2821 ccgctaatat ttgattagca atcagggtgc gaccaagagc aactgttgaa gacgacaagg
     2881 tatgatgttt ttcctgagcc aatttaacag tctcggtgct atcgaggaca taagacctga
     2941 aggcaccaga ggcagaaatt gatttaataa ttttatccat aacctatgtt atagcacaaa
     3001 gagagagttt gtatggactg gattagctga aaattttaga ctaaaaagta gtgccagtgg
     3061 attttgaaag gtaacaaaag agtcttccgt ttaaacgaga aggctgtttt tagtcgtttc
     3121 agatgaaggc aaaaggacga tgaaataatt ccgtaccttc atggattgct atgttactgg
     3181 catgaggtct cacgatattt agtaagatat tcgtttgaag atattcccac gtatttttta
     3241 aaggttttaa gaaaatgtgt ggtgtcgtaa aaatgtaata atttcgctac ttccccaatc
     3301 ggtacccctc tttgcagtag taattgtccc tccttaattt ttgcctttag aatataactt
     3361 tgcaaggaaa tgtcagagta ttttttaaaa accgagcgta agtgggaatt tgagatatat
     3421 aggtagttgg caatatcaga tactttgagt ttggagtaga ggtggttgtc cacataatgg
     3481 agaatactat tgtacatttg ctgcttgtca gagatgctct tattggttaa ggattctgaa
     3541 aaatcaataa gagctgcaca gcgaattctt gaaacatcaa taagatcagg agcctcttcg
     3601 tttaaagcca tatagtgctt taccagcgca taacttttag ccacatcagt atttttctcg
     3661 aaacttaatt ctagtaattt tgttaagtaa acaacagtta agttcttttc agctcttagg
     3721 gcagggattg aagatgaggt aacactggat gatgggaggc gattaatttc ttgctttaac
     3781 agttgagtgt tacccagctt aacgagatca ataatgtgat tgagatggtt taaaacagtg
     3841 ggtaactgaa aagagttttt cttagtatgt tttaggtgaa gaacaatatc aggatccgca
     3901 acaatctgtt ctgactcttc taataaatga ttgatggctt gttggcaact agcctcaaac
     3961 tgtgtttgga aaaaggcatc gatagacaca agaagactac gtatactggt agtaggaaaa
     4021 caagggacaa gctttatata ggataagatt tcttttttac tacgatgaga aaattgttct
     4081 agaaagcgac tggataactg ttcttgccta ttgatatcag ggctataggg ataaaatggt
     4141 ccaatagcaa taagatattg acagacagga aaaattaaga atgattcttc aaaaagaccc
     4201 tcataaacag tgatatcttg gttataaggg atagctaaat gttttaaaaa ctgatagtaa
     4261 ggcaacagat agtcttcgtt accatataac tgaacgagtt ccttgtctcg tgacatgact
     4321 gaaataggta gttgagatat ggtatgcaat gtttgaacat gtttaaaatc gaatgtaacc
     4381 atttgataga ccgccttcat tatcatttct agaatttttc tttaggtttg taaagactac
     4441 aaaataaaat gatgaaaaca actatcttgt ggatacacta aaaagacacg ctaattagca
     4501 aactctctct tcatcatctc tcaccattat tatactacta tttatatgac aaataaaggt
     4561 gattttgtta aaaatataac tttgaaaatc cacatatatt tttaatcttc cgtctgaaaa
     4621 aataaataaa aatagtaaaa ataaacacga atttaaaata agcaaatttt ttaagaaaat
     4681 ctgtgctaaa ctttaatagt tttgtgctta ataataatca gcacttacaa agaacaaagg
     4741 gaaaagcgag gagagaactt ttaatgaaat tatcgaagaa gttattgttt tcggctgctg
     4801 ttttaacaat agtggcgggg tcaactgttg aaccagtagc tcagtttgcg actggaatga
     4861 gtattgtaag agctgcagaa gtgtcacaag aacgcccagc gaaaacaaca gtaaatatct
     4921 ataaattaca agctgatagt tataaatcgg aaattacttc taatggtggt atcgagaata
     4981 aagacggcga agtaatatct aactatgcta aacttggtga caatgtaaaa ggtttgcaag
     5041 gtgtacagtt taaacgttat aaagtcaaga cggatatttc tgttgatgaa ttgaaaaaat
     5101 tgacaacagt tgaagcagca gatgcaaaag ttggaacgat tcttgaagaa ggtgtcagtc
     5161 tacctcaaaa aactaatgct caaggtttgg tcgtcgatgc tctggattca aaaagtaatg
     5221 tgagatactt gtatgtagaa gatttaaaga attcaccttc aaacattacc aaagcttatg
     5281 ctgtaccgtt tgtgttggaa ttaccagttg ctaactctac aggtacaggt ttcctttctg
     5341 aaattaatat ttaccctaaa aacgttgtaa ctgatgaacc aaaaacagat aaagatgtta
     5401 aaaaattagg tcaggacgat gcaggttata cgattggtga agaattcaaa tggttcttga
     5461 aatctacaat ccctgccaat ttaggtgact atgaaaaatt tgaaattact gataaatttg
     5521 cagatggctt gacttataaa tctgttggaa aaatcaagat tggttcgaaa acactgaata
     5581 gagatgagca ctacactatt gatgaaccaa cagttgataa ccaaaataca ttaaaaatta
     5641 cgtttaaacc agagaaattt aaagaaattg ctgagctact taaaggaatg acccttgtta
     5701 aaaatcaaga tgctcttgat aaagctactg caaatacaga tgatgcggca tttttggaaa
     5761 ttccagttgc atcaactatt aatgaaaaag cagttttagg aaaagcaatt gaaaatactt
     5821 ttgaacttca atatgaccat actcctgata aagctgacaa tccaaaacca tctaatcctc
     5881 caagaaaacc agaagttcat actggtggga aacgatttgt aaagaaagac tcaacagaaa
     5941 cacaaacact aggtggtgct gagtttgatt tgttggcttc tgatgggaca gcagtaaaat
     6001 ggacagatgc tcttattaaa gcgaatacta ataaaaacta tattgctgga gaagctgtta
     6061 ctgggcaacc aatcaaattg aaatcacata cagacggtac gtttgagatt aaaggtttgg
     6121 cttatgcagt tgatgcgaat gcagagggta cagcagtaac ttacaaatta aaagaaacaa
     6181 aagcaccaga aggttatgta atccctgata aagaaatcga gtttacagta tcacaaacat
     6241 cttataatac aaaaccaact gacatcacgg ttgatagtgc tgatgcaaca cctgatacaa
     6301 ttaaaaacaa caaacgtcct tcaatcccta atactggtgg tattggtacg gctatctttg
     6361 tcgctatcgg tgctgcggtg atggcttttg ctgttaaggg gatgaagcgt cgtacaaaag
     6421 ataactaaat aaaaggctac ttcttaagta accatgttta agaaaagaga aatagcctta
     6481 tttctctttt tgtcgttttt aaaataaagg aacatcatga aacaaacatt aaaacttatg
     6541 ttttcttttc tgttgatgtt agggactatg tttggaatta gccaaactgt tttagcgcaa
     6601 gaaactcatc agttgacgat tgttcatctt gaagcaaggg atattgatcg tccaaatcca
     6661 cagttggaga ttgcccctaa agaagggact ccaattgaag gagtactcta tcagttgtac
     6721 caattaaaat caactgaaga tggcgatttg ttggcacatt ggaattccct aactatcaca
     6781 gaattgaaaa aacaggcgca gcaggttttt gaagccacta ctaatcaaca aggaaaggct
     6841 acatttaacc aactaccaga tggaatttat tatggtctgg cggttaaagc cggtgaaaaa
     6901 aatcgtaatg tctcagcttt cttggttgac ttgtctgagg ataaagtgat ttatcctaaa
     6961 atcatctggt ccacaggtga gttggacttg cttaaagttg gtgtggatgg tgataccaaa
     7021 aaaccactag caggcgttgt ctttgaactt tatgaaaaga atggtaggac tcctattcgt
     7081 gtgaaaaatg gggtgcattc tcaagatatt gacgctgcaa aacatttaga aacagattca
     7141 tcagggcata tcagaatttc cgggctcatc catggggact atgtcttaaa agaaatcgag
     7201 acacagtcag gatatcagat cggacaggca gagactgctg tgactattga aaaatcaaaa
     7261 acagtaacag taacgattga aaataaaaaa gttccgacac ctaaagtgcc atctcgagga
     7321 ggtcttattc ccaaaacagg tgagcaacag gcaatggcac ttgtaattat tggtggtatt
     7381 ttaattgctt tagccttacg attactatca aaacatcgga aacatcaaaa taaggattag
     7441 catgggacaa aaatcaaaaa tatctctagc tacgaatatt cgtatatgga tttttcgttt
     7501 aattttctta gcgggtttcc ttgttttggc atttcccatc gttagtcagg tcatgtactt
     7561 tcaagcctct cacgccaata ttaatgcttt taaagaagct gttaccaaga ttgaccgggt
     7621 ggagattaat cggcgtttag aacttgctta tgcttataac gccagtatag caggtgccaa
     7681 aactaatggc gaatatccag cgcttaaaga cccctactct gctgaacaaa agcaggcagg
     7741 ggtcgttgag tacgcccgca tgcttgaagt caaagaacaa ataggtcatg tgattattcc
     7801 aagaattaat caggatatcc ctatttacgc tggctctgct gaagaaaatc ttcagagggg
     7861 cgttggacat ttagagggga ccagtcttcc agtcggtggt gagtcaactc atgccgttct
     7921 aactgcccat cgagggctac caacggccaa gctatttacc aatttagaca aggtaacagt
     7981 aggtgaccgt ttttacattg aacacatcgg cggaaagatt gcttatcagg tagaccaaat
     8041 caaagttatc gcccctgatc agttagagga tttgtacgtg attcaaggag aagatcacgt
     8101 caccctatta acttgcacac cttatatgat aaatagtcat cgcctcctcg ttcgaggcaa
     8161 gcgaattcct tatgtggaaa aaacagtgca gaaagattca aagaccttca ggcaacaaca
     8221 atacctaacc tatgctatgt gggtagtcgt tggacttatc ttgctgtcgc ttctcatttg
     8281 gtttaaaaag acgaaacaga aaaagcggag aaagaatgaa aaagcggcta gtcaaaatag
     8341 tcacaataat tcgaaataat aaaatcagaa ccctcatttt tgtgatggga agtctgattc
     8401 tcttatttcc gattgtgagc caggtaagtt actaccttgc ttcgcatcaa aatattaatc
     8461 aatttaagcg ggaagtcgct aagattgata ctaatacggt tgaacgacgc atcgctttag
     8521 ctaatgctta caatgagacg ttatcaagga atcccttgct tatagaccct tttaccagta
     8581 agcaaaaaga aggtttgaga gagtatgctc gtatgcttga agttcatgag caaataggtc
     8641 atgtggcaat cccaagtatt ggggttgata ttccaattta tgctggaaca tccgaaactg
     8701 tgcttcagaa aggtagtggg catttggagg gaaccagtct tccagtggga ggtttgtcaa
     8761 cccattcagt actaactgcc caccgtggct tgccaacagc taggctattt accgacttaa
     8821 ataaagttaa aaaaggccag attttctatg tgacgaacat caaggaaaca cttgcctaca
     8881 aagtcgtgtc tatcaaagtt gtggatccaa cagctttaag tgaggttaag attgtcaatg
     8941 gtaaggatta tataaccttg ctgacttgca caccttacat gatcaatagt catcgtctct
     9001 tggtaaaagg agagcgtatt ccttatgatt ctaccgaggc ggaaaagcac aaagaacaaa
     9061 ccgtacaaga ttatcgtttg tcactagtgt tgaagatact actagtatta ttaattggac
     9121 tcttcatcgt gataatgatg agaagatgga tgcaacatcg tcaataacga tgttgtgaat
     9181 ggcttactta cttatcaaat aggtgactaa tgatgattgt gaataatggt tatctagaag
     9241 ggagaaaaat gaaaaagaga caaaaaatat ggagagggtt atcagttact ttactaatcc
     9301 tgtcccaaat tccatttggt atattggtac aaggtgaaac ccaagatacc aatcaagcac
     9361 ttggaaaagt aattgttaaa aaaacgggag acaatgctac accattaggc aaagcgactt
     9421 ttgtgttaaa aaatgacaat gataagtcag aaacaagtca cgaaacggta gagggttctg
     9481 gagaagcaac ctttgaaaac ataaaacctg gagactacac attaagagaa gaaacagcac
     9541 caattggtta taaaaaaact gataaaacct ggaaagttaa agttgcagat aacggagcaa
     9601 caataatcga gggtatggat gcagataaag cagagaaacg aaaagaagtt ttgaatgccc
     9661 aatatccaaa atcagctatt tatgaggata caaaagaaaa ttacccatta gttaatgtag
     9721 agggttccaa agttggtgaa caatacaaag cattgaatcc aataaatgga aaagatggtc
     9781 gaagagagat tgctgaaggt tggttatcaa aaaaaattac aggggtcaat gatctcgata
     9841 agaataaata taaaattgaa ttaactgttg agggtaaaac cactgttgaa acgaaagaac
     9901 ttaatcaacc actagatgtc gttgtgctat tagataattc aaatagtatg aataatgaaa
     9961 gagccaataa ttctcaaaga gcattaaaag ctggggaagc agttgaaaag ctgattgata
    10021 aaattacatc aaataaagac aatagagtag ctcttgtgac atatgcctca accatttttg
    10081 atggtactga agcgaccgta tcaaagggag ttgccgatca aaatggtaaa gcgctgaatg
    10141 atagtgtatc acgggattat cataaaacta cttttacagc aactacacat aattacagtt
    10201 atttaaattt aacaaatgat gctaacgaag ttaatattct aaagtcaaga attccaaagg
    10261 aagcggagca tataaatggg gatcgcacgc tctatcaatt tggtgcgaca tttactcaaa
    10321 aagctctaat gaaagcaaat gaaattttag agacacaaag ttctaatgct agaaaaaaac
    10381 ttatttttca cgtaactgat ggtgtcccta cgatgtctta tgccataaat tttaatcctt
    10441 atatatcaac atcttaccaa aaccagttta attctttttt aaataaaata ccagatagaa
    10501 gtggtattct ccaagaggat tttataatca atggtgatga ttatcaaata gtaaaaggag
    10561 atggagagag ttttaaactg ttttcggata gaaaagttcc tgttactgga ggaacgacac
    10621 aagcagctta tcgagtaccg caaaatcaac tctctgtaat gagtaatgag ggatatgcaa
    10681 ttaatagtgg atatatttat ctctattgga gagattacaa ctgggtctat ccatttgatc
    10741 ctaagacaaa gaaagtttct gcaacgaaac aaatcaaaac tcatggtgag ccaacaacat
    10801 tatactttaa tggaaatata agacctaaag gttatgacat ttttactgtt gggattggtg
    10861 taaacggaga tcctggtgca actcctcttg aagctgagaa atttatgcaa tcaatatcaa
    10921 gtaaaacaga aaattatact aatgttgatg atacaaataa aatttatgat gagctaaata
    10981 aatactttaa aacaattgtt gaggaaaaac attctattgt tgatggaaat gtgactgatc
    11041 ctatgggaga gatgattgaa ttccaattaa aaaatggtca aagttttaca catgatgatt
    11101 acgttttggt tggaaatgat ggcagtcaat taaaaaatgg tgtggctctt ggtggaccaa
    11161 acagtgatgg gggaatttta aaagatgtta cagtgactta tgataagaca tctcaaacca
    11221 tcaaaatcaa tcatttgaac ttaggaagtg gacaaaaagt agttcttacc tatgatgtac
    11281 gtttaaaaga taactatata agtaacaaat tttacaatac aaataatcgt acaacgctaa
    11341 gtccgaagag tgaaaaagaa ccaaatacta ttcgtgattt cccaattccc aaaattcgtg
    11401 atgttcgtga gtttccggta ctaaccatca gtaatcagaa gaaaatgggt gaggttgaat
    11461 ttattaaagt taataaagac aaacattcag aatcgctttt gggagctaag tttcaacttc
    11521 agatagaaaa agatttttct gggtataagc aatttgttcc agagggaagt gatgttacaa
    11581 caaagaatga tggtaaaatt tattttaaag cacttcaaga tggtaactat aaattatatg
    11641 aaatttcaag tccagatggc tatatagagg ttaaaacgaa acctgttgtg acatttacaa
    11701 ttcaaaatgg agaagttacg aacctgaaag cagatccaaa tgctaataaa aatcaaatcg
    11761 ggtatcttga aggaaatggt aaacatctta ttaccaacac tcccaaacgc ccaccaggtg
    11821 tttttcctaa aacaggggga attggtacaa ttgtctatat attagttggt tctactttta
    11881 tgatacttac catttgttct ttccgtcgta aacaattgta aggtgtcgtt gaaattatta
    11941 aataatagaa aatgactagt tttggccttt ccctattgtc agtcagatta gttattatca
    12001 agcttctcat gccaatatta atgcctttaa aagaacagtt acaaccattg accgtacgga
    12061 gattcaacga cggttaggtc tggctaaagc ctacaatgct agtatttctg gaacaagtag
    12121 tcagtcgact caatctgtgc tgagagattc ttattctgag gagcaaaaaa ggcagggtgg
    12181 actgaatacg ctaggatgtt agaagtcaga gagcaggttg accatgtgat gattccaaaa
    12241 atcaatcagg atttaccaat ctacgctggt tcagaagagg acaatctgca acggggagtt
    12301 ggtcatctag aagggataag tttgccgatt ggaggggctt ctacacatgc ggtcttgagc
    12361 ggtcaaagag gtatgtcagc tgctcggttg tttgcggatt tggataagat gaaaaaaggt
    12421 gattattttt atgttaccaa tctgaaagaa accttggctt atcaagtgga tcgtatcatg
    12481 gtgattgaac ctagccaatt ggatgccgtg agcattgaag aggataaaga ttatgttacc
    12541 cttctgacct gtacacctta tatgggctct ttgtcaactg taatgggtga cttatcatta
    12601 acaacgagag agaatcagct tggttctctc tctttttgga tgttcaaagc gatgagaatt
    12661 ttgcttttaa aatttttaaa gttgcgaaag ccaaaggctt gtcgcttgat gtctttgata
    12721 agcttgttgg tcgcttctaa tttggcattg gaaagggata gctgagggcg tttgtgatgt
    12781 aagttttgta cttcatgaag gtcctaaaga ccattttaaa agcagaattg ccagttgtta
    12841 cgttatcatc tatgaagcta aagaattcag ttgttcgctt ttcttagaaa tgaaagagta
    12901 tgagctgata aaggtcgtta taatagcgga gctcatctga gaaaactttt atacctcaaa
    12961 gtcagtctag ctttgatatc atgaagccat taggagttat tccttatctt ttagtggcgc
    13021 gcgatccata tagtgataga tcgagatatt tagatccaaa agttctatca tcctcttttg
    13081 gcgccttttt tccagcagat aatattaagg tagcttggtc taacaactcc agcactttat
    13141 ttacaccacc tattaatgca aactacacca ctcagattca agctattggg acaacgatta
    13201 agtcacaaat tccggaatcg attttgacgg ttacggataa aaagagcagg aagttcagca
    13261 ttaacaagat tgacgaagct aaagaaggct tagtaggtgc gaccttcacc ttgtctaaac
    13321 gcacaacagt agcggcagat catcaagtac aaggagattt cattcctgtc agcaaagaga
    13381 cgacagtcgg tcggacaact cttacctttg acaaccttaa acctggattt tatgacctta
    13441 aagaaacgaa agcgccgaat gcttacgtac ttgatcctaa gacttatgtt gtggtcgttc
    13501 aaaattcagg aaaaacgaca attgtggatg aagctaactt caaagaggct gattacccaa
    13561 tggctgataa taccagccat gtggagtgcg tagcgttgct acaacgaagc aaagggtaaa
    13621 aatcctttat tttaagcact ttttcaagca ttttgtcttt attgaaaaga gtgattttaa
    13681 cataaaaaag gtattaaaaa acatattgac gtgaccgttt gttttgaagt ggcttgcgta
    13741 gacaaaaaaa tagatacgtc agataaattt ctggcattac gagaacattt ctagagtgtt
    13801 ctcttttttt agtttacgga ggaaaaatat atatggaaaa acaggattca cgagttctca
    13861 tccattggga ggggaattct ggggacaagc tcattgaaca ccaaaccagc gcaacggggt
    13921 ggtactacca agtcgatcgt agctttagtc aaccaaaagg ggaaccgccc agaatgatcc
    13981 aaaggcacta gaaagtgtcc gtaatgattc gatttcgggc ggtgatgatg tcatgggtta
    14041 tgcttatagc aaatgtactt ggggagttgc ggcacgaatt aatcagtggg actgaaactc
    14101 aaaggttgaa atggtgagaa gattaccatt accagttcaa tgggaaatgg tcaggattgg
    14161 gttggaacag ccgaaagact agatggtgaa actgatacag ttccaaaaga aggtactatt
    14221 ctctcttttt aggaaagtag ttatggttcg tatataggct acggaactat atctttcgtc
    14281 acattacatc tacagatagt accatgaatt ttgcttatat gaccaagtaa agtgaggata
    14341 tactaacaaa tgaaatattt attatcgtat ttgtccattt tatcgaaaag tttgcatatt
    14401 atcattatgt ttgataagat gcaaatataa tgatag
//
LOCUS       DATRNK010000052        13881 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.052, whole genome shotgun sequence.
ACCESSION   DATRNK010000052 DATRNK010000000
VERSION     DATRNK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13881
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.052"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..2061
                     /locus_tag="VBC52_001535"
     CDS             <1..2061
                     /locus_tag="VBC52_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO6883111.1"
                     /translation="DIFNSLMGNMGGYNSENKRYLINGREVTPEEFSQYRQTGKLPGQ
                     ELNNQNTPTNQVSADSVLTKLGTNLTDQARQHLLDPVIGRNKEIQETAEILARRTKNN
                     PVLVGDAGVGKTAVIEGLAQAIINGDVPAAIKNKEIISIDISSLEAGTQYRGSFEENI
                     QNIIKEVKETGNIILFFDEIHQILGAGSTGGDSGSKGLADILKPALSRGELTVIGATT
                     QDEYRNTILKNAALARRFNEVKVNAPSAQDTFNILMGIRNLYEQHHNVVLPDSVLKAA
                     VDLSIQYIPQRSLPDKAIDLIDMTAAHLAAQHPVTDLKSLEKEIAAQRDKQEKAVNTE
                     DFEEALKVKTRIEELQNQIDNHTEGQKVTATINDIAMSIERLTGVPVSNMGASDIERL
                     KELGNRLKGKVIGQNDAVEAVARAIRRNRAGFDDGNRPIGSFLFVGPTGVGKTELAKQ
                     LAFDMFGSKDAIVRLDMSEYNDRTAVSKLIGATAGYVGYDDNSNTLTERIRRNPYSIV
                     LLDEIEKADPQVITLLLQVLDDGRLTDGQGNTINFKNTVIIATSNAGFGNEAFTGDSD
                     KDLKIMERISPYFRPEFLNRFNGVIEFSHLSKDDLSEIVDLMLDEVNQTIGKKGIDLV
                     VDENVKSHLIELGYDEAMGVRPLRRVIEQEIRDRITDYYLDHTDVKHLKANLQDGQIV
                     ISER"
     gene            complement(2154..3098)
                     /gene="mmuM"
                     /locus_tag="VBC52_001536"
     CDS             complement(2154..3098)
                     /gene="mmuM"
                     /locus_tag="VBC52_001536"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HEO6883112.1"
                     /translation="MGRFKELLESKKTLILHGALGTELESRGCDVSGKLWSAKYLIED
                     PAAIQTIHEDYIRAGADIVTTSTYQATLQGLAQVGVSESQAEDLIRLTVQLAKAAREQ
                     VWKSLTKEEKSERIYPLISGDVGPYAAFLADGSEYTGLYDIDKQGLKNFHRHRIELLL
                     DEGVDILALETIPNAQEAEALIELLAEDFPQVEAYMSFTSQDGKTISDGSAVADLAKS
                     IDVSPQVVALGINCSSPSLVADFLQAIAEQTNKPLVTYPNSGEVYDGASQSWQSSPDH
                     SHTLLENTSDWQKLGAQVVGGCCRTRPADIADLSAHLK"
     gene            complement(3103..4479)
                     /locus_tag="VBC52_001537"
     CDS             complement(3103..4479)
                     /locus_tag="VBC52_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO6883113.1"
                     /translation="MENHRYEDEGKFQRKMTSRHLFMLSLGGVIGTGLFLSSGYTIAQ
                     AGPLGAVLSYLIGAVVVYLVMLSLGELAVAMPVTGSFHTYATKFISPGTGFTVAWLYW
                     ICWTVALGTEFLGAAMLMQRWFPDVPAWTFASFFALVIFGLNALSVRFFAEAESFFSS
                     IKVIAIIIFIILGLGAMFGLVSFEGQHKAILFTHLTANGDFPNGIVAVVSVMLAVNYA
                     FSGTELIGIAAGETDNPKEAVPRAIKTTIGRLVVFFVLTIVVLASLLPMKEAGVSTAP
                     FVDVFDKMGVPFAADIMNFVILTAILSAGNSGLYASSRMLWSLANEGMLSKSVVKINK
                     YGVPMRALLLSMAGAVLSLFSSIYAADTVYLALVSIPGFAVVVVWLAIPVAQINFRKE
                     FLKENQLEDLSYKTPFTPVLPYITIILLLISIVGIAWDSSQRAGLYFGVPFIIFCYIY
                     HKLRYKKW"
     gene            complement(4609..5259)
                     /locus_tag="VBC52_001538"
     CDS             complement(4609..5259)
                     /locus_tag="VBC52_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HEO6883114.1"
                     /translation="MSYFRNYWYRFGAILFIILAVILLVFRPDWSMLHYLLYFNFMAL
                     LAHQFEEYQFPGGASPIINYVVYDEEELMDRFPGNTQSIMLVNTIAWLLYIASIAFPQ
                     AYWLGLGVMFFSLTQLLGHGFQMNIKLKTWYNPGLATTVFLLVPIACAYIYQASAEGM
                     LTWGDWLGGFIMLIVCVLTSIIAPVQLLKDKKTNYIISPWQMDRFHKVINFVRIKK"
     gene            complement(5271..5920)
                     /locus_tag="VBC52_001539"
                     /pseudo
     CDS             complement(5271..5920)
                     /locus_tag="VBC52_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
     gene            6012..6641
                     /locus_tag="VBC52_001540"
     CDS             6012..6641
                     /locus_tag="VBC52_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO6883115.1"
                     /translation="MTESVTVNKKRIKQITQTQEDILTAFGQLLEEYPYEKIQISQIA
                     KKSGYARRTLYRHFDSRDDLLTLFIERLTLNLFKQLGQLEQATFSQVFQNFFSYWSDY
                     KSLLLILRKNDLLPQFQQSWFRHIDLIELGRGDLSSNTYAQRFAIGGIFSVLIEWIHQ
                     DCQTSIEELTQVSFDIINHLKNKKELCSSLNSYCKALFVINSQIITQIK"
     gene            complement(6622..7218)
                     /locus_tag="VBC52_001541"
     CDS             complement(6622..7218)
                     /locus_tag="VBC52_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEO6883116.1"
                     /translation="MAEEFLNTHNASILLSAANKSHYPQDDLPEVALAGRSNVGKSSF
                     INTLLGRKNLARTSSKPGKTQLLNFYNIDDKLRFVDVPGYGYAKVSKTERAKWGKMIE
                     EYLVTRDNLRVVVSLVDFRHDPSADDIQMYEFLKYYEIPVIIVATKADKIPRGKWNKH
                     ESSIKKKLNFDKKDHFIVFSSVDRTGLDESWDTILSEL"
     gene            complement(7229..8455)
                     /gene="clpX"
                     /locus_tag="VBC52_001542"
     CDS             complement(7229..8455)
                     /gene="clpX"
                     /locus_tag="VBC52_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEO6883117.1"
                     /translation="MAGNRNNDMNVYCSFCGKSQDEVKKIIAGNGVFICNECVALSQE
                     IIKEELAEEVLADLAEVPKPKELLEILNQYVVGQDRAKRALAVAVYNHYKRVSYTESS
                     DDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENIL
                     LKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTVA
                     SVPPQGGRKHPNQEMIQINTKNILFIVGGAFDGIEDLVKQRLGEKIIGFGQTSRKIDD
                     NASYMQEIISEDIQKFGLIPEFIGRLPVVAALELLTAEDLVRILTEPRNALVKQYQTL
                     LSYDGVELEFDQDALLAIADKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVRI
                     TKAAVEGTDKPVLETA"
     gene            complement(8673..9167)
                     /locus_tag="VBC52_001543"
     CDS             complement(8673..9167)
                     /locus_tag="VBC52_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO6883118.1"
                     /translation="MTKQIIAIWAEDEDHLIGVNGGLPWRLPKELHHFKETTMGQALL
                     MGRKTFDGMNRRVLPGRETIILTKDEQFQADGVTVLNSVEQVIKWFQEHNKTLFIVGG
                     ASIYKAFLPYCEAIIKTKVHGKFKGDTYFPDVNLSEFKVISRDYFEKDEQNAHAFTVT
                     YFEK"
     gene            complement(9247..10086)
                     /locus_tag="VBC52_001544"
     CDS             complement(9247..10086)
                     /locus_tag="VBC52_001544"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEO6883119.1"
                     /translation="MTKADLLFKDNITKIMSEGVFSEQARPRYKNGEMANSKYITGAF
                     AEYDLSKGEFPITTLRPIPIKSAIKEIFWIYQDQTNDLVVLNDKYGVTYWNDWEVGHT
                     GTIGQRYGAVVKKHNIISKLLKQLEDNPWNRRNVISLWDYEAFEETEGLLPCAFQTMF
                     DVRRVNGELYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWRVGKFFYFINNL
                     HIYDNQFEQAQELLKRQPSECNPKLVLNVPDGTDFFDIKPDDFALVDYDPIKPQLRFD
                     LAI"
     gene            10135..11412
                     /locus_tag="VBC52_001545"
     CDS             10135..11412
                     /locus_tag="VBC52_001545"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEO6883120.1"
                     /translation="MRNKTLKVKAFFHIFSFFMVKWNLAKMTFIQKGLPMKIGIDKIG
                     FATSQYVLEMTDLAIARQVDPEKFSKGLLLDSLSITPVTEDIVTLAASAANDILSDED
                     KETIDMVIVATESSIDQSKAASVYVHQLLEIQPFARSFEMKEACYSATAALDYAKLHV
                     EKHPDSKVLVIASDIAKYGIKSTGESTQGAGSIAMLISQNPSILELKEDRLAQTRDIM
                     DFWRPNYSDVPYVNGMFSTKQYLDMLKTTWKEYQKRFNTSLSDYAAFCFHIPFPKLAL
                     KGFNKILDNNLDEQKKAELQENFEHSITYSKKIGNCYTGSLYLGLLSLLENSQNLKAG
                     DQIAFFSYGSGAVAEIFTGQLVDGYQNKLQSDRMDQLNKRQKITVTEYEKLFFEKTIL
                     DENGNANFNTYRTGTFSLDSICEHQRIYKKINN"
     gene            11414..12697
                     /locus_tag="VBC52_001546"
     CDS             11414..12697
                     /locus_tag="VBC52_001546"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEO6883121.1"
                     /translation="MTKISWTGFSKKSPEERIHYLEEQDFLADSSLEIVTNQDLLSLS
                     LANQMAENVIGRIALPFSLVPDVLVNGKVYQVPYVTEEPSVVAAASFAAKIIKRSGGF
                     LTTVHNRKMIGQVALYDVQDSQHAKESILNQKQQLLEIANAAHPSIVKRGGGACDLTI
                     EIKEGFLIVYLMVDTKEAMGANMVNTMMEALSSPLEDISKGKSLMSILSNYATESLVT
                     ATCRVDLRFLSRQKEEAIKLAQKMTMASQLAQVDPYRASTHNKGIFNGIDAIVLATGN
                     DWRAIEAGAHTYAVKDGQYRGLSKWSYKVDDNFLEGTLTLPMPVATKGGSIGINPSVQ
                     LAHDLLGRPNAKELASIVLSIGLAQNFAALKALVSTGIQAGHMKLQAKSLALLAGAKE
                     EQISEVVKQLLDSKHMNLETAQKIVNKLTKSKKRI"
     gene            12789..13238
                     /locus_tag="VBC52_001547"
     CDS             12789..13238
                     /locus_tag="VBC52_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEO6883122.1"
                     /translation="MNKNQYPKWEELPELDLYLDQVLLYVNQLINPKTITNDKLLTAS
                     MINNYVKHNYISKPIKKKYNRRQVARLIVITAFKQVFAIQEISQTLEILTADNHSEEA
                     YNGFAACMNKEEVHDLPPVVISACQTLNLYQETQKLVLELGGKNKNA"
     gene            13231..>13881
                     /locus_tag="VBC52_001548"
     CDS             13231..>13881
                     /locus_tag="VBC52_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEO6883123.1"
                     /translation="MLKEMSIKLSPQLSFGEELANSITHAVGALLMLILLPITAVYSH
                     NHFGLQAALGTSIFVTSLFLMFLSSSIYHSMTYSSLQKYVLRMIDHSMIYIAIAGSYT
                     PVALSLIGGWLGYLIIFLQWGITLFGILYKIFAPKINDKFSLVLYLIMGWLVIFIFPA
                     IITKTGPAFWGLLLAGGICYTIGALFYARKRPYDHMIWHLFILLASILQYIGIVYFM"
BASE COUNT         4618 a         2751 c         2446 g         4066 t
ORIGIN      
        1 gatattttta attctctaat gggaaatatg gggggctata atagtgagaa taaacgatac
       61 ttaatcaatg gacgggaagt tactcctgaa gaattcagtc aatatcgtca aactggaaaa
      121 ctccccggtc aagagctaaa taaccaaaat actcctacaa accaagtctc tgcagatagt
      181 gttttgacta aattaggaac taatttaacc gatcaagcac gtcagcattt gctagatcca
      241 gttattggac gaaacaaaga aattcaggaa actgcagaaa ttctagctcg tcgtactaaa
      301 aataatcctg ttctagttgg tgatgcaggt gttggtaaaa cagctgttat tgaaggtttg
      361 gcacaagcaa ttataaatgg tgatgtccct gctgctatca aaaataaaga aatcatttcg
      421 atagatatct ctagccttga agctggcacc caatataggg gtagttttga agaaaatatt
      481 caaaatatca taaaagaagt taaggaaaca ggaaatatta ttctcttctt tgatgaaata
      541 caccaaatat taggagccgg ttcaactggt ggtgatagtg gctctaaggg gctcgcagat
      601 atacttaagc ctgcactttc acgtggtgaa ttaacggtta taggtgcaac tactcaagat
      661 gaatatcgta acaccattct caagaatgct gccttagcac gccgctttaa cgaagttaaa
      721 gtcaatgcac cttctgcaca agatactttc aatatcctta tggggataag aaacctctat
      781 gagcaacatc ataatgttgt tttacctgac tctgttttga aagcagctgt agatttatct
      841 attcaataca taccacaaag aagtctccct gataaagcaa ttgaccttat tgatatgact
      901 gctgcacacc tagctgcgca acatcccgta actgacctta aatccttaga aaaagaaatc
      961 gctgcgcaaa gagacaagca agaaaaagct gtcaatactg aagattttga agaagctctt
     1021 aaggtcaaga cacgcattga agagttacaa aaccagattg ataatcatac cgaaggacaa
     1081 aaagtcactg caactatcaa tgatatcgct atgtcaattg agcgtctaac tggagttcct
     1141 gtatcaaata tgggtgctag tgatattgaa cgcttaaaag aacttggcaa tcgtctaaaa
     1201 ggtaaagtca ttggtcaaaa tgatgctgtt gaggcagttg caagagctat tcgccgaaat
     1261 cgtgctggtt tcgatgatgg taatcgtccg attggtagct ttctatttgt tggaccaact
     1321 ggagttggta aaactgagct agcaaagcaa ttagcttttg atatgttcgg atcaaaggat
     1381 gctatcgtta gacttgatat gtctgaatat aatgatcgta cagcagtttc taagctaatt
     1441 ggagcgactg ctggttacgt tggttatgac gacaatagta atacactaac agaacgtatc
     1501 cgtcgaaatc cttattctat tgttcttctt gatgaaatcg aaaaagctga cccgcaagtt
     1561 atcacgcttc tccttcaagt tctagatgat ggccgtttaa ctgatggtca aggaaacact
     1621 attaacttta aaaatactgt tatcattgca acctcaaatg caggttttgg aaacgaagca
     1681 tttacaggtg acagcgataa agacttgaaa attatggaac gaatttctcc atatttccgt
     1741 ccagaatttc taaatcgttt caatggtgtt attgaattct ctcacctaag caaagatgac
     1801 ttaagcgaaa ttgtagattt gatgcttgat gaagttaacc aaacaattgg caaaaaagga
     1861 attgaccttg tggtagatga aaatgttaaa tcacacttaa ttgaactggg ttatgacgaa
     1921 gcaatgggag tacgtccatt gcgccgtgtc atcgagcaag aaattcgaga tcgcatcaca
     1981 gactactatc tcgatcatac agacgttaaa cacctaaaag ctaatttgca agatggccaa
     2041 atcgtcattt ctgaaagata aaaacaaaac ccgtttggaa gaatccaagc gggttttcta
     2101 gtggattgaa aaaggaaatc aaacttaccc taaccttctt caagatactt tacttatttt
     2161 aaatgtgcag agaggtcagc aatatcagct ggtctagttc ggcaacaacc tccgacaact
     2221 tgggcgccaa gtttttgcca gtcacttgta ttttcaagta gggtgtgtga atgatcaggg
     2281 ctagattgcc aagattggct tgccccatca taaacctctc cagagtttgg ataggtcaca
     2341 agaggcttgt tagtctgctc tgctattgct tgcaagaaat cagcaactaa cgatggactt
     2401 gaacagttaa tgcccagcgc cacgacctgt gggctaacat caatagattt cgccaagtct
     2461 gcaacagcac taccatctga gatggtctta ccatcttgag aggtgaatga catataagcc
     2521 tcaacctgag ggaaatcctc ggcaagtagc tcaataagag cctcagcctc ctgagcgttt
     2581 ggaatggttt caagtgccag aatgtcaaca ccctcatcta aaagaagctc aatacgatgg
     2641 cgatggaagt ttttaagacc ttgcttatca atatcgtaga gtccagtgta ctcagagcca
     2701 tccgctaaaa aggcagcgta gggaccaaca tccccagaaa tcaaaggata gattctctct
     2761 gatttttctt cttttgtcaa acttttccaa acttgctcac gcgcagcttt tgccaactga
     2821 acagtcaagc gaatcaaatc ttctgcctga ctttcagaga caccaacttg agctagtcct
     2881 tgtaaagtcg cctgataagt tgatgtcgtc acaatatcag cgccagcacg aatataatct
     2941 tcatgaatgg tctgaatagc tgctggatcc tcaatcagat atttggctga ccaaagtttc
     3001 ccagaaacat cacaacctct actctcaagc tcggtcccca aggcaccatg taggatgaga
     3061 gtttttttac tctctaaaag ttctttaaat cttcccataa gatcaccatt tcttgtaacg
     3121 tagtttgtga taaatatagc agaaaataat aaatggaaca ccgaagtaaa gcccagcacg
     3181 ttgtgagcta tcccaagcaa tccctacaat cgaaatcaag agcaaaatga ttgtgatata
     3241 agggagaact ggtgtaaaag gtgttttgta agacaaatct tctagttgat tctctttcaa
     3301 gaattccttg cggaaattga tttgtgcgac tggaatggct agccatacga caacaacagc
     3361 aaagcccggg attgaaacca aggctagata aactgtgtct gcagcgtaaa tacttgaaaa
     3421 gagcgacagc actgctcctg ccattgacaa gagaagagca cgcattggga caccgtattt
     3481 attgattttc acgacagatt ttgacaacat accttcattg gcaagggacc agagcatacg
     3541 gcttgatgcg tagagacctg agttaccagc agacaggatg gctgtcaaga taacaaagtt
     3601 catgatatcc gccgcaaaag ggactcccat cttgtcaaag acatcaacga atggtgctgt
     3661 ggatacgcct gcctctttca ttggcaatag cgaagctagg acaacaattg tcagtacaaa
     3721 gaaaacaacc aagcgaccga ttgtcgtttt aatagccctt ggcacagctt cttttggatt
     3781 atccgtttca cctgccgcaa taccaatcaa ctcagtacca gagaaggcat agttaacagc
     3841 caacatgact gagacaactg caacgatacc atttggaaag tcaccattgg cagtcagatg
     3901 agtgaaaaga atagctttgt gctgaccttc aaaggaaact agaccaaaca tagcacctaa
     3961 gcccaagata ataaagataa tgatagcaat aaccttaata cttgagaaga aagactctgc
     4021 ttctgcaaaa aagcgcacac tgagagcatt taagccaaaa atcacaaggg caaagaaaga
     4081 agcaaatgtc caagccggca catctgggaa ccagcgctgc atgagcatgg cagcacctaa
     4141 aaattcagtt cccaaggcga ccgtccaaca aatccaatat agccaggcaa cagtaaaacc
     4201 tgtgccagga ctgataaact tagtggcata agtgtggaat gaccccgtca ccggcatggc
     4261 aactgccaat tcaccaagtg atagcatgac caaataaacc acaacggcac caatcaaata
     4321 agacagcaca gctccaagcg gaccagcctg tgcaatggta taacctgaac tcaagaaaag
     4381 cccagtcccg ataacaccac ctagagataa cataaagaga tgacgactgg tcatcttacg
     4441 ctggaattta ccttcatctt cataacgatg attttccata acaacctcca agaaactaat
     4501 acttttatcc taccacaatc tagtcgtatt ggggaatagg tattagctat catatatgat
     4561 aaagaaaaac tattacaaaa aatcagcgaa tcataaatag cggtaacctt atttttttat
     4621 ccttacaaaa ttaatgacct tatgaaaacg gtccatttgc caaggactaa taatataatt
     4681 ggtcttctta tccttcaata gctgtacagg tgcaataata ctagttagta cacagacaat
     4741 caacatgata aaaccaccta gccaatctcc ccaagtgagc attccttctg cactagcttg
     4801 atagatgtat gcgcaagcta ttggtactag gagaaatact gtcgttgcta gaccaggatt
     4861 ataccaagtt ttaagtttaa tattcatctg aaaaccatga cccaagagct gcgttagact
     4921 aaagaacatg actcctaatc caagccaata agcttgagga aaagcaatac tagcaatgta
     4981 aagcaaccaa gcaatagtat taaccaacat aatagactga gtattgcctg gaaaacgatc
     5041 catcagctct tcttcatcat aaacaacata gttaatgata ggtgatgccc caccgggaaa
     5101 ctgatattct tcaaattgat gcgctagaag tgccataaag ttaaaataca atagatagtg
     5161 aagcattgac cagtcaggtc taaaaacaag caatattact gctaaaataa taaataaaat
     5221 tgctccaaaa cgataccagt aatttctaaa atatgacatg ataatacctc ctatttaaac
     5281 tgtgtttcac gaaaatgtgt taaaaatggt tttgagcgtg aaagatcaac atcactaaat
     5341 gcgtaatttg aatgtcgacc taaacgctta tagattgggg aacgaaaggc tataaagtag
     5401 ttaagaacaa tccatatcag cctaaaaacc aatcaagtcc agaatacaac ttccaatcat
     5461 gagctaaata atagacagaa acagggacaa gacctactaa ggtcgtaaga agtcctggat
     5521 tataccactt cttcaaggaa agattaaaaa agaaaagatg catggcaagt tctgcaaatg
     5581 caaaaatcat gactgcaagt gttaaaaatg gtatattggg acaaaaaatc ggcaaaagat
     5641 aaacaatcac tgcaaaccac tgattcccga aaaaggctaa tagatggttt aagtcccact
     5701 tactagtatc agtttcaacc gatttaagtt caactagcat agcgataagc ggaaaacctc
     5761 cagggaaacc aaattcttcg aaaaagtgta attaaataaa aagtcccgat ataagtagtg
     5821 actgcactcg taaatcccaa tttccaaaaa ataatgctaa ggcaaaaaga gaggcaagat
     5881 agggacaaaa gaggtaccat tttgttatca atgttttcat aagatgacct ccaagatttg
     5941 tgacatttga taccatatag tataatcaag tgaacctata atatcaatac ctttggaggt
     6001 tttatgcaca aatgactgaa agtgtgacag ttaataaaaa gagaataaaa caaatcactc
     6061 aaacacaaga ggatatctta acagcttttg gccaactttt ggaagagtat ccctatgaga
     6121 aaattcagat cagccaaatt gccaagaaat ctggctatgc tagaaggacc ctttaccgac
     6181 actttgacag tcgagatgac ctacttaccc ttttcatcga aagactaaca ttaaatctct
     6241 ttaagcaact tggtcaattg gaacaagcta cttttagcca agttttccag aattttttta
     6301 gttattggtc tgattataaa tcccttcttc tcattcttag aaagaatgac ctactccctc
     6361 aatttcaaca atcctggttc cgacatattg atttgataga gttaggtcgt ggtgatttat
     6421 cttccaatac ctatgctcag cgatttgcga ttggtggtat tttcagtgtt ctaattgagt
     6481 ggattcatca agactgccaa acaagtatcg aagaattaac tcaagtttcc ttcgatatta
     6541 tcaatcattt aaaaaataaa aaagagcttt gcagtagtct caacagctac tgcaaagctc
     6601 tttttgtcat aaactctcaa attataactc agataaaata gtatcccatg attcatcaag
     6661 acctgtgcgg tcaacagatg aaaagacgat gaaatggtcc tttttatcaa agtttaattt
     6721 cttcttaata gacgactcat gtttattcca cttgccacga ggtattttat ctgcctttgt
     6781 cgctacaata attactggaa tttcatagta cttaagaaat tcatacattt gaatatcgtc
     6841 cgcacttgga tcatgacgaa aatcaactaa actaacaaca acacgaaggt tatcacgtgt
     6901 tacaaggtac tcttcaatca tcttgcccca cttagcacgc tcagttttag acaccttagc
     6961 gtagccatat ccagggacat cgacaaaacg cagtttgtca tcaatgttat aaaagttgag
     7021 tagttgtgtc ttacctggct tactcgacgt acgcgctagg tttttacgac caagaagcgt
     7081 gttgataaag cttgatttac caacatttga acgaccagcg agagcaactt ctgggagatc
     7141 atcctgagga taatgtgact tgttagctgc actcaataaa atagacgcat tatgagtatt
     7201 taaaaattct tctgccatct ctatctccct aagccgtctc taaaacaggc ttgtcagtac
     7261 cctcaacagc agcctttgtg atacgaactt ttgttacatc ttcttggctt ggaatttcaa
     7321 acatgatatc aagcatcgtt tcttcaataa tagaacgtaa accacgtgca ccagtcttgc
     7381 gctcgatagc cttatcagcg atagccaata gagcatcctg atcaaattcc aattctacac
     7441 catcataaga taataaggtt tggtattgtt taaccaaagc attgcgtggt tctgtcagaa
     7501 tacgaaccag atcttctgca gtaagaagtt ctaacgctgc aactactggt aaacggccaa
     7561 taaactctgg aatcagtcca aacttttgaa tatcctcaga aattatctct tgcatataag
     7621 aagcgttgtc atcaattttg cggcttgtct gtccaaaacc aataattttt tcgcctaaac
     7681 gttgcttcac aaggtcttca ataccatcaa aagcaccacc gacaataaaa aggatgttct
     7741 tggtattaat ttgaatcatt tcttggttag gatgtttacg cccaccctgt gggggaacac
     7801 ttgctaccgt accctcaata attttaagaa gggcttgctg tacaccttca ccagatacat
     7861 cacgtgtgat agaaacattt tcgcctttct tagcaatttt atctatttca tcaacgtaga
     7921 taataccacg ctcagcacgt tcgacattat aatcagcagc ttgaatcaat ttaagaagaa
     7981 tattctcaac atcttctcca acgtatcctg cttcggtcaa tgaagtcgca tctgcaatag
     8041 caaacggtac attaaggctt ttagctagtg tttgtgctaa gaaggttttt cctgagccag
     8101 ttggaccaat catcaaaatg ttggattttt gcaaatctac atcatcgtca ctactctcgg
     8161 tataactaac acgcttgtaa tgattgtaga cagcaactgc taaagcacgt ttagcacgat
     8221 cttgccctac aacatattga tttaatattt ctaatagttc cttaggtttt ggtacttctg
     8281 ctaaatcagc aagtacttcc tcagctaatt cttccttaat aatttcttgt gataaggcca
     8341 cacattcatt acaaatgaaa acaccattac ctgcaataat tttttttact tcatcttggc
     8401 ttttgccaca aaatgaacaa tagacattca tatcgttatt acgattccca gccatttttt
     8461 cctcaacttt tctattcatt taatttaaaa taaggttcca taaaaggcat ggattgtgtt
     8521 gacaaggtta gtgaaagcat tcatcaaaaa aaccgttcct aatccatatc tttttttctt
     8581 tatagcagga attgcacata aaacacaaat cacagctaaa aatgcggtaa agaccccaaa
     8641 aaggctacgt tgcattgaat agtattcctt ccttattttt caaaatatgt cacagtaaaa
     8701 gcatgagcat tctgctcatc cttttcaaaa taatctctag agataacttt aaactcagat
     8761 agattaacat caggaaaata ggtatcacct ttaaattttc catgaacttt agtttttatg
     8821 attgcttcac aataaggcag aaatgcttta taaatacttg caccacctac aataaataag
     8881 gtcttattat gttcctgaaa ccattttata acttgttcaa cactatttag gactgtcact
     8941 ccatctgctt ggaattgttc atcttttgtt aagatgattg tctctctacc aggtaaaaca
     9001 cgacggttca ttccatcaaa ggtctttcgt cccataagca aagcctgccc catggtcgtt
     9061 tctttgaagt gatgtaactc tttaggaagc ctccatggta aaccgccatt aactccaatc
     9121 aaatggtctt catcttcagc ccaaatggca attatttgct ttgtcatgac ttttttcctc
     9181 actaacttat ctctagatat tctaacaaat tttacctcaa attgcttgtt aaaaaacatg
     9241 agatagttag attgctaaat caaaacgtaa ttgaggttta attgggtcat aatcaactaa
     9301 cgcaaaatca tctggtttaa tatcaaaaaa gtctgttcca tctggaacat tcaatactag
     9361 tttggggtta cactcagacg gttggcgttt taagagttcc tgagcttgtt caaactggtt
     9421 atcataaata tgaagattat tgataaaata gaaaaacttc ccaactctcc aaccaaaatg
     9481 ctttgcaatc atcatctgaa gtgctacata ctgcatagca ttaatatgat gtgcaactaa
     9541 catgtcatta gaacgttgtg ttaatgtggc atccaaataa agctcaccat taacgcgtct
     9601 cacatcaaac atcgtttgaa aagcacaagg tagtaggcct tctgtttctt caaatgcttc
     9661 atagtcccat aatgaaataa cattacgacg attccaaggg ttatcttcaa gctgttttaa
     9721 gagttttgat ataatattat gttttttaac aactgcaccg tagcgctgac ctatcgtgcc
     9781 agtatggcca acttcccaat cattccagta tgtcacacca tatttatcat tcaaaactac
     9841 taaatcattt gtctgatctt gataaatcca gaaaatttct ttaatagcag attttattgg
     9901 aattggacgt agagttgtaa taggaaattc accctttgat aaatcatact ctgcaaaggc
     9961 acctgtaata tacttagaat tagccatttc cccattttta tatctaggtc tggcttgctc
    10021 gctaaataca ccttcagaca taattttcgt gatattgtct ttaaataata aatcggcttt
    10081 tgtcatgaga tacctccaaa gtcttatttt atcagatttt taaaagatat tctagtgaga
    10141 aacaagactt tgaaggttaa agcttttttt catattttta gcttctttat ggtaaaatgg
    10201 aatttagcaa aaatgacttt tattcagaaa ggtttaccaa tgaaaatagg aattgataag
    10261 attggttttg caaccagtca atatgtcctt gaaatgactg atttggctat tgctcgccaa
    10321 gttgaccctg aaaaattcag caagggatta ttgttagatt cacttagtat tacgccagtt
    10381 acagaagata tcgttacact tgcagcctct gcagctaatg atattttatc tgatgaggac
    10441 aaagaaacta ttgatatggt gattgtcgct acagaatcaa gtatcgatca gagtaaggcg
    10501 gcttctgtct atgtacatca acttttagag attcaacctt tcgcaaggtc attcgagatg
    10561 aaagaagcat gctatagcgc aactgctgct cttgattacg ctaaattaca tgtagaaaaa
    10621 catccagatt ctaaagtatt agtgatagct tcagatattg ctaaatatgg cattaaatct
    10681 actggagaat ccactcaagg tgcaggcagt atcgctatgc ttattagtca aaacccatcc
    10741 attctagagc taaaagagga ccgtctagcg caaacccgtg atattatgga cttttggcga
    10801 ccaaattatt ctgatgttcc ttatgttaat ggcatgtttt caacaaaaca atatctagat
    10861 atgctaaaaa ctacttggaa agaatatcaa aaacgtttta atactagtct aagtgactat
    10921 gctgcttttt gtttccatat cccttttcca aaattagctt taaagggctt taataaaatt
    10981 ctagataata acctagatga acaaaagaaa gctgaactac aagaaaattt tgagcactct
    11041 attacttata gtaagaaaat tggaaattgc tatactggct cattgtatct aggacttcta
    11101 tcgttactag aaaatagtca aaatttaaaa gcaggtgatc aaattgcctt cttttcatac
    11161 ggtagcggag ctgttgctga aatttttacc ggtcagttag tagacggcta ccaaaataaa
    11221 cttcagagcg atcgtatgga tcaactcaat aaacgtcaaa aaattacagt aactgagtat
    11281 gagaaactct tctttgaaaa aacaatccta gacgaaaatg gaaatgccaa cttcaacacc
    11341 taccgtactg gcactttttc tctagactct atttgcgaac atcaacgtat ctacaagaaa
    11401 attaataatt aatatgacaa aaatttcctg gacaggattt tcaaaaaaat cccctgaaga
    11461 acgcattcat taccttgaag aacaagattt tttagctgat tcttccctag aaatagtgac
    11521 gaatcaggac ttactctctt tatcactagc caatcaaatg gctgaaaatg tcatcggtag
    11581 aatagcctta ccgttttcac ttgtcccaga tgttttggtt aacggtaaag tctatcaagt
    11641 cccatatgtc actgaagaac cgtcagttgt tgctgctgca agttttgctg ctaaaattat
    11701 aaaacgatct ggtggttttc tgacaaccgt ccataatcgt aaaatgattg gacaagtagc
    11761 cctttatgat gtacaagata gtcaacacgc taaagaatct atattaaatc aaaaacagca
    11821 attattagaa atagccaacg ctgctcatcc atcaatagtc aagcgtggag gcggtgcttg
    11881 cgatctaact attgagatta aagaaggctt tttgattgtt tatcttatgg tcgatacaaa
    11941 agaagccatg ggtgctaata tggttaacac catgatggaa gcattatctt cgccattaga
    12001 agacatttca aaaggcaaaa gcttaatgtc aattttatct aactatgcta ctgaatcttt
    12061 agtgactgct acatgccgag ttgatttaag atttctaagt cgccaaaaag aggaggccat
    12121 caagttagct caaaaaatga cgatggcaag tcaattagct caagtagacc cttatcgtgc
    12181 tagcacacat aataaaggta tttttaatgg cattgatgct atcgtcttag ctactggaaa
    12241 tgattggcga gctattgaag ctggagcgca tacctatgct gtcaaagatg gacaatatcg
    12301 aggattatct aaatggtctt ataaggttga tgataatttt ctagaaggta ctctgacgct
    12361 accgatgcct gttgcaacaa aaggtggatc catcggtatt aaccctagtg tccaactagc
    12421 tcacgatcta ctaggtcgtc ctaatgctaa agagttggca agtattgttc tttcgattgg
    12481 actagctcaa aattttgcgg ctcttaaggc attggttagc acaggaattc aagctggtca
    12541 catgaaactt caagcaaagt cgttggcact acttgctgga gctaaagaag aacaaatttc
    12601 agaagtcgtt aaacagcttc tagactctaa acacatgaat ctagagacag cacagaaaat
    12661 tgtcaataaa cttactaaaa gtaaaaaaag aatttgagaa aaatcatctc aaattctttt
    12721 tttacttgac ttgaatttcc ataagtgtat aatctagtta taaaaactag attatatgga
    12781 gttttaaaat gaataaaaat caatacccaa aatgggaaga attacctgaa ctagatctgt
    12841 atcttgacca agtacttctc tatgtgaatc aattaattaa tcctaaaaca ataactaacg
    12901 ataaactact caccgcttct atgatcaaca attatgtcaa acataattat atatccaaac
    12961 ctattaaaaa aaaatacaat cgccgtcaag tagcacgatt gattgttatt acagctttta
    13021 aacaagtttt tgctattcaa gaaatcagtc aaacattaga aattctgaca gctgacaatc
    13081 attctgagga agcatataac ggatttgctg cttgtatgaa caaagaagag gttcacgatt
    13141 taccaccagt tgtcattagt gcctgtcaga cgttgaacct ttatcaagaa acacaaaaat
    13201 tagttttaga actaggagga aaaaataaaa atgcttaaag aaatgtctat caaattaagt
    13261 cctcaacttt cctttggcga agagcttgca aatagtatca ctcacgctgt aggagctttg
    13321 cttatgctaa tcctattacc aatcaccgct gtctatagtc acaaccactt cggattacaa
    13381 gcagcacttg gtacctcaat atttgtcact tcattatttt tgatgttttt atcatcaagt
    13441 atttaccact ccatgactta tagcagtctc caaaaatatg ttctacgcat gattgatcac
    13501 tctatgattt atattgctat tgcaggtagt tatactcctg tcgcattgtc acttattggc
    13561 ggctggttag gatatctcat cattttttta caatggggga ttacattatt tggtatcctt
    13621 tataaaatct tcgctcccaa aatcaatgat aaatttagcc ttgtacttta cctcattatg
    13681 ggatggctgg ttatctttat tttccctgct attatcacaa aaactggacc agctttttgg
    13741 ggattactct tggctggtgg tatttgctat actatcggtg cactctttta tgctcgcaaa
    13801 cgtccttatg atcacatgat ttggcacctt ttcattctat tagcttctat tttacaatac
    13861 attggaattg tctacttcat g
//
LOCUS       DATRNK010000053        13878 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.053, whole genome shotgun sequence.
ACCESSION   DATRNK010000053 DATRNK010000000
VERSION     DATRNK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13878
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.053"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(391..1986)
                     /locus_tag="VBC52_001549"
     CDS             complement(391..1986)
                     /locus_tag="VBC52_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEO6883124.1"
                     /translation="MDLQGGKLISNKVKIVRLLNKSKKSLLRGIFSRTTVIAILLILQ
                     LLFLLASYSWLEQYRVWLATVEHILTIGAVLYLVNSEMDALSRVTWLILVMIAPLLGA
                     MFLMYTKFDWGYRGLKQRLETLIDESQIYLEDDPETLNQLKSSTSTTYHLVQYFEKAH
                     GNFPVYRNTDVTFLPTGEAFFEKMKEELLKAKKYIFLEFFIIDEGIMWGEILSILEQK
                     VEEGVEVRILYDGMIEITKLSFDYTKRLEKIGIKAKAFSPISPFISTYYNYRDHRKIV
                     VIDGVVGMTGGVNLADEYINHIELFGHWKDSGIMLKGKAVDSFLLLFLQMWSITEEKM
                     LVAPYLGVHDDLVENEGYVIPYGDSPLDTDKVGENVYIDILNHAREYVYIMTPYLILD
                     SELEHAIQFAAERSVDVRIIMPGIPDKPIPYALAKTYYQALTKSGVKIYEYTPGFVHS
                     KIFLSDNTKAVVGTINLDYRSLYHHFECAVYLYKVDAIQDIYRDYMDTLNKSRLVSLK
                     DINNIPKFQKVIGIVTKTIAPLL"
     gene            complement(2104..3774)
                     /locus_tag="VBC52_001550"
     CDS             complement(2104..3774)
                     /locus_tag="VBC52_001550"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEO6883125.1"
                     /translation="MKTDIEIAQSVALKPIAEIVEQVGIGFDDIELYGKYKAKLSFDK
                     IEAVRSQKVGKLILVTAINPTPAGEGKSTMSIGLADALNKIGKKTMIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELDIDQRR
                     VIWKRVVDLNDRALRQVIVGLGSPVNGIPREDGFDITVASEIMAILCLATDLSDLKKR
                     LSNIVVAYSRDRKPIYVKDLKIEGALTLILKDAIKPNLVQTIYGTPALVHGGPFANIA
                     HGCNSVLATSTALRLADYVVTEAGFGADLGAEKFLDIKTPNLPTSPDAIVIVATLRAL
                     KMHGGVSKEDLSQENVKAVKRGFTNLERHVNNMRQYGVPVVVAINQFTADTESEIATL
                     KTLCSNIDVAVELASVWEDGADGGLELAQTVANVIETQSSNYKRLYNDEDTIEEKIKK
                     IVTKIYGGNKVHFGPKAQIQLKEFSDNGWDKMPICMAKTQYSFSDNPNLLGAPTDFDI
                     TVREFVPKTGAGFIVALTGDVLTMPGLPKKPAALNMDVLEDGTAIGLF"
     gene            complement(3863..4882)
                     /locus_tag="VBC52_001551"
     CDS             complement(3863..4882)
                     /locus_tag="VBC52_001551"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO6883126.1"
                     /translation="MYLIEPIRNGKRITDGAIALAMQVYILQNVFLDDDILFPYYCDP
                     KVEIGKFQNAVIETNQEYLKEHDIPVVRRDTGGGAVYVDSGAVNICYLMKDHGQFGDF
                     KRAYEPAIKALKTLGASSVEMRGRNDLVIDGKKVSGAAMTIVNGRIYGGYSLLLDVDF
                     DAMEKVLNPNRKKIESKGIKSVRSRVGDIRSHLSEDYRHITTDQFKDLMVCQLLHIDH
                     IDQAKRYHLTEKDWAAIDALADEKYKNWDWNYGNSPQYSYHRDARFPSGTYDFHLEIE
                     KGIITNCRIYGDFFSSKDISDIENLLIGCPMKEELVLEKLSTVSLEDYFGQTSPEEIK
                     AVLFP"
     gene            complement(4909..5787)
                     /locus_tag="VBC52_001552"
     CDS             complement(4909..5787)
                     /locus_tag="VBC52_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEO6883127.1"
                     /translation="MTVWKTLEKTNHSQSEILSQLIEESDAIVVGIGAGMSAADGFTY
                     IGPRFEEAFPDFIAKYQLLDMLQASLYDFEDWEEYWAFQSRFVALNYLDQPVGQAYLD
                     LKDILAKKEYHIITTNADNAFAVADYNLEKVFHIQGEYGLWQCSQHCHQQTYRNDQAI
                     RQMIAQQKDMKIPSNLIPKCPKCDQPFEINKRNEEKGMVEDADFHAQRQRYENFLSQH
                     QNDKVLYLEIGVGHTTPQFIKHPFWRFVSLNENSLFVTLNHKHYRIPQKIRSRSVQLT
                     QHIAELIAEAKKEIIG"
     gene            complement(5756..6574)
                     /locus_tag="VBC52_001553"
     CDS             complement(5756..6574)
                     /locus_tag="VBC52_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEO6883128.1"
                     /translation="MPNQKQLLLAMIEYLRSEKLTDVDDLRTTDLQTVWRALVNQQDP
                     QNISQEYLSLEDRYLSHWWNTQKVKTIDVCHQTVYSNVFTYHGDICLLQVDAIVNAAN
                     GKLLGCFIPNHHCIDNHIHTFAGSRLRLACHQLMTQQGRMEAVGQAKLTESYHLPCKY
                     VIHTVGPYVKVDQKPSRIREDLLKSSYKSCLQLAVRANLKTIVFPCISTGEFGFPNQR
                     AAELAVQAILEWQRENQHKLYIIFNTFTPKDQEIYQKLLLKEEIDDSVEDSRKN"
     gene            complement(6567..6899)
                     /locus_tag="VBC52_001554"
     CDS             complement(6567..6899)
                     /locus_tag="VBC52_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEO6883129.1"
                     /translation="MKKIANYLLIEKNEERYTISLTPELQDDLGTVGYVEFTDDTNLE
                     VDDVILNIEASKTVMAILSPLTGKVVKVNTAASQEPTLLNSEKADENWLVVLTDVDYA
                     AFEALENA"
     gene            complement(6928..7914)
                     /locus_tag="VBC52_001555"
     CDS             complement(6928..7914)
                     /locus_tag="VBC52_001555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HEO6883130.1"
                     /translation="MKLSVLDYGLIDYGKTASDAIQETILLSQEAERLGYHQFWVAEH
                     HGVKAFSISNPELMIMHLANQTKSIKIGSGGIMPLHYSSFKLAETLKTLETCHPNRVS
                     IGLGNSLGTVKVSNALRSLHKAHDYEEVLEELKSWLIDESSSKEPLVQPTLSSFPDLY
                     VLGSGQKSAYLAAKLGLGFTFGVFPFMDKDPLTEAKKLSSLYYHQFEEYYPNKSPNLM
                     VAAFVVIADTSEEAENIAKTLDIWMLGNKDFNEFATFPTIEEANHYQLTPEQKAKIKS
                     NRHRMIVGDPKQVKDSLDALVNASQAEELLLIPLVPGLDQRIKSLKLLSQLY"
     gene            complement(7911..9110)
                     /locus_tag="VBC52_001556"
     CDS             complement(7911..9110)
                     /locus_tag="VBC52_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEO6883131.1"
                     /translation="MNNVQGNLFRPLILPNGLSLENRFVLSPMVTNSSTSEGFVTDDD
                     IAYAVRRAKSAPLQITGAAYVTEYGQLFEYGFSVSKDEDIPGLTKLAKAMKSKGAKAV
                     LQLTHAGRFSSHTLARHGYVYGPSPMQLQSPYPHQVKELTHKDILMIIDEYVQATRRA
                     IQAGFDGVEISSAQRLLIQTFFSTFSNQRKDEYGPQTLTNRCRLGLEVFKAVQKVIRE
                     EAESDFILGFRATPEETRGSQIGYSIEEFMEFLEKILAIAQVDYLAIASWGHDVFRNT
                     IRSEGVYKGQLVNQVIFEHFGDRVPIMATGGINSASKVFEALQHAHMVGASTPLVVDP
                     EFLQKIKAKRSDQINLRIKVSDLEGLAIPKASFKDIVPLMDYGESLPKEAREVFRELR
                     SNYREER"
     gene            complement(9103..9939)
                     /locus_tag="VBC52_001557"
     CDS             complement(9103..9939)
                     /locus_tag="VBC52_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEO6883132.1"
                     /translation="MEWQDLAQLPVSIFKDYVTDAQDAEKPFIWTEVFLREINRSNQE
                     IILHIWPMTKTVILGMLDRELPHLELAKKEIISRGYEPVVRNFGGLAVVADEGILNFS
                     LIIPDVFERKLSISDGYLIMVDFIRSIFSDFYQPIEHFEVETSYCPGKFDLSINGKKF
                     AGLAQRRIKNGIAVSIYLSVCGDQKERSRMISDFYKIGLGDTGSPIAYPNVDPEIMAN
                     LSDLLDCPMTVEDVIDRMLISLKQVGFNDRLLMIRPDLVAEFDRFQAKSMANKGTVSR
                     DE"
     gene            10078..10779
                     /gene="coaB"
                     /locus_tag="VBC52_001558"
     CDS             10078..10779
                     /gene="coaB"
                     /locus_tag="VBC52_001558"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEO6883133.1"
                     /translation="MYNMAMKILITSGGTTEKIDTVRSITNHATGALGKIIAEKYLRE
                     GHQVTLVTTKNAIKPESATNLSIFEIEDVDSLIKTLKPLVKEHDILIHSMAVSDYTPV
                     YMADFEKVKSSDDLDTFLRKDNHEGKISSESEYQVLFLKKTPKVISLVKKWNPQIILV
                     GFKLLVNVTKENLFKVARHSLIKNKATFILANDLIDITSKHHIAYLLDHDNVYKATTK
                     EDIAQLIYEKVKKYD"
     gene            10772..11314
                     /gene="coaC"
                     /locus_tag="VBC52_001559"
     CDS             10772..11314
                     /gene="coaC"
                     /locus_tag="VBC52_001559"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEO6883134.1"
                     /translation="MIKRITLAVTGSISAYKAADLTSQLTKIGYDVHIIMTQAATEFI
                     TPLTLQVLSKNPIHLDVMDEHNPKIINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALAMPDETPKLIAPAMNTKMYHNTITQRNIDILKKIGYQEIEPRISLLACGDT
                     GQGALADISTILKCIQEVAN"
     gene            11360..11932
                     /locus_tag="VBC52_001560"
     CDS             11360..11932
                     /locus_tag="VBC52_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6883135.1"
                     /translation="MKKKNKSSNIAIIAIFFAIMLVIHFLSSFIFSFWLVPIKPTLMH
                     IPVIIASIAYGPRIGATLGALMGGISVANSSIVLLPTSYLFSPFVENGNFYSLIIALV
                     PRILIGIIPYFVYKLLHNRFGLAISGAIGSLTNTVFVLSGIFIFFSSTYNGNIKLMLA
                     GIISSNSLAEMVIAAIIVYLTVPRILNIKH"
     gene            12042..13760
                     /locus_tag="VBC52_001561"
     CDS             12042..13760
                     /locus_tag="VBC52_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO6883136.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSAGLIQVIGEDVDIEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVDADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQTATKNDG
                     IISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATVGNDLADAETKIANIEKE
                     ITTFVN"
BASE COUNT         4481 a         2691 c         2197 g         4509 t
ORIGIN      
        1 aaattttaca aagaatgtga tggtagcata ttcaattaaa gttaagtaaa gacataattt
       61 taagaaggaa attgtgactt atttatgcaa aaaagaaaga accagtttgg ttctttcttt
      121 tttgcttttc agctacttac cacaatttgt aagaaattac aagtagtagt aagctaaggg
      181 caccttttca agtgtgacaa gaaggttcac acaacttatc aagatccgct cctgcgttta
      241 taatctccct gcgcaatagt ggtaaaacca catatcgact catcgcataa cagtagttta
      301 acataattta gttaaaatgc aacctttact ttttaaatat aactagattc ttcagtttac
      361 ttttaatatt aaattaaaaa taaattattt ctaaagcagg ggagcaatag tttttgtcac
      421 tatgccaatt actttttgga attttggaat attatttata tctttcaagc taactaacct
      481 tgatttattt aatgtatcca tataatctcg ataaatgtct tggattgcat ctaccttata
      541 aagataaaca gcacattcaa aatggtgata caggctacgg taatcaagat ttatcgtacc
      601 tacaacagcc tttgtattat cactaaggaa tatttttgaa tgaacaaatc caggggtgta
      661 ttcatatatt ttaacacctg attttgttaa agcttgatag taagttttag caagagcata
      721 agggattggc ttatctggga tacccggcat aataatacgt acatctacac tacgttccgc
      781 agcaaactga atggcatgtt ccaattcact gtctaatatt aaataaggtg tcatgatgta
      841 aacatattct ctagcatgat taagaatatc tatataaaca ttttccccta ctttatcagt
      901 gtctaaagga gaatctccat aaggaataac atagccttca ttttcaacta aatcatcgtg
      961 tacaccgagg taaggcgcta ctaacatttt ttcctcagta atggaccaca tctgtaagaa
     1021 aagtaggagg aaactatcca ctgctttccc ctttaacata atacctgaat ctttccaatg
     1081 cccaaataac tcaatgtgat taatatactc atcggctaag tttacaccac cagtcatgcc
     1141 tactactcca tcaataacaa caatttttcg atggtcacga tagttataat aagttgaaat
     1201 aaaaggagaa atcggagaaa atgcttttgc ttttattcct attttctcaa gtcgtttcgt
     1261 ataatcaaag gataattttg taatttctat cataccatca tataagatac ggacttcaac
     1321 accttcttcc accttttgtt ctaaaatact aagtatttct ccccacatta ttccttcgtc
     1381 aatgataaag aattctaaaa agatatactt tttagctttt agtagttctt ctttcatttt
     1441 ttcaaagaat gcttctcccg ttggtagaaa tgtcacatca gtatttcgat aaactgggaa
     1501 atttccatgt gctttttcaa aatattgaac caaatgatat gttgtggatg tacttgactt
     1561 aagctgattc aaagtttcag gatcatcctc tagataaatc tgactctcat caatcaatgt
     1621 ctccaatctt tgtttcaaac cacgataacc ccagtcaaat ttggtgtaca ttaggaacat
     1681 cgcccctagt aaaggggcaa tcattaccaa tattaaccaa gttactcggg acaaagcatc
     1741 catttcacta ttaactaaat ataagaccgc tccaattgtt aagatatgct caacggttgc
     1801 taaccaaaca cgatactgct ctagccatga gtaagaggct agtaaaaaca ataattgtaa
     1861 aatcaataaa atagcgataa cggttgttcg actaaatata ccccttagta aacttttctt
     1921 actcttatta agtaaacgaa caattttgac tttattactg ataagttttc ccccctgcaa
     1981 atcaatcatt ttattatagc atactgtcac ttgaatagca ttctattatt ctaaattatc
     2041 tctagttaaa atctaatccc caataaaaag aggctagcat cacaagccct acctctttat
     2101 tatttaaaat aatccaatgg ctgtaccgtc ttctaaaaca tccatattga gagctgcagg
     2161 ttttttaggt aaaccaggca ttgtcaatac gtctccagta agagcaacta taaaaccagc
     2221 tcctgttttt ggaacaaatt cacgaacagt tatatcaaag tcagttggag caccaagtaa
     2281 atttggatta tcagaaaagc tatattgtgt ctttgccata caaataggca tcttgtccca
     2341 gccattgtca ctaaactctt ttaattgtat ttgtgcctta ggtccaaaat gaactttatt
     2401 accaccatat attttagtaa caattttttt aattttttct tcaatagtgt cttcatcatt
     2461 atataagcgt ttgtaattgg atgattgtgt ttcaatcaca ttagcaacag tctgtgcaag
     2521 ttcaaggcca ccgtctgctc catcttccca cacacttgct aattcaactg ccacatcaat
     2581 attactacat aaggttttaa gagttgcaat ttcactttct gtatctgcag taaactgatt
     2641 gatagctaca actaccggaa caccatattg acgcatattg ttaacgtgac gctcgagatt
     2701 tgtaaaacct cttttaactg ctttgacgtt ctcttgtgaa agatcttctt ttgaaacacc
     2761 tccatgcatt ttcaatgcac gcaatgtagc aacaataaca atcgcatcgg gagaagtcgg
     2821 aaggtttggc gtttttatat caagaaactt ttcagcgccc aagtctgctc caaaaccagc
     2881 ttcagtaaca acgtaatctg ctaagcgtag agctgttgaa gtcgctaaga cagaattaca
     2941 tccatgtgca atattagcaa atggtccccc atggacaaga gccggtgtcc cataaatggt
     3001 ttgaaccaaa ttaggtttga tagcatcttt taggataagt gtgagagctc cttcaatctt
     3061 caaatcttta acatagattg gtttacggtc ccttgagtaa gccacaacaa tatttgacag
     3121 gcgtttcttt aaatctgata ggtcagtagc taaacataga atagccatta tttcagaagc
     3181 aacagtaata tcaaagccat cttcacgtgg tatcccattg acagggcttc ctaagccaac
     3241 gatcacctga cgaagagcac gatcgttaag atcaactaca cgcttccaaa tcactcgacg
     3301 ttggtcaata tctaattcat tgccttgatg aatatgatta tcaagtagag ctgataaagc
     3361 attattagca gttgtgattg cgtgcatatc accagtaaaa tgcaaattaa tatcttccat
     3421 tggtagtact tgtgcgtatc cacctccagc agctcctcct ttaataccca ttacaggacc
     3481 taaagaaggt tcgcgaagcg caatcatcgt ttttttacca attttattta aagcatcagc
     3541 taatccaatg ctcatagtgg attttccctc acctgctggt gtcggattaa tagcagttac
     3601 taaaattaac tttcctactt tttgagatct tacagcttca atcttatcaa aagataattt
     3661 tgctttgtat ttaccataca attctatatc atcaaaacca attcctactt gctcaacaat
     3721 ctcagcaata ggctttaggg cgacgctttg cgctatttca atatctgttt tcatgatatt
     3781 ctccttctaa ataataaaaa tagtatagca atattcccta taaaaacata tttttttgca
     3841 aaaaaaattt aaaatgttcg tcttatggaa ataaaactgc ttttatttct tctgggctag
     3901 tctgaccaaa ataatcttct agtgatacag tacttaattt ttccaagact aattcttctt
     3961 tcatagggca accaatgaga aggttttcaa tatctgaaat atctttagaa ctgaaaaagt
     4021 caccataaat acggcaattc gtaataatac ctttctcaat ttctaagtga aaatcgtaag
     4081 ttcctgacgg aaaacgagca tctctgtgat acgaatactg tggtgagtta ccataattcc
     4141 aatcccaatt cttatatttt tcatcagcta gagcgtcaat cgcagcccaa tctttttcgg
     4201 ttagatgata gcgttttgcc tgatcaatat gatcaatatg taataattga caaaccatta
     4261 aatctttaaa ttgatcggtt gtgatatgac gataatcttc tgacaagtgg ctacgaatat
     4321 ctcctactcg acttcggaca gattttatcc ctttagattc aatttttttc cgattaggat
     4381 ttaacacttt ttccatagca tcaaaatcaa catctaataa aagagagtaa ccaccgtaaa
     4441 tacgaccgtt tactatcgtc attgctgcac ctgatacttt tttaccatca ataactaagt
     4501 catttcgtcc cctcatttca acactagaag cacctaaagt ttttagggcc ttaatagctg
     4561 gctcataagc tcgtttgaaa tcaccaaatt gtccatgatc tttcattaga tagcaaatat
     4621 ttacagcacc tgagtcaacg taaacagccc ctccaccagt atctcgacga acaacaggaa
     4681 tatcatgttc tttcaaatat tcttgattag tctctataac tgcattctga aattttccga
     4741 tttcaacttt aggatcacaa tagtatggaa ataaaatatc atcatctaaa aaaacatttt
     4801 gtaaaatata aacttgcatg gcaagtgcaa tcgcaccgtc tgtgatacgc ttaccatttc
     4861 taattggttc aataagatac atttaggtct ccttttatag caatttagtt atccaataat
     4921 ttcttttttc gcttcagcaa ttaattcagc gatatgctgt gtcagttgca cactccttga
     4981 gcgaatcttt tgaggaatgc gataatgctt gtgattaaga gtaacaaata aagagttttc
     5041 atttaaagag acgaatcgcc aaaacggatg cttaataaat tgtggcgtgg tatgtccaac
     5101 tccaatctct agataaagaa ctttgtcatt ttgatgttgt gataagaaat tttcataacg
     5161 ttgtcgttga gcatgaaagt cagcatcttc caccatacct ttttcttcgt tacgtttatt
     5221 aatttcaaac ggttgatcac actttgggca tttggggatt aagttactag gaatcttcat
     5281 atccttttgt tgtgcaatca tttgacgaat tgcctgatca tttcgataag tttgttgatg
     5341 acaatgttga ctacactgcc ataagccata ttcaccttga atatgaaaaa ctttttctaa
     5401 attataatca gccacagcga aggcattatc agcattagtt gtaataatat gatattcttt
     5461 tttcgctaaa atatccttta aatcaagata agcttgtcct accggttgat ctaaataatt
     5521 aagtgccaca aaacgacttt ggaaggccca atattcttcc caatcttcaa agtcataaag
     5581 acttgcttgc aacatatcta atagttgata tttagcaata aaatccggaa aagcttcttc
     5641 aaatctgggg ccaatatagg tgaatccatc cgctgcagac atgcctgcac caataccaac
     5701 tacaatagca tctgattctt ctattaactg acttagtatt tctgattgag aatgattagt
     5761 tttttctaga gtcttccaca ctgtcatcta tttcctcttt taataataat ttttgataaa
     5821 tctcttggtc tttaggtgtg aatgtattga aaataatata gagtttgtgc tgattttcgc
     5881 gttgccactc taatatcgct tgaacagcca attcagcagc tctttgatta ggaaaaccaa
     5941 attctcccgt tgatatacaa gggaagacaa ttgttttaag attagctcga actgccaact
     6001 gtaagcagga tttataagaa gactttaata aatcctctct tatacgagat ggcttttgat
     6061 caacttttac gtaaggtccg actgtatgaa taacatactt acaaggaaga tgataggatt
     6121 cggtaagttt agcttgtcct acggcttcca ttcgtccttg ttgtgtcatc aattgatgac
     6181 atgctaaacg taatcgtgaa cctgcaaacg tatgaatatg attgtcaata caatggtgat
     6241 taggaataaa acaacctaac aacttaccat ttgctgcatt aacaatagca tccacttgta
     6301 ataagcaaat atccccatgg taggtaaata catttgaata taccgtttga tgacagacgt
     6361 ctatcgtttt tactttttga gtattccacc aatgtgacaa atacctatct tctaaagaca
     6421 ggtactcttg actaatgttt tgaggatctt gttgattaac tagagcacgc caaaccgttt
     6481 gcaaatcggt ggtcctcaag tcatcaacgt ctgttaattt ttctgaccga agatactcaa
     6541 tcatggctag caatagttgt ttttgattag gcattttcta aagcttcaaa agcagcataa
     6601 tcaacgtctg ttaacacaac caaccaattt tcgtcagctt tttcagaatt taacaaagtt
     6661 ggttcttgac ttgcagctgt attaacttta acaactttac ctgtaagagg agataaaatt
     6721 gccataaccg tctttgaagc ttcaatattt aaaataacat catcaacttc taaatttgta
     6781 tcatctgtga attctacata tccaacagtt cccaagtcat cttgaagttc gggagttaag
     6841 ctgatagtat aacgttcttc atttttttca attaataagt agttcgcaat ttttttcatg
     6901 agtattttcc tttctgagaa aacgtgatta atataactgt gataataatt ttaaggattt
     6961 gattcgttga tcaagtcctg gaacaagagg aatcaaaagt agttcttctg cttgactagc
     7021 attaactaaa gcgtcaaggc tgtctttaac ttgttttgga tcacctacaa tcatacggtg
     7081 gcgatttgat ttaatttttg ctttttgttc aggagtcaat tggtaatgat tagcttcttc
     7141 aatagtagga aatgttgcaa attcattgaa gtctttattt cctagcatcc agatatctaa
     7201 agtctttgct atgttttcag cttcttctga agtatcagct ataactacaa atgcagctac
     7261 catcaaatta ggagacttat taggataata ttcttcaaac tgatgataat aaagagatga
     7321 aagcttttta gcttctgtca atgggtcttt gtccataaaa ggaaaaacac cgaaggtaaa
     7381 gcctaagcca agtttagccg ctaaataagc tgatttttga ccactcccca acacatataa
     7441 gtctgggaag ctagaaagag tcggttgaac taatggttcc ttactggatg attcatcaat
     7501 aagccatgac ttcaattcct ccagtacctc ttcgtaatca tgtgctttat gtaagctacg
     7561 aagtgcattt gaaactttaa ctgtccctag tgaatttcct aaaccaatac ttactcgatt
     7621 cggatgacat gtctctaatg tcttgagagt ctccgcgagt ttaaaactac tatagtgcag
     7681 aggcattata cctccagagc caattttgat agatttagtc tggttagcca aatgcattat
     7741 cattaattct ggattgctaa tactgaatgc cttaacaccg tgatgttcag ccacccaaaa
     7801 ttgatgatat cctagtcgct ccgcctcttg tgataaaaga atcgtttctt gtattgcatc
     7861 acttgcagtt tttccataat caataagccc ataatcaagg acacttaatt tcatctttcc
     7921 tccctataat tgcttcgaag ttctctaaaa acttctcgag cttcctttgg taaagattct
     7981 ccataatcca tcaaaggaac aatatcttta aaggaggctt ttggaatcgc caaaccttct
     8041 aaatcactca ctttaatcct taaattaatc tgatcagaac gcttagcttt tattttttgt
     8101 aaaaattccg gatcaactac taagggcgtg gaagctccta ccatatgagc atgttgtaag
     8161 gcttcaaaca cctttgaagc actattaata cctccagttg ccataattgg aactctatca
     8221 ccaaaatgtt caaatataac ttgattaacc agctgccctt tgtaaactcc ctcagaacgt
     8281 atagtatttc taaagacatc atgcccccag cttgcaatag caagataatc tacctgagca
     8341 atcgctaaaa ttttttcaag aaattccata aactcttcaa tgctatatcc aatttgtgaa
     8401 ccgcgagttt cttcaggcgt cgctctaaaa ccaaggataa aatctgactc tgcttcttct
     8461 ctgataactt tttgtaccgc tttaaaaact tctaagccta aacgacagcg attagtcaaa
     8521 gtttgaggac catactcatc ttttctttga tttgagaagg ttgagaaaaa agtttgaatt
     8581 agtaatcgtt gtgcagaaga tatttcaaca ccatcaaatc cagcttgtat agctcgtctt
     8641 gtagcctgga catattcatc gataatcatt agtatatctt tgtgtgttag ttctttaacc
     8701 tgatgtgggt atggcgattg caattgcata gggctgggac catatacata tccgtgtctt
     8761 gccaaagtgt gacttgagaa acgtccagca tgcgtcagct gcaaaacagc tttcgctccc
     8821 ttagacttca ttgctttagc caatttagtc aaaccaggaa tgtcctcatc ctttgaaacg
     8881 ctaaaaccat attcaaataa ctgaccatat tctgtaacat atgctgctcc agtaatttgt
     8941 aatggagcgg atttagcacg acgaacagca tatgctatgt cgtcatcagt tacaaaacct
     9001 tctgatgtag aagaattagt taccataggt gatagaacaa aacgattttc taagctcaat
     9061 ccattgggaa gtattaatgg acgaaataaa ttaccttgga cattattcat ctctgctcac
     9121 cgtcccctta ttagccatag acttagcctg aaatctatca aactctgcaa ctaaatcggg
     9181 cctaatcatc agtaaacgat cattaaaacc tacttgttta aggctaatca acatacgatc
     9241 aataacatct tctactgtca taggacaatc taatagatca gatagattag ccataatttc
     9301 aggatctaca tttggataag caattggact acccgtatca cctagaccaa tcttataaaa
     9361 atctgaaatc attcgactcc gctctttttg atcgccacaa acgctaaggt aaatcgatac
     9421 cgcaatacca ttctttatac ggcgctgagc caagccagca aattttttgc catttatact
     9481 aagatcaaac ttaccaggac aataggaggt ctctacttca aagtgctcaa taggttgata
     9541 aaaatctgaa aatatacttc taataaaatc gaccattata agatacccat ctgagataga
     9601 caattttctc tcaaaaacat ctggaataat caatgaaaaa tttaaaattc cttcatcagc
     9661 tacaactgcg agaccaccaa aattccgaac aactggttca tagccacgac tgatgatttc
     9721 ttttttagct aattctaaat gtggtaattc tcgatctaac atccccagaa tgactgtctt
     9781 agtcatcggc caaatatgca aaataatttc ttgatttgag cgattaatct cccttaaaaa
     9841 tacttctgtc catataaaag gtttttccgc gtcttgagca tctgtaacat agtctttaaa
     9901 aatagataca ggtaactgcg ctagatcttg ccattccaat aaatctgacc tcctttctct
     9961 agaaaactct gcaagattta ttttatcgca atcgcttgaa attttccagt aagtttccct
    10021 caaattccat tactatcaag aagaaatata tcttttattt acgaaaagca tttttttatg
    10081 tacaatatgg ctatgaaaat tttgattaca tcaggtggaa ctaccgagaa aattgatacc
    10141 gttaggagta tcacgaatca cgcaacagga gctttaggaa aaataattgc agaaaaatat
    10201 ttacgagaag gacatcaggt gacactcgtg actactaaaa atgcaatcaa accagaatct
    10261 gctaccaact tatccatatt tgagattgag gacgttgact cattaataaa aaccttgaag
    10321 ccattagtga aagagcatga tattttaatt catagcatgg cagtgtctga ctataccccc
    10381 gtctacatgg ctgatttcga aaaggttaaa tcaagcgatg atttagatac ttttttgcgc
    10441 aaagataacc acgaagggaa aatatcatct gaatcagaat atcaagtcct ttttctcaaa
    10501 aaaacaccaa aagttatcag ccttgtaaaa aaatggaatc ctcaaattat tctagtaggt
    10561 tttaaactac ttgtgaacgt tactaaagaa aatcttttta aagttgctcg tcatagctta
    10621 atcaaaaata aagctacgtt tattttagct aatgacctca ttgatattac ctcaaaacat
    10681 cacatagcct acctacttga tcatgataat gtgtataaag ctaccaccaa agaagacatt
    10741 gctcaattaa tttacgaaaa ggtaaaaaaa tatgattaaa cgtattacac tagccgtcac
    10801 cggttcaatt tcggcctata aagcagcaga ccttacaagt caattaacta aaattggcta
    10861 tgatgttcat atcataatga cacaagctgc cacagaattt attacaccgt taactctgca
    10921 agtattatca aaaaatccta ttcatttaga tgttatggat gagcataacc caaaaatcat
    10981 caaccatatc gaattagcaa aacgcacaga tttatttatc gttgcacctg cctctgctaa
    11041 tactattgct catctagcat atggcttcgc tgataacatc gtaacaagcg ttgctcttgc
    11101 tatgcctgat gaaactccaa aacttatcgc accagcaatg aatacaaaga tgtatcataa
    11161 tacaatcacc caacgtaaca ttgatatttt gaaaaaaata ggttatcagg aaattgaacc
    11221 tcgtattagt ttgcttgcct gtggtgatac cggtcaagga gccctagctg atatctctac
    11281 tatacttaaa tgtatacaag aagttgcaaa ttaggctgta aatttgtata ctatttctag
    11341 tttaatttaa ggagttataa tgaaaaagaa aaacaaatcc tctaacattg ctataattgc
    11401 aatctttttt gctattatgc ttgtcattca ttttttgtca tcatttattt ttagtttttg
    11461 gttagtcccc attaaaccta ctttgatgca tatcccagtt attattgcat ctatagccta
    11521 tggacctcgt attggtgcaa ctctaggcgc cttaatgggt gggatcagcg tagctaacag
    11581 cagcattgtt ctattaccaa cgagttacct cttctcacct tttgttgaaa atggtaattt
    11641 ttattcgcta attattgcac ttgtaccacg tattctaatc gggattattc cttatttcgt
    11701 ttacaaatta ctacacaacc gctttggttt ggctatctca ggtgctatag gctctctaac
    11761 aaacacagta tttgttttat ctggaatttt tatctttttt tcaagtactt ataatgggaa
    11821 tatcaagcta atgctcgctg ggattatttc atctaattca ttagctgaga tggtcattgc
    11881 agctatcatt gtatatctaa ctgttcctcg tattctcaat attaaacatt aaaaactggc
    11941 caggaagaaa tcttcctggc ttttcttact tatattttat agtcatattg aaaaaaacat
    12001 gttataatga aagcgtttat aaatattgaa ggagtatttc aatgacttac acagaaaatt
    12061 ttcaaaaatg gttagatttt gagcagcttc ctgactatct tcgtcaagaa ttactatcaa
    12121 tggatgaaaa aacaaaagaa gatgcttttt acacaaactt agaatttggt actgctggta
    12181 tgcgtggtta tattggtgct ggtacaaatc gtattaatat ttatgttgtc cgtcaagcaa
    12241 ccgaaggatt ggcaaaatta atcgaaacta agggagagga agctaaaaaa cgtggtgtag
    12301 ctatcgctta tgattcgcgc cacttctctc cagaatttgc ttttgagtct gcacaggtct
    12361 tggcacagca tggaattaaa tcttacgtat ttgaagctct tcgtccaaca ccagaactct
    12421 cttttgctgt tcgccattta aatgcctatg ctggtattat ggttactgca agccacaatc
    12481 cagcaccttt caatggttat aaagtctacg gacaagacgg aggtcaacta ccaccagctg
    12541 atgctgatgc actaacagac tttatccgtg ctattgaaaa tccatttgca gttgaacttg
    12601 ctgatttaga tgaaagcaaa tcagcgggtc ttattcaagt tatcggtgaa gatgttgata
    12661 ttgaatatct tagagaggtt aaagatgtta atatcaatca agatttaatc aataactttg
    12721 gaaaagacat gaaaattgtc tatactcctc tccacggaac tggtgagatg ttgacacgcc
    12781 gtgcccttgc tcaagccggt tttgaatctg ttgttgtagt agaatcacaa gctaaggcag
    12841 atccagactt ttctactgtc aaatctccta accctgaaag ccaagctgcc tttgctcttg
    12901 ctgaagaact cggacgagaa gttgatgcag atgtcttagt agctactgat cctgatgcag
    12961 accgcctagg cgttgaaatt cgccaaccag atggttctta taaaaacctt tctggaaatc
    13021 aaattggagc gattattgcg aaatacatcc tggaagctca taaaacagca ggaacacttc
    13081 cagaaaatgc tgctctcgct aaatcaatcg tatcaacaga attagtaaca aaaattgccg
    13141 aaagttatgg cgctacaatg tttaacgttt taactggatt taaattcatc gctgagaaaa
    13201 ttcaagagtt tgaagaaaaa cataaccaca cctatatgtt tggttttgaa gaaagcttcg
    13261 gatatcttat taaaccattt gtccgtgata aagacgctat tcaagcagtt cttttggttg
    13321 ctgagattgc agcttattac cgttcgcgtg gtttaacact tgcagatggt attgatgaaa
    13381 tttacaaaga atatggttat tttgctgaaa aaacgatttc agtaacactt tcaggagtgg
    13441 atggagctgc tgaaattaag aagattatgg acaaattccg tgaaaatggt ccaaaacaat
    13501 ttaacaatac tgatattgta ttattagaag atttccaaaa acaaaccgct actaaaaatg
    13561 atggaataat atctaactta acaactcctc caagtaatgt tttgaagtac acgttagctg
    13621 atgattcatg gattgcagtt cgcccttcag gaactgaacc aaaaatcaag ttctatattg
    13681 ctacggtcgg taatgattta gcagatgcag aaacaaaaat cgccaatatt gaaaaagaga
    13741 ttacaacatt tgtaaattaa taaaaaaagc taggaactta ctcctagctt tttttatatc
    13801 tatctaacga tgttctatta ttttcaatta tatgaaatat aactgcaatt aacttcacga
    13861 tcaaataata gagcagac
//
LOCUS       DATRNK010000054        13261 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.054, whole genome shotgun sequence.
ACCESSION   DATRNK010000054 DATRNK010000000
VERSION     DATRNK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13261
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.054"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            159..1229
                     /gene="xerS"
                     /locus_tag="VBC52_001562"
     CDS             159..1229
                     /gene="xerS"
                     /locus_tag="VBC52_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEO6883137.1"
                     /translation="MKRELLLEKIDELKEIMPWYVLEYYQSKLSVPYSFTTLYEYLKE
                     YRRFLEWLLDSGVANCHHIAEIELSVLENLTKKDMEAFILYLRERPLLNANTRQNGVS
                     QTTINRTLSALSSLFKYLTEEVENADGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGNETIEFLEYINCEYQNKLSKRALAFFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LKDINLNVMVIDVTRKGGKRDSVNVASFAKPYLANYLDIRKNRYKAENQDIALFLSEY
                     RGVPNRIDASSVEKMVAKYSQDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDKL"
     gene            complement(1272..2210)
                     /locus_tag="VBC52_001563"
     CDS             complement(1272..2210)
                     /locus_tag="VBC52_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6883138.1"
                     /translation="MIRQFLREHLIWYILYIMMFVLFFISFYLYHLPMPYLFNSLGLN
                     VIVLLGISIWQYSRYRKKMLHLKYFNSSQDPSFELQPSDYAYFNIITQLEAREAQKVS
                     ETIEQTNHVALMIKMWSHQMKVPLAAISLMAQTNHLDPKEVEQQLLKLQHYLETLLAF
                     LKFRQYRDDFRFEAVSLREVVVEIIKSYKVICLSKSLSIIIEGDNIWKTDKKWLTFAL
                     SQVLDNAIKYSNPESKIIISIGEESIRIQDYGIGILEEDIPRLFEDGFTGYNGHEHQK
                     ATGMGLYMTKEVLSSLNLSISVDSKINYGTAVSIHK"
     gene            complement(2207..2875)
                     /locus_tag="VBC52_001564"
     CDS             complement(2207..2875)
                     /locus_tag="VBC52_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6883139.1"
                     /translation="MSQEQGKIYIVEDDMTIVSLLKDHLSASYHVSSVSNFRDVKQEI
                     IAFQPDLILMDITLPYFNGFYWTAELRKFLTIPIIFISSSNDEMDMVMALNMGGDDFI
                     SKPFSLAVLDAKLTAILRRSQQFIQQELTFGGFTLTREGLLSSQDKEVILSPTENKIL
                     SILLMHPKQVVSKESLLEKLWENDSFIDQNTLNVNMTRLRKKIVPIGFDYIHTVRGVG
                     YLLQ"
     gene            complement(2985..4940)
                     /locus_tag="VBC52_001565"
     CDS             complement(2985..4940)
                     /locus_tag="VBC52_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6883140.1"
                     /translation="MFYLKIAWHNLKHSIDQYIPFLLASLLLYSLTCSTLLILMSAVG
                     RDMGTAATVLFLGVIVLSIFAVVMEHYSYNILMKQRSSEFGLYNILGMNKRQVARVAS
                     LELFIIYIFLISIGSLFSAFFAKFIYLIFVNIINYHALNLSLSLWPFIICIVIFTGIF
                     LTLEVPVIRHVHLSSPLSLFRKKQQGEKEPKGNLILAILALVAIAIAYTMALTSGKAP
                     ALAVIYRFFFAVLLVIAGTYLFYISFMTWYLKRLRQNKHYYYKSEHFVSTSQMIFRMK
                     QNAVGLASITLLAVMALVTIATTVSLYSNTQNVVTGLFPKSVSLSIDNSKGDAKNIFE
                     EKILKKLGKSSKEAITYNQTMISMPVSQSSELNITSKNVKHVDITKTGFMYLITQNDF
                     RRLGHQLPKLKDNQVAYFVQKGDSRLKKINLLGNKFDVVKNLKEAYVPETTNTYNPGL
                     IIFANNKQIDNIRKAYLPYTKNINTFPKTFKAYLDLNSQEINSISKNDIIEVDGKYVG
                     NISTKQSFLKEGYQMFGGLLFTGFLLGISFLLGIALIVYYKQYSEGHEDKRSYRILQE
                     VGMSKKLVKRTINSQIMIFFFQPLVVAVIHFGVAIPMLKQMLLVFGVLNSTIVYVVSG
                     LTVLAISIIYFIIYRITSRTYYHIIER"
     gene            complement(4942..5694)
                     /locus_tag="VBC52_001566"
     CDS             complement(4942..5694)
                     /locus_tag="VBC52_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6883141.1"
                     /translation="MLLEINHLEKVFRTRFSKEETRALQDVDFKVEQGEFIAIMGESG
                     SGKTTLLNILATLEKPTNGQVILNGEDITKIKEAKLASFRLKNLGFVFQDFNLLDTLS
                     VRDNIYLPLVLDRKRYKEMDHRLSELSSHLRIDDLLDKRPFELSGGQKQRVAIARSLI
                     TNPQILLADEPTAALDYRNSEDLLNLFETINLDGQTILMVTHSANAASHAKRVLFIKD
                     GRIFHQLYRGNKNNSEFNKDISLTMSAILGGE"
     gene            complement(5720..6681)
                     /locus_tag="VBC52_001567"
                     /pseudo
     CDS             complement(5720..6681)
                     /locus_tag="VBC52_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253131.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
     gene            6847..7335
                     /locus_tag="VBC52_001568"
     CDS             6847..7335
                     /locus_tag="VBC52_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEO6883142.1"
                     /translation="MAKTSKKLTKSLGPIGKLISIIPDTTELIGKAIDNSRPIIEKEL
                     DRRHEKKQTYTQLDNVVHLDINDAQVYLEQRHFNVSRIVAEPNSKWATLHPNQVVNMS
                     PKAGRVRIGSLVKLYYITEDILEESKTLLLSKSEKSKARQQRLTEVLSTPSQLLKKNR
                     NK"
     gene            complement(7367..8215)
                     /locus_tag="VBC52_001569"
     CDS             complement(7367..8215)
                     /locus_tag="VBC52_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO6883143.1"
                     /translation="MKIKKLLGLTTTVVISALILGACGQSKNEDAKVVRVGTMVKSKT
                     EKARWDKIEELVKKKGVKLKFTEFTDYTQPNKALESDEIDINAFQHYNYLNNWNKANK
                     TNLVSVAETYFTSFRLYSGTKNGKGKYQTVSEIPNKATITIPNDAVNESRSLYLLQSA
                     GLLKLKVSGDALATMSDVVSNPKSLDLKEVDAAQTARSLDSTDAAVINNDFVTEAGIN
                     PKSAIFIEPKSKNAKQWYNLLVAQKGWQDKSKAKAIKEVVKAYHTDAVKKVIEKTSQG
                     LDQPVW"
     gene            complement(8348..9091)
                     /locus_tag="VBC52_001570"
     CDS             complement(8348..9091)
                     /locus_tag="VBC52_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6883144.1"
                     /translation="MHYKELLESSEKAYLETNRYKHFKNIIISERYFSNYISYKIMPT
                     VSEFLQDMIYLADEQKDYQSDFAFVFFEENAELSSELQRTLSQQGFESEKHIIFTNSL
                     NHLKLFEKEFDDITFTKLNENYLEKYLKFKYKQSIVYGEIYAKQMYNDNRVNLLNNNA
                     KVYLAIKDNQIIGDVTAWDYGEYVEIDDFYVQESYRGQGIGTRLQFDASRGFKKVILI
                     SEEENRKMYEHQGYEEVAYYWTALRSPRK"
     gene            complement(9169..10503)
                     /gene="trmFO"
                     /locus_tag="VBC52_001571"
     CDS             complement(9169..10503)
                     /gene="trmFO"
                     /locus_tag="VBC52_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEO6883145.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEAHRVPAGGAMAVDREGY
                     SEAVTEEIHKHPLIEVIRDEITDIPGDAITVIATGPLTSDSLAAKIHELNGGDGFYFY
                     DAAAPIVDKNTIDINKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPEDYKGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLNQT
                     FATRKNPNLFFAGQMTGVEGYVESAASGLVAGINAVRRFNGESEVVFPQTTAIGALPH
                     YITHTDSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIATRALKDLEKFLNY"
     gene            complement(10617..11315)
                     /locus_tag="VBC52_001572"
     CDS             complement(10617..11315)
                     /locus_tag="VBC52_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6883146.1"
                     /translation="MLPAYIKIHDAIKKEIDKGTWKIGQRLPSERDLADDYSVSRMTL
                     RQSITLLVEEGILERRVGSGTYVASHRVQEKMRGTTSFTEIVNSQGRKPSSKLISFQR
                     KLANETEIQKLNLSQSDYVVRMERVRYADKVPLVYEVASIPENLIKGFEQSEVTEHFF
                     KTLTENGYEIGKSQQTIYARNASERVASHLEVNAGHAILALTQVSYFTDGKPFEYVHG
                     QYVGDRFEFYLENN"
     gene            11528..13090
                     /gene="guaA"
                     /locus_tag="VBC52_001573"
     CDS             11528..13090
                     /gene="guaA"
                     /locus_tag="VBC52_001573"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEO6883147.1"
                     /translation="MTDISILNDIQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TADEIRDINPIGIVLSGGPNSVYADGAFGIDEEIFELGIPILGICYGMQLITHKLGGK
                     VLPAGEAGHREYGQSALRLRSESALFAGTPQEQLVLMSHGDAVTEIPEGFHLVGDSVD
                     CPFAAMENTEKQFYGIQFHPEVRHSVYGNDILKNFAVNICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQRAIGDQLTCIFVDHGLLRKNEGDQVMDMLG
                     GKFGLNIIRVDASKRFLDLLSGVEDPERKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGTALGMPDEV
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
BASE COUNT         4287 a         2374 c         2110 g         4490 t
ORIGIN      
        1 ataagtaata ctgatcatca taaagaatgg atatttgata tacttaccga atgaattcct
       61 gtttttaact tttaaacttt ccgaaaaact ataattttct tgaaagttat gatagagtat
      121 gatatacttc tatttataga tataatggag attttattat gaaacgtgaa ttactactcg
      181 aaaaaatcga tgaactaaaa gaaattatgc cttggtatgt tctagaatat tatcaatcaa
      241 agctttctgt tccttatagt ttcacaacac tctacgaata tttaaaagaa tatagacgtt
      301 ttcttgaatg gttattggat tctggtgttg ctaattgtca tcacattgca gaaattgagc
      361 tttcagtttt agaaaacctt actaaaaaag atatggaagc ttttatacta tatttacgag
      421 aacgcccttt attaaatgct aacacaaggc aaaatggggt gtctcaaacc accattaaca
      481 gaactctatc tgccttatct agtcttttca aataccttac tgaagaggtt gagaatgctg
      541 acggggagcc ttacttttat cgcaatgtca tgaaaaaagt ctcaaccaaa aagaaaaagg
      601 aaactttagc ttctcgggca gaaaatatta aacaaaagtt atttttgggc aatgaaacga
      661 tagaattctt agagtatata aattgtgaat atcaaaacaa actttccaaa cgtgctttag
      721 cttttttcaa caaaaataaa gagagagatt tagctattat tgctttgcta ctagcttcag
      781 gagtgcgtct atctgaggca gtaaacttag atttaaagga tattaacctt aatgtaatgg
      841 ttattgatgt cacacgaaaa ggggggaaaa gggactctgt caatgtcgct agctttgcga
      901 agccctacct tgctaattat ttagacatcc gtaaaaatag atacaaagca gaaaatcagg
      961 acattgctct atttttatca gaataccgtg gggtgccaaa tcgtattgat gcttcaagtg
     1021 tagagaaaat ggttgctaag tattcccagg attttaaggt tcgtgtaaca cctcataaac
     1081 taaggcatac tcttgctact aggctatatg atgctacaaa atctcaagta ttagttagcc
     1141 accaactagg tcatgcttcc actcaagtta ctgacctata tacccatatt gttaacgatg
     1201 aacaaaagaa cgctttagat aaactatagt aacataaata aagttatgta aatattttat
     1261 tttacataac tttatttatg tatagaaaca gcagtcccat aattaatttt gctatccacc
     1321 gaaatggaca aattcagact agataagact tcttttgtca tatataaccc catgcctgtt
     1381 gccttttggt gctcatgacc gttgtaaccc gtaaagccat cttcaaaaag tctagggata
     1441 tcttcttcga gtatgccgat accgtagtct tgtattctaa tactctcttc tcctatgctt
     1501 attattatct ttgactcagg attagaatat tttatggcat tatctagcac ctgtgaaaga
     1561 gcaaaagtta accacttttt gtctgttttc cagatattat cgccttcaat tatgatagat
     1621 aagcttttgg atagacaaat aaccttatac gattttataa tttctactac tacttctcta
     1681 aggctaacag cttcaaaacg aaaatcgtca cgatattgtc taaatttcaa aaatgctaac
     1741 aacgtttcaa gataatgttg caatttcaat aattgttgtt caacttcctt aggatcgaga
     1801 tgatttgtct gggccattaa tgaaatagct gccaatggaa ctttcatttg gtgcgaccac
     1861 atctttatca taagtgcaac atgattggtt tgttcaattg tttcagaaac tttttgcgct
     1921 tctctagctt ctaattgtgt aataatatta aaataagcgt aatcactcgg ttgaagttcg
     1981 aaagaggggt cctgactact attaaaatat ttgagatgta acattttttt cctgtaacga
     2041 ctgtattgcc aaatactaat tcctagtaaa acaataacat ttaaacctaa ggaattaaac
     2101 aaatagggca ttggtaaatg atatagatag aaactaataa aaaataggac aaacatcatg
     2161 atatataaaa tataccaaat caagtgttct cttaaaaact ggcgtatcat tgtagtaaat
     2221 acccaactcc tctcactgta tgaatgtaat caaaacctat tgggacaatt tttttacgta
     2281 agcgtgtcat attaacatta agtgtatttt gatcaataaa actatcattt tcccaaagtt
     2341 tctctaatag agactctttt gagactactt gtttaggatg catgagcaag atagatagga
     2401 ttttattttc tgttggcgat aaaataacct ctttatcttg gctagacaat aacccttctc
     2461 ttgtcaacgt aaatccccca aaagttaatt cctgttggat aaattgttga cttctcctta
     2521 aaatagcagt tagcttagca tctaatacag ctagagagaa tggttttgaa ataaagtcat
     2581 caccccccat atttaatgcc ataaccatat ccatttcatc attactagat gaaatgaaaa
     2641 taataggaat tgttaaaaac ttacgcaatt ctgcagtcca gtaaaaacca ttaaaatagg
     2701 gtaacgtaat atccattagt atcaaatcgg gttgaaatgc gataatttct tgtttcacat
     2761 cacgaaaatt gctgacacta gagacatgat agctagctga taaatgatct tttaaaagtg
     2821 acacaatcgt catatcatct tctacaatat aaatttttcc ttgctcttgt gacatatccc
     2881 ctcctaaaat agtacgaaat ctctttcttt catttagtat aacaaatttt ttagtctatc
     2941 ggtttctatt aatgcaaaag gattggtaaa ataaccaatc ctttttagcg ttcaataata
     3001 tgataataag ttcgactagt tatacggtaa atgataaagt aaattataga aatcgcaagt
     3061 actgtcaaac cacttactac atatacgatt gttgagttaa gcaccccaaa taccaaaagc
     3121 atctgtttta gcattggaat agcaactcca aagtgaataa ctgctaccac taaaggttgg
     3181 aagaagaaaa tcataatttg agaattaata gtccgtttaa ctaatttttt gctcatacct
     3241 acttcttgta agatacgata agatctctta tcttcatgac cttctgagta ttgcttatag
     3301 taaacaatta gagctatacc tagcaaaaag cttataccta acagaaatcc agtaaataat
     3361 aacccaccaa acatttgata accttctttt agaaaactct gtttagtgct aatatttcca
     3421 acatacttac catctacctc tattatgtcg tttttagaaa ttgaatttat ctcttgacta
     3481 ttcaaatcca aatatgcctt aaaggttttt ggaaaagtat tgatgttctt agtataaggt
     3541 aaatatgctt ttctaatatt gtcaatttgt ttgttgtttg caaatataat caaacccggg
     3601 ttgtaagtat ttgtagtctc tgggacataa gcctctttta aattttttac aacgtcaaat
     3661 ttatttccca atagatttat ttttttcaaa cggctatcac ctttttgaac aaagtaagcc
     3721 acttggttat cttttaattt tggtaattgg tgacctaaac gacggaaatc attctgagta
     3781 attaggtaca taaaacccgt ttttgtgata tcaacatgtt tgacgttttt agatgtgata
     3841 ttaagttcac ttgattgact aactggcatc gaaatcattg tctgattata agtgatagct
     3901 tccttagatg acttacctag tttcttcaaa atcttttctt caaatatatt tttcgcgtca
     3961 ccttttgaat tatctattga taaacttact gattttggaa atagtccggt aacaacattt
     4021 tgtgtatttg aataaagtga gactgttgta gcaatagtaa ctagagccat aacagctaat
     4081 aaagtgatac ttgctaaccc tactgcattt tgcttcattc gaaaaatcat ttgcgaagtt
     4141 gatacaaaat gctcagattt ataataataa tgcttgtttt gacgcaacct ttttaagtac
     4201 catgtcataa aactaatata aaaaagataa gtaccagcaa taactaaaag tactgcaaag
     4261 aagaaacgat agataacagc taatgcaggt gctttacctg aagtaagagc cattgtataa
     4321 gcgatggcga tagctactaa cgctaaaatt gcaagtataa gattaccttt tggttctttt
     4381 tctccctgtt gtttctttct aaaaagactt aatggggatg ataaatgaac atgtcgaata
     4441 actggaactt ctaaagtcag aaaaatacct gtaaatataa cgatacaaat aataaatggc
     4501 cataaactta aactaagatt tagtgcatga tagttaataa tgttgacaaa aattaaataa
     4561 ataaatttag caaaaaaagc actaaacaga cttcctatag aaataagaaa tatataaata
     4621 ataaacagct ctagactagc tacacgcgca acttgacgtt tattcatccc caaaatgtta
     4681 tacagtccaa attcactact acgctgtttc atcaagatat tgtagctata atgttccatg
     4741 actaccgcaa agattgacaa aacaatcact ccaagaaaaa gaaccgttgc cgctgtcccc
     4801 atatctcttc caacagcact cattaagatt agtagcgtag aacaagtcaa tgaataaagt
     4861 aataaactgg ctaagaggaa tggtatgtac tggtctatag aatgttttaa attatgccag
     4921 gctattttga gataaaacat attattcacc ccctaatatg gcagacattg ttaaagaaat
     4981 atctttattg aattcgctat tattcttatt accacgataa agttggtgaa aaatacgtcc
     5041 atctttgata aataaaacgc gtttggcatg actagcagca tttgctgaat gggttaccat
     5101 caaaatagtt tgtccatcca agttaatagt ttcaaataaa ttcagcaagt cttctgaatt
     5161 acggtaatct aacgctgctg ttggttcatc tgctaataaa atttgagggt tggttatcaa
     5221 actacgagca atcgctacac gttgcttttg cccaccagag agctcgaaag gtctcttatc
     5281 taataagtca tcaattctca gatgagaaga taattctgac aaacgatgat ccatttcttt
     5341 gtaacgttta cgatcaagaa ctaaaggaag atagatatta tctcttacag agagagtatc
     5401 caaaaggttg aagtcctgaa agacgaaacc aagatttttc aaacgaaaac tcgctaattt
     5461 tgcctcttta attttcgtaa tatcttcccc atttaaaatc acttgtccat ttgtcggttt
     5521 ttctaaagta gctaaaatat ttagcagagt tgtttttccc gaaccagact ctcccataat
     5581 agcaatgaat tccccttgtt caactttaaa atcgacgtct tgtagagccc ttgtctcttc
     5641 ttttgaaaaa cgtgtacgaa aaactttttc taagtgattg atttctaata acatataatt
     5701 ctcctttatt tattataccc taattttgat tgattctact ctttaaccaa gactgtgact
     5761 cttgtaaagg aaggctggtt acttgatctg gtaagatagg ggtgttctcg tatttatatc
     5821 cttgtcgatc actaactatt cctgtagtta aagctaatct agcaccgtcg taaagcatga
     5881 aagtttcatt aacagtcgta tatcctgctg tagcttgacc aaagctttta acattaggta
     5941 atcctttaaa tgataaaaaa gtcatttctg ccgaactagc agttttatga ttagttataa
     6001 tagcgattgg aacatgctta gtattgattg ttttcctaga tatttttaat gcttctagcg
     6061 gtatattttt catcgtgatt gttttcttat ttccatactt atcagtataa tgaaataggg
     6121 tatcatttgg taaaatagag gctacccccc aatcataggg atcatattgc cgccagtatt
     6181 attcgataag tctaaaatga gtcccttata acttttttcc atcaatatat tttgtaattt
     6241 accactataa tggctaatag atcgtttatc taacttactt atagaaggaa tagtaattaa
     6301 gagaatgtct tcgtttaatc ttcgatattc tggatatcga ctatctaggg tatctcttcg
     6361 tacagtctct ttggataata tttgactatg tttaccgcct ccataccgta ctgcttcctg
     6421 tacaatgttg cgactctctt ggtaagattt tgacgttttc aacttttttt ctgatcgttg
     6481 tcttataatt tgccattgac gaccttgcga atacaaacca cgctgttcaa cacgatctaa
     6541 aataacacgt ccataacgtt cgctactagg aggtaataga taaatattta atgctgaacc
     6601 ataataatga actaccactc ctaaaattcc taaaactata agcattggaa ctacaaaaag
     6661 taaaactatt tttcgccgca tctcttgctc cccctttctc tttaagtcta ttatatggca
     6721 aaatggttta ctataacctt acatcttaag acctatttct tacaaatttg aaagttttaa
     6781 aagctttata aactataatt ttgatataat atcttacata acaaatggaa agtacggtga
     6841 aaacatatgg ccaagacaag taaaaaattg acaaaatctt tagggcctat tggtaaatta
     6901 atttcaataa tccctgacac aactgaactt attggaaaag caattgataa ttctcgtcca
     6961 attattgaaa aagaacttga ccgtcgtcat gaaaaaaaac agacttatac tcagcttgat
     7021 aatgtagtac accttgatat caatgatgcg caagtttact tagagcaacg ccattttaat
     7081 gtatcaagga ttgttgctga accaaattca aagtgggcaa ccctacatcc taatcaagtt
     7141 gtgaatatgt cgcctaaagc tggtagggtc agaatcggtt cattagtgaa gttgtactat
     7201 attacagagg atatccttga agaaagtaag acactacttt tatctaaatc cgaaaaaagt
     7261 aaagcacgac aacaacggtt aacagaagta ttgagtaccc ctagtcaatt actcaaaaaa
     7321 aatcgaaata aataagcatg atcgaattga ccatgcttat ttatttttac caaacaggtt
     7381 gatctaaacc ttgagatgtt ttttcgataa cttttttaac cgcatctgta tggtaagctt
     7441 tcacaacttc tttaatggct ttagcttttg atttgtcttg ccaacctttt tgagcaacta
     7501 aaaggttgta ccattgtttt gcatttttac tttttggttc aataaaaata gctgatttag
     7561 gatttattcc tgcttctgtc acaaaatcat tattaataac tgctgcatct gtggaatcca
     7621 aagaacgtgc ggtctgtgct gcatctactt cttttaaatc taaagattta ggattggaaa
     7681 caacatctga cattgttgct aatgcatcac ctgatacttt caattttagc aagcctgctg
     7741 attgtaacaa gtagagagag cgactttcgt taactgcatc gtttgggata gtaatagttg
     7801 ctttatttgg aatttcagaa actgtttggt atttaccttt accgttctta gtaccagagt
     7861 ataatctaaa ggaagtaaag tatgtctcag caacggaaac aagattggtc ttatttgctt
     7921 tattccaatt gtttaagtaa ttataatgct ggaaagcatt aatatcaatc tcatcacttt
     7981 ctaaagcttt attaggttgt gtataatctg tgaattccgt aaattttagc ttaacgcctt
     8041 ttttcttaac taattcttcg attttatccc aacgagcttt ttcagtttta ctctttacca
     8101 ttgttccaac tctaacgact ttggcgtctt catttttaga ctgaccacag gctcctaaaa
     8161 taagtgctga aattactact gttgtcgtta accctaatag ctttttgatt ttcataattt
     8221 atttctcctt tgatatggtt gtatgattaa tcaactgata ctagtgtaac aaaaagctaa
     8281 tggattaaaa aatacttatt atcaatcaag agatatagct tttaactata tcatttcttt
     8341 tataatttta ctttcgaggg ctccgtaaag cggtccaata ataggcgact tcctcataac
     8401 cttggtgctc atacattttt ctgttttctt cttctgaaat taagataact ttcttaaacc
     8461 ccctacttgc atcaaattgt aggcgcgtac caattccttg tcctctgtag gactcttgga
     8521 cataaaaatc atctatttcc acgtattcac catagtccca agctgttaca tctccaataa
     8581 tttgattatc tttaatagct aggtaaactt tagcattatt atttaaaaga ttaacacgat
     8641 tatcattata catttgctta gcgtaaattt ctccgtaaac tatactttgt ttatatttga
     8701 actttaggta tttttctagg taattttcat taagtttagt aaatgtgatg tcatcaaatt
     8761 ctttttcaaa aagtttaaga tgatttaatg aatttgtaaa tatgatatgt ttctctgatt
     8821 caaagccttg ttgtgataaa gttctctgta attcactgct caattcagca ttttcctcaa
     8881 aaaatacaaa agcaaaatca gactgataat ctttttgttc atctgctaaa tagatcatat
     8941 cctgtaaaaa ctcacttact gtaggcatta ttttgtagct aatatagttt gaaaaataac
     9001 gctctgaaat gattatattc ttaaaatgtt tataccgatt agtttccaaa taagcttttt
     9061 cggatgactc aagtaattct ttatagtgca tatttactcc attcatacat ttttaaaaat
     9121 caatatagaa aatttgacct tgcttgttgg caaagtcaaa tgaatatatc aatagttgag
     9181 aaatttttct aaatctttca aagctcgtgt agcaatagct tcataacgct ctttcttatc
     9241 acgaattcgt ggaccttcta attctttgat gataccaaag ttaacattca ttggttggaa
     9301 atgtttgctg tctgtatgtg taatataatg tggcagtgca ccaatagcag tagtttgtgg
     9361 gaaaactact tcactctccc cattgaagcg acggacagca ttgattcccg ctacaagacc
     9421 acttgctgcc gattctacat aaccttcaac accggtcatt tgtccagcaa agaaaagatt
     9481 tgggttctta cgtgtagcaa aggtttggtt taaaagattt ggtgaatcca tataagaatt
     9541 gcgatgcata acaccatacc ggacaaattc agcattttca agaccaggaa tcatttgaaa
     9601 tacacgtttt tgttctcccc acttaagatg agtttggaaa ccaactatgt tataaagaga
     9661 gcctgcagca ttatcttgac gtaattgaac aacagcatat ggtgttttaa attcaccatc
     9721 tcgcggtcct ttgtagtcct caggatactc caaaccaact ggtttcattg gaccataaag
     9781 catcgtttta ataccacgct tagccatcac ttcgataggc atacaacctt cgaagtattt
     9841 ttctttctca aaagaattta gtggtgcttc ctcagctgta gttaaagctt catgaaaagc
     9901 cataaattct tctttggtca ttggacaatt tagataagct gcttcacctt tgtcatacct
     9961 agacttaagg taaactttat ttatgtcaat cgtattttta tcgacaattg gagcggcggc
    10021 atcgtaaaaa taaaaaccat caccaccatt aagttcatgg attttagctg ccagactatc
    10081 acttgttaat ggtcctgtag cgataactgt aatagcatct ccagggatat ctgtgatttc
    10141 atcacgaatt acttcgatta atggatgttt atggatttcc tcagtaacag cttcagaata
    10201 accttctcta tcaacggcca tggctccacc tgctggaact cgatgtgcct ccccatttct
    10261 cataatgata gaatccaaac gacgcatttc ttcttttaaa agaccaactg catttgttag
    10321 agaatcaccg cggaatgaat tggaacatac taattctgca aaattatctg ttttatgttg
    10381 aggggttgat ttaaccccac gcatttcata aagcttaact ggtattccac gtttagcaat
    10441 ttgataagct gcttcagaac cagccaaacc agcgccgata acattaatgt aagattgaga
    10501 catgaactaa tacctcttga aagtagtcaa ctacttcctc aaatctttct atataattta
    10561 tttttccatt ataacataca tagctacttc ttcaaaaaac gttattcatt aaactactag
    10621 ttattctcta aataaaactc aaaacgatct ccaacatatt gcccatgaac atattcaaaa
    10681 ggcttaccat cagtaaagta tgaaacttgt gtcaatgcca aaatagcatg gcctgcgtta
    10741 acttctaaat gcgaagcaac acgttcacta gcatttctag catatatagt ttgttggctt
    10801 ttcccaattt cgtagccatt ctctgttaag gttttaaaga aatgttctgt aacctctgat
    10861 tgttcaaaac ctttaattaa attctctggt atagaggcta cctcataaac caacggtacc
    10921 ttatcagcat aacgtaccct ttccatacga accacataat ctgactgtga taaatttaat
    10981 ttttgaattt cggtttcatt tgctagtttt ctttgaaatg agattagttt actggatggc
    11041 ttacgccctt gagaattgac aatttctgta aaactagttg ttccccgcat tttttcttga
    11101 acacgatgac tagcaacata ggtgccacta ccaactcgtc tctccaaaat accttcttca
    11161 actaacaatg taattgattg gcgaagtgtc atacgactta cactataatc atccgctaaa
    11221 tctctttcgc taggtaaccg ctgcccaatc ttccaagtcc ctttatcgat ttccttttta
    11281 atagcgtcgt gaattttaat atatgctggt aacaatattc tctcctccta gaatctacac
    11341 ttcattttac catacttcta tatatttcta actctaaaat cgtgaaataa ttcagaaaaa
    11401 tcataaacaa tatttcttga taagaaagcg tttaatcaat tattttaatg atgttaattt
    11461 atataaaata tgctaaaatg gtataggtac agctgttttt aaaattagct acaaagaaag
    11521 ggaaacaatg actgatattt ctatattgaa tgatatccaa aaaattatcg ttctagatta
    11581 cggtagtcaa tataaccagc ttatcgcacg tcgtatccgt gaatttggtg tattttctga
    11641 gttaaaatca cataaaatta cggctgatga aattcgcgat attaatccta ttggtatcgt
    11701 actttcaggt ggtccaaatt ccgtatacgc tgatggcgct tttggaatcg atgaagaaat
    11761 ttttgaatta ggaattccaa tcttaggaat ttgttatggc atgcaactga ttacccataa
    11821 actcggtggt aaggttcttc ctgcgggaga agctggtcat cgtgagtatg gtcaatcagc
    11881 acttcgccta cgctcagaat cagcactctt tgctggcact cctcaagaac aacttgtttt
    11941 gatgagccat ggtgatgctg tcactgaaat tcctgagggt ttccatctcg ttggtgactc
    12001 tgttgactgt ccatttgctg caatggaaaa tactgaaaaa caattttatg gtatccaatt
    12061 ccaccccgaa gttcgtcact cagtatatgg caatgatatc cttaaaaatt ttgctgttaa
    12121 tatctgtggc gcacgtggtg attggtcaat ggataacttc attgatatgg aaattgcaaa
    12181 aatacgtgaa actgtaggcg atcgtaaagt acttcttgga ctatctggcg gtgttgattc
    12241 atctgttgtc ggagtactcc tacaacgtgc tatcggtgat caattaacat gtatttttgt
    12301 agatcatggt ttattacgta aaaatgaagg tgaccaagtt atggatatgc ttggtggtaa
    12361 atttggactt aatattatcc gtgttgatgc ttcaaaacgc ttcctagatc ttctttctgg
    12421 tgttgaagat cccgagagaa aacgaaaaat catcggaaat gagttcgtgt acgtttttga
    12481 tgatgaggca agtaaactta aaggcgttga tttccttgct caaggtaccc tctacacaga
    12541 tattattgaa tctgggacag aaacagccca aacaatcaag tcacaccata atgttggtgg
    12601 attgccagaa gatatgcaat ttgaattgat tgagccactt aacacattat tcaaagatga
    12661 ggttcgcgct cttggtactg cacttggaat gcctgatgaa gttgtatggc gtcaaccatt
    12721 ccctgggcct ggtcttgcta ttcgcgttat gggtgaaatt accgaagaaa aacttgaaac
    12781 agtccgcgaa tcagatgcta ttcttcgtga agaaatcgct aaagctggtc tagatcgtga
    12841 cgtatggcag tatttcacag ttaatactgg cgtacgttct gtcggtgtta tgggagatgg
    12901 tcgtacttat gactatacaa ttgctatccg tgcaattact tcaattgatg gcatgacagc
    12961 tgactttgcg caacttcctt gggatgtact taagaaaatt tcgacacgta ttgttaacga
    13021 agttgatcac gttaaccgta ttgtttatga tattacaagt aaaccacctg caactgttga
    13081 gtgggaataa tatagaaagt aaaaataaaa gttttacact gattttataa ggtttttcga
    13141 aactagaatg gtataaattc tatcttttta gataaggtac cccgccaatt tccccgccaa
    13201 aaaaattggc atataattac aaaaactctc ttagttttcc actaggagag tttttgtaat
    13261 t
//
LOCUS       DATRNK010000055        13126 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.055, whole genome shotgun sequence.
ACCESSION   DATRNK010000055 DATRNK010000000
VERSION     DATRNK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13126
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.055"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            263..652
                     /locus_tag="VBC52_001574"
     CDS             263..652
                     /locus_tag="VBC52_001574"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEO6883148.1"
                     /translation="MGNIELKKLAVKASENAYVPYSKFPVGAALRTAEGKIFTGCNVE
                     NISYGLANCAERTAIFKAVSEGYKDFSEIAIYGNTEEPISPCGACRQVMVEFFNKNAK
                     VTLIAKNGKTVETTVGELLPYSFVDLK"
     gene            717..1766
                     /locus_tag="VBC52_001575"
     CDS             717..1766
                     /locus_tag="VBC52_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO6883149.1"
                     /translation="MNKKIAGIGLASIAVLSLAACGHRGASKSGGKSDSLKVAMVTDT
                     GGVDDKSFNQSGWEGMQAWGKKNGLKKGAGFDYFQSASESDYATNLDTAVSSGYKLIF
                     GIGFSLHDAIDKAADNNKDVNYVIVDDVIKGKDNVASVVFADNESAYLAGIAAAKTTK
                     TKTVGFVGGMESEVITRFEKGFEAGVKSVDKSIKIKVDYAGSFGDAAKGKTIAAAQYA
                     SGADIVYQVAGGTGAGVFSEAKSRNESLKEADKVWVLGVDRDQAAEGKYTSKDGKASN
                     FVLASSIKEVGKSVELIATKTSKGKFPGGNVTTYGLKDGGVDIATTNLSDDAVKAIKE
                     AKAKIISGDIKVPSK"
     gene            1911..3446
                     /locus_tag="VBC52_001576"
     CDS             1911..3446
                     /locus_tag="VBC52_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6883150.1"
                     /translation="MTQNVIEMKEITKKFGDFVANDHINLTVEKGEIHALLGENGAGK
                     STLMNMLAGLLEPTDGQIFINGQPVTIDSPSKSSQLGIGMVHQHFMLVEAFTVAENIV
                     LGNETTQNGVLDIKTAAKEIKELSEKYGLSVNPNAKISDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPSEIKELMTIMKSLVKEGKSIILITHKLDEIRAVADKVTVIRRGK
                     SIETVPVAGASSQQLAEMMVGRSVSFRTEKKEANPTDIILSVKDLVVEENRGGVLAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVTSGQIVIKGKDVTKFSSRQITEL
                     SVGHVPEDRHRDGLVLDMTMAENLALQTYYKEPLSHKGILNFAKIKEYARQLMTEFDV
                     RGAGEHVLARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DKGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVKPDQTNKQELGILMAGGKIEKEE
                     RDV"
     gene            3439..4500
                     /locus_tag="VBC52_001577"
     CDS             3439..4500
                     /locus_tag="VBC52_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6883151.1"
                     /translation="MSKKAQKIAVPLISVVLGIILGAIIMLIFGYDPLWGYEGLFQTA
                     FGSIKNIGEIFRAMGPLILIALGFSVASRAGFFNIGLPGQALSGWIAAGWFALSHPDM
                     PRPAMILCTIIIGIVAGGITGAIPGILRAYLGTSEVIVTIMMNYIVLYSGNAIVQRVF
                     PKSIMRTSDSSVYVSANASYQTDWLSSLTNNSRINIGIFIAIIAVVLVWFLLNKTTLG
                     FEIRSVGLNPNASEYAGMSAKRTIILSMIISGAFAGLGGVVEGLGTFENVFVQPSSLA
                     IGFDGMAVSLLAANSPIGILFAAFLFGVLSVGAPGMNIAGIPPELIKVVTASIIFFVG
                     VHYIIEYVIKPKKQMKGGK"
     gene            4502..5458
                     /locus_tag="VBC52_001578"
     CDS             4502..5458
                     /locus_tag="VBC52_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6883152.1"
                     /translation="MSLTTIFALLFSSMLIYATPLIFTSIGGTFSERGGIVNVGLEGI
                     MVIGAFSGVVFNLEFASVFGDATPWISVLVGGLVGLIFSVIHAVATVNFRADHIISGT
                     VLNLMAPSLAVFLIKVLYNKGQTDNIQESFGKFNFPILSDIPFVGDIFFKGTSLVGYI
                     AILFSFLAWFILYKTRFGLRLRSVGEHPQAADTLGINVYLMRYSGVLISGFLGGIGGA
                     VYAQSISVNFAATTILGPGFISLAAMIFGKWNPIGAMLASLFFGLSQSLAVIGSHLPL
                     LSNIPTVYLQIAPYVLTIIVLAAFFGQAVAPKADGINYIKTK"
     gene            5663..7033
                     /locus_tag="VBC52_001579"
     CDS             5663..7033
                     /locus_tag="VBC52_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO6883153.1"
                     /translation="MSKIVVVGTNHAGTAAIKTMLSNYGEANEIVTFDQNSNISFLGC
                     GMALWIGEQIDGPEGLFYSDKEQLESMGAKVYMNSPVLNIDYDKKEVTALVDGKEHVE
                     SYEKLILATGSQPIIPPIKGVEIQEGSREFKATLENLQFVKLYQNSEEVIEKLAKPGI
                     NRVAVVGAGYIGVELAEAFQRIGKEVTLVDVADTCMGGYYDRDFTDMMSKNLEDHGIR
                     LAFGQAVQAVEGDGKVERLVTDKETFDVDMVILAVGFRPNTELGAGKLDTFRNGAWVV
                     DKKQETSVKDVYAIGDCATIWDNSRDDINYIALASNAVRTGIVAAHNACGTELEGAGV
                     QGSNGISIYGLNMVSTGLTLEKAKQAGYNAVETGFNDLQKPEFIKHNNHEVAIKIVYD
                     KDSRVILGCQMVSHEDVSMGIHMFSLAIQEKVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(7154..8143)
                     /locus_tag="VBC52_001580"
     CDS             complement(7154..8143)
                     /locus_tag="VBC52_001580"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO6883154.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPALF
                     DKAVGDAEDLSHALAFTSPKKIYAATYADCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFNGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALADKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVQLEQWLQENRDIDEQGLVDLF
                     ISVRDAAYSIINKKGATYYGIAVALARITKAILDDENAVLPLSVYQEGQYGDVKDVFI
                     GQPAIVGAHGIVRPVNIPLNDAELQKMQASAEQLKDIIDEAWKNPEFQEASKN"
     gene            8382..10841
                     /gene="gyrA"
                     /locus_tag="VBC52_001581"
     CDS             8382..10841
                     /gene="gyrA"
                     /locus_tag="VBC52_001581"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEO6883155.1"
                     /translation="MQDKNLVDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALEMLRDINKNTVDFQDNYDGSEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGESIDAVKLVMDNPDVTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYETGKGSIVLRSRTEIETTSNGKERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRIEGITAVRDESSREGVRFVIEVRRAASANVILNNLFKLTSLQTNFSFNMLAI
                     EKGVPKILSLRQIIDNYIEHQKEVIVRRTQFDKAKAGARAHILEGLLVALDHLDEVIT
                     IIRNSETDTIAQAELMSRFELSERQSQAILDMRLRRLTGLERDKIQSEYNDLLALIAD
                     LADILAKPERVVTIIKEEMDEVKRKYADARRTELMIGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGIQGTGVNNDDFVRELVSTSTHDTVLFFTNLGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLDEGETIQTIINARKEDVANKYFFFTTQQGIVKRTS
                     VSEFSNIRQNGLRAINLKENDELINVLLIDENEDVIIGTRTGYSVRFKVNAVRNMGRT
                     ATGVRGVNLREGDKVVGASRIVNGQEVLIITEKGYGKRTEASEYPTKGRGGKGIKTAN
                     ITAKNGPLARLVTINGNEDIMVITDTGVIIRTNVANISQTGRSTMGVKVMRLDQEAKI
                     VTVALVEQEIEDKSNIEDTKE"
     gene            10848..11591
                     /locus_tag="VBC52_001582"
     CDS             10848..11591
                     /locus_tag="VBC52_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEO6883156.1"
                     /translation="MRNKKKSHGFFNFVRWLLVVLLIIVGLALVFNKPIRNAFIAHQS
                     NHYQISRVSKKTIEKNKKSKTSYDFSSVKSISTESILSAQTKSHNLPVIGGIAIPDVE
                     INLPIFKGLGNTELSYGAGTMKENQIMGGPNNYALASHHVFGLTGSSKMLFSPLEHAK
                     KGMKVYLTDKSKVYTYTITEISKVTPEHVEVIDDTPGKSQLTLVTCTDPEATERIIVH
                     AELEKTGEFSTADESILKAFSKKYNQINL"
     gene            11607..12020
                     /locus_tag="VBC52_001583"
     CDS             11607..12020
                     /locus_tag="VBC52_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6883157.1"
                     /translation="MKLKTVHHIAIIVSDYEKSKDFYVNKLGFEIIRENHRPERHDYK
                     LDLRCGDIELEIFGNRLDDPEYETPPQRIGRPNWPREACGLRHLAFYVPDVEAYKVEL
                     ENLGIFVEPIRYDDYTGKKMTFFFDPDGLPLELHE"
     gene            12173..>13126
                     /locus_tag="VBC52_001584"
     CDS             12173..>13126
                     /locus_tag="VBC52_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEO6883158.1"
                     /translation="MSIKKLLFAGLAFILFTLASPAYAASDVQKVIDETYVQPDYVLG
                     YSLNQEQRAQTLQLLNYDESRDTKVKTLNTSSYAKIMNIADDASIQLYSSVKIKKLGS
                     NDTLAVNIVTPENITKVTEDMYRNAAVTLGIEHATISVAAPIKVTGESALAGIYYSLE
                     KNGASVSSENKQLAQEELSTLSGINAENKGKEGYDADKLNVALTDIKSAVAKGGSDLS
                     KDDIRKIVEETLKNYHLDNAVTENQINLIVNFAVNLSQSNVIKNSDFTNTLNNLKDNI
                     VSKAGSKFKNINVNFNANKAVESGKGFLANIWQQIVNFFQQF"
BASE COUNT         4253 a         2016 c         2709 g         4148 t
ORIGIN      
        1 gaaaggtttt ctctaaaaat gttttcttta aattccgtat aagataaaaa gaattctgct
       61 ttttattctg aaaattataa aaaactactt acattatcta aaaaaaatgg taaaatagat
      121 tcagctaaaa taccaagact aatgaaacta caagacttgg ttactcaata ataaatactt
      181 ctgtgtttga taagaagata ataaacgtgt tctaaaaatt ttgccagtaa acattatgaa
      241 gcgaaaggtg cagctactaa aaatgggaaa tatagagttg aaaaaattgg ctgtaaaagc
      301 gagtgaaaat gcatacgttc cttactcgaa atttccagta ggcgcagcat tgcgtacagc
      361 agaaggaaaa atatttacag gatgtaacgt tgaaaatatc agttatggat tagcaaactg
      421 tgctgagcgt actgcaattt ttaaagctgt ttcagaaggt tataaagatt tttcggaaat
      481 tgctatttat ggaaatactg aagaaccaat ttctccttgc ggtgcatgcc gtcaagtgat
      541 ggtggaattt tttaataaga atgctaaagt tacattgatt gctaaaaatg gtaagacggt
      601 cgagacgacg gtcggtgagt tgttaccata ctcgtttgtt gatttaaaat aatttaaatc
      661 agttgctctt ttaagcattt agcttaaata ttaagttttt taggagggtt catttcatga
      721 acaaaaaaat tgccgggatc ggcttggctt cgattgcagt acttagttta gctgcatgtg
      781 gacatcgtgg tgcttctaaa tctggtggta aatcagatag cttgaaggtt gcaatggtaa
      841 cagataccgg tggtgttgat gataaatcat ttaaccaatc tggttgggaa ggtatgcaag
      901 cttggggcaa gaagaatggc cttaaaaaag gagctggttt tgactatttc caatcggcaa
      961 gtgaatctga ttatgcaact aacttagata cagctgtgtc tagtggttat aaattgattt
     1021 tcggtattgg attttctctt catgatgcta ttgataaagc agcagacaat aacaaagatg
     1081 ttaattacgt catcgttgat gatgttatta aagggaaaga taatgttgca agtgttgtct
     1141 ttgcggataa tgaatcagct tacttagcag gtattgcagc cgctaaaact accaaaacaa
     1201 aaacagttgg ctttgtaggt ggtatggaat ctgaggttat tacccgtttt gaaaaaggtt
     1261 ttgaagcagg tgtcaaatca gttgataaat caattaaaat taaagttgac tatgctggtt
     1321 cattcggtga tgctgctaag ggtaagacaa ttgcagccgc acaatatgct tctggcgcag
     1381 atattgttta tcaagtagct ggtggaactg gtgcaggtgt attcagtgaa gcaaaatcac
     1441 gcaatgaatc tttaaaagaa gctgacaaag tttgggtatt aggtgtagac cgtgaccaag
     1501 cagcagaagg taagtatact tctaaagatg gtaaggcttc taactttgtt ttggcttcat
     1561 caattaaaga agttggaaaa tcagttgaat tgattgctac taaaacaagt aaaggtaaat
     1621 tcccaggtgg taatgtaaca acttatggtt tgaaggatgg tggagttgat atagcaacta
     1681 caaacctttc agatgacgcc gttaaagcga ttaaagaggc taaagctaaa attatttcag
     1741 gagatatcaa agttcctagc aaataatatc tttaagaact ttcaaaaaag cgattagaaa
     1801 tcgctttttt gaagctgaga taaacttata tctcagtaaa atccatcagt cgttaacgat
     1861 tggtggattt tactgaacta taaatgaatt tttctgaaag ggagaaccac atgacacaga
     1921 atgtcattga aatgaaagaa atcactaaga aattcggtga ttttgtggca aatgatcata
     1981 tcaatttgac ggttgaaaaa ggtgagatac atgctctgct tggcgaaaac ggtgcaggga
     2041 aatcaacttt aatgaacatg ttagcaggtt tactagaacc aacagacggt caaattttta
     2101 ttaatggtca gcctgtaact attgattcac cttccaaatc gtcacaattg gggattggta
     2161 tggtacatca gcatttcatg cttgttgaag catttactgt agctgaaaat attgttctag
     2221 gaaatgaaac cacccaaaat ggtgttttag acattaagac agcagctaaa gaaataaagg
     2281 aactatcaga gaaatatggt ttgtcagtaa atccaaatgc taaaatatct gatatttctg
     2341 taggagcaca gcagcgtgtt gaaattttga agacacttta tcgcggtgca gatattttaa
     2401 tttttgatga gccgacggcg gtgcttacac catcagaaat aaaagaattg atgacaatta
     2461 tgaagagcct tgttaaagaa ggcaaatcca ttatactaat tacccataaa ttggatgaaa
     2521 ttcgggcagt tgctgataaa gttactgtga ttcgccgtgg taaaagtatt gaaacagtcc
     2581 ctgttgcagg cgcaagttct caacaactgg cggaaatgat ggttggacga tctgtttcct
     2641 tcagaactga aaaaaaggaa gctaatccga ctgacataat cctttctgta aaggatttag
     2701 ttgtagaaga aaatagaggc ggcgttctag ctgttaaaaa tctgtcgtta gatgttcgag
     2761 caggtgaaat tgtaggtata gctggtattg atggtaatgg acaaagtgaa ttaattcaag
     2821 ctattacagg tttaagaaag gtaacttctg gtcaaattgt aattaaaggt aaagatgtca
     2881 caaaattttc aagtcgacaa attacagaat taagtgtagg tcatgttcca gaagatcgtc
     2941 atagagatgg tttggttcta gatatgacga tggcagaaaa tttagcttta caaacttatt
     3001 ataaagagcc attgagtcat aaaggtatcc ttaattttgc taaaattaaa gagtatgctc
     3061 gtcaattaat gacagaattt gatgtgcgtg gtgctggtga acatgtttta gcaagaggat
     3121 tttctggtgg taaccaacaa aaagcaatta ttgctcgtga agtggatcgc gatccagacc
     3181 tactcattgt tagccaaccg actcgtggtc tagatgttgg tgctatcgag tatattcata
     3241 aacgtttgat tgaagagcgc gataaaggga aagcggtctt agtagttagc tttgagttag
     3301 atgaaatctt aaacttatca gatcgtattg cagttattca tgatggtaaa attcaaggaa
     3361 ttgttaaacc cgaccaaacc aacaagcaag aattgggtat tttgatggct ggtggaaaaa
     3421 ttgaaaagga ggagagagat gtctaagaag gcgcaaaaaa ttgcagttcc tctgatttca
     3481 gttgtactag gaatcattct aggagctatt attatgctga tatttggtta tgatccactt
     3541 tggggttatg agggattgtt ccaaacagca ttcggtagta taaaaaatat tggtgaaatt
     3601 ttccgtgcaa tgggaccatt aattttaatt gctctaggtt tctcagttgc cagtcgtgca
     3661 ggtttcttta atattggact tccagggcaa gccttatcag gatggattgc cgcaggttgg
     3721 ttcgctcttt cacacccaga tatgccacgt ccagcaatga ttctttgtac tattattatt
     3781 ggtatcgttg ctgggggaat cactggagct attccaggta ttctcagagc ataccttgga
     3841 acaagtgaag ttattgtaac tattatgatg aactatattg ttctttattc aggaaatgct
     3901 attgtacaac gtgttttccc aaaaagcatt atgcgtacaa gtgactcgag tgtatatgtt
     3961 tctgccaatg catcatacca gacagattgg ttatctagtt taacgaataa ttcacgtatc
     4021 aatattggta tcttcattgc tattattgca gttgtgttag tttggttcct attaaataaa
     4081 acaacgcttg gatttgaaat tcgttcagtt ggtttaaatc caaatgctag tgagtacgct
     4141 ggtatgtctg caaagcgtac aattattcta tcaatgatta tttctggagc ttttgcagga
     4201 cttggtggtg ttgttgaagg actaggtaca tttgagaatg tatttgtaca gccatcctcg
     4261 ttagctattg gctttgacgg tatggcagta agtctactcg cagcgaatag tccaataggt
     4321 attttatttg cagcatttct atttggtgtt ctaagtgttg gtgcgcctgg tatgaacatt
     4381 gcaggtattc cacctgagtt aattaaagtt gtaacagctt caatcatctt cttcgtaggt
     4441 gtccattaca ttattgagta tgttattaaa cctaaaaaac aaatgaaagg tggtaagtaa
     4501 attgagttta acaacgattt ttgcattgct attttcatca atgctaattt acgcaacacc
     4561 tcttatcttt acaagtattg ggggaacctt ctctgaacgt ggtggtatcg tcaacgttgg
     4621 tttagaagga attatggtaa ttggagcttt ctcaggcgtt gtatttaatt tagagtttgc
     4681 ttctgttttt ggtgatgcga ctccatggat ttctgtacta gttggtggtt tagttggttt
     4741 aattttttct gttatccatg cagttgcgac agttaatttt cgtgcagatc atattatcag
     4801 cggaaccgtt cttaatttaa tggcaccttc attagctgtt ttcttgatta aggttcttta
     4861 taataaaggt caaactgata atattcaaga atcatttgga aaatttaatt tccctatact
     4921 atcagatatt ccttttgttg gtgatatctt ctttaaagga acaagcttag tcggttatat
     4981 tgctattctt ttctcattcc ttgcttggtt tattctatat aaaactcgct ttggattaag
     5041 attacgctca gttggagaac atcctcaagc agcagacacc ttaggtatta atgtttatct
     5101 tatgcgttac tctggtgtct tgatttcagg tttccttggt ggaataggag gagctgtata
     5161 tgctcaatct atttctgtta actttgcagc gacaactata ttaggaccgg gctttatctc
     5221 cttagcggcg atgatttttg gtaagtggaa tccaattggt gcaatgcttg ctagtctatt
     5281 ctttggttta tcgcaaagtt tagcagttat cggtagccat ttaccacttt tgagcaatat
     5341 tccaacagtt tatctacaaa ttgctccata tgtcctcact atcattgtat tagcagcttt
     5401 ctttggacaa gcagtagctc ctaaggcgga tggtattaat tatattaaaa cgaaataaat
     5461 aaaaagaaac tagtataagc tagtttcttt ttattgtttg tgaatttgtg gaaaaatact
     5521 ttgtgaaaat ttcacataaa ttataataca ataacaataa aatataaaaa aatagaaaat
     5581 tgagtcatta ttctagtaag agtttgtgaa taatgttata atgtgttttg ttattaatta
     5641 tttataaagg agaactctta atatgagtaa aatcgttgtc gtagggacaa accacgctgg
     5701 tacagcagca attaaaacaa tgcttagtaa ttatggtgaa gccaatgaaa ttgtaacatt
     5761 tgatcaaaat tccaatatct ctttcttagg atgtggtatg gctctttgga ttggtgaaca
     5821 gattgatggt ccagaaggat tgttctactc tgataaagag cagttggaaa gtatgggagc
     5881 taaggtttac atgaactctc cagtacttaa tattgactac gataaaaaag aagttacagc
     5941 tttagtagat ggaaaagaac atgttgaaag ttatgaaaaa cttattttag caactggttc
     6001 acaacctatc attccaccaa ttaaaggtgt tgaaattcaa gaaggatctc gtgaatttaa
     6061 agctactctt gaaaaccttc aatttgtgaa actttatcaa aattcagaag aagttattga
     6121 aaagttagct aaacctggta tcaatcgtgt tgctgttgtt ggggctgggt atattggtgt
     6181 agaattagca gaagctttcc aacgtattgg aaaagaagta acacttgttg atgttgctga
     6241 tacttgtatg ggtggttact atgatcgtga ctttactgat atgatgagta aaaaccttga
     6301 agaccatggt attcgtcttg ctttcggaca agctgttcaa gcagttgaag gtgatggtaa
     6361 agtagaacgt cttgttactg ataaagaaac atttgatgtt gatatggtga tcttagctgt
     6421 tggattccgt ccaaatacag aactcggtgc aggtaaatta gatacattcc gtaatggcgc
     6481 ttgggtagtt gataagaagc aagaaacaag cgtgaaagat gtttatgcta taggtgattg
     6541 tgcgacaatt tgggataatt ctcgtgatga tatcaattat attgctcttg cttcaaacgc
     6601 cgttcgtact ggtattgtag cagctcacaa tgcatgtggt acagagcttg aaggtgctgg
     6661 tgtccaaggg tcaaatggta tctcaatcta tggccttaac atggtgtcaa ctggtttgac
     6721 tttagaaaaa gcaaaacaag ctgggtacaa tgcagttgaa acaggcttta atgaccttca
     6781 aaaaccagaa tttatcaaac acaataatca tgaagtagct attaagattg tctatgataa
     6841 agatagccga gttatccttg gttgccaaat ggtctcacac gaagatgtat ctatgggaat
     6901 tcatatgttc tcacttgcta ttcaagaaaa agttactatt gaaaagcttg ctttgaccga
     6961 tatcttcttc ttaccacatt ttaataagcc atataactat attacaatgg ctgcattagg
     7021 cgctaaagat taatttaaaa ttaaaaaggt taagcgaaat gcttagcctt ttatttcatc
     7081 ctttaacaag gtcaaaaatg taacggcaaa gaaaaagcta agatattcct atcctagctt
     7141 tttttcaact atgttagttt tttgatgctt cttggaattc tgggtttttc caagcttcgt
     7201 caataatatc ttttaattgt tcagctgagg cttgcatttt ttgaagttca gcatcattta
     7261 atgggatatt aactggacga acgataccat gtgcacctac gattgcaggc tgaccgataa
     7321 agacatcttt aacatcaccg tattggcctt cttgatatac agataatgga agaactgcat
     7381 tttcatcatc aaggatagct ttagtaatac gagcaagtgc gacagcaata ccgtaatatg
     7441 tagcaccttt tttgttgatg attgagtatg cagcgtcacg aactgaaata aacaaatcaa
     7501 caagtccttg ttcatcaata tcgcgatttt cttgtaacca ttgttcaagt tggacaccag
     7561 caacgttagc atgtgaccaa acggcaaatt ctgagtcacc gtgttcaccc atgatgtatg
     7621 cgtgaactga acgagcatca acaccaattt tatctgctaa agcttgacgg aaacgtgcag
     7681 agtcaagtga agtacctgaa ccaataacgc gttccttagg gaaacctgag aatttccaag
     7741 ttgaatatgt aagtacatct acagggttag ccgctactaa gaagataccg ttgaaacctg
     7801 attcaacaac ttgagttacg attgatttgt taattgctaa gtttttacca acaaggtcaa
     7861 gtcgagtttc acctggtttt tgaggtgcgc cagcagtaat gacaacaagg tcagcatctg
     7921 cgcagtctgc ataagtagct gcgtagattt ttttaggtga tgtaaatgca agggcatgtg
     7981 aaagatcttc agcatcacca acagctttat caaataaagc tgggatttca atgataccta
     8041 actcttgcgc aataccttgg ttaacaagtg caaaagcgta agaagaacct acggcaccat
     8101 caccaacgag aataactttt ttatgttgtt tagttgcagt catttctaaa catctcctta
     8161 aatatttttg ggaagtttcc catacaccta tattctatca ttttcaatga aagaagtcac
     8221 taataaaatg tgaaaaaatt tgcaaacaaa ttgaaatatc atagaaaact attgaaaacg
     8281 ctgagtgtct tgatattttt gataccgatt tatcatcgca attttaccta aattatggta
     8341 caatgtaaga gacttacagt gaagaaaagg ggcatttttt aatgcaagat aaaaatttag
     8401 tagatgttaa tctaactagt gaaatgaaaa cgagttttat cgattacgcc atgagtgtca
     8461 ttgttgctcg tgcacttcca gatgttagag atggtttaaa acctgttcat cgtcgtattt
     8521 tatatggtat gaatgaacta ggtgtgacac ctgataaacc tcataagaaa tcagcacgta
     8581 ttactggtga tgttatgggt aaataccatc cacatggtga ttcatctatt tacgaagcaa
     8641 tggtgcgtat ggcacaatgg tggtcatatc gtcatatgct cgttgatggt catggaaact
     8701 ttggttcaat ggatggggat ggtgctgccg cacagcgtta tacagaagca cgtatgagta
     8761 aaattgctct tgagatgctt cgtgatatca ataaaaatac agttgatttt caagataact
     8821 atgatggcag tgaacgtgaa cctcttgttt taccagcgcg tttccctaat ttattagtca
     8881 atggtgcaac tggtattgct gtaggtatgg caacaaatat tccaccacat aatctaggtg
     8941 agtctataga tgctgtcaaa ttggttatgg ataaccctga tgttactaca cgggagttaa
     9001 tggaagttat tcctggtcca gattttccta ctggtgcctt ggtgatggga cgttcaggta
     9061 ttcaccgtgc ctatgaaact gggaagggat caattgtctt acgttcacgg actgaaattg
     9121 aaacaacttc aaatgggaaa gagcgtattg ttgtcacaga gtttccatat ggcgttaata
     9181 aaacaaaagt acacgagcat attgtacgtt tagcacaaga aaagcgcatt gaaggtatta
     9241 ctgctgttcg tgatgaatca agtcgtgaag gcgttcgttt tgttattgaa gttcgtcgtg
     9301 ctgcttcagc aaatgtgatt ttaaataatc ttttcaaatt aactagcttg cagacaaatt
     9361 ttagcttcaa tatgcttgct attgaaaaag gtgttccaaa aattttgtca ttacgacaaa
     9421 tcattgataa ctatattgaa caccaaaagg aagttattgt tcgtcgtact cagtttgaca
     9481 aagctaaagc aggagcccgt gctcatatct tagaaggttt acttgtggcg cttgatcact
     9541 tggatgaagt tatcacaatt attcgtaata gtgagacaga caccattgct caagcagagt
     9601 tgatgtcacg ttttgaatta tcggagcgtc agagtcaagc tattttagat atgcgtcttc
     9661 gtcgtttaac tggattagaa cgcgataaaa tccaatcaga atacaatgac ttactagctt
     9721 taattgctga tttagcagat atcttagcta agcctgaacg tgttgttacc attataaaag
     9781 aagagatgga tgaggttaag cgtaagtatg ctgatgcccg tcgtacagaa ttaatgattg
     9841 gtgaagtttt atcacttgaa gatgaagacc ttattgagga agaagatgtt cttattactc
     9901 tctcaaacaa gggatatatc aagcgtctcg ctcaagatga gtttagagca caaaaacgtg
     9961 gaggacgcgg tattcaaggg actggagtta ataacgatga ttttgttcgt gaattggttt
    10021 caacaagtac tcacgatact gtgcttttct ttacaaattt agggcgtgtg tatcgtttga
    10081 aagcatatga aattccagaa tatggacgta cagctaaagg cctccctatc gttaaccttt
    10141 taaaacttga cgaaggtgag acaattcaaa caattattaa cgctagaaaa gaagatgtcg
    10201 ctaataaata tttctttttc acaactcagc aaggtatagt gaaacgtaca agtgtttctg
    10261 aatttagtaa cattcgtcaa aatggacttc gtgctattaa tttgaaagag aatgacgagt
    10321 tgattaacgt cctcttgatt gacgaaaatg aagatgttat tatcgggaca cgtacaggct
    10381 actctgttcg cttcaaagtg aacgctgtac gtaatatggg acgcacagcg actggggtac
    10441 gaggcgttaa tcttcgtgaa ggtgataaag ttgtgggagc ttctcgtatc gttaatggac
    10501 aagaagtact aatcatcact gaaaaaggtt atggcaagag aacagaagct tcggaatatc
    10561 ctacaaaagg ccgtggtggt aaaggaataa aaaccgctaa tattactgct aaaaatgggc
    10621 cattagcacg cttagtgacg attaatggaa atgaggacat catggtcatt acagatacag
    10681 gtgttattat tcgaaccaat gttgctaata tttctcaaac tggtcgttct accatgggtg
    10741 ttaaagtgat gagacttgat caagaagcta aaatcgtcac tgttgcgcta gtggaacaag
    10801 aaattgaaga taagtctaat atagaggata caaaagaata ggaagttatg cgtaataaaa
    10861 aaaaatcaca tggtttcttt aactttgtta gatggttatt ggttgtattg ctaattattg
    10921 ttgggttggc tttggtgttc aataaaccaa ttcgcaatgc tttcatagct catcaatcaa
    10981 atcattatca aatttcacga gttagtaaaa aaacaattga aaaaaataag aaatctaaaa
    11041 caagttatga cttttcatca gtcaaatcta ttagtacaga atcgatttta tctgctcaaa
    11101 cgaaatcaca taatttgcct gtaataggag ggattgctat accagatgtc gaaatcaatc
    11161 tcccaatttt caaaggactg ggcaatacag agttgagtta tggtgcagga acgatgaaag
    11221 aaaaccagat tatgggaggc ccaaataact atgccttagc tagtcaccat gtctttggtt
    11281 tgacaggttc ttctaagatg ctcttttcac ctttagaaca tgctaagaaa ggtatgaaag
    11341 tttacctcac tgataaaagc aaagtataca cttacactat tactgaaatt tcaaaggtaa
    11401 caccagaaca tgttgaggtt attgatgata cacctggcaa gagccaactc actttagtga
    11461 cctgtacgga tccagaagcc acagaacgta ttattgtgca tgctgaatta gaaaaaacag
    11521 gtgaattttc aacagcagat gagtcgatat taaaagcatt ttcgaaaaaa tataatcaaa
    11581 ttaatctcta gaaggtagtt agaattatga aattaaagac tgttcatcat attgcgataa
    11641 ttgtttctga ctatgaaaag tcaaaagact tctatgttaa taagctagga tttgaaatta
    11701 ttcgtgaaaa ccatcgtccg gagcgtcatg attataaatt agatttacgg tgcggtgata
    11761 ttgagcttga aatatttgga aatcgtcttg atgatccaga atatgaaact cctcctcaga
    11821 gaataggtag gccgaattgg ccacgtgaag catgtggttt acgacacctt gccttttacg
    11881 ttccagatgt ggaagcgtac aaggtagaac tggagaattt aggaattttt gtagagccta
    11941 ttcgttacga tgactatact ggcaaaaaga tgaccttttt ctttgatcca gatggtttac
    12001 ctttagaatt acatgaataa taaacaaagt tgcgtggata cgcaactttg tttaatttac
    12061 tgaataattt tatattcaag aagtttttta aagaaataag taagttaatc atggtataat
    12121 gtaaggtatg aaaagaattg aaaaggaatt attttgaaat gaggatacta aaatgagtat
    12181 taaaaagcta ttgtttgcag gccttgcttt tattttattt accctagcga gtccagcata
    12241 tgctgcatca gatgttcaga aagttattga tgaaacttat gttcaacctg attatgtctt
    12301 aggttattcg ctaaatcaag aacagcgtgc acagacatta caacttctga attatgatga
    12361 atctagagac acgaaagtta aaacgctaaa tactagctca tatgctaaaa taatgaatat
    12421 agctgatgat gcaagcatcc aattatattc atctgttaaa ataaaaaaat tagggtctaa
    12481 cgatacctta gcagtaaaca ttgttacacc tgagaatatt actaaagtta cagaagatat
    12541 gtatcgcaat gctgcagtta cattaggtat tgagcatgca acgatatcag tggctgctcc
    12601 aataaaagta actggggaga gtgctcttgc aggtatctat tattcattag agaaaaatgg
    12661 agcaagtgta tcatcagaaa ataaacaact agcacaagaa gagttaagta ctttatctgg
    12721 tattaatgct gagaataagg gtaaggaagg ttatgacgcc gacaagctta atgtagcttt
    12781 aacggatatt aagtcagccg tagctaaagg aggatctgat ctttcaaaag atgatattcg
    12841 aaaaattgtt gaggaaacat tgaaaaatta tcatcttgat aacgctgtaa cggaaaatca
    12901 aataaattta attgttaatt ttgcagttaa tctttcacaa agtaatgtca tcaaaaatag
    12961 tgatttcacg aacaccttga ataatttgaa agacaacatt gtttcgaaag caggatctaa
    13021 atttaaaaat atcaatgtca attttaatgc taataaagct gttgaatcag gtaagggatt
    13081 tttagctaat atttggcaac aaattgttaa ttttttccag cagttt
//
LOCUS       DATRNK010000056        12446 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.056, whole genome shotgun sequence.
ACCESSION   DATRNK010000056 DATRNK010000000
VERSION     DATRNK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12446
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.056"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(118..345)
                     /locus_tag="VBC52_001585"
     CDS             complement(118..345)
                     /locus_tag="VBC52_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO6883159.1"
                     /translation="MPQKNALTGMDQFRNALLSEFSQAKIIDVHPNLITIPLESEFSL
                     PYDLIYSNNPSSSTLGFIKTIADSKLTFSID"
     gene            complement(378..677)
                     /locus_tag="VBC52_001586"
     CDS             complement(378..677)
                     /locus_tag="VBC52_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883160.1"
                     /translation="MSKLAIEKGRSIASQKEIIKIGTADIFKPRLLMKFVNYFKEHIF
                     PYDFAFPHTSEAECDVVEAIYCDIAWKGHCQFYELFQTPLAVAVNSNHPLALKKR"
     gene            complement(688..861)
                     /locus_tag="VBC52_001587"
     CDS             complement(688..861)
                     /locus_tag="VBC52_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6883161.1"
                     /translation="MYIQQLETFVKVAEEGSFSKAAKKLYISTSAVAQQINLLENRYQ
                     FNTCRSVFIHGWS"
     gene            981..1082
                     /locus_tag="VBC52_001588"
     CDS             981..1082
                     /locus_tag="VBC52_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEO6883162.1"
                     /translation="MTKTIVITEASSGIGEATAKFFAKKGWQVAATM"
     gene            1122..1433
                     /locus_tag="VBC52_001589"
     CDS             1122..1433
                     /locus_tag="VBC52_001589"
                     /inference="COORDINATES: protein motif:HMM:NF012334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEO6883163.1"
                     /translation="MKVFKLDVTDSFSIQKAFLEIKEEYKTIDCLLNNAGYALFGPLE
                     LSSEEQIRRAYDTLSLGVTFVTKQFIPLLRENGGGSIITISSVGGVLTLPLTATYHAA
                     K"
     gene            1449..1799
                     /locus_tag="VBC52_001590"
     CDS             1449..1799
                     /locus_tag="VBC52_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEO6883164.1"
                     /translation="MEGLSYELYPFNIYCKLIEPGGIQTDFWNRSLEITDGVKESPYS
                     TYAEKYLEDMTEPVSSNRSSPDLVAETIYIAATDNIKQWRYLVASEEFINYRKSMTDQ
                     EWIASMMKNYLGKD"
     gene            1805..2542
                     /locus_tag="VBC52_001591"
     CDS             1805..2542
                     /locus_tag="VBC52_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6883165.1"
                     /translation="MPLNQFFILNNGVKIPSLGFGVFQIDDPLECEQSVLDALQVGYR
                     LIDTASGYLNEEAVGRAIKKSGIPRKEIFVVTKLWIQDASEEKAGPAIDRALKRLQLD
                     YINLYLIHQPMGDYYGACRALEKAYEERKALAIGVSNFSNGRVQDLILHNKIIPAVNQ
                     IQMNPFHPAKDQVEWLSKKGIHTMAWGPFAEAKYGIFQHPTLVEIAERYNKNCVSNNP
                     SLGSEKQSNSHFKNCAQSSYYGKYGLF"
     gene            complement(3075..3605)
                     /locus_tag="VBC52_001592"
     CDS             complement(3075..3605)
                     /locus_tag="VBC52_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEO6883166.1"
                     /translation="MRKREMKLELYNNKWPEKYQEIKEELTKLLSGLEIECHHFGSTS
                     IKGMASKPIIDVLIFVNNINMVDTYNNSMLNSGYVAKGENGILGRRYFVKYAKDKINH
                     LVHLHFYEKEDPKGLQELQFRDYLIENKTAFDRYLAVKVEASKKYKDMPEAYQNHKST
                     VIDAINKEIVKTKVSK"
     gene            complement(3647..4438)
                     /locus_tag="VBC52_001593"
     CDS             complement(3647..4438)
                     /locus_tag="VBC52_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEO6883167.1"
                     /translation="MLPYYVVNAFTNKAFSGNPAGVVVLNEPIKEDLMQKIAKENKLS
                     ETAFILKEKDLYKIRWFTPEKEVDLCGHATLASTYVISRYLEDDLSHITFSSKSGLLE
                     VTISGEDITLFFTMVDVKQVPITAMMRESIEPTILEAYRTADEEHLVLYLENQESIEN
                     FKIDLEKISQLAPHGVTLTAQSSEKNIDYVLRYFAPNYGINEDPVTGSAQTRLAPIWT
                     KKLGKEQLVSKQLSERQGLMMIELEENLVKISGQANLYLKGNIDI"
     gene            complement(4448..4876)
                     /locus_tag="VBC52_001594"
     CDS             complement(4448..4876)
                     /locus_tag="VBC52_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007892498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6883168.1"
                     /translation="MQDEEVMWAYDKSFTDQDCQYWLDWNMNSYRSDGFGLWKIIHKE
                     TSEVVGECGITMQTVEGENYPEIGYHLKKEHWNKGIATEVARAVRDYAFEELNLEELI
                     IISRDINLQSMNIAIKSGFTIKKRFLKNDRFPHFMFYLKR"
     gene            5788..6087
                     /locus_tag="VBC52_001595"
     CDS             5788..6087
                     /locus_tag="VBC52_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEO6883169.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQKQADLAASQFTGKSAQELVTVTFT
                     GDKKLISIDYKEAVVDPEDIETLQDMTTQAINDALSQVDDATKKIMGAFAGKMPF"
     gene            6248..7438
                     /locus_tag="VBC52_001596"
     CDS             6248..7438
                     /locus_tag="VBC52_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6883170.1"
                     /translation="MVMDSLIIKQLIKSTFDIPLQVTYPNGNIETYNGNNPHVKLKLN
                     KNFSVSELSKDPSIVLGEAVMDGDIEIDGSIQELILSAYRCGDSFLRNSKFSKLIPKQ
                     FHDKKHSKSDIQKHYDIGNDFYKLWLDDTMTYSCAYFKHENDSLEQAQLNKVHHILNK
                     LNAHPGGKLLDIGCGWGTLIITAAKEYGLNATGITLSEEQASFITKRIKEEGLENKVT
                     VLIKDYRDIHETYDYITSVGMFEHVGKENLSQYFQTISKRLNINGLALIHGITGQVGG
                     NHGSGTNSWINKYIFPGGYIPRLTENLNHIASAGLQIADLEPLRRHYQKTLELWTKNF
                     HNALPEVQKTHDKRFINMWDLYLQSCAASFESGNIDIFQYLLSKGVSKDTMPMTRDYM
                     YSAN"
     gene            complement(7508..8233)
                     /locus_tag="VBC52_001597"
     CDS             complement(7508..8233)
                     /locus_tag="VBC52_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6883171.1"
                     /translation="MSEKYSTGDLAKEAGVTVRTVQYYDKRGILSPSELSEGGRRVYS
                     IADLEKLRQIIYLRDLDFSIDNIKNLFTEDNASQILELFLQVQIRELRLSIDSKKDKL
                     DKAVNLLKTVEKQDSKTLGYLSDIVLMEENKRKWGKLQKSIVLQVIMIIMVYITLIMA
                     AVSLNQRWLMWIAVTVFLIAMNGMVWYFKQQVVYLCPNCHQTFTPTYQAFNLAKHTPK
                     TRKVFCPHCHQKSHCIELAREER"
     gene            complement(8360..8947)
                     /locus_tag="VBC52_001598"
     CDS             complement(8360..8947)
                     /locus_tag="VBC52_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEO6883172.1"
                     /translation="MKQLQEYIAFDLEFNTVGEHSHIIQVSSVKYSNHQEIALFDTYV
                     HTKVPLQSFINGLTGITARDIIGAPKIETVLTDFQSFVGDTPLIGYNGHKSDLPLLVE
                     NGLDLTSQYQVDLYDEAFVRRSTDLNGIVNLKLTTVADFLGIKGKAHNSLEDARMTAR
                     VYEKFLDLDENKIYLKQQKEVAVDSPFATLGNLFD"
     gene            complement(8991..9527)
                     /locus_tag="VBC52_001599"
     CDS             complement(8991..9527)
                     /locus_tag="VBC52_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEO6883173.1"
                     /translation="MTVEKTVSELAEILGVSRQAMNNRVKTLPEECVEKNSKGVTVVN
                     RDGLIKLEEIYKKTILEEEPIDEEASRRELLEILVDEKNTEITRLYEQLKAKDIQIAS
                     KDEQLHVKDIQIAEKDKQLDQQQQLTLTAMEDTQRLQLELNEAKAEVEEIQEAKEEKI
                     QELEAVKKSFFGRFFNKK"
     gene            9662..10834
                     /locus_tag="VBC52_001600"
     CDS             9662..10834
                     /locus_tag="VBC52_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6883174.1"
                     /translation="MKHYDTIIIGGGPAGMMAAISSNFYGNKTLLIEKNKRLGKKLAG
                     TGGGRCNVTNNGNLDELLAGIPGNGRFLYSVFSQFDNHDIINFFQDNGVTLKEEDHGR
                     MFPTTDKSRTIINALENKIKELGGQIMTDTEVVSVKKIGDSFYIKTKDTQFASDKLVV
                     TTGGKSYPSTGSTGFGHDIARHFKLEVTDMEAAESPLLTDFPHKKLQGISLDDVTLSF
                     EKHIITHDLLFTHFGLSGPAALRISSFVKGGETIYLDVLPNISVKELETHFQNEREKS
                     LKNALKILLPERLAEFYAEDLPEKVKQVSVKDLETLIQKLKKLPILVTGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVAGLHFAGEVLDINAHTGGFNITSALCTGWVAGSLHY"
     gene            complement(10877..11659)
                     /locus_tag="VBC52_001601"
     CDS             complement(10877..11659)
                     /locus_tag="VBC52_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HEO6883175.1"
                     /translation="MCYNNSMNERYIRFNPNIAKQKPSNKDKCPFCDKSQLGKILDVK
                     DDMIWVENKYPTLEETYQTLVIESNDHNGDISVYSESKMRQLLDYLLSKWQLMEESGH
                     YRSVVLYRNFGPLSGGSLRHPHSQIVGLKNVDAYETLNLDWFDGIEVCSETSQQAEIS
                     IANRPMLDFTEFTIKVSKLENAILIADSLPILCHYILNEFLGGRCKSYNLFFYKHDGG
                     LICKIVPRFIVSPYQIGYAIHQVNLPDELEKIKEALRAKYYL"
     gene            11818..12087
                     /gene="rpsN"
                     /locus_tag="VBC52_001602"
     CDS             11818..12087
                     /gene="rpsN"
                     /locus_tag="VBC52_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEO6883176.1"
                     /translation="MAKKSKIAKFQKQQKLVEQYAELRRELKEKGDYEALRKLPKDSN
                     PNRLKNRDLIDGRPHAYMRKFGMSRINFRNLAYKGQIPGIKKASW"
BASE COUNT         4205 a         2228 c         1851 g         4162 t
ORIGIN      
        1 aaataagttt gcaactttat gttgtttaaa ttaaaataca aaaaaatgaa agtgtatgac
       61 actttcattt cattatagca cagaaaatat tttgaaaaat tggtcataaa gttacaatta
      121 atcgatcgaa aatgtcaact tagaatctgc aattgtttta ataaatccta atgttgatga
      181 acttggatta ttactataaa tcaaatcata aggtaaggag aattcactct ccaagggaat
      241 agttatcaag tttgggtgta catcaataat ttttgcctgg gaaaattcac ttaacaatgc
      301 atttctaaac tgatccattc ccgttaaggc atttttttga ggcattaata tactaaattc
      361 tacaatatca gaaagtgtta cctttttttg agagctaatg gatgattact attaaccgca
      421 actgctaaag gagtttgaaa gagttcgtag aattggcaat gtcccttcca agcaatatca
      481 caatatatcg cttcaactac atcacactca gcttccgaag tgtgcggaaa ggcaaagtca
      541 tagggaaaaa tgtgctcttt aaaataattg acaaatttca ttaacaaacg aggtttaaaa
      601 atatctgctg ttccaatttt aataatttct ttttgactag caatagatcg acctttctca
      661 atcgctagtt tagacatctt gataatatta agaccatccg tgtataaaga ctgacctgca
      721 ggtgttaaac tgataccggt tttccaataa attgatttgc tgtgcaacag cagaagttga
      781 aatataaagc ttttttgcag ccttgctaaa tgaaccttct tctgcaactt ttacaaacgt
      841 ttctaattgt tgaatataca tgtcatagtc tcctagatat catcaaatat aaagttttga
      901 cttaacgcta tcataacatt ttacaaattc ccaagcaaga gagcacttgt taaaatgaca
      961 ataagataag gagaaatatt atgacaaaaa caattgtaat tacagaagct tcttctggca
     1021 ttggagaagc aacagccaaa ttttttgcaa aaaaaggatg gcaagtagcc gcaacaatgt
     1081 gaagacccga aaaggaaaca gatctatctc aattagcaaa tatcaaagtt tttaagttag
     1141 atgtaactga ctctttttca attcaaaaag catttttaga aatcaaagaa gaatacaaaa
     1201 ctattgattg tttactaaat aacgctggtt atgcactctt tggcccttta gaattatcat
     1261 ctgaagaaca aatacgtcgg gcatacgata ctttatcgtt aggtgtaact tttgttacaa
     1321 aacaatttat tccactttta cgcgaaaatg ggggcggtag tattattaca atttcttcgg
     1381 taggaggtgt cctcacacta ccattaacag caacctacca cgcagcaaaa taggcagtgg
     1441 aaggattaat ggagggcctc agctatgaac tttatccctt taatatttat tgtaagttga
     1501 ttgaacctgg cgggatacaa acagattttt ggaaccgttc tttagaaata acagatggtg
     1561 taaaagaaag cccatattca acatatgcag aaaaatacct ggaggatatg actgaaccag
     1621 tctcttctaa tcgttcaagt cctgatttgg tagcagaaac tatttacatc gctgctactg
     1681 ataacattaa acaatggcgt tatcttgttg cgtcagaaga atttatcaac taccgtaagt
     1741 ctatgactga tcaagaatgg atagcatcaa tgatgaaaaa ttatttagga aaggattaac
     1801 cataatgcca ttaaatcaat tttttatatt aaataatgga gtaaaaatac caagtcttgg
     1861 ctttggcgtt ttccaaatag atgaccctct agagtgcgaa caatccgtct tagatgcact
     1921 tcaagttggt tatcgcctca ttgacacagc ttctggatac cttaatgagg aggcagttgg
     1981 tcgtgctatt aagaaatcag gaatccccag aaaagaaatt tttgtcgtga ctaaactttg
     2041 gatccaagat gcatctgagg aaaaggctgg acctgctatt gatagagcat taaaaaggtt
     2101 acaactagac tatattaacc tttatttaat ccatcaacca atgggggatt attatggggc
     2161 atgtcgtgca ttagaaaaag cctacgagga gagaaaagct ctagcaattg gtgtttctaa
     2221 tttttcaaat ggacgagttc aagatttaat cttacataac aagattatcc ctgcggtcaa
     2281 tcagatacaa atgaaccctt ttcatcctgc aaaagatcaa gtagagtggt taagcaaaaa
     2341 gggcatccat actatggctt ggggaccatt tgcagaagcg aaatacggaa tctttcagca
     2401 tcctactttg gtagagattg cggaacgcta taataaaaac tgtgtctcaa ataatcctag
     2461 cttgggaagt gaaaaacaaa gtaacagtca tttcaaaaac tgtgcacaaa gctcgtatta
     2521 tgggaaatat ggactttttt gacatattat taaccgatga tgatatgact aaaatagcaa
     2581 gccttgaaaa atcagaatat ctatttggtg atgtagaatc actggagcta attgaatact
     2641 tatcaaataa tgtttttgat atatagaaat tcttcttcat cgctaaaata agaattatct
     2701 tgtacatttg tagaatataa acattgaata acactatctg aatctctttt tagccttgcc
     2761 ccctccttgc tctaatctat tagagcaaat tattttaatt gcttttaata aggagaatga
     2821 gatcttatga tgactatgca aggctcgttc tacgtttctt tatgctatgt aatagtcaga
     2881 gatatttatt taaggcctta cataatttta taaattaatg taaaatcatt taaacctaca
     2941 tcaatatgat gtaattagtc aatgacagta tcttctacac aataaaaatc ggtttacaga
     3001 aaaatgtaaa acgatttttt attacctata aattttctct taagtcgaac agttaattaa
     3061 tttactatca aaatttattt agagactttt gtctttacta tctctttatt aatggcatca
     3121 ataaccgtag acttatgatt ttgataggct tctggcatat ctttatattt cttactcgct
     3181 tcgactttta cagccaaata acgatcaaaa gcagtcttat tttctattaa gtaatctcta
     3241 aattgtaact cttgtaaacc tttcggatct tccttttcat aaaaatgtag gtgtactaag
     3301 tgatttattt tgtcttttgc atatttaaca aaatatcttc ttcctagaat tccattttca
     3361 cctttagcaa catatccaga attcaacatg gaattattat acgtatctac catattaata
     3421 ttgttaacga atatgagtac atcaattatc ggtttagagg ccataccttt gattgaagtt
     3481 gacccaaaat gatggcattc aatttctagt ccacttaata gtttagtaag ttcttcttta
     3541 atttcttgat acttctctgg ccatttatta ttatatagtt cgagtttcat ttctcgtttt
     3601 ctcattaata tactcctact tcactaaaat tgttaacttt caagtactaa atatcaatat
     3661 tgcctttaag atataaatta gcttgtcctg atattttaac aagattctct tccaactcaa
     3721 tcatcatcaa cccttgtctt tcagataatt gcttggatac taattgttcc tttccaagct
     3781 ttttcgtcca aattggtgct aaacgtgtct gtgctgagcc agtgacagga tcttcattga
     3841 taccataatt tggtgcaaag taacgaagaa cataatcaat atttttttct gaactttggg
     3901 ctgtaagtgt cactccatgt ggtgctaatt gactaatttt ctctaaatca attttgaaat
     3961 tctctataga ttcttgattt tccagatata aaacaagatg ctcttcatct gctgttcgat
     4021 aagcttccag gatagtaggt tcaatcgatt ctctcatcat cgctgttatt ggaacttgct
     4081 taacgtcaac cattgtaaaa aataaggtta tatcttcacc actaatagtt acttctagaa
     4141 gtcctgactt tgaagagaaa gtgatatgac ttaaatcatc ttcaagataa cgtgaaataa
     4201 cataggtact tgctagagtc gcatgcccac ataaatccac ttctttttct ggtgtgaacc
     4261 atctaatctt atatagatct ttttctttta aaataaatgc agtctcagat agtttatttt
     4321 ctttagctat tttctgcatt aaatcctctt taatcggttc atttaaaacc acaacccccg
     4381 cagggttgcc actaaacgcc ttattggtaa aagcattcac aacataataa ggtaacatat
     4441 cacattccta tcttttcaag taaaacataa aatgaggaaa cctatcattc ttcaaaaatc
     4501 tttttttaat agtaaatcct gattttatgg caatattcat tgactgtaaa ttaatatctc
     4561 gacttatgat aatgagttcc tcaaggttta actcttcgaa agcataatct cgtactgccc
     4621 tagcaacttc tgtcgcaatt cctttattcc aatgctcttt tttaagatgg taacctattt
     4681 cagggtaatt ttctccttca acagtttgca ttgtaatgcc acattcacct acgacctctg
     4741 aagtttcttt atgaatgatt ttccacagac caaatccatc agacctgtaa gaattcatat
     4801 tccaatctaa ccagtactga caatcttgat cagtaaaact cttatcataa gcccacatca
     4861 cttcttcatc ttgcaaaatg actttaactc agataaatct ccgctatccc attctaataa
     4921 aataaatcga ctagtttcta aaattatatt cttcataatt catccttata gattaaatat
     4981 tacaaatttc tttacattct caattattat ccaaactaac tacagccgta aactagctgt
     5041 ttaaaaatat tttaattata ttatataatt tttatctcaa ataactacta aaaaagcgtc
     5101 attgtgagac ttttaagtcg gaattaatat aaattataac agcacaaagt gcaaaatatc
     5161 ttttctaaac agaaaaatca ataaatctaa aaaataacta gcaaataaaa gctgtctgat
     5221 tagttattat ctcatgatat ttctggtcat tttaatatcc caatactaac taaaaaagga
     5281 atgaaaaata cctagacact taaatgcggt tgaaaaactt aatgcatcaa taacattacc
     5341 cattaatggt aaaataaaag ccaaaagaag taatcctcaa aaactaaata attcacttgc
     5401 tcctagatga gcgatgttac tttttaggag tgtttaaaca tatatttaca cttttttaaa
     5461 atatctagtt aagagtcata tctgactaaa caattactga accttcatct ttccatcatt
     5521 tgaacagtta aactattcaa atctccttct atataaaaaa tcttgtaaat atttttttat
     5581 cgttaattat cttattatag aataatttat ttttgaaaca attaagaatc ttactacaat
     5641 cttggaaaca ttctttctct tggtaattta gccaaatgaa ctagtataga ccttcaaaaa
     5701 tccaatatta tctaatccct atgacacacc ccctttccta tgctataatg ttacagtaat
     5761 cacttttact aaggagaata aaatcttatg atgaatatgc aaaatatgat gcgtcaagca
     5821 caaaagcttc aaaaacaaat ggaacaaaag caagctgatt tagcagctag ccagtttact
     5881 ggaaaatctg ctcaagagct cgttactgtg acatttacag gcgataaaaa gctaatttca
     5941 attgattaca aagaagctgt ggtagaccct gaagatattg aaacactaca agacatgaca
     6001 actcaagcta tcaatgacgc tttaagccaa gttgacgatg ctacaaaaaa aattatggga
     6061 gcatttgcgg ggaaaatgcc attctaaaaa ataaatcaca tttactgaat cacctgtaaa
     6121 tgtgattttt atttcaaact tctttcattt tttcacgaaa acaagtttta ttttcaaaaa
     6181 aatatttgca aaaaaccagc agagaggtat aataacattg aaaatattag agagaaggtg
     6241 actaaccatg gttatggata gtctgatcat aaaacagttg attaagagta catttgatat
     6301 acccttacaa gtcacttatc caaatgggaa tattgaaact tataatggta acaatcccca
     6361 cgtcaaactt aaactaaaca aaaatttctc cgtctcggag ctatcaaaag acccttctat
     6421 agtattgggt gaagccgtta tggatggaga cattgaaatt gatggttcta tacaagaatt
     6481 aattttatca gcttaccgct gtggagacag ctttctacgt aatagtaaat ttagcaaatt
     6541 gattcctaaa caatttcatg ataaaaaaca cagtaaatcc gacattcaaa agcattatga
     6601 catcggaaat gatttctata aactttggct tgatgatacc atgacttatt catgcgccta
     6661 ttttaaacat gaaaatgaca gtctagagca agctcaacta aacaaagtac accatattct
     6721 taataaatta aatgctcatc cgggagggaa attactagat atcggttgtg gctgggggac
     6781 acttatcatt actgctgcta aagaatatgg cttaaatgcg acaggtatca ccttaagtga
     6841 agagcaggct tccttcatta ctaagcgtat caaggaagaa ggattggaaa ataaagtaac
     6901 agtcctcatt aaagattatc gtgacattca tgaaacatac gattatatca ctagtgttgg
     6961 tatgtttgaa catgtaggaa aagaaaattt atctcaatac ttccaaacta tttctaaaag
     7021 actcaacatt aatggtctag ctcttattca tggtattact ggacaagtgg gtggtaatca
     7081 tggtagtgga actaattctt ggattaataa gtatatcttc ccaggaggat atatccctcg
     7141 tttaactgaa aatttaaatc atatagctag tgctggactt caaattgctg acttagaacc
     7201 gcttcgtcgt cactatcaaa agactctcga attatggaca aaaaatttcc ataatgccct
     7261 tcctgaagtt cagaaaactc atgataagcg ttttatcaat atgtgggacc tctatcttca
     7321 atcttgtgcg gcctcttttg aatctggcaa tattgatatt tttcaatact tgctttcaaa
     7381 aggtgtttca aaagacacta tgcctatgac acgcgattat atgtattctg ctaattgata
     7441 tagttaatat tatagtattc caacttattc taaataaaaa gaactctctt gaattcaagg
     7501 gagttcttta acgttcttct ctggctaact ctatacaatg tgacttttgg tgacaatgtg
     7561 gacagaatac ttttcgcgtc ttaggggtat gtttcgctag attaaatgct tgatatgtcg
     7621 gtgtaaaggt ttgatgacaa tttggacaaa gataaacaac ctgttgttta aaataccaaa
     7681 ccataccatt catagctatt aagaatactg ttactgctat ccacataagc catctttgat
     7741 ttagagaaac tgctgccatt attaacgtaa tatagaccat aataatcata atgacttgta
     7801 gcactatgct tttttgtagt ttaccccatt ttcttttgtt ttcttccata agcacaatgt
     7861 cagaaagata gcctaatgtt ttactgtctt gtttttctac ggtttttagt aggttgacag
     7921 ctttgtcaag tttgtctttt ttactatcaa tagatagcct taattctctt atctgcactt
     7981 gtaaaaaaag ctctaaaatc tgactagcat tgtcttcagt aaatagattt ttgatattat
     8041 caattgaaaa atctaaatct cgtaaataaa taatttgtct aagcttttct aaatcagcaa
     8101 tactataaac cctcctacct ccttcagata gttctgatgg ggataaaatt cctcttttgt
     8161 catagtactg aacagtacga acagtaactc ctgcttcttt agctaagtca ccagtggaat
     8221 atttttctga catgtttcac acctccttta atatagtaaa cacttcttta tcagaaacac
     8281 ctttactata tcaaatgacc taaggtaata agcaagtaat aacgctaagt atttttaact
     8341 ttaaataaaa ctacaacaat taatcaaaca aatttcctaa agtcgcaaat gggctatcaa
     8401 ctgctacctc tttttgctgt tttaaataga ttttattttc atccaagtcc agaaattttt
     8461 cataaacacg cgcagtcatg cgggcatcct ctaagctatt atgtgcctta ccctttattc
     8521 ctaaaaagtc agcaacagta gtcaatttaa ggttgacaat accatttaaa tctgtactac
     8581 gtctcacaaa agcttcatca tataagtcca cctgatattg actagttaga tccaaaccat
     8641 tttcaactaa aagaggtaaa tccgatttat gcccattata accaatcagc ggtgtgtctc
     8701 caacaaaact ctgaaagtct gtcaaaactg tctcaatttt aggagcccct ataatatctc
     8761 tagctgtaat acctgttaaa ccattaataa agctttgtaa tggaactttt gtgtgaacat
     8821 aagtatcaaa taaagcaatc tcttgatgat tagaatattt aacggacgac acttgaataa
     8881 tatggctatg ctcaccaacc gtgttaaact ctaaatcaaa agctatatat tcttgtaatt
     8941 gtttcattat ttctcctaaa aagcgcaagg aaactcctta cgcttttatt ctatttttta
     9001 ttgaagaaac gtccaaagaa acttttttta acagcttcta attcttgaat tttttcttct
     9061 ttggcttctt gaatctcttc aacctctgct ttagcttcat ttaactcaag ttgcagacgt
     9121 tgcgtatctt ccatagcagt taaagtcaat tgctgttgtt gatctaattg cttatctttc
     9181 tctgctattt gaatatcttt tacatgtaac tgctcgtctt ttgaagcaat ctgaatatct
     9241 tttgctttta actgttcata taaacgtgtg atttcagtat tcttctcatc aacgagaatt
     9301 tctaaaagtt ctcgtcttga tgcttcttca tcaattggtt cctcttccaa aatagttttt
     9361 ttatagattt cttccaattt aatcaagcca tcacgattaa caactgtaac gcctttacta
     9421 ttcttttcca cacattcttc agggagtgtt ttaactctgt tattcatcgc ttgacgacta
     9481 acccctaaaa tttcagctaa ttcactaacc gtcttttcaa ctgtcataac ttcctctttt
     9541 gtactgattt tcttataaaa gaatatcaca atcctaatct agtgtcaatt tactgtcaac
     9601 ttaaagtgtc aaacaagaca ttttgacgtc tatttctcta tttatatgct agaataagat
     9661 tatgaagcat tatgacacaa taattatagg tggtggacct gctggaatga tggcagctat
     9721 ttcctcaaat ttttacggca acaaaacact tcttattgag aaaaataaac gtttaggcaa
     9781 aaaactagct ggaacaggtg gtggccgctg taatgttacc aataatggaa accttgatga
     9841 gctcttagct ggtatacctg gaaatggccg tttcctatat agcgtcttct cacaatttga
     9901 taaccatgat attattaact tctttcaaga caacggcgtc acacttaaag aagaagatca
     9961 tgggcgtatg ttccctacta ctgataaatc tcgtactatc attaacgccc ttgaaaataa
    10021 aataaaagaa cttggtggtc aaattatgac tgacacagaa gttgtctctg tcaaaaagat
    10081 aggagattca ttctatatca aaactaaaga tacacagttc gcatccgata aacttgtcgt
    10141 aacaactggt ggtaaatcct atccgtctac aggttctact ggtttcggtc atgacattgc
    10201 tcgtcacttt aaattagaag tcacagatat ggaagccgcc gaaagccctc ttttaactga
    10261 ttttccccat aaaaaactac aaggtatctc cttagatgat gtcactttgt catttgaaaa
    10321 gcatattatt acacacgatc ttctctttac tcattttgga ttatcaggac cagcagcctt
    10381 gcgaatttcc agctttgtca aaggcggaga aactatatac ctagatgttt tgcccaatat
    10441 ctctgttaaa gaattggaaa cacacttcca aaatgaaaga gaaaaatctt tgaaaaacgc
    10501 tcttaaaata cttcttcctg agcgtttagc tgaattttac gctgaagact tacccgaaaa
    10561 agtaaaacaa gtatctgtaa aagacttaga aacgttaatt caaaaactta aaaaacttcc
    10621 tattttagta actggcaaga tgtctctagc aaaatccttt gtaacaaaag gtggagttga
    10681 tcttaaagaa ataaacccca aaacacttga aagtaaaaag gtagctggtt tacattttgc
    10741 tggtgaagtc ctagacatca atgctcatac gggagggttt aacattactt ctgccttatg
    10801 tacaggctgg gtagctggta gcttacacta ttaatacatg aaagctgaac aattccgtca
    10861 gctttttata tagaatttaa agataatatt tggctcttaa ggcttctttg attttttcca
    10921 attcatctgg taaatttacc tgatgaatag catacccaat ctgataaggg gatactataa
    10981 aacgaggaac aattttacaa ataagacccc catcatgctt atagaaaaag agattataag
    11041 atttacagcg ccctcccaag aattcattta aaatataatg acacaaaatt ggtaaactat
    11101 ctgcaatcaa aatagcgttt tccaatttgg atactttaat tgtgaactct gtgaaatcaa
    11161 gcataggacg attggcaata ctaatctctg cttgttgact cgtttccgaa caaacctcga
    11221 taccatcaaa ccaatctaag ttcaacgttt cataagcatc tacgtttttt aagccaacaa
    11281 tttggctatg aggatgtcgt aaacttccgc cagataaggg cccaaaattt cgataaagaa
    11341 cgaccgaacg gtaatgacca ctttcttcca ttaattgcca ttttgacaaa agataatcta
    11401 acaattggcg catctttgac tcactataaa ctgaaatatc cccattgtga tcatttgatt
    11461 caatcaccaa tgtttgatag gtttcttcta aagttgggta tttattttct acccagatca
    11521 tatcgtcttt cacgtctagg atttttccta attggctttt atcacaaaat ggacatttgt
    11581 ctttattact gggtttttgt ttcgcgatat tagggttaaa tcggatatat ctttcattca
    11641 tactgttatt gtaacacatt catcaccatt acaatataat acttatccag ttaacatttt
    11701 ccttgcattc ttagctgaaa agagtataat agaatcatgt cttgtttact ggttaattac
    11761 cagttaacta ggcaccctgt tttgggatag aaaaatcttt ttgggaaagg agaaatcatg
    11821 gctaagaaat caaaaattgc taaatttcaa aaacagcaaa aactcgtaga acaatatgct
    11881 gaactacgtc gtgagttaaa agaaaagggt gattacgaag cacttcgtaa acttcctaaa
    11941 gattctaacc ctaaccgact taaaaatcgt gatttaatcg atggccgtcc tcacgcttac
    12001 atgcgtaaat tcggtatgag tcgtattaac ttccgtaacc tagcttacaa aggccaaatt
    12061 cctggcatta aaaaagcaag ctggtaattc gtgaagacaa atagttactt tttatgacta
    12121 ttctattgag tgtggacgag tagttaatac attgtaatta tttaacctgt tgagtattta
    12181 ctcagctatt acaaacttct tcaaaatttt attatcatag gaatgtagga tctcatcaat
    12241 ccttaaatga ctaataataa aagatgaaaa aatttttctt atctcaaatc atagatgcca
    12301 atttatttat tttcaaaaag cccggtatgc caagataccg agctttttat tattataaat
    12361 actgataaaa ttcttttagt taattcccaa aaacaagact attatttgta atagtcacta
    12421 ctgaataata tttcttttat taaaaa
//
LOCUS       DATRNK010000057        12424 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.057, whole genome shotgun sequence.
ACCESSION   DATRNK010000057 DATRNK010000000
VERSION     DATRNK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12424
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.057"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            42..741
                     /locus_tag="VBC52_001603"
                     /pseudo
     CDS             42..741
                     /locus_tag="VBC52_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106739.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            796..1422
                     /locus_tag="VBC52_001604"
     CDS             796..1422
                     /locus_tag="VBC52_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883177.1"
                     /translation="MEQSEKKVLAIIAIVIGAIALISSWMPFINNGSFVIAIIALIIG
                     VFALFFNRKRKKTLTYVSIIISILAMIIVLVTQSMYGKVIDTASKSFDKTSKSYETSY
                     SKSSSIEASSSRAKVKNADTKFKWSESYFNSLVKGQTTYDEVVAKVGKPNSVSDSTDY
                     DIETDAEVPSKDCGWDLNDGSYYANVSIHFIQKNGVLVIDSKSSNGLK"
     gene            1550..2606
                     /locus_tag="VBC52_001605"
                     /pseudo
     CDS             1550..2606
                     /locus_tag="VBC52_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(2652..2723)
                     /locus_tag="VBC52_001606"
     tRNA            complement(2652..2723)
                     /locus_tag="VBC52_001606"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2688..2690),aa:Arg,seq:ccg)
     gene            complement(2796..3998)
                     /gene="rpsA"
                     /locus_tag="VBC52_001607"
     CDS             complement(2796..3998)
                     /gene="rpsA"
                     /locus_tag="VBC52_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEO6883178.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIDGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVVEESAAAARKEVFSNIEVGSVVTGKVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVTVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AAGDVIEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSAGQEVTVKVL
                     EVNSDAERVSLSMKALEERPAQAEGEKEEKRQSRPRRPRRQEKRDYELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(4124..4195)
                     /locus_tag="VBC52_001608"
     tRNA            complement(4124..4195)
                     /locus_tag="VBC52_001608"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4161..4163),aa:Gln,seq:ttg)
     gene            complement(4201..4281)
                     /locus_tag="VBC52_001609"
     tRNA            complement(4201..4281)
                     /locus_tag="VBC52_001609"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4245..4247),aa:Tyr,seq:gta)
     gene            complement(4342..4572)
                     /locus_tag="VBC52_001610"
     CDS             complement(4342..4572)
                     /locus_tag="VBC52_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001870505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEO6883179.1"
                     /translation="MSKKDKKIEIQISDAQVTVNGTKVDGYQLVIGKKIVGQIAELDS
                     KFAVIKGEQVSGFYKNLDQAMGNIIEEYNLNR"
     gene            complement(4655..5677)
                     /locus_tag="VBC52_001611"
     CDS             complement(4655..5677)
                     /locus_tag="VBC52_001611"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEO6883180.1"
                     /translation="MTVNLDWDNLGFAYRKLPFRYISHFKDGKWDDGKLTDDATLHIS
                     ESSPALHYGQQAFEGLKAYRTKDGSIQLFRPDQNAERLQRTADRLLMPHVPTDKFIAA
                     VKSVVRANEEFVPPYGTGATLYIRPLLIGVGDIIGVKPAEEYIFTVFAMPVGSYFKGG
                     LTPTNFIVSKEYDRAAPNGTGAAKVGGNYAASLLPGKYAHEKQFSDVIYLDPATHTKI
                     EEVGAANFFGITKDNQFITPLSPSILPSITKYSLLYLAKERFGMEAIEGDVFVDELDK
                     FTEAGACGTAAVISPIGGIQNGDDFHVFYSETEVGPATRKLYDELVGIQFGDVEAPEG
                     WIYKVD"
     gene            complement(5790..8249)
                     /gene="parC"
                     /locus_tag="VBC52_001612"
     CDS             complement(5790..8249)
                     /gene="parC"
                     /locus_tag="VBC52_001612"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEO6883181.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGFRKSAKSVGNVMGNFHPHGDSSIYDAMVRMSQDWKNRETLIEMH
                     GNNGSMDGDPAAAMRYTEARLSEIAGYLLQDIDKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGATGISAGYATDIPPHNLAEVIDAVVYMIDHPKAKLDKLMEFLPGPDFPTGAI
                     IQGKDEIRKAYETGKGRVAVRSRTAIETLKGGKKQIIVTEIPYEVNKSVLVKRIDDVR
                     VNNKVPGIAEVRDESDRDGLRIAIELKKEADETIVLNYLFKYTDLQVNYNFNMVAIDD
                     YTPKQVGLSRILTSYIAHRREIIIARSKFDKEKAEKRLHIVEGLIRVLSILDEVIALI
                     RASENKADAKENLKVSYEFSEAQAEAIVTLQLYRLTNTDIVTLREEEEELRQQITMLK
                     AIISDERTMYNVMKRELREVKKKFANTRRSELQELAETIEIDTASLIIEEDTYVSVTR
                     GGYVKRTSPRSFNASTVDELGKREDDELIFVSNAKTTQHLLMFTNLGNLAYRPVHELA
                     DIRWKDVGEHLSQNLVNFASNEEIIYAELVDDFTKETYFAVTSLGQIKRFERQEISPW
                     RTYKSKTAKYAKLKNVDDYVVTVAPIQLEDVILVTYNGYALRFSINDVPVVGSKAAGV
                     KAMNLKDSDHIVSAFIANTTSLYLLTHRGSLKRMAIDVIPTTSRANRGLQVLRELKSK
                     PHRVFKAGPVYLEDSSFEFDLFSSVSNHEGDTFVLEIMSKTGKVYDVDLSQWSFSERT
                     SNGSFVSDKISDEGVFSVKIK"
     gene            complement(8383..10332)
                     /gene="parE"
                     /locus_tag="VBC52_001613"
     CDS             complement(8383..10332)
                     /gene="parE"
                     /locus_tag="VBC52_001613"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEO6883182.1"
                     /translation="MAKQDITVTNYGDDAIQVLEGLDAVRKRPGMYIGSTDGTGLHHL
                     VWEIVDNAVDEALSGFGNRIDVIINKDGSITVTDHGRGMPTGMHAMGKPTVEVIFTVL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEIIRDGAIYRQRFENGGKPVTTLK
                     KIGTAPKSKSGTSVSFMPDQSVFSTIDFKFNTIAERLKESAFLLKNVTLTLTDNRSEE
                     AEHLEFHYENGVQDFVEYLNEDKETLTPIMFFEGEEQEFHIEVALQYNDGFSDNILSF
                     VNNVRTKDGGTHETGLKSAITKSMNDYARKTGLLKEKDKNLEGSDYREGLSAILSILV
                     PEEHLQFEGQTKDKLGSPLARPIVDGIVSEKLTYFLMENGDLASNLIRKAIKARDARE
                     AARKARDESRNGKKSKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTAKAKMADIIKNEEINTMIHTIGAGVGPDFNIDDINYDKVIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVEEGHVYIALPPLYKMSKGKGKKEIVEYAWTDIEL
                     EELRQKFGKGSLLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLM
                     GDKVPPRRQWIEDNVKFTLEENTVF"
     gene            10446..11084
                     /gene="plsY"
                     /locus_tag="VBC52_001614"
     CDS             10446..11084
                     /gene="plsY"
                     /locus_tag="VBC52_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEO6883183.1"
                     /translation="MSIIIMIIIAYLLGSIQTGLWIGKYFYQVNLRQHGSGNTGTTNT
                     FRILGVKAGIVTLTIDILKGTLATLIPIMLGVTTVSPFFIGFFAIIGHTFPIFAQFKG
                     GKAVATSAGVLLGFAPSFFLYLLVIFLLTLYLFSMISLSSITVAVVGILSVLIFPLVG
                     FILTDYDWIFTTVVILMALTIIIRHQDNIKRIRKRQENLVPFGLNLSKQRNK"
     gene            complement(11150..11803)
                     /locus_tag="VBC52_001615"
     CDS             complement(11150..11803)
                     /locus_tag="VBC52_001615"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEO6883184.1"
                     /translation="MKHSSWHDLIKRELPNHYYNKINTFMDAVYESGIVYPPRDKVFN
                     AIQITPLENVKVVIIGQDPYHGPQQAQGLSFSVPDNLPAPPSLQNILKELAEDIGSRS
                     HHDLTSWAQQGVLLLNACLTVPEHQANGHAGLIWEPFTDAVIKVVNQKETPVVFILWG
                     GYARKKKSLIDNPIHHIIESPHPSPLSAYRGFFGSRPFSRTNHFLEEKGINEIDWLN"
     gene            complement(11902..12387)
                     /locus_tag="VBC52_001616"
     CDS             complement(11902..12387)
                     /locus_tag="VBC52_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883185.1"
                     /translation="MRETETHDHQALIQKLLVSIHYLTLFRDEIILVEKTPSLLGKHF
                     SIAIVQNELGEILSKIEALSKQKKLIRSIYWYDESSFKVMNKALAIVEEWIKGLDNLL
                     EFCQSQTVFQAILGDERAHVFGILIDVYTSLNIINTSLKEENSRPVSLSDLALGLKDE
                     D"
BASE COUNT         3999 a         2404 c         2004 g         4017 t
ORIGIN      
        1 tcgaaaggaa aaaattatga caggaggaag atcctcaaga aatagagaca ttaaggttct
       61 tttcgagcaa cctaaatgat ttgctcaatg aaacaagtac aaagcaagtc gaactctcaa
      121 aggctacagg aataccacca agcactctta caggttatgt taaaggaaca cctttaccta
      181 ttccaggtaa cgtacaaaaa atagcagatt attttgaatt aatgaagtct gatatagacc
      241 caagatttgc tcctcgtaag aaaaatcatg aactcaaaat tcctacttct ccgctcgtta
      301 acaaaattac taaggcggtt gtagaattaa atgttcacgc aaacaacaag tactagatta
      361 tgctacagag caactaaaag agcaaaaaat aaaataacct ccatagaaga aaagttgtac
      421 gaatataagg tatatgaaaa actctctgta tgatgagcaa ttagactatg attttgcttc
      481 ttggttcttc ggtgattcaa tggaaccggc ttatcttaat ggcgaagttg cactaataaa
      541 gcaaacaggt tttgattacg atggagctat atacgctatc gagtggaatg gacaaactta
      601 gatcaaaaag tatataaaga agaagacggt ttaagattgg tatctataaa taagaagtac
      661 aaagataaat ttgctagttg gagtgaaaat ccaagaatta ttggaaagat tgttggacac
      721 tttacaccag aagaatatta gtattaaata atgtgcaatg cctgatccac gtaaaaagct
      781 ggtagggaga tttttatgga acaatctgaa aagaaagttt tggctattat tgcaattgta
      841 attggcgcaa ttgcgttaat tagctcatgg atgccattca ttaataacgg ctcgtttgtt
      901 atcgctataa ttgcgttaat cattggagtt tttgcgttat tttttaatag aaaacgcaaa
      961 aaaacactaa cttatgttag tatcattatt tcaattttag caatgataat tgttctagtt
     1021 acacaatcaa tgtatggtaa agtgattgat accgcaagca aatcattcga taaaacatct
     1081 aaatcatacg aaacctctta tagcaagtct tcttctatcg aagcttcttc ttctagagca
     1141 aaagttaaaa atgcggacac taaattcaaa tggtcagaat cttattttaa ttcgttagtc
     1201 aaaggtcaga caacttacga cgaagttgtt gcaaaagttg ggaaacctaa tagcgtatct
     1261 gacagcacgg actacgatat cgaaacagat gctgaggtac cttcaaaaga ttgtggttgg
     1321 gacttgaatg atggttcgta ctatgcaaac gtatctattc attttattca aaaaaatggc
     1381 gtactagtta ttgattctaa atcaagcaac ggattaaaat aaatgataaa caaaaagccc
     1441 cacgctcaaa ttttggtcaa ggaaagcgtg aggcaagata atataagaaa taagcattaa
     1501 atggcttgtt ttcttgtacc tattttagca aatttagaaa ggtttagcaa tggcgtattt
     1561 ccgtaaacgc gacaacaggt gggagtatcg gatatcgtat aaagatgtaa ctggtaagta
     1621 ccgacaaaaa tcaaagagcg gttttaaaac aaaaaaaaca agctgaactt gaagcaaata
     1681 agatagagat agaattatct aaaagatttc aaataaataa taatatagaa ttttgtactt
     1741 actttaaaaa ttggtctgaa ctttataaaa agccaaatgt aactgctact acttgggata
     1801 agtacaacta tactttgtct aagataaaag ggtatttcga taaagccaaa ttatcatcaa
     1861 ttaaaccaag tcattaccaa aaatttatta gtgattattt aaaccagtat aactggcaaa
     1921 ctattaaaat gataaatact cacatcagac aatcaataaa aatagctatc catgaaggtt
     1981 atattactaa agatttcact gctttcgtca tccttccgaa accagatgat agtaatgtag
     2041 atagtaaact tttagagcta tcagaatatc aaaaattaat ttcagagact agtaaagaag
     2101 tgaaatataa gtcacatttt tgtatttatt taatagcaac aactggatta agattttcag
     2161 aggctatggg attgacttgg agcgatatag atagagatga gttgctacta catgtcaaca
     2221 agacttacaa agtgtttgga agtgataaag gatttcaacc aactaaaaat aaacactcaa
     2281 ttagatatgt accaataaat aacacaacta tcaaattgct agatcaatat aggttgttat
     2341 tcaaaacaga aacaaggata tttgaaggaa tatcaaatac agctgtcaac aagacactaa
     2401 aaaaattagt tggcagaaat atacacattc attccctaag acatacttat gtgtctcatt
     2461 taatatcaga aggaatcgac ttgtttgcta tttccaagat agttggacac aaagatttaa
     2521 atatcacact aagcacgtat gctcatttgc tggaagatac aaaaaacaag aacaatgaaa
     2581 ttattaggga attatttggg gcataaacta atataaaccc tataacataa agctttacgg
     2641 agcatctatt atccatctta agggaatcga acccctatcc caagaaccgg aatcttgtgt
     2701 gatatccatt acactaaaga tggtagtaca tgttacatta tagcatactt tatcaacaaa
     2761 aacaaaaaga cttgctaaag caagtctttt aattattata attcaatatc accgaataag
     2821 tcagccatag agaatccagt ttgtgtttct ggaagctcgt agtcgcgttt ttcttgacga
     2881 cgtggacgac gtggacgtga ttgacgtttt tcttcttttt caccttctgc ttgcgctgga
     2941 cgctcttcaa gagctttcat tgaaagtgat acacgttctg catctgaatt aacttcaaga
     3001 actttaacag taacttcttg tcctgcagat aatacgtctt taggattttc aacacgtttg
     3061 tgtgaaattt gtgaaatgtg tacgagtcca tcaatgccag gtaacacttc aacaaaagca
     3121 ccaaagtcag tcaagcgttt tactttacct tcaataacat caccagcagc aagtttttgt
     3181 tcaacgccat cccatgggcc aggtgtagtt gctttaagtg aaagtgatac acgaccagct
     3241 tcttcatcaa ttgaaagtac tttaacttca acttcttcac caacagttac aactgattta
     3301 ggtgaaacat tgcgctcatg tgataattca gttacgtgta caagtccatc aacaccacca
     3361 aggtcaatga aagcaccaaa gcttgttaaa cgagcaactt taccagttac aactgaacct
     3421 acttcaatgt ttgagaaaac ttctttacgt gctgcagctg cagattcttc aacaacttca
     3481 cgccgtgaaa ggatgaagcg attttctgct gcatcaactt ctttgatttt agcatcaaat
     3541 tcttgtccaa caaatttttc agtgttacgt acaaaacgag tatcaatcat tgaagctggg
     3601 atgaaaccac gaagtccttc aaattcaact gaaagtccgc ctttaacagc acgtgtacct
     3661 ttaacagtaa caacttcacc ttcacgacca acaagtttgt cccatgcttt acgagcttcc
     3721 aagcgttttt tagatacaag gaaagttact gtatcagtat ctttacctac aacttgacga
     3781 agaacaagta cttcaactgt gtcgccagct ttaacaaaat cattgatatc agcatcacga
     3841 tcgttagtca attcacgaag tgtcaagaca ccttcaacac cagtaccatc gataacaacg
     3901 tttgcttgac cgttatcaac tgttaaaact tccgcagtga caacgtcacc agggttcact
     3961 tcgctaacac tgtttagcaa atcttcaaat tcattcattc taaaaaatcc tccaacaaaa
     4021 gctagccgag ctagcttttg ataacaatat attttctcaa ggtaacccgc aaggacaaca
     4081 aatatctctt tgacggtgtc aaacaaaagt ttgatacctt tgactggggt agctggattc
     4141 gaaccaacgc atgagggagt caaagtccct tgccttaccg cttggctata ccccaaataa
     4201 tggaaagaga gggattcgaa cccccgaacc cgaaggagcg gatttacagt ccgccgcgtt
     4261 tagcctcttc gctatctttc cgtaacaaca ctgactattc taccacagat gttgttagca
     4321 cgcaagcttt ttaaataaat attagcgatt aagattatat tcttcaataa tatttcccat
     4381 agcttgatct aaatttttat agaatccact cacttgctca cctttaatca cagcaaattt
     4441 agaatctagc tctgcaattt gacctacaat tttttttcca ataacgagtt ggtatccatc
     4501 aaccttagtt ccattaactg ttacttgagc gtctgaaatt tgaatttcaa ttttcttatc
     4561 ttttttactc actattaact ccaatatctc ttttattgtt ttttattgta cacaaaatac
     4621 taaaagcttg caagagcaag cttttagtat agacttaatc tactttataa atccaccctt
     4681 ctggtgcttc aacatcacca aactggatcc ctactaactc atcatatagt ttacgtgtcg
     4741 ctggaccaac ttctgtctca ctgtagaaga catggaagtc atctccgttt tgaattcctc
     4801 caattggtga aataactgcg gctgtaccac atgcgccagc ttctgtgaat ttatctaatt
     4861 cgtcgacaaa gacatctcct tcaatcgctt ccataccaaa cctttcttta gcaaggtaaa
     4921 gcaatgaata tttagtaatt gaaggtaaaa ttgaaggact caaaggggtg ataaactgat
     4981 tatctttagt aattccaaag aaattagctg ctccaacctc ttcaatttta gtatgtgtcg
     5041 ctggatcaag atagataaca tctgaaaatt gtttctcatg agcgtatttc ccaggaagaa
     5101 gactagcagc atagttacca ccaacttttg cagctcctgt accattcggt gctgctctat
     5161 catattcttt ggaaacaata aagttagttg gtgttaaacc acctttaaaa taactaccta
     5221 ctggcattgc aaataccgta aaaatatatt cttcagcagg tttaactcca ataatatctc
     5281 ccacaccaat taataaggga cgaatgtata atgttgcccc tgtaccatac ggtggaacaa
     5341 actcctcatt agcgcgtaca acagatttaa ccgctgcaat aaatttgtct gtcggaacat
     5401 gtggcattaa aagacgatct gctgttcttt gaagtcgttc tgcattttga tctggacgaa
     5461 ataactgaat agagccatct ttagttctat aagctttcaa tccttcaaaa gcttgttgcc
     5521 catagtggag ggcaggagaa ctttctgaaa tatgtagcgt tgcatcatct gtcaacttgc
     5581 catcatccca tttcccatct ttaaaatgcg aaatgtatcg gaatggtaat tttctgtaag
     5641 caaaacctaa attatcccaa tctaaattta ctgtcataag gtacctcttt ctttgggttt
     5701 ctaactattg tacgctatta tcattatttt ttcaatatta tttgtaaaat tcagaaaaat
     5761 taccaaacta gtaaaaacac aaatgtgttt tacttaattt taactgagaa tactccttcg
     5821 tcagaaatct tatcagatac aaaagagcca ttacttgtac gttcagaaaa actccattga
     5881 cttaaatcaa catcgtaaac ttttcctgtt ttagacataa tctcaagtac gaaagtatct
     5941 ccttcatgat tcgatactga actaaataaa tcaaattcaa aagatgaatc ctctaaatat
     6001 acaggtcctg ctttaaaaac gcgatgcggt ttggatttca attcacgaag aacttgaagt
     6061 cctcgattag cacgactagt tgtggggata acatcaatgg ccattcgttt aagacttcct
     6121 ctatgagtta ataaatataa acttgtggtg ttagctataa atgcagatac gatgtgatca
     6181 ctatctttta aattcatcgc ttttacacct gcggctttac taccaacgac tggtacatca
     6241 tttatactaa atctcaacgc ataaccatta taagtgacaa gtataacatc ctctaattgg
     6301 ataggagcaa cagtcacaac gtaatcatct acatttttta acttagcata tttagctgtt
     6361 tttgacttat atgtccgcca tggactgatt tcttgacgtt caaaacgctt tatttgccct
     6421 aagctagtga cagcaaaata agtttcttta gtaaaatcat ctactaattc agcataaata
     6481 atttcttcat ttgaagcaaa gttaacaaga ttttgtgata aatgttcacc aacgtctttc
     6541 catcgaatat cagctaattc atgaactgga cgataagcaa gattaccaag gttagtaaac
     6601 atcaataaat gttgggtagt ctttgcattg ctcacaaata tcaattcgtc atcttctcgc
     6661 ttaccaagtt catcaacagt tgatgcattg aaagagcgag gactggtgcg ttttacatat
     6721 ccgcctctag taacactgac gtatgtgtcc tcttcgataa ttaaacttgc agtatcgatt
     6781 tcaatagttt cagctaattc ttgaagttca gaacgtctag tattggcaaa tttctttttg
     6841 acctcacgta actcacgttt cattacgtta tacatcgtac gttcatcact aataattgcc
     6901 ttaagcattg tgatttgttg acgaagttct tcttcttctt ctctcaacgt cacaatatca
     6961 gtatttgtga gacgatataa ttgcaatgtg acgatagcct ctgcttgggc ttctgaaaac
     7021 tcatagctga ccttcaaatt ctctttcgca tcagctttat tctcagaagc acgaattaat
     7081 gcaatcactt catccaaaat agaaagaacc ctaatcagtc cctcaacaat atggagacgt
     7141 ttttcggctt tttctttatc aaattttgaa cgagcaataa taatttctct tcggtgagcg
     7201 atatagctag ttaatattct tgatagaccc acctgcttag gggtgtagtc atcaatagca
     7261 accatattga aattataatt cacttgtagg tctgtatatt taaaaagata atttaagaca
     7321 attgtttcat cggcttcttt tttgagctca atagcgatac gaagaccatc tctatcagac
     7381 tcatcacgca cctctgctat tcctggaact ttattattaa cacgaacatc atcgattcgt
     7441 tttactaaaa ctgacttatt aacttcataa ggaatttcag taacaataat ttgtttctta
     7501 ccacctttta aggtttcaat agcagttcgc gagcgaaccg ctactctccc cttaccagtc
     7561 tcatatgcct tacgaatttc atcttttcct tgaatgatag cgccagttgg aaaatctgga
     7621 ccaggtagaa attccattaa tttatctaat ttagctttag ggtgatcaat catgtacacg
     7681 acagcatcaa tgacttctgc taaattatgt ggtggaatat ctgttgcata gcctgcagat
     7741 attcctgttg ctccgttgac taaaagattg ggaaaggcag ctggtaaaac agttggttct
     7801 ttctctgtat catcgaaatt ccaggcaaag ggtacggtat tcttatcaat atcttgaaga
     7861 agataaccag caatttctga tagacgggct tccgtataac gcatagccgc cgcaggatct
     7921 ccatccatag aaccattatt tccgtgcatt tcaatcaatg tctcacgatt tttccaatct
     7981 tgagacatac gaaccatcgc gtcatagata gaggaatccc catgaggatg gaaattcccc
     8041 ataacattac cgactgattt tgcagatttg cgaaatccct tttcaaaagt attaccatct
     8101 ttattcattg aataaaggat tcgacgttga acaggtttta agccatcacg aatatccggc
     8161 aacgcccgtt cttgaatgat atacttagaa tagcgcccaa aacgctctcc cataatgtcc
     8221 tcaagggaca tattttgaat attagacata tttttaattt tctcctagtt ggagtggctt
     8281 agtattattt ttccacttat ttgaaatgaa tgattggctc tcttgtctaa cgcatacaat
     8341 attacttgta cactataaaa catgaataac caagactctt tattaaaata ctgtattctc
     8401 ttctaaagta aacttgacat tatcttcaat ccattggcgt cttggaggaa ctttatcacc
     8461 catcaagaca ttaactcgac gttctgctct tgctaaatct tcaattgtta cacgaattaa
     8521 tgtccttgtc tcagggttca tagtggtttc ccacaattga tcggcattca tttccccaag
     8581 ccctttatat cgttgtaata gtgagccttt accaaatttt tgtcgtaatt cttctaactc
     8641 tatatccgtc caagcatact caacaatttc tttcttacct tttccttttg acattttata
     8701 caaaggtggc aaagcaatat agacatgtcc ctcttcaaca agaggtctca tatagcgata
     8761 aaagaaagtt aataatagtg tctgaatatg agctccgtca gtatcagcat cggtcataat
     8821 gataacttta tcataattaa tatcgtcaat attaaaatca ggaccaacac ctgcaccaat
     8881 ggtatggatc attgtattaa tttcttcgtt ttttataata tcagccatct ttgccttagc
     8941 tgtattcaat acttttcctc gcaacggtaa aatagcttga aactttctat cacgaccttg
     9001 cttagcagag ccacctgcag agtctccttc gactaaatag agttcatttt tcttagcatt
     9061 ttttgattgt gcaggggtta attttccaga aagtagacct ttatcttttt tcgacttttt
     9121 cccatttcta ctttcatcac gagctttacg tgcagcttca cgcgcatcac gtgcttttat
     9181 tgccttacgg attaaattag atgctaaatc tccattttcc ataagaaagt aagttaactt
     9241 ttcagaaaca ataccatcaa caattggtcg tgctaaggga cttcctagtt tatctttagt
     9301 ttggccttca aattgcaagt gctcctcagg tacaagaata gataaaatgg ctgatagccc
     9361 ctcacgataa tcagaacctt caaggttttt atctttttct ttaagaagtc ctgttttacg
     9421 tgcatagtca ttcatagact ttgtaatagc tgatttcaag ccagtttcat gtgttccacc
     9481 atctttagta cgaacattat tgacaaaaga aagaatatta tctgaaaaac catcattata
     9541 ctgcaaagca acttcgatat gaaattcttg ttcttcacct tcgaaaaaca tgattggtgt
     9601 caacgtttct ttatcttcat ttaaatactc tacaaaatct tgaacaccgt tttcataatg
     9661 aaattctaga tgctctgctt cttctgaacg attatctgtt aaggttaaag taacattttt
     9721 taagagaaag gcagattcct ttaagcgctc tgcaattgta ttaaacttaa aatctatcgt
     9781 tgaaaatact gactgatctg gcataaagct gacagatgtc ccagattttg attttggtgc
     9841 cgtgccgatt tttttcaggg ttgtaacggg cttaccacca ttttcaaaac gttgtctgta
     9901 aatagctccg tcgcgaatga tttcaacttc taaccaagaa gacaaagcat taactacaga
     9961 tgatccaaca ccatggagac cacctgatgt tttataacca ccttgtccaa attttccacc
    10021 tgcatgaagc accgtgaaga tgacttcaac agtaggtttt cccatagcat gcattccagt
    10081 tggcataccg cgtccatggt ctgtaacagt tatactccca tctttgttaa taataacatc
    10141 aatgcgattt ccaaaacctg atagagcttc atcaactgca ttatctacaa tttcccacac
    10201 taaatgatgt aaaccagttc catctgtgga acctatatac atccctggac gcttacgaac
    10261 agcatctaat ccttctaaga cttgaatagc atcgtcgcca taattcgtca ctgttatatc
    10321 ttgtttagcc aaagtgacct ccaaattttt tcatcttttt tatattacaa gttttttgct
    10381 gtttttgcaa aattattttt ttaaaaatag caaaaagact tttatgctta tgttataata
    10441 agggtatgag tattataatt atgattatca ttgcctatct gttaggctct atccaaactg
    10501 gactttggat tggtaaatat ttttaccaag tcaatcttcg tcaacacggt agtggtaata
    10561 ctggcaccac gaataccttc cgtattttag gtgtaaaagc tggcattgtc actttgacta
    10621 ttgacatttt aaaagggact ctagcaacac ttattcctat catgttaggt gtaacgacag
    10681 tatctccatt ttttatcggt ttttttgcta ttataggtca cactttccct atttttgcac
    10741 aattcaaagg tgggaaagcc gttgctacaa gcgctggggt tttgctagga tttgcacctt
    10801 cttttttcct ttacttatta gttatctttt tattaacgct ttatcttttt agcatgattt
    10861 cgctatccag tattacagta gctgtcgtag gtatcctgag tgtcttaatt tttccattag
    10921 taggttttat attaacggac tacgactgga tatttaccac tgtggttatc ttaatggccc
    10981 tcacgattat tatccgacat caggataata tcaaacgtat tcgaaaaagg caagaaaatt
    11041 tggttccgtt tggcttaaac ctaagtaaac aaagaaataa ataataaaaa accgctagta
    11101 gcggtttttt attataagac taacaatgtt tcgagagtag atttcccttc taatttaacc
    11161 aatcaatctc atttattcct ttttcttcta agaaatgatt tgttctagaa aaaggtctgc
    11221 taccaaagaa accacgataa gcagataggg gactcggatg aggactctca ataatgtgat
    11281 gaataggatt atcaattagc gactttttct ttcgagcata gccaccccac agaataaaga
    11341 ctacgggtgt ttccttttgg ttaaccactt tgataacagc atcagtaaag ggttcccata
    11401 taagcccagc atgaccatta gcttggtgtt ctggaactgt taaacaagca tttaatagca
    11461 gcactccttg ttgcgcccat gatgttaagt catgatgtga acgtgacccg atatcttctg
    11521 ctaattcttt tagaatattt tgaagtgaag gaggagctgg taaattgtct ggaacagaaa
    11581 atgagagtcc ttgagcttgt tgaggtccat ggtaaggatc ttggccaata atcactactt
    11641 ttacgttttc tagtggtgtt atctgtatgg cattgaatac cttatctctt ggaggataaa
    11701 cgattccgct ttcgtaaact gcatccataa atgtattaat tttattgtag taatgattgg
    11761 gtagttctct ttttattaaa tcatgccaac tagaatgttt catttataac tccttacttt
    11821 tagatatctg tattactagt atagcaatat tttataaaaa acgttactgc tttattcatt
    11881 caaagcagta acgttttttt attaatcttc gtccttaaga cctagagcca aatcagataa
    11941 ggacactggt ctagaatttt cttctttcaa agaagtattt ataatgttta aagaagtata
    12001 aacatcaata aggattccaa atacatgtgc acgttcatca cctaaaattg cttgaaaaac
    12061 cgtctgtgat tgacagaatt ctaaaagatt atctaagccc ttaatccatt cttctacaat
    12121 agccagagcc ttattcatga ctttaaatga cgattcatca taccaataaa tagaacgaat
    12181 cagttttttt tgcttactca aagcttctat tttagaaagt atctcaccta gttcattctg
    12241 cacaatagca atagaaaaat gtttccccag taaagatggg gttttttcta ctaatataat
    12301 ttcatcacga aacaaggtta aataatgaat actgactaac aatttttgaa ttagcgcttg
    12361 gtgatcgtgt gtttcagttt ctctcatact caaccatcct tactatttct gactggtaaa
    12421 acta
//
LOCUS       DATRNK010000058        11932 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.058, whole genome shotgun sequence.
ACCESSION   DATRNK010000058 DATRNK010000000
VERSION     DATRNK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11932
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.058"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..162
                     /locus_tag="VBC52_001617"
     CDS             <1..162
                     /locus_tag="VBC52_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HEO6883186.1"
                     /translation="GEGKEQVSRSIFLGMKDKEIIRKLNQEYHIAFNTKTTPTGNDGA
                     VEVNILDYI"
     gene            198..1064
                     /locus_tag="VBC52_001618"
     CDS             198..1064
                     /locus_tag="VBC52_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO6883187.1"
                     /translation="MDISILQAVLIGLWTAFCFSGMLLGLYTNRCIVLSLGVGVILGD
                     IQTALAVGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITTAGTKGKITPEAALA
                     LSTPIAVGIQFLQTATYTAFAGAPETAKKALQAGNFRGFKIAANGTIWAFAGLGFGLG
                     VLGALSTQTLTDLFALIPPVLLNGLTLAGKMLPAIGFAMILSVMAKKELIPYILLGYV
                     LAVYFGLPVLTPTANGDGVLTSVATNSVLGVPTIGVAIIATIFALLDIFRKPAALTKE
                     TKTEGDNQDDWI"
     gene            1051..1866
                     /locus_tag="VBC52_001619"
     CDS             1051..1866
                     /locus_tag="VBC52_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6883188.1"
                     /translation="MTGSKKLAKSDYTKTALRAFYLQNGFNYSNYQGLGYANVIYPAL
                     KKYYGDDKKALAGALEENVEFYNTNPHFLPFVTSLHLAMLDNERPEEEIRGIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLATDGLVVGPILFFVAMNTILTGIKLVTGMYGYR
                     LGTSFIDKLSEQMSVISRAANIVGVTVISSLAATQVKLTIPYTFAPEKVTSTTQKIVT
                     VQGMLDKIAPALLPALYTFLMFYLIKNKKWTTYKLVILTVIIGILGSWLGILA"
     gene            1946..3850
                     /locus_tag="VBC52_001620"
     CDS             1946..3850
                     /locus_tag="VBC52_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEO6883189.1"
                     /translation="MKDRLYNKFKDFDREFCQKYIKTYQSNAYQEMKASVNLMMRNTF
                     VFNDNWDMEPCSKAYCLDPLEWDKPVTDDPEWLYMLNRQTYLFKFLVVYIVEGDKSYL
                     RQMKYFMYHWIDCQFTLKPEGAVSRTIDTGIRCMSWLKVLIFLDYFGLITETKKIKLL
                     TSLREQITYMRDYYREKDSLSNWGILQTTAILACLYYYEDELNLPEIQSFAEEELLLQ
                     IKLQILDDGSQYEQSIMYHVEVLKSLMELVILAPKYYLPLEETIEKMVTYLIAMTGPD
                     YCQLAIGDSDVTDTRDILTLATLVLKSSKTKSFSFDNVNLETLLLFGKPSIYLFEEIP
                     RATIGESAYLFPDSGHVCLRDDRRYIFFKNGPFGSAHTHSDNNSVCLYDKKKPIFIDA
                     GRYTYKEEQLRYDFKRSTSHSTCTLDEQPLEMIKDSWTYTSYPKCDYCQLTSKDRYHL
                     VEGQLHVQRASDIYYHKRWLLTLPQAITLVIDKVSCPGEHVLTNQYILDDQVIYENGL
                     VNDLKLVSPTTFNLEDCLISKRYNQLTESHKLVKKIKFVDEVMDYTLIVDRNCQVKCV
                     PLVQTNSHKELSNSIAFDIRSQDFHYLIGVLMDDIIFGDKLYLMQGIKCKGKVIVYDK
                     NNGKMSRLKN"
     gene            3929..4930
                     /locus_tag="VBC52_001621"
     CDS             3929..4930
                     /locus_tag="VBC52_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6883190.1"
                     /translation="MSKKMTINDIAQLSKTSKTTVSFFLNQKFEKMSDETRQRIQEVI
                     DETGYRPSTIARSLNSKKTKLLGVLIGDITNTFSNQIVKGIEHITKQKGYQIIVGNSN
                     YDAKSEEDYIENMLNLGVDGFIIQPTSNFRKYSRILKEKKKPMVFFDSQLYEHKTSWV
                     KANNYDAVYDMTQECLNRGYKKFIMITADTSLLSTRIERASGFMDALKDNGFGYDTLV
                     IEDDDHSKSDIEDFLKAVVPDKEETLVFAPNCWALPMVFTAMKNLNFDMPRVGLVGFD
                     NIEWTDFSSPKVSTIVQPAYEEGEQVAQILINRIEGDDSVDNQQIVDCQMFWKESTF"
     gene            complement(5001..5615)
                     /locus_tag="VBC52_001622"
     CDS             complement(5001..5615)
                     /locus_tag="VBC52_001622"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO6883191.1"
                     /translation="MSAIDKLVKASHLIDMNDIIREGNPTLRKVAEEVTFPLSEKEEI
                     LGEKMMQFLKHSQDPIMAEKLGLRGGVGLAAPQLDISKRIIAVLVPNVEDAQGNPPKE
                     AYSLQEVMYNPKVVSHSVQDAALSDGEGCLSVDREVPGYVVRHARVTIEYFDKTGEKH
                     RLKLKGYNSIVVQHEIDHIDGIMFYDRINEKNPFAVKEGLLILE"
     gene            complement(5681..6325)
                     /locus_tag="VBC52_001623"
     CDS             complement(5681..6325)
                     /locus_tag="VBC52_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEO6883192.1"
                     /translation="MDIENYCQDYCLETIFPKHYFKDLQVITLTAGQPVCKQGEQLEY
                     LHYIVKGRFKIVRRLFNGKEHILDIKTKPTLIGDIELLTNRQIVSSVIALEDLTVIQL
                     SLKGRKEKLLTDATFLLKLSQELAQAFHDQNIKASTNLGYTVKELLASHILAIEEQGY
                     FHLELSSLADSFGVSYRHLLRVIHDMVKEGLIQKEKPKYFIKNRFALESLNIQP"
     gene            6437..7600
                     /locus_tag="VBC52_001624"
     CDS             6437..7600
                     /locus_tag="VBC52_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6883193.1"
                     /translation="MKIFMEKLSLLSLSLILLSTFSTSPALPQMISYYRDKGLPSPQV
                     ELLFSIPSMAIIFILLITPWLSKKLSEKHMIIFGLLLTALGGGLPVVSQNYLLVFVSR
                     LLLGSGIGFINARAISVISEYYQGKERRKLLGLRGSFEVLGNAGLTALVGLLLTFGWS
                     KSFMIYFLALPILVLYLVFAPKKVVKGTNDKIKTKGQKVPKADLTYIVALAILAGFVI
                     TINTGINLRIPLLVVEFGLGTPAQASLVLSAMMLMGIIAGMSFGQLIAMFHKQLIPIC
                     LVLFSLTLLGVGLPSNLMVLTISAMASGFLYSLMVTAVFSLVADRVEYSLVGSATTLV
                     LVFCNIGGASAAILLSCFDHLLGQINAVFYVYAILSLAVGMIYFFKCLFFKKM"
     gene            complement(7646..8629)
                     /locus_tag="VBC52_001625"
     CDS             complement(7646..8629)
                     /locus_tag="VBC52_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6883194.1"
                     /translation="MIDKYYNWATLGTGVIANELAQALEARGQKLYSVANRTYDKGLE
                     FANKYGIQKVYDHIDQVFEDPKVDIIYISTPHNTHISFLRKALANGKHVLCEKSITLN
                     STELKEAIDLAETNHVVLAEAMTIFHMPIYRQLKTLVDSGKLGPLKMIQMNFGSYKEY
                     DMTNRFFSRDLAGGALLDIGVYALSCIRWFMSEAPHNITSQVTFAPTGVDEQVGILLT
                     NPANEMATVSLSLHAKQPKRATIAYDKGYIELFEYPRGQKAVITYTKDGHQDIIEAGK
                     TENALQYEVADMEEAVSGKTNHMYLNYTKDVMDIMTQLRQEWGFTYPEEEK"
     gene            8777..10672
                     /locus_tag="VBC52_001626"
     CDS             8777..10672
                     /locus_tag="VBC52_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HEO6883195.1"
                     /translation="MIKYQDDFYQAVNGEWAKTAVIPDDKPRTGGFSDLADDIEALML
                     STTDKWLADENKPSDTILNHFIAFHKMTADYQKREEVGVSPVLPLIEEYKGLQSFSEF
                     ASKVAEYELEGKPNEFPFGVAPDFMNAQLNVLWAEAPGIILPDTTYYSEDNEKGKELF
                     ALWRKSQEDLLPLFGLSEQEIKDILDKVLALDAKLAQYVLSREESSEYVKLYHPYNWE
                     DFTKLAPELPLDAIFQKILGQKPDKVIVPEERFWTEFASDYYSESNWELLKADLILSA
                     ANAYNAYLTDDIRIKSGVYSRALSGTPQAMDKKKAAYYLASGPYNQALGLWYAGEKFS
                     PEAKADVEHKIATMIDVYKSRLEKADWLAQSTREKAIVKLNVITPHIGYPEKLPETYT
                     KKIIDPKLSLVENATNLDKISIAYGWSKWNKPVDRSEWHMPAHMVNAYYDPQQNQIVF
                     PAAILQAPFYALEQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNNWWTDEDFE
                     AFKKLTDKVVEQFDGLESYGAKVNGKLTVSENVADLGGVACALEAAQRESDFSARDFF
                     INFATIWRMKARDEYMQMLASVDVHAPAQWRTNITVTNFEEFHKEFDVKDGDNMWRPV
                     EKRVIIW"
     gene            complement(10722..11675)
                     /locus_tag="VBC52_001627"
     CDS             complement(10722..11675)
                     /locus_tag="VBC52_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEO6883196.1"
                     /translation="MKKLTKGSHIRVLSPSSSIERLGGFEANLLAKEVLEKLGFQVSF
                     SKHYLENDILYSASIASRVEDLHEAFADPSVDAILATIGGFNSNELLPYLDYDLISKN
                     PKIICGYSDSTAFLNAIFAKAKIQTYMGPAYSSFKMKEGQPYQTQTWLTAMTENHYEL
                     WPSEEWSSDPWYDPSKPRQFFPTEWKIYNHGKASGTIIGGNLSTFGLLRGTPYAPKIE
                     RYVLLIEEAEESNFYEFDRNLAAILQAYPHPQAILMGRFPKECGMTPQVFEYILSKHA
                     IFKEIPVIYDMDFAHTQPLLTVTIGAEISVDTTTLSLSIKE"
BASE COUNT         3765 a         1900 c         2348 g         3919 t
ORIGIN      
        1 ggagaaggta aagagcaggt aagtcgctct atcttcttag ggatgaagga taaagagata
       61 attagaaaat taaaccaaga atatcacatt gcatttaata ctaaaacaac tccaacaggt
      121 aacgatggtg ctgtggaagt taatatttta gattatattt agttggacaa acttactatc
      181 atataaagga gaaaaatatg gatattagta ttcttcaggc tgtcttaatt ggactgtgga
      241 cggctttctg ttttagtggc atgttgcttg gcctctatac aaatcgttgt atcgttctct
      301 ctctaggcgt tggtgttatt ttaggagata ttcaaacagc tttggcagtt ggagcaatat
      361 ctgaactggc ttacatggga tttggtgtgg gagcgggagg gacagttcct ccaaacccaa
      421 tcggtcctgg gatttttgga actttgatgg ctattacaac agctggaact aaaggaaaaa
      481 tcacaccgga agctgctctt gcattatcaa ccccaattgc agttggtatt caatttcttc
      541 aaacagcaac ttatactgct tttgcagggg cgcctgaaac agctaaaaaa gctttgcaag
      601 caggtaattt ccgaggattt aaaattgcag caaatggtac aatttgggca ttcgctggat
      661 tgggatttgg tttaggagta ctgggtgctc tttcaacgca aactttgaca gatttatttg
      721 ctctcattcc ccctgttttg cttaatggtc taaccttagc tgggaaaatg cttcctgcaa
      781 ttggttttgc gatgattctc tctgtaatgg ctaaaaaaga actcattcct tatatcctac
      841 ttggatatgt tctagctgtt tattttggtt tgccagttct aactccaaca gcaaatggag
      901 acggagtttt aacatcagtg gctactaaca gtgtgcttgg ggttccaact atcggggtag
      961 ctataattgc aacaatattt gctttgcttg atattttcag aaaaccagct gcacttacta
     1021 aagaaactaa gacagaagga gacaaccaag atgactggat ctaaaaaatt agcaaaaagt
     1081 gattacacta aaacagcttt acgtgccttt tatttacaaa atggctttaa ttacagtaac
     1141 tatcaaggtc ttggttatgc taatgtcata tatccagctc tcaaaaaata ttatggagat
     1201 gacaaaaaag ctttagcggg agctctagaa gaaaatgtgg agttttacaa tacaaaccct
     1261 catttcttac cttttgtaac tagcttacat ttagcaatgc ttgataatga gcggccagaa
     1321 gaagaaattc gcggtattaa gatggcgctt atgggtcctc ttgcaggtat cggtgattct
     1381 ttatcacaat tttgcttagc acccttgttc tcaacgattg cagcctcttt agcgacagat
     1441 ggtttggtgg tgggaccgat tctgttcttt gtggctatga atactatttt aacgggaatt
     1501 aaattagtta ctggcatgta tggttatcgt cttgggacca gttttattga taaattgagt
     1561 gaacaaatgt cagttatctc acgggcagct aatatcgttg gtgtaacggt catctcaagt
     1621 ttagctgcaa cacaagttaa gcttacaatc ccttacactt ttgctccaga aaaagtaact
     1681 agtacaacgc aaaagattgt tactgtccaa ggtatgcttg ataaaattgc accagctcta
     1741 cttccagcac tctacacttt ccttatgttt taccttatta aaaataaaaa atggacaact
     1801 tataagttag ttatccttac agttattatt ggtatccttg gtagctggtt aggtatttta
     1861 gcttaataaa attttgataa ataaggatta tgacatatca atgacatagt cctttttata
     1921 tcggacgagg acgttttgta aaaaaatgaa agatcgctta tataataaat tcaaggattt
     1981 tgatagggaa ttttgtcaaa agtatattaa aacataccaa tctaatgcct atcaagaaat
     2041 gaaagcctct gttaatttga tgatgaggaa cacttttgta tttaatgata attgggatat
     2101 ggaaccttgt tctaaagcat attgtcttga tcctttggag tgggataagc cagtaacaga
     2161 tgatccagaa tggttgtata tgctaaatcg tcaaacttat ctttttaaat tcctggtggt
     2221 ctacattgtt gaaggcgata agtcttacct tagacaaatg aaatatttta tgtaccattg
     2281 gattgattgt caatttacac taaagccaga aggggcggta agtcgtacta ttgacactgg
     2341 gatacgttgc atgtcatggc tcaaagtttt gattttttta gattattttg gattaataac
     2401 agaaactaaa aaaattaaac tattaactag tttacgggag cagataactt atatgaggga
     2461 ctattatcgt gaaaaagata gtctaagtaa ctggggaatt ttacaaacaa cagcaatatt
     2521 ggcgtgttta tactattatg aagatgaatt aaatctacct gaaattcaga gttttgctga
     2581 agaagaatta ttacttcaaa tcaagcttca aattttagat gacgggagcc agtatgaaca
     2641 atcaattatg tatcatgtag aagtcttgaa gtccttaatg gaactagtta tccttgcgcc
     2701 taaatattat ctaccattag aagaaactat tgaaaaaatg gttacctatc taattgctat
     2761 gactggtccg gattattgtc aactagctat aggggacagt gatgtgactg atactcgtga
     2821 tattctaact ttggcaacgc ttgtgttgaa atcttctaaa acaaaatcat tttcttttga
     2881 taatgttaat ttagaaactt tgcttttgtt tgggaagcca tcaatttatc tttttgaaga
     2941 aataccacgt gcgacaatag gagagtctgc ttatcttttt ccagattctg gtcatgtgtg
     3001 tttacgtgat gataggcgtt atatattttt taagaacggt ccatttggta gcgctcatac
     3061 tcatagtgat aataatagtg tttgtctcta tgataaaaag aaacctattt tcattgatgc
     3121 aggaagatat acttacaaag aagaacaact aaggtatgat tttaaacgtt cgactagtca
     3181 ttcaacatgt acccttgatg agcaaccctt agaaatgatc aaggactctt ggacatatac
     3241 ttcctatcca aaatgtgact attgtcagtt gacttcaaag gataggtacc atttggtcga
     3301 aggacaacta catgtccaaa gagcttctga tatctattac cataagcgat ggttgttaac
     3361 tttaccgcag gccattacct tagttattga taaggtgagt tgtccaggag agcatgtctt
     3421 aacaaatcaa tatattttag atgatcaggt catttatgaa aatgggcttg ttaacgactt
     3481 gaaattagta agtcctacga cctttaatct agaagattgc cttatttcta agcggtataa
     3541 tcaattgaca gaaagtcata aattagttaa gaaaataaaa tttgttgatg aggtgatgga
     3601 ttataccttg atagttgatc ggaactgtca ggtgaaatgt gttcctttag tgcaaacgaa
     3661 cagtcataag gaactaagta atagcattgc atttgatatt aggtctcaag actttcatta
     3721 tttaattgga gtgcttatgg atgatattat ctttggggat aaactctatt tgatgcaagg
     3781 gataaaatgc aaaggaaaag tcattgtata tgataaaaat aatggcaaaa tgagtcgttt
     3841 aaaaaattaa gagggctatt attatgctat actagtacta tattaaccat aaaaaataaa
     3901 atcagtaaaa ttagaaagga ggtttgttat gtctaaaaaa atgaccatta atgacatcgc
     3961 tcaattatca aaaacttcca aaacaacggt ttcttttttc ttgaatcaaa aatttgaaaa
     4021 aatgtcggat gagacgaggc aacgtattca agaggtgatt gatgaaacag gttatagacc
     4081 aagtacgata gcaagaagtc taaattctaa aaaaacaaaa ctactaggtg tcttaattgg
     4141 agatataaca aacacatttt caaatcaaat tgtaaaagga attgagcata tcaccaaaca
     4201 aaaaggctat caaattattg tgggtaatag taattacgat gccaaaagtg aagaagatta
     4261 tattgaaaat atgttaaatt taggtgttga tggtttcatt attcagccta catcaaattt
     4321 tcgaaagtat tctcgtattt taaaagaaaa aaagaaacca atggtatttt ttgatagtca
     4381 actttatgaa cataagacga gttgggttaa ggctaataac tatgatgctg tttatgatat
     4441 gacacaggag tgtctaaatc gagggtataa aaagtttatt atgattactg ctgatacgag
     4501 cttgcttagt acacgtattg agagagcttc gggattcatg gatgctttaa aggacaatgg
     4561 tttcggttat gacactttgg tgattgaaga tgatgatcat tcaaaaagtg atatagaaga
     4621 ctttctaaaa gcggtagttc cagataagga ggagacatta gtatttgcac caaattgttg
     4681 ggctttacca atggttttta ctgccatgaa gaatctcaat tttgatatgc cacgagttgg
     4741 tttagttggg tttgacaata ttgaatggac agacttttca tctcctaaag tgtcaacaat
     4801 tgtccagccg gcttatgaag aaggcgaaca agtagcacag attttgatta atcgtattga
     4861 aggagatgac tccgtcgata accaacagat tgtagattgc cagatgtttt ggaaagagtc
     4921 gactttttaa aaaagatttc ttattttatt tgctattaaa aaataaaacc tagagaaaat
     4981 tatctctagg ttatttgtcc ttattctaaa ataagtaagc cttctttgac agcgaaagga
     5041 tttttctcat tgatacggtc gtaaaacata ataccatcaa tgtggtcaat ttcatgttga
     5101 acaacaatag agttgtaacc ttttaatttg agtcgatgtt tttcaccagt tttgtcgaaa
     5161 tattcaatag ttacacgcgc gtggcggacg acatagccag gtacttcacg atctactgat
     5221 aggcatccct ccccgtcaga taaagcagca tcttgaacag aatgcgatac aactttaggg
     5281 ttgtacatta cttcttggag gctataagct tctttaggtg gatttccttg agcatcctca
     5341 acgtttggaa caaggacggc aatgatgcgt tttgagatgt ctaattgtgg tgcggctaaa
     5401 ccaacaccac cacgtaagcc tagcttctca gccataattg gatcttgaga gtgttttaaa
     5461 aattgcatca ttttttctcc gagaatttct tctttctcag aaagtgggaa tgtgacttct
     5521 tcagccactt tgcgaagggt tggattgcct tcgcggataa tgtcattcat gtcaattaaa
     5581 tgactagctt ttactaattt atcaatagca gacatatttt ctcctaactg tattctctga
     5641 tttagtttag cataaaataa ttaaatgata aagctacgct tcatggctga atatttaatg
     5701 attctagtgc gaaacgattt ttgataaagt attttggttt ttctttttgg ataaggcctt
     5761 ctttgaccat atcgtggata actcttaata aatgacgata actgacgcca aacgaatcag
     5821 ctaaactact taactcaagg tgaaagtaac cttgttcttc aattgctaaa atatgacttg
     5881 caagtaactc tttgacggta taccctagat tagtagacgc tttgatgttt tgatcatgaa
     5941 aagcctgagc tagctcttga ctaagtttta ggaggaaagt tgcgtctgtt aacagtttct
     6001 ctttccgtcc tttaagtgat aattgaatga ctgtaagatc ctctaaggca ataacagatg
     6061 atacaatttg tctattagtc aaaagttcaa tatcaccaat cagtgttggt tttgttttaa
     6121 tatctaaaat atgttctttg ccattaaaga gtcgacgtac aattttaaaa cgtcccttga
     6181 cgatgtagtg caagtattct aattgttctc cctgtttaca gacgggttgg ccagcagtta
     6241 gggtgattac ttgtaaatcc ttgaaataat gttttggaaa gatagtctcc aaacagtagt
     6301 cttggcagta gttttctata tccatgtatt gactccctat ttgagatagt tttaggacat
     6361 atgtcatata tttctagttt aatattgatt atactttatt tatataaaaa aagacatcag
     6421 aaagaggtat tgtcacttga aaatctttat ggagaagcta agtttactct ctttatcact
     6481 tatcttgcta tcaacttttt cgacatcacc agccctacca caaatgatat cttattatag
     6541 agataagggc ttaccatcac cccaagttga attattattt tcaattcctt cgatggctat
     6601 tatcttcatc ttgcttatca caccttggtt aagtaaaaaa ctttctgaga aacatatgat
     6661 tatctttggg ctattgctaa cagcattagg gggaggattg ccggttgtca gtcagaacta
     6721 ccttttagta tttgtatcgc gtcttttatt ggggagtggt attggtttta ttaatgctag
     6781 ggccattagt gtgattagtg aatattatca aggcaaagaa agaagaaaac tcttaggatt
     6841 aagggggtct tttgaagttt taggaaatgc tggtttgaca gctttagttg ggctattgtt
     6901 gacttttggt tggtccaaat catttatgat ttatttttta gctttaccaa tcttagttct
     6961 atatcttgtt tttgcgccta agaaagtggt gaaaggtaca aatgataaga ttaaaactaa
     7021 aggtcaaaaa gtccctaaag ctgatttgac ttatattgtc gccttagcta ttttagcagg
     7081 atttgtcata actataaata ctgggattaa tcttaggata cctctgctag tagtagaatt
     7141 tggtttgggt acgccagctc aagctagtct tgttcttagt gcgatgatgc ttatgggaat
     7201 aatagcaggt atgagttttg gtcagttgat agctatgttt cataagcaat taattccaat
     7261 ctgcctcgtc ttattttctt taacattgct tggagttgga ttgccttcta atctaatggt
     7321 actaacaatt agtgcgatgg catcaggttt cctttacagc ctgatggtca ctgctgtgtt
     7381 tagcctagta gcagaccgtg ttgaatattc gttggttggt tctgcaacga cattagtttt
     7441 agtattttgt aatattggtg gagctagtgc ggctattctt ctgtcttgtt ttgatcattt
     7501 actaggacaa ataaatgcag ttttctatgt ttatgctatt ttgagtttag cagttggtat
     7561 gatttatttc tttaaatgcc tattttttaa gaaaatgtag tgtatattaa ctaaaaagag
     7621 cttgacaatc attgtcaagc tctcttcatt tttcttcttc tgggtaggta aatccccatt
     7681 cttgacgtag ctgtgtcatg atatccataa cgtctttggt atagtttaag tacatgtggt
     7741 tagtttttcc tgaaacggct tcttccatat cagctacctc atattggaga gcattttcag
     7801 ttttgccagc ttcgataata tcttgatgcc catccttagt ataagtaata actgcctttt
     7861 gtcctcgcgg atattcaaaa agttcaatgt agcctttatc gtaagcgata gttgctcgtt
     7921 taggttgttt tgcatgtaaa ctaaggctga cagtcgccat ctcatttgct ggattggtta
     7981 gtaggatacc aacttgttca tcaacccctg ttggtgcaaa tgtaacttga gaggtaatgt
     8041 tgtgaggtgc ttctgacata aaccagcgaa tacaagaaag tgcataaaca ccaatgtcca
     8101 gcaaagcacc tcctgctagg tcacgactga aaaaacggtt agtcatatca tattctttat
     8161 aacttccgaa attcatttga atcattttta acggtcctaa ttttccacta tcaactaatg
     8221 tttttaattg gcggtaaatt ggcatatgaa aaatagtcat ggcttcagct aagacaacat
     8281 ggttagtttc ggctaaatct atggcttctt taagttcagt gctgtttaaa gtaatagatt
     8341 tttcgcaaag aacgtgctta ccatttgcta aagcctttcg taaaaatgag atgtgagtat
     8401 tgtggggagt agagatataa atgatatcca ctttagggtc ttcaaatact tgatctatgt
     8461 gatcataaac tttttggata ccatatttgt tagcaaattc aagtcctttg tcgtaagttc
     8521 tattagctac agaatataat ttttgtccac gtgcttccag tgcttgggct aattcgttgg
     8581 caataacgcc ggttccaagc gttgcccaat tataatattt gtcaatcata acaacctcct
     8641 tgttggttta tagtatagca aaaataacaa aatgatattt agagaaagca ccaatatgag
     8701 agtattttta atagtcaaac tctgaatttt ctgttagact gtaagagatt acatatgaga
     8761 tattaaggag aaaataatga taaaatatca agatgatttt taccaagcgg ttaatgggga
     8821 gtgggctaag acagctgtta ttcctgatga caaaccaagg acaggtggtt tttctgatct
     8881 tgctgatgac attgaagcat taatgctgtc aacgacagat aagtggctgg ctgatgaaaa
     8941 taaaccaagt gatacgattt taaatcattt tattgctttt cataaaatga cagctgatta
     9001 tcaaaaacgt gaagaggtag gagtatcacc agtattacca ttaattgaag aatataaagg
     9061 cctacaatct ttctctgagt ttgcatcaaa agttgcagaa tatgaactag aaggtaaacc
     9121 aaatgaattt ccatttggtg tggctcctga ctttatgaat gctcaattga atgtactctg
     9181 ggctgaagcg cctgggatta ttttaccaga tacaacttat tatagtgaag ataatgaaaa
     9241 aggaaaggaa ctctttgctc tttggcgtaa aagtcaagaa gacttactgc ctttgtttgg
     9301 cctttcagaa caggaaatta aagatatctt ggataaggtt ttagcattgg atgcaaaatt
     9361 ggcacaatat gtcttatctc gcgaagagag ttcggaatat gttaaacttt accatcctta
     9421 taactgggaa gatttcacaa aattagcacc tgaacttcca ttagatgcga ttttccaaaa
     9481 gatattagga caaaaaccag ataaagttat tgttcctgaa gaacgtttct ggacagaatt
     9541 tgcatctgac tattactcag aatctaattg ggaattatta aaggcagatc ttattttatc
     9601 agctgcgaat gcctataatg cttacttaac tgatgatatt cgtatcaaat cgggagttta
     9661 cagtcgagct ttatcaggca caccacaagc aatggataag aaaaaagcag cttactacct
     9721 tgcttcagga ccttacaatc aagctctagg tttgtggtat gcaggggaaa aattctctcc
     9781 agaagctaaa gctgatgttg agcataagat tgcaacgatg attgatgtct ataagtcacg
     9841 cttagaaaag gcggattggc ttgcacagtc aacgcgagaa aaagctattg tgaaacttaa
     9901 tgttatcacg ccacacattg gataccctga gaaactacca gagacttata ctaaaaaaat
     9961 cattgatcct aaactatcgc ttgtcgaaaa tgctactaat ttagacaaaa tttcaatagc
    10021 ctatggttgg agtaaatgga ataaaccagt tgatagaagt gaatggcata tgcctgccca
    10081 catggtcaat gcgtattatg atccacaaca aaaccaaatt gtcttcccag cagccatttt
    10141 acaagcacca ttttatgctt tagagcaaag ttcatcagca aattatgggg gaattggtgc
    10201 agtgattgca catgaaatat cacacgcttt tgacactaat ggagcttcat ttgatgaaca
    10261 tggaagtttg aataattggt ggactgatga agattttgaa gctttcaaaa aacttacaga
    10321 taaagtagtt gagcagtttg atggactaga atcttatggc gcaaaagtca atggtaaact
    10381 aacagtatca gaaaatgttg cagatttagg tggagttgct tgtgctttag aagctgcgca
    10441 gcgtgagtca gatttttcag cgagggattt ctttattaac tttgcgacaa tttggcgtat
    10501 gaaggcacgt gatgagtata tgcaaatgtt agctagtgta gatgttcacg ccccagcaca
    10561 atggcgaact aatattacag taacgaattt tgaagaattc cataaagagt ttgatgttaa
    10621 agatggtgat aacatgtggc gtccagttga aaaacgtgtt attatttggt aactctaaat
    10681 gataaactcc cgttactcag atgagtagca ggagtatttt tttattcttt tatagataaa
    10741 gataatgtag tagtgtcaac gcttatctca gctccaatag tcactgtcaa aagaggttgg
    10801 gtatgcgcaa aatccatatc ataaataaca ggaatttcct tgaaaatagc atgttttgaa
    10861 aggatatatt caaagacttg tggagtcatg ccgcattctt taggaaagcg ccccataaga
    10921 atagcttgtg gatgaggata agcttgtaaa attgcagcaa ggttgcggtc aaattcataa
    10981 aaattacttt cctcagcctc ttcaatcaag aggacatagc gttcaatttt cggtgcataa
    11041 ggagtacctc gaagtaggcc aaacgtagag agattacctc cgataatcgt accggaagct
    11101 tttccgtgat tataaatttt ccattctgtt gggaaaaatt ggcgtggttt gctgggatca
    11161 taccagggat cactagacca ttcttcacta ggccaaagtt catagtggtt ttctgtcatg
    11221 gcagtaagcc aagtttgtgt ttggtaaggt tgaccttcct tcattttgaa agaagaataa
    11281 gcaggaccca tgtaagtttg gatttttgct ttagcaaaaa tagcattaag gaatgccgtt
    11341 gaatctgagt aaccacaaat tattttggga ttcttagaga ttaaatcata atctaaatat
    11401 ggtaaaagct cgttggaatt gaatccgcca atggttgcta atatggcatc aacagaaggg
    11461 tcggcaaatg cttcatgtaa atcttctacg cggctagcaa tcgaagcaga atagaggata
    11521 tcattttcta gatagtgctt agagaatgac acttgaaatc ccagcttttc tagtacttct
    11581 ttggctaata ggttagcctc aaaaccaccc agacgttcaa tggaagaaga gggactaaga
    11641 acacgaatgt ggctgccttt tgttaatttt ttcattggtt aacctcctat ttaaattgtt
    11701 gttagtctac cacatatgga gataatgagc aacgatattc ttaaattcta tcaatttaag
    11761 aaatgcgtgt tatactagag atagccgatg aaagaaaaaa tacttattat taataatttg
    11821 gttattaaat aggaggttat gatgtctcaa ttatctttgg aagaacgatt gaatcgacta
    11881 aaagataaat acctttcttc taaaaatcaa gaattttcaa atgttcactc aa
//
LOCUS       DATRNK010000059        11863 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.059, whole genome shotgun sequence.
ACCESSION   DATRNK010000059 DATRNK010000000
VERSION     DATRNK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11863
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.059"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..188
                     /locus_tag="VBC52_001628"
     CDS             <1..188
                     /locus_tag="VBC52_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEO6883197.1"
                     /translation="TIIASTNAANLTVSQFKKQLEKGFGKASHNYISLQQLPEDFTVN
                     DKDQQSNYLKVFTIKVK"
     gene            188..865
                     /locus_tag="VBC52_001629"
     CDS             188..865
                     /locus_tag="VBC52_001629"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEO6883198.1"
                     /translation="MKIVVPVMPRSLEEAQEIDLSKFDSVDIIEWRADALPKDDIINV
                     APAIFEKFAGHEIIFTLRTTREGGNIVLSDAEYVELIQKINSIYNPDYIDFEYFSHKE
                     VFQEMLEFPNLVLSYHNFQETPENIMEIFSELTALAPRVVKIAVMPKNEQDVLDVMNY
                     TRGFKTINPDQVYATVSMSKIGRISRFAGDVTGSSWTFAYLDSSIAPGQITISEMKRV
                     KALLDAD"
     gene            959..2026
                     /locus_tag="VBC52_001630"
     CDS             959..2026
                     /locus_tag="VBC52_001630"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEO6883199.1"
                     /translation="MQVEVDLPNHSYHIKIEEGCFSEAGDWVSHLWQKQMITIITDSN
                     VEILYGESLVNQLKKQGFTVHVFSFAAGEASKTLEVANRIYGFLAKHHMTRSDGIIAL
                     GGGVVGDLAAFVASTYMRGIHFLQIPTSLTAQVDSSIGGKTGVNTSFAKNMVGTFAQP
                     DGVLIDPVTLKTLGNRELVEGMGEVIKYGLIDDIKLWHILEEMDGSIDSILDNALAII
                     YHSCQVKRKHVLADQYDKGLRMHLNFGHTIGHAIEVHAGYGEIMHGEAVAIGMIQLSR
                     VAERKNLMPRGISQDIYNMCLKFGLPVHYAEWDKDVLFDILSHDKKASGQFIKIVILP
                     QLGSATVHQIPLEEMRDYLEK"
     gene            2027..3193
                     /gene="aroC"
                     /locus_tag="VBC52_001631"
     CDS             2027..3193
                     /gene="aroC"
                     /locus_tag="VBC52_001631"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEO6883200.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLKLSAKDINEDLKRRQGGYG
                     RGNRMKIETDQVIISSGVRHGKTLGSPITLTVTNKDHSKWLDIMSVEDIEERLKQKRR
                     IKHPRPGHADLVGGIKYRFDDLRNALERSSARETTMRVAIGAIAKRILKEIGIEIANH
                     IVVFGGKEITVPDKLTVQQIKVLSSQSQVAIVNPSFEQEIKDYIDSVKKAGDTIGGVI
                     ETIVGGVPVGLGSYVHWDRKLDAKIAQAVVSINAFKGVEFGLGFKSGFLKGSQVMDSI
                     SWTKDQGYIRQSNNLGGFEGGMTNGEPIVVRGVMKPIPTLYKPLMSVDIDTHEPYRAT
                     VERSDPTALPAAGVVMEAVVATVLVTEVLEKFSSDNMYELKEAVKLYRNYVNHF"
     gene            3277..3612
                     /locus_tag="VBC52_001632"
     CDS             3277..3612
                     /locus_tag="VBC52_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEO6883201.1"
                     /translation="MANVYDLANELERAVRALPEYQAVLTAKAAIESDADAQVLWQDF
                     LATQSKVQEMMQSGQMPSQEEQDEMSKLGEKIESNDLLKVYFDQQQRLSVYMSDIEKI
                     VFAPMQDLM"
     gene            3787..5136
                     /gene="gorA"
                     /locus_tag="VBC52_001633"
     CDS             3787..5136
                     /gene="gorA"
                     /locus_tag="VBC52_001633"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEO6883202.1"
                     /translation="MSKQYDYIVIGGGSAGSGTANRAAMYGAKVLLIEGGQVGGTCVN
                     LGCVPKKIMWYGAQVSETLHKYSSGYGFEANNLSFNFTTLKANRDAYVQRSRQSYAAN
                     FERNGVEKIDGFARFIDNHTIEVNGQQYKAPHITIATGGHPLYPDIIGSELGETSDDF
                     FGWETLPNSILIVGAGYIAAELAGVVNELGVETHLAFRKDHILRGFDDMVTSEVMAEM
                     EKSGISLHAKHVPKSLKRDEGGKLIFEAENGKTLVVDRVIWAIGRGPNVDMGLENTDI
                     VLNDKGYIKTDEFENTSVDGVYAIGDVNGKIALTPVAIAAGRRLSERLFNHKDNEKLD
                     YHNVPSVIFTHPVIGTVGLSEAAAIEQFGEDNIKVYTSTFTSMYTAVTTNRQAVKMKL
                     VTLGKEEKVIGLHGVGYGIDEMIQGFSVAIKMGATKADFDDTVAIHPTGSEEFVTMR"
     gene            complement(5180..5632)
                     /locus_tag="VBC52_001634"
     CDS             complement(5180..5632)
                     /locus_tag="VBC52_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883203.1"
                     /translation="MSKGYSRQQKKSTKTNTDSTQSPAKHIKTGFSALQKTIALVGSV
                     LSIIVASITITNAINNHKDSSKTDSSTSKTVIIRESGQNSSNQVSTEKDETRASSSQT
                     LENSSASSTPSSSSNSAANNDQTSSSTNNDQTNNTTTDTSTIGNTTTP"
     gene            5879..7024
                     /locus_tag="VBC52_001635"
     CDS             5879..7024
                     /locus_tag="VBC52_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6883204.1"
                     /translation="MIYFDNSATTIPYPEVLRTYQEVASKIYGNPSSLHELGTTSSRI
                     LEASRKQIASLLELKVNEIFFTSGGTEADNWVIKGLAFEKQHFGNHIIVSDIEHPAVK
                     ESAKWLGEYGFEIDYAPVDDKGFVDVEALVKLIKPETILISIMAINNEIGSIQPIKAI
                     SDLLSDKPTISFHVDAVQAIGKIPTKDYLTERVDFASFSSHKFHGVRGVGFLYIKEGK
                     RISPLLTGGGQESDLRSTTENVAGIAATAKALRMVMDKEVVAIPKISKMKTIIHDELA
                     KYEDITLFSGKEDFSPNIITFGIKGVRGEVLVHAFEGHDIFISTTSACSSKAGKPAGT
                     LIAMGISTKLAQTAVRISLDDDNDMGQVEQFLTIFKQVYEKTKKVRG"
     gene            7026..8240
                     /gene="thiI"
                     /locus_tag="VBC52_001636"
     CDS             7026..8240
                     /gene="thiI"
                     /locus_tag="VBC52_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEO6883205.1"
                     /translation="MQYSEIMIRYGELSTKKKNRMRFINKLKNNMEHVLSIYPDVSVK
                     TDRDRGHVYLNGTDYHEVAESLKEIFGIQAFSPSFKVEKNVDTLVKAVQEIMTSVYKD
                     GMTFKITAKRSDHSFELDSRALNHTLGDAVFSVLPNIKAQMKQPDINLKVEIRDEAAY
                     ISYENIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAHDLTRKLTKFGGNIQFIEVPFTEIQEEIKEKAPEAYLMTLTRRFMMRITDRIR
                     ENRNGLVIINGESLGQVASQTLESMQAINAVTATPIIRPVVTMDKLEIIDIAQKIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNTEQYEKRMDVEGLVERAVAGIMVTTIQPQAD
                     SDDVDDLIDDLL"
     gene            8342..9520
                     /locus_tag="VBC52_001637"
     CDS             8342..9520
                     /locus_tag="VBC52_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEO6883206.1"
                     /translation="MARIPYQKTLIFCVAVIIAIFILGLSRDLAQSKGQKVANNNTVK
                     TARVVANGDILLHDVLYASARQPDGTYNFTPYFKEVKSWIESADLAIGDYEGTISSEY
                     PLAGYPLFNAPNEIATTMKETGYDVVDLAHNHILDSQLAGAINTVKTFNRLGLDTIGV
                     YLKDRNKEDILIKHVNGIKIAILGYSYGYNGMEANVSKSDYEKHMSDLDTKKIKQDIK
                     KAEKEADITIVMPQMGIEYQKKPTTEQVMLYHSMIKWGADIIFGGHPHVVEPSEVIKK
                     DGQKKFIIYSMGNFISNQRLETVDDIWTERGLLMDVTIEKKGQKTVIKKVKAHPTLVE
                     AKANGRYVEEGFPAYDYRTLVLEDYIEGGKKRSTIDQTMKDKVDKAYKEMNELVNLKW
                     "
     misc_feature    9596..9675
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            9710..10024
                     /gene="rplU"
                     /locus_tag="VBC52_001638"
     CDS             9710..10024
                     /gene="rplU"
                     /locus_tag="VBC52_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEO6883207.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKLDVEAGAEVTFNEVVLVG
                     GETTKVGTPVVEGATVVGTVEKQGKQKKVVSYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            10031..10369
                     /locus_tag="VBC52_001639"
     CDS             10031..10369
                     /locus_tag="VBC52_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEO6883208.1"
                     /translation="MIKATFTRNQSGYLYSAEISGHAGSGEYGFDVICAAVSTLSINF
                     INSLEALTTCQAQLIINDVEGGYMKIDLSSIPQHKEDKVQLLFESYLLGMTNLSKDSS
                     EFVSTIVMTH"
     gene            10391..10684
                     /gene="rpmA"
                     /locus_tag="VBC52_001640"
     CDS             10391..10684
                     /gene="rpmA"
                     /locus_tag="VBC52_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEO6883209.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGANVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            10900..11805
                     /locus_tag="VBC52_001641"
     CDS             10900..11805
                     /locus_tag="VBC52_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6883210.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAAKLFVSQPSLSVAVRDLETELG
                     FQIFTRTTTGAVLTNQGMTFYENALEVVKSFDSFEKQFSQSEATEQEFSIASQHYDFL
                     PPLITAFSKCNDNFSYFRIFESTTIRILDEVAQGNSEIGIIYINSQNKKGLLQRLDKL
                     GLEFVELIPFKTHIYLGKDHPLASKTSLIMTDLEGLPTVRFTQDRDDYRYYSENFVEV
                     LDSSVTYNVTDRATLNGILERTQAYATGSGFLDSRSVNGITVIPLEDHLDNQMIYIKR
                     KDRNLSQMALKFVAVMEEYFKGFNS"
BASE COUNT         3905 a         1793 c         2480 g         3685 t
ORIGIN      
        1 gaactatcat tgcatcaact aacgcagcta atctaaccgt atcacaattt aagaaacaat
       61 tggaaaaggg gtttggtaaa gcttcacata attacattag cttacagcag ttgcctgaag
      121 atttcactgt aaatgataag gaccaacaaa gtaattattt aaaagtattt acaataaagg
      181 taaaataatg aaaatagtag taccagtaat gcctcgcagt cttgaagagg ctcaagaaat
      241 agatttatca aaatttgata gtgttgatat tattgaatgg cgagctgatg ccttaccaaa
      301 ggatgacatt attaatgtag ctccagctat ttttgagaaa ttcgcaggtc atgaaattat
      361 ttttactctt cgtacaacgc gtgaaggtgg taatattgtc ttatctgatg ctgagtatgt
      421 tgagctaatc cagaaaatta attctatcta caatccagat tatattgatt ttgagtattt
      481 ttcacataaa gaagtttttc aagaaatgct agaatttcca aatttagtcc tgtcttatca
      541 caattttcaa gagacaccgg agaatattat ggagatattt tcagaattaa cagccctagc
      601 accacgagtt gtgaaaatcg cagtaatgcc aaagaatgaa caagatgtct tagacgttat
      661 gaattacact cgcggtttca agactattaa tcctgatcaa gtttatgcga cggtatctat
      721 gagtaaaatt ggacgtattt ctcgttttgc tggtgatgta actggatcta gttggacatt
      781 tgcatattta gattcatcta tcgcacccgg acaaattact atttcagaga tgaagcgtgt
      841 caaagcattg cttgacgctg actgatacga gataacaaaa tgttaaatat actgatgaga
      901 tccctagcaa ttgcttaggg tttttgtctt attaaaacaa acatgagagg acattagaat
      961 gcaggttgaa gttgacttac cgaatcactc ttatcatatc aaaattgaag aaggatgttt
     1021 ttctgaggct ggtgactggg tatcacattt atggcagaaa caaatgataa ctattatcac
     1081 agatagtaac gtagaaatat tatacgggga gtcgcttgtc aaccagttaa aaaaacaagg
     1141 ttttacagta catgttttta gttttgctgc gggtgaagct agtaaaactt tggaagttgc
     1201 taatcgtatt tatggttttt tagcaaagca tcatatgacc agaagcgatg ggatcattgc
     1261 acttggtgga ggcgttgtcg gagatttagc agcgtttgtt gcttctactt atatgcgggg
     1321 aatccatttt cttcaaattc caactagttt aacagcacag gttgattctt ccattggcgg
     1381 aaaaacagga gtcaacactt cattcgctaa aaatatggta ggtacttttg cacaacctga
     1441 tggtgtactg atagatcctg ttactctaaa aacattgggc aatcgtgaat tagttgaagg
     1501 tatgggagaa gtcatcaaat atggcttgat tgatgatata aaactctggc atatcttaga
     1561 agagatggat gggtctattg atagtatcct tgataatgca ttagctatta tttatcactc
     1621 ttgtcaagtt aaacgaaagc atgttttagc tgatcaatac gataaagggt taaggatgca
     1681 tttaaatttt ggccacacta tcggtcatgc tatcgaagta catgctggct atggagaaat
     1741 aatgcatgga gaagctgttg caattggaat gatccagctt tcccgtgtgg ctgagcgtaa
     1801 aaatcttatg ccaagaggta tcagtcaaga tatttataat atgtgtttaa aatttggcct
     1861 tccggtccac tatgcggaat gggataagga cgtcttattt gacatattga gtcatgataa
     1921 aaaagcaagt ggacaattta ttaaaattgt tatactgcct caattaggta gtgcaacagt
     1981 ccaccaaata cctttagaag aaatgagaga ttatttggag aaatagatga gatatttaac
     2041 tgctggtgag tcacatggac cgagtttaac agctattatc gaagggattc ctgcgggact
     2101 gaaattatct gctaaagata ttaatgaaga tttaaaacgc cgccaaggtg gttatggtcg
     2161 tgggaatcga atgaaaattg aaactgatca agtgatcatt agttctggtg tgcgccatgg
     2221 aaaaaccttg ggttcaccta ttacactgac agtaactaat aaggatcata gcaaatggct
     2281 ggacatcatg tcagtcgaag acattgaaga acgtctaaag caaaaacgtc gtattaagca
     2341 tcctagacct gggcacgcag atttagtagg tggaatcaaa taccgttttg atgatttgag
     2401 aaatgcttta gagcgttctt cggctcgtga gaccacaatg cgagtagcta ttggtgcgat
     2461 tgctaaacgt atcttgaaag aaattggtat cgaaattgct aaccatattg tagttttcgg
     2521 tggcaaagag attactgtcc ctgataagct aacagttcaa caaatcaaag tgttatctag
     2581 tcaatcgcaa gtagctatcg tcaatccatc atttgaacaa gaaattaagg attatattga
     2641 cagtgtgaaa aaggctggag atactatcgg aggagtgatt gaaactatcg ttggaggagt
     2701 tcccgttggt ttaggctctt atgtacactg ggatagaaag ttagatgcta agatagcgca
     2761 agcagttgta tctatcaatg cttttaaagg tgttgaattt ggtctaggtt ttaagtcagg
     2821 ttttttaaaa ggaagtcaag taatggactc catatcatgg acaaaggacc aaggttatat
     2881 acgtcagtca aataatttag gcggttttga aggcggtatg actaatggag agcctattgt
     2941 cgttagggga gtaatgaagc caattcctac gctttacaag ccactaatgt cagttgatat
     3001 tgatactcac gagccttata gagctacagt ggaacgttct gatcccacgg cacttccggc
     3061 tgcaggtgtc gttatggaag ctgtagtagc caccgtcctg gtgacagaag tgctagaaaa
     3121 attttcttca gacaacatgt atgaactaaa agaagctgta aaactatatc gcaattatgt
     3181 taatcatttt taagtgaaaa ctagtagcat tcaattgcaa aaaaaggtat aaaagtctac
     3241 aatagtggta agttcaagta gaaaaaggag taacttatgg ctaatgtata tgatttagca
     3301 aatgaattag aacgtgctgt tcgtgcttta ccagaatacc aagcagtttt aactgcaaaa
     3361 gcagctattg aaagtgatgc ggatgcacaa gtgctttggc aagacttttt ggctacccaa
     3421 tcaaaagttc aagaaatgat gcaatctggc caaatgccaa gtcaagaaga acaagatgaa
     3481 atgtctaaac ttggggaaaa aattgaatcc aatgaccttt taaaagttta ttttgaccaa
     3541 caacaacggt tgtctgtcta tatgtctgat atcgaaaaaa ttgtctttgc acccatgcag
     3601 gacttaatgt aatagtataa aagagagagc tccttgagtt ttctcttttg taatatttca
     3661 atttatgata gtctactgaa ctagcattgc ctacattata ttggttttgc attctacgta
     3721 gcagaaaatt gtgactgtaa acactttgtg atagagtata ggtatcaatg attcaaggag
     3781 agagtaatgt caaaacaata tgattatatc gttattggtg gaggtagtgc aggcagtggt
     3841 accgctaata gggcagccat gtatggagca aaagtcctgt taattgaagg tggacaagta
     3901 ggtggaactt gtgttaactt aggttgtgta ccgaagaaaa tcatgtggta tggtgcacaa
     3961 gtttctgaga cactccataa gtatagttca ggttatggtt ttgaagccaa taatcttagt
     4021 tttaatttta ctactctaaa agctaatcgc gatgcttacg tgcagcggtc tagacagtcg
     4081 tatgccgcta attttgagcg taatggggtc gaaaagattg atggatttgc tcgttttatt
     4141 gataaccata ctattgaagt gaatggtcag caatataaag ctcctcacat tactattgca
     4201 acaggtggac accctcttta ccctgatatt attggaagtg aacttggtga gacttctgat
     4261 gatttttttg gatgggagac cttaccaaat tctatattga ttgttggggc gggctatatc
     4321 gcggcggaac ttgctggagt ggttaatgaa ttaggcgttg aaacccatct tgcatttaga
     4381 aaagaccata ttctacgcgg atttgatgac atggtaacaa gtgaggttat ggctgaaatg
     4441 gagaaatcag gtatctcttt gcatgctaaa catgtaccta aatctcttaa acgcgatgaa
     4501 ggtggcaagt tgatttttga agctgaaaat gggaaaacgc ttgtcgttga tcgtgtaata
     4561 tgggctatcg gccgtggacc aaatgtagac atgggacttg aaaataccga tattgtttta
     4621 aatgataaag gttatatcaa aacagatgaa tttgagaata cttctgtaga tggcgtgtat
     4681 gctattggag atgttaatgg gaaaattgcc ttgacaccgg tagcaattgc agcaggtcgt
     4741 cgcttatcag aaagactttt taatcataaa gataacgaaa aattagatta ccataacgta
     4801 ccttcagtta tttttactca ccctgtaatt gggacggtag gactttcaga agcagcagct
     4861 atcgagcaat ttggagaaga taatatcaaa gtctatacat caacttttac ctctatgtat
     4921 acggctgtta ccactaatcg ccaagcagtt aagatgaagc tcgtaaccct aggaaaagag
     4981 gaaaaagtta ttgggcttca tggtgttggt tatggtattg atgaaatgat tcaaggtttt
     5041 tcagttgcta tcaaaatggg ggctactaaa gcagactttg atgataccgt tgctattcac
     5101 ccaactggat ctgaggaatt tgttacaatg cgctaatgct atcaaagaat acaaaaaaag
     5161 ctagggaacc tagctttttt taaggtgttg ttgtatttcc aatagttgag gtatcggtag
     5221 tagtattatt tgtttgatca ttgttagtac tagatgatgt ttgatcatta ttggcagcag
     5281 aatttgaact gctactcgga gtactactag cagaggaatt ttctagtgtt tggctggatg
     5341 atgcccttgt ctcatctttt tcagtagaaa cttggttact agaattttgt ccactttctc
     5401 ttataatgac ggtttttgaa gtagaagaat cagtcttact agagtctttg tgattattaa
     5461 tcgcattagt aatagtaata gaggcaacaa taatacttaa aacactaccg actaatgcaa
     5521 ttgttttttg aagtgcagaa aaacctgttt ttatatgctt agcgggtgat tgagttgaat
     5581 cagtattagt cttagtagac tttttctgtt gtcttgaata tccttttgac atgacgccct
     5641 ccttaaatgt acatcaacta ttatatgatt ttatcctaaa aaaagctaaa gaaaaaataa
     5701 ttttaaacat ttaaataagc aacctaggtt attaagctat gttagtgtaa tgggtagtga
     5761 gttttgtgat aaattatgag aattatcata aatattattt tgggatgttc acaaactaat
     5821 ataaatagta gggaaattat gctataataa cactgattga ctttagaagg acagatttat
     5881 gatttatttt gataattcgg caactactat tccctatcca gaagttttaa ggacttatca
     5941 agaagtagct agtaaaatct atggaaaccc ctcaagttta catgagctag gaacaacatc
     6001 ttctcggatt ttagaggcta gccgtaaaca aattgctagt ttacttgagc ttaaagtaaa
     6061 tgaaattttc tttacatctg gcggtacaga ggccgataat tgggttatta agggccttgc
     6121 gtttgagaaa caacattttg gaaatcatat tattgtttct gatatcgaac acccagctgt
     6181 taaagagtct gctaaatggc ttggagagta tgggtttgag attgattatg cgccagtaga
     6241 tgataaagga tttgtagacg tagaagcact ggtcaaatta attaaaccag aaacgattct
     6301 tatttcaatt atggcaataa acaatgaaat tggttctatt cagccaataa aagctatttc
     6361 agacttgttg tcagataaac ctactatatc gtttcatgtt gatgctgttc aagcaatagg
     6421 taagataccg actaaagatt atttgactga gcgagttgat tttgcatctt tttctagtca
     6481 taaatttcac ggcgttcgtg gggttggctt tctatatatt aaagaaggaa aacgtatttc
     6541 accattacta acaggtggtg gacaagaatc tgatttacgt tcaactactg agaatgtcgc
     6601 agggattgca gctactgcta aagctttacg aatggttatg gacaaggaag tagtagctat
     6661 tcctaaaata agtaaaatga aaaccattat tcatgatgaa ttagccaaat atgaggatat
     6721 tacgttattt tcgggtaagg aagatttttc acctaacatt atcacgtttg gtatcaaggg
     6781 tgtccgagga gaagttcttg tccatgcctt tgagggacat gatattttta tctcgacaac
     6841 tagcgcttgc tcttctaaag caggtaaacc agcaggaact cttatagcaa tgggaatttc
     6901 aactaagtta gcccaaactg ctgtacgtat cagcttagat gatgacaatg atatgggaca
     6961 agtagagcaa tttttaacta tttttaaaca agtttatgaa aaaaccaaga aagttagagg
     7021 ataagatgca gtattcagaa attatgattc gttatggaga gctctctact aagaagaaaa
     7081 accgtatgcg cttcatcaat aagttaaaaa ataatatgga gcatgtactc tccatttatc
     7141 cagatgtttc agtaaaaaca gatcgtgata gaggacatgt atatctcaat ggtacagatt
     7201 atcatgaagt tgcagagtcc ttaaaagaga tttttggtat ccaagctttt tctccatctt
     7261 ttaaagtaga aaaaaatgtt gatacattgg taaaagctgt ccaggaaatt atgacttccg
     7321 tttataaaga tgggatgact tttaaaatta ccgcaaaacg tagtgaccac tcatttgaat
     7381 tggatagccg tgctctaaat catactttag gagatgccgt tttttcagtc ttgccaaata
     7441 ttaaggctca gatgaagcaa ccagatatca atcttaaagt cgagatacga gatgaggctg
     7501 cttatatttc atatgagaat attaggggtg caggaggatt accggtagga acatctggaa
     7561 aagggatgct gatgttgtct ggtgggattg attctccggt ggcaggttac ctagcgttaa
     7621 aacgtggtgt agatatagaa gcagtccatt ttgcaagtcc tccttatact agcccaggtg
     7681 cattgaaaaa agcacatgat ttaacacgta aattgacaaa atttggtggt aatattcaat
     7741 ttattgaagt tccattcaca gaaattcaag aggaaattaa ggaaaaagct cctgaagctt
     7801 acttgatgac gttaacacgt aggtttatga tgcgtattac agatcgtatt cgtgagaacc
     7861 gaaatggtct tgttattatt aacggtgaaa gtttagggca ggtggcaagc caaacgctag
     7921 aaagtatgca agccattaat gctgtcactg caacaccgat tattcgtcct gtggtcacga
     7981 tggataagct agaaattatt gatattgctc aaaaaataga tacttttgat atttcaattc
     8041 aaccatttga ggattgctgt acgatttttg caccagatcg cccaaaaact aaccctaaaa
     8101 ttaagaatac agaacagtat gagaaacgta tggatgtaga aggtctggta gagagagcag
     8161 ttgcagggat tatggtaact actattcaac ctcaagcaga tagtgatgat gttgatgact
     8221 tgattgacga tttattataa tgaattttta aactacagat ttattctgta gttttttatt
     8281 aaaatgattg cgctgtcata aggttataaa aaatgctata atacgaataa aggaggcaag
     8341 tatggcaaga atcccttatc agaaaacact tattttttgt gtagccgtta ttattgctat
     8401 ttttattttg ggattatcta gggatttagc acaatctaaa gggcaaaagg ttgcaaataa
     8461 caatactgta aaaacagcac gtgtggttgc aaacggagat attttactac acgatgtact
     8521 atatgccagt gcgcgccaac ccgacggcac ttataatttt actccttatt ttaaagaggt
     8581 aaaatcttgg attgagagcg ctgatttagc gattggtgat tatgaaggga cgattagttc
     8641 agaatatcct ttagcgggct atcctttatt taatgctcct aatgagattg caactactat
     8701 gaaagaaact ggatatgatg tcgttgactt agcacataat catattctgg attcacaact
     8761 ggcaggtgct atcaatactg tcaagacttt taatcgtctg ggattggata ctattggggt
     8821 ttatttaaag gatagaaata aagaagatat cttaattaag catgtaaatg gtattaaaat
     8881 tgctatttta ggttattcgt atggctacaa tggtatggaa gctaatgttt caaagagtga
     8941 ctatgaaaaa catatgtctg atttagatac taaaaagatt aagcaagata ttaaaaaggc
     9001 tgaaaaagaa gcagatatca ccattgtcat gccacagatg gggatagaat atcaaaaaaa
     9061 acctactaca gaacaagtaa tgctttatca cagtatgatt aaatgggggg cagacattat
     9121 attcggagga caccctcacg ttgttgaacc gtctgaagtg ataaaaaaag atggccagaa
     9181 aaaatttatt atttactcta tgggaaattt tatttcaaat cagcgtttag aaacagttga
     9241 tgatatctgg acagaaagag gattattaat ggatgtaaca atagagaaaa aaggtcaaaa
     9301 aacagtaatt aaaaaagtta aggcgcatcc aacactagta gaagcaaaag caaatggccg
     9361 ttatgttgaa gaaggctttc cagcttatga ttataggacg ctagttttag aggattatat
     9421 tgaaggtggt aagaaacgtt ctacgattga tcagactatg aaagataaag ttgataaagc
     9481 atataaggag atgaatgagt tagttaattt gaagtggtaa agttgataat tttattttgt
     9541 tttatcttgc ataaaaaaag agtttatgat aaaatttgaa tgttgactaa atgcacatcc
     9601 tgtgcaaccg cgcgaaaatc gttagattaa gtagggcaag gctctcacga tttataggcg
     9661 agtcttcaca gagggaaacc tcacaagaaa tttttatagg aggtgcatca tgagcacata
     9721 cgcaatcatc aaaactggtg gtaaacaagt taaagttgaa gtaggacaag ctatctacgt
     9781 tgaaaaactt gacgttgaag caggagcaga agttacattt aacgaagttg ttcttgttgg
     9841 tggtgaaaca actaaagttg gtactccagt tgttgaagga gctactgttg ttggtactgt
     9901 tgaaaaacaa ggcaaacaaa agaaagttgt ttcttacaaa tacaaaccta aaaaaggtag
     9961 ccaccgtaaa caaggtcacc gtcaacctta tacaaaagtt gttatcaacg caatcaacgc
    10021 ttaatttttc atgattaaag caacttttac tcgtaatcaa tcaggctatc tttatagtgc
    10081 tgaaatcagc ggtcatgctg gtagtggcga gtacggcttt gatgtgattt gtgcagctgt
    10141 tagtacttta tcaattaatt ttattaattc attagaggct ttaacgacct gccaagcgca
    10201 attgattatc aatgacgttg aaggtggtta tatgaagata gatttatctt ctataccaca
    10261 acataaagag gataaggttc aattattatt tgagtcttac cttcttggaa tgactaatct
    10321 gtctaaagat tcttctgaat ttgttagtac aatagtcatg acacactaat ttaagagagg
    10381 acacaacatt atgttaaaaa tgaatcttgc taacttgcaa cttttcgccc acaaaaaagg
    10441 tggaggttct acatcaaacg gacgtgattc acaagcaaaa cgtcttggag ctaaagcagc
    10501 tgacggacaa acagtatcag gtggatcaat tctttaccgt caacgcggta cacacatcta
    10561 tccaggtgct aacgttggac gtggtgggga cgatacactt tttgctaaag ttgaaggtgt
    10621 tgttcgtttc gaacgtaaag gacgcgataa aaaacaagtg tctgtttacc caatcgcaaa
    10681 ataatgccta gttaagactt tatgactatt cataaggtct tttattattg tcagagcttt
    10741 cattttgtaa caaaagacaa tatgttaaga gatttatcat taatactcat aaactttgtt
    10801 gttcttttac agtgaaatag tcctaattca tgaagaattt tccgcaagga tagttatttt
    10861 ttggtataat gaagggtact aaaatgagga agtgataaaa tgaatattca acaactgcgt
    10921 tatgttgttg ctatcgcaaa tagtggtact tttcgtgaag cagctgcaaa actatttgtt
    10981 agtcagccaa gtttatctgt tgccgttcgt gatttggaaa cagaattagg gtttcagatt
    11041 tttacgcgta caactactgg tgcagtgttg accaatcaag gcatgacttt ttatgagaat
    11101 gctttagaag ttgtgaaaag ctttgattca ttcgaaaaac agttttcgca atctgaagct
    11161 acagagcaag aattctcaat agcaagtcaa cattatgatt ttctacctcc tttaatcaca
    11221 gcgttttcaa aatgtaatga taattttagc tatttccgta ttttcgaatc aacaacaata
    11281 cgtatcttag atgaagttgc tcaagggaat agcgaaatag gtatcattta tatcaatagt
    11341 caaaataaaa aaggtttact tcagcggttg gacaaattgg ggttagagtt tgtagagctt
    11401 attcctttta aaactcatat ttatcttggg aaagaccatc ccttggctag taaaacatct
    11461 ctgataatga cagatttaga aggattacca accgtacgtt ttacacagga tagagatgat
    11521 tatcggtatt actcggagaa ttttgtggaa gtacttgata gttctgttac ctataatgtt
    11581 actgatagag caacattaaa tggtatttta gaaagaaccc aagcttatgc gacgggctca
    11641 ggattcttgg atagccgcag tgtgaatggt atcactgtta ttccattaga agatcatcta
    11701 gataaccaaa tgatttatat caaacgaaaa gatcgtaatt tatctcagat ggctttaaaa
    11761 tttgttgctg ttatggaaga gtattttaag ggatttaatt cataggagaa gtaatgagaa
    11821 agattattat accaataata actattttgt tgattgcttt aga
//
LOCUS       DATRNK010000060        11289 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.060, whole genome shotgun sequence.
ACCESSION   DATRNK010000060 DATRNK010000000
VERSION     DATRNK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11289
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.060"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(294..1070)
                     /locus_tag="VBC52_001642"
     CDS             complement(294..1070)
                     /locus_tag="VBC52_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6883211.1"
                     /translation="MLKRERLQKIIEKVNINGIVTVNEIMEELDVSDMTVRRDLDELD
                     KAGLLIRIHGGAQKVNASPIPQNYEKSNTEKYDIQTNEKLEIAQFAKQFINDGETIFI
                     GPGTTLEKLATQLLDFKIRVVTNSLPVFNILNQSSTLDLILVGGEYREITGAFVGSVT
                     INSIKSLNFSKAFVSSNGVFEKSIATYDEGEGEIQRIALNNSFEKFLLVDSQKFGKYD
                     FYTFYQLDDIDFVLTDHNIDNVVKEQYSSFTKILTNNNYM"
     gene            1279..1752
                     /locus_tag="VBC52_001643"
     CDS             1279..1752
                     /locus_tag="VBC52_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6883212.1"
                     /translation="MFAVTQDILFIDAHSQEELFDLVSKALIKQHYVSPDYRQAVKER
                     EREFPTGLKIDLKDGTPIQYAAIPHTETQYCLVDRIFYVKNSQPITFKHMINPEEECR
                     VQDFFFIINSRNSNQSDILSNLITFFITKGNLDRLHELGDNKEKINHYLIEKGVF"
     gene            1754..2059
                     /locus_tag="VBC52_001644"
     CDS             1754..2059
                     /locus_tag="VBC52_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6883213.1"
                     /translation="MIKILAACGAGVNSSHQIKDAIETQLGDRGYNVHCDAVMVKDIT
                     EEMVNKYDIFTPIAKTDLGFNVPIPVVEAGPILYRIPVMSEPVFTALEQVIKEHNLS"
     gene            2099..3547
                     /locus_tag="VBC52_001645"
     CDS             2099..3547
                     /locus_tag="VBC52_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO6883214.1"
                     /translation="MQVFLNIVNKFFDPVIHMGSGVVMLIVMTGLAMIFGVKFSKALE
                     GGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGINLSIIDVGWAPLATITWGSPYT
                     LYFLLIMLIVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYAKKNNLPTLLSVIIA
                     TVAIIFVGVLKIINSDLMKPTFDDLLGTGPTSPMTSTHMNYMMNPIIMVLDKLFDKVF
                     PGLDKYDFDAAKLNKAIGFWGSKFFIGMILGLVIGIMGNPVFSFAALGGWFSLGFTAG
                     ACLELFSLIGSWFIAAVEPLSQGITNFANGKMHGRRFNIGLDWPFIAGRAEIWACANI
                     LAPIMLVEAILLSKVGNGILPLAGIIAMGVTPALLVVTRGRLIRMITFGTLLLPLFLL
                     SGTMIAPFATELAKKVGAFPAGARAGSLITHSTLEGPMEKIFGYVIGKATTGQLSAII
                     TLIIFATAYLGLFMWYAKQMKRRNAEYAANQK"
     gene            3788..6238
                     /locus_tag="VBC52_001646"
     CDS             3788..6238
                     /locus_tag="VBC52_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEO6883215.1"
                     /translation="MTLTFGKPSITIRRLGVITSIYMMLGFILLCGLLHPSTIYSEEI
                     KSDINSFISIDDEKTTAPIFDSTLDKLTNSIEERQLELDSDTSDGLIVDDQKDKDMVV
                     VLGDSDSQEKLSPPLKSEIATEENRKQANLAELNATQPNNRTTYIIPESSHSIAEQQR
                     FLIESKGSSVALLNSDEFRKTAREDRGFERDKLRSLDIIPKGDLSTSNVIGNTDIASQ
                     ISLGFKKNAIQEHHLTKTFSQKDGKLSSVIEGMLAIGKEKVEKEIKYSGNLWQKLKAK
                     AHCLVCCVDNLNFEDIKSYFQYYCHLNHQLKLPKGAILSAKTEVYRGGDFGRKNKDNV
                     FGYRIPSLLKTEKGTLLAGADERIEQACDWGNIGMVIRRSEDDGVTWGKRETIVNLRN
                     NPRVPLVTSGDYSGSPINMDMALVQDTSSKTKRIFSIYDMFPEGRGVISIANTPEKEY
                     TQIGGQSYLNLYNNEKKSKVFTIRDKGIVYNFKGKKTDYHVITETTKSDHSNLGDIYK
                     GKQLLGNIYFTKHKTSPFRLAKSSYVWMSYSDDDGRTWSSPRDITASLRRRGMKFLGI
                     GPGKGIVLKWGPHAGRIIIPAYSTNWKSHLRGSQSSRLIYSDDHGKTWHTGKAVNDNR
                     VLSNGEKIHSLTMDNKKEQNTESVPVQLKNGDIKLFMRNLTGNLEVATSKDGGETWQN
                     HVKRYKEVHDAYVQLSAIRFEHDKKEYILLVNANGPGKKRQDGYARLAQVNRNGSFKW
                     LYHHHIQDGSFAYNSVQQLNNDQFGVLYEHREKHQNSFTLNYKVFNWSFLSQNTEKQG
                     TLWEKMAANWHVLFKFYL"
     gene            6485..6910
                     /gene="lacA"
                     /locus_tag="VBC52_001647"
     CDS             6485..6910
                     /gene="lacA"
                     /locus_tag="VBC52_001647"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEO6883216.1"
                     /translation="MTIIIGADAHGVELKEVIRQHLTSLGKEIIDLTDTSKDFVDNTL
                     AIVAKVNQKEDNLGIMVDAYGVGPFMVATKVKGMIAAEVSDERSAYMTRAHNNARMIT
                     LGSEIVGPGVAKHIVEGFVDGTYDAGRHQIRVDMLNKMC"
     gene            6931..7446
                     /gene="lacB"
                     /locus_tag="VBC52_001648"
     CDS             6931..7446
                     /gene="lacB"
                     /locus_tag="VBC52_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEO6883217.1"
                     /translation="MKIAVGCDHIVTYDKIAVVDYLKTKGYEVIDCGTYDNIRTHYPI
                     YGKKVGEAVASGKADLGVCICGTGVGINNAVNKVPGIRSALVRDLTSAIYAKEELNAN
                     VIGFGGKITGGLLMTDIIEAFIRAKYKPTKENKVLIEKIAEVETHNAHQEENDFFTEF
                     LDKWNRGEYHD"
     gene            7457..8389
                     /locus_tag="VBC52_001649"
     CDS             7457..8389
                     /locus_tag="VBC52_001649"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEO6883218.1"
                     /translation="MILTVTLNPSIDISYCLENFNMDTVNRVTDVSKTPGGKGLNVTR
                     VLSQLGDNVVATGLLGGHFGDFIRSGLDTLEIRHQFLSIGGETRHCIAVLHEGQQTEI
                     LEKGPHITKDEADAFLNHLKLIFDAATIITVSGSLPKGLPSDYYARLISLANHFNKKV
                     VLDCSGEALRSVLKSSAKPTVIKPNLEELTQLIGKPISYSLDELKSTLQQDIFRGIDW
                     VIVSLGARGAFAKHGNHYYQVTIPKIEVINPVGSGDATVAGIASALEHQLDDINLLKR
                     ANVLGMLNAQETLTGHINLTYYQELISQIQVKEV"
     gene            8391..9368
                     /gene="lacD"
                     /locus_tag="VBC52_001650"
     CDS             8391..9368
                     /gene="lacD"
                     /locus_tag="VBC52_001650"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEO6883219.1"
                     /translation="MGLTEQKQKHMEQLSDKNGIISALAFDQRGALKRLMAKYQSEEP
                     TVSQIEALKVLVAEELTPYASSMLLDPEYGLPATKVLDDNAGLLLAYEKTGYDTSSTK
                     RLPDCLDIWSAKRIKEEGADAVKFLLYYDVDSSDEVNEEKEAYIERIGSECVAEDIPF
                     FLEILSYDEKIADSSGIEYAKIKPRKVIEAMKVFSNPRFNIDVLKVEVPVNMDYVEGF
                     AQGETAYNKATAAAYFREQDQATLLPYIFLSAGVPAQLFQETLVFAKEAGAKFNGVLC
                     GRATWAGSVKEYVEKGEAGARQWLRTIGFQNIDELNKILQKTATSWKER"
     gene            9422..10318
                     /locus_tag="VBC52_001651"
     CDS             9422..10318
                     /locus_tag="VBC52_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEO6883220.1"
                     /translation="MAITIQNHELQVTLKALGATMTSITDSQGVEYLWQGDPTYWGGQ
                     APILFPICGSVRNDCVIYRPAQAPHFTGIIPRHGFVRHKTFDYDYISDSSVRFTIKSS
                     EETLINYPYRFSLEITYTLRNKSIDITYIVKNLESEKNMPYAIGAHPGFNCPLFEKEV
                     FSDYYLEFEQFETCTIPESFPDTGLLDLQARHPFLENQKQLSLNHALFEKDAITLDQL
                     RSKTVYLKSRNHAKGIQLDFDDFENLILWTSNNGGPFLALEPWSSLSTSIEESDILED
                     KQNIVRLNPKQSKQHSIRITIL"
     gene            10413..11264
                     /locus_tag="VBC52_001652"
     CDS             10413..11264
                     /locus_tag="VBC52_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6883221.1"
                     /translation="MDFKELFPEVITKQEVKQSEDYIIVEQDGHVLHFPKSSLTKREL
                     CLLQMTPSLEDASSVDSQNPWYRYLVEGRGRLPQSHSAVQFIFIEHQFTLSEELKDFL
                     SPLVINVEIIMTINQTQSVMILNQDNFFNATELLTDILPTIENDFNTRLRCYFGNSWT
                     HLQAVDWKELYEEEYKLFTLFLSHKAEQHYCRFPKMALWALANQSPMPSIKAKCLQHI
                     LDTSDTSAIIKALWQEQGNLAKTAKALFIHRNSLQYKLDKFTQSSGLNLKILDDLAYA
                     YLISMDT"
BASE COUNT         3778 a         1725 c         2191 g         3595 t
ORIGIN      
        1 agaagtgaaa taataaaaga gttgagtaaa cttagctgac aactaaaaac aaacaagtca
       61 ccgtacttgt ttgtttttag ttatgatttc tagcgtgcga agattttaat gatatatcta
      121 attcttcttt tgaacaacca ttgttttacg cttaaaaaga gagttaatgt atttttgttt
      181 gaggaagttt tcttgttttg acttttataa aacgactaaa gttataatca ctaaataaag
      241 agccactact aaaagctgta taacaggttt ctagtagtgg ctcacttgtt ttattacatg
      301 taattgttat tagttaaaat tttggtgaag ctactgtatt gctccttaac aacattatca
      361 atattatgat ctgttaatac aaaatcaata tcgtctaatt gataaaaagt ataaaaatca
      421 tatttaccaa atttttggct atcaactaaa aggaattttt caaaggaatt attaagagca
      481 atgcgctgga tttcaccttc cccctcgtcg taagtagcga tagacttttc aaaaacacca
      541 ttactactaa cgaaagcttt tgaaaaattg agtgatttaa tagagttaat agtaacagaa
      601 ccgacaaaag caccagtaat ttctctgtac tctcctccta caaggataag gtcaagtgtt
      661 gaactttgat tcagaatatt gaaaacagga aggctattgg ttactaccct aattttaaaa
      721 tcaagtaatt gcgtggctaa tttctctaag gttgttccag gcccaataaa gattgtttca
      781 ccatcattga tgaattgttt tgcaaattgg gcaatttcta atttttcatt tgtttgaata
      841 tcgtattttt cagtgtttga tttttcataa ttttgtggaa ttggtgatgc gtttactttt
      901 tgtgcaccac catggatgcg tattaacagt cctgctttat ctaattcatc caagtcacgc
      961 ctaacagtca tgtctgatac atcaagttct tccatgattt cattaacagt gacaatacca
     1021 tttatattga ctttttctat tattttttgt aatctttctc gctttaacat aatgacctct
     1081 cctacctctt cgttatatat atcataccat aaatagagct aaaataataa taattttgaa
     1141 aaaataaaca ataataaaca aaaataaata aaaaatgtct tgtaatgttt tattttgttt
     1201 tgtataatat tgatgtagat aagttgataa ggcttatttg ccttacaaca aaatgtaacc
     1261 gcttaactaa gaagaaaggt gtttgctgtg actcaagata ttttatttat tgatgctcat
     1321 tctcaggaag aattatttga cttagtatca aaagcattga taaaacaaca ctatgttagt
     1381 cctgactacc gacaggcagt aaaagaaagg gagagagagt ttccgacggg gttaaaaatt
     1441 gatttaaaag atgggactcc tattcaatat gctgctattc cacacactga aacgcagtat
     1501 tgtctagtag atagaatctt ttatgtcaaa aatagtcagc caattacttt caaacatatg
     1561 attaatccag aagaggagtg tagggttcag gattttttct tcatcattaa tagtaggaat
     1621 agtaaccaaa gtgatatttt atctaatttg attaccttct ttattacaaa aggaaatcta
     1681 gatcggctac atgagcttgg cgacaataag gaaaaaatta atcattattt aatagaaaaa
     1741 ggagtgtttt aacatgatca aaatattagc agcttgtggt gcaggcgtaa actcaagtca
     1801 tcaaattaaa gatgctatcg agacacaatt aggagataga ggttacaatg tacattgtga
     1861 tgctgttatg gttaaggata tcactgagga aatggtcaac aaatatgata tctttactcc
     1921 tattgctaaa actgatttag gttttaatgt accaattcca gttgtagaag caggacctat
     1981 tctttatcgc ataccagtta tgagcgaacc ggtctttact gctttagaac aagttatcaa
     2041 agaacacaac ctatcataac atatttaaaa ttggaattag tcttaaagga gaaggaacat
     2101 gcaggtattt ttaaatattg tcaataaatt ctttgatcca gttattcata tgggttcggg
     2161 agttgtgatg ctaattgtca tgacaggttt agccatgata tttggagtga agttttctaa
     2221 agcacttgaa ggtggtatta agttagctat tgctcttacg ggtattggtg ctattattgg
     2281 tattttaact ggtgcttttt ccgaatcact tcaagctttt gttaaaaata caggaatcaa
     2341 tctaagcatt attgacgttg gttgggctcc attagcaact attacatggg gatcaccata
     2401 tacgctttac ttcttattaa tcatgcttat tgtcaatatt gttatgattg ttatgaaaaa
     2461 aacggatacc ttagatgttg atatttttga tatttggcat ttatctatca caggtctttt
     2521 aattatgtgg tatgctaaaa aaaataatct accaacactt ttatctgtca ttattgcaac
     2581 tgtagctatt atctttgttg gtgttctaaa aattatcaat tctgatttga tgaaacctac
     2641 ctttgatgat ttattgggga caggtccaac ttctcccatg acttcaacgc atatgaatta
     2701 tatgatgaac cccattatta tggttttgga taagcttttt gataaagttt ttccagggct
     2761 agataaatat gactttgacg cggctaaatt aaataaagcc attggattct ggggatctaa
     2821 gttctttatt ggtatgattt taggacttgt catagggatt atgggcaacc ccgtgttctc
     2881 atttgcagca ttaggtggct ggttctctct cggttttaca gcaggtgctt gtttagagtt
     2941 gttctctttg attggttctt ggttcattgc tgcagtagaa ccactttctc aaggaattac
     3001 taattttgct aatggtaaaa tgcatggtcg tcgtttcaat attggtttgg actggccatt
     3061 tattgccgga cgtgcagaga tttgggcatg tgctaacatt cttgcaccga ttatgctagt
     3121 agaagctatt ttactctcta aagttggtaa tggtattcta ccgcttgctg gtattatcgc
     3181 aatgggcgta acccctgctt tactggttgt tacacgtggc cgtttaattc gtatgattac
     3241 ctttggaaca ttgctattac cgctatttct tttatcagga acaatgattg ccccatttgc
     3301 aactgaatta gctaaaaagg tgggcgcatt cccagctggg gcgcgagcag gttcacttat
     3361 cactcactct acactagaag gccctatgga gaaaatcttt ggttacgtta ttggtaaagc
     3421 gacaacaggt caattatcag cgattataac attaattatt tttgcgacag cttatcttgg
     3481 tttgtttatg tggtatgcta aacaaatgaa acgccgtaat gcagaatatg cagctaatca
     3541 aaaataaata gaataaacaa aaacctagga aatatcacat tctcctaggt ttttgatata
     3601 ttaaatgttt taaaaataat atatataatt aattttcgct cattttaata ataaaaaact
     3661 agtaaaatta taaaaatata ttgaaaatag cgataagttg gtatacaatc ctttttggaa
     3721 acattactga actcccattt ttctagagtt gatgtatgca accaatattt taagacttgg
     3781 agtttctatg acattaacct ttgggaaacc ttctataacg atacgccgtc taggagtgat
     3841 aactagtata tatatgatgc taggttttat cttattatgt ggacttttgc atcctagtac
     3901 aatttatagt gaggaaataa aatctgatat aaactctttt atatcaatag atgatgaaaa
     3961 aactactgct cccatatttg actctacttt agataagctt actaattcaa tagaggagag
     4021 gcagttagaa ttagattctg atacatcgga cggtttaata gtagatgatc aaaaagataa
     4081 agatatggta gtagtcttag gtgattctga cagtcaagaa aaattgtcac ctcctctaaa
     4141 atcggaaata gcgacagaag aaaatcgtaa acaggcaaat ttagcagaac taaatgcaac
     4201 tcaacctaat aatagaacta cctatattat acccgaaagc agtcattcca ttgcagaaca
     4261 acagagattc ctgatagaat caaagggttc ttcggttgca ttacttaata gcgatgaatt
     4321 tagaaagaca gcgagagagg atagaggttt tgaaagggat aagttgaggt ctttggatat
     4381 cattcctaag ggagatttat cgacaagtaa tgtcataggt aatacggaca ttgctagtca
     4441 gatatcgttg ggctttaaaa agaatgcgat acaggaacac catcttacta aaacattctc
     4501 tcaaaaggat ggaaagttat cgtctgttat agaggggatg cttgctattg gcaaagagaa
     4561 agtagagaaa gaaataaaat atagtggtaa tttatggcaa aaattaaaag ctaaggcaca
     4621 ctgccttgtt tgctgtgttg ataatttgaa ttttgaagat ataaaatctt attttcaata
     4681 ttattgtcat ctaaaccatc agctcaaatt acctaaaggt gctatacttt ctgctaaaac
     4741 agaagtatat aggggaggag attttgggag aaaaaataaa gataatgtgt ttggttaccg
     4801 tatcccctca ttattgaaaa ccgaaaaagg aactttactt gcaggagctg atgaaagaat
     4861 tgagcaagct tgtgattggg gaaacatagg aatggttatt cgccgtagtg aggatgatgg
     4921 tgtcacttgg ggaaaaagag aaactattgt caatctccgt aataacccta gagttccgct
     4981 agttactagt ggtgactata gtggctcacc tattaatatg gatatggcat tagttcaaga
     5041 tactagctct aagacgaaac gtattttttc aatatatgat atgtttccag aaggaagagg
     5101 cgttattagt attgctaaca cacctgaaaa agaatatacc caaatcggag gacagtctta
     5161 tcttaattta tataataatg aaaagaaatc gaaggttttt actatccgtg acaaaggtat
     5221 tgtatataat tttaaaggga aaaagactga ttatcatgtt ataacagaaa ctactaaaag
     5281 tgaccattca aatctagggg atatttataa gggaaaacag ctacttggaa atatatattt
     5341 tacaaaacat aaaacgtcac catttcgttt agcaaaatca agctatgtgt ggatgtcata
     5401 tagcgatgat gatggtagga catggtcatc acctagagat ataacagcaa gtcttcgtcg
     5461 gagaggcatg aaatttttgg gaataggacc tggaaaaggt atagttttaa aatgggggcc
     5521 acacgctggt cgtattatta ttcctgccta ttctacgaat tggaaatctc atctaagagg
     5581 ttcacaatct tcacgcctaa tttattcaga cgaccatgga aaaacgtggc atactggaaa
     5641 agcagtcaat gataaccgtg tactttctaa tggtgaaaaa attcactcat taacaatgga
     5701 taataaaaaa gaacaaaata cagaatccgt acctgttcaa ttgaaaaatg gggacattaa
     5761 gttatttatg aggaatctaa ctggtaacct agaagtagcc acaagtaaag acggcgggga
     5821 gacttggcaa aaccatgtta aacgatataa ggaagttcat gatgcttacg tccaactatc
     5881 agctattcgc tttgagcatg acaaaaaaga gtatatttta ttagtgaatg ctaatgggcc
     5941 aggtaagaag cgtcaagatg gatatgcacg tctagcgcaa gttaatcgaa atggtagttt
     6001 taagtggtta tatcaccatc acattcaaga tggttcgttt gcttacaact ctgttcaaca
     6061 acttaataat gatcaatttg gtgtccttta tgaacataga gaaaaacatc aaaatagttt
     6121 tactttaaat tacaaagttt ttaattggag ttttcttagt caaaatacag agaagcaagg
     6181 cactttatgg gagaaaatgg cagcaaattg gcatgttttg tttaaatttt atttatgatt
     6241 atggcgtgcg gaataaccgt tcctacaaat aaaaataatg agaagtatca aacttcgtga
     6301 tagctagcta gtagtgaatt ctagttgaca tagttatcac ggagtttttt tgtttatttc
     6361 gaacaaaaac aaaacaaaac attgacaaaa acaaaaataa gagatatgat gatattacaa
     6421 acaaaaacaa acaatatctt ccaactacgt acaattgtat taaaagtgaa ataaggagaa
     6481 agaaatgaca attattattg gggcggatgc gcatggtgtt gaacttaagg aagtgattag
     6541 acaacacctt acaagtttag gtaaggaaat tattgattta acggatacta gcaaagattt
     6601 cgttgataat accttagcaa tcgttgctaa agtgaatcaa aaagaagata acttaggaat
     6661 aatggttgat gcttacggtg ttggtccttt tatggtagcc actaaggtta aggggatgat
     6721 tgcagcagaa gtttcagatg aacgctcagc ttatatgact agagctcata ataacgctag
     6781 gatgattact ttaggttcag agattgttgg tccaggagtt gctaaacata tagtagaggg
     6841 ttttgttgat gggacttacg atgcaggtcg tcatcaaatt cgggtcgata tgttgaataa
     6901 aatgtgctaa gcgtaagaga ggtaaagcag atgaaaattg cggtcggttg tgatcatatt
     6961 gtaacttatg acaaaattgc agttgtggat tatttaaaaa caaaagggta tgaggttatt
     7021 gattgtggaa cttatgataa tatccgtact cactatccta tttatggtaa gaaagtagga
     7081 gaagctgtcg ctagtggaaa agccgactta ggtgtatgta tttgtggaac tggagttggt
     7141 attaataatg ctgttaataa agttccagga attcgctcag ctctggtacg tgatttgaca
     7201 tcagctatct atgctaaaga agaattaaat gctaacgtta tcggatttgg tgggaaaatt
     7261 actggtgggc tgttaatgac agatatcatc gaggcattta taagagctaa atataaacca
     7321 acaaaagaaa acaaagtatt aattgaaaag atagccgagg ttgagactca taatgcccat
     7381 caagaagaga atgacttttt cacagagttc ttagataaat ggaatcgtgg tgaatatcac
     7441 gactaaggag atatctatga ttttaacagt aactttgaac ccttctattg atatttcgta
     7501 ctgcttagaa aactttaaca tggatactgt aaatcgagtt accgacgtct ccaaaacacc
     7561 tgggggtaag gggttaaatg tgactcgtgt tctttctcaa cttggcgata atgtagtggc
     7621 aactggtcta ttaggggggc attttggaga tttcatccga agtggcttag atacattgga
     7681 aatcagacac caatttttaa gtattggtgg agaaacacgt cattgtattg cagttttaca
     7741 tgagggtcag cagactgaaa ttctagaaaa aggtccacat attactaaag atgaagcaga
     7801 cgcctttttg aatcatctaa aacttatctt tgatgctgca actatcataa cagtatcagg
     7861 aagtttacca aagggactcc cctcagatta ttatgctagg ttaatctccc tagcgaatca
     7921 ctttaataag aaagttgttt tagactgttc aggagaagca ttgcgttcgg ttttaaaatc
     7981 tagtgctaag ccgaccgtca ttaaacctaa ccttgaagaa ttaacacaat taattggaaa
     8041 gccgattagc tatagtttag acgaactaaa atctacctta cagcaagata tttttagagg
     8101 aattgattgg gttattgttt ctttaggtgc taggggagct tttgctaaac atgggaatca
     8161 ctactatcaa gtgacgattc caaaaataga agttattaat ccagttggtt caggggatgc
     8221 aacggttgca ggaatcgcgt ctgctttaga acatcaacta gatgatataa atttgcttaa
     8281 gagagctaat gtcttgggta tgcttaatgc acaagaaaca ttaacagggc atatcaatct
     8341 gacctactat caagaattaa tcagtcaaat ccaagtgaaa gaggtatagg atgggattaa
     8401 cagaacaaaa acaaaaacat atggaacaat tgagtgataa aaatggtatt atttcggccc
     8461 tggcttttga ccaacgtgga gcattgaagc gattaatggc aaaatatcaa tctgaggagc
     8521 ccactgtttc tcaaatagaa gcacttaaag ttttggtagc tgaagaatta accccttacg
     8581 cttcatcaat gttgttagat ccagaatatg ggttgccagc tacgaaagtt ttagatgaca
     8641 atgccggtct tttacttgcg tatgaaaaaa cgggttatga taccagtagt accaaacgac
     8701 tcccagactg tttagacatt tggtctgcga aacgcattaa agaagaggga gcagacgcag
     8761 ttaagttctt actttattat gatgttgata gttcggatga agtaaacgaa gaaaaagaag
     8821 cttatattga acggattggt tctgaatgtg ttgcagaaga tattcctttt ttccttgaaa
     8881 ttttgtcata tgatgaaaag attgcggata gctcaggtat tgagtatgct aaaattaagc
     8941 cacgtaaggt tattgaagct atgaaagttt tttcaaatcc tcgatttaat attgatgtct
     9001 tgaaggtgga agtgcctgtc aatatggact atgtagaagg ttttgcgcaa ggagagactg
     9061 cttataataa agcgacagct gcggcttatt ttagagaaca agatcaggcg acactactcc
     9121 cctacatttt tttgagtgca ggagttcctg ctcagctttt tcaagagact cttgtgtttg
     9181 ctaaagaagc tggtgctaaa tttaacggtg tcctttgtgg tagagcaact tgggctggaa
     9241 gtgttaaaga gtatgttgaa aaaggagaag ctggtgcacg tcaatggtta cgtacaatag
     9301 gttttcaaaa tattgatgaa ctaaataaaa ttttgcaaaa aacagcaact tcttggaagg
     9361 aaagataatt gctagggaaa agctggtaaa gcaattattg ctctttatga aaggaaaaat
     9421 aatggctatt acgatacaaa atcatgagtt gcaagtgaca ttaaaagcgt taggtgcaac
     9481 aatgacttct attacagata gtcaaggcgt tgagtacctt tggcaagggg accccactta
     9541 ttggggtggg caagctccta ttttatttcc tatatgcgga agtgttcgaa atgattgcgt
     9601 catttacaga ccagctcaag ctccacattt tacgggtatc ataccaaggc atggctttgt
     9661 acgtcacaaa acattcgact atgattacat atcggattct tctgttcgtt ttacaattaa
     9721 aagtagtgaa gaaacgttga taaattatcc ttatcgtttt agtttagaaa taacttacac
     9781 cttaagaaat aaaagcattg acattacata tattgtcaaa aacttagaat cagaaaaaaa
     9841 tatgccatat gctatcggag ctcacccagg ctttaattgc cctttgtttg aaaaggaagt
     9901 atttagtgat tactatctag agtttgaaca gtttgaaact tgcacaattc ctgagtcgtt
     9961 tccggataca ggcttgttag atttacaagc tagacatcct tttttagaaa atcaaaaaca
    10021 attatctctt aatcatgctt tatttgaaaa agatgctatt actttagacc aactacgatc
    10081 taagacggtt tacttaaaat ctagaaatca tgcaaaagga atccaacttg actttgatga
    10141 ttttgagaat cttattcttt ggacaagtaa caatggtggt ccttttttgg ctttggaacc
    10201 ctggagttct ttgtcaactt ctattgaaga gtctgatatt ttagaagata aacaaaatat
    10261 cgtacggctt aatccaaagc agtcgaaaca gcattctatt cggatcacta ttttataaca
    10321 gaaattttct gaggagacat tctcctcttt ttctgtgctc aaaaagaggt tataaatatg
    10381 atataatgaa caaaactgta gtggaggatg ccatggattt taaggaactg tttccagaag
    10441 ttattactaa acaggaagtt aaacagagtg aggattatat catcgttgag caagatggtc
    10501 atgtcctcca ctttccaaaa tcttcgctaa ctaagcgtga actatgctta ttacaaatga
    10561 caccaagcct ggaagatgcg agttcagtgg attctcagaa tccttggtac cgctatctag
    10621 ttgaaggtcg tggaaggttg ccccaatctc attcggcagt ccaatttatt tttattgagc
    10681 atcaatttac cttatcagaa gaacttaagg atttcttgtc gcctttagta ataaacgtag
    10741 aaataattat gacaattaat caaacccaaa gtgtcatgat tttaaatcag gataatttct
    10801 ttaatgctac ggaattacta acagatattt taccaacgat tgaaaatgac tttaacacgc
    10861 gtttaaggtg ctattttggg aatagttgga cgcatctaca agcagtggac tggaaagaat
    10921 tatatgaaga agaatataaa ctttttacgc tttttcttag tcacaaggcc gaacaacatt
    10981 actgtcgatt tccaaagatg gctttatggg ctttggcaaa tcaatcgccc atgccttcta
    11041 ttaaagcaaa atgtttacaa catatccttg atacgagtga tacttcggct ataataaaag
    11101 ccttgtggca agagcaaggg aatcttgcta aaacggctaa ggcacttttc attcatcgga
    11161 attcattgca atataaatta gataaattta cccaatcttc gggacttaat ttaaaaatat
    11221 tagatgattt agcctatgcc tatttaattt cgatggatac ctaaaattta gttagtttgc
    11281 acaagaaat
//
LOCUS       DATRNK010000061        10957 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.061, whole genome shotgun sequence.
ACCESSION   DATRNK010000061 DATRNK010000000
VERSION     DATRNK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10957
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.061"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            347..634
                     /locus_tag="VBC52_001653"
     CDS             347..634
                     /locus_tag="VBC52_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEO6883222.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILSDNGATVVESKDWE
                     KRRLAYEIQDFTEGLYHIVNVEAEDAVALNEFDRLSKINGDILRHMIVKVD"
     gene            646..1137
                     /locus_tag="VBC52_001654"
     CDS             646..1137
                     /locus_tag="VBC52_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO6883223.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFSLAVNRNFKNQSGER
                     EADFINCVIWRQQAENLANWAKKGALVGITGRIQTRNYENQQGQRVYVTEVVAESFQL
                     LESRATREGGSSNSYNNGGYNNAPSNNSYSASSQQTPNFSRDESPFGNSNPMDISDDD
                     LPF"
     gene            1182..1421
                     /locus_tag="VBC52_001655"
     CDS             1182..1421
                     /locus_tag="VBC52_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEO6883224.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            1591..2535
                     /locus_tag="VBC52_001656"
     CDS             1591..2535
                     /locus_tag="VBC52_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6883225.1"
                     /translation="MKQMFLSTAIEFKEIETFEPGAWINLVNPSQEESEQVANQFGID
                     IDDLRAPLDVEETSRISVEDDYTLVIVDVPTYEERNNKSYYMTIPMGIIVTDNAVITT
                     CLEHLTLFDHFYRRRVKNFYTFMKTRFVFQLLYRNAELYLQALRTIDRQSDKIEAQLE
                     SATRNEQLIDMMELEKSIVYLKASLKFNERIVKKLTSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMANIYENVLNAMTETTASIIGNNQNTIMKTLALVTMTLDIPTVIFSAYGMNF
                     QNNWMPLNGLAHGFIYVVLLAFLMSSFVVFYFIRKKWF"
     gene            2560..3231
                     /locus_tag="VBC52_001657"
     CDS             2560..3231
                     /locus_tag="VBC52_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HEO6883226.1"
                     /translation="MTLQDLTKKNQEFVHIATNQLLADGKSDAEIKAILEEHLPEIID
                     NQKKGITARSLLGAPTTWAASFTERPEDKARVSVQKNTNPWLMWLDTSLLFLGLVTAL
                     NGLMLLFGQSNVNTGLISILTLGFGGGAAMYVTYYYIYRHMGKPKSERPGWLKSFAVL
                     ALVMLVWFALFAVVPLLPATINPKLPEAVLFIIALASFSLRFYLQRKYNIQSSMAPVA
                     PQQRR"
     gene            3362..6190
                     /gene="uvrA"
                     /locus_tag="VBC52_001658"
     CDS             3362..6190
                     /gene="uvrA"
                     /locus_tag="VBC52_001658"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEO6883227.1"
                     /translation="MQDKLMIRGARAHNLKNISVDIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSVEQIVDKVLSLPERTKMQILAPIIRRKK
                     GQHKSTFEKIQKDGYVRVRIDGDIHDVTEVPELLKSKMHNIDIVVDRLINKEGIRSRL
                     FDSVEAALRLSDGYVVIDTMDGNELLFSEHYSCPECGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDIDLVIPDKSKTLREGALVPWNPISSNYYPTMLEQAMTQFGVDMDTP
                     FEKLSKAEQDLALYGSGEREFHFHYINDFGGERNIDLPFEGVVNNINRRYHETNSDYT
                     RNVMREYMNELKCNTCHGYRLNDQALCVRVGGEEGLNIGQVSDLSIADHLELLETLRL
                     SSNEQLIARPIIKEIHDRLSFLNNVGLNYLNLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYVLDEPSIGLHQRDNDRLIDSLKKMRDLGNTLIVVEHDEDTMMAADWLIDVGPGA
                     GAFGGEIVASGTPKQVAKNTKSITGQYLSGKKVIPVPSERRVGNGRFLEIKGAAENNL
                     QNLDVKFPLGKFIAVTGVSGSGKSTLINSILKKAVAQKLNRNSDKPGKYVSLEGIEYV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     ESCSGDGIIKIEMHFLPDVYVPCEVCHGTRYNSETLEVHYKEKNIAQILDMTVNDAVT
                     FFAAIPKIARKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHADDIARLLKVLDRFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGIGGGQ
                     IVAIGTPEEVAENSKSYTGYYLKEKLAR"
     gene            6526..8025
                     /locus_tag="VBC52_001659"
     CDS             6526..8025
                     /locus_tag="VBC52_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO6883228.1"
                     /translation="MTTKAKHDIHGKPYNRTAMITLLLIATFAGVLNQTSLGTAIPTL
                     MNSFNISLSTAQQATTWFLLANGIMIPVSAYLATRFSTKWLYVTSYVVLLIGLLMTTL
                     APTSNWNLFLVGRIIQAISVGISMPLMQVVMVNVFPPEQRGAAMGLNGLVVGLAPAIG
                     PTLAGWILKQEFHFAGHDLTWRAIFLLPLLILTVTTILSPFVLKDVVDNKSVKLEVPS
                     LILSIIGFGSFLWGFTNVATYGWGDIGYVISPIMVGIIFIALFIHRQLKLETPFLDIR
                     VFKNKQFSVTTAAIALSMMAMMGVEMMLPLYLQNVHGLSALDSGLALLPGALMMGIVS
                     PISGAVYDKVGARRMAMIGFTILGVATLPFVFLTTTTPDHFITLLYAVRMFGIAMVMM
                     PLTASAMSALPPHEAAHGTAANNTARQIASAVVVALLSSVAQNIITNNKPSKDLLTMN
                     PLKYANQMLNASLDGFHVSFAIGFVFAVLGLLVSLFLRKGKIIETEKEV"
     gene            8027..8743
                     /locus_tag="VBC52_001660"
     CDS             8027..8743
                     /locus_tag="VBC52_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEO6883229.1"
                     /translation="MIILIIALATILTFVTWMLIHPKALRYILGLLSLALLFASVWML
                     TDHFVNHTGMTIETKTKTREIYSAGDSKAPFGLMIYKEIGTHSSNNVLVYRTDLNQKE
                     AKAHFVPNTKEVISAVKKSASYQLADVKKATVTTKTKRYVWKDSLSKLLYGFAGENGE
                     LISQKSVAKIPRKTWLVLTKEQSEHLESAAAKLKEQMTLQAKVNPVKAMEIKTLAKTN
                     PEAYAEMQVKALKEALNIKE"
     gene            8843..9910
                     /locus_tag="VBC52_001661"
     CDS             8843..9910
                     /locus_tag="VBC52_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO6883230.1"
                     /translation="MGFEQRLTRCQTAISQLSCDALLITNLTNIFYLTGFSGTNATVL
                     ISPKHRIFVTDSRYALIAKNTVREFDIIISREPLAAILKIIRDDALMAIGFETDISYH
                     MYKHMVEVFEDYSLIEAPSVVEKLRMIKDATELAIIRQACRISDQAFLDVLDFIKPDR
                     TTELQVANFLDFRMRELGATSPSFDFIVASGHRSAMPHGVASQKTIQSGETLTLDFGC
                     YYQHYVSDMTRTIHIGHVTDEERGIYDIVLKSNQAIIDNVKSGMKRCDYDYLARQVIE
                     NSGYGNHFTHGIGHGMGLDVHEIPYFGKSEGVIASGMVVTDEPGIYLDNKYGVRIEDD
                     LLITETGCEVLTSAPKELIVL"
     gene            9922..10374
                     /locus_tag="VBC52_001662"
     CDS             9922..10374
                     /locus_tag="VBC52_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEO6883231.1"
                     /translation="MNRLSWEDYFMANAELISKRSTCDRAFVGAVLVKNNRIIATGYN
                     GGVSETDNCNEVGHYMEDGHCIRTVHAEMNALIQCAKEGISTNNTEIYVTHFPCINCT
                     KALLQAGVKKITYKANYRPHPFAIELMEAKGVAYVQHDVPEVTLGMEE"
BASE COUNT         3530 a         1748 c         2177 g         3502 t
ORIGIN      
        1 taaaatcgga gacactgaat ttttctccaa cgaaaactta aaccaataaa gaattcttta
       61 aataatatgt tgaaggtgag gtgtaaacct cgccttttta ttttttaatt gaattataga
      121 atggggattg aacataagtc agtaaaagaa aatggctaca taggtttgat ataaggcata
      181 tttcaataaa gaaaaataaa aaatgtcttt aaatgatatc agggaaataa aaacataaaa
      241 accttgcatt attaacttat ttctactata ataatatctt gtgagcataa cctcacttac
      301 tcatgaaaaa ttcatgagtc attagaccaa aaggaggaac atatcaatgg ctaaatacga
      361 aattctttat attattcgtc caaacattga agaagaagct aaaaacgctt tggtagcacg
      421 ttttgattct atcttatctg acaacggtgc aactgttgtt gaatcaaaag attgggaaaa
      481 acgtcgtctt gcatacgaaa tccaagattt cactgaagga ctttaccata tcgttaacgt
      541 tgaagctgaa gatgcagtag ctcttaatga gtttgaccgt ctttcaaaaa tcaacggtga
      601 cattcttcgt cacatgatcg ttaaagtaga ctaagaaggt agtttatgat taataatgta
      661 gtacttgtag gtcgcatgac ccgtgatgca gaacttcgtt atacaccaag taatcaagcg
      721 gtagccactt tttcacttgc agttaatcgt aattttaaaa atcaatctgg cgaacgtgag
      781 gctgatttta ttaactgtgt tatttggcgc caacaagctg aaaacttggc taactgggca
      841 aaaaaaggtg ctttggttgg aattacaggt cgtatccaaa cacgtaatta tgaaaaccaa
      901 caaggtcaac gtgtctatgt aacagaagtt gttgcggaaa gtttccaatt attggaaagt
      961 cgtgctacac gagaaggtgg ttcatctaac tcttataata acggtggcta taacaatgct
     1021 ccgtcaaata atagttattc agcttcttct caacaaacgc ctaattttag tcgtgatgag
     1081 agtccgtttg gtaactcaaa tccaatggat atttcagatg atgatctacc attctaatat
     1141 aatagatgga cagaatttaa aactataaag gagaaataaa catggctcaa caacgtcgtg
     1201 gcggattcaa acgccgtaaa aaagttgatt atatcgcagc taacaagatt gaatatgttg
     1261 attacaaaga tacagagctt cttagccgtt tcgtttcaga acgtggaaaa attttaccac
     1321 gtcgtgtaac aggaacttca gctaaaaacc aacgtaaagt aacaacagca atcaaacgtg
     1381 ctcgtgttat ggctcttatg ccttacgtaa atgaagatta atataaaata gccctagcgg
     1441 ctattttttt attcttttta ccattattat tggtatttac gagtcaaata atagaaaaat
     1501 ttaggatacc accgtattaa ccgtgcaaaa caaatggttt tcttgtataa tactaaggta
     1561 tggatttttt ataacaaagg aggaatcgcc atgaagcaaa tgtttctttc aacggcaatt
     1621 gaatttaaag aaattgaaac attcgagcca ggtgcttgga taaatctggt taatccctcc
     1681 caggaagaat ccgaacaagt agctaatcaa tttggtattg atattgatga tttacgtgct
     1741 cccctcgatg tggaagaaac ttcccgtatc tcggttgaag atgattatac tttagttatt
     1801 gttgacgttc ctacctatga agaacgcaat aacaaaagtt attatatgac tattcctatg
     1861 ggcattatcg ttactgacaa cgctgttatt actacttgcc tagaacattt gacgctgttt
     1921 gatcactttt atagacgacg tgtcaaaaat ttttatacct ttatgaagac acgttttgtt
     1981 ttccaattgc tttatcgcaa tgccgaatta tacttacagg ctttgcgtac tatcgatagg
     2041 cagagcgata aaattgaagc acaactagag agtgcaactc gaaatgagca attgatcgac
     2101 atgatggaat tggaaaaatc tattgtctat ctaaaggcct ctttaaaatt taatgagcga
     2161 atcgtcaaaa aattgaccag tagtaccagt agtttaaaaa aatacattga agatgaagat
     2221 ctgcttgaag atacattgat cgaaactcag caggctattg aaatggcaaa tatctatgaa
     2281 aatgtcttaa atgccatgac agaaacgact gcttcaatca tcggtaataa tcagaatacc
     2341 attatgaaaa ccttagccct agtaaccatg acattggata ttccaacggt tattttttcg
     2401 gcgtatggga tgaactttca aaataattgg atgcctttga atggtctcgc acatggattt
     2461 atctatgttg ttcttttggc ctttttgatg agttcatttg ttgtatttta ttttattcgt
     2521 aaaaaatggt tttaatagta tattaatcaa ggagttcaca tgactttaca agacttaact
     2581 aaaaagaatc aagagtttgt tcatattgca actaatcaac ttttagctga tggaaaatca
     2641 gatgcagaaa tcaaagctat tttagaagaa catctcccag agattataga taatcagaaa
     2701 aaaggaatta ccgcacgctc actcttagga gcaccaacta cttgggcagc ctcattcact
     2761 gagcgacctg aggataaagc tagagtatct gtacaaaaaa ataccaatcc ttggttgatg
     2821 tggttagata cttccttact atttcttggc ttagtaactg ctttgaatgg attaatgttg
     2881 ttatttggtc aatctaatgt caatacaggg cttatttcaa ttttaactct tggttttggt
     2941 ggtggtgcgg ccatgtacgt tacttattac tatatttatc gccatatggg taaaccaaaa
     3001 tctgaaagac caggctggct taaatctttc gcagtactgg cactagttat gttagtttgg
     3061 tttgccttat ttgcagtagt acctcttcta cctgccacta ttaatcctaa acttcctgag
     3121 gctgttttgt ttattattgc tttagcatcg tttagtttgc gcttttattt acaacgtaaa
     3181 tataatattc aaagctcaat ggcaccagtg gcaccacaac aaagaagata ataacaaaga
     3241 gtatgaacgt attagttcat ctcttttttc tatgaaattt aaaactaaaa aatactgact
     3301 ttattaaaat ttgatataat aagtcaacac tattcaaaaa ggagtgctga ggcacaacat
     3361 tatgcaagat aaattaatga taagaggagc aagagctcac aatttaaaaa atataagtgt
     3421 cgacattcca cgagacaagt tagttgtcgt tacgggacta tcaggatcag gtaagtctag
     3481 tttggccttt gacactatct atgcagaagg acaacgtcgt tacgttgaaa gtctttctgc
     3541 ttacgcgcgc caatttttgg gaaatatgga aaagcctgat gtagattcta ttgatggcct
     3601 tagtccagct atttctattg atcagaaaac aacaagtaag aatcctcgct ctactgttgg
     3661 gactgtaaca gaaattaacg attatttacg tttgctttat gctcgtgttg gaactccata
     3721 ttgtatcaat ggtcacggag ccatcacagc atcttctgta gagcaaattg tggataaggt
     3781 actatcgctt ccagagcgaa caaagatgca aattttagcc ccaattatac gtcgaaaaaa
     3841 aggacaacac aaatcaacat ttgagaaaat acaaaaagat ggttacgttc gtgttcgtat
     3901 tgatggtgat attcacgatg ttactgaggt gcctgaatta ttaaaaagta agatgcacaa
     3961 tattgatatt gtagtagatc gattaattaa taaagagggc attcgttccc gcttatttga
     4021 ttccgttgaa gcggctctac gtttgtcgga tggttatgtc gttattgata caatggatgg
     4081 caatgagtta ttgttttcag agcattattc ttgtcctgaa tgtggtttta ctgttcctga
     4141 attagagcca cgcctttttt cttttaatgc accctttggt tcttgtccga cctgtgatgg
     4201 cttaggaatt aagttagaag tagatataga tttggttatt ccagataaaa gcaagaccct
     4261 tagagaaggg gccttagttc cttggaatcc aatttcatca aattattatc caacgatgct
     4321 agagcaagct atgactcaat ttggtgttga tatggatacg ccatttgaaa aattaagcaa
     4381 ggcggagcag gacctagccc tatatggttc aggcgaacga gagttccatt tccattatat
     4441 taacgatttt ggaggcgaga gaaatattga tttgcctttt gaaggggttg tcaataatat
     4501 taatcgtcgt taccatgaaa ctaatagcga ttatacacgc aatgttatgc gagaatatat
     4561 gaacgagcta aaatgtaaca cttgtcatgg ctatcgtctc aatgatcaag cactttgcgt
     4621 aagagttggg ggagaagaag gtcttaatat tgggcaagtc tcagatttat cgatagcaga
     4681 ccatttagaa cttttagaaa cgttgcgttt atcttcaaat gaacaattaa ttgctcgacc
     4741 aattatcaaa gaaatccatg accgcctaag tttcttaaat aatgttgggc ttaattattt
     4801 aaatctgtca cgttctgcag gaacactttc cgggggcgag agtcaacgta ttcgtttggc
     4861 tactcaaatt gggtctaatt tatcaggtgt cctttatgta cttgatgagc cgtccattgg
     4921 acttcatcag cgagacaatg accgtttgat tgatagtctt aagaaaatgc gtgatttggg
     4981 taatacgctt attgttgttg aacatgatga agatacaatg atggcagctg attggttaat
     5041 cgatgtggga cctggtgctg gtgcgtttgg tggtgagatt gtggcctctg gcacaccgaa
     5101 gcaagtagct aaaaatacta aatccattac aggacaatat ttgtcaggta agaaagtgat
     5161 tcctgtgcca tctgaacgaa gagtaggcaa tggtcgcttt ttagaaatca aaggtgcggc
     5221 agaaaacaac ctacaaaatt tagatgtcaa gttccctctc ggtaaattta ttgccgtaac
     5281 tggtgtctca ggttctggaa aatcaacctt aattaatagt attttgaaaa aagctgttgc
     5341 gcagaagctt aaccgtaact cagataaacc aggtaaatat gtttctttag aaggcattga
     5401 atatgttgat cgattgattg atattgacca aagtccaatt ggtcggactc cacgttctaa
     5461 tcccgcaacc tatacaggtg tttttgatga tattcgtgat ctttttgctc aaactaatga
     5521 ggcaaaaatt cgaggttata aaaaaggacg cttctcattt aacgtcaaag gtggacgttg
     5581 tgagtcttgt tctggcgatg gtatcatcaa aattgagatg cattttttac cagatgtcta
     5641 cgttccttgt gaggtatgcc atggcacgcg ctataatagt gagactctgg aagtacatta
     5701 taaagaaaaa aatattgcac aaattcttga tatgacagtg aatgatgcag ttacattttt
     5761 tgcagctatt cctaaaattg ctcgtaagtt acagactatt aaggatgttg gtttgggata
     5821 tgtcactttg gggcaaccag ctacaacctt atcaggagga gaagctcagc gaatgaagct
     5881 ggcaagtgaa ttacataagc gttcgacagg taagagtctt tacatattag atgaaccaac
     5941 aacggggctt catgcagatg atattgctcg tttacttaag gttttggatc gttttgtaga
     6001 tgatggtaat acagtgcttg ttattgagca taaccttgat gttattaaaa ctgctgacca
     6061 cattattgat ctcggccctg aaggaggtat tggtggcgga caaattgtag ctatcggaac
     6121 accagaagag gttgctgaaa attctaaatc ctatacagga tattatttaa aagagaagtt
     6181 agcaagatag tttattggga gctgagtaat attactcagt tcctttttgt aagctaacat
     6241 tatgtagaag tcattaaatg gagaacgctt tattaggaaa aatagtgggg agatatttta
     6301 gaaaaaggaa ataatgatgt tttgaaaaaa atagtttcta tgagaatctt tttcttgaca
     6361 ataaaattta gctattataa aatagttctc acgataacta aaataatgat tttttcagaa
     6421 aataatattt tttcttgatt aaaaaatcaa aaaataacct tataagacct atttgttatt
     6481 cttaaaagat attagttaaa atcagaaata gagattggag ataaaatgac tacaaaagca
     6541 aaacatgata ttcatggcaa accatataac cgtacagcca tgatcacctt attgttgatt
     6601 gctacatttg caggggtgtt aaatcagaca agtttgggaa cggctatccc aactttgatg
     6661 aatagcttta acatttcgtt gtcaacggct caacaagcaa caacatggtt tttattagcg
     6721 aatgggatta tgattcctgt ttcagcttat ttagcgacac gtttttcaac aaaatggttg
     6781 tatgtgacat cctacgttgt tttacttatt ggactgttaa tgacgacgtt agcacctaca
     6841 tctaactgga accttttttt agtaggacgt attattcaag cgatttctgt aggaatttct
     6901 atgcctttga tgcaggttgt tatggtgaat gtttttcctc cagagcagcg tggtgccgct
     6961 atgggactta atggtttggt ggttggtctt gccccagcta ttgggcctac attagcaggg
     7021 tggattttaa aacaagaatt tcattttgca gggcatgatt tgacatggcg tgcaattttc
     7081 cttcttcctt tactaatttt aacggtcact acaattttat ccccctttgt tctaaaagat
     7141 gtggttgata ataagtcagt taaattggaa gtgccttccc ttatcctttc aataattggt
     7201 tttggtagct ttttgtgggg cttcacaaat gtggcaactt atggatgggg agatattgga
     7261 tatgttattt ctcctattat ggttggtatt atttttatcg ccttattcat tcatcgtcaa
     7321 ttaaaactag aaacaccgtt cttagatatc cgtgttttca aaaataaaca attctcagta
     7381 acaacagcag ctattgcact ttcaatgatg gcgatgatgg gtgttgaaat gatgctaccg
     7441 ctttacttgc aaaacgttca tggtctctct gcacttgatt ctggtttagc tttattacca
     7501 ggtgccttga tgatggggat agttagtcca atttctggag ctgtatatga taaagttggt
     7561 gcaagacgta tggctatgat tggttttacc atactaggtg tagcgacgtt accttttgtt
     7621 ttcttaacga ctacaacacc agatcatttt attaccctct tatatgcagt acgtatgttt
     7681 ggtattgcta tggttatgat gccattgaca gcaagtgcga tgagcgcact tccaccacat
     7741 gaggccgcac atggaacagc agctaataat actgctcgtc aaattgcttc agcagttgta
     7801 gttgcattgc tttcaagtgt tgctcaaaat attatcacca acaataaacc atcaaaagat
     7861 ttgctaacaa tgaatccttt aaaatatgca aatcagatgt tgaatgctag tcttgatggc
     7921 ttccatgttt cttttgccat tggttttgta ttcgcggttc ttggtttact ggtatctctt
     7981 ttcctgagaa aagggaaaat tattgagacg gaaaaggagg tatagcatga ttattttgat
     8041 tattgcactg gcaactattt tgacatttgt cacatggatg ttgattcatc caaaagctct
     8101 acggtatatt ctaggtcttc tctcattagc tttgttattt gcctcagttt ggatgttaac
     8161 ggatcacttt gttaatcata caggtatgac tattgaaact aagacaaaga cacgtgagat
     8221 ttatagtgcg ggtgatagta aggctccatt tggtctcatg atttataaag agattggtac
     8281 tcattcgagt aataatgttt tagtttatcg aacagattta aatcaaaaag aagcaaaagc
     8341 tcactttgtt ccaaatacaa aagaagtgat aagtgctgtg aaaaaatctg ctagctacca
     8401 attagctgat gtgaaaaaag ccacagtaac aacaaagact aaacgttatg tgtggaaaga
     8461 tagtctttcg aaactacttt atggctttgc aggtgaaaat ggagaattga tttctcagaa
     8521 atcagttgct aaaataccac gaaaaacttg gctagttttg actaaagagc agtctgaaca
     8581 cttagaatcg gctgcggcta aactaaaaga acagatgact ttgcaagcaa aagtaaaccc
     8641 agtgaaagct atggagataa agacattagc taagacaaat ccagaagcgt atgctgagat
     8701 gcaagttaaa gcattaaaag aagctcttaa tataaaagaa tagtgaagta aaagcaactg
     8761 aaatgagttg cttttcttat agctaaatgt cttaaaagat gataaaatta acctaattaa
     8821 ggttagaata gtgaggtttc ttatgggttt tgaacaacgt ctaactagat gtcaaacagc
     8881 aatttcacaa ttatcatgtg acgcactctt aataacgaat ttaacaaata ttttttattt
     8941 gactggtttt tcaggaacta atgcaacggt actcattagt ccaaaacacc ggatttttgt
     9001 tacagattca cgatacgcct tgatagcaaa gaatactgta agagagtttg atattatcat
     9061 tagcagagaa ccacttgcgg ctattttgaa aattattcga gatgacgcct tgatggcgat
     9121 tggttttgaa actgatatct cttatcatat gtacaaacat atggtggaag tttttgaaga
     9181 ttatagcttg atagaagctc catctgttgt tgaaaagtta cgtatgatta aagatgcaac
     9241 agaacttgcg atcatccgtc aagcctgtcg tatttcggac caagcttttt tagatgtgtt
     9301 ggattttata aaacctgatc gaacaactga attgcaagtt gctaattttt tagactttcg
     9361 catgagagaa ttaggagcaa ctagtccttc atttgatttt attgtagcat ctggacatcg
     9421 atcagcgatg ccacatgggg ttgcgagtca gaagactatt caatcgggag aaactctgac
     9481 tttagatttt ggttgttact atcagcacta tgttagtgat atgacacgta cgattcatat
     9541 cggtcatgtc actgatgagg aacgcgggat ttacgatatc gttttgaaaa gtaatcaagc
     9601 tattattgat aatgtaaaat caggaatgaa gcgttgtgat tatgattact tggcgcgaca
     9661 agtaattgaa aattctggat acggtaatca ttttacccac ggtatagggc atggtatggg
     9721 gcttgatgtt catgaaattc cttattttgg taagtcagag ggagtgattg catctgggat
     9781 ggtagtgact gacgagccag gaatatatct cgataataaa tacggtgttc gtattgaaga
     9841 tgatttatta ataacggaaa caggttgcga agtattgaca tctgcaccta aagaattaat
     9901 tgtactttaa aggaaaatag aatgaataga ctttcttggg aagattattt tatggcaaat
     9961 gcagagttga tttctaaacg ttctacctgt gaccgtgctt ttgttggagc tgttttagtc
    10021 aaaaataatc gcatcattgc tacaggttat aacggaggag tctctgagac agataactgt
    10081 aacgaagtag gacattatat ggaagatggt cactgtatta ggactgttca tgctgagatg
    10141 aatgccctta ttcagtgtgc taaagaaggt atttctacta ataatacaga aatttatgtg
    10201 acgcattttc cttgtatcaa ttgcaccaag gcgctcttgc aagccggggt taagaaaatt
    10261 acttataaag ctaattatcg cccacaccca tttgctattg aacttatgga agcaaaagga
    10321 gttgcttatg ttcaacatga tgttccagag gtaacactag ggatggaaga gtaaagagga
    10381 agatagagca tatgcctatc ttccttgaag gttttatcgt ttaagctaag ggccagttat
    10441 gttaaatata ttgaacgaga taattctcaa aattggtcaa ttgttagtcg tgggattact
    10501 gaaaatggcc taaaaaaggt gatcaccagt taatagtcat tattcctaat gatttttcac
    10561 agaaagtttt agatgttgat aatagaatag ctgatccatc tattatcaac atctaaaatt
    10621 agtactgctg ggaatctcga attgcaaaaa taatttacaa aaaagagaaa agatattgtt
    10681 gctgacctca atagatgtaa atccagttta tgctaatatg gcatttgatt gggtgtatga
    10741 taactataaa gatgatatca tcaatatgga caaggaacta tcaaaaatat tggtaagtcc
    10801 atttcagcta ataatagttt aggtacttta tataattaag gagcaagatt tgaaaacgga
    10861 aatagaaaca aataatctta ttaatttagg atggtttcat gataatgctg tttattatga
    10921 tatgaaaacg aaaaaattat ataaatctaa tctaaaa
//
LOCUS       DATRNK010000062        10621 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.062, whole genome shotgun sequence.
ACCESSION   DATRNK010000062 DATRNK010000000
VERSION     DATRNK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10621
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.062"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            41..2278
                     /gene="metE"
                     /locus_tag="VBC52_001663"
     CDS             41..2278
                     /gene="metE"
                     /locus_tag="VBC52_001663"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEO6883232.1"
                     /translation="MVKVSNLGYPRLGEQREWKQAIEAFWAGNLEQKDLEKQLKQLRI
                     NHLKKQKEAGIDLIPVGDFSCYDHVLDLSFQFNIIPKRFDEYERNLDLYFAIARGDKD
                     NVASSMKKWFNTNYHYIVPEWEVETKPHLQNNYLLDLYLEAREVVGDKAKPVITGPIT
                     YVSLSSGIVDFEATVQRLLPLYKQVFQDLIDAGATYIQIDEPIFVTDEGELLVDIAKS
                     VYDFFAREVPQAHFIFQTYFESAVCLDKLSKLPVTGFGLDFIHGRAENLAAVKQGLFR
                     EKELFAGIVNGRNIWAVNLEETLALLEEIGPFVKRLTLQPSSSLLHVPVTTKYETHLD
                     PVLKNGLSFADEKLKELELLASAFDGNKTKGYHEALSRFSALQAADFRHVALESLAEV
                     KLERSPYKLRQALQAEKLQLPILPTTTIGSFPQSPEIRKKRLAWKRGNLSDSDYKDFI
                     KTEIRRWIAIQEDLDLDVLVHGEFERVDMVEFFGQKLAGFTTTKLGWVQSYGSRAVKP
                     PIIYGDVKHIQPLSLEETVYAQSLTKKPVKGMLTGPITITNWSFERDDISRSDLFNQI
                     ALAIKDEIQLLEQSGIAIIQVDEAALREGLPLRQQKQQAYLDDAVAAFKIATSSVKDE
                     TQIHTHMCYSKFDEIIDSIRALDADVISIETSRSHGDIIESFETAVYPLGIGLGVYDI
                     HSPRIPTKEEIIVNIQRSLKCLSKEQFWVNPDCGLKTRREAETIAALEVLVSATKEVR
                     QQLDN"
     gene            2323..4167
                     /locus_tag="VBC52_001664"
     CDS             2323..4167
                     /locus_tag="VBC52_001664"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEO6883233.1"
                     /translation="MSKFLEKLKTDILVADGAMGTLLYTYGLDTCHESYNVTHPEKVL
                     AIHQAYIEAGADVIQTNTYGAQRHRLKNYGLEDQVVSINQAAVNLAHQATLGKETFIL
                     GTVGGFRSQRQCDLTLDNIVEETLEQVEALLATGQLDGLLFETYYDIEEITTVLKIVR
                     EMTDLPIITNISLHEAGVTSNGKPIVEALSQLVMLGADVIGLNCHLGPYHMIQSLKQV
                     PLFAQSYLSVYPNASQLSLDGENSQYQFSQNSEYFGKSAELLVAEGVRLIGGCCGTTP
                     DHIRAVKRSIRGLKPIERKVVTPIIPVKDFVRRIRRTDTLVDKVKKEVTIIAELDPPK
                     HLDIVQFQKAIRAIDQKGIAAITLADNSLSNTRICNLSIASLLKDEISTPFLLHIACR
                     DHNLIGLQSRLLGMELLGFNHILAITGDPTKLGDFPGATSVYDVTSFKLLSLIKQLNQ
                     GLSYSGASLRRPTDFTVAAAFNPNVKNLTRTVKLIEKKVASGADYFMTQPIFDHSVLK
                     ELADLTKTVEQPFFIGIMPITSYNNAVFLHNEVPGIKLSESFLSALEKVKDDKEACLT
                     LALNESKSLIDEALNYFNGIYLITPFLRYDLTLELIDYIQKKQVRKSS"
     gene            4514..5221
                     /locus_tag="VBC52_001665"
     CDS             4514..5221
                     /locus_tag="VBC52_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEO6883234.1"
                     /translation="MVCVDFIDQKVYSEAEGLFVSDLDLGIKGLIRARYPFALDSDFI
                     SYGNLSHYCMNYLDEIVQRANQKNEKIKYNVTTLMKEEEKPFNVEERLNKQATIGQRI
                     ADDVARFGGSWTFIIVFVSIMAIWMLVNIMKPFGIQFDPYPFILLNLALSTIAAIQAP
                     LIMMSQNRAADYDRLQARNDFNVNKTSELEIRLLHEKIDHMVQQDQFELLEIQKLQTE
                     MLVSLGNQLAQLKQLQK"
     gene            complement(5386..6471)
                     /locus_tag="VBC52_001666"
     CDS             complement(5386..6471)
                     /locus_tag="VBC52_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO6883235.1"
                     /translation="MTTNVMANYSYGEDCFKEIPSVLATYRIQSIAFVGGKRALDSSE
                     AEVRSILEEANIKVTGSFVYGTDSTQSNIDKLVANPQVQAADAILGFGGGKALDTAKM
                     VAKELGKNSFTIPTICSNCSAGTAIAVVYNDDHSFLRYGYPESPLHIFINTRIIAQAP
                     SKYFWAGIGDGISKAPEVERATLEAKTNKLPHTAVLGQAVALSSKEAFYQFGEQGLKD
                     VEANLASRAVEEIALDILISTGYASNLVNQPDFYYNSCHAHAFYYGTTAIQRQGEFLH
                     GVVVAFGVLVLHAYFNELEELEKVARFNKSLGLPTTLADVSLSEKDIPKIVEIAMTTN
                     EYKNTPFDPKMFAQAILAADAFGQTLA"
     gene            complement(6672..7211)
                     /locus_tag="VBC52_001667"
     CDS             complement(6672..7211)
                     /locus_tag="VBC52_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEO6883236.1"
                     /translation="MKIAIIGYSGSGKSTLARKLGNYYNCNVLHLDSIHFAPNWEERK
                     YDDMIDDVSSMLERRTWIIEGNYKKLLYQERLADADEIIFFDFNRFNCLWRAFKRYCK
                     FRGKTRPDMANGCPEKLDFEFISWILKDGRSDKQKSNYKQVIENYPQKIKILKHQRDL
                     EQYLKELPVASNTDPLKSL"
     gene            7980..8747
                     /gene="cfb"
                     /locus_tag="VBC52_001668"
     CDS             7980..8747
                     /gene="cfb"
                     /locus_tag="VBC52_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfb"
                     /protein_id="HEO6883237.1"
                     /translation="MNVKHMMYLSGTLVAGALLFSPAVLEVHADQVTTPQVVNHVNSN
                     NQAQQMAQKLDQDSIQLRNIKDNVQGTDYEKPVNEAITSVEKLKTSLRANPETVYDLN
                     SIGSRVEALTDVIEAITFSTQHLANKVSQANIDMGFGITKLVIRILDPFASVDSIKAQ
                     VNDVKALEQKVLTYPDLKPTDRATIYTKSKLDKEIWNTRFTRDKKVLNVKEFKVYNTL
                     NKAITHAVGVQLNPNVTVQQVDQEIVTLQAALQTALK"
     gene            8920..9222
                     /locus_tag="VBC52_001669"
     CDS             8920..9222
                     /locus_tag="VBC52_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO6883238.1"
                     /translation="MTKKITVVALETLIAQHNNIHLIDVREEHEYRGGHIPGAINLPL
                     SQLSHKFEQLDKNKEYYLVCQRGGRSIRACQFLELKGYKVINVDGGTEAWRGNLEV"
     gene            complement(9379..10242)
                     /locus_tag="VBC52_001670"
     CDS             complement(9379..10242)
                     /locus_tag="VBC52_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6883239.1"
                     /translation="MNQEKKRLNLIGLILLAQAIVLSLATTLFAEILQNDVWIGIAST
                     LIALLIPCFFMGFKNLKKHLTATHHTITFTTILFLLSIIYTTNNIVSTIWDAVLNHLN
                     IMESESQNYDATFSILMISYSVVVGPFFEEVLYRGIVLNLLSKYGKWFAIITSGILFG
                     LMHQDISQLLTTSIAGIIMGFIAYHYSFKVALLLHICNNFIVEIFTQLSTVNELYGTY
                     FENILLILAILFILYYLFTHRNTARHRISLHSNVREADSINSKQYTKLLLTSWPFILL
                     VIYDIVLTTIN"
     gene            complement(10547..>10621)
                     /locus_tag="VBC52_001671"
     CDS             complement(10547..>10621)
                     /locus_tag="VBC52_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEO6883240.1"
                     /translation="LFSDDIAEIEDFITNYKVPSTRQY"
BASE COUNT         3548 a         1653 c         2013 g         3407 t
ORIGIN      
        1 aaaagggtta gttattagct ataacttgat ggaggaaatt atggtaaaag tttcaaattt
       61 agggtatcca cgtcttggtg aacagcgcga atggaagcaa gcgatcgaag ctttctgggc
      121 agggaatctt gaacaaaaag atttagaaaa acaactaaaa caattacgta tcaatcattt
      181 aaagaaacaa aaagaggcag gtattgacct tattccagtg ggggattttt cttgttatga
      241 tcatgttttg gatttgtcat ttcaattcaa tataatccca aagcgtttcg atgagtatga
      301 gaggaattta gacctttatt ttgctattgc aagaggtgac aaagataatg tcgcatcatc
      361 tatgaaaaag tggtttaata ccaactacca ctacatagtc ccagaatggg aggttgagac
      421 taaacctcac ttgcagaata attacttact tgatctttat ctagaagcta gggaagtagt
      481 tggtgataaa gcaaagccgg ttatcactgg tccaataacc tatgtttcct tatcatcagg
      541 aattgtcgac tttgaagcga ctgttcagcg gttattacca ctttataagc aggtctttca
      601 agatttgata gatgcaggcg ccacctatat tcagattgat gagccgatat ttgtaactga
      661 tgaaggtgaa cttttagtag atatagctaa gtctgtctat gatttttttg caagagaagt
      721 accacaagcc cacttcatct ttcaaaccta ctttgaatca gcagtctgtt tagataaact
      781 ctctaagctg ccagtaacgg gatttggcct tgattttata catggtaggg cggaaaattt
      841 agctgctgtt aagcaaggtc tattccgcga aaaagaatta tttgcaggaa ttgttaatgg
      901 tcgaaatatc tgggcagtaa atttagaaga aacgttggct ttattggaag agataggtcc
      961 ctttgttaaa cgtttgaccc ttcaaccttc ttcaagtctt ttacatgtac cggtgacgac
     1021 taaatacgaa acacatttag accctgtgtt aaagaatggc ttatcatttg ctgatgaaaa
     1081 actaaaggaa ttagaactat tagctagtgc ttttgatggt aataaaacaa agggatatca
     1141 cgaagcttta tctcgttttt cagctcttca agctgctgat tttcgtcatg tagcattgga
     1201 atcattagca gaagtaaagc ttgaacgaag tccgtataaa ttacgccaag ctttgcaagc
     1261 tgaaaaatta cagttaccga ttttaccaac aacaactatt gggtcctttc ctcaatcacc
     1321 tgaaattagg aagaaacgcc ttgcttggaa aagaggaaat ctatctgact cagattataa
     1381 agatttcata aaaactgaaa ttagaagatg gattgctatt caagaagatc ttgatcttga
     1441 tgtgttagta catggcgaat ttgagcgtgt ggatatggtt gaattttttg gtcaaaagtt
     1501 agctggtttt acgacaacca aattaggctg ggtacagtct tatggttcaa gggcggtcaa
     1561 accacctatc atttatggtg atgtcaaaca tattcaaccc ttaagccttg aagaaacggt
     1621 ttatgcccaa agcttgacta agaaacctgt taaaggcatg ttgacaggtc ctattactat
     1681 aacgaactgg tcatttgagc gagatgatat tagccgatct gatcttttta atcaaattgc
     1741 tttggctata aaagatgaga ttcaactttt ggaacaatca ggcattgcta ttatacaagt
     1801 ggatgaagca gcccttcgag aaggtttacc cttacgccag caaaagcaac aggcttactt
     1861 agatgatgct gttgcggcct ttaaaattgc aacttcatct gtgaaagatg agacacaaat
     1921 tcatacacat atgtgttatt caaaatttga cgaaattatt gattctatcc gtgcactaga
     1981 tgcagatgtt atttctattg aaacgagtag aagtcatggg gacatcattg aaagttttga
     2041 aacagcagtt tatcctctag gaattggcct gggtgtttac gatattcatt cccctcgcat
     2101 acctactaag gaagaaatta ttgtcaatat tcaacgatca ctaaaatgtc tatcaaaaga
     2161 gcaattttgg gtaaaccctg actgtggctt aaaaacacgt cgtgaagcag aaacaattgc
     2221 tgccttggag gttcttgttt cagctaccaa agaggttcgt cagcaattag ataattaata
     2281 ggttaatcag taaaggattg ccatgaggga ggagtaaaga agatgtccaa gtttttagag
     2341 aaattaaaaa cagatattct agtagctgac ggtgcaatgg gaactctcct ttatacttat
     2401 gggctagata cttgtcatga atcctacaat gtaacacatc cagaaaaggt tctagccatt
     2461 catcaagctt atattgaagc gggtgcagat gttattcaga cgaataccta cggggctcaa
     2521 cgtcatcgat taaagaacta cggtttggaa gatcaggttg tctctatcaa tcaagcagca
     2581 gttaatctag ctcatcaagc gactttgggt aaagaaacat ttattttagg tactgttgga
     2641 ggttttcgtt ctcaaagaca atgcgattta acattggata atattgtaga agaaactttg
     2701 gaacaagttg aggctttatt ggcgactgga cagctagacg gattattgtt tgaaacttat
     2761 tatgatattg aggagataac gacagtttta aaaattgtga gggagatgac tgatctacca
     2821 attattacta atatttctct tcatgaagca ggggtaacat caaatggaaa gcctattgta
     2881 gaggcgctca gccaattagt tatgttaggt gcagatgtga ttggcttaaa ttgtcacctt
     2941 ggaccctatc atatgatcca atcattgaag caagttcctt tatttgccca gtcttatttg
     3001 tcagtttatc ctaatgcaag tcagttatct ttagacggtg agaatagtca ataccagttt
     3061 agtcagaatt cagagtattt tggaaaatct gcagaacttc tcgtagcaga aggagttagg
     3121 cttattggtg gttgttgtgg gacaactcca gatcatatta gagctgtgaa acgatctatt
     3181 agaggattga aacctattga acgaaaggtt gttactccta taatacctgt aaaagatttt
     3241 gttaggcgta tcagacgaac agatactcta gtggataagg taaaaaaaga agtaacaatt
     3301 atcgcagaat tagatcctcc aaaacattta gatatcgttc agtttcaaaa ggctatacga
     3361 gcgattgacc aaaaaggtat tgcagcaatt actttagcag ataattcact atctaataca
     3421 cgtatttgta atttaagtat agctagcttg cttaaagatg aaattagtac acccttttta
     3481 ctacatatag cttgtcgtga ccataattta attggcttac agtctagact gttgggcatg
     3541 gaattattag gttttaacca tattttagcc ataacaggag atccgactaa acttggtgat
     3601 ttcccaggag cgactagtgt ttatgatgtg acttccttta aactcctttc tctgattaaa
     3661 caacttaatc aaggtttatc ttatagtggt gctagcttga gacgtccaac cgattttaca
     3721 gttgctgctg cctttaatcc taatgtgaaa aaccttactc gaaccgtaaa attgattgag
     3781 aaaaaagtag cttctggtgc tgattatttt atgacacaac caatatttga tcactctgtg
     3841 ttaaaggaat tagctgatct tactaaaaca gttgaacaac ccttttttat cggaattatg
     3901 ccaataacaa gttataataa cgctgttttt ctacacaatg aagttcctgg tattaaactt
     3961 tctgagtcat ttttatctgc tcttgaaaag gtaaaggacg acaaagaagc ttgtttaact
     4021 ttggccttaa acgagagtaa gtccttgatt gatgaagcct tgaactattt taatggtatc
     4081 tatttgatta ctcccttttt acgctatgat ctgactcttg agttaattga ctatatacaa
     4141 aaaaagcaag taagaaagtc ctcttaactt tcttactttt tagttggtcg tttattatta
     4201 taataaaagt tacgtaccaa aacagaggtt aaactagatt tttaagccac cttctatact
     4261 aagataaatt agttaattta agttttcttt aagtttatag acttagaata aagcgttatt
     4321 ttgatgggta gcataaaatc aaggcataat atagatgtca gcgatttttt tagactaacg
     4381 aaataacgat gtaaagctta ataggggtgt atcaaatact cacttatata gtaccaaaag
     4441 cctatagcct gttggctagc ttgaccgtat tcaaatattg aataaatctt ggttgagaaa
     4501 gggaggtata aaaatggtat gtgtcgattt tattgatcaa aaagtttata gtgaagcaga
     4561 aggtctattt gtttccgatt tagacttagg cattaaaggt ttaataagag cacgttatcc
     4621 ttttgctctg gatagtgact ttatttcgta tggaaattta agtcattatt gtatgaatta
     4681 tcttgacgaa attgttcagc gagcgaatca gaaaaatgag aagataaaat ataatgtgac
     4741 taccttgatg aaagaagaag agaagccctt caacgttgaa gagcgtttga acaagcaagc
     4801 aactatcggt caacgaattg ctgatgatgt tgcgcgtttt ggcggatctt ggacttttat
     4861 tatcgttttt gtatccatta tggctatttg gatgttggtt aatatcatga agccttttgg
     4921 tattcaattt gatccttacc catttatcct gttaaactta gctttatcaa cgattgctgc
     4981 aatccaagca ccacttatta tgatgagtca aaatcgtgct gctgattatg atcgtttgca
     5041 agctcgaaat gactttaatg tcaataaaac ctctgagtta gaaattagat tattgcacga
     5101 aaagattgac catatggtac aacaagatca gtttgaattg ttagagattc aaaagttaca
     5161 gacagaaatg ctagtatcat tgggaaatca gttagcacaa ttgaaacaac tacaaaaata
     5221 atcttttagc agttatttaa aaatactgat acaatactaa gaatttaaat taatactgca
     5281 acttcctata atggagagca gtatttttac tttattaata acaaaaaccc cttagatgat
     5341 gtgtctaagg gatttttgta tcggaataat cgagttggtg aattattagg ctagtgtctg
     5401 tccgaaagcg tctgctgcta aaatagcttg agcaaacatt ttgggatcga aaggggtatt
     5461 tttgtattca ttagtagtca ttgcaatttc aacaattttt ggaatatctt tttctgataa
     5521 agagacgtca gctagagttg ttggcaaacc tagtgactta ttaaaacgag ctactttttc
     5581 aagttcttct agttcgttga aataagcatg taaaactaac acaccaaacg caactacaac
     5641 gccatgtaag aattcacctt gtctttgaat agcggttgta ccatagtaaa atgcatgcgc
     5701 gtgacaagaa ttgtagtaga aatcaggttg attaactaaa ttagaagcat agccagttga
     5761 gattaagata tcaagcgcaa tttcttcaac tgcacgcgaa gctaaattag cttcaacgtc
     5821 ttttagacct tgttcaccaa attgataaaa agcttccttt gaagacagag cgactgcttg
     5881 tcctaacact gcagtatgtg gtagtttatt ggtcttagcc tctaaggtag cacgttctac
     5941 ttcaggggct tttgaaatac cgtccccaat accagcccaa aaatatttgc ttggtgcctg
     6001 tgcaatgatc cgtgtattga taaaaatatg aagtggagac tcgggatagc cgtatcttaa
     6061 gaaagaatgg tcatcattat atacaactgc aatggcagta cctgcggagc aattagaaca
     6121 aatagttggg atagtaaagc tatttttacc taactctttt gctaccattt tagctgtgtc
     6181 taaagctttt ccaccgccaa aacctaagat agcatcagca gcttgaactt gtgggttggc
     6241 tactaacttg tcaatgttag attgtgttga atccgttcca tagacaaagc ttcctgtgac
     6301 tttaatattt gcttcctcta aaatagagcg aacctcggct tctgagctgt ctagggcacg
     6361 tttgccaccg acgaaagcaa tgctttgaat acggtaagtt gccaaaacgc taggaatttc
     6421 tttgaagcaa tcttctccgt agctataatt tgccattaca tttgttgtca ttcagtgtcc
     6481 tcctctattc caaaggtcga ttagagacag ctattttcta aaagtgaata gttttttgtc
     6541 tcttgaccaa gtgattatca tattatagca gaatattcag aaaattggaa catgaaaaac
     6601 aatttaattt tcaagaataa gaatagaaaa ggtaaagcga gatatacgag taaatactgt
     6661 cagtataata attataagct cttgagaggg tctgtattgg atgcaacagg gagttctttt
     6721 aggtattgct caagatcgcg ttggtgtttt agaatcttta ttttttgagg gtagttctca
     6781 ataacttgct tataatttga tttttgttta tcagagcgac cgtctttaag aatccaactg
     6841 ataaattcaa aatctaattt ttccgggcat ccattggcca tatcaggtcg agtcttacca
     6901 cgaaacttac agtaacgttt gaatgctctc cacaagcagt tgaagcgatt aaagtcaaaa
     6961 aaaataatct catctgcatc tgctagacgt tcttgatata gcagtttttt gtagttacct
     7021 tctataatcc atgttcttct ttctaacatg ctgctgacat cgtcaatcat gtcatcatat
     7081 tttctctctt cccaatttgg ggcgaagtga atactatcaa ggtgtaatac attgcaatta
     7141 tagtaatttc ccaattttct cgctaaagtg gacttacccg acccactata tccgattatt
     7201 gctattttca tatactacct ccgttaaatt gtttacttga agttaatgct gtacttagga
     7261 tttacttatt ttataagggt tattcacttt tttagattaa tatatttttg atataagtta
     7321 tccatacaca tggtaacata attaccagta aatgtaatct agatatcaat actatggttg
     7381 acgatattgt taacttttgg ttcaaaaaat tgggtaaaaa tcatcggagg gaactgccaa
     7441 acaaactcac agtttaaaat actaagtaat aaaaagtctt gaacttatta tgtttaaggc
     7501 tttttaagcg tctaaactac ttggtctagt atgaatttct aaacagaaag taaaaataat
     7561 gagttttagt atggtttttt gggcataatg gaacgatttt atctaaaata gtacgtttct
     7621 tattaaattt attttttaag aaaaacatta ggctaatcta tttgataaaa atagtgataa
     7681 gtagaaattt tctaaaatat ttagatttat ataaggtggt aaattctcgt tatggttttt
     7741 tacatgaaaa attaaattaa attatcagaa attaacaaaa ggataggcgc aagtatatta
     7801 tagcaaaatg ataatattag ttttatgaaa aaaatgcagt ttatttgtaa taatgtaaaa
     7861 tataggggaa aagaaagcgc ttgacgacct tttggacaag tagtaagata caaacatggg
     7921 ccctgtaaat taaaaatact gcagtaaaag tgattttagt ttaaaggagg aaattcatta
     7981 tgaacgttaa acatatgatg tatctatctg gaactctagt ggctggtgca ttgttatttt
     8041 caccagctgt attagaagta catgctgatc aagtgacaac tccacaagtg gtaaatcatg
     8101 taaatagtaa taatcaagcc cagcaaatgg ctcaaaagct tgatcaagat agcattcagt
     8161 tgagaaatat caaagataat gttcagggaa cagattatga aaaaccggtt aatgaggcta
     8221 ttactagtgt tgaaaaatta aagacttcat tgcgtgccaa ccctgagaca gtttatgatt
     8281 tgaattctat tggtagtcgt gtagaagcct taacagatgt gattgaagca atcacttttt
     8341 caactcaaca tttagcaaat aaggttagtc aagcaaatat tgatatggga tttgggataa
     8401 ctaagctagt tattcgcatt ttagatccat ttgcttcagt tgattcaatt aaagctcaag
     8461 ttaacgatgt aaaggcatta gaacaaaagg ttttaactta tcctgattta aaaccaactg
     8521 atagagctac catctataca aaatcaaaac ttgataagga aatctggaat acacgcttta
     8581 ctagagataa aaaagtactt aacgtcaaag aatttaaagt ttacaatact ttaaataaag
     8641 caatcacaca tgctgttgga gttcagttga atccaaatgt tacggtacaa caagttgatc
     8701 aagagattgt aacattacaa gcagcacttc aaacagcatt aaaataatat ttgcattttt
     8761 cgtgtgatgc tgtcgacttc gtgattttgt actaccatga tttttacgat taaaaggttt
     8821 acgataatag tcataatagt agaacgatgt caccatttta aataataaag tgattagtca
     8881 tttgactaaa tttaccaagt atcaaaggaa ataaagatta tgactaaaaa gataactgtt
     8941 gtagcattag aaacattgat tgcccagcat aataatatcc atttgataga cgttcgtgaa
     9001 gagcatgagt atcgtggggg gcatattcca ggtgcgataa atcttccttt gagtcaactc
     9061 agtcataagt ttgaacagtt agataaaaat aaggaatatt atcttgtttg tcaacgaggg
     9121 ggaagatcta ttagagcatg ccaatttctt gagttgaaag gctacaaagt aatcaatgta
     9181 gatggtggca cagaagcgtg gagaggaaat ctagaagtgt aaatatgtcc tgtgtagtct
     9241 accatatttg ataataaaaa aattgacatt atctcactat taatgacctt ttagtaaaca
     9301 taaagaaaag tatgtgtttt gaaaaggagg atgattatcc ttttcaaaac catacttttt
     9361 gtgtcttgtg gcttggtctt aattaatagt tgttaggaca atgtcgtaaa tgacaaggag
     9421 aataaaaggc caactagtaa gtaataattt tgtgtattgt ttagagttta ttgagtcagc
     9481 ctctcgaacg ttagagtgca gacttatacg atggcgagca gtattcctat gcgtaaaaag
     9541 gtaatataat ataaataaga ttgctaaaat tagcaatata ttttcaaaat aagtcccgta
     9601 taactcgttt actgttgata gctgggtgaa aatttctacg ataaagttat tgcagatatg
     9661 aaggagtagg gctaccttaa aggaataatg gtaagcgata aaacccatta tgatacccgc
     9721 aatagatgta gtcaatagct gagaaatgtc ttggtgcatt aaaccaaata ggatgccaga
     9781 agtaataatg gcaaaccatt taccatactt ggataataaa ttcaatacta ttcctcggta
     9841 taaaacttct tcaaaaaaag gacctacaac aacagaataa ctaatcataa gtatactgaa
     9901 ggtggcatca taattttgac tttccgattc cataatattg agatgattta gcacagcatc
     9961 ccatattgtg gagactatat tattagtagt atatataatg cttaacaaga ataaaatagt
    10021 ggtgaaagtg attgtatgat gagttgccgt tagatgtttt tttaaatttt taaaacccat
    10081 aaaaaaacaa ggaatgagta aggcgattaa agtacttgct atacctatcc agacatcatt
    10141 ttgtaagatt tcagcaaata gtgtagtcgc caaacttaaa acaatagcct gagctaaaag
    10201 tatcagcccg attaggttaa gtctcttttt ttcttgattc aattgattac ctccttatta
    10261 ataacggtaa cgacaaagaa atattataat gtataatgta taggtttcta agtcattagt
    10321 caatagggac gggcacttgg atacttctaa taagataaaa tacatagaca agactatggt
    10381 tttggcttct ctaaagatta cctaaaaatt tataagttct cgatcagaaa ggatgagttt
    10441 ggatattgag aggaggggct tgaccagctg aaagaaaaat gaaccaaggg taatcgcttc
    10501 gaaaaaagaa ggaaatttca attagaaact tccttttttt atcaatctag tattggcgcg
    10561 tgctagggac tttataattc gttataaaat cttcgatttc agcaatatca tctgagaata
    10621 g
//
LOCUS       DATRNK010000063        10524 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.063, whole genome shotgun sequence.
ACCESSION   DATRNK010000063 DATRNK010000000
VERSION     DATRNK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10524
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.063"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            147..2216
                     /locus_tag="VBC52_001672"
                     /pseudo
     CDS             147..2216
                     /locus_tag="VBC52_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771255.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
     gene            complement(2288..2358)
                     /locus_tag="VBC52_001673"
     tRNA            complement(2288..2358)
                     /locus_tag="VBC52_001673"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2324..2326),aa:Cys,seq:gca)
     gene            complement(2493..4025)
                     /gene="ahpF"
                     /locus_tag="VBC52_001674"
     CDS             complement(2493..4025)
                     /gene="ahpF"
                     /locus_tag="VBC52_001674"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEO6883241.1"
                     /translation="MVLDKEIKAQLAQYLDLLESDIVLQADLGDNDNSQKVKDFLDEI
                     VAMSDRISLESTHLKRQPSFGIAKKGHESRVIFSGLPMGHEFTSFILALLQVSGRAPK
                     VDEDIIKRIKGIEKTINLETYVSLTCHNCPDVVQAFNIMAVLNPNITHTMIEGGMYQD
                     EVKSKGIMSVPTVYKDQEEFTSGRATIEQLLEQLDGPLDAEAFADKGVYDVLVIGGGP
                     AGNSAAIYAARKGLKTGILAETFGGQVIETVGIENMIGTLYTEGPKLMAQIEEHTKSY
                     DIDIIKSQLATGIEKKELVEVTLANGAILQAKTAILALGAKWRNINVPGEEEFRNKGV
                     TYCPHCDGPLFEGKDVAVIGGGNSGMEAALDLAGVTKHVTVLEFLPELKADQVLQERA
                     AKTDNLTILKNVATKDIVGEDHVTGLNYTDRDTNEEKHIDLEGVFVQIGLVPSTSWLK
                     DSGIELNERQEIVVDKFGSTNIPGIFAAGDCTDAAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(4043..4603)
                     /gene="ahpC"
                     /locus_tag="VBC52_001675"
     CDS             complement(4043..4603)
                     /gene="ahpC"
                     /locus_tag="VBC52_001675"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEO6883242.1"
                     /translation="MSLVGKEIIEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLDVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQGFDVLGQDGLAQRGTFIIDPDGVIQMMEINADGIGRDASTLIDKVRAAQYIRQH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            4880..5650
                     /gene="rpsB"
                     /locus_tag="VBC52_001676"
     CDS             4880..5650
                     /gene="rpsB"
                     /locus_tag="VBC52_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEO6883243.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVAEEAKRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFELLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDVIIPANDDAIRAVKLITSKLA
                     DAVIEGRQGEDADVDFAQEAQADSIEEIVEVVEGSKND"
     gene            5744..6784
                     /locus_tag="VBC52_001677"
     CDS             5744..6784
                     /locus_tag="VBC52_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEO6883244.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDLDKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVDGNVAAVIEVNAETDFVAKNDQFVTLVNETAKVIAE
                     GRPSNNEEALALTMPSGETLEQAFVTATATIGEKISFRRFALVEKTDEQHFGAYQHNG
                     GRIGVITVVEGGDDALAKQVSMHVAAMKPTVLSYTELDAQFVHDELAQLNHKIEQDNE
                     SRAMVNKPALPFLKYGSKAQLTDEVIAQAEEDIKAELAAEGKPEKIWDKIVPGKMDRF
                     MLDNTKVDQEYTLLAQVYIMDDSKTVEAYLESVNAKAVAFVRFEVGEGIEKASNDFEA
                     EVAATMAAALEK"
     gene            complement(6944..7405)
                     /locus_tag="VBC52_001678"
     CDS             complement(6944..7405)
                     /locus_tag="VBC52_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HEO6883245.1"
                     /translation="MNYIITFLISMIPLVELRGAVPFAIANGIPLWEALAIGVVGNML
                     PVPIIFFFARKVLEWGADKPYTGKFFTWCLKKGHSGGQKLEKVAGEKGLFIALLLFVG
                     IPLPGTGAWTGTLAASLLDWEFKHSVIAVMLGVILAGCIMGTLSIIGFNLF"
     gene            7578..8042
                     /locus_tag="VBC52_001679"
     CDS             7578..8042
                     /locus_tag="VBC52_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEO6883246.1"
                     /translation="MAIKNTSDNIEEYIKSLLEQSGIAEIKRSNLADTFQVVPSQINY
                     VIKTRFTESRGYVVESKRGGGGYIRIAKVHFSDQHQLFGNMLSTIGERISEQVFDDLI
                     QLLFDEEIITEREGNLILATSGDDVLGEQASVIRARMLRKLLQRLDRKGFHL"
     gene            8039..10486
                     /locus_tag="VBC52_001680"
     CDS             8039..10486
                     /locus_tag="VBC52_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO6883247.1"
                     /translation="MSHYSIKLQEVFRLAQFQAARYESHYLESWHLLLAMVLVHDSVA
                     GLTFAEYESEVAIEEYEAATILALGRAPKEEITDYQFLEQSSALKKILKLAENISIVV
                     GAEDVGTEHVLLAMLVNKDLLATRILELVGFRGQDDGESVRMVDLRKALERHAGFTKD
                     DIKAIYELRNPKKAKSGASFSDMMKPPSTAGDLADFTRDLSQMAVDGEIEPVIGRDTE
                     ISRMVQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGNIPYELRDMRVLELDMMS
                     VVAGTRFRGDFEERMNQIIADIEEDGHIILFIDELHTIMGSGSGIDSTLDAANILKPA
                     LARGTLRTVGATTQEEYQKHIEKDAALSRRFAKVLVEEPNLEDAYEILLGLKPAYEAF
                     HNVTISDEAVMTAVKVAHRYLTSKNLPDSAIDLLDEASATVQMMIKKNAPSLLTEVDQ
                     AILDDDMKSASKALKASHKDKKRKPIAVTEDHIMATLSRLSGIPVEKLTQADSKKYLN
                     LEKELHKRVIGQDDAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKALA
                     EVLFDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTEKVRNKPYSVLLFD
                     EVEKAHPDIFNVLLQVLDDGVLTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFGA
                     KDISHDYTAMQKRIMEELKKAYRPEFINRIDEKVVFHSLSQDNMREVVKIMVKPLILA
                     LKDKGMDLKFQPSALKHLAEDGYDIEMGARPLRRTIQTQVEDHLSELLLANQVKEGQV
                     IKIGVSKGKLKFDIAKS"
BASE COUNT         3355 a         1796 c         2053 g         3320 t
ORIGIN      
        1 taaagtaaag aagaaaaaga agaaattaat aatacttgaa aaagaaagat gtcgctgtcg
       61 attggagaga gaagatacta aaaaaatcga tgataaaatt gagaaattga agaaagaatt
      121 gatagatgtt atgaaggagg aataatatgg aagaaactat tctaatagtc agctttctcc
      181 tgtttttgat tttatctaat gttatcaata ggatatttcc taaattacca ttaccattta
      241 tccagttggt ctttgggatt ctctctggct tagtttttca taagtcacaa gtccatatcg
      301 atcctgagtt atttcttgcc tttgttattg cccctttgaa ctttagagaa ggtcaagaaa
      361 gtgatattgg tagttttata aagtatcgga ctattattct ttacttaatc ttaccaacgg
      421 tatttttaac agctattgtc gttggttatg tagcagggca ccttttgcca gttagtttac
      481 cacttgcggc atgctttgcg cttggagcag ccttaggtcc aacagatgcc gttgccttta
      541 tatcgattgc taagaggttt caatttccta aacgagcaga gaatatcctc aaattggaag
      601 gtcttttaaa tgacgctagt ggtctagttt ccttccagtt tgctttaaca gcccttttaa
      661 caggttattt ttccttaaca aaagctagtc tcaagttagc attggccata atgggtggtt
      721 ttttaattgg cttgcttttt gcttttctaa cgaggttatg tctcactgtc ctagagaaat
      781 ttgatgctgc tgatgttaca ggggctctac ttttggaatt aaccttacct tttgttgctt
      841 atttcgttgc tgatttgttg ggattttcag ctattattgc ggtggtagtg gcaggagtaa
      901 tgcaagcaaa tcgtttgaaa aaagtgactc tctttgatgc tcaagtagat cgcgttacct
      961 ctgttatttg ggaaacctta aattttattt taaatggttt agttttctta atatttggaa
     1021 gagaactgac acgtatcatt ggtcctcttt taacaagtaa cgcttatagt aattttgatc
     1081 ttattagtat tgttgtttta gttacttgca cactttttct agttcgcttt ctagcagttt
     1141 cttgctttta tgcttggaga tctttcaaat accataaatc atttaaaaag tattggagag
     1201 agattcaact gttgactttt tcaggagtta aaggaagtgt ttccatagca acaatactct
     1261 tattacccaa gcactcagtc ataggagagt tggggtatag tttgattctt ttcactgttg
     1321 gtgcagtaac actaatgagt tttttaacgg gacttttagt attaccaaag ttagcgcctc
     1381 ccttacaggt gaaagatgat tatttaatac gtctatcgat tttaacaaaa gtattatctg
     1441 tcttagaaga agacggtaag atttctgaaa atcaagcttc attttatgca gttatagata
     1501 attataatag ccgtattcgt catctcattt tagaacaaga atctagtgac attaagaagg
     1561 acttagctga attgcaactg atgatgctta gtatagaaag cgacgggctt gaagctgctt
     1621 atcgttatgg caatatttct atcaaagaat atcgtattta ctagcgttat ttaaaatact
     1681 tagaacgtca agttaatcgt agctttgtgt caaatatcac ttatagttta acgattctta
     1741 tgcgtgttat tcgtggtatt gttcatgagt ttttaacatt tggacaagga atacgaaatc
     1801 ggaaaaaaca tttcaaaaat aggaaacggt tgctagcaga agtcaatcgt cagcacttaa
     1861 cagatttgta cttatcaaat aatgatctca ttttagaaac gctagaagat atggaaggtt
     1921 attataatgc aactttgata gatttcttac aaaatgaacg cattcgagac gcagaattaa
     1981 ttaaatcagg tttatttgta gaacgtgtca ttgcacgcat aacgccagat attttaaatg
     2041 aaaccctaag aggatattac ttggaacgca gaacaatctc tgaatttgaa agggacggtt
     2101 taattagttc taaagagtct caaattttaa gggataatgt caatgaatta gaaagctatt
     2161 cattaagaga tagtcaatct tctatacctt atcaattatt ggatttaaaa aaataattct
     2221 gataacaaaa aaatcaacga acaatcgttg attttttgtt atctttggat aaaatctttt
     2281 atatttaagg cggtagacgg atttgaaccg acgatcaagc ttttgcagag ccgtgcctta
     2341 ccacttggct ataccgccat aacagataat attctatcga aaaaaaaaag attcgtcaag
     2401 cctctttcgt aaaatagcca attttagccc ttaaacttcc tcatattacc ccaaaaaaga
     2461 gattgatacc tattgtatca atctctttta gattattgtc tgattaaata atcaaatgcg
     2521 ccaatggcag ctgtggcacc agaccccatg gaaataatga tttgcttgta ggcagcatct
     2581 gtacagtcac ctgcagcaaa aataccaggg atatttgttg agccaaattt atcaacgaca
     2641 atttcttgac gttcattgag ttcaatgccg ctatccttga gccaagatgt acttggtaca
     2701 agaccaattt gtacaaaaac accttctaaa tcaatgtgtt tttcttcgtt tgtatcacga
     2761 tcggtatagt ttaaaccagt gacatgatct tcaccaacga tatctttagt agcaacattc
     2821 ttaagaatag ttaaattatc agttttagct gctcgttctt gcagtacttg gtctgctttg
     2881 agctcaggta agaattctag gactgtaaca tgtttagtga caccggctag atctaaagca
     2941 gcttccatac ctgaattacc accacctata acagcaacat cttttccttc gaaaagaggg
     3001 ccatcacaat gtggacagta agtaacacct ttattgcgga attcttcctc gccaggaacg
     3061 ttgatatttc gccacttagc acccagagct aggatagcag ttttagcttg taaaatagct
     3121 ccgttagcca gtgttacctc aactaattct tttttttcaa tgccagttgc taattgactc
     3181 ttgataatat caatatcgta agatttggta tgctcttcaa tttgagccat caatttaggg
     3241 ccttcggtgt acaatgtacc aatcatgttc tcaataccaa ctgtttcgat aacttgtcca
     3301 ccaaaagttt ctgctaaaat acctgttttt aaccctttac gggcggcata aattgcagca
     3361 ctgttacctg caggaccacc accaataact agaacatcat agacaccttt gtcagcaaat
     3421 gcttcagcat caagaggtcc atctagttgt tctaaaagct gttcaattgt agcacggcca
     3481 gaagtaaatt cttcttggtc cttataaaca gtaggaacag acatgatacc ttttgatttg
     3541 acttcgtctt gatacatgcc tccttcaatc atagtatgag tgatattcgg attcaaaaca
     3601 gccattatat taaaggcttg aacgacatct ggacaattat ggcaagttaa tgaaacataa
     3661 gtttctagat tgatagtttt ctcaatgccc ttaattcgtt tgataatgtc ttcatcaact
     3721 tttggtgcgc gaccagagac ttgcaacaaa gctaaaatga aagatgtgaa ttcatgcccc
     3781 attggcaagc ctgaaaaaat aacgcgactt tcatgccctt tttttgcaat cccaaaacta
     3841 ggttgacgtt ttaaatgtgt tgattcaagg ctgatgcgat cagacatagc gacaatttca
     3901 tctagaaaat cctttacttt ttgagaatta tcgttatctc ctaaatcagc ttgtaataca
     3961 atatcagatt ctagcaaatc taaatattgt gctaattgag ccttgatttc tttatctaag
     4021 accatggatt acctcctggt tattaaattt tacctacaag gtctaaacta ggagttaaag
     4081 tttcagctcc ttctttccat ttagctggac aaacttcacc tggatgttgg cggatgtatt
     4141 gagcagcacg gactttatca atgagagtac ttgcatcacg tccgatgcca tcagcattaa
     4201 tttccatcat ttggatgaca ccgtctggat caatgataaa tgtaccacgt tgtgctagac
     4261 catcttgtcc aaggacatca aaaccttgtg aaataaggtg tgaagggtca ccaatcatag
     4321 gatatgtgat agtaccaacg acatctgagt catcatgcca tgctttatga acaaaatgag
     4381 tatcagtaga gacagaataa acttctacat caagagattt aagtgtttcg tattgctctt
     4441 gaaggtcacc aagttcagtt gggcaaacaa atgaaaagtc tgctgggtag aaacaaaaaa
     4501 ctgcccattt tcctttaacg tcttcatttg taacagtgat gaattttcca tcgtgataag
     4561 cttgagctga aaattcaata atttcttttc cgactaatga cataataatg tcctccttta
     4621 ttttttaata gcctaattat aaacgaaata cttttaaatc tccaataaaa acgtttacat
     4681 ttttctaaga aaagtatgat ttttctaata gagtagaaga ttttatagct tgtcaaatct
     4741 aacttttttt gataaactat tatagttgct taaagtgaca aatactcatc ttagatcaat
     4801 tgtggtcttg ttgccgaaag gttaggctgc aagtcgaagt ctgagagagg aaaaaacatt
     4861 tttaaaggag aacttactca tggcagtaat ttcaatgaaa caacttcttg aggctggtgt
     4921 tcactttggt caccaaactc gtcgctggaa ccctaagatg gctaagtaca tcttcacaga
     4981 acgtaatggt atccacgtta tcgaccttca acaaactgta aaattagctg accaagctta
     5041 cgaatttgtt cgtgatgctg cagcaaacga tgcagttatc ttgtttgttg gtactaagaa
     5101 acaagcagct gaagctgttg ctgaagaagc taaacgtgct ggtcaatact tcattaacca
     5161 ccgttggtta ggtggaactc ttactaactg gggaactatc caaaaacgta ttgctcgttt
     5221 gaaagaaatc aaacgtatgg aagaagaagg aacttttgaa cttcttccta agaaagaagt
     5281 tgcattgtta aacaaacaac gtgcacgtct tgaaaaattc ttgggtggta tcgaagatat
     5341 gccacgcatc ccagatgtaa tgtatgttgt tgacccacat aaagagcaaa tcgctgttaa
     5401 agaagctaaa aagcttggta ttccagttgt agctatggtt gacactaacg ctgatccaga
     5461 tgatatcgat gttatcatcc cagcaaacga tgacgctatt cgcgccgtta aattaattac
     5521 ttctaaatta gctgatgcag ttattgaagg tcgtcaaggt gaagatgctg acgttgactt
     5581 cgctcaagaa gcacaagcag attcaattga agaaatcgtt gaagtagttg aaggatcaaa
     5641 aaacgactaa tatttaattt attgtaaatc ctaaaacggg gcagatgcaa accgctctgt
     5701 ctcgtttttt taatcattat ttgaaattaa gaggagaacg aaaatggcag aaatcacagc
     5761 taaacttgta aaagaattgc gtgaaaaatc aggtgcaggc gttatggacg ctaaaaaagc
     5821 attagtagaa actgatggtg accttgataa agcgattgaa ttacttcgcg aaaaaggtat
     5881 ggctaaagca gctaaaaaag cagaccgtgt tgctgctgaa ggtttaacag gtgtttatgt
     5941 tgatggtaac gttgcagcag ttattgaagt taacgctgag actgactttg ttgctaaaaa
     6001 cgaccaattt gtaacattag ttaatgaaac tgctaaagtt attgcagaag gacgtccttc
     6061 aaacaatgaa gaagcacttg ctttaacaat gccttcaggt gaaactttag aacaagcatt
     6121 tgtaactgca actgctacaa ttggtgaaaa aatttcattc cgtcgcttcg ctttagttga
     6181 aaagactgat gagcaacatt ttggtgcata ccaacataat ggtggacgta tcggagtaat
     6241 tactgttgtt gaaggtggcg acgatgcact tgctaaacaa gtatcaatgc acgttgcagc
     6301 tatgaagcca actgttcttt catatactga acttgatgca caatttgttc atgatgagtt
     6361 agctcagttg aaccacaaaa ttgagcaaga caacgagagt cgtgctatgg ttaacaaacc
     6421 agctcttcca ttccttaaat atggatcaaa agctcaattg actgatgaag ttattgccca
     6481 agcagaagaa gacatcaaag cagaattagc tgcagaaggt aaaccagaaa aaatctggga
     6541 taaaatcgtt ccaggtaaaa tggatcgttt catgcttgat aacactaaag ttgaccaaga
     6601 gtacacactc cttgctcaag tttacatcat ggatgatagc aaaacagttg aagcttatct
     6661 tgagtcagta aatgctaaag cagttgcttt cgtacgtttt gaagttggag aaggtattga
     6721 gaaagcttct aacgactttg aagcagaagt tgcagctaca atggcagcag cacttgaaaa
     6781 ataattaaca ctcgctttat attataattt ggcagcaaaa tgttgtcagt aaatatattt
     6841 taaaataaga cacttggatt aagtcaagtg tcttattttg gtatcgaatt ggtggattgg
     6901 caaatattaa aaaaggagag acagtcatct caccttaact tttctagaat aggttgaagc
     6961 cgataatgga taatgttccc atgatgcatc cagctagtat aacacctagc ataacagcaa
     7021 taacactatg tttaaattcc caatctagga ggctagctgc aagcgtgcca gtccaggctc
     7081 ctgttccggg taagggaatg cctacaaaca atagcaaggc aataaacaat cctttttctc
     7141 cagctacttt ttcaagtttt tgaccaccgc tatgtccttt tttaaggcac catgtgaaaa
     7201 acttaccagt ataaggttta tctgctcccc attctagaac tttgcgagca aagaagaaga
     7261 tgataggtac aggtagcatg tttccaacaa caccgatagc tagcgcttcc cacaaaggta
     7321 ttccattagc gattgcaaat ggcacagctc ctcgtagttc cactaaggga atcattgaaa
     7381 tcaaaaatgt aataatgtaa ttcattaaat aaaacctttc tatggtatta ttccatttta
     7441 tcttatcttg ctagtaatga caactaaaat ccgcggtcat tctagtttta ctatccttag
     7501 atagatttca tttccgtatc ttttaaaaat ttgatataat aatagtcagt tatagtcaaa
     7561 gaggaaggag tgaatttgtg gccataaaaa atacttccga taatattgaa gaatatataa
     7621 aatcattatt agaacaatca ggaattgctg aaataaagcg ttccaattta gctgatactt
     7681 ttcaagttgt tcctagtcag attaattatg tcattaagac acggtttact gaaagccgtg
     7741 gttatgtggt tgagagtaaa cgaggtggtg gaggttacat ccgcattgct aaggttcatt
     7801 tttctgatca acaccaatta tttggaaata tgttatcaac gattggcgag cgtatcagtg
     7861 aacaagtctt tgatgatttg attcaattat tattcgatga agagataatt acagaacgtg
     7921 aaggaaattt gattctggcg acatcaggtg atgacgtctt aggagagcaa gcctctgtta
     7981 ttagagctcg tatgctccgt aaattattac aacgattaga tagaaaagga ttccatttat
     8041 gagtcattat tccataaaat tacaagaagt attcagattg gcgcaatttc aagcagcacg
     8101 ctatgaaagt cactatttag aaagctggca tcttctttta gcaatggttt tagttcatga
     8161 ttcagtagct ggtttgacat ttgctgaata tgagtctgaa gttgcaattg aagagtacga
     8221 agcagcaaca attttagctt taggtagagc accgaaagaa gagattaccg attatcaatt
     8281 tttagagcaa tcatcggctt tgaaaaagat cttaaagtta gcagaaaata ttagtatcgt
     8341 agtaggagct gaagatgtgg gtactgaaca tgtcttgctt gctatgttgg ttaataaaga
     8401 tttattagcc acacgtatct tagaattagt tggttttaga ggtcaagacg atggtgaatc
     8461 agttcgtatg gttgatttac gtaaggctct agaacgtcat gccggtttta caaaagatga
     8521 tatcaaagca atttatgaat tacgtaatcc taaaaaagca aagtcgggtg cttctttttc
     8581 tgatatgatg aaaccaccga gtactgctgg tgatttagct gatttcacac gtgatttatc
     8641 acagatggcg gttgatgggg aaattgaacc tgttattggg cgtgatacag aaatttcacg
     8701 tatggtccaa gtcttaagtc gtaaaacaaa gaataatcct gtcttagttg gagatgcggg
     8761 tgtcggtaaa acagctttgg cgtacggtct agcacaacgt attgctaatg gaaatattcc
     8821 ttatgagctt agagatatgc gtgttctaga gttagatatg atgagcgttg ttgctggaac
     8881 tcgttttcgt ggtgattttg aggaacgtat gaatcaaatc attgcagata ttgaagaaga
     8941 cggtcatatt attcttttca ttgatgaact gcatactatt atgggttctg gctctggtat
     9001 tgatagcaca cttgatgctg ctaatatttt aaaaccagct ttggcccgag gcactttacg
     9061 cactgttgga gcaacaactc aagaggaata tcaaaagcat attgaaaaag atgctgcttt
     9121 atctcgtcgc tttgcaaaag ttttagtaga agaaccaaat cttgaagacg cttatgaaat
     9181 tctacttgga ttgaagccag cgtatgaagc ctttcataat gttactattt cagatgaagc
     9241 agttatgact gctgtaaaag tggctcatcg ttatttgaca agtaagaatt taccagactc
     9301 agctatagac ttacttgatg aagcttctgc cactgtgcaa atgatgatta agaaaaatgc
     9361 tccttcgctt ttaacagaag ttgaccaagc tattttagat gatgatatga aatctgcttc
     9421 taaagctcta aaagcaagcc ataaggataa aaagcgtaag cctattgccg taaccgaaga
     9481 tcatattatg gcaactctca gccgtctatc tggtattcct gttgaaaaat tgacacaagc
     9541 tgatagtaaa aaatacctca atttagaaaa agaattgcat aaacgtgtta tcggtcaaga
     9601 tgatgcagtt accgccatta gtcgtgctat ccgtcgcaat caatctggca ttcgtactgg
     9661 taagcgtcct attggttcct ttatgttctt gggaccaact ggtgttggta agacagagtt
     9721 agcaaaagcc ttggcagaag tgttgtttga tgatgaatca gccctaattc gttttgatat
     9781 gtcggaatat atggaaaaat tcgcagcttc tcgcctgaat ggggcacctc caggttatgt
     9841 tggttatgat gaaggaggag agctgactga aaaagtacgc aataaaccat attcagtttt
     9901 actttttgat gaagttgaaa aagctcatcc agatattttt aatgtattat tacaagtgct
     9961 tgatgatggc gttttaacag atagtcgagg acgaaaagtt gatttttcaa atactataat
    10021 cattatgact tctaatttag gagcaactgc attacgtgac gataaaacag ttggttttgg
    10081 agctaaggat atcagccatg attatactgc tatgcaaaaa cgtattatgg aggagcttaa
    10141 aaaagcttat cgaccagaat ttatcaatcg tattgatgag aaagttgtat tccatagtct
    10201 cagtcaagat aatatgcgtg aagttgtgaa aattatggtt aaacctttaa ttttagcgct
    10261 taaagataaa ggtatggatc tgaaattcca gccttcagcc ttgaaacact tggcagagga
    10321 tggctatgat atagaaatgg gtgctcgtcc actgcgtaga actattcaaa cgcaagtaga
    10381 ggatcattta tcagaactac tattggcaaa tcaagtgaaa gaggggcaag taattaagat
    10441 tggcgtatcc aaaggcaaat tgaaatttga tattgcaaaa tcataaaaca ttccagtacc
    10501 gatgggtact ggaattttaa tata
//
LOCUS       DATRNK010000064         9578 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.064, whole genome shotgun sequence.
ACCESSION   DATRNK010000064 DATRNK010000000
VERSION     DATRNK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9578
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.064"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(179..2458)
                     /gene="pcrA"
                     /locus_tag="VBC52_001681"
     CDS             complement(179..2458)
                     /gene="pcrA"
                     /locus_tag="VBC52_001681"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEO6883248.1"
                     /translation="MNPLIIGMNDKQSKAVQTTDGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKYVNPWNILAITFTNKAAREMRERAIALNPATQDTLIATFHSMCVRILRREADYI
                     GYNRNFTIVDPGEQRTLMKRIIKQLNLDTKKWNERSILGTISNAKNDLLNEIAYEKQA
                     GDMYTQVIAKCYKAYQEELRRSEAMDFDDLIMMTLRLFDQNKDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPQAKVVL
                     LEENYRSTKKILQAANNVINHNKNRRPKKLWTQNDEGEQIVYHRANNEQEEAVFVAST
                     IDNIVREQGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVIA
                     YLNILANTSDNISFERIVNEPKRGVGPGTLEKIRSFAYEQSMSLLDASSNVMMSPLKG
                     KAAQAVWDLANLILTLRSKLDSLTVTEITENLLDKTGYLEALQVQNTLESQARIENIE
                     EFLSVTKNFDDNPEITVEGETGLDRLSRFLNDLALIADTDDSATETAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRAIEDADELEEERRLAYVGITRAEQILFLTNANTRTLF
                     GKTSYNRPTRFIREIDDELIQYQGLARPVNSSFGVKYSKEQPTQFGQGMSLQQALQAR
                     KSNSQPQVTAQLQALNTNNSHETSWEIGDVATHKKWGDGTVLEVSGSGKTQELKINFP
                     GIGLKKLLASVAPISKKKN"
     gene            complement(2564..2950)
                     /locus_tag="VBC52_001682"
     CDS             complement(2564..2950)
                     /locus_tag="VBC52_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEO6883249.1"
                     /translation="MKFNLEQVKVFENYEIENWEEGQVTLTTKVVDSSLNYYGNAHGG
                     YLFTLCDQVSGLVAISTGYEAVTLQSNINYLRAGRLDDLLTVIGTCVHNGRTTKVVDV
                     HIKNQKGEMVTTAIFTMFVTGERKEK"
     gene            complement(3083..4408)
                     /locus_tag="VBC52_001683"
     CDS             complement(3083..4408)
                     /locus_tag="VBC52_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEO6883250.1"
                     /translation="MTLNAKGESNQILVDKEVHSTNLLLDIDEKPELFQGLLLSFQHV
                     FAMFGATILVPLILGMPVSVALFASGCGTLIYQVATKFKVPVYLGSSFAYITAMALAM
                     KQMHGDISAAQTGILFVGLIYVVVATVIKFVGNSWVDKILPPIIIGPMIIVIGLGLAN
                     SAVTNAGFVAKGDWRKMLVAVVTFLIAAFINTKGKGFIKIIPFLFAIIGGYILSIILG
                     LVDLSPVEKAAWFELPKFYLPFKTGLFHSYKLYFGPEMLAILPISIVTIAENIGDHTV
                     LGQICGRNFLKKPGLNRLLIGDGLATAFSALIGGPAETTYGENTGVIGMTRIASVTVI
                     RNAAFIAIAFSFFGKFTALISTIPSAVLGGMAILLYGVIASNGLKVLIENRVNFAEVR
                     NLIIASSMLVLGLGGAVLDLGALTLSGTALSAIVGIILNLILPKESMKS"
     regulatory      4716..4848
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            5005..6351
                     /locus_tag="VBC52_001684"
     CDS             5005..6351
                     /locus_tag="VBC52_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEO6883251.1"
                     /translation="MLTLFTHINSFVWGPPLLALLVGTGIYLSFRLGFIQLRQLSRAF
                     KLIFREDNGQGDISSYAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGLLAIKYRTKDTNGEISGGPMYYIVNGMGQKWKPLAVFFSAAGILVALLGIG
                     TFTQVNAITSSLEHTFKISTRFTSLILAVIVLFIIFGGIKSISKVSEKIVPFMAISYI
                     LATLIIIAVNYNKIPHTFQLIFSGAFSGTAAIGGFSGAIVKEAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTKEPVEQGLISMTGTFIDTIVICTLTGIAILVTGKWLEFDLQGAPL
                     TQASFNTVFGSLGSFALTFCLVLFAFTTILGWSYYGERCFEYLFGTKFINAYRIIFVI
                     MVGLGGFLQLDLIWVIADIVNGLMALPNLIALLVLSPIIVKETQKYFSETKNITKP"
     gene            6415..7650
                     /locus_tag="VBC52_001685"
     CDS             6415..7650
                     /locus_tag="VBC52_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO6883252.1"
                     /translation="MATSTENLQLAKRGPIISIIAYITLAVAKLAAGYWFDATSLVAD
                     GFNNLSDILGNVALLIGLHLASQPADSNHRFGHWKIEDLASLITSFIMFVVGIQVFIQ
                     TVTKIINNTDTNIDPLGAIVGAISALVMLGVYLYNKQLSQRVKSSALVAASKDNLSDA
                     VTSIGTSIAIIAASLNFPIIDRLAAIIITYFILKTAYDIFIESAFSLSDGFDDYQLKQ
                     YEKAILTIPKISAVKSQRGRTYGSNIYLDIVLEMNPDLSVFESHAITERVEKLLSDKF
                     SVYDIDIHVEPASIPEDEIFDNVYQKLYKNEKIILAKIPGYETFISPDFYMINEKGNI
                     ITSDMLTNATNHSLASNFKYFNVKSISQKTKLVSYELEGKRHTSIWRRNEKWFLIYHQ
                     ITAKSSPYKTRRYQITSLD"
     gene            7809..8201
                     /locus_tag="VBC52_001686"
     CDS             7809..8201
                     /locus_tag="VBC52_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO6883253.1"
                     /translation="MKLYVQLMVILTFSFAGEVLSTIFNLPVPGSIIGLILLFLALKY
                     KIIRLRHIDAVGNFLLANMTILFLPPAVGLMEHFQDIKPYLFGIAIIILGALFLNILT
                     IGLVSQWIKKRYEGDYPEIGGKNGNFNE"
     gene            8182..8877
                     /locus_tag="VBC52_001687"
     CDS             8182..8877
                     /locus_tag="VBC52_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEO6883254.1"
                     /translation="MATLTNNPIFGIMLTVWAYYIGIRIFRKYPSPATTPLLLATILL
                     IAFLKLTHISYKDYYNGGSFLTMLITPSTVVLAIPLYRTFHLMKHHIKSISISIILAS
                     VINTVFTAIVAKFFGMKYFLAISLFPKSVTTAMAVGITSKAGGLATITLVVVVITGIL
                     TSVLGPIFLKLLRIEDPVAIGLALGGTGHAIGTGQALKYGQVQGAMAGLAIGITGICY
                     VIVSPLVAGLILK"
     gene            8946..9569
                     /locus_tag="VBC52_001688"
     CDS             8946..9569
                     /locus_tag="VBC52_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEO6883255.1"
                     /translation="MIPLKKITVGTIVCLSFLGLTACSSSNTQQTSTSKSNVSQHKNI
                     KADHEELRLKFNKVKLGVKANNFKGGTSLAELKQLFGGEPNEKFDTPAGNVTLKGYRW
                     NVDDISITIQLLNDSSIVRSISNFKFIRDANITTKDYNSLKNGMSYNKVKELLGEPDD
                     ISQAVSSDKEELQAAWISGIQSSDSDPGINLTFENDKLTNKQQHGLK"
BASE COUNT         3092 a         1766 c         1570 g         3150 t
ORIGIN      
        1 tgcaagagaa acgcttcttt ttttaaaata gtatggtcac ttttagaaaa cgctttaata
       61 tcaacctttt tagtaagtaa aaaccaaatg ataacttatt ttttctattc aaatgatgca
      121 aaaagactaa gaatacaatt cttagtctct gattatttaa gcatataata gaagaaagtt
      181 agtttttctt tttgctaata ggagcgacac tcgctaataa tttctttaac ccaatacctg
      241 gaaaatttat ttttaactct tgagtctttc cgctacctga aacttcaagc accgtaccgt
      301 ccccccattt tttatgagtt gcaacatcac caatctccca tgatgtctca tggctattgt
      361 tcgtgttaag agcctggagt tgggcagtta cttgaggttg tgaattactt ttacgtgctt
      421 gaagcgcttg ttgaagactc attccttgac caaattgtgt aggctgttct tttgaatatt
      481 tcacaccaaa tgaagaatta actggtcgag ctaacccttg atattgtatc agctcatcat
      541 caatctcacg tataaaacgg gttggtctat tataactagt tttaccaaat aaagtacggg
      601 tattagcatt ggtaaggaaa agtatttgtt ctgctctcgt aatcccgaca taagctaaac
      661 gacgctcttc ttctaattca tcagcatcct ctattgcacg cgataaagga aatactcctt
      721 cttccatacc gattaagaaa acaactggaa attctagacc cttggcagca tgtaatgtca
      781 ttaaagtgac ctcagccgtt tcagtggcgc tatcatcagt atctgctatt aaagctaaat
      841 catttaaaaa acgcgataga cgatctaaac cagtttcacc ctctactgta atctcagggt
      901 tatcgtcaaa atttttagtc actgacaaga attcttcaat attttcaata cgcgcttgac
      961 tttctaatgt attttgaact tgaagtgctt ctagatatcc tgtcttatcc agtaggtttt
     1021 ctgtaatctc cgtcacagtt aaactatcta gcttactacg tagagtcaaa atcagattag
     1081 ctaaatccca aacagcttga gcagccttcc cttttaaggg cgacatcatg acatttgaag
     1141 aggcatcaag aagagacatg ctctgttcat aggcaaatga ccgtattttt tctaaagttc
     1201 ctggccccac ccctctttta ggttcattta caatccgctc aaaagaaata ttatcagaag
     1261 tatttgcaag gatattgaga taagcaataa catctcgaat ttctttgcga ctatagaatt
     1321 tcgttccacc aaccattgta tatggaatat ttgattttaa aagtgcttcc tcaatagtac
     1381 gagattgcgc attcgtacga taaagaacgg caaaatcttt gaaatttttt ccttgttctc
     1441 gaacgatgtt atcaatagtt gatgctacaa aaacggcttc ttcttgctca ttgttagctc
     1501 tatgatatac aatttgctca ccttcatcat tttgagtcca taatttttta ggacggcgat
     1561 ttttattatg attgatcaca ttattagcag cttgaagtat tttcttagtt gagcgataat
     1621 tttcttctaa taatacaact tttgcttgcg gatagtcctt ttcaaaatca agaatatttt
     1681 gcatatcagc tccacgccat ccataaatag attgatcggc atcaccaaca acacaaatat
     1741 ttttgaaacg cgaagctaat aacttaacta attggtattg agcgtggtta gtatcttgat
     1801 actcgtctac atggatatat tgatacctct gttggtaata ggccaaaaca tctttattct
     1861 gatcaaatag tcgaagtgtc atcataatca agtcatcgaa atccatggcc tcacttctac
     1921 gtaattcctc ttgataagct ttatagcatt tagcaattac ctgtgtatac atatctccgg
     1981 cttgcttctc atatgcaatt tcattaagaa ggtcattttt agcattagag attgtaccta
     2041 aaattgaacg ttcattccac ttttttgtgt ccaaatttaa ttgcttaatg attcgcttca
     2101 ttaaagtacg ctgctcacct ggatcaacga ttgtaaagtt acgattatat ccaatataat
     2161 cagcttcacg acgtaaaata cgaacacaca tactatgaaa agttgcaatc aaagtatctt
     2221 gggtagctgg attcaaggcg atagcacgtt cacgcatttc acgcgctgct ttattagtaa
     2281 aagtaatcgc taaaatattc caaggattaa catatttttc atctattaaa taagcaatac
     2341 gatgagtcag gacacgtgtt tttccagagc cagctcctgc cataatcaaa agcggtccgt
     2401 ctgtcgtttg taccgctttt gattgtttat cattcattcc aattattaaa ggattcattt
     2461 ctactcctaa ttttgtcaaa attgcttctc tagtatatca ttttttcatc tttttcccaa
     2521 tagaaaaagg agttaaacag acgctgtcta actcctacct tttttacttc tcttttcttt
     2581 ctccagttac aaacattgta aaaatagcag ttgtaaccat ttcgcctttc tgatttttaa
     2641 tgtgaacatc aacgactttt gtagtacgac cattatgaac acaagtaccg atgacagtaa
     2701 gcagatcatc taatcggcct gccctcaaat agttgatatt tgattgtaga gttaccgctt
     2761 catatcctgt tgaaatagca actaaaccac tgacttgatc acacagtgta aacaaataac
     2821 caccatgagc attaccataa tagttgagcg aagagtcaac gaccttcgtt gttaaggtaa
     2881 cttgaccttc ttcccagttc tcaatttcgt aattctcaaa tacttttact tgttctaaat
     2941 tgaatttcat tctactataa cctcactttt tctatgacca ttatagcaga aaataaggaa
     3001 accataactg taaacgtttt tattttaaac tttttaactg acctatcata acaaaagagc
     3061 tcagatactc tgagctcttt tgttatgatt tcatgctttc ttttggcaat attaaattta
     3121 atataatacc cactatagca cttaatgctg ttcctgataa agttagagca cctagatcaa
     3181 ggactgctcc accgaggcct aaaactaaca tggaactggc aataattaga ttacgaacct
     3241 cagcaaaatt gacacgattt tcaatcaaca cttttaatcc attacttgca ataacaccat
     3301 aaagtaatat agccattcca ccaagtactg cactaggaat tgtagagatc aaagctgtaa
     3361 atttaccaaa gaatgaaaaa gcgattgcaa taaaagctgc attacgaata accgttacag
     3421 aagctatacg cgtcatacca ataacacctg tattttcacc ataagtagtt tcagctggtc
     3481 ctcctataag agcagaaaat gcggttgcta aaccatctcc aattaaaaga cgatttaaac
     3541 ctggtttttt tagaaagtta cgcccacaaa tctgacctaa aactgtatga tcaccgatat
     3601 tttcagcaat cgttacaatt gagattggaa ggattgcaag catttctggt ccaaagtaaa
     3661 gtttataact gtgaaataaa cctgtcttaa acggtaaata gaattttggt aattcaaacc
     3721 aggcagcttt ttcaactgga gataaatcta ccaagccaag aatgattgat aaaatataac
     3781 ctccaataat tgcaaataaa aatggaataa tcttgataaa acctttccct ttagtattga
     3841 taaaagctgc aatcaagaat gtaacaacag caactagcat tttacgccaa tcacctttag
     3901 cgacaaaacc tgcattagtt acagcagagt ttgccaaacc taaaccaata acaataatca
     3961 taggtccaat gattattggt ggcaaaatct tatcaaccca tgaatttcct acgaacttaa
     4021 taaccgtcgc cacaacgaca tagattagac ctacaaaaag aatacctgtt tgagcagccg
     4081 aaatatcgcc atgcatttgt ttcatggcta atgccatagc tgtaatataa gcaaatgatg
     4141 agcctaggta aacagggact ttaaattttg tcgcaacttg ataaatgagc gttccgcaac
     4201 cagaagcaaa taaagccact gatacaggca tccctaaaat aagcggaact aatatcgtag
     4261 ctccaaacat ggcaaaaaca tgttgaaaac ttaacaacaa accttgaaat aattctggtt
     4321 tttcatcgat atctagtaat aagttagtag aatgtacttc cttatctact agaatttgat
     4381 tactttcacc tttagcattt aaagtcatat ctaaactagt cctcttgggc atacaaaaaa
     4441 gcccactttc tatagcaata tagtcatagg ctttattttt ttcctgatgt ttttctcacc
     4501 tcacgggatg agtttaaaaa cctacgctga aagaattata gcataagaca ttttagaaaa
     4561 caagactttt ttgaaactat attgacaaaa ctatgctttt tcattatgat agttaacata
     4621 ataaaatata ccgaatgacg tcattcagga gaagaaagtt agactttcgc cgaaggaatt
     4681 acactctcag gtgtcttaag acaggactga ttgacagacg gacttctgga gagacctata
     4741 agtagcaaca tctttatatt gacatcaaga tgtgctctag gcgccgaagg ggcaagaaga
     4801 gtaaaacaac tcctccaatc tctcaggcaa aaggacagaa gctaaaagcc aatattaata
     4861 atgagtagta agcttattaa gtttactact acctttattt gtgcgctttt tagctagcat
     4921 ctttcagaag ttatctcttt tagagataac ttttttcgtt tcattacaga atccataggt
     4981 atgtcatgta tcaaaggaga acatatgcta acacttttta ctcatatcaa tagcttcgtt
     5041 tggggtccac ctttacttgc tttattagtc ggaacaggta tttacctatc atttcgctta
     5101 ggttttattc aattgagaca actttctaga gctttcaaat tgattttccg agaggataac
     5161 gggcaaggag atatttcaag ttatgctgcc cttgcaactg ctcttgctgc aacggtaggg
     5221 acaggtaata tcgttggtgt ggctacggct attaaatctg gaggaccagg agctttgttt
     5281 tggatgtggg tagccgcctt ttttggaatg gcaacaaaat acgcagaagg attattagct
     5341 atcaaatatc gcacaaaaga tactaatgga gaaatttctg ggggaccaat gtactacatt
     5401 gtcaatggaa tgggacaaaa atggaaacca ttagcagtct ttttttctgc agcaggtatc
     5461 ttagtagctc ttttaggaat agggacattc actcaagtca atgcaattac ctcttctctt
     5521 gagcatactt tcaaaatatc aactagattt accagcctca ttcttgctgt tatagtatta
     5581 tttattatat ttggaggtat aaaatctatt tcaaaagttt cagaaaaaat agtgcctttt
     5641 atggctataa gctatatttt agcaactctg attattattg cagttaatta caataagatt
     5701 ccacatactt tccaactcat ctttagcggt gctttttctg ggacagcagc cattggtggt
     5761 ttttctggag ctatcgttaa agaagcaatt caaaagggaa ttgctagagg agttttctca
     5821 aatgaatctg gattgggttc tgctccaata gctgcagcag cagctaaaac aaaagaacct
     5881 gttgaacaag ggcttatttc aatgacagga accttcattg acactattgt tatttgtact
     5941 ttgacaggga ttgcaatttt agttacagga aaatggctag aatttgattt acagggtgct
     6001 ccattaacac aagcatcttt taataccgta tttggctcac taggtagttt tgctctaacc
     6061 ttctgccttg tcttatttgc ttttactact attttaggat ggagctacta cggagagcgt
     6121 tgctttgaat atctatttgg gactaaattt attaacgctt atcgtattat ttttgtcatt
     6181 atggttggct taggtggttt cttacagctc gatcttattt gggttatcgc tgatatcgtt
     6241 aatggtttaa tggctttacc aaatctaata gctctcctag ttttgtcacc aattattgtt
     6301 aaagaaacac agaaatattt ttcagagact aaaaatatca ctaagccttg aacttactag
     6361 ttttatagtc tgctcctaag tgatataatg tgtagatgaa aggaaattag gaaaatggca
     6421 acttctactg aaaacttaca acttgcaaaa agagggccta ttattagtat cattgcctat
     6481 ataacactgg cagtcgctaa attagctgca ggttattggt ttgatgctac ttcacttgta
     6541 gcagatggat ttaataactt atctgatatc ttaggaaatg tagcactctt aattggttta
     6601 cacttggcta gtcaaccagc cgactcaaac catcgttttg gtcactggaa aattgaagat
     6661 ttagcaagcc tgatcacctc ttttatcatg tttgttgtag gtattcaagt ttttattcaa
     6721 actgtcacta agattattaa taatactgat actaacattg atcctctagg tgctatagta
     6781 ggtgctatat ctgctcttgt tatgcttggc gtttatctct ataataaaca attatctcaa
     6841 agagtaaagt ctagcgctct ggttgcagct tctaaagata acctttctga tgctgtgaca
     6901 tctatcggca catctattgc tatcattgca gcttctctaa attttcctat tattgatcgt
     6961 ttagctgcta tcattattac ttattttatt cttaaaacag cttatgatat ttttatagaa
     7021 tctgctttca gcttatcgga tggtttcgat gattatcaat taaaacaata cgaaaaagct
     7081 atattaacaa ttcctaaaat atccgctgta aaatctcaac gtggacgtac ttacggtagt
     7141 aatatttacc ttgatattgt tctcgaaatg aaccctgatc tctcagtctt tgaaagtcat
     7201 gccattaccg aaagagtgga aaaactactc agtgataaat tttctgtata tgacatagat
     7261 attcatgtag aacccgcttc tattccagaa gacgaaattt tcgataatgt ttaccaaaaa
     7321 ttatacaaaa atgaaaaaat catcctagca aaaattcccg gttatgagac ctttattagc
     7381 cctgactttt atatgattaa tgaaaaggga aatattatca cttctgacat gcttactaat
     7441 gctacaaatc attcattggc aagcaacttt aaatatttca atgtaaaatc aattagtcaa
     7501 aaaacaaaac ttgtgtctta tgaattggaa ggtaaacgac atactagtat ttggcgccgt
     7561 aatgaaaaat ggttcttaat ctaccatcaa ataactgcca agtcaagtcc gtataaaacg
     7621 agacgatatc aaatcacttc ccttgattaa tatactattt aatcagttac tcttgataaa
     7681 aatagcttac aaaaataaat taaaaagatt gagaaggatc tcggtctttt taatttgttt
     7741 ttatgtaaac gtttcatagc cttgaaaatt actttcagag tagtagacta taatggaagg
     7801 agtttttcat gaaattatac gttcaattaa tggttatttt aaccttttca ttcgctggtg
     7861 aggttctatc tactatattc aacttacctg taccaggtag tattattgga ttaatattac
     7921 tattcctagc gttaaaatac aaaatcatca ggctcaggca tattgatgcc gtggggaatt
     7981 ttctattagc aaatatgacc attttatttt taccaccagc agttggactg atggagcact
     8041 ttcaagacat aaagccctat ctttttggaa tagctatcat tatcttagga gcgctatttc
     8101 tcaatattct aactattgga ctggtatccc aatggatcaa aaagagatat gaaggagatt
     8161 atccagaaat tggaggtaaa aatggcaact ttaacgaata accctatctt tggtattatg
     8221 ttaaccgtat gggcgtatta cattggcata aggattttta gaaaatatcc ttcccctgca
     8281 acaactccac ttcttttggc gactatttta ttaattgctt ttttaaaatt aacacatatc
     8341 tcctataaag attactacaa tggcggttca tttctaacga tgttaattac gccttcaacg
     8401 gttgtattgg ctattcctct ctatagaacc tttcatttaa tgaagcatca cattaaaagt
     8461 attagcatta gcatcatctt agcttctgtt atcaacacag tttttaccgc aatagttgct
     8521 aagttttttg gtatgaaata tttcttagca atctcacttt tcccaaagtc cgtaactaca
     8581 gctatggccg ttggaattac aagtaaagca ggtggattag ctactatcac tctggtcgta
     8641 gttgttatta caggcatctt aacaagcgtt ttgggtccta tttttctaaa attattaagg
     8701 atagaagatc ctgttgctat agggctcgcg ctaggaggaa ctgggcatgc tataggaact
     8761 ggacaagcct taaaatatgg ccaagttcaa ggcgccatgg ctggactagc aattggtatc
     8821 acagggattt gctatgttat tgtcagtcct ttagttgcag gcttaatatt gaaatgagga
     8881 taaggaaagt aaacccaaaa taattatgtt ataatattta aaaatagaat ttaggataaa
     8941 taattatgat accgttaaaa aaaataactg ttggaacgat tgtctgtctc tcatttttgg
     9001 gtttaacagc atgctcttct tcaaatactc agcaaacctc tacttcaaaa tcaaatgtat
     9061 cacaacataa aaatatcaaa gctgaccacg aagaattacg tttgaaattt aataaagtaa
     9121 agttaggggt taaagcaaat aactttaaag gaggcactag tctcgctgaa ttaaaacaat
     9181 tatttggtgg cgaaccaaat gaaaaatttg atacaccagc gggaaatgtc actcttaaag
     9241 gctatcgttg gaatgtcgat gatatctcca tcactataca attattaaac gatagtagca
     9301 ttgttcgttc aattagtaat tttaagttta ttcgtgatgc caacatcaca acaaaagatt
     9361 ataatagttt aaaaaatggc atgtcataca ataaagtaaa agaacttcta ggagaacctg
     9421 atgatatttc tcaagcagtt tcttcagata aagaggaact acaggctgca tggattagtg
     9481 gcatccagtc gtcagacagt gatccgggaa ttaaccttac atttgaaaat gataaactca
     9541 ctaacaaaca acagcatggt ttaaaataaa aaggcatt
//
LOCUS       DATRNK010000065         9553 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.065, whole genome shotgun sequence.
ACCESSION   DATRNK010000065 DATRNK010000000
VERSION     DATRNK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9553
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.065"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            310..3634
                     /locus_tag="VBC52_001689"
                     /pseudo
     CDS             310..3634
                     /locus_tag="VBC52_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            3870..4790
                     /gene="lmb"
                     /locus_tag="VBC52_001690"
     CDS             3870..4790
                     /gene="lmb"
                     /locus_tag="VBC52_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEO6883256.1"
                     /translation="MKKGFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVDVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGRLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
     gene            4803..7271
                     /locus_tag="VBC52_001691"
     CDS             4803..7271
                     /locus_tag="VBC52_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO6883257.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGLATKDNQIAYIDD
                     SKGKAKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYRFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPGRRKAPIPDVTPNP
                     GQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTEDNDSGSEHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELAAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YMMPKDGKDYFYARDQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRDQIATVKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFATPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNTGDATDTDKPKEKQQAD
                     KSNENQQPSEASKEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIFQPE
                     GVQFYNKNGELVTYDIKTLQQINP"
     gene            complement(7457..7696)
                     /locus_tag="VBC52_001692"
                     /pseudo
     CDS             complement(7457..7696)
                     /locus_tag="VBC52_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7736..8868)
                     /locus_tag="VBC52_001693"
                     /pseudo
     CDS             complement(7736..8868)
                     /locus_tag="VBC52_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(9092..>9553)
                     /locus_tag="VBC52_001694"
     CDS             complement(9092..>9553)
                     /locus_tag="VBC52_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSag2 family transposase"
                     /protein_id="HEO6883258.1"
                     /translation="ALVTDLTYVRAGNRWAYVCLIIDLYNREIIGLSLGWHKTAELVK
                     QAIQSIPYALTKVKMFHSDRGKEFDNQLIDEILEAFGITRSLSQAGCPYDNAVAESTY
                     RAFKIEFVYQETFQLLEELALKTKDYVHWWNYHRIHGSLNYQTPMTKRLIA"
BASE COUNT         3171 a         1849 c         1911 g         2622 t
ORIGIN      
        1 tgatagaata atgagattaa ggaggtcaca aaataagcaa ctcttaaaaa gctgaccttt
       61 actaataata atctttgttt tataataaat atgttagtaa tataattaat aaatgagata
      121 catttaatca ttatgacaaa agcaagaaaa atagctgtat catatgcaaa taacctcctg
      181 tttgctcttt aaaaaagacg ttatccttat ttctccacgc acagatggat aactaggaga
      241 gaatcgtttg attctctctt ttcttaatgg tcataaagac aaagtctctc atcatgaaag
      301 gacgacacat tgcgtaaaaa acagaaacta ccatttgata aacttgccat tgcgcttatg
      361 tctacgagca tcttgctcaa tgcacaatca gacatcaaag caaatactgt gacagaagac
      421 actcctgcta ccgaacaaac cgtagaaact ccacaaccaa cagcggtttc tgaggaagca
      481 ccatcatcaa aggaaactaa aaccccacaa actcctagtg atgcaggaga aacagtagca
      541 gatgacgcta atgatctagc ccctcaagct cctgctaaaa ctgctgatac accagcaacc
      601 tcaaaagcga ctattaggga tttgaacgac ccttctcagg tcaaaaccct gcaggaaaaa
      661 gcaggcaagg gagctgggac tgttgttgca gtgattgatg ctggttttga taaaaatcat
      721 gaagcgtggc gcttaacaga caaagccaaa gcacgttacc aatcaaaaga agatcttgaa
      781 aaagctaaaa aagagcacgg tattacctat ggcgagtggg tcaatgataa ggttgcttac
      841 taccatgact atagtaaaga tggtaaaacc gctgtcgatc aagagcacgg cacacacgtg
      901 tcaggaatct tgtcaggaaa tgctccatct gaaacgaaag aaccttaccg cctagaaggt
      961 gcgatgcctg aggctcaatt gcttttgatg cgtgtcgaaa ttgtaaatgg actagcagac
     1021 tatgctcgta actacgctca agctatcaga gatgctatca acttgggagc taaggtgatt
     1081 aatatgagct ttggtaatgc tgcactagct tacgccaacc ttccagacga aaccaaaaaa
     1141 gcctttgact atgccaaatc aaaaggtgtt agcattgtga cctcagctgg taatgatagt
     1201 agctttgggg gcaagacccg tctacctcta gcagatcatc ctgattatgg ggtggttggg
     1261 acacctgcag cggcagactc aacattgaca gttgcttctt acagcccaga taaacagctc
     1321 actgaaactg ctacggtcaa aacagccgat cagcaagata aagaaatgcc tgttctttga
     1381 taagattgca aaagctaaaa aagctggtgc tgtaggagtc ttgatctatg acaatcagga
     1441 caagggcttc ccgattgaat tgccaaatgt tgatcagatg cctgcggcct ttatcagtcg
     1501 aaaagatggt ctcttattaa aagacaatcc ccaaaaaacc atcaccttca atgcgacacc
     1561 taaggtattg ccaacagcaa gtggcaccaa actaagccgc ttctcaagct ggggtctgac
     1621 agctgacggt aatattaagc cagatattgc agcacccggc caagatattt tgtcatcagt
     1681 ggctaacaac aagtatgcca aactttctgg aactagtatg tctgcgccat tagtagcggg
     1741 tatcatggga ctgttgcaaa agcagtatga gacacagtat cctgatatga caccatcaga
     1801 gcgtcttgat ttagctaaaa aagtattgat gagctcagca actgccttat atgatgaaga
     1861 tgaaaaagct tatttttctc ctcgccaaca aggagcagga gcagtcgatg ctaaaaaagc
     1921 ttcagcagca acgatgtatg tgacagataa ggataatacc tcaagcaagg ttcacctgaa
     1981 caatgtttct gataaatttg aagtaacagt aacagttcac aacaaatctg ataaacctca
     2041 agagttgtat taccaagcaa ctgttcaaac agataaagta gatggaaaac actttgcctt
     2101 ggctcctaaa gcattgtatg agacatcatg gcaaaaaatc acaattccag ccaatagcag
     2161 caaacaagtc accgttccaa tcgatgctag tcgatttagc aaggacttgc ttacccaaat
     2221 gaaaaatggc tatttcttag aaggttttgt tcgtttcaaa caagatccta aaaaagaaga
     2281 gcttatgagc attccatata ttggtttccg aggtgatttt ggcaatctgt cagccttaga
     2341 aaaaccaatc tatgatagca aagacggtag cagctactat catgaagcaa atagtgatgc
     2401 caaagaccaa ttagatggtg atggattaca gttttacgct ctgaaaaata actttacagc
     2461 acttaccaca gagtctaacc cgtggacgat tattaaagct gtcaaagaag gggttgaaaa
     2521 catagaggat atcgaatctt cagagatcac agaaaccatt tttgcaggta cttttgcaaa
     2581 acaagacgat gatagccact actatatcca ccgtcacgct aatggcaagc catatgctgc
     2641 gatctctcca aatggggacg gtaacagaga ttatgtccaa ttccaaggta ctttcttgcg
     2701 taatgctaaa aaccttgtgg ctgaagtctt ggacaaagaa ggaaatgttg tttggacaag
     2761 tgaggtaacc gagcaagttg ttaaaaacta caacaatgac ttggcaagca cacttggttc
     2821 aacccgtttt gaaaaaacgc gttgggacgg taaagataaa gacggcaaag ttgttgctaa
     2881 cggaacctac acctatcgtg tccgctacac tccgattagc tcaggtgcaa aagaacaaca
     2941 cactgatttt gatgtgattg cagacaatac gacacctgaa gtcgcaacat cggcaacatt
     3001 ctcaacagaa gatcgtcgtt tgacacttgc atctaaacca aaaaccagcc aaccggttta
     3061 ccgtgagcgt attgcttata cttacatgga tgaggatctg ccaacaacag agtatatttc
     3121 tccaaatgaa gatggtacct ttactcttcc tgaagaggct gaaacaatgg aaggcgctac
     3181 tgttccattg aaaatgtcag actttactta tgttgttgaa gatatggctg gtaacatcac
     3241 ttatacacca gtggctaagc tattggaggg ccactctaat aagccagaac aagacggttc
     3301 agatcaagca ccagacaaaa aaccagaagc taaaccagaa caagacggtt caggtcaaac
     3361 accagataaa aaaacagaaa ctaaaccaga aaaagatagt tcaggtcaaa caccaggtaa
     3421 aactcctcaa aaaggtcaac cttctcgtac tctagagaaa cgatcttcta agcgtgcttt
     3481 agctacaaaa gcatcaacaa gagatcagtt accaacgact aatgacaagg atacaaatcg
     3541 tttacatctc cttaagttag ttatgaccac tttcttcttg ggattagtag ctcatatatt
     3601 taaaacaaaa cgccaaaaag aaactaaaaa atagtattac ttaaactgat aagagcttat
     3661 ttaatagaga gaaccaattt ggttctctct atttttttat atgtgctacc tcatcaaaaa
     3721 gaagttagga caaatagctg tcaacaattg ccttttgctt atcccttcag ttgatgtgat
     3781 aaaagatggt agttgccatt gacaaagcat tgataaagga gtaaaattaa ctggttaata
     3841 actggttaaa ttataattga ggaggtacta tgaaaaaagg tttttttctc atggctatgg
     3901 ttgtgagttt agtaatgata gcagggtgtg ataagtcagc aaaccccaaa cagcctacgc
     3961 aaggcatgtc agttgtaacc agcttttacc caatgtatgc gatgacaaaa gaagtatctg
     4021 gagacctcaa tgatgtgagg atgatccaat caggtgcagg cattcattcc tttgaaccgt
     4081 ctgtaaatga tgtggcagct atttatgacg cggatttgtt tgtttaccat tcacatacct
     4141 tagaagcttg ggcaagggat ctagacccta atttaaaaaa atcaaaggtt gatgtgtttg
     4201 aagcgtcaaa acctttgaca ctagatagag tcaaagggct agaagatatg gaagtcacac
     4261 aaggcattga ccctgcgaca ctttatgacc cacatacctg gacggatccc gttttagctg
     4321 gtgaggaagc tgttaatatc gctaaagagc taggacgttt ggatcctaaa cacaaagaca
     4381 gttacactaa aaaggctaag gctttcaaaa aagaagcaga gcaactaact gaagaataca
     4441 ctcaaaaatt taaaaaggtg cgctcaaaaa cattcgtgac gcaacacacg gcattttctt
     4501 atctggctaa acgattcggc ttgaaacaac ttggtatctc gggtatttct ccagagcaag
     4561 agccctctcc tcgccaattg aaagaaattc aagactttgt caaagaatac aacgtcaaga
     4621 ctatttttgc agaagacaac gtcaatccca aaattgctca tgctattgcg aaatcaacag
     4681 gagctaaagt aaagacatta agtccacttg aagctgctcc aagcggaaac aagacatatc
     4741 tagaaaatct tagagcaaat ttggaagtgc tctatcaaca gttgaagtaa aggagattat
     4801 tagtgaagaa aacatatggt tatatcggct cagttgctgc cattttacta gctactcata
     4861 ttggaagtta ccaacttggt aagcatcata tgggtctagc aacaaaggac aatcagattg
     4921 cctatattga tgacagcaaa ggtaaggcaa aagcccctaa aacaaacaaa acgatggatc
     4981 aaatcagtgc tgaagaaggc atctctgctg aacagatcgt agtcaaaatt actgaccaag
     5041 gctatgtgac ctcacacggt gaccattatc atttttacaa tgggaaagtt ccttatgatg
     5101 cgattattag tgaagagttg ttgatgacgg atcctaatta ccgttttaaa caatcagacg
     5161 ttatcaatga aatcttagac ggttacgtta ttaaagtcaa tggcaactat tatgtttacc
     5221 tcaagccagg tagtaagcgc aaaaacattc gaaccaaaca acaaattgct gagcaagtag
     5281 ccaaaggaac taaagaagct aaagaaaaag gtttagctca agtggcccat ctcagtaaag
     5341 aagaagttgc ggcagtcaat gaagcaaaaa gacaaggacg ctatactaca gacgatggct
     5401 atatttttag tccgacagat atcattgatg atttaggaga tgcttattta gtacctcatg
     5461 gtaatcacta tcattatatt cctaaaaagg atttgtctcc aagtgagcta gctgctgcac
     5521 aagcctactg gagtcaaaaa caaggtcgag gtgctagacc gtctgattac cgcccgacac
     5581 cagccccagg tcgtaggaaa gccccaattc ctgatgtgac gcctaaccct ggacaaggtc
     5641 atcagccaga taacggtggc tatcatccag cgcctcctag gccaaatgat gcgtcacaaa
     5701 acaaacacca aagagatgag tttaaaggaa aaacctttaa ggaactttta gatcaactac
     5761 accgtcttga tttgaaatac cgtcatgtgg aagaagatgg gttgattttt gaaccgactc
     5821 aagtgatcaa atcaaacgct tttgggtatg tggtgcctca tggagatcat tatcatatta
     5881 tcccaagaag tcagttatca cctcttgaaa tggaattagc agatcgatac ttagctggcc
     5941 aaactgagga caatgactca ggttcagagc actcaaaacc atcagataaa gaagtgacac
     6001 atacctttct tggtcatcgc atcaaagctt acggaaaagg cttagatggt aaaccatatg
     6061 atacgagtga tgcttatgtt tttagtaaag aatccattca ttcagtggat aaatcaggag
     6121 ttacagctaa acacggagat catttccact atataggatt tggagaactt gaacaatatg
     6181 agttggatga ggtcgctaac tgggtgaaag caaaaggtca agctgatgag cttgctgctg
     6241 ctttggatca ggaacaaggc aaagaaaaac cactctttga cactaaaaaa gtgagtcgca
     6301 aagtaacaaa agatggtaaa gtgggctata tgatgccaaa agatggtaag gactatttct
     6361 atgctcgtga tcaacttgat ttgactcaga ttgcctttgc cgaacaagaa ctaatgctta
     6421 aagataagaa gcattaccgt tatgacattg ttgacacagg tattgagcca cgacttgctg
     6481 tagatgtgtc aagtctgccg atgcatgctg gtaatgctac ttacgatact ggaagttcgt
     6541 ttgttatccc acatattgat catatccatg tcgttccgta ttcatggttg acgcgcgatc
     6601 agattgcaac agtcaagtat gtgatgcaac accccgaagt tcgtccggat gtatggtcta
     6661 agccagggca tgaagagtca ggttcggtca ttccaaatgt tacgcctctt gataaacgtg
     6721 ctggtatgcc aaactggcaa attatccatt ctgctgaaga agttcaaaaa gccctagcag
     6781 aaggtcgttt tgcaacacca gacggctata ttttcgatcc acgagatgtt ttggccaaag
     6841 aaacttttgt atggaaagat ggctccttta gcatcccaag agcagatggc agttcattga
     6901 gaaccattaa taaatctgat ctatcccaag ctgagtggca acaagctcaa gagttattgg
     6961 caaagaaaaa tactggtgat gctactgata cggataaacc caaagaaaag caacaggcag
     7021 ataagagcaa tgaaaaccaa cagccaagtg aagccagtaa agaagaaaaa gaatcagatg
     7081 actttataga cagtttacca gactatggtc tagatagagc aaccctagaa gatcatatca
     7141 atcaattagc acaaaaagct aatatcgatc ctaagtatct cattttccaa ccagaaggtg
     7201 tccaatttta taataaaaat ggtgaattgg taacttatga tatcaagaca cttcaacaaa
     7261 taaaccctta accaaaagaa gatctcattg tcaaagcaag ttacgacggt gattttgaag
     7321 tcattctatg taacgagtag tgataaaagt tggataatag cggttttctt ttgcaaagaa
     7381 atggtatcca tgttagaata gtaaaaaaag aggaggattc ttggactaat gtcaaataag
     7441 tagacagaaa actgtgttat tttattgcgt taaaataatt ttcttctttc tgattagggg
     7501 ttagtcctag attagccgta tgtgggttgt aattgttata aaaattctca atgtattcaa
     7561 agcagtctaa ttgaacctgt ttgatatttt gataatgttt tcggttgatt tgtctatgct
     7621 ttaaatactt gaaaaatgct tcagttacgg cattatcata aggatatcca ggattagaaa
     7681 aagaatgcat gatattggca ctgcacccta atagtgagac gcaagaaaaa cacttttagg
     7741 caatcagttt tctgtactgt acaggcgact ggtcgtttaa tctctgttga attctagttt
     7801 cattataaaa tgtaatgtaa tttttaacaa tatttgttat actatctttg ttgtattttc
     7861 tcctattatg gaaataaaag gtttcagtct ttaggacggt gtgaaaccat tcaatacagg
     7921 cattatctgc aggtgttcct tttcgagaca ttgagcggat aatgtctttt tccgtgcaag
     7981 cctggtagta agccatagaa gtatacactg agccttggtc actgtgtaag attgctcctt
     8041 tatttaggca attttaactg attaagggtg tctagtacaa aatccgtgtc ctgacaatct
     8101 gagatagtgt aagctataat ttctcggtta tagagatcca taattgatga gagatacaat
     8161 ttacagttac cgaaatatag gtaggtaata tctgttacga gcttttcctt aggcttatcg
     8221 gcatggaaat cccgactcaa tttattatct gttaaataat aagctttacc caaattggga
     8281 actttcttgg tacgtgtccg acaaagccag ccattatttt tcatgatacg atagactttc
     8341 tttgtattaa cagtcaatcc gtggattttt ttgagcaatc gtgtaatggt acgatagcca
     8401 taaataaagt gattctccat acagagctgt tcaattaatt caataaggtc atcttttttt
     8461 gcggcttctc atactccttt ttccaacggt aataggtcga ccgcttgacc ttaaaacagt
     8521 ctagaatgaa aactatcggg tagttgtttt tatagtcttc cacaagcttg ataagactta
     8581 ctttatcgat ttccttatca agcctcgata cttttttaag aggtcaacct gtaattgtaa
     8641 ttgttccact tcagacagat gttccaagcc tttaccgtag gtatattgct tgccaacacc
     8701 ttgatgaaaa cgataaagct cctcgttttc gtaccatttc atccaagtat agatttgact
     8761 attatttttg atgcctaaag tctccataat aactctgtta gacttgcctg ctttcttcat
     8821 atcgatgcaa gccagcttag tttcccatga atatgctttt ttaaccataa taaaacactc
     8881 ctgtttctag tttactaaat ttcaacagga gtgtttttct tttgtctcat tttagggatt
     8941 cagtgcctat tgttgtcatc aattattttt ctaaattccc cggacttaaa ttgtgaccct
     9001 tggtcggaat gaaagagaag tgttccttca atctttcttt tattaagtga aaaggcaaca
     9061 cttttctgta caacatttat aaagtgtttt tctaggcaat taatctttta gtcattggtg
     9121 tttggtagtt gagactacca tgaatgcggt ggtaattcca ccaatgaaca tagtctttag
     9181 tcttaagagc tagttcttcc agcaattgaa aggtttcttg ataaacaaat tcaattttga
     9241 aagcacgata cgtactttca gctacggcat tgtcataagg acaaccagcc tgactaagcg
     9301 aacgtgtgat tccaaaggct tccaatattt catcaattaa ctgattatca aactctttgc
     9361 cacgatctga atggaacatc ttgactttgg tcagggcgta agggatgctt tgtatggctt
     9421 gcttaacgag ttcagcggtc ttgtgccaac caagagacag gccgatgatt tcacggttgt
     9481 ataggtcaat gatgaggcaa acataagccc aacgattgcc tgcacgaaca taggttaagt
     9541 cagtgactaa ggc
//
LOCUS       DATRNK010000066         8695 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.066, whole genome shotgun sequence.
ACCESSION   DATRNK010000066 DATRNK010000000
VERSION     DATRNK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8695
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.066"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            82..756
                     /locus_tag="VBC52_001695"
     CDS             82..756
                     /locus_tag="VBC52_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO6883259.1"
                     /translation="MDKEKLDYWKTIITFLHNVLGDNYEIVLHVVDENDIYIGELVNS
                     HISGRTISSPLTTFALDLIKNKVYKEKDFVTNYKAIVSPLNKEVRGSTFFIKNAQNEL
                     EGMLCINLDISAYQNIALDILDLVNLNVNKILPKSPQKISLPQQEEPVEVLSGNIQDI
                     ISEIVDPSLLNQNIHLSQEVKVEIVSKLHEKGVFQLKGAVSKVAEVLNISEPSVYRYL
                     KKIETH"
     gene            926..3178
                     /gene="gshAB"
                     /locus_tag="VBC52_001696"
     CDS             926..3178
                     /gene="gshAB"
                     /locus_tag="VBC52_001696"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEO6883260.1"
                     /translation="MIIDQLLQRSHSHLPILQATFGLERESLRIHQPTQRVAQTPHPK
                     TLGSRNYHPYIQTDYSEPQLELITPIAKDSQEAIRFLKAISDVAGRSINHDEYLWPLS
                     MPPKVREEDIQIAQLEDAFEYDYRKYLEKTYGKLIQSISGIHYNLGLGQELLTSLFEL
                     SQADNAIDFQNQLYMKLSQNFLRYRWLLTYLYGASPVAEEDFLNQKLNNPVRSLRNSH
                     LGYVNHKDIRISYTSLKDYVNDLENAVKSGQLIAEKEFYSPVRLRGSKACRNYLEKGI
                     TYLEFRTFDLNPFSPIGITQETVDTVHLFLLALLWIDSSSHIDQDIKEANRLNDLIAL
                     SHPLEKLPNQAPVSDLVDAMQSVIQHFNLPPHYQDLLESVKRQIQSPELTVAGQLLEM
                     IEGLSLETFGQRQGQIYHDYAWEAPYALKGYETMELSTQLLLFDVIQKGVNFEVLDEQ
                     DQFLKLWHNSHIEYVKNGNMTSKDNYIVPLAMANKVVTKKILDEKHFPTPFGDEFTDR
                     KEALNYFSQIQDKPIVVKPKSTNFGLGISIFKTSANLASYEKAIDIAFAEDSAILVEE
                     YIEGTEYRFFVLEGDCIAVLLRVAANVVGDGIHTISQLVKLKNQNPLRGYDHRSPLEV
                     IELGEVEQLMLEQQGYTVNSIPPEGTKIELRRNSNISTGGDSIDVTNTMDPTYKQLAA
                     EMAEAMGAWVCGVDLIIPNATQAYSKDKKNATCIELNFNPLMYMHTYCQEGPGQSITP
                     RILAKLFPEL"
     gene            3302..4120
                     /locus_tag="VBC52_001697"
     CDS             3302..4120
                     /locus_tag="VBC52_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883261.1"
                     /translation="MVKKRYSKNSHNLITLLGIVVLASLISDFWSEVIATLLIIGGGY
                     CAYYVYDKKRLKRFTSNQRIEALKSNIKETDQDIRHLEILKKDNLSKEYIKLANQILP
                     QLDLIRNEANQLQKAIEPNIYKRITKKANTFSNEINEQLIKLHASPELEPISDQEDEM
                     IRIAPELKPFYHNIQDDHYAILKKIEKADNKAELAAIHQANMKRFTDVLAGYIRIKQS
                     PKNFNNAKERLEQALQAIKKFNLDLDETLRQLNESDMKDFDVSLRMMQDERNNK"
     gene            4117..5373
                     /locus_tag="VBC52_001698"
     CDS             4117..5373
                     /locus_tag="VBC52_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEO6883262.1"
                     /translation="MSDTFNFDIDQIADNAITKTDKTTEIISNQTTSQTGQIAFFEKL
                     TPAQKSAISEKTPALVDTFVGDQNALLDFGQSAVEGVNTTVNHILSEQKKIQIPQVDD
                     LLKNANRELNGFIAKYKDATPAELEKKPNLIQKLFKQSKTSLQEFYFDSQNIEQKMDM
                     MAANVVKQEDTLARNIVSAEMLIEDNTKSIENLVGVIAFIESSQAEAANRASHLQQEI
                     LALDSQTSEYQIKSNQLARMTEVINTFEQQHTEYVSRLYVAWATTPQMRNLVKVSSDM
                     RQKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGVTADAIVNANNAALQMLAETSKEAIP
                     MLEKTAQSPTVSIKSVTALAESLVAQNNGIIAAIDKGRKERAQLESAVIKSAETINDS
                     VKIRDKKIVEALLNEGKSTQEKVDES"
     gene            5513..6388
                     /gene="hslO"
                     /locus_tag="VBC52_001699"
     CDS             5513..6388
                     /gene="hslO"
                     /locus_tag="VBC52_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEO6883263.1"
                     /translation="MDKIIKSISTSGSFRAYVLDCTETVRTAQEKHQTLSSSTVALGR
                     TLIANQILAANQKGNSKVTVKVIGDSSFGHIISVADTKGNVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVVITDYGTGQPYTSTTPLITGEIGEDFAYYLTESEQTPSAVGLNV
                     LLDDEDKVKVAGGFMLQVLPGASDEEISRYEKRIQEMPSISSLLESENHIESLLSAIY
                     GEDDYKRLSEDSLAFYCDCSKERFEAALLTLGTKELQAMKDEDKGVEITCQFCNQTYY
                     FTEEDLEKIINDSIK"
     gene            6372..7349
                     /gene="dusB"
                     /locus_tag="VBC52_001700"
     CDS             6372..7349
                     /gene="dusB"
                     /locus_tag="VBC52_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEO6883264.1"
                     /translation="MTQLNSSFMIGKVEIPHRTVLAPMAGITNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQSNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPEKIYHIVKEVTSVLDIPLTVKMRTGWSDSSNAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLGKVAKAVTSIPFIANGDIRTVHDAK
                     FMIEEIGADAIMVGRGARSNPYIFTQINHFFETGEILPDLPFEKMLDVAEDHLTRLVN
                     LKGETIAVREFRGLAPHYLRGKSGAAKIRGAVSRAETLAEVQELFAGLR"
     gene            7470..8111
                     /locus_tag="VBC52_001701"
     CDS             7470..8111
                     /locus_tag="VBC52_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEO6883265.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGQHLGTDVFYEAVDNNPVLDLYYQ
                     DPQKYAFLLQIFFLNKRFQSIKEAYKANNNVLDRSIFEDELFLTLNYKNGNVTKTELD
                     IYKELLANMLEELEGMPKKRPDLLVYIDVSFDKMLERIDKRGRSFEQVDSNPELYDYY
                     KQVHSKYPEWYENYDVSPKIRIDGNKLDFVKNPEDLQHVLDTIDSELQKLDLL"
     gene            8125..8616
                     /locus_tag="VBC52_001702"
     CDS             8125..8616
                     /locus_tag="VBC52_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEO6883266.1"
                     /translation="MIIRPVLPSDAKELLAIYAPYVTDTTITFEYDIPSISEFTNRIK
                     NISKSFPYLVAEENGKILGYAYASTYYARAAYDWTCELSIYLDKDARSKKISSQLYDT
                     LEKELVSQGYVNLLACISLPNDISIAFHKKRGFNQVAHFPKIGFKFEQWHDIVWLQKR
                     LKS"
BASE COUNT         2990 a         1556 c         1530 g         2619 t
ORIGIN      
        1 atcttttaaa atagtctgat attgtctttt aaccataaat atattactat aatagtaaga
       61 ttagcataga ggaactacga tatggataaa gaaaaattag actattggaa gacgataata
      121 acgtttctcc ataatgtttt aggtgataat tacgaaattg ttttgcatgt agtagatgaa
      181 aatgatatct atattggtga gttggttaat agtcacatta gtggacgaac cataagttca
      241 cctctaacga ctttcgccct tgatttaatc aagaataaag tttataagga aaaagatttt
      301 gttactaact ataaggcgat tgtcagccct ctcaataaag aagttcgtgg atctactttc
      361 ttcatcaaga atgctcaaaa tgaacttgaa gggatgttat gtatcaacct tgatatttct
      421 gcataccaaa atattgcttt ggatattctg gacttagtta atttaaatgt taacaaaata
      481 ctacctaagt cccctcaaaa aatttctctc cctcaacagg aggaacctgt agaagttcta
      541 tcaggcaata ttcaggatat tattagtgag attgttgatc ctagtctttt aaatcagaat
      601 attcatttaa gtcaagaagt taaggtagaa attgtttcta aacttcacga aaaaggtgta
      661 ttccaattaa aaggtgccgt ttcaaaggtc gctgaagttc tcaacatctc cgaaccaagc
      721 gtctatcgtt atcttaaaaa aattgaaact cattaactct aaaactcttt ttaacttata
      781 gattaataag attggactca aaagttcaat cttttatttt ttatgacacc ggtataagat
      841 tattttagtt ttctgtgccg taaaacttag tttttattat ttttttgata aactagtatc
      901 atctgttact aggaggaatg ttgtcgtgat tatcgatcaa ctgttacaaa gaagccactc
      961 tcatctaccg attctacaag ctacatttgg cttagagaga gaaagccttc gcattcacca
     1021 acccactcaa agggttgctc aaacacccca tcccaaaaca ttagggagtc gtaactatca
     1081 tccttatatc caaacggatt atagtgaacc tcaattagaa ctcattacac ctattgcaaa
     1141 ggatagccaa gaagccattc gatttttgaa agctataagt gatgttgctg gacgctctat
     1201 caaccacgat gaatacttat ggccgctttc tatgccaccc aaagtgaggg aagaagacat
     1261 acagattgcc caattagagg atgcttttga atatgattat cgcaaatatt tggagaaaac
     1321 ttatggaaaa ctaatacaat ccatctctgg aattcactat aatctcggtt taggtcaaga
     1381 gttattaact tctctctttg aattgagtca agcagacaat gctattgatt ttcagaacca
     1441 gctttacatg aaattgtctc aaaatttttt acgttaccgt tggttactaa cttatctata
     1501 tggagcgagc ccggtagctg aagaagactt tttaaatcag aaactgaata accctgttcg
     1561 gtcacttaga aatagtcact taggttatgt gaatcataaa gatattcgta tttcttatac
     1621 tagcttgaag gattatgtca acgatctaga aaatgctgta aaaagtgggc aattgattgc
     1681 tgagaaagaa ttttattcac ctgttcgtct ccgtggtagt aaggcctgtc gtaattattt
     1741 agaaaaagga attacatatt tagaatttcg cacttttgat ctcaacccat ttagtccaat
     1801 cggtataacg caggaaactg ttgatactgt tcaccttttc ttattggcgc ttttgtggat
     1861 agattctagt agtcatattg accaagatat caaggaagcc aatagattaa atgaccttat
     1921 agcactcagt catccattag aaaaattacc taatcaagca ccagtttctg acttagtgga
     1981 tgctatgcaa tctgttatcc aacattttaa cttaccacct cactatcaag acctattgga
     2041 atctgtaaaa aggcaaattc agtcacctga attaacggta gctggtcaac ttttagaaat
     2101 gattgaagga ctttccttag aaacatttgg acaaagacaa ggacaaattt atcacgatta
     2161 tgcttgggaa gctccctacg ccttaaaggg atatgaaacg atggaacttt ctacccaatt
     2221 gctactattt gacgttatcc aaaaaggagt caacttcgaa gtgctggatg aacaagatca
     2281 attcctaaaa ttatggcaca atagtcatat tgagtacgtt aaaaatggta atatgacctc
     2341 aaaagataac tatattgtac cgctcgctat ggctaacaaa gttgttacaa aaaaaatcct
     2401 agatgaaaag catttcccaa ctccttttgg agatgaattt actgaccgta aagaggcact
     2461 taactatttt tcacaaatcc aagataaacc aatcgtcgtt aagccaaaat caacaaactt
     2521 tggtttaggc atttctattt ttaaaacatc agctaactta gcttcttatg aaaaagcaat
     2581 tgacatcgct tttgccgaag atagtgctat tcttgtggaa gaatatattg agggtaccga
     2641 ataccgtttc ttcgtccttg agggggactg tattgctgta ttgcttaggg tagcagctaa
     2701 tgtcgttgga gatggtattc atactattag tcagctagtt aaacttaaaa accaaaaccc
     2761 tctcagaggc tatgatcatc gctctcctct agaggttatt gagctcggag aagtagaaca
     2821 gttaatgttg gaacaacaag gatacactgt taacagtatc cctccagaag gaacaaaaat
     2881 agaacttcgc cgtaattcta atatttccac tggcggtgac tctatagatg taacaaatac
     2941 aatggatcct acatataaac aactagcagc tgagatggca gaggctatgg gggcttgggt
     3001 ttgtggagtt gatttaatta ttcctaatgc cactcaagca tactcaaaag ataagaaaaa
     3061 tgctacttgc attgaactaa actttaaccc attgatgtat atgcacacct actgtcaaga
     3121 ggggcctggt caatctatta cacctcgaat tttggctaaa ctgttcccag aattataact
     3181 aaaaagagtt ccttaggcta agcttaagga atttttggtt aaaataaagt tatcccttgt
     3241 catcactagt tcatttaaga taaaatatta acaactacag aaagttaggt taaatattat
     3301 tatggttaaa aaacgctatt ccaaaaattc tcataatcta ataacattac ttggtattgt
     3361 tgttcttgct tccctgattt cagacttttg gagtgaggtg attgctactc tgttaattat
     3421 tggtggtggt tattgtgcct attacgttta tgataaaaag cgtcttaaac gttttacatc
     3481 aaaccaacgt attgaagctt taaaatccaa tattaaagag actgaccaag acattagaca
     3541 cttggaaatt ttaaaaaaag acaatctttc taaggaatat ataaaattag caaatcaaat
     3601 attacctcaa ttggacctaa ttcgtaatga agcaaaccaa ctacaaaaag ccattgagcc
     3661 taatatctat aaacgtatca ccaaaaaagc aaatacattt agtaatgaga ttaacgaaca
     3721 actaataaag ctacatgcct ctccagaact agaacctatt tctgatcagg aagatgaaat
     3781 gatacgcatt gctccggaat taaaaccgtt ctatcacaat attcaagatg accactatgc
     3841 tatcttaaaa aagattgaaa aggcagataa caaagctgaa ttagcagcaa ttcatcaagc
     3901 caatatgaaa cgttttacag atgttctggc tggctacatc agaatcaaac aatctcctaa
     3961 aaattttaac aatgctaagg aacgcctaga acaagcatta caagcaatta aaaaatttaa
     4021 tttagattta gatgaaacgc tgcgccaatt gaatgaaagt gatatgaaag attttgacgt
     4081 tagcctaaga atgatgcaag acgaaaggaa caataaatga gcgatacctt taattttgat
     4141 attgaccaaa ttgcagacaa tgctatcact aaaacagata aaacaacaga aattatttcc
     4201 aaccagacaa caagccaaac tgggcaaatt gccttttttg aaaaactaac accagcacaa
     4261 aagtctgcta tctctgaaaa aacaccagct ttggtagata cttttgtcgg tgaccaaaat
     4321 gcgctccttg attttggaca atccgcagta gaaggcgtta ataccactgt taatcatatc
     4381 ttgtctgagc agaaaaaaat tcaaattcct caagttgatg atttactaaa aaatgctaat
     4441 cgcgaactaa atggatttat tgccaaatat aaagatgcta ctccggcaga attagagaaa
     4501 aaaccaaact tgattcaaaa attattcaaa caaagcaaga cctcgctaca ggaattttat
     4561 tttgactcac aaaacatcga gcaaaaaatg gatatgatgg cagcgaatgt tgtcaaacaa
     4621 gaagatactt tggcaagaaa tatcgtctct gctgaaatgc tcattgaaga taatactaaa
     4681 tctattgaaa atttggttgg agttattgct tttattgaat cgagtcaagc cgaggctgcc
     4741 aatcgtgcaa gccacttaca acaagaaatt ctagcattag atagccaaac gtccgagtat
     4801 caaattaaaa gtaaccaatt agctcgaatg actgaagtta tcaatacctt cgaacagcaa
     4861 catactgaat atgtcagccg tctctacgtt gcatgggcaa caacaccaca gatgcgaaac
     4921 ttggtcaaag tatcgtcaga tatgcgtcaa aaacttggca tgttacgtcg aaataccatt
     4981 ccaacaatga aactctcaat cgctcagtta ggcatgatgc aacaatctgt caaatccggt
     5041 gtcactgctg atgctattgt caacgctaat aatgcagcat tgcagatgct ggctgaaact
     5101 agtaaagaag cgattccgat gttagagaag accgcacaaa gccccactgt ttctattaaa
     5161 tctgtcactg cattagctga aagcttagtg gctcaaaata atggtattat cgctgccata
     5221 gacaaaggac gtaaagaacg tgcccaatta gaatctgctg ttattaaatc ggctgaaaca
     5281 atcaatgatt ctgtcaaaat tcgtgataaa aaaatagttg aagccttact caacgaaggt
     5341 aaatctaccc aagaaaaagt tgatgagtct taacttacta tcatatccac taaataaaaa
     5401 cgatactgta aaacaccgta tcgtttttat atcattaatt tataccgttt tcatctatat
     5461 tttcctgact atctccaatg caaaataagc cttttatgct ataatatgac ttatggataa
     5521 aattataaaa tcaatttcaa cttccggttc tttccgggct tatgtccttg attgtacaga
     5581 aactgtaaga acagctcaag aaaaacatca gacactttca agttctactg tagctcttgg
     5641 gagaacatta attgcaaatc aaattttagc agcaaatcaa aaaggaaata gtaaagtaac
     5701 tgtcaaggtt attggagata gctcttttgg tcatattatt tctgtagctg atactaaagg
     5761 aaatgttaaa ggttacatcc aaaatacggg tgttgatatc aagaaaacag ctactggaga
     5821 ggtcttagtt ggacctttca tggggaatgg acactttgtc gttattaccg attacggaac
     5881 tgggcaacct tacacttcaa caactccact aattacagga gaaattggag aagattttgc
     5941 ttattattta accgaaagtg agcaaacacc ttctgccgtc ggtttaaatg tcctcttaga
     6001 tgacgaggat aaggttaaag ttgcaggtgg attcatgctt caggtgctcc caggtgcttc
     6061 tgacgaagaa atttctcgct atgaaaaacg cattcaggag atgccttcta tttcaagtct
     6121 gctagagtct gaaaatcata tagaatctct cctttcagct atttacggag aagacgacta
     6181 caagcgctta tcggaagact ctttagcctt ctactgtgac tgttcaaaag aacgttttga
     6241 agccgctcta ttgactttag gaactaaaga attgcaagcc atgaaggatg aagataaagg
     6301 tgttgaaatt acttgtcaat tttgcaacca gacttactat ttcacagaag aagatttgga
     6361 gaagataatt aatgactcaa ttaaatagtt catttatgat tgggaaggtt gaaattcctc
     6421 atcgtaccgt actagcaccg atggcaggta ttactaattc tgcttttcgt actatcgcta
     6481 aagaatttgg tgctggtctc gttgttatgg aaatgatttc tgagaaaggg ttactttata
     6541 acaacgaaaa gaccttacat atgctccata tcgatgaaaa tgaacatcct atgtcaattc
     6601 aactatttgg tggcgatgca gaaggcttaa aacgtgcagc tgactttatc caaagtaata
     6661 cgaaagcaga tatcgtagac atcaatatgg gatgtccagt taacaaagtt gttaaaaacg
     6721 aagctggagc taaatggcta cgtgaccctg aaaaaattta tcatattgtt aaagaggtaa
     6781 cgtctgtact tgacattcca ttaacagtaa aaatgcgtac aggatggtct gactcatcta
     6841 atgctgtaga aaatgcactc gctgccgaat ctgctggtgt atctgcactt gctatgcacg
     6901 gtcgaacgcg tgaacaaatg tatactggca cgtgtgacca tgaaacactt ggcaaggtcg
     6961 ctaaagctgt tactagtatt ccttttattg ccaatggtga cattcgtact gttcacgatg
     7021 ccaaatttat gattgaagag attggagctg acgctataat ggttggacgt ggtgctcgta
     7081 gcaatcctta catctttaca caaattaatc atttctttga aacaggcgaa atccttcctg
     7141 acctaccatt tgagaagatg ttagatgttg ccgaggatca tctcactcga ctagttaatt
     7201 taaaaggtga aactattgcc gttcgtgaat ttcgagggtt agcacctcat tatctgcgtg
     7261 gaaaatcagg cgcagcaaaa attagaggag ctgtttcacg tgcagaaaca ctagcagaag
     7321 ttcaagaact ttttgcaggt ttacgctaaa tcaaaaacag tcctttgact gttttttcct
     7381 atctctgtac aaatttagca taaatcattg tgtaatgaaa actcaaaagt ataacaaatt
     7441 attttagcat aacagaatta aggagaatta tgttgattgt tttagctgga accattggag
     7501 caggtaaaag ctcccttgca gctgctctag gacaacacct agggacagat gttttctatg
     7561 aggcggttga taataaccca gttttagatc tttactatca agatccccaa aaatatgctt
     7621 ttttactaca aatattcttt ttaaataaac gattccaatc tataaaagaa gcctataagg
     7681 ctaacaacaa tgttttggat cgttctattt ttgaagatga actattcctc actctaaatt
     7741 ataagaatgg aaatgtgact aaaacagagc tagatatcta taaagaactt cttgcaaata
     7801 tgttggaaga attagaaggt atgcctaaga agagacctga tttgctagtc tacattgatg
     7861 tttcatttga taaaatgtta gaacgtattg acaaacgagg acgcagcttt gagcaagttg
     7921 attctaaccc agaattatac gactactaca aacaagtcca ttctaaatat ccagaatggt
     7981 atgaaaatta cgacgtttca cctaaaattc ggatcgatgg taacaaactt gatttcgtca
     8041 aaaatccaga ggatcttcaa cacgtccttg acactattga ttccgaattg caaaaacttg
     8101 atttactata attaggtgat atctatgatt ataagacctg tcctaccctc agatgctaaa
     8161 gaattacttg ctatctatgc tccctacgtt actgacacta caataacgtt tgaatacgat
     8221 attcctagta tatcggaatt caccaatcgt atcaaaaata tcagcaaatc tttcccatat
     8281 ttagttgctg aagaaaatgg gaaaatatta ggttatgcat atgcttcaac ttactacgct
     8341 agagctgcct atgactggac ttgcgagctt tctatttacc ttgataaaga tgcacgtagc
     8401 aaaaaaatta gtagccaact atatgataca ttggagaaag aattagttag ccaagggtat
     8461 gttaatctac tcgcttgtat cagtttacca aatgatataa gtattgcatt ccacaaaaaa
     8521 agaggattta atcaagtcgc acatttccca aaaatcggat ttaaatttga acaatggcat
     8581 gacattgtat ggttacaaaa gcgattgaaa tcttaaggta atctttagat ttattttaag
     8641 ttagcaaaag attaggatat tataatatat ttatcctaaa atttttcaac gactt
//
LOCUS       DATRNK010000067         8615 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.067, whole genome shotgun sequence.
ACCESSION   DATRNK010000067 DATRNK010000000
VERSION     DATRNK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8615
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.067"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            49..165
                     /locus_tag="VBC52_001703"
     CDS             49..165
                     /locus_tag="VBC52_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEO6883267.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
     gene            complement(257..1141)
                     /locus_tag="VBC52_001704"
     CDS             complement(257..1141)
                     /locus_tag="VBC52_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEO6883268.1"
                     /translation="MSLIILTVILVLVIVLLITSLYVVKQQTVAIIERFGKYQKTATS
                     GIHIRVPLGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNIATQYRVNENNVTDAYY
                     KLIKPEAQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIVKT
                     LITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVGIA
                     QQRKAIVDGLADSIQELKDANVTLTEEQIMSILLTNQYLDTLNTFAINGNQTIFLPNN
                     PEGVEDIRTQVLSALKTR"
     gene            complement(1289..2953)
                     /locus_tag="VBC52_001705"
     CDS             complement(1289..2953)
                     /locus_tag="VBC52_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEO6883269.1"
                     /translation="MSKITTSLFQEMVQAASTRLSKQAEYVNSLNVFPVPDGDTGTNM
                     GMTMENGAKEVSDKPATTVGEVGQILSKGVLMGARGNSGVITSQLFRGFGQSIKDKEE
                     LTGQDLAHAFQNGVEVAYKAVMKPVEGTILTVSRGAATAALKKAEETDDAVEVMRATL
                     KGAKRALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFKATPATM
                     TEMVNAEHHKAVVGHVATEDIKYGYCTEVMVGLKQGPTYVKEFNYEEFQGYLSNLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVENMRNQHDAQMQKVEVEETVK
                     ETKEYGIIAVVAGDGLAEIFKSQGVDYIISGGQTMNPSTEDIVKAIEKVNARNVIILP
                     NNKNIFMAAQSAADVVDIPAAVVETRTVPQGFTSLLAFDPAKSLETNVADMTNSLSDV
                     ISGSVTLAVRDTTIDGLEIHENDILGMVDGKILVSTPDMEKALKDTFDKMIDEDSEIV
                     TIYVGEDGKQALAETLSEYLEETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            complement(2953..3318)
                     /locus_tag="VBC52_001706"
     CDS             complement(2953..3318)
                     /locus_tag="VBC52_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6883270.1"
                     /translation="MTVKINTRDGQIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQALLRKENYAKGVVVRSSEAGIVVDVYTVMSYGVKISEVSKNIQERVKFNLENQLG
                     LSADMVNVYVQSIKVVGED"
     gene            complement(3450..3638)
                     /locus_tag="VBC52_001707"
     CDS             complement(3450..3638)
                     /locus_tag="VBC52_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEO6883271.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTVLIDGKP
                     KKVWVSARALKSGKVERV"
     gene            3877..4794
                     /locus_tag="VBC52_001708"
     CDS             3877..4794
                     /locus_tag="VBC52_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO6883272.1"
                     /translation="MTRKIGIIGMGHVGSTVAHALIAQGIGDDYVFIDTNVDKVNADM
                     IDFQDALANATYHANLVLNDYDALSDADVVISALGDISLQNNKEANRFAELPFTSKEV
                     YDVSQKLKSSGFRGILIVVTNPVDAITQLYQEYTQFPKSRVIGTGTLLDTARMKRVLG
                     QRLQVDPRSIGGYNLGEHGNSQFVAWSQVTVKGQPAISLLKAEELEQIKEASLKGGHT
                     VFFGKKYTSYGIATAAVRIVLTVLNDSHELLPVSTFYESEGVYLGYPAIIGRVGIVER
                     ISLSLSPKEEQQLTDSAAFIRNYLKHINL"
     gene            complement(4878..5759)
                     /locus_tag="VBC52_001709"
     CDS             complement(4878..5759)
                     /locus_tag="VBC52_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEO6883273.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKLCKQLIETLVESMGITVPVAIHLDHGHYDDALECIEVGYTS
                     IMFDGSHLPVEENLEKAREVVAKAHAKGISVEAEVGTIGGEEDGIVGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPANWEGLDLDHLKKLTEAVPGFPIVLHGGSGIPDDQI
                     QEAIKLGVAKVNVNTECQLAFCQATRDYAVEFNANEAEYMKKKLFDPRKFLKPGFDAI
                     TEAVEERIDVFGSANKA"
     gene            complement(5900..7288)
                     /gene="argH"
                     /locus_tag="VBC52_001710"
     CDS             complement(5900..7288)
                     /gene="argH"
                     /locus_tag="VBC52_001710"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEO6883274.1"
                     /translation="MTKNHKLWGGRFESSLEKWVEEFGASISFDQKLAPYDMKASMAH
                     VTMLGKTDIISQEEAGLIKDGLKILQDKYRAGQLTFSISNEDIHMNIESLLTAEIGEV
                     AGKLHTARSRNDQVATDMHLYLKDKLQEMMKKLLHLRTTLVNLAENHIYTVMPGYTHL
                     QHAQPISFGHHLMAYYNMFTRDTERLEFNMKHTNLSPLGAAALAGTTFPIDRHMTTRL
                     LDFEKPYSNSLDAVSDRDFIIEFLSNASILMMHLSRFCEEIINWCSYEYQFITLSDTF
                     STGSSIMPQKKNPDMAELIRGKTGRVYGNLFSLLTVMKSLPLAYNKDLQEDKEGMFDS
                     VETVSIAIEIMANMLETMTVNEHIMMTSTETDFSNATELADYLASKGVPFRKAHEIVG
                     KLVLECSKNGSYLQDIPLKYYQEISELIENDIYEILTAKTAVKRRNSLGGTGFDQVKK
                     QILLARKELKAE"
     gene            complement(7307..8497)
                     /locus_tag="VBC52_001711"
     CDS             complement(7307..8497)
                     /locus_tag="VBC52_001711"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEO6883275.1"
                     /translation="MGKEKLILAYSGGLDTSVAIAWLKKDYDVIAVCMDVGEGKDLDF
                     IHDKALTIGAIESYILDVKDEFAEHFVLPALQAHAMYEQKYPLVSALSRPIIAQKLVE
                     MAHQTGATTIAHGCTGKGNDQVRFEVAIAALDPELKVIAPVREWKWHREEEITFAKAN
                     GVPIPADLDNPYSIDQNLWGRANECGVLENPWNQAPEEAFGITKSPEEAPDCAEYIDI
                     TFQNGKPIAINNQEMTLADLILSLNEIAGKHGIGRIDHVENRLVGIKSREIYECPAAM
                     VLLAAHKEIEDLTLVREVSHFKPILENELSNLIYNALWFSPATKAIIAYVKETQKVVN
                     GTTKVKLYKGSAQVVARHSSNSLYDENLATYTAADSFDQDAAVGFIKLWGLPTQVNAQ
                     VNKG"
BASE COUNT         2589 a         1738 c         1379 g         2909 t
ORIGIN      
        1 gtatactggt gtctgttaac gttaataaat tgtctatagg aggtaatcat ggctgaaaaa
       61 acagacctat cgtcagccta tcgcagactt aaaagtccta acattaaaac tagaaaacgt
      121 gctctgaaaa tcattcatga gtacaaacgt tacggtaaaa aataactttt ttccgtctct
      181 ccaattataa taaataagtc agacacaaaa taaccagagc aaatgctctg gttattttgc
      241 ttctataaaa agttcattat cttgttttaa gagcagataa aacttgtgta cgaatatctt
      301 caacaccttc tgggttattt ggtaagaaaa tcgtttgatt accattaata gcaaatgtat
      361 ttaaagtatc taaatattga tttgttaaca agatagacat gatttgttct tctgtcaatg
      421 tcacgttagc atctttcaat tcttgaatcg aatcagctaa cccatctaca attgctttac
      481 gttgttgcgc aatacccaca ccatgtagac gatctttttc tgcttctgct tctgcagcag
      541 ttacaatttt gatcttatca gcattcgcta attcttgagc tgctacacgt ttacgttgtg
      601 cagcattgat ttcgttcatg gattgtttta cttctgcatc tggctcaacc ttagtaatta
      661 aagtcttaac aatgatgtag ccgtatgttg acatttcttc tgccacttga tgttgaactt
      721 ccaaggctat ttcatctttc ttttcaaata gctcgtctaa tgtaagttta ggtactgatg
      781 aacgcaaagc atcttcgata taagatttaa tctgagcttc tggctttatt aatttgtaat
      841 aagcgtctgt aacgttattt tcattgacac gatattgtgt agcaatattt aaagtgacaa
      901 aaacattatc ttttgttttt gtctcaacga taatttcact ctgtaaaaga cgtaactgca
      961 ctcgtgctgc aatcttatcg atacccaatg gtacacgaat atgaataccg ctagtagcag
     1021 tcttttgata tttaccaaag cgttcaatga tggctactgt ctgttgttta acaacgtata
     1081 aagaagttat caacagaaca atcactaaaa ccaaaataac cgtaagaata attaatgaca
     1141 tcataaatct cctttgtatg aatgttttaa aacaattcta acacaatttg tcaaaatttt
     1201 caaaatactg atgctttatc atactaaaat agaaactctg ataaaataaa aaattagaag
     1261 tatctctcct aattttttat tttatcaatt attcaacgct catgaggtat ggatatacag
     1321 gttgatctcc ttggtggatc tcaacttcaa catcttcata agtttcctca agatattccg
     1381 acaaggtctc agctaatgct tgcttaccat cttcaccaac atagattgtc acgatttcac
     1441 tatcttcatc aatcatttta tcaaaagtgt ctttaagtgc tttttccata tctggtgtag
     1501 atactagaat tttaccatca accatcccaa ggatgtcgtt ttcatgaatt tcaaggccat
     1561 ctatcgttgt atcacgaaca gctaaagtca cactaccact aatgacatct gagaggctat
     1621 ttgtcatatc agctacatta gtttcaagag acttagcagg atcaaaagca agtaagctag
     1681 taaatccttg tggaactgta cgtgtctcta ctactgctgc tggaatatca acgacatctg
     1741 cagctgactg tgcagccatg aagatgttct tattgtttgg taaaataata acatttctag
     1801 cgtttacttt ttcaatcgct tttacaatat cttctgtaga tgggttcata gtctggccgc
     1861 cagagatgat ataatcaaca ccttgtgatt taaaaatctc agctagaccg tcaccagcta
     1921 caacagcaat aataccatat tctttagttt ctttgacagt ttcttcaact tcaacctttt
     1981 gcatttgggc atcatgttgg ttacgcatgt tttcaacttt aaccttcaca agcgatccat
     2041 atttcaaacc ttcttgcata acaagacctg gatcttctgt atgaacatga accttgacaa
     2101 tttcatcatc attaacaact agtaaagaat cccctagatt gctaagataa ccttgaaatt
     2161 cttcgtagtt aaattcctta acgtaagttg gtccttgctt taaaccaacc atgacttctg
     2221 tacaatagcc atatttaata tcttctgtgg ctacgtggcc tactacagct ttatggtgct
     2281 ctgcattaac catttcagtc attgttgcgg gagttgcttt aaaatcttct gatgcgatat
     2341 attccccagt cagtgctgag aggaaacctt cgtaaataaa gacgagtcct tgaccaccag
     2401 aatcaacaac accaacctct ttaagaacag gtagcatatc cggtgtttta gcaagagcac
     2461 gcttagcacc tttgagagtt gcgcgcataa cttcaactgc atcatcagtt tcttcagctt
     2521 tttttagagc agcggtagct gcaccgcgtg atactgtcaa gatagttcct tcaactggtt
     2581 tcataacggc tttataagct acttcaacac cattctggaa agcgtgtgcc aaatcttgac
     2641 ccgttaattc ttctttatcc ttgatacttt gaccaaaacc acggaataat tgtgatgtaa
     2701 ttacacctga atttccacgt gctcccatca atacaccttt agaaagaatt tgaccaactt
     2761 ctccaacagt cgtcgctggt ttgtccgaaa cttccttggc tccgttttcc atagtcatcc
     2821 ccatattcgt accagtatca ccatctggaa ctgggaatac atttaatgag ttgacatact
     2881 ctgcctgttt actcagacgt gtactggcag cttgtaccat ttcttggaat aaacttgtcg
     2941 taatctttga cactaatctt ctcctacaac cttaatactt tgcacataaa cattcaccat
     3001 atcagcagat aaaccaagct gattttcgag attgaatttc actcgctctt gaatattttt
     3061 tgaaacttca ctaattttga cgccgtaact catgacagta taaacatcta ctacgatgcc
     3121 cgcttcacta gacctcacaa caacaccttt tgcgtagttt tccttacgca aaagtgcttg
     3181 aaagttatct ttgatagcac ttttacttgc cataccaaca acgccaaaaa tttccgttgc
     3241 tgaacctcca acgacagttg caataacgtc atcagagagt tcaatttgac catctcttgt
     3301 attgattttt acagtcatat ctactacctc aaaaagtttt tatcactcta ttctatcata
     3361 tctcactatt attgtaaaaa aatagaggca tctaactatc aaatcagcct tagaaacact
     3421 aaaaaaagcc ctaaggcttt ctcagtttat taaacgcgtt ctactttacc agatttaagc
     3481 gcacgagctg aaacccaaac tttttttggt ttaccgtcga taagaacagt aactttttga
     3541 aggtttggtt taacagtacg ttttgtttgg ttcatcgcgt gtgaacggtt gttaccagaa
     3601 actgttttac gtcctgtgaa ataacatact ttagccattt tacgagttcc tcctatattg
     3661 atcaatattt acggatgtgc tagcaccaca tactagacta ttctatcaca acttacttct
     3721 tctagcaagt tcttttcaaa attacttttt catttgtttt atctaaatcg cttacatatt
     3781 ttttataata agacacactt ccttctaatt cattttttag tttacatttt ctttcaaaat
     3841 attataatag acaacaataa tcaaaggagg tttcatatga cacgtaaaat tggaattata
     3901 ggcatggggc acgttggatc aactgttgca cacgctctta ttgcacaagg tattggtgat
     3961 gattatgtct ttattgatac taatgttgat aaggttaatg ctgatatgat tgattttcaa
     4021 gatgctctgg caaatgctac ttatcacgct aatctagtat taaacgacta tgacgcgttg
     4081 tcagatgctg atgttgtgat ttcagcctta ggtgatattt cactacagaa taacaaagaa
     4141 gctaatcgct ttgctgaact accatttacc agtaaagaag tctacgacgt tagccaaaag
     4201 ttaaaatcat ctggttttag agggatcctt attgtcgtta ctaatcctgt tgatgccatt
     4261 acccagcttt atcaagaata tactcaattt cctaaatcac gcgttatcgg aacaggaacc
     4321 ttattggata ctgctcggat gaaaagagtg cttggtcaac gcctacaagt tgatccaagg
     4381 agtatcggtg gttataatct aggagagcat ggtaactccc aatttgtcgc ttggagtcag
     4441 gtaactgtaa aaggacaacc agctatatca ctattaaaag cagaagaatt agaacagata
     4501 aaagaagctt ctttaaaagg cgggcacact gtcttttttg gtaaaaaata tactagttat
     4561 ggtatcgcta ccgcagctgt ccgcatcgtt ttaactgtgt taaatgattc ccatgagtta
     4621 ctacctgtat ctaccttcta tgagtccgaa ggggtttacc ttggatatcc agctattatt
     4681 ggtagagtag gaattgtaga aagaatttct ctttcactaa gcccaaaaga agagcaacaa
     4741 ctcacagatt cagcggcatt tattcgaaat tatcttaagc atatcaatct ttaaagagta
     4801 tctatttaat aaaaagtcat ataatccaaa aaaagctcac cataagtgag cttttaacta
     4861 ttatctgttt atgttaatta tgctttgttt gctgaaccaa acacgtcaat acgttcttca
     4921 actgcttctg taattgcatc gaaaccaggt ttcaagaatt tacgtgggtc aaagagtttt
     4981 ttcttcatgt attcagcttc gttagcgttg aattcaacag cgtagtcacg agttgcttga
     5041 cagaaagcta attgacattc tgtgttaacg ttaactttag caacaccaag tttgatagct
     5101 tcttggattt gatcatcagg aatacctgaa ccaccgtgta acacgattgg gaaaccagga
     5161 acagcttcag tcaatttctt caagtgatca aggtcaagtc cttcccagtt tgctgggtat
     5221 ggtccatgaa tgttaccgat acctgcagcc aagaagtcaa taccagtttc aaccatagct
     5281 ttagcgtctt cgattggtgc taattcacct ttaccgacaa taccatcttc ttcaccaccg
     5341 atagtaccaa cttcagcttc tactgaaata cctttagcat gtgctttagc aacaacttcg
     5401 cgtgcttttt caaggttttc ttcaactgga aggtgtgaac catcaaacat aattgaagtg
     5461 taacctactt caatacactc aagggcatca tcgtaatgac cgtggtcaag gtggatagca
     5521 actggtacag taatacccat tgattcaacc aaagtttcaa taagttgttt acaaagtttg
     5581 taaccaccca tgtatttagc cgcacccatt gaagtttgga taagtactgg agctttttta
     5641 gcttctgctg cgcgcaagat agcttgagtc cactcaaggt tgtttgtgtt aaatccacct
     5701 actgcatagc cgttatcacg agctgcttgg acgaattttt ctgctgaaac gattgccatt
     5761 attcaggcct cctatttatt ttttgggcgt tgcccactta catgttaatt ctagcacaat
     5821 tctataaaaa atgctagttt tttactgaaa atacgtgaga aacttaacag agttaaacga
     5881 gggttttttc agaaagcagt cattccgctt tcaattcttt acgtgccaat aggatctgtt
     5941 ttttaacctg gtcaaacccc gttcctccta atgaatttct tcgtttaact gctgtctttg
     6001 cagttaatat ctcatagata tcgttttcta tcaactctga aatttcttga taatatttta
     6061 aaggtatatc ctgtaaataa gaaccatttt tactgcattc taaaaccaat ttacctacaa
     6121 tttcatgtgc ttttctgaaa ggtaccccct tacttgctaa ataatctgct aattctgttg
     6181 catttgaaaa atcagtctcc gttgaggtca tcattatgtg ctcattcaca gtcattgtct
     6241 caagcatatt agccatgatt tcaatagcta tgctgactgt ttctacgcta tcaaacatcc
     6301 cttctttatc ttcttgtaaa tctttgttat aagctaacgg caatgatttc ataacagtta
     6361 gtaaactaaa taaatttcca taaacacggc ctgtcttccc tcgaattaat tctgccatat
     6421 caggattttt cttttgaggc ataatagatg atcctgtaga gaaagtatct gataatgtga
     6481 tgaattgata ttcataactg caccaattaa tgatttcttc acaaaaacgt gataaatgca
     6541 tcattaaaat gctagcattg gataaaaatt ctatgataaa gtcacgatct gagacagcat
     6601 caagcgaatt actatatggt ttttcaaaat ctaataaacg agttgtcata tggcggtcaa
     6661 taggaaacgt tgtccctgct agagcagcag ctcctaatgg tgatagattg gtgtgtttca
     6721 tattaaactc taatcgctct gtatcacgcg taaacatatt atagtaagcc attagatgat
     6781 gtccaaatga aatcggctga gcatgttgta aatgtgtata ccctggcata acagtataga
     6841 tatgattttc tgctaaatta actaaggttg ttcgtaaatg aagcaatttc ttcatcattt
     6901 cttgcaattt atctttcaaa taaaggtgca tatctgtagc cacttgatca tttcgtgaac
     6961 gtgcagtatg aagcttacct gctacttctc caatttcagc ggttaataag ctctctatgt
     7021 tcatatgaat gtcttcattt gatatgctaa aagtcaattg acctgctctg tatttatctt
     7081 gcagtatttt taaaccatct tttatcaagc ctgcttcttc ttgactaata atatccgttt
     7141 tgcctaacat tgtgacatgg gccatagaag ccttcatatc atagggagct aatttttggt
     7201 cgaaactgat tgatgcaccg aactcttcaa cccatttttc aagactactt tcaaaacgac
     7261 caccccataa cttatgattt tttgtcatcc tcttatcttc ctttatttat cctttgttta
     7321 cttgggcatt aacttgggtt ggaagtcccc agagtttgat aaaaccaact gctgcatctt
     7381 gatcaaagct atcagcagct gtataggttg ctaaattctc atcatataat gaattagatg
     7441 agtggcgagc aacgacttgg gcagaacctt tatataactt aacttttgta gtaccattaa
     7501 ccactttttg tgtttctttg acataggcaa tgatagcttt tgtagctgga ctaaaccata
     7561 aagcattata aataagattt gatagttcat tttcaaggat aggtttaaaa tgagacactt
     7621 cacgaactag tgttaaatct tcaatttctt tatgagcagc taacagtacc attgccgcag
     7681 gacattcata aatttcacgt gatttgatac cgactaaacg attttcaaca tgatcaatgc
     7741 gtccaatacc atgttttcct gcaatttcat ttagcgataa aatcaaatct gctagtgtca
     7801 tttcttgatt gtttatagct ataggcttcc cattttggaa cgtaatatca atatattctg
     7861 cacaatcagg agcttcttct ggtgattttg taataccaaa agcttcttct ggcgcttgat
     7921 tccaaggatt ctcaagcaca ccacattcat tggcacgtcc ccataaattt tggtctatag
     7981 aataagggtt atctaaatct gctggaatag gtacgccgtt agcttttgca aaagtaattt
     8041 cctcttcacg atgccacttc cactcacgaa caggggcaat tacttttaat tcaggatcta
     8101 gtgctgcaat tgcaacttca aaacgcactt ggtcatttcc tttacctgta cagccatgtg
     8161 caattgtggt cgcacctgtt tgatgtgcca tttcaactaa tttttgggca ataattggac
     8221 gactaagcgc tgaaacaagc gggtattttt gttcatacat cgcatgtgcc tgtaaagctg
     8281 gtaaaacaaa atgctcagcg aactcatctt taacatctaa aatataagat tctatggcac
     8341 ctattgttaa agccttatcg tgaataaaat caagatcttt accttcgcca acatccatac
     8401 agacagcaat aacgtcataa tcttttttta accaagctat agcaacagag gtatctaaac
     8461 caccagaata agctaaaatt aatttttctt ttcccataaa aaatcccctt ccaaattcct
     8521 atgttttgta atattataca cttgctatat ttattgtcaa atatttttat agtttttgtc
     8581 tatttttagt atatttattt ctaaacaaat aaaac
//
LOCUS       DATRNK010000068         8575 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.068, whole genome shotgun sequence.
ACCESSION   DATRNK010000068 DATRNK010000000
VERSION     DATRNK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8575
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.068"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..209
                     /gene="essA"
                     /locus_tag="VBC52_001712"
     CDS             <1..209
                     /gene="essA"
                     /locus_tag="VBC52_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HEO6883276.1"
                     /translation="GQSDRKKRIKQLFSEESNQFKFVTRTEEATVSADIGHMIMYLVL
                     ISLVLILASIMTFKIKDKKDEYTY"
     gene            193..435
                     /locus_tag="VBC52_001713"
     CDS             193..435
                     /locus_tag="VBC52_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HEO6883277.1"
                     /translation="MNTHINITLDMGETSKDLRIPLKISVKQLVMELDSIFSRTERRT
                     KYQLRVINKGILLGENDILAHHPVTTGDRIRIEEFY"
     gene            435..1712
                     /gene="essB"
                     /locus_tag="VBC52_001714"
     CDS             435..1712
                     /gene="essB"
                     /locus_tag="VBC52_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HEO6883278.1"
                     /translation="METDSFVFEEQDFQFEKGPDFWQLSLKRSKVGTQNLQQLQLLEI
                     HHSLLMPMTTAIDADTIQFQFQTEAHALSFESFKKETLSEKLRLALNVLDLDKALSLP
                     VNFILHPSNLFLTKNATAKIAYRSLPGIMTPEKFGPEEFLYQFKCFVFALLTQHDYIE
                     LYNGAISVIEVSDFLKSIYHAETIQAVRDIITTDYEQQVEVETHTLAKVSKAKYKLYK
                     YISVWLGALSTILLIPLVYLVFIHNPFKEKMLAADTSFIKVDYNQVINRLEHVKVSKL
                     PYTQKYELAYSYINGMSFSEEQREVILNNVTLKTDELYLDYWINIGRGLDDDAIDAAK
                     RLDDSDLVIYAIVQKMDQVRKDNSLSGKDREQKLSELQTDYDKYWKDRKTALTDEESK
                     SKNSNNHSTNSNKELSSEPSSTTTSTSSKTKSR"
     gene            1719..6128
                     /gene="essC"
                     /locus_tag="VBC52_001715"
     CDS             1719..6128
                     /gene="essC"
                     /locus_tag="VBC52_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HEO6883279.1"
                     /translation="MLVICYYQSLRYEFNIEEEKSFLISSNGKSPIPVSELENDITLK
                     NMQGQLVYIIDQKEKELTNGVEISGIVFYLANNQKEIYTPLDYEDILIGDKEGYHVRF
                     KEGAPNLLLKKIESNWQLNLFEGDIYLNNHLQKVVQQLPLSLGDEISFQGTIVKLFPD
                     EIQIWGGTDYETSLTKKVMSAYQFYAGYPDFHRSPRIIYRSSEDKITVNAPGNEPNKS
                     KDELLKLIVPPLVMIGVSILISIFRPRGIYIIATMSMALVTMIFSITGYFKNRKQYKQ
                     DLQERIDSYHDYLSDKSIELQKLAKEQKRGQHYHYPTIEGLQEMADTYHHRIYEKTPL
                     HFDFLYYRLGLGEVPTSYNIHYSQPERSGKKDPLENEGYNLYFNNRYIKNMPIVANLS
                     HGPVGYIGPRGLVLEQLQLMVNQLAFFHSYHDVQFITIVPEEEMDKWSWMRWLPHATL
                     QDVNVRGFVYNQRSRDQVLNSLNQILKLRRTQREDKSAKEGTLFSPHYVVIVTDEKLI
                     LDHVIMEFFTEDPTELGCSLIFVQDVMSSLSENIKTIINIKDRNTGQLVIEEGELKET
                     DFELDHFLEDYDKENISRRLAPLNHLQNLKSSIPEAVTFMEMYQAEEFEDLHVQERWI
                     SHAPYKSLAVPLGLRGQDDIVYLNLHEKAHGPHGLVAGTTGSGKSEIIQSYILSLAVN
                     FHPHDVAFLLIDYKGGGMANLFKDLPHLLGTITNLDGAQSMRALVSINAELKRRQRLF
                     AKADVNHINQYQKKYKLGEVSEPMPHLFLISDEFAELKSNQPEFMKELVSTARIGRSL
                     GIHLILATQKPSGVVDDQIWSNSRFKLALKVADRGDSMEMLHTPDAAEITQAGRAYLQ
                     VGNNEVYELFQSAWSGADYQPEKDDQGIEDHTIYSINDLGQYEILNDDLSGLDQAENI
                     KEVPTELDAIVENIQALTKEMGISDLPQPWLPPLSNQISVTDLRKEESVDLWSKAPSY
                     KAVLGFMDIPSQQAQEVAYHDFEDDGHLSIFAGPSMGKSTALQTVTMDLARHNSPEFL
                     NLYLFDFGTNGLLPLRRLPHVADFFTIDDDEKIAKFIARIKVEMSDRKKALSRYNVAT
                     AKLYRQVSGETMPQILIVIDSYEGLREAQTLTNLEACFQNISRDGSSLGISLVISAGR
                     MAALRSSLMANLKERIALKLTDDSESRTLVGRHQHIMEDIPGRGLIKRDDIEVLQVAL
                     PTEGTETFDIISNIQTESDAMNDKWTGPRPKAIPIVPEELTFDDFMATDSVQADLSAN
                     RLPLGLEMVDVESFSLPLTKFKHLLFLSDSDEGLENLGSHLLKTLIKVPEYSTMIIDS
                     LGEHEAYQGQVRTYVGADMISDMAEQLNYELGKRQEQNAFDRWFILIPDFESFVSKTN
                     LSLEQIQNLLDNGPKVGLHLIIGSAFSFVGSKLDPVNKYVKTNSQYVMLGMRLMDQTF
                     LEKVYNSKEARLERDEAYIHDRKNYQKLKLSID"
     gene            6141..6476
                     /locus_tag="VBC52_001716"
     CDS             6141..6476
                     /locus_tag="VBC52_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HEO6883280.1"
                     /translation="MAKIQSDASLASSCATSIQTGANGITSVGKATKDSDSQYSGQTS
                     ASLYIDNEASKSGSIAGKLTEFIGLIHSTASEFEAVDQKLSQSISTPKQYTTPSGNST
                     TFQPKPYFN"
     gene            6485..6841
                     /locus_tag="VBC52_001717"
     CDS             6485..6841
                     /locus_tag="VBC52_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883281.1"
                     /translation="MNDKQLKEVERNEAILRKELERIEDKKIVLKKSYDKTINMQLDI
                     QQSLRDSSQSLSPEEVMEQEEIMLIFNRQSRIVEDYFQEEMAKLNKQETDAKDTLEGL
                     VQERQKLYVSQSEKGE"
     gene            6841..8127
                     /locus_tag="VBC52_001718"
     CDS             6841..8127
                     /locus_tag="VBC52_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883282.1"
                     /translation="MVKVNAGSLAAQASSVSTVVASRVTALNSAKQSLTSFANQAGLE
                     GTAYQNAKSYATGVLGTLIDGMILLSEETEEGVSKLQSLYSDKCGGESLDSDVLTKKI
                     EKDEELLAKIHDLQAHLDKHATFLSHAFDGLEATIKARLKKNKKKLKYLMEFNAASST
                     VFDGLTEVQDAVSAGLNYVSEGFGNFNGTFDTSGKGLDWTKTIKTGMEKRRDKVSIDK
                     NSLLKEVKKAYKAGKINKQTYQSIKSGLVNSSTAFLKELAKAKLTDKAVETFTKESWE
                     ALSKNFQTMQFSGVLAAVNGAELSTAIDVNPAYAEMIRGGSQFVKKGVPVIGAAVDFG
                     LQLKDGEDVGDAAVKTGGHVAIGIGAAKAGAIIGSAFPVVGTAGGAVVGFAIGVAGSM
                     AFDWLYDNKEQVYNTTKKVISTRKTIDSVVYEGFWR"
     gene            8133..>8575
                     /locus_tag="VBC52_001719"
     CDS             8133..>8575
                     /locus_tag="VBC52_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883283.1"
                     /translation="MNNKILALGTYYSNDILYDKQTNILYKDVNINTNSNKNYLVVSL
                     TLFSLPIVRWLTQFLHFENSFINSFILFIVIFLTFILTHSLVKQKFNKLQLEVISLTD
                     YEKKEFLIKEKKNTEIAITIFFISLMLSIICMFIYLLISSIILIF"
BASE COUNT         2936 a         1487 c         1605 g         2547 t
ORIGIN      
        1 aaggacaatc tgataggaaa aaacgtatca aacaattatt ttctgaagaa agtaatcaat
       61 ttaaatttgt tacaagaaca gaagaagcga ctgtttcggc tgatattggt catatgatta
      121 tgtacctggt tctcattagt ttggtactaa tattagcttc tataatgact tttaaaataa
      181 aggacaaaaa ggatgaatac acatattaac attactctag atatggggga aacctcaaaa
      241 gatttacgta tccctttgaa aattagcgtt aagcaattag taatggaatt agatagtatt
      301 tttagccgta ctgaaaggcg taccaaatat caactaagag ttataaataa aggtatttta
      361 ttgggagaga acgatatatt agctcaccat ccagtaacaa ctggtgatcg tataaggata
      421 gaggagtttt attaatggaa acagattcgt ttgtatttga agaacaagac tttcaatttg
      481 aaaaaggacc agatttttgg cagttatctc ttaagcgttc aaaagtagga actcaaaatc
      541 tgcaacaact gcaattgtta gaaattcacc attctttgtt gatgcctatg acaactgcaa
      601 ttgatgcaga taccattcaa tttcaattcc agacagaagc gcatgctcta tcttttgaaa
      661 gctttaaaaa agaaacttta tctgaaaaat tacgattagc cttgaatgtc ttggatttag
      721 ataaggcttt gtctttgcca gtaaatttca ttttgcatcc ctctaatcta tttttaacaa
      781 aaaatgcaac tgctaaaata gcttatcgta gccttcctgg gattatgaca cctgagaagt
      841 ttggtccaga agagttttta tatcaattca aatgttttgt ctttgcatta ttgacgcaac
      901 atgactatat agagttgtat aatggtgcta tttctgttat tgaagtatca gatttcctaa
      961 aaagcattta tcatgcagaa actattcagg ctgttagaga catcattact actgattatg
     1021 agcagcaagt agaagtagaa actcatacgt tagcaaaagt atcaaaggca aaatataagc
     1081 tttataaata cataagtgtc tggctaggag ctttatctac aatactcttg attccgctag
     1141 tatatctggt ttttattcac aatccattca aagaaaaaat gttggcagcg gatacttcat
     1201 ttattaaagt tgattataat caagtgatta atcgactaga acatgtaaaa gtaagtaagt
     1261 taccttatac acagaagtac gagttggcct attcctatat taatggaatg tcattttctg
     1321 aagaacagcg tgaagttatt ttaaacaatg ttacgctcaa gactgatgag ctctatcttg
     1381 attattggat taatattggc cgtggtttag atgatgatgc tattgatgct gccaaacgtt
     1441 tagacgattc tgaccttgtt atttatgcta ttgttcagaa aatggatcag gttagaaaag
     1501 acaatagttt atctggtaaa gatcgtgaac aaaaactttc tgagctacaa acagactatg
     1561 ataaatattg gaaagaccgt aagacagctt taacagatga ggaatccaaa tcgaaaaata
     1621 gcaataatca ttcgacaaat tccaacaaag agttatcatc tgaaccatca agtactacaa
     1681 caagtacatc ttctaaaact aaaagtaggt aggtggatat gttagttatt tgttattatc
     1741 aatcactccg ctacgaattt aatattgaag aggagaaatc ctttctaata tcatcaaacg
     1801 gcaagtcgcc aataccagta tcagaattag aaaatgacat cacgctaaaa aatatgcaag
     1861 gtcaattggt atatattatt gaccaaaaag aaaaagagtt aactaatggt gtagagattt
     1921 caggaattgt cttctattta gcgaataatc aaaaagaaat ctatactccc ttagattacg
     1981 aagatatatt aatcggtgat aaagaagggt atcatgtccg ttttaaagaa ggagccccta
     2041 atttattact gaaaaaaata gaaagcaatt ggcagcttaa tttatttgaa ggtgatattt
     2101 atctgaataa tcatcttcaa aaagtagtgc agcagttacc tttaagttta ggtgatgaga
     2161 ttagcttcca aggaacaatt gttaagcttt tcccagatga aattcaaatc tgggggggaa
     2221 ctgattatga aacatcatta actaagaaag ttatgtcagc ttatcaattt tacgcaggct
     2281 atcctgattt tcaccgctca cctcgtatta tttatcgttc cagtgaagat aaaattacag
     2341 ttaatgcgcc tggtaatgaa cctaataagt ctaaagatga actgttaaaa ttaattgttc
     2401 ctcctctagt gatgattgga gtcagcattc ttatttctat ttttcgtcct cgtggtatct
     2461 atatcattgc aacaatgtca atggctctag tgacgatgat tttctctatc actggttact
     2521 ttaaaaatcg taaacagtat aagcaggact tacaagaacg cattgattcc tatcatgact
     2581 atttatctga taagtcaatt gaacttcaga agttagctaa agagcaaaag cgtgggcaac
     2641 actatcatta cccaacgatt gaaggtttac aggaaatggc tgatacttat caccatcgta
     2701 tttatgaaaa gacaccactt cattttgact tcttgtatta tcgcctaggt ttgggagaag
     2761 taccaacgag ctataatatt cattattctc aaccagaacg ttctgggaaa aaagatcctt
     2821 tagagaacga aggctataac ctctacttta acaatcgtta tattaaaaat atgccgattg
     2881 ttgcgaattt atcacatgga ccagttggct atataggacc acgaggttta gtattggagc
     2941 aactccaatt gatggttaat caattagctt ttttccattc ttaccatgat gttcaattta
     3001 ttactattgt tccagaggaa gagatggata agtggtcttg gatgcgttgg ttgccacatg
     3061 caaccttaca agatgtcaat gttcgtggct ttgtttataa tcaaagaagc cgtgatcaag
     3121 tgctcaacag tcttaaccaa attttaaaat tacgtcgcac acaacgcgaa gataaatctg
     3181 ccaaagaagg gactttattt tctccgcatt atgtcgttat tgtaactgat gaaaagttaa
     3241 ttttagatca cgtcatcatg gaattcttta cggaagatcc tacagaattg ggatgttctc
     3301 tcatctttgt tcaagatgtt atgagttcac tttcagaaaa tatcaaaacc attattaata
     3361 ttaaagaccg taatactggt cagttagtta tagaagaagg agagcttaaa gaaacagatt
     3421 ttgaattaga tcatttctta gaggattatg acaaggaaaa tatttcacgt cgcctagctc
     3481 cgttaaatca cctacaaaat cttaaatctt caataccaga agcagttact tttatggaaa
     3541 tgtatcaagc agaagaattt gaagatttgc atgttcaaga gcgttggata agccatgctc
     3601 cgtataaaag cttagctgtt ccacttggct tacgtggtca ggatgatatt gtttatctaa
     3661 acctacatga aaaagctcat ggtcctcatg gattagttgc aggaacaacg ggatcaggga
     3721 agtcagaaat tatacagtcc tatatccttt ctttagctgt taatttccat ccacacgatg
     3781 tagctttcct tctcatagac tacaaaggtg gggggatggc aaatctcttt aaggacctgc
     3841 cacacttact tggcaccatc accaacttag atggtgccca atcgatgcgt gctttggttt
     3901 ctattaatgc tgaattgaaa cgtcgtcaac gtttatttgc taaagctgat gttaatcata
     3961 ttaaccagta tcagaagaaa tacaaacttg gagaagtttc agaaccaatg cctcatctct
     4021 tcttaatctc cgatgagttt gccgaattga aatccaacca acctgagttt atgaaagagc
     4081 ttgtctcaac agcgcgaatt ggtcgttccc tagggattca cttaatccta gcgacccaaa
     4141 aaccatctgg tgtggtagat gaccaaatct ggtcaaactc acgcttcaaa ctagctctta
     4201 aagtagcaga ccgcggcgac tccatggaaa tgctccatac cccagatgcc gccgagatta
     4261 cacaagcagg acgtgcctac ctccaagttg gtaataatga agtctatgaa ctcttccaat
     4321 cagcatggtc aggagcagat taccaacctg aaaaagacga ccaagggatt gaagaccata
     4381 ccatttattc catcaacgac cttggccaat acgaaatcct aaatgatgat ctttcaggct
     4441 tagaccaagc cgaaaatatt aaagaagtac ctacggaatt agatgccatc gttgaaaaca
     4501 ttcaagcttt aaccaaagaa atgggcatct cagacttacc tcaaccgtgg ctaccgcctc
     4561 taagcaatca gatttcagta accgatctcc gtaaggaaga atcagtagac ctctggagca
     4621 aggccccatc ttacaaagct gttcttggct ttatggatat accaagccaa caagcacaag
     4681 aagtagctta ccacgatttt gaagatgatg ggcatttgtc aatttttgca ggaccaagta
     4741 tggggaaatc cacagccctt caaactgtaa caatggactt agcacgtcac aatagtccag
     4801 aatttttaaa cctttatctc tttgattttg gtaccaatgg tctcctacct ctaagacgtt
     4861 tgccacatgt agcagatttc tttaccattg atgatgacga aaaaatagcc aagtttattg
     4921 cccgtatcaa agttgaaatg tctgatcgta aaaaagccct cagtcgctac aatgtcgcga
     4981 cagcaaaact ttaccgtcag gtatctgggg aaaccatgcc tcaaatcctg attgttatcg
     5041 acagttatga agggctacgg gaagctcaga cactgactaa cttagaagct tgtttccaaa
     5101 atatttcgcg tgatgggtcg tcacttggca tttccttagt tatctcagct ggtcgtatgg
     5161 cagctttgag gtcgtcctta atggcaaacc ttaaagaacg tatcgctctt aaattaacag
     5221 atgactctga atcacggaca cttgtcggtc gtcaccaaca catcatggaa gatattccag
     5281 gacgtggatt aatcaaacgt gatgacattg aagtgttaca agtggccctc ccaacagaag
     5341 gcactgaaac ctttgacatc atcagtaaca tccaaaccga atctgacgcc atgaacgaca
     5401 aatggactgg accaagacca aaagcaatac caattgttcc agaagaatta acctttgacg
     5461 actttatggc tacagatagt gttcaagcag atctatcagc taatcgttta ccacttgggc
     5521 ttgaaatggt tgacgttgaa agctttagcc taccattaac gaaatttaag cacctcttat
     5581 tcctatcaga ttcagatgaa ggtttagaaa atctaggcag tcatttactt aaaacgttga
     5641 ttaaagtgcc agaatacagc accatgatca ttgattcctt aggcgaacat gaagcctacc
     5701 aaggacaagt gagaacctat gtcggtgctg atatgattag tgatatggca gaacaactca
     5761 attatgagct aggaaaacgt caagaacaaa acgctttcga tagatggttt atcttaattc
     5821 cagattttga aagtttcgtc agcaaaacaa accttagcct agagcaaatc caaaatctct
     5881 tagataatgg acccaaagtc ggcttacacc tgatcattgg ctcagccttt agctttgttg
     5941 gttcaaaact tgaccccgtt aacaaatacg tcaaaaccaa tagccaatat gttatgctag
     6001 gcatgcgcct aatggaccaa acattcctag agaaagtgta taatagtaaa gaagcacgtc
     6061 tggaacgcga cgaagcctac attcacgata gaaaaaacta tcaaaaacta aaactcagca
     6121 tagactagag aggagagaga atggcaaaaa tacaaagtga tgcatcactg gcatcaagct
     6181 gtgccactag cattcaaact ggagcaaatg gtattacttc agttggtaaa gcaacaaaag
     6241 atagcgacag tcaatattca ggtcagacga gtgcctcatt atacattgat aatgaggcca
     6301 gtaaaagtgg atcaattgca ggtaaattaa cagaatttat tggtctgatt cattctactg
     6361 ctagcgaatt tgaagctgtc gatcaaaaat tgagtcagtc tatttctaca cctaagcaat
     6421 acacgactcc atctggcaat tcaacgacat ttcaaccaaa accgtatttt aactaggagt
     6481 ctttatgaat gataaacagt taaaagaagt cgaaagaaat gaagccattc ttagaaaaga
     6541 actggaacgg attgaagata agaaaatagt cttaaaaaaa tcctatgaca aaactattaa
     6601 catgcaactt gatattcaac agtctctgag agatagttct caaagtcttt caccagaaga
     6661 agttatggaa caagaagaga tcatgttaat ttttaatcga cagtcaagaa tcgttgaaga
     6721 ttattttcaa gaagaaatgg ctaaactcaa caaacaagaa acagatgcta aagacacatt
     6781 agaaggactt gtccaagagc gacagaaact atatgtgtct caatctgaga agggagaatg
     6841 atggtaaaag ttaatgcagg ttcacttgct gcacaggcgt catccgtatc aacagtagtt
     6901 gcttcacgtg ttacagcttt aaatagtgca aagcaaagtc tgactagttt tgctaatcaa
     6961 gcaggcttgg aaggtactgc ctatcaaaat gcaaaatcct atgccacagg tgtattagga
     7021 acattaatag atggtatgat ccttttatct gaagagacag aggaaggtgt tagtaaacta
     7081 caaagtttat atagcgataa atgtggaggt gaaagtctag attctgacgt cctaaccaaa
     7141 aaaatcgaaa aagatgaaga attattagct aaaattcatg atttgcaagc acacttagac
     7201 aaacatgcca cttttttgtc gcatgccttt gatggattag aagcgacaat aaaagcacgg
     7261 ctcaaaaaga ataagaaaaa attaaaatat ctcatggaat tcaatgcggc atcctcaaca
     7321 gtctttgatg gactaacaga agttcaggat gccgtttcgg caggccttaa ctatgtctca
     7381 gaaggttttg gaaatttcaa tggtacattt gatacctcag gtaaaggtct tgattggaca
     7441 aaaacaatca agactgggat ggaaaagagg agagataagg tttcaattga taaaaattca
     7501 ttattgaaag aagtaaaaaa agcttataaa gctggtaaaa ttaataaaca aacttatcaa
     7561 tcaataaaaa gtgggttagt aaatagttcc actgcttttc tgaaagaatt agctaaagca
     7621 aaattaaccg ataaagcagt ggaaacattc actaaggaat catgggaagc attgtccaag
     7681 aatttccaaa cgatgcagtt ctctggtgtt ttagctgctg taaatggagc tgaattatca
     7741 actgcaattg atgtcaatcc tgcttatgca gagatgattc gtggaggtag tcaatttgtg
     7801 aaaaaaggag ttcctgttat tggtgctgca gttgactttg gactacaact taaagatggt
     7861 gaagatgttg gtgatgcagc agtcaagaca ggtggtcatg ttgcaattgg tattggagct
     7921 gcaaaagcag gagctataat agggagtgca tttccggtag tcggaactgc tggtggtgcc
     7981 gttgttggtt ttgctattgg ggttgctggt tcaatggctt tcgattggtt gtatgataat
     8041 aaagaacaag tttataatac tactaagaaa gtaatttcca ctcgtaagac tattgattca
     8101 gtagtttatg aaggtttttg gaggtgaaat taatgaataa taaaatttta gcactaggaa
     8161 cttattactc aaatgatatt ttatatgata agcaaacaaa tattttgtat aaggatgtta
     8221 atataaatac taattcaaat aaaaattatc tagttgtttc actaacttta tttagtctac
     8281 ctatcgtaag atggttgact caatttcttc attttgaaaa tagttttatc aatagtttta
     8341 tcctttttat agtaattttc ttaactttta tacttactca tagtttagta aagcaaaaat
     8401 tcaataaatt acaactagaa gttatctctt taactgatta tgaaaaaaaa gagttcttaa
     8461 taaaggagaa aaaaaatact gaaattgcaa tcacaatttt ctttatttcg ttaatgttaa
     8521 gtattatctg tatgtttata tatttgctaa tttcgtctat tattttgatt tttat
//
LOCUS       DATRNK010000069         8384 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.069, whole genome shotgun sequence.
ACCESSION   DATRNK010000069 DATRNK010000000
VERSION     DATRNK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8384
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.069"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..109)
                     /locus_tag="VBC52_001720"
     CDS             complement(<1..109)
                     /locus_tag="VBC52_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6883284.1"
                     /translation="MKITLHSVAETLLITLYIRAKDAMAKHPILNDQKSL"
     gene            complement(432..908)
                     /locus_tag="VBC52_001721"
     CDS             complement(432..908)
                     /locus_tag="VBC52_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO6883285.1"
                     /translation="MKQDYISYIRSKVGHETIFLTYSGGILTDGKGRVLLQLRADKNS
                     WGIIGGCMELGESSVDTLKREFFEETGLRVEPIRLLNVYTNFQDSYPNGDKAQTVGFI
                     YEVSCPKPVNIEGFHNEETLQLDYFSKEDVKNITIVNEQHQLILDEYFSQTFQMGH"
     gene            1100..1903
                     /locus_tag="VBC52_001722"
     CDS             1100..1903
                     /locus_tag="VBC52_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HEO6883286.1"
                     /translation="MDHFTKLWQDFSKLPNVVAIALGGSRSGDSFDQSSDYDLYVYCA
                     ATPDITSRKRILNKHCHYIELNNHYWELEDNGTLNDGTDIDILYRNIDNFLSDLEDVV
                     EHHNSRIGYTTCFWHNLINCQILYDPEDQLQSLKERFEVSYPSQLQKQIIIQNRNLLT
                     GKLPSYDKQIIKALKRQDFVSTHHRTTAFLDSYFDIIFALNKLTHPGEKRMISYAKKN
                     ATLLPKHFEENIIKLCHLNSNEHTVKETLNDIIMHLDVMLKENFQHFID"
     gene            complement(1995..2981)
                     /locus_tag="VBC52_001723"
     CDS             complement(1995..2981)
                     /locus_tag="VBC52_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEO6883287.1"
                     /translation="MQEYSIEITLQHPDDMMSLFGSNERHLKLIEENLDVIIHARTER
                     VQVLGDSEETLETARLTIEALLVLVNRGMTVNTSDVVTALSMAQNGSIDKFVALYEEE
                     IIKDSYGKPIRVKTLGQKIYVDSVKNHDVVFGIGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKEQTSRLMEREIIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDVSQIDLPKNVKS
                     GLIDAVEKLRNIKKIDFIHLSAKDVVRHPVVAEIINAYSDSESSHKLQPDND"
     gene            complement(3105..4877)
                     /locus_tag="VBC52_001724"
     CDS             complement(3105..4877)
                     /locus_tag="VBC52_001724"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEO6883288.1"
                     /translation="MRYTNGNFEAFARPRKPEGVDKKSAYIVGSGLAGLAAAVFLIRD
                     GQMDGQRIHIFEELPLSGGSLDGVKRPDIGFVTRGGREMENHFECMWDMYRSIPSLEV
                     PDASYLDEFYWLDKDDPNSSNCRLIHKQGNRLESDGDFTLGTHSKELVKLVMETEESL
                     GAKTIEEVFSKEFFESNFWTYWATMFAFEKWHSAIEMRRYAMRFIHHIGGLPDFTSLK
                     FNKYNQYDSMVKPIISYLESHNVDVQFDSKVTNISVDFKNGQKLAKAIHLTVGGEAKI
                     IDLTPDDFVFVTNGSITESTNYGSHDTVAKPNTDLGGSWNLWENLAAQSDEFGHPKVF
                     YKDIPKESWFVSATATIKDPAIEPYIERLTHRDLHDGKVNTGGIVTVTDSNWMMSFAI
                     HRQPHFKEQKENETIVWIYGLYSNVEGNYIKKPIEECTGREITEEWLYHLGVPEMKIH
                     DLSDKQYVSTVPVYMPYITSYFMPRVKGDRPDVIPQGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYTFLNVERGVPEVFNSAFDIRVLLQSLYYLNDKKSVEDMDLPIPAL
                     MRKVGMKKIRGTYLEELLREAHLL"
     gene            complement(5034..5249)
                     /locus_tag="VBC52_001725"
     CDS             complement(5034..5249)
                     /locus_tag="VBC52_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEO6883289.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADYAFDETTFPKHSSDFETV
                     SRYLEDEASFSFNLTDFDDIWEDYLNH"
     gene            complement(5246..5755)
                     /gene="msrA"
                     /locus_tag="VBC52_001726"
     CDS             complement(5246..5755)
                     /gene="msrA"
                     /locus_tag="VBC52_001726"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEO6883290.1"
                     /translation="MERAIFAGGCFWCMVQPFEELDGIESVLSGYTGGHVENPTYKEV
                     CSKTTGHTEAVEIIFNPEKISYADLVELYWAQTDPTDAFGQFEDRGDNYRPVIFYENE
                     EQRQIAQKSKDKLQASGRFDRPIVTSIEPADTFYPAEDYHQAFYRTNPARYALSSARR
                     HAFLEENWH"
     gene            complement(5893..6747)
                     /gene="cvfB"
                     /locus_tag="VBC52_001727"
     CDS             complement(5893..6747)
                     /gene="cvfB"
                     /locus_tag="VBC52_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEO6883291.1"
                     /translation="MNTLLATVITGLVTDENKDFYFIQKDGFTFALSKSEGEHHIGEM
                     VKGFAYTDIQQKARLTTKETFATRDHYGWGTVTEVRKDLGVFLDTGLPDKQVVVSLDV
                     LPELKELWPKKGDRLYVCLDVDKKDRLWALPADPEVFQQMATPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYSEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKKIKQ
                     DQLGTELL"
     gene            complement(6865..7422)
                     /gene="frr"
                     /locus_tag="VBC52_001728"
     CDS             complement(6865..7422)
                     /gene="frr"
                     /locus_tag="VBC52_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEO6883292.1"
                     /translation="MTKEIVTKAQERFEQSHQSLSREFAGIRAGRANASLLDRIQVEY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERAINESDLGINPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKIAIRNIRRDAMDEAKKQEKNKEITEDDLKSLEKDI
                     QKATDDAVKHIDEMTANKEKELLEV"
     gene            complement(7437..8165)
                     /locus_tag="VBC52_001729"
     CDS             complement(7437..8165)
                     /locus_tag="VBC52_001729"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEO6883293.1"
                     /translation="MEPKYQRILIKLSGEALAGDKGVGIDIPTVQSIAKEIAEVHNSG
                     VQIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQYGVDTRVQT
                     AIPMQTVAEPYVRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEAEAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHVEVIKRGLKIMDATASTISMDNDIDLVVFNMN
                     ETGNIKRVVFGEQIGTTVSNKASE"
     gene            complement(8286..>8384)
                     /locus_tag="VBC52_001730"
     CDS             complement(8286..>8384)
                     /locus_tag="VBC52_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6883294.1"
                     /translation="ICNRVFLIKNGYLVEDNEFLKRACSTNCLTNL"
BASE COUNT         2661 a         1685 c         1249 g         2789 t
ORIGIN      
        1 ctaaggattt ttgatcatta agtatcggat gtttagccat cgcatcttta gctctaatat
       61 ataaggtgat taataacgtt tcagcaacgc tgtgtaatgt tatcttcatt catctactct
      121 cctttatgct attttactta gaaatttgga tttctaaaac tggatttctt taagtatatc
      181 attccaatac cgtaagtaaa gcatatcccc accatccgat attccataat tttctttttc
      241 tgtacatctt atgctttcta aaaaaaacag tcactaaatt attgcaagct actgtttttc
      301 tttttaataa ttataaaata tacaaatgag aactccttat tttcttaaca tttatttcta
      361 caagcatgcc taacaacata tcaatagaag ttagaaattc tttaaagtca cacctagtat
      421 ttcccatcct tttaatgccc catttggaat gtttgtgaaa aatattcatc taaaattaat
      481 tggtgttgtt catttacgat cgtaatattt ttgacgtctt ctttagaaaa atagtccaat
      541 tgcaatgttt cctcattatg aaaaccttct atattaactg gttttggaca gcttacttca
      601 taaataaaac cgacggtttg tgctttatcg ccattgggat aagagtcttg aaagttagta
      661 taaacattta acagtctgat tggttccact ctcaatccag tttcttcgaa aaattctctt
      721 ttcaatgtat ctactgatga ctcacctaac tccatacatc caccaataat tccccaagaa
      781 ttcttatcag ctcttaattg gagtaggaca cgtcccttac catctgtcaa aataccacca
      841 gaatatgtta aaaagatagt ctcatgtcca acttttgacc taatgtagga aatataatct
      901 tgtttcattc tatcaccttg tttaaactaa tatgtacgta ttgtagcata aaacatctcc
      961 ttttcaaatc actcatatac acttcttgaa tatgagaact gttacctctt ttattaactt
     1021 actattaatt attgactaaa aagagatagc cctgtagaat ggagacacat caatatttta
     1081 catttttagg agagcattta tggaccattt cacaaaactc tggcaagatt tctctaaact
     1141 tccaaatgtt gttgctatag cactcggagg ctcacgctct ggtgacagtt ttgaccaatc
     1201 ttccgattat gatttgtatg tttattgtgc agcaactcct gatatcacta gtcgtaaacg
     1261 tatccttaac aagcactgtc attacattga acttaacaat cattactggg agcttgaaga
     1321 taatggtact ttaaacgacg gaactgatat tgatattctc tatcgtaaca tagataactt
     1381 tttatcagac ttagaagatg ttgttgaaca ccacaattct cgaattggat acaccacttg
     1441 tttttggcat aacctcatca attgccaaat actctatgat cctgaagatc aattacaatc
     1501 actcaaagag agattcgaag tttcttatcc cagtcagtta caaaaacaaa ttatcattca
     1561 aaatcgtaac ttattaactg gcaagcttcc ctcttacgat aaacaaatta taaaagccct
     1621 taaacgccaa gactttgtta gtactcacca tagaactact gctttcttag attcctactt
     1681 tgatattatc tttgcactta ataagttgac acatcctggc gaaaaaagaa tgatttccta
     1741 tgctaagaag aatgctacat tgcttcctaa acatttcgaa gaaaatatca ttaaactatg
     1801 ccatctcaac tccaacgaac acactgttaa agaaacatta aacgatataa taatgcatct
     1861 cgacgtcatg cttaaagaaa attttcaaca ctttatagat taagttgaag tacaccatca
     1921 taaaaacgcc taaagcatta ggcgtttttt tacatattgg cacactctct aacaacgtct
     1981 cctagtggca aacatcagtc attatcaggt tgtagcttgt gactactttc tgaatctgaa
     2041 taagcattta ttatttcagc aacgacaggg tgtcttacaa catcctttgc tgataaatga
     2101 ataaagtcaa tctttttgat atttcgtaat ttctcaacag catcaatcaa accagattta
     2161 acattctttg gtaagtcaat ttggctaaca tcgccattta caatcatttt agaattgaag
     2221 ccaagtcgtg ttaggaacat tttcatttgc attatagtgg tgttttgcgc ttcatctaat
     2281 atgacaaatg catcatctaa agtacgacca cgcatgtaag ccaatggagc aatttcgatg
     2341 atttcacgct ccattaagcg acttgtttgc tctttaccta aaatttgata tagggcatca
     2401 taaactggtc tcaaataagg atcaactttc tctttgagat ctccaggcaa gaaaccaagg
     2461 ctttcaccag cttcaaccgc tggtctcgtt aagattattc gtttaacttg accacgcttt
     2521 agtgccgtta ctgctagagt gactgctaaa aaagtttttc ccgttccagc aggcccaata
     2581 ccaaaaacaa catcatgatt tttaacgcta tcaacgtata ttttttgccc taaagtctta
     2641 acacggatag gcttaccata gctatcctta ataatttctt cctcataaag ggcaacgaat
     2701 ttatcaattg aaccattttg tgccatggat aaggctgtta caacatctga agtattcaca
     2761 gtcatcccac ggttaacaag cactaataat gcttcaatag ttagccgagc ggtttctaat
     2821 gtttcttcag agtcacctaa tacctgaaca cgttcagttc tagcatgaat gataacatct
     2881 aaattttctt cgattaattt taaatggcgc tcattgctgc caaagagtga catcatatca
     2941 tctggatgtt gtaaagtaat ttcgatagag tattcttgca aattattctt ccttttcgta
     3001 cagtaagtga ctacattata acaaaatttc attttctttg gtgattttag tcgttgtaaa
     3061 actaaaaaat cgagaaaaac aagttttctc gattttattt tacattataa aagatgagct
     3121 tctcgaagta attcttctaa ataagttcct ctaatttttt tcatgcctac tttacgcatt
     3181 aaggcaggta ttggtaaatc catatcttca acagatttct tatcattgag atagtataaa
     3241 gattgcaaca agacacgaat atcaaatgct gaattaaaga cttctggaac accacgttca
     3301 acatttaaga aagtataaac agcttccata gcagttctaa tagaatattc cgttgtaaat
     3361 actgtatctc tggatggaga ttctgcaaaa ttaccaatga aggctaggtt aacagaacct
     3421 tgtgggataa catctggtcg gtcaccttta actcgaggca tgaaataact agtaatgtaa
     3481 ggcatataaa caggaactgt acttacatat tgtttatcag ataaatcatg aatcttcatt
     3541 tcaggaacac ctaagtgata caaccactct tctgtgattt ctcgaccagt acattcttca
     3601 attggttttt taatataatt accttccaca tttgaataga gtccataaat ccaaacaatt
     3661 gtttcatttt ccttttgctc tttgaaatgc ggttggcgat ggatagcaaa actcatcatc
     3721 caattagaat ctgtaactgt aacaatgcct cctgtattta ctttaccatc atggaggtca
     3781 cgatgtgtta aacgctctat ataaggttca atagctggat cttttattgt tgcagtagca
     3841 gaaacaaacc atgactcttt tggaatatct ttgtagaaga ctttaggatg tccaaattca
     3901 tcagattgag cagctaagtt ttcccaaaga ttccaagaac ctcccaaatc ggtattaggt
     3961 ttagctacag tatcatgact accatagttc gtactttcag tgatagaacc attcgttaca
     4021 aaaacaaaat catctggagt aagatcaatt atttttgctt caccaccaac tgtgagatga
     4081 atagcttttg caagcttctg accgttctta aagtctacgg agatattagt caccttgcta
     4141 tcaaattgaa catccacatt gtgagactct aaataactga tgattggttt caccatagaa
     4201 tcatattgat tatatttatt aaattttaat gaagtgaaat caggcagacc accgatatga
     4261 tggatgaagc gcatagcata tcgacgcatt tcaatcgctg aatgccattt ctcaaaggca
     4321 aacatagtag cccaataagt ccaaaaatta ctttcaaaaa attcttttga aaagacttct
     4381 tcaatcgttt tagcacctaa agactcttca gtctccatga ctagcttaac taactctttg
     4441 gaatgtgttc cgagtgtaaa atcaccatca gattctaagc gattcccctg tttatgaatg
     4501 aggcgacagt tagatgaatt gggatcatcc ttgtcaagcc aataaaattc atctagataa
     4561 gaagcatctg gaacttcgag agaggggatg gaacggtaca tatcccacat acattcgaag
     4621 tgattttcca tttcacgacc accacgcgtt acaaaaccga tatcaggtcg tttgacaccg
     4681 tcaagtgatc ctccagaaag aggtagttct tcaaaaatat gaatacgttg accatccatt
     4741 tgaccatcac gtattaaaaa gacagcggca gctaatcctg ctaaaccaga accaacaata
     4801 taagcggatt ttttatccac accttcaggt tttcgaggtc ttgcaaaggc ttcaaaattt
     4861 ccatttgtat atctcataaa caacctccct agggctatat tttagatgac tatattctac
     4921 tcctttgtga aaacgattgc cattaaaaaa cctgcttttc agacagtctt agacaatgtg
     4981 tccaatttct ataacctaaa aaagagattg aaaatacaat ctctatatta ttgttaatga
     5041 ttgagataat cttcccaaat atcatcaaaa tctgttaaat taaaggaaaa actagcctca
     5101 tcttctaaat aacgactaac tgtttcaaaa tccgaactat gttttggaaa agtggtttca
     5161 tcaaaagcgt aatctgctag gattgctact ggttcatttg attttggatt tctttgtgtc
     5221 atcaaccaac tgtaaaatga ttttctcaat gccagttctc ctctagaaat gcatgtcttc
     5281 tggcactact caaggcgtaa cgagcagggt tagtccgata gaatgcttga tgatagtctt
     5341 ctgctgggta aaaagtatca gcaggctcaa tactggttac aattggtcta tcaaatctac
     5401 ccgaagcttg tagcttatct tttgattttt gagcaatctg acgttgctct tcattctcat
     5461 aaaagatgac aggacgataa ttatccccac gatcttcaaa ttgaccaaaa gcgtctgttg
     5521 gatctgtttg tgcccaatat aactcaacta aatctgcata agagattttt tcaggattaa
     5581 aaataatttc aacagcttct gtatgtccag tagttttact acaaacttct ttataagttg
     5641 gattctcaac atgtcctcca gtgtatcctg ataaaacaga ttcaatacca tctaattctt
     5701 caaatggttg taccatacac caaaaacaac ctccagcaaa aatcgcacgt tccatatcgt
     5761 tctccattct ttttaataga tacattataa caaaaaaagc caaaatttcc tctcagcttt
     5821 tttctataat tagccattaa ttctagctat aactaaaatg acatcgtgag aaaactttaa
     5881 aagttcaaaa gtttataata attctgtacc tagctgatct tgtttgatct ttttagcttt
     5941 catgagtcct ccaagtgctt ttttaaactg acctttggaa attccaaaag tagctttaat
     6001 ttcctctgga gaagatttat catttaaagt catgaatcct ccgttacttt ctaaatacgt
     6061 taaaatcatt tgtgcgtcat tttctaacat ttcaaaagaa cgaggcttta atgataaatt
     6121 taaggttcta tcaacttctc taaagccaat aactcgtgca tccagcactt gccctaaacg
     6181 aggttcactg taacgttcac tagggtgaat aaagccgagc atattatttt ctggtagata
     6241 aacaaaagtt cctgacaatt ttaagcgata aacgattgct ggccaatttt gattttgcat
     6301 attgttatat gctggggttg ccatttgttg aaatacttca ggatctgcag ggagagccca
     6361 gagacgatct tttttatcaa catctaagca aacgtagagg cgatcacctt ttttaggcca
     6421 aagttctttc aattcaggaa gaacatccaa agaaacaacc acttgcttat ctggaagacc
     6481 agtatcaaga aatactccca agtctttacg tacctcagta actgtccccc aaccataatg
     6541 atctcgagtc gcaaatgttt ctttagttgt aagacgtgct ttttgttgta tatctgtata
     6601 ggcgaatcct ttaaccattt caccaatgtg atgctctcct tctgactttg ataatgcaaa
     6661 ggtaaagcca tctttttgga taaagtaaaa atctttgttt tcatcagtta ctaagcctgt
     6721 tatgactgtc gccaataatg tattcatttt tctcctttta aaaaggtaac cgacaaacca
     6781 aatccatctg gttttgattc tatcagttac ctccataatc attttgttac ttgccaatca
     6841 taataaagat agcaaacgtt actattaaac ttccaacaat tctttttctt tattagcagt
     6901 catctcatca atatgtttta cagcatcatc agtagccttt tgaatatctt tttcaagaga
     6961 tttcaagtca tcttctgtaa tttctttatt cttttcttgc ttcttagctt catccatagc
     7021 atcacggcga atattacgga tagcaatctt agcattttct ccgacttttt tcacttcttt
     7081 agctaaatca cgacgtgttt cttctgtcaa agctggaata acaagacgaa taactgaacc
     7141 atcatttgca ggattaatac ctaaatctga ttcgttaata gcacgctcaa tatctttgat
     7201 tgatgattta tcaaatggtg atattaaaag aacacgagct tctggtacag taatagaggc
     7261 taattggtta agtggagttg gcgctccata atactcaact tgaatacgat caagcaagct
     7321 agcattagca cgtcctgcac gaatccctgc aaattctctt gataaacttt ggtgtgattg
     7381 ctcaaaacgc tcttgagctt ttgtaacgat ttccttagtc atatttttat acactcctat
     7441 tcagatgctt tgtttgaaac agttgttccg atttgttctc caaaaacaac acgcttaata
     7501 ttaccagtct cattcatatt gaaaacaaca aggtcaatat cattatccat tgaaatagtt
     7561 gatgcggttg catccatgat ttttaaccct cgttttatta cttcaacgtg agttaattca
     7621 tcaaatttga cagcattggc atctttcttt ggatcagcat tataaacacc atctactcca
     7681 tttttagcca ttaaaattgc ttctgcctca atctcagcag cacgaagcgc tgctgttgta
     7741 tcagttgaga aataaggcga accaataccc gctccaaaaa caacaatacg attcttctct
     7801 agatgacgta aagcacgacc acgtacgtaa ggctcagcaa cagtttgcat tggaatagct
     7861 gtttgaactc tagtatctac cccatattgt tgaaggctat cagccattac aagagcattc
     7921 attactgtcc ctaacatccc tgtataatca gcttgaacac gatccatccc tgcttctgct
     7981 gcaggttctc cacgccaaag attacctcca ccaatgacaa gtgctatttg aacacctgaa
     8041 ttgtgtactt cggcaatttc ttttgcaata gattgaacgg taggaatgtc aataccgaca
     8101 cccttatctc ccgccaatgc ttcaccagat aacttaatta atatacgttg atatttaggt
     8161 tccactacta tgttctcctt attttgtctt tattatttta ccataaaagg caaaatttta
     8221 atagagcaat gacactagta agttgatttc tttatcaact cctaaaataa gaaaaacata
     8281 ataattcaga gattggtcaa acagttagta gaacatgctc tcttcaagaa ttcattatct
     8341 tctactaaat aaccattttt tatcagaaag acccggttac agat
//
LOCUS       DATRNK010000070         8339 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.070, whole genome shotgun sequence.
ACCESSION   DATRNK010000070 DATRNK010000000
VERSION     DATRNK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8339
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.070"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..210
                     /locus_tag="VBC52_001731"
     CDS             <1..210
                     /locus_tag="VBC52_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO6883295.1"
                     /translation="LLMSFIISMIIWLMNLVIVPDSRLVSLVYIIVIGAIGLGVYGFM
                     ALATHLLDKMIGSRAQDLRRKLHLN"
     gene            345..1565
                     /gene="pepT"
                     /locus_tag="VBC52_001732"
     CDS             345..1565
                     /gene="pepT"
                     /locus_tag="VBC52_001732"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEO6883296.1"
                     /translation="MSYEKLLERFLTYVKINTRSNPNSTQTPTTQSQVDFALTVLKPE
                     MEAIGLKDVHYLPSNGYLVGTLPATSDRLRHKIGFISHMDTADFNAENITPQIVDYKG
                     GDIELGDSGYILSPKDFPNLNNYHGQTLITTDGKTLLGADDKSGIAEIMTAMEYLASH
                     PEIEHCEIRVGFGPDEEIGIGADKFDVKDFDVDFAYTVDGGPLGELQYETFSAAGLEL
                     TFEGRNVHPGTAKNQMINALQLAMDFHSQLPENERPEQTDDYQGFYHLYDLSGTVDQA
                     KSSYIIRDFEEVDFLKRKQLAQDIADNMNEALQSERVKVKLYDQYYNMKKVIEKDMTP
                     INIAKEVMEELDIKPIIEPIRGGTDGSKISFMGIPTPNLFAGSENMHGRFEFVSLQTM
                     EKAVDVILGIVAKD"
     gene            1594..2085
                     /locus_tag="VBC52_001733"
     CDS             1594..2085
                     /locus_tag="VBC52_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEO6883297.1"
                     /translation="MIKLFGKIRYHWQPELSWAIIYWSIAIAPIFIGLSLLYERTEIP
                     SQVFVLFAIFIVLVGIGFHRYFVIEEDGYLRIVSFNFLRRSKFPIEDIAKIEVTKSSV
                     TIKFNNNHERIFYMRKWPKKYFLDALAIEPKFKGEVELLDNLIKMDYFECYRYDKKAL
                     TKS"
     gene            complement(2072..2269)
                     /locus_tag="VBC52_001734"
     CDS             complement(2072..2269)
                     /locus_tag="VBC52_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEO6883298.1"
                     /translation="MKVALIPEKCIACGLCQTYSNIFDYQDDGIVKFSDTDNLEKEIP
                     NSDQDTVLAVKSCPTKALTIL"
     gene            2315..2839
                     /locus_tag="VBC52_001735"
     CDS             2315..2839
                     /locus_tag="VBC52_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO6883299.1"
                     /translation="MAKKPWEKKVVENNSHRKDKITRTSRGVVSSTPWITAFLSAFFV
                     IVVAILFIVFYTSNRGEDRAKETSGFYGASSQKVNSSKTKKASTSKKTTSSSKTKTSD
                     KSTTSSSTVASSSETSTSSSVNGGTIVVQPGEGAASIAARAGISVARLQELNPGHMTS
                     GYWYANPGDPVVVN"
     gene            2850..3533
                     /locus_tag="VBC52_001736"
     CDS             2850..3533
                     /locus_tag="VBC52_001736"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEO6883300.1"
                     /translation="MNSINIAIDGPASSGKSTVAKIIAKNLNYTYLDTGAMYRCATYL
                     ALQHGYEAQDVSKILGLLAERPISFGKAEDGSQTVFLGTEEVTLAIRQNDVTNNVSWV
                     SAIPEIREELVNQQRRIAKDGAIIMDGRDIGTVVLPDAELKIFLVASVDERAERRFKE
                     NQEKGIESDFETLKSEIAARDYKDSHREVSPLEAAEDAIEFDTTGVSIEGVVTFIQEK
                     AEKIIDMKN"
     misc_feature    3554..3679
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            3694..4224
                     /gene="infC"
                     /locus_tag="VBC52_001737"
     CDS             3694..4224
                     /gene="infC"
                     /locus_tag="VBC52_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEO6883301.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQAIADD
                     ANVDLVLIQPQATPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPIPDKK"
     gene            4264..4464
                     /gene="rpmI"
                     /locus_tag="VBC52_001738"
     CDS             4264..4464
                     /gene="rpmI"
                     /locus_tag="VBC52_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEO6883302.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAFTSHRFHGKTKKQRRHL
                     RKATMVSSGDFKRIKAMLTRLK"
     gene            4522..4881
                     /gene="rplT"
                     /locus_tag="VBC52_001739"
     CDS             4522..4881
                     /gene="rplT"
                     /locus_tag="VBC52_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEO6883303.1"
                     /translation="MARVKGGVVSRKRRKRVLKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AAAFTALADAAKAKLGK"
     gene            complement(5123..7267)
                     /locus_tag="VBC52_001740"
     CDS             complement(5123..7267)
                     /locus_tag="VBC52_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEO6883304.1"
                     /translation="MKKIKDFASRAINTRLGFILLLVVIYWLKTIWAYHTDFNLGLEN
                     SYQLFLTIINPIPLGLLIIGLALYVKRTKAFYITAFITYAIVNILLIANAIYYREFSD
                     FITVSAVLASSKTSAGLGDSALNLLRIWDLVYVFDFIILIFLFATKKIHLDDRPFNKR
                     ASFSITALSGLLFSINLFLAEIDRPELLSRGFSNTYIVKALGLPSFSIYSGNQTYQAQ
                     KERNGATAQELATAKKYVAEHYAKPNPEYYGIGKGRNVIMIHLESFQQFLIDYKLNVD
                     GKEHVVTPFINSLYHSKETISFSNFFHQVKAGKTSDAETLMENSLFGLSSGSFMVNYG
                     GENTQFAAPHILAQNGGYSSAVFHGNVGTFWNRNNAYKQWGYDYFFDSSYFSKQTKDN
                     SFQYGLNDKYMFADSIKYLEHMQQPFYTKFITVSNHYPYTSLKGESDEEGFPLAKTND
                     ETINGYFATANYLDTALKSFFEYLKAAGVYDNSIIVMYGDHYGISNTRNPSLAELLGK
                     DPETWSEYDNAMLQRVPYMIHIPGYSKGFISNTYGGEVDNLPTLLHILGIDTSKYTQL
                     GQDLLSKDNKQMVAMRTTGQYITPKYTNYSGHLYYTDSGQEITNPDETTKAEIKAIRD
                     ATNKQLSTSDSIQTGDLLRFDENNGLKTVEVEKFNYTHSLKALKAKERKLKDRSTSIY
                     SKHNNKSTVDLFHAPSYLELQDPNKTHKTSKK"
     gene            7402..>8339
                     /locus_tag="VBC52_001741"
     CDS             7402..>8339
                     /locus_tag="VBC52_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEO6883305.1"
                     /translation="MNKLKVNSVVERKIKSGAQLLEKKDFDTSLVNQLVQLFSQSNQF
                     LGMAYLSPQNKGIGWLLSRQVFDFNHDYFVSLFEKSREKRQKFEKSSQTTAYRLFNQD
                     GDNFGGLTIDFYSDYALFSWYNEFVYTNRQMIVAAFKQVYPNIKGAYEKIRFKGLDFE
                     SAHLYGQEAPESFLILENNIKYSVFLNDGLMTGIFLDQHDVRKALATNLSEGKKVLNM
                     FSYTAAFSVAAAVGGALETTSVDLAKRSRELSKAHFDANQIVTDNHRFIVMDVFEYYK
                     YAKRKHLSYDVIVIDPPSFARNKKQTFSVTKDYYKL"
BASE COUNT         2784 a         1286 c         1590 g         2679 t
ORIGIN      
        1 ctattaatga gctttattat ttctatgata atatggttaa tgaacctggt tattgtaccc
       61 gatagtagac tggtaagtct agtttacatt atcgttatcg gtgcaatagg tttaggggta
      121 tatggcttta tggcactggc cacccatcta cttgataaga tgattggcag tcgtgctcaa
      181 gacttacgcc gaaagttgca ccttaactaa tgaaaaacta ggttagacag aattgtttaa
      241 cctagttttt tgacgaccaa aattatttgt gaaattttaa ggtagtcaca atatagtgag
      301 cttattttta tgttataatt aaatggagaa atatgaggtg aaagatgtct tacgaaaagc
      361 ttttagaacg atttttaaca tacgtcaaaa taaatacaag aagtaatcct aatagtacac
      421 aaacgccaac aactcaaagt caagttgact ttgctttaac agttttaaaa ccagaaatgg
      481 aagcaattgg tttaaaagat gttcattatt taccttctaa tgggtatttg gttggaacct
      541 tacctgctac aagcgaccgc ttacgccata aaataggttt tatatcccat atggatacag
      601 ctgattttaa tgctgaaaat attactccac aaattgttga ctataaaggt ggagatattg
      661 aacttggaga ctcaggttac attttaagtc caaaagattt tccaaattta aataattacc
      721 atgggcaaac attgattaca acagatggta aaaccttact gggagcagac gataagtctg
      781 gtatagcaga aatcatgaca gctatggaat atttggcttc gcatccagaa attgagcact
      841 gtgaaattag agttggcttt ggaccagatg aagaaattgg tataggtgca gataaatttg
      901 atgttaaaga ttttgatgtt gattttgcct atacagtgga tggtggacca ctaggagaat
      961 tacagtatga aacctttagt gcagctggtt tagagcttac atttgaagga cgaaacgttc
     1021 accctggaac tgcaaaaaat caaatgatta atgctttaca gcttgctatg gattttcata
     1081 gtcaattacc agaaaatgaa cgtcctgaac aaacagatga ctatcaagga ttttatcact
     1141 tatatgattt aagtggaaca gttgatcaag ctaaaagttc atatatcatt cgagattttg
     1201 aggaagttga tttcttaaaa cgtaagcaat tggctcaaga tatcgctgat aatatgaatg
     1261 aagcattaca atctgaacgt gtaaaggtta aactatacga tcaatattac aacatgaaga
     1321 aagttattga aaaagacatg acacctatca acattgctaa agaagtaatg gaagagttag
     1381 acatcaagcc aatcatagaa ccgattcgtg gtggtacaga tggctctaaa atttccttta
     1441 tgggaatccc tactcctaat ctttttgcag gtagtgaaaa catgcatgga cgctttgaat
     1501 ttgttagtct acaaacaatg gaaaaagcag ttgatgttat tttaggcatc gttgctaagg
     1561 attagtaaag aaatatcaga ttggagtggt taaatgataa aattatttgg gaagatacgt
     1621 tatcactggc aacctgaact ttcttgggct attatttatt ggtcaatagc cattgcacca
     1681 atttttattg gtttatctct gctatatgaa agaactgaga ttccgagtca ggtatttgtt
     1741 ctatttgcca tctttattgt tttagtgggc attggatttc atcgctattt tgttattgaa
     1801 gaagatggat atcttaggat tgtatctttt aactttttaa gacgatctaa gtttccaatt
     1861 gaagatattg ctaaaattga ggtcactaaa tcaagtgtca ctatcaaatt taataataac
     1921 catgagcgta ttttttacat gcgaaaatgg cctaaaaaat attttttaga tgcactagct
     1981 attgaaccta aatttaaggg agaagtggag cttctcgata atttgattaa aatggattat
     2041 tttgaatgtt atcgctacga taaaaaagct cttacaaaat cgtaagagct ttggtgggac
     2101 atgatttaac tgctaaaaca gtatcttgat cagaatttgg aatttctttt tctaggttat
     2161 ctgtatcgga aaatttaacg ataccatcat cttgataatc aaaaatattt gaatatgttt
     2221 ggcaaagccc gcaggcaata catttttctg gaattagtgc aactttcata gtattattgt
     2281 aatacttatt gatacgatta acaaggggga agtcatggcg aaaaaaccat gggaaaaaaa
     2341 agttgtggaa aacaatagtc atagaaaaga caaaattaca agaacgagta gaggtgtggt
     2401 gtcaagtact ccttggatta ccgctttctt gagtgcattc tttgttattg tcgttgctat
     2461 tttatttata gtattttata cttcaaatag aggagaagac agagcaaaag aaacttctgg
     2521 tttttatggt gcttcaagtc aaaaagttaa tagttctaaa acgaaaaaag catctacaag
     2581 taagaaaaca acctcatcat caaaaactaa aactagtgat aaatcaacaa catcttctag
     2641 tacagtagca agttcgagtg agacatcgac atcatcttca gtaaacggtg gaacaatcgt
     2701 agttcaacca ggtgagggag cagcctccat agctgctcgt gcaggtatct cagttgctcg
     2761 tttacaagaa cttaatcctg gtcatatgac ctcagggtat tggtatgcta atccaggcga
     2821 tcctgtggtt gtaaattaag gggaggatta tgaactcaat taatattgct attgatggac
     2881 cagcatcaag tggaaaaagt actgtcgcta agattattgc taaaaatttg aattacactt
     2941 accttgatac aggtgctatg tatcgttgtg ctacttatct ggctttacag catggatatg
     3001 aagcacaaga tgtttcgaaa attttaggac tcctagctga aagaccgatt tcttttggta
     3061 aagcagaaga tggcagccag acagtctttc ttggcacaga agaagttact ttagctatcc
     3121 gtcaaaacga tgttactaac aatgtctcgt gggtttctgc tattccagag attcgtgaag
     3181 agttagtaaa ccagcagcgt cgtatagcca aagatggtgc tattataatg gatggtcgtg
     3241 acataggaac agttgttttg ccagatgcag aattaaagat ttttttagtg gcttctgttg
     3301 atgaaagagc tgagcgtcgc tttaaagaaa atcaagaaaa aggcattgag agtgattttg
     3361 aaacactgaa atcagaaatt gctgctcgtg attataaaga tagccatcga gaagtttcac
     3421 cattagaggc agcagaagat gctattgaat ttgatactac aggagtatca attgaaggtg
     3481 ttgtaacttt tatccaagaa aaagcagaaa aaataattga catgaagaat tagtcatgat
     3541 atactagttg caatgagaaa agcagaagtg agaacttctc gccttgcgac ttaggttgtc
     3601 tggcccaaca tgtcaagatt ccatctagga agattatatg tgcgggcttg atttatcaag
     3661 tccgctttcg tttttctcta aaaaaataaa gaggtgaaga tcatagctaa aaaagatcta
     3721 ttcattaacg atgaaattcg cgttcgtgaa gttcgtctgg ttggtctcga gggtgaacaa
     3781 ttaggtatta aaccattgtc tgaagctcaa gcaattgcag atgatgctaa tgttgatttg
     3841 gttttaattc agccacaagc tacaccacct gttgcgaaaa ttatggacta tggtaagttc
     3901 aaatttgagt atcaaaagaa acaaaaagaa caacgtaaaa aacaaagcgt tgttactgtt
     3961 aaggaagttc gtcttagccc agttattgat aaaggggatt ttgaaacaaa acttcgtaac
     4021 ggtcgtaagt tccttgaaaa aggtaacaaa gttaaagttt ctatccgatt caaaggacgt
     4081 atgattactc ataaagaaat tggagcaaag gtattggcgg aatttgctga agcgacacaa
     4141 gatatcgcta ttattgagca aagagctaag atggatggtc gccaaatgtt tatgcaactt
     4201 gcaccaattc cagacaagaa ataattgtcg actttatagt attatttaag aggagaattt
     4261 aaaatgccaa aacaaaaaac acaccgcgca tcagctaaac gctttaaacg cacaggttca
     4321 ggtggattga aacgcttccg cgcgtttact tcacaccgtt tccacggtaa aactaaaaaa
     4381 caacgtcgtc atcttcgtaa agctactatg gtttcatcag gagatttcaa acgtattaaa
     4441 gcaatgctta ctcgccttaa ataataaggt ctctgtaaac atttactaca tattataaag
     4501 gattatttgg aggaaatata aatggctcgt gttaaaggtg gagttgtttc acgtaaacgt
     4561 cgtaaacgcg tacttaaatt agctaaaggt tactatggtg caaaacatat cttgttccgt
     4621 actgcaaaag aacaagtaat gaattcttac tactatgcat accgtgaccg tcgtcagaaa
     4681 aaacgtgact tccgtaaatt atggatcact cgtatcaatg cggctgctcg tatgaatggt
     4741 ttatcatatt cacaattgat gcacggtttg aaattagctg aaatcgaagt taaccgtaaa
     4801 atgcttgctg atttagcagt taacgatgca gcagctttca cagctcttgc agatgcagct
     4861 aaagctaaac ttggtaaata agaattaaaa aagttgggtt tgcctaactt tttttaatta
     4921 ctagaaagaa agtacctata ttataatttt agtgaaaagt ttcgttctat atatgtagaa
     4981 tgaaactttt ttgttttatt tcgatatctt tattattgtt ataattttcc tttttgattt
     5041 ttattgtttt atagtattat tccgtataaa aagcacctca atcgttggat tgaggtgtgg
     5101 attaaacttt aaatggcaag gattattttt tagatgtttt atgtgtctta ttaggatctt
     5161 gcaattccaa gtaacttgga gcatggaaga gatcaactgt tgatttgttg ttatgtttgc
     5221 tgtatatgct tgttgaacga tctttcaatt tacgttcttt agctttgagt gctttaagag
     5281 aatgtgtgta attgaatttt tcaacttcta cagttttcaa accattgttt tcatcaaatc
     5341 gtagtaaatc tccagtttga atcgaatcac ttgtagataa ttgtttatta gtagcatctc
     5401 gaatagcttt tatttcagct ttagttgttt catcaggatt tgtgatttct tgtcctgaat
     5461 cagtgtaata gagatgtcca ctatagttag tgtatttagg agtaatatac tgaccagttg
     5521 ttctcatagc gaccatttgt ttattatctt tagataataa gtcttgtcct aactgagtat
     5581 atttagacgt atctattcct agaatatgaa gtaaagttgg taaattatct acctcaccac
     5641 cgtatgtatt actgataaaa ccttttgaat agccaggaat gtggatcata tatggaacac
     5701 gttgtaacat agcgttgtcg tattcagacc atgtttcagg gtctttacct aataattcag
     5761 ctaagctagg attgcgtgta ttagaaatac cgtagtgatc cccatacatt acaataatag
     5821 aattatcata cacaccagca gcttttagat attcaaaaaa tgatttaaga gctgtatcaa
     5881 ggtagtttgc tgtggcgaaa taaccgttaa tcgtttcatc gttagtcttt gcaagtggga
     5941 atccctcttc gtcagactct cccttcaaac ttgtgtaagg gtaatggtta cttactgtaa
     6001 taaatttagt ataaaacggt tgttgcatgt gctctagata tttgatagag tcagcaaaca
     6061 tatatttatc attcaaaccg tattggaatg aattatcttt ggtttgtttt gagaagtagc
     6121 tagagtcaaa gaagtagtca taaccccact gtttgtaagc attatttcta ttccaaaatg
     6181 ttccgacgtt tccgtggaag accgcactac tgtaaccacc attttgagct aagatatgtg
     6241 gagcagcaaa ttgtgtattt tcaccaccat aattaaccat gaatgaacca ctacttagac
     6301 caaaaagtga gttttccatg agtgtttcag catcggatgt cttaccagct ttgacttgat
     6361 ggaagaagtt agagaaggaa atagtctctt tcgaatgata aaggctgtta ataaatggag
     6421 taaccacatg ttctttacca tcaacattta atttatagtc aatcaagaat tgttgaaaac
     6481 tctccaagtg aatcatgata acgtttcgcc cttttccgat accataatat tctggattgg
     6541 gtttggcata atgctctgct acatattttt tggcagtcgc taattcttgt gcagtggcac
     6601 catttctttc tttctgtgct tgataagttt gattaccact ataaatcgaa aatgatggca
     6661 agccaagagc tttaacaata taggtgtttg aaaaaccacg acttagtagt tctggacgat
     6721 caatttcagc taagaataag ttaattgaaa agagtaaacc agacaaagct gtgatagaaa
     6781 aactagcacg tttattaaat ggacgatcat ctaagtggat tttcttggtt gcaaacaaga
     6841 aaataaggat aatgaaatcg aaaacgtaga caagatccca aattctcaat agattaagag
     6901 cagagtctcc aaggccagcc gaagttttac tactagccaa gacggcgcta actgtaatga
     6961 aatcggaaaa ctctctatag tagattgcgt ttgcaattag aaggatattt acgatagcat
     7021 aggtgataaa agcggtaata taaaaagctt tggttcgctt aacatataag gccaaaccta
     7081 tgattaagag tcctaagggg atggggttta taatggtcaa aaacaactga taggaatttt
     7141 ctaaacctaa attaaaatca gtatggtaag cccaaattgt ctttaaccaa tatataacta
     7201 ctaacaataa aataaagccg agtcgtgtat tgatcgcacg gctagcaaaa tctttaattt
     7261 ttttcacagt aggaacttcc tttaattttt ttcctaaaga taaaatctca gattaaatta
     7321 taacataaaa ttttgtaaca tacggtaaag aaaggtcgaa ttcagtatga aaaaagataa
     7381 aaatttgtta taatactgtt tatgaataaa cttaaagtga attctgtcgt tgaaagaaaa
     7441 atcaaatcag gtgctcagtt actggaaaaa aaagattttg ataccagttt agttaaccag
     7501 ttggttcaac ttttttcaca gtcaaatcaa ttcttaggga tggcctatct ttcaccacaa
     7561 aataaaggta ttggttggtt actatcaaga caagtttttg attttaacca tgattacttt
     7621 gtatcgctat tcgaaaaatc tagagagaag cgtcaaaaat ttgaaaaatc cagtcaaaca
     7681 acagcctacc gcttgtttaa tcaggatgga gataactttg gtggcctaac tatagatttt
     7741 tatagtgact atgctctttt ttcgtggtac aatgaatttg tttatactaa tcgacaaatg
     7801 attgttgcag cctttaagca ggtctatcct aatattaaag gggcatatga aaaaattcgt
     7861 ttcaaaggtt tagactttga aagtgctcat ttgtacggtc aagaggctcc tgaatcattt
     7921 ttgattttag aaaataatat caaatatagt gtctttttga atgatggatt aatgacaggt
     7981 attttccttg accaacatga tgtcagaaaa gccttagcaa ctaatctatc agaaggtaaa
     8041 aaagttctaa atatgttttc atacactgcg gctttttctg tagctgcagc tgttggagga
     8101 gcattagaga caacttctgt tgatttagca aaacgctcgc gtgaactttc aaaagcacac
     8161 tttgatgcta atcagattgt cacagataac catcgattta tcgttatgga tgtttttgaa
     8221 tattataagt atgccaaaag aaaacatcta tcatatgatg tgattgttat tgatccacca
     8281 agttttgctc gaaataaaaa acaaactttt tcggttacta aagattacta taaattaat
//
LOCUS       DATRNK010000071         8328 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.071, whole genome shotgun sequence.
ACCESSION   DATRNK010000071 DATRNK010000000
VERSION     DATRNK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.071"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..671)
                     /locus_tag="VBC52_001742"
     CDS             complement(<1..671)
                     /locus_tag="VBC52_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter (permease)"
                     /protein_id="HEO6883306.1"
                     /translation="MFGKLLKYELKSVGKWYLTLNAAVLLVSIILGLVLKALGGNFST
                     DTNSTSAQIFTIILVLLLAMVISGSLLSTLAIIIKRFYSNIFGRQGYLTLTLPVTTNQ
                     IICSKLLASLLWSIFNIFIVIIGIILVILPLVGIGQFVVAFPEIYKIISSSNAPLFIA
                     YFFLSYVAGTLLIYLSIAVGQLFTNKRVLMGIVSYFGISLLITFLTLIIDSIFHIDLF
                     NSHANA"
     gene            complement(683..1381)
                     /locus_tag="VBC52_001743"
     CDS             complement(683..1381)
                     /locus_tag="VBC52_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6883307.1"
                     /translation="MTQLLQLHHVTKKYHKHTAVNDVTVSIPTGKIIGLLGPNGSGKT
                     TIIKMINGLLQPDKGDIVIDGYRPSVETKKIISYLPDTSYLQENMKIKDVVTLFEDFY
                     NDFDSKVAYQLFEDLNLNPRERLKNLSKGNKEKVQLILVMSRKARLYILDEPIGGVDP
                     AARDYILKTIISNYSNDASVLISTHLISDIEPILDEVIFLKEGEIDLQGNADDLREEH
                     NCSIDALFRERFKK"
     gene            complement(1382..1750)
                     /locus_tag="VBC52_001744"
     CDS             complement(1382..1750)
                     /locus_tag="VBC52_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6883308.1"
                     /translation="MAWEFNEKSPIYSQIAEHIKMQIVSQEIKSGDQLPTVRELAQEA
                     GVNPNTMQRAFTELEREGMVFSQRTSGRFVTEDNLLIGKIRQQVAKAELATFVNNMKK
                     IGYKLDEITVALDHFIKEIN"
     gene            1895..4999
                     /locus_tag="VBC52_001745"
     CDS             1895..4999
                     /locus_tag="VBC52_001745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEO6883309.1"
                     /translation="MFAQLDTRTVYSFMDSLVDLKTYVSKSKSLGYQTIGILDHSNLY
                     AAYHFIQEAQKANLRPIVGFSFDIVVENRSIEVYCIAINTVGYKNLLKLSTAQMSEKM
                     SLNLLTEHLEGVQLILPYQDVLDQLNLPFDYVIGVNLTSPQIPYTKPIIAIDTVRYFQ
                     KNDIETLQMLHAIRDNVLLKDAQYASKNQELKPCQEMTLAFKERFPEALANLESLVEN
                     VAYHFDSDFKLPIFNREIPAGEELRTLTQNNLKSKGLWSDAYQERLEKELTIIHKMGF
                     DDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVSYALNITGIDPVKHNLIFERFLNEE
                     RYSMPDIDIDLPDIYRGEFLRYVRNRYGSMHSAQIVTFSTFGAKQAIRDVFKRFGASE
                     YELTNITKKIHFRDNLTSVYNRNLAFRQIIDSKIEYQKAYDIAKRIEGNPRQTSIHAA
                     GVVMSDDLLTDHIPLKNGEDMMITQYDASSVEDNGLLKMDFLGLRNLTFVQKMKEKVD
                     KDYGISIQLETIDLEDKETLKLFAAGQTKGIFQFEQSGAINLLRRIRPECFEDVVATT
                     SLNRPGASDYTENFINRRFGKEKIDLVDPVIAPILQPTYGIMLYQEQVMQIAQTYAGF
                     TLGKSDLLRRAMSKKNSKEMQKMSQSFLEGAVSKGHRQEDARLIFERMAKFAGYGFNR
                     SHAFAYSALAFQLAYFKAHYSDVFYDIMINYSNSDYLIDAIDFGFVIEKPSINTISYR
                     DRIYKKKIYLGLKNIKGVPNDLAYWISKNQPFQSIEDFLMRLPQQFQKSGFISPLIAI
                     GAFDEFDNNRRKITSNLDSLFTFVNELGSLFADTSYHWLEVEDFSNSEKYEMEQDILG
                     VGISPHPLVGISQKASRPFIPISQVQENSEATILVQLKQVKVIRTKSSGQQMAFLTVM
                     DINSKMDITVFPETFNIVRDDLQEGKYYYLHGKIQKRDERLQMVLNGVQEATEERFWI
                     LLKNHDNDKKISEILSKYKGHIPVYLHYETTKETIQSKVHLVRKDSGLALDLSEFVVK
                     TVYQ"
     gene            5080..6102
                     /gene="pfkA"
                     /locus_tag="VBC52_001746"
     CDS             5080..6102
                     /gene="pfkA"
                     /locus_tag="VBC52_001746"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEO6883310.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINQGYYGM
                     VTGDIFPLDANSVGDTINRGGTFLRSARYPEFAELEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGLPGTIDNDIVGTDYTIGFDTAVATAVENLDRLRDTSASH
                     NRTFVVEVMGRNAGDIALWSGIAAGADQIIVPEEEFNIDEVVSNVRAGYAAGKHHQII
                     VLAEGVMSGDEFAKTMKAAGDDSDLRVTNLGHLLRGGSPTARDRVLASRMGAYAVQLL
                     KEGRGGLAVGVHNEEMVESPILGLAEEGALFSLTDEGKIVVNNPHKADLRLAALNRDL
                     ANQSSK"
     gene            6151..7653
                     /gene="pyk"
                     /locus_tag="VBC52_001747"
     CDS             6151..7653
                     /gene="pyk"
                     /locus_tag="VBC52_001747"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEO6883311.1"
                     /translation="MNKRVKIVATLGPAVEFRGGKKFGESGYWGESLDVEASAEKIAQ
                     LIKEGANVFRFNFSHGDHAEQGARMATVRKAEEIAGQKVGFLLDTKGPEIRTELFEDG
                     ADFHSYTTGTKLRVATKQGIKSTPEVIALNVAGGLDIFDDVEVGKQILVDDGKLGLTV
                     FAKDKDTREFEVVVENDGLIGKQKGVNIPYTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVNEVRAICEETGNGHVKLFAKIENQQGIDNIDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIITKVNAAGKAVITATNMLETMTDKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDKNAQTLLNEYGRLDSSAFPRNNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRSLMINWGVIPVLAD
                     KPASTDDMFEVAERVALEAGFVESGDNIVIVAGVPVGTGGTNTMRVRTVK"
     gene            7824..>8328
                     /gene="lepB"
                     /locus_tag="VBC52_001748"
     CDS             7824..>8328
                     /gene="lepB"
                     /locus_tag="VBC52_001748"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO6883312.1"
                     /translation="MVKRDFIRNIILALIAVLILILLRYFVFATFKVHKDATNSYFSN
                     GDVVVVNRNRTPKYKDFIVYKVGKIFYISRVIGEPNQKVRVMDDILYLNDVFKDEPYI
                     EKMKNAYSEKKDGQMPFTSDFSVETLTRNKESRVPKGSYLVLNDNRQNKNDSRKFGLI
                     KEKDIRGV"
BASE COUNT         2700 a         1298 c         1640 g         2690 t
ORIGIN      
        1 gcatttgcat gagagttgaa taaatcaata tgaaaaatac tatctattat taatgttaag
       61 aaagtaatta agagtgatat tccaaaataa gaaacaattc ccatcaatac acgtttgttt
      121 gtaaataatt gccctacagc gatagagaga taaatcagta atgtccctgc aacgtacgat
      181 agaaagaaat aggcgataaa aagtggagcg ttcgaagatg aaataatttt atagatttct
      241 ggaaaagcga caacgaactg accaatacct actaaaggta atatgaccaa tatgattcca
      301 ataatgacaa taaaaatatt aaaaatactc caaagaagag aagctagtag ctttgaacaa
      361 ataatctgat tcgtagttac tgggagcgtt aaggttagat atccttgacg gccgaagata
      421 ttgctataaa aacgtttgat aataatagca agggtggata acagagatcc tgaaataacc
      481 atcgctaaca ataaaacgag tattatagtg aatatttggg cgctagtact attagtatca
      541 gtagaaaaat tgccgccaag tgcctttaat accagtccta aaataataga tactaataaa
      601 accgcagcgt tgagtgttag ataccactta ccaactgatt ttaattcata ttttaataat
      661 tttccaaaca tatcaatctc ctttatttct tgaatctttc acgaaagaga gcatcaatcg
      721 aacagttgtg ctcttctctt aagtcatcag cattgccttg taggtcaatt tctccttctt
      781 taaggaagat aacttcatca aggataggtt caatgtcaga aattaagtga gtcgaaatta
      841 aaacagaagc atcattagag tagttgctaa tgattgtttt taaaatgtag tcacgcgctg
      901 cagggtctac accaccaata ggttcatcaa ggatgtagag cctagcttta cgactcataa
      961 caagtattaa ttgaactttt tccttgttcc cttttgatag gttttttaga cgttccctag
     1021 gatttaggtt aagatcctca aatagttgat atgctacctt tgaatcaaaa tcgttataga
     1081 aatcttcaaa taatgtaacg acgtctttga ttttcatatt ttcttgtaaa tatgaagtat
     1141 cagggagata tgaaataatt tttttggttt caacagacgg tctgtaacca tcaataacga
     1201 tatctccctt atctggctga agtaatccat ttatcatttt gatgattgtt gtttttccgc
     1261 taccatttgg acctaataaa cctataatct ttcctgttgg aatacttaca gtcacatcat
     1321 taacagcagt atgtttgtga tatttctttg taacatgatg aagttgtaat aactgtgtca
     1381 tttaattaat ctcctttata aaatgatcga gagcaaccgt aatttcatct aatttatatc
     1441 ctattttttt catgttgttg acaaaagttg ctaactctgc ttttgcaact tgttgacgaa
     1501 ttttaccaat tagtaagttg tcttctgtaa caaagcgtcc ggaagtacgt tgtgagaata
     1561 ccattccttc acgttccaat tcagtgaagg cacgttgcat ggtatttggg ttaacaccag
     1621 cttcttgagc taactctctc acggtaggca attggtcacc actttttatt tcttgactaa
     1681 caatttgcat ctttatatgt tcagcaattt gtgaataaat aggtgatttt tcattgaatt
     1741 cccaagccat agataactcc tttcataatc ttgtactttt tgtactataa aaatagtaca
     1801 aaaaaatatt tgaaatgtca aggaattatc attgagactt taatcgttta ttgtttcata
     1861 gtataatagt aaaaaatcgt aaaaaggggc tactatgttt gcacaacttg atacaagaac
     1921 cgtttattct tttatggata gcttggttga tttaaagaca tatgtctcta aatcgaaatc
     1981 attaggatat caaactattg gtatcttgga ccacagtaat ctttatgcag catatcattt
     2041 tattcaagag gcgcaaaagg caaacctaag accaatagtt ggatttagct ttgatatagt
     2101 ggttgaaaat agatcaatag aagtttactg tattgcgata aatacagttg gttataagaa
     2161 tctcttgaag ttgtcaacgg ctcaaatgtc tgagaagatg tctctaaatt tattgacaga
     2221 acatcttgaa ggggtgcaat tgattcttcc ctatcaagat gttcttgatc agctcaattt
     2281 accctttgat tatgttattg gtgttaattt gacctctccg cagattccat ataccaaacc
     2341 cattatagcc atagatactg ttcgatattt tcaaaaaaat gatattgaga cattacagat
     2401 gcttcatgct ataagagata atgtcctgtt aaaagacgca caatatgcga gcaaaaatca
     2461 agaattaaaa ccttgtcaag aaatgactct agcattcaag gaacgttttc ctgaagcttt
     2521 agctaaccta gaatctttgg tagagaatgt tgcttatcac tttgattctg actttaagtt
     2581 acctattttt aatagagaaa ttcctgctgg agaagaatta agaaccttaa ctcaaaataa
     2641 cttaaaaagt aaaggtttat ggagcgatgc ttatcaagag cgtctcgaaa aagaattaac
     2701 aatcattcac aaaatgggct ttgatgacta tttccttatt gtgtgggatt tattaagatt
     2761 tggtcgtagt aagggctact atatgggaat gggacgtggt tcagcagcgg gaagtttagt
     2821 ctcatatgct ttaaatatta ccggtatcga tcctgtcaaa cataatttaa tttttgaacg
     2881 ttttttaaat gaagagcgtt attcgatgcc agatattgat attgatcttc cggatattta
     2941 tcgtggagaa ttcttgcgct atgtacgtaa tcgatatggt tcaatgcatt ctgcacagat
     3001 tgtaaccttc tcaacttttg gtgcgaaaca ggctattcga gatgttttta aacgctttgg
     3061 tgcatcagag tatgagttaa caaacataac taaaaaaatt catttcagag ataatttgac
     3121 cagtgtttat aaccgcaact tagctttcag acaaataata gatagtaaaa ttgaatacca
     3181 aaaagcctat gatattgcta agcgtattga aggaaatcct agacagactt ctattcatgc
     3241 cgctggggta gtaatgagtg acgatttgtt aacagatcat attcctttaa aaaacggtga
     3301 ggatatgatg ataacgcaat atgatgctag ttctgttgag gataatggtc ttctaaaaat
     3361 ggactttcta ggtcttcgaa atttgacatt tgttcagaaa atgaaggaga aagtcgataa
     3421 ggactacggt atctctatac aattagaaac tattgattta gaagataaag agactctaaa
     3481 actatttgca gctggtcaaa caaaggggat ttttcaattt gagcaaagtg gagctattaa
     3541 tttgttgcga cgtattaggc cagagtgttt tgaagatgtg gtagctacca ccagcttgaa
     3601 taggcctggt gcaagtgatt atactgagaa ttttataaac cgtcgttttg gtaaagaaaa
     3661 aatcgattta gtagatcctg tcatagctcc aattttacaa cctacctatg gcataatgct
     3721 ctatcaagaa caggttatgc agattgccca gacctacgca ggttttacgc taggcaagtc
     3781 tgatttgctc agacgtgcta tgtctaagaa aaattctaag gagatgcaaa agatgtctca
     3841 atccttctta gagggtgcag tatctaaggg acatcgtcaa gaagatgctc gacttatatt
     3901 tgaacgcatg gctaaatttg caggttatgg gtttaatcgc agccatgcgt ttgcttattc
     3961 ggctttagct tttcaattag cctatttcaa agcccattat tccgatgtct tttatgacat
     4021 catgataaat tattcaaata gtgattatct aatcgatgct attgattttg gttttgttat
     4081 tgaaaaacct tcaattaata ctatttcata cagagaccgc atctataaaa agaaaattta
     4141 tttaggttta aaaaatatta agggtgttcc taacgatttg gcttattgga tttctaaaaa
     4201 tcaaccgttt cagagtattg aagattttct aatgaggctg ccacagcagt ttcaaaaaag
     4261 tggttttatt tctccgctaa ttgctattgg agcatttgac gaatttgata ataatagacg
     4321 gaaaatcacc tctaatcttg attctttatt tacatttgta aatgaactag gtagtttatt
     4381 tgcagatacc tcatatcatt ggctagaagt agaagatttc tctaattctg agaaatatga
     4441 aatggaacaa gatatcttag gagtaggaat cagcccgcac cctttagtgg ggatttcaca
     4501 aaaagctagc cgtccgttta taccaattag tcaagttcaa gaaaatagcg aagctactat
     4561 tttagttcag ctaaagcagg tcaaagttat taggacaaaa agttctgggc aacaaatggc
     4621 tttcttgact gttatggata ttaattcaaa aatggatatt actgtttttc ctgaaacttt
     4681 caatattgta agagatgatt tacaagaagg gaaatattat tacttgcatg gaaaaattca
     4741 gaagcgagat gagcgattac agatggtttt aaacggtgtt caagaagcaa cggaggaacg
     4801 tttttggata cttctaaaaa atcatgataa cgacaaaaaa atatcggaaa tattgtccaa
     4861 atacaaaggg catattcctg tctatttgca ttatgaaaca actaaagaaa ctatccaaag
     4921 caaagtacat ttggttagaa aagatagtgg cttagccctt gatttatctg aatttgttgt
     4981 gaaaacggtt tatcaataaa attgcatata aagtgcagaa atttacaaat taatatgcta
     5041 taattaaatc gttaaattaa taaaaggagt accaattaaa tgaaacgtat tgctgtttta
     5101 actagtggtg gtgacgcccc tggtatgaac gctgctatcc gtgcagttgt tcgtaaagca
     5161 atttctgaag gtatggaagt ttacggcatc aaccaaggtt actatggtat ggtgacaggg
     5221 gatattttcc ctttggatgc taattctgtt ggggatacta tcaaccgtgg aggaacgttt
     5281 ttacgttcag cacgttatcc tgaatttgct gaacttgaag gtcagcttaa agggattgaa
     5341 cagcttaaaa aacacggtat tgaaggtgta gtagttatcg gtggtgatgg ttcttatcat
     5401 ggtgctatgc gtctaactga gcacggtttc ccagctgttg gtttgccggg tacaattgat
     5461 aacgatatcg ttggcactga ctatactatt ggttttgaca cagcagttgc gacagcagtt
     5521 gagaatcttg accgtcttcg tgatacatca gcaagtcata accgtacttt tgttgttgag
     5581 gttatgggaa gaaatgcagg agatatcgct ctttggtcag gtatcgctgc aggtgcagat
     5641 caaattattg ttcctgaaga agagttcaat attgatgaag ttgtctcaaa tgttagagct
     5701 ggctatgcag ctggtaaaca tcaccaaatc atcgtccttg cagaaggtgt tatgagtggt
     5761 gatgagtttg caaaaacaat gaaagcagca ggagacgata gcgatcttcg tgtgacgaat
     5821 ttaggacatc tgctccgtgg tggtagtccg acggctcgtg atcgtgtctt agcatctcgt
     5881 atgggagcgt acgctgttca attgttgaaa gaaggtcgtg gtggtttagc cgttggtgtc
     5941 cacaacgaag aaatggttga aagtccaatt ttaggtttag cagaagaagg tgctttgttc
     6001 agcttgactg atgaaggaaa aatcgttgtt aataatccgc ataaagcgga ccttcgcttg
     6061 gcagcactta atcgtgacct tgccaaccaa agtagtaaat aagaatttgt caatttgaca
     6121 ctttaaataa aaaggagctc ataaattatt atgaataaac gcgtaaaaat cgttgcaaca
     6181 cttggtcctg cggttgaatt ccgtggtggt aagaagtttg gtgagtctgg atactggggt
     6241 gaaagccttg acgtagaagc ttcagcagaa aaaattgctc aattgattaa agaaggtgct
     6301 aacgttttcc gtttcaactt ctcacatgga gatcatgctg agcaaggagc tcgtatggct
     6361 actgttcgta aagcagaaga gattgcagga caaaaagttg gcttcctcct tgatactaaa
     6421 ggacctgaaa ttcgtacaga actttttgaa gatggtgcag atttccattc atatacaaca
     6481 ggtacaaaat tacgtgttgc tactaagcaa ggtatcaaat caactccaga agtgattgca
     6541 ttgaatgttg ctggtggact tgacatcttt gatgacgttg aagttggtaa gcaaatcctt
     6601 gttgatgatg gtaaactagg tcttactgtg tttgcaaaag ataaagacac tcgtgaattt
     6661 gaagtagttg ttgagaatga tggccttatt ggtaaacaaa aaggtgtaaa catcccttat
     6721 actaaaattc ctttcccagc acttgcagaa cgcgataatg ctgatatccg ttttggactt
     6781 gagcaaggac ttaactttat tgctatctca tttgtacgta ctgctaaaga tgttaatgaa
     6841 gttcgtgcta tttgtgaaga aactggcaat ggacacgtta agttgtttgc taaaattgaa
     6901 aatcaacaag gtatcgataa tattgatgag attatcgaag cagcagatgg tattatgatt
     6961 gctcgtggtg atatgggtat cgaagttcca tttgaaatgg ttccagttta ccaaaaaatg
     7021 atcattacta aagttaatgc agctggtaaa gcagttatta cagcaacaaa tatgcttgaa
     7081 acaatgactg ataaaccacg tgcgactcgt tcagaagtat ctgatgtctt caatgctgtt
     7141 attgatggta ctgatgctac aatgctttca ggtgagtcag ctaatggtaa atacccagtt
     7201 gagtcagttc gtacaatggc tactattgat aaaaatgctc aaacattact caatgagtat
     7261 ggtcgcttag actcatctgc attcccacgt aataacaaaa ctgatgttat tgcatctgcg
     7321 gttaaagatg caacacactc aatggatatc aaacttgttg taacaattac tgaaacaggt
     7381 aatacagctc gtgccatttc taaattccgt ccagatgcag acattttggc tgttacattt
     7441 gatgaaaaag tacaacgttc attgatgatt aactggggtg ttatccctgt ccttgcagac
     7501 aaaccagcat ctacagatga tatgtttgag gttgcagaac gtgtagcact tgaagcagga
     7561 tttgttgaat caggcgataa tatcgttatc gttgcaggtg ttcctgtagg tacaggtgga
     7621 actaacacaa tgcgtgttcg tactgttaaa taatttttga attcaaacct attatttaag
     7681 aataccttaa ataatgggtt ttttatatgt tttgcttttt attattcgat tattgtgact
     7741 agaataggat atgatattaa taattaccct ccttttttta tctatgaaaa catgatataa
     7801 tggaaaaaag attaggagta gttatggtta aacgcgattt tattagaaat attattttag
     7861 cattgattgc tgtattgatt ttgattctct tgagatattt tgtttttgca acctttaaag
     7921 tacataagga tgctacaaat tcttatttta gcaacggaga tgttgttgtt gttaatcgta
     7981 ataggacgcc taagtacaaa gactttattg tctataaggt tggaaagatt ttctatatta
     8041 gtcgtgttat tggtgagcca aatcaaaaag tgagagtaat ggatgatatt ttatatttga
     8101 atgatgtttt caaggatgag ccttacattg aaaaaatgaa aaatgcttat tctgaaaaga
     8161 aagacggtca aatgcccttt acttccgact tctcagttga aacattaaca cgaaataaag
     8221 aaagcagagt tcctaagggt tcttatcttg tgttaaatga taatcgacaa aataaaaatg
     8281 atagtcgaaa atttggatta atcaaagaga aagatattcg tggtgtta
//
LOCUS       DATRNK010000072         7637 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.072, whole genome shotgun sequence.
ACCESSION   DATRNK010000072 DATRNK010000000
VERSION     DATRNK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.072"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..1088
                     /locus_tag="VBC52_001749"
     CDS             <1..1088
                     /locus_tag="VBC52_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6883313.1"
                     /translation="FLAQTTKSSIYKNEWLYTVSNTLYDLSHLLIYFMASYFVASLVW
                     RGLSNQTKESFSASLLGFATVWLLLTSVVPGTDYHYPSQPIWLMLALISYLFIFLTRL
                     LQNRHHIFQYSMLAILLLISSILSHILYRYPNIDLEYLIQNHFSQWLGDGPHQLIPIM
                     LWSILAIFLTLSGLFVPDILFRPYSYFSVVSENLNAALSQHTDKIPYLYTFYTVKNSF
                     AMFGGIGILLSLFLAVLYESRKLQSKNYYKLTLLTLTPLIFDQNLPFLVGLPVILQPI
                     LFIPMVLTTIFAEAFGALMLYLKFVDPAVYTVPSGTPSLLFGFLASNGDWRYLPVTAI
                     ILVVGFFIYRPFVKIAFAKEEQYEKIT"
     gene            1072..1914
                     /locus_tag="VBC52_001750"
     CDS             1072..1914
                     /locus_tag="VBC52_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6883314.1"
                     /translation="MKKLLRWLPPVLVIIILIGMTILGKSYINKVTAHKIKLYNSRMT
                     PTILIPGSSATQERFNSMLAQLNQMGEKHSVLKLTVKKDNSIIYNGQISGNDHKPYIV
                     IGFENNEDGYSNIKKQTKWLQIAMNDLQKKYKFKRFNAIGHSNGGLSWTIFLEDYYDS
                     DEFDMKSLLTMGTPFNFEESNTSNHTQMLKDLISNKGNIPSSLMVYNLAGTNSYDGDK
                     IVPFASVETGKYIFQETAKHYTQLTVTGNNATHSDLPDNPEVIQYVAEKILKNEKGKL
                     PKPH"
     gene            1991..2818
                     /locus_tag="VBC52_001751"
     CDS             1991..2818
                     /locus_tag="VBC52_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEO6883315.1"
                     /translation="MKLISWNIDSLNAALTSESTRALMSRQVIDTLVAEDADIIAIQE
                     TKLSAKGPTKKHLEVLETYFPEYDLVWRSSVEPARKGYAGTMFLYRKGLNPIVSFPEI
                     DAPTTMDNEGRIITLELENCYITQVYTPNAGDGLKRLADRQIWDIKYAEYLATLDSQK
                     PVLATGDYNVAHKEIDLANPSSNRRSAGFTDEERQGFTNLLAKGFTDTFRYLHGDVPN
                     VYSWWAQRSRTSKINNTGWRIDYWLTSNRVADKITKSEMIHSGDRQDHTPIILEIEL"
     gene            complement(2859..3215)
                     /locus_tag="VBC52_001752"
     CDS             complement(2859..3215)
                     /locus_tag="VBC52_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEO6883316.1"
                     /translation="MYTFYEYPKCTTCRSAKKELTELGLTFEAIDIKSNPPKASLLKE
                     LLENSPYDLKKFFNTSGNSYRELGLKDKFDDLTLDQALDLLASDGMLIKRPLLVKDNK
                     ILQIGYRTKYKDLNLV"
     gene            complement(3217..3693)
                     /locus_tag="VBC52_001753"
     CDS             complement(3217..3693)
                     /locus_tag="VBC52_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEO6883317.1"
                     /translation="MLYQEFYQSPLGEIRLLADNLGLSGLYFVGQKYDMLAVNQEEIV
                     NMSNSYTLLGKKWLDAYFSQQNLPSIPLSLRGTAFQTRVWQELQKIPFGDTKTYGELA
                     KELNCQSAQAVGGAIGKNPISLIIPCHRVLGRYGQLTGYAGGLERKSWLLEYEKEK"
     gene            complement(3750..4931)
                     /locus_tag="VBC52_001754"
     CDS             complement(3750..4931)
                     /locus_tag="VBC52_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEO6883318.1"
                     /translation="MVFSVKTFNNINQIGLQELGNRFQIDGDMSENPDAYIIRSQNLH
                     NQDFPSNLKAIARAGAGTNNIPIEEASAQGIVVFNTPGANANAVKEAVIAALLLSARD
                     YLGANRWVNTLTGTDIPKQIEAGKKAFAGNEIAGKKLGVIGLGAIGARIANDARRLGM
                     TVLGYDPYVSIETAWNISSHVQRVKEIKDIFETCDYITIHVPLTNETKHTFDAEAFSI
                     MKKGTTIINFARAELVNNQELFEAIETGVVKRYITDFGDKELLNQKGITVFPHVGGST
                     DEAELNCAIMASQTIRCFMETGEITNSVNFPNVHQIQTAPFRITLINKNVPNIVAKIS
                     TAVSELGINIDNIINRSKGDYAYTLIDLDETDNNKISTLIEEFEGDENIVRVRLIAKK
                     Q"
     gene            complement(4994..5542)
                     /locus_tag="VBC52_001755"
     CDS             complement(4994..5542)
                     /locus_tag="VBC52_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO6883319.1"
                     /translation="MQIRLAFPNEIDQIMLLIEEARAEIAKTGSDQWQKEDGYPNRND
                     IIDDILNGYAWVGIEDGMLATYAAVIDGHEEVYDAIYEGKWLHDNHRYLTFHRIAISN
                     QFRGRGLAQTFLQGLIEGHKGPDFRCDTHEKNVTMQHILNKLGYQYCGKVPLDGVRLA
                     YQKIKEKGETSIYREIDERNPM"
     gene            complement(5610..6701)
                     /gene="serC"
                     /locus_tag="VBC52_001756"
     CDS             complement(5610..6701)
                     /gene="serC"
                     /locus_tag="VBC52_001756"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEO6883320.1"
                     /translation="MTIYNFSAGPAVLPKPVLVKAQSELLNYQGSSMSVLEVSHRSKE
                     FDDIIKGAERYLRDLMGIPDNYKVIFLQGGASLQFSMIPLNIARGRKAYYHVAGSWGK
                     KAYTEAVKLSKTIPFEPILLASSEESVYDYIPEFDEKEIDPKAAYVHVTTNNTIEGTS
                     LYDIPKTNGVPVIADMSSNILAVKYKVEDFAMIYAGAQKNIGPAGVTVVIIREDMINE
                     EPTLSSMLDYKIQSDAGSLYNTPPAYSIYIAKLVFEWVKSLGGVDAMEKANREKSGLL
                     YDYIDSSEFYSNPVRDKKSRSLCNIPFITINKDLDEKFVKEATERGFKNIKGHRSVGG
                     MRASLYNAFPKQGVIELIDFMKTFEAENA"
     gene            6834..7469
                     /locus_tag="VBC52_001757"
     CDS             6834..7469
                     /locus_tag="VBC52_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEO6883321.1"
                     /translation="MILREFCAENLTDLTRLDKAIISRVELCDNLAVGGTTPSYGVIK
                     EANQYLHEKGISVAVMIRPRGGNFVYNDLELRIMEEDILRAVELESDALVLGILTSNN
                     HIDTEAIEQLLPATQGLPLVFHMAFDVIPKSDQKKAIDQLVALGFTRILLHGSSNGEP
                     IIENIKHIKALVEYANNRIEIMVGGGVTAENYQYICQETGVRQAHGTRITQ"
BASE COUNT         2427 a         1442 c         1205 g         2563 t
ORIGIN      
        1 cttttttagc tcaaacaaca aagtccagta tttataaaaa cgagtggtta tatacggttt
       61 ccaatactct ttatgattta tctcatttac taatctactt tatggctagt tattttgtgg
      121 ctagtttagt atggagaggc ttatctaacc aaactaaaga aagtttttct gccagtttac
      181 ttggctttgc cactgtttgg ttgcttctaa cttcagttgt tccaggtaca gattatcact
      241 atcctagcca acccatttgg cttatgctgg ctttgatttc gtatcttttt atctttttaa
      301 ctcgactact tcaaaatcgc catcatattt tccagtatag tatgttagct atactactat
      361 taatctcttc tattttgtca catatcttat atcgttatcc taatattgat ttagaatacc
      421 ttatacaaaa tcacttttct caatggctgg gtgatgggcc acatcaatta attcctatta
      481 tgctttggtc tatcttagct atttttctaa ccttatcagg tctttttgta ccagatattt
      541 tatttcgtcc gtactcctat ttttcagtag ttagcgaaaa tttaaatgct gccttgagcc
      601 aacatacaga taaaatccct tatctgtata ccttttatac tgttaaaaat agctttgcta
      661 tgtttggcgg tattggaatc ctattatcct tatttttagc agttttatat gaaagtagaa
      721 aactacaatc gaagaattac tataaattga cattactgac cttaactcct cttatttttg
      781 atcaaaattt accattttta gtaggtctac ctgtcatttt acaaccaata ttattcattc
      841 cgatggtatt aacaactatc tttgcagagg cttttggagc tcttatgctc tacctaaaat
      901 ttgtcgaccc tgcagtttat actgtcccaa gtggtacacc tagtctacta tttggctttc
      961 ttgcttcaaa tggggattgg cgttaccttc cagtcactgc aattattcta gttgttggct
     1021 tctttattta tcgtccgttt gtcaaaatcg ctttcgctaa ggaggagcaa tatgaaaaaa
     1081 ttacttagat ggcttcctcc tgtacttgtc attattatcc ttataggaat gactatctta
     1141 ggtaagtcct atatcaataa agtaacagct cacaaaataa aactctataa ctctcgaatg
     1201 actcctacta ttttaattcc aggatccagt gctactcaag aacgatttaa cagcatgtta
     1261 gcacagctca accaaatggg agaaaaacat agcgttttaa agttaactgt caaaaaagac
     1321 aatagcatta tctacaatgg acaaattagc ggcaatgacc acaaacccta cattgtcatt
     1381 ggatttgaaa ataatgaaga tggttatagt aacatcaaaa aacaaacaaa atggctacag
     1441 attgctatga atgatcttca gaagaaatat aaatttaaac gttttaacgc tatcggtcat
     1501 tcaaatggtg gcttatcatg gactattttc ctagaagatt attacgactc tgatgaattt
     1561 gatatgaaat cattgttaac aatgggaaca ccttttaact ttgaagaaag taacacctca
     1621 aatcatactc aaatgcttaa agatttaatc agtaataaag gaaatattcc atcaagtctc
     1681 atggtataca atttggcagg aactaattca tatgatggtg ataaaattgt tccatttgct
     1741 agtgtggaga ctggtaaata tattttccaa gaaaccgcta aacactatac ccaactaaca
     1801 gtaactggta ataatgctac acattctgac ttgcctgata atcctgaagt tatccaatat
     1861 gtcgcagaaa aaattcttaa aaatgagaaa ggtaaattac caaaacctca ctaaagctgt
     1921 aaaatagcaa cgcccctgct attttgatat aatatattgc gaattaaact aaataaaaaa
     1981 ggatattatt atgaaactca tttcatggaa tattgattcc ctcaatgcgg cattaacaag
     2041 tgaatcaaca cgtgctttaa tgtcacgcca agttattgac actttagttg ctgaagatgc
     2101 cgacatcatt gcgattcaag aaactaaact gtctgccaaa ggacctacta aaaaacattt
     2161 ggaagtactt gaaacttact tcccagaata tgatttggtt tggcgatcat cagtcgaacc
     2221 tgctcgtaag ggctatgctg gtacaatgtt cttatatcgt aaagggttaa atcctattgt
     2281 tagcttccct gaaatcgatg ctcctactac aatggataat gaaggtcgca ttattactct
     2341 cgaattggaa aactgctata tcacacaggt ctatactcca aatgcaggtg atggcctaaa
     2401 gcgcttagct gaccgtcaaa tatgggatat taaatatgct gagtatttag caacattgga
     2461 tagtcaaaaa cctgttctag caacaggtga ctataacgta gcccacaagg aaatagacct
     2521 agctaatcct agcagtaatc gtcgttctgc tggattcaca gatgaagagc gccaaggctt
     2581 tacaaatcta ctagccaaag gttttacaga tacttttcgt taccttcatg gcgatgttcc
     2641 aaatgtttat agctggtggg cacaacgaag tcgtacatcc aaaatcaaca atacagggtg
     2701 gagaattgat tattggctaa caagtaaccg tgtcgcagat aaaattacca agtcagaaat
     2761 gattcattct ggtgatcgtc aagatcatac accaattatt ttggagattg aactataaac
     2821 cccaaaaaag atgattgagt tataactcaa tcatcttttt atactaagtt gaggtcttta
     2881 tacttagtac ggtagccaat ctgaagaatt ttattatctt taactagcaa tggacgctta
     2941 ataagcatcc catccgatgc caataagtcc aaagcttggt caagagttaa atcatcaaat
     3001 ttatctttta agcctaattc tctgtaagaa tttcctgaag tattgaaaaa ctttttaagg
     3061 tcataaggcg aattctctaa caattctttt aataacgaag ctttgggagg attgcttttt
     3121 atatcaatcg cttcaaaagt caagccgagt tcagttaact ctttttttgc tgaacgacaa
     3181 gtggtacatt tggggtattc ataaaacgta tacatattat ttctcctttt cgtattctaa
     3241 taaccaagat tttctttcta agccaccagc ataaccagtt aattgaccat atcttcctaa
     3301 aacacggtga caaggtatga ttagagaaat aggattttta cctattgctc ctccaacagc
     3361 ctgtgcagat tgacaattta gctctttcgc taattctcca taagtttttg tatctccaaa
     3421 agggattttc tgtaattcct gccatactct cgtctgaaat gctgtccctc tcaatgataa
     3481 tggaatacta ggtaaatttt gctgtgaaaa ataagcatct aaccacttct taccaagtaa
     3541 agtataggaa tttgacatat taacaatttc ctcttggttg actgctagca tatcatattt
     3601 ttgcccaaca aaataaagcc ctgataaacc tagattgtct gctagaaggc gaatttctcc
     3661 aagaggggac tgatagaatt cttgatataa catatgataa gcataactta aaaagccatg
     3721 aaaagcaatt catgactttt tttagttctt tattgttttt ttgcaattaa acgtacacgg
     3781 acgatattct catcaccttc aaattcttca attaaagtag aaatcttgtt attatccgtt
     3841 tcatctaaat ctattagtgt gtaggcatag tcaccctttg aacggttaat aatgttatca
     3901 atatttatac ctaactctga tacagcagtt gaaattttag ccacaatgtt aggaacattc
     3961 ttgttaatta aagtaatacg aaatggtgcg gtttgaattt gatgtacatt ggggaaatta
     4021 actgaattag tgatttctcc tgtctccata aaacaacgaa tggtttgact tgccataata
     4081 gcacaattta gctctgcctc atctgttgat ccgcctacat gaggaaagac tgtaattcct
     4141 ttttggttta ataattcttt gtctccaaaa tcagtaatat agcgcttgac aacaccagtt
     4201 tctatcgctt caaatagctc ttggttattg actaattctg cacgagcaaa gttgataatc
     4261 gtagttcctt ttttcatgat tgaaaaagct tccgcatcaa aagtatgctt agtttcattt
     4321 gttaagggaa catgaattgt gatatagtca caagtttcaa aaatatcctt aatctcttta
     4381 accctttgaa catggcttga aatattccaa gctgtttcaa ttgaaacata gggatcataa
     4441 ccaagaactg tcattcctaa gcgtctagca tcattcgcaa ttctagctcc aatggcacca
     4501 aggccgataa ctcccaattt ttttcctgca atttcattac cagcaaaagc tttctttcct
     4561 gcttcaattt gtttgggaat atctgttcca gttagagtat taacccatcg gttagctcct
     4621 aaataatcac gagctgaaag caataaggca gcaatgactg cttcttttac agcattagca
     4681 tttgcacctg gggtattaaa gacgactatt ccctgtgcac ttgcctcttc aataggaata
     4741 ttatttgttc ctgcacccgc cctagcaata gctttgaggt tacttgggaa atcctgatta
     4801 tgcaaatttt gactacgaat aatataggca tcaggatttt ctgacatatc cccatcaatc
     4861 tggaaacgat tccctaactc ttgtaaacca atttgattaa tattattaaa tgttttgaca
     4921 ctaaaaacca ttctttttca ctaaggttat ctctttagtc atcattgact aaaataaccc
     4981 ctttcttttt ttcttacatg ggatttcttt catcaatctc acgatagatg ctcgtttctc
     5041 ccttttcttt tatcttctga taagccaatc gtaccccatc tagaggtact ttaccacaat
     5101 attgatagcc taatttattt agaatatgct gcatagtgac atttttttca tgagtatcgc
     5161 aacgaaaatc aggtccttta tgaccttcaa ttaacccttg aaggaatgtc tgtgctagtc
     5221 ctctaccacg aaattgattt gaaatagcaa tacgatgaaa tgttagatag cgatggttat
     5281 catggagcca ttttccctca taaatagcat cataaacctc ttcatgacca tcaatgacag
     5341 ccgcataagt cgctagcatc ccatcttcaa taccaaccca agcataaccg ttcaagatat
     5401 catcaataat atcgttacga ttagggtatc catcttcttt ttgccattga tcacttcctg
     5461 ttttagcaat ttcggcacgc gcttcctcaa taagaagcat aatttggtca atttcatttg
     5521 gaaaagctaa tctgatttgc attatattac ccacttttat atattataga ctataataac
     5581 ggtttaattt tctttcgtga taacatttac tatgcatttt ctgcttcaaa tgttttcata
     5641 aaatcaatta attcgatgac accttgttta ggaaaggcat tataaaggct agccctcata
     5701 ccacctaccg aacgatgtcc tttaatattt ttaaatccac gttctgtcgc ttcctttaca
     5761 aatttttcat ctaaatcttt attaatagtt atgaaaggaa tgttacatag agaacgactc
     5821 tttttatctc tgacaggatt actataaaat tctgaggaat caatataatc atagagaagt
     5881 cctgattttt cacggttagc tttctccata gcatcaacac cacctagact tttcacccat
     5941 tcaaagacta atttagcaat gtaaatactg taagcaggcg gcgtattata aagagaacca
     6001 gcatctgact gaatcttata atccaacatt gatgataaag ttggttcctc attaatcata
     6061 tcttcacgga taataactac cgttacacca gcaggtccaa tatttttttg agctcctgca
     6121 taaatcattg caaaatcttc tactttatat tttacagcta agatattaga agacatatcc
     6181 gcaataacag gcactccatt agttttagga atatcataaa gcgatgttcc ttcaatggta
     6241 ttatttgttg tcacatgaac ataggcagct ttaggatcaa tctctttttc atcaaactct
     6301 ggaatataat cataaactga ttcttctgat gaggctaata gaattggttc aaaaggaatt
     6361 gtttttgata gttttacagc ctctgtataa gcttttttcc cccacgaacc agcaacatga
     6421 tagtaagctt tacgccctct ggcaatattc aagggaatca tactaaactg taaggatgca
     6481 ccaccttgta aaaagattac tttataatta tccggaattc ccatcaaatc tcttaagtaa
     6541 cgctcagcac ctttgataat atcatcaaac tctttggagc ggtgcgatac ctccaaaaca
     6601 ctcatactgg acccttgata attaaggagt tcagattggg cttttacaag cactggtttt
     6661 ggtaagactg caggacctgc agaaaaatta taaattgtca tataacacct ccatataatt
     6721 gtgtaagatt atagcacagt attttgaata ttgtaaagat ttttttgatt aattttgctt
     6781 tttattcgtg atataatggt aaaggcgaac aatcaagttt aaaggagtaa tttatgattt
     6841 tgagggaatt ttgtgcagag aatttaacag acttaaccag attagataaa gctattattt
     6901 cacgtgtcga gctttgtgat aaccttgctg tcggaggcac tactcctagc tatggtgtta
     6961 tcaaagaagc taatcaatat ttacatgaaa aaggtattag tgtcgctgtt atgattcgtc
     7021 cccgtggagg aaattttgta tacaatgatt tagaattacg tattatggaa gaggacatcc
     7081 tacgtgctgt tgagttagaa tcagatgctc ttgttttggg aatattaact tcaaataatc
     7141 atattgatac tgaagccatt gaacaacttc tacccgctac ccaaggttta ccacttgttt
     7201 ttcatatggc atttgatgtt atcccgaaat ctgaccaaaa aaaagcaata gatcagttag
     7261 ttgctttagg ctttacaagg attctcctac atggttcatc taatggcgaa ccaattatag
     7321 aaaatatcaa acatatcaaa gcactagttg aatatgctaa taatcgtatt gaaattatgg
     7381 ttggcggtgg tgtgactgct gaaaactatc aatatatttg ccaagaaact ggggttagac
     7441 aagcccatgg tactagaata actcaataaa aaattcgaaa caggactata agtttcttta
     7501 gtatatttac tactaagttc tttcatgttt tgtaaaacct gaaagaaccg gaataagagc
     7561 ataccatctt ttataacctg ctctcagaaa taaaatccaa tttgcaatgg tattcaaaag
     7621 agctgaaatt aaaaatc
//
LOCUS       DATRNK010000073         7466 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.073, whole genome shotgun sequence.
ACCESSION   DATRNK010000073 DATRNK010000000
VERSION     DATRNK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.073"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..755)
                     /locus_tag="VBC52_001758"
     CDS             complement(<1..755)
                     /locus_tag="VBC52_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001909609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HEO6883322.1"
                     /translation="MVNNRKIETLGLSYLRTVLDKNNYLQTYFDENDKTPLWDGEIHL
                     LRAPSEKKSDIIGKVPVQIKATQQKKKNNRFLISLSDLELYQKNGGFVLFVVWIDEEN
                     NIKEILYKSLPPLSIKNLIKRSKRKLTTGAPKKVSIEIHSLSENKVYPMLMDFINSSR
                     KQYSFATSRGLTMDKLPKEPKLKFYHYGKSPIDVFDYQEEHELFAYMVDDDTGIEIPI
                     ENPIKIVETAEKTDLTFTIGNYTIKNVVRCRFP"
     gene            complement(893..2878)
                     /gene="tkt"
                     /locus_tag="VBC52_001759"
     CDS             complement(893..2878)
                     /gene="tkt"
                     /locus_tag="VBC52_001759"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO6883323.1"
                     /translation="MTFDAVDQLAVNTVRTLSMDAIQAANSGHPGLPMGAAPMAYVLW
                     NKYMNINPKTSRNWTNRDRFILSAGHGSAMLYSLLHLAGYDLSIDDLKNFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKYNKPGFNIVDHYTFALNGDG
                     DLMEGVSQEVASLAGHLKLGKLITLYDSNDISLDGPTSMAFTEDVKGRFEAYGWQHIL
                     VKDGNDLDAIAKAIEEAKAETEKPTIIEVKTIIVFGSEKQGTSAVHGAPLGAEGIVFT
                     KNAYKWTYEDFIVPDEVKARFQEGLQARGEQAENAWNELFAKYEAEYPELAKGYKEVF
                     SNEPVSVELAAHELGSSKASRVSSQEAIQQISEQVASFWGGSADLSASNNTMVKVEKD
                     FQPGQYEGCNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQS
                     LPTMYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNAAWKHALAEI
                     DKPTMLVLTRQNLPVLEGTKELAEEGLNKGAYILSEAKGEMDGIIIATGSEVKLAMDT
                     QAALAEEGIHVRVVSMPSQNIFDAQSDAYKEEILPATITKRLAIEAASSFGWAKYVGL
                     AGKTLTIDTWGASAPGNRIFEEYGFTTENATVLYKAL"
     gene            complement(2927..3571)
                     /gene="fsa"
                     /locus_tag="VBC52_001760"
     CDS             complement(2927..3571)
                     /gene="fsa"
                     /locus_tag="VBC52_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO6883324.1"
                     /translation="MKFFLDTANVEAIKTINELGLVDGVTTNPTIISREGRDFETVIK
                     EICDIIDGPVSAEVTGLTANEMVDEARALSKWHDNVIVKIPMTAEGLKATSILSKEGI
                     KINVTLIFTVSQGLMAMKAGASYISPFIGRLEDIGTDPYQLIEALRDIIDIYGFGTEI
                     ISASIRSAAHVEAVAKLGSHIATIPETIFDKMVQHPLTKSGIDQFMNDWASFKK"
     gene            complement(3589..4278)
                     /locus_tag="VBC52_001761"
     CDS             complement(3589..4278)
                     /locus_tag="VBC52_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEO6883325.1"
                     /translation="MQKVEISPSLMCMNLDKFQEEICFLDSHVDSYHIDIMDGHFVPN
                     ITLSPWFVEEVRKLSSLPMSAHLMVKDQPFWVQQMIDIKCEWICMHAEVINGLAFRLI
                     DQIHNEGLKAGVVLNPETPIEVIMPYIELLDKVTIMTVDPGFAGQRFLDSTLDKIVDL
                     RRLREENNYNYLIEMDGSSSRKTFKKIDEAGPDIYIVGRSGLFALTDNISESWDIMTN
                     DYFEMTGKKLN"
     gene            complement(4290..5393)
                     /locus_tag="VBC52_001762"
     CDS             complement(4290..5393)
                     /locus_tag="VBC52_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEO6883326.1"
                     /translation="MKDMIKKANFKGHLLTAISYLIPIVCGAGFLVAIGMAMGGRAQD
                     ALVIGNFSFWDTMATMGGKALGLLPVIIATGIAFSIAGKPGIAPGFVVGLIANAISAG
                     FIGGILGGYIAGFIALGILKNVKVPNWAKGLMPTLIVPFFAALLSSLIMIYLIGGPIS
                     AFTGWLTDFLKSLGNTSNLVMGAVIGVLSGVDLGGPLNKTVYAFVLTLQAEGVKEPLT
                     ALQLVNTATPVGFGLAYFIAKLFKKNIYTNDEIETLKSAVPMGIVNIVEGVIPIVMNN
                     LMPAILAIGVGGAVGGAVSMTMGADSAVPFGGILMIPTMTRPIAGICGLLSNILVTGL
                     VYSLAKKPVDRNEVTIVSAEDEEDIALDDIEIM"
     gene            complement(5406..5855)
                     /locus_tag="VBC52_001763"
     CDS             complement(5406..5855)
                     /locus_tag="VBC52_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6883327.1"
                     /translation="MEARDAIKRKLIKTNMNVTTKDEAFSELVSLLKNEGFVSDEGGF
                     IKDIYLRESEGQTGIGNYIAIPHGKSSHVTEPAVVIGINSSEIPWETLDDNGVKVIVL
                     FAVGNDSIAAQEHLKILSLFARKLGNDQIVEKLINAKNEDDVIEAFS"
     gene            complement(5869..6180)
                     /locus_tag="VBC52_001764"
     CDS             complement(5869..6180)
                     /locus_tag="VBC52_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEO6883328.1"
                     /translation="MKIVGVAACTVGIAHTYIAQEKLENASKAAGHDVKIETQGTIGI
                     ENQLTSEEIAAADIVLLAVDVKIAGEERFEGKKVVKVPTEVAIKSPNKLIAKLVEVAS
                     N"
     gene            complement(6194..>7466)
                     /locus_tag="VBC52_001765"
     CDS             complement(6194..>7466)
                     /locus_tag="VBC52_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6883329.1"
                     /translation="LDTYIESLLNIYIIFVSQLLTGNHFEKTINPLDKKHHAFYVSTA
                     VTLLHKASLRLGFEYTNSDIEYLSQMLLNYRFEPIPVQQNDDKFVTALLEDVSKAMSI
                     NFLEDQYLFDQLSQHIPAMLYRLKYHTHIQNPFTDKIKLEFAITYNVLWLVLQDLGQE
                     LDVVFNEDEVAYLTIYFQLSIEKIGKSRKILIVCQTGIVTSELLINRIKKVVPSLDQV
                     EIASLSEMYEKDLTCYDFVLTTIETNLDLPNLYYVSPFIKNEEITQLLTGNPYSNNVT
                     IGKKDQIEANYYIPMLNQDFKTKEELLNSVGQNLVEMGYVQKEFVASLLDREKMGSTE
                     FPLGVALPHGRPEFVLKTVVVFIQNTHKIKWKESLVDTVFLIALSRDDIKNVKDIILK
                     INKTISNEEVLRQLKSETRELEVYKLLNQAD"
BASE COUNT         2258 a         1493 c         1152 g         2563 t
ORIGIN      
        1 tttggaaaac gacatcgaac cacatttttt atagtataat ttccaatagt aaaggtaaga
       61 tctgtctttt cagcagtttc aacaattttt attggattct caattgggat ttcaatacct
      121 gtatcatcat ctaccatata agcaaaaagt tcatgttctt cttgataatc aaaaacatca
      181 attggacttt ttccgtaatg ataaaatttc aattttggct ctttcggcag tttgtccata
      241 gtcaatccgc gactagtagc aaaactatac tgcttgcgac ttgaattgat aaaatccatc
      301 agcattgggt aaaccttatt ttctgataag gaatggattt caattgacac ttttttaggt
      361 gcccctgtag tcaattttct tttacttctc tttataagat tttttattga aagaggtgga
      421 agagatttgt ataatatctc ttttatatta ttctcttcat caatccatac tacaaacagt
      481 acaaatccac catttttttg atacaattct aaatcactca gacttatcaa aaatctatta
      541 ttctttttct tttgttgagt tgctttaatt tgaacaggaa cttttcctat aatatcagac
      601 tttttctcgc taggtgctct tagaaggtga atctctccat cccaaagtgg tgttttatca
      661 ttctcatcaa agtaagtttg taagtagttg ttcttatcaa gaacagtacg taaataagac
      721 aatcctaacg tttctatttt tctattgttt accaaatcac acctcgataa gcttctgact
      781 acattatatc atgtatctaa ataatatata gaatttctca gattcttatt tcataacttt
      841 ttaaacagaa aaaaacaaga tgatattgaa tcatcttgct ttttgattat ttttataatg
      901 ctttatatag gactgtagca ttttctgttg tgaaaccata ttcttcaaag atacgatttc
      961 ctggtgccga agctccccat gtgtcaatgg ttaaggtttt gccagctaag cctacatatt
     1021 ttgcccaacc aaagcttgat gctgcttcaa ttgccaagcg ttttgtgatt gtggctggta
     1081 aaatctcttc tttataagcg tcgctttgcg catcaaagat attttgtgat ggcattgata
     1141 caacacgaac atggatccct tcttctgcta gcgcagcctg tgtatccatg gctaatttta
     1201 cttcagatcc cgtcgcaatg atgataccat ccatttcgcc tttggcttct gacaagatat
     1261 aagcaccctt atttaaacct tcttctgcta attcttttgt tccttcaaga actggcaagt
     1321 tttgtcgtgt taacactagc attgttggtt tatctatttc tgctaaggca tgtttccatg
     1381 cggcattggt ttcattacca tccgctggac gaatgacgtt caagtttggc attgaacgga
     1441 cacttgctaa ctgttcaatt ggttcatgag ttggaccgtc ttcaccaacg gcaatggagt
     1501 catgtgtcat gacatacatg gttggtaagc tttgcagtgc tgccatacgt actgcaggta
     1561 atagatagtt tgagaagacg aagaatgtac caccataaac gcgagtacca ccatgaagag
     1621 caataccgtt catcgcagct gccatggcaa attcacgtac accaaaccag atattgcagc
     1681 cttcatattg acctggttgg aagtcttttt caactttaac cattgtgttg tttgaggctg
     1741 aaaggtctgc cgaaccaccc cagaatgatg cgacttgttc agagatttgt tggatagcct
     1801 cttgacttga aacacggcta gcttttgatg acccaagttc atgcgcagct aattctactg
     1861 aaactggttc atttgaaaag acttctttat agcctttagc aagttctgga tattccgctt
     1921 catattttgc aaagagttca ttccaagcat tttcagcttg ttcaccacgt gcttgtaatc
     1981 cttcttggaa acgagctttc acttcgtcag gaacgatgaa atcttcatag gtccatttgt
     2041 aagcattttt agtaaagaca atgccttctg ctccaagtgg ggcaccatga actgctgaag
     2101 ttccttgttt ttcagatcca aaaacaataa ttgttttaac ttcaataata gttggttttt
     2161 cagtttcagc ttttgcctct tcgatagctt tggcaatagc atctaagtca ttgccgtctt
     2221 taacgaggat atgttgccat ccataagctt caaaacgtcc tttaacatcc tcagtgaagg
     2281 ccatagatgt agggccatct agagaaatgt catttgaatc atataaagtg attaattttc
     2341 caagttttaa gtgacctgct aaacttgcga cttcttgact gacaccttcc attaagtcac
     2401 catcaccatt taaagcaaaa gtataatggt caacgatatt gaaacctggt ttgttgtatt
     2461 ttgcagcaag gtgagcttct gccattgcca tacctacagc gtttgcgata ccttgtccta
     2521 gcggtcctgt tgttgcttct acaccatctg tatgattgac ttcaggatgt cctggcgttt
     2581 ttgaacccca ttgacggaaa tttttcaaat catcgattga aaggtcgtaa ccagctaaat
     2641 gtaataagct gtatagcatt gcagatccat gtcctgcaga aagaataaaa cggtctctgt
     2701 ttgtccagtt acggcttgtt ttaggattaa tgttcatgta tttattccat agaacataag
     2761 ccattggagc tgcccccatt ggtaaaccgg ggtggcctga attagctgct tggatagcat
     2821 ccattgaaag cgtacggaca gtgtttactg ccaactggtc aactgcatcg aatgtcataa
     2881 gtaaaacctt tctgttttta gtaatataaa aaggggattc ccctttttat tttttaaatg
     2941 aagcccaatc attcataaat tgatcaatac cactttttgt taaggggtgt tgtaccatct
     3001 tgtcaaaaat ggtttccgga attgttgcga tatgtgatcc caatttagca actgcttcaa
     3061 catgagctgc actgcggatg ctagcagaaa taatttctgt gccaaaacca tatatgtcaa
     3121 taatgtcgcg aagagcctca attaattgat aagggtctgt accgatatct tctaaacgcc
     3181 caataaatgg actgatatat gatgcgccag ctttcatggc cataagacct tgtgaaaccg
     3241 taaagataag ggtgacattg attttaatgc cttcttttga caaaatactg gttgctttca
     3301 atccttctgc agtcattgga attttaacaa taacattgtc atgccattta gatagtgcac
     3361 gcgcttcgtc caccatttca ttagctgtta atcccgttac ttctgctgaa actggaccat
     3421 caatgatgtc acaaatttct ttaataaccg tttcaaaatc acgaccttcg cgagagataa
     3481 tcgttggatt tgtcgttaca ccatccacca aaccaagttc attgatagtt ttaatagctt
     3541 caacattagc tgtgtctaaa aaaaatttca tttctatcct ctatttatct agtttaattt
     3601 ttttccagtc atttcaaagt agtcattggt cataatgtcc catgattctg aaatattatc
     3661 tgttaatgcg aataaaccac ttcgtccaac aatgtatata tcagggccag cctcatcaat
     3721 ttttttaaat gttttacggc tagatgaacc atccatctca attaagtaat tataattatt
     3781 ttcttctctc aatcttctta gatctacaat tttatctaaa gttgaatcta aaaacctttg
     3841 tccagcaaaa ccaggatcca ctgtcataat agtaacttta tctaacagtt caatataggg
     3901 cattattact tcaatagggg tttcgggatt taaaactacc cctgctttta aaccttcatt
     3961 atgaatttga tcaattaacc taaacgccag accatttatc acttcagcat gcatacaaat
     4021 ccattcacat tttatatcaa tcatttgttg cacccaaaac ggttgatctt ttaccatcaa
     4081 atgagctgac attggtaagg aggatagctt tctaacttct tccacaaacc aaggagacag
     4141 tgtaatatta ggtacaaaat gtccatccat tatatcaata tggtaggaat caacatgtga
     4201 atctaaaaaa cagatttctt cttggaattt gtcaagattc atacacatca acgagggaga
     4261 tatttctact ttttgcatca tagttctcct tacatgatct cgatatcatc aagtgcgata
     4321 tcttcttcgt cttcagcaga aacaattgta acttcattac gatcaactgg ctttttagcc
     4381 aatgaatata ctaatcctgt taccaagata ttagataaca agccacaaat tccggcaatt
     4441 ggcctagtca ttgtaggaat cattaaaatt ccaccgaatg gcactgcaga atcagctccc
     4501 attgtcattg aaacagctcc tcctacagct ccaccgacac caattgctaa aattgcaggc
     4561 attaagttgt tcattacaat agggattacc ccttcaacaa tattcacaat ccccattggc
     4621 actgcagatt ttaaggtttc aatctcatcg ttagtataaa tgtttttctt gaataattta
     4681 gcaataaaat aggctaaacc aaatcctact ggtgtcgctg tattaactaa ttgtaaagca
     4741 gtaagtggtt cttttactcc ttcagcttgt aaggtcaata caaatgcata aacagtttta
     4801 ttcaatggtc ctccaaggtc tacccctgaa aggacaccaa taacagcacc cataacaaga
     4861 tttgaggtat ttcctaagct ttttagaaaa tcagttaacc aaccagtaaa ggctgaaatg
     4921 ggaccaccaa tcagataaat catgataaga ctactcaata aagctgcaaa aaatggaaca
     4981 ataagtgttg gcatcagtcc ttttgcccaa tttggtactt ttacattttt taaaatacct
     5041 aaggcgataa atcctgcaat atagccacct aaaataccac caataaaccc tgcactgata
     5101 gcgtttgcaa tcaatccgac aacaaaccct ggggcaatcc ctggtttacc agcgattgaa
     5161 aaagcaatac cagtcgcaat aataacaggt aacaaaccga gagctttacc acccattgtg
     5221 gccatcgtat cccaaaaaga aaaattgcca ataactagtg catcttgtgc tctaccgccc
     5281 atagccatac caatagcgac taagaaacct gctccacaaa caattggaat taaataagaa
     5341 attgctgtta gcaagtgacc tttaaaatta gcctttttaa tcatatcttt cattattatc
     5401 tccttttacg aaaatgcttc tataacatca tcttcatttt tagcattaat taatttttct
     5461 acaatttgat catttcctag ttttctagca aatagtgata atatttttaa atgttcttgt
     5521 gcagcgattg aatcatttcc tactgcaaat aaaacaataa cttttacacc attatcatct
     5581 aaagtttccc atgggatttc tgaagaatta atcccaatta cgactgcagg ctctgttaca
     5641 tgtgagcttt taccatgcgg aatggcaata taatttccaa tgccagtttg tccttcagac
     5701 tctctaagat agatgtcttt tataaatcct ccttcatcag aaacaaaccc ttcatttttt
     5761 aaaagcgaca ctaattctga aaatgcttca tcttttgtgg tgacattcat attagtttta
     5821 attaatttac gtttaatagc atctcttgct tccattgggc aaccctttct aatttgacgc
     5881 aacttccaca agttttgcga ttaatttgtt aggtgactta atcgcaactt ctgttggaac
     5941 ttttactact tttttccctt caaatctttc ttcaccagca atttttacat caacagctaa
     6001 caaaacaata tcagctgctg ctatttcttc tgatgttagt tgattttcaa taccaattgt
     6061 cccttgtgtt tcaatcttga catcatgacc agctgctttc gaagcatttt ccaatttttc
     6121 ttgagcaata taagtgtgag caattcctac tgtacatgct gctactccaa caattttcat
     6181 aatatttcct ccattaatcg gcttggttta ataatttata aacttctaat tcccgtgttt
     6241 cactttttaa ttgtcttaat acttcttcat tacttattgt tttattaatt ttcaaaataa
     6301 tgtcttttac gtttttaata tcatctcttg acagcgcaat gagaaatact gtatcgacca
     6361 atgactcttt ccatttaatt ttatgagtat tttgaataaa aacaaccact gtttttaaga
     6421 caaattctgg ccttccatgc ggtaaagcga ctcccaatgg aaactctgta cttcccattt
     6481 tttccctatc aagtaaacta gcaacaaatt ctttttggac atatcccatt tctactagat
     6541 tttgtcccac agaatttaat aattcttctt tcgttttgaa atcttgatta agcatcggaa
     6601 tgtaatagtt agcttcaatt tgatcttttt tcccaatagt cacgttatta ctataagggt
     6661 ttcctgttaa taattgggta atttcttcat ttttgataaa tggtgagaca tagtaaagat
     6721 taggcaaatc taaattggtt tcaatagttg taaggacaaa gtcataacaa gtcaaatctt
     6781 tctcatacat ctcacttaag gatgcaattt caacttgatc tagtgaaggc acaacttttt
     6841 ttattctatt aattagtaat tcagaagtca caatcccagt ttgacatact attaagattt
     6901 ttctactttt cccaattttt tcaatggaaa gttgaaaata aatggttaag taggctactt
     6961 catcttcatt aaaaacaaca tctaactctt gacctaagtc ctgtaacacc aaccaaagta
     7021 cattatacgt tatggcaaac tccaatttta ttttatctgt aaatggattt tgtatatgcg
     7081 tatggtattt caatcggtac aacatggctg gtatatgttg actaagttga tcaaaaagat
     7141 attgatcttc caaaaaattg atggacatag cctttgaaac atcctccaat agggcagtga
     7201 caaatttatc atcattttgc tgaactggta tcggttcaaa cctataattc aataacattt
     7261 gagaaaggta ctcaatatca ctatttgtat attcaaaacc taatcgtaag gaagctttat
     7321 gtaataatgt tacagctgta ctcacataaa aggcatgatg ctttttatcc aaagggttta
     7381 tagtcttttc aaaatgattc cccgtaagta attgactaac aaatatgata tatatattta
     7441 agagactctc aatataagta tctaat
//
LOCUS       DATRNK010000074         7252 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.074, whole genome shotgun sequence.
ACCESSION   DATRNK010000074 DATRNK010000000
VERSION     DATRNK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.074"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(611..3265)
                     /locus_tag="VBC52_001766"
     CDS             complement(611..3265)
                     /locus_tag="VBC52_001766"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEO6883330.1"
                     /translation="MSKELSPKYNPAEVEEGRYQTWLDQDVFKPSGDTEAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQG
                     ISRYDLGREKFLDKVWEWKDEYAATIKSQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYNKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGQNVILPIINKPIPIIADEHADPEFGTGVVKIT
                     PAHDPNDFAVGQRHNLPQVNVMNDDGTMNELADEFNGMDRFEARKAVVAKLESLGNLV
                     KIEKMTHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FMSWMENVHDWVISRQLWWGHQIPAWYNVNGEMYVGEDAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPDTEAADFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFSNVL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDQARENVEKVVNSQVGNVTDRWILHNLNETVGKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVVTRSVLLYTLDQI
                     LRLLHPIMPFVTEEIFGQYAEGSIVLASYPQVNATFENQTAHKGVESLKDLIRSVRNS
                     RAEVNVAPSKPITILVKTSDSELESFFKDNSNYIKRFTNPETLEISSAITAPELAMTS
                     IITGAEIFLPLADLLNVEEELARLEKELAKWQKELNMVGKKLSNERFVANAKPEVVQK
                     EKDKQTDYQTKYDATIARIEEMKKLNND"
     gene            complement(3265..3831)
                     /locus_tag="VBC52_001767"
     CDS             complement(3265..3831)
                     /locus_tag="VBC52_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6883331.1"
                     /translation="MNIIIIGAQASGKMTIGQEIAKQTGMTLFHNHDSIDFVLRFMPW
                     SPDSIALTESIRFKFFETFAKTGQEMIFTIVIDFNDSRDVVFLEKIQTVFQSHNQEVL
                     FVELETELSERLKRNRTENRLKHKPSKRDIKWSENDICSTMDYAIFNPEVAPEALTYY
                     HKINNTCLTATETAYLIIQKINQIKEKN"
     gene            complement(3828..4412)
                     /locus_tag="VBC52_001768"
     CDS             complement(3828..4412)
                     /locus_tag="VBC52_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6883332.1"
                     /translation="MIKILGEIVDNQLPVVETNRLLLRQRKLEDAKEIFEFVKLDEVS
                     YPAGFPAVKSLEEEITYIQEIYPTNLEKEKLPSGYAITLKGDDKVIGSVDFNHRHEDD
                     IFEIGYLLHPDYWGQGIVPEAASALVEIGFTLLGLHKIELGCYDYNKQSQAVARKLGF
                     TLEANIRDRRDAQGQRCGDMRFGLLRSEWEKQRR"
     gene            complement(4510..5078)
                     /locus_tag="VBC52_001769"
                     /pseudo
     CDS             complement(4510..5078)
                     /locus_tag="VBC52_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(5347..5658)
                     /locus_tag="VBC52_001770"
     CDS             complement(5347..5658)
                     /locus_tag="VBC52_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883333.1"
                     /translation="MAKRIKPNKKRELLKAKGINWKDVRLSQTLQEVASPKKKAPEAP
                     KVVDLLEGLTANKQDVLKEAGLVSLEAFAKVSEADVLALKGIGPAAIKQLVDNGAVFA
                     K"
     gene            complement(5767..6021)
                     /locus_tag="VBC52_001771"
     CDS             complement(5767..6021)
                     /locus_tag="VBC52_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEO6883334.1"
                     /translation="MSYEQEFLKDFEEWLQSQISINQMAMDSAKKVLEEDKDERAADA
                     YIRYESKLDAYRFLQGKFNNYHNQKSFHDLPDGLFGQRHY"
     gene            6332..7166
                     /locus_tag="VBC52_001772"
                     /pseudo
     CDS             6332..7166
                     /locus_tag="VBC52_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018434.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
BASE COUNT         2219 a         1500 c         1225 g         2308 t
ORIGIN      
        1 aaacattttt acataaaagc taatgatagg cctattttac taattccaaa gtgtctcgtt
       61 tcaaaaaaat ataaattctc tacatcacat ttcgtcaaaa ctataatatt cccaaataaa
      121 aaaaacttag aaaagtatca aggaataaat ggatatgata aaagtaatag acctaaacca
      181 gcaactcaga aacaacttat tgagcacgaa atacgttcac cttatctaaa ctgcccagat
      241 aagtggaaaa cttacgccat ggagcagcta ttacaaaatc acaattggta taatgaatat
      301 ttcctaaata tgaataattt tgctgataac catattatac ctgatgacga acttgatagt
      361 ttaaccaata actaaatatt taatactgag aatagtgtcc ttctctcaat tgagagaagt
      421 ttttttatta ttactatttt ttataacacc ttaccaaaac atcctttaaa attactatct
      481 tcattaggta tactctttaa ggaatacttt tcctttgatc tattttatgt acacttgtta
      541 acgttattac tatcaaatcc cccactatct tattctacta tactaaaaat acagcctctc
      601 ggctgcattc tcaatcatta tttaattttt tcatctcttc aatacgtgct atagttgcgt
      661 cgtatttagt ttggtagtcg gtctgtttgt ctttttcttt ttggacgact tctggtttag
      721 cgttagctac aaagcgctcg ttgctgagtt ttttgccgac catatttagt tctttttgcc
      781 atttagcaag ttctttttca agtcgtgcca actcttcttc aacgttaagg aggtcagcaa
      841 gtgggaggaa aatttctgca cctgtgataa tgctagtcat ggcaagttct ggggcagtaa
      901 tcgctgagct gatttcaagc gtctctgggt ttgtgaagcg tttgatgtag ttactattgt
      961 ctttgaagaa gctttcgagt tcgctgtcac ttgtcttaac caagatagtg attggtttag
     1021 atggagcaac attgacctct gctctgctat ttcgcactga acgaatcaaa tctttcaagc
     1081 tttcaacgcc tttgtgagct gtctgatttt caaatgtcgc gttaacttgt ggataacttg
     1141 caaggacaat tgatccttct gcgtattgtc caaaaatttc ttctgtcaca aatggcatga
     1201 ttggatgaag aagacgcaag atttgatcta aggtataaag aagaacagaa cgtgtaacaa
     1261 ctttctcctc ttcattgtca ctataaagaa cctcttttgt taactcaacg taccaattag
     1321 caaattcttc ccagataaag ttgtaaagga tgtgaccagc aacaccaaat tcaaacttgt
     1381 caaagttttc agtaacttta cccacagtct catttaaatt gtggagaatc caacggtctg
     1441 ttacatttcc aacttgacta ttaacaactt tttccacatt ttctctggct tgatccaacg
     1501 ttaaaccttc gttattcata aggatatagc gtgaaatgtt ccaaatctta ttaatgaaat
     1561 tccacgaagc atccattttt tcgtatgaaa aacgcacatc ttgaccaggg gctgaaccat
     1621 ttgagaggaa ccaacgaagg gcatccgcac catatttctc aatgacatcc atcggatcaa
     1681 tcccgttgcc aagtgattta gacattttac gtccttcttc atcacggatg agtccatgaa
     1741 taagaacatt tgagaatggt tggcgtcctg taaattcaag agattggaaa atcatgcgcg
     1801 atacccagaa gaagatgata tcgtatccag ttaccaaggt tgatgttggg aagtagcgtt
     1861 tgaagtcagc tgcttcggtg tctggccatc ccattgttga aaacggccat aaagcagaac
     1921 taaaccatgt gtctagaacg tcttcatcct gtgtccatcc gtcaccctct ggggcatctt
     1981 ctccaacata catctcacca ttgacgttgt accatgcagg aatttggtgt ccccaccata
     2041 attgacgaga gataacccag tcatggacat tttccatcca actcataaaa gtatcgttaa
     2101 atcgtggtgg ataaaactct accttatctt ctgtatcttg gttggcaatg gcattcttag
     2161 ccaattgatc catcttgacg aaccattgag tagataaacg tggttcaacc acaacgcctg
     2221 ttcgttctga atgaccaaca gaatgagtca ttttttcaat ttttactaag tttcctaacg
     2281 attctaattt agcgactact gccttacgag cttcgaaacg atccatacca ttaaattcat
     2341 ccgcaagttc attcatagta ccatcatcgt tcataacgtt aacttgtggt aagttgtggc
     2401 gttgacctac agcaaagtca ttagggtcgt gagccggcgt aatcttaacc acacctgttc
     2461 caaattctgg atcagcgtgt tcatcagcga tgattggaat aggtttattg atgataggaa
     2521 ggataacatt ttggccaatc aaatctttgt aacgtggatc ttctggattt accgctacag
     2581 ccacatctcc aaacatagtc tcaggacgag ttgttgctac ttctagggcg cgtgagccat
     2641 cttccagcat ataattcata tgatagaaag caccctcaac atccttgtga ataacttcaa
     2701 tatctgaaag ggctgtacga gctgctggat cccaattaat gatgaactcc ccacgataga
     2761 tccaaccttt attgtacaaa tcaacaaaga cttttcttac cgcttttgaa agtccctcat
     2821 caagggtaaa acgctcacga ctataatcaa ccgaaagacc cattttcccc cactgagatt
     2881 taatagttgc tgcatactcg tctttccatt cccaaacttt atctaggaat ttttcacgac
     2941 caagatcata acgtgaaata ccttgctcac gaaggcgctc ctcaacctta gcttgagtag
     3001 ctataccagc gtggtccata cctggaagcc agagtgtatc aaagccctgc atacgctttt
     3061 gacgaataat gatatcttgt agagttgtat cccaagcgtg acccaagtgc aatttacccg
     3121 taacgtttgg tggtggaatc acgatagaat aaggcttagc ctcagtatcc cctgatggct
     3181 tgaaaacatc ttggtcaagc caagtttgat aacggccttc ctcaacctcg gcaggattgt
     3241 attttggtga tagttctttt gacattagtt tttctccttg atttggttta ttttttgaat
     3301 aataagatat gctgtttcag tagcagttaa acaagtatta ttgattttat gataatatgt
     3361 aagtgcttct ggggctactt caggattaaa gatagcgtaa tccatggttg aacaaatatc
     3421 gttctctgac catttgatat cacgttttga aggcttatgc ttgaggcgat tttctgtgcg
     3481 attccgtttg agacgctcac ttagctctgt ttctaattct acaaataata cctcttggtt
     3541 gtgagattga aaaactgttt gaatcttttc tagaaatact acatctcttg aatcattgaa
     3601 atcaattaca atagtaaaaa tcatttcttg accagtctta gcaaacgttt caaaaaattt
     3661 aaagcgaata gactccgtta gagcaatact atctggagac catggcatga agcgtaaaac
     3721 aaaatctata gaatcatggt tatggaataa ggtcatgcct gtctgttttg cgatttcctg
     3781 accaattgtc attttcccag aagcttgtgc tccgatgata ataatattca tcttctttgt
     3841 ttctcccact cgcttcgcag taaaccaaag cgcatatcgc cacaacgttg cccctgggca
     3901 tctcttcgat cacgaatatt agcctctaaa gtaaaaccta atttgcgcgc aaccgcctga
     3961 ctttgcttat tatagtcata gcatcccagc tcaatcttat gtagtcctag cagtgtgaaa
     4021 cctatctcca caagggctga agctgcttct ggcacaatgc cttgtcccca ataatcaggg
     4081 tggagcaggt agccaatttc aaaaatgtcg tcttcgtggc gatggttaaa atctactgaa
     4141 ccaattacct tatcatcgcc ttttagagta atagcataac cagagggtaa cttttctttt
     4201 tcaagattag ttggataaat ctcttgaata taggttatct cttcttccaa gcttttaaca
     4261 gccggaaaac ctgctggata actcacctca tcaagtttga caaattcaaa tatctcctta
     4321 gcatcttcta acttgcgttg acgcaacaac agacgattcg tttccacaac aggtagctgg
     4381 ttatctacga tttctccaag tatcttaatc atttgtctcc tccatatcta cgatattatt
     4441 gatgacttag ctaagtgccc tgtacagcca aattagtcta tctagtaact tctatgaaaa
     4501 gtagaataat tactcccact cactcctcag caaaccatat atcaaattga cacagcgatt
     4561 gccttgatta tccttacgag cacgaatggt agcttcaagg gtgaatccta gtttttctgc
     4621 cacgcgccgg ctttgcttat tgtaatcata gcagcggatt tcgattttgt ggagattgag
     4681 gagggtaaaa ccgacttcaa tcaaagcggc gacagcttct gtcatgtagc catgacccca
     4741 gtagtcagga tgaagcagat agcaaatctc gaaaacatcg tcttcatggc gatggttaaa
     4801 ggcgcaagat ccgataatcc gatcacttct ttaacggtaa taccataacc agatggtaat
     4861 tttgcctttt ccagattttg atagtagcga ttttcaaaat aatcgtactc atcttctaaa
     4921 gaagctattg ggctgagtcc tgctggatag gcaacctctt ccaagcaaac gtaatcaaac
     4981 atggctggga catcccccac cgttcgctga cgcaagataa gccgatccgt ttctagtttt
     5041 ggtaatgctt tgccatcgaa atcttctaga atctgcatat acttcaatct cctcactcct
     5101 aaatacaaaa aatccctgtc aattctgaca gggacgaatt tcgtattacg atttccgcgg
     5161 taccacccaa attcggataa taacatccgc aactcgtttt gtttcaatta ttggacttag
     5221 caacaagctt tgacatttgt gcggatttct cagtatgacc gcttcctgtg acaaatggga
     5281 tttcaaaaca cctctaagtt aattttattc tagcaaaata agactacttt cgcaaggtga
     5341 atcaaatcat tttgcaaaaa cagcaccatt atcaactaac tgtttaatag ctgctggacc
     5401 aatacctttt agtgctaaaa catctgcttc acttactttc gcaaacgctt caaggctgac
     5461 aaggccagct tcttttaaaa catcttgttt attagcagtt aaaccttcta aaagatcgac
     5521 tacctttggc gcttcaggag cctttttttt cggtgatgcg acttcttgta aagtttgaga
     5581 tagtcgaaca tccttccaat tgatcccttt agctttcaaa agctctcttt ttttattcgg
     5641 tttaatacgc tttgccattg tataatgctt tctcctcatt tatgaaatct atcatagtat
     5701 ctaaaaacta ctataacaat gtatcataga aatgcctatt tgacaagctt tctagcgata
     5761 aaccttttaa taatgacgtt gtccaaaaag accatcaggc aaatcatgga aagatttttg
     5821 gttatgatag ttattaaatt tcccttgcaa aaagcgataa gcgtctaact tactctcata
     5881 acgaatatac gcatctgctg ctcgctcatc cttatcttcc tctaaaactt tctttgcact
     5941 gtccattgcc atttggttga tacttatttg cgattgtaac cactcttcaa agtcttttaa
     6001 aaattcttgt tcgtatgaca tgatattcct cttttttagt ttttttcatt ataacatata
     6061 aattaaatac tcaaaaaatc attatatcaa aaataaatta tgatttttct gacagatata
     6121 aaaacaacta gttttatcta aggaagacct tttgagatgt atggttctct atgccattct
     6181 agttttggca tcacacccct agttgaaaga aaacgctctc taaaattcat cacttatacc
     6241 attcaatcac tattactcct tgttatctta gcacctatgt gttatagtta agaaaaaatg
     6301 atattactat taaaagaaag aggtgatctt attgtcacat aaagaaattg gacatcaatt
     6361 cttagcacga ctaggaaaaa acgcttaaga ccaggaggtg tccaagcaac gaattggctt
     6421 atcaaaaaag gacactttag tgctgacagt cgtgttctag aagtagcttg taatcaatgt
     6481 accagttcca tcgacatcgc taaagcattt tcctgtcaca ttacagcgct tgatctagat
     6541 agtaaagtaa tagaaaaggc caaagaacat atcaaaaagg cccaattaga agaatttatt
     6601 gaagttattc ggggaaatga actcagactg ccctttcctg ataatagttt atcgttatca
     6661 acgaagcaat gttaacgatg cttaacaatt cagctaagga gaaggtcata aaagagtact
     6721 tacgtgtcct aaaacctggt ggacgtttac tcacacatga tgtttcttat caagatgaag
     6781 acactgccaa tctgatcgat caacttagac atactataaa tgttaacgtt gctcctctaa
     6841 aaatagatga ttggaaaaat ttattcttac aaaatggttt ttcaagttca gaatatcaat
     6901 ttggaaaaat gagtctcatg tccccattag gaatgattaa agatgaaggt ttattcaata
     6961 ccattaaaat catcagccga ggactgaaaa atgaacgatg ccctcatcca aaattaataa
     7021 atgttttgga aggaaaactt gaaattatcc ttggtgttca gaaacatatc cttgaaaaag
     7081 atagccttat tagcctctct ccagaagaaa cacaccattt aagagctatt gaaaatagta
     7141 aatttctcca aatagaatta gactaaaaaa acgactattt ttctaagtaa agaaatactt
     7201 gatttcgtca aaatcttata gtatcataga aaagtagccg ctatagttaa at
//
LOCUS       DATRNK010000075         7050 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.075, whole genome shotgun sequence.
ACCESSION   DATRNK010000075 DATRNK010000000
VERSION     DATRNK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.075"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..210)
                     /locus_tag="VBC52_001773"
     CDS             complement(<1..210)
                     /locus_tag="VBC52_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883335.1"
                     /translation="MLQLTKYFPLKPIYLALLVFQIYLLVFSWTMLGCAFLLFSFIFL
                     IYQYDRETIFKTIAIVIFFLFYFLWQ"
     gene            complement(194..847)
                     /locus_tag="VBC52_001774"
     CDS             complement(194..847)
                     /locus_tag="VBC52_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEO6883336.1"
                     /translation="MFEIVLEKIKSHKWETTGIIVGLLLFGILGLNHFGTHHKEDNLN
                     INLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKGAVNHPGVYSLPSQSRVTDAIKRA
                     GGLSNLADSKSVNLAQKLQDETVIYVAQKGEKITVVEEEKANNIATQGNSKGKINLNK
                     ADLSSLQTISGVGAKRAQDILDYRDSQGGFKTIDDLKNVSGIGEKTLEKLRQDVTID"
     gene            complement(947..1687)
                     /locus_tag="VBC52_001775"
     CDS             complement(947..1687)
                     /locus_tag="VBC52_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEO6883337.1"
                     /translation="MFYTYLRTLVMFLIWVANGNAHYHNEDKMLKDDENYILVAPHRT
                     FWDPVYMAFAARPKQFIFMAKKELFTNRLFGWWIKMCGAFPIDREKPGQDAIRYPVKM
                     LKNSNRSLVMFPSGSRHSKDVKGGVAVIAKMAKVRIMPAAYRGPMVFKNLLKGHRVDM
                     NFGNPIDVSDIKRMDAEGIAEVSRRIQEEFDRLDRENETYDDGKKLNPLTYIYRLPLA
                     IIAIVLLVLTLIFSYLASFVWDPQKHLK"
     gene            1822..2586
                     /locus_tag="VBC52_001776"
     CDS             1822..2586
                     /locus_tag="VBC52_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEO6883338.1"
                     /translation="MIDTILKENERIDQLFSTDVKIIQNKEVFSYSIDSVLLSRFPKL
                     PSRGLIVDLCSGNGAVGLFASTKTNATIIEIELQESLADMAKRSIKLNKLEKQVTMIN
                     DDLKNLLDHVQRSNVDLMLCNPPYFKASETSKKNLSPHYLLARHEITTNLREICQIAQ
                     HALKTKGRIAMVHRPDRFLEIIDTMRQFNLAPKRIQFVYPKLGKDANMLLIEAIKDGS
                     TEGMKILPPLVVHQDNGDYTETIFDIYFGENGKSHD"
     gene            2579..2845
                     /locus_tag="VBC52_001777"
     CDS             2579..2845
                     /locus_tag="VBC52_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO6883339.1"
                     /translation="MIKVPAYMYVLECSDGTLYTGYTTDVKRRLNTHNTGKGAKYTRA
                     RLPVKLLYSEAFNSKQEAMRAEALFKQKTRQAKLTYIKQHKNEQ"
     gene            complement(3030..4616)
                     /locus_tag="VBC52_001778"
     CDS             complement(3030..4616)
                     /locus_tag="VBC52_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6883340.1"
                     /translation="MKFTELNLSQDILSAVEKAGFVEPSPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIHTEDNTIQALIIAPTRELAVQSQEELFRFGRDKGVKVRS
                     VYGGSSIEKQIKALRSGAHVVVGTPGRLLDLIKRKALKLNHIETLILDEADEMLNMGF
                     LEDIEAIISRVPETRQTLLFSATMPDPIKRIGVKFMKDPEHVKIKATELTNVNVDQYY
                     VRVKENEKFDTMTRLMDVDQPELSIVFGRTKRRVDELTRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDHIDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVSPNEMGYLTIIENLTKKRMTGMKPATASEAFQAKKKVALKRIARDFEDQ
                     ELVSKFDKFKADALELATQYTPEELALYVLSLTVQDPESLPEVEITREKPLPFKPSGG
                     GFKGKGGRGNGRGGDRRRNDRGDRRGNRDRDDRGGRCDFKRRDDKFKKDNRRQENKKP
                     HKNTSSEKQTGFVIRNKGDK"
     gene            complement(4851..5681)
                     /locus_tag="VBC52_001779"
     CDS             complement(4851..5681)
                     /locus_tag="VBC52_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883341.1"
                     /translation="MKKLLSLTCLIMMSLCLVACTKQAMSSKKIKDKNSKEKVITVAT
                     YSKPTSTFLDLVKDNVKEKGYTLKVVMVSDYIQANIALENKEHDANLLQHEFFMSIFN
                     KENDGHLVSITPIYHSLAGFYGQHLKNIAELKDGAKVAIPSDPANMTRALLLLQEKKL
                     ITLKNTSKKTKAIEDIITNPKKLRIEPVALLNLNQAYFEYDLVFNFPGYVTKINLVPK
                     RDRLLYEKKPDIRFAGALVAREDNKNSDKIKVLKEVLTSKEIRHYITKEIPSEAAVAF
                     "
     gene            complement(5697..6359)
                     /locus_tag="VBC52_001780"
     CDS             complement(5697..6359)
                     /locus_tag="VBC52_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6883342.1"
                     /translation="MIDIIEKHGEGILKSFWETNLMLGLTLILCFLIAFPTGILLFSL
                     RKSYLIKHSLAYQLLNLFLGTLRSVPFLIFIFILIPLNRLIFGTSFGTIAAILPLTLV
                     SVSLYARYVEQALLNIPQVVVDRALSLGANKRQIIYYFLIPSIKIDLVLSFTATAISI
                     LGYSTIMGVIGAGGLGEYAYRFGYQEYDYPVMYLIVVLFIIYVFILQSLGYFIANRYS
                     RK"
     gene            complement(6352..>7050)
                     /locus_tag="VBC52_001781"
     CDS             complement(6352..>7050)
                     /locus_tag="VBC52_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6883343.1"
                     /translation="PKSQFRLDDISFNIEEGEIVGLIGSNGTGKSTILKMINGLIPYD
                     KGNIYYQGKEVKSFSDNKLRQMRKDIAYIFQNHNLLAGESVYYHLALVYKLNHQKVNH
                     DAINDILDFLGLMDLKQVKCHSLSGGQQQKVAIAMAVLQKPKLILCDEISSALDTNSE
                     KEIFNLLSDLREKYGISILMIAHHLSLLKQYCDRVMILDHQTIVDTVVPVKATLNQLK
                     SNYVDQVKEFLLHD"
BASE COUNT         2329 a         1328 c         1065 g         2328 t
ORIGIN      
        1 ttgccataaa aaataaaata agaaaaaaat tactattgct attgttttaa aaatagtttc
       61 acgatcatat tgataaatca gaaaaataaa agaaaataaa agaaaggcac aaccaagcat
      121 tgtccaagaa aacactagta agtaaatttg gaagaccaac aatgctaaat aaataggttt
      181 tagaggaaaa tacttagtca attgtaacat cttgacgtaa tttttctagt gttttctcac
      241 caatacctga tacgtttttg aggtcatcaa tagttttaaa accaccttga ctatcacgat
      301 aatctaaaat atcttgagct cttttagccc caacgcctga tatggtctga agacttgata
      361 aatctgcttt attaagatta attttcccct tactatttcc ctgagtagcg atattatttg
      421 ctttctcctc ttccacaaca gttatttttt cacctttttg agcaacatag ataaccgtct
      481 catcttgtaa tttctgtgct agattaacgg atttactatc agcgagattg cttagtccac
      541 ctgcacgctt aatcgcatct gttacacgac tttgagaagg caaactataa acacctggat
      601 gattgactgc cccttttacg tcaaccgtta cttgattaga tactttatct ttgacgtgac
      661 taatcattgg tacctttttt tctgttatag ttgatacttt tttctctaaa ttgatattta
      721 aattgtcttc tttatgatga gtgccaaaat gatttaaacc caaaattcca aataataaga
      781 gtcctacaat aattcctgtt gtctcccatt tatgcgattt aattttttcc aatactattt
      841 caaacataaa aatattcctc catttactta ttcgtaacta aagagatttt cctattaaaa
      901 accaaaaaaa ctatttacta aaaagtaaat agtttccatg taacccttat tttagatgtt
      961 tttgtggatc ccacacaaag cttgctaaat aactaaaaat tagagttagg acaagcaaga
     1021 caatagctat aattgctaaa ggtagacgat aaatataagt caatggattt aatttcttac
     1081 catcatcata agtttcattt tctctatcta aacgatcaaa ttcttcctga atacgacgag
     1141 atacttctgc aatcccttcg gcatccatac gcttaatatc agaaacatca atagggttac
     1201 caaaattcat atcaacacga tgccccttta atagattttt aaaaaccata ggacctctat
     1261 atgctgctgg cataattctg acttttgcca tttttgcaat aacagctacg cctcctttga
     1321 catctttgga gtggcggctt ccactcggaa acatcaccaa agaacggttg ctatttttca
     1381 gcatctttac aggatatcga atagcatctt gccctggctt ttcacgatca atagggaaag
     1441 caccacacat tttaatccac caaccaaaca aacgattcgt gaataattct tttttggcca
     1501 taaaaataaa ctgtttgggt ctagcagcaa aagccatgta cacaggatcc caaaatgttc
     1561 tgtgtggagc tactaaaata taattttcat catcttttaa cattttatct tcattatgat
     1621 agtgcgcatt accattagcc acccaaatca agaacataac taaagtacgt aagtaagtat
     1681 agaacatgtt tctcctctac ctttcatcaa gtaacttcta ttttatcatg cttccttttt
     1741 tttagaaaga attatcttaa aatcaacctc cacactttca aaactagtgc taggaagctg
     1801 taattttgct ataataagcc tatgatagat acaattttaa aagaaaatga acgcattgat
     1861 cagctttttt caactgatgt caaaatcatc caaaacaaag aagttttttc ctattccata
     1921 gatagtgttt tattgtctcg gttccctaaa cttccttctc gtggtcttat cgttgactta
     1981 tgtagtggta atggtgctgt cggtttgttt gccagcacca aaacaaatgc gacaattata
     2041 gagatcgaac tacaagagag ccttgcggat atggctaagc gttcgattaa gctcaataaa
     2101 ttggaaaaac aggttactat gattaatgat gatttaaaaa atcttcttga tcatgttcaa
     2161 cgttcaaacg ttgatttaat gctttgcaat ccaccttatt tcaaagcaag cgaaacctct
     2221 aaaaaaaatc taagcccgca ttatctcctt gcaagacacg aaataacaac aaaccttaga
     2281 gagatttgtc aaattgctca acatgctcta aaaacaaaag gacgtatcgc tatggtacat
     2341 cgtccagacc gtttccttga aattattgat acgatgcgtc agttcaattt agcacctaaa
     2401 cgtatccaat ttgtttatcc aaaattagga aaagatgcta atatgctttt aattgaagct
     2461 attaaagatg gctcgacaga aggtatgaaa atacttcctc ctcttgttgt tcatcaagat
     2521 aatggagact atactgagac tatttttgat atatatttcg gagaaaacgg aaaatctcat
     2581 gattaaagtt cctgcttata tgtatgtttt agaatgttca gatggaacac tttatacagg
     2641 ttatactaca gatgtcaagc gccgtctcaa tacacacaat actggaaaag gtgctaaata
     2701 tactcgtgcg agattgccag taaaacttct ttattctgaa gcttttaata gcaaacaaga
     2761 agcaatgagg gcagaagcat tatttaaaca aaagactcgg caagcaaaat tgacttatat
     2821 taaacaacat aaaaatgaac aataatacga taatatacta agtgtaatga gcctatcctc
     2881 gcttctttaa cttttaaatt tcaacctcac tttaatcttt tgtcttcatt cttaaagtta
     2941 taaggcaatt ttatggatat cactatctac gctttatctt acaaaaaata aaaaaggagt
     3001 cgctcagcaa ctcctttttc ataatcctct tatttatcgc ctttgttgcg aataacaaat
     3061 ccagtttgtt tttcacttga agtgttttta tgaggttttt tattttcttg acgacgatta
     3121 tctttcttga acttatcatc acgacgttta aaatcacaac gaccaccacg atcatcacgg
     3181 tcacgatttc cacgacgatc accacgatca ttgcgtcgac gatcgccacc acggccattg
     3241 cctcgtccac ctttaccttt aaagccacca cctgatggtt taaatggtaa tggtttttca
     3301 cgtgtgattt caacttctgg taacgattct ggatcctgaa ctgttaagct taagacataa
     3361 agagcaagtt cttctggtgt gtattgagtt gctaattcta aagcatcggc tttaaattta
     3421 tcaaatttag aaactaattc ctgatcctca aagtcacgtg caatacgttt caatgcaact
     3481 tttttcttag cctgaaatgc ttcgcttgca gtagcaggct tcattccagt catacgtttt
     3541 ttagttaagt tttcaataat tgtcaaatat cccatttcgt ttggtgaaac aaaagtgata
     3601 gattgaccag atttaccagc acgaccagta cgtccaatac ggtgaacata actttcggga
     3661 tcttggggaa tatcatagtt gtaaacatgt gtaacgcctg aaatatctaa accacgcgca
     3721 gcaacatctg tggcaacaag aatatcaata tggtcatttt taaagtctct gataacacga
     3781 agacgtttat tttgatctaa gtctccatga attccttctg ctcggaaacc acgtaatttt
     3841 agtccacgag ttaattcatc tacacgacgt ttcgtacgtc caaagacaat tgacaattct
     3901 ggttgatcaa cgtccatcaa acgtgtcata gtatcgaatt tttcattttc cttaacacga
     3961 acataatact gatcaacatt cacattagtt aattcagttg ctttaatttt aacatgctca
     4021 ggatctttca tgaatttaac cccgatacgt ttgattggat caggcattgt cgctgagaat
     4081 aaaagagttt gacgtgtttc tggtacacga ctaatgattg cttcaatgtc ctctaaaaat
     4141 cccatgttaa gcatttcgtc tgcttcatca agaataagag tttcaatgtg attaagttta
     4201 agtgctttac gtttaattaa atcaagtaat cgacctggag ttccaacaac aacgtgtgct
     4261 cctgaacgta atgccttgat ttgtttttca atacttgacc caccataaac agagcggacc
     4321 ttaacacctt tatcgcgtcc aaaacggaac aattcttctt gtgattgtac tgcaagttca
     4381 cgtgtaggag caataataag tgcttggata gtattatctt cagtatgaat tttatttaaa
     4441 gttggtaacc caaaagctgc agtttttcct gtacctgtct gcgcttgacc aatgacatct
     4501 ttgccttcaa gagcaagtgg gatagtcatt tcttggattg gtgatggctc tacaaagcct
     4561 gctttttcta ccgctgaaag aatatcttgc gataagttaa gttctgtaaa tttcaaatgt
     4621 ttctcttttc taaaagacag tgcgaagctg ccttatgaac ctttattagt tatcttttct
     4681 gacaacctac ttagtgtaac acaattaaaa gtaaaaagat ataaaataag gtcaatctaa
     4741 taaataacac gctttcataa attgctagtt ctattagttg cttcagaaaa tagaaaaaag
     4801 gcaataaaaa cccaccatct ataaagaagg agggttcata gtttatctat ctagaacgca
     4861 acggctgctt cacttggaat ctccttagtg atatagtgac gaatctcttt acttgttagt
     4921 acttctttaa gtacttttat tttatcacta tttttattat cttcacgagc taccaaggca
     4981 cctgcaaaac ggatatctgg ttttttctca tataataatc tatccctttt aggaactaga
     5041 ttgatttttg tcacatatcc agggaaatta aagacaaggt catattcaaa ataggcctga
     5101 ttgaggttaa gtaatgctac aggttcaatt cgtaattttt tagggttagt aataatatct
     5161 tcgatagcct tggtcttttt ggacgtattc tttaaggtga taagtttctt ttcttgcaat
     5221 aatagcagag ctctagtcat attggcagga tcagacggaa tcgctacctt agcaccgtct
     5281 ttaagctcgg caatattttt caaatgttga ccataaaaac cagccaatga atgataaatt
     5341 ggtgtaattg atactagatg accatcattt tccttattaa agatactcat gaaaaattca
     5401 tgttgtaaaa ggttagcatc atgttctttg ttttctaaag caatgttagc ctgaatatag
     5461 tcagagacca tgacaacctt taaagtatat cctttttctt ttacattatc tttaaccaaa
     5521 tctaaaaagg tagatgtagg tttgctgtaa gttgcaacag taatcacctt ttctttacta
     5581 ttcttatctt taattttctt agacgacatt gcttgcttag tacatgccac taaacataaa
     5641 gacatcatga ttagacatgt tagggaaagt aattttttca tagttcctct tttcttttat
     5701 tttcttgagt agcggtttgc tataaaatag cccaaactct gtaagataaa cacatatatt
     5761 ataaataaga caacaatcaa gtacataact gggtagtcat actcttgata gccaaaacga
     5821 taggcatatt cacccaaacc tcctgcacca ataactccca ttattgtcga gtagccaaga
     5881 atacttatag ccgttgcagt aaatgataaa acaaggtcaa tcttgatgga ggggattaaa
     5941 aaataataaa ttatctgtct cttgttagcc cctagactga gagctcgatc tacaacaact
     6001 tgaggtatat ttaataaagc ttgttccaca taacgtgcat acaaactgac tgaaaccaaa
     6061 gtaagaggta agattgccgc tatcgttcca aatgaggtcc caaaaattaa tcgattgaga
     6121 gggattaaga taaaaataaa aataaggaaa gggacactcc gtaacgtacc taaaaataaa
     6181 tttagtagtt gataggctaa agaatgttta atcaagtaag actttctcaa tgaaaacaat
     6241 aaaatacctg ttggaaaagc aattagaaaa caaagaatca aggttaaccc taacattaaa
     6301 ttggtttccc aaaatgattt taagatacct tcgccatgtt tttcaataat gtcaatcatg
     6361 taacaaaaac tcctttactt gatctacgta gtttgacttt aattgattca gagttgcttt
     6421 cactggaaca acagtatcta caatagtctg gtgatccaaa atcattacac gatcacaata
     6481 ttgttttaaa agagataggt gatgagcaat catcaagata gagataccat atttttcacg
     6541 aagatcagac aacaagttaa atatctcttt ttcactatta gtatctaagg cagagctgat
     6601 ctcatcacat agaataagtt tcggtttttg caaaacagcc atagcgattg caactttttg
     6661 ttgctgccca ccactcaaac tatggcattt cacctgtttt aaatccatca aacctaaaaa
     6721 atctaagata tcattaattg cgtcatgatt tactttttga tgatttaatt tataaactaa
     6781 agctagatga taatatacac tttctcctgc taacaaatta tgattttgaa aaatataagc
     6841 aatatcttta cgcatctgac gtaacttatt atcagaaaaa gacttcactt cttttccttg
     6901 ataataaata tttcctttat cataagggat taagccatta atcatcttca aaatagtact
     6961 ctttccagta ccattagaac caatcagacc aactatctcg ccttcttcta tattaaaaga
     7021 aatatcatct aatctaaact gtgattttgg
//
LOCUS       DATRNK010000076         6905 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.076, whole genome shotgun sequence.
ACCESSION   DATRNK010000076 DATRNK010000000
VERSION     DATRNK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.076"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            285..1400
                     /locus_tag="VBC52_001782"
     CDS             285..1400
                     /locus_tag="VBC52_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO6883344.1"
                     /translation="MSDDLISIQSSCLSIAKTFANFNIQRKRFMISTETEMDTFHKKD
                     DIDVWVGGKSYNPARSFRTRTINELTVIDFEEMFDILWLMLGDNLIKSFEVNVCGILF
                     ELGGNDIPSTFRQENIDPLINKWWYDNVSTEIIPNLIKKLKENPLFNIGFMVNDILER
                     MYKENIPKSYLTSVPLVISQKGRTTYSFSMTGGQQIDGVKFKQIYEDYMKLLSQGKDI
                     AELYQKYSKEELANLGINIYQSNDIERTEERTFDEIISWVSNPYATRPIQERHTIQLE
                     PTRFSLEDKKRIEEAAAQGLSEIDLIDLVDLYDINLDNTSVNRHIVGLLTNNTQVTYY
                     FQEQLNKELLSMAHALDNVQQAFIKLLSEEEIRKFAL"
     gene            1721..2170
                     /locus_tag="VBC52_001783"
     CDS             1721..2170
                     /locus_tag="VBC52_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6883345.1"
                     /translation="MSNKVQERRERKIKEAIKAKNWDEVTRLLQQEQSNAERRDRYHN
                     RRIKDETIASKNAKKSVRYDVIASSDLNPEEALILEELRQAIREAKASLSEIDSKIVE
                     MIAEQGSSYKETARYITEHYKKMSDVTVKSHYCKALKKLAPLLKAYR"
     gene            2373..2693
                     /locus_tag="VBC52_001784"
     CDS             2373..2693
                     /locus_tag="VBC52_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883346.1"
                     /translation="MAFKMKTTKGGYTTTLADKIISQKQPIYSLSTELEPQQRFEEGK
                     PTGEIVAYKAWFVQEGQDPFQVKFEETIELPAFQSMIQFDTLQACEVKYNIYFKANGI
                     KEVR"
     gene            2693..3793
                     /locus_tag="VBC52_001785"
     CDS             2693..3793
                     /locus_tag="VBC52_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO6883347.1"
                     /translation="MTSQSRDVLNQSYLQSYLLQSLNMALGALMQGETSYTNSFNIVI
                     QADGFIFVPRLPCAYILDDDLYKKIFLIANASLYPQYTLLKQNATDFVPLETDDLHTQ
                     RGLFFPWKKGISERLVIPDLDKFSASLPQGKIPIMKYFELNLDKVNHWAIAGNSGSGK
                     SYALTYFLSVLKHMSDLIIIDPKFDTPSRWARENHVAVIHPVENRSKSDFVSQVNEQL
                     SQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQISLLG
                     RATKIHLFLVSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPIG
                     HGTGIIQVIDNEHPYQVLPLLCPTYYTKRGIL"
     gene            3790..4476
                     /locus_tag="VBC52_001786"
     CDS             3790..4476
                     /locus_tag="VBC52_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883348.1"
                     /translation="MTNKTYFMRSFNLILYLFLFAPICSAFGWLFNYLSVQLTNITFV
                     NLDTVKSITDIISSVCHGFALISLLLALFLVGLEIIQRLKTDSLWNYIQSVYHTFLLR
                     HFLFQRERVQKISNLEHQTVTTINPIHNGFNRAVRKCIVDIRKDTITIFLKVPRDQQG
                     QKILKEMEAQLKEEIVSQHADYYFSSPIRVRNQLWFTEQKLNPLGLWLALSKSQTAPF
                     YILFYHDLLG"
     gene            4600..5205
                     /locus_tag="VBC52_001787"
     CDS             4600..5205
                     /locus_tag="VBC52_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEO6883349.1"
                     /translation="MAKEQRSTKWTFLFYEESAPENYLNILEELHIPFILSPWHDKDV
                     NRQTGEFKKSHKHGAFFFDSLKSYSQVSNIISDKLNGPAHVEVVMSPTGLFDYFTHAE
                     NPDKTPYNIEDIEVGCGFNLEKFLMEMNSSDFIHEVVDIIEENDFTEFEELVWYARAN
                     NTNLLGLIIERTYFFAKYLDSRRYNPNRLHNSNTEEKENNE"
     gene            5198..5446
                     /locus_tag="VBC52_001788"
     CDS             5198..5446
                     /locus_tag="VBC52_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6883350.1"
                     /translation="MNNEKLEQIEQLLQTLIKLIQIKEQNIPLKIIQQRELLKQLNIS
                     PNTLKTWEQKGLKRLEPPIEGTRTVFYLLDDIINFLQS"
     gene            5536..6794
                     /locus_tag="VBC52_001789"
                     /pseudo
     CDS             5536..6794
                     /locus_tag="VBC52_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612645.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         2455 a         1257 c         1173 g         2020 t
ORIGIN      
        1 aaaattattc acaagaaata cctactcgta tcaaaaattt aagaaaaaaa tacaatatta
       61 cacaaagcga actaggcaat gcaggtcaag ttagtcaagt tgaaagtggt aagcgaccaa
      121 ttacgagttc gatgttagtt tatttaaatg ctctaaccgc ttcgagctat acgtatatcg
      181 tttttggaga gttagatgag tttattgaga atctgtttca ttattttttc agttctattt
      241 tatatagaga tttagaggct gtagatgaaa aactatattc tttcatgagt gatgatttaa
      301 tttcaatcca atcaagttgt ctaagtattg ccaagacatt tgcaaatttc aatattcaaa
      361 gaaaaagatt tatgatttct actgaaactg aaatggatac ttttcacaag aaagatgaca
      421 ttgacgtatg ggttggtgga aaaagttata accccgctag aagttttaga acccgtacca
      481 tcaatgaatt gactgtcatt gattttgaag aaatgtttga tattctttgg ttgatgttag
      541 gggataattt aattaaatca tttgaagtta atgtctgtgg tatcctattt gaattaggcg
      601 gaaatgacat accctcaaca tttaggcaag aaaacattga tcctctcatc aataaatggt
      661 ggtatgataa tgtttcgaca gaaattatcc ctaatctaat aaaaaaactc aaagaaaatc
      721 ccttgtttaa tatcggattt atggtaaatg atatattaga aagaatgtat aaagagaata
      781 ttccaaaatc ttaccttaca tccgtcccat tagttatttc tcaaaaagga agaacaacct
      841 attcgtttag tatgactggt ggtcaacaaa tagatggagt gaaattcaaa cagatatatg
      901 aggactatat gaaattactc agtcaaggta aggatatcgc agagttatat caaaaatatt
      961 ctaaagaaga gttggcaaat ctaggcatta atatttatca atccaatgat atagaaagga
     1021 ctgaggaaag aacttttgat gaaattatca gttgggtttc caacccttat gcaacaagac
     1081 caattcaaga aaggcacact attcaattag agccaacaag attttcacta gaggataaga
     1141 aaagaattga agaagctgca gctcaaggac taagcgaaat cgaccttatt gatttagttg
     1201 acctatatga tattaattta gacaatacaa gcgtcaatcg ccatattgtg gggttattga
     1261 ctaataacac ccaagtaaca tactatttcc aagaacaatt aaataaggag ttgctgtcaa
     1321 tggctcacgc tttagataac gtacaacagg cctttattaa attattaagt gaagaggaga
     1381 tacgaaaatt tgctctttaa agactaggtg aatcgcctag tctttttttg cgtctcgttt
     1441 tacaaattag tctattgaag taaaaaaaga agtaaaaaat aaaagaacat tactccttat
     1501 actgaatata tcaagaaaaa ctcttgatat aaattttaat agtccaaatt gatggccatc
     1561 aaaggacaga ttgaaacaaa tatgatgaca tgacgataaa ggtcatgtct tgcatagaat
     1621 tgtttcgctg tttctttgtt gtgccttttg gtatactcag cgttcaatat ttgctcattt
     1681 tgtttccttc tgtccaaagg acagaaagga gctcatcatt atgagtaata aagtacaaga
     1741 acgtcgtgag cgtaaaatca aagaagctat taaagctaaa aattgggatg aagtaactcg
     1801 tttgcttcaa caagaacaaa gtaacgcaga acgtcgtgac cgataccaca atagacgaat
     1861 caaagacgaa acaatagcta gcaaaaatgc caaaaagtct gtacgttatg atgtcattgc
     1921 aagttctgat ttgaatcccg aagaagcttt aatcctagag gagttaagac aagctattcg
     1981 tgaagctaaa gcatccctat cagaaattga cagtaagatt gttgaaatga tagcggaaca
     2041 aggttcaagc tataaagaaa cggctcgcta tatcactgaa cattataaga aaatgagtga
     2101 tgtaactgtt aaatcccatt attgtaaagc gctaaaaaaa ttagctcctt tattaaaagc
     2161 ttaccgctaa ttcctcaaat ccagtgtctc tgtcactgga ttttttgtaa aaaaaaaatt
     2221 aaaaattcaa caaataaatt tgcgtttagc tgtctcattc tccttagtaa tgaggaggtg
     2281 gtcagataac ctaaaaaaca aagtccagct catgacccaa accgagcctg tcgcattcac
     2341 actatctctt attcataagg aggatttgcc ctatggcgtt taaaatgaaa accacaaaag
     2401 gtggttatac aactacattg gctgataaaa ttatcagtca aaaacaaccg atttactcac
     2461 ttagtaccga acttgaacca caacaacggt ttgaagaagg aaaaccaacc ggagaaatcg
     2521 tagcttataa agcatggttt gtacaggaag ggcaagaccc tttccaagta aaatttgaag
     2581 aaacgattga gcttccagct tttcaatcca tgattcaatt tgacacacta caagcctgcg
     2641 aagtaaaata taacatctac ttcaaagcaa atggtatcaa ggaggttcgg taatgacttc
     2701 acaatctcgt gatgtgctaa accaatctta cctgcaatct tacctgctcc agagcctaaa
     2761 catggctctg ggggctttaa tgcagggaga aaccagctac accaattctt ttaatattgt
     2821 tattcaagca gacggcttta tctttgtccc tcgtctacca tgtgcttata tccttgatga
     2881 tgacttgtac aagaaaatct ttctgattgc caatgcttca ctttatccac aatatacgct
     2941 tctaaaacaa aacgctaccg attttgtccc tctagaaaca gatgacttac acactcagcg
     3001 tggattattt ttcccttgga aaaagggaat ttcagaacgt ttagtcattc cagatttgga
     3061 taagttttca gctagcttgc cacaggggaa aatccctatc atgaagtact ttgaactcaa
     3121 tttagataag gttaatcact gggctattgc tggaaattca ggttctggga aatcgtacgc
     3181 tctcacttat ttcttgagtg tactaaagca tatgtctgac ttgattatca ttgaccctaa
     3241 atttgatacc ccaagtcgat gggcaagaga aaatcacgtt gctgttatac accctgttga
     3301 aaaccgttca aaatcagatt ttgtctcaca ggtcaatgaa caattaagtc agtgtgcaac
     3361 tctcattcag aaacgacaag ctatcttgta tgataatccc aatcatcaat ttacccatct
     3421 aactattgtt attgatgaag tcctcgctct atcagaggga gtcaataaga atatcaaaga
     3481 agcctttttc tccctactct cacagatttc cttattagga cgtgctacca aaatccatct
     3541 gtttttggta agccagcgtt ttgaccacaa tactattcca atttcagtga gggaacagct
     3601 aaacgtattg ttgcaaattg gaaatatcaa tcagaaaact actcaatttt tatttccaga
     3661 cctagaccca gagggtattg ttattccaat aggacatggg acaggtatca ttcaagttat
     3721 cgataatgaa catccctacc aagttctgcc cttactctgt ccaacctact acactaaacg
     3781 aggtattcta tgaccaataa aacctacttt atgagaagtt ttaatctcat actatattta
     3841 tttctatttg cacctatctg ttctgcattt ggatggttat tcaattatct ttccgttcaa
     3901 ttaacaaaca tcacgtttgt caatttagat acggtcaaaa gtattacgga tatcattagt
     3961 tccgtttgtc atggatttgc acttatttct cttctacttg ccttattttt ggttggatta
     4021 gaaataatac aacggttgaa aacagatagt ctttggaact atatccaatc tgtctatcac
     4081 acttttctgt tgcgacactt cctgtttcag cgtgaacgag tacagaagat aagcaatctt
     4141 gaacatcaga cggttactac tatcaaccca atccataacg gatttaaccg agctgttcgt
     4201 aagtgtatcg tagatatacg aaaagatacc atcactattt ttctaaaagt ccctagagac
     4261 caacaagggc aaaaaatcct aaaagagatg gaagcacaat taaaagaaga aatcgtcagc
     4321 caacacgcag actattattt ctcctctcct attcgagttc gtaaccaatt gtggttcact
     4381 gaacaaaaac ttaatccctt ggggctgtgg cttgcgttaa gcaaaagcca gacagcccct
     4441 ttttatattc ttttctatca tgacctattg gggtgactac gacccccaat aggtcaataa
     4501 tcaataatca caaaaaattc aattttttca aaaaaacttt gcatacgacc tgtcatttct
     4561 ccttagtaat agaacccaaa agaaatgagg taactgccta tggcaaaaga acagcgctca
     4621 accaaatgga cttttctctt ctacgaggaa agcgccccag agaactactt gaatatatta
     4681 gaggaactcc atattccgtt tattcttagt ccttggcacg ataaggacgt taatagacag
     4741 acgggagagt tcaagaaatc gcacaaacac ggtgctttct tctttgattc actgaaaagc
     4801 tattcacaag tatcaaatat catcagtgac aaactaaacg gtccagcaca tgtggaggta
     4861 gttatgtctc ccactggttt atttgactac tttacccatg cggaaaatcc agataagacc
     4921 ccctacaaca ttgaggatat tgaagtcggt tgcggcttta atttagaaaa attcttgatg
     4981 gagatgaact cctcggattt tatacatgaa gtagttgata ttatcgaaga gaacgacttt
     5041 accgaatttg aagaattggt ctggtatgcc cgagcaaaca ataccaattt attagggctt
     5101 atcattgaac gtacatattt cttcgccaaa tacctagatt cacgaagata caatcctaat
     5161 cgactccata attcaaacac agaggagaaa gagaataatg aataacgaaa aattagaaca
     5221 aattgaacaa ttattgcaaa cacttatcaa actcattcaa atcaaagaac agaacatacc
     5281 actaaagata atacaacagc gagaattgct aaaacagcta aatatctccc caaacacact
     5341 aaaaacttgg gaacaaaaag gattaaaaag attagaacct cctatagagg gaactcgaac
     5401 cgtcttctat ctactagatg atattatcaa ctttttacaa tcctaattct gaaaatttgt
     5461 gctaaaatag ttttatctaa aagaaagagt ggtaactatt ttagcctact tcaatatcca
     5521 tgaagaaagg caaatatgga aactgaaaca attattcata atggcaaaaa agtcattaaa
     5581 aaaatcaaaa aagataagtc catctcatac actttgaagg ggacatttct gggaaaagat
     5641 atcaaaactg gtaagcaagt cactactacc attacagcca agacactaaa acagttagat
     5701 agagcaatca ttcaagcacg actagaattt gaaaaaatgg ctcaacacga gaaaaagtta
     5761 tcgtcattga tacactggaa gatttagctg aagaatggtt taaatcatat aaaacctggg
     5821 ttaactcgca taatactctg aacagagtaa gaagttatct tgataattat attatcccta
     5881 aatttggaga ttacaaacca gataaaattg aatctgcgga tatccagctt tggttaaacg
     5941 atttggcaaa gaaatcaaaa gagtccattg aatctggtat aaagcgtgct gataaaggta
     6001 gtgctaaaga ttttggtgct gttatccata agttgaaaga tatttttgac tatggaatta
     6061 caaactatgg actaatcact aatcctgtta gcactgttaa gataccacct aagccaaaat
     6121 caaataagca acgcatcatg gtattacatg atgatggtct tgtaatatgg cttaattatc
     6181 ttgaaagttt agataacaat agagccaatc gtagatttaa gttaatttgt aatactctat
     6241 tagcctccgc tttgcgaata aatgaattac ttgcattgac gattgatgac ctagattttg
     6301 aaaattcttc tattaacgtc agtaaaacat tgatgtggaa aaccgcaaat aaaaaagatg
     6361 ggacaaaagg ggaagttatc tgtaaagcta caccaaaaac tgatgcagga aatcgctctg
     6421 tccctccccc cataatagaa aaactcagag atttccacca cgaaatgaac aagtatcttg
     6481 aattgcacaa cagacataga acaaaactga ttttccctac aatctatggg aactatatgt
     6541 gtgacagaaa cgagagaaca actcttaaga aaaggttagt aggtcttgga ttaccaaact
     6601 atggtttcca tttattccga catactcatg cttcaatgct acttaatgct ggaacaaatt
     6661 ggaaagaact ccaagtcaga atgggacata aatccatttc aactacaatg gatacttacg
     6721 ctgaattagc accacaacga aagttagaag ctgtggggat ttatcttgaa aagattgctg
     6781 agctaacgca ataactactc taccattcac tctacctttt attatgtagc attctagaaa
     6841 cattgatgta ccaacgtttc taacagctaa aatagaaatt atttaactct cactgaagtt
     6901 aaata
//
LOCUS       DATRNK010000077         6817 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.077, whole genome shotgun sequence.
ACCESSION   DATRNK010000077 DATRNK010000000
VERSION     DATRNK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.077"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            141..638
                     /gene="ybeY"
                     /locus_tag="VBC52_001790"
     CDS             141..638
                     /gene="ybeY"
                     /locus_tag="VBC52_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEO6883351.1"
                     /translation="MYVEMIDETGQVSEDIKKQTLDLLEFAAQKTGKENKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPEVDISFDEEDLTENPELAEMLEDFDSYIGEL
                     FISIDKAKEQAEEYGHSYEREMGFLAVHGFLHINGYDHYTPEEEKEMFSLQEEILTAY
                     GLKRQ"
     gene            619..1017
                     /locus_tag="VBC52_001791"
     CDS             619..1017
                     /locus_tag="VBC52_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEO6883352.1"
                     /translation="MDLNDNNQKKWKNRTLTSSMEFAVTGIFTAFKEERNMRKHLVSA
                     ILVILAGLTFQVSMVEWLFLLLSIFLVITFEIINSAIENVVDLASNYHFSMLAKNAKD
                     MAAGAVLVVSLFAVLVGLIIFIPKILALLL"
     gene            1059..1958
                     /gene="era"
                     /locus_tag="VBC52_001792"
     CDS             1059..1958
                     /gene="era"
                     /locus_tag="VBC52_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEO6883353.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGD
                     DMIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALQGNNVST
                     LIKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
     gene            2195..2584
                     /locus_tag="VBC52_001793"
     CDS             2195..2584
                     /locus_tag="VBC52_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883354.1"
                     /translation="MHNILRFLGIVIISAMILLSIGSFYDLSLMKNILLICWSFLLDL
                     LVFVFKQRQTTEVLTWYQVVKQFWLFIKCTILIPFIIMIGCLTSISDISIYFYLHLVV
                     VYYTIGMILSLGHIISPEHSMFNKLRK"
     gene            3044..3235
                     /locus_tag="VBC52_001794"
                     /pseudo
     CDS             3044..3235
                     /locus_tag="VBC52_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973012.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3314..3570
                     /locus_tag="VBC52_001795"
                     /pseudo
     CDS             3314..3570
                     /locus_tag="VBC52_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            complement(3907..4101)
                     /locus_tag="VBC52_001796"
     CDS             complement(3907..4101)
                     /locus_tag="VBC52_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883355.1"
                     /translation="MIMSGQRATSTIQSDINIFHHIEAICISKSNNIGLPAIQKHLGQ
                     LIQREHLGPFFSITYSKAPT"
     gene            4100..4294
                     /locus_tag="VBC52_001797"
     CDS             4100..4294
                     /locus_tag="VBC52_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883356.1"
                     /translation="MLFLMIKPDYIRYFKLINDMRDTMYQQTVFHVQPQAWKYTMKSI
                     DQLVVDDLNHISNEKTTLVK"
     gene            complement(4345..4521)
                     /locus_tag="VBC52_001798"
     CDS             complement(4345..4521)
                     /locus_tag="VBC52_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883357.1"
                     /translation="MSKKTDSNYLHSNCSDKKIWSDEEPLQQETIPKDRDCVIKPVIT
                     MTVEHVEIPLDDQS"
     gene            complement(4557..6149)
                     /locus_tag="VBC52_001799"
     CDS             complement(4557..6149)
                     /locus_tag="VBC52_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HEO6883358.1"
                     /translation="MKIKKIISGLAAALIISSLSTINYEVKADDTTSEYHYISKQNNE
                     EQLISYIKEQHRLLNQFVVDNVNSFTQLNANPTIEQLNRAITLFKQKDEQLFNQVKAS
                     HLSPSNYNAIVNQRNVINQTVQNLIDQNHNKLQTSQNKAAQLVGQRNQVVNKIQAILA
                     TVNYNSVNSIQEAENLFHSLRNQIEPLVAEVNNYKAAMATLQQEVDALSTAAIETETS
                     KLATLKVSENTSVPANKVEEKTTQSEASGNKQEVTKSEEKQATSDAKASQPESANIAD
                     YDSLKEVLRNNISNQVPHISVQMEFKTQEQVDEYQKNLGSIIREIGDTLGTATEFNAK
                     SNISTYTLGGQIQRIIVKSDITITYTLKGDMVGLHKEYKQFVDSFVKENITNKNITSD
                     YEKAKVIHDHLVNNYTYATEELATTRETASGISIHAPEALYKDKRGVCQAFAVMFKDM
                     AAAAGLSVWYVTGQAGGGNHAWNIVTINGVKYYVDTTWDNNIKSNKYFLVGKTIMDAD
                     HLLDSQYNALAKDIPADRHQGA"
     gene            complement(6210..6278)
                     /gene="shp2"
                     /locus_tag="VBC52_001800"
     CDS             complement(6210..6278)
                     /gene="shp2"
                     /locus_tag="VBC52_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEO6883359.1"
                     /translation="MKKINKALLFTLIMDILIIVGG"
     gene            6366..>6817
                     /locus_tag="VBC52_001801"
     CDS             6366..>6817
                     /locus_tag="VBC52_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6883360.1"
                     /translation="MEKELGKTLRRLRKGKKVSISSLADEHLSKSQISRFERGESEIT
                     CSRLLNILDKLNITIDEFVSIHSKAHTHFFILLNRVRKYCAEKNVTKLVALLEDHNHK
                     DYEKIMIKALIFSIDQSIEPNQEELARLTDYLFTVEQWGYYEIILLGN"
BASE COUNT         2136 a         1019 c         1261 g         2401 t
ORIGIN      
        1 aggaagagaa cgcactttta ttttgagaac tagttttaga taaaagtgaa aaacttagga
       61 aagtagatgt gtcgattatt tagaattatg cattacactt tatcttttct aaacgttttg
      121 ggtcgttctt actatataag atgtacgttg aaatgattga tgaaactgga caagtttcag
      181 aagatatcaa aaagcaaact cttgatttgt tagagtttgc agcacaaaaa acaggtaaag
      241 agaataaaga aatggctgta acgtttgtca ctaatgaacg tagccatgaa ttgaatttag
      301 agtatcgaga taccgatcgt ccgactgatg ttatcagttt agaatataag cctgaggttg
      361 atatttcttt tgatgaagaa gatctgactg aaaatcctga acttgctgag atgctagaag
      421 attttgattc ttatattggt gaacttttta tttctattga taaagctaaa gaacaagcag
      481 aagagtacgg tcactcttat gaacgtgaaa tgggtttttt agctgtgcat ggttttttgc
      541 atattaatgg ttatgatcat tatacacccg aagaagaaaa agagatgttc agcttacagg
      601 aagaaatttt aactgcttat ggacttaaac gacaataatc aaaaaaaatg gaaaaatcgc
      661 accttaacat caagcatgga atttgctgtt acagggattt ttacagcttt taaagaagaa
      721 agaaatatgc gtaagcattt ggtttctgcc attttagtaa tacttgctgg tcttactttt
      781 caagtatcta tggtggagtg gttatttctt ttattgagta tttttttggt aattactttt
      841 gaaattatca attcagctat tgaaaatgta gttgatttgg caagtaatta ccatttctcg
      901 atgctggcta aaaatgctaa agatatggca gcaggagcgg ttttagttgt ttccttattt
      961 gccgtattgg tgggattgat tatttttata cccaagatac ttgctttact tttatgatga
     1021 gaagtattac gaataaattg aatggagaaa gaaaagaaat gacatttaag tcaggatttg
     1081 tagcaatttt aggtcgtcca aatgttggaa aatcaacctt cctaaaccat gttatgggac
     1141 aaaaaattgc tatcatgagt gataaagctc aaacaacacg taataaaatt atggggattt
     1201 atacaacaga aactgagcaa attgttttca ttgatacccc aggaattcat aaaccaaaga
     1261 ccgcccttgg tgattttatg gttgaatctg cttatagtac tttacgtgaa gttgaaacgg
     1321 ttcttttcat ggttcctgcc gatgaaaagc gaggtaaagg cgatgatatg attattgaac
     1381 gtcttaaggc tgccaagatt cctgttattt tagtaatcaa taaaattgat aaggttcatc
     1441 cagaccaact tttggaacag attgatgact tccgtagcca aatggatttt aaagaagtgg
     1501 tgccaatttc tgcccttcaa ggaaataatg tgtcaaccct aatcaagtta ttaactgata
     1561 atcttgaaga aggtttccaa tatttcccag aagatcagat tactgatcat ccggaacgat
     1621 ttttagtatc agaaatggtt cgtgagaagg tacttcattt aacgcaacaa gaggttcctc
     1681 actcagtagc tgttgttgtc gaatccatga aacgtgatga agaaactgat aaagtgcaca
     1741 ttcgtgcaac tatcatggtt gaacgtgata gtcaaaaagg cattatcatt ggaaaacaag
     1801 gtgctatgct caagaaaata ggaaaaatgg cgcgccgtga tatcgaactt atgttaggtg
     1861 ataaagttta cctagagact tgggtaaaag tcaagaaaaa ctggcgtgat aaaaagcttg
     1921 atttagctga ttttggctac aatgaaaaag agtattaatg agtttatttt gagattcgca
     1981 agttagtggg gaagagttag ataagttcac cgtgagactt gattcgcaca ggaaaagcaa
     2041 ttcacgagga caaaaccaac tagtcataac tttatgctta tattcacaaa tcaatcaaag
     2101 ggggccaaac atgcttgttg gctccttctt aaataaagga gagcacatga ctcaagatta
     2161 tatttgctac cgataaagta gaggaggtga ggatatgcat aatatattaa gatttttagg
     2221 cattgttatt atttctgcaa tgattctact tagtataggt agtttctacg atttatcatt
     2281 aatgaaaaat atactcctta tctgttggag ttttctcctt gatctattgg tttttgtctt
     2341 caaacaaaga caaacgacag aagtgctcac atggtatcaa gttgttaagc aattttggtt
     2401 atttattaag tgtactattc ttattccttt tattataatg ataggatgtt tgacaagtat
     2461 atcagatata tcaatttact tttatttaca tcttgtcgtt gtttattata cgataggaat
     2521 gatcttatca ttaggtcata tcatttctcc tgaacactct atgtttaata aacttagaaa
     2581 gtgaaatgaa ttatatttaa aatttgtttt caacagagcg gatttggcca tctgtcgttt
     2641 gccatgcctt tcataatttt tcagtaatta tgatagttgt tctgttctcc caatcataaa
     2701 actttaaata agattctatt agttcttgcc tcactctcct taagtctctg gtaaaaaccg
     2761 aaaagttacc agactttaaa ggggagtttt ttatctgtca acagttagta gaagaacgat
     2821 atattgctcc atttagatta attatggaga taaccaaata atagcttgtg ataataagat
     2881 acctttgaag tttcagaaac aatctgtttt ccattctgct atattcatct ttgccaaagt
     2941 tgcaacaaca caataataac aattgcttgg gcattgggtg attagtttac tatcaatagg
     3001 gttagagtgg agttggagta tatggattgg cattaatctt ataatgatga ttctgttgag
     3061 ttatgtgcta acaaaaactg agccaatcag taactaccaa gagtaatcat ctaagctcaa
     3121 aaaatggcta tttgcaatcg gtgcttttag cgttttattt tgctgggcac tactagtaaa
     3181 aacatttttc ccaataaagc aaaaccagaa gggattaaca gctgatatga attaggtcaa
     3241 tcactacttt cgagttgtct tattattgac tatctgtctt attgccccaa tttaagaaga
     3301 gataacgttc aggttgtttg tcatgagaac atttgggaaa actccctgag tggtcccttg
     3361 gttgtgatct tatcttctat actatttagc tggtctcact tacatggtct atctgtcgtc
     3421 gactttgttg tctattttgg tatgggtttg attttcgcta gtttgcacca ctatacaaaa
     3481 tccattcatt attcgattgg cgagcatatt atttggaata gtttgctata tattttttat
     3541 tttctagcat ttttgcttga tttactctaa gtaataagat tgagagacta atcattacta
     3601 agacattttt gtcagtttaa aagagctttt tggctctttt gttatttgtt tttttaatga
     3661 ataaaatgtt attattttat tgttaactaa ataaagttcg taattttata tgatttatat
     3721 agaattacga gttttttgtt agactaatat gagaaaatat agtatgacga attcctaaat
     3781 aaaaaataat gaaaggaaca tgatgttaaa agatgttaac gttggaatga gtcatttatt
     3841 agttagaggt gctattattc tagtagtagg gattgttttt ctgttgcaac tgattcaata
     3901 agtacttcaa gtgggagctt tggaataggt tatcgagaag aaaggaccca gatgttctct
     3961 ttggatcagc tgtcccaaat gcttttgtat tgcggggagc ccaatattgt tagatttact
     4021 tatacaaata gcttctatat gatgaaagat atttatatcg ctctggatgg tgctggtagc
     4081 tctttgaccg ctcatgatca tgctattcct aatgattaaa ccagattata taaggtattt
     4141 caaactcatt aacgatatgc gtgataccat gtaccaacag actgtttttc atgttcaacc
     4201 gcaagcttgg aaatacacca tgaaatcgat tgatcaatta gttgtagatg atttgaatca
     4261 tatcagtaat gagaaaacca ctttggttaa ataaaaaaga acactgtcaa aaatggcagt
     4321 gttccttttt attttaggag agagctaaga ttggtcatct aatgggattt ccacatgttc
     4381 aactgtcata gtaatgacag gtttgataac acaatctcta tctttaggga tagtctcctg
     4441 ttgcagaggc tcctcatcgc tccaaatttt cttgtcagaa caattggaat gtagataatt
     4501 tgaatcagtc tttttagaca tttcatatcc tttccaggct caatacaatt tttgagttat
     4561 gcaccttgat ggcgatcagc tggaatatct ttagctaatg cattgtattg actatccaaa
     4621 agatgatcag catccattat tgttttacca acaaggaaat atttattgct ttttatatta
     4681 ttatcccatg ttgtatcaac ataatattta acgccattaa tagtaacaat gttccaagcg
     4741 tgatttccac ctccagcttg accagttaca taccatactg ataagccagc agcagcagcc
     4801 atatctttaa acattactgc aaaggcttga caaacaccac gtttatcttt gtagagtgct
     4861 tcaggagcat ggatactgat accactagca gtttcacgag tggttgccag ttcttcagtc
     4921 gcgtaagtgt aattattaac caagtggtca tgaattactt tagctttttc ataatcactt
     4981 gtgatatttt tgttagtaat attttctttg acaaaagaat ctacaaactg tttatattct
     5041 ttatgtaatc ctaccatgtc acctttaaga gtataggtga ttgtgatatc gctttttaca
     5101 ataatgcgtt ggatttgtcc accaagagta taagtgctaa tgttactttt ggcattgaat
     5161 tcagttgctg ttccaagtgt atctccaatt tcccggatga tgcttccaag atttttttgg
     5221 tattcgtcaa cttgttcttg agttttaaac tccatttgaa cactgatgtg tggtacttgg
     5281 ttactaatat tatttcgtaa aacttctttt aaactatcgt aatcggcaat attagctgac
     5341 tcaggctgtg atgcctttgc atcagaggta gcctgttttt cctcactctt agttacttct
     5401 tgtttattac ctgacgcttc tgattgagta gttttttctt ctactttgtt tgcaggaaca
     5461 gaagtatttt cgctaacttt gagagtagca agtttagaag tctcagtttc aatagccgct
     5521 gttgataggg catctacttc ttgttgaagg gttgccatag cagctttgta attattaact
     5581 tcagctacaa gaggttcaat ttgatttctg agtgaatgaa ataaattttc agcttcttgt
     5641 atagaattca cagagttgta gtttacagtt gctaaaatag cttgaatttt gttaacaacc
     5701 tgattacgtt gccccacgag ctgagctgct ttattttgac ttgtctgaag cttattatga
     5761 ttttggtcaa tcagattttg aacagtttgg ttaatgacat tacgttgatt aacgatagca
     5821 ttatagttac tgggagagag atgactagct ttcacctggt taaataattg ctcatctttt
     5881 tgtttaaata atgttatagc tctatttaac tgttcaatgg ttggattagc atttagttga
     5941 gtgaatgagt tgacattatc aacaacaaat tgattgagca aacgatgttg ttccttgatg
     6001 taactaataa gctgctcttc attattttgc ttactgatat agtgatactc actggtggtg
     6061 tcatcagctt taacctcata gttaatagtt gatagtgaac tgataattaa agctgcggca
     6121 aggccactaa taattttttt aatcttcatt agttctcctg ttataaacta aataaaatgg
     6181 gattccataa caaccatttg tggggagttt tatccaccaa cgataattag gatatccatg
     6241 attaatgtga acaacaaagc tttgttaatt tttttcatga ctatctcctt tctgattttc
     6301 tattttgtac ttgtattata taatggattt aaagggtctt ttaaattgtt gtgaaagtgg
     6361 gaaaaatgga aaaagaatta ggaaaaacac taagaagatt acgtaaagga aaaaaagtta
     6421 gtatttcttc tttagcagat gagcacctat ccaagtcaca aatctcaagg tttgagagag
     6481 gagagtcaga aattacttgt agtcgtttgc ttaatatttt agataaactc aacatcacta
     6541 ttgatgaatt tgtgtcgatt cattctaagg cgcacactca tttttttatc cttttaaatc
     6601 gagtaagaaa atattgtgct gaaaaaaatg taactaaatt agtggctctc ttagaggatc
     6661 ataaccataa agactatgaa aagattatga ttaaggctct tattttttca attgatcaat
     6721 caatagagcc taatcaagaa gaattagcac gcttaacaga ctatcttttt acggtggagc
     6781 agtggggata ttatgaaatt attctcttag gaaattg
//
LOCUS       DATRNK010000078         6789 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.078, whole genome shotgun sequence.
ACCESSION   DATRNK010000078 DATRNK010000000
VERSION     DATRNK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.078"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            95..334
                     /locus_tag="VBC52_001802"
     CDS             95..334
                     /locus_tag="VBC52_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6883361.1"
                     /translation="MTLFLDSLLKELIIVNRLREYRIKASISQLALAKSVGVARQTIN
                     LIENNKYNPSLDLCIRLAECLQTDLNTLFWDSRHL"
     gene            434..967
                     /locus_tag="VBC52_001803"
     CDS             434..967
                     /locus_tag="VBC52_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEO6883362.1"
                     /translation="MKEKKNLGIKLIKSLYGINGPFDEYREQKINYLGNLLFATTSLY
                     HILSSIIAWFTVSKYPTETAYTLLALTFFLYFCIFLIVIPSYLNSHDLITPLEVSKND
                     YPVAIKKNKQKFIKIGIFYTVIQWLILGLTTNNNTPLIVSLFSLKVILASIMSGVAYN
                     GVMYLIFKNNVDIEKDE"
     gene            complement(1043..1537)
                     /gene="tpx"
                     /locus_tag="VBC52_001804"
     CDS             complement(1043..1537)
                     /gene="tpx"
                     /locus_tag="VBC52_001804"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEO6883363.1"
                     /translation="MTTFLGNPVTFTGKQLQVGDIAKDFLLIATDLSQKSLKDFEGKK
                     KVISVVPSIDTGICSKQTRTFNEELSELDNTVVITVSMDLPFAQKRWCSAEGLDNVIL
                     LSDFYDHSFGQEYALLMNEWHLLTRAVLILDEHNKVTYTEYVDNVNSDVDYEAAINAA
                     KILP"
     gene            complement(1610..2269)
                     /locus_tag="VBC52_001805"
     CDS             complement(1610..2269)
                     /locus_tag="VBC52_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEO6883364.1"
                     /translation="MDLFLSLERKFKAASDKEVSKQQEAYLRHHFKCYGIKSPERRML
                     YKELIKAAKRQAKIDWQLLDKCWQSDYREYHHFVLDYLLAMSQFLTYNDCSRLEFYAR
                     HQQWWDSIDVLTKIFGNLSLKDDKVMNLLSEWSLDQDFWMRRLAIEHQLGFKEKTNTD
                     ILSLFILRNTGSQEFFINKAIGWALRGYSKYNKVWVKDFISNYYDELSTLSIREGSKY
                     L"
     gene            complement(2269..3033)
                     /locus_tag="VBC52_001806"
     CDS             complement(2269..3033)
                     /locus_tag="VBC52_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO6883365.1"
                     /translation="MDFVFDIDGTLCFDGMSLSKEIQGILERAQIDYGHRVTFATARS
                     YRDTIGILGDKLSLSKIIGLNGATLHENGHLVDSYYLQSDFFSTIISYCHRHQIPYFV
                     DEVFNYATYQASKIPFIAYVDPQKRGELLEVSKIEKPIKMVLYFGDQLGRADQMLAEL
                     NRFGLSSHFFHEFEKCLYINPIAVDKGKATKKLFGNRFIAFGNDKNDISMFDAAHYSV
                     QVGDFDELTPYANLRVSRESVHEGITTLFEKYKGIT"
     gene            complement(3038..3679)
                     /locus_tag="VBC52_001807"
     CDS             complement(3038..3679)
                     /locus_tag="VBC52_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6883366.1"
                     /translation="MVSEQSEIVTSKYQKIAVAVAQRIANGDYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLQILTLKHGSGAIILSKEKAIEFLNQYETSHSVAILKGKIR
                     DNIKAQQQEMEELATLVDDFLLQTRAVSKQYPLAPYEIIVSEDSEHLGKSIGELNVWH
                     QTGATIVAIEHEGKFIVSPGPFSVIEQGDHIFFVGDEDVYARMKTYFNLRMGL"
     gene            complement(3843..4394)
                     /locus_tag="VBC52_001808"
     CDS             complement(3843..4394)
                     /locus_tag="VBC52_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6883367.1"
                     /translation="MENQKQDIKTSVTYEEKVIAKIVGHALESVDGLLAVSGGFFSNL
                     KNSVVNSDSVTDGVNVEVGTKEVAVDLDIVVEYGKDIPAIVESIKAIVSQNVEVMTHL
                     KVVELNANVVDIKTKAEHEADSVTVQDRVSDAAQATGNFASEQAGKAKAAISSGAEKT
                     KEAVSNGTEAAKEKISEARTSES"
     gene            complement(4416..4613)
                     /locus_tag="VBC52_001809"
     CDS             complement(4416..4613)
                     /locus_tag="VBC52_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6883368.1"
                     /translation="MSEEKFDAKVDKVSGSVKESVGKLTGDKEVESEGKVDKLKGHAK
                     EKLADIKDTIKGASESFKKKD"
     gene            complement(4660..5202)
                     /locus_tag="VBC52_001810"
     CDS             complement(4660..5202)
                     /locus_tag="VBC52_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6883369.1"
                     /translation="MTETYIKNTTNNSGTTAVRGELTFEDKVIEKIVGIAIEHVDGLL
                     AVNGGFFSNLKNSVVNSDSVTDGVNVEVGKKQVAVDLDIVAEYQKHVPTIFADIKKVV
                     EAEVKRMTDLEVVEVNVNVVDIKTRAQHEEDSVTLQDRVTNAAQATGEFASNQVSNVK
                     SAVGSGVDKVEDMKSEPRVQ"
     gene            complement(5258..5452)
                     /locus_tag="VBC52_001811"
     CDS             complement(5258..5452)
                     /locus_tag="VBC52_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEO6883370.1"
                     /translation="MSEFVRKYRYPLGGAVIGLVLAAMIVTIGFFKTILALVIIVLGA
                     YAGLYVQRTGMLDQFFNKRK"
     gene            complement(5462..6043)
                     /gene="amaP"
                     /locus_tag="VBC52_001812"
     CDS             complement(5462..6043)
                     /gene="amaP"
                     /locus_tag="VBC52_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEO6883371.1"
                     /translation="MSKGLKSLYTLLGLISLTLLGFVAVISKQHIYLPSFNWLDWDFN
                     LPSPIDVGMYHYFFWGALVLFVIVLLAILVVLFYPRRYTEYKLADKTGKLMLKKSAIE
                     GFVKTEVLKTGLMKSPSVTAHLYKKKVKVDVKGLLTSRTNVPEQLEHIQSDVELGLKE
                     FFGLEKKMNTRVFVKQVEEENVGNAKTNKSRVE"
     gene            complement(6058..6306)
                     /locus_tag="VBC52_001813"
     CDS             complement(6058..6306)
                     /locus_tag="VBC52_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO6883372.1"
                     /translation="MSLIWSLIVGAIIGAIAGAVTNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLAGMALIPSIVGAIIVVIVTSFVLGKMNN"
     gene            complement(6354..6599)
                     /locus_tag="VBC52_001814"
     CDS             complement(6354..6599)
                     /locus_tag="VBC52_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO6883373.1"
                     /translation="MGLIWSLIVGAIIGAIAGAITNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLADMALIPSIVGAIIVIIVTSFVLGKKS"
BASE COUNT         2228 a         1368 c          925 g         2268 t
ORIGIN      
        1 tccattttga ctggaaacaa gttttttata tttatttgtc acatataatt gacagtcttc
       61 gaaaaatgat atatactcaa aatgtcatct atatatgaca ctcttcttag attctttact
      121 aaaggagcta ataatcgtga atcgtcttag agaatacaga attaaagcta gcatatctca
      181 attagcttta gcaaaatcag ttggtgtcgc cagacaaaca ataaacctta ttgaaaataa
      241 taaatacaat ccttcgctag atctttgtat aagactagcg gaatgcctac aaacagacct
      301 caatactctt ttttgggata gtagacatct ataacagatt agactctcta aatctatgaa
      361 aactcgggag gttaggtcgt gttattattt caatctcaaa ctttattaaa tagttcaagg
      421 ataggagaat attatgaaag aaaagaaaaa tttggggata aaattaataa aatccctata
      481 tggtattaat ggcccattcg atgaatacag agaacaaaaa atcaattatc ttggaaatct
      541 attatttgca accactagcc tctatcacat tttatcctct atcatcgcct ggttcacggt
      601 aagtaaatat cccacagaga ctgcatatac tctcctagct ttaacattct ttctatattt
      661 ttgtattttt ctaattgtta ttccctctta tctcaacagt catgatttaa taacgccact
      721 agaagtctca aaaaatgact atccagtagc aataaagaaa aacaaacaaa aatttatcaa
      781 aatcgggata ttttatactg tcatccagtg gcttatcttg ggtttaacaa ctaacaataa
      841 cactccgctg attgtatcgc tattttcttt aaaagttatt ttggctagta ttatgtccgg
      901 tgtagcttac aatggagtta tgtatctaat atttaaaaac aatgttgata ttgaaaaaga
      961 tgaataatga caacatttca tctctaattg ttctcactgt ctatataaat aataaggctg
     1021 gaaaattttc cagccttatt atttatggta atatctttgc agcattaatc gccgcttcgt
     1081 agtcaacatc tgaattaaca ttgtcaacgt attcggtgta agtcacctta ttatgctcat
     1141 ccaaaataag aactgcacgt gttaacaaat gccactcatt catcaaaaga gcgtactctt
     1201 gaccaaacga atgatcataa aaatctgata ataaaattac gttatcaagg ccttcagcac
     1261 tgcaccaacg cttctgagca aatggaaggt ccatagaaac agtaataacc actgtgttat
     1321 ctagctctga taattcctca ttaaaagtac gtgtttgttt tgaacaaata cctgtatcaa
     1381 tagaaggaac aacgctaata actttctttt tcccttcaaa atcttttaaa gatttttgag
     1441 ataagtctgt agctattagt aaaaaatctt ttgcaatatc cccaacttgt agctgttttc
     1501 ctgtaaaagt aacaggattt ccaagaaatg ttgtcataag tttacctcct tatcaattac
     1561 ctttatttta ctatgaagct tcttcttttt cgaataattg aactgataat tagaggtact
     1621 tacttccttc gcgtatgctc aaagtcgaca actcatcata ataattactg ataaagtcct
     1681 ttacccatac tttattatat tttgaataac ccctaagcgc ccagccaatc gccttattga
     1741 taaaaaattc ttgacttcct gtatttctta aaataaaaag tgacaaaata tcagtatttg
     1801 ttttttcttt aaatcctagt tgatgctcaa tagctagtcg cctcatccaa aaatcttgat
     1861 caagtgacca ttctgatagt aaattcataa ccttgtcatc ttttaaggat aagttcccaa
     1921 aaatcttagt cagaacatca atactatccc accactgttg atgacgagcg taaaactcta
     1981 gtctagagca atcattatag gtgagaaatt gactcatagc taatagataa tcaagtacaa
     2041 aatgatgata ttcacgatag tcactttgcc aacatttatc aagcaattgc caatcaattt
     2101 ttgcttgtct tttagcggcc tttataagct ctttgtagag catacgtcgc tcaggtgatt
     2161 taataccata gcacttaaaa tgatggcgta aataagcctc ctgttgctta gaaacttctt
     2221 tgtcacttgc agctttaaat ttcctctcta aacttaggaa taaatccatt acgtaatccc
     2281 cttgtatttt tcaaaaagcg ttgtaattcc ttcatgtaca gattctcttg atacacgtaa
     2341 attagcgtaa ggagtcagtt catcaaaatc ccctacttga acactataat gcgcagcatc
     2401 aaacatagaa atatcatttt tatcattgcc aaaagctata aaacgattcc caaataattt
     2461 cttagtggct tttcccttgt caactgcaat gggatttatg tacaaacatt tttcaaattc
     2521 gtggaaaaag tgactactaa gtccaaaacg atttaattcc gctaacattt ggtctgccct
     2581 tcccagctga tcaccaaaat acaacaccat ttttatcggt ttctcaattt tagaaacttc
     2641 tagaagctct cccctttttt gaggatcaac ataagcaata aagggaattt tagaagcctg
     2701 ataagtagcg taattaaaga cctcatcaac aaaatatgga atctgatggc gatgacaata
     2761 tgaaataata gtactaaaaa agtcactttg taaatagtaa ctatccacta agtgaccatt
     2821 ttcatgtaaa gtcgcaccat ttaaaccaat aatttttgat aaagatagct tgtcgccaag
     2881 gatacctata gtgtctctat aagatcgcgc tgtagcaaaa gtgactctat gaccataatc
     2941 aatctgggct ctctctagta ttccttgtat ttctttagat agactcatac catcaaaaca
     3001 aagagttcca tctatatcaa agacaaaatc cattctctca aagtcccatc cttaagttaa
     3061 aataggtttt catacgtgca taaacatctt catcaccaac aaaaaaaata tgatctcctt
     3121 gctctataac tgagaaaggc cccggagaaa caataaattt cccttcgtgt tcaatagcca
     3181 caatagtcgc accagtttga tgccatacat ttaattctcc tattgactta ccaaggtgtt
     3241 cagaatcttc actgacaata atttcatatg gtgctagtgg gtattgttta ctaaccgcac
     3301 gtgtttggag caaaaaatca tcaactaaag tagctaactc ttccatttct tgttgctgag
     3361 ccttgatatt atcacggatt ttacctttca aaatagcaac tgaatgagag gtttcatatt
     3421 ggtttaagaa ttcaattgct ttttctttag agagaataat ggctccactg ccatgcttta
     3481 aagttaaaat ctgtaaatct gctaaaatat taagaccctt acgtgctgtt tctggtgata
     3541 cgttgaatgt tgaagcaata gttgttctag atttcaactt ctctccaact tcataatctc
     3601 catttgcgat tctttgtgca accgctactg ctattttttg atatttagat gttacaattt
     3661 cagattgttc agaaaccatc caaccctcct tatcctaata aaaacataca tattaaacca
     3721 caattagcaa aatattgcaa atatatcttt tttgttaata tctaatgtct ttagtaaata
     3781 tcatataaaa ggagctggtc tcccagctcc gagtgtctta tcataaaaag tagtttactg
     3841 aattaagatt cactagtacg agcttctgag attttttctt ttgcagcctc agtaccattt
     3901 gatactgctt cttttgtttt ctctgctcca gaggaaattg ctgctttagc tttgccagcc
     3961 tgttcagaag caaagtttcc tgttgcttgt gctgcatcag atacacgatc ttgtactgtc
     4021 actgagtcag cttcatgttc agctttagtc ttaatatcta cgacatttgc gttcaattca
     4081 acaactttaa ggtgggtcat cacttcaacg ttttgactga caatcgcttt aatgctttca
     4141 acgatagctg ggatgtcctt gccgtattca acaacgatgt ctaaatcaac tgcaacttct
     4201 ttagtaccaa cttcaacatt tacaccatca gttactgagt cagaatttac aacgctattt
     4261 tttagattag agaagaaacc tccactcaca gctaagagtc cgtctactga ttctaaagca
     4321 tgtccaacaa ttttagcaat taccttttct tcataagtta ctgaagtttt aatatcttgt
     4381 ttttgatttt ccataaataa atgacctcaa accttctagt cctttttctt aaagctctct
     4441 gaagcaccct taatggtatc tttaatgtca gcaagttttt cttttgcatg acctttcaat
     4501 ttatcaactt taccttcaga ttcaacttct ttatcgccag taagtttccc aacagattct
     4561 tttactgaac cagaaacctt atcaactttt gcatcaaatt tttcttcaga cataatagcc
     4621 tcctactaat aacaatactt taaactacac ttaaatcaat tattgtacac gtggttcaga
     4681 cttcatgtct tccactttat caacaccgct acctacggca cttttaacgt ttgaaacttg
     4741 attcgaagca aattcacctg ttgcttgtgc tgcatttgtt acacgatctt gaagagttac
     4801 tgaatcttct tcatgttgag cacgagtttt aatatcaaca acattaacat taacttcaac
     4861 cacttcaagg tctgtcatac gtttcacttc agcttcaaca acttttttga tatcggcaaa
     4921 gatagttgga acatgttttt ggtattcagc aacaatatcc aaatcaactg caacttgctt
     4981 tttacccact tcaacattta cgccatctgt aactgaatca gaatttacaa cgctattttt
     5041 taaatttgag aaaaaaccac catttacagc caaaagacca tctacatgct caatcgcaat
     5101 tccaacaatt ttttcaatca ctttatcttc aaaagttaat tctccacgaa cagctgtcgt
     5161 gccagaattg ttagttgtgt ttttgatgta agtttcagtc ataatagttc ctctttcttt
     5221 tatttaaata atgtagaata aatataaacg aaaaatttta ttttctctta ttaaagaatt
     5281 ggtcaagcat acctgttctt tgcacataaa gtccagcata agcacctagc acaatgataa
     5341 caagagcaag aattgtttta aagaatccaa tggttacaat catagcagct aaaacgagcc
     5401 caataactgc acctcctagt ggatatctgt atttcctcac gaattctgac atattagact
     5461 cctattctac acgactttta ttcgtcttag catttccaac attttcttct tcaacttgct
     5521 taacaaatac tctagtgttc attttctttt ccaacccaaa gaattctttt aagccaagtt
     5581 caacatcact ttgaatatgt tcaagttgtt ccggtacatt tgtacgagaa gttaaaagac
     5641 ctttaacatc aactttcact tttttcttat ataaatgagc tgtcacacta ggagatttca
     5701 tcaaaccagt ttttaatact tctgttttaa caaatccctc tattgcagat ttttttaaca
     5761 tcaatttacc agttttatca gctagtttat actctgtgta tcgtcttgga taaaacagaa
     5821 ctaccaatat agccagtaaa acgattacaa acagcactaa tgctccccaa aagaaataat
     5881 gatacatacc aacgtcaatc ggtgatggca aattaaaatc ccaatctaac caattaaaag
     5941 atggtagata aatgtgttgt ttactgataa cagctacaaa tcctagtagg gtaagggaga
     6001 ttagacctag taaagtatat aaacttttta gtccttttga cataggtaac tccttttcta
     6061 gttattcatt ttacctagta caaatgaagt aacaatgaca acaataatcg cacctacgat
     6121 tgaaggaatc aaagccatac ctgctaattt aggcccccat gttcctagta aactttgacc
     6181 aacaaatgaa ccaactaaac cagctaagat gttagctatc caacccattg atccaccttt
     6241 gttagtaaca gcccctgcaa tagcaccgat aatagcacct acgattaatg accaaattaa
     6301 actcatgata aataccttct ctttcttgac taatcattcc actagttatg tagttatgat
     6361 tttttgccta atacaaatga agtaacaatg ataacgataa tcgcacctac gattgaagga
     6421 atcaaagcca tatctgctaa cttaggcccc catgttccta gtaaactttg accaacaaat
     6481 gaaccaacta aaccagctaa gatgttagct atccaaccca ttgaaccacc cttgttagtg
     6541 attgctcctg caatagcacc aataatagca ccaacgatta atgaccaaat taaacccatg
     6601 ataaatacct ttcttcctaa gtacatctca gtgtacaaac ttattttgtt cttaactaaa
     6661 taacaagagt ttcttaagtg accactcggc aattaattaa gttgttaact atactaatat
     6721 tatacaactt tataaagtga ctgccaagta aaaagcttgg taaatgcttt tatttttaag
     6781 tgaccactt
//
LOCUS       DATRNK010000079         6756 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.079, whole genome shotgun sequence.
ACCESSION   DATRNK010000079 DATRNK010000000
VERSION     DATRNK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.079"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            32..661
                     /gene="udk"
                     /locus_tag="VBC52_001815"
     CDS             32..661
                     /gene="udk"
                     /locus_tag="VBC52_001815"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEO6883374.1"
                     /translation="MRKKPIIIGVTGGSGGGKTSVSRAILSNFPDQKITMIEHDSYYK
                     DQSHLTFEERVKTNYDHPLAFDTNLMIEQLNELIEGRPVDIPVYDYTKHTRSDRTIRQ
                     EPQDVIIVEGILVLEDQRLRDLMDIKLFVDTDDDIRIIRRIKRDMEERDRSLDSIIEQ
                     YTEVVKPMYHQFIEPTKRYADIVIPEGVSNIVAIDLINTKVASILNEAK"
     gene            748..1245
                     /locus_tag="VBC52_001816"
     CDS             748..1245
                     /locus_tag="VBC52_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEO6883375.1"
                     /translation="MNKSKKIENYQLLLLQAQALFSDETNALANLSNASAMLNAMLPN
                     SVFTGFYLFDGEELILGPFQGGVSCVHITLGKGVCGESAQTAKTLIVDDVTKHANYIS
                     CDSKAMSEIVVPMFKNGKLLGVLDLDSSLVADYDEIDQEYLEKFVGILVEHTIWNLDM
                     FGVEK"
     gene            1245..2909
                     /gene="dnaX"
                     /locus_tag="VBC52_001817"
     CDS             1245..2909
                     /gene="dnaX"
                     /locus_tag="VBC52_001817"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEO6883376.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVSSKKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQINGEPCNHCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKLLAIRDHLAQILDKEAISYDLDALTLVARRAEGGMRDALS
                     ILDQALSLAKDNHISLDVAEEITGSISLSAIDDYVSNILAHDTTEALAKLEVIFDSGK
                     SMSRFATDLLMYLRDLLVVQAGGEDSHSSDTFIANLNVKQDILFEMIDKVTSVLPEIK
                     NGSHPKVYAEMMTIQLSEMVEKNSSNIPADVTAELDSLRRELKSLKNEMSQLSRADQS
                     SSTQKVKVNNKTFTFKVDRTKILTIMEETVVDSQRSREYLEALKSAWNEILDNITAQD
                     RALLMGSEPVLANSENAILAFDAAFNAEQAMKRTDLNDIFGNIMSKAAGFSPNILAVP
                     RNDFNQIRSDFAKKMKAQKTETEPEVNHQIPEDFSYLAERIAIVED"
     gene            2998..3192
                     /locus_tag="VBC52_001818"
     CDS             2998..3192
                     /locus_tag="VBC52_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEO6883377.1"
                     /translation="MTKRQFIFMALLCSFETYFFNQSVMDGSWIFAIFWGVLLLRDLQ
                     KVYAISKFTKELIKSTKKKD"
     gene            complement(3173..4108)
                     /locus_tag="VBC52_001819"
     CDS             complement(3173..4108)
                     /locus_tag="VBC52_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEO6883378.1"
                     /translation="MKTYEKIYQILSKNNNFISGETMANQLNISRTAIWKGIKTLEEL
                     GLEIESVTNKGYRLVSGDILLPEQLEQEIGIKVSLNNNSASTQLDAKMGIESKLKTPH
                     LFLAPNQKKAKGRFDRPFFTSNQGGIYMSLLLQPNVPIEDIKPYTVMVASSAVKAISR
                     LTGITPEIKWVNDIYLDNKKIAGILTEAIASVESGLVTNVIIGLGINFYIKEFPRALT
                     KRAGSLFTEQPTITRNQLITEIWNLFFNIPLEDHLKVYREKSLVLDRTVSFMDGQTMY
                     SGKAIDITDKGYLVVELDDGQLKTLRSGEISLSSW"
     gene            4293..5489
                     /locus_tag="VBC52_001820"
     CDS             4293..5489
                     /locus_tag="VBC52_001820"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEO6883379.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILEQDPDAHVAAE
                     TAVYTGSVHVFGEISTTAYVDINRVVRNTIAEIGYDKAEYGFSAESVGVHPSLVEQSP
                     DIAQGVNEALEVRGSLEQDPLDLIGAGDQGLMFGFAVDETPELMPLPISLAHQLVKKL
                     TDLRKSGELTYLRPDAKSQVTVEYDENDQPIRVDAVVISTQHDPNVTNDQLHKDVIEK
                     VINEVIPSHYLDDQTKFFINPTGRFVIGGPQGDSGLTGRKIIVDSYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKVEVQLAYAIGVAQPVSVRVDTFGTGVIA
                     EADLEAAVRQIFDLRPAGIINMLDLKRPIYRQTAAYGHMGRTDIDLPWERVDKVQALK
                     DFIASK"
     gene            5476..5751
                     /locus_tag="VBC52_001821"
     CDS             5476..5751
                     /locus_tag="VBC52_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883380.1"
                     /translation="MLANNKKRKRKFTLFLLMIIYRTFIKSYNKLVKYRKYCIFLENM
                     VYIVNKMMAKLIEFSYRKIFQKNNFTLKSFDYQINIAFIKILYKYKM"
     gene            6009..>6756
                     /locus_tag="VBC52_001822"
     CDS             6009..>6756
                     /locus_tag="VBC52_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883381.1"
                     /translation="MKKQFLKSAAILSLAVTAVSTSQPVGAIVGKDETKLRQQLGYID
                     SKKSGKKIDERWGEKIYNYLSYELIEANEWINRSEFQEPEYRTILSEFKDKIDSIEYY
                     LINLSNIAKEDAHQRNILQSLDKYEKSGIYNLDQGVYNYIYQEISSAKHKFSDGVDKI
                     YRLDSTLFPFSVWYDKHLDNNDNYKDNKDFKEYIALLNEITRKARLGYQIVNNHKDGE
                     HKDEAEILDILIRDITFVSKDAPGYKYIPNK"
BASE COUNT         2303 a         1013 c         1262 g         2178 t
ORIGIN      
        1 gatagaggat aaatttaatt tggagtgaca aatgcgtaaa aaacctatta ttattggtgt
       61 tacaggtgga tctggtggtg gtaaaaccag tgtttctcgg gcgattttat caaatttccc
      121 agatcaaaaa attaccatga ttgaacatga ttcttactat aaagatcaaa gtcatttaac
      181 ttttgaagaa cgtgtaaaaa ccaattatga tcatcccctt gcttttgata ccaatttgat
      241 gattgagcaa ttgaacgaat tgattgaggg acgtccggtc gatattccgg tttatgatta
      301 cacaaaacat acccgtagtg atagaactat ccgccaagaa ccccaggatg tcattattgt
      361 tgagggaatt ttggtattag aagatcagcg tttgcgtgat ttaatggata tcaaattatt
      421 tgttgataca gatgatgata ttcgtattat tcgtcgtatt aagcgtgata tggaagagcg
      481 cgatagaagt ttagatagta ttattgaaca gtatacagaa gttgtaaaac ctatgtatca
      541 tcaatttatc gaaccaacaa agcgttatgc ggatattgta attccagaag gagtaagtaa
      601 tattgtcgct attgatttaa tcaataccaa agtggctagc atcttaaatg aagcaaaata
      661 aacacaatgg tttgtgttta tttttttgtt ttatatagga tggttattta gttttagtaa
      721 ctttcaaatt atgctataat aagtgctatg aacaagtcta agaaaatcga aaattatcaa
      781 ttattattac tacaagcgca agctctattc tcagatgaaa caaatgctct tgccaactta
      841 tcaaatgctt cagctatgct aaatgctatg cttccaaatt ctgtatttac aggcttttat
      901 ttatttgatg gagaagagtt aattcttggc cctttccagg gtggtgtatc atgtgtgcat
      961 attactttag gaaaaggtgt ttgtggtgaa tctgcacaaa ctgctaagac gctgatcgtt
     1021 gatgatgtta caaagcatgc taactatatc tcctgtgatt caaaagctat gagtgaaatc
     1081 gtagtaccta tgtttaaaaa tggcaaactt ctaggagttc tagatttaga ttcttcttta
     1141 gtagcagatt atgatgagat tgatcaagaa tacttagaaa aatttgtagg tattctagta
     1201 gaacatacga tttggaattt ggatatgttt ggagttgaaa agtaatgtat caggcccttt
     1261 atcggaaata tcgtagccaa acatttgatg aaatggttgg acaaagtgtt atttcaacta
     1321 ctttaaagca agctgtgtcc agcaaaaaaa ttagccatgc ttaccttttt tcaggaccta
     1381 ggggaactgg aaaaacaagt gctgctaaaa tatttgcaaa agcgatgaat tgcccaaatc
     1441 aaattaatgg cgaaccttgt aatcattgtg atatttgtcg agatattaca aatggtagtt
     1501 tagaggatgt cattgaaatt gatgctgcgt caaataatgg tgtcgatgaa attcgtgata
     1561 tcagggacaa atcaacttat gctccaagta gggcaaccta taaagtttat attattgatg
     1621 aggttcatat gttgtctaca ggtgccttca atgcactatt gaaaacactt gaagagccaa
     1681 cagaaaatgt tgtttttata ttagcaacaa cagaactcca taaaatccct gcaacaatct
     1741 tatctcgtgt ccagcgcttt gaatttaaag cgattaaatt attagctatt cgagatcatt
     1801 tagcacagat tttagataaa gaagctattt catatgattt ggatgctctg acactagtcg
     1861 ctagacgagc cgaaggtggt atgcgtgatg ctttatcaat tctcgatcaa gccttgagtt
     1921 tagcgaaaga taaccacatt tctttagatg tagcagaaga aattacaggg agcatttcct
     1981 tatctgctat agatgattat gtatcgaata tattggctca cgatacaact gaagcactcg
     2041 ctaaacttga agtaatcttt gatagtggca aaagtatgag tcgttttgca acagatttat
     2101 tgatgtactt aagagattta cttgttgttc aagcaggtgg tgaagatagt cattcaagtg
     2161 atacttttat agccaatctt aatgttaaac aagatatttt atttgaaatg attgataaag
     2221 tgacatctgt tcttcctgag ataaaaaatg gtagccatcc taaagtttat gcagagatga
     2281 tgaccatcca attgtctgaa atggtagaaa aaaattcgtc aaacataccc gcagatgtga
     2341 cagcagaatt agatagtctt cgtcgagagt taaaatcgtt aaaaaacgaa atgagccaac
     2401 tctctcgagc agatcaaagt tcatcaactc aaaaagtaaa ggttaataat aagacattca
     2461 catttaaagt tgaccgtaca aagattttga ccattatgga agaaacggtt gtagatagtc
     2521 aacgttcacg tgaatactta gaagctttaa agagcgcttg gaacgagata ttagataata
     2581 ttacggctca agatagagcc ttattaatgg gatctgaacc tgtcttagct aatagcgaga
     2641 atgctatttt ggcatttgat gcggccttta atgctgaaca agctatgaaa cgtaccgatc
     2701 tcaatgatat atttggtaat attatgagta aagcagcagg tttttcacct aatattttag
     2761 ctgtacctag aaatgatttt aatcaaattc ggagcgattt tgcaaagaaa atgaaagctc
     2821 aaaagacgga aacagaacct gaagttaatc atcaaattcc agaagacttt tcttatctag
     2881 cagaacgaat agcaatcgtt gaagattaaa acgtaactaa aaatagttgc ctgttacagg
     2941 caactatttt tttgataagt tttttaatta tttagtagat tctgttataa tggtgatatg
     3001 acaaaacgac aatttatttt tatggcgctt ttatgctctt ttgaaactta tttttttaat
     3061 caatccgtta tggatggctc ctggattttt gctatttttt ggggagtttt attacttcga
     3121 gatcttcaaa aggtgtatgc gatttcaaaa ttcacaaaag agcttatcaa atctaccaag
     3181 aagaaagact gatttcgcca ctgcgaagtg ttttcaactg accatcatct aactcaacca
     3241 ctaaatatcc tttatctgta atatcaatag ctttcccaga atacattgtt tgaccatcca
     3301 taaagctaac ggttctatct aagactaggg atttttctct atataccttt aagtgatcct
     3361 ctagtggaat attgaaaaag agattccaga tttctgtaat caattgattt ctagttatgg
     3421 tcggttgctc tgtaaagaga ctacctgcgc gcttagttaa cgccctaggg aactctttga
     3481 tatagaaatt gatacctaat cctataataa cattcgttac caacccactc tcaacagaag
     3541 cgatagcctc ggttaaaata ccagctattt ttttattatc taaataaata tcattgaccc
     3601 acttaatttc aggagtaatt cctgttaacc gtgagatagc ttttacagca ctagaggcta
     3661 ccatgacagt ataaggtttt atatcttcaa tcggtacatt aggttgtaat aaaagagaca
     3721 tatagatgcc gccttgattt gatgtaaaga aaggtctatc aaaacgtcct ttagcttttt
     3781 tttgattagg agctaaaaaa aggtgtggag tctttaattt actttctatc cccattttag
     3841 catctagttg ggttgaagca ctattattat taagactaac cttaatacca atttcttgtt
     3901 cgagttgttc tggtaataat atgtcacctg ataccaatcg gtagcccttg ttagtgacag
     3961 actcaatctc taggcctaat tcttctaacg tttttatacc tttccaaata gctgtgcggg
     4021 aaatattaag ttgatttgcc atagtttctc cgctaataaa gttgttattt tttgatagta
     4081 tttgataaat tttttcgtag gttttcataa atctagtata gtataaatcg agaaaaaaga
     4141 ctaaagaaaa tagagatatt tttttattta ttggtaactt atttcacaaa agatgaaata
     4201 gtggtacaat agctatatga gtcccgaaaa ggtagcctag aggctagttc tttgtaactc
     4261 gacacatttt acattttagg ggggacattt ttatgtcaga acgtaaactt ttcacgtctg
     4321 agtcagtatc tgagggacat cctgataaga ttgctgacca aatttccgat gcgattttag
     4381 atgcaatttt agagcaagat ccagatgccc acgtagcagc cgaaacagct gtttatacag
     4441 ggtcggttca tgtatttggc gaaatatcaa cgacagccta tgttgatatt aaccgagttg
     4501 tccgtaacac aatcgctgaa attggctatg ataaagctga atatggcttc tcagctgaat
     4561 ctgttggagt gcatccgtca ctcgttgaac agtcgccaga cattgcacag ggagtcaatg
     4621 aagctcttga ggtacgtggt tctcttgaac aagatccttt agatttaatt ggtgcaggtg
     4681 accaaggttt gatgtttggg tttgctgttg atgaaacacc tgagttaatg ccattgccaa
     4741 tttctttagc tcatcagtta gttaagaaat tgacagatct acgtaagtct ggggaactca
     4801 cttatcttcg accagatgca aaatctcaag taacggttga atatgatgaa aatgatcaac
     4861 caattcgtgt ggatgcagta gttatttcga cacagcatga tcctaatgta actaatgacc
     4921 aactccataa ggacgtgatt gagaaagtta tcaatgaagt tattccaagc cattatttag
     4981 atgaccaaac aaaattcttt atcaatccta cagggcgctt tgttattgga ggccctcaag
     5041 gagattcagg actaacaggt cgtaagatta ttgtagatag ttatggtggt tattctcgtc
     5101 atggtggtgg tgcattttca ggtaaagatg ctactaaagt agatcgttca gcatcttatg
     5161 cagctcgtta tattgcaaaa aatattgttg cagctgattt agctaaaaaa gtagaagtac
     5221 aacttgctta tgcaattggt gttgcccagc ctgtctctgt gcgtgtagat acatttggaa
     5281 caggtgttat agcagaagca gacctagagg cagctgtacg tcaaatattt gatttgcgac
     5341 cagcaggcat tattaatatg ttggacctca aacgacctat ttatcgacaa acagcagcct
     5401 atgggcatat gggacgtact gatatcgatt tgccatggga gcgagttgat aaagtccagg
     5461 ccttaaagga ttttattgct agcaaataat aaaaagcgta aacgaaagtt tacgcttttt
     5521 ttgctcatga taatttatag aacatttata aaatcttata ataaactggt taagtatagg
     5581 aaatactgca tatttcttga aaatatggtg tatattgtga ataaaatgat ggccaagtta
     5641 attgaatttt cctatcgaaa aatttttcaa aaaaataatt tcacactcaa atcatttgat
     5701 tatcaaataa atatagcctt tataaaaata ttatataagt ataaaatgta aaaaaaataa
     5761 aaaatgatat ttttatttga tttaaatgta tttaataaaa atacaaagtt tctaaaaaaa
     5821 gtaaaaattc catctcgaca aacagcgtta gttattataa ccgaacatta ttgtccttaa
     5881 aacatgaaaa caaaaacaaa agttcgtaat ttaattgatt tgccatgtta ctaatcttat
     5941 gctaatatat tatctcgtga taagtttttg atgtaaaaat tatcatgaaa aagaaaagag
     6001 agatggaaat gaaaaaacaa tttttaaaat cagcagcgat tctatcgcta gcagtaacag
     6061 cagtatctac aagtcagccg gtaggggcga tagtgggaaa agatgaaacg aaattaagac
     6121 agcaacttgg gtatattgat tcaaaaaaat ctggaaagaa aattgatgag agatggggtg
     6181 aaaaaattta taactatcta agttatgagc tcattgaagc aaacgaatgg attaaccgaa
     6241 gtgagtttca ggaacctgag tatagaacta ttttatcaga atttaaagat aaaatagata
     6301 gtattgaata ctacttaatt aacttaagta atattgctaa agaagatgcg catcaaagaa
     6361 atattcttca atcattagat aaatatgaaa aaagtggaat atataattta gaccaaggtg
     6421 tttataatta tatatatcaa gaaattagtt cagcgaagca caagttttcg gatggggtag
     6481 ataaaattta tcgtttagat agtactttat ttcctttctc tgtatggtat gataaacatt
     6541 tagataataa tgataactac aaggataata aagattttaa agagtacatt gctttattaa
     6601 atgaaattac acgaaaagca aggttaggat atcaaattgt aaataaccat aaagatgggg
     6661 agcataaaga tgaagcagaa atactggata ttttaatcag agatataact tttgtttcaa
     6721 aagatgctcc tggatataaa tatattccaa ataaaa
//
LOCUS       DATRNK010000080         6450 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.080, whole genome shotgun sequence.
ACCESSION   DATRNK010000080 DATRNK010000000
VERSION     DATRNK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.080"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..904
                     /locus_tag="VBC52_001823"
     CDS             <1..904
                     /locus_tag="VBC52_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 52 protein"
                     /protein_id="HEO6883382.1"
                     /translation="LKDKENTRIFICDTITNYETWVKTLNEQGIRTEAVKEFAYREQL
                     QSKNIEEVMELVDSDLNHYFERVDTQVYLFNDDTLIGRYMVYLGKNYHLIEDGYNCFQ
                     AKLFLGGSVVKRVIKTYLFKKYVPYGFSKYCLSIEVNSLVGLPHDIRSKKYKELPRKK
                     LFDSLNKEQKSLIFKIFKTKPLTITPKSVLLLTQPLAQDKCYKTPTERFQSIQEQYDY
                     FDDIVQEYRTLGYNVYLKVHPRDVVDYSKLPVELLPSNIPMEIIELMLTGRFEFGITH
                     SSTALDFLTCVDKKITLVDLKDIK"
     gene            901..2301
                     /locus_tag="VBC52_001824"
     CDS             901..2301
                     /locus_tag="VBC52_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEO6883383.1"
                     /translation="MNTKKLLQSGFIYTLGNLLVQGLAFITLPIYTRVISTEVYGQYS
                     LYVAWMGIITLFIGLQTSGSLSSARVKYGEEFKSYSGSAFSVGNIWFLIVLLIAFLFR
                     SFLAPLVGFSESIFLLMVCQSYANYVVTFFGQYFIQQQRSLANLILSLANAVSSVALS
                     LFLIFHWSDDFLSRVFGAFVPTIITGIVAFAYIYYHSKSFYNPKYFRFIVTVSIPLIF
                     HLLGHQLLNQLDRIMLARLYSTKEVAMYSFGYSLGMIIQIVLNSINMAWIPWFFDARK
                     EKLLQLSTYISRYLYLGVFLTLGYLTVFPELAQIMGGDKYSSSVQFISLIIVSYFLVF
                     LYTFPVNIQFFYANTTWIPIGTLLAAGVNWLLNLVLIPHYAAYGAAMATIISYLALLI
                     FHHIVSKVKYHYSDVSVRQYIILSGIVFSYAMLMNMFLGNIVIRWSLGIIILIVYSVV
                     FQKVILDLLGKKRRRR"
     gene            2301..3326
                     /gene="neuB"
                     /locus_tag="VBC52_001825"
     CDS             2301..3326
                     /gene="neuB"
                     /locus_tag="VBC52_001825"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEO6883384.1"
                     /translation="MVYIIAEIGCNHNGDINLAKKMVDVAVSCGVDAVKFQTFKAEKL
                     ISKFAPKAEYQKATTGTADSQLEMTKRLELSFEEYLEMRDYAISKGVETFSTPFDEES
                     LEFLISTDMPIYKIPSGEITNLPYLEKIGKQQKKVILSTGMAVMEEIHQAVNILRQNG
                     TTDISILHCTTEYPTPYPSLNLNVIHTLKDEFKDLTIGYSDHSIGSEVPIAAAAMGAE
                     VIEKHFTLDTNMEGPDHKASATPDILAALVKGVRIVEQALGRFEKIPDPVEEKNKIVA
                     RKSVVALKPIKKGDIYSIENITVKRPGNGISPMNWYDILGQEAQDDFEEDEVIRDSRF
                     ENQLPEL"
     gene            3403..4557
                     /gene="neuC"
                     /locus_tag="VBC52_001826"
     CDS             3403..4557
                     /gene="neuC"
                     /locus_tag="VBC52_001826"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEO6883385.1"
                     /translation="MKKICLVTGSRAEYGIMKPLIQRLSKDKEVNLQIIATAMHLEEK
                     YGYTYRQIEEDGFDIAYKVPLHLYDTDRRTVSTAMAHLQLGLTKIFDKEDYDLVIILG
                     DRYEMLPVVNVALIYNVPVCHLHGGETSLGNFDEYIRHAITKMSHLHLVSTEDFRQRV
                     IQMGEQPQFVINTGALGVENALSIPSLTKEAIEKQLGIALEESYFVVLYHPVTFEQGK
                     SAGEQMKAVLSALSKFGVQCLFIGSNSDTGSDDIAKAINTYLTNHENSHCFASLSTQL
                     YHSLIRHSLGLIGNSSSGLIEVPSLMKPTLNIGDRQKGRLHGESVVSVPVETSSVLEG
                     LSKLNEVTNFDNPYYKENASSIAYEAIKLYLKDEPSISQPFYDLKENNLK"
     gene            4554..5183
                     /locus_tag="VBC52_001827"
     CDS             4554..5183
                     /locus_tag="VBC52_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO6883386.1"
                     /translation="MKKKLAFLGAGSHADAIAPVIDPLVYDFVGFFDDKDITEHDGYP
                     VLGKLYDVLPYLEDGSIDAVFITIGDNAKRKELFEYVAKDYYDFIINIISPNALVLTP
                     DSICGRGIFIGFGAFIGSKVKLFDNNVVNTGALIEHHTVVESHCNIAPNATINGLCYI
                     REEVYVGSASVIIQTLDISSCTTIGAGAVVVKDIIEPGTYVGVPAKKIK"
     gene            5194..6435
                     /gene="neuA"
                     /locus_tag="VBC52_001828"
     CDS             5194..6435
                     /gene="neuA"
                     /locus_tag="VBC52_001828"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEO6883387.1"
                     /translation="MKPICIIPARSGSKGLPDKNMLFLAGKPMIFHTIDAAIESGMFD
                     KKDIFVSTDSELYREICLERGISVVMRKPELSTDQATSYDMLKDFLSDYEDNQEFVLL
                     QVTSPLRKSWHIKEAMEYYSSHDVDNVVSFSEVEKHPSLFTTLSDEGYAIDMVGADKG
                     YRRQDLQPLYYPNGAIFISNKETYLREKSFFTSKTYAYQMAKEFSLDVDTRDDFIHVI
                     GHLFFDYAIREKENKVFYKEGYSRLFNREASKIILGDSKTISISLESYHNYSQGGVTL
                     ATMLENLPNFLTANVTEAFVSIGVNDLITGYSVEEIFSNFQKLYSLLAENKIKMRLTT
                     IAYTLFRETVNNADIEKINQWLTEFCYQNQIPLLDINRFLSKDGNLNYHLTSDGLHFT
                     QEANDLLQSQYQLFVDEVKTL"
BASE COUNT         2111 a          949 c         1207 g         2183 t
ORIGIN      
        1 gttaaaagat aaagaaaata ctagaatatt tatttgtgac actatcacga attatgaaac
       61 ttgggtaaaa acattaaatg aacagggaat aaggacggaa gcagttaaag agtttgctta
      121 tcgggagcag ttacaaagta aaaatattga agaagtgatg gagcttgtag attcagactt
      181 aaatcactac tttgagagag tagatacaca agtctatcta tttaatgatg atactttaat
      241 aggtcgctat atggtgtatt tgggtaaaaa ttatcatcta attgaagatg gttataactg
      301 ttttcaagcc aagttattct tgggtggttc tgtcgtaaaa cgagtaatta aaacttactt
      361 atttaaaaag tatgttccgt atggtttttc aaaatattgt ctctcaattg aggttaattc
      421 tttagtgggg ctacctcatg acataaggag taagaaatat aaagaactac cgagaaaaaa
      481 attatttgat agtcttaaca aagagcaaaa atcactgatt ttcaaaatat ttaaaacaaa
      541 accattaact ataactccaa agtcagtatt attgttgaca cagccacttg cacaagataa
      601 atgttataaa acacctacag agaggtttca aagtattcaa gagcaatacg attattttga
      661 cgatattgtc caggaatata gaacgttagg gtacaatgtt tatttaaaag ttcatcctag
      721 agatgtagta gattattcca aattgccggt agagctatta ccatcaaata tccctatgga
      781 aattatagag ttgatgttaa caggacggtt cgaatttggg ataacacatt cgtccaccgc
      841 gctggatttt ttaacttgtg ttgataaaaa aataacttta gtagatctga aggatattaa
      901 atgaatacta agaaattact tcaatctggt tttatttata cactcgggaa tttattggtg
      961 caaggattag cctttatcac acttcccatc tacactcgag tcatctcaac agaagtttat
     1021 ggccaatata gtttatatgt tgcttggatg ggaattatta ctctgtttat tggtcttcag
     1081 acgagcggtt ctttaagttc ggctcgagtc aaatatggag aagagtttaa aagctactct
     1141 gggagtgcct tttctgtagg taatatatgg tttcttattg tacttttgat agcttttcta
     1201 tttagaagtt ttcttgcacc attagttggt ttttctgaat ctattttttt attaatggtg
     1261 tgtcaaagtt acgctaacta tgtggtgact ttctttggtc agtattttat acaacaacag
     1321 aggagcttgg ctaatttaat actatcctta gccaatgcag tttcatctgt tgcactatct
     1381 ctatttttaa tttttcattg gtccgatgac tttttatcta gggtttttgg agcttttgtt
     1441 cctactataa taactggaat agttgccttt gcttatattt attatcatag caaatctttt
     1501 tacaatccta agtattttcg gttcattgtc actgtgtcta ttcctttgat ttttcatttg
     1561 ttaggacatc agttattaaa tcaattagat aggattatgc tagctcgctt atatagtact
     1621 aaagaagttg caatgtatag tttcggttat tcgcttggaa tgattattca gattgttttg
     1681 aatagtatta atatggcttg gattccatgg ttttttgatg ctagaaaaga aaaattactg
     1741 caactttcaa cttatattag ccgttatctc tatttaggag ttttcttaac attaggttat
     1801 ttgactgttt ttcctgagtt agctcaaata atgggaggag acaagtatag tagtagtgtt
     1861 caatttattt ctttaattat tgtcagctat tttttagtct ttctctatac atttcctgta
     1921 aatatccaat tcttctatgc taatacgaca tggattccta ttgggacatt attagctgct
     1981 ggtgtaaatt ggttactaaa ccttgtatta attcctcatt atgcagccta tggcgctgct
     2041 atggctacaa taatatctta tttagccttg ttgatttttc atcatattgt ttcgaaagtt
     2101 aaataccatt attcagatgt ctcagttagg cagtacatta ttttaagtgg tatagtattt
     2161 agttatgcta tgttaatgaa catgttccta ggtaatatcg ttattagatg gagtttagga
     2221 ataattattc ttatagtata tagcgttgtt tttcaaaaag tcattttaga cttattgggt
     2281 aaaaaaagaa ggagaagata atggtttata ttattgcaga gattggttgc aatcataatg
     2341 gagatattaa tcttgcgaaa aaaatggtag atgttgccgt gtcttgtggt gttgatgctg
     2401 ttaaatttca gacttttaaa gctgagaaac ttatttctaa atttgctccc aaagctgaat
     2461 atcaaaaagc aactacagga acagcagaca gtcaacttga gatgacgaaa cgtttagagt
     2521 taagctttga agaatactta gaaatgcgtg attatgcaat ttcaaaaggt gtggagacct
     2581 tttcaacacc ttttgatgaa gagtcattag agttcttaat ttctacagat atgccaattt
     2641 acaaaattcc atcaggagaa atcactaatt taccttactt agaaaagatt ggcaagcaac
     2701 aaaagaaagt tattctttcg acgggtatgg cggtaatgga agagatccat caagcggtga
     2761 atattttacg tcagaatggt acaaccgaca tttctatttt acattgtaca acagagtacc
     2821 caacacctta cccctctcta aatttaaacg ttattcatac tttgaaagat gaatttaaag
     2881 atttaacgat aggttattcg gatcattcaa ttggatcaga agtacctatc gcagcagcag
     2941 caatgggtgc agaagttatt gaaaaacact ttactttaga tactaatatg gaaggtccgg
     3001 atcataaagc cagtgcaaca cctgatattt tagctgcttt agttaaaggg gttcgcattg
     3061 ttgaacaagc cttaggtaga tttgaaaaaa tcccagatcc agtagaagaa aaaaataaga
     3121 ttgttgctcg taaatcagtc gttgctttaa aaccaattaa aaaaggcgat atttattcaa
     3181 tagaaaatat tacggtgaag cgcccaggta atggtatttc tcctatgaac tggtatgaca
     3241 tcttgggaca agaagcgcaa gatgatttcg aagaggatga agttattcgt gattcacgct
     3301 ttgaaaatca attgcccgag ttataataac acctaattat aatcggcaac agatgtatta
     3361 cacggctaag attatgtaat tactatatat agaagggata cgatgaaaaa aatttgttta
     3421 gtaaccggat cgcgtgctga atacggaata atgaagccat tgattcaaag attgtcaaaa
     3481 gataaagaag tcaacttaca aattattgca acagcaatgc atctggaaga aaagtacggc
     3541 tatacttatc gtcaaattga agaagacggt tttgatattg cttataaagt tcccttacat
     3601 ctttatgata ctgacagaag aactgtatct actgcaatgg cgcatttaca actaggattg
     3661 accaaaattt ttgacaagga agactatgat ctagtcatca ttttagggga tcgttatgaa
     3721 atgttaccag ttgtgaatgt agcgttgatt tataatgtcc cagtatgcca ccttcatgga
     3781 ggggagacct cattaggcaa ttttgatgag tatattcgcc atgcaattac taagatgagt
     3841 cacctacact tagtctctac agaggacttt cgtcaacgtg tgattcagat gggagaacaa
     3901 cctcaatttg taattaacac aggagctctc ggagtggaaa atgctctatc aatcccttct
     3961 ctaaccaaag aagcaataga aaagcaatta ggtattgcac tagaggaatc ctattttgtt
     4021 gttctctatc atcctgttac ttttgaacaa ggaaaaagtg ctggagagca gatgaaagcg
     4081 gtactatcag ccctttctaa gtttggtgtc cagtgtctat ttataggatc caattcagat
     4141 actggttctg atgatattgc aaaggctatc aatacttatt tgacaaatca tgaaaatagt
     4201 cattgctttg cttctttatc aacccaactt tatcattcct taattcgtca ttcactagga
     4261 ctgattggta attcgtcttc tggtttaata gaagttcctt ctttgatgaa acctactctt
     4321 aatattggtg atcgccaaaa aggtaggcta catggagaaa gtgtagtaag tgtaccagtc
     4381 gaaacctcct ctgtattgga gggattatca aaattgaatg aagtgaccaa ctttgacaat
     4441 ccctactaca aagagaatgc ttcatctatt gcatatgaag caataaagct gtatttgaaa
     4501 gatgaacctt caatttccca accattttat gatttaaagg agaataatct aaaatgaaaa
     4561 aaaagttggc atttttaggc gcaggaagtc atgctgatgc gattgctccg gttattgatc
     4621 ctcttgtgta tgatttcgta ggtttttttg atgataaaga tattacggag catgatggtt
     4681 atcctgttct tggaaaactt tatgatgtgc taccttacct tgaagatggc tcaatagatg
     4741 cagtatttat tacaataggt gataatgcta aaaggaaaga actatttgaa tatgtagcaa
     4801 aggattatta tgactttatt attaacatca ttagtcccaa tgctttagta ttgacaccag
     4861 atagtatttg tggacgtggt atctttattg gttttggggc ttttataggt tctaaagtga
     4921 agctgtttga taacaacgtt gttaatacag gagcgctcat tgaacatcat actgttgtag
     4981 aatcacactg taatatagca cctaatgcta ccataaatgg tctttgttat attagagaag
     5041 aagtttatgt aggtagtgcc agtgttatta ttcaaacctt ggatatttca tcgtgtacga
     5101 caattggagc aggagctgtt gttgtaaaag atattataga acctggaacc tatgtagggg
     5161 ttccagccaa aaaaataaaa taaaggatat gttatgaagc caatttgtat tattcctgcg
     5221 cgatcagggt caaaaggatt accagataag aatatgttat tcctagcagg taaacccatg
     5281 attttccata ctatagatgc tgcaattgaa tctggaatgt ttgataagaa agatattttt
     5341 gttagtacag attcagaatt gtacagagag atttgtttag aacgcggtat ttcagtggtg
     5401 atgagaaaac cggaactttc aactgatcag gcaacttcgt atgatatgtt aaaagatttt
     5461 ttatctgact atgaagataa tcaggagttt gtgttacttc aagtaacttc tccactaaga
     5521 aaatcatggc atataaagga agcaatggag tattattctt cacatgatgt tgacaatgtt
     5581 gtaagttttt ctgaagttga gaaacaccct agtctgttta caacattatc tgatgaaggc
     5641 tatgctatag atatggtggg agcagataaa ggttatcgtc gccaagattt acaaccttta
     5701 tactatccga acggcgctat ttttatttct aataaagaaa cttacttaag ggaaaaaagt
     5761 tttttcacct ctaagacata tgcttatcaa atggcaaagg aattttcatt agatgttgat
     5821 acgagagatg attttatcca cgtcatcggt cacctctttt tcgattatgc cattagggaa
     5881 aaagagaata aagtttttta taaagaaggc tatagtcgtt tgttcaatag agaagcttca
     5941 aagataattt taggtgattc aaaaacgata tctatctcac tagaaagtta ccataattat
     6001 tcccaaggtg gtgtaacatt agcaacgatg ttggaaaact tacctaactt tttgacagct
     6061 aatgtaacag aggcttttgt ttctataggg gtaaatgatc tcattacagg ttatagtgtg
     6121 gaagaaatat tcagcaattt ccaaaaactc tactcattat tagctgagaa taaaataaag
     6181 atgagattga caactattgc ttacaccctt tttagagaaa ctgtcaataa tgcagatatt
     6241 gagaaaatca atcaatggct aacagaattt tgttatcaaa atcagattcc attgttagat
     6301 attaatagat ttttatcaaa ggatggtaat ctaaattatc atttaactag tgatggattg
     6361 cattttactc aagaggctaa tgatttgtta caaagtcaat atcaattatt tgtagacgaa
     6421 gttaaaacct tataatattt attttataaa
//
LOCUS       DATRNK010000081         6431 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.081, whole genome shotgun sequence.
ACCESSION   DATRNK010000081 DATRNK010000000
VERSION     DATRNK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.081"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..758)
                     /locus_tag="VBC52_001829"
     CDS             complement(<1..758)
                     /locus_tag="VBC52_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883388.1"
                     /translation="MLDLEKILRGMISAKFKDLRGSIPPDIISNGQRTAIYNIEKGKN
                     PKSGNFVTDTLLEDYSSYFGMDKSDLIFGDALLLELTLYHVFSQIFYQIVPDDNRIGL
                     KIDRNKIQNNIDSSIIEPFLELFYIFGDFGRWYNIRRKDDDESDKDIDYTTMYEIVWR
                     LIKSRVVSSFNQHVVGEMFEKDKVFNFNQINNVFNNWYHKHFVKIIIPEALKKLESDS
                     IFKMGFMVRNLIENFLVLDLPLSYLEDVPLEEYY"
     gene            complement(810..1301)
                     /locus_tag="VBC52_001830"
     CDS             complement(810..1301)
                     /locus_tag="VBC52_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883389.1"
                     /translation="MAKSLQSQFRDIERNLVRETNKMVKRANKKIKPVILPTQLDSSK
                     TALNFNEYHQDNSTHVSVNGDLINSQIGGQNNALNITYTEIEIERVFENIREYSRTLD
                     EEEQTELVTILNEVQEVGKVDNHQTFFEKHPILAMALSASVSWGVENGIDRLVSVISN
                     TFK"
     gene            complement(1315..4926)
                     /locus_tag="VBC52_001831"
     CDS             complement(1315..4926)
                     /locus_tag="VBC52_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HEO6883390.1"
                     /translation="MAIKGITLKESMNRSVQAIMPLDEEKEVFNQKLVSYLTHLKDKE
                     DESEEYQKNLLKVFLESVLPYNFINTSSRIDLAIYNGKDANSSLGILFECKSLFNKSE
                     MMSTEKINSKAFQEIVYYYLQERLFNKNLEIKKCIITNGLSWFVIEAKEFEKHFFKNK
                     KLVDLVTKFRNNQLSSNKTDFLYSEVIAPEIDKAFEKGIVIAHFDLSQALVKTSKSIE
                     IKKNNLTQLYRFFTAENLLNKEIFTDSNKLNKNFYDELLYLMGLEETKSGTSKIISRL
                     KPIKRQRYSFVENIINKLEMKDVSKEKQEDIAIQLTVVWTNRILFLKLLESQLVLFNK
                     DESYRFLTYEKLPNFEEIYGLFFAVLAKKVSERNDRVQEKFGYVPYLNSSLFEETEIE
                     ISRDGIGIDRLPEGDIEIFSKTALKGVDKKRKKGNINFIEYLFEFLDSYDFSTSISHH
                     EKSKNDLINASVLGLIFEKINGYRDGSFYTPGNITMYMSRKAIRTAAVDKVNELLGWN
                     CETVEEIKFAIGHSVENARKVSQAIDDLKVCDPAVGSGHFLVSILNEIIALKSDLNIL
                     FDADGNYIGNLIQCYVINDELVIQDMSGNNFLYQTGNQQSEQIQKAIFNQKRHILENS
                     LFAVDINPSSVNICRLRLWIELLKSSYYYQDTDTNQPVLTTLPNIDISIKVGDSLLHK
                     FEFDYEFDMRKTDFKDYLSLVKDYKETNNKRVKAEIWEKIERLKHSFDDTASSPELKK
                     LSRLISERNKAGAISLFEDENKNSKEVAELNKQLAKAEKELEYRLKNPLFAKGMEWRM
                     EFPEILGEAGEFIGFDLVIGNPPYIFARNQSFTDEMKSYYSRTYQVSEYQANTYTIFM
                     ELAYQLLRKGGTFSYIIPNNFLTIQTNSRVRQFITEQTSDVVLINSLDKIFADASVDN
                     CIIFFKKNAPNWIEVAELYHGEFNTVGRVAPDFFGEIPTFSISMVKYRQAIDIFWKVN
                     HFPILGRPEISTVKSGIKAYETGKGNPKMTAQDKNNRIYHARERSDDSYRPYLDGENV
                     SRYKLTWNGEYVKYGENLAAPRDKEIFEKPRILVRQIPSKSTYAVEAVYTDSDVINDL
                     NSMVITDIQVNPFYLLGILNSRLISLWFFMKFDKFQRRLFPQFKVNELGDFPIPDATD
                     SQQEDIAQVVEQLMEEMKKDSPDTEKVNQLNLEIDELVMDLFDLAEEEKQTVRDFEV"
     gene            complement(5167..5532)
                     /gene="rplL"
                     /locus_tag="VBC52_001832"
     CDS             complement(5167..5532)
                     /gene="rplL"
                     /locus_tag="VBC52_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEO6883391.1"
                     /translation="MALNIENIIAEIKEATILELNDLVKAIEEEFGVTAAAPVAAAAA
                     SGEAAAAKDSFDVELTAAGDKKVGVIKVVREITGEGLKEAKAIVDNAPSVIKEGASEA
                     EANEIKEKLEAAGASVTLK"
     gene            complement(5596..6096)
                     /locus_tag="VBC52_001833"
     CDS             complement(5596..6096)
                     /locus_tag="VBC52_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEO6883392.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVEQDTNLRR
                     SLRESDVEFKVIKNSILIRAAEKAGLEDLKELFVGPSAVAFSNEDVIAPAKVISDFAK
                     DAEALEIKGGSVDGKFTSVEEINALAKLPNKEGMLSMLLSVLQAPVRNVAYAVKAVAE
                     KDEEVA"
     misc_feature    complement(6126..6259)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
BASE COUNT         1986 a         1228 c          964 g         2253 t
ORIGIN      
        1 aagtagtatt cctctaatgg aacatcttct aaatatgata gtggtaaatc taaaaccaaa
       61 aaattttcaa tcaaattcct aaccataaat cccatcttaa aaatactgtc agactccaat
      121 ttttttaacg cctcagggat tatgattttt acaaaatgct tatgatacca attattaaag
      181 acattgttga tttgattaaa attaaatacc ttgtcttttt caaacatttc tccaactaca
      241 tgttgattaa atgaactaac cactctactt tttatcaaac gccaaacgat ttcatacatt
      301 gtcgtataat ctatatcttt atctgattca tcatcatcct tccttctaat attataccat
      361 cgcccaaaat ccccaaaaat atagaatagt tctaaaaatg gctcaataat gcttgaatca
      421 atattgtttt gaattttgtt cctatcaatc ttcaagccaa tacgattatc atctggtact
      481 atttgataaa aaatttgaga aaatacatgg taaagagtca gttctaataa tagagcatct
      541 ccaaagataa gatctgattt atccattcca aaatagctag agtaatcctc cagtaaagta
      601 tcggttacaa aatttccgct tttaggattt ttaccttttt caatgttgta gattgcagtt
      661 ctttggccat ttgaaatgat atctggtgga atgcttcccc tcaagtcctt gaattttgca
      721 gaaatcattc ccctcaaaat tttctctaag tccaacatca tctacacctc aaactctcca
      781 ttttctattt ccatcaaata aaaaactttt cacttaaatg tatttgaaat gacactaact
      841 aatctatcaa ttccattctc cactccccag cttacactag cactaagagc cattgcaagt
      901 atagggtgtt tttcaaaaaa agtttgatgg ttatcaactt ttccgacttc ttgtacctca
      961 tttaaaattg tcactaattc tgtctgttct tcttcatcta atgttctaga gtactctctg
     1021 atattctcaa atactctctc aatttctatc tcagtatagg taatatttaa cgcattattt
     1081 tgtccaccaa tctgtgagtt tatcaaatct ccattaacag aaacatgagt actattatct
     1141 tgatgatatt cgttaaagtt tagtgctgtt ttagaggaat ctagttgtgt tggaaggata
     1201 acaggtttta tcttcttatt agctctcttc accattttat tcgtttctct gactaaattt
     1261 ctctcaatat ctctgaattg actttgcaaa ctttttgcca tttacaccta ctttctatac
     1321 ttcaaaatct ctaactgttt gtttttcttc ttcagccaag tcaaacaagt ccatgacaag
     1381 ttcatcaatt tccagattaa gctgattgac tttttcagtg tcaggagaat cttttttcat
     1441 ctcctccatc agttgctcaa caacttgtgc tatgtcttct tgctgggaat cagtagcatc
     1501 tggaattgga aaatctccta gttcattaac tttgaactgt gggaataggc gacgttggaa
     1561 tttatcaaac ttcatgaaaa accaaagaga gattaagcga gagttcaaaa tacccaaaag
     1621 ataaaaagga ttaacctgaa tatctgtgat gaccatggag tttaagtcgt taataacatc
     1681 actatcagta taaacagctt caacagcata agttgattta cttggaatct gacgaactaa
     1741 aattcgtggt ttttcaaaaa tttctttatc tcttggtgca gctaaatttt caccatactt
     1801 gacatattca ccattccaag tcagtttata gcgtgataca ttctcgccat caagataggg
     1861 acggtatgaa tcatctgacc tttctcttgc atgataaatt cgattattct tatcttgagc
     1921 ggtcatttta ggattccctt ttccagtttc ataggctttg attccagatt ttacagtcga
     1981 aatttctggt cgtcctagta ttggaaaatg gtttactttc cagaagatat caatcgcctg
     2041 acgatacttg accatcgaga ttgaaaaggt agggatttca ccaaagaaat ctggcgcaac
     2101 tcgtccaaca gtgttgaact cgccgtgata caattctgct acttcaatcc agtttggcgc
     2161 attcttcttg aagaaaatga tacagttatc cacgctagca tccgcaaaaa tcttatccag
     2221 agaattaatc agaacaacat cactcgtctg ctccgtgata aactgacgaa ctcttgaatt
     2281 ggtctgaatg gtcagaaaat tatttggaat gatataagag aatgtcccac ctttacgtaa
     2341 gagttgataa gccaattcca tgaatatagt ataggtattg gcttggtatt cacttacttg
     2401 ataagtcctt gaatagtagg atttcatctc atctgtaaag gactgattac gagcaaagat
     2461 ataaggcgga ttcccaatta caaggtcaaa gccgataaat tcgccagctt ctcccaagat
     2521 ctcaggaaat tccattcgcc attccatacc tttagcgaat aatggatttt tcagtctgta
     2581 ttccaattcc ttttcagctt ttgctagttg cttatttaat tccgctactt ctttagaatt
     2641 tttattttca tcctcaaata agctaatggc accagcctta tttcgttcag atatgagccg
     2701 agataatttc tttaattcag ggcttgaagc tgtgtcatca aaggaatgtt tgagcctttc
     2761 gattttctcc cagatttctg ctttaactcg tttattattc gtctccttat agtctttaac
     2821 tagagataga tagtccttga aatctgtttt tctcatatca aattcatagt caaattcaaa
     2881 cttatgtaag agactatcgc ctactttaat actgatatca atatttggca aagtcgtcaa
     2941 aacaggttgg ttggtatcag tatcctggta gtaataagaa gattttaaga gttcaatcca
     3001 aagacgcaaa cggcaaatat tgaccgagct tgggttaata tccactgcaa agagcgaatt
     3061 ttccagaata tgccttttct ggttgaaaat tgccttttga atttgttctg actgctgatt
     3121 acctgtctga tacaaaaaat tatttccaga catatcttga atgactagct catcattgat
     3181 aacataacat tgaatcaggt ttccaatata gttgccatca gcgtcaaaca aaatatttaa
     3241 atcactttta agagcaataa tttcattcag gattgaaaca aggaagtgac cggatccaac
     3301 cgctgggtca caaaccttaa gatcatcaat tgcttggctg actttacgag cattttcaac
     3361 actatgtcca atcgcaaact taatctcttc aacagtttca caattccaac caagcaattc
     3421 attaactttg tccactgctg ctgtacgaat cgctttacgt gacatataca ttgtaatgtt
     3481 acctggcgta taaaaagagc catcacggta gccgttgatt ttctcaaaaa ttaatcccag
     3541 aactgacgca ttgataaggt cattcttaga tttttcatgg tggctgatgg aggtcgagaa
     3601 gtcataagaa tccaaaaact caaagagata ttcaataaaa ttgatgttcc cttttttacg
     3661 ttttttatct acgcctttaa gggctgtttt ggagaaaatt tcaatatctc cctctggtaa
     3721 tctatcaata ccaatcccat ctctagaaat ttctatttct gtttcttcaa acagtgatga
     3781 gttcagataa gggacataac cgaacttttc ttgcactcga tcatttcttt ccgatacttt
     3841 cttagcaagt acagcaaaga atagaccgta aatttcttca aaattaggga gtttttcata
     3901 cgtcaagaac ctataagatt catctttgtt aaagagtaca agctgagatt ctaacagttt
     3961 caaaaataga attctatttg tccagacaac tgttagttga atagcaatat cttcctgctt
     4021 ttcttttgaa acatctttca tctccaattt attaatgata ttctcaacaa aagaatatcg
     4081 ctgacgtttg atgggtttta agcgtgaaat aatttttgaa gtccctgact tcgtttcttc
     4141 cagccccatc aaataaagca attcatcata gaaattctta ttgagtttgt ttgaatcagt
     4201 aaaaatctct ttattgagga gattctctgc tgtgaagaat cgataaagct gagtcagatt
     4261 atttttcttg atttcaatag acttactggt cttaactaga gcttgactaa gatcaaagtg
     4321 agcaataacg attccctttt caaatgcttt gtcaatttct ggtgcaataa cctcactata
     4381 aaggaaatct gttttattgc tagaaagttg attatttctg aactttgtca cgaggtcaac
     4441 tagcttttta tttttgaaaa aatgcttttc aaattcttta gcctctatta caaaccaaga
     4501 tagaccgttt gtgataatac actttttgat ttctaaattt ttattaaata ggcgttcttg
     4561 taggtaataa tagacaattt cttgaaatgc tttagaatta attttttccg ttgacatcat
     4621 ttcagattta ttgaaaagac tcttgcattc aaaaagaata cctagtgacg aattggcatc
     4681 tttaccatta tagatagcaa ggtcaatcct actagaagta tttataaaat tgtaaggtaa
     4741 tacactttct aaaaatacct tgagaagatt tttctggtat tcctctgact catcctcttt
     4801 gtcttttaaa tgagtcaaat aactaaccaa tttttgatta aatacctctt tttcttcatc
     4861 aagtggcata attgcttgaa cagagcgatt catactctct tttaatgtaa ttccctttat
     4921 tgccatgaaa tagactccta aacagattta tttaagaact gatagtatta gtctattgtg
     4981 taagcaatag actaagggta cccttagcga tatatataca tattatatca taaataaaac
     5041 cccaccaaga tttaatcttg gtggggtttt ggtggggaat gataacattc tcccgatttt
     5101 ttatcgctct aagcaattct aaaaccttga tttactaggc ttccgcaatc aaattgcgat
     5161 actctattat ttaagagtaa ctgaagcacc tgcagcttca agtttttctt tgatttcgtt
     5221 agcttctgct tctgaagcac cttctttaat aactgatggt gcgttgtcaa caatagcttt
     5281 agcttcttta agaccttcgc ctgtgatttc acgaacaact ttgataacgc caactttttt
     5341 gtctccagca gctgttaatt caacgtcaaa tgagtcttta gcagcagcag cttcaccact
     5401 agcagcggca gcagctacag gagcagcagc agttacacca aattcttctt cgatagcttt
     5461 tacaagatcg ttaagttcaa gaattgtagc ttctttaatt tcagcaataa tattttcaat
     5521 gtttaatgcc attgttattt cctccaaaaa tatgttttaa tataaaatta tgtagctagg
     5581 ctacttgaag agtgcttatg ccacttcttc gtctttttct gcaactgctt tgacagcata
     5641 tgcaacgttg cgtactggcg cttgaagtac tgaaaggagc attgaaagca taccttcttt
     5701 gtttggtaat ttagcaagag cgttaatttc ttcaactgaa gtgaatttac cgtctactga
     5761 accacctttg atttcaagag cttctgcatc tttagcgaaa tcgctaataa ctttagctgg
     5821 tgcaataaca tcttcgttag aaaacgcaac tgctgatgga ccaacaaaaa gttctttaag
     5881 gtcttcaaga cctgcttttt cagctgcacg aattaaaatt gagtttttga taactttaaa
     5941 ctcaacatcg ctttcacgaa gtgaacgacg taaattagta tcttgctcaa cagtaagacc
     6001 acgtgaatca acaacaacga tacttgctgc tgctttcatt ttctcagcaa taagttcaac
     6061 ttgttcagct ttttttgcaa taattgcttc actcattagt tttacctccg tttttatttt
     6121 ttgggatagg cacaaaaaaa tcgcacctaa acctagacac gaaagtacaa tacgttattt
     6181 tataatgacg ttttgtgcct cggtaggaat attatgagca ctgctcccct actgtcttag
     6241 gtcagtttca tcagaaactt taaatagtat aacttataat tctagcattg tcaataattt
     6301 ttattatttt tctgaaataa ttttatagta ccaaaaaata ctaaaatagt tatcttttat
     6361 atcaaaaaat taatatttta gtatttattt attgaccttc gctgaccaaa ggtgtattat
     6421 agtaccagaa a
//
LOCUS       DATRNK010000082         6351 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.082, whole genome shotgun sequence.
ACCESSION   DATRNK010000082 DATRNK010000000
VERSION     DATRNK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.082"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..199)
                     /locus_tag="VBC52_001834"
     CDS             complement(<1..199)
                     /locus_tag="VBC52_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEO6883393.1"
                     /translation="MNLLFSIDDMYVDHFKVMLYSLVRQTKNRKLEIYVLQKTLLKRH
                     TELIQYTQNLEVGYHPIIVGTE"
     gene            complement(287..1003)
                     /locus_tag="VBC52_001835"
     CDS             complement(287..1003)
                     /locus_tag="VBC52_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEO6883394.1"
                     /translation="MVGLGTVINVILIIVGGFVGLFLKNFLKESLQKSLMQAMGVAVL
                     FISISGVLEKMMLVEKSHLISNHTNMMIITLALGTVLGELLSLDSYIDKFGNYLKQKT
                     GSGNDIKFVEAFVTSTCTVCIGAMAVVGSIQDGIAADHSILFAKGMLDMIIIAIMTVS
                     LGKGALFSALPVALLQGSLTIVAFFMGSLLNPSSLDYLNLVGNMLIFCVGVNLLFNLN
                     IKVINMLPAIILAILWGSFI"
     gene            complement(1085..3703)
                     /gene="alaS"
                     /locus_tag="VBC52_001836"
     CDS             complement(1085..3703)
                     /gene="alaS"
                     /locus_tag="VBC52_001836"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEO6883395.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHSVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPEWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLTQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKAAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFDYGADSLESNLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DHGVIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NDLTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDAAAKVQALS
                     DSLRDLQKENVELKEKAAAAAAGDVFKDIQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKGLAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAENL"
     gene            complement(3719..4204)
                     /locus_tag="VBC52_001837"
     CDS             complement(3719..4204)
                     /locus_tag="VBC52_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEO6883396.1"
                     /translation="MATFQIKEKMFSLGGKFTITDQTGLPCYHVEGSLFPLPKTFKVF
                     DEEGHLISQIEKKVLSFLPKFNVTLANGNHFTIKKDFSFLKPHYTIEDLDMEVKGNFW
                     DMDFQLLKDNQVIANISQQWFRMTSTYQVEVYNETYNDLTISLVIAIDYVKELEKNAS
                     N"
     gene            complement(4446..5375)
                     /gene="prsA"
                     /locus_tag="VBC52_001838"
     CDS             complement(4446..5375)
                     /gene="prsA"
                     /locus_tag="VBC52_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEO6883397.1"
                     /translation="MKTRSKLAAGFLTLMSVATLAACSGKTSNGTNVVTMKGDTITVS
                     DFYDQVKTSKAAQQSMLTLILSRVFDTQYGDKVSDKKVSEAYNKTAKGYGNSFSSALS
                     QAGLTPEGYKQQIRTTMLVEYAVKEAAKKELTEANYKEAYKNYTPETSVQVIKLDAED
                     KAKSVLKDVKADGADFAKIAKEKTTATDKKVEYKFDSAGTTLPKEVMSAAFKLDKNGV
                     SDVVSTVDSTTYKTSYYIIKVTDKTEKKSDWKSYKNRLKEVILKDKTSDRAFQNKVIS
                     KALEKANVKIKDKAFAGILSQYATTSGSSSLKK"
     gene            complement(5436..6143)
                     /locus_tag="VBC52_001839"
     CDS             complement(5436..6143)
                     /locus_tag="VBC52_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEO6883398.1"
                     /translation="MVQSYSKNANHNMRRPVVKEEIVQYMRQHQKQNNGCLAELEAFA
                     KQENIPIIPHETATYFRFLMQTLQPKHILEIGTAIGFSALLMAENAPEAKITTIDRNE
                     EMIALAKENFAKYDNHNQITLLEGDAVDVLQTLDKSYDFVFMDSAKSKYIVFLPQVLK
                     HLDVGGVVVLDDIFQGGDIAKPIDEVRRGQRTIYRGLQRLFDSTLQHPDLTATLVPLG
                     DGLLMIRKNADHIVLED"
     gene            complement(6217..>6351)
                     /locus_tag="VBC52_001840"
     CDS             complement(6217..>6351)
                     /locus_tag="VBC52_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6883399.1"
                     /translation="RPLDLEVAQNAGIKSINLRLENSKENYNISSLKDIISLDFTRLD
                     "
BASE COUNT         1886 a         1291 c         1073 g         2101 t
ORIGIN      
        1 cttcggtacc tacaattatt ggatgatagc caacttctaa attttgagta tattgaataa
       61 gttctgtatg acgtttgagt aatgtttttt gtaagacata tatctctaac ttacgattct
      121 tggtttgtcg gacaagcgaa taaagcatca ctttaaaatg atctacgtac atatcatcaa
      181 tagaaaataa tagattcatg agttggatcc tctccgtagt atatcttctt tcatcctaac
      241 atatgtctaa agaaaaacca agtttgagtt aaaacttggt tatcttttat ataaaacttc
      301 cccataatat agctaaaata attgctggca acatattgat aactttaata tttaggttaa
      361 agagtaaatt aacaccgaca cagaatataa gcatatttcc tactaagtta aggtaatcta
      421 aagatgatgg attaagtaga ctccccatga aaaaagcaac aatcgtaagg ctaccttgta
      481 gtaaagcgac aggaagtgca gaaaatagag ctcctttccc taaagacact gtcataattg
      541 caataataat catgtctaac attcccttag caaataaaat cgaatgatct gctgctatac
      601 catcttgaat gctaccaaca acggccattg ctccaataca aacggtacaa gttgaggtga
      661 cgaatgcctc cacaaattta atatcgttac cacttcctgt tttttgctta agatagttcc
      721 caaatttatc aatatacgaa tctagtgata gcaactctcc aagtaccgta cctagagcaa
      781 gagtaataat catcatattt gtatgattag agatgagatg tgacttttcg actaacatca
      841 ttttctccaa aacacctgat atcgaaataa ataaaactgc aactcccatg gcttgcataa
      901 ggctcttttg aagtgattct ttcaaaaaat ttttcaaaaa taaacctaca aagcctccaa
      961 ctataataag aataacattg ataactgttc ctaaaccaac catatttctc ctatatacac
     1021 aaaggaacag aacgatgttc tgccctttgt ctgttttatt tactaactaa atgagaggtg
     1081 tttcttataa attttctgca actgctgcta gcaactctgc aattttactt gcatcgctac
     1141 cacctgccat agccatgtct ggtttaccac caccacgacc tgctacaatt ggtgccaatc
     1201 ctttgatcat gttaccagcg tggacatctt tagttttgct tgcaacaagg acattaacct
     1261 tctcaccaat agctgctacg agaacaagca catcagagta gtctttttgt ttccagttat
     1321 cagcaaatgt acgaagtgca cctgcgtctg caacatcaac ttgactagca atgaagcgca
     1381 cgcccttagc ttcttgaata tctttaaaga catcaccagc tgctgcagct gctgcttttt
     1441 ctttaagctc tacattttct ttttgaagat cacgaagtga gtcgctaaga gcttgtactt
     1501 tagctgctgc atctttcaat tgcggagctt ttacagtagc agcgatctct tttagtgcat
     1561 cctcttggtt acgataagct tcaaaagctt gtctaccagt aactgcaata atacgacgag
     1621 tacctgaacc aataccttct tctttgacaa tcttgaagag accgatttct gaagaattat
     1681 ttaagtgagt tccaccgcaa agttcaacag aataattacc aatttgaacc acacgaacca
     1741 ctttaccata tttctcacca aaaagtgcca ttgctcccat ctcttttgcg gtttcaacgt
     1801 cagtttcagt cgttgtgatc gtaagatcgt tccaaatttg ctcattaact tcttgttcaa
     1861 tgtgacgaag ttcctcattg cttactgctt caaagtgagt aaaatcaaag cgcaagaatt
     1921 cttcttcgtt caatgaacca gcctgagttg cgtgttcacc gataacattg tgaagagctg
     1981 catggagcaa gtgagttgct gtgtggtttt tctcaacagc caaacgacgc tctttgttga
     2041 tttcaagtgt gtagtttgtt ccaactgaaa gtgatgccaa aacgtttaca gtgtgtagag
     2101 gttgaccatt tggtgctttt tgaacatcaa caacctcagc aactgtgtca cccttatcat
     2161 ttttgatgac accgtggtca gcaacctgtc cacccatttc agcatagaat ggtgtttgag
     2221 caaagacaag aagagcttga ccttctgaaa ctgcttcagt acgttcattg tcagcgatga
     2281 tgactgaaag atttgattca aggctgtcag ctccgtagtc gaagcgtgat tcttcaacaa
     2341 taccagctag ggtttcattt tgcataccca ttgaaccccc cttaacaaca gctgcacgcg
     2401 cacggtcttg ttgttctttc atggctgctt tgaatccttc gtggtcaatc ttgtagcctg
     2461 catcttctgc caattcctct gtcaattcaa ccgggaatcc ataagtatca taaagtttga
     2521 agatatcttt accttcaagt gtatctttac cttcagcttt aagctgcgta agcaatgaat
     2581 ccaagtgacc gctacctgca tcgatagtac gagcaaatgt ttcttcctca cgtttaacga
     2641 ttttctcgat aaaatcacgt ttttcaagca cttctgggta gtagctttcc atgatttgtc
     2701 caacagtcgg aaccaatttg taaaggaaag tttcgttgat gccaagacgg cgaccgtgca
     2761 taaccgcacg acgaagaaga cgacgaagaa cgtaaccacg accttcattt ccaggaagcg
     2821 caccatcacc gatagcaaat gaaagcgcac ggatgtggtc agcgataacc ttgaaactca
     2881 tgttgtcgcc atctggatcg taagttttac ctgacaattt ttctacttca cggatgatag
     2941 gcatgaaaag gtcagtttca aagtttgttt ttgccccttg cataacagct gcaagacgtt
     3001 caagaccagc acccgtatca atgtttttgt ttggcaattc tttgtattct gaacgtggca
     3061 cagcaggatc agcattgaat tgtgagagaa cgatgttcca gatttcgatg taacgatcgt
     3121 tttcgatatc ttcagccaaa aggcgaagtc cgatattttc tggatcaaaa tcttcaccac
     3181 ggtcgaagaa aatctcagta tctggacctg aaggaccagc accgatttcc cagaagttat
     3241 cctcgattgg caccaagtga cttggttcaa cgccacaagc aatccaacgg ttatatgaat
     3301 ccttgtcatc tgggtaataa gtcatgtaga gcttgtcttt agggaagtca aaccattcag
     3361 gacttgtcaa gagttcaaat ccccactcaa tagcttcatc acggaaatag tctccaattg
     3421 agaagttacc aagcatttca aacatagtat ggtgacgtgc tgttttacca acattttcaa
     3481 tatcattagt acgaattgat ttttgtgcat tggtaatacg tggattttct ggaatcactg
     3541 aaccatcaaa atattttttc aaggttgcaa cacctgagtt gatccaaaga agcgttgggt
     3601 cgttcacagg aaccaagtta gctgaaggct caacggaatg tcctttggat ttccagaaat
     3661 ccaaccacat ttggcggatt tgtgcagacg ataattcttt cattattctc tcctaaaatt
     3721 agtttgatgc atttttttct aactctttga catagtcgat cgctatgaca agcgaaattg
     3781 tcaaatcgtt gtaagtttca ttgtaaactt ctacctgata agttgaagtc atacgaaacc
     3841 actgttgaga tatgtttgct ataacttgat tgtcttttaa gagttggaaa tccatatccc
     3901 aaaaatttcc cttaacttcc atatcaaggt cttcaatggt gtagtgaggt ttcaaaaatg
     3961 agaaatcttt cttaatcgtg aaatgattcc cattggctaa tgtcacatta aactttggta
     4021 aaaaacttaa aaccttcttc tcaatctgac taatgagatg cccctcttcg tcaaagactt
     4081 taaaagtctt aggtagaggg aacaaacttc cttcaacgtg ataacaggga agcccagtct
     4141 ggtctgtaat agtaaattta ccgcctagag aaaacatttt ttctttaatt tgaaaagtag
     4201 ccatatctct tcctcctcga aagttgacaa atagcaaaag acaagtccat tacgaagggc
     4261 gaaaaccgcg gtaccacctt cattcaatga acttgtcatt ctcatttatt tttagtttta
     4321 ccccattgag tagcatcttt ttctgattat ccgtgactcg cagcaaccgt cacttttctg
     4381 tatctaatcg ttgaaatttc attctcaata gtattaatta tacagtcttt ttaagacttg
     4441 gtcaactatt ttttaagtga tgatgaacca cttgttgtag catattgtga taagatgccc
     4501 gcaaatgctt tatctttaat cttaacatta gctttttcta aggcttttga aatcacttta
     4561 ttttggaagg ctctatcgct tgttttatcc ttaagaataa cttcttttaa gcgatttttg
     4621 taagatttcc aatcagattt tttctctgtc ttatcagtta ctttaatgat gtagtaactt
     4681 gttttataag ttgttgaatc aaccgttgaa accacatctg aaacaccatt tttatctagc
     4741 ttaaaggctg ctgacataac ttctttaggg agggttgtcc ctgcagaatc aaatttatac
     4801 tcaactttct tatcagtagc tgttgttttt tcttttgcaa tctttgcaaa atcagctcca
     4861 tcagccttta catctttaag gacagattta gctttatctt ctgcatccaa tttgattact
     4921 tgtacagaag tttcaggagt atagttttta tatgcttctt tatagtttgc ttctgttaat
     4981 tctttcttag ctgcttcttt tacagcatat tccacaagca tagttgtgcg aatttgttgt
     5041 ttgtaacctt ccggagtcaa acctgcttgt gaaagtgcgc ttgaaaatga attaccatag
     5101 cctttagctg tcttattata agcttctgat acttttttat ctgaaacttt atcaccatac
     5161 tgtgtatcaa aaacacgtga gaggatcaat gtaagcattg attgttgtgc agcttttgat
     5221 gtttttactt gatcataaaa atcagagact gtaatagtgt cgcccttcat tgtaacaaca
     5281 tttgttccat ttgatgtttt acctgagcag gcagctaatg tggcaactga cataagtgtc
     5341 aaaaagcctg ctgcaagttt tgatcttgtt ttcatgattt ataactcctt tttgtcatta
     5401 actccattat tatatcataa tttcttaaaa atgccttaat cttctagtac aatatgatct
     5461 gcatttttac gaatcatcaa taaaccatct cccaaaggta ccaaagtagc agttaaatca
     5521 ggatgttgca aagtcgagtc aaataaacgt tgcagacctc ggtagatagt tcgctgacct
     5581 cttctcactt catcgatagg ttttgcaata tctccacctt ggaaaatatc atctaaaaca
     5641 actactcctc caacatctaa atgttttaat acttgaggta aaaaaacgat atatttagac
     5701 ttagcagaat ccatgaatac aaaatcgtaa gatttatcca atgtttgtaa cacatccaca
     5761 gcatcgcctt ccaaaagggt tatctgattg tgattatcat attttgcaaa gttttcctta
     5821 gccaaggcaa tcatctcttc attacgatcg atcgttgtta tctttgcttc aggagcgttt
     5881 tctgccatca aaagggccga aaaaccaata gctgttccaa tttctaaaat gtgcttaggt
     5941 tgtaatgttt gcatcaaaaa acgaaaataa gtcgctgttt catgaggtat aatagggata
     6001 ttttcctgtt tagcaaatgc ttctaactct gctaaacagc cattattttg tttttggtgc
     6061 tgacgcatat attgaacaat ttcttcttta actactggac gacgcatatt atgattagca
     6121 tttttactgt aagattgaac cacttttggc tcctcttttc tattttaaat aataactttt
     6181 ttattatagc aaaaaatgtc taactctgag aatgatttaa tccaaacgag tgaaatcaag
     6241 tgatattata tctttgagac ttgaaatatt atagttttct ttggaattct ctaaccttaa
     6301 gtttatggat tttataccag cattttgagc aacctccaaa tctagtggac g
//
LOCUS       DATRNK010000083         6296 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.083, whole genome shotgun sequence.
ACCESSION   DATRNK010000083 DATRNK010000000
VERSION     DATRNK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.083"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(442..2418)
                     /gene="ftsH"
                     /locus_tag="VBC52_001841"
     CDS             complement(442..2418)
                     /gene="ftsH"
                     /locus_tag="VBC52_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEO6883400.1"
                     /translation="MKNNKNNGFLKNSFIYILLIIAVITTFQYYLKGSSSQNQQISYT
                     KLVKQLKAGEIKSISYQPSGGVVEVSGTYKKAKTIKSANSFTFLGGSVATKVTGFNSV
                     ILPNDSSIKSLVSAAEENNTNIQVKHESSSGTWISYIASFLPLVIMIGFFMMMMNQGG
                     GGGARGAMSFGKNKARSSSKDEVKVRFSDVAGAEEEKQELIEVVDFLKDPKRYKSLGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDA
                     KKAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNESIIVIAATN
                     RSDVLDPALLRPGRFDRKVLVGQPDVKGREAILRVHAKNKPLADNVDLKVVAQQTPGF
                     VGADLENVLNEAALVAARRNKKVIDASDIDEAEDRVIAGPSKKDRTISDRERAMVAYH
                     EAGHTIVGLILSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKDDMKEQLAGLMGG
                     RVAEEIIFNAQTTGASNDFEQATAMARAMVTEYGMSEKLGPVQYEGNHAMMAGQMSPE
                     KSYSAQTAQLIDDEVRHLLNEARNKAADIINENRDTHKLIAEALLKYETLDAAQIKSI
                     FETGKMPETENDEDKARALSYDEIKEKMQEEDKK"
     gene            complement(2441..2983)
                     /gene="hpt"
                     /locus_tag="VBC52_001842"
     CDS             complement(2441..2983)
                     /gene="hpt"
                     /locus_tag="VBC52_001842"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEO6883401.1"
                     /translation="MLENDIKKVLYSEEDIILKTKELGAKLTADYAGKNPLLVGVLKG
                     SVPFMAELLKHIDTHVEIDFMVVSSYHGGTTSSGEVKILKDVDTNIEGRDVIFIEDII
                     DTGRTLKYLRDMFKYRQANSVKVATLFDKPEGRLVDIDADYVCYDIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEIYSK"
     gene            complement(2988..4262)
                     /gene="tilS"
                     /locus_tag="VBC52_001843"
     CDS             complement(2988..4262)
                     /gene="tilS"
                     /locus_tag="VBC52_001843"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEO6883402.1"
                     /translation="MYNTILKDTLSKGLFTAHQKVLIAVSGGIDSINLLQFLYQYQKE
                     LSISIGIAHINHGQRKESEKEEEYIRQWGQIHDVPVFISYFQGIFSEDRARNHRYNFF
                     SKVMREEGYTALVTAHHADDQAETVFMRILRGSRLRYLSGIKQVSAFANGQLIRPFLP
                     YKKELLPNIFHFEDASNASSDYLRNRIRNVYFPALERENNQLKDSLITLSEETECLFT
                     ALTDLTRSIEVTNCYDFLRQTHSVQEFLLQDYISKFPDLQVSKEQFRVILKLIRTKAN
                     IDYTIKSGYFLHKDYESFHITKIHPKTDSFKVEKRLELHNIQIFSQYLFSYGKFISQA
                     DITIPIYDTSPIILRRRKEGDRIFLGNHTKKIRRLFIDEKITLKDREEAVIGEQNKEL
                     IFVIVAGRTYLRKPSEHDIMKDKLYIENLEKR"
     gene            complement(4264..5550)
                     /locus_tag="VBC52_001844"
     CDS             complement(4264..5550)
                     /locus_tag="VBC52_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6883403.1"
                     /translation="MKKVLTFLLCSLYFVSIPAISTEEPLTLSQNRRYALTQTVVDKE
                     MYFDTIPERPTTKIEISSFQDEALTITGETLVPNTLLSIVSLTINSNGIPVFTLSNGQ
                     FIKASREAIFNDLVSKQQSVSLDYWLKPSFVTYEAPYTNGVSEVKNNLKPYSRVHLVE
                     QAETEHGIYYKTDSGFWISVEDLSVADNRMAKVQEVLLEKYNKDKYGIYIKQLNTQTV
                     AGINIDRSMYSASIAKLATLYASQEQVKLGKLSLDSKFEYKDNVNQFPNSYAPSGSGK
                     LEKKADHKLYTVKELLEATAKESDNVATNMLGYYVNNQYDSMFQTQVDTISGMHWDMK
                     KRQISPQAAGKMMEAIYYQNGDIVNYLSKTDFDNTRIPKNIPVKVAHKIGDAYDYKHD
                     AAIVYAEQPFIMIIFTDKSSYDDITKIADDVYQVLK"
     gene            complement(5550..5684)
                     /locus_tag="VBC52_001845"
     CDS             complement(5550..5684)
                     /locus_tag="VBC52_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEO6883404.1"
                     /translation="MNNLIETIEHFLAYSDDKLEELADKNYQLRQIIVEKEKIESREK
                     "
     gene            complement(5687..6058)
                     /locus_tag="VBC52_001846"
     CDS             complement(5687..6058)
                     /locus_tag="VBC52_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEO6883405.1"
                     /translation="MSKPNVVQLNNQYINDENLKKRYEAEELRRKNRLMGWVLIFVML
                     LFILPTYNLVKSYRTLQERRQEVVKLTKDYQTLTNRTENQKLLAKQLKNPDYVQKYAR
                     AKYYFSKTGEMIYPLPDLLPK"
     gene            complement(6045..>6296)
                     /locus_tag="VBC52_001847"
     CDS             complement(6045..>6296)
                     /locus_tag="VBC52_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEO6883406.1"
                     /translation="KVSRIIKRRPVAKEVADKGRVKVNGVLAKSSTDLKLNDQVEIRF
                     GNKLLTVKVLEMKDSTKKEDAIKMYEIINETRIETDEQA"
BASE COUNT         1896 a         1205 c          918 g         2277 t
ORIGIN      
        1 gtcattgacg gatttacaat gtaaatccac cctgcacatt cgttcgtctt gttcagtttt
       61 caaaggtctt tgcctctctt gagacaactt ctatattcta gcaaacttat tctgctttgt
      121 caactacttt ttttaagttg ttaactacgc gctaatagaa actgctctct cgagacaact
      181 tattcattat actaaatatt tctacttcct gtcaatacta tttttgcatt ttttctttta
      241 tttttaaaaa gttaatatta tttatagtaa ctaaccttct atacttgttt aatggatagc
      301 atttttaccg ttgtcatgtt catattttat cttcttaatt cacaaattta aacttcatct
      361 tcataaaaaa tacccctcaa attttatcta aatttgaggg gtgtttgaaa tttataaagt
      421 tctttaaaaa tatatgatga cttatttttt atcttcttct tgcatttttt ctttgatttc
      481 atcgtatgat aacgctcttg ctttatcttc atcattttct gtctcaggca ttttacctgt
      541 ctcgaaaatc gacttaatct gagcagcatc aagagtctca tattttaaga gggcttctgc
      601 aattaattta tgagtatcac ggttttcgtt gataatatca gctgccttat tacgtgcttc
      661 attaagaagg tgacgaactt catcatcaat aagttgtgca gtttgagcag aatatgattt
      721 ttcaggtgac atttggccag ccatcattgc gtggttacct tcgtattgaa ctggtccaag
      781 tttctcgctc ataccatatt cagttaccat agcgcgggcc atggcagtgg cttgttcgaa
      841 gtcatttgag gcacctgttg tctgagcgtt gaaaataatt tcttccgcta cacgtcctcc
      901 cataagacct gctaattgct ctttcatatc atcttttgaa agaagcattt gatcttcttt
      961 aggtaaagca atcatatatc cacctgcacg accacgtggt acgatagtaa ctttatgaac
     1021 aactcgcgca tttgaaagaa tcaaaccgac aattgtgtgc ccagcttcat ggtaagcaac
     1081 catagctctt tctctgtcag aaatagtacg atctttttta gaaggaccag caattacacg
     1141 gtcttctgct tcatcaatat ctgaagcatc aataactttt ttatttcgtc gcgcagcaac
     1201 taaagcagct tcattgagaa cattctccaa atcagcacca acaaatcctg gggtttgttg
     1261 agctactact tttaagtcaa cattatctgc taatggttta tttttagcat gtactctcag
     1321 aattgcttca cgacctttta catcaggttg tccaactaga acttttctat caaaacgtcc
     1381 tggacgcagt aatgcgggat ctaacacgtc actacggttg gtagcagcga taacgataat
     1441 actttcgttt ccttcaaaac catccatttc aattaaaagt tggttgagcg tttgttcacg
     1501 ttcatcgttt ccgccaccca taccagctcc acgtcgacgt ccaacagcat caatttcatc
     1561 aatgaaaata atagcacgtt cagctttttt agcgtcttca aacaaagagc gtactcgact
     1621 agctccaaca ccgacaaaca tttcaacaaa atctgaaccg gaaatactaa agaatggaac
     1681 ccctgcttca ccggcaacag cttttgctaa tagggtttta cctgtccctg gaggtccttc
     1741 taatagaacc cctgctggaa ttcgggcacc tagagattta tatcgtttag gatctttaag
     1801 gaagtcaacg acttctatta attcttgctt ttcttcctct gctccagcaa catccgaaaa
     1861 tcgaacttta acctcatctt tactagatga tcttgcttta tttttaccaa agctcatggc
     1921 accacgagcg ccaccgccac caccttggtt catcatcatc ataaagaaac caatcatgat
     1981 aactaaaggt aaaaaactgg caatataaga aatccatgtc ccacttgaac tttcatgttt
     2041 gacctgaata ttagtgttat tttcttcagc agcgctaaca agagatttta ttgaagagtc
     2101 atttggtagt ataacagaat taaatcctgt aactttagta gcaacactac cacctaaaaa
     2161 agtaaaacta ttagctgatt taattgtttt ggctttctta tatgtcccac taacttcaac
     2221 aacaccacca cttggctggt atgaaataga cttaatttct ccagctttta attgtttaac
     2281 aagttttgta taagaaattt gctggttttg agagctagat ccttttaaat agtattgaaa
     2341 ggttgtaata accgcaataa tcaataatat gtaaataaag gaatttttca gaaaaccatt
     2401 atttttatta tttttcatag tttaagagag tacctttttt ctattttgag taaatttctt
     2461 cttttaacac accgacataa ggtaggttac gatagttttc agcatagtct aaaccgaatc
     2521 ccacaataaa ttcatttggg atatcgtaac aaacgtaatc agcatcaata tctaccaatc
     2581 gcccttctgg cttatcaaaa agggttgcaa ctttgacaga gttagcttga cgatacttaa
     2641 acatatctcg aaggtatttt aatgtacgtc cagtatcaat gatgtcttca ataaagatta
     2701 catctcttcc ttcaatatta gtatcgacat cttttaaaat tttaacttct ccactactag
     2761 ttgtaccacc atgatagctt gaaacaacca taaaatcaat ttcaacatgt gtatcaatat
     2821 gctttaatag ttcagccata aaaggcactg acccctttaa tacacctact aataatggat
     2881 tcttacctgc ataatctgca gttaatttag ctcctaattc tttcgttttc agaataatat
     2941 cttcttctga atataaaact ttttttatat cattttcaag catgatgtca ccttttttct
     3001 aaattttcaa tataaagttt gtccttcatt atatcatgtt cagaaggttt tctcaaatat
     3061 gttctacctg caacaatcac aaatatcaac tctttatttt gctccccaat aacagcttct
     3121 tctctgtctt ttagagttat tttctcatca ataaatagac ggcgaatttt tttagtgtga
     3181 tttcctaaaa atatcctatc cccttctttt cttcttctta aaataatcgg agaagtatca
     3241 taaataggaa tagtaatgtc tgcttgagaa atgaattttc cataggaaaa taaatattgt
     3301 gagaatattt gtatattatg caattctaat cttttttcaa ccttaaaact atccgtctta
     3361 ggatggattt ttgtaatatg aaaactctcg taatccttat gaagaaaata gcccgattta
     3421 atagtatagt caatattggc tttagttcta atcaatttta atattactct gaactgttct
     3481 ttactcactt gtaaatctgg aaatttgcta atgtaatctt gcaataaaaa ttcttggaca
     3541 gaatgagttt gccttaaaaa atcatagcag tttgttactt cgatgctcct tgttaggtct
     3601 gttaaagccg tgaataaaca ctctgtttcc tcagagagag ttatgagaga atctttcaat
     3661 tgattatttt ctctttccaa agcaggaaag tagacgtttc ttattcgatt tcttaaataa
     3721 tcagacgaag cgttgctagc atcttcaaaa tgaaaaatat ttggtaataa ctccttttta
     3781 tatggaagaa atggtctaat caattgaccg tttgcaaaag cactcacttg cttaatccct
     3841 gataaatagc gtagcctgct tcctcttaaa attctcataa atacagtttc agcttgatca
     3901 tcagcatggt gagctgttac taaagccgtg taaccttctt cacgcataac ttttgaaaaa
     3961 aaattgtagc gatggttacg cgctctatct tctgaaaata ttccttgaaa ataagagata
     4021 aagacaggga catcatgtat ctgtccccac tgtctgatat attcttcttc tttttctgat
     4081 tcttttcgct gtccatgatt tatgtgtgca ataccaatac taatcgataa ctctttttga
     4141 tactggtata aaaattgcaa caaatttatt gaatcaatgc caccagaaac agcaattaga
     4201 actttctgat gagcggtaaa taacccttta gataaggtat ctttcaaaat agtgttatac
     4261 atattatttt aaaacctggt aaacgtcgtc agcaatctta gtgatatcat cgtaagatga
     4321 tttatccgta aagataatca taataaaagg ttgttctgca taaacaatag ccgcatcatg
     4381 tttgtagtca taagcatctc caattttatg tgctacttta actggtatat ttttagggat
     4441 tcttgtatta tcaaaatcag tctttgatag gtaattaaca atatctccat tttgataata
     4501 aattgcttcc atcattttac ccgcagcttg tggagaaatc tggcgtttct tcatatccca
     4561 gtgcatacca gaaattgtgt ctacttgtgt ttgaaacatt gaatcgtatt gattattgac
     4621 ataataacct agcatatttg ttgcaacatt atctgattcc ttggcagttg cttcaagtaa
     4681 ctccttaacg gtatataatt tatgatcagc ttttttctct aatttcccac taccacttgg
     4741 agcatagcta ttaggaaatt gattaacatt atctttgtat tcaaatttgc tgtccaatga
     4801 cagttttcct aattttactt gctcttggct agcataaagt gttgctaatt ttgcaatact
     4861 tgcagagtac attgacctat caatattaat accagctact gtctgtgtat ttaattgttt
     4921 gatgtatata ccatatttat ctttattata tttttccaat aatacttctt gaaccttagc
     4981 catacgatta tcagcaaccg atagatcttc tactgatatc caaaaaccac tgtccgtttt
     5041 ataataaata ccatgttctg tttctgcttg ttctactaaa tgcactctgg aataaggttt
     5101 taaattattt ttaacttcac taacaccatt tgtgtaaggt gcttcataag tcacaaaaga
     5161 aggttttaac caataatcca atgagacaga ttgctgctta gaaactaaat cattaaaaat
     5221 agcttcacga ctagccttta tgaactgtcc attggacaaa gtaaatactg gaattccatt
     5281 tgaattaatt gtaagagaaa caatcgacaa taaagtattt ggaactaaag tctctcctgt
     5341 tatggttaaa gcttcatctt gaaaactgct tatttcaatt tttgtagttg gtctctctgg
     5401 aatagtatca aaatacattt ccttatccac aactgtctga gttaaggcgt atcgtctatt
     5461 ttgagaaagc gttagtggct cctcggtact tatagctggg attgatacaa aatataagct
     5521 acataataag aaggttaaaa ctttcttcat tacttttctc tgctctcaat cttttctttt
     5581 tctacaatta tttgcctaag ttgatagttc ttatcagcta actcttctag tttgtcatcc
     5641 gagtaggcta gaaaatgttc tattgtttca attaaattat tcataattat tttggtaaaa
     5701 ggtctggtaa tgggtaaatc atttcgccag tcttagagaa ataatacttc gctcgagcat
     5761 atttttgaac gtaatctgga ttttttagtt gttttgctag taacttctgg ttctcagttc
     5821 tattagttaa tgtctgatag tctttcgtta attttacaac ttcttgacga cgttcttgta
     5881 aagttctgta actcttaact aaattataag tgggtaaaat aaataaaagc atgacaaaaa
     5941 taagaaccca acccattaaa cgattttttc ggcgtaactc ctcagcttcg taacgttttt
     6001 ttagattctc atcgttaata tattgattat ttaactgaac aacattaggc ttgctcatct
     6061 gtttctatcc ttgtttcatt aataatttca tacatcttga tagcatcttc tttcttagtg
     6121 ctatctttca tttctaatac tttaacggtc aacaatttat taccaaaacg aatttcaact
     6181 tggtcattaa gctttaggtc agttgatgac tttgctaata cgccattcac cttgacacgt
     6241 cctttgtctg caacttcttt agcaacggga cgacgcttaa tgatacgcga cacttt
//
LOCUS       DATRNK010000084         6224 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.084, whole genome shotgun sequence.
ACCESSION   DATRNK010000084 DATRNK010000000
VERSION     DATRNK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.084"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(152..1378)
                     /locus_tag="VBC52_001848"
     CDS             complement(152..1378)
                     /locus_tag="VBC52_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6883407.1"
                     /translation="MAKTRYSGVFIDTKGKYFYETELGIDKISGKRIRKKSRKDSNGK
                     PFSTAREAYLELTRIKREYHKAHAYANYNMNVKQFILDCYIPYYKSYVEESTYAVRIK
                     NLEKIAERFENRTLRNLTIEDIQNYRIWLLSRKEENGSGYSQAYASLIFGMFRKSLDY
                     AVEMGYLENNISKKAKAIPKGKAIVPYWTKVEFERVIAQICIDDFYEHLNFVMLWTYF
                     TTGIRINEGMGLFWNDIDFAKKRMRVHHMLILKSRKNWQRNAYTKTSDGKRIISLDDD
                     TIAILKHWKKRQKEIGLGNETDFVFTYDSFPMIKSTLARIIKRYSELAGVKRIQGKGL
                     RHSNASYLINEFNVSVLILSKRLGHSSPEITLKHYSHLWSGADESIAEIMSGNISIHT
                     APKTKIQFNGNQNLKR"
     gene            complement(1382..1576)
                     /locus_tag="VBC52_001849"
     CDS             complement(1382..1576)
                     /locus_tag="VBC52_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEO6883408.1"
                     /translation="MKPLISKDDLIKLGYKKHTAISIIQAVKQDLVYNKGIPFYNNRR
                     LGLVPRKNVENLIGVELDED"
     gene            complement(1581..2399)
                     /locus_tag="VBC52_001850"
     CDS             complement(1581..2399)
                     /locus_tag="VBC52_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883409.1"
                     /translation="MTREVYNTKIVQYSSNTFEIIHYRNPKIRRLGENGIKDTTPSCK
                     KPSYDELYQQSHRIKRRIRHYLLCNDFDLFWTLTFDDAKINSHDYLIVKKKIRTWLKA
                     QREKYGKFRYIFIPELHKNGRLHFHGVTGGFSPKLTKARNTKTGRLIKKNGKQVYNVD
                     SYQLGFSTVTKIDSSKKVANYITKYITKDLLAIPSGYKQPKYFSSRGLIKPIVSYENL
                     ERNYFEGLTPSFSLGHLDNLEYKEDIAIYNLSIDENGDITANSTGTKIRIRKEL"
     gene            complement(2501..3910)
                     /locus_tag="VBC52_001851"
     CDS             complement(2501..3910)
                     /locus_tag="VBC52_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEO6883410.1"
                     /translation="MILNKIKRNFSRYDFREDSPTYLSISETIEVRKGKQSAILKKLF
                     KVLLLSLPITFLSSMLPILNVVYKIGIIVSSALVALFIGHIGMRLLFSVYSPIWKYRF
                     LSILELATERLDLIQLPSNKTQIKTVEWKFYKEADRIVIKLNPSGTIPSIQQVKEISR
                     RLTEFLIQATHEEWNLLESTIDSNIVVLKYGENPIRYSVSELFEITDDDYCVNHNPIK
                     LYDNIFFDIASEASHQIIIAPSGAGKSLYLATLAGLLIKQGHSVSLIDAKQTSLGATF
                     ENLGIPVARNAEEIVLLLEQMVFEMEDIYKRYFSFSSVDFSTTYKDFSLPAHYLIFDE
                     VLAALESGTPAQQKEMVRLLKILALKSRASGRGLLILASQKLLASDLPRAITEQCQTR
                     IILASDASISEETFYSIMGKEKKDLLVDYRGGVGKGYILTPKTNGIKYFETPYFDLNS
                     RDFKDKIRDFQTIPRKQIE"
     gene            complement(3907..4344)
                     /locus_tag="VBC52_001852"
     CDS             complement(3907..4344)
                     /locus_tag="VBC52_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883411.1"
                     /translation="MSINIENVSFSSPSLKNLLIGTSNVEHIPTTAFVIECRDIPKRN
                     EKNEVIDGTISKKVLSALQPEIVKAIQEVGGDLSTIKPFTIEVQNDEASLKKLNNEAI
                     IAKTIDLDEALLALKWVSDGRNSGAYREFKLIISEVKLLGGKS"
     gene            complement(4521..5183)
                     /locus_tag="VBC52_001853"
     CDS             complement(4521..5183)
                     /locus_tag="VBC52_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883412.1"
                     /translation="MPITKFKNSCHIIFKNCSERIQKVYEDYGYQSNISFYPNDEKLI
                     GNILSYSSLDKLRAEYLITPGVINFLVKQEHYFHDENELLWGDNIDDYLEDFFIAMIL
                     DIQEIPEYAKHLLNLSLLDTNSIKEYFQVNFSFGSSNYDELKDKFIDFTYNQFDTIEI
                     LEEDSIFSFIEKDSVLLSSKNKDTFLTFKYLPDKLELLAKYVLLPIIDKITLENLINK
                     ND"
     gene            complement(5191..5403)
                     /locus_tag="VBC52_001854"
     CDS             complement(5191..5403)
                     /locus_tag="VBC52_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6883413.1"
                     /translation="MISYKPFYETLFNQGKTEYELIFHHGLSSNTLHRMKKGKPITTA
                     TLDTLCFILDCNVSDVITYIPDEGEE"
     gene            5517..>6224
                     /locus_tag="VBC52_001855"
     CDS             5517..>6224
                     /locus_tag="VBC52_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883414.1"
                     /translation="MKLEDFQTYPFKSLQEYISDKDRAVKFSFLLQGDEPDRSELTKL
                     IYSEIFEWYNENEHSGDTLITYRTAIFRKYYGKSYRNLSRDKQNEILNVIKSYTHCDE
                     DFPFEFELEDKENPVYQICNNYQIGNFGIFPKGRINPKRAQSPYNDYFDLALSVIWEL
                     YDGTLTPDDEFKKAILDEKEYFNQFEDFETFLSENFLTVFFDDEGYLIRLSEIDSFEE
                     YVRMSNQIIYTRGIAILN"
BASE COUNT         1973 a         1018 c          984 g         2249 t
ORIGIN      
        1 aagaaataag agtaaaaaaa cttagctact taaagtagct aagtttttat tcccactcaa
       61 ttaaaaattt cataggttga gtgccagaaa accttgattt attaagggtt gtaatgcctt
      121 ttaaatctat ttggcgggat ttctggcggg gttatctttt taggttttga ttcccgttaa
      181 attgaatttt tgtttttgga gccgtatgta tggatatatt cccactcatt atttcagcaa
      241 tagattcatc agctccactc caaaggtgtg agtaatgttt caaagtaatt tctgggctag
      301 aatgacctaa ccttttcgat aaaatcaaga cagatacatt aaattcgttt atcaaataag
      361 aagcgttact atggcgaagc cctttacctt gtattcgctt tacgccagca agttcagagt
      421 atcgtttaat aattctagct agtgtagatt ttatcatcgg aaagctatca taggtaaaga
      481 caaaatcagt ttcattacct aagccgattt ctttttgtct tttcttccaa tgctttagaa
      541 tagcaatagt atcatcatct agggaaataa ttcgtttgcc atcacttgtt ttagtgtagg
      601 cgtttctttg ccagtttttc cttgatttta gaataagcat atgatgtaca cgcattcgtt
      661 tcttagcgaa gtcaatatcg ttccagaaca gccccatgcc ttcgttgatg cgtatgcctg
      721 ttgtaaaata agtccagagc ataacgaaat tcagatgttc gtaaaaatca tcaatacaga
      781 tttgagcaat gacacgctca aattcgactt tagtccagta gggaacgata gcttttcctt
      841 ttggaatagc tttagccttt ttagaaatat tattttccaa ataccccatt tctacagcat
      901 aatctaggga ttttctaaac ataccaaaga taagactagc ataggcttga gaataaccag
      961 agccgttttc ttccttcctt gatagtaacc atattctata attctgaata tcttcaatag
     1021 ttaaattacg taacgtccta ttttcaaagc gttctgcaat tttttcaagg tttttgattc
     1081 taacagcata ggtgctttct tctacatacg atttataata tggaatataa caatctaaaa
     1141 taaattgctt tacgttcata ttgtagttag cgtaggcgtg agccttatga tattctcgtt
     1201 ttattctagt aagctctaaa taggcttctc tggcagtaga aaaaggtttc ccattgctat
     1261 cttttctgct tttcttacga attcgcttac cgctgatttt gtcaattcct agctctgttt
     1321 catagaaata ttttcctttc gtgtctataa acacaccact ataacgtgtt tttgccattt
     1381 gtcagtcctc atccagttct acaccgataa ggttttctac atttttacgt ggcactaatc
     1441 cgagcctacg gttattgtag aatggtatgc ctttattata caccaaatct tgctttaccg
     1501 cctgaataat actaatcgca gtatgttttt tataacctag ttttattaag tcgtctttgc
     1561 taatcaatgg tttcatacgc tcacagttcc tttcttattc ttatttttgt gccagttgaa
     1621 tttgcggtaa tatctccatt ctcatctatt gataggttgt aaatagcaat atcttcttta
     1681 tattcaagat tatctaaatg acctaaggaa aaactagggg ttaatccctc aaaatagttt
     1741 ctttctagat tttcataact tactatcggt tttattaaac cacgagatga aaaatatttt
     1801 ggctgtttat atccacttgg aatagctagt aaatcttttg tgatatattt tgtaatgtag
     1861 ttagcaactt ttttagaaga atcgattttt gtaacggtac taaatcctaa ttgatacgaa
     1921 tcaacattat agacttgctt gccattcttt tttatcaatc gaccagtttt agtatttctt
     1981 gcttttgtta attttggact aaaaccgcct gttactccat gaaaatgaag ccgtccattc
     2041 ttgtgtaatt ctggtatgaa aatgtaacga aattttccat atttttcacg ctgagctttg
     2101 agccaagtac gtattttctt ttttactatt aaataatcat gagaattgat tttcgcatcg
     2161 tcaaaagtta atgtccaaaa taaatcgaaa tcattacata atagatagtg acggattcgt
     2221 cttttgattc gatgagattg ttgatataac tcatcatagc ttggcttttt gcaagaagga
     2281 gtagtatctt taataccatt ttctcctaat cgtctaatct tgggatttct ataatgaatt
     2341 atttcaaaag tattcgagct atattgaact atttttgtgt tatatacttc tcttgtcatt
     2401 tatcctccgc ttatcatgcc tgcatgtcaa gtagcaggct aatacacaca tctagcgagc
     2461 tctggcttgc ttgggcaagc cagctcacta gatagttaat tcattctatc tgctttcttg
     2521 gaatagtttg aaagtctctt attttatctt tgaaatctct tgaattcaaa tcaaaatatg
     2581 gagtctcaaa atattttatt ccgtttgttt tcggagttag aatatatcct tttccaactc
     2641 ctcctctgta atcaaccaaa aggtcttttt tctctttgcc catgatacta taaaaagttt
     2701 cctcggatat actagcgtcg cttgcaagta ttattctagt ttgacattgt tcggtgattg
     2761 ccctaggtaa atcagaggcg aggagttttt ggcttgctag aataagcaat cctcttccag
     2821 aagctcttga ttttaaggcg agtattttta ataatctaac catttccttt tgttgagctg
     2881 gagttccaga ttctaatgct gccagtacct catcaaatat aagataatgg gctggtagag
     2941 agaaatcttt atatgtagtt gaaaaatcaa ctgaagaaaa ggagaagtag cgtttatata
     3001 tatcttccat ttcaaatacc atttgttcta gaagtagaac aatctcttct gcattgcgtg
     3061 cgacaggaat acctagattt tcaaaagtag cacctaaaga ggtttgtttt gcatcaatta
     3121 aacttacaga gtgcccttgt ttgattaata agccagctag tgtggctaag taaagtgatt
     3181 ttccagctcc actaggggct attataatct gatgactagc ctcactagct atatcgaaga
     3241 aaatattgtc ataaagtttt attggattgt gatttacaca gtaatcatca tcagtaatct
     3301 caaacaattc acttacggaa tatcttattg gattttcgcc gtatttaagg acaacaatat
     3361 tactatctat agtggattcc aaaagattcc attcttcgtg ggtagcctgg ataagaaatt
     3421 cggtcaacct tctagatatt tcttttactt gctgaatact aggtatagtc ccagacggat
     3481 tgagcttaat tacaatcctg tcagcttctt tataaaattt ccattcaact gtttttatct
     3541 gggttttatt agaagggagt tgtattaaat ctagtcgttc tgttgcaagc tcaagaatgc
     3601 ttaaaaatct gtatttccag ataggagaat aaactgaaaa taagagtctc ataccaatat
     3661 gaccgataaa aagagcgaca agagcagaag atacaataat acctatcttg taaacaacgt
     3721 ttaatatagg caacattgat gaaagaaagg taataggaag agatagtagg agaaccttaa
     3781 atagtttctt gagaatagca gattgctttc ctttacgaac ttctatggtt tctgatatag
     3841 ataaataagt aggggaatcc tcacgaaaat catatctact aaaatttcgt tttattttat
     3901 ttagtatcat gatttaccgc ccaataactt tacttctgaa attatgagtt tgaattctct
     3961 gtaagcaccg ctattccgac catcagacac ccatttcaat gctaaaagtg cttcatctaa
     4021 gtcgatggtt ttagctataa tagcttcgtt attgagtttc tttaaagatg cttcatcgtt
     4081 ttgtacttca atcgtaaagg gttttatagt gcttaaatct cctcctactt cttgaatagc
     4141 ttttacaatt tctggctgga gagcagataa aactttttta cttatggttc catcaatgac
     4201 ttcatttttt tcattacgct taggaatatc tctgcattca ataacaaaag cagtagtagg
     4261 aatatgttcg acattagaag ttccaataag tagatttttt aagctaggtg agctgaaact
     4321 cacattttca atatttatag acatacctat acctcagttg tttttattgt gtgcacactt
     4381 gataaattta tcaagaagtt catgacctcc ttgacaacct tattttcgca tttttgataa
     4441 atagaggtgt ggggataaaa tttttatgac aataaaaaaa tccccacacg aagtgaggga
     4501 aaaaatgtta taataaaggt ttaatcattt ttgtttatca aattttcaag tgttatttta
     4561 tctattattg gtaataaaac atactttgct agcaactcta acttatcagg aagatacttg
     4621 aaagtaagaa aagtatcttt gtttttcgaa gatagaagaa cagaatcttt ttctataaat
     4681 gaaaatattg aatcttcctc taaaatttct attgtatcaa attgattgta tgtaaaatca
     4741 ataaatttat ctttcagttc atcataattg gaactaccaa aagaaaaatt tacttgaaaa
     4801 tactctttga tagaatttgt atctagtaaa gataaattca atagatgctt ggcatattcc
     4861 ggtatttctt gaatatcaag tatcattgcg ataaaaaaat cttctaaata atcatctata
     4921 ttgtcacccc aaagtaattc gttttcatca tgaaaataat gctcttgttt gactagaaaa
     4981 tttatcacac cgggagttat caaatattct gcacgtaatt tgtctagact agaataactt
     5041 aaaatatttc ctattagttt ctcatcattt ggataaaaag atatattaga ttgatagcca
     5101 taatcttcat acactttttg aattcgttct gaacagtttt taaaaattat atgacacgag
     5161 tttttaaatt ttgttattgg cataagaata ttattcctcc ccttcatctg gaatataagt
     5221 aattacatca gatacgttac agtctaaaat aaaacacaaa gtatctaatg tagctgtagt
     5281 tattggtttt ccttttttca ttctatgtag cgtattgctt gataagccgt ggtgaaaaat
     5341 taactcatat tcagtcttac cttggttaaa taaagtttcg taaaaaggtt tatatgaaat
     5401 catctgttat ctcatttccg tatattgata atttcattct acatccgtca atttattgac
     5461 tatactcaat aaattgacta taattaagta cggagaataa gataggagat attacaatga
     5521 aactcgaaga ttttcagaca tatccattca aaagtctgca agaatatatt tcagacaaag
     5581 acagagcagt aaaatttagt tttttactac agggagatga acccgaccgc tctgaattga
     5641 ccaaattaat atatagtgag atttttgaat ggtataacga gaatgaacat agtggagaca
     5701 cacttataac atatcgtaca gctattttta gaaagtatta tggaaaatca tatcgcaact
     5761 tatctagaga taagcaaaat gaaattctta atgtcataaa atcatatact cattgtgatg
     5821 aagattttcc tttcgaattt gaacttgagg ataaagaaaa tcccgtttat cagatttgta
     5881 ataattatca aataggaaat tttgggattt ttccaaaagg aagaattaat ccaaaaagag
     5941 ctcagtctcc atacaatgat tattttgacc ttgctttatc agtcatatgg gagttatatg
     6001 atggtacatt gactccggat gacgagttta aaaaagccat tttagatgaa aaagaatatt
     6061 ttaaccaatt tgaagacttt gaaacgtttt tatctgaaaa ttttttaaca gtattctttg
     6121 atgatgaggg atacttaatt cgtttatctg aaattgacag ttttgaagaa tatgtaagaa
     6181 tgagcaacca aatcatttat acaaggggaa ttgctatcct taac
//
LOCUS       DATRNK010000085         5938 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.085, whole genome shotgun sequence.
ACCESSION   DATRNK010000085 DATRNK010000000
VERSION     DATRNK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.085"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            434..1129
                     /locus_tag="VBC52_001856"
     CDS             434..1129
                     /locus_tag="VBC52_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO6883415.1"
                     /translation="MSKKSHRQYQIYEGLRCAVALCFISGYINAFTYVTQGKRFAGVQ
                     TGNLLSFAIHLSNKHYSQALAFLLPIMVFMLGQSFTYFMNRWANKHQLHWYLLSSFAL
                     TQVAIVTIILTPFLPSSFTVAGLAFFASIQVDTFKSLRGAPYANMMMTGNIKNAAYLL
                     TKGLYEKNSDIFLIARNTIIIIGGFIFGVVCSTYFSSKLGEWSLSLILIPLLYVNLLL
                     GHEFYNLQVERHS"
     gene            complement(1287..2162)
                     /gene="tehB"
                     /locus_tag="VBC52_001857"
     CDS             complement(1287..2162)
                     /gene="tehB"
                     /locus_tag="VBC52_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEO6883416.1"
                     /translation="MINTKTLLPYKTMPVWTAQSIPKAFLEKHNTKEGTWAKLTILSG
                     SLVFYQLSPDGEEISRHIFDASSDIPFVDPQVWHKVSPNSPDLSCYLTFYCQKEDYFH
                     KKYGLTRTHSEVIASAPLLSEKSNILDLGCGQGRNSLYLSLLGHQVTSVDSNGQSLVA
                     LENMALEEELPYNIKWYDINTATIEGHYDFILSTVVFMFLNPDCISDIILQMQSHTQI
                     GGYNLIVSAMDTTENPCPLPFPFTFKEGQLKSYYNDWEIIKYNENLGELHRVDENGNR
                     LKLQFATLLARKLRS"
     gene            2305..4302
                     /gene="metG"
                     /locus_tag="VBC52_001858"
     CDS             2305..4302
                     /gene="metG"
                     /locus_tag="VBC52_001858"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEO6883417.1"
                     /translation="MTEKKSFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRMMGFD
                     VQYLTGLDEHGQKIQQKAEEAGITPQEYVDGMAESVKTLWELLDISYDKFIRTTDTYH
                     EEAVAKIFEQLLAQGDIYLGEYTGWYSVSDEEFFTESQLAEVYRDENGNMIGGVAPSG
                     HEVEKVSEESYFFRMSKYADRLKAYYAEHPEFIQPDGRMNEMLKNFIEPGLEDLAVSR
                     TTYTWGVQVPSNPKHVIYVWIDALMNYISALGYGWSDDLSQYHKFWPADIHMIGKDIL
                     RFHSIYWPIMLMALDLPLPKRLVAHGWFVMQDGKMSKSKGNVVYPEMLVERFGLDPLR
                     YYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTIAMVNKYFDGEVPRFAVAT
                     DFDADLASVATDSIENYHKQMEAVDFPRALEAVWNLISRTNKYIDETAPWVLAKDETD
                     RDKLEAVMSHLAASLRVVAHLIQPFMMETSDAIMEQLGLGTTFDLEKLTFAGLPEGVR
                     VVAKGSPIFPRLDMEDEITYIKEQMNAGKAPVEKEWVPEEVELTSSKGQIKFEDFDAV
                     EIRVAEVIEVEKVEGSDKLLRFRLDAGDEGHRQILSGIAKFYPNEQELVGKKLQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTVLTVDSAVANGSIIG"
     gene            4498..5835
                     /gene="brnQ"
                     /locus_tag="VBC52_001859"
     CDS             4498..5835
                     /gene="brnQ"
                     /locus_tag="VBC52_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO6883418.1"
                     /translation="MVKKGFLTGLLLFGIFFGAGNLIFPPALGVASGQDFWPAILGFC
                     LPGVGLAIITLLLGTLTNGGYKTEMSEKFSPWFSLTFLVILYLTIGPLFAIPRTATVS
                     FEIGVAPIVGHGPIALLCFTACFFAAAYYLAIRPNGILDSVGKILTPVFAFLILSLVV
                     VGAIAYGNLESAKASADYAGKAFGSGVLAGYNTLDALAAVAFCLVATETLKKFGFKTK
                     KEYLSTIWIVGIVTSLAFSILYIGLGFLGNKFPVPADILADPNVNKGAYVLSQASYKL
                     FGNFGRYFLSIMVTLTCFTTTVGLIVSVSEFFDKNFRFGNYKLFATVFTLIGFLIANL
                     GLNAVITFSVPVLTLLYPIVIVIVLIILINKWLPLSKKGMSLTIGLVTLVSFVEVLAG
                     QWQEKTLTQLVGFLPFHTISMGWLVPMLIGLVFSLVLSDKQKGQAFDLEKFEG"
BASE COUNT         1824 a          922 c         1156 g         2036 t
ORIGIN      
        1 tcaatccaag aaaaaatgag agcaaaagga aagatatctt taaaactttt ataaattaga
       61 taacggcaat gatgacattt ataaatagtg ctgtgcataa tctgtactga cctcctaata
      121 ggttgtattt attgtaacaa atgttaagaa tggtgcaagt tttttggtag taaaattaaa
      181 agtaactaat taaacttcaa attaagggta aatgactcat gagattttct ttatcctttc
      241 ataaagtgag attgcttcaa tgctgaggct atgatatact aaaactacat ttatcattgc
      301 ttaataattt atttagaact gttaggctag ctagtgctaa tattaatact attttaaacc
      361 aatctattag aaaaaaagtg ctgagcaaga ttgcccagtc ttatttttgt ttgccataaa
      421 gttagaggaa aatatgtcta aaaagtctca ccgtcaatat caaatatatg aagggctcag
      481 gtgtgcggtt gcactctgtt ttatttcagg ttatatcaat gctttcactt atgtaacaca
      541 aggaaaacgt tttgcaggtg tacaaacagg gaatttgtta tcttttgcta ttcaccttag
      601 taataagcat tatagtcaag cgcttgcttt tttgctacct ataatggttt tcatgttagg
      661 tcaatcgttt acttatttca tgaatcgttg ggcgaataaa catcaactgc attggtacct
      721 attatctagt tttgcgttga cacaggttgc tatagtaaca attatattga ctcctttctt
      781 accatcgtcg tttactgtgg caggactagc cttctttgct tcaatacaag tagacacctt
      841 taaatcgctc agaggagcac cttatgctaa tatgatgatg actggtaata tcaagaatgc
      901 tgcttattta ttaacaaagg gactttacga aaaaaattct gatattttct taattgctag
      961 aaatacgatt attattattg gaggatttat ctttggtgtt gtttgctcga cttatttttc
     1021 aagtaaactg ggcgaatgga gcttatcatt aatattaatt cctcttttat atgtcaactt
     1081 gctgttaggc catgaatttt ataacctaca agtagaaagg cactcctaat gtatggcgtt
     1141 ttatgttgaa gagctatact aaggctgatt gttacaacaa tactttgaaa tatcatctat
     1201 atatatttta taggataatt atgtatgggt ttagaacata tttattacct aaccaaagat
     1261 aaaagtgagt agatttctac tcacttttat gatcttaatt ttcgagctaa taaagtcgca
     1321 aactgtaatt ttaagcgatt accgttttca tccaccctgt gcaattcacc taaattttca
     1381 ttatatttga tgatttccca gtcattgtaa taagatttca attgtccttc tttgaaggta
     1441 aaagggaatg gaagcgggca aggattttca gtggtatcca tagcagaaac gataagattg
     1501 tatcctccaa tctgagtatg tgattgcatt tgtaaaataa tatcagaaat gcagtcggga
     1561 tttaaaaaca taaataccac agttgataaa ataaaatcat agtgcccttc aatagtagca
     1621 gtattaatat cataccattt tatattgtaa ggaagctctt cttctaatgc catattttct
     1681 aaagctacaa ggctctgtcc gtttgaatcg acagaagtca cttgatgtcc cagcagcgat
     1741 aaataaagtg agtttcgccc ttgaccacac ccaaggtcta atatattact cttctcagat
     1801 aagagaggtg cactggcgat aacctcagaa tgtgtgcgcg tgagaccata ttttttatgg
     1861 aagtaatctt ctttttggca gtaaaaagtt agatagcaac ttaagtctgg actatttggc
     1921 gaaactttat gccagacttg tggatcaaca aaaggaatat cactactagc atcaaaaata
     1981 tgccgcgaga tttcctctcc atcaggagat aactggtaaa atactaaaga accacttaga
     2041 atggttagtt ttgcccaggt gccttcctta gtattatgct tttctaaaaa tgcctttgga
     2101 atagactgtg cagtccaaac cggcatagtt ttgtagggga gaagtgtttt tgtattaatc
     2161 atatattaac cctctatgtt taataccttt attatagcaa aagtagatat attattcaga
     2221 gatatatcca gttgaaaaag attttatgtt ataatgtatc atgataaaaa acacctctca
     2281 ttagggtggc ttataggaga aatcatgaca gaaaaaaaat cattttacat tactacccca
     2341 atttattacc catcaggtaa attgcacatc ggttctgcct atacaacgat agcatgtgat
     2401 gttttggcac gttacaaacg catgatgggc tttgatgttc agtatttaac tggtcttgat
     2461 gagcatggtc agaaaattca gcaaaaagct gaagaagcag gcattacacc acaggaatat
     2521 gttgatggca tggctgaaag tgtcaaaaca ttatgggaac ttctagatat ttcctatgac
     2581 aaattcatcc gtacaacaga tacttaccat gaagaagcgg ttgctaaaat ttttgaacaa
     2641 ttgttggcac aaggggacat ctatcttggt gagtatactg gatggtattc tgtttcagat
     2701 gaagaatttt ttactgaaag ccaattagca gaagtttatc gtgacgaaaa cggtaatatg
     2761 attggtggtg ttgcgccatc tggtcatgaa gttgaaaaag tttctgaaga atcttatttc
     2821 ttccgcatga gcaaatatgc agatcgttta aaagcatact acgctgagca tccagaattt
     2881 attcagccag atggtcgtat gaatgaaatg cttaaaaact ttatagaacc aggtttagaa
     2941 gatttagcag tgtctcgtac aacttacaca tggggagttc aggtaccatc aaatccaaaa
     3001 catgttattt atgtctggat tgatgctttg atgaactata tttctgctct tggttatggt
     3061 tggtctgacg acctcagtca atatcataaa ttttggccag cagacattca tatgattggt
     3121 aaagatattc ttcgtttcca ctctatttat tggcctatca tgttaatggc gctggatttg
     3181 ccattaccaa aacgtctagt tgctcatggt tggtttgtga tgcaagatgg taaaatgtct
     3241 aaatcaaaag ggaatgtcgt ttatccagaa atgttagtag agcgttttgg tcttgatcca
     3301 ctccgttatt atctcatgcg ttcattgcct gttggttcag acggaacttt tacgccagaa
     3361 gattatgttg gacgtattaa ctatgaactt gctaatgatt taggaaacct tctcaaccgc
     3421 actattgcta tggttaacaa gtatttcgat ggagaagtac caagatttgc tgttgcaact
     3481 gactttgatg ctgatttagc ttctgttgcg acagatagta ttgaaaacta tcataaacaa
     3541 atggaagcgg tggatttccc acgtgccctt gaagctgtat ggaatcttat ttcacgaact
     3601 aacaaatata ttgatgaaac agcaccgtgg gtacttgcaa aagacgaaac cgatcgtgat
     3661 aagctagaag ctgtaatgag tcatttagct gctagtctac gtgttgttgc tcacctcatt
     3721 caaccgttca tgatggaaac ttccgatgcc attatggaac aacttggttt agggacaaca
     3781 ttcgatcttg aaaaattgac ttttgctggt ctaccagaag gtgttcgagt tgttgcaaaa
     3841 ggttcaccaa ttttcccaag gttggatatg gaagacgaaa ttacttatat caaagaacaa
     3901 atgaatgctg gaaaagcacc agtggaaaag gagtgggttc ctgaagaagt tgaattgaca
     3961 tcaagcaaag gtcaaatcaa gtttgaggat tttgacgctg ttgaaatccg cgttgcagaa
     4021 gtaattgaag ttgagaaagt ggaaggttct gataaacttc ttcgtttccg tctagatgct
     4081 ggtgacgagg gccatcgtca aatcttatca gggattgcaa aattttaccc aaatgaacaa
     4141 gagttagtag ggaaaaaact ccaaattgtt gctaacctca aaccacgtaa aatgatgaaa
     4201 aaatatgtca gtcaaggcat gattctctca gcagaacatg atggtaaatt gacagtgttg
     4261 acggtcgatt cagcagtggc aaatggatca attattggtt aatcaaaaag agcattatgc
     4321 tctttttttg ttttcaagaa aaaagtgact gaatagttgg aaaattatga aaatgatcta
     4381 gaatttattt ttatttttga tataataaaa gacatcgatt ctaagcattt agatagaatt
     4441 tgataaggag aatttatgga aagggtgtaa atagattatt tacactaatg gttgcttatg
     4501 gtaaaaaaag gctttttaac gggattactt ttatttggta tcttctttgg tgcagggaac
     4561 ctcatttttc caccagcatt aggtgtagca tctggacaag atttctggcc agcgatttta
     4621 ggattttgtt tgccaggagt tggattagct attattaccc tcctattagg cacactaact
     4681 aatggtggtt ataagacaga gatgtcggaa aaatttagtc cgtggttttc tttaacattt
     4741 ttagttatac tctacctaac tattggtcca ctttttgcta ttcctagaac agcaacggtt
     4801 tcttttgaaa ttggggttgc tccaattgta gggcatggtc cgattgctct tttgtgtttt
     4861 acagcctgtt tttttgctgc agcttattat ctcgctatta gaccaaatgg tatattagat
     4921 agtgttggga aaatcttaac acctgttttt gcctttttaa ttctctcttt ggttgtcgtt
     4981 ggagctattg cttatggaaa cttggagagt gctaaggcga gcgctgacta tgcaggaaaa
     5041 gcgtttggaa gtggtgtctt agcaggctat aatacacttg atgcacttgc agcagttgct
     5101 ttttgtctag tagcaacaga aacgcttaaa aagtttggtt ttaaaactaa aaaagaatat
     5161 ttatcaacca tttggattgt tggaattgtt actagtttag catttagtat tttatatatt
     5221 ggcttaggtt ttttagggaa taagtttcct gtcccagctg atattttagc tgacccaaat
     5281 gttaataaag gagcttatgt attatcacaa gcatcttata aattatttgg aaattttggt
     5341 cgttatttct tgagtattat ggtaacattg acatgcttta caacaacagt tgggcttatt
     5401 gtgtcagtat ctgaattttt tgataagaac tttcgttttg ggaattataa attatttgct
     5461 actgttttta ctttgattgg ctttttaatt gctaatcttg gtttaaatgc tgtaattact
     5521 ttttcagttc ctgtgctaac tttattgtat ccgattgtta ttgttattgt cttaattatt
     5581 ttgattaata agtggttgcc gctttcaaaa aagggaatgt ctttgacaat tggtcttgtc
     5641 actttagtgt cgtttgttga ggttctagcg ggacaatggc aagaaaaaac attaactcag
     5701 ttagtaggat tcctcccctt tcatactatt tcaatggggt ggctagttcc aatgttgatt
     5761 ggtctagtat tttctttggt gctgtcagat aaacaaaaag gccaagcatt tgatttagag
     5821 aaatttgaag gatagcacta tttcaatttt atagatatgt caaaaaggtt aaaacttagt
     5881 tttagccttt ttttagatca gattttttaa aatgatttat agattgcagt aaggtgtg
//
LOCUS       DATRNK010000086         5916 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.086, whole genome shotgun sequence.
ACCESSION   DATRNK010000086 DATRNK010000000
VERSION     DATRNK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.086"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            100..1755
                     /locus_tag="VBC52_001860"
     CDS             100..1755
                     /locus_tag="VBC52_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6883419.1"
                     /translation="MAKQKNNWRRVGVGVLTLASVATLAACGSKSASQDSNGAINWAI
                     PTEINTLDLSKVTDTYSNLAIGNSSSNFLRLDKDGKTRPDLATKVDVSKDGLTYTATL
                     RKGLKWSDGSKLTAKDFVYSWQRLVDPKTASQYAYLAVEGHVLNADKINEGQEKDLNK
                     LGVKAEGDDKVVITLSSPSPQFIYYLAFTNFMPQKQEVVEKYGKDYATTSKNTVYSGP
                     YTVEGWNGSNGTFTLKKNKNYWDAKNVKTKEVRIQTVKKPDTAVQMYKRGELDAANIS
                     NTSAIYQANKNNKDVTDVLEATTAYMEYNTTGSVKGLDNVKIRRALNLATNRKGVVQA
                     AVDTGSKPAIAFAPTGLAKTPDGTDLAKYVAPGYEYNKTEAAKLFKEGLAESGLTKLK
                     LTITADADAPAAKNSVDYIKSTWEAALPGLTVEEKFVTFKQRLEDSRKQNFDIVVSLW
                     GGDYPEGSTFYGLFKSDSQNNDGKFANKDYDAAYNKAISEDAMKPAESAKDYKEAEKI
                     LFEQGAYNPLYFRSGKGLQNPKLKGVIRNTTGLSIDFTHAYKK"
     gene            1874..2788
                     /locus_tag="VBC52_001861"
     CDS             1874..2788
                     /locus_tag="VBC52_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6883420.1"
                     /translation="MIKYILKRVAILLVTLWVVITLSFFLMQILPGTPYNNPKLTEEM
                     IALLNKQYGLDKPVWQQYLTYLWNVLHGDFGTSYQSVNQPVSRMISLRLGVSVHLGVQ
                     ALVFGVLGGILVGAISARHKNDKVDGILSVIATLGISMPSFIIGILLLDYFGFKWNLL
                     PLSGWGTFSQTILPSLALGLPTLASVSRFFRSEMIETLNSDYVQLARSKGMTIRQVTR
                     KHAYRNSMIPILTLIGPLAAGLLTGSALIEQIFSIPGIGQQFVTSIPTKDYPVIMGTT
                     IVYAVMLMVAILITDVVISIVDPRVRLQ"
     gene            2798..3829
                     /locus_tag="VBC52_001862"
     CDS             2798..3829
                     /locus_tag="VBC52_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6883421.1"
                     /translation="MADKNRTFKLVGAGSSSTQEKIEKPALSFMQDAWRRLKKNKLAV
                     VSLYLLALLLTFSLASNLFVTQKDANGFDSKKVTTYRNLPPKLSSNLPFWNGSIKYAG
                     NTESTDAYKSQNVPEKVKYALGTDSLGRSVAKRIIVGIRISLLVAIAATFIDLIIGVT
                     YGLVSGFAGGRLDTLMQRIVEVISSIPNLVIVTMLGLVLGNGITAIIISIAFTGWTSM
                     SRQVRNLTLSYREREFVLAARSLGESPIKIAFKHILPNISGIIIVQIMMTIPSAIMYE
                     AVLSAINLGVKPPTASLGSLISDAQENLQYYPYQVILPALALVMISLAFILLGDGLRD
                     AFDPKSSDE"
     gene            3842..4888
                     /locus_tag="VBC52_001863"
     CDS             3842..4888
                     /locus_tag="VBC52_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6883422.1"
                     /translation="MEKETILSVNNLHVDFHTYAGEVKAIRDVNFELKKGETLAIVGE
                     SGSGKSVTTRTLIGLNAKNSEISGNVQFKGRNLVELSEEEWTKVRGNEISMIFQDPMT
                     SLDPTMKIGMQIAEPMMIHQKISKKDALKLALELMKDVGIPNAEEHINDYPHQWSGGM
                     RQRAVIAIALAADPEILIADEPTTALDVTIQAQILNLMKKIQAERDSSIVFITHDLGV
                     VAGMADRVAVMYAGKIVEFGTVDEVFYNPQHPYTWGLLNSMPTTDTESGSLESIPGTP
                     PDLLNPPKGDAFAARNEFALDIDHEEEPPYFKVSETHFAATWLLDERSPKVLPPLPIQ
                     KRWEKWNEIEGRKA"
     gene            4888..5820
                     /locus_tag="VBC52_001864"
     CDS             4888..5820
                     /locus_tag="VBC52_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6883423.1"
                     /translation="MTENRKKLVEVKNVSLTFNKGKANEVRAIDNVSFDIYEGEVFGL
                     VGESGSGKTTVGRSILKLYDISDGEITFNGEVISHLKGKALHSFRKDAQMIFQDPQAS
                     LNGRMKIRDIVAEGLDIHKLAKSKSDRDSKVQALLDLVGLNKDHLTRYPHEFSGGQRQ
                     RIGIARALAVEPKFIIADEPISALDVSIQAQVVNLMQKLQREQGLTYLFIAHDLSMVK
                     YISDRIGVMHWGKLLEVGTSDDVYNNPIHPYTKSLLSAIPEPDPESERQRVHQPYNPA
                     IEQDGQERQMHEITPGHFVLSTPQEAEEYKKQIL"
BASE COUNT         1919 a         1000 c         1170 g         1827 t
ORIGIN      
        1 ttaaaaaatt atctgaaaat tttgcgtttt tgtcttttct taagaatttt ttgtggtata
       61 atgttagtca ctagataaat gaattaggag aacaaagcta tggcaaaaca gaaaaataac
      121 tggcgccgtg ttggagttgg tgtccttaca cttgcttcag ttgcgactct tgctgcatgt
      181 ggaagtaaat cagcttccca ggattctaat ggagcgatta attgggctat tccaacagaa
      241 atcaatacac tagatttatc taaagtcaca gacacttact caaatctagc tattggtaac
      301 tctagtagta atttccttcg cttagataaa gatggaaaga caagaccaga tttggctact
      361 aaagtagatg tttcaaaaga tggcttaact tatacagcta cattacgtaa aggcttgaag
      421 tggtcagatg gcagtaaact tactgcaaag gattttgttt attcatggca acgtttagtt
      481 gatcctaaaa cagcttcaca atatgcttac cttgctgttg aagggcatgt gcttaatgcc
      541 gataaaatca acgaaggaca agagaaagac ttgaataagc taggtgttaa ggcggaaggc
      601 gatgacaaag ttgttattac tttatctagt ccgtctccac aatttatcta ctaccttgca
      661 ttcactaact tcatgccaca aaaacaagaa gttgttgaaa aatatggaaa agattacgca
      721 actacttcaa aaaatacagt ttactcagga ccatatactg ttgaaggttg gaatggttcg
      781 aatggtactt tcacgctgaa gaaaaacaaa aattattggg acgctaaaaa tgtaaaaaca
      841 aaagaagttc gcatccagac tgttaaaaaa ccagataccg ccgttcaaat gtataaacgt
      901 ggtgagttag atgcagctaa tatctcaaat acttctgcta tttatcaagc taataagaat
      961 aataaagatg tcacagatgt tctagaagcg accactgcct atatggaata taatactact
     1021 ggttctgtga aagggcttga taatgttaag attcgtcgcg ccttaaactt agcaactaac
     1081 cgtaaaggag ttgttcaagc agccgttgat acaggctcaa aaccggcaat tgcttttgca
     1141 cctactggtt tagccaaaac accagatgga actgatttgg caaaatatgt tgccccaggt
     1201 tatgaatata ataaaactga agcagcaaaa ctctttaaag aaggtttggc tgaatcaggc
     1261 ttgactaagc taaaattaac aattacagca gatgctgatg ctcctgctgc caaaaactct
     1321 gttgactata tcaagtctac ttgggaagct gctcttccag gacttactgt tgaagaaaaa
     1381 tttgtaacct ttaaacaacg cttagaagac agtagaaaac aaaactttga catcgtagtt
     1441 tctctttggg gtggggatta tccagaagga tcaactttct acggcctctt taagtcagat
     1501 tcacaaaata acgatggaaa atttgctaac aaggactatg atgctgctta taacaaggca
     1561 atttctgaag atgcgatgaa accagcagaa tcagcgaagg actataaaga agcagagaaa
     1621 attttatttg agcaaggtgc ttataaccca ctctacttcc gtagtggtaa aggtttacaa
     1681 aatccaaaat taaaaggtgt tattcgtaat actacaggtt tgtcaataga ctttacacat
     1741 gcttataaaa aataattaaa ttactttaca ctaaaccttt gcaagaattg gtaattgcca
     1801 gttcttgctt ttggttaagt aaagaaagtg aaagagtttg ctatttttag caattctaag
     1861 aaaaggaaat gatatgatta aatatatttt aaagcgtgtt gctattttgt tagtaacgct
     1921 atgggttgtc ataaccctat cgttctttct gatgcagatc cttccaggaa caccctacaa
     1981 taatccaaaa ctcactgaag aaatgatcgc attgcttaat aaacagtatg gtttagacaa
     2041 gcctgtatgg caacaatatt tgacttacct ttggaatgta cttcatggtg actttggaac
     2101 gagttatcaa tcagtgaatc agccagtatc acgtatgatt agcttgagat taggagtttc
     2161 agtacatcta ggtgttcagg ctctcgtatt tggtgtttta ggcggtattt tggtaggagc
     2221 tatttcggca cgtcataaga atgataaagt cgatggtatc ttaagtgtta ttgcaacgct
     2281 agggatttct atgccttcat ttatcattgg tatcttatta ttagactatt ttggatttaa
     2341 gtggaacttg ttgcctttat caggttgggg gactttctct caaaccattt taccttcttt
     2401 agctctagga ctccctacat tagcatctgt ttcacgcttc ttccgtagtg aaatgattga
     2461 aactttaaat tctgattatg tgcaactagc tcgttcaaaa ggtatgacaa ttcgtcaagt
     2521 aactcgaaaa catgcttacc gtaattctat gattccaatt ttaactctaa ttggcccttt
     2581 ggcggcgggt ctgttaactg gttctgcact aattgagcag attttctcga ttccaggaat
     2641 tgggcagcag tttgttactt cgattccaac aaaagactat cctgttatta tggggacaac
     2701 cattgtttat gcagtaatgt tgatggttgc tatcttgata acagacgttg ttattagtat
     2761 cgtagaccca cgtgttcgct tgcagtaagg agatgttatg gcagataaaa acagaacatt
     2821 taaacttgta ggtgcaggat cttctagcac acaagaaaaa attgaaaagc ctgctctttc
     2881 gtttatgcaa gatgcgtggc gtcgcttgaa aaaaaacaaa ttagcagtag tttcactcta
     2941 tttattagct cttttactta ctttttcgtt agcctcaaat ttatttgtaa ctcagaagga
     3001 tgctaatggg tttgattcga aaaaagtaac tacatatcgc aacttaccac ctaaattgag
     3061 ttcaaacctt cctttttgga atggtagcat taagtacgct ggaaatacag aatctacaga
     3121 cgcttataag tctcaaaatg tccctgaaaa agttaagtat gctttaggta cagattctct
     3181 agggagaagt gttgctaagc gcatcatcgt aggtattcgg atttccttac ttgtagctat
     3241 tgcagctaca ttcattgatt taattatcgg tgttacatac ggtcttgttt caggttttgc
     3301 tggaggacgc cttgatacat taatgcaacg tatcgtagag gtaatttcat caattccaaa
     3361 cttagttatc gttacaatgt taggtttggt ccttggtaac ggtattacgg ctattattat
     3421 ctctattgct tttaccggat ggacttctat gtctcgtcag gtaaggaatt taaccctatc
     3481 atatcgagaa agagagtttg tgcttgctgc acgttcactt ggggaaagtc caattaaaat
     3541 agccttcaaa catattttac ctaacatttc tggtattatc attgtacaaa ttatgatgac
     3601 aatcccaagt gctattatgt atgaagcggt actttcagca atcaatttag gtgttaaacc
     3661 accgacagct tcattaggat cattgatttc agatgcacaa gaaaatttac aatattatcc
     3721 atatcaagtt atcttgccag ctttagcatt ggtaatgatt tcattggcat ttattttgct
     3781 tggtgatggg ttgcgagatg cttttgatcc aaaatctagc gatgaataag gaggtataat
     3841 catggaaaaa gaaactattt taagtgttaa taatcttcat gttgacttcc acacatatgc
     3901 tggagaagta aaagcaattc gtgatgtcaa ctttgaatta aaaaaaggtg agactcttgc
     3961 aatcgttggt gaatctggtt caggaaaatc tgtaactacc agaactttaa ttggtttaaa
     4021 tgctaaaaat tcagagatat cagggaatgt tcaatttaag gggcgtaacc ttgttgaact
     4081 atcagaagaa gagtggacta aggtacgcgg gaatgagatt tctatgattt tccaagaccc
     4141 tatgactagt ttggatccaa cgatgaaaat tggcatgcaa atagcagaac caatgatgat
     4201 tcatcaaaaa atttcaaaaa aggatgcttt aaaattagct cttgagctaa tgaaagatgt
     4261 aggtatccca aatgctgaag agcatattaa tgattaccca catcagtggt ctggaggaat
     4321 gcgccaacgt gcggttatag cgattgcttt agctgctgat cctgaaattc ttattgctga
     4381 tgaaccaacg actgctttag atgtaacaat tcaagcacaa attttgaact taatgaaaaa
     4441 aattcaagca gaacgagact cctccattgt tttcataact catgacttag gggtcgttgc
     4501 agggatggca gaccgtgtag cagttatgta tgcagggaaa attgttgaat ttggaactgt
     4561 tgatgaagtc ttttataatc cacaacatcc atatacttgg ggattattga attcaatgcc
     4621 gacaaccgac acagaatcag gtagtttaga gtcaattccg ggaacaccac cagatttgtt
     4681 gaacccacct aaaggagatg catttgcagc tcgtaatgaa tttgcattag atattgatca
     4741 tgaagaagaa ccaccgtact tcaaagtttc agaaacacat ttcgctgcta catggctttt
     4801 agatgaaaga tcacctaagg tgcttcctcc attaccgatc caaaaacgtt gggaaaaatg
     4861 gaacgagatt gaagggagga aagcctaatg actgaaaatc gaaaaaaatt agttgaagtc
     4921 aaaaatgttt ctttgacctt caataaagga aaagctaatg aagtgagagc aattgataat
     4981 gttagttttg acatttatga aggtgaagtg tttggattag ttggggaatc tggatcagga
     5041 aagacaaccg ttggacgttc aattttaaaa ctttatgata tttctgatgg agaaattacc
     5101 tttaatggag aagtgatctc acatctgaaa ggtaaagcat tacatagttt tcgtaaagac
     5161 gctcagatga tttttcagga tcctcaggct agcttaaacg gccgtatgaa gattcgtgat
     5221 atcgtagcag aaggtttaga tatacataaa ttagctaaat caaaatcaga tcgtgatagt
     5281 aaagttcagg ccctattaga tcttgtaggg ttaaataaag atcatttaac acgttatccg
     5341 catgaatttt caggtggaca acgacaacgt atcgggattg cacgtgcttt agcagtagag
     5401 cctaaattta tcattgctga tgaaccaata tctgccttag atgtttcaat ccaagcacaa
     5461 gttgttaatt taatgcaaaa attgcaaaga gaacaaggat tgacttattt gtttatcgca
     5521 catgatttgt ctatggtaaa atacatttca gatcgtatcg gtgttatgca ttgggggaaa
     5581 ctgttagaag ttggaacatc tgatgatgtt tataacaatc caatccaccc ttatacgaag
     5641 agtctattat cagctattcc agaaccagat ccggagagtg aacgtcaacg tgttcaccag
     5701 ccgtataatc cagctattga gcaagacgga caagaacgtc aaatgcacga gattacccct
     5761 ggtcattttg ttttatctac accccaagaa gctgaagaat ataaaaaaca aatattataa
     5821 aagatggcta gcccatcttt tattttttta ctaataaaat aaatttaggt atggaatttt
     5881 aaactctatg ttattggtgt tagccaacag gaaact
//
LOCUS       DATRNK010000087         5676 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.087, whole genome shotgun sequence.
ACCESSION   DATRNK010000087 DATRNK010000000
VERSION     DATRNK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.087"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            113..1258
                     /locus_tag="VBC52_001865"
     CDS             113..1258
                     /locus_tag="VBC52_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529591.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO6883424.1"
                     /translation="MGQEPIIEYQNINKVYGENVAVEDINLKIYPGDFVCFIGTSGSG
                     KTTLMRMVNHMLKPTNGTLLFKGKDISTINPIELRRRIGYVIQNIGLMPHMTIYENIV
                     LVPKLLKWSEEAKRAKARELIKLVELPEEYLDRYPSELSGGQQQRIGVIRALAADQDI
                     ILMDEPFGALDPITREGIQDLVKSLQEEMGKTIILVTHDMDEALKLATKIIVMDNGKM
                     VQEGTPNDLLHHPATSFVEQMIGEERLLHAQADITPVKQIMLNNPVSITAEKTLTEAI
                     TLMRQKRVDSLLVTDNGKLIGFIDLESLSSKYKKDRLVSDILKHTDFYVMEDDLLRNT
                     AERILKLGLKYAPVVDHENNLKGIVTRASLVDMLYDIIWGDTETEDQ"
     gene            1258..1893
                     /locus_tag="VBC52_001866"
     CDS             1258..1893
                     /locus_tag="VBC52_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6883425.1"
                     /translation="MVNFLSQYGMQILVKTWEQVYISFFAIALGIAIAVPLGVVLTRF
                     PKVAKIIIAIASMLQTIPSLALLALMIPLFGIGKIPAIVALFIYSLLPILRNTYIGMN
                     NVNPTLKDCAKGMGMKPIQSIFQVELPLATPIIMAGIRLSTIYVISWATLASYIGAGG
                     LGDLIFSGLNLFQSKLILGGTIPVIILSLIIDYLLGLLETALTPRTTRREA"
     gene            1896..2822
                     /locus_tag="VBC52_001867"
     CDS             1896..2822
                     /locus_tag="VBC52_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6883426.1"
                     /translation="MLKKSHFLQIFTLCLALLTISGCQLTDTKKSGHTTIKVAAQSST
                     ESSIMANIITELIHHELGYNTTLISNLGSSTVTHQALLRGDADIAATRYTGTDITGTL
                     GLKAVKDPKEASKIVKTEFQKRYNQTWYPTYGFSDTYAFMVTKEFARQNKITKISDLK
                     KLSTTMKAGVDSSWMNREGDGYTDFAKTYGFEFSHIYPMQIGLVYDAVESNKMQSVLG
                     YSTDGRISSYDLEILRDDKKFFPPYEASMVVNNSIIKKDPKLKKLLHRLDGKINLKTM
                     QNLNYMVDDKLLEPSVVAKQFLEKNHYFRGDK"
     gene            2823..3473
                     /locus_tag="VBC52_001868"
     CDS             2823..3473
                     /locus_tag="VBC52_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6883427.1"
                     /translation="MKQMNTFQQFIYYFQHNGSYILEQFIHHFLISVYGVILAAIVGI
                     PLGIFIARRGKIKTFVMGIANVIQTIPSLAMISIIMLGLGLGIKTVVATVFLYSLLPI
                     ITNTYTGIRNVDSDLLDAAKGMGMTKRQRLFMVELPLSISVIMAGLRNALVVAIGITA
                     IGAFVGGGGLGDIIIRGTNATNGGAIILAGSLPTALMAIFSDLILGGIQRMLEPRK"
     gene            complement(3535..4716)
                     /locus_tag="VBC52_001869"
     CDS             complement(3535..4716)
                     /locus_tag="VBC52_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6883428.1"
                     /translation="MNINGIKLLSSRAVSKLGDVFYDYGNSTWIASMGGLGQKILGIY
                     QIVELLVSIVLNPFGGALADRFQRRKILLITDAICAIMCFLLSFIGDDKVMVYGLIVA
                     NAILAVSNAFSSPAYKSYIPEIVDKADIITYNANLETIVQIISVSSPVLGFLIFNNFG
                     IRITLIVDAITFLISFLFLYAIKVERVQLSKQEKVAIKNILADIADGFTYIKKEKEIM
                     FFLIIAALLNTFLAMFNYLLPFTNSLLKTSGAYATILSISAIGSIIGALIARKIKSSI
                     NSMLSMLVFSSLGVIVMGFPSLFELPIWIPYSGSFLFNSLLTMFNIHFFSQVQIRVDE
                     AYMGRVMSTIFTIAIMFMPIGTLFMTIFSFALSNVSFIVIGCAIAILGGLGFSYSKKQ
                     F"
     gene            complement(4789..5649)
                     /locus_tag="VBC52_001870"
     CDS             complement(4789..5649)
                     /locus_tag="VBC52_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutR family transcriptional regulator"
                     /protein_id="HEO6883429.1"
                     /translation="MFKYVPIFREFRLNRQFSLKQVASNELSVSQLSRFERGESDLSL
                     TKFLGALEAIDLSISEFMDRVNKYQKSDQISLMSQMAQYHYQRDVAGLEKMISVEEGK
                     LKKDSSDIRCRLNIVLFRGMICECDSSRKMSEEDLCFLSDYLFQKDSWEISDYILIGN
                     LYRYYNTRHICQLVKEVINQKEYYRDIYTNRNVVEATLLNVVETLIERRALEEATFFL
                     EKVEALLNNERNAYHRIILLYEKGFLAYAKGDSRGIQSMKQAIFCFQAIGSKHHVENF
                     QEHFNRVTRL"
BASE COUNT         1918 a         1012 c          924 g         1822 t
ORIGIN      
        1 aggctcaagc ttaacgattc tttttaaaat cattaaattt taaaacaaat ttcagacatg
       61 ttgccaaagt tttgatatta ttactataat atagtttgta gaggagaata atatgggcca
      121 agaacctatc atcgaatatc aaaatatcaa taaagtgtat ggggaaaatg ttgcggttga
      181 agacattaac cttaaaattt accctggtga ttttgtttgt ttcatcggta cgagtggatc
      241 aggtaaaaca acattaatgc gtatggttaa ccatatgtta aaaccaacaa atggtactct
      301 attatttaag ggaaaagata tttctactat taaccccatt gaattaagac gcagaattgg
      361 atatgttatc caaaacattg gtttaatgcc tcatatgacc atttacgaaa atatagttct
      421 tgtaccaaaa ttattgaaat ggtcagaaga agctaaaaga gctaaagcaa gggaacttat
      481 taaattagtt gaattacccg aagaatattt ggatcgctac cctagtgagt tgtctggcgg
      541 tcagcaacaa cgtatcggtg tcattcgcgc tcttgcagca gaccaagata ttattttaat
      601 ggatgagcct tttggagctc tggatcctat tactagagaa ggtattcaag acttagtcaa
      661 gtctcttcag gaagaaatgg ggaaaactat catcttagtt actcatgata tggatgaagc
      721 cctcaagtta gcaacaaaaa ttattgttat ggacaatggt aaaatggtcc aagaagggac
      781 acccaatgat ctcttacatc atcctgctac tagtttcgtt gaacaaatga ttggagaaga
      841 gcgtctcctt catgcgcagg ctgatattac tcctgttaaa cagataatgt taaataatcc
      901 tgtttcaata actgctgaaa aaacactaac tgaagctatt acactaatgc gccaaaaacg
      961 cgttgactca cttctagtaa ccgataacgg taaattaatt ggttttattg acttagaatc
     1021 tctgagcagt aaatataaga aagatcgact tgtttctgat atcttaaaac atactgattt
     1081 ttatgttatg gaagacgact tacttagaaa tactgctgag cgtattttaa aacttggttt
     1141 aaaatacgct ccagttgttg accatgagaa taacttaaag ggcattgtta ctcgtgcatc
     1201 cctagttgat atgttatacg atattatttg gggcgatact gaaacggagg atcaataatg
     1261 gtcaatttct tatcacagta tggcatgcaa atattagtaa aaacatggga acaagtctat
     1321 atctcattct ttgctatagc acttgggatt gcaattgctg ttccattggg tgtggtctta
     1381 acacgttttc ccaaagtagc caaaattatc attgctatag ctagcatgtt acaaactata
     1441 ccaagtttag ccttattagc tttaatgatt ccattgtttg gaattggaaa aatcccagct
     1501 atcgttgctt tatttattta ttccttgctt cctatactaa ggaatactta tataggtatg
     1561 aataatgtca atccaacact taaagattgt gctaaaggga tgggaatgaa acctattcaa
     1621 tctatatttc aagttgagtt acctttagct actccaatta ttatggctgg tattcgcttg
     1681 tctactattt atgttatttc ttgggctaca ttggcttctt atataggtgc aggtggactt
     1741 ggtgatctca tttttagcgg gttaaatctt tttcagtcca aattaattct agggggcaca
     1801 attcctgtta ttatcctgtc acttattata gattatcttt taggattact ggaaacagct
     1861 ctaacgccca gaacaacaag aagggaggct tagatatgct taaaaaatcg cactttttac
     1921 agatatttac actttgctta gccctcttaa cgatttctgg ttgtcaatta accgatacta
     1981 aaaagtctgg tcataccaca attaaggttg ctgcccaaag ttctacagag tctagtatta
     2041 tggcaaatat tatcaccgaa ttaattcatc acgaattagg atacaacaca actttaataa
     2101 gcaatcttgg ttcctctacg gttactcacc aagctttgct ccgtggtgat gctgacattg
     2161 ctgccacacg ttatacagga acagacatca cagggactct tggtttaaaa gctgttaaag
     2221 accctaaaga agcttctaag attgtaaaaa ctgaattcca aaaacgctac aatcaaactt
     2281 ggtatcctac ttatggtttt tctgatactt atgcattcat ggttactaaa gagtttgcca
     2341 gacagaataa aatcactaag atctctgatc ttaaaaaatt atcaacaact atgaaggcag
     2401 gggttgatag ttcatggatg aatcgcgagg gagatggata cactgatttc gctaaaacat
     2461 acggttttga attttcacat atttacccta tgcaaattgg cttagtctat gatgcggttg
     2521 aaagtaacaa aatgcaatct gtattaggct actccactga cggtcgtatt tcgagctatg
     2581 atttagaaat tttaagggat gataaaaaat tctttcctcc ttatgaagcc tctatggttg
     2641 tcaacaattc tatcatcaaa aaagatccta aactaaaaaa attactccat cgactcgatg
     2701 gtaaaatcaa tttaaaaacg atgcaaaacc ttaattatat ggtagatgat aaacttttag
     2761 aaccttcagt tgttgccaaa caatttttag aaaaaaacca ttattttaga ggagataaat
     2821 aaatgaaaca aatgaatact ttccaacaat ttatctacta cttccaacat aatggtagtt
     2881 atatcttaga acaatttatc caccactttt taatttcagt ttacggcgtt attttagctg
     2941 caattgttgg tatccctcta ggtatcttca tcgctagacg tggtaaaatt aaaacctttg
     3001 ttatgggaat cgcaaatgtc atccaaacca ttccttcttt agctatgatc tctatcatta
     3061 tgttaggtct agggctaggt attaaaacag tagttgcaac tgtctttctt tattctcttc
     3121 tgccaatcat cacaaatact tacactggta ttagaaatgt tgactccgac ttactagatg
     3181 ctgctaaagg aatgggcatg actaagcgtc aacgtttatt tatggttgag ttaccactat
     3241 ctatttccgt tatcatggct ggacttagaa atgcccttgt tgtagcaatt ggtattacag
     3301 ccattggtgc tttcgtcggg ggaggcggac ttggagatat catcataaga ggaactaatg
     3361 caacaaatgg tggtgctatt atcttagcag gatctcttcc aacagccctt atggcaattt
     3421 tctctgattt aatcctaggc ggtatccaac gcatgcttga accaagaaaa taactattaa
     3481 aaacttgtca gtagagacaa gtttttttga tgtttcagaa taatttctta agttctaaaa
     3541 ttgtttttta ctatagctaa atcctaatcc tcccaatata gcaatggcac atcctataac
     3601 aataaagcta acatttgata aggcaaatga aaagatagtc ataaatagtg ttcctatagg
     3661 cataaacata atagcaatag taaatatagt cgacatcaca cgccccatat acgcttcatc
     3721 gaccctgatt tgtacctgac tgaaaaaatg aatattaaac attgtcagaa gactattaaa
     3781 aagaaaacta ccactgtaag gtatccatat cggtaattca aatagagatg ggaatcccat
     3841 aactatgact cccaaactac tgaatactag catagatagc atagaattaa tactagattt
     3901 aatttttcta gcaataagcg ctccaataat tgatccgata gctgatatac taagtattgt
     3961 tgcataagca cctgaagttt ttaataggga gttagtaaaa ggtaaaagat aattgaacat
     4021 tgctaggaaa gtgtttaata aagcagcaat aattagaaaa aacataattt ctttttcttt
     4081 tttgatgtaa gtaaatccat ctgcaatatc agctaaaata ttttttatcg ctaccttttc
     4141 ttgttttgaa agctgtaccc tttccacttt aatagcataa agaaacagga aagagatcaa
     4201 aaaagtaatc gcatcaacaa ttaaagtaat cctaatacca aaattattaa aaataaggaa
     4261 acctaaaaca ggggaactta cactaataat ttgcacaatg gtttccagat tagcattata
     4321 ggtaatgata tcagctttat ctactatttc aggaatataa gatttataag ccggagaaga
     4381 aaaagcattg gaaaccgcta agatagcatt agcaactatt agaccataca ccattacttt
     4441 atcatctcca ataaaagaaa gtaagaaaca catgatagca cagatagcat ctgttattag
     4501 taaaattttt cttcgttgaa agcgatcagc taaggcgcct ccaaaagggt tgaggacaat
     4561 agaaaccaac aactctacta tctgataaat acctaaaatc ttttgtccta gaccacccat
     4621 cgaagcaatc caagtactat ttccataatc ataaaacaca tcaccaagtt tactgactgc
     4681 tcgactagat agtaatttta taccattaat attcataatt ccctccttag ttcgacatta
     4741 tagcaaacca aaaggagaaa tattaaaaaa tcccataaat gggatttttt ataatcttgt
     4801 taccctatta aagtgttcct gaaaattttc aacatgatgt ttagaaccaa ttgcttggaa
     4861 gcaaaaaata gcttgcttca tgctttgtat tcctcgacta tcccctttag cataggcaag
     4921 aaatcctttt tcatagagta aaataatacg gtgataagca ttccgctcat tatttaaaag
     4981 tgcttcaact ttctctagaa aaaacgttgc ttcttctaga gcgcgccgtt ctattagtgt
     5041 ctctaccaca tttaggaggg ttgcttcgac aacattgcgg tttgtataaa tatctcgata
     5101 atattccttt tgattgataa cctctttaac aagttggcaa atatgccttg tgttgtaata
     5161 ccggtaaaga tttccaatca aaatatagtc actaatttcc caactatcct tttgaaagag
     5221 gtaatccgac aaaaagcaaa ggtcttcttc cgacattttt cttgaggagt cacattcaca
     5281 tatcattcct ctaaaaagaa ctatgtttaa acgacaacgt atatctgaac tatccttttt
     5341 tagtttacct tcttccacag aaatcatctt ttctaaccca gctacgtctc tttgataatg
     5401 atattgagcc atctgagaca ttaaagatat ctgatcagat ttttgatatt tatttactct
     5461 atccataaat tcgctaattg ataaatcaat tgcctctaaa gcccctaaaa attttgttaa
     5521 tgataaatct gattcacccc tctcaaatcg tgataactga gaaacagaaa gttcatttga
     5581 agcaacctgt ttaagagaaa actgtcgatt aagtcgaaat tccctaaaaa tagggacata
     5641 tttaaacatt agtattcctt tctaaccgta atctca
//
LOCUS       DATRNK010000088         5537 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.088, whole genome shotgun sequence.
ACCESSION   DATRNK010000088 DATRNK010000000
VERSION     DATRNK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.088"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..323
                     /locus_tag="VBC52_001871"
     CDS             <1..323
                     /locus_tag="VBC52_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883430.1"
                     /translation="KFADENFFEEFDFVTEERRPLVDQTTKINVKELLDSIIKSPKQF
                     DNGHILHASIREIPGLLTVNSQVSKLFQTRVNEVYLKQIDDLVMYQNIYINLIKPDEL
                     DSFL"
     gene            558..872
                     /locus_tag="VBC52_001872"
     CDS             558..872
                     /locus_tag="VBC52_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883431.1"
                     /translation="MKTRNRKGGYLANTANEYIDSKQAIHCLSVELEPQIRFEEGQPT
                     GEIIAYKAWFSQKGLPPFMVKFENEVTLPAYMVMVQFENLQACEVGFNVYFKADNLKE
                     VK"
     gene            872..1375
                     /locus_tag="VBC52_001873"
     CDS             872..1375
                     /locus_tag="VBC52_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883432.1"
                     /translation="MTKKELSQYLLQSLNMGLGALMQGETSYTNSFDCKIMEEGFLFL
                     PRLPAGYIIDDELYQKIFLIANASLFPRYTLLKQNSAYFMALDTEDIHVQRGLFFPWK
                     EGVSERLIISDLEDFASSQKETLIPIMKNLSLDFNKVNHIAIAGNSGSGKSYALTYFL
                     SLLKGIS"
     gene            1439..1948
                     /locus_tag="VBC52_001874"
     CDS             1439..1948
                     /locus_tag="VBC52_001874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEO6883433.1"
                     /translation="MIHPQKNRSKSDFVSEINENLSSCLELIQQRQEILYENPDHEFD
                     HLTIVIDEVLALSEGVNKVIKESFFSLLSQIALLGRATKIHLLLVSQRFDHNTIPISV
                     REQLNVLIQIGNINKKTTQFLFPDLDPEGIVIPIGKGTGLIQILDNEHPYQVLPLLCP
                     TYYTKKGIL"
     gene            2255..2563
                     /locus_tag="VBC52_001875"
     CDS             2255..2563
                     /locus_tag="VBC52_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883434.1"
                     /translation="MIETILRQEEHSEPVMTIENQTVTRFNPILRSFNRVVSKCTVDA
                     RKDSVSVFLKVPKTQQAQKLLREMEEHIKEEISSRNPKYYFSTPNREKSKLWFTGTKR
                     "
     gene            2698..3264
                     /locus_tag="VBC52_001876"
     CDS             2698..3264
                     /locus_tag="VBC52_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEO6883435.1"
                     /translation="MSKDKRSNKWAFLLYQESVPKNYLEVLEELHIPFVLSPWHDKDV
                     NKETGEFKKAHKHGALFFESLKSYSQVSELLTKHLNTPSHVEVIMSPKGMYDYFIHAE
                     NPDKTLYDINDIESGCGFELDKFLTSNNNDKFLSLVIDIIEEQNFTEFNNLVRYARSE
                     NPQLLALIMNKTYFFAKYLDSRRYSRRK"
     gene            3268..3501
                     /locus_tag="VBC52_001877"
     CDS             3268..3501
                     /locus_tag="VBC52_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEO6883436.1"
                     /translation="MTRTINHKRLMELGFPEHTARNIIKQSKAIAVAKFEETSNSTNN
                     LVKLRKSPFENSRLDLAPTYIVEDLLGFELPFK"
     gene            3519..4700
                     /locus_tag="VBC52_001878"
     CDS             3519..4700
                     /locus_tag="VBC52_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6883437.1"
                     /translation="MANKKYRGVSRDKQGRIYYQTEFKANNITGKRQRKKSYKDQFGN
                     PFRSEKQAYDELCRVRVEFQTKDNNDGYYLSFSQYMEEVYLPYYKQTVQAVTYRTALT
                     HHKMFVEIFGSKRLSEIDVRECEKFRIYLIEKYSPNYAKGVWIRFKHCIGYAERLGYI
                     DEFPCKSLDNPKGKRPDTKFWTLEDLKKVVATFDLRDYEERHRFTAIWLYFFTGLRVS
                     EGLSLIWSDIDFDKKLLHVQSTLEYEGNGVFTRKNPTKTSAGIRWIELDDETLNVLLR
                     WRSIQVENGDDSYVLARFGKPLNKCTLSRILKRQAKKAGVEVITGKGLRHSHDSFLIN
                     VLGKDVLYVSKRSGRVDKATTLNTYSHLYDSKKASGGREISQALHKFGVSSNPTKTPL
                     N"
     gene            complement(4902..5423)
                     /locus_tag="VBC52_001879"
     CDS             complement(4902..5423)
                     /locus_tag="VBC52_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883438.1"
                     /translation="MLNFNYTLPRIDKLQRNIHGTLNDKNIIFGIDYDSLIDIQKDKN
                     VTLPVEFTKSYRILQNGNIFQYPIPSELTFIKFYGHGLGEADYSYFQSIFDSINLYSS
                     DTVLIFYWSCYNENIREQIKSQQVHAVHKLIEKYGDTFNNKNHGRNLFTKLQLENRLI
                     IQEILYNDILNPY"
BASE COUNT         1932 a          917 c          984 g         1704 t
ORIGIN      
        1 agaaatttgc tgatgaaaat ttttttgaag aatttgattt tgttactgaa gaaaggcgtc
       61 cattagttga ccaaaccact aaaataaatg taaaagagtt gttagattct attataaagt
      121 ccccaaaaca atttgataat ggtcatattt tacatgctag tataagagaa attccaggac
      181 tattaacagt caatagtcag gtatcaaaat tatttcaaac aagagttaat gaagtttact
      241 taaaacaaat agatgattta gtcatgtatc aaaacatata tatcaactta atcaaaccag
      301 atgaactaga ttcattttta taaccaaatt attaaacgga cagttacagg agtacttgtc
      361 cgttttcatt ttgtccataa aagaagtaga aactgagaac taaagtaaga atttagaaga
      421 atgtattctc cgatatacta aagacatcaa agcacagcaa atctttggta gtcaacgctc
      481 aagagagttg caaatctgtt gagtcacttt tcaggaagtt gctgattcct gaacgggttt
      541 ttaatggagg ataaaacatg aagacaagaa atcgtaaagg tggttattta gcgaatactg
      601 caaatgagta catcgactct aaacaggcaa ttcattgctt gagtgtagaa cttgaaccgc
      661 aaattaggtt tgaagagggt cagcctactg gggagattat cgcttataag gcttggttct
      721 ctcaaaaagg gcttccgcct tttatggtga agtttgaaaa tgaagtgaca ctaccagcat
      781 atatggtaat ggtgcaattt gagaatcttc aagcttgtga ggttgggttc aatgtttatt
      841 tcaaggcaga caatctcaag gaggtcaaat aatgaccaag aaggaattgt cacagtatct
      901 gcttcaaagt ctcaatatgg gacttggagc tcttatgcaa ggggaaacaa gttataccaa
      961 tagttttgac tgcaaaatta tggaagaagg atttctcttt cttccccgct taccagcggg
     1021 atatattatt gatgatgaac tctatcaaaa aatattccta atcgccaatg cttcactctt
     1081 ccctcgttac acactattga aacaaaattc tgcctatttc atggcacttg atacagaaga
     1141 tattcatgtt caacgtgggc tatttttccc atggaaagaa ggcgtatctg aacgcttaat
     1201 catttctgac ttggaagatt tcgcaagctc tcaaaaggaa actctgattc ctatcatgaa
     1261 aaatctatct cttgatttta acaaggtcaa tcatattgcc attgcaggaa attcaggatc
     1321 aggtaaatct tatgctctga cctactttct gagtctctta aaagggattt cttaactgat
     1381 tatcgttgac cctaaatatg atactccaag cagatgggca agagaaaatg gtattgcagt
     1441 gattcaccct caaaagaatc gctctaagtc tgattttgta tcggaaatca atgagaatct
     1501 aagttcgtgc ttagagctta tccaacaacg ccaagaaatt ctctatgaaa atcctgacca
     1561 cgaatttgac catctaacca ttgtcatcga tgaagttttg gcactttcag aaggagtgaa
     1621 caaagttatc aaagaatcct ttttctcact actttctcaa atagctttac ttggtagagc
     1681 tactaaaatt cacttactac tggtatcaca acgatttgac cataacacga ttccgatttc
     1741 cgtacgtgaa cagttaaatg tcttgattca aattggcaac atcaataaaa aaacgactca
     1801 gttcctcttt cctgacttag accctgaagg tattgtcatt ccaataggaa aaggaactgg
     1861 cttaattcaa atacttgata acgaacatcc ttatcaagta ttgccactcc tttgtccaac
     1921 ctactatact aagaaaggta ttttataaaa tgaaaagaag tttcttccaa aaaacgttta
     1981 acctgaatct gaatttgctt ttctttggtt tcattgcctt cttaatcagt tatggattcc
     2041 atgtactagg caatcaaatc aagacaagtt ctttcttgag ctctcaatca gtctttgcag
     2101 atattcaaac taagttacaa gtggtagacc agtatactca gtcaatagca tatttgttta
     2161 ttctgattgc cattattcta accttgactg aactctgtca gcgaattatg aaggactcaa
     2221 tatggaacta ttttaagtct gtttatcaaa ccttatgatt gagacaattc ttagacagga
     2281 agaacattct gaacctgtta tgacaataga aaatcaaacg gtcactcgct ttaatcctat
     2341 cctaagaagt ttcaatcggg ttgtttctaa gtgcacggtg gatgcccgaa aggattctgt
     2401 ttctgtcttt ctaaaagtcc ctaaaacgca acaggcacaa aaactcttgc gagaaatgga
     2461 agaacatatc aaggaagaaa tttcgagccg aaatcctaag tattatttct ctaccccaaa
     2521 tcgggagaaa agtaagctct ggtttacagg tactaaacgg taagggaaag agtggtgctt
     2581 ggctcacgaa gtgaaagcca aaagcaccac tctttcttat aactgttttt aacatgtgta
     2641 atggggttac tacgcccccc cattacacca ataatcaata atcacgaaag gaagtatatg
     2701 tcaaaagaca aacgctcgaa taaatgggct tttctgctct atcaggaaag tgtgcctaag
     2761 aactatttag aggtgcttga agagttacat atcccctttg ttttaagtcc gtggcatgat
     2821 aaagacgtaa acaaggaaac aggggaattc aaaaaagctc acaaacatgg ggctctcttc
     2881 tttgaatctc tcaaaagcta ttcacaagtc tctgaattat tgacgaagca cctaaacaca
     2941 ccaagtcatg ttgaagttat catgtcacct aaaggaatgt atgactactt tattcatgcg
     3001 gagaatccag ataaaacact ctatgatatc aatgacattg aatcaggttg tggatttgag
     3061 ttagataaat ttctcaccag caataacaat gataaatttt tatctctggt aattgacatt
     3121 atcgaagaac aaaatttcac agagtttaat aatctggtta gatatgcgag aagtgaaaat
     3181 cctcaactgc ttgccttgat aatgaataaa acctatttct ttgcaaaata tctggattcg
     3241 agaagatatt caaggaggaa gtaacgattg acaagaacaa taaatcataa aagattaatg
     3301 gagcttggct ttccagaaca tactgcaaga aatattatta aacaatcaaa agcgatagca
     3361 gtagcaaagt ttgaagaaac tagtaattct acaaataatt tggtaaaatt gaggaaatcg
     3421 ccctttgaaa attctagact agaccttgca cctacctata ttgtagaaga tttgctaggc
     3481 ttcgagttac cttttaagta aagaaaggaa acttgaaaat ggcaaataaa aaatatcgtg
     3541 gtgtatcacg tgataaacaa ggaagaatat attatcaaac ggagttcaag gctaataata
     3601 taactggtaa acgccaacgc aaaaagagtt ataaagacca atttggaaat ccttttcgtt
     3661 ccgaaaagca agcatatgat gaactttgta gagtgagagt tgaattccaa acgaaagata
     3721 acaatgatgg ttattatcta agtttttctc aatacatgga agaagtgtat ctaccttatt
     3781 acaaacaaac ggttcaagcg gtcacttatc ggactgctct aactcatcac aaaatgtttg
     3841 ttgaaatctt tggtagtaaa agactttctg aaattgatgt tcgagagtgc gaaaaattca
     3901 ggatatatct gattgaaaag tattcaccaa attatgctaa aggtgtatgg attcgattta
     3961 agcattgtat tgggtacgca gagaggctgg gatatattga tgaattccct tgcaaatctc
     4021 ttgataatcc gaaagggaaa agaccagata caaagttttg gactctagaa gacttaaaaa
     4081 aagtagtcgc tacctttgac ctacgggatt atgaagaaag gcatagattt actgccatat
     4141 ggctctattt ctttacgggt cttcgtgtat cagagggttt aagtttaatt tggtctgata
     4201 ttgatttcga caaaaagctc ttacatgttc aaagtacact tgagtatgaa ggaaacggag
     4261 tctttactcg caaaaatcca actaagactt cagctggtat aaggtggatt gagttagatg
     4321 atgaaactct aaatgttctt ctaagatggc gttcaattca agtggaaaat ggtgatgaca
     4381 gttatgtatt ggctagattc ggaaaaccac tcaataaatg cacattaagt agaattctaa
     4441 aacgccaagc taaaaaagca ggagtagaag taatcactgg aaaagggttg cgacattctc
     4501 acgacagttt tttgataaac gtactaggaa aagatgttct gtatgtttct aaacgttctg
     4561 gacgagttga taaggcaact accttgaata cctattctca tttgtatgat agcaaaaaag
     4621 ctagtggtgg aagagagata tcccaagcat tacataagtt tggagtatca tcaaacccca
     4681 ccaaaacccc actaaactaa ataagagcaa gaaaaacccg attatatcaa tgtttataag
     4741 cattgtgtaa ctgttactct taaataatca tctattggtt gtttaaaggt gaaagcctag
     4801 ttttaaagtc ttttcgttaa agttaaggcg attaaaaaag gaaagattca attgaatctt
     4861 ccccacaaac acccccacaa tattttgtgg gggtgttttg tttaatatgg atttagaata
     4921 tcattataaa gtatttcttg aataattaaa cgattttcta attgaagttt tgtgaataaa
     4981 tttctgccat gattcttatt attaaaagta tctccatatt tttcaattaa tttatgtact
     5041 gcatgaactt gttgagattt aatttgttcc ctaatattct cgttataaca actccaataa
     5101 aaaattagta cagtgtcact ggaataaaga ttaattgagt caaaaattga ttgaaaataa
     5161 gaatagtctg cttcacccag accatgacca taaaatttta taaaagttaa ttcagaggga
     5221 attggatatt gaaatatatt tccgttctgt aatatcctgt atgatttggt aaattcaaca
     5281 ggcagggtaa catttttatc tttttgtata tcaattaaag aatcataatc tattccaaaa
     5341 ataatatttt tatcattcag ggttccatga atattacgtt gtaatttatc tattctaggt
     5401 aatgtataat taaaattcaa tattgatgtt tcaacattaa attcattatt actttctaag
     5461 tcataaatgt agctaagaaa tccttcatag gaattgaaac aattttcgtt atgaaaatca
     5521 tgtagtgcaa caaaaga
//
LOCUS       DATRNK010000089         5462 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.089, whole genome shotgun sequence.
ACCESSION   DATRNK010000089 DATRNK010000000
VERSION     DATRNK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.089"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            181..1224
                     /locus_tag="VBC52_001880"
     CDS             181..1224
                     /locus_tag="VBC52_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO6883439.1"
                     /translation="MNRKIIGLTLLSLSVLTLTACGNRSDKSANKSDIKVAMVTNQGG
                     VDDKSFNQSAWEGLQKWGKKKGLTKGNGFDYFQSSNESDHANNLDTAASSGYNLIFGI
                     GFGLHDTIEKVSENNKDVKYVIVDDIIKGKENVASVTFADNEAAYLAGVAAAKTTKTK
                     TVGFIGGMEGVVVKRFEAGFKAGVKSIDPAIKVAVSYAGSFTDAAKGKTIAATQYATG
                     VDVIYQAAGGTGAGIFSEAKTENETRKESNKVWVIGVDRDQSQEGNYVSKDGKKANFV
                     LASTIKEVGKSLQSVAELTEKKQYPGGKVTVFGLKDSGVDIKEHQLSSEGSVAVKKAK
                     EDIVSGKIQVPMK"
     gene            complement(1315..2214)
                     /gene="galU"
                     /locus_tag="VBC52_001881"
     CDS             complement(1315..2214)
                     /gene="galU"
                     /locus_tag="VBC52_001881"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEO6883440.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKNELLKLVDETTGIRLHFIRQ
                     SHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNNKVIPLTKQLINDFEATHASTIA
                     VMEVPHEDVSAYGVIAPQGEGVNGLYSVNTFVEKPSPEEAPSNLAIIGRYLLTPEIFN
                     ILETQKPGAGNEIQLTDAIDTLNKTQRVFARKFTGDRYDVGDKFGFMKTSIDYALQHP
                     QVKDDLKKYIIDLGKSLEKTSKK"
     gene            complement(2251..3267)
                     /locus_tag="VBC52_001882"
     CDS             complement(2251..3267)
                     /locus_tag="VBC52_001882"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO6883441.1"
                     /translation="MTKQKIAVLGPGSWGTALAQVLNDNGHEVRLWGNVVEQIEEINT
                     NHTNQRYFKDITLDSKIKAYTNLEEAINNVDSILFVVPTKVTRLVAKQVANLLKHKVV
                     LMHASKGLEPGTHERLSTILEEEISEQYRSDIVVVSGPSHAEEAIVRDITLITAASKD
                     IEAAKYVQKLFSNHYFRLYTNTDVVGVETAGALKNIIAVGAGALHGLGYGDNAKAAII
                     TRGLAEITRLGVQLGADPLTFSGLSGVGDLIVTGTSVHSRNWRAGDALGRGEKLEDIE
                     KNMGMVIEGISTTKVAYEIAQNLNVYMPITEAIYKSIYEGANIKDSILDMMSNEFRSE
                     NEWH"
     gene            3437..3766
                     /gene="rnpA"
                     /locus_tag="VBC52_001883"
     CDS             3437..3766
                     /gene="rnpA"
                     /locus_tag="VBC52_001883"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEO6883442.1"
                     /translation="MKKTYRVKSDKDFQMIFSRGKNVANRKFVIYYLEKEQKHFRVGI
                     SVSKKLGNAVVRNAIKRKIRHVLLSQKTALQDYDFVVIARKGVEELDYQALEKNLIHV
                     LKIAGLI"
     gene            3779..4594
                     /locus_tag="VBC52_001884"
     CDS             3779..4594
                     /locus_tag="VBC52_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO6883443.1"
                     /translation="MKKKLKTFSLILLTGSLLVACGRGEVSSHSATLWEQIVYAFAKS
                     IQWLSFNHSIGLGIILFTLIIRAIMMPLYNMQMKSSQKMQEIQPRLKELQKKYPGKDP
                     DNRLKLNDEMQSMYKAEGVNPYASVLPLLIQLPVLWALFQALTRVSFLKVGTFLSLEL
                     SQPDPYYILPVLAALFTFLSTWLTNKAAVEKNIALTLMTYVMPFIILVTSFNFASGVV
                     LYWTVSNAFQVFQILLLNNPYKIIKVREEAVRVAHEKEQRVKRAKRKASKKRK"
     gene            4602..5423
                     /locus_tag="VBC52_001885"
     CDS             4602..5423
                     /locus_tag="VBC52_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEO6883444.1"
                     /translation="MVLFTGATVEEAIEKGLQELNISRLRAHIKVVSREKKGFLGFGK
                     KPAKVEIEGITDEVTDINESVALKNIKNVPSSVDVVEEYIEEVDETLEKEDVSQPELP
                     KIDDKNVVTTSEAIEKIDLLPNIEVAAAQVTKYVENIIYEMDLDATIETTTSKRQINL
                     QIETPEAGRIIGYHGKVLKSLQLLAQNYLHDRFSKSFSVSINVHDYVEHRTETLIDFS
                     KKIARRVLETNEPYHMDPMSNSERKTVHKTIATIEGVESYSEGNDPNRFVVVTKK"
BASE COUNT         1847 a          876 c         1027 g         1712 t
ORIGIN      
        1 gatatttatt attgtatcga tcagcttaat aggtctatcg ttggtataat tctacggcta
       61 tagcctctaa aaccaaactt tctctgggaa aaatagaata aagtttgcta aatgattttt
      121 ttatgttatg atagaaatct gtttttatgt gactataact tattaggagg ttcattctcg
      181 gtgaacagaa aaattattgg cttaactcta ctatctttat ctgtactaac attaacagca
      241 tgtggtaatc gttcagataa gtctgcaaat aaatcagata tcaaagttgc gatggtaact
      301 aatcaaggtg gagtagacga taaatcgttt aatcaatcag cttgggaagg gctacaaaag
      361 tggggaaaaa agaaggggtt gacaaaaggt aatggctttg attatttcca gtcatctaat
      421 gaatcagatc atgctaataa ccttgatacg gcagcctcta gtggttataa tttaattttt
      481 ggtattggat ttggtcttca tgatactatt gaaaaagtat cagaaaataa taaagatgtt
      541 aaatacgtta ttgtagatga tatcattaaa ggtaaagaaa atgtagctag tgttactttt
      601 gcagacaatg aagctgctta cttagctgga gttgctgcag ctaagacaac taagacaaaa
      661 acagttggtt ttatcggagg gatggaaggc gtcgttgtta agcgatttga agcaggattt
      721 aaggctggag taaaatcaat tgatccggct attaaagttg cggtatctta tgcaggctca
      781 tttacagatg cagctaaagg taaaacgatt gctgccacac agtatgcaac aggtgttgat
      841 gttatttacc aggctgctgg tggtacagga gcaggaattt ttagtgaggc taaaactgaa
      901 aatgagaccc gcaaggaatc taacaaagtc tgggtaattg gtgttgacag agatcaaagt
      961 caagaaggca attatgtttc aaaagatggt aagaaagcaa actttgttct ggcatctact
     1021 ataaaagagg tggggaaatc tctccaatct gtagcagaat taacagagaa aaaacaatat
     1081 ccaggaggga aagtaacagt ttttggtcta aaggatagtg gtgtagatat taaagagcat
     1141 caattgtcta gtgagggatc tgttgctgtc aaaaaagcta aagaggatat tgtttcaggg
     1201 aaaatacaag ttcctatgaa atgataaaag tgtggaggaa caataagttc ttcgtcaagt
     1261 acaataaaaa agtttcctat ttaggaaact tttttattgt acttgactca tattttactt
     1321 cttagatgtt ttttctagag attttcctaa atcaatgata tatttcttca agtcatcttt
     1381 aacttgtggg tgttgaagag cataatcaat agatgttttc ataaaaccaa acttgtcacc
     1441 aacgtcgtaa cggtctcctg taaattttct agcaaaaaca cgttgcgttt tatttaaagt
     1501 atcgatagca tcggttagtt gaatctcatt tcctgcacca ggtttttgag tttctaaaat
     1561 attaaaaatt tctggagtta acaagtagcg accaatgatt gccaaattac ttggagcctc
     1621 ttcgggagaa ggtttttcaa cgaaagtgtt aacgctatat agaccattaa ctccttctcc
     1681 ttgtggagca ataacaccat aagcagaaac atcttcatgt ggaacttcca taacagcaat
     1741 tgttgatgca tgagtagctt caaagtcatt aattaattgt ttggtgagag gaataacttt
     1801 attattggta atatccatta gatcatcacc tagcataaca acaaaaggtt cattaccaac
     1861 gaaggctttt gcttgtagca cagcatctcc aagcccacga ggatgacttt gtcgaataaa
     1921 atggagtcga atgccagttg tttcatcaac aagtttgaga agttcgtttt tccctttctc
     1981 ttttaggtta tattctaatt caaaatttga atcaaagtgg tcttctatag aacgttttga
     2041 tttaccagtt acaactaaaa tatcttcaat tccagatttt agtgcttctt caacaataaa
     2101 ttgaatagtt ggtttatcaa cgattggaag catttcttta gctaaagctt tagtagcagg
     2161 taaaaaacgt gttcctaaac cagcggcagg gataactgct tttctgactt tcatatatag
     2221 ctcctttaaa tagtattaat ataaacgata ttagtgccat tcattctctg aacgaaattc
     2281 attggacatc atatctaaaa tgctatcttt gatattagcc ccttcatata ttgatttata
     2341 aatagcttct gtaatgggca tgtaaacatt taagttttgg gcgatttcgt aagcaacttt
     2401 agttgttgag ataccttcaa taaccatccc catatttttt tcaatatctt caagtttctc
     2461 accacgtcct aaagcatctc ccgctctcca gttgcgagaa tgaacagatg taccagtcac
     2521 aattaaatct ccaactccgg ataaaccact gaaagtgaga gggtcggccc ctaattgtac
     2581 accaagacgt gttatctcag ctaaaccacg cgtaataata gctgctttag cattatcacc
     2641 ataaccaaga ccgtgtagtg ctccagcacc aacagcaata atatttttta gagcaccagc
     2701 tgtttctact cctacgacat cagtgttagt ataaagtcta aaataatgat tactaaacaa
     2761 tttttggaca tacttagctg cttcaatatc ttttgaagca gcagtaatca aagtaatatc
     2821 acgaacgatg gcttcttcag catgactggg acctgaaaca acaacgatat cactacgata
     2881 ttgttcagag atttcttctt ctaaaattgt agaaagtcgt tcatgagtac caggttctag
     2941 tcccttagaa gcatgcataa gaacaacttt atgctttaaa agattagcca cttgtttagc
     3001 aacaagtctt gtaactttag ttgggacgac gaataaaatg ctgtcaacat tattaatcgc
     3061 ttcctcaaga ttagtgtagg cttttatttt ggaatctaag gtaatatctt tgaagtaacg
     3121 ttgattagta tggttagtat taatctcttc tatttgttca accacgtttc cccataagcg
     3181 tacctcatgt ccattgtcat ttaatacttg tgcaagagca gtcccccatg aaccaggtcc
     3241 aagaacggca attttttgtt ttgtcatctt attccttctt tctaataata aggcatatag
     3301 cgctaacatt atatcatact ctaatacact ttgtcttgtt tagtctgttg tcaatgatag
     3361 taacattaga aaagaattga gtcttttagg ttatttatgg tataataaaa ccaatttaga
     3421 taaatggagc actattttga aaaaaaccta tcgggtaaaa agtgataagg attttcaaat
     3481 gattttcagc agaggtaaaa atgttgctaa tcgaaaattt gttatctact atttagaaaa
     3541 agaacaaaaa cactttcgtg tgggaatctc tgttagtaag aaattaggaa atgctgttgt
     3601 tcgtaacgct attaaaagaa aaattaggca tgttttactc tctcagaaaa cagcgctgca
     3661 agattatgat tttgtcgtga ttgcgcgtaa aggtgtagag gaattggact atcaagcatt
     3721 agaaaaaaat cttatacacg ttttaaaaat agcaggactc atctagaaag gtataaaatt
     3781 gaagaaaaaa cttaaaacat ttagcttaat attgctgaca ggctctttat tggttgcttg
     3841 cggacgagga gaagtatcta gtcattcagc cacattatgg gagcaaatag tttatgcatt
     3901 tgctaaatca atacaatggc tctcctttaa ccattcaatt ggtttgggaa taatcttatt
     3961 cactctaata atcagagcga ttatgatgcc gctttataat atgcaaatga aatccagcca
     4021 aaagatgcag gaaattcagc caaggctcaa ggaattacaa aaaaaatatc cagggaaaga
     4081 tcctgataac cgtttaaagc taaatgatga aatgcaatct atgtataaag ctgagggagt
     4141 taacccatat gcaagtgtat tacctcttct aattcagtta cctgtcctat gggctctttt
     4201 ccaagctttg actagggttt ctttcttaaa agtaggaaca tttttgagcc tagaattatc
     4261 acagccggac ccatattata ttttacctgt actggcggcc ttatttacct ttttatcaac
     4321 ttggttaact aacaaagcag ctgttgaaaa aaatattgct ttaactctta tgacttatgt
     4381 tatgccgttt atcatattgg taacaagctt taactttgct agtggtgttg ttttatactg
     4441 gacggtgtct aatgcttttc aagtatttca aattctatta ttgaacaacc catacaagat
     4501 tataaaggtt cgagaagaag cagtaagagt ggcacatgaa aaagaacaaa gagttaaacg
     4561 tgctaagaga aaagcaagta agaaaagaaa gtaggaaaac catggtatta tttacaggag
     4621 cgactgtaga agaagctatt gaaaaaggac tacaagagtt aaacatttca cgattgaggg
     4681 cacatataaa agttgtatca cgtgagaaaa aaggtttttt gggctttggt aaaaagccag
     4741 caaaagttga aatagaaggt atcaccgacg aagtaactga tattaatgaa tcggtagcgt
     4801 taaaaaatat taagaatgta ccgtctagcg tagatgtggt ggaagaatat atagaggagg
     4861 ttgatgaaac gcttgaaaaa gaagacgtat cacaaccaga acttccaaaa attgatgata
     4921 aaaacgttgt aactacaagt gaagctattg aaaaaattga cctacttcct aatattgaag
     4981 tagcagctgc tcaggtgact aaatacgttg aaaatatcat ctatgagatg gatttagatg
     5041 caacaataga aactacgaca agtaagcgac aaataaatct ccaaatagaa acgccagaag
     5101 ctgggcgcat cattggttat catggaaaag tattaaaatc gttgcagttg ttagcacaaa
     5161 attatcttca tgaccgcttt tctaagagtt tttcagtatc cattaatgtc catgactacg
     5221 ttgagcatcg tacagaaact ttaattgatt tttctaaaaa aatagctagg cgtgttttag
     5281 agactaatga accttaccat atggatccta tgagtaacag tgagcgaaaa actgttcata
     5341 aaacaattgc aactatagag ggtgttgaaa gttattctga aggtaacgat cctaatagat
     5401 ttgtagtagt tactaagaaa taaaaagagg tgagacctct ttttttcttg cgctctatct
     5461 ac
//
LOCUS       DATRNK010000090         5286 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.090, whole genome shotgun sequence.
ACCESSION   DATRNK010000090 DATRNK010000000
VERSION     DATRNK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.090"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..76
                     /locus_tag="VBC52_001886"
     CDS             <1..76
                     /locus_tag="VBC52_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEO6883445.1"
                     /translation="NNSKEELFNRKVIEKIIRTSIENY"
     gene            86..1072
                     /gene="dhaQ"
                     /locus_tag="VBC52_001887"
     CDS             86..1072
                     /gene="dhaQ"
                     /locus_tag="VBC52_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DhaKLM operon coactivator DhaQ"
                     /protein_id="HEO6883446.1"
                     /translation="MRKTEHHKMEYVFETIKAITKTNPKVEQIKDLPAIHYLGQSEQV
                     IPILSGGGSGHEPAHFGYVGEGMLSAAISGPIFVPPCASDILETIRFINRGKGVFVII
                     KNFEADLEEFSQAIEQARQEGIPIKYIVSHDDISVETSNFKIRHRGVAGTVLLHKIIG
                     QAALEGASLDELEQLGLSLTTSMATLGVASKSATILGQHQPVFDLEEGYISFGIGIHG
                     EPGYRTMPFVSMEHLANELVNKLKMKLRWQDGEAFILLINNLGGSSKMEELLFTNAVM
                     EFLALDDLQLPFIKTGHLITSLDMAGLSVTLCRVKDSRWIDYLKHKTDARAW"
     gene            1190..1906
                     /locus_tag="VBC52_001888"
     CDS             1190..1906
                     /locus_tag="VBC52_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6883447.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKQGEPDPESN
                     SALKFVLDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADTVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            2061..2750
                     /locus_tag="VBC52_001889"
     CDS             2061..2750
                     /locus_tag="VBC52_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEO6883448.1"
                     /translation="MLTKPIIGITGNEREMSDIPGYYYDSVSRHISEGVKNAGGLPVI
                     LPISEAESAKAYVEMIDKLIISGGQNVLPSYYGEEKIIESDDYSLARDIFEFALVEEA
                     LKQNKPIFAICRGMQLVNVALGGTLNQSIDNHYQEPYIGFAHYLNVEKGSFLEGFISG
                     DFKINSLHRQSVKLLAEGLIVSARDPRDGTVEAYESRTEQCIIGVQWHPELMLHQIEN
                     QTLFGYFVNET"
     gene            2852..3685
                     /locus_tag="VBC52_001890"
     CDS             2852..3685
                     /locus_tag="VBC52_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6883449.1"
                     /translation="MTIKKVLSVTGIILVTVASLAACSSKSHTTKTGKKEVNFATVGT
                     TAPFSYVKDGKLTGFDIEVAKAVFKGSDNYKVTFKKTEWSSVFTGIDSGKFQMGGNNI
                     SYSSERSQKYLFSYPIGSTPSVLAVPKNSNIKAYNDISGHKTQVVQGTTTAKQLENFN
                     KEHQKNPVTLKYTNENITQILTNLSDGKADFKLFDGPTVNAIIKNQGLTNLKTIPLTM
                     RDQPYIYFIFGQDQKDLQKYVNNRLKQLRKDGTLSKIAKEYLGGDYVPNEKDLVTPKE
                     K"
     gene            3826..4650
                     /locus_tag="VBC52_001891"
     CDS             3826..4650
                     /locus_tag="VBC52_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO6883450.1"
                     /translation="MKLKHIVLGLALTTLLGVTFSNQEVSASSTSSKVVKVGVMTFSD
                     TEKARWDKIEKLVGDKAKIKFTEFTDYTQPNQATANKDVDINAFQHYNFLENWNKENK
                     KNLIPLEKTYLAPIRIYSEKVKSLKKLKKGATIAIPNDATNGSRALYVLQSAGLIKLN
                     VSGKKVATVANITSNKKDINIQELDASQTPRALKDVDAAIINNTYIEQANLKPSDAIF
                     VEKSDKNSKQWINIIAGRKNWKKQKNAKAIQAILDAYHTDEVKKVIKDTSADIPQW"
     gene            4784..>5286
                     /locus_tag="VBC52_001892"
     CDS             4784..>5286
                     /locus_tag="VBC52_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEO6883451.1"
                     /translation="MTLTNEENQIQKFLSDTIVQDDINILRDLIAIKSIFAQKVGLND
                     LSSYLGEVFRKAGAEVIIDDSYSAPFIVANFKSSKVDAKRIIFYNHYDTVPADEVEQW
                     TEDPFTLSLRYGKMYGRGVDDDKGHITARLSAVKKYLSRHKGELPLDITFIVEGAEES
                     ASVGLDY"
BASE COUNT         1886 a          784 c         1043 g         1573 t
ORIGIN      
        1 aaataactca aaagaagaat tatttaatag gaaagtcatt gaaaaaatta tcagaacatc
       61 aatagaaaat tattaggaca aggatatgag aaaaacagaa catcataaaa tggaatatgt
      121 ttttgaaaca ataaaagcaa tcactaaaac taatcctaaa gtagagcaaa taaaagattt
      181 gcccgctatt cattatttag ggcaatccga gcaagtaatt ccgattttat caggaggagg
      241 cagcggacat gagccggctc actttggata tgttggtgag ggaatgctat cggcagcgat
      301 tagtggtcct atttttgtac caccatgtgc tagtgatatt ctagagacta ttagatttat
      361 caatcgaggc aaaggtgtct ttgttattat caaaaatttt gaagctgatt tggaagaatt
      421 ttcccaagcg attgaacagg ctcgtcaaga aggcattcca atcaaataca tcgtttctca
      481 tgatgacatc tctgttgaaa catcaaattt taaaattcgt catcgaggcg ttgctggtac
      541 tgtgttgcta cataaaatta ttggacaagc tgctttagaa ggagcaagct tagatgaatt
      601 agaacagcta ggactatctt tgacaacttc aatggctact ttaggtgtag caagtaagtc
      661 agccactatt ttaggtcagc atcaacctgt ttttgattta gaagaaggtt acatctcatt
      721 tggaatcggt attcatggtg agccaggata ccggacaatg cctttcgtgt ccatggaaca
      781 tttggcaaac gaactagtta acaaattgaa aatgaaatta cgttggcaag atggagaagc
      841 attcattcta ttgataaata atcttggagg aagttctaaa atggaggaat tactttttac
      901 aaatgctgtc atggaatttc tggctttaga cgatttacaa ttacctttta ttaaaacagg
      961 ccacctaatt acaagtttag atatggctgg tttatcagtt accttgtgtc gtgtaaagga
     1021 tagtagatgg attgattact taaaacataa gacagatgct agagcttggt gatagaattt
     1081 ccattttacc aattgctatc aaagtgctat aataatagag ttaaaattaa gattgcttgc
     1141 caatatgagc cagcaggtta ttaaaaatga aatcaaagga gattttccaa tgggacgtaa
     1201 gtgggccaat attgttgcca aaaagactgc taaagatggt gctaactcaa aagtatacgc
     1261 taaattcggt gttgaaatat atgttgctgc aaagcaaggt gaaccagacc ccgagtcaaa
     1321 ctcagctcta aaattcgttt tggaccgtgc taagcaagca caagttccaa agcatgttat
     1381 tgataaagcg attgataaag ccaaaggaaa cacagatgaa acttttgtag agggacgcta
     1441 tgaaggtttt ggtccaaatg gttcaatgat tattgtggat actttgacat caaatgttaa
     1501 ccgtacggca gcaaatgtac gtactgctta cggtaagaac ggtggcaata tgggagcttc
     1561 aggatcggta tcctacttat ttgataaaaa aggtgtcatc gtttttgctg gtgatgatgc
     1621 tgacactgtc ttcgaacaat tacttgaagc ggatgtagac gtagatgatg ttgaagcaga
     1681 agagggaacc ataacagttt ataccgcccc aacagatctt cataaaggta tccaagcact
     1741 tcgcgataat ggtgtagaag aattccaagt tactgaactt gaaatgattc ctcaatcaga
     1801 agtagtattg gaaggtgatg accttgaaac ttttgaaaag cttattgacg cactcgaatc
     1861 agatgatgat gttcaaaaag tttatcataa tgtagcagat ttctaaaaaa taaaaccgta
     1921 gtattaatac tatggttttt atttttgtga gagttgatag gtattgttaa agaagtgata
     1981 ggtaaaaaat attattcaaa aagttttttt aggtgtaaaa tgataattat tagacagtta
     2041 ttggttgatg agggagcaaa atgttaacaa agcctattat tggtattaca ggaaatgaaa
     2101 gagaaatgtc agatattcca ggttattatt atgattcggt atctaggcat atttcggaag
     2161 gagttaaaaa cgcaggcggg ttaccagtta tcctaccaat aagtgaagca gaaagtgcca
     2221 aagcttatgt tgagatgata gataagctga tcatatctgg tggccaaaat gttttaccat
     2281 cctattatgg tgaagagaag ataattgaga gtgatgatta ttcacttgcg cgtgatattt
     2341 ttgaatttgc attagtcgaa gaagcgttga aacaaaataa accaatcttt gcgatttgcc
     2401 gtggcatgca gttagtaaat gtggcgcttg gtggtacact taatcaatct attgataatc
     2461 actatcaaga gccttacatt ggatttgctc attatttaaa tgtggaaaaa gggagttttc
     2521 tagaaggctt tatatcaggt gattttaaga tcaattctct tcatagacaa agtgttaaac
     2581 tgttagctga aggtctgata gttagtgcta gagatcctag agatgggaca gtagaagctt
     2641 atgaaagtcg aacagaacaa tgtattatag gtgttcaatg gcatccagaa ttaatgcttc
     2701 accaaattga aaaccaaacc ttatttggtt attttgttaa tgaaacgtag catccatata
     2761 atagcaaaaa ctctcagacg atagaagata agtgttagtt aaaaacttaa agatgttatg
     2821 atgtcgtgta caattaaaga ggagaaaaaa gatgacaata aaaaaagtgt taagtgtaac
     2881 aggaattatt ttagtgacag tagcgtctct agctgcttgt agctcaaaat ctcatactac
     2941 taagacgggc aaaaaagaag ttaattttgc aactgttgga acaacggcac ctttttctta
     3001 tgtgaaggat gggaaactga ctggctttga tattgaagta gccaaagctg tttttaaagg
     3061 ttcagataac tataaagtca cttttaaaaa aacagaatgg tcatcggtat ttaccggcat
     3121 agattcagga aagtttcaaa tgggtggaaa taatatttct tattcatcag agagatctca
     3181 aaaatattta ttttcatacc caataggctc tactccttca gttttagcag ttcctaagaa
     3241 tagtaatatc aaagcttata atgatattag tggtcataaa acacaggttg tccaaggaac
     3301 gacaactgcc aagcaattag aaaatttcaa taaagagcat cagaaaaatc ctgttactct
     3361 aaaatatact aatgaaaata ttacacagat tctaacgaat ttgagtgatg gaaaagctga
     3421 ttttaaactt tttgacggac caactgttaa cgctatcata aagaatcaag gtctcacgaa
     3481 tttaaaaacc atccctttga ctatgagaga tcagccttat atttatttca tctttggtca
     3541 ggatcaaaaa gacttgcaaa agtatgtgaa taatcgcctt aaacaattac gtaaagatgg
     3601 gacactttca aaaatagcta aagaatacct tggaggtgat tacgtaccaa atgagaaaga
     3661 tctcgttact cctaaggaga agtagtaata aaaactaagg agatgtcaat ccttagtttt
     3721 ttgttatata gtttaaaact ataacaagag acagtttata acaatttgat tctaataact
     3781 tcctatggga taatatcttt atcacaaaaa ttgagggaat ttattatgaa gttaaaacat
     3841 attgtcttag gattagcctt aacaacactt ttaggagtca catttagtaa tcaagaagtt
     3901 tcagcaagct caacttcaag taaagttgtt aaagttggtg ttatgacctt ttctgacact
     3961 gaaaaagcac gttgggataa aattgaaaag ctagtaggtg ataaagctaa aatcaaattt
     4021 acagaattta cagattatac acaaccaaat caagcgacag ccaataagga tgtggatatt
     4081 aatgcctttc aacattacaa tttcttagaa aactggaata aggaaaataa gaaaaactta
     4141 attccacttg aaaagactta cttagctcca attcgtatct attctgagaa ggtaaaatct
     4201 cttaaaaaat tgaaaaaagg agccactatt gcaattccaa atgatgcaac aaatggtagc
     4261 cgtgcattgt atgtccttca gtcagcaggt ttaatcaaat tgaatgtttc tggtaagaag
     4321 gttgcaacag ttgctaatat cacatctaat aaaaaggata ttaatattca ggagttagat
     4381 gcgagtcaaa caccacgtgc actcaaagat gtagatgcag ctattattaa taatacatac
     4441 attgagcaag ctaatttaaa accttcagat gctatctttg ttgagaaatc agataaaaat
     4501 tcaaaacaat ggattaatat cattgcggga cgtaaaaatt ggaaaaagca aaagaacgct
     4561 aaagctatcc aagctatctt ggatgcttat cacacagatg aagtgaaaaa agttatcaaa
     4621 gatacttcag ctgatattcc acaatggtaa cgtgaattga ctgtataagt ttaacaggga
     4681 atcctaatag gatacaactt atcagccata tagccataaa gggcctggga cgaatacaat
     4741 gtgccaggcc cttggattgt ttttgaagga aggtggatga aagatgactt taacaaacga
     4801 agaaaatcaa attcaaaagt ttctatctga tactattgtt caggatgata tcaatatttt
     4861 aagagattta attgcaatta agtcaatttt tgctcagaaa gttggtttaa atgacttatc
     4921 aagctatctt ggagaagttt tcagaaaagc tggcgcagaa gtcattatag atgacagcta
     4981 tagcgctccc tttatcgtgg caaatttcaa atcatcaaaa gtagatgcta agcgtattat
     5041 tttttacaat cattatgata ctgtaccagc agatgaggta gaacagtgga ctgaggaccc
     5101 ttttacactt tcacttcgat atggtaagat gtatggacgt ggtgtagatg atgataaagg
     5161 tcacattacc gcacgtttat cagcagttaa aaaatattta tcaagacaca aaggggaact
     5221 tcctctagat atcactttta ttgtggaagg agcagaggaa tcagcttcag taggccttga
     5281 ttatta
//
LOCUS       DATRNK010000091         5196 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.091, whole genome shotgun sequence.
ACCESSION   DATRNK010000091 DATRNK010000000
VERSION     DATRNK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.091"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            148..567
                     /locus_tag="VBC52_001893"
     CDS             148..567
                     /locus_tag="VBC52_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEO6883452.1"
                     /translation="MDNCIFCKIISGEIPSSKVYEDDEVLAFLDITQTTTGHTLLIPK
                     KHVRNVLEMDEKTAQITFERLPKVARAVQAATKAKGMNIINNNEEIAGQTVFHAHVHL
                     VPRFDESDGIKIHYTTHEPDFEALAKLAKEIRKEIQL"
     gene            564..860
                     /locus_tag="VBC52_001894"
     CDS             564..860
                     /locus_tag="VBC52_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883453.1"
                     /translation="MKIGRLIALGLVSLGALELYKNRKTIKDSYQNTKNETDSAKLKL
                     ERIKNDLAIISQEKEKIRLISQELNHKFQVFNKDIQPRLEEINQRMAKYQEKDE"
     gene            complement(923..2230)
                     /locus_tag="VBC52_001895"
     CDS             complement(923..2230)
                     /locus_tag="VBC52_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6883454.1"
                     /translation="MKIWKKITLMFSAIILTTVIALGVYVASAYNFSTNELSKTFKDF
                     KLAKSKSHAIEETKPFSILLMGVDTGSEHRKSKWSGNSDSMILVTINPKTNKTTMTSL
                     ERDVLIKLSGPKNNGQTGVEAKLNAAYASGGAEMALMTVQDLLDINVDYFMQINMQGL
                     VDLVNAVGGITVTNKFDFPISIAANEPEYKAVVEPGTHKINGEQALVYSRMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALNSISSYKKILSAVSNNMQTNIEISSKTIPNLLAYKD
                     SLEHIKSYQLKGEDATLSDGGSYQILTKKHLLAVQNRIKKELDKKRSKTLKTSAILYE
                     DYYGTTASNDSSTYSSTQENNYNTTPYSEAPPSYSGNTTYSSETNQTTHQNYYNSSTP
                     ASNYSSNTNTGQADSSGSVNNHNGAATPNPNTGTQPVPGQTNP"
     gene            complement(2239..2799)
                     /locus_tag="VBC52_001896"
     CDS             complement(2239..2799)
                     /locus_tag="VBC52_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001890427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6883455.1"
                     /translation="MGIAQEKLLRRFKMSKQFAVDFIEASREEASAILEFLNTVTEET
                     DFILHTVSNQLSLSEMETFIENTLMTKNCICLIAKLKNKVIGLITIISQSDIEIEHVG
                     DLFIAVQKDYWGYGIGHILMEEAIEWASDNDITRRLELSVQGRNERAIHLYQKFGFEI
                     DGLQTRGIKRENGEFLDIYRMSKLID"
     gene            complement(2753..3262)
                     /gene="tsaE"
                     /locus_tag="VBC52_001897"
     CDS             complement(2753..3262)
                     /gene="tsaE"
                     /locus_tag="VBC52_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEO6883456.1"
                     /translation="MLWGCQNQQTVGRQRYFWYNISMFYTQNEEELIALGQKLGTVLK
                     SGDIVLLTGNLGAGKTTLTKGIAKGLDIKQMIKSPTYTIVREYEGRVPLYHLDVYRIG
                     DDPDSIDLDDFLFGQGVTVIEWGELLSDNLINNYLEIVITRSNQGRQVQLEAYGHRAR
                     EIIEAIQDV"
     gene            complement(3345..4766)
                     /locus_tag="VBC52_001898"
     CDS             complement(3345..4766)
                     /locus_tag="VBC52_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEO6883457.1"
                     /translation="MEKFFKLKEHGTTIRTEITAGLTTFFAMSYILFVNPAILSQTGM
                     PAQGVFLATIIGAVVATSVMAFYANLPYAQAPGMGLNAFFTYTVVFALGYTWQEALAM
                     VFICGLISLIITLTKVRKMIIESIPTTLKSAITAGIGTFLAYVGIKNAGFLKFSIDPG
                     TYDVVGKGAAKGLATITANSSATPGLVSFDNPAILLSLIGLSITIFFIVKGIRGGIIL
                     SILTTTLLGILMGVVKLDAINWEATNLSASFRDLKQVFGVALGEKGLISLFSNPSRLP
                     SVLMAILAFSLTDIFDTIGTLIGTGEKVGILATTGDNHESKSLDKALYSDLIGTTFGA
                     ICGTSNVTTYVESAAGIGAGGRTGLTALVVAGLFAISSFFSPLVSIVPSQATAPILVI
                     VGIMMLSNLKDIKWDDMSEAIPAFFTSLFMGFTYSITYGIAAGFLTYTLAKVIKGQAK
                     DIHVVLWILDILFILNFISLAIL"
     gene            complement(4869..>5196)
                     /locus_tag="VBC52_001899"
     CDS             complement(4869..>5196)
                     /locus_tag="VBC52_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEO6883458.1"
                     /translation="ILYVSSILFGFLSGIESFSVIMRKFRISNIFLRWGIIGIVSFVS
                     SFGIHIGRYARLNSWDILTKPQVVINELLAVPSRDSFHFILGFTFLQVLCLIFMDYSA
                     GKKLEK"
BASE COUNT         1764 a         1001 c          779 g         1652 t
ORIGIN      
        1 taagataagt atgatggtaa atatagatat cacaatcaat acgggctact ttcatttccc
       61 tgtacgactt atgacctatc aaacgataaa aatttaccat tccttaatag ctacccaata
      121 aatttttcaa catgaaagga attagttatg gataattgta ttttttgtaa aatcatcagt
      181 ggagaaatcc cttcatcaaa agtttatgaa gatgatgaag tattagcttt tctcgacatt
      241 acacaaacta cgaccgggca cacccttctt attcctaaaa aacatgttcg aaatgtgtta
      301 gagatggatg aaaaaactgc acagataacc tttgaacgct tgcctaaagt ggcgcgtgct
      361 gtccaagctg ctacaaaagc caaaggaatg aatattatta acaacaatga ggaaattgct
      421 ggacaaactg tttttcatgc acacgtccac cttgttcccc gctttgatga atcagacggt
      481 atcaaaattc actacactac tcatgaaccc gactttgagg cacttgcaaa attagccaaa
      541 gaaattagaa aagagattca attatgaaaa taggtcgttt aattgcacta ggtttagtta
      601 gtttaggagc gcttgaactt tacaaaaata gaaaaactat caaggattcc tatcaaaata
      661 caaaaaatga gacagattca gctaaattaa aattagaacg tatcaaaaat gatcttgcta
      721 ttattagtca ggaaaaagaa aaaatcagat taatttctca agaactcaat cataaattcc
      781 aagtgtttaa taaagatatt caacctcgtt tagaagaaat taatcaacgc atggctaaat
      841 atcaagagaa agacgaataa gccgacggta taaaaatatc tactctaaat ataaaaagag
      901 caaataatgc tctttttata ttttatggat tagtttgacc tggtactggt tgcgttcctg
      961 tgtttggatt aggcgttgca gccccgttat gattattgac acttccactt gaatcagcct
     1021 gacctgtgtt agtgttactg ctatagttac tagcaggagt gctactatta tagtaatttt
     1081 gatgagttgt ttgattagtc tcagaactat aagtagtatt accactgtaa cttggtggtg
     1141 cttctgaata aggtgttgta ttataattat tctcttgtgt tgatgaataa gtagaagaat
     1201 cattactagc agtagtacca tagtaatctt catatagaat cgcgcttgtc ttcagagttt
     1261 tactacgctt tttatccagt tctttcttaa ttctattttg aactgcaagt agatgtttct
     1321 tagttaaaat ttgataagag ccaccatctg ataaagtagc gtcttcaccc ttcaactgat
     1381 aagatttaat atgttccaat gaatctttat aagctaacaa attaggaatc gtttttgatg
     1441 atatctcaat attagtttgc atgttattac ttactgcgga aagaattttt ttgtatgaac
     1501 taatactatt taacgccaat atttttttaa ggactttttg aattacttca cgttgtcttt
     1561 tttgacgccc ataatctccc tctggatcat catagcgcat acgagaataa acaagtgctt
     1621 gttctccatt tattttatgt gtccctggtt caacaacagc cttgtactct ggttcattgg
     1681 cagcaattga tattggaaag tcaaatttat tagttactgt tataccacca acagcattga
     1741 ctaaatcaac taatccttgc atattaattt gcataaagta atcaacatta atatctaata
     1801 agtcttgaac agtcatcaat gccatttccg caccaccaga agcataggct gcatttagct
     1861 ttgcttctac tccagtctgt ccattatttt tgggaccact caatttaatc aatacgtcac
     1921 gttctaagct tgtcatcgtt gttttattag ttttaggatt tatagtgact aagatcatag
     1981 aatcgctatt tcctgaccac ttagattttc gatgctctga acctgtgtcc acccccatta
     2041 atagtattga aaacggcttt gtttcttcaa tagcatgact ttttgattta gctagcttaa
     2101 aatctttaaa agtcttagac aattcattag tcgaaaaatt ataagctgag gcaacataga
     2161 ctcccaatgc aattactgtt gttaaaataa ttgcagaaaa cattaaggtt atttttttcc
     2221 aaattttcat gtatattcct aatctatcaa cttactcatt ctgtaaatgt ctagaaattc
     2281 tccattttct cttttaatac ctcttgtttg aagcccatct atttcaaaac cgaatttttg
     2341 gtagaggtga atagcacgtt cattacgtcc ttggactgat agttctaagc ggcgtgtaat
     2401 atcgttatct gacgcccatt ctattgcttc ttccatgaga atatgaccaa taccatatcc
     2461 ccaataatcc ttctgaacgg caataaaaag atccccaacg tgttcaattt caatatcaga
     2521 ctgactaatg attgttatca agccgatcac tttatttttt aatttagcaa tcaaacaaat
     2581 gcaatttttc gtcattaagg tattttcaat aaacgtttcc atttctgaca aagatagttg
     2641 attgctcacc gtatgcaaaa taaaatctgt ttcctcagta acagtattta aaaattcaag
     2701 gatcgcagat gcttcctctc tcgaagcttc tataaaatca acagcaaatt gtttagacat
     2761 cttgaatcgc ctcaataatt tctcttgcgc gatgcccata agcttctaac tgaacctgac
     2821 gtccttgatt gctcctcgta ataacaattt ctaagtagtt attgataagg ttatcactaa
     2881 gcaactctcc ccattcaata actgttactc cttgaccaaa caagaagtca tctaagtcaa
     2941 tagaatcagg atcatctcca atacgataaa catccaaatg gtaaagtggt acacgccctt
     3001 catattctct aacaatagtg taagttgggc tcttaatcat ttgttttatg tctaatccct
     3061 ttgcaatacc ttttgtaaga gttgttttgc ctgcacctaa attaccagtc aataatacga
     3121 tatctcctga ctttaaaaca gtacccagtt tttgccctag ggcaataagc tcttcttcat
     3181 tttgagtata aaacatagag atattatacc agaaatatct ctgtcttcca acagtttgct
     3241 gattctgaca gccccatagc atatatttct tcacttttct taagaaaaaa ggaaaccagg
     3301 ctacaaatct gatttccttt tatcttattt atcaaactat aatattacaa aatagccaag
     3361 ctgataaagt ttagaataaa taatatatct aaaatccaca gtaccacatg aatatctttt
     3421 gcttgtcctt tgatgacttt agctagtgta taggtcaaga aaccagctgc aataccataa
     3481 gtaatgctat aagtaaaccc cataaaaaga gaagtaaaga aagctgggat agcttctgac
     3541 atatcatccc atttgatatc ttttaaattt gaaagcatca taataccaac aataaccaat
     3601 attggtgcag tagcttgact cggaacgata gatactagtg gactaaaaaa actagatata
     3661 gcaaacaaac cagcaactac taaggctgtc aatcctgtac gaccaccagc tccgattcct
     3721 gctgctgact caacataggt tgtaacgtta gatgttccac aaatagcacc aaaagttgtc
     3781 ccaattaagt ctgaatagag tgctttgtct aaactcttag attcatgatt atcaccagtc
     3841 gttgctaaga taccaacttt ttctccggta ccaatgagcg ttccaatggt atcaaaaata
     3901 tcagtcagtg aaaaagcaag aatggccatt aatacacttg gtagacgtga aggattagaa
     3961 aataaagaaa ttaatccttt ctctcctaat gcaactccaa atacttgctt caaatctcta
     4021 aaagaggccg agaggttagt tgcttcccaa ttaatagcat ctaacttaac cactcccatt
     4081 aaaataccta agagggtcgt tgttaatatc gatagaataa tccctccacg gatacccttt
     4141 acaataaaga agatggtaat gcttaaacca atcaaagata ataaaatggc tggattatca
     4201 aacgacacta gccctggcgt tgctgaagaa tttgctgtaa tagtagccaa tcctttcgca
     4261 gcaccctttc caacaacatc atacgtccca ggatcaattg aaaatttcaa aaaaccagca
     4321 tttttaatcc caacgtatgc taaaaaagtt ccgattcctg ctgtaatggc tgattttaaa
     4381 gttgttggga tagactcaat aatcatcttt ctaactttcg ttaaagtaat aatcagagaa
     4441 atcaaaccac aaataaatac catagctaag gcttcttgcc atgtatatcc caatgcaaag
     4501 acaactgtat atgtaaagaa agcatttaat cccatcccag gagcttgtgc atagggaagg
     4561 ttagcataga aagccataac agatgtagct acaacggcac caataatcgt cgctaagaaa
     4621 actccttgtg ctggcatccc agtctgagat aaaatagctg gatttacaaa caaaatgtac
     4681 gacattgcaa agaaagtcgt tagaccagca gtgatttcag tccgaattgt cgtcccgtgt
     4741 tcctttagct taaaaaactt ttccatttta aaatcggatt ctcctttatt ttttgctatt
     4801 ttacactttt aaaaaaatta atcgttctta tatttacgaa cgactaatat atattaacaa
     4861 aaaataattc acttttcaag ctttttccct gcagaataat ccataaaaat aagacacaat
     4921 acctgtaaaa atgtaaatcc taaaataaag tggaatgaat ccctactcgg aaccgctaaa
     4981 agttcgttaa ttaccacttg tggcttcgtt aagatatccc atgaatttag acgggcataa
     5041 cgaccaatat gaataccaaa acttgaaaca aatgatacaa ttccaataat tccccatctt
     5101 aaaaatatat tgctaattct aaatttacgc ataatcacag aaaaactttc gatacctgat
     5161 aaaaaaccaa acaagatact agatacatac aagata
//
LOCUS       DATRNK010000092         5171 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.092, whole genome shotgun sequence.
ACCESSION   DATRNK010000092 DATRNK010000000
VERSION     DATRNK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.092"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            238..2139
                     /locus_tag="VBC52_001900"
     CDS             238..2139
                     /locus_tag="VBC52_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEO6883459.1"
                     /translation="METQILNSLHSVLKQFGNKYYTGSYLNKSKVIEDIDKYDEELIL
                     AMLNAPLIKKHYFKKIDEYTIFETNKLIETFEMNDYWMDSLTKYTKRIGLTSNGKFLD
                     ESSDIVLDFPYKDTVLKAGMTKEDIEKDDLKPDEPFYNEVIASEEIDIMLDKKILINA
                     KRYDVEGEHEITNFDDKDNLIIKGNNLLALHTLKDKYAGKVKLIYIDPPYNTGNDSFM
                     YNDRFNHSTWLNFIYNRLSIANELLSEVGSIWLNIDDDESHYLKVLCDELFGRENFIG
                     NIIWEKKFSPQNDATFFSDMHDHILVYCKNIDKFKINLLARTEKMNERYKNPDNDPRG
                     PWSSSDLTVRTYSKEYDYPIETPSGKIINPPKGRCWRTSKENLSKLISQNRIWFGESG
                     DNVPRLKRFLTDVKQGLTPGTIWKHQEVSHNQEARKEISRLFEDTEYDFSTPKPEKLL
                     QRIIHIGSNEGDLVLDFFMGSATTQAVAMKMNRRFIGIEQMDYINTVSVPRLQKVIEG
                     EQGGISKAVDWHGGGSFIYTELMEKSQGYINQIYKAIDSESLFKIYNLMLKNVDINFK
                     VDLQELEDILEKEPLLDEQKKLLLKVIDKNQLYYNYSEIDDVNVRDLISDTDYSFNQS
                     FYKEDDFIG"
     gene            2123..4831
                     /locus_tag="VBC52_001901"
     CDS             2123..4831
                     /locus_tag="VBC52_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEO6883460.1"
                     /translation="MISLVKKSSLPLLDALMAYDGTLEERTINYQTPQYIYDNMKHHF
                     RYYQDKALHYFHFSLNYNEFKYLQSNHVLFNMATGSGKTDLMAGIILYLFKEKGYQRF
                     LFTVNTNSVLQKTIDNLTDISSIKNLFNHPIEIESNRIRIEQVKQFPKISKENVIYIK
                     LDTIQGIASDLFTEKENTMGLTEYKENKTVILADEAHHYSASTKKEKDDERSWERTIE
                     LILESNVENRLLEFTATIDLENKHVYEKYKDKIIFRYSLDKYILDGYSKNIRRIQSSN
                     KDIDNMMDVILLSEYRRLYAKQMYNVEIKPVILFKSPKIIDSNQAESKFLNLIENLNP
                     QQIESFIRLRFNEKSEEQSETLYRTYSYYLNNVNNLPEIVREMKRQFSNNRIINANDT
                     DRGNMLEKGYYEVLNTLENPSNLYRVVFAVAKLTEGWDVLNLYDIVRISDTDNAKGNK
                     VSTMAEAQLIGRGARYNPFRLNGEISHKRRFEAGGADSLLLETLHYHTINEPQYLKNL
                     VESLNEMNLPTGEDKKNPLIDVKVKTSFKKTDIWKSGLIFYNERVIVSDDHYDNLSKY
                     GVDNRSDIIVNWQFNTKEYNYDSNKVQDDFSDTFQKVIQLDSRLVMKAFNRNSFYHFD
                     HLKKYIPVLKSRKEFLNESWLNLNNRTIYAVIPKSMGSKDLSPESKLEIFEIYLRQVE
                     QQIRHGYRKEIGTNKFIGYPIREYISDYKKRQPNYDTGRMNLFGMKVFQYVSRQDYIN
                     EDFFVYDSAFINQTEQQLVARIKERIAVLTEKYSEVYLVRMDENMHRESVKSDKLKLH
                     QFDRDTNDIHFEAFQPDFILLLANSDYYLQIFIEPKGIKLLESEQWKEDLLMYINDHE
                     SDLVFEEEVDGVKIKGLKFYTINDGRGAIEQLGEIALGEKFSGLSL"
BASE COUNT         2025 a          615 c          911 g         1620 t
ORIGIN      
        1 taataatggt tcatcaatta tattatgatt tgtttgcatg gaaaatagaa aaaataaaat
       61 taccaagtaa tcataaagta atagaattat agtcgaaaca tgatgtatat aaagaaagta
      121 ttgattgaag aagaactatt atttgacaaa tgcgagtggg gaataaatgt caagtgaatt
      181 attatctggt aagcatagac aatactataa attgaaacat aaaataggag tgaatgtatg
      241 gaaacgcaaa ttctgaatag tttacattct gtattgaaac aatttggtaa taaatattat
      301 acaggaagtt atttaaataa atctaaagta attgaggata ttgacaaata tgatgaagaa
      361 ttaattttag caatgttgaa tgcgccttta attaaaaagc attactttaa aaaaatcgat
      421 gaatacacga tatttgaaac caataaattg attgagacat tcgaaatgaa tgactattgg
      481 atggattcac ttacaaaata tactaagaga ataggattaa cttctaatgg gaagttttta
      541 gatgaatcat ccgatatagt gcttgatttt ccatataagg acacagttct aaaggcagga
      601 atgacaaagg aagatattga aaaagatgat ttgaagcctg atgagccttt ttataacgag
      661 gtaattgctt ccgaagaaat tgacatcatg ttagataaaa aaattttaat taatgcaaaa
      721 aggtatgatg tagagggaga acatgaaatt actaattttg atgataagga taatttaatc
      781 attaaaggaa ataatttatt agcattacat actttgaagg ataagtatgc tggaaaagta
      841 aagttgattt atattgatcc accatataac actggaaatg attcatttat gtataatgat
      901 agatttaacc attcaacatg gttgaatttt atttataata gattatctat agctaatgag
      961 ttattaagtg aagtgggatc aatttggtta aatattgatg atgatgagtc acactattta
     1021 aaagtcctct gcgatgaatt atttggtaga gagaacttta taggaaatat catatgggaa
     1081 aagaaatttt cccctcaaaa tgatgcgact ttcttttctg atatgcacga tcatatactt
     1141 gtatattgta aaaatataga taaattcaaa ataaacttgt tggcgagaac tgaaaaaatg
     1201 aatgaaagat ataaaaatcc ggataatgat ccgagaggtc catggtcatc ttcagattta
     1261 acagttcgga cttattcaaa agaatacgat tatcctattg aaacaccgtc aggaaaaata
     1321 ataaatccac ccaaaggtag atgttggaga acttcaaaag aaaatttaag taaattaata
     1381 tcacaaaata gaatttggtt tggagaatca ggagataacg ttcctaggct aaagagattt
     1441 ttaactgatg taaaacaagg actaactcct ggaactattt ggaaacatca agaagtttca
     1501 cacaatcaag aagctagaaa agagatttct agattatttg aagatactga gtatgatttt
     1561 tccacaccta aaccagaaaa gttacttcaa agaattattc atataggctc caatgaagga
     1621 gatttagttc tcgacttttt catgggttca gcaaccaccc aagctgtcgc tatgaagatg
     1681 aatcgtcgat ttattggaat tgaacaaatg gactatatta ataccgtatc tgtaccccgc
     1741 cttcaaaaag ttattgaagg agagcaaggg ggtatttcta aagctgttga ttggcatggg
     1801 ggcggttcat ttatttatac cgaattaatg gagaagagtc aaggttatat aaatcaaata
     1861 tataaagcaa ttgatagcga aagcttattc aagatttata atctaatgct aaagaatgta
     1921 gatataaatt tcaaagtaga tttacaagaa ttagaggata ttctagagaa agaaccatta
     1981 ttagatgaac agaaaaaatt gttattgaaa gtaattgata agaatcaact ctattataat
     2041 tattctgaaa ttgatgatgt gaatgtccgt gatttaattt cagatactga ttactcattt
     2101 aatcagtcat tctataagga ggatgatttc attggttaag aaatcaagtt tacctctatt
     2161 agatgcattg atggcatatg atggaacttt agaagaacga acgataaatt atcaaacccc
     2221 acagtatata tatgataata tgaaacatca ttttagatat tatcaagata aggcactaca
     2281 ttattttcac ttttcattaa attataatga gtttaaatac ttgcaatcaa atcatgttct
     2341 attcaacatg gcaacaggat ctgggaaaac tgatctaatg gcaggtataa tcttatattt
     2401 gtttaaagag aaggggtatc aaagatttct atttacggta aatacaaact cagtcctaca
     2461 aaaaaccatt gataatttaa cggatattag ctccataaaa aatttattca accatcccat
     2521 tgaaattgaa agcaatcgaa ttcgtattga acaagtaaaa cagtttccaa agatatctaa
     2581 agaaaatgtt atttacataa agttagatac aattcaaggt attgcaagtg atttatttac
     2641 tgaaaaagaa aatactatgg gattgacaga atataaggaa aataaaacgg ttattttagc
     2701 ggatgaagcc catcattatt cagctagtac aaagaaagaa aaagatgatg aacgttcttg
     2761 ggaaagaacg attgagttga tactggaatc aaatgttgag aatcgattat tagagtttac
     2821 ggcaactatt gatttagaga acaaacacgt ctatgagaaa tataaagata aaataatatt
     2881 tcgttattcg ctggacaagt atatacttga tggatattca aagaacattc gaaggattca
     2941 atccagcaac aaagatatag ataatatgat ggatgttatt ttgttaagcg agtatagaag
     3001 gttgtatgct aaacaaatgt ataatgttga aattaagccg gttatattat ttaaatctcc
     3061 gaaaataatt gattctaatc aagccgaaag taaattttta aatctaattg aaaacctaaa
     3121 tccacaacaa atagagtcgt ttattagatt gagatttaat gaaaagtcag aagaacagag
     3181 tgaaactctg tatagaacat atagttacta tttgaacaac gttaataact tacctgaaat
     3241 tgttagagaa atgaaacgtc aattttctaa taaccgaatc attaatgcca atgatactga
     3301 tcgtgggaac atgttagaga aaggttatta tgaagtatta aatacattag aaaatccttc
     3361 aaacttatac agagtcgttt ttgctgtcgc gaaattaact gaaggatggg atgtgctaaa
     3421 cctttatgat attgtacgta tttctgatac tgataatgct aaggggaata aagtatcaac
     3481 catggcagaa gcacaactta tagggcgtgg agctagatat aatcctttta gattgaatgg
     3541 tgaaatttca cataaaagac gtttcgaagc tggcggtgct gatagtttat tacttgaaac
     3601 attgcactac catactatta atgaaccaca atatctaaaa aatctggtgg agtcattgaa
     3661 tgaaatgaat ttaccaactg gggaagataa gaaaaatcct ttgattgacg ttaaagtaaa
     3721 gacttcattc aagaaaacag atatttggaa gagtggattg atattttata atgaacgggt
     3781 tatagtatct gatgatcatt atgacaattt atcaaaatat ggtgtagaca atcgttcaga
     3841 tattattgtt aattggcaat ttaatacaaa ggaatataat tacgactcta ataaggttca
     3901 agatgatttt tctgatacat tccaaaaagt aattcaatta gattcaagat tagttatgaa
     3961 agcctttaat cgaaattcat tttatcattt tgatcattta aaaaagtata taccagtatt
     4021 aaaatcgaga aaagaattct tgaatgaatc atggttaaac ttaaataata gaaccatcta
     4081 tgctgtaata cctaaaagta tgggaagcaa agaccttagt ccagaatcga aactagagat
     4141 atttgagatt tatttaaggc aggtggagca acaaatcagg catggttatc gtaaagaaat
     4201 tggaaccaat aaatttatag gatatccaat acgagaatat ataagtgatt ataagaaacg
     4261 tcaaccgaat tatgatactg gacggatgaa tttatttggt atgaaagttt ttcagtatgt
     4321 aagtagacaa gattatatta atgaagattt cttcgtatat gattcagcat ttataaatca
     4381 aacagagcaa caattggtag ctcgaatcaa agaacgaatt gcagtactaa ctgaaaagta
     4441 tagtgaagta tatttagtac gtatggatga aaatatgcat cgtgagtctg taaaaagcga
     4501 caaattgaag ttgcatcaat ttgatagaga taccaacgat attcatttcg aagcatttca
     4561 acctgatttt atcttattac ttgccaactc agattattat ttacagatat ttatcgaacc
     4621 aaaaggtatc aaacttttag agagtgagca gtggaaagaa gatttattaa tgtatattaa
     4681 cgatcatgaa tccgatttag tgttcgagga agaagtagat ggcgtgaaga ttaaaggatt
     4741 gaagttctat actatcaatg atggacgagg agctattgag caattaggtg agatagcatt
     4801 aggtgaaaag tttagtggtt tgagtcttta aatttaatgt gaaagtcaaa ttgattagaa
     4861 gaaaagaaat atttacgaga atattatcac ctaccacttc ttttaagtgg actttaaaga
     4921 gtcaagctaa aatacttgaa ttaaggggag ttatacagat tctttcagat aaagtttcaa
     4981 aatcaataat aattgaattt aaatagtagt gtgaacattt cggtaaattg ctagctctat
     5041 ttaaataact tagtgaaata ttcacaccat tcaactgaat ctagttgaaa cttattaaaa
     5101 tttatattaa ctaaaattct aaaaaacatc ggtgttaagg agtttggcaa tttattcaaa
     5161 ctaatacttt t
//
LOCUS       DATRNK010000093         5086 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.093, whole genome shotgun sequence.
ACCESSION   DATRNK010000093 DATRNK010000000
VERSION     DATRNK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.093"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            13..897
                     /locus_tag="VBC52_001902"
     CDS             13..897
                     /locus_tag="VBC52_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6883461.1"
                     /translation="MDIRQLTYFIAVAEAKNYSHAAKSLFVTQPTLSQSIKKLEAELN
                     TILFLQNGRQLALTEAGEILYEKGQLLMTNFNQMVTEIQQLNQEKKEGIRVGLTSLFA
                     IQFMKQISTFMATHSNVEVSLIQDGSRKLQELLAKGKIDIGLLSFPSTRNDITIEPLQ
                     TSTKGYKVSIVMPKSHPLATLPEIELNDLRDYKVASLNEHYMLGEMLPRKCRALGFDP
                     HIVFKHNDWEVLIHSLQDLNAVTILPSEFESISQVQDLCWVPLKDKNNFYPIGIAYRN
                     DTSFSPMIEEFLSLLKTN"
     gene            complement(934..1752)
                     /locus_tag="VBC52_001903"
     CDS             complement(934..1752)
                     /locus_tag="VBC52_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEO6883462.1"
                     /translation="MILKICRAAYSLQWGGVYQLALLDYPRIKAFELERIGAFIAYEK
                     QYKRKIEIQCDDKHLLTKIVHFLKYNSFTFPYIPKYREAAATFNEDGISLTSDFLSHT
                     CTIETAKLIFKEGKILSAVKAFNKPAEVLVNDKRNAAGDPKDYFDYVMLNWSNTNSGY
                     RLVMERLLGKAPSEQELTVAFKPGVSFHFNYQDIINHPDSIFDGYHPAKIKNQLSLAE
                     HLVACVIPKHYQEDYQSLVPNDLKHRVYYLDYCNETLYEWNQKVYDFLCHLENK"
     gene            complement(1916..2689)
                     /gene="fetB"
                     /locus_tag="VBC52_001904"
     CDS             complement(1916..2689)
                     /gene="fetB"
                     /locus_tag="VBC52_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEO6883463.1"
                     /translation="MSGANSISLMSLLMASSLVLITLFFSYWQKLNLEKEVIISAIRA
                     VIQLLAVGFLLDYIFGYQNPIFTALLMLFMIINASYNAAKRGKGINKGFVISFIAIGS
                     GTIITLSVLIFSGILKFVPNQMIPVGGMIISNSMVAIGLCYKQLLSEFRSKQEEVETK
                     LALGADILPASIDIIRDVIKTGMVPTIDSAKTLGIVSLPGMMTGLILAGTSPIQAVKY
                     QMMVTFMLLATTSIASFVATYLAYKIFFNNRKQLVVTKD"
     gene            complement(2686..3342)
                     /locus_tag="VBC52_001905"
     CDS             complement(2686..3342)
                     /locus_tag="VBC52_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6883464.1"
                     /translation="MSYLTFKHVDFKTDDKLVLNDINFAIDEGDFVSIVGPSGSGKST
                     VLKLASGLMSPTAGHIFFDGKDLNQLEPIESRKMISYCFQTPHLFGNTVEDNISFPYH
                     IRHEKVDYRRVDDLFQRFEMDQSYLKQDVKKLSGGEKQRIALIRQLLFEPKVLLLDEV
                     TSALDNHNKVIVEKVIKSLHDKGITILWITHDEEQSRRFANKVLKVVNGSIEAMEVIK
                     "
     gene            3559..4197
                     /locus_tag="VBC52_001906"
     CDS             3559..4197
                     /locus_tag="VBC52_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEO6883465.1"
                     /translation="MFLIDFILHIDTHIYAMANTVGNWTYLLLFLVIFVETGAVIFPF
                     LPGDSLLFAAGALAANPKMSFNIVTFLIIFFIAAFIGDSCNFLIGRTFGYRFIKHPFF
                     RRFIKEKNIRDAELYFEKKGTAAIILGRYIPIIRTFVPFVAGISQLPPKVFIKRAFIA
                     ALSWSVIATGSGFLFGNIPFVKQHFSLIILGIVFVTLIPVLISGVKSYRNTK"
     gene            complement(4275..5060)
                     /locus_tag="VBC52_001907"
     CDS             complement(4275..5060)
                     /locus_tag="VBC52_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6883466.1"
                     /translation="MKRLHKLFITVIATLGMLGVMTFGLPTQPQNVTPIVHADVNSSV
                     DTSQEFQNNLKNAIGNLPFQYVNGIYELNNNQTNLNADVNVKAYVQNTIDNQQRPSTA
                     NAMLDRTIRQYQNRRDTTLPDANWKPLGWHQVATNDHYGHAVDKGHLIAYALAGNFKG
                     WDASVSNPQNVVTQTAHSNQSNQKINRGQNYYESLVRKAVDQNKRVRYRVTPLYRNDT
                     DLVPFAMHLEAKSQDGTLEFNVAIPNTQASYTMDYATGEITLN"
BASE COUNT         1660 a          917 c          760 g         1749 t
ORIGIN      
        1 aggacagata ttatggatat tagacaactt acttacttta ttgctgttgc agaagcaaaa
       61 aattattcac acgctgcaaa aagtcttttt gtgacccaac ctacactttc acaatccatt
      121 aaaaaattag aagcagaatt aaatactatt ttattcttac aaaatggtcg tcaactcgct
      181 ttaactgaag caggagaaat tctctatgag aaaggacaat tgttgatgac taattttaat
      241 caaatggtta cggaaatcca gcaattaaat caagaaaaaa aggaaggtat ccgtgtaggg
      301 cttacttctc tttttgctat tcaatttatg aaacagattt caacttttat ggctacacat
      361 tcaaatgttg aagtatcttt gattcaagat ggttcccgta aattacaaga attgttagca
      421 aaaggaaaga tcgatattgg cttattgtcc tttccaagta cccgcaatga tatcactatc
      481 gaaccactgc aaacctcaac taaaggatat aaagtaagca ttgtcatgcc aaaatcgcat
      541 cctcttgcaa cacttcctga aattgaactt aatgatttaa gagattacaa agttgcctca
      601 ctcaacgaac attacatgct aggagagatg ttgcctcgta aatgtcgtgc tttaggattt
      661 gacccccata tcgtctttaa acacaatgac tgggaagttc ttattcatag tttacaagac
      721 ctcaatgcag tcactatttt accaagtgaa tttgaaagca ttagccaagt tcaagattta
      781 tgctgggtcc cacttaagga taaaaataat ttttatccga ttggtattgc ttaccgaaat
      841 gatacttctt ttagtcctat gattgaagaa tttttaagtc ttttaaagac caattaatag
      901 cagttaaacc aaaaagttta aaactttttg gttttattta ttttccaaat gacaaagaaa
      961 atcataaact ttttgattcc actcataaag tgtttcgtta cagtaatcta aataataaac
     1021 tctgtgtttc aagtcattag gcacaaggct ttgataatct tcttgataat gttttgggat
     1081 aacacatgca actaaatgtt ctgctaaaga aagttgattt ttaattttag caggatgata
     1141 accatcaaaa atagaatcag gatgattgat gatatcttga taattaaaat gaaagctgac
     1201 ccctggctta aaagctactg ttaactcctg ttcagatggt gctttgccta acaatctttc
     1261 cattactaaa cgataaccag aattggtatt tgaccagttc aacatcacat agtcaaagta
     1321 atctttaggg tctccagcag cattcctctt atcatttacc agtacttcag caggcttatt
     1381 aaaggcttta actgctgata agattttacc ttctttaaaa attagttttg cagtttcaat
     1441 cgtacatgta tggcttaaaa aatcagaagt taaactaata ccatcctcat taaaagtagc
     1501 tgccgcttct ctatatttgg gaatataggg aaaagtaaaa ctattgtatt ttaaaaaatg
     1561 aacaattttt gtgaggagat gtttatcgtc acattgtatc tcaatttttc ttttatattg
     1621 tttctcgtaa gctatgaaag ctcctatcct ttccaattca aacgccttaa ttcgaggata
     1681 atccagcaaa gctaattggt aaacacctcc ccattgtaaa ctatatgctg cacgacaaat
     1741 ttttaaaatc atttcttcct ccttttgata atccctataa caatttctat taaatagtag
     1801 catttaaacg taatcgactc aataacttag tcaattttcg atcaaaaata agattaagat
     1861 accaaaaaag cctggactat acttaccaga ctaacatact gtttattaaa atgaatcaat
     1921 cttttgttac tactaattgc ttacgattat taaagaaaat tttataagcc aaataagtag
     1981 ctacaaaaga agctatcgaa gtcgttgcta atagcataaa cgtcaccatc atttggtatt
     2041 taaccgcttg gataggtgaa gttccagcaa gaattaaccc tgtcatcatc cctggcaaag
     2101 atacaatccc aagtgtttta gctgaatcaa ttgtaggtac cataccggtt ttgataacat
     2161 cacgaataat atcaatcgaa gctggcaaaa tatctgctcc taaagctaat tttgtctcca
     2221 cttcctcttg tttacttcta aattcggaca ataattgttt atagcacaaa ccgattgcta
     2281 ccatagaatt actaataatc ataccaccta caggaatcat ttgattcgga acaaatttta
     2341 aaattcctga aaaaatcaag acagataaag tgattatagt ccctgaccca atagctataa
     2401 atgaaataac aaacccttta ttaatccctt taccacgctt agctgcgtta taagaagcat
     2461 taataatcat gaatagcatt aaaagagctg tgaaaatagg attttgataa ccaaatatat
     2521 agtctagcaa aaaccccact gctaataatt gaatcactgc tcggatagcg ctaataatca
     2581 cttctttttc taggtttaac ttttgccagt aagaaaaaaa taaagtaatc aaaactaatg
     2641 atgatgccat tagtaatgac attaaagaaa tagaatttgc accgctcatt ttataacctc
     2701 cattgcttca atactaccat tgacaacttt taagacttta ttggcaaatc gacgactctg
     2761 ctcctcatca tgcgtaatcc acaaaatcgt tattcctttg tcatgtaatg atttaataac
     2821 cttttcaaca ataactttat tatgattgtc taaagccgaa gtaacttcat ctaagagtaa
     2881 aactttaggt tcaaacaata attgcctaat cagagcaata cgttgctttt ctcctcctga
     2941 tagttttttg acatcctgtt tcaggtaaga ctggtccatt tcaaaacgtt gaaataaatc
     3001 atcaacgcgt ctataatcta ctttttcgtg tcgaatgtgg taaggaaaac taatattgtc
     3061 ttccactgta ttaccaaata aatgtggcgt ttgaaaacag taagaaatca tttttcgact
     3121 ttcaatcggt tctaattggt tcaaatcttt tccatcaaag aaaatatgac ctgcagtagg
     3181 actcattaaa ccactggcca attttaaaac agtactctta ccacttccag atggtcctac
     3241 aatagaaaca aaatcacctt catcaattgc aaagttgata tcatttaaaa ccaacttatc
     3301 atccgtttta aaatcaacgt gtttaaaggt taaataggac attgttaagc tcctttactt
     3361 cttatttata atcattataa ttaagtctaa cataaaaatc aaaaaaaagc tctttagaat
     3421 aattctaaat taataagtaa acttatttta cctcttatta aaaattttct gataaaatag
     3481 gctttgtaag aggccttata aacaagatag ttatcatcca agtctctgca ctttagttga
     3541 tcaataaagg agactcctat gtttttaatc gattttattt tacatattga tactcatatc
     3601 tatgctatgg ctaacactgt tggtaattgg acctatctat tgctcttcct cgttattttc
     3661 gttgaaactg gggctgtcat ctttccattt ttaccaggtg atagtttatt atttgctgct
     3721 ggagcccttg ctgcaaatcc aaaaatgtcc ttcaatattg taactttcct tattattttc
     3781 tttattgcag catttattgg tgatagttgc aatttcttga ttggtcgtac atttggttac
     3841 cgttttatca aacatccttt ctttcgtcgt ttcattaaag aaaaaaatat tcgagatgct
     3901 gagctttatt ttgagaaaaa aggtactgct gccatcattt tagggcgcta tatccctatt
     3961 atcagaacct ttgttccttt tgtagcaggt attagtcagc tccctccaaa agtatttatc
     4021 aaacgcgcct tcattgcagc attatcttgg tcagttattg caacaggttc tggttttctc
     4081 tttggtaata ttccttttgt gaagcagcac ttctcgctta ttatcttggg aattgtattt
     4141 gttaccttaa ttcctgtcct tatttctggt gtcaaatcat atcggaacac taagtaatgt
     4201 atccaaaaat ttctatttaa cactattaat cactaagaat gacaacaaaa aaagagcaat
     4261 tagttgctct tttattaatt tagtgttatt tctcctgttg cataatccat agtgtatgat
     4321 gcttgtgtgt ttggaatagc aacattaaat tctaatgtgc catcttgtga tttagcttct
     4381 aggtgcattg caaatggaac taaatcagta tcattacggt acaatggagt tacacggtaa
     4441 cgaacacgtt tgttttggtc aaccgcctta cgaactaagc tttcataata attttgtcca
     4501 cgattgattt tttgatttga ttggttggaa tgagctgttt gtgtgacaac attttgagga
     4561 tttgacacgg aagcatccca acctttgaaa tttccagcta aagcataggc aattaaatgc
     4621 cccttgtcga ctgcatgccc ataatggtca ttagtagcta cttgatgcca acctaatggt
     4681 ttccaatttg catcgggaag agtggtatct ctgcgatttt gatattgacg aatggttcta
     4741 tcaagcattg cattagcagt tgatggtctt tgttgattgt caattgtatt ttgaacatac
     4801 gctttaacat tgacatcagc atttaaattt gtttgattat tatttaattc ataaatacca
     4861 ttaacatatt gaaatggtag gttaccaata gcatttttta aattattttg aaattcctgg
     4921 ctcgtatcaa cagatgaatt gacatcagca tgtactatcg gtgttacgtt ttgcggctgc
     4981 gttggaagac caaaggtcat tacccccaac atacctaatg tagcaattac ggttataaac
     5041 agtttatgta atcttttcat aagctttacc tctttctaat tacatt
//
LOCUS       DATRNK010000094         4957 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.094, whole genome shotgun sequence.
ACCESSION   DATRNK010000094 DATRNK010000000
VERSION     DATRNK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.094"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..454)
                     /locus_tag="VBC52_001908"
     CDS             complement(<1..454)
                     /locus_tag="VBC52_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEO6883467.1"
                     /translation="MRITIFCGASTGENPVYSEKTVALAQWMAQNKHSLVYGGGKVGL
                     MGVMADTVIANGGYTTGVIPTFLRDREIAHENLSELIIVNNMPERKAKMMLLGDAFIA
                     LPGGPGTLEEISEVISWSRIGQNDNPCILYNVNGYFNDLKNMFDHMVGE"
     gene            complement(451..768)
                     /locus_tag="VBC52_001909"
     CDS             complement(451..768)
                     /locus_tag="VBC52_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEO6883468.1"
                     /translation="MDITDYQKWVSEFYKKRNWYQYNSFIRSNFLSEEVGELAQAIRK
                     YEIGRDRPDETEQTDLENLNDIKEELGDVLDNIFILADQYNISLEEIISAHRTKLEKR
                     FET"
     gene            982..1596
                     /locus_tag="VBC52_001910"
     CDS             982..1596
                     /locus_tag="VBC52_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6883469.1"
                     /translation="MNKKPFFQCVILFVSFLVLTLAVKSQSDMISYLDNITSAFFQSI
                     RNPDLTNLMTIISTVVSPLTTSLIALVILGYQYFLNERIAVWLFMLFFGTNALALLLK
                     DIIARHRPMNQLVFDSGYSFPSGHTISTFLLMILVLVVARQRLRRVLSQVVFVIFALV
                     ILASVIFSRLYLENHFLTDILGSLLLGATSYCGLSAIVSLKELQ"
     gene            1845..2921
                     /locus_tag="VBC52_001911"
     CDS             1845..2921
                     /locus_tag="VBC52_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6883470.1"
                     /translation="MLGENIYLQRTQIGMTQENLSDYLHLTKTTISKWENNQAKPDID
                     YLILMANLFDISLDDLVGYQKTLSDDQRNQLIKDLKIKANVLSERDFFQEVKELSKQF
                     PNDFKTLLIMINMVLSNLTNLNDSEMKEWSLSTLNKIISKTVVESDLQTAIMFKTVVL
                     FHNLEYDEVINLYQNSPYKLGEELLLANSFFAKGDVYRAKQVLQVEIYQQILLVCEYL
                     LSLSTFEVEENKGDIFERLEHMIEAFQLNSLHPNTVIKCYYSLACHYSKSDDDKALGY
                     LKQCFKSLKQLLQRFELHGDTFFYTIDDWLETIPTGIAPPTSQLQVIERVEDLFQNSQ
                     FKELSGRKEFQQMIQKLNDLKKDY"
     gene            2923..3129
                     /locus_tag="VBC52_001912"
     CDS             2923..3129
                     /locus_tag="VBC52_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEO6883471.1"
                     /translation="MNTEIPNQLLPFLVPLIMLQGALIIISLVKLSKLKMTKHLSKPV
                     WFLVIIFLNIIGPIAFLILEGNNE"
     gene            3126..4028
                     /locus_tag="VBC52_001913"
     CDS             3126..4028
                     /locus_tag="VBC52_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6883472.1"
                     /translation="MKAISFQNVTKSFGPKKILNNVSFDLEENMIYGFVGPNGAGKTT
                     TIKMILGLLKFDTGFITIFGKKVNFGRTDTNQLIGYLPDVPEYYDYMTALEYLDLCSG
                     LARSKHKLSNKELLRSVGLDDNHQKIATYSRGMKQRLGLAQALVHDPKIIICDEPTSA
                     LDPKGRQDILDIISNLRGEKTVIFSTHILSDVEKICDHVLVLTKCGIYSLEELKGKKS
                     EENYSVRILIKVTKSEAKVLSHNYQIEKKDNEYALTLKGSKMDNKADLLAGFYQDLVS
                     LKISPSAIEVIDNSLEELYLEVTK"
     gene            4025..4786
                     /locus_tag="VBC52_001914"
     CDS             4025..4786
                     /locus_tag="VBC52_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6883473.1"
                     /translation="MKSLAVMLKKEWMENVRTYKVISILITCSIFGILGPLTALMMPD
                     IMAGILPKKLQGAIPEPTYIDSYIQYFKNMNQLGLVILVFLFSSTLTQEFSKGTLINL
                     VTKGLAKKVIILAKFIVITLLWTVSYLLSVVIHFSYTLYYFNNEGSHKLMVYGATWFI
                     GILFISLILFFSVLFRKTLGVLLGCLFMVVLCVVSGFFKISKSYNPMLLIQKQGEMLS
                     GKFKVQDLMEPSILVFLLIIMTLLLSMYIFEKSEV"
BASE COUNT         1646 a          745 c          824 g         1742 t
ORIGIN      
        1 cctcaccaac catatgatca aacatatttt ttaaatcatt aaagtagcca ttaacattat
       61 ataaaatgca aggattatca ttttgaccga tgcgagacca agagataact tcggaaattt
      121 cttcaagggt accaggtcca ccaggtagag cgataaaggc atctccaagt agcatcattt
      181 ttgccttacg ttcaggcata ttatttacta tgataagctc tgataaattt tcatgtgcaa
      241 tttcacgatc tcttaaaaat gtcggaataa caccagtagt atagccacca tttgcaatca
      301 cagtatcagc catgactccc attaagccaa cttttcctcc accatacacc agagaatgtt
      361 tgttttgtgc catccattgt gctaaggcaa ctgttttttc actataaaca ggattttccc
      421 cagtacttgc tccacaaaaa atagttattc tcatgtttca aacctctttt ctaatttagt
      481 gcgatgtgcg ctgataatct cttctagtga gatattatac tgatcagcaa gtatgaagat
      541 attatctaag acatctccta gttcttcttt tatatcattt aagttttcca aatcagtttg
      601 ttcggtttcg tcaggtctgt cacgtccaat ttcatatttg cgaatggctt gagccaattc
      661 gccgacttct tctgaaagaa agttacttct aataaaagaa ttgtattgat accagtttct
      721 ttttttatag aattcgctca cccatttttg ataatctgta atatccattg ttccctccgt
      781 ttcaagtata ttgtagcaat attgctttat taatgaaaat ttgaacgcta attaatagga
      841 aaagttcgta tttggtattt cttctagttt gtgaatagtc atatttaaac ctaagaggtt
      901 agcgcgtgct ttaagcattt tttaaatatt ttaaaccata atgtatgaag atttatctct
      961 ttattaaagt gaggttatgt agtgaacaag aaacccttct tccagtgtgt tatactattt
     1021 gtttcttttt tagtgttaac attagcagtt aaatctcaaa gtgatatgat aagttatttg
     1081 gataatatca cttcagcttt ttttcaaagc atacgaaacc ccgatttaac taacttaatg
     1141 acgataatat caacagttgt cagccctttg acaacgtcat taatagcact tgttatcctt
     1201 gggtatcaat attttttaaa cgaaaggata gctgtgtggc tatttatgtt attttttggt
     1261 accaatgctt tggcactgct tttaaaagat atcatcgcac gtcatagacc tatgaatcaa
     1321 cttgtttttg attcgggtta tagttttccc agtggacata cgatatcaac gtttttatta
     1381 atgattcttg ttttagtggt tgcaagacag agattgagaa gagtattgtc acaagtagtt
     1441 ttcgtaatat ttgcattagt gatccttgca tcagttattt tttcaagact ctatttagaa
     1501 aatcattttt taacggatat tttagggtcg ttgttactag gagcaacgtc atattgtggt
     1561 ttgtctgcta ttgtatcttt gaaagagtta caataaaaat agtagttacc aatgaattat
     1621 aaaagagcct ttcttaaggg ctcttttata attcattatt tttcaatgac aaatattagt
     1681 gaagttaatt tttatttaac cattgttatt attaatgcta ttttgccact tattaatcaa
     1741 cgaaaaagtt tcttctaggg aaactttttt atattatatt tcctgatagg aacttttatt
     1801 tttgatttag aaactctata atggacgtga aaggagattg accgatgctt ggagagaata
     1861 tctatttaca acgtactcag attggtatga cgcaagagaa tttgagtgat tatttgcatt
     1921 taacaaaaac tacaatttca aagtgggaaa ataatcaagc taaaccggat attgactatc
     1981 ttattttgat ggctaatctt tttgatatta gcttggatga cttagtcggt tatcaaaaaa
     2041 cattgtcaga tgatcaaaga aatcagctca ttaaggatct aaaaataaaa gcaaatgtat
     2101 tgtctgagag agatttcttt caggaagtta aggaattatc aaaacagttc cctaacgatt
     2161 ttaagactct gttgataatg attaatatgg tcttgagcaa cctaaccaac cttaatgatt
     2221 cagaaatgaa agagtggtca ctaagtacac taaataaaat tattagtaaa acagtagttg
     2281 agtctgacct ccagacggcg ataatgttca aaacggtcgt tctttttcac aaccttgaat
     2341 atgatgaagt gataaacctt taccagaata gtccctataa attaggtgaa gaattacttt
     2401 tagctaatag cttttttgca aaaggcgatg tttaccgagc taagcaagta ttacaggttg
     2461 aaatttatca gcagattcta cttgtatgcg agtatctatt gagtttatca acttttgaag
     2521 ttgaagaaaa caaaggcgat atttttgaaa ggcttgaaca tatgatagaa gcgtttcaat
     2581 taaactcttt gcatcccaac acagtcatca agtgttatta tagtttagct tgtcattata
     2641 gtaaaagtga tgatgataag gcattaggat acttaaaaca atgttttaaa tctttaaaac
     2701 aattgctcca aagatttgaa ttacacggtg atactttctt ttataccatc gatgattggt
     2761 tagagacaat acctactggt attgcaccac cgacgagtca attacaggta atagaacgag
     2821 ttgaggactt atttcaaaat tcacaattta aagaactatc agggcgtaaa gaatttcaac
     2881 aaatgattca aaaacttaac gatttaaaaa aggattacta atatgaatac agaaatacca
     2941 aatcaattat taccattcct agttccgttg attatgttgc aaggagcttt aattatcatt
     3001 tctctagtca aactaagtaa acttaagatg actaaacacc tttcaaagcc agtttggttt
     3061 ttggtcatta tatttcttaa tataattggg ccaattgcat ttcttatttt ggaagggaat
     3121 aatgaatgaa agctatctca tttcaaaacg ttacaaaatc ttttggtcct aaaaaaatat
     3181 taaataatgt ttcttttgat ttagaagaaa atatgattta tggattcgta ggaccaaatg
     3241 gagcaggtaa aacaacgaca ataaaaatga ttttaggatt gttaaaattc gatacgggtt
     3301 tcataacaat ttttggtaaa aaagttaatt ttggaagaac agataccaat caactgattg
     3361 gctatctccc agatgttccc gagtactacg attatatgac agctctagaa tatcttgatt
     3421 tatgttctgg tttagcgaga tcaaagcata agctttctaa caaagaacta cttaggtcag
     3481 ttggacttga cgataatcac caaaagattg ctacttattc tcgaggaatg aaacagcgtc
     3541 ttggattagc tcaagcactg gtccatgatc ctaaaattat tatttgcgat gaaccaacct
     3601 ctgcattaga cccgaaaggt cgccaagata ttttagatat tatttcaaat cttagaggag
     3661 aaaaaactgt gattttttct acccatattc tctctgatgt cgaaaaaata tgtgatcacg
     3721 ttctggtgtt aactaagtgt gggatttatt ctcttgaaga gttaaaagga aaaaaatcag
     3781 aggaaaatta ttcggttcgt attttaataa aagtgacaaa gtctgaagct aaggtactat
     3841 ctcataacta tcagatagaa aagaaagata atgagtatgc actgactctc aaaggctcta
     3901 aaatggataa caaagcagat ttactagctg gtttttacca agatttagtt tctttaaaga
     3961 ttagcccatc agctattgaa gtcattgaca atagtttaga ggagctttat ttggaggtta
     4021 ccaaatgaaa tctttagcag taatgttaaa aaaagaatgg atggagaatg ttaggacata
     4081 caaagttata tctattctga ttacttgttc aatatttggt atattaggtc ccttaactgc
     4141 acttatgatg cctgatatta tggcaggaat tttacctaaa aaactacagg gagcgatacc
     4201 agagcctacc tatattgatt cttatattca gtattttaaa aatatgaatc agcttggttt
     4261 agttatttta gtatttttat ttagtagtac actgacgcaa gaattttcaa aaggaacctt
     4321 aattaatcta gttacgaagg gattagccaa aaaagttata atccttgcta aatttatagt
     4381 aattaccctt ctatggacag tgtcatactt attaagtgtt gtcatccatt ttagttatac
     4441 gttatattac tttaataacg aaggaagtca taaattaatg gtttatggtg caacatggtt
     4501 tatcggtatc cttttcataa gcttaatact ctttttttca gttctttttc gtaaaacttt
     4561 aggggttttg ttaggttgtc tttttatggt ggtgttatgt gtagtatctg gattttttaa
     4621 aatcagtaaa agctataatc ctatgcttct tatccaaaaa caaggagaaa tgttaagtgg
     4681 aaagtttaaa gttcaagact taatggaacc gagcatatta gtgtttttgc ttattataat
     4741 gacgttatta cttagcatgt acatttttga aaaaagtgaa gtttaatttt gatgagatat
     4801 caataaggcg atagctactt tttgaaaaaa taaaaccgaa ttaagagtct tgctgctatt
     4861 tgacttatct agatcaatat ttccaactaa tatgatgtct tatgcatacc tcattgcaga
     4921 aatatacttt aaagtcaaaa taaggaaata tattatg
//
LOCUS       DATRNK010000095         4932 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.095, whole genome shotgun sequence.
ACCESSION   DATRNK010000095 DATRNK010000000
VERSION     DATRNK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.095"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(60..3077)
                     /gene="esaA"
                     /locus_tag="VBC52_001915"
     CDS             complement(60..3077)
                     /gene="esaA"
                     /locus_tag="VBC52_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HEO6883474.1"
                     /translation="MKRNNFRILWYIIAVALFLVAIAGLNLKLQGDHAKENKTTQSAT
                     NTKLNIALVNEDQNVSNGKESYNLGASYIKSIERDNSQNWSVVSRGTAQNGLNKGDYQ
                     LMVIIPNNFSQKLLDVNKANAEQTTISYKVNAKGNLALEKKATEKGKDIVSELNSHLV
                     NMYMASILSNLYTAQENVQAMVNVQSGNISNYQKNLLDSATNFQNIFPALVNQSSSSI
                     TANESLKKSLEASDNMFNDLVTTQTNTGKDLSRLIEQRHQDSISYEAFSASLLEMNNE
                     LLEKQLSDIITQAQKDQETLSSQLNSIMGDDNDNDHNHKENSSAYLNVARQKIQELSE
                     ALKSQDNVAKDQSEQLDKIVREGLASYFAKNNKDNITLLELLKSHSTNEKTLKDFKAK
                     VADFTNSLISSIPSLNLSELHLTQEEEKAIQFTSSDSEIIKKVSNERTLYLNTNLLNR
                     LFEARKNRDEAKNKVNQLKLPSSSTRTGEQIVSVESNNPDYRVDTWTVNGKQTRTLDP
                     SQNNNIIINSSYQGESSNSSITKPSYTITIGNHKQIVQNDDGKTQRSYLEAEATYQRT
                     LQEVIDAYNTTHGLVAKYYIISDGEEPQNLFDQFLNQSVNDTMVDLVKNGITKYLMDE
                     NTADAQQKVKDVMEEVENSQDELADQMAKVTETNVRLTDAIKKQLETLQSINMKVQNI
                     TQDQSKVNDSQKTTDQQLSDLKNQLDGLMTSAAGVKDMSKSNSQEADQVNQIFTSFNK
                     DVQDAKNSGNKLSTDATDLMANFQKELANNGDFVASFSKVFNTAYKNGVPNDILLNFL
                     SRPVAESASAVRATENTYRPFTWILLLEVVSLFTAYIFATQNLIKKLTDRYNVNRWLQ
                     TDFLNVIVISGLSLVIGLALGVISSRSLHVMPEYVPSWFLVMTLFSFLLIHSQYFFVK
                     NFKAVGMGLALFMIISFVYLSNAVGTVATVSGLPKLLKAINPLSILENQLSSYFDNVT
                     TGFIFLILVLLVDVAFIIMNIFITLNFEAKVKE"
     gene            complement(3159..3449)
                     /locus_tag="VBC52_001916"
     CDS             complement(3159..3449)
                     /locus_tag="VBC52_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEO6883475.1"
                     /translation="MAQIKLTPEELRSSAQKYTAGSQQVTEVLNLLTQEQAVIDENWD
                     GSAFDSFEAQFNELSPKITEFAQLLEDINQQLLKVADIIEQTDADIASQISG"
     gene            complement(3617..3907)
                     /locus_tag="VBC52_001917"
                     /pseudo
     CDS             complement(3617..3907)
                     /locus_tag="VBC52_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
     gene            complement(4024..4806)
                     /locus_tag="VBC52_001918"
     CDS             complement(4024..4806)
                     /locus_tag="VBC52_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HEO6883476.1"
                     /translation="MIPDSSFKVLSDMAYNIEESKAKSNGHLKIIEGSQIIDFHQKRS
                     FMVLEVNDDIDNGMQAMACQSLDESNHIVIAYSGTNAFNKKDILTDIDSVVLGKNNKL
                     LTCPSSITLREDGISLSCKNPSQITTSKLFAQKIKMNYPDATLWLTGHSLGGYLAMIV
                     ASEMRLSARVFNSPSPINNMSEAAASYVISHPEKYRSYRINKDYIGNFGINSSDDKLG
                     ISKRVNAKKQHGFYKKIFFHELTRFRLNQQGQIVDIDENKQD"
BASE COUNT         1529 a          839 c          770 g         1794 t
ORIGIN      
        1 actcatacag gataatatta gtagacaggc taaaaatcgt ttcaacttca tcaacaacct
       61 cactctttta ctttagcttc aaaatttaag gtgataaaaa tattcatgat aataaaagca
      121 acatccacaa gaagtactaa gataaggaaa ataaatccag ttgtcacatt atcaaaataa
      181 gacgataatt gattttcaag gatagatagt ggattaatag cttttagtaa ttttggaagt
      241 ccactaacag tcgctactgt gcctacggca tttgatagat agacaaaact aataatcata
      301 aacaaagcta aacccatacc aactgcttta aaatttttaa caaagaaata ctgactatga
      361 attaataaaa aactaaacag agtcataact aggaaccaag atggtacata ttcaggcatg
      421 acatgaagac ttctgcttga gataactccc agtgctaaac caatcacaag agagaggcct
      481 gaaatgacaa tgacatttaa aaagtctgtt tgaagccagc gattgacatt atacctatct
      541 gtcaatttct taataaggtt ttgagtcgca aagatatatg ctgtaaatag gctaacaact
      601 tctaataata gtatccaagt gaatgggcga taagtatttt cagttgccct aactgcagac
      661 gctgattcag cgacaggtcg tgataagaaa ttaaggagaa tatcatttgg cactccattt
      721 ttataagcag tattaaagac tttagagaaa gaagctacga aatcaccgtt attggccaat
      781 tctttttgga agttagccat taaatctgtt gcatccgtcg aaagtttgtt gcccgaattt
      841 ttagcatctt gaacatcttt attgaatgat gtgaatattt gattaacttg gtccgcctct
      901 tgactattag atttggacat atcttttacg ccagctgcag aggtcattag cccatctagc
      961 tggttcttca aatcagataa ttgttgatca gttgttttct gtgagtcgtt tactttagat
     1021 tgatcctgtg tgatgttttg gaccttcatg ttaattgatt gaagggtctc aagctgtttt
     1081 ttgattgcat ctgttagtcg tacatttgtc tcagttactt ttgccatttg atccgccaat
     1141 tcatcttgac tattttcaac ttcctccatg acatctttaa ctttctgttg cgcatcagca
     1201 gtgttttcat ccatcaaata cttagttata ccatttttga ccagatcaac catcgtatcg
     1261 ttaacacttt gatttaaaaa ttgatcaaat aaattttgcg gttcttcgcc atcagaaata
     1321 atgtagtatt tagctactaa gccgtgagtg gtgttatatg catcaattac ttcttgtaga
     1381 gttcgttgat acgttgcttc tgcctccaag taagaccgtt gggttttacc gtcatcattt
     1441 tgtacaattt gtttatgatt cccgatagtg attgtatagc ttggttttgt aatactagag
     1501 ttagatgatt ccccttgata actgctattg ataataatat tattgttttg gctggggtcc
     1561 aaggttcttg tttgcttacc attaaccgtc caggtatcta ccctgtaatc tggattattt
     1621 gattcaacag aaactatctg ctcaccagtt cgagtagaac tagatggcaa ttttaattga
     1681 ttaactttat ttttagcttc atctctattt ttcctagctt caaaaagacg atttaataaa
     1741 ttagtattta aatacagagt tctttcgttg cttacttttt taatgatttc cgaatcagaa
     1801 gatgtaaact gaatggcttt ttcttcttct tgggttaagt gcaactccga aaggtttagt
     1861 gaaggaatgc ttgaaatgag agaatttgtg aaatctgcta ccttagcttt aaaatctttc
     1921 aaagtcttct cattagtaga atgactcttc aataattcta ataaagtaat attatcttta
     1981 ttattcttag caaagtaact tgctagtccc tctctaacaa ttttatctag ttgttcactt
     2041 tgatctttag cgacgttatc ttgtgacttg agtgcttcag atagttcttg gattttttgc
     2101 cttgcaacat ttagataagc tgatgagttt tctttatgat tatgatcgtt atcgttatca
     2161 tcacccataa tactattgag ttgtgatgat aatgtttctt ggtctttttg tgcttgtgtg
     2221 ataatatcag acaattgctt ctctaataac tcgttattca tttctagtaa tgaggccgaa
     2281 aacgcttcat acgaaatgct atcttgatgg cgctgttcta ttaaccttga taaatctttt
     2341 ccagtatttg tctgggttgt caccaaatca ttaaacatgt tatcagaagc ttctaaagat
     2401 tttttcagtg attcgttagc agtaatggaa ctactagact ggttaacaag ggctggaaag
     2461 atattttgga aattagttgc agaatctaaa agattttttt ggtaatttga gatattacct
     2521 gactgaacat ttaccatggc ctgtacattt tcttgagctg tatataagtt acttaaaata
     2581 cttgccatat acatatttac taagtggcta tttaactctg aaacaatatc tttccctttt
     2641 tcagttgcct ttttctctaa tgctaaattc cccttggcat taaccttata agagatagtt
     2701 gtttgctctg catttgcttt attgacatca aggagttttt gagaaaagtt attagggatg
     2761 ataaccatta attgataatc acctttgttt aatccatttt gagcagtacc tcgactaaca
     2821 actgaccaat tttgactatt atcacgttcg atagacttaa tataactagc tcctaaatta
     2881 taactttctt taccattaga tacattttga tcttcgttaa ctaatgctat atttagttta
     2941 gtgttagtag ctgactgtgt tgttttattc tcttttgcat gatcgccttg aagcttaaga
     3001 ttaaggccag caatagctac taaaaagaga gcaacagcta taatatacca aagtattcta
     3061 aagttatttc ttttcataat tcttcctaaa atatatttta ttttcaacaa aataggaagg
     3121 ctttgccttc ctattcactt atagactatg atacgatact atccactaat ttgagaagca
     3181 atatctgcat ccgtctgctc aatgatatct gcaactttta ataattgctg gttaatatct
     3241 tcgagtaatt gagcaaattc tgtaattttt ggagataatt cattgaattg tgcttcaaaa
     3301 gaatcaaaag cagatccatc ccaattttcg tcaataactg cttgctcttg agtaagcaaa
     3361 ttaagaactt ctgttacttg ttgagaacca gctgtgtact tttgtgctga tgagcgaagt
     3421 tcttctggtg ttaatttaat ttgtgccatt gaatacccct tcttaaatat agcgctatca
     3481 cgtataaata ttatatcata aaaatgagaa aatacctaat atttacctta tttaatacaa
     3541 aaaagtataa aaaaaatctc tggaatatca tttaagtaaa accagagact ttttttattt
     3601 acaatctttt aaaaaattat ccaccaattt gtgaagcgat atcagagtct gtttgctcaa
     3661 caatatcagc aactttaagc aattgttgat taatgtcttc aagtaattga gcaaattctg
     3721 tgattttagg tgacaactca ttgaattgtg cttcgaaaga atcaaaagcg ctaccatccc
     3781 agttttcatc aataacagct tgttcttgag taagtgagtt aaggacttct gttacttgtt
     3841 gagaaccagc agtatatttt tgtgctgatg cgcgaagttc ttctggtgtt aattaaattt
     3901 gtgccataaa ttttctcctt tttctataaa cacttttgca acaactgcat aattattata
     3961 acatattttt taaaatatat tcaaatttac tgcattattt aaaattattt ttaatgttat
     4021 tgtttaatcc tgtttatttt catctatatc aacaatctgt ccttgttgat ttaatcgaaa
     4081 tcgtgtcaat tcgtgaaaga aaatcttttt ataaaaacca tgttgcttct tagcattgac
     4141 tcttttagaa atacctaatt tatcatctga actattaata ccaaaattac caatgtaatc
     4201 tttattaata cggtaactac gatacttctc tggatgtgaa attacatagg atgcggctgc
     4261 ttcagacata ttgttaatgg gactagggga attaaaaaca cgcgctgata atctcatttc
     4321 tgaagccaca atcatggcca aataaccacc tagagaatgc cctgttaacc agagggttgc
     4381 atcaggatag ttcatcttaa ttttttgtgc aaatagttta gatgttgtaa tttgacttgg
     4441 atttttacaa gacagagaaa tgccatcctc tctcagggta atacttgaag gacaagttag
     4501 caatttattg ttcttcccca atacaactga atcgatatct gttaagatat ctttcttatt
     4561 aaaagcgttc gttcctgaat atgctataac aatatgatta ctctcatcca aagactgaca
     4621 tgccatagcc tgcataccat tatcgatatc atcattaacc tctaatacca taaaagagcg
     4681 tttttgatgg aaatcaataa tttgactacc ttctataatt ttaaggtgtc cattactttt
     4741 tgctttggat tcttcaatat tatatgccat gtctgatagc actttaaaac tactatctgg
     4801 aatcattcat tttcctctct attcaaagac cagatgatat ctaactcatc aatagactga
     4861 ataggttcct ttttttctct aggtctgaga tgaaaacttt tagaatgaca aataatacct
     4921 gaaatactat ca
//
LOCUS       DATRNK010000096         4875 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.096, whole genome shotgun sequence.
ACCESSION   DATRNK010000096 DATRNK010000000
VERSION     DATRNK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.096"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            306..1922
                     /gene="nikA"
                     /locus_tag="VBC52_001919"
     CDS             306..1922
                     /gene="nikA"
                     /locus_tag="VBC52_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HEO6883477.1"
                     /translation="MRRNILLSITCLLMVTLTACHSQDSKSHKLNSDKLTLAWGEDFG
                     DVNPHRYNPDQFVIQDMVYEGLVRYGDNGKIEPALAESWSISQDGKTYTFKLRNAKYS
                     DGSNFNAANVKRNFDSIFSKSNRGNHNWFNLTNQLENYRALNQSTFEIKLKQAYSATL
                     YDLSMIRPIRFLSDNAFPKGDDTTKKNVKKPIGTGQWVVKSKKQNEYITFKRNENYWG
                     KKPKLKEVTVKVIPDAQTRALAFESGDVDLIYGNGIIGLDTFAQYTKDKKYVTAVSQP
                     MSTRLLLLNAKESIFQDKKVRQAMNHAIDKVSIAKNTFRGTEKPADTIFSKSTPHSDA
                     KLNTYSYNVDKANQLLDQAGWKLGKDKVREKDGKALTLRLPYIATKATDKDLVTYFQG
                     EWRKIGINVSLIAMEEDDYWANAKKGNFDMMLTYSWGAPWDPHAWMSALTAKADHGHP
                     ENIALENLATKTEMDRLIKSALVDPKEENVDRDYKKVLELLHDEAVYIPLTYQSVISV
                     YRKGDFKTMRFAPEENSFPLRYIEKNNVSK"
     gene            1909..2853
                     /locus_tag="VBC52_001920"
     CDS             1909..2853
                     /locus_tag="VBC52_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6883478.1"
                     /translation="MYLSSSIIKKILSAFLALFFISLLTFILIKLSTVNPAENYLRLS
                     KISVSPEALKEAEHYLGLDKPLWKQYWLWFQKALTGDFGYSYVLRLPVLDLVLQRFLA
                     TLFLGTSAFLLIVTISTPLGVWAGLHESARSDHLIRFLSFSSVSMPSFWVAYLLMLLF
                     SAKLNLLPVSGGNDLQSLILPSITLSFSTVGQYIALIRKAISQENRSLNVENARLRGV
                     KERYIVTHHLLRNALPAIMTALSLTWVYLLTGSIIVEEIFSWNGIGRLFVTSLRTSDL
                     PVIQACMLIFGTLFLANNFMTQCLMNWVDPRLRKSREK"
     gene            2853..3659
                     /locus_tag="VBC52_001921"
     CDS             2853..3659
                     /locus_tag="VBC52_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6883479.1"
                     /translation="MSKRIYFSSSLLVLLVISAIFAPILSSFDPQYVDLSQKLLAPNN
                     VHLLGTDQLGRDVLSRLLYGARYSLFLAIIISLLELTIGMFVGLIVGWYQGKLENLFL
                     WIANIILAFPSFLLSLATVGILGHGLGNLIFAIVFVEWVYYAKLMTNLVKSAKKEPYV
                     INAQIMGLSVWHILRKHIFPFVYQPILVMVLMNIGNIILMISGFSFLGIGVQPNVTEW
                     GMMLHDARGYFRTATWMMLSPGIAIFLTVFSFNTLGDAIDKKDWKRQWNS"
     gene            3647..4435
                     /locus_tag="VBC52_001922"
     CDS             3647..4435
                     /locus_tag="VBC52_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6883480.1"
                     /translation="MEQLEIRNLSLQIGEVPVLRDFSCKIDMGESLTIIGESGSGKTL
                     LAKLLVGHIPQGMTVRGNIFFKGVDLGKLTVKQWQKLRGRDIAYLVQNPMSMFNPFQK
                     IEAHILETILSHEKCSKRVALSKALEWMKRLNLDDAVSLLKKYPFELSGGMLQRIMLA
                     TILSLDPQVIILDEPTSAVDCHNCSTISAILQELQNNGKTLITVTHDYQLARDLGGQL
                     LVISEGEVVEQGQTQAILSNPQHNYTKALTVQMEYEGDILNVGL"
     gene            4422..>4875
                     /locus_tag="VBC52_001923"
     CDS             4422..>4875
                     /locus_tag="VBC52_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6883481.1"
                     /translation="MLVCNHVGKTFGRQEVLKDCHFHLKRGEIIGIMGKSGSGKSSLA
                     RLIIGLDSPTCGSIHFQGKNYTPKDGKAQIILVFQDALSSVNPYFSIEEILNEAFYGK
                     KTTFELCQILEAVGLDGTYLKYKARQLSGGQLQRVCIARALLLKPKIII"
BASE COUNT         1574 a          702 c          982 g         1617 t
ORIGIN      
        1 tattatatgt gtttcttttg aaaaatagct ttttatctgt attaaccttc ttaaagacta
       61 atggatgtgc gatgtgtttt aagtaacaga tgagaataag gaaaaatgaa attggcgata
      121 tggtatgtga ctattatgat tattctttag caataatcta ttaaaatgag ttcgcaggga
      181 ataagtaaat cttgtatcgt tgtagatttt tttattttaa gattcttgac aagacattac
      241 taaaatgatt taattaagtt tgcttaaaaa aataattaat taaacttaaa agaaagttgg
      301 taaaaatgag acgtaacata ttattgagta ttacttgttt attgatggtg actctgactg
      361 catgccattc acaggatagc aaaagtcata aactaaacag tgataaactc acgcttgctt
      421 ggggagaaga ctttggtgat gttaatccgc atcgctataa ccctgatcag tttgttatcc
      481 aggatatggt ttatgagggg ttggtacgat atggtgacaa tgggaaaatt gaaccagctt
      541 tagcggaaag ctggagcatt agtcaagatg gaaaaaccta cacttttaaa ttgagaaatg
      601 ctaaatattc tgatggcagt aactttaatg ctgcgaatgt caaacgtaat tttgatagta
      661 ttttttcgaa gtctaataga ggtaatcata attggtttaa tttaacgaat caattggaaa
      721 attatcgtgc cttgaatcaa agtacttttg aaattaaact gaaacaagct tatagtgcta
      781 ctttatatga tctctctatg attcgtccaa ttcgtttttt atctgataac gctttcccta
      841 aaggtgatga tacaactaag aagaatgtaa aaaaaccaat tggaacgggt caatgggttg
      901 tgaaaagtaa aaagcaaaat gaatatatta ccttcaaacg taatgagaat tactggggaa
      961 agaaaccaaa attaaaagaa gtaaccgtaa aagttatacc tgatgctcag acgcgtgctt
     1021 tagcatttga atcaggtgac gttgatttga tctatgggaa tggcattatt ggtttagaca
     1081 cttttgccca atatacgaag gacaagaaat atgtcacagc tgtttctcaa cctatgtcaa
     1141 caagactttt gttgctaaat gctaaggaaa gtattttcca agataaaaaa gttcgtcagg
     1201 caatgaatca tgctattgac aaagtttcta ttgctaagaa cacttttaga ggaacagaaa
     1261 agccagcaga cactattttt tcaaaatcaa cacctcactc agatgctaag ttaaatactt
     1321 atagctataa tgttgataaa gcaaatcagc tattggatca agctggttgg aagttgggaa
     1381 aagataaggt tcgtgaaaaa gatggtaaag ctctaacctt acgtcttcct tatatcgcca
     1441 ctaaggcaac tgataaagac ctggtaactt atttccaagg agaatggcgt aaaatcggta
     1501 ttaatgtttc tcttattgct atggaagaag atgattattg ggctaatgct aaaaagggta
     1561 attttgatat gatgttaacc tattcttggg gagcaccatg ggaccctcat gcttggatgt
     1621 cggcattaac tgccaaggca gatcatggac atccagaaaa tatagcttta gaaaatctag
     1681 caactaaaac tgaaatggat agacttatta agtcagctct agtagatcct aaggaagaaa
     1741 atgttgatag agactataag aaggttcttg aattattgca tgatgaggct gtatatatcc
     1801 cattaactta tcaatcggtg atttctgttt ataggaaagg tgattttaaa accatgcgct
     1861 ttgcgccaga agaaaattca ttcccattac gctatataga aaaaaataat gtatctaagt
     1921 agttcgatta tcaaaaaaat attgtcagct tttttggcat tgtttttcat atcacttcta
     1981 acatttattc ttatcaaact atcaacagtt aatccagcag aaaattacct tcgtttatca
     2041 aaaataagtg ttagtccaga agcactaaaa gaagcagagc actatctagg tttagataag
     2101 ccattatgga agcagtactg gctgtggttc caaaaagcat taacaggaga ttttggatat
     2161 tcttatgtgc tcaggttgcc agtattagat ttggttttac aacggttttt ggctacttta
     2221 tttctaggca cgagtgcctt cttgctaatt gttaccattt ctaccccgtt aggtgtgtgg
     2281 gctggcttac atgaatctgc tcggagtgat cacttgattc gatttttgag tttttctagt
     2341 gtgtctatgc caagtttttg ggttgcttac ttgttgatgc tgctattttc ggcaaagtta
     2401 aatcttctgc ctgtttcagg aggtaacgat ttacaaagtt taattttgcc aagtattacc
     2461 ttaagttttt ctaccgtagg tcaatatata gcccttattc gaaaagctat tagtcaagaa
     2521 aatagaagtt taaatgtgga aaatgctcga ttaagaggag taaaagaacg ttatattgtc
     2581 acacatcacc tccttagaaa tgccttacct gcgataatga cggcacttag cttgacttgg
     2641 gtttacttat taacaggatc aattattgtt gaagaaattt tttcatggaa tggaatagga
     2701 cgtttatttg tgaccagtct aaggacttca gatcttccag taatacaagc ttgtatgcta
     2761 atttttggaa ccttattttt agctaataat tttatgacac agtgtcttat gaattgggtt
     2821 gatcctcggt tacgaaagtc gagggagaaa taatgtcaaa aagaatttat ttttcaagta
     2881 gcttactggt attgttagtg attagtgcca tttttgcccc tattctatct agctttgatc
     2941 cacaatacgt agatttatca caaaaattat tggctcccaa taatgttcat ttgttaggga
     3001 ctgaccaatt gggtagagat gtattatctc gcttgcttta tggtgctaga tattcactgt
     3061 ttttagctat tattattagc ttattggaat taacgattgg tatgtttgtg ggtcttattg
     3121 ttggttggta tcaaggaaag ttagaaaacc tctttttatg gatagctaat attattttag
     3181 cttttccgag ctttttgctc tctcttgcaa ccgtggggat cttaggtcat ggtctaggga
     3241 atttaatttt tgcaattgtc tttgtggaat gggtttacta tgctaaatta atgaccaatt
     3301 tagttaagag tgcaaaaaaa gagccttatg tgataaatgc gcaaattatg ggactttcgg
     3361 tctggcatat tttaagaaaa catatttttc cttttgtgta tcagccaatc ctcgttatgg
     3421 tccttatgaa tataggaaat attattttaa tgatttctgg cttttctttc ttagggattg
     3481 gtgtgcaacc taatgtcaca gaatggggaa tgatgttgca cgatgctaga ggatatttta
     3541 ggacagctac ttggatgatg ttatctcctg gaattgcgat ttttttaaca gtattttcct
     3601 ttaatacttt aggcgatgct atagataaaa aagattggaa acgacaatgg aacagttaga
     3661 aataaggaat ttgtcattac agataggaga ggtccctgta ctaagagatt ttagttgtaa
     3721 aatagatatg ggggaatcgc taactattat tggtgagagt ggttcaggga aaaccctctt
     3781 ggcaaaatta ttggttggtc atattccaca aggtatgaca gttagaggaa acatattttt
     3841 taagggagtt gatttaggta aactaactgt aaagcagtgg cagaaattaa ggggacgaga
     3901 tattgcctat ttagtgcaaa atcccatgtc tatgtttaat ccttttcaaa aaattgaagc
     3961 gcatattttg gaaacaatcc ttagtcatga gaaatgttca aagagagtag ccttatctaa
     4021 agcgttagaa tggatgaaac gtttaaattt agatgatgcg gtatccctgt taaaaaaata
     4081 cccctttgag cttagtggag gaatgctaca aagaattatg ttagccacta tattatctct
     4141 tgatcctcag gtaatcatat tagatgagcc gacctcagcg gttgattgcc ataattgttc
     4201 tactatatca gctattttgc aggaattgca aaataatgga aaaaccttga ttactgtaac
     4261 gcatgattat cagttagcta gagacctggg ggggcaatta ctagttataa gtgagggaga
     4321 agttgttgaa cagggacaaa cccaagctat tttaagcaat cctcaacata actatacgaa
     4381 agcactgaca gtgcaaatgg agtatgaagg agatattctt aatgttggtt tgtaatcatg
     4441 tcggaaaaac atttggacga caagaggttt taaaggactg tcattttcat cttaaaagag
     4501 gagaaataat tggtataatg ggtaaaagtg gcagtggaaa aagtagcctt gcccgactca
     4561 ttataggact agatagtcct acttgtggtt cgatacattt tcaaggaaaa aattacacac
     4621 ctaaagatgg taaggcgcag attatccttg tttttcaaga tgcgcttagt tcggtcaatc
     4681 catattttag tattgaggaa attttgaatg aagcttttta tggaaaaaaa acaacttttg
     4741 aattatgcca aatattagaa gcggtgggtt tagatggaac ttatctaaaa tataaagcta
     4801 gacaacttag tggtggtcag ctacagagag tttgtatcgc tagagcttta ctcttaaagc
     4861 ctaaaattat tattt
//
LOCUS       DATRNK010000097         4746 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.097, whole genome shotgun sequence.
ACCESSION   DATRNK010000097 DATRNK010000000
VERSION     DATRNK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.097"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            150..1394
                     /locus_tag="VBC52_001924"
     CDS             150..1394
                     /locus_tag="VBC52_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO6883482.1"
                     /translation="MSKRQNLGISKKGAIISGLSVALIVAVGGFLWVQSQPNKSAVKT
                     NYKVFNVREGSVSSSTLLTGKAKANQEQYVYFDANKGNRATVTVKVGDKITAGQQLVQ
                     YDTTTAQAAYDTANRQLNKVARQINNLKTTGSLPAMESSDQSSSSSQGQGAQSTSGAT
                     NRLQQNYQSQANASYNQQLQDLNDAYADAQAEVNKAQKALNDTVITSDVSGTVVEVNS
                     DIDPASKTSQVLVHVATEGKLQVQGTMSEYDLANVKKDQSVKIKSKVYPDKEWEGKIS
                     YISNYPEAEANNNDSNNGSSAVNYKYKVDITSPLDALKQGFTVSVEVVNGDKHLIVPT
                     SSVTNKDNKHFVWVYNDSNRKISKVEVKIGKADAKTQEILSGLKAGQIVVTNPSKTFK
                     DGQKIDNIESIDLKSNKKSEVK"
     gene            1397..2107
                     /locus_tag="VBC52_001925"
     CDS             1397..2107
                     /locus_tag="VBC52_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6883483.1"
                     /translation="MTTNRKELIRLHQIVKSYQNGDQKLQVLKNIDLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDSPTSGDYSLNGKRVEELSQTKLAQVRNKEIGFVFQQFFLL
                     SKLTALQNVELPLIYAGVAPKKRKNLAKQFLDKVELRERMNHLPTELSGGQKQRVAIA
                     RALVNSPSIILADEPTGALDTKTGEQIMQFLTELNQEGKTIIMVTHEPEIADYATRKI
                     VIRDGEITADTTDSIRID"
     gene            2122..3369
                     /locus_tag="VBC52_001926"
     CDS             2122..3369
                     /locus_tag="VBC52_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6883484.1"
                     /translation="MENWKFALSSILGHKMRAFLTMLGIIIGVASVVLIMALGKGMKD
                     SVTNEITKSQKNLQIYYKTKEDQKNEDNFGAQGAFMQSSDTNRKEPIIQESWLKKIAK
                     EVDGVSGYYVTNQTNAPVAYLEKKAKTVNITGINRTYLGIKKFKIKSGRQFQEEDYNQ
                     FSRVILLEEKLAQRLFQTNEAALNKVVTVKNKSYLVVGVYSDPEAGSGLYGSNSDGNA
                     ILTNTQLASEFGAKEAENIYFHLNDVSQSNRIGKEIGKRLTDISHAKDGYYDNFDMTS
                     IVKSINTQVGIMTGVIGAIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRRK
                     ILAQFLIESMVLTILGGLIGLLLAYGGTMLIANAQDKITPSVSLNVAIGSLIFSAFIG
                     IIFGLLPANKASKLNPIDALRYE"
     gene            3498..3770
                     /gene="rpsP"
                     /locus_tag="VBC52_001927"
     CDS             3498..3770
                     /gene="rpsP"
                     /locus_tag="VBC52_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEO6883485.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQVTIKEERVLEWLSKGAQPSDTVRNLLSKAGVMTKFHDQKFSK"
     gene            3780..4022
                     /locus_tag="VBC52_001928"
     CDS             3780..4022
                     /locus_tag="VBC52_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEO6883486.1"
                     /translation="MDTIENLIIAIVKPLISQPDQLTIKIQDGPEFLEYHLDLDTQDI
                     GRVIGKKGRTITAIRSIVYSVPTQGKKVRLIIDEKE"
     gene            4154..>4746
                     /locus_tag="VBC52_001929"
     CDS             4154..>4746
                     /locus_tag="VBC52_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6883487.1"
                     /translation="MLFDFLEEKIKLKAYLMTALLDDGEINLDHFKTEYALSEKILQG
                     LISELQILYSDFYLVVKSRTMVLFHYETLSKLETLHTIYRESNMLQFLAYFIAPQNVS
                     FSEFTAKKFISVASAYRIKKNCKLYLESVKLSIQKNKVTGPEYQIRLLIALLQYHFGF
                     IIYPFDATSEVMIRDFIEYSNFKGCDYDLKDLSEEYY"
BASE COUNT         1686 a          718 c          913 g         1429 t
ORIGIN      
        1 agtaaagtac tttcaatttt gtaagttctt tctatgagca aaatagtata cttaatgagt
       61 aaaaatatta tttttaaaga attttcttga caatattatt taaaaataat acacttgttc
      121 tatattaata atacaaacag gagatggaca tgagtaaacg acaaaattta ggaattagta
      181 aaaaaggagc aattatatca gggctctcag tggcactaat tgtagcagta ggtggctttt
      241 tatgggtaca atctcaacct aataagagtg cagtaaaaac taactacaaa gtttttaatg
      301 ttagagaagg aagtgtttcg tcctcaactc ttttgacagg aaaagctaag gctaatcaag
      361 aacagtatgt gtattttgat gctaataaag gtaatcgagc aactgttaca gttaaagtgg
      421 gtgataaaat cacagctggt cagcagttag ttcaatatga tacaacaact gcacaagcag
      481 cctacgacac tgctaatcgt caattaaata aagtagcgcg tcagattaat aatctaaaga
      541 caacaggaag tcttccagct atggaatcaa gtgatcaatc ttcatcatca tcacaaggac
      601 aaggggctca atcgactagt ggtgcgacga atcgtctaca gcaaaattat caaagtcaag
      661 ctaatgcttc atacaaccaa caacttcaag atttgaatga tgcttatgca gatgcacagg
      721 cagaagtaaa taaagcacaa aaagcattga atgatactgt tattacaagt gacgtatcag
      781 ggacagttgt tgaagttaat agtgatattg atccagcttc aaaaactagt caagtacttg
      841 tccatgtagc aactgagggt aaactccaag tacaaggaac gatgagtgag tatgatttgg
      901 ctaatgttaa aaaagaccag tctgttaaaa taaaatctaa ggtctatcct gacaaggaat
      961 gggaaggtaa aatttcatat atctcaaatt atccagaagc agaagcaaac aacaatgact
     1021 ctaataacgg ctctagtgct gtaaattata aatataaagt agatattact agccctctcg
     1081 atgcattaaa acaaggtttt actgtatcag ttgaagtagt taatggagat aagcacctta
     1141 ttgttcctac aagttctgtg acaaacaaag ataataaaca ctttgtttgg gtatacaatg
     1201 attctaatcg taaaatttcc aaagttgaag tcaaaattgg taaagctgat gctaagacac
     1261 aagaaatttt atcaggtttg aaagcaggac aaatcgtggt tactaatcca agcaaaactt
     1321 tcaaggatgg gcaaaaaatt gataatattg aatcaataga tcttaagtct aataagaaat
     1381 cagaggtgaa ataatcgtga caactaatcg aaaagaactg attagattgc atcagattgt
     1441 taaaagttat caaaatgggg atcaaaaact tcaagtctta aagaacattg atttgacggt
     1501 ttatgaaggt gaatttcttg caatcatggg gccatctggt tcggggaaat ccacactaat
     1561 gaatattatt ggcttgttgg attccccaac ttcaggagat tatagtttaa atggtaaacg
     1621 tgtcgaagaa ctaagtcaaa caaaattagc tcaagttcga aataaggaaa tcggttttgt
     1681 ctttcaacaa tttttcttgt tatctaaact aacggctttg caaaatgttg aattaccctt
     1741 aatttatgcg ggtgtagctc ctaaaaagcg taaaaattta gcaaaacaat ttttagataa
     1801 agttgaatta agagagcgta tgaaccatct gccaacagaa ttatctgggg gacagaagca
     1861 acgtgtagca atcgcgcgtg ctttggtgaa tagcccatct attattctag cagatgagcc
     1921 gactggtgcc ttggatacaa agacgggtga gcaaattatg cagttcctaa cggaattaaa
     1981 tcaagaaggt aaaaccatta tcatggtaac tcatgaacca gaaatcgccg attatgcgac
     2041 acgtaaaatt gttattcgtg atggcgaaat tacagcagat actactgata gtattagaat
     2101 tgattaaggg gagattcgat tatggaaaat tggaaatttg ccctatcatc gattctaggg
     2161 cacaagatgc gtgcttttct gaccatgtta gggattatca ttggggttgc atcagttgtg
     2221 ttaattatgg ccttaggtaa gggcatgaaa gattcggtta caaatgagat tacaaagtct
     2281 cagaagaacc ttcagattta ttataaaaca aaagaagacc aaaaaaatga ggataacttt
     2341 ggagctcaag gggctttcat gcaaagtagt gataccaacc gtaaggaacc aattattcaa
     2401 gaatcatggt taaaaaagat tgctaaggaa gtagatggtg ttagtggcta ctatgtaact
     2461 aatcaaacaa atgctccagt agcttacctt gaaaaaaagg caaagacggt taatataaca
     2521 ggaattaacc gcacttacct tggtatcaaa aagttcaaaa ttaaaagtgg tcgtcaattc
     2581 caagaggagg attataacca attctcgaga gttattttac tagaagaaaa attagctcag
     2641 agattatttc agacaaatga agcagcgtta aataaagtgg tgactgtaaa aaataaatct
     2701 tacttagtag tgggggttta ttcggatcca gaagctggtt caggtttata tggaagtaat
     2761 tctgatggga atgctatttt aaccaataca cagttagctt cggagtttgg tgctaaagaa
     2821 gcagaaaaca tatattttca tcttaatgat gtatcgcaat ccaatcgcat tggtaaagaa
     2881 ataggcaaac gtttaacaga cattagtcat gctaaggatg ggtactatga taattttgac
     2941 atgacatcta tagttaaatc tatcaatacc caagttggta ttatgacagg tgttattgga
     3001 gcgattgcag caatttctct cctagttggt ggtatcggag tgatgaatat tatgttagtc
     3061 tctgtaactg agcgcacgag agaaattggc cttcgtaaag cccttggggc aactcgacgt
     3121 aaaattttag ctcaattttt aattgaatca atggtcttaa cgattttggg tgggttaatt
     3181 gggttattac tagcctatgg aggtacgatg ttaattgcta atgcgcaaga taagattact
     3241 ccttctgtgt cacttaacgt ggctataggc agtttaattt tctcagcatt tattggaatt
     3301 atatttggtt tattacctgc taataaagct agcaaattaa acccaattga tgctttacga
     3361 tatgaataaa aaaacatatg ctctgtcaag attttttctt gacagagttt gtatttatgt
     3421 tatacttagt aaggtaaagt tgttagttaa gctatcaacg accaaatata aagaaaaaga
     3481 aaagagacta tttaaaaatg gcagtaaaaa tccgtttaac tcgtatgggt tctaagaaga
     3541 aacctttcta ccgtatcaat gttgcagatt cacgtgcacc acgtgacgga cgttttattg
     3601 aaactgttgg aacatacaac ccattagttg cagaaaacca agttacaatc aaagaagaac
     3661 gtgttcttga atggttatca aaaggtgcac aaccttcaga tacagttcgt aaccttcttt
     3721 caaaagctgg agttatgact aaattccacg atcaaaaatt ctcaaaataa taaaagccta
     3781 tggataccat tgaaaatctc attattgcta ttgtgaaacc tttgatttca caaccagatc
     3841 agttaaccat aaaaattcag gatggcccag aatttttaga gtaccatctt gatttagata
     3901 cccaagacat cggtcgtgtt atcggcaaaa agggtcgtac aattacagcg ataaggtcga
     3961 ttgtctattc ggtaccaaca caagggaaaa aagtaagact catcattgat gagaaagaat
     4021 aatttattct aaaagatagg aacttcctgt cttttttgtt atatctacac aaaaattgtg
     4081 agttttttag tcttttagaa tataatggat gtgaaaggga attttctgat tttttattaa
     4141 ggaggataag atattgctat ttgattttct cgaagaaaaa ataaaactga aagcatacct
     4201 gatgaccgcc ttacttgatg atggagaaat taaccttgat cactttaaaa cagaatatgc
     4261 tttgagtgaa aagattttac aaggtcttat ttcggaacta caaatacttt attctgattt
     4321 ctaccttgtt gtcaaaagta gaacaatggt attgttccat tacgaaacac tttctaagct
     4381 agagactttg cataccattt atcgagagtc taatatgtta cagtttttgg catattttat
     4441 tgctcctcaa aatgtcagtt tttctgaatt cacggctaag aaattcatat ccgtcgcaag
     4501 tgcctaccga ataaagaaaa actgtaaact ttatttggag agtgttaagt tatcaataca
     4561 aaaaaataaa gtgacaggtc cagaatatca gattagactg ctgattgctt tgttacagta
     4621 tcattttggt tttattattt atccttttga tgctacaagt gaggtaatga taagggattt
     4681 tattgaatac tcgaatttta aagggtgtga ctacgacctt aaagacttat ctgaagaata
     4741 ttacta
//
LOCUS       DATRNK010000098         4693 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.098, whole genome shotgun sequence.
ACCESSION   DATRNK010000098 DATRNK010000000
VERSION     DATRNK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.098"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            339..1370
                     /locus_tag="VBC52_001930"
     CDS             339..1370
                     /locus_tag="VBC52_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6883488.1"
                     /translation="MKLSKQLYKSLPLLTVVLCVGALQQNVEAKDKHYKTTSHVETQY
                     VSTSSKKILPFTHNKQIKVGSLDNLGRATYSHIQLRDADEPKIKRERLTYNPTGWHNY
                     KFTTEKGKTTWLMDRGHLVGYQFSGMNNVPENLVTMTKYLNTGFSENNPDGMLYYENR
                     LDSWLANHKNFWLDYKVTPIYEGNNLVPSRVELQYVGIDKQGKLLEIKLGGGKEQVDE
                     YGVTTVTLENTSPLAKIDYKTGMLIKEDGKQAEEGEDPNSDADENEAAIESASDIEEN
                     TNTNTSESDTNNVAPQNRIVYVANKGRSNTYWYSLENIKNANTANIVQMTEQEALNQH
                     KHHSTTEAQ"
     gene            complement(1581..2393)
                     /locus_tag="VBC52_001931"
     CDS             complement(1581..2393)
                     /locus_tag="VBC52_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO6883489.1"
                     /translation="MLLDMLSYDFMQRALLAVVAISIFAPILGIFLILRRQSLMSDTL
                     SHVSLAGVALGVVLGISPTWSTIFVVTLAAVVLEYLRTVYKHYMEISTAILMSMGLAI
                     SLIVMSKAHNVGNVSLEQYLFGSIITIGKEQVIALFVIALITFILTILFIRPMYILTF
                     DEDTAFVDGLPVRTMSILFNVVTGIAIALTIPAAGALLVSTIMVLPASIAMRLGRNFK
                     TVIFLGMLIGFVGMVAGIFLSYYWETPASATITMIFIGIFLLVSLVGLLRKR"
     gene            complement(2383..3093)
                     /locus_tag="VBC52_001932"
     CDS             complement(2383..3093)
                     /locus_tag="VBC52_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO6883490.1"
                     /translation="MRYITVSGLTFQYDSDPVLEGVNYHLDSGEFVTLTGENGAAKST
                     LIKATLGILTPKVGTVNISKENKEGKKLRIAYLPQQIASFNAGFPSSVYEFVKSGRYP
                     RNGWFRRLTKHDEEHIRVSLEAVGMWDNRHKKIGSLSGGQKQRAVIARMFASDPDIFV
                     LDEPTTGMDAGTTEKFYELMHHNAHKHGKSVLMITHDPDEVKGYADRNIHLVRNQSLP
                     WRCFNVHTNEMEVESDAT"
     gene            complement(3096..3539)
                     /locus_tag="VBC52_001933"
     CDS             complement(3096..3539)
                     /locus_tag="VBC52_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEO6883491.1"
                     /translation="MTVLEQKLDHLVSQILLKAENQHELLFGTCQSDVKLTNTQEHIL
                     MLLSQEQLTNSDLAKKLNISQAAVTKAVKSLISQDMLKANKDSKDARITYFELSELAK
                     PIADEHTHHHDNTLGVYGRLVNHFSKDEKVVLERFLDLFSRELEG"
     gene            complement(3625..4476)
                     /locus_tag="VBC52_001934"
     CDS             complement(3625..4476)
                     /locus_tag="VBC52_001934"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEO6883492.1"
                     /translation="MKIFEKAPAKLNLGLDIKGRCDDGYHELAMIMVSIDLNDYVTIS
                     ELKEDCIVIDSDSSKMPLNNDNDVFKAADIIKNQYGINKGVHIRLEKSIPVCAGLGGG
                     STDAAATIRALNRLWNLQMDYDEMVAIGFKIGSDVPYCLGGGCSLVLGKGEIVKPLPT
                     LRPCWIVLVKPDFGISTKSIFRDIDCKSISRVDIDLLKSAILSSDYQLMVKSMGNSLE
                     DITITKNPVISTIKERMLNSGADVALMTGSGPTVFSMCSTEKKADRVFNSMKGFCKEV
                     YKVRLLR"
     gene            complement(4616..4689)
                     /locus_tag="VBC52_001935"
     tRNA            complement(4616..4689)
                     /locus_tag="VBC52_001935"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4653..4655),aa:Asn,seq:gtt)
BASE COUNT         1747 a          911 c          667 g         1368 t
ORIGIN      
        1 taaaaacaac aaatatcgtc tgtctaacac caacgaattt attctaacct ttacatgtta
       61 taaatcaaga aaatatgatt agaatacctt aactttgtaa tcaaaatcag ctaaagaaat
      121 aatccattaa aaaatctaat aaaataacaa atcatataaa aattattatg taataacagt
      181 atatctataa catttaactt taataactat ctagttaaat aacataatct ccaaaaaaaa
      241 ttaactagtt atgttttttt attacttttt cccaataaaa tgataaaatt atacttgtga
      301 aaggccttca caaatatcat ttagaagggt ttaggtttat gaaattatct aaacaattat
      361 acaaatcact cccactatta actgtcgtgc tatgcgttgg ggcattacaa cagaatgttg
      421 aagctaagga taaacattat aagacgacat cccatgttga gacacagtat gtaagtactt
      481 cttcaaaaaa aatcttaccg tttactcata ataagcaaat caaggtaggt tcattagata
      541 acttaggaag agcaacatat tctcacatac aattaaggga tgcagatgag cctaagataa
      601 aaagagaaag actgacgtat aatccaaccg gttggcacaa ctacaaattt actactgaaa
      661 agggtaaaac aacttggctt atggaccgag gtcacctcgt tggttatcaa ttttcaggaa
      721 tgaacaatgt tcctgagaac ctagttacta tgaccaaata tctaaataca ggatttagcg
      781 aaaataatcc tgatggtatg ttatattacg aaaatagatt agatagttgg ttggcaaatc
      841 ataaaaattt ctggcttgat tacaaagtga ctccaatata cgaaggaaac aatttagtac
      901 ctagccgagt tgaacttcag tatgttggaa ttgataaaca aggaaaactt ctagaaatca
      961 aattaggcgg cggcaaagaa caagttgatg aatacggcgt cacaactgtt acattagaaa
     1021 atacgtctcc tctcgcaaaa attgattaca aaactggtat gctaatcaaa gaagatggga
     1081 aacaggcaga agaaggtgaa gatccaaata gcgatgctga tgaaaacgaa gcagctattg
     1141 aaagtgcatc tgatatagaa gaaaatacta acactaatac ttctgaatca gatacaaata
     1201 atgtagctcc ccaaaataga atcgtctacg ttgcaaataa gggtcgttca aatacttatt
     1261 ggtatagttt agaaaacata aaaaatgcaa ataccgctaa tatcgttcaa atgactgaac
     1321 aagaagcgtt aaatcaacac aaacatcaca gcactactga agcacaataa ttacaattta
     1381 ctgccggcaa aattgccggt agtatttttt atcctaaaac gcaaaaatag attcacattg
     1441 ccccttctat caacttaagc cagaaaactt agtagatacc tcatcgagat aatttctcaa
     1501 ctgatccctc ataaaatata ttcgaaaaaa acagggagat aaataaaaaa tctatcctag
     1561 aataagatag atttttttat ttaacgtttg cgaagcaagc ctactaagct aactaatagg
     1621 aaaatgccaa taaaaatcat cgttattgtt gcactagcag gtgtctccca ataatacgaa
     1681 aggaaaatac cagctaccat tcccacaaat ccaattaaca tacccagaaa aataacagtt
     1741 ttaaaatttc gacctaaccg catagcaata ctagctggta aaaccataat agttgacacc
     1801 aatagagcac ctgcggcagg aattgttaat gcaatagcaa tacctgtaac aacattgaac
     1861 aaaatagaca ttgtacgcac aggcaatcca tctacaaaag ctgtatcctc atcaaacgtt
     1921 aatatataca ttggtctaat aaataggatg gttaaaataa aagtgattaa tgcaataaca
     1981 aacaaagcaa tcacttgttc cttcccaata gtaataatag aaccaaataa atactgttca
     2041 agactgacat taccaacatt atgagcttta ctcataacga ttaaagatat agccaacccc
     2101 atcgacatca ggatagcagt tgatatttcc atataatgtt tgtatacagt acgcaaatac
     2161 tctaatacaa ctgccgccaa agtaacaacg aaaatagttg accatgtagg cgagatacct
     2221 aaaacaacgc ctaatgcaac accggctaag gaaacgtgac tcaaagtatc actcatcaaa
     2281 ctctgacgac gtaaaattag gaagatgcct aaaattggag caaaaatact aatagccaca
     2341 actgctaaga gcgctcgttg cataaaatca taggaaagca tatcaagtag catctgattc
     2401 aacctccatt tcgtttgtat ggacattgaa acaacgccat ggtaaagatt ggttcctaac
     2461 aagatgaata tttcggtcag catacccctt tacttcatca ggatcgtgag taatcatcaa
     2521 aacagactta ccatgcttat gagcattatg atgcattaac tcataaaatt tctcagtagt
     2581 ccctgcgtcc ataccagttg ttggctcatc caatacaaaa atatctggat cagaagcaaa
     2641 catcctagca atgacagccc tctgcttttg accgccagat aaacttccta tctttttatg
     2701 gcgattatcc cacataccaa cagcttctaa actaacccta atatgttctt catcatgttt
     2761 agtcagtctt ctaaaccaac catttctagg gtatcgtcca gatttcacaa attcataaac
     2821 tgacgatgga aagccagcgt taaaacttgc aatctgttga ggtaaatagg caatacgtaa
     2881 tttttttcct tctttgtttt ctttagagat attaactgta cccactttgg gcgttaaaat
     2941 acctaaagta gcctttatta aagttgattt agcagcccca ttttcacctg ttaaggtaac
     3001 aaactcccca ctgtctaaat ggtaattaac cccttccagc acaggatcgc tatcgtactg
     3061 aaaagtaaga ccactaactg taatatatcg catgattacc cttctaattc tctagagaaa
     3121 agatcaagaa aacgttctaa aacgaccttt tcgtcctttg aaaaatgatt tactaatctt
     3181 ccgtaaaccc ctaacgtatt gtcatgatga tgtgtgtgtt catctgcaat gggtttagca
     3241 agttcagata actcaaaata agtaatacga gcatctttag aatctttatt cgctttcaac
     3301 atatcctgag aaattaaact ttttactgct ttagttacag ctgcctgact aatatttaac
     3361 ttcttagcta aatcagaatt tgtcaactgc tcttgtgata aaagcatcag aatgtgttct
     3421 tgcgtattag tcaatttaac atcactttga caagtaccaa acaataattc atgttgattt
     3481 tctgctttaa gcaagatttg actcactaaa tggtctaatt tttgttctaa aactgtcata
     3541 tatacctctt ttttgttaac cagtaaatta tatcacgaag atatagaaga atcaatcata
     3601 gataggtgaa gaagataaaa ccttttatct caacaaccta actttataaa cttctttgca
     3661 aaaacctttc atactattaa aaacacgatc agcttttttc tctgtagaac acattgaaaa
     3721 aacagttggt ccacttcctg tcattaatgc aacatcggct ccagaattta acatacgttc
     3781 ttttattgta cttataactg gatttttagt aattgtaata tcctcgagtg aatttcccat
     3841 agatttgacc attaactgat aatctgatga caaaatagca gactttaata aatcaatatc
     3901 aactctactt atagacttac aatcaatatc tctaaaaatg gatttagttg aaataccaaa
     3961 atccggctta accagaacta tccaacatgg tctcaatgtc ggtaagggtt taacaatttc
     4021 acctttacct aatactaacg aacatccccc accaagacaa taaggaacat cactaccaat
     4081 tttaaaacca atagcaacca tttcgtcata gtccatttga agattccata atcgattaag
     4141 agctcttatt gtagcagcag catcagtaga accacccccc agtcctgcac agacaggaat
     4201 ggatttttct aatctaatat gaacaccttt attaatacca tattgatttt tgattatatc
     4261 tgcagcttta aacacatcat tatcattatt taaaggcatt ttgctactat cagaatcgat
     4321 aacaatacaa tcttccttta gctcagaaat ggtaacgtag tcattaagat caatactaac
     4381 cataatcata gctaattcat gataaccatc gtcacatcgt cctttaatat ctaatcctaa
     4441 attaagtttg gcaggagctt tctcaaaaat tttcataaaa cctccctaat aaaatataga
     4501 atatccatat tataacataa caaatgacaa attcggacaa ttaagatgtt agccaatctt
     4561 aaggataata attccaataa aaaaaggcta accaaagtta gtctcccttt atctactccg
     4621 ccagtaggac tcgaacctac gacatcatga ttaacagtca tgcgctacta ccaactgagc
     4681 tatggcggat tat
//
LOCUS       DATRNK010000099         4581 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.100, whole genome shotgun sequence.
ACCESSION   DATRNK010000099 DATRNK010000000
VERSION     DATRNK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.100"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..1474)
                     /locus_tag="VBC52_001936"
     CDS             complement(<1..1474)
                     /locus_tag="VBC52_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEO6883493.1"
                     /translation="MLNTKDKQPILLIIFGQKMKEYDDLIRSTVERLSKRDRPVSILI
                     SKELNHVIHHYDAQFENLETLVGYINTHEDSLIYPLCLDKNHELFQEFLVWQSHYSRQ
                     YISDNLSEKSVQAIDNNQDFVQVDCLVMRDSLNNCLYVSDLEYIESNKTTGKSLAIVP
                     SQTLSDAARQTIRDVAFDVCRKANIIGVCYFSFLIDLNSLDYHIISLSSGLSYQSILF
                     ETITTYPVLEIATKLTVGYTFSQLKHSYYPNTSAFLEPQLDYVAAVSFSFEKVDYIFF
                     ARNIEQLFLNLLEASSYDHFPFLSDISEEDLMFALIQKKENRLAYLLEAFRRGFDLYD
                     LSSVTKINPFYLDKCLHIVELYENLNKSQYNVDIYKEAKRYGFSDDYIANSWQISLID
                     MLEYRKKHSVAPVLKQVEQSSGVLTGHQIQYFRSYDWHSDYISSGCQKALIMVDKGYS
                     LVKLNELIKQIKQTRLELLIVTNQPLLIEQLNDTSIIFDTI"
     gene            complement(1530..2600)
                     /locus_tag="VBC52_001937"
     CDS             complement(1530..2600)
                     /locus_tag="VBC52_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEO6883494.1"
                     /translation="MAKKLLILEDGTVFEGLSFGSSLDVTGELVFCTGNTGYQEIITN
                     PSHNGKILVFTSPLIGNYGIHRSYSEAIIPTCLGVVVAEYSRCVSSDTSKMNLDEFLK
                     MKKVPAMSGVDTRYLMQVIKEKGFVKATLAEAGDVLSHLQDQLIATVLPTNNVEQVST
                     KTAYPSPASGRNIVVLDFGLKHSILRELSKRQCDVTVIPYNTSLEGIKNLYPEGIILS
                     NGPGNPEKLQEVLNTIKELQKSVPMLGIGLGHQLIAMANGAEIMRLPVAKKGPNYPMR
                     DIATGRLETVSQFNHFTVNRLNLPHDLLVTHEGLNDQEIVALRHRSFPVMSVQFYPEA
                     APGPHDVTYFFDEFLEMIGSRA"
     gene            complement(2649..3170)
                     /gene="pyrR"
                     /locus_tag="VBC52_001938"
     CDS             complement(2649..3170)
                     /gene="pyrR"
                     /locus_tag="VBC52_001938"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEO6883495.1"
                     /translation="MKRKEIIDDVTMKRAITRITYEIIERNKNLDNIVLAGIKTRGVF
                     LAKRIQERLKQLENLDIPVGELDTKPFRDDMKVEVDTTTMPVDITDKDIILIDDVLYT
                     GRTIRAAIDNLVSLGRPSRVSLAVLIDRGHRELPIRADYVGKNIPTSQFEEILVEVME
                     HDGYDRVSIIDPS"
     gene            complement(3346..4236)
                     /locus_tag="VBC52_001939"
     CDS             complement(3346..4236)
                     /locus_tag="VBC52_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6883496.1"
                     /translation="MEITIKIAGVRLDKALADNSELSRSQANEEIKKGIVLVNGQVKK
                     AKYTVQEGDRITFDIPKEEVLDYQAENIPLDIIYQDDDVAVVNKPQGMVVHPSAGHSS
                     GTLVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKNDRAHQVLAEELKDKKS
                     LRKYLAIVHGNLPNDRGVIEAPIGRSDKDRKKQAVTAKGKPAITRFHVLERFGDYTLV
                     ELSLETGRTHQIRVHMAYIGHPLAGDPVYGPRKTLGGKGQFLHAQTLGFTHPSNGENL
                     IFSVEVPEIFQTTLDKLRKN"
     gene            complement(4220..>4581)
                     /locus_tag="VBC52_001940"
     CDS             complement(4220..>4581)
                     /locus_tag="VBC52_001940"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEO6883497.1"
                     /translation="FIPNIVSLTYLRNYGAAFSILQNQQWLFTLITIFVVCVAIIYLM
                     KHINGSYWLLISLTLIISGGLGNFIDRLRLGYVVDMVHLDFINFAIFNVADSYLTIGI
                     ICLMIALWKEESNGNHN"
BASE COUNT         1511 a          908 c          691 g         1471 t
ORIGIN      
        1 ctatagtgtc aaaaattata gatgtatcat ttaattgttc tatgaggaga ggttgattgg
       61 taacaattaa taattctaaa cgagtttgtt tgatttgctt aattaactca tttaatttta
      121 ctagggaata acccttgtct accattatca aagccttttg acaaccgcta gaaatgtagt
      181 ctgaatgcca atcatagctt cgaaaatact gaatttgatg gccagttaga acaccagaac
      241 tttgttcgac ttgctttaga acaggtgcaa cactatgttt ttttcgatat tctaacatgt
      301 ctatcaaaga gatttgccaa ctattagcaa tatagtcatc cgaaaaacca taccttttag
      361 cttccttgta aatatctaca ttatattggc ttttattcag gttttcatac aactctacaa
      421 tatgtaaaca tttatctaag taaaaagggt tgattttagt aactgaggat aaatcataca
      481 aatcaaagcc acgtctaaaa gcctctaaaa gataagctag acgattttct tttttctgaa
      541 tcaaagcaaa catcaaatct tcctcgctaa tatcactcag aaatggaaaa tgatcataac
      601 tgctagcctc gagtaaattt aaaaataatt gctctatatt acgagcaaaa aagatataat
      661 caactttttc aaaactaaaa gatacggcag ctacatagtc taattgtggc tctaaaaagg
      721 cagaggtatt tgggtaataa gaatgcttca gttgactaaa agtatatccc actgtcaatt
      781 tagttgctat ctctaaaaca ggataggtcg taatagtctc aaataaaatg ctttgataag
      841 acaaaccaga tgataagcta attatatgat aatccaaact gttcaaatct attaagaaac
      901 taaagtaaca tactccaata atgtttgctt ttcgacacac atcaaatgca acatctcgaa
      961 ttgtttgcct agcagcatcg gataaagttt gacttggtac aatcgctaga gacttcccag
     1021 ttgttttatt agattcaata tattctaaat cagaaacata taaacaatta ttcaaagagt
     1081 ctctcattac taaacaatct acttgcacga agtcttgatt attgtctatt gcctgaacac
     1141 ttttttctga taaattatca ctgatatact gccttgaata atgactctgc cacactaaaa
     1201 actcttgaaa tagctcatga tttttatcta agcaaagggg gtaaatcaaa ctatcttcat
     1261 gtgtatttat ataccccaca agcgtctcaa gattttcaaa ttgtgcatca taatggtgaa
     1321 tcacatggtt tagctccttc gagataagaa tactaacagg tctatccctt ttagataacc
     1381 tctcaacagt tgatctaatt aaatcgtcat attctttcat tttctgtccg aaaataataa
     1441 gcaaaatagg ctgtttatcc tttgtattta gcatatacgc acctccttat taatcagctc
     1501 accgactgac tttaaataat atcttatgtt taagcgcgtg atccaatcat ttctaaaaat
     1561 tcatcaaaaa aataggtcac atcatgaggg cctggagcag cctctgggta aaattgcaca
     1621 gacatcactg gaaatgagcg gtgccttaga gcaacaatct cttgatcatt caaaccttcg
     1681 tgtgttacca gtaaatcatg cggaagattt agccggttaa cggtaaaatg attaaactga
     1741 ctaactgttt ctaaacgacc cgtagcaata tctctcatgg gataatttgg ccccttctta
     1801 gcaactggga gtctcataat ttctgcacca tttgccatgg caattaattg atgccccaaa
     1861 ccaatgccta acataggaac agatttttgt aattctttaa tagtgtttaa gacttcctga
     1921 agcttttctg gattccccgg accattagat aatatgatac cttctgggta tagattttta
     1981 ataccttcta aagacgtatt gtagggaatg acagtcacgt cacactgacg tttagacaat
     2041 tctctcaaaa tagaatgttt aaggccaaaa tcaagtacaa caatatttcg gccgctagca
     2101 ggcgacggat aagctgtttt tgtcgaaact tgttccacat tatttgtcgg taatactgtc
     2161 gctattaatt gatcttgtaa atgtgataaa acatcacctg cttctgctaa agtagctttt
     2221 acaaatcctt tttctttaat aacttgcatc aaataccttg tatcgacccc cgacattgca
     2281 ggaacctttt tcattttcaa aaattcatct agattcatct tgctagtatc gctacttaca
     2341 catctggaat actctgcaac aacaactcct aaacaagtgg gaataatggc ttcagagtaa
     2401 ctgcgatgaa taccataatt tcctattagc ggtgaggtaa aaactaaaat ttttccatta
     2461 tgtgaaggat tcgttattat ttcctgatag ccggtattcc ccgtacaaaa aactaattcg
     2521 ccagttacat caagagatga accaaaggat agtccttcaa aaaccgtacc atcctctaga
     2581 attaacaatt tttttgccat tgtggttctt ccccttctat aaaatcatcg taaataatag
     2641 tcgtctactt aactcggatc aataatactc acacgatcgt aaccatcatg ttccatcact
     2701 tcaactagaa tttcttcaaa ttgacttgtt ggaatatttt tccccacata atctgcacga
     2761 atgggtagtt cacgatgccc acggtcaatt aatacagcta aactgacacg actcggacgc
     2821 cccaaactaa caagattatc aatagcagct cgaatagtgc gacctgtata gagaacatca
     2881 tcaattaata taatatcttt atcagtgatg tcaactggca tagttgtggt gtcaacttca
     2941 actttcatat catcacggaa aggtttagta tccaattctc caacgggaat atctaaattt
     3001 tctaattgct tcaagcgttc ttgaatgcgt ttggctaaaa aaacacctct tgttttaatg
     3061 cctgctaaga caatattatc taaatttttg ttacgttcta taatttcgta ggtaatacgt
     3121 gtaatagcac gtttcatggt cacatcatca ataatttctt ttcttttcat tgcagtatcc
     3181 tctctaagat aaaagagccc cttcaaaagg agactctcta gaatgacaaa gccgctcagg
     3241 cattgattta gtctgaagtt tctccttaac tatctcactg gataaggtta aaggaatgat
     3301 ttgcttatat tatagcaaat ggtaaaagta tttttcaaga aaaacttaat tttttctcaa
     3361 cttgtctaag gttgtctgaa aaatttccgg aacctctact gaaaaaatca aattctctcc
     3421 gttacttggg tgcgtaaaac ctagtgtttg tgcatgcaaa aactgtcctt taccccctaa
     3481 agtcttacgg ggaccgtaga caggatcacc ggctagagga tggccaatat aagccatatg
     3541 aacccgaatt tggtgtgttc gacctgtctc caatgacaat tcaactaagg tataatcacc
     3601 aaaacgttct aaaacatgaa aacgagttat tgctggctta cctttagcag tcactgcttg
     3661 cttcttacga tctttatcgc ttctaccaat cggagcctca atcacacctc gatcatttgg
     3721 taaattacca tgcacaattg ctaaatattt acgaagcgat tttttatctt ttaactcttc
     3781 agccaacact tggtgagctc tatcattttt tgcaaccatc aataaaccag aagtatcttt
     3841 atcaatacga tgaacgatcc ctgggcgaac aacgccatta attgatgata aatccttaat
     3901 atgatacatc aaggcgttaa ctaaggtgcc cgaactgtgg cctgcagaag ggtgaacaac
     3961 cattccttgc ggcttattaa ccacagcaac gtcatcatct tgatagataa tatctaaagg
     4021 gatattctca gcttgataat ctagtacttc ctctttagga atatcaaaag taatccgatc
     4081 tccttcttga acagtatatt tcgctttttt tacttgtcca tttactaaca caatcccttt
     4141 tttaatttct tcattagctt gactacgcga cagttcagaa ttatcagcta aagctttatc
     4201 tagcctaact cctgctattt taattgtgat ttccattact ttcctctttc caaagtgcaa
     4261 tcattaagca aataatacca attgtcagat atgagtctgc tacattaaag atagcaaaat
     4321 taataaaatc taaatgcacc atatcaacga cataacccaa tcttaagcga tctataaagt
     4381 ttcctaatcc accagagatg attaaggtta gagaaattaa taaccaataa cttccattaa
     4441 tatgtttcat caaataaata atagcaacac aaacaacaaa gattgtaatt aaggtaaaaa
     4501 gccattgttg attttgcaaa atagaaaatg cagcaccata atttctcaga taagttagac
     4561 taacgatatt tggaatgaat t
//
LOCUS       DATRNK010000100         4541 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.101, whole genome shotgun sequence.
ACCESSION   DATRNK010000100 DATRNK010000000
VERSION     DATRNK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.101"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            60..284
                     /locus_tag="VBC52_001941"
     CDS             60..284
                     /locus_tag="VBC52_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEO6883498.1"
                     /translation="MAAITVFSKNNCMQCKMTKKFLDQHGADFEEINIDEKPEKIEYV
                     KNLGFSAAPVIEAGNVVFSGFQPSKLKELV"
     gene            362..2521
                     /gene="nrdE"
                     /locus_tag="VBC52_001942"
     CDS             362..2521
                     /gene="nrdE"
                     /locus_tag="VBC52_001942"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO6883499.1"
                     /translation="MSLKNIGDVSYFRLNNEINRPVNGQIPLHKDKEALTAFFKENVQ
                     PNSKAFDSITDKIAYLLKYDYLEEAFLNKYRPEFIEELSTKLFDKKFRFKSFMAAYKF
                     YQQYALKTNDGEYYLESIEDRVLFNALYFADGDEELATDLALEMISQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNAIGRSINSALQLSRIGGGVGISLSNLREAGAPIKGF
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLDVFHPDIISFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARNNQEMYLFSPYSIEREYGVPFSYIDITEKYDELVANPNI
                     TKTKINARDLETEISKLQQESGYPYIINIDTANRTNPVDGKIIMSNLCSEILQVQKPS
                     LINDAQEYLEMGTDISCNLGSTNVLNMMTSPDFGKSIKTMTRALTFVTDSSNIEAVPT
                     IKNGNAQAHTFGLGAMGLHSYLAKNHIEYGSPESIEFTDIYFMLMNYWTLVESNNIAR
                     ERQTTFVGFEKSKYADGTYFDKYVSGKFVPQSDKVKSLFANHFIPEAKDWENLRYAVM
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLATDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSMTLFLRSELPKELYEWKTESKQTTRDLS
                     ILRNYAFNKGVKSIYYIRTFTDDGSEVGANQCESCVI"
     gene            2724..3683
                     /gene="nrdF"
                     /locus_tag="VBC52_001943"
     CDS             2724..3683
                     /gene="nrdF"
                     /locus_tag="VBC52_001943"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO6883500.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSAQEKDLVGKVFGGLTLLDTMQSETGIEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKSEIEEIFEWTNNNEFLQEKARIINDIYANGNALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMHDDDYNYGL"
     regulatory      3768..3866
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3924..4487
                     /gene="thiT"
                     /locus_tag="VBC52_001944"
     CDS             3924..4487
                     /gene="thiT"
                     /locus_tag="VBC52_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEO6883501.1"
                     /translation="MSKNNNTTCLIETAIFAALAMALSMIPDFASWFTPSFGAIPLIL
                     FALRRGTKYGLFAGLIWGLLHFVLSKVYYLSLSQVFIEYILAFISMGVAGVFSAKFKD
                     ALSSSSKTKALSLALSGAILATFVRYVWHYIAGVIFWASYAPKGMSATLYSLSVNGTA
                     GLLTLFFVVISIIILVISYPSFFLPKK"
BASE COUNT         1489 a          809 c          762 g         1481 t
ORIGIN      
        1 cttgcttttt ccattctttt tttatatgct attaccatga aaaaaattag gaggacaaca
       61 tggcagctat tactgttttt tcgaaaaaca actgtatgca atgtaaaatg actaagaaat
      121 ttcttgacca gcacggcgct gattttgaag aaattaatat tgatgaaaaa ccagaaaaaa
      181 ttgaatatgt gaaaaatctc ggcttttctg cggctccagt gattgaagct ggtaatgttg
      241 ttttttcagg tttccaacca agcaaactaa aagaacttgt ttaagcaaat tagagtctgg
      301 gaataaagca attaattgct ttattcccat tttgtattgt taagttaaga ggataaattc
      361 aatgagttta aaaaatattg gcgatgtatc ttactttcgc ctaaataatg aaattaaccg
      421 tcctgttaat ggacaaatcc cactccataa agataaagaa gccttaactg cttttttcaa
      481 agaaaacgta cagcctaatt caaaagcttt tgactcaatc actgataaaa ttgcttacct
      541 ccttaagtat gattatcttg aagaagcttt cctcaataaa tatcgtcctg aatttattga
      601 agaattatct accaaactct tcgataaaaa gtttcgtttt aaatccttca tggcagccta
      661 taagttttat cagcagtatg cattaaaaac aaatgatgga gaatactatt tagaaagtat
      721 tgaagatcgc gttctcttta acgctcttta ttttgcagac ggtgatgaag aactagcaac
      781 tgacttagct ctagaaatga tttcacaacg ctatcaacct gcaacaccaa gttttttaaa
      841 tgctggtcga agtcgtcgcg gagaattagt ctcatgtttc ttaatccaag taactgatga
      901 tatgaatgct attggtcgtt cgattaattc agctcttcaa ttatctcgta ttggtggtgg
      961 ggttggaata tcacttagta accttcgtga ggctggagct cctatcaaag gttttgctgg
     1021 tgctgcttct ggagtcgttc cagtcatgaa attatttgaa gacagttttt cttactccaa
     1081 ccaacttgga caacgccaag gtgctggagt tgtttacctt gacgttttcc atccagatat
     1141 tatttcattc ttatcaacta aaaaagaaaa tgctgatgaa aaagtacgtg ttaaaacatt
     1201 gtcattaggg attactgtac ccgataaatt ctatgaacta gcacgtaaca atcaagaaat
     1261 gtacttgttt agtccttata gtatcgaacg tgaatatggt gttcctttca gctatattga
     1321 tattaccgaa aaatacgatg aattagtcgc taaccctaat atcactaaaa caaaaatcaa
     1381 tgctcgtgat ttagaaacag agatttctaa acttcaacaa gaatcaggtt atccttacat
     1441 catcaatatt gacactgcta atcgtactaa ccctgttgat ggaaaaatta tcatgtcaaa
     1501 tctttgctct gagattttgc aagtacaaaa accaagtctt atcaatgatg ctcaagaata
     1561 ccttgaaatg ggaactgata tttcttgtaa ccttggctca actaatgttt taaatatgat
     1621 gacatctcct gattttggaa aatcaattaa aacaatgact cgtgctttaa ctttcgttac
     1681 tgactcatca aatattgaag ctgttccaac tattaaaaac ggaaatgctc aagcacatac
     1741 atttggactt ggagcaatgg gactccactc ctatttggca aaaaatcata tcgaatatgg
     1801 tagtccagaa tcaattgaat ttactgatat ttatttcatg ctaatgaact actggacctt
     1861 agtagaatca aacaatattg cccgcgaacg tcaaacaaca tttgtaggtt ttgaaaaatc
     1921 aaaatacgct gacggtacat actttgataa atatgtatct ggaaaatttg ttccacaatc
     1981 tgacaaagta aaatccctat ttgctaatca ttttatcccc gaagctaaag attgggaaaa
     2041 ccttagatac gcagttatga aagacggact atatcatcaa aatcggcttg cagttgctcc
     2101 gaatggttct atttcgtata tcaacgattg ttcagcatct atccacccta ttacacaacg
     2161 tattgaagag cgtcaagaga aaaaaatcgg gaaaatttat tatccagcta atggcttagc
     2221 tacagatact atcccttatt acacttctgc ctatgatatg gatatgcgta aagtaataga
     2281 cgtctatgcc gcagcaactg agcacgtaga ccaaggtctc tcaatgacac tctttttacg
     2341 aagcgaatta ccaaaggagt tgtacgagtg gaaaacagaa agtaagcaga ctactcgcga
     2401 cctttcaatc ctacgcaact atgctttcaa caaaggtgtg aaatcaatct actatatccg
     2461 tacttttaca gatgatggta gcgaagtcgg agctaatcaa tgcgaaagtt gtgtcatcta
     2521 acgaagaaac tctacttaaa acatcagaaa ctagactgag ataaactatc tcagtttcat
     2581 gatgttatct tattttctaa ggtattttca tctttatttt tctaaaaatc gataatactt
     2641 gaaatattgc tagatcagaa ttctatattc tactcttctt ccatcatcat tatataaaaa
     2701 cctatttacc attggagaaa accatgacaa cttactacga agctataaac tggaacgaaa
     2761 ttgaagatgt tattgataaa tcaacttggg aaaaactaac cgaacaattt tggctcgata
     2821 cacgtatccc tttatcaaat gacttagacg attggcgcaa actttctgct caagaaaaag
     2881 atcttgttgg caaggttttt ggaggcttaa ccctacttga taccatgcaa tcagaaactg
     2941 gtattgaagc tattcgtgcc gatgttcgca cgcctcacga agaagctgtc ttaaacaata
     3001 ttcaattcat ggaatctgtt cacgctaaat cttattcttc aattttctca actttaaata
     3061 ctaaatcaga aattgaagaa attttcgagt ggactaataa taatgagttc cttcaagaaa
     3121 aagcacgtat tatcaatgac atttatgcta atggaaatgc ccttcaaaaa aaggtggctt
     3181 ccacctacct cgaaactttc cttttttatt ctggcttttt cacacctctt tactatttgg
     3241 gaaataataa gttagcaaat gttgctgaaa tcattaaatt aattattcgt gatgaatctg
     3301 tacatggtac ttatatcggt tacaaattcc agcttggttt taacgaatta ccagaagatg
     3361 agcaagagaa ttttcgtgat tggatgtatg acctccttta tcagctgtat gaaaacgaag
     3421 aaaaatacac caagacactt tatgatggcg taggatggac tgaagaagtt atgacctttt
     3481 tacgctacaa tgctaataaa gctcttatga atttaggaca agatccttta ttcccagata
     3541 cagcaaatga tgtcaaccca attgttatga atggtatttc aacaggaaca tcaaaccatg
     3601 acttcttctc tcaagtaggt aatggttacc tacttggtag cgttgaagct atgcatgatg
     3661 atgactataa ctatggatta taagaaaaag acactgattt agtgtctttt tctagttaaa
     3721 tacaaatttc ttgacaagca aaccctttca agatagaatg ttagagtaaa tacacaaggg
     3781 agtgccttga gctgagattg cagatatgca aaatcctcta acctgatctc gttaggacga
     3841 gcgtaggaat tgtggtttga attggttaaa catgacaaat gacaaaagaa tctcctttgc
     3901 tgtataaaaa aaggagattt tttatgtcaa aaaataacaa cactacctgt cttattgaaa
     3961 ctgctatttt tgcagcgctt gctatggctt tatcaatgat tcctgatttt gcgtcatggt
     4021 tcactccctc ttttggagca attcctctga tactttttgc attacgtcgt ggtacaaaat
     4081 acggtctatt tgctggctta atttggggct tattgcactt tgttctttcc aaagtatatt
     4141 accttagttt atcccaagtt tttatagaat acatcttagc atttataagt atgggagttg
     4201 caggcgtatt ttctgctaaa tttaaagatg cacttagcag cagctctaaa actaaagctt
     4261 tatcattagc attatctgga gcaatattag caacatttgt tcgttatgtt tggcattata
     4321 tcgctggtgt tattttttgg gctagttatg caccaaaagg tatgtctgct acattatact
     4381 cattaagcgt caatggtact gctggtttgt taactctatt ttttgtagtc attagtatta
     4441 ttattttagt tatcagctac cctagcttct ttttacctaa gaaatagaaa aaataaaagg
     4501 actgattttc agagaatcaa tccttttatt ttacactaat a
//
LOCUS       DATRNK010000101         4154 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.102, whole genome shotgun sequence.
ACCESSION   DATRNK010000101 DATRNK010000000
VERSION     DATRNK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.102"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            73..1287
                     /locus_tag="VBC52_001945"
     CDS             73..1287
                     /locus_tag="VBC52_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO6883502.1"
                     /translation="MANASLRHQLFEKLDQKCDQMVAIRRYLHENPELSFKETKTAAY
                     ISDFYKGKDCHVQTQFGGMNGVVVDIYGDKATDKPIKHIALRADFDALPIQEETGLSF
                     ASKTAGVMHACGHDAHTAYLLILAESLIELKSEFSGHIRILHQPAEEVPPGGAKAMIE
                     AGCLDGIDAVLGIHVMSTMEEGTVQYHAGPIQTGRATFKVILQGKGGHGSMPHRANDT
                     IVAASSFVMAAQTIVSRRVNPFDTAVVTIGSFDGKGSANVIKDSVTLEGDVRVMSEET
                     RGVVEEEFKRILEGIAQTYGVSYQLDYQNDYPVLVNNSEVTQKVANSLKSVAIKEILD
                     VIDCDPQTPSEDFAYYAQTIPACFFYVGAHEEGQPYYPHHHPKFQIAESSLMVSAKSM
                     ATAALAMLEEGE"
     gene            1295..2677
                     /locus_tag="VBC52_001946"
     CDS             1295..2677
                     /locus_tag="VBC52_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6883503.1"
                     /translation="MLTTKENFKGNNKLLVGIVLAVLSFWLFAQSILNMGPDVQSSLG
                     ISSGAMDIGVSSTALFSGLFIVVTGGLADKLGRVKFTFIGLCLNIIGSLLIVLANGAV
                     LFIMGRIFQGLAAAFIMPSTMALVKTYYDGKDRQRAVSFWSIGSWGGSGLCSYFGGAV
                     ASTLGWRYVFIFSIIASVVSFLLILGTPESKNVGQKTHFDYLGLIIFIISMLSLNIGI
                     SMAQEHGLMNVIPLSLFTVMLIGFVLFYYVETRKSNSFIDFHLFENRFYLGATISNFL
                     LNAVAGTLIVINTYMQQGRQLTPKVTGEMSLGYLVCVLIAIRVGEKILQRFGARKPML
                     LGAMSTFVGIFLMTLVNIQGPLYLVLVFVGYALFGTGLGIYATPSTDTAISSIPNEKV
                     GSASGIYKMASSLGGAIGVATSIAIYHAFSGNADFHKAALCGLILNLVFCSLSILSIL
                     FVIPKKIEDK"
     gene            2888..3313
                     /gene="rplK"
                     /locus_tag="VBC52_001947"
     CDS             2888..3313
                     /gene="rplK"
                     /locus_tag="VBC52_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEO6883504.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGEPNKTKVATVTR
                     AQVQEIAETKMPDLNAANLESAMRMIEGTARSMGFTVTD"
     gene            3417..4106
                     /gene="rplA"
                     /locus_tag="VBC52_001948"
     CDS             3417..4106
                     /gene="rplA"
                     /locus_tag="VBC52_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEO6883505.1"
                     /translation="MAKKSKNLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     SYNLNIDVKKADQQIRGAMVLPAGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KIQGGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDDAKLVDNFKAFNDVIVKAKPATAKGTYITNLSITTTQ
                     GVGIKVDPNSL"
BASE COUNT         1286 a          679 c          852 g         1337 t
ORIGIN      
        1 tagactatat aagagcagat tgttagaata atagctttat tttaactgtt catattttaa
       61 ggaggaaagc aaatggccaa tgcatctttg cgacatcaat tatttgaaaa acttgaccaa
      121 aaatgtgatc aaatggttgc tattcgtcgc tatttacatg aaaatcccga attatcattt
      181 aaagagacaa aaacagcagc ttatatttca gatttttata aaggtaagga ctgtcatgtt
      241 cagacacaat ttggtggtat gaatggcgtc gtagtcgata tctatggtga taaagcgaca
      301 gataagccca tcaaacacat tgctttgaga gccgactttg atgctcttcc aattcaggaa
      361 gagacagggc tttcttttgc ttcaaaaaca gcgggcgtca tgcatgcttg tggacatgat
      421 gcccatacag cttatttatt gattttggct gagagcttga ttgaattaaa atcagaattt
      481 tcaggtcata tccgtatctt acatcaaccg gctgaagagg taccaccggg cggagcaaaa
      541 gcaatgattg aagcaggatg tttagatggc attgatgctg tccttggtat ccatgttatg
      601 tcaacgatgg aagaggggac ggtacaatat cacgcaggtc ccattcaaac agggcgtgcc
      661 acttttaaag ttattttgca aggtaagggt ggacatggtt ccatgccaca tcgtgcaaat
      721 gatacaattg ttgcagcgtc tagttttgtt atggcagcgc aaacgattgt cagtcgtcga
      781 gttaatccat ttgacacagc ggtagttact atcggatctt ttgatggtaa aggttccgct
      841 aatgtgatca aagatagtgt aaccttagaa ggtgatgtgc gcgtgatgtc tgaagagaca
      901 cgaggtgttg ttgaggagga atttaagcgt attctagagg gaatagcaca aacttatggt
      961 gttagttatc agttagacta tcagaatgat tatcctgttt tagttaataa cagtgaagtg
     1021 acacagaaag ttgcaaatag tttaaaatct gttgctataa aagaaatttt agacgtcatt
     1081 gattgcgatc ctcaaacgcc gtctgaagat tttgcctatt atgctcaaac aatcccagct
     1141 tgcttcttct atgttggagc acacgaagag gggcaaccat attacccaca tcatcatcca
     1201 aaattccaaa ttgcagaatc atcattgatg gtatctgcta aatcaatggc aactgcagcg
     1261 cttgctatgc ttgaagaagg agaatagata acatatgtta acgacaaagg aaaatttcaa
     1321 aggaaataac aaattattgg tgggaattgt actcgctgta ctatcatttt ggttatttgc
     1381 acagtctatt ttaaacatgg gaccagatgt tcagtcatca ttaggtattt catctggtgc
     1441 aatggatatt ggtgtctcaa gtacggcatt gttttcaggt ttatttattg ttgtaactgg
     1501 tggtttagca gataaacttg gtcgagttaa atttactttt attggtcttt gcttaaatat
     1561 tattggttct ctactaattg ttttagctaa tggagcagtg ctatttatta tgggacgtat
     1621 ttttcaagga ttggcagcag cttttattat gccatcaaca atggccttgg ttaaaacgta
     1681 ctacgatggt aaagatagac aacgtgcagt tagtttctgg tcaatcggat catggggtgg
     1741 ctcaggtcta tgttcttatt ttggtggagc ggtggcaagt actctaggtt ggcgttatgt
     1801 ctttatcttt tcaatcatcg catcagtagt tagttttcta ttaattttag gaaccccaga
     1861 aagtaaaaat gttggacaaa aaactcactt tgattacctt ggtttaatta tttttattat
     1921 ttcaatgtta tcgttaaata ttgggatatc tatggctcaa gaacatggat tgatgaacgt
     1981 tattccattg agtcttttta cagtaatgct tattggattt gttctgtttt attatgttga
     2041 aacacgtaaa tctaatagtt ttattgactt tcatctgttt gaaaatcgat tttacttagg
     2101 cgcaacaata tcaaatttct tattgaatgc agttgcagga actctgattg ttattaatac
     2161 ttacatgcaa caaggtcgcc agctaacacc aaaggttaca ggtgaaatgt ctttaggcta
     2221 tttggtttgt gtactaattg ctatccgagt tggtgagaaa attttacaac gctttggtgc
     2281 ccgtaagcca atgcttttgg gagctatgag tacatttgtt gggatatttt taatgacttt
     2341 ggtcaatatt caaggccctt tgtatcttgt gttagtcttt gttggttatg ctttatttgg
     2401 gacaggcttg gggatctatg cgacaccatc aacggataca gctatttcta gtattcctaa
     2461 tgaaaaagta ggttctgcat ctggaattta caaaatggca agttcactag gtggtgcaat
     2521 tggtgtagca acttcaattg cgatttatca tgctttttct ggtaatgcag attttcacaa
     2581 agcagctttg tgtggattaa ttttaaacct agttttttgt agtctatcga tcttatcaat
     2641 tctttttgtt atccctaaga aaattgagga taaataaata acaatacttg agattcaaaa
     2701 tctcaagtat tgttgtaata atgtaaaagg aattgggaaa gaaatttaaa atataatgaa
     2761 attaatggct taaaactatt gcttttattt atgattatag tataatggta aggtatgtta
     2821 taaacatacc tgtgggaggt aaaaatttct aattaccgcc aaaaccacaa caggaggatt
     2881 tttaaaaatg gctaaaaaag ttgaaaaact tgtaaaactt caaattcctg ctggtaaagc
     2941 aactccagct ccaccagttg gaccagcact tggtcaagca ggaatcaaca tcatgggatt
     3001 cactaaagag tttaacgctc gtacagctga tcaagctggt atgattattc cagttgttat
     3061 ctcagtatat gaagacaaat catttgattt catcactaaa acaccaccag ctgctgttct
     3121 tttgaaaaaa gctgcaggtg ttgaaaaagg atcaggtgaa cctaacaaaa ctaaagttgc
     3181 aacagttact cgtgcgcaag tacaagaaat tgctgaaact aaaatgccag atttaaacgc
     3241 tgcaaacctt gagtctgcaa tgcgtatgat cgaaggtact gctcgttcta tgggattcac
     3301 tgttactgac taatcagaaa cacccacata acccgcaaga cttcatcgta gtttgatgag
     3361 tgacgtggga gattttaaat caaatcgatt gaccacatta caaggagaat tttaaaatgg
     3421 ctaaaaaaag taaaaactta cgtgctgctc ttgagaaaat tgacagcact aaagcataca
     3481 gcgtagaaga agctgtagct cttgcaaaag aaactaactt tgctaaattt gatgcaactg
     3541 ttgaagtttc ttataatctt aacattgacg ttaaaaaagc tgaccaacaa atccgtggtg
     3601 ctatggtttt accagcaggt actggtaaaa cttcacgtgt tcttgttttt gctcgtggcg
     3661 ctaaagctga agaagctaaa gcagcaggtg cagactttgt cggtgaagat gacttagttg
     3721 ctaaaatcca aggtggatgg cttgactttg atgttgttat cgcaacacca gatatgatgg
     3781 cacttgttgg acgtcttgga cgtgtccttg gacctcgtaa cttgatgcca aatcctaaaa
     3841 ctggtacagt aactatggac gttgctaaag cagttgaaga gtctaaaggt ggtaaaatta
     3901 cttaccgtgc tgataaagca ggtaacgtcc aagcccttat tggtaaagtt tcatttgatg
     3961 atgctaaatt agttgataac ttcaaagcat ttaatgatgt tattgttaaa gctaaaccag
     4021 ctactgcaaa aggaacttac atcacaaacc tttcaattac aacaacacaa ggtgttggta
     4081 tcaaagttga tcctaactca ctttaatcaa taaataaaac taggaattcc ttcctagttt
     4141 tatttattct atat
//
LOCUS       DATRNK010000102         3960 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.103, whole genome shotgun sequence.
ACCESSION   DATRNK010000102 DATRNK010000000
VERSION     DATRNK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.103"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(1..1209)
                     /locus_tag="VBC52_001949"
                     /pseudo
     CDS             complement(1..1209)
                     /locus_tag="VBC52_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771531.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1491..>3960
                     /gene="pilA"
                     /locus_tag="VBC52_001950"
     CDS             1491..>3960
                     /gene="pilA"
                     /locus_tag="VBC52_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2a pilus adhesin PilA"
                     /protein_id="HEO6883506.1"
                     /translation="MRKYQKFSKILTLSLFCLSQIPLNTNVLGESTVPENGAKGKLVV
                     KKTDDQNKPLSKATFVLKTTAHPESKIEKVTAELTGEATFDNLIPGDYTLSEETAPEG
                     YKKTNQTWQVKVESNGKTTIQNSGDKNSTIGQNQEELDKQYPPTGIYEDTKESYKLEH
                     VKGSVPNGKSEAKAVNPYSSEGEHIREIPEGTLSKRISEVGDLAHNKYKIELTVSGKT
                     IVKPVDKQKPLDVVFVLDNSNSMNNDGPNFQRHNKAKKAAEALGTAVKDILGANSDNR
                     VALVTYGSDIFDGRSVDVVKGFKEDDKYYGLQTKFTIQTENYSHKQLTNNAEEIIKRI
                     PTEAPKAKWGSTTNGLTPEQQKEYYLSKVGETFTMKAFMEADDILSQVNRNSQKIIVH
                     VTDGVPTRSYAINNFKLGASYESQFEQMKKNGYLNKSNFLLTDKPEDIKGNGESYFLF
                     PLDSYQTQIISGNLQKLHYLDLNLNYPKGTFYRNGPVREHGTPTKLYINSLKQKNYDI
                     FNFGIDISGFRQVYNEDYKKNQDGTFQKLKEEAFELSDGEITELMKSFSSKPEYYTPI
                     VTSSDASNNEILSKIQQQFEKILTKENSIVNGTIEDPMGDKINLQLGNGQTLQPSDYT
                     LQGNDGSIMKDSIATGGPNNDGGILKGVKLEYIKNKLYVRGLNLGEGQKVTLTYDVKL
                     DDSFISNKFYDTNGRTTLNPKSEDPNTLRDFPIPKIRDVREYPTITIKNEKKLGEIEF
                     TKVDKDNNKLLLKGATFELQEFNEDYKLYLPIKNNNSKVVTGENGKISYKDLKDGKYQ
                     LIEAVSPKDYQKITNKPILTFEVVK"
BASE COUNT         1560 a          517 c          698 g         1185 t
ORIGIN      
        1 taataaagtc tgtagttagt acggctatct gtatcggagg aatatgattt ttgatgattc
       61 tttctagatg agtacaaagg agaagaaaat gttgttcttt aaacgtgtat tctccaattt
      121 tacttaacca atttgtaatt atcggtcgga gaatagtagt taatttatca tgtctatgat
      181 agttccatga aggaaatgaa attgtttcag ggattaattg atttaatcct aaaataaatg
      241 ttcggaaaaa aagatagcaa cttttaccaa ctcatcatga taggagccag ataagggaag
      301 agcatctttg agtggctgta ataatttttg gaatttggga taattttcga aaatagctat
      361 tacattatcg atacgcttct cagtccaatt tggattagaa aaagagttat gggaagtaag
      421 gtaagcaaga aaaatatagt ctatatcgtc tttttttaaa ttaattttaa aggaatctat
      481 aataacattt ttgacacaat attgtaatgt atcatagata aatatatttt gtagttcagt
      541 gaaaagggat gagtaaggga caactacatt ataattaata cgtttccatg atagagacaa
      601 caatgtatca aagaaaataa atgtatttgg gaagtaatga gaagcagtaa aagtagttga
      661 gtgttctaat aacaatcgtt tgatgagcaa tttatccatc ttagacatgg gataaacttc
      721 tataccaaat tgagcattta aaaatgcgat taaatagcga attcgatact cgtcaccggt
      781 aatagtattt ttgacaatct gaaatccaag tttttctagt aatggactaa tatgttttcg
      841 tatgcgataa gcagttgcac aagatagtcc aaatttttca ctaaagtaag ttaaaggtaa
      901 cctgcctgaa tcattcgtga tcaaaaattt taaaagttgt aaaacgtgtg attgatgata
      961 aatatatttg aaagcttgtt ctctagttac accattagat tggtagacta ttgtactctt
     1021 ttcgatattc agagagagtt gactgttaaa taaagttgtt aaatcatgac aatatttcct
     1081 aataagtagt tcagataaac caagttgatg acatgatttg tctagagtaa tggaagggta
     1141 gtaatcatag cataaagtta gaagttttat ttgattaagg atgtcttttt ctaaataatt
     1201 ttcaatcaat tcagtaaact ctctcaaaca ttatttttaa gcaatatttt aaaacataaa
     1261 aaaagaaaaa tcaactactt aagctcattg aaatatttct aagataataa aaaataagat
     1321 tatcaaataa aaagaaaaat tattcaaaaa ttgggaaaaa acattaaaat tccatacctt
     1381 ataataagaa attattgata tcataataag tgatagtttg tatattctag gatattctgt
     1441 atctgatctt agatttagaa acgacatttc ggcacaatag gagttgtaaa atgagaaaat
     1501 accaaaaatt ttctaaaata ttgacgttaa gtcttttttg tttgtcgcaa ataccgctta
     1561 ataccaatgt tttaggggaa agtaccgtac cggaaaatgg tgctaaagga aagttagttg
     1621 ttaaaaagac agatgaccag aacaaaccac tttcaaaagc tacctttgtt ttaaaaacta
     1681 ctgctcatcc agaaagtaaa atagaaaaag taactgctga gctaacaggt gaagctactt
     1741 ttgataatct catacctgga gattatactt tatcagaaga aacagcgccc gaaggttata
     1801 aaaagactaa ccagacttgg caagttaagg ttgagagtaa tggaaaaact acgatacaaa
     1861 atagtggtga taaaaattcc acaattggac aaaatcagga agaactagat aagcagtatc
     1921 cccccacagg aatttatgaa gatacaaagg aatcttataa acttgagcat gttaaaggtt
     1981 cagttccaaa tggaaagtca gaggcaaaag cagttaaccc atattcaagt gaaggtgagc
     2041 atataagaga aattccagag ggaacattat ctaaacgtat ttcagaagta ggtgatttag
     2101 ctcataataa atataaaatt gagttaactg tcagtggaaa aaccatagta aaaccagtgg
     2161 acaaacaaaa gccgttagat gttgtcttcg tactcgataa ttctaactca atgaataacg
     2221 atggcccaaa ttttcaaagg cataataaag ccaagaaagc tgccgaagct cttgggaccg
     2281 cagtaaaaga tattttagga gcaaacagtg ataatagggt tgcattagtt acctatggtt
     2341 cagatatttt tgatggtagg agtgtagatg tcgtaaaagg atttaaagaa gatgataaat
     2401 attatggcct tcaaactaag ttcacaattc agacagagaa ttatagtcat aaacaattaa
     2461 caaataatgc tgaagagatt ataaaaagga ttccgacaga agctcctaaa gctaagtggg
     2521 gatctactac caatggatta actccagagc aacaaaagga gtactatctt agtaaagtag
     2581 gagaaacatt tactatgaaa gccttcatgg aggcagatga tattttgagt caagtaaatc
     2641 gaaatagtca aaaaattatt gttcatgtaa ctgatggtgt tcctacgaga tcatatgcta
     2701 ttaataattt taaactgggt gcatcatatg aaagccaatt tgaacaaatg aaaaaaaatg
     2761 gatatctaaa taaaagtaat tttctactta ctgataagcc cgaggatata aaaggaaatg
     2821 gggagagtta ctttttgttt cccttagata gttatcaaac acagataatc tctggaaact
     2881 tacaaaaact tcattattta gatttaaatc ttaattaccc taaaggtaca ttttatcgaa
     2941 atggaccagt aagagaacat ggaacaccaa ccaaacttta tataaatagt ttaaaacaga
     3001 aaaattatga catctttaat tttggtatag atatatctgg ttttagacaa gtttataatg
     3061 aggattataa gaaaaatcaa gatggtactt ttcaaaaatt gaaagaggaa gcttttgaac
     3121 tttcagatgg ggaaataaca gaactaatga agtcattctc ttctaaacct gagtattata
     3181 ccccgatagt aacttcatcc gatgcatcta acaatgaaat tttatctaaa attcagcaac
     3241 aatttgaaaa gattttaaca aaagaaaact caattgttaa tggaactata gaagatccta
     3301 tgggtgacaa aatcaattta cagcttggca acggacaaac attgcaacca agtgattata
     3361 ctttacaggg aaatgatgga agtataatga aagatagcat tgcaactggt gggcctaata
     3421 atgatggtgg aatacttaaa ggggttaaat tagaatacat caaaaataaa ctctacgtta
     3481 gaggtttgaa cttaggggag ggacaaaaag taacactcac atatgatgtg aaactagatg
     3541 acagttttat aagtaacaaa ttctatgaca ctaatggtag aacaacattg aatcctaaat
     3601 cagaggatcc taatacactt agagattttc caatccctaa aattcgtgat gtgagagaat
     3661 atcctacaat aacgattaaa aacgagaaga agttaggtga aattgaattt acaaaagttg
     3721 ataaagataa taataagttg cttctcaaag gagctacgtt tgaacttcaa gaatttaatg
     3781 aagattataa actttattta ccaataaaaa ataataattc aaaagtagtg acgggagaaa
     3841 acggcaaaat ttcttacaaa gatttgaaag atggcaaata tcagttaata gaagcagttt
     3901 cgccgaagga ttatcaaaaa attactaata aaccaatttt aacttttgaa gttgttaaag
//
LOCUS       DATRNK010000103         3900 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.104, whole genome shotgun sequence.
ACCESSION   DATRNK010000103 DATRNK010000000
VERSION     DATRNK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.104"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            55..1869
                     /gene="glmS"
                     /locus_tag="VBC52_001951"
     CDS             55..1869
                     /gene="glmS"
                     /locus_tag="VBC52_001951"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEO6883507.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVVGDNKSQL
                     VKSVGRIAELQAKVGDSVSGTTGIGHTRWATHGKPTEGNAHPHTSGSGRFVLVHNGVI
                     ENYLQIKETYLTKHNLKGETDTEIAIHLVEHFVEEDNLSVLEAFKKALHIIEGSYAFA
                     LIDSQDADTIYVAKNKSPLLIGLGNGYNMVCSDAMAMIRETSEYMEIHDKELVIVKKD
                     SVEVQDYDGNVIERGSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRKLISTYANESG
                     DMNVDSDIIKSVQEADRLYILAAGTSYHAGFAAKTMIEKLTDTPVELGVSSEWGYNMP
                     LLSKKPMFILLSQSGETADSRQVLVKANEMGIPSLTITNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQVATLAFLAKAVGEANGKAEAKDFDLVHELSIVAQSIEATLSEKD
                     VISEKVEQLLISTRNAFYIGRGNDYYVTMEAALKLKEISYIQTEGFAAGELKHGTISL
                     IEDNTPVIALISADSTIAAHTRGNIQEVVSRGANALIIVEEGLEREGDDIIVNKVHPF
                     LSAISMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
     gene            2032..2361
                     /locus_tag="VBC52_001952"
     CDS             2032..2361
                     /locus_tag="VBC52_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEO6883508.1"
                     /translation="MSLPNCPKCNSEYVYEDGILLVCPECAYEWNPEEIEEEVGLIVL
                     DSNGTRLSDGDTVTVIKDLKVKGAPKDIKQGTRVKNIRLVDGDHNIDCKIDGFGAMKL
                     KSEFVKK"
     gene            2496..3137
                     /locus_tag="VBC52_001953"
     CDS             2496..3137
                     /locus_tag="VBC52_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6883509.1"
                     /translation="MSYILEILPSLLVGAQVTLQVFFIVICLSIPLGIVVAFLMKVHF
                     KPLQWLLTTYIWIMRGTPLLLQLIFFYYFLPSVGISFDRMPAAIIAFTMNYAAYFAEI
                     FRGGIESIPKGQYEAAKVLKFSKFQTVWYIVLPQVFKIVLPSVFNETITLVKDSSLVY
                     ILGVGDLLLESKTAANRDATLAPMFIAGGIYLLLIGLLTILSKQVEKRFNYYK"
     gene            3147..3776
                     /locus_tag="VBC52_001954"
     CDS             3147..3776
                     /locus_tag="VBC52_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6883510.1"
                     /translation="MLELKNISKCYGQKEIFKDFNLTVEEGKILSLVGPSGGGKTTLL
                     RMLAGLEKIDSGTIVHDGKEVSVDHLETLNLLGFVFQDFQLFPHLTVLDNLILSPVKT
                     MGLSKELAKEKALVLLERLGLKDHALVYPFSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQEVEKLILQNRETGMTQIVVTHDLQFAESISDTILKINPK"
     gene            3790..>3900
                     /locus_tag="VBC52_001955"
     CDS             3790..>3900
                     /locus_tag="VBC52_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6883511.1"
                     /translation="MLTHKNILLTIIFGLFMIILSACGMSNKEMAGIDNWE"
BASE COUNT         1274 a          583 c          786 g         1257 t
ORIGIN      
        1 aaaagaaagt ttagatagaa agtataagag aaagctctta ttggtaataa taatatgtgt
       61 ggtatcgtag gtgtagttgg aaatacaaat gcaacggata ttttaattca aggcttagaa
      121 aagctagagt accgaggata tgactcagca ggtatttttg tagtaggtga taacaaaagt
      181 caacttgtga agtcagtcgg tcgaatcgcg gagcttcagg caaaagtagg agattctgtt
      241 agtggtacga caggtatcgg tcatacacgt tgggcaaccc atggtaaacc tactgaagga
      301 aatgcacatc ctcacacttc aggatctggt cgttttgtat tggtacacaa tggtgttatt
      361 gaaaattacc ttcaaattaa agaaacttac ttaacaaaac ataacctcaa gggtgaaact
      421 gatacagaaa tagctattca tttagttgaa cactttgttg aagaagataa cttatcagtg
      481 cttgaagcct ttaaaaaagc cttacatatt atagaaggat catacgcatt tgctcttatt
      541 gattctcaag atgctgatac gatttacgta gctaaaaata aatcacctct tctaataggt
      601 ttgggaaatg gttataatat ggtgtgttca gatgccatgg ctatgatacg tgagacaagt
      661 gaatatatgg aaatccacga taaagaactc gttattgtta aaaaagatag cgttgaagtt
      721 caagattatg atggtaatgt cattgaacgt ggtagctaca cagcagagct tgacttatca
      781 gatattggta aggggactta tccattctac atgcttaaag aaattgatga gcaaccaact
      841 gtgatgcgta agttgatttc aacttatgcg aacgaatctg gagatatgaa tgtggattct
      901 gatattataa aatcagtcca agaagcagat cgtctttaca tattggctgc aggaacgtca
      961 tatcatgctg gttttgctgc taagaccatg atcgaaaaat taactgatac acctgttgaa
     1021 cttggagttt cttcagaatg gggttacaac atgccacttt tgagtaagaa gccaatgttt
     1081 attttactta gtcaatcggg agaaacagca gatagtagac aagttttagt taaagcaaac
     1141 gaaatgggaa tccctagttt aactataaca aatgtgcctg gctcaacact ttctcgtgaa
     1201 gcaacctata cgatgctcat tcatgcagga cctgaaatcg ctgttgcttc tacaaaagca
     1261 tatactgctc aagttgcaac gttagctttc ttagcaaaag cggtaggaga agcaaatggt
     1321 aaggcagaag caaaagattt tgatttggtt catgaattgt caattgtagc acaatcaatt
     1381 gaagcgacct tatcagaaaa agatgttatc tctgagaagg tagagcagtt gttaatctcg
     1441 acacgtaatg ccttctacat tggtcgtggt aacgactatt atgttacaat ggaggctgca
     1501 ctgaagctaa aagagatttc ttatattcag acagaaggtt ttgctgcagg tgaattaaaa
     1561 catggtacaa tttcgttgat tgaagacaat actcctgtta ttgctttaat atctgctgat
     1621 tcaactattg cagcgcatac tcgtggaaat atccaagaag ttgtttctcg cggagcaaat
     1681 gctttgatta ttgtagaaga agggttggaa cgtgaaggtg atgatattat tgttaataaa
     1741 gttcacccat tcttatcagc catttcaatg gttattccga ctcaattaat tgcttactat
     1801 gcttcattac aacgtggctt agatgttgat aaaccacgta atttggcaaa agctgtaact
     1861 gttgaataga ctcgttacac taaaatacgc taatattaac tacaaaatat taaaaattgt
     1921 aaaaaagcct atttttgaag gaaaataggc tttttcctat tgtcaagaag cctataatca
     1981 gttagaatgt tacttgctga ctattttatt aattaattta aggagagaaa aatgtcatta
     2041 ccaaattgcc ctaagtgtaa ctctgaatat gtctatgaag acggtatttt actggtttgt
     2101 ccagaatgtg cttacgagtg gaatccagaa gaaatcgaag aagaagtagg tttaattgta
     2161 ttggatagca atggaacgcg cttatcagat ggagatactg tgacagttat taaggattta
     2221 aaagtaaaag gagcgcctaa ggatattaag caaggcactc gtgttaaaaa tattcgttta
     2281 gttgatggcg atcataatat cgattgtaaa attgatggtt ttggagctat gaaactaaaa
     2341 tctgagtttg taaaaaaata gctttcaggc aattggcaac ggtcaattgc tttttgaata
     2401 tgacttgatt tcatgcattt ttattaaaaa agctctatga cattggtttg aattttgata
     2461 taatgagaac atctattttt tatttagggg ggaccatgtc ttatatttta gaaatcttac
     2521 ctagcttatt agtaggtgcg caagtgacat tgcaagtttt ctttattgtt atttgtttat
     2581 caattccact tggtattgtt gtagccttcc taatgaaagt tcattttaag ccgcttcaat
     2641 ggctactgac aacttatatt tggattatgc gaggcacgcc tctgttatta cagcttatct
     2701 ttttttatta ctttttacca agtgtaggga tttcttttga tcgtatgcca gctgcgatta
     2761 ttgcttttac gatgaattat gcagcttatt ttgcagagat ttttcgagga ggtattgagt
     2821 ctattcctaa agggcaatat gaagctgcaa aggtattaaa gttttctaag tttcaaacgg
     2881 tttggtacat agtattacct caagtcttca aaatagtctt accgagtgtt ttcaatgaaa
     2941 cgattacttt agtgaaagat tcctcattgg tttacatact tggtgtgggc gacttacttc
     3001 tagaaagtaa gacagccgca aatagggatg caacacttgc accaatgttt atagcaggtg
     3061 gtatttacct attattaatt ggtcttttaa ctattttatc taaacaagtt gaaaagcgtt
     3121 ttaactacta taaataagga gggacgatgt tagaattaaa aaatatttct aaatgctatg
     3181 ggcaaaaaga aatctttaaa gatttcaatc tgactgtaga agaaggtaaa attctctctt
     3241 tagtaggacc ttctggaggt ggaaaaacaa cactattacg tatgttagct ggattggaaa
     3301 agattgattc agggactatt gttcacgatg gtaaagaagt atctgttgat catttggaaa
     3361 cactaaatct tttaggattc gtcttccaag actttcaatt attcccacat ttaacagtat
     3421 tagataattt gatcctctct cctgttaaaa cgatgggatt atcaaaagaa cttgctaaag
     3481 aaaaagcctt agtattactt gaacgcttgg gacttaaaga ccatgcttta gtctatccat
     3541 tttcattatc tggggggcaa aagcaacgtg tcgcactagc tcgtgcgatg atgattgatc
     3601 cacagattat tggttatgat gagccaacta gcgctcttga tccagagttg cgtcaagaag
     3661 tagaaaaact aattttacaa aatagagaaa caggtatgac acaaattgta gtaacacatg
     3721 atcttcaatt tgctgaaagt atatctgata cgattctcaa aattaatcct aagtagactt
     3781 gggggtagat tgttgactca caaaaatata ttattaacca ttatatttgg attatttatg
     3841 attatattat cagcatgtgg tatgtctaat aaggaaatgg ctggtattga taattgggaa
//
LOCUS       DATRNK010000104         3889 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.105, whole genome shotgun sequence.
ACCESSION   DATRNK010000104 DATRNK010000000
VERSION     DATRNK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.105"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            79..1536
                     /locus_tag="VBC52_001956"
     CDS             79..1536
                     /locus_tag="VBC52_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6883512.1"
                     /translation="MSNHSRRQQKKHSHTPLRVINLFLLVIFILLSVVSLFLMYRHHF
                     LAFRHLNVIYGVVIVLIILASLFLCIKNKARIFTTIILVLASIFVATTLYGFKSTIDL
                     TNNLNKTASYSEIEMSVVVPKDSKITNIEAVSKLAAPVKNDTSNITDLIEHIKSEEGI
                     SITPQKTDSYQDAYNRIKNGNSQAMVLNNAYVSLIELSTPDFKSQIKTIYTYKIKKKI
                     NRKNTNHKEGVFNIYISGIDTFGSISTVSRSDVNIIMTVNTNTHKVLLTTTPRDAYVK
                     IPDGGGNQYDKLTHAGLYGVETSMKTLENLYDINLDYYARINFSSFLKLIDLLGGVTV
                     YNDQAFTSKHGNFDFPVGQVTLNSEQALGFVRERYSLQGGDNDRGRNQEKVIAAIINK
                     LASSQSVTKLNSITSQLQTSVQTNMTIDNINDLINNQLSTGQRFTVESQALTGHGSTG
                     ELPSYAMPGAQLYMMSIDQSSLSNAKSKIKNTMEE"
     gene            1542..2273
                     /locus_tag="VBC52_001957"
     CDS             1542..2273
                     /locus_tag="VBC52_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase CpsB"
                     /protein_id="HEO6883513.1"
                     /translation="MIDIHSHIVFDVDDGPKTLEESLSLIEESYRQGVRIIVSTSHRR
                     KGMFETPEDIIFKNFSIVKHEAEKRFEHLQILYGGELYYTSDMLEKLKLKQIPTLNNT
                     KFALIEFSMQTSWKDIHTALSNVLMLGITPVVAHIERYNALENQKERVKEIINMGCYT
                     QINSSHILKQKLFNDKHKRFKKRARYFLEENLVHFVASDMHNLDVRPPFLAEAYKIIC
                     RDFGKERANQLFIENAQSILKNHYI"
     gene            2282..2974
                     /locus_tag="VBC52_001958"
     CDS             2282..2974
                     /locus_tag="VBC52_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEO6883514.1"
                     /translation="MNKIANTEVEINIFNLLKKLWKKKFLITFVAIAFATAGLFYSLF
                     IVTPQYTSSTRIYVINPNTPNNSITAQDLQAGSFLANDYKEIITSTDVLEKVISSEKL
                     NYPSSQLLQKITVSILKDTRVISISVEDANPKMSQKLANSVREAAVSKIKAVTQVEDI
                     TTLEKGNLPKAPSSPNIKKNVLIGFIVGAGLSTIVLVIMGILDDRVNTEEDIEKVLGL
                     TSLGIVPDLNKL"
     gene            2985..3683
                     /locus_tag="VBC52_001959"
     CDS             2985..3683
                     /locus_tag="VBC52_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEO6883515.1"
                     /translation="MTRLEIVDSKLRQAKKTEEYFNAIRTNIQFSGKENKILAITSFR
                     EGEGKSTTSTSLALSLAQAGFKTLLIDADTRNSVMSGTFKATGTIKGLTNYLSGNADL
                     GDIICETNVPRLMVVPSGKVPPNPTALLQNAYFNKMIEAIKNIFDYIIIDTPPIGLVV
                     DAAIIANACDGFILVTQAGRIKRNYVEKAKEQMEQSGSKFLGIILNKVNESVATYGDY
                     GDYGNYGKRDRKRK"
     gene            3696..>3889
                     /locus_tag="VBC52_001960"
     CDS             3696..>3889
                     /locus_tag="VBC52_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883516.1"
                     /translation="MKEKENIQKIIIAMIQTVVVYFSASLTLTLITPNFKSNKDLLFV
                     LLIHYIVFYLSDFYRDFWSRG"
BASE COUNT         1397 a          566 c          656 g         1270 t
ORIGIN      
        1 ctttcgttga atattgtatt gaaaaatgat ataatatcca agtaaaatta aggttttttt
       61 aagtattgga gaattacaat gtctaatcat tcgcgccgtc aacaaaagaa acactcacat
      121 acacctctac gggtgattaa tttatttctt ttggtgattt ttattttgtt aagtgtagtc
      181 tcattatttc ttatgtatcg tcaccatttt ttggcattta gacacctgaa cgtcatttat
      241 ggagttgtaa ttgttttaat cattttagca agtttatttc tttgtattaa gaataaagct
      301 agaattttta caactataat tttagtatta gcttctattt tcgttgctac tactttatat
      361 ggatttaagt caaccattga tttgacaaat aatctaaata aaactgcttc atactctgaa
      421 attgagatga gtgtagttgt accaaaagat tctaaaataa ccaatataga agctgtcagc
      481 aaattagccg caccagttaa aaacgatact tcaaatatta ctgatttgat agaacatata
      541 aaatcagaag aaggaatctc tattacacca caaaaaacag attcttacca ggatgcatac
      601 aatagaatta aaaatggtaa tagtcaggct atggttttaa ataatgctta tgttagctta
      661 attgaactta gcacccctga ttttaaatcg cagataaaaa cgatttatac ttacaaaatt
      721 aagaaaaaaa ttaatcgtaa aaatactaat cataaagaag gggtatttaa tatctatatt
      781 agcggtattg atacttttgg ctctatatca acagtatcaa gatctgatgt aaatattatt
      841 atgacggtta ataccaatac ccacaaagta ttgttaacga caacaccacg agatgcctat
      901 gtaaaaattc cagatggtgg gggcaatcaa tatgataaat taacccatgc aggtttgtat
      961 ggcgttgaga catcaatgaa aacacttgaa aacctttacg acatcaacct tgattattat
     1021 gctagaatta atttttcatc atttttaaaa ttaatagacc tcttgggagg agtgacagtt
     1081 tataacgatc aagcttttac aagtaaacat ggtaattttg acttccctgt tggtcaagta
     1141 acattgaatt ctgagcaggc tttgggcttt gttagagaac gttattctct acaaggaggc
     1201 gataacgata gaggtagaaa tcaagaaaaa gtgattgcag ctattataaa taagttagct
     1261 tctagtcagt cagtaacaaa attaaatagc attacctcac agctccaaac gtccgttcaa
     1321 actaatatga ctattgataa tattaatgat ttgattaaca atcaattgtc aactggacaa
     1381 cgcttcactg tcgagtcaca agcattaact ggtcatggtt caacgggtga actcccttca
     1441 tatgcaatgc caggagctca actttatatg atgtcaattg atcaatctag cttatctaat
     1501 gcaaaatcaa aaattaagaa cacaatggag gaataacaag aatgattgat attcattctc
     1561 atatcgtttt tgatgtagat gatgggccta aaacgcttga agagagttta tctttgatag
     1621 aagaaagtta tcgacaaggt gttagaataa ttgtttcgac atcacataga agaaaaggta
     1681 tgtttgaaac acctgaagat attatcttca aaaacttttc tatagttaaa cacgaagcgg
     1741 aaaaaagatt tgaacatctt caaatcttat atggtggaga attgtactat acaagtgata
     1801 tgttagaaaa actgaagtta aagcaaattc caactttaaa caatacgaaa tttgctttaa
     1861 ttgaattttc tatgcaaact tcttggaaag atattcatac agctttgtca aatgttttaa
     1921 tgcttggtat tacaccagtc gttgcgcata tagagaggta taacgcttta gagaatcaaa
     1981 aagaacgggt gaaggaaatt attaatatgg ggtgttacac acaaataaat agttctcata
     2041 ttttgaaaca aaaacttttt aatgataagc ataaacgctt taagaaaaga gcccgttatt
     2101 ttttagagga aaatttagtg cattttgtag cgagtgatat gcataacctt gatgttagac
     2161 cgccattttt agcagaagct tataagatta tctgtagaga tttcggtaaa gaacgtgcta
     2221 accaactttt tattgaaaat gctcaatcta tattaaaaaa ccattacatt taggagattt
     2281 catgaataaa atagctaata cagaagttga aataaatata ttcaaccttt taaaaaaatt
     2341 gtggaaaaag aaatttttaa tcacatttgt tgccattgcg tttgcaacag ctggactttt
     2401 ctatagtctt tttattgtta cgccacaata tacttcttca acaaggatat atgtcattaa
     2461 ccctaatacc cctaataata gtattactgc acaagatcta caagcgggga gttttcttgc
     2521 aaatgactat aaggagatta ttacgtctac tgacgttcta gaaaaagtta tttcttctga
     2581 aaaattgaat tatccttcgt ctcagttgct gcaaaaaata acagtttcta ttttaaaaga
     2641 tacacgtgtt atttcaatat cggtcgaaga tgctaatcca aaaatgtctc aaaaattagc
     2701 aaattcagtt agagaagcag cagtttcaaa aatcaaggca gttactcaag tagaggatat
     2761 cactactctt gagaagggaa atttacctaa agcaccatct tctcctaata ttaaaaagaa
     2821 tgtactaatc gggtttattg ttggtgcagg attatcaact attgttttag ttattatggg
     2881 tattttggat gatcgtgtta atactgaaga agatattgaa aaggtcctag gacttacttc
     2941 tttaggtata gtaccagatt tgaataaact ttaaggagaa tataatgact cgtttagaaa
     3001 tagttgatag caagttaaga caagcaaaaa aaacagaaga atacttcaat gcgatccgta
     3061 caaacataca gtttagtgga aaggaaaata aaattcttgc aattacctct tttagggaag
     3121 gggaaggaaa atccactact tcaacaagtt tagctttatc tttagctcaa gcaggattta
     3181 aaacattatt aattgatgcg gatactagga actctgttat gtctggaacc tttaaagcaa
     3241 ctggaactat taaaggcttg acgaattatt tatcaggtaa tgcagatctt ggagatatta
     3301 tctgtgaaac caatgttcct agactgatgg tcgttccttc agggaaagta ccaccaaatc
     3361 caacagcatt acttcagaac gcttatttta ataagatgat tgaagctatt aaaaatatat
     3421 ttgattatat tatcatcgat actccaccta ttggtttagt tgttgatgcc gcaataatcg
     3481 ctaatgcttg cgatggtttt attttagtaa cccaagcagg tagaataaaa cgtaattatg
     3541 ttgaaaaagc aaaagaacag atggaacaaa gtggttcaaa gttcttaggt attattctta
     3601 ataaagttaa tgaatctgtt gctacttacg gcgattacgg cgattatgga aattacggaa
     3661 aaagggatag aaaaaggaag taaggagctc ttgtattgaa agaaaaagaa aatatacaaa
     3721 agattattat agcgatgatt caaaccgttg tggtttattt ttctgcaagt ttgacattaa
     3781 cattaattac tcccaacttt aaaagcaata aagatttatt gtttgttcta ttgatacatt
     3841 atattgtctt ttatctttct gatttttaca gagacttttg gagtcgtgg
//
LOCUS       DATRNK010000105         3889 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.106, whole genome shotgun sequence.
ACCESSION   DATRNK010000105 DATRNK010000000
VERSION     DATRNK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.106"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(66..1493)
                     /locus_tag="VBC52_001961"
     CDS             complement(66..1493)
                     /locus_tag="VBC52_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO6883517.1"
                     /translation="MTKEYQNYVNGEWKSSVNQIEILSPIDDSSLGFVPAMTREEVDH
                     AMKAGREALPAWAALTVYERAQYLHKAADIIERDKEEIATVLAKEISKAYNASVTEVV
                     RTADLIRYAAEEGIRLSTSADEGGKMDASTGHKLAVIRRQPVGIVLAIAPYNYPVNLS
                     GSKIAPALIGGNVVMFKPPTQGSVSGLVLAKAFAEAGLPAGVFNTITGRGSEIGDYIV
                     EHEEVNFINFTGSTPVGKRIGKLAGMRPIMLELGGKDAGVVLADADLDNAAKQIVAGA
                     YDYSGQRCTAIKRVLVVEEVADELAEKISENVAKLSVGDPFDNATVTPVIDDNSADFI
                     ESLVVDARQKGAKELNEFKRDGRLLTPGLFDHVTLDMKLAWEEPFGPILPIIRVKDAE
                     EAVAIANKSDFGLQSSVFTRDFQKAFDIANKLEVGTVHINNKTGRGPDNFPFLGLKGS
                     GAGVQGIRYSIEAMTNVKSIVFDMK"
     gene            complement(1643..3376)
                     /gene="ptsP"
                     /locus_tag="VBC52_001962"
     CDS             complement(1643..3376)
                     /gene="ptsP"
                     /locus_tag="VBC52_001962"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEO6883518.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVEDTNAEEARL
                     DVALQASQDELSVIREKAVESLGEEAAAVFDAHLMVLSDPEMINQIKETIRAKQVNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITERVQDGQ
                     LIAVNGITGEVIIEPTEAQISAFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNENGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAIFEEEKANLLADGVAVAEGIEVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALSECSTMEEV
                     IELQKEYVDFD"
     gene            complement(3381..3644)
                     /locus_tag="VBC52_001963"
     CDS             complement(3381..3644)
                     /locus_tag="VBC52_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEO6883519.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAIEETMTKEGLA"
BASE COUNT         1150 a          813 c          661 g         1265 t
ORIGIN      
        1 tttattttta atgtaaaaga ggtttataaa acgactcaac tgacaatagt tgagtcgttt
       61 tacgtctatt tcatatcaaa aactatcgat tttacatttg tcattgcttc aattgaatat
      121 ctgatacctt gaacacctgc accagatcct ttgagtccta agaatgggaa attatcagga
      181 ccacgtccag tcttattgtt aatgtgaact gtaccaactt caagtttatt tgctatatca
      241 aatgcttttt ggaaatcacg tgtaaagact gatgattgta atccaaaatc agatttgttg
      301 gcaatagcaa cagcttcttc tgcatccttg acacgaataa ttgggagaat tggtccaaaa
      361 ggctcttccc aagctagttt catatctaaa gtaacatgat caaacaatcc tggagttaat
      421 agacgaccat cacgtttaaa ttcattcaat tctttcgcac ctttttgacg tgcatctact
      481 actaagcttt caataaagtc ggctgaatta tcatcaataa ccggtgtcac cgttgcatta
      541 tcaaatggat cacctactga taattttgct acattttcag atattttttc tgccaattca
      601 tctgcaactt cttcaacgac aagtacacgc ttaattgccg tacagcgttg tccggagtaa
      661 tcataagcac ctgcaacgat ttgtttagca gcgttgtcaa ggtcagcatc agctaagacg
      721 acacctgcat ccttaccacc caactcaagc ataattggac gcattcctgc taacttacca
      781 atacgttttc caactggcgt tgatcctgta aagttaataa aattaacttc ttcatgctca
      841 acaatgtaat ctccaatctc agaaccgcgt cctgtaatag tattaaagac acctgctgga
      901 agacctgctt ctgcaaaagc ttttgctaaa acaagtcctg agactgaacc ttgtgttggt
      961 ggtttaaaca tcacaacgtt tccaccaatt agagctggcg caatttttga tcctgagagg
     1021 ttaacagggt aattataagg tgcgattgct aaaacgatac ctactggttg acgacgaata
     1081 acagccaact tatgacctgt tgaagcatcc atttttccac cttcgtcagc tgaagttgat
     1141 aaacgaattc cttcttctgc tgcataacga ataagatcag ctgtccttac aacctcagtt
     1201 actgaagcat tgtaagcttt agaaatttct tttgctaaaa cagtagcaat ttcttcttta
     1261 tcacgttcaa taatgtctgc ggctttatga aggtattgtg cacgttcata tactgtcaaa
     1321 gcagcccagg ctggtaaagc ctcacgaccc gctttcatag catgatcaac ttcttctcga
     1381 gtcatcgctg gcacgaatcc caatgaagaa tcatcaattg gtgacaaaat ctcaatctga
     1441 ttaacagatg atttccattc gccattgaca taattttgat attcttttgt caaagtaaat
     1501 ctccttttac attgtaatag gtttattcta tcactttatc tatttatttc aattatctga
     1561 catatttttc ttagaaactt aacaatatcc aaactttcaa taaaaaagta gagatttttt
     1621 aatccctact tttttattat tctcagtcaa agtcaacgta ttctttttga agttcgataa
     1681 cttcttccat agttgaacac tctgaaagag cacgattagc gtattcttcc attttagctg
     1741 tgtccaattt tttcattaag ctacgtgtac gaagtactga ggttgctgac attgagaatt
     1801 catccaatcc cataccaaca agaagtggaa ccgcagtttg atcaccggcc atctcaccac
     1861 acatacctgc ccatttacct tcagcatgag cagctttgat aacgttgtta atcaaacgaa
     1921 ggattgatgg attgtatggt tgatagaggt atgaaacttg ctcattcata cggtccgcag
     1981 ccattgtgta ttggataaga tcattagtac caattgagaa gaaatcaact tcttttgcaa
     2041 attggtctgc aagcatagct gccgctggga tttcaatcat aataccaact tcaatgcctt
     2101 cagctactgc tacaccgtca gctaacaagt tcgctttctc ttcttcaaat atagctttag
     2161 cagcacggaa ttctttaagc aaagcaacca ttgggaacat gatgcgtagc tgtccatgaa
     2221 ctgaagcacg aagaagtgct cggatttgtg tgcggaacat tgcatcacca gtttcagaaa
     2281 ttgaaatacg caatgcacgg aatcctaaga atgggttcat ctcttttgga agatcaaagt
     2341 aaggaagctc tttatctcca ccgatatcca ttgtacgaac aacaactggt ttaccattca
     2401 taccctcaag aactgcttta tatgcttcat attgctcatc ttctgttggg aaatcttgtg
     2461 aatccatata taagaactct gtacggtaaa gaccaactgc ttctgctcca ttttcgttaa
     2521 caccctcaac atctttaggt gtaccgatat tagcagctaa ttcaaaatgt ttaccatcag
     2581 cagtaactgt ttgtgcatcc ttaagaagtg cccattcagc tttttgtttg gcataagctt
     2641 cacctgctgc cttgaaggct gaaatttgag cttcagttgg ttcaatgata acttcaccag
     2701 taataccatt aacagcaatc aactgaccat cttgaacacg ttcagtaata tcatttgtac
     2761 caagaacagc tgcgatttcg agtgtacgtg ccataatagc tgagtgactt gtacgccctc
     2821 caatatttgt tacaaaagct ttaacaaatt gtttatttaa ttgagctgta tcagaaggtg
     2881 tcaaatcgtg tgcaataacg attgattctt cattgatagt tgctgggttt ggtaatttaa
     2941 cacccaaaag atgtgctaag acacgtttgg caacatcacg gatatctgct gcacgttctt
     3001 gcatgtatgg gttatcttcc ataccttcaa agatagtgat gaacatatca gtcacttctt
     3061 taagacctgt ttctgcattt acttgttttg cacgaatagt ctctttaatt tgattaatca
     3121 tttctgggtc agaaagaacc attaaatgag catcaaatac agctgcggct tcttcgccta
     3181 agctttctac tgccttctca cggataacag aaagctcgtc ttgtgaagct tggagtgcaa
     3241 catcaaggcg agcttcttct gcgtttgtat cttcgactgt tacagtctca aatgacaaat
     3301 ccggttggac aagtagatat gctttagcaa ctgcaacacc atcagaggct gcgattcctt
     3361 taagcatttc tgtcattttc ttatgccaat ccttcttttg tcattgtttc ttcgatagct
     3421 gcaattgcat catctgcatc tgcaccttct gctgaaatag taacatcagc accttgacca
     3481 acaccaagac tcataacacc catgattgat ttaaggttta ctgctttacc tttgtaatca
     3541 agcgtgatgt ctgaagcaaa tttgctagct gtttggacaa gcaaagtagc tggacgtgcg
     3601 tgaatacctg tttctgcaac aatgtggaaa tcttttgaag ccatattatg gttctccttt
     3661 tgtcatttgt aaatatttga gtcacctatt ggtaaccctt acaagttaaa agtataacat
     3721 attgcttttt atttttcaag attaaatgtc tcaaacagaa aaaaacttga atgttttcca
     3781 cagcttttca tattaaaaat gagagtctta gaatttaaaa atagaattct aaaaatgatt
     3841 tttttatgtt cttctgaaag gaattggaaa acaggctgaa agcttttaa
//
LOCUS       DATRNK010000106         3843 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.107, whole genome shotgun sequence.
ACCESSION   DATRNK010000106 DATRNK010000000
VERSION     DATRNK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.107"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            167..>3843
                     /locus_tag="VBC52_001964"
     CDS             167..>3843
                     /locus_tag="VBC52_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YPDG domain-containing protein"
                     /protein_id="HEO6883520.1"
                     /translation="MLRKYKQRNYDVAHTKQRFSIKKFKFGAASVLVGLTFLGMAGTT
                     VLADETTSTGIELKQSASAGIVDEVKPAKSELATDAIASVDKTTESEVEALADYTENV
                     NTDVGGTVPESSANIDPNSSSKDTDKVAVLEETASEKNVIVSPAITTRSVDSAVEKAN
                     VDKNSVSEVSEELQKRVQKDAIYSPGDSSAKQTYSGKAWIDNGGSIDGQASDDIPLAG
                     VKVYLQWVNGKGYVSKVYYTTTLADGTFAFDLSKSEVDPNGEEHHFQLAGDANFAVRT
                     WIENPDSSKYDIIQAGDKIYGFHTRTNRKNESWDFTAGVNRIVNSQVILQEKKLTKDW
                     LKKPEAESQIPSTADGTWHDRGIYGRVNGLAWFDNGDSAGTLANQWIKDGNDVPANGV
                     KVAASYLNDEVTRLLDQWKKDHPGYTIEDFKAEQERVFTEYQAQHGVGSHIAETVITT
                     VDKNGNYSIPFKGLYGVSAYKANSGLKVSHTITDEEFGKVVRDEDVDNSSLLKWNGTI
                     GQKHRHINENYLYVSPILSNYNIWSPAFPKAMFGNPNNLFTNVVAGSGTNITSLNFAI
                     LAPQPMLDIVDYDTTNKVAVSGNVTNSTVGGLLPTREYQVQWFRDGVAIGEPQTIQSS
                     VEGTATPEKFTVPNDITKTTNFTLGLFEQGQNTKSLGNALALDSFIAVFIEYKPVDGV
                     VAKDSEPAKPIFKDKDGNDTTPPEGTKFTTVAGQVPDSVKTSYPEGAMSVDPNMVTID
                     PTTGVVVVKGEALTKKGTYVVPVEVTYPGGSKAYTFATVNVKDDATTTYTATGGKLEK
                     PFGQAPSTDEITSKVTTDYPKEVKDQPTMKVKEGAVIPDGMTKGEFKVPVVVTYPDGT
                     TDEVDVTVTIKESEAEKNAPSYKDTMVVPGKPAESTPSYGEGKTAPNGTEYKIDENFK
                     VPDGYKVDIDKATGTVTVTAPEKSDGTTAEEIEVPVIVTYPDKSEDKVIAKFQLDTDE
                     NGTPDVTDPDDDGDGIPDVDDKNPKTATKTAVAVDNVIVTEGQPIAPIPVTVTSDDRN
                     AKVEVSGLPDGLTYDSATCQITGTPDKLADWKDTEETRDFPVTVKVTDGTGTVEKTFT
                     ITVQRDTDGDGMPDVTDTDDDGDGIPDDTEKIDGTNSKDPNSAASVITPLDDQTGTVG
                     EAITPVTVKVDKVPTDGSVTVTGLPDGVTYDPATGQITGIPTKEGESTVTVAVLGKDG
                     KPVMGVDGKPVTEEFKFTVKK"
BASE COUNT         1349 a          645 c          826 g         1023 t
ORIGIN      
        1 attatcacct ttataaatct tatcgaatgt gattatacaa tacaaattca aataatagtt
       61 cacatttatc aagataatgt aaactactaa aacctttaaa aaagtaaaat gtgaaagata
      121 acttctacta gttaatctaa ttttactttt gaaaaggaga gaaattatgc ttaggaagta
      181 taagcaaaga aattatgatg tcgcacatac gaaacagcgc ttttcgatta agaaatttaa
      241 atttggtgca gcatcggtac tggttggatt gacatttcta ggaatggctg ggacaactgt
      301 tctagctgat gagacaacat ctacaggtat tgagcttaaa caatctgcat cagcagggat
      361 agtagatgaa gtaaaaccag caaaatcaga attagcgaca gatgcaatag cgtcagtaga
      421 caaaacaacc gaatcagaag tcgaagcatt ggcagattac acagaaaatg tcaatactga
      481 tgttggaggt acggttcctg agtctagtgc caacatagat cctaatagtt catcaaaaga
      541 tacagataaa gttgctgttt tagaagagac agccagtgaa aaaaatgtaa ttgtatctcc
      601 tgcaattaca acacgttctg tagatagtgc agttgaaaaa gctaatgttg acaaaaatag
      661 tgtcagcgaa gtgtctgaag agcttcagaa gagagtacaa aaagatgcga tatatagccc
      721 cggagattcc tcagctaaac aaacttattc aggtaaagct tggatagata atggtggtag
      781 cattgatggg caagcttcag atgatattcc actagctggt gttaaagttt acttgcagtg
      841 ggtaaacgga aaaggctatg tttcaaaagt ttactatact actacattgg cggatggaac
      901 ctttgcgttt gacttatcta aatcggaagt tgatcctaac ggtgaagaac atcattttca
      961 attagcagga gatgctaatt tcgcagtaag aacctggatt gaaaatcctg attctagtaa
     1021 gtatgatatt attcaagcag gggataagat ttatggtttc catactcgta ctaaccgtaa
     1081 aaatgaatct tgggatttta ctgcgggagt caataggatt gtcaattctc aagttatttt
     1141 acaagaaaag aaattaacta aagactggtt gaagaaacca gaagctgagt cgcaaattcc
     1201 tagtaccgca gatggtacat ggcatgacag gggaatctat ggtagagtaa atggtttagc
     1261 ttggtttgat aatggagatt ctgctgggac gcttgctaat caatggataa aagatggtaa
     1321 tgatgttcca gcaaatggtg tgaaagtagc tgcttcttat ttaaatgatg aagtaactcg
     1381 cttacttgat cagtggaaaa aagaccatcc aggatatact attgaagact ttaaggctga
     1441 acaagaaaga gtctttacag aataccaggc ccaacatggg gttggttctc atattgcgga
     1501 gacagtcata acgacagttg ataaaaatgg aaattactct attcctttta agggattgta
     1561 tggtgttagt gcttataaag caaacagtgg tttaaaagtt tctcatacaa ttacagacga
     1621 agaattcggc aaggttgtta gagatgaaga tgtagataat agtagtcttc ttaagtggaa
     1681 tggtaccatt ggtcaaaaac atagacacat taatgagaac tacctatatg tatctcctat
     1741 tttatctaat tataatattt ggagtccggc ttttcctaaa gctatgtttg gtaatccgaa
     1801 taatctattt acaaatgtag ttgcggggtc tggaactaat attaccagct tgaactttgc
     1861 tattttagca cctcaaccaa tgcttgatat tgttgattat gatactacga ataaggtagc
     1921 agtctctggt aatgttacca attcaacagt aggcggattg ttgccaacac gtgaatacca
     1981 agtgcaatgg ttcagagatg gtgttgcaat aggtgaacct caaactattc agtcaagcgt
     2041 tgaaggaaca gcgacacctg agaagtttac agtaccaaat gatatcacta agacaacaaa
     2101 ctttacatta ggtctatttg aacaaggtca aaatacaaag agcttaggta acgctttagc
     2161 attagattcc tttattgctg tatttatcga gtataaacca gtagatggtg ttgtagctaa
     2221 ggattctgag ccagctaaac caatctttaa agacaaagat ggcaatgata caacgccacc
     2281 ggaaggaaca aaattcacaa ctgttgcagg tcaagttcca gattcggtta aaacctctta
     2341 tcctgaggga gcaatgtctg ttgacccaaa tatggtaacg attgacccta caactggagt
     2401 agttgttgta aaaggggaag ctcttactaa gaaaggcaca tatgtagtac cagtagaagt
     2461 aacttatccg ggtggttcca aagcttatac ttttgctact gtaaacgtta aagatgatgc
     2521 aacaacaaca tacacagcga caggtggaaa attagaaaaa ccatttggtc aagcaccatc
     2581 aactgatgaa atcacatcaa aagtaacaac tgattatcct aaagaagtta aagaccaacc
     2641 tacaatgaag gttaaagagg gtgcagtgat tccggatggt atgaccaaag gggaatttaa
     2701 agtaccggtt gttgtcactt atccagatgg aacaacagac gaagttgatg ttacagtaac
     2761 catcaaagaa tctgaagcgg aaaagaatgc cccttcatac aaagatacaa tggtcgttcc
     2821 agggaaacca gcagaatcaa ctccaagcta cggcgaaggt aaaacagcac caaatggtac
     2881 agagtataag attgatgaaa actttaaagt accagacgga tataaagttg acattgataa
     2941 agctactgga acagtgactg taacagctcc agaaaaatca gacggtacaa cagcagaaga
     3001 aattgaggta ccagtaattg tcacttatcc agataaatca gaagataaag taatagctaa
     3061 attccaattg gatactgatg aaaatggtac accagacgtt actgatccag atgatgatgg
     3121 cgatggtatt ccagatgtgg atgataaaaa ccctaaaaca gccactaaga cagccgtagc
     3181 ggttgacaat gtaattgtta cagaaggtca accaatcgct ccgattcctg ttacggtaac
     3241 aagcgatgat aggaatgcta aggttgaagt ttctggtctt ccagacggtt taacttatga
     3301 ctcagcaaca tgtcaaatca ctggtacacc agataagtta gcagactgga aagatactga
     3361 agaaacacgt gacttcccag taactgttaa agtgactgat ggaacaggta cggttgaaaa
     3421 aacatttaca atcactgttc agcgtgatac ggatggtgat ggtatgccag atgtgacaga
     3481 tacggacgat gatggcgacg gtattccaga cgatactgaa aagattgacg gaacaaactc
     3541 taaagatcca aattcagcag cttctgttat cacaccactt gatgatcaaa caggtactgt
     3601 cggagaagcc attacaccag ttactgtgaa agttgacaaa gtgccaacag atggttcagt
     3661 tactgttact ggtcttccag atggcgttac ttacgatcca gcaacaggcc aaatcactgg
     3721 gataccaaca aaagaaggtg aatcaacggt tactgtggct gttcttggta aagatggtaa
     3781 accagttatg ggtgttgacg gcaaaccagt tacagaagag ttcaagttta cagttaagaa
     3841 att
//
LOCUS       DATRNK010000107         3647 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.108, whole genome shotgun sequence.
ACCESSION   DATRNK010000107 DATRNK010000000
VERSION     DATRNK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.108"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(187..767)
                     /locus_tag="VBC52_001965"
                     /pseudo
     CDS             complement(187..767)
                     /locus_tag="VBC52_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865706.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(803..1069)
                     /locus_tag="VBC52_001966"
     CDS             complement(803..1069)
                     /locus_tag="VBC52_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001881173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEO6883521.1"
                     /translation="MAYFRKRDNGWEYRISYKHIIGNILLCNITSTKYQQVINHYSQT
                     HVQDTVKRFNIHVKACVSMAVHEGYIEKNFCLFTKVKAKNKGRK"
     gene            1069..1473
                     /locus_tag="VBC52_001967"
     CDS             1069..1473
                     /locus_tag="VBC52_001967"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO6883522.1"
                     /translation="MIYSTLAKEQGVQGYLDGKGSLRDICKWYDISSRSVLQKWIKRY
                     TSGEDLKATSRGYSRMKQGRQATFEERVEIVNYTIAHGKDYQAAIEKFGVSYQQIYSW
                     VRKLEKNGSQGLVDRRVKGLESRPDLTEIEQL"
     gene            1635..1835
                     /locus_tag="VBC52_001968"
     CDS             1635..1835
                     /locus_tag="VBC52_001968"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6883523.1"
                     /translation="MPIQALCQLLKGSRSGYYKWLNRQKTDFETKNTKLMAKIKELHR
                     LYNGILGYRRMTTFINRQLGTT"
     gene            1860..2117
                     /locus_tag="VBC52_001969"
     CDS             1860..2117
                     /locus_tag="VBC52_001969"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6883524.1"
                     /translation="MNILGISSVIRRVSHACTKAGDRFYEENILNREFTATAHNQKWC
                     TDVTYLQYGLGAKAYLSAIKDLYNGSIIAYEISHNNEIHLL"
     gene            2243..2452
                     /locus_tag="VBC52_001970"
     CDS             2243..2452
                     /locus_tag="VBC52_001970"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6883525.1"
                     /translation="MSRIGKCIDNAPTESFFGFFKTESYHLKKYNSYDELVNDVARYI
                     EFYNTQRYQSKLNNLTPLEFRNQVA"
     gene            complement(2625..2786)
                     /locus_tag="VBC52_001971"
     CDS             complement(2625..2786)
                     /locus_tag="VBC52_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HEO6883526.1"
                     /translation="MGGIGAHKFYAGKIGQVFLYIIFRITFIPGIIDLIEALMALGKQ
                     TDEYGNIIV"
BASE COUNT         1215 a          569 c          672 g         1191 t
ORIGIN      
        1 gattatgtgt tgccgcacga ggaagtgaga gatgatgaag ttgtgacagt tgagagtgta
       61 gaagaggtgt tgagggaatt gaagtgaaag ggtgcaaaaa aggggcaaaa tagattaaaa
      121 atgaaactac attgaacttt ttactattat aaacaaccat taaatcaacg cttggactat
      181 tatgaactat tgtgtacaat agtagtagat tccggcaggg gacatattca ttgcatgtaa
      241 atagcggttt agagctattt tgccccaaat tcgccccaaa tttctctgat taagtttatc
      301 gttcctatct ttttgttctt gtaattgatg tgcgtaaact tctaaagtga tatttaaatt
      361 ctcgtgatct aaaacttgag agatggaaat tagatagctt gcaaatgtat gcctgagaga
      421 gtgcactcgt acctcgcgac cagttatttt tcggatagtt ttattgactg cattatttga
      481 aagtttgtcg aataatctgt cgtttttatt ttttgtaaat tcatgcaaaa aaaataatgt
      541 atcattgtca attggtatat ttctgatact acttttgttt tttgttggca ggtatctttg
      601 gttgaaatga taatcccaag ttttattaat tgataaatat ttgttagtgt aatcaatatc
      661 attaactgtt aaacctaaac attcagcgaa gcgcatgcca gttttagcga tgaggtataa
      721 cgctgcatac gattgatgtt gtgattttct ttacaaattt ttatcaagcg taagtattca
      781 ttggtttcaa gaaattttat ctctatttac gccccttatt ttttgcttta accttagtga
      841 ataaacaaaa atttttttct atatatccct cgtgaacagc catggatacg caggctttta
      901 catgtatgtt aaaacgcttt actgtatctt gcacatgcgt ttgactataa tgatttatga
      961 cttgttgata tttagtggaa gtaatattgc aaagtaatat atttcctatt atatgtttat
     1021 acgatattcg atattcccac ccgttgtcgc gtttacggaa atacgccatt gatatactcc
     1081 acattagcta aagaacaggg tgttcaaggc taccttgatg gaaaaggctc tcttagagat
     1141 atttgtaaat ggtatgatat ctcaagtcgc tccgttctcc aaaagtggat aaaacggtat
     1201 actagtggtg aagacttgaa agccactagt agaggatata gccgtatgaa acaaggaagg
     1261 caagccacat ttgaagaacg tgtagagatt gttaactaca ccattgccca tgggaaagac
     1321 tatcaagcag ctattgagaa gtttggtgtt tcctaccaac aaatttattc ttgggtgcgt
     1381 aagcttgaga agaatggctc acaaggtttg gttgatagac gtgtgaaagg gttggagagt
     1441 aggcctgatt taaccgagat tgagcaactt taactcaaga ttaaacaatt ggaggaacgt
     1501 aatcgtctct tagaaatcga ggttagttta ctaaaaaagt tagaagacat caaacgagga
     1561 aacagacggt aagactaggt aagcatttag cggagttcca agtaatcaag aattattacg
     1621 atgaggaatc taatgtgcct attcaggcct tatgccaact cttgaagggg tctcgttcag
     1681 gctattacaa gtggctcaat cgtcaaaaaa cagattttga gacaaaaaat acaaagctaa
     1741 tggctaaaat caaggaactt catagactct acaatggtat cttaggttat cgccgtatga
     1801 caacatttat taatcgtcaa cttgggacaa cttaaaacaa gaaacggatt cgttgattga
     1861 tgaacattct ggggattagt tcagtcattc gtcgtgttag ccatgcttgt acaaaagctg
     1921 gtgacagatt ttacgaagaa aatattctta atcgtgaatt tacagccaca gctcataacc
     1981 agaaatggtg cacagatgtc acctatcttc aatacggtct gggagctaaa gcttatctca
     2041 gtgcgattaa agacctgtat aacggttcta ttatcgctta tgagattagt cacaacaatg
     2101 aaatccactt gttatgaaga ccattaaaaa ggggctagag ctcaatccag gagccacacc
     2161 tatcatccat agcgattgag gtagtcaata tacttccaaa gaataccgtt atatcataca
     2221 acaagctggt ctgaccttat ccatgtcccg gattggcaaa tgtattgata atgcaccaac
     2281 tgaaagtttc tttgggtttt tcaagactga gtcttaccac cttaagaaat acaactctta
     2341 tgatgagttg gtcaatgatg tggcacgtta tatcgaattc tacaacacac aacgttatca
     2401 atcaaaatta aacaacctga ctcctctaga attcaggaat caggttgcat aacttatctt
     2461 ttattatttg actgtctact tgacagggag ccgttcagat tgcttaacct ttctaaattt
     2521 gctaaaatag ctacaagaaa acgagccatt taatgcttat ttcttatact gtcttgcctc
     2581 acgctctcct cgaccaaaaa ttgagcgtga ggctttttgt ttcattaaac gatgatattt
     2641 ccatattcat cagtttgttt tccgagagcc atcaaagctt cgataaggtc gataattcca
     2701 ggaataaagg taattctaaa aataatatat aaaaaaacct ggcctatttt tcctgcgtaa
     2761 aatttatgcg ctccaatgcc gcccaaaaga acgttaataa aacataaact actatgttag
     2821 cataagactt tatttttaca actgaatttc atataaatgg attagagtaa gggataaaag
     2881 aaattagcat agctcttttg aaaataaaaa aattaatata atatggaaaa aattttattt
     2941 cataaacgtt tcataaaagg tatgtaatct agtatttagg caacactatt ttgtcactgg
     3001 tgtctagtaa cttatagatt gataatttta ctagtaaacg taattcttcg ctttaagagt
     3061 taaatgtcta tttattgtaa gctaaattgg gaggtgaact tatgtaaaat tagataggta
     3121 ctgtcaagta cgggatgatt attgaaacag ccagtatgca tcataaaatc tgtattgctt
     3181 aataactatt tccttaacca gacatcagtt cattgtttat catcgctacc ctaagtctag
     3241 ttttttcaat agagcattag gtagtttttg ataataaaac tatataaaca tgagaattag
     3301 atttcgtatc gcattcttca taatgagtta tttgagattt tcctttgaat aaatagatac
     3361 gaaattcagt aacttcatat ataaacggtt ctatcattga gatagtttgt caaatgaaga
     3421 aattttaatg gaaatagttt taaaaacatt agttgtaggc gatgtaaaaa tattaatcca
     3481 gtggatgcaa tagttgcgga gtaaaaatag agaggagtaa ttaggaagtg ataaaaaatg
     3541 ctatagcata tattaccaga aaaaaaaata gaacacttat tatatttgct attttaacaa
     3601 ttgttctttc ttgcttgtat tcatgtttaa caataatgaa atcaagt
//
LOCUS       DATRNK010000108         3610 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.109, whole genome shotgun sequence.
ACCESSION   DATRNK010000108 DATRNK010000000
VERSION     DATRNK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.109"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            176..3424
                     /locus_tag="VBC52_001972"
     CDS             176..3424
                     /locus_tag="VBC52_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HEO6883527.1"
                     /translation="MSKKTFGKQLTVVGSKSRVKMHKSGKNWVRTIMSHFNLFKAIKG
                     RATVEADVCVQNIEKEDRLSSGNLTYLKGLLAAGALLGGASLTSRVYADETPVVQEQS
                     SSVPTLAEQTEVTVKTTAVQNYQDGTVSKNIIDSNSVSMSESASISVSESISMSMSGS
                     ISTSVSESVSTSTSESTLSSLSESASTSALKSTSVSEASNILETQASLTDKGRESFSA
                     NQIVTESSLVTDAGKNASVSSLIEITKPKSELQTSKMSNESLITPEKPQVMIASDKTG
                     NETLTPTIRLKSVIQPRSMNLIALSSEMDLIPLEEVSDTEMLGKDVSSEFQKVNIALK
                     DNTLSEPGTVKLDKSENLVLNFDFSIASVNEGDFFTVKLSDNLDTQGVGTTLKVQDII
                     DTSGQLLATGSYSPLTHNITYIWTKYASTLNNINAQVKLPVWPDQRKVSKTTSDKQRF
                     TATLNNQVASIEERVQYNSPSVTEHTNVKTNVRSRIMKIDDEKQTETYITQINPEGKE
                     MYFASGLGNLYTIIGSDGTSGSPVNLLNAEVKILKTNSKNLTDSMDQNYDSTEFEDVT
                     SQYSYTNDGSKITIDWKTNSISSSTSYVVLVKIPKQSGVLYSTVSDINQTNGSKYFYG
                     YTQVDGESDANAEKKFLSESASTSASTSASTSASASASTSASISASTSASISASTSAS
                     TSASTSASTSASMSTSTSVSTSASTSASMSASTSASTSASTSASTSASTSASTSASTS
                     ASTSASTSTSTSASTSASMSASTSASTSASTSASMSASTSASISASTSASMSASTSAS
                     TSASTSASTSASMSASTSASTSASTSASTSASMSASTSASTSASTSASTSASTSASTS
                     ASTSASTSSSTSASTSASTSASTSASTSASMSASTSSSTSASMSASTSASMSASTSAS
                     TSASTSASMSASTSASTSASMSASTSASMSASTSASTSASTSASMSASTSASTSASMS
                     ASTSASTSASMSASTSASTSASMSASTSASMSATTSASTSVSTSASTSASTSASTSSS
                     SSVTSNSSKEKVYSALPSTGDQDYSVTATALGLGLMTGATLLGRKKSKKDKD"
BASE COUNT         1196 a          742 c          709 g          963 t
ORIGIN      
        1 aaatcatact aaaaatcaat tgtaaggttt atatctgtat aaaaatgatg tgtctacgtg
       61 cttaacggta tcattatttt tgtgtttaat aagatagatt tctaatcact taattttact
      121 gttatgtttt taaaccaaaa caaaatataa aaaaatactt aaatatggag gaaacatgtc
      181 caaaaagact tttggcaagc agttaacagt tgtaggttct aagagtagag tcaagatgca
      241 taaatcagga aaaaactggg taagaacaat aatgtcgcat tttaatctat ttaaagcgat
      301 taaagggaga gcaactgttg aagcagatgt gtgtgttcaa aatattgaaa aagaagaccg
      361 actatcttca ggaaatttga cctatctcaa aggattacta gctgctggag ctctgctagg
      421 tggagcgagt ttaaccagtc gtgtttatgc agatgagact ccagttgttc aagaacaatc
      481 gagttctgta ccaacacttg cagaacaaac ggaagtgact gttaaaacaa ctgctgttca
      541 aaattatcaa gatgggacag tatcgaaaaa catcatcgac tccaatagtg tatctatgtc
      601 agagtcagcc tcaataagtg ttagtgaatc tatcagtatg tctatgtcag ggtcaatatc
      661 aacaagtgta agtgaatctg taagtacatc tacctcagaa agtactctat caagtctttc
      721 agaatcagct tcaacaagtg ctttaaaatc tacaagtgta agtgaggctt caaatattct
      781 tgaaactcag gcttctttaa ctgataaagg aagagagtcg ttttcggcaa accagatagt
      841 aacagaaagt agcttagtta ctgatgctgg taaaaatgct tcagtatcta gcctaattga
      901 aattacaaaa ccaaaatcgg agttacagac ttccaaaatg tcaaatgagt cgcttataac
      961 tccagagaaa ccccaagtaa tgattgcaag cgataaaact gggaatgaga ctctaactcc
     1021 gacaattaga ttaaaatcag ttattcagcc aaggagtatg aacttgatag ctttgagctc
     1081 ggagatggac ttgataccac tagaagaagt gtctgatact gaaatgttag gtaaagacgt
     1141 atcaagtgag tttcagaaag ttaatattgc gttaaaagat aacactctta gtgagcctgg
     1201 aacagttaaa ttagataaat cagaaaacct tgttttgaac tttgactttt caatcgcttc
     1261 tgttaacgag ggagatttct ttactgtaaa gctttctgat aatcttgata cacaaggggt
     1321 tggtactact ctaaaagttc aagatataat agatacaagt ggtcagttat tagcaacggg
     1381 atcatatagt cctttaacac ataatattac atacatctgg acaaaatatg cttctacatt
     1441 gaataatatt aatgctcaag tcaagctgcc agtttggcct gatcagagaa aagtttccaa
     1501 aacgacttca gataagcagc gctttactgc aacattgaac aatcaagttg cttcaattga
     1561 ggaacgtgtt cagtataata gtccttcagt gacagaacat actaatgtta agacaaatgt
     1621 aagatctcgg atcatgaaga ttgatgatga aaaacagaca gaaacttata ttactcaaat
     1681 taatcctgaa ggtaaggaaa tgtatttcgc atcaggactt gggaatctat atactattat
     1741 cggttcagat ggaacatcag gttcaccagt taatttatta aatgcggaag taaagattct
     1801 aaaaactaat tcaaaaaatc ttacagatag tatggatcaa aattatgatt cgactgagtt
     1861 tgaagatgtg acttcccagt atagttatac taacgatggt tctaaaatta ccatagattg
     1921 gaaaacaaat tctatttctt cctctacatc ttatgttgtt ttagtcaaaa tacctaaaca
     1981 aagtggtgta ttgtattcga ctgtttctga tataaatcaa acaaatggtt ctaaatattt
     2041 ttacggatat actcaagtag atggagaatc agatgcgaat gctgaaaaaa aatttttatc
     2101 cgaaagtgct tctacgtcag ctagtaccag tgcttcaact tcggcaagcg ctagcgcttc
     2161 cacgtcagca agtattagcg cttccacaag tgcaagtatt agcgcctcaa cgagtgcctc
     2221 cacgtcagcc agcacaagcg cttccacaag tgcaagtatg agcacctcaa cgagtgtatc
     2281 cacgtcagca agtactagcg cttcaatgag tgcatcgaca tcagcaagta ccagtgcttc
     2341 cacaagtgca agtacgagtg cctccacgtc agccagcact agcgcctcaa ccagtgccag
     2401 taccagtgcc tcgacgtcaa caagtactag cgcatccacg tcagcaagta tgagcgcatc
     2461 cacgtcagca agtaccagtg catccacgtc agcaagtatg agcgcctcca caagtgcaag
     2521 tatcagtgcc tccacgtcag caagtatgag cgcatccacg tcagcaagta ccagtgcctc
     2581 cacgtcagca agtactagcg cctcaatgag tgcatcgacg tcagccagca ccagtgcttc
     2641 cacaagtgca agtactagcg cctcaatgag tgcatcgaca tcagcaagta ccagcgcttc
     2701 aacaagtgca agtacgagtg cctccacgtc agcaagtact agcgcctcaa catcggcaag
     2761 tacaagttct tccacaagtg caagtaccag tgcatcgaca tcagccagca ctagcgcctc
     2821 aacgagtgcg tcaatgtcag ccagcacaag ttcttcaaca agtgcctcga tgtcagccag
     2881 cactagcgct tcaatgagtg catcgacgtc agctagcact agcgcctcaa cgagtgcgtc
     2941 aatgtcagcc agcacaagtg cttcaacaag tgcctcgatg tcagccagca ctagcgcttc
     3001 aatgagtgcg tcgacgtcag ccagcaccag cgcttcaacg agtgcgtcaa tgtcagccag
     3061 cacaagtgct tcaacaagtg cctcgatgtc agccagcact agcgcttcaa cgagtgcgtc
     3121 aatgtcagcc agcacaagtg cttcaacaag tgcctcgatg tcagccagca ctagcgcttc
     3181 aatgagtgcc acgacgtcag ccagcaccag cgtctcaacg agtgcatcga catcagcaag
     3241 taccagcgct tccacaagtt cttcaagctc agtgacttct aattcatcaa aagagaaggt
     3301 gtattctgcc ttaccttcta cgggtgacca agattattct gtaactgcta ctgccttagg
     3361 tttaggttta atgactggtg caaccctttt gggacgaaaa aaatctaaaa aagataaaga
     3421 ctaaacctac tttgaattct taaagtacta aatgacatgg taaaaggcat atctcttaat
     3481 atttttagag agatatgcct ttttgttgta gaagtttgta aaaagccttg taaatctata
     3541 tttatcaagt gacaagcaat gtttttctgc taaaatatga taaaattaag aagtggaatt
     3601 tatttctgaa
//
LOCUS       DATRNK010000109         3380 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.110, whole genome shotgun sequence.
ACCESSION   DATRNK010000109 DATRNK010000000
VERSION     DATRNK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.110"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(65..1213)
                     /gene="nagA"
                     /locus_tag="VBC52_001973"
     CDS             complement(65..1213)
                     /gene="nagA"
                     /locus_tag="VBC52_001973"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEO6883528.1"
                     /translation="MTKYIKADRFFYADHVKENGYLEIKDNHFGKWIENISGQEEILD
                     YSGYQIAPGLVDTHIHGFAGADVMDCDSEGILRMSAGLLSTGVTSFLPTTLTSDTKRL
                     EEASKSVAAVAGKEQGAKIQGIYFEGPYFTEEYKGAQNPIYMRNPNLEEFAQWQKAAK
                     GLITKIALAPEREGVEEFVSAITKQGVTVALGHSNGTYKEAKKAVKAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVDPVACDILMTQKGHNHVALITDCMAA
                     GGAPDGDYMLGELPVVVSNGTARLKDGGNLAGSILKLKDGLKNVVEWGIATPEEAVRM
                     ASYVPAVSVGIDNVCGQIKEGYAADFIVLDKHLDLVATYLDGEKRWQS"
     gene            complement(1491..2198)
                     /locus_tag="VBC52_001974"
     CDS             complement(1491..2198)
                     /locus_tag="VBC52_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEO6883529.1"
                     /translation="MKIDKKELLALIASIILLIFASVTFFLFKDHGTTQMDTVESSVN
                     HVSDSQLTEAQDMLDKFEKKPSEKLLKDVELALNKLSNSSKKEALQKRFKKAKDKYLK
                     DEADKKATKDATDLVEILEQAPSEENVLKAEAAVNKLTVKESKEALQKRIDTVKTQYG
                     LIGNQTPSSSVAETTEQGTANPASQDTSSYVNQNVAPTYEQPQANNTPVTPGVNNTVP
                     TPGTGTVPATNGTGVAQ"
     gene            2390..2701
                     /locus_tag="VBC52_001975"
     CDS             2390..2701
                     /locus_tag="VBC52_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEO6883530.1"
                     /translation="MIPSQMLKGLLEGAILKIIQQEETYAYEISKKLNSYGFGEISEG
                     TIYPIILRLQSNKYIKGQQKPSPQGPKRKYYTLTNEGVEQLAQFKQNWESLSKALNKL
                     L"
     gene            2722..>3380
                     /locus_tag="VBC52_001976"
     CDS             2722..>3380
                     /locus_tag="VBC52_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883531.1"
                     /translation="MDKLKKLQKENTLLEGRIDNSNNQTYTDMIVYLRGASISPYHQE
                     LIRNDIVNMLLEAQERQASLVSVFGEDRHDFINQVIKSTPKISKKEETLQRWDLAILL
                     LTIQMIIFLGGYLITEALQQSVPDLIPITLLDVLFAIFISIIAVKIADTIIYATYNFD
                     KSKEKKYFFRYIFLILSLIIAYILIGKYYHLPFINIPLWIYLIILGLSFSLHIIVKKY
                     L"
BASE COUNT         1083 a          679 c          496 g         1122 t
ORIGIN      
        1 tattgtcaaa ctatatttca ttaaaaatta ataaattaaa aagaactagt aaaaactagt
       61 tctttcaaga ttgccaacgt ttttccccat caaggtatgt tgcaactaaa tctagatgtt
      121 tatctaatac aataaaatcc gctgcataac cttcttttat ctgtccacag acattatcaa
      181 tgcctacaga cactgccgga acataactcg ccatacgcac tgcctcttcc ggagtagcaa
      241 ttccccattc tacaacattt tttaatccat ctttgagctt aagaatagaa ccagctaaat
      301 tacctccatc tttcaaacgc gcagttccat tagacacaac cacgggcaat tcccctaaca
      361 tataatctcc atcaggggca cctccagctg ccatacaatc tgttattaat gccacatgat
      421 tatggccttt ttgtgtcatt aaaatgtcac aagcaacagg atcaacgtga tgaccatcgc
      481 aaatgagttc ggcataggta ttaggtaaat tataaaccgc accaaccata cctggttctc
      541 ggtgtgtcaa accacgcata ccattatacg catgtaccca aactgaggca cccgccttca
      601 ctgccttttt agcttcttta tatgtcccat tagaatgacc taaagctacc gtaacacctt
      661 gctttgtgat tgctgagaca aattcctcaa ctccctcacg ttctggtgcc aaagcaattt
      721 ttgtaatcaa gccttttgca gccttctgcc attgagcaaa ctcttccaag tttggatttc
      781 tcatataaat cggattttga gctcctttat attcctcagt aaaatatggt ccctcaaaat
      841 agatgccttg aatttttgct ccttgttctt tgcccgctac tgccgcaact gatttagacg
      901 cctcttctag tcgctttgta tctgaagtta gggttgtcgg caagaaagat gtgactccag
      961 tagatagtaa gccagcactc attcgcaaaa ttccttctga atcacaatcc ataacatcag
     1021 cgccagcaaa accatgaata tgtgtatcaa ctaaaccagg tgctatttga taacctgaat
     1081 aatctaaaat ctcctcttgt ccagaaatat tttctatcca tttaccaaag tgattatctt
     1141 ttatttctaa ataaccgttt tcctttacat gatcagcata aaagaatcgg tcagccttga
     1201 tgtacttcgt catcttcttc cccccattca ctctcattat aacgctttaa ggtatttttg
     1261 taaatggtat atacctattt tttgacaagt catggtttat tctaagaaag aactgtacac
     1321 ttgttataaa ccacttcact tggttgtatt tttaaggttt taattgctgt tgctcataat
     1381 tcttttcctt cttcaaaact atcaattgct aattctctta tagtttttga tgagaatggc
     1441 taattaaaaa gcatagcaaa aaagtctagt aaaactagac tttcaggatt ttactgagca
     1501 acacctgtcc cattagtagc aggtacagta ccagttcctg gagtcggaac agtattgtta
     1561 acccctggag taactggtgt attattcgct tgcggttgct cataagttgg tgctacattc
     1621 tgattaacgt aactagaagt atcctgacta gcaggattag ctgtcccttg ttctgtagtt
     1681 tcagctactg aactagaggg agtttgattc ccaatcaaac catattgtgt cttaacagta
     1741 tcaatacgtt tttgtaaagc ttctttagat tctttgacag ttaacttatt gactgctgct
     1801 tcagctttta acacattttc ttctgaaggg gcttgttcta gaatttctac taaatctgta
     1861 gcatctttgg tagctttctt atctgcttcg tctttaagat acttatcttt tgctttctta
     1921 aaacgttttt gcaaagcttc ttttttagaa gaattggaca atttatttaa agcaagttcg
     1981 acatccttca ataatttttc tgaaggtttc ttctcaaatt tatctaacat atcttgtgcc
     2041 tcagttaatt gagaatcact gacatggtta acactgcttt ctacagtatc catttgagta
     2101 gtgccatgat ctttaaataa gaaaaacgta acagaagcaa aaattaataa aataatacta
     2161 gcaataagtg ctaaaagttc ttttttatct attttcatac tcactcctaa agtttcttca
     2221 cattattata acataaacta aaggacataa acaaacctat tttagaagaa tcatcgtcta
     2281 tcactcttat tttctaccgg taacttagtt atatttgaca tctcaactac tttgtattac
     2341 aatgtagcta taccagtaac ttgtattaca atatagacaa ggatataata tgattccatc
     2401 gcaaatgcta aaaggattac ttgaaggagc tatcctaaaa atcatacagc aagaggagac
     2461 ttatgcttat gaaatctcaa aaaaattaaa ttcttatggt tttggagaaa tttccgaagg
     2521 gacaatctac cccattatct taaggctaca aagcaataaa tatataaaag gacaacaaaa
     2581 gccttcccct caaggaccta agcgtaaata ttatacgtta accaatgaag gcgtggagca
     2641 attagcccaa tttaaacaga attgggagtc acttagtaaa gcattaaaca aactactcta
     2701 aaaattggag aggaaactag catggataaa ctgaaaaaat tacaaaaaga aaatacgcta
     2761 ttagaaggaa gaattgataa ctctaataac caaacctata cagatatgat tgtctatttg
     2821 cgaggtgcta gcattagccc ctaccatcag gagctcattc gaaatgatat tgtcaatatg
     2881 ttattagaag cacaagagcg acaagcaagt ttagttagtg tatttggaga agataggcat
     2941 gactttataa accaagttat taaatctaca cctaaaatct ctaagaaaga agaaacatta
     3001 caacgttggg atttagccat tttactacta actatccaaa tgattatctt tttaggagga
     3061 tatctcatta ctgaagcact tcaacagtca gtaccagatc taatccctat tactctatta
     3121 gatgttcttt ttgcaatctt tatcagtatt attgctgtca agatagctga taccataata
     3181 tatgctacat ataacttcga taaatcgaaa gaaaagaaat atttctttcg atatatattt
     3241 ctaattcttt cgcttatcat agcttatatc cttataggaa aatattatca tcttcctttc
     3301 attaatatcc ccctttggat ttatttaatt atactgggac ttagcttctc tcttcatatc
     3361 attgttaaaa aatacctgaa
//
LOCUS       DATRNK010000110         3164 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.111, whole genome shotgun sequence.
ACCESSION   DATRNK010000110 DATRNK010000000
VERSION     DATRNK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.111"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            96..554
                     /locus_tag="VBC52_001977"
     CDS             96..554
                     /locus_tag="VBC52_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883532.1"
                     /translation="MRKYIKWLIPISIFGMILGGCQMNSEHKSQSNEVKNSKQSEVKK
                     DKKMTKKEQLAYLKEHEQEIIDYVKLHNNQIESVQFDWSSVKVEQSGNGTPQGGDYNL
                     SLRGKFNHLQNSKLIVDFYLAHKNDIPNIKSMGMLNKPYIHKNGIWHIYE"
     gene            618..2252
                     /locus_tag="VBC52_001978"
     CDS             618..2252
                     /locus_tag="VBC52_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6883533.1"
                     /translation="MSNWNLDNFDNLHSSLAESAYNSRPNSFPELFETDSVTEVKFSQ
                     PSEDNKGQITQGGTNLPNDGIVYLQPDKSLKSIDENVKVLIPDVNGGYHTEHYVTHSY
                     QKGVLTDDKAGFNAYYLSDTEKIDSTTKHTYLAIRGSDGIGLDTLNDWVSNNAMFAVS
                     NKYIPQAKLANKAMKEKIAELKGKAPGAIMDVTGHSLGTIVSSQAVVNLSYAELENVG
                     QVVLFDGPDVSRSLEKMEGISAKKIQEAGKHVTYYVNPFDIVSMLNREKPWKDQFGKV
                     NVIVPSKYTGTFDEQSSHDFGAFQIDSSGNPLVASESYHPELLTSGQDLARLEKKFIK
                     NLLGEHYDSDKLGPVLKGALTGDLTRLIATLKACGISVTVNEAKKLYDNFQKDYKAII
                     SKAKKDGLEWDRKNIASFHSRIKSASGSQRILLRTQMLYMATQLAEADVSEKVSATKK
                     HISEAKDTIVEISTSTISSAEIMAMHLDQSEVDALVSDIKMSTVWNDGVETSDYEALD
                     HYKTKMTTFTTNLVTVAQNLTAQDEQLAGDIVTNLS"
     gene            2271..2543
                     /locus_tag="VBC52_001979"
     CDS             2271..2543
                     /locus_tag="VBC52_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883534.1"
                     /translation="MSKELKLPLNEMLKEQERIAIKAIVATAKSTITDTGTNLKATLE
                     SDAQDLKNYASTYNANITDGLEGQSAQAASDFLTQLPKPELDNPVN"
     gene            2553..2903
                     /locus_tag="VBC52_001980"
     CDS             2553..2903
                     /locus_tag="VBC52_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883535.1"
                     /translation="MIDTKKLQELDQEYDQNLRNIYRNREQLEDDFHLFMARTDSLKE
                     SVYQATLGQGWELPQEAHAHLYNMDDNKDTFISEFNEYMEKLEEKEIDLRRVYNDRVD
                     ELYQKAKQNEAKKG"
BASE COUNT         1202 a          523 c          577 g          862 t
ORIGIN      
        1 ttatactaaa attaatttta taatgttggt gaataatagt taatttcatt atatacttga
       61 tatataaaat ttatattcta agaaaagaga acatgatgag aaaatatata aaatggttaa
      121 ttcccatatc aatttttggg atgatacttg gaggctgtca aatgaatagt gaacataaaa
      181 gtcagtcaaa cgaagtaaaa aatagcaagc aatcagaagt gaagaaagat aaaaaaatga
      241 caaaaaaaga acaattagcc tatctcaaag agcatgagca agaaatcata gattatgtaa
      301 aattacataa caaccaaatt gagtccgttc aattcgattg gtcaagtgta aaagtagaac
      361 aaagcgggaa tggaactcca caagggggtg attataatct ttcactgaga ggaaagttta
      421 atcatctaca aaattcaaaa ttaatagttg atttttattt agctcataaa aatgatatcc
      481 caaatatcaa atcaatggga atgctaaata agccgtatat acataaaaat ggtatttggc
      541 acatttatga atagcttgaa tcatcctata taaagtaata gaatttttac cacaagcaag
      601 gtagaggaga agaataattg agtaattgga acttagataa ttttgataac ttacatagct
      661 ctttagcaga aagtgcatat aatagtcgac ctaattcatt tcctgaatta tttgagactg
      721 actcagtaac agaagtaaaa ttttctcaac cctcagaaga taacaaaggt caaatcacac
      781 agggtggcac taacctcccc aatgatggta ttgtctacct gcaaccagat aagtccttaa
      841 aatctattga tgaaaatgta aaggtcctca ttccagatgt caatggaggc tatcacactg
      901 agcattatgt tactcattct tatcaaaaag gtgtgcttac tgacgataag gcaggtttta
      961 atgcttacta cctgtcagat actgaaaaaa tagatagcac tacaaagcac acttatctag
     1021 ccattcgtgg tagtgatggg attggtctag atacactaaa cgattgggtt agtaacaatg
     1081 caatgtttgc tgtctctaac aagtatattc cacaagcgaa attggctaat aaggctatga
     1141 aagaaaaaat tgccgagcta aaaggaaaag cgcctggagc tattatggat gttactggtc
     1201 actcactcgg tacgattgta tcttctcaag cagttgttaa tcttagctat gcagaattgg
     1261 agaatgttgg tcaagtagtc cttttcgatg gccctgatgt atcaagaagc ttggaaaaaa
     1321 tggaaggcat ttctgctaaa aaaatccaag aagctgggaa gcatgttacc tactatgtca
     1381 atccctttga tatagttagt atgcttaatc gtgaaaagcc atggaaagac caattcggga
     1441 aagtaaatgt cattgttcct agtaaatata cgggtacttt tgatgagcaa tcttctcacg
     1501 attttggtgc cttccaaatt gatagtagcg gaaatccttt agttgcttct gaaagctatc
     1561 atccagaatt attaacatct ggtcaagact tagctagact cgaaaaaaaa ttcataaaga
     1621 atcttttagg agagcattat gattcggata agctaggacc agtactcaaa ggcgctctga
     1681 ctggggacct gactcgacta attgcaacat taaaagcttg tggtatcagt gtcactgtaa
     1741 acgaagctaa aaaactgtac gataacttcc aaaaagacta caaagccata atttccaaag
     1801 ctaaaaaaga tggcctcgag tgggatcgca aaaacatcgc aagcttccat agtaggatca
     1861 aatcagcatc aggcagtcaa cggattttac ttagaactca aatgctctat atggcaacgc
     1921 aattagcaga agcagatgtt tcagaaaaag tctctgcaac aaaaaaacat attagtgaag
     1981 ctaaggatac gattgtagaa atatcgacat ccactatttc atcagctgaa ataatggcta
     2041 tgcaccttga ccaatcagaa gttgatgctc tggtttcaga tataaaaatg tcaacagttt
     2101 ggaatgatgg cgtagagact tctgattatg aagcacttga tcactataaa acaaaaatga
     2161 ccacatttac gaccaatttg gtaacagttg cacaaaattt aacagctcaa gatgagcaac
     2221 tagctggtga tattgtcacg aacttatcct aaaatagaga gattaaatga atgtccaaag
     2281 aattgaaatt gcccttgaat gaaatgttga aagaacaaga aagaatagct attaaggcaa
     2341 tagttgctac agcaaaaagc actattacgg atactggaac aaatttaaaa gcaactttag
     2401 agtcagatgc ccaagaccta aaaaactatg catccaccta taacgccaat atcacggatg
     2461 gtcttgaagg ccagtcagct caagcagcat ctgatttctt gacacaactt ccaaaaccag
     2521 agttagataa tccagtcaat taggaggcta gtatgataga tacaaaaaaa ctgcaagaac
     2581 tcgatcaaga atatgatcaa aatctcagaa acatttatcg taacagagag caactagaag
     2641 atgactttca cttgtttatg gcaagaacag acagcctaaa agagtctgtc tatcaagcaa
     2701 ctttaggaca aggttgggaa ttaccccaag aagctcatgc acacctatat aatatggatg
     2761 ataataagga tacattcata tcagaattca atgaatatat ggaaaagctt gaagaaaagg
     2821 aaatagatct tcgaagagtt tataatgata gagtagatga attgtatcag aaggctaagc
     2881 aaaacgaagc aaaaaaagga taaaaatttt ttcaagtaat atatataaat atgaaaaata
     2941 aagaatcttg cagtaaaatg agtatttatt tttcacgacg aacagtcact acaaaatcac
     3001 taaaatctca aaattacttg gtttttaagt taatgtctaa tactcaaacc ttgttaaatc
     3061 aatgtttgtt atctttaaaa agtgctcagt agcactcaaa atccattgtc ctcacggaca
     3121 tgccggttcg accccggccg ccggtatact tataaagaca aggt
//
LOCUS       DATRNK010000111         3068 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.112, whole genome shotgun sequence.
ACCESSION   DATRNK010000111 DATRNK010000000
VERSION     DATRNK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.112"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(133..2124)
                     /gene="uvrB"
                     /locus_tag="VBC52_001981"
     CDS             complement(133..2124)
                     /gene="uvrB"
                     /locus_tag="VBC52_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEO6883536.1"
                     /translation="MIDRKDTNRFKLVSKYSPSGDQPQAIETLVDNIEGGEKAQILKG
                     ATGTGKTYTMSQVIAQVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGQEISRDQLLNNLVDIQFERNDIDFQRGKFRVRGDVVEVFPASRDEHAFR
                     IEFFGDEIDRIREIESLTGRVLGEVEHLAIFPATHFMTNDEHMEEAISKIQAEMENQV
                     ELFEKEGKLIEAQRIRQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPFTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYEMGQTDTVVEQIIRPTGLLDPEVEVRPSMGQMDDLLGEINLRTEKG
                     ERTFITTLTKRMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSNGHVIMYADKITDSMQ
                     RAMDETARRRRLQMDYNEKHGIVPQTIKKEIRDLIAITKSNDSDKPEKVVDYSSLSKK
                     ERQAEIKALQKQMQEAAELLDFELAAQIRDVILKLKAID"
     gene            complement(2185..2901)
                     /locus_tag="VBC52_001982"
     CDS             complement(2185..2901)
                     /locus_tag="VBC52_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002961501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6883537.1"
                     /translation="MYGELGTFSFSAFLLLIWVKFVEKRPFSELGFSTKFKRTLWSLI
                     KGWSIGFILFSISVITAYILGGLDFHSYDVSKATIFYVVTLLPFWLIQSGTEELLTRG
                     WLLPLINHRFHLAVAIGVSSTLFGILHLVNAHVTFLSIVSIICSGVLMSLYMIKSGNI
                     WSVAALHGAWNFSQGNLYGIAVSGQKAGASLLHFTVKENAPDWISGGAFGIEGSLISI
                     FVLLAAIIYLLWLIKTEETD"
BASE COUNT         1010 a          637 c          464 g          957 t
ORIGIN      
        1 aagaaaaata tcgaaaaatc tttgataaaa tataacatcc acctactgta ttcttttgat
       61 ttcaattatt aataaaacga ctagagcact tcattaacta ctctagtcgt tttattaatt
      121 ctgttcatcg tattaatcga ttgcttttag ttttaagata acatctcgga tttgtgcagc
      181 tagctcaaag tccaataatt cagctgcttc ttgcatttgc ttttgaagag ctttaatttc
      241 agcttgcctt tctttcttag ataaactact gtaatcgaca actttttcag gtttatcgct
      301 gtcattactt ttagtaatag caattaaatc acggatttct tttttaattg tttgtggcac
      361 aattccatgc ttttcattat aatccatttg taaacgacga cggcgagcgg tttcatccat
      421 agctctttgc atagaatctg ttattttatc agcatacata ataacatgac cattactgtt
      481 acgtgctgca cgtccgattg tttggataag accacgctca ttacgaagga aaccttcctt
      541 atcagcatct aagattgcaa caagggaaac ctcaggaaca tctattcctt cacgtaagag
      601 attaatacca attaaaacat caaacacacc aagtcgtaaa tcacgaataa tctctgtacg
      661 ttctaaggtt ttaatatcgc tgtgcatata cttaacttta actcccattt ctttgagata
      721 atctgttaag tcttctgcca tgcgctttgt caatgttgta ataaaggtac gctcaccttt
      781 ttcagtcctc aaattgattt caccaagcaa gtcatccatc tgccccatac ttggtctaac
      841 ttcaacttct ggatcaagaa gtcctgttgg ccggataatt tgctcaacaa ccgtatctgt
      901 ttgccccatt tcatagtctc ctggcgttgc agatacatag acaatttgat ggacatggct
      961 ttcgaattct tcacgacgaa gtggacggtt atctaaggca gatggtaaac gaaaaccgta
     1021 attaaccaac atttctttac gcgaacggtc tccattatac atccctttga tttgccccat
     1081 tgtcatgtga ctctcatcaa tcataatcaa aaaatcttct gggaagaaat ctaacaaagt
     1141 aaaaggtggc tcaccttcac ttctaccatc catgtgacgt gagtagttct caacaccatt
     1201 tgtatatccc atttcacgaa gcatttcaat atcatattct gtcctttgtc gaatacgttg
     1261 cgcttcaatc aatttccctt ctttttcaaa aagttctact tgattttcca tttcagcttg
     1321 tatttttgaa atagcttctt ccatatgttc atcatttgtc ataaagtgtg ttgctgggaa
     1381 aatagctaaa tgttccactt ctcctagcac acgccctgtt aacgattcta tttctcttat
     1441 acgatcaatt tcatcaccaa aaaattcaat tcgaaaggca tgctcatcac gactcgcagg
     1501 aaaaacttct acaacatcgc cacgaacgcg aaattttcca cgttgaaaat caatatcgtt
     1561 acgctcaaat tgaatatcaa cgagattgtt caataactga tcacgtgaaa tttcttgacc
     1621 tggcctaagg ctaacaacac tgtctgcata ctctttagga gaacctaaac cgtaaataca
     1681 agaaacagat gctacaacaa taacgtcatt tctttctaat aacgaagaag tcgctgagtg
     1741 acgtaatttg tcaatttcgt cattgactga actatctttt tcaatataag tatccgaaga
     1801 aggcacataa gcttctggtt gataataatc ataatatgaa acaaagtact ctacggcatt
     1861 gtcaggaaaa aattctttaa actctccata aagttgacct gctaaggttt tattatgtgc
     1921 aatcaccaag gttggcttgt taacttgggc gataacttgg ctcattgtgt aggttttacc
     1981 agtaccagta gcacctttta aaatctgagc tttttctcct ccttcaatat tgtcaaccaa
     2041 cgtttcaata gcttgaggtt gatctcccga agggctatat ttggaaacta atttaaatcg
     2101 gttagtatct tttctatcta tcatttcttt acctcgatta ctttatttta acatagaaaa
     2161 aagaaaaaga ctgagctgca acactcaatc tgtttcttca gtcttaatta gccatagaag
     2221 gtatataatt gctgctaaga gaacaaaaat agaaatcaaa cttccttcta ttccgaatgc
     2281 accacctgat atccagtccg gtgcgttttc tttaacggta aaatgcaaaa gagatgctcc
     2341 tgctttctga ccacttacgg caataccata gagattacct tggctaaaat tccaagctcc
     2401 atgtaaggct gcaacactcc aaatattacc acttttaatc atatacaagg acattaaaac
     2461 tcccgaacat ataatactta caatagaaag aaaagtgaca tgggcattta ccaagtgcaa
     2521 aatgccaaat aaagtggatg agacccctat ggcaacagct aaatgaaaac gatgattaat
     2581 tagaggaaga agccaccctc gtgttagcaa ttcttccgta ccgctttgaa taagccaaaa
     2641 aggaaggaga gttacaacat agaaaatcgt tgccttagaa acatcatagc tatgaaagtc
     2701 aagtcctccc aatatataag cagtgatgac agaaattgaa aatagaataa aacctatact
     2761 ccaacctttt ataagtgacc acagagttct tttaaacttt gtagaaaacc ccaattctga
     2821 aaaaggtctt ttttctacga attttaccca gataagcaaa aggaatgctg aaaaactaaa
     2881 ggttcctaat tctccataga tattattaaa taaattaata aactcaaaaa agtataggaa
     2941 gaaaacttat agagtacaga agacactaga acaattccca caccacctag agttagacca
     3001 acgaaaaaaa ataggtatac cataaaacaa gcaaataaca ataatagcca tgacggccag
     3061 tttttata
//
LOCUS       DATRNK010000112         3045 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.113, whole genome shotgun sequence.
ACCESSION   DATRNK010000112 DATRNK010000000
VERSION     DATRNK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.113"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..785
                     /locus_tag="VBC52_001983"
     rRNA            <1..785
                     /locus_tag="VBC52_001983"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            840..912
                     /locus_tag="VBC52_001984"
     tRNA            840..912
                     /locus_tag="VBC52_001984"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:873..875,aa:Ala,seq:tgc)
     gene            1067..>3045
                     /locus_tag="VBC52_001985"
     rRNA            1067..>3045
                     /locus_tag="VBC52_001985"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          878 a          624 c          871 g          672 t
ORIGIN      
        1 aggctcgaaa gcgtggggag caaacaggat tagataccct ggtagtccac gccgtaaacg
       61 atgagtgcta ggtgttaggc cctttccggg gcttagtgcc gcagctaacg cattaagcac
      121 tccgcctggg gagtacgacc gcaaggttga aactcaaagg aattgacggg ggcccgcaca
      181 agcggtggag catgtggttt aattcgaagc aacgcgaaga accttaccag gtcttgacat
      241 ccttctgacc ggcctagaga taggctttct cttcggagca gaagtgacag gtggtgcatg
      301 gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaaccccta
      361 ttgttagttg ccatcattaa gttgggcact ctagcgagac tgccggtaat aaaccggagg
      421 aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc tgggctacac acgtgctaca
      481 atggttggta caacgagtcg caagccggtg acggcaagct aatctcttaa agccaatctc
      541 agttcggatt gtaggctgca actcgcctac atgaagtcgg aatcgctagt aatcgcggat
      601 cagcacgccg cggtgaatac gttcccgggc cttgtacaca ccgcccgtca caccacgaga
      661 gtttgtaaca cccgaagtcg gtgaggtaac cttttaggag ccagccgcct aaggtgggat
      721 agatgattgg ggtgaagtcg taacaaggta gccgtatcgg aaggtgcggc tggatcacct
      781 cctttctaag gataaggaaa cctgccattt gcgtcttgtt tagttttgag aggtcttgtg
      841 gggccttagc tcagctggga gagcgcctgc tttgcacgca ggaggtcagc ggttcgatcc
      901 cgctaggctc cattgaatcg aaaggttcaa attgttcatt gaaaattgaa tatctatatc
      961 aaattccacg atctagaaat agattgtaga aagcaacaag aaaataaacc gaaaacgctg
     1021 tgaatattta atgagttttc tagttttaaa gaaactaggt taataaggtt aagttaataa
     1081 gggcgcacgg tggatgcctt ggcactagaa gccgaagaag gacgtgacta acgacgaaat
     1141 gctttgggga gctgtaagta agcgctgatc cagagatgtc cgaatggggg aacccactag
     1201 ttaatgacta gtatccatat ctgttaagga tatgagaagg aagacgcagt gaactgaaac
     1261 atctaagtag ctgcaggaag agaaagcaaa agcgattgcc ttagtagcgg cgagcgaaac
     1321 ggcaggaggg caaaccgaag tgtttacact tcggggttgt aggactgcga tataggatta
     1381 atcattatag aagaattacc tgggaaggta agccaaagag agtaacagcc tcgtatttaa
     1441 aatgatagat taaccttagc agtatcctga gtacggcgag acacgcgaaa tctcgtcgga
     1501 atctgggagg accatctccc aaccctaaat actctctagt gaccgatagt gaaccagtac
     1561 cgtgagggaa aggtgaaaag caccccggga ggggagtgaa atagaacctg aaaccgtgtg
     1621 cctacaacaa gttcgagccc gttaatgggt gagagcgtgc cttttgtaga atgaaccggc
     1681 gagttacgat atgatgcgag gttaagttga agagacggag ccgtagggaa accgagtctt
     1741 aatagggcgt catagtatca tgttgtagac ccgaaaccat gtgacctacc catgagcagg
     1801 ttgaaggtga ggtaaaactc actggaggac cgaaccaggg cacgttgaaa agtgcttgga
     1861 tgacttgtgg gtagcggaga aattccaaac gaacttggag atagctggtt ctctccgaaa
     1921 tagctttagg gctagcgtcg atgttaagtc tcttggaggt agagcactgt ttgggtgagg
     1981 ggtccatccc ggattaccaa tctcagataa actccgaatg ccaacgagat ataatcggca
     2041 gtcagactgc gagtgctaag atccgtagtc gaaagggaaa cagcccagac caccagctaa
     2101 ggtcccaaaa tatatgttaa gtggaaaagg atgtggggtt gcacagacaa ctaggatgtt
     2161 agcttagaag cagctattca ttcaaagagt gcgtaatagc tcactagtcg agtgaccctg
     2221 cgccgaaaat gtaccggggc taaaacatat taccgaagct gtggatgaca caaaagtgtc
     2281 atggtaggag agcgttctat gtgtgaagaa ggtgtaccgt gaggagcgct ggaacgcata
     2341 gaagtgagaa tgccggtatg agtagcgcaa gacaggtgag aatcctgtcc accgtaagac
     2401 taaggtttcc aggggaaggc tcgtccgccc tgggttagtc gggacctaag gagagaccga
     2461 aaggtgtatc cgatggacaa caggttgata ttcctgtact agagtatata gtgatggagg
     2521 gacgcagtag gctaactaaa ccagacgatt ggaagtgtct ggtcaaacag tgaggtgtga
     2581 tatgagtcaa atgcttatat ctttaacatt gagctgtgac gaggagcgaa gtttagtagc
     2641 gaagttagtg atgtcacact gccaagaaaa gcttctagcg atacatatac tctacccgta
     2701 ccgcaaaccg acacaggtag tcgaggcgag tagcctcagg tgagcgagag aactctcgtt
     2761 aaggaactcg gcaaaatggc cccgtaactt cgggagaagg ggcgctgatt ttagatcagc
     2821 cgcagtgaat aggcccaagc aactgtttat caaaaacaca gctctctgct aaatcgtaag
     2881 atgatgtata gggggtgacg cctgcccggt gctggaaggt taagaggagt gcttagcgta
     2941 agcgaaggta tgaattgaag ccccagtaaa cggcggccgt aactataacg gtcctaaggt
     3001 agcgaaattc cttgtcgggt aagttccgac ccgcacgaaa ggcgt
//
LOCUS       DATRNK010000113         2995 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.114, whole genome shotgun sequence.
ACCESSION   DATRNK010000113 DATRNK010000000
VERSION     DATRNK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.114"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            232..522
                     /locus_tag="VBC52_001986"
     CDS             232..522
                     /locus_tag="VBC52_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEO6883538.1"
                     /translation="MSQIKLTPEELRISAQKYTTGSQSITDVLTVLTQEQAVIDENWD
                     GTAFDSFEAQFNELSPKITQFAQLLEDINQQLLKVADVVEQTDSDIASQINK"
     gene            624..1031
                     /locus_tag="VBC52_001987"
     CDS             624..1031
                     /locus_tag="VBC52_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883539.1"
                     /translation="MKKSTQIILLIVALFILVFSGGFYMKEQQRKEELKRNREYEVSL
                     VKALKNSYENIEEIKITHPVSTEIPGDWHCTVKISFNDKKSIVYNITHNLESKKNYSG
                     KFNEKNMNFFDSRIGKTKKTIKIIFSDGQEKIQ"
     gene            1031..1590
                     /locus_tag="VBC52_001988"
                     /pseudo
     CDS             1031..1590
                     /locus_tag="VBC52_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865562.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1563..2243
                     /locus_tag="VBC52_001989"
     CDS             1563..2243
                     /locus_tag="VBC52_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883540.1"
                     /translation="MAKHPEYFVNFRHKEDNVTWWNDFNKLDDKDYGTVKWVNGKSHK
                     IESWKFTDDGKLKDEKGNIVNPKSPAVQSVLYEEVHFQKAKAKLKKSGGKLSHSEKVY
                     LDSEQAIFIANGLTTASQTASDDIKKNAELVKEKASELFAKTKVMPPGITDLSPEELA
                     DTYSEGGVREDTIVTPIETFFDEKVTNAQEITTSYINLQKQIESGVQKLLEEDSKLAG
                     EFKEWSQY"
     gene            2228..2614
                     /locus_tag="VBC52_001990"
     CDS             2228..2614
                     /locus_tag="VBC52_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883541.1"
                     /translation="MVTVLDQNENKINSLKINLGKLEDNYHRENMEIEDKLYSLEDKK
                     NELETYLQEVYEETSHLLRQNDSDGSTFMSLNRIIESYQMELIDEYENYKQTIIVQEE
                     NSRTQFLKDRVKLENDISSLEGRKYY"
     gene            2648..2929
                     /locus_tag="VBC52_001991"
     CDS             2648..2929
                     /locus_tag="VBC52_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883542.1"
                     /translation="MSLIGAVLKGTKEDKEFKKQIQRALVRIVYAPEIVEEADSIKMT
                     YSDKIDNVSETAKSSIKTIANQIDSGLVGQFGEKVKAQVDLNKGSYDDL"
BASE COUNT         1204 a          394 c          529 g          868 t
ORIGIN      
        1 cacaattctc aaaatgttaa tcagtacgat tatatcaacg tttcaaagca ctttgcaagt
       61 cttgcaaggt gttttttaaa accttttgat aaccctttct gttttgaaag taaggtaaat
      121 gtaatataaa tcgctgtttg tatttttaat agattaacct attatgagtt ttatctgtgt
      181 aaggaacatc taaatatgct atactgcagt taaaaaaata gggagcaaat gatgtcacaa
      241 attaaactaa cacctgaaga actccgtatt tcagcacaaa aatatacaac aggatcacaa
      301 tcaattacag atgtgttaac agttttgact caagaacaag ctgttattga tgaaaattgg
      361 gatggtacag catttgatag ctttgaagcc caattcaatg aattatctcc aaaaatcaca
      421 caatttgcac aattattaga agatataaat caacaattat tgaaagttgc ggatgttgtc
      481 gaacaaacag actcagatat tgcctcacaa attaataaat aattggctta ttcaaattca
      541 taagtttaac ttaggaaggc ataagctctc ctaagttgat atattatagc tagatgatgt
      601 aattgtttca taaaggagta aaaatgaaaa agagtaccca aataatacta ctaatagttg
      661 cattattcat acttgttttt agcggaggat tttatatgaa agaacaacaa agaaaagaag
      721 aactaaaacg gaatcgagaa tatgaagtta gtctagtcaa agcattgaaa aattcctatg
      781 agaatataga agaaataaaa atcacacatc ctgtttcaac tgaaattcct ggagattggc
      841 attgtactgt aaagatttca tttaatgata aaaaatctat tgtttataat attacacata
      901 atttggaatc gaaaaaaaat tatagcggaa aatttaatga aaaaaatatg aatttttttg
      961 attcaagaat tggtaaaaca aaaaaaacta taaaaattat tttttcagat ggtcaggaga
     1021 agatacaata atggtattta catataccga gaaagaactt cgtgaattca atattggaga
     1081 taatgtatat tcagttaacc ccgattatgc agaaaaaaat cattcaactg tcataacgga
     1141 tttaccccag aaagataacg aaacaaatat tattacaaca gaagatagaa aaaatttaag
     1201 gtattaaaaa cttctcctga cgatatgtct ggctatcagg gtatggcagt tgcaccaatt
     1261 atcaagggta aagtagatta taacagtgtt gcagttatat ccgccgcaac ggattcaagt
     1321 aattataaag atttaattgg cgcagtttca tctgctcaac cacatcaaag ttcgactcag
     1381 ttaaaatcag ctgataaatt tttaaaagac gtacagtcac atgacaaatg gactgtgact
     1441 cagctttcag gctattctca atctgcatat atgttaaaat taggtgctca atatcatatc
     1501 ccgactacag tatttaatgg atggtttagg tatagcactt taaatgaaga tgaaaaaaat
     1561 tcatggctaa gcatcctgaa tattttgtga attttcgaca taaagaggat aatgtaactt
     1621 ggtggaacga ttttaataaa ctagatgata aagattatgg taccgtgaaa tgggtaaatg
     1681 gtaaaagtca taaaatagag agttggaaat ttactgatga tggtaagtta aaagatgaaa
     1741 aaggtaacat cgtaaatcct aagtcaccag ctgttcagtc tgttctttat gaagaagttc
     1801 attttcaaaa agctaaagct aaattaaaaa aatctggagg caaacttagt catagtgaaa
     1861 aagtatattt agattctgaa caggctatat ttattgctaa cggtttaacc acggcatctc
     1921 aaacagcttc tgatgatatc aagaagaatg ctgaattagt taaagaaaaa gcatcagaac
     1981 tatttgcaaa aactaaagtg atgcctcctg gtataacaga tttaagtccg gaagaattag
     2041 ctgatacata tagtgagggc ggagttagag aggacaccat agtaacacca attgaaactt
     2101 tctttgatga aaaagttact aatgcacaag agattacaac ttcatatatt aatcttcaaa
     2161 aacaaattga atctggtgtc caaaaattgc tagaagagga ctccaaatta gcaggagaat
     2221 ttaaggaatg gtcacagtac tagatcaaaa tgaaaacaaa attaatagtc taaaaataaa
     2281 cctagggaaa ttggaagata attatcatcg cgaaaatatg gagatagaag ataaattata
     2341 tagccttgag gataaaaaga acgagttaga gacttattta caagaagtct atgaagagac
     2401 aagtcacctt ttgagacaaa atgattcaga tggttcgact tttatgagtt taaatcgaat
     2461 aattgaaagt tatcaaatgg aattaattga tgaatatgaa aactataaac agactatcat
     2521 tgtccaagag gaaaatagtc ggacacagtt tctaaaagac cgagtaaaat tagaaaatga
     2581 catttctagt ttagaaggaa gaaaatacta ctaattcaga tggactataa agtgaaagag
     2641 gcaataaatg agtttaatag gcgctgtatt gaaaggaacc aaagaagaca aagaattcaa
     2701 aaagcaaatt caaagagctt tagtaaggat tgtctatgcc cctgaaatag tagaagaagc
     2761 tgattctatt aagatgactt atagtgacaa gattgataat gtttctgaga cggccaaatc
     2821 atcaattaaa acgatagcaa atcaaataga ttcaggactt gtaggacaat ttggggaaaa
     2881 agtaaaggca caggtagatt taaataaagg tagttatgat gatttataat cgagtgaaaa
     2941 tgttgaggaa gcttactatc ctgatagtta ttatagatat gaaaataatc aagtt
//
LOCUS       DATRNK010000114         2927 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.115, whole genome shotgun sequence.
ACCESSION   DATRNK010000114 DATRNK010000000
VERSION     DATRNK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.115"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            15..85
                     /locus_tag="VBC52_001992"
     tRNA            15..85
                     /locus_tag="VBC52_001992"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47..49,aa:Gly,seq:tcc)
     gene            120..193
                     /locus_tag="VBC52_001993"
     tRNA            120..193
                     /locus_tag="VBC52_001993"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:154..156,aa:Ile,seq:gat)
     gene            207..294
                     /locus_tag="VBC52_001994"
     tRNA            207..294
                     /locus_tag="VBC52_001994"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:241..243,aa:Ser,seq:gct)
     gene            530..1873
                     /locus_tag="VBC52_001995"
     CDS             530..1873
                     /locus_tag="VBC52_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6883543.1"
                     /translation="MKKRILSAVLVSGVTLGTAAVTVNADDFDSKIAATDSVINTLSG
                     QQAAAQNQVTAIKGQVGALESQQSELEAQNAQLEAVSQQLGQEIQTLSNKIVARNESL
                     KKQVRSAQKGNLTNYINTILNSKSVSDAVNRVVAIREVVSANEKMLAQQEADKAALEA
                     KQIENQNAINTVAANKQAIENNKAALATQRAQLEAAQLELSAQLTTVQNEKASLIQAK
                     AQAEEAARKAAEAQAAAEAKAQAEAKAQAESVAQAQAAAQVESATAPTETVQTQPRTE
                     IKPSNLTATSSATTVAKTTATATNEPKVAQPSVVTKAVEAPKAVVSSTPRSVSKPVVR
                     SYDSSNTYPMGQCTWGAKSMASWVGNYWGNANQWGASARAAGYSVGTTPRVGAVAVWP
                     YDGGGYGHVAVVTSVANNSSIQVMESNYAGNMSIGNYRGSFNPSASGSVYYIYPN"
     gene            1997..>2927
                     /locus_tag="VBC52_001996"
     CDS             1997..>2927
                     /locus_tag="VBC52_001996"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO6883544.1"
                     /translation="MSYSNLKLFALSSNKELAKKVSQTIGIPLGQSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAESVSVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRQ
                     GLVGDDVVVVSPDHGGVTRARKLAQCLKTPIAIIDKRRSVTKMNTSEVMNIIGNIKGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQNSAIEKLIVL
                     DTIYLPEERLIDKIEQISIAELIGEAIIRIHEKR"
BASE COUNT          901 a          505 c          629 g          892 t
ORIGIN      
        1 atataagatt tttagcgggt gtagtttagt ggtaaaacta cagccttcca agctgttgtc
       61 gcgagttcga ttctcgtcac ccgcttactt tacattaaag taaaaccaag catttgcttg
      121 ggcgcgtagc tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt
      181 ccactcgtgc ccatcaatat taatttggag aattactcaa gaggctgaag aggacggttt
      241 gctaaatcgt taggtcgggt aactggcgca agggttcgaa tcccttattc tccgtaaata
      301 ttataatttt aagagtaatt atactctttt tttatttctc ttttagtttc actatggagc
      361 aattatattg atagagtgaa tagaagaagg ttatcgtttt ctagatttga ttatggttca
      421 tttagaaata gttgatttca aatataaatg taacaatgac gtaatataat ttcgtgaatt
      481 ttttggtaaa atatttaatg tcttatcgta aaggagtagt ttaataaaaa tgaaaaaaag
      541 aatattatca gcagtccttg tgagtggagt aactcttgga actgctgccg taacagtaaa
      601 cgctgatgac tttgactcga aaattgctgc tactgattca gtaatcaata ctttatcagg
      661 gcaacaagca gcagcacaaa accaggtgac tgctattaaa ggacaagttg gtgctttaga
      721 gtctcaacaa tcagaattag aagcgcaaaa tgctcaactt gaggctgtat ctcagcagtt
      781 aggtcaagag attcaaactc tttctaataa aattgttgca cgtaacgaat ctttgaaaaa
      841 gcaagttcgt agtgctcaaa agggtaactt gactaactat attaatacta ttttaaattc
      901 gaaatcagta tctgatgctg ttaatcgtgt tgttgctatt cgtgaggttg tttcagctaa
      961 tgaaaagatg ttagcacaac aagaggctga caaagcggcc ttagaagcta aacaaattga
     1021 aaatcaaaat gctataaata ctgtagcagc taacaagcaa gctatcgaaa ataataaagc
     1081 agccttagca acacaaagag cacaattaga agcagctcaa ttagagttat ctgctcaatt
     1141 gactacagtc caaaatgaaa aagcatcttt aattcaggca aaagcacaag ctgaggaagc
     1201 tgcgcgtaag gcagctgaag cacaagcagc agctgaagcg aaagcacaag ctgaagcgaa
     1261 agcacaagct gagtctgtag cacaagcaca ggcagcagca caagttgagt ctgccacagc
     1321 tcctactgaa acagttcaaa cacaaccgag aactgaaata aagccttcta atcttacggc
     1381 tacttcttcg gcaactactg ttgctaaaac tacagctaca gctactaatg agccaaaagt
     1441 tgctcaacct tctgtagtaa ctaaagcagt tgaagcacct aaagcagttg tttcttcaac
     1501 acctagatcg gtatcaaaac ctgttgtcag aagttatgat tcaagtaaca catacccaat
     1561 gggacaatgt acttggggag ctaagtcaat ggcttcttgg gttggtaact attggggaaa
     1621 tgctaatcag tggggtgcta gtgcacgtgc agctggttat tctgtaggaa ctacccctcg
     1681 tgttggtgca gtagctgttt ggccatatga tggtggtggt tacgggcacg ttgcagtagt
     1741 aacgtcagtg gctaacaatt catctatcca agttatggaa tcaaactatg ctggaaatat
     1801 gagcattggc aattaccgtg gttcatttaa ccctagtgct tctggctctg tttactatat
     1861 ttacccaaat taatgaatac taaggagatc tttcgtggtc tcctttttgt ttattaatta
     1921 ggtatggttt attttggatt gaaaaattgt gtaaaaagcg ttaaaatggt ataggactaa
     1981 agttttggag gaaatcatgt cttattctaa tttaaaattg tttgcactgt catcaaacaa
     2041 agaactagcg aaaaaagtgt ctcagacgat tggaattccg ctaggtcaat cgacagttcg
     2101 ccaattttct gatggtgaga ttcaagttaa tattgaagag tctattcgtg gccatcatgt
     2161 ttttatttta caatcaacaa gctcaccagt taatgataat ttgatggaaa ttttaattat
     2221 ggttgacgct ttaaagcgtg ctagtgcgga gtctgttagt gtggtaatgc cttactatgg
     2281 gtatgctcgt caagatcgca aggcacgttc tcgtgaacct attacttcga aactcgttgc
     2341 gaacatgcta gaagttgctg gtgttgatcg tcttttaaca gttgatttac atgcagctca
     2401 aattcaggga ttctttgata ttcccgttga tcaccttatg ggggctcccc ttattgcaga
     2461 ttactttgat cgtcagggat tggttggtga tgatgttgtt gtcgttagtc ctgatcatgg
     2521 tggtgtaaca cgtgcacgta agctggcaca gtgtttaaag acaccgattg ctatcattga
     2581 taagcgccgt agtgttacta aaatgaatac atctgaagta atgaatatta ttggtaatat
     2641 caaaggtaaa aaatgtattt taattgatga tatgattgat actgcaggta ctatttgcca
     2701 tgctgcagac gctttagctg aagcaggggc aacagctgtt tatgcttcat gtactcaccc
     2761 tgttttatca gggccggctt tggataatat tcaaaattca gctattgaaa aattaattgt
     2821 gttagatacg atttacctcc ctgaagaacg tttgattgat aagattgaac aaatttcaat
     2881 tgcggaattg attggtgaag ctattatccg tattcacgag aaacgtc
//
LOCUS       DATRNK010000115         2898 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.116, whole genome shotgun sequence.
ACCESSION   DATRNK010000115 DATRNK010000000
VERSION     DATRNK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.116"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(90..518)
                     /locus_tag="VBC52_001997"
     CDS             complement(90..518)
                     /locus_tag="VBC52_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6883545.1"
                     /translation="MWNVKTFDNLTTHELFQIYKLRVSVFVVEQDCPYQEVDDEDLIC
                     LHGMNWVDGQLAAYYRLIPEDDKVHLGRVIVNPDFRKKGLGNQLVEYAIKFSEANYPN
                     KPIYAQAQAYLQDFYQSFGFQPVSDIYLEDNIPHLDMVLL"
     gene            complement(552..1122)
                     /locus_tag="VBC52_001998"
                     /pseudo
     CDS             complement(552..1122)
                     /locus_tag="VBC52_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771410.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
     gene            complement(1391..2746)
                     /gene="rlmD"
                     /locus_tag="VBC52_001999"
     CDS             complement(1391..2746)
                     /gene="rlmD"
                     /locus_tag="VBC52_001999"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO6883546.1"
                     /translation="MNVVLKQRIPLKIKRMGINGEGIGFYKKTLIFVPGALKGEEVFC
                     QISSVRRNFAEAKLLKINKKSKNRVEPPCSIYKECGGCQIMHLQYDKQLEFKTDVIRQ
                     ALMKFKPEGYENYEIRKTIGMSEPEHYRAKLQFQVRSFGGNVKAGLYAQGTHRLIDIK
                     DCLVQDSLTQEMINRVAELLGKYKLPIYNERKIAGVRTVMIRRAQASGEVQLIFITSK
                     RLDFDDVVIELVREFPELKTVAVNINASKTSDIYGQITEVIWGQESINEEVLDYGFSL
                     SPRAFYQLNPKQTQILYSEAVKALDVKEDDDLIDAYCGVGTIGLAFAGKVKSVRGMDI
                     IPEAIQDAKENALYMGFTNTHYEAGKAEDIIPRWYSEGFRANALIVDPPRTGLDDKLL
                     NTILKMPPEKMVYVSCNTSTLARDLVTLTKVYHVHYIQSVDMFPHTARTEAVVKLQRK
                     E"
BASE COUNT          957 a          574 c          372 g          995 t
ORIGIN      
        1 ttaatttaaa ttttttaatt ttttgttaat taaaagatct ctcagcaata ctgagagacc
       61 ttttaattga tagtttaaat agcatgtatt tataataaaa ccatatcaag atgaggaata
      121 ttatcttcta gatagatatc tgagacaggt tgaaatccga aagattgata aaaatcttgt
      181 aaataagctt gggcctgagc ataaataggt ttattagggt aatttgcctc actaaattta
      241 atcgcatatt ccactaattg atttcctaat cccttttttc taaagtctgg attaacaatc
      301 acacgtccca aatgcacctt atcatcttct ggtatcaatc tataataagc tgccaattga
      361 ccatctaccc aattcatccc atgcaaacaa atcaaatctt catcatcaac ctcttgataa
      421 ggacaatctt gttcaaccac aaatacagat actcttaatt tatatatttg aaacaactca
      481 tgtgtagtta aattatcaaa agtttttaca ttccacattg ctatcttcct ttcaacatca
      541 tacagtagac cctaaaaagt ctctatagca aactccatgg caatataagc aattttatct
      601 ttcttttcta aatcctcgct atttcctaaa gatcttgctt cccatacttc tgcatctaaa
      661 ttatccgcag agtaaaaaaa ttgaatttgt gtttactgca atttcagaac ttgcttccga
      721 ataatgaaat gatgtccctt catccctaat agcacttctt ggaataataa tggatgtctc
      781 ctcaattgac gaatcaagga cgccacatgt tccaaaaaca attaaacttt ccattccaaa
      841 tactataata tcttccaaga ttgctacgca agctgaagca ccaacataag cattgaaaaa
      901 gccaatcttt tgtcctttaa attctaattc ataaatggga atttctaagt ttgctacact
      961 agttctagca atttcacgat gaggcaattc ttctaaaata cgattgaaag tctcccgcgc
     1021 gaaacaggta ataacagtct ttggaaaacc ttttattggt gaattaatat cttcaggatt
     1081 aataatcgct tttctactat tatcgaatga atttattatc ataagcattt cctttaaacc
     1141 ttaatttacg atgttctatc atcttgttaa aaatattttt tctattttaa ctcaatgaaa
     1201 cttaaaaggc aaggtcaaat ttgcaaagat gaatatactt aatggaacta tttaccattt
     1261 ctaaggtaaa tttccgtatc aaatagctta aaccatgtgt ttttagacta tgttatatta
     1321 atatctcacc attactatct aaaaataaga atacccaaaa atactcttat tttttgatta
     1381 gaaaacttta ttattcctta cgttgtagtt tcactacagc ctcggtcctt gctgtatgtg
     1441 gaaacatgtc aacggattgg atgtagtgga catggtatac ttttgttaat gttactaagt
     1501 ctctagcaag cgtggatgta ttacaagaaa cgtaaaccat tttttctgga ggcattttta
     1561 gtatagtatt tagcaattta tcatctaaac ctgtgcgagg aggatccacg attaacgcat
     1621 tagcgcgaaa tccctcacta taccacctag ggataatatc ctctgccttt ccagcttcgt
     1681 aatgtgtatt agtaaaaccc atataaagtg cgttctcttt agcatcctga atagcctcag
     1741 ggataatatc cattccacga acacttttaa cttttccagc aaaagcaagt ccaatcgttc
     1801 ctactccaca ataagcgtca attaaatcat catcttcctt cacatctaag gccttaactg
     1861 cctcactata taaaatttgt gtttgtttag gattaagttg ataaaaagca cgtggtgata
     1921 acgaaaaacc ataatctaat acttcttcat tgatggattc ttgaccccaa ataacttctg
     1981 ttatttgacc atagatatca cttgttttac tagcgttgat atttacagca actgtcttta
     2041 actcaggaaa ctctcgaact aattcaataa caacatcatc aaaatcaagt cttttacttg
     2101 tgataaagat cagttgaacc tctccactcg cttgtgctcg tcgaatcata acagtacgga
     2161 caccagctat tttacgttca ttataaatag gcaatttata ttttcctaaa agttctgcaa
     2221 ctcgatttat catttcctga gtaaggctat cttgcactaa acaatctttg atatcaatca
     2281 atcgatgtgt cccttgtgca tataaacctg cctttacatt ccctccaaac gaacgcactt
     2341 gaaattgtaa tttagcacga tagtgttcag gttccgacat acctatcgtt ttacggattt
     2401 cataattctc atatccctct ggtttaaatt tcattagagc ctgtcgaata acatccgttt
     2461 taaactctag ctgcttatca tattgaaggt gcattatttg gcatccccca cattctttat
     2521 aaatagaaca agggggttct accctatttt tagatttttt atttatcttt aacagtttag
     2581 cttctgcaaa attacgtcta acagaagaaa tctgacaaaa cacctcttct cccttgaggg
     2641 cccctggaac aaagattagt gtttttttat aaaaacctat tccttcacca ttaattccca
     2701 ttcgtttaat ttttaaagga attcgctgtt ttaaaaccac attcatcagt tcattactcc
     2761 ctaactaaga aaatactata aatccataac atttaaaaaa aagactcatt tttactattt
     2821 tatcattttt gctataataa aaactatgaa aataaccaaa atcgaaaaga aaaaaagact
     2881 ttacactcta gagctaga
//
LOCUS       DATRNK010000116         2816 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.117, whole genome shotgun sequence.
ACCESSION   DATRNK010000116 DATRNK010000000
VERSION     DATRNK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.117"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..575)
                     /locus_tag="VBC52_002000"
     CDS             complement(<1..575)
                     /locus_tag="VBC52_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6883547.1"
                     /translation="MTDKLVSVIIPVYNAAPYLEGCVNTILGQTYQVFEILLIDDGST
                     DTSASICDQLSLRDNRIRVFHIENGGASKARNFGLARISSESQFVTFVDSDDWVKENY
                     LEVLLAQQEKYNADIVISNYYIYRETEDIFGYYITDKDFVIEEISAQTAIDRQVHWHL
                     NSSVFIVIWGKLYRRELFDTITFPIDKVFED"
     gene            complement(568..1752)
                     /locus_tag="VBC52_002001"
     CDS             complement(568..1752)
                     /locus_tag="VBC52_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEO6883548.1"
                     /translation="MKNNVICLAGDNKSLNQIQTVIKSILCHNDRVSIYILNQDIASE
                     WFRNIQRRLLNSHSCIFDIKLFDDTFKEFKTPRAHITYMAYARYYIPQLIDAEKVLYL
                     DIDTLVVDNLDKLFEIELGDYPIAAILDGDGIHFNSGVMLINSLYWMRYRVTEKLMEI
                     TERELDNGIFGDQGVLNLLFDNNWLKLEDKYNAQVGNDLGAFYENWQGYFDRNFESPT
                     IIHYCTHDKPWNTFSSSRFRETWWQYEQLDWNEVFNFETYLLPEPTFEKHFFTFTTSV
                     DLLYIEELVELFPNSCFHIAAWTSFGPSLLKLATNSNVRLYPSITSPLFEQLMTKANY
                     YLDISTSWKEIQFCQKAIEKSIPILSFNEAVTFEYRELSHCFINLEDMRQFLCKNGGN
                     SD"
     gene            complement(1742..>2816)
                     /locus_tag="VBC52_002002"
     CDS             complement(1742..>2816)
                     /locus_tag="VBC52_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6883549.1"
                     /translation="MDCTISNTKIFNEEIKHFKFQKPMPYPTYFRYFIPEVIHEDKVL
                     YLDCDMIITSDLTSIFTLDISKYGVAAVRDDLLEEYDGKEDYFNSGLLLINNIFWREQ
                     GFSQRLLDYTRENQGALQYHDQDVLNDVLCDNWLELDETYNYHTGADMLYNLFQQSER
                     QLNRRKDLPKVIHYTATKPWKYLETSVRWRDIWWEYNRLEWRDIFTRWQVNDGVDVSL
                     KKVLQPIHRAFIFTYSDSIEGIERLAKDLPNWQFHIAAYTQISEKLKQLEMHSNIKLY
                     PLVYRFKLNELVENSDIYLDINHGDADTQVLEQVKKLGKDILSLSTTYHGQGKQFERV
                     TDIIDYLKNERFKEHIRKEYCEK"
BASE COUNT          945 a          517 c          403 g          951 t
ORIGIN      
        1 tcatcttcaa atactttatc tattgggaag gtgatggtat caaatagttc tcttcgatat
       61 aatttccccc aaataacgat gaatactgaa ctatttaaat gccaatgcac ttgtctatca
      121 atagctgttt gagcagagat ttcttcgata acaaaatctt tgtcagtgat gtagtaacca
      181 aaaatatctt ctgtttctct gtagatatag taattactga taacaatatc agcattatat
      241 ttttcttgct gagctaacag tacttccaaa taattttcct ttacccaatc gtccgaatca
      301 acaaacgtaa caaattggct ctctgaacta attcgtgcta atccaaagtt tctagcttta
      361 gaagcaccgc cattttcgat atggaaaact ctaatacggt tatctcttag agataattga
      421 tcgcagatac tagcagatgt atctgtagaa ccgtcatcta tcagtagaat ttcaaaaact
      481 tggtatgttt gtcctaaaat ggtatttaca cacccctcta aatatggtgc tgcattatag
      541 actggaataa taacagaaac taatttatca gtcactattg cctccatttt tacataaaaa
      601 ctgtcgcata tcctccaaat taataaagca atgacttaac tccctatatt caaatgtcac
      661 tgcttcatta aaactcaata tcggaattga tttctcaata gctttttgac aaaattgtat
      721 ttccttccaa gaagttgaga tatcaaggta ataatttgct ttagtcatta gttgttcaaa
      781 aagtgggctg gttattgaag gatacagacg aacgttagag tttgtggcta acttaagtaa
      841 gcttgggcca aaagaggtcc aagcagctat atggaagcaa ctattgggga atagttcaac
      901 caattcttca atataaagca aatccacgga cgttgtgaaa gtaaagaagt gtttttcaaa
      961 tgttggctca ggtaacagat acgtttcaaa attaaacacc tcattccaat ctagttgctc
     1021 atattgccac catgtctccc taaaccttga agaagaaaaa gtattccatg gtttatcatg
     1081 agtacagtaa tggataatag taggcgattc aaagttacga tcaaaatacc cttgccaatt
     1141 ttcataaaag gcacctaaat catttccaac ttgagcatta tacttatctt ctaacttaag
     1201 ccaattatta tcaaaaagaa gattgagaac cccttggtct ccaaaaattc cattatccaa
     1261 ttctcgttct gttatttcca taagtttttc tgttactcga tatctcatcc agtaaagcga
     1321 gtttattagc ataacacctg agttaaaatg aataccgtct ccatctaaga tagctgctat
     1381 cgggtagtcc cctaattcta tctcaaacaa cttatctaag ttatcaacaa ccaaagtatc
     1441 aatatctaaa taaagaactt tctctgcatc aatcagttgc ggaatgtagt accttgcata
     1501 agccatatag gtaatatggg cacggggcgt tttaaattcc ttaaaagtat catcaaagag
     1561 ttttatatca aaaatacaac tatgactatt taataaacgt ctttgaatat ttctaaacca
     1621 ttctgaagct atatcttgat ttaaaatata aatacttact ctatcattgt gacataaaat
     1681 cgattttata actgtttgga tctgatttaa agatttatta tcacctgcca aacaaataac
     1741 gttatttttc acaatattcc tttctaatat gttctttaaa tcgttcattc tttaaataat
     1801 caataatatc cgtaactctc tcaaattgtt tgccttgacc atgataagtt gtagaaaggg
     1861 ataaaatatc tttacctaat ttcttcactt gctccagaac ctgagtatct gcatcaccat
     1921 gattaatatc aaggtaaata tctgaatttt caactagctc atttaatttg aaacgataaa
     1981 caagtggata aagttttata tttgaatgca tttccaattg tttcaatttt tctgaaattt
     2041 gagtataggc agcaatatga aattgccagt ttggtaaatc cttagccaac ctctcaattc
     2101 cttcaattga atctgagtaa gtaaaaatga aagctcgatg gataggttgt aacactttct
     2161 tcaatgaaac atctacacca tcattgactt gccatcttgt aaaaatatcg cgccattcaa
     2221 gtctattata ttcccaccat atatcgcgcc aacgaacaga tgtttcaagg tatttccatg
     2281 gttttgtcgc tgtataatga ataactttag gtaaatcttt cctgcgattt aattgacgtt
     2341 cagactgttg gaataggtta tataacatat ctgctcctgt atgatagtta taagtttcat
     2401 caagttctag ccaattatcg cacagtacat cattcaaaac atcctggtca tgatattgaa
     2461 gagccccttg gttttctcta gtataatcta acagtcgctg actaaaccct tgttctcgcc
     2521 aaaaaatatt attaattaat agcaaacctg aattaaaata atcttcttta ccatcatatt
     2581 cttcgagcaa atcatctcga acagccgcta caccatattt tgaaatatca agagtaaata
     2641 tcgatgttaa atcactggtg ataatcatat cacagtctaa atatagtacc ttatcttcat
     2701 gaataacttc aggtataaag tatcgaaagt aagtaggata gggcatcggt ttttgaaatt
     2761 taaaatgttt aatttcttca ttaaaaattt tagtgttaga tattgtgcaa tccatt
//
LOCUS       DATRNK010000117         2785 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.118, whole genome shotgun sequence.
ACCESSION   DATRNK010000117 DATRNK010000000
VERSION     DATRNK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.118"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            138..437
                     /locus_tag="VBC52_002003"
                     /pseudo
     CDS             138..437
                     /locus_tag="VBC52_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
     gene            1364..1924
                     /gene="efp"
                     /locus_tag="VBC52_002004"
     CDS             1364..1924
                     /gene="efp"
                     /locus_tag="VBC52_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEO6883550.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNNETYDQYEIPTVNIENE
                     LLYILENSEVKIQFYGTEVIGVQIPITVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            2013..2402
                     /locus_tag="VBC52_002005"
     CDS             2013..2402
                     /locus_tag="VBC52_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6883551.1"
                     /translation="MTTENLGEIVISPRVLEVITGIAATKVDGVHSLRNKAVTDSLSK
                     KSLGRGVYLKNEEDDTVAADIYVYLQYGVNVPAVSIAIQQAVKTAVYDMAEVKISSVN
                     IHVEGIVPEKTPKPDLKSLFDEDFLDD"
     gene            2395..>2785
                     /gene="nusB"
                     /locus_tag="VBC52_002006"
     CDS             2395..>2785
                     /gene="nusB"
                     /locus_tag="VBC52_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEO6883552.1"
                     /translation="MTSVFKDSRRDLRERAFQTLFSLETGGEFIDAAHFAYGYDKTVS
                     EDKVLEVPIFLLNLVNGVVDHKDELDTLISSHLKSGWSLERLTLVDKSLLRLGLYEIK
                     YFDETPDRVALNEIIEIAKKYSDETSAK"
BASE COUNT          995 a          427 c          503 g          860 t
ORIGIN      
        1 ggcattgtgg gacgaaaact tttcaaactt atttttattt ggatttattt tttcaaacag
       61 tttatttttt ataaagttca ttgagtttca accatcgtta aatggaaaaa aattattata
      121 tcggggagaa ctgaagtatg ttattaccag tagggagtgt tgtctatctt attgatggca
      181 atcaaaaatg agttattgta aatagaggtg caattgttga gcaagaagga caagaagttt
      241 attttgatta cctaggtgga atttttcctg agggtttaaa ccttgaacaa gtttactatt
      301 ttaatcaaga agatattgat gaagttgttt ttgaaggtta ccatgatgag gaggaagaaa
      361 gagtttctcg tctcattgaa aaatggaaaa ataaagaggg aaagaatctt ccaaagggaa
      421 agactagtgt agaatagaaa taatctaaaa agtaaattct actcaaaaac aaatcaatat
      481 gctactagtg tgaagatgct attgatagat gtcatgatac acttatcaga agaaaacgaa
      541 aaaggcttta ctaaattaca attctttact cgacaaattg tggttggtgt aaactggtat
      601 gtacttccca aaattgataa ctatttcaac ccctaaaaga taagaaaaaa tacttttgaa
      661 aatagactgt actttaggaa aatcccgtat aaaagaatag ctaataaagc cagcgcaggt
      721 atctgtacga ttagaaaacg acatagttgt aactttctat aatgagacat attaaaattc
      781 atcattccac ctcctaaatt agtaattcaa tgatcagtgg gctagagctc cttcaatacc
      841 aatgacagct cttattccaa ttgcatatgg cactaaaaca gagagctacc tgatgtagta
      901 agcaagattg gtatcaccaa aatcagtacc aatgcttagc gcacgtccac caacaccccc
      961 ctgatttcct ttacagcttc aatagagcaa attaagttcg tatctaaaac ttatatcgag
     1021 aatagctaca ttttcatacg aatagctccg taggtgattt attttatctg tttcaataag
     1081 acaactatag tttactacct ttatcaaaat ttactaaaaa gtcaccaaaa acacctaaac
     1141 agtctttcaa ttttagtcaa gatatcggta taatattaaa actgttatca aaactgaagt
     1201 aagaggaaag tgtcaagtgc ttcagtctta tttacgccct cttggtgatt ttatttattt
     1261 atcaagaaat cagacacata ccaaatcaat aaactatttg agaaagcagg ttatttgtga
     1321 taaaatgagt tgataaataa tttttacaaa gaggtattaa taaatgattg aagcaagtaa
     1381 gcttaaagca ggcatgactt ttgaaacggc tgatgggaaa ttaatccgtg ttcttgaagc
     1441 tagccaccac aaaccaggta aaggtaacac aatcatgcgt atgaaattgc gtgatgttcg
     1501 tactggttca acttttgata caagttaccg tcctgaagaa aaatttgagc aagctattat
     1561 tgaaactgta cctgctcaat acctttataa gatggatgat acagcttact tcatgaataa
     1621 tgaaacttac gatcaatatg aaattccaac tgttaacatt gaaaatgaat tactctacat
     1681 tcttgaaaac tcagaagtaa aaattcaatt ctatggaaca gaagttattg gtgttcaaat
     1741 tccaataact gttgaattga cagttgctga aactcaacca tcaatcaaag gtgctactgt
     1801 aactggttca ggaaaacctg caactatgga aacaggactt gttgttaacg ttccagactt
     1861 tattgaagct ggacaaaagt tagtaatcaa tacagcagaa ggaacttacg tttcacgcgc
     1921 ctaatgactc tcaaaaaagt gttaagtaga ttcatgatga atttactcta ctaaggaaaa
     1981 accttagtaa acactaaaag aaaggataag ctatgacaac tgaaaatctt ggagaaattg
     2041 tcatttcacc acgtgtactt gaggtaatta caggtattgc tgcaactaaa gttgatggtg
     2101 ttcattcgtt acgtaacaaa gcggtaacgg atagccttag taagaaatct ctaggtagag
     2161 gtgtctacct taaaaatgaa gaagatgata cagtagctgc agatatttat gtttacttgc
     2221 agtatggtgt aaatgtacca gcagtttcaa ttgctattca acaagctgtc aaaacagctg
     2281 tatatgacat ggcagaagta aaaatttctt cggtcaatat ccatgttgaa ggtattgttc
     2341 cagagaaaac accaaaacca gacttgaagt cattgtttga tgaggatttc ttagatgact
     2401 agtgtattta aagattcaag acgtgattta cgtgaacgtg cttttcaaac actatttagc
     2461 ttagaaactg gtggagagtt tattgatgcg gctcactttg cttatggtta tgataaaaca
     2521 gtatctgaag acaaagtttt agaggttcca atctttttac ttaatcttgt taatggtgtt
     2581 gtagatcaca aggatgaatt agatactttg atttctagtc acttaaaatc aggatggagc
     2641 ttagaacgtc taacattggt tgataaatct ctcttacgct taggcctcta tgaaattaaa
     2701 tattttgatg aaacgcctga tcgtgtagct ctaaatgaga ttatcgagat tgctaaaaaa
     2761 tattcagatg agacgtcagc aaaat
//
LOCUS       DATRNK010000118         2703 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.119, whole genome shotgun sequence.
ACCESSION   DATRNK010000118 DATRNK010000000
VERSION     DATRNK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.119"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..590)
                     /locus_tag="VBC52_002007"
     CDS             complement(<1..590)
                     /locus_tag="VBC52_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6883553.1"
                     /translation="MANMYYTENPNVEHDIHELNVKLLGESFSFLTDAGVFSKRMIDY
                     GSQVLLNSLHFEKNKSLLDLGCGYGPLGISLAKVQGVKATMVDINTRALELAKKNATR
                     NGVVVEVFQSNIYENISKTFDYIISNPPIRAGKQVVHSIIEESICYLNTGGSLTIVIQ
                     KKQGAPSAKAKMLDTFGNCDILKKDKGYYILRSEKV"
     gene            696..1616
                     /locus_tag="VBC52_002008"
     CDS             696..1616
                     /locus_tag="VBC52_002008"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEO6883554.1"
                     /translation="MNNEFINFDRISRENWKDLHQQSQALLTEKELESIKSLNDNINI
                     QDVIDIYLPLINLIQIYKRSQENLSFSKAIFLKKENYQRPFIIGISGSVAVGKSTTSR
                     LLQLLISRTFKDSHVELVTTDGFLYPNEKLIQNGILNRKGFPESYDMESLLNFLDTIK
                     NGITAKIPIYSHEIYDIVPNQLQTIETPDFLILEGINVFQNQQNHRLYMNDYFDFSIY
                     IDAENKQIEEWYLQRFNSLLQLAEADPSNYYHKFTQIPPHKAMELAKDIWKTINLVNL
                     EKYIEPTRNRADFIIHKGKHHKIDEIYLKK"
     gene            1670..1918
                     /locus_tag="VBC52_002009"
     CDS             1670..1918
                     /locus_tag="VBC52_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEO6883555.1"
                     /translation="MEVKTLANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANP
                     SEELYRAASSSIDKAASKGLIHTNKASRDKARLATKLG"
     gene            complement(2002..>2703)
                     /locus_tag="VBC52_002010"
     CDS             complement(2002..>2703)
                     /locus_tag="VBC52_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6883556.1"
                     /translation="ITIGFDNTFVPMGFESRSGDYIGFDIDLANAVFKEYGISVKWQP
                     INWDMKETELNNGNIDLIWNGYSKTAERAKKVAFTNPYMNNHQVIVTKTSSHINSIKD
                     MKGKKLGAQSGSSGFDAFNAKPDILKKFVKGKEAVQYDTFTQALIDLKNNRIDGLLID
                     EVYANYYLKQEGNIKAYYFVKTAYQGENFVVGARKVDRRLIEKINKAFKQLHNKGRFQ
                     KISYKWFGEDVYSKE"
BASE COUNT          954 a          488 c          356 g          905 t
ORIGIN      
        1 tagacttttt cacttctcaa tatataatat cccttatctt tttttaaaat atcgcaatta
       61 ccaaaagtat ctagcatttt agcttttgca cttggagcgc cttgtttctt ttgaatgaca
      121 atggtaaggc taccgcctgt attaaggtaa cagatacttt cttcgataat agaatgaaca
      181 acttgctttc cagcacgaat aggtgggttg cttataatat agtcgaaagt tttagaaata
      241 ttttcataaa tgtttgactg aaagacttca actactactc cattgcgtgt tgcatttttc
      301 ttagctaatt ccaatgctct cgtattaatg tcaaccatag ttgctttaac tccttgcact
      361 ttggctagag aaattcccag cggaccgtat ccacacccaa ggtctaacaa ggatttattt
      421 ttttcaaaat gtaaactatt tagaaggact tgactaccat aatctatcat tcttttagag
      481 aaaactcctg catcagttaa aaaactgaat gattctccta ataatttgac atttagctca
      541 tgaatatcat gttcaacatt aggattctct gtataataca tattagccat aaataattct
      601 aaccctccaa tttcgccata ttattttacc atattttgaa acggttttca atcactcaat
      661 aaaatgttat aatagctttc atataggagt ttgacatgaa taatgaattt attaatttcg
      721 accgtatttc cagagaaaat tggaaggact tacatcagca atcccaagct ttactaactg
      781 aaaaagagct tgaatctatc aaaagcttaa acgataatat taatattcaa gatgttattg
      841 acatctacct tcctttaatt aaccttattc aaatctataa acgtagtcaa gaaaatttat
      901 ctttttctaa agcaatattt cttaaaaagg aaaattatca acgtcctttt attatcggca
      961 tatcagggtc agttgctgtt ggaaaatcaa ctaccagtcg cttactacaa ttactaattt
     1021 cacgaacttt taaagatagt cacgtcgaat tagtcactac tgatggcttt ttatatccta
     1081 acgagaaact catacaaaat ggaattttaa accgtaaagg tttcccggaa tcctatgata
     1141 tggagtcact tctaaatttc ttagatacta taaagaacgg tataaccgcg aaaatcccaa
     1201 tttattctca tgaaatctac gatatcgttc caaatcaatt gcaaactatt gaaacaccag
     1261 attttttaat tttagaagga ataaatgtct ttcaaaacca acaaaaccat cgactttaca
     1321 tgaatgatta ttttgatttt tcaatataca ttgatgctga aaataaacaa attgaagaat
     1381 ggtatttaca acgatttaac agtcttctac aattagccga agcagaccct agtaattact
     1441 atcataaatt cacacaaata ccgccacata aagctatgga actagctaaa gatatttgga
     1501 aaactattaa tttagtcaac cttgaaaaat atatagaacc aaccagaaat agggctgatt
     1561 ttattattca taaagggaaa catcataaaa ttgatgaaat ttatctcaaa aagtgataaa
     1621 gggttgccaa aaatcactta attagatata cttatatagt tagattaata tggaggtgaa
     1681 aacattggca aatattaaat cagctatcaa acgcgctgaa cttaacgtta aacaaaacga
     1741 aaaaaactca gcacaaaaat cagctatgcg tactgctatt aaagcatttg aagctaaccc
     1801 ttcagaagaa ctttaccgcg ctgcttcttc aagcatcgac aaagctgctt ctaaaggttt
     1861 aattcacaca aacaaagcaa gccgcgataa agcacgtctt gcaactaaac ttggttaatt
     1921 aacctagaaa acatcggata tatcgatgtt ttttatttaa ttttttcgta gaataaaaga
     1981 ctaggagaaa ttcctagtct tttattcttt actataaaca tcttcaccaa accatttgta
     2041 agagattttt tgaaatctcc ccttattatg aagctgtttg aaagctttgt taatcttttc
     2101 aattagtcta cgatcaactt tacgagctcc tactacaaaa ttttctcctt gataagcagt
     2161 tttaacaaaa taataagctt ttatatttcc ttcttgcttt aaataatagt tagcataaac
     2221 ttcatcaatc aaaagaccat caatacggtt attttttaaa tcaatcaaag cctgagtgaa
     2281 agtatcgtat tgaactgctt cttttccttt tacaaacttt tttaaaatat caggtttagc
     2341 gttaaaagca tcaaaaccag atgaacccga ctgggctcct agttttttcc ccttcatatc
     2401 cttaatacta ttaatatgtg atgaagtttt agtaacaatt acttgatgat tattcatata
     2461 tgggtttgta aaagcgactt ttttagcacg ttctgccgtt tttgaataac cattccaaat
     2521 aaggtctata ttaccattat taagttcagt ttctttcata tcccagttaa taggctgcca
     2581 tttcactgaa ataccgtatt ctttaaaaac agcattagct aaatcaatat caaagccgat
     2641 atagtcacca gaacgacttt caaatcccat aggaacaaaa gtattatcaa atccaatagt
     2701 aat
//
LOCUS       DATRNK010000119         2639 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.120, whole genome shotgun sequence.
ACCESSION   DATRNK010000119 DATRNK010000000
VERSION     DATRNK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.120"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..108)
                     /locus_tag="VBC52_002011"
     CDS             complement(<1..108)
                     /locus_tag="VBC52_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEO6883557.1"
                     /translation="MRVYENKEELKKEISKTFEKYIMEFNNIPENLKDKR"
     gene            complement(429..1166)
                     /gene="aphA"
                     /locus_tag="VBC52_002012"
     CDS             complement(429..1166)
                     /gene="aphA"
                     /locus_tag="VBC52_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEO6883558.1"
                     /translation="MKKVLVSSLLVLGITITLQTVVEAKGPKVAYTQEGMTALSDTNK
                     DKVTTISIDEIQKSLEGKKPITVSFDIDDTLLFSSQYFQYGKEYVTPGSFDFLHKQKF
                     WDLVAKRGDQDSIPKEYAKKLIAMHQKRGDKIVFITGRTRGSMYKEGEVDKTAKALAK
                     DFKLDKPIAVNYTGDKPKKPYKYDKSYYIKKYGSDIHYGDSDDDIHAAREAGARPIRI
                     LRAPNSTNLPLPEAGGYGEEVLENSAY"
     gene            complement(1284..1505)
                     /locus_tag="VBC52_002013"
     CDS             complement(1284..1505)
                     /locus_tag="VBC52_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883559.1"
                     /translation="MPTMQLLDELVLPKDITIADVSQSIQEFEIERDALQEFFDDMLK
                     TYTTYQEMKDYIKSREANTPKQETEEKSL"
     gene            complement(1746..>2639)
                     /locus_tag="VBC52_002014"
     CDS             complement(1746..>2639)
                     /locus_tag="VBC52_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEO6883560.1"
                     /translation="ILDDFVKESFLTEDSQINIIRQILYSMSLVHQRGVLHRDLSPTN
                     IFFINGIIKLADFGLGKNLNTLTSHQTMDTASFGQLFYCAPEQLTLLKDADKRSDVYS
                     LGRIINFVMTKNPNDFSHSLRSISEKATNLNPEYRYEDATNMLEKLNRLVSIRGDEKY
                     EVMIWDKISNQKIDSDVENYIYELSGIDLCQKCMQKGNIFLDALFEFVKIDDSHAIYI
                     VQQIGSHYKTQLKKFEDADIFANFAYRILKGNFTYTINEVAATILRYVAFEVNRFNAQ
                     HKIDDLKARGLEPLIEDILER"
BASE COUNT          829 a          472 c          363 g          975 t
ORIGIN      
        1 tcttttatct tttagattct ctggaatatt attaaattcc ataatgtatt tctcaaatgt
       61 tttacttatt tcttttttca actcttcttt attttcataa actctcaaaa aacatctcct
      121 ttctgataca tattattaat aatgatacgg aaacaacccc aaagtattgt attatagaat
      181 tagatgactg attacaaatt ccttgttcac gccaaaagag ttattaaaaa attatgattc
      241 aattttttta gacaggcatt tgttagaaaa acttcttgtt accgagaggg gagaagatca
      301 gtcattaata atcaatctaa actactccaa cttcccttat tagcatatca ttgatttgct
      361 acttttttag cacttcttat tacaaatcta aaaattgata gataatacta tgttaactaa
      421 taaagatact agtaagctga attttcgaga acctcttcac cgtagcctcc agcttctggt
      481 aaaggtagat ttgtagaatt aggtgctctt aaaattctaa ttggtctagc accggcctcc
      541 ctagctgcat gaatatcgtc atcactatct ccataatgaa tgtctgaacc atatttctta
      601 atataatatg atttatcata tttgtatggc tttttaggtt tatcgcctgt ataatttaca
      661 gcaattggtt tgtctaattt aaaatcttta gctaaggctt tagctgtttt atcaacctcg
      721 ccctccttat acattgaccc tcttgtccta cctgttataa aaacaatttt atctcctcgt
      781 ttttgatgca tagcaattaa ttttttagca tattctttgg gaatggaatc ttgatctcct
      841 cgttttgcaa caagatccca gaatttttgt ttatgaagaa aatcaaacga tccaggagtt
      901 acatattctt taccatattg aaaatattga ctactgaaaa gcagtgtatc atcaatatca
      961 aaactaacag taatcggctt cttaccttct aagctttttt gaatctcgtc aatagaaata
     1021 gtagtgactt tatctttatt tgtgtccgaa agagcagtca ttccctcttg tgtataagct
     1081 acttttggcc ccttagcctc aactactgtt tgtaacgtta tcgtaatccc taaaaccaaa
     1141 agactactca ctaacacttt cttcataaga acctccataa taaagtataa aaattatttc
     1201 tttaattagt ttatcacaaa aaacataaga atgaaagcga tatcatatat ggttattttt
     1261 tacttatgtt aagtctacag tcttcaaagt gatttttctt ctgtctcttg cttaggagtg
     1321 ttcgcttcac gagatttaat ataatctttc atttcttggt aggttgtata agtcttcagc
     1381 atatcgtcaa aaaattcttg aagggcatct ctttctattt caaattcttg gatactttga
     1441 ctgacatctg ctatggtaat gtcttttgga agaactaact catctaacag ctgcatagtt
     1501 ggcaaactat ttcctggtgt ttgtgcaatg gcaagtagag ccccttcgat tttttggtaa
     1561 taggtcagtc gattatctaa ggctttttaa tattcctttc gttaaaaagt ttctaggctt
     1621 ttacgagcag agccacacac tccacatgtg ttgataggta ttacctatat tatggaacga
     1681 aatgagattg gtcaaaatta ggattatact atggaacgaa aataattctc tttgctagca
     1741 ataattcatc tttctaagat gtcttcaatt aaaggttcta atccacgtgc ctttaaatca
     1801 tcgattttgt gttgcgcatt aaatcgatta acttcaaaag caacatatct caatattgta
     1861 gctgcaactt catttatagt ataagtaaaa ttccctttca aaattctgta ggcaaagttt
     1921 gcaaaaatat ccgcatcttc gaatttcttt aactgagttt tataatgaga gcctatctgt
     1981 tggactatgt agattgcatg actatcatct atttttacaa attcaaatag cgcatctaaa
     2041 aaaatattac ctttctgcat acacttttga cataagtcta taccagataa ttcatagata
     2101 taattttcca catccgaatc aattttttga tttgaaatct tatcccatat cattacttca
     2161 tatttttcat cacctctgat actaactaat ctattgagtt tttctaacat gttagtagca
     2221 tcttcatatc gatattctgg atttaaatta gttgctttct cactaattga acgtaatgag
     2281 tgggaaaaat cgttaggatt ttttgtcatt acaaaattta ttatacgtcc tagtgagtaa
     2341 acatcactgc gtttatcagc atctttcaac agggtaagtt gctctggagc acaataaaaa
     2401 agttgcccaa aagacgccgt atccatagtt tgatgagaag ttaacgtatt cagatttttc
     2461 cccaatccaa aatcagctaa tttaattata ccatttatga agaatatatt ggtcggactc
     2521 aaatctctat gtagcacgcc tctttgatga actagtgaca tcgaatacaa aatttgtcga
     2581 ataatattaa tttgagaatc ctctgtcaaa aaactttctt taacaaagtc atccaatat
//
LOCUS       DATRNK010000120         2604 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.121, whole genome shotgun sequence.
ACCESSION   DATRNK010000120 DATRNK010000000
VERSION     DATRNK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.121"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            136..417
                     /locus_tag="VBC52_002015"
     CDS             136..417
                     /locus_tag="VBC52_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEO6883561.1"
                     /translation="MKLTPEVLRASAQKYTIGSQSITDVLTALTQEQAVIDENWDGTA
                     FDSFEAQFNELSPKITQFAQLLEDINQQLLKVADVVEQIDSDIASQINQ"
     gene            518..901
                     /locus_tag="VBC52_002016"
     CDS             518..901
                     /locus_tag="VBC52_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 family protein"
                     /protein_id="HEO6883562.1"
                     /translation="MKKKYWILLILLLTVLGGSGYMVFKQHTEKEKMIAIAHSDEAKK
                     VYESFMKKRDNSAFTDEGIIKKYTIDDNSVYSNPMGGIIVKVIVNDSSKNYISYNLIK
                     DDEGRMHYANLVVSADLSNKLGEPK"
     gene            1180..1908
                     /locus_tag="VBC52_002017"
     CDS             1180..1908
                     /locus_tag="VBC52_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883563.1"
                     /translation="MNKSKVVLAVCVVGISFLAAMNSDIDSVSANAVMNSNYIPQTSN
                     IIISSEKISPYVIEFVTQQESNNITSYARSLQSQEEENFNTVRFDFTQAEFYDENGNL
                     VDKDTVMETVRPEKLLARSVGTSGGTWFSGSGYNVCKGMRVSGNAGLVGLQSSYYVDF
                     QKVQGGYNRLDRVYGATVDRAGTWTFLANGVFRGSEAPGASAYGGIKGQWSVSPGFGL
                     PTGTSTKYLYFRVGNDTFWLDTNF"
     gene            2202..2396
                     /locus_tag="VBC52_002018"
     CDS             2202..2396
                     /locus_tag="VBC52_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEO6883564.1"
                     /translation="MSTVAVRVDDQLKDDATKLFQSLGLDMSTAVKMFLIQSVKTQSI
                     PFEIKNNPSILVEELQNKEF"
BASE COUNT          947 a          315 c          465 g          877 t
ORIGIN      
        1 ccaaaaggag accgtttttt aagttattat ttaaaaatta aataaattgc ggattaatac
       61 tcttattttt aacgttttat cgtttaaaat attatataaa ttatataaaa gaggatacaa
      121 tatgtcacaa attaaattaa attaactcct gaagtactcc gtgcgtcagc acaaaaatac
      181 acaataggat cacaatcaat tacagatgtg ttaactgctt taacgcaaga gcaagcagtt
      241 attgatgaga attgggatgg tacagcattt gacagttttg aagctcagtt caatgaattg
      301 tcgccgaaaa tcacacaatt tgcacaattg ttagaagata tcaatcaaca actattaaaa
      361 gtagcagatg ttgtagaaca aatagactct gatattgctt cgcagattaa tcagtaactg
      421 gcttattccc attcataagt atagcttagg agggcataag ccttcctatt tttgatatac
      481 ttaaaagtga gtatcaatta tatcgaagga gttaatgatg aagaaaaagt attggatttt
      541 actaatatta ctattaacag ttttgggagg aagtggctat atggttttta aacaacatac
      601 agaaaaagaa aaaatgattg caatagctca tagtgatgag gcaaaaaaag tttatgaatc
      661 atttatgaaa aaaagagata atagtgcgtt cacagatgaa ggaataataa aaaaatacac
      721 aattgatgat aatagcgtat acagtaatcc aatgggaggc attattgtaa aagttatagt
      781 taatgattca tcaaagaatt atataagtta taatttaata aaagatgacg aaggaaggat
      841 gcattatgca aatcttgtcg tatcagctga cctatcaaac aaacttggag aaccaaaatg
      901 atgaaaaagt atgttaagga tgatagtctt caaatagcta tgactgaata taatgaattg
      961 aattcagtta acgatgaagt ttggacagag gatattttat agagtataca aatttatttg
     1021 aatttgtttg ttgtataaaa ataaatggta aaatgacaaa catacgcctt aataattcag
     1081 atgttaaaca aaacagaaaa cgctctcctt tgtgagtgat taagtgtata attgaattaa
     1141 agtaatattt taatttcttt tttataaagg aagatgacta tgaacaagtc aaaagttgtg
     1201 ttggctgttt gtgtagttgg aataagtttt ttagcagcaa tgaattctga tattgactcg
     1261 gtaagtgcta atgcagttat gaattctaat tatattccac aaacttcaaa tataattatt
     1321 tcatctgaaa aaatttctcc atacgttata gagtttgtta ctcaacagga atccaataat
     1381 attacttcat atgcacgttc cttacaatct caggaggaag aaaatttcaa tactgtcagg
     1441 tttgacttta cacaagcaga attttatgat gaaaatggga atctggttga taaagatact
     1501 gttatggaaa cagtaagacc agaaaaattg ttggctaggt ctgtaggcac aagtggtgga
     1561 acttggtttt caggatcagg ttataatgta tgtaaaggaa tgcgtgtttc aggtaatgca
     1621 ggattggtcg gtttacaatc aagctactat gtagattttc aaaaggttca aggagggtat
     1681 aatagactcg atagggtata tggtgccaca gttgatagag caggaacttg gaccttttta
     1741 gctaatggtg tatttagggg tagtgaagca cccggagcct cagcttatgg tggtataaaa
     1801 ggtcagtggt ctgtctcacc agggtttgga cttcctactg gtacatctac aaaatatcta
     1861 tacttcagag ttggtaacga cactttttgg ttagatacta atttttaaag agaaattaat
     1921 taagaggttt ttatggcaga atttatgtta tatttaggaa tattattgaa tttattgtat
     1981 ttaggacttg ctattacagc agtaatttac cttataaaaa tatataaatt actttcagaa
     2041 aaaaatcatt agataaaaag agagatattt tctaaagatt aaaatattac ttttcgtttc
     2101 aaagggagct tgcatgcaat gtaggctctt taataagttg tttgagaatt tgtttgcatc
     2161 gtatttctat tagttataca aagaaaggta agatgatttg tatgtcaaca gttgcagtaa
     2221 gagttgatga tcagttaaaa gatgatgcaa caaagctttt tcaatcttta ggattagata
     2281 tgagtactgc tgtgaaaatg tttctcattc aatctgttaa aactcaaagt ataccttttg
     2341 agattaaaaa taatccttca attctagttg aagaattaca aaataaggag ttttaatgat
     2401 gaaaaagaaa tttttaggat tgttagtttt aactttatat tgtctacact atatacttct
     2461 ctagagagtt tattataaac cttttcagaa accatcattt atcctcataa tagattattg
     2521 aaatatcatc actatttttt tgcttgtttc atttaaaatt cgaaaatact ttggtgaata
     2581 attcgataat tctatttctc cact
//
LOCUS       DATRNK010000121         2443 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.122, whole genome shotgun sequence.
ACCESSION   DATRNK010000121 DATRNK010000000
VERSION     DATRNK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.122"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..302
                     /locus_tag="VBC52_002019"
     CDS             <1..302
                     /locus_tag="VBC52_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HEO6883565.1"
                     /translation="ASPYNVKESILGVGILIQVVFFIYFLLKKINESPDERFYSNLAL
                     SASLMFLISIMLLILIAIYLNIYGTLELKSGYLYIMVGLLLLMFAVTYYFLEGRR"
     gene            305..523
                     /locus_tag="VBC52_002020"
     CDS             305..523
                     /locus_tag="VBC52_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6883566.1"
                     /translation="MGKETKIISSLKQVREDIGMTQQELAIRIGVRRETIGHLENNRY
                     NPSLEMALKIVKIFDMKIEDIFQLRKED"
     gene            1096..1656
                     /locus_tag="VBC52_002021"
     CDS             1096..1656
                     /locus_tag="VBC52_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6883567.1"
                     /translation="MNKFFLESERLIIRPLEESDYDSWYTSFMNRGPSLTPFDDGILD
                     MSMCDINWFKNLVNLQRKEWESDIVYIFGVFLKSGEHVGMLNIVTLARADMQWGELGY
                     VFHNQFWSNGYAFESILALLNSTYEKLGFHHIEAQITPGNERSEKLVRRLGLTYETTR
                     KDFSFENGKWTDKLIYSINLHNNNLE"
     gene            1756..2334
                     /locus_tag="VBC52_002022"
     CDS             1756..2334
                     /locus_tag="VBC52_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6883568.1"
                     /translation="MNNLDTLYCKLAMCNTVLTERLRLQPVELTNVNDFLEFSSDSET
                     VFYMQRYKANTVEEAQVVLANVCMKSPLGIYAMIEKESQKMIGIIELEIRDEFSAEFG
                     YILNKNYNGKGYMTEACSKLMSIGFEHLDLERIYARFDINNKKSGNVMERIGMKKEGE
                     LRHLAKNPKGEWKTRAYYSILKEEYFNKVNTK"
BASE COUNT          887 a          263 c          452 g          841 t
ORIGIN      
        1 cagcttcccc atacaatgtt aaagaatcaa ttcttggagt tggcatttta atacaagtag
       61 ttttcttcat ttattttcta ttaaaaaaaa ttaacgaaag tcccgatgaa cgcttttata
      121 gtaatttagc attgtcagct tctttgatgt ttttgatatc aataatgtta ttaattctta
      181 tagctattta ccttaatata tatggaacgt tagagttaaa atcaggctac ctttatatta
      241 tggtaggttt attattacta atgtttgctg ttacatatta ctttcttgag ggacggagat
      301 aataatgggg aaagaaacaa aaattatcag ttctttaaaa caggttagag aagatatcgg
      361 aatgactcaa caagagttag ctattcggat aggtgttagg agagaaacaa ttggtcattt
      421 agaaaataac aggtataatc cttctctaga gatggcactt aaaattgtta agattttcga
      481 catgaagata gaagatattt ttcaattaag gaaagaggat taaggtgaag gaagtaattt
      541 gttcgttttg tggggaaaag attttataac ttataaataa aataaggggt acgcaggatt
      601 gcttcctctt aataggatat tagctactgg cctagaagta catcatgata tttgtagaaa
      661 ttgtggtact attgctagaa gttatataaa tgacttggag aaattgaaat aggtatataa
      721 aatatattgg ttactcaaaa atcactaaaa tctcaaaatt tcttagtttt taacctgatt
      781 tccaaaacac aaatcttgtt aaatcagtgt ttgtttactt taaaaagtgc ttggtagcac
      841 tcaaaatcca gtgtcctcac ggacgttccg gttcgacccc ggccgcctgt atactttaaa
      901 agataaggtt tctagacctc gtcttttttg ttttgttaga taatttattg tgaaaagtta
      961 cctaaaatta cttatatttg ttttttatta ttgtattttt tataatgtag ttaataactt
     1021 ttcaaggagg taatattatg gaaattttaa ttgaaacaca cttatttatt tactctggaa
     1081 gaaaggaaat aaaaattgaa taaatttttt ttagaatcag agagacttat tataaggcca
     1141 ctagaagaaa gtgattatga tagttggtat actagtttta tgaatcgtgg accatcttta
     1201 actccatttg atgatgggat acttgatatg tctatgtgtg acattaattg gtttaaaaat
     1261 ttagtaaatt tacaaaggaa agaatgggaa agtgacatag tatatatatt tggtgttttt
     1321 ctaaaaagcg gtgagcacgt aggaatgtta aatattgtta cccttgcaag agcagatatg
     1381 caatggggag aattgggata tgtttttcat aatcaatttt ggagtaatgg ctatgcattt
     1441 gagtcaatct tggcattact taatagtact tatgaaaaat tgggttttca tcatattgaa
     1501 gcacagatta ctcctgggaa tgaacgatcg gaaaagttgg ttagaagatt gggattaacc
     1561 tatgagacaa ctcgaaaaga tttttcattt gagaatggaa aatggacaga taaactgatt
     1621 tatagtataa atttacataa taataattta gaataatttt atagacttac aaagaaattt
     1681 aaagcttata atattgtatt tttggagatt attgaagtgg tttatagata attaaggaag
     1741 gaatattgat tagaaatgaa taatttggat acactatatt gtaaattagc aatgtgcaac
     1801 acagtactaa cggaaagatt acgactccag cctgttgaat taactaatgt aaatgatttt
     1861 ttagaatttt catctgattc tgaaacagta ttctatatgc aacgttataa agctaatacc
     1921 gtagaagaag cacaagtcgt tttagcaaat gtttgtatga aatcaccatt aggaatttat
     1981 gcaatgattg aaaaagaatc acagaagatg ataggtatta ttgaattaga aatcagagat
     2041 gaattttctg cagaatttgg ttatatttta aataaaaatt ataatggaaa aggttatatg
     2101 acagaagctt gttcaaaatt gatgtcaatt ggatttgaac atctagactt ggaaagaatt
     2161 tatgctagat ttgacattaa taataaaaaa tctggaaatg ttatggaacg gataggtatg
     2221 aaaaaagaag gcgagcttcg tcatcttgca aaaaatccaa aaggtgagtg gaaaacaaga
     2281 gcgtattatt ctattttaaa agaagaatat tttaataaag ttaatactaa atagtctgag
     2341 atgtagagaa ttagatataa tctttttaat cagccagtgg gtgatgtcgt tgaaaatttt
     2401 acggaaggta aaaatccgaa gattgataca cttaatggga aaa
//
LOCUS       DATRNK010000122         2435 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.123, whole genome shotgun sequence.
ACCESSION   DATRNK010000122 DATRNK010000000
VERSION     DATRNK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.123"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            113..1120
                     /locus_tag="VBC52_002023"
     CDS             113..1120
                     /locus_tag="VBC52_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO6883569.1"
                     /translation="MRVHITSIYGQSPRSIALISQKLVKDVGRQLGYDEMGIYFYNDH
                     AETHGERSTRMDGIIAGLGRGDIVVFQVPTWNSTEFDELFLDKLQAYGARIITFVHDI
                     VPLMFESNFYLLDRVIDMYNRSDVVILPTKAMHDYLIEKGMTTSKVLYQEVWDHPVNI
                     DLPRPECQKVLSFAGDIQRFPFVNDWKENIPLIYYGDGSRLNSEANVHAQGWKDDVEL
                     MLSLSKRGGFGLCWSEDREELVERRYSRMNASYKLSTFLAAGLPIIANHDISSRDFIK
                     QHGLGFTVETLEEAVEKINNMEKETYDSYVENVEKIATLLRNGYITKKLLIDAVHMLY
                     R"
     gene            1129..2334
                     /locus_tag="VBC52_002024"
     CDS             1129..2334
                     /locus_tag="VBC52_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6883570.1"
                     /translation="MKKTIVLGADFQYRDQVMTTIKSIVSHNQHLTIYIINTDFPVEW
                     FNILNHSLEQFDCRVKNIPISSDVFEGIPTLSHISVAGFFRWFIPIHLEEEIVLYLDS
                     DVIVRGSLDPLFDINLEENLLGAVADHFSTLYYGDTAPVSFNSGVMLINNSLWKKEEI
                     YNSLMRIADKGSAVGVGDQEYLNILTQNRWIDIGKQYNVQIGQDVNINAYGRPDLYHF
                     YDDCEPVIVHYNSQDKPWNKYSQSRYRSEWWYYFGLEWSVIYAQQQKNLNRLTGKTLN
                     LFTLTASDTLEHIEVLAKALPDYQFHIAARSDISEKLANLETYSNIKVYKLVINPILE
                     ELIRNCDVYLDINHSYEVEGILDKIKEENKPILAFDSTAHTTKNIEVISETMPELMVK
                     RIREVGPLR"
     gene            2331..>2435
                     /locus_tag="VBC52_002025"
     CDS             2331..>2435
                     /locus_tag="VBC52_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEO6883571.1"
                     /translation="MKNRKAIALAADFGYQEQVKTIIKSICFHNQFIDF"
BASE COUNT          826 a          335 c          486 g          788 t
ORIGIN      
        1 taatgtaaaa aagctttgga tcattttagt acaatgagtg ttaataaaac tattatttaa
       61 ctggtatgat atggtctgga taagagagag ttttaaagag agattaacta aaatgcgagt
      121 acatattaca agtatatatg ggcagtcacc gcgtagcatt gcccttatat cacaaaaact
      181 agttaaggat gtcggacgtc aacttggtta tgatgaaatg ggaatctact tttataatga
      241 tcatgctgaa acgcatggtg aaagaagtac taggatggat ggtatcattg cgggattagg
      301 cagaggggat attgttgttt ttcaagtacc tacatggaat tcaacagaat ttgatgaact
      361 ttttctagat aaattacagg catacggtgc tagaattata acttttgtac atgatattgt
      421 tcccttaatg tttgagtcta atttttattt attagataga gtaattgaca tgtataatcg
      481 ttcggatgtt gttattctac ctacaaaagc gatgcatgat tatctaattg aaaaagggat
      541 gactacctct aaagttcttt atcaagaagt atgggatcat ccagttaata tagacctccc
      601 tcgtccagaa tgtcaaaaag tccttagttt tgcaggcgat attcaacgct ttccctttgt
      661 caatgactgg aaggaaaata tcccgctcat ctattatgga gacggaagca gactcaattc
      721 tgaggcaaat gttcatgctc agggatggaa agatgatgta gaattgatgc taagtctttc
      781 taagcgggga ggatttggcc tgtgttggag tgaagataga gaggagttag tcgagcgtcg
      841 ttattcgagg atgaatgcct cgtataaatt atctactttc ttggctgctg gacttccaat
      901 tattgctaac catgatattt caagtagaga ctttatcaaa caacacggat taggatttac
      961 agttgaaacg ctagaagaag ctgttgaaaa aattaataat atggaaaaag agacttatga
     1021 cagctatgtg gaaaatgttg agaagatagc aactctcttg cgaaatggtt atatcactaa
     1081 aaaattgttg atagatgctg tgcatatgct ttatagatag gggtagcgat gaagaaaaca
     1141 attgttttag gtgcagattt ccaatatcgt gatcaagtta tgacaactat aaaatctatt
     1201 gttagtcata atcaacattt aactatttat atcattaaca cagattttcc cgtagaatgg
     1261 tttaatatcc ttaaccattc tttagaacag tttgactgta gagtaaaaaa tattcccata
     1321 agttcagatg tctttgaggg gatacctacc ctgagccata tcagtgtggc gggatttttc
     1381 agatggttta ttccaattca ccttgaagaa gagatagttt tatatttaga tagtgatgta
     1441 atagttaggg gttctttgga tcctctattt gacataaacc ttgaagagaa tttattagga
     1501 gctgtagcag atcatttttc aacgctttac tatggagata cagcaccagt tagttttaat
     1561 tcaggtgtta tgctaatcaa taattcatta tggaaaaaag aagaaattta taatagcctt
     1621 atgaggattg ctgataaggg ttctgctgtt ggggtaggag atcaagaata ccttaatatt
     1681 ttgactcaga atcgttggat agatatcggt aaacaatata atgttcagat tggtcaagat
     1741 gtcaatatta atgcatatgg gcgaccagat ttgtatcatt tttatgatga ttgcgaacct
     1801 gttattgttc attataatag tcaagataaa ccgtggaata aatatagtca atcgcgatac
     1861 cgttcagaat ggtggtatta ttttggttta gaatggtcag ttatttatgc tcaacaacaa
     1921 aaaaatttga acaggttaac aggtaaaaca ctgaatttat ttacattaac agcctcagat
     1981 acattagaac atattgaagt acttgcaaaa gctctaccag attatcagtt tcatattgca
     2041 gcacgtagtg atatttcaga gaaattagct aatctagaaa cttatagcaa tattaaagtt
     2101 tataaattag ttatcaaccc tatcttggaa gagcttatac gtaactgtga tgtgtacttg
     2161 gatattaatc attcttacga agtagaaggt attctagata aaataaaaga agagaataaa
     2221 ccgattttag cctttgattc tacagcacat acgacaaaaa atatagaagt tatctcagaa
     2281 acaatgcctg aattgatggt taaaagaata agggaagtag gtcctcttag atgaaaaata
     2341 gaaaagctat cgcgttagcg gcagattttg gctatcaaga gcaagttaag actataatta
     2401 aatcaatatg ctttcataat caatttattg atttt
//
LOCUS       DATRNK010000123         2417 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.124, whole genome shotgun sequence.
ACCESSION   DATRNK010000123 DATRNK010000000
VERSION     DATRNK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.124"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            68..1150
                     /locus_tag="VBC52_002026"
     CDS             68..1150
                     /locus_tag="VBC52_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6883572.1"
                     /translation="MITKFPDQWQDKLTQRQFDDLTDIQNKLFQPITDGDNILGISPT
                     GTGKTLAYLFPTLLKLQPKKSQQLLILAPNSELAGQIFDVTKEWAEPLGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTAGRVFELVKLKKIKMMNINTIVLDEFDELLGDSQYHFV
                     DNIINRVPRDQQMIYISATNKLDNSKLADNTITIDLSNQKLDTIKHYYITVDKRERTD
                     LLRKFSNIPDFRGLVFFNSLSDLGACEERLQFNRASAVSLASDINIKFRKVILEKFKN
                     HDISLLLGTDLVARGIDIDNLEYVINFDIARDKETYTHRSGRTGRMGKEGCVITFVTH
                     KEELKQLKKYATVTELVLHNQKLHLK"
     gene            complement(1183..>2417)
                     /locus_tag="VBC52_002027"
     CDS             complement(1183..>2417)
                     /locus_tag="VBC52_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO6883573.1"
                     /translation="IFSLIIIAILAWQGFSFLKHKEIKLQQAVVEKEIRIAEKTVEVV
                     KRQKTERVLFLEPKGYDKSLSADILKWNQKSFEHKKFYDNQYIILRPQLADSNFANVK
                     KLSIYQILYQKEKGSMFQKSSRLLRTYLLDQNKKPFELDELLAHNISGFKAILENIAP
                     GTQLKEHDSNKEFLKTGRVTDGLDVKDGKLIINDLKLPLDKLYNVIDESYLKSSDLDL
                     VSNLKAKAPRVALTFDDGPNEKTTPKALEILKRYNAKATFFVMGQSAVGHTDILQRMH
                     AEGHEIGNHTWDHPNLTKLPAEKIKEEIHKTNDLIMKATGQKPVYLRPPYGATNATVK
                     TVTGLKEMLWSVDTEDWKNHNTQAMMTNIKKQLRPGGVILMHDIHQTTIDALPTIMDY
                     LTTQGYYFVTVGELYSTP"
BASE COUNT          800 a          428 c          368 g          821 t
ORIGIN      
        1 aacgctctaa aaataaaaag catgttataa taacaatgat aatatataaa ataacaagag
       61 gattcttatg ataacaaaat ttccagacca atggcaggat aaattaaccc agcgtcaatt
      121 tgatgatcta acagatattc aaaataaact ttttcaacct attactgatg gtgataatat
      181 ccttggaatt agccctacag gtactggtaa aactctagca taccttttcc caacgcttct
      241 taagttgcaa cctaaaaaga gccaacaact cttaatttta gctccaaatt cagaattagc
      301 tggacaaatc ttcgacgtta caaaagaatg ggctgaacca ttaggactga ccgcacaact
      361 attcctttca ggttctagtc aaaaacgcca aattgagcga ctaaaaaaag gaccagaaat
      421 tcttatagga actgctggac gcgtatttga acttgtgaaa ctaaaaaaaa taaaaatgat
      481 gaacattaat actatcgtcc ttgatgaatt cgatgaatta ctcggcgact cacaatatca
      541 ttttgtggac aatattatta atcgcgttcc tcgtgatcag caaatgattt acattagtgc
      601 caccaacaaa ctggacaata gtaaacttgc cgataacact ataacaatcg atctatctaa
      661 tcaaaaactt gatactataa aacattatta tattacagtt gataaaagag agcgtactga
      721 cctacttcgt aaattttcaa atattcctga ttttagaggt cttgtctttt ttaacagctt
      781 atcagactta ggtgcatgtg aagaacgttt acaatttaat agggcctcgg cagtttcttt
      841 agcaagcgat attaatatta aattccgtaa agttatctta gagaaattta aaaaccatga
      901 tatttcatta ctcctaggaa ctgatttggt agctcgtggc attgatatcg acaacctaga
      961 atatgtgatt aattttgata tcgcacgcga caaagaaaca tatacacatc gttctggacg
     1021 aactggtcgt atggggaaag agggatgtgt tattacattt gtaacacata aagaagagct
     1081 caaacaactt aaaaaatacg ccaccgttac agaacttgta cttcacaatc aaaaattaca
     1141 tttaaaataa aaaaagaaac aagtaacttg tttctttttt tattaaggcg ttgaatataa
     1201 ttcacctaca gtaacaaaat aatagccctg ggttgtcaga taatccataa ttgtaggcag
     1261 agcatctatg gttgtctggt gaatatcatg catcaaaatc actcctccag gacgtaattg
     1321 ttttttgata ttggtcatca ttgcttgtgt attatgattt ttccagtctt ctgtatcaac
     1381 tgaccacaac atttctttca atcctgtcac tgtttttaca gtagcatttg ttgctccgta
     1441 agggggacgt aagtacaccg gtttttgtcc tgttgctttc ataatcagat cattagtctt
     1501 atgaatctct tccttgattt tctccgctgg tagtttggtt aaattaggat gatcccatgt
     1561 atgattacca atctcatgac cttcagcatg catcctttgt aaaatatctg tgtgccctac
     1621 agcactttgt cccattacaa agaaagtcgc tttagcatta tatcttttaa ggatttctaa
     1681 ggctttagga gtcgtttttt catttggtcc atcatcaaag gtaagtgcaa cacgtggtgc
     1741 tttagctttt aaattagaaa ctaaatctag atcacttgat tttaaatatg actcatcgat
     1801 cacattatat aatttatcaa gtggtaattt taaatcatta ataattaact taccatcttt
     1861 aacatctagc ccatctgtta ctcttccagt ttttaaaaat tctttattgc tatcatgttc
     1921 ctttagttga gttcctggag ctatattttc taaaatagcc ttaaatccag atatattatg
     1981 agctaaaagc tcatctaatt caaaaggttt cttattttga tcaagtaagt aggttctcaa
     2041 aaggcgactt gatttttgaa acatacttcc tttttctttt tgatataaga tttgataaat
     2101 ggataatttt ttaacatttg caaagttaga atctgctaat tgaggacgta gaataatgta
     2161 ttgattgtca taaaattttt tatgctcaaa gcttttttga ttccacttta aaatatcagc
     2221 gcttagactt ttgtcatatc ctttaggttc aagaaaaaga acacgttcag ttttctgtct
     2281 tttcaccact tctaccgttt tctcggctat gcgaatttct ttttcaacaa cagcttgttg
     2341 aagttttatt tctttatgtt tcaaaaacga aaaaccttgc caagcaagaa tcgctataat
     2401 aattagacta aaaatac
//
LOCUS       DATRNK010000124         2342 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.125, whole genome shotgun sequence.
ACCESSION   DATRNK010000124 DATRNK010000000
VERSION     DATRNK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.125"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            456..1343
                     /locus_tag="VBC52_002028"
     CDS             456..1343
                     /locus_tag="VBC52_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6883574.1"
                     /translation="MKGIQAEAGVDAYIIEGEAKAGFKVFGLNIDARAIIAIAIAESS
                     LGTQGVATAPGANMFGFGAFDNNTTNAQNFSDDKAVIKMTQETIIQNQNTSFAIQDQK
                     AQLLSTGNLNVAARGGVYCTDASGSGKRRAAIMESIDKWIDAHGGISEISKELLNTSS
                     VAMMAVPTSYSVSRANQAGNYVAGTYPWGQCTWYVFNRTKELGYQFGPFMGNGGDWKN
                     KPGYQTTHEAKTGYAISFSSGQAGADRTYGHVAIVEDVKEDGSILISESNVLGLGTIS
                     YRTFSAAEAAQLTYIVGEK"
     gene            1739..2188
                     /locus_tag="VBC52_002029"
     CDS             1739..2188
                     /locus_tag="VBC52_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEO6883575.1"
                     /translation="MTEQQIEYINNRSEIAGHLEAIVSEIYLREESGKIESWFVIIPD
                     MNSFVAETNMDREQILFLLDSAHKYHIYFLFGGLASYLMTNISDVPKAIRTQAQYVLL
                     GMRVMDQSVLPKSYNSKEPYLKPDMIYIHDRRQERMLKITQEIEMEH"
BASE COUNT          812 a          408 c          454 g          668 t
ORIGIN      
        1 agtttatctt cgtaattata ttttatagat tcagcacaaa ttatttcaca aaattgaaga
       61 caacattgat tcatcgaaaa taaacgaaac tccacaggaa cttttgcttc ataactatag
      121 actttacatg cattcaatag gtccacattt aataaagaaa aacgttttga aaaaagataa
      181 atatattgta atgagtttat tttgagcaat taccacgcta tacttttaag ttgctacaaa
      241 cctatcgttt ggggtgtttt ttggtaaaat agaatgaaaa gagttatgga gataaaaaaa
      301 tggcacaacc attaatgctt taggaggaaa aacttcagta ggagttatga atcccttttc
      361 aacaagtaaa cataaaggta aaacaaaaaa caaagtttct tggagccgaa gcaaatgctg
      421 cagttggaca gttcactaat gaagacggaa aatcagtgaa agggattcaa gctgaagctg
      481 gcgtagatgc ttatattata gagggtgaag caaaagctgg atttaaagta tttggtttaa
      541 atattgatgc gcgtgcaatc attgccattg ctatcgcaga aagttcgctt ggtacacaag
      601 gtgttgccac agcaccaggt gctaatatgt ttggttttgg ggcatttgac aacaatacaa
      661 ctaatgctca aaactttagt gatgacaagg ctgtcattaa aatgacacag gaaaccatca
      721 tccaaaacca aaacaccagc tttgccattc aagaccaaaa agcacaactc ttgtcaactg
      781 gcaacttgaa tgtagcggct cgaggtgggg tttactgcac ggatgctagc ggttctggta
      841 aacgtcgtgc ggccattatg gagagcattg acaagtggat tgacgcgcat ggcggaattt
      901 ctgagatttc aaaggaactg ctcaacacgt cgtccgtcgc tatgatggca gtcccaacga
      961 gttactctgt ttcaagagct aatcaagcgg gcaactatgt ggcaggcact tacccttggg
     1021 gccaatgcac ttggtacgtc tttaaccgca ccaaagaact tggctaccag tttggaccat
     1081 ttatgggtaa tggtggcgat tggaaaaata agccgggcta tcaaaccaca cacgaggcaa
     1141 aaacaggcta cgccatctca tttagctccg gccaagctgg tgctgataga acttacggcc
     1201 acgtggccat cgtcgaagac gttaaggaag acggatctat cctgatctcc gaatcaaatg
     1261 tcctaggact tggcaccatc tcctaccgca ccttcagtgc agcagaagca gcacaattga
     1321 cttatatagt tggggagaag tagataatta ttctttgaaa aacaaaaggt tcttagcttg
     1381 tcaaaatcta agaccctttt atctttcata agaactttaa aatcaacaga tttttatcac
     1441 caaatgaact ggaccaagac caatagccat tccaattgtt ccagaaacac ttacctttga
     1501 ggagtttaag gcatatgagg atgttaaaaa agcaagtgag actaatcaga tttcacttgg
     1561 tattagctta aaagaagttg ataattgtag cattccactt tcgaaattca aacagttact
     1621 ttttacttct gacaatccag ataatttgtc taacttgacg cgcttcttag ttaaatctgt
     1681 ttcattgatt aaagatagta aggtttatct tttagacact gcttctgatt atgatgattt
     1741 gacagaacaa caaattgaat atatcaataa tcgaagtgaa atagcaggac atttagaagc
     1801 tattgtttca gaaatctatt taagagaaga atctggtaaa atagaatcat ggtttgttat
     1861 tattcctgat atgaatagtt ttgtggcaga aacaaatatg gatagagaac agattttatt
     1921 cttacttgac tcggctcaca aatatcatat ttatttctta tttggtggtt tagcatcata
     1981 ccttatgact aatatctcag atgttccaaa agctattaga acacaggcac agtatgttct
     2041 cttaggaatg agagttatgg atcagtcagt attaccgaaa tcatataatt caaaagaacc
     2101 ttatttgaaa ccagatatga tttatattca tgatagaaga caagaaagaa tgcttaaaat
     2161 cactcaagaa atagaaatgg agcattgagc atatgaaaaa acaaagacta ttactgcttt
     2221 ttggaggctt attaataatg ataatgatga cagcatgtaa ggattcaaaa atcccagaaa
     2281 accgcacgaa aaaggaatac caggcagaac agaattttaa gtcatacttt aaatatatat
     2341 ca
//
LOCUS       DATRNK010000125         2285 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.126, whole genome shotgun sequence.
ACCESSION   DATRNK010000125 DATRNK010000000
VERSION     DATRNK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.126"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..709)
                     /locus_tag="VBC52_002030"
     CDS             complement(<1..709)
                     /locus_tag="VBC52_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEO6883576.1"
                     /translation="MMKNFIKQSDTTFVRIIKSLLIGGFIGAILGSVGALFIIFGQDK
                     YLSEINIVQYFLWVSRIVVIITALFSLIYLYQIQKYQKVFFNVDESQSEEIYRQINLR
                     HSYGMTFVSISIVLSIVNTLFNYKLNIFDDSVTLVIPIYDLSLLFVLLGLHIYFLKVY
                     RNIRGIKMTVAPTLKELKNNVLQLDEAELESNYKMCFDIVMNLSGFIFPTIYFVLFFI
                     SFVFQKVEIVAIIITTSI"
     gene            complement(709..924)
                     /locus_tag="VBC52_002031"
     CDS             complement(709..924)
                     /locus_tag="VBC52_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6883577.1"
                     /translation="MGLILKNRLKELRARDGINQTELAKLTGVSRQTISLIERGEYTP
                     SVIIAMKISQVFKESLENVFSLVSMEE"
     gene            complement(969..1262)
                     /locus_tag="VBC52_002032"
     CDS             complement(969..1262)
                     /locus_tag="VBC52_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883578.1"
                     /translation="MVMTYIGVPKEQKALSRRGKQLEKINQGGKTLMSQMIQDLVGST
                     CMIYLEDGSQTHPYEILKVDEDWVQIRRNLKRSKEEVRIVRIHDIKELRPVNK"
     gene            complement(1295..1483)
                     /locus_tag="VBC52_002033"
     CDS             complement(1295..1483)
                     /locus_tag="VBC52_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883579.1"
                     /translation="MKKEFIITGRMVARVMETLVCLCMVSNLVLDMLDIIEINEIVFC
                     VLVASIAGLEFCLQKERE"
     gene            complement(1684..>2285)
                     /locus_tag="VBC52_002034"
     CDS             complement(1684..>2285)
                     /locus_tag="VBC52_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEO6883580.1"
                     /translation="TDRYPDTIYYRLLAHKFLPETLDRILYLDADMLCLNDFSSLYDM
                     ELGDQLYAAASHNTDGKFLDYVNKLRLKNVELESSYFNTGVLLMNLPAIRKVVHQQTI
                     LDYIMQNRGRLILPDQDILNGLYANLVKSIPDEIYNYDARYSLIYQLKSRNEWDLEWV
                     INHTVFLHFAGRDKPWKKDYRGRYSGLYKFMAKEALTKN"
BASE COUNT          802 a          416 c          283 g          784 t
ORIGIN      
        1 gaatagatgt tgttataata attgctacaa tctccacttt ttgaaaaaca aatgagataa
       61 agaataatac aaaatagatc gtagggaaaa taaaaccact taaattcatg acaatatcaa
      121 aacacatctt ataatttgat tccaactctg cttcgtccaa ttgtaagaca ttgtttttca
      181 actcttttaa agttggagcg actgtcattt ttatacctcg gatattccga tataccttta
      241 aaaaatatat atgtaaaccc aataaaacaa atagcagtga taaatcatat attggaataa
      301 ctaaagtaac tgaatcatca aaaatattta gtttatagtt aaataaagta ttaactatac
      361 tgagtactat tgaaattgac acaaaagtca tcccataaga atgtcttaaa ttaatctggc
      421 gataaatttc ttcagattga ctttcatcaa cattgaaaaa aactttctga tatttttgaa
      481 tctgatagag gtagatgaga ctaaagagag ctgttataat tacaacaata cgactaaccc
      541 ataggaaata ttgaacaata ttaatttcag ataagtactt atcttgacca aatatgataa
      601 aaagagctcc tacggaacca agtatggccc ctataaaacc accaattaaa agacttttga
      661 taattcgaac aaatgtagta tcactttgtt ttataaaatt tttcatcatt attcctccat
      721 tgacactaaa ctaaaaacat tctctaggga ttctttaaat acttgagaaa tcttcatagc
      781 aataataaca gatggcgtat attctccgcg ttcaattagg ctaattgttt gtcttgagac
      841 tcctgttaat ttagcgagtt ctgtttgatt gataccatct cgtgcacgca attctttgag
      901 tctatttttt aaaattaatc ccatcatttt tctcctttat aaaatgatat atagatttat
      961 tcctcgctct atttattgac tggacgcaat tccttgatat catgaatacg gacaatacgc
     1021 acctcctctt tacttcgttt caagttgcga cgaatctgta cccaatcctc atctaccttt
     1081 aaaatctcat aaggatgagt ttgagaacca tcctctaaat aaatcataca tgtgcttcct
     1141 actaaatctt gaatcatttg tgacataagt gtttttcctc cctgatttat tttttctaat
     1201 tgtttacccc tacgactgag tgccttttgc tctttaggta caccaatata cgtcatgacc
     1261 ataaaaagcc attaagccaa acaattccat ctatctactc cctttctttt tgcaaacaaa
     1321 actcaagccc tgcaattgat gctaccaaca cacaaaatac aatttcattt atttctatta
     1381 tatcaagcat atcaagaaca agattactta ccatacataa gcaaactagg gtttccataa
     1441 ctcttgcaac catccgacct gtaataataa attccttttt catttgagta actcctaaaa
     1501 ccattatgct tttgaaacaa tgattaattt gattgttcaa tgattaagtg attttaacac
     1561 gctcctcctt tgatattatt tttctttttt ataaataatt attagcaaag attattgata
     1621 tcattaaaat atcaccccaa aaaaagttac taaatagaaa taatttagta accttttaaa
     1681 tcttcaattt ttagttaaag cctctttagc cataaactta taaaggcccg agtaacgtcc
     1741 tctataatct tttttccatg gtttatctcg tcctgcaaaa tgtaaaaaaa cagtatggtt
     1801 aataacccac tctaaatccc attcattgcg actttttaat tgataaataa gactatacct
     1861 agcatcataa ttataaattt catcaggtat tgatttaacc aaattagcgt ataagccatt
     1921 taagatatcc tgatctggta agattagacg accacggttt tgcattatgt aatctaagat
     1981 agtttgttga tgaacaactt ttcgtatagc aggcaagttc atcaagagta cccctgtatt
     2041 gaaataagag gattctagct ctacattttt taaccgtaat ttattgacat aatctagaaa
     2101 cttaccatca gtattatgac ttgccgctgc atatagttga tcacctaact ccatatcata
     2161 taaacttgag aaatcattta agcaaagcat atctgcatct aaataaagta tcctatctaa
     2221 ggtctcaggt aaaaatttat gagccaaaag tcgataatat attgtatctg gatagcgatc
     2281 agttg
//
LOCUS       DATRNK010000126         2273 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.127, whole genome shotgun sequence.
ACCESSION   DATRNK010000126 DATRNK010000000
VERSION     DATRNK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.127"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            119..412
                     /locus_tag="VBC52_002035"
     CDS             119..412
                     /locus_tag="VBC52_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HEO6883581.1"
                     /translation="MLLPVGSIVYLVDGNQKLVILNRASIVDQSGQEVYFDYLGGIFP
                     EGLNMEQVYYFNEEDIDEVIFEGYHDEEEERVSKLIEKWKQKEGKNIPKGKTE"
     gene            843..1088
                     /locus_tag="VBC52_002036"
     CDS             843..1088
                     /locus_tag="VBC52_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883582.1"
                     /translation="MEFNAESSTVFGGLTEVQGAVSAGLNYVSERFGSFNGTFDTSGK
                     GLDWTKTIQTGMKKREVAKAEELKKKKAEESKRKYQQ"
     gene            1132..1875
                     /locus_tag="VBC52_002037"
     CDS             1132..1875
                     /locus_tag="VBC52_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883583.1"
                     /translation="MESDTASRISQVFALLPRNFSKKVLKSDGLWDILANMESKGASG
                     KKNVDSILNFLAKYEGYSNFGKSATKARTYLENLASPIKAGVKFGLEKIAGYENLSEM
                     VLKGGSKAESVGKFLGKSVKIIGKGATVLTIADISITGLSKGIDEYSKTKDIGKAVKK
                     GALSAVASVGPLEGATLGATVGGVPGAIIGGGIGFAIQGVKALDPKFFDHPVKSSQKI
                     ADNIGKSVNGVKNSVSKYIGGFGKVIGFG"
     gene            1881..>2273
                     /locus_tag="VBC52_002038"
     CDS             1881..>2273
                     /locus_tag="VBC52_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883584.1"
                     /translation="MQKTRTIYSANFEESLNLYDNGMSSLNNEEHLKIEKQTKNGVPS
                     VWFTLKSIILTILFPILLNFFIVTISITIHAEKPSDLTIPISDVKLLTMNLYLIMLVF
                     WFLLVLLGKRFKQAFILPYRYQFHVYSYM"
BASE COUNT          826 a          264 c          469 g          714 t
ORIGIN      
        1 tattgtaatt taatattatt tggatttatt ttctctaatt gtttgttact aatcaaattt
       61 atcgaatttc aaccatcatt taatgggaaa atttattaca ttaaatagga gaaaaaatat
      121 gttattacca gttggaagta ttgtatattt agttgatgga aaccagaaat tggtgatttt
      181 aaaccgtgct tcaatagtag atcaaagtgg gcaagaagtt tattttgatt atctgggcgg
      241 gatattccca gaaggcttaa atatggaaca agtgtattat tttaatgagg aagatattga
      301 tgaggtcatt tttgagggat atcatgatga ggaagaagaa agagtatcaa aacttattga
      361 aaaatggaaa caaaaagaag gaaaaaatat acccaaaggt aaaactgaat agctattcaa
      421 tgattagttc gaatttttag aaaatactaa actcaatata tatatagata gttgttttta
      481 agcaactatc tatttaggaa caaagatgaa agttaagtat cttctttgtt cttaagtagg
      541 tagttttaat caaagatggt gaagatgtag gaaatgcagc tgttaagacg ggtcatgttg
      601 caattggaat gggagcaact aaaggaggtg cattgattag tggactgcca ggaatgataa
      661 ttggtggagc aattggtttt gttggaagag ttgcaggttc gatagcattt gatgccattg
      721 agataataaa gaggaaattg ctgataccat aaatgatact ataagtgata catgggatag
      781 tttaggaaat ctcttaggag ttttctgact ttaaaggtaa cctatgttta aaaaaacgtc
      841 tcatggaatt caatgcggaa tcctcgacag tctttggtgg actaacagaa gttcagggtg
      901 ccgtttctgc aggccttaat tatgtctcag aacgttttgg aagtttcaat gggacatttg
      961 atacctcagg aaaaggattg gattggacaa aaacaatcca aacaggaatg aaaaaaagag
     1021 aagttgccaa agcagaagaa ctgaaaaaga aaaaagctga agaaagtaag agaaaatatc
     1081 aacaataacg aagtttcata tgaaaaccta tataatgagt tcaaaaatat tatggaaagt
     1141 gatactgcta gtagaattag ccaagtattt gctttattac cacggaactt ctcaaaaaaa
     1201 gtactaaaat ctgatggcct atgggatatt ttagcgaata tggaatcaaa aggagcttca
     1261 ggcaagaaaa atgtagattc aattttaaat tttcttgcaa aatatgaagg ttatagtaat
     1321 tttggtaaat ctgctactaa agcaagaacg tatttggaaa atttagcaag tcccataaaa
     1381 gcaggtgtaa aattcggttt agaaaaaata gctggttatg aaaatttgtc agaaatggta
     1441 ttaaaaggtg gatctaaggc tgaatctgta ggtaaatttt taggcaagtc agttaagata
     1501 ataggtaaag gagcaacagt tctaactata gcagacatta gcattacggg attatcaaag
     1561 ggaatagatg agtattcaaa aacaaaagat attggcaagg ccgtaaaaaa gggtgcctta
     1621 tctgcggtcg cgagtgtcgg tcccttagaa ggagcaacat tgggtgcgac tgttggagga
     1681 gtaccaggag caattattgg tggtggaata ggctttgcta ttcaaggagt aaaagcttta
     1741 gatcctaagt tttttgatca cccagttaaa agttcacaga aaattgctga taacataggt
     1801 aaatctgtaa atggggtgaa aaattcagtt tccaaatata taggtggttt tggtaaggta
     1861 ataggttttg ggtgaggata atgcaaaaaa ctagaacaat ttattctgca aattttgaag
     1921 aaagtcttaa tctttatgac aatggaatga gtagtcttaa caatgaagag cacttgaaaa
     1981 ttgagaaaca gacaaagaat ggagttccat cggtttggtt tactttaaaa tccataattt
     2041 taactatttt atttcctata ttattgaatt tctttattgt cactatttcc attactattc
     2101 atgcggaaaa acctagtgac ttaacaatac ctatttctga cgttaaacta ttaacaatga
     2161 atttatattt aataatgtta gttttttggt ttctcttagt gttattagga aaacgtttta
     2221 aacaagcatt tattttacca tataggtatc agtttcacgt ttattcatat atg
//
LOCUS       DATRNK010000127         2239 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.128, whole genome shotgun sequence.
ACCESSION   DATRNK010000127 DATRNK010000000
VERSION     DATRNK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.128"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            92..223
                     /locus_tag="VBC52_002039"
                     /pseudo
     CDS             92..223
                     /locus_tag="VBC52_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880383.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            236..1816
                     /locus_tag="VBC52_002040"
     CDS             236..1816
                     /locus_tag="VBC52_002040"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEO6883585.1"
                     /translation="MAEKITSLRQALWNSADQLRGQMDANDYKNYLLGLIFYKHLSDK
                     LLLAVCDNLEEHFNTFTDTQKIFEDAYQDEDLKDDLISVVTGDLGYFIEPTLTFEKLI
                     QDVYHNTFQLESLAQGFRDIEQSGEDFENLFKDIDLYSKKLGSTPQKQNQTIANVMKT
                     LNEIDFESVDGDTLGDAYEYLIGEFASESGKKAGEFYTPQAVSHLMTQIVFSGREDQK
                     GMTLYDPAMESGTLLLNAKKYSHQSDTVSYYGQEINTSTYNLARMNMMLHGVAIENQH
                     LSNADTLDADWPTDEPTNFDGVLMNPPYSLKWSATAGFLTDPRFSSYGVLAPKSKADF
                     AFLLHGFYHLKNTGTMAIVLPHGVLFRGAAEGKIRQKLLEQGAIDTIIGLPSNIFYNT
                     SIPTTIIILKKNRTNKDVFFIDASKEFDKGKNQNTMTDAHIKKILDAYKSRDNSDKFS
                     YLASFDEIIENDYNLNIPRYVDTFEEVPVKPLPELAKQLNDIDQEIAKTNAKLDQLMK
                     RLVGTTKEAQDELDAFRK"
BASE COUNT          814 a          396 c          357 g          672 t
ORIGIN      
        1 acagcaatca aaagtacttt aaacttcagg gagcaggatc atcacaatta aatatatcaa
       61 aaaaagtgta gaaaatttta catttctaat accaaatttt aacgaacaag aagccattgg
      121 tgacttcttc caaaccttag accaacaaat tactcaaacg gaagaaaaac taacagaact
      181 taaagcactc aaacaaacat tactcaatcg tttattcgta taagaaagga cgcacatggc
      241 agagaaaata acttcactac ggcaagcact atggaattcg gccgatcaat tacgtggtca
      301 aatggatgcc aatgactata aaaactatct tcttggactc atcttttata aacacttatc
      361 tgataaatta ctcttggctg tttgtgataa tttagaagaa cactttaata cctttacaga
      421 tactcaaaaa atctttgaag atgcctatca agatgaagac ctaaaagatg acctcatttc
      481 tgttgttact ggtgatttag gttactttat tgaaccgaca ctgacctttg aaaaactcat
      541 ccaagatgtt tatcacaata ctttccaatt agaatcatta gctcaaggtt ttcgcgatat
      601 tgaacaaagt ggtgaagatt ttgaaaactt atttaaagat attgatcttt actctaaaaa
      661 actaggttct actcctcaaa aacaaaacca aaccattgcc aatgtcatga aaacattaaa
      721 tgagattgac tttgagtcag ttgatgggga tactttaggc gatgcttatg agtacttgat
      781 tggtgaattt gccagtgaat cagggaaaaa agcaggtgag ttctataccc cacaagctgt
      841 ctctcactta atgactcaga ttgtcttttc aggtcgtgaa gatcaaaaag gaatgactct
      901 ctatgaccct gctatggaat ctggaacact ccttctaaat gctaaaaaat atagtcatca
      961 atcagatacc gtttcttact atggtcaaga aatcaatact tctacttata acctggctcg
     1021 catgaacatg atgcttcatg gcgttgccat cgaaaaccaa cacttaagta atgctgatac
     1081 cttggatgcg gactggccaa cagatgagcc aaccaatttt gatggggtgt tgatgaaccc
     1141 accttattct ttaaaatggt cagcgactgc tggtttctta acggatccac gtttttcaag
     1201 ttacggtgta ttagcaccaa aatcaaaagc tgactttgcc tttttacttc atggtttcta
     1261 tcatttaaaa aatacaggaa ctatggcaat tgtcttacca catggagttc ttttccgtgg
     1321 agctgcagaa ggtaaaattc gccaaaaact actcgaacaa ggggctatcg atactattat
     1381 cggacttcca tcaaatatct tttataatac aagcatccct acgactatta tcatattaaa
     1441 gaagaacaga accaacaaag atgtcttctt tatagatgct tcaaaagagt ttgataaggg
     1501 caaaaatcaa aacacaatga cagatgctca tatcaagaag attcttgatg cttataagag
     1561 tcgtgataat tctgataagt tttcatattt agcttcattt gatgaaatca ttgaaaatga
     1621 ctataactta aatatcccac gctatgtcga tacttttgag gaagtccctg ttaaaccact
     1681 tccagaatta gcgaaacaac tcaatgatat tgatcaagaa attgccaaaa caaatgccaa
     1741 actagatcaa ctcatgaaac gactagtcgg aacaactaag gaagctcaag atgaattgga
     1801 tgcttttaga aaataataat caggcatgtg tcaaacttcc caacagtcaa ttttgaagtt
     1861 gaatcaaaaa agatataacc ccttatttct cagatatgtt tcaaaaagtg aataaaacaa
     1921 acacctttat atatttttaa aaagcaacct agaattgagt tattaggtga aaatgatata
     1981 atgaatgtcg tacaaaaaaa gattgcatct gcttttgatc atttttaaga atagggcagt
     2041 gtaaggcaat tataaattaa caacaaggac taatcccttg atatgaaagg aatttattaa
     2101 ttattatgat gaatatgcaa gacttgttcc atgatttttt atgtcttgta atcatcagaa
     2161 atgttgattt taagcctttt aaattttgct aaattgaact aaaatcactt aaatttatta
     2221 caataagaca aaaaataga
//
LOCUS       DATRNK010000128         2150 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.129, whole genome shotgun sequence.
ACCESSION   DATRNK010000128 DATRNK010000000
VERSION     DATRNK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.129"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(476..682)
                     /locus_tag="VBC52_002041"
     CDS             complement(476..682)
                     /locus_tag="VBC52_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883586.1"
                     /translation="MGLLLKLTGNSESLVLQIYDIDTKEWEERISLEKVEEGFFVKSK
                     IANPEYHTNNMNLKILILTLICIP"
     gene            complement(1117..1647)
                     /locus_tag="VBC52_002042"
     CDS             complement(1117..1647)
                     /locus_tag="VBC52_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883587.1"
                     /translation="MSPSEKLDRRIDSSSDLSQYVYDFEKQYTEYMKKGYSEKNASIQ
                     ARRTLNEILQENNPGLRSSDAMYDKETGIAAIAVKDKNTGETYIAYAGTNKDADGATD
                     FISDANIGANNSAYLKRLGDKATSFYDRVQSNGDNITVTTDHSYGDFLASRVAIELIP
                     FFINFRKVNDSFHFCY"
     gene            complement(1644..>2150)
                     /locus_tag="VBC52_002043"
     CDS             complement(1644..>2150)
                     /locus_tag="VBC52_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883588.1"
                     /translation="DVQDKVSETTTCSYRLEKQKNQEFIGNFEHEVSESSQYSTEVKN
                     QIQYPIQYKDNSIRFTEKTPSERYDEFVFSSFDSSLLKKYKIYYYLLKHPETELKGVS
                     YKIPINSEIVAPFIKQLNIKNPKKSSISVTKTGSKEYYYTISIDTDSEIYSIFEGITL
                     EDVKEFSK"
BASE COUNT          683 a          377 c          292 g          798 t
ORIGIN      
        1 gaggtctaag taaagaattt aagcgttttg ataataaaaa agtgagaaca atagattctc
       61 actcacaatt agcccggtaa tttctcacac gatttggtac tgctttttta aaattggacg
      121 atggtgttat ttttaatatg agtagctgca ctcgaatccg attgctcaaa agcacttgtc
      181 gctccattga gatagctatt cagatcagaa ataactggtt gaatgttttg gctgacttct
      241 ttgataactt ttgatccagc ttctaagtat tttcaatgtt cttgaaccca ttggtgatct
      301 ttttttacta agccacatgc tgttaaaatc atcattaagc ttatccctcc cataataata
      361 ataatcaatt ttttaaatcc tttaaacatc acttttcctc ttatcttatt gattttttca
      421 attttattgc tgtcttgacg ttgctatcgc aaacaaccaa ctcaatgcaa tatagttatg
      481 gtatacatat caatgtaaga atcaagattt ttaaattcat attatttgta tgatattcag
      541 gattagctat ttttgatttc acaaaaaaac cttcttctac tttttctaga ctgattcttt
      601 cttcccactc cttcgtatca atatcatata tttgaagaac taaactctca gagtttccag
      661 tcaattttag taataatccc aaaaccaatc tccttcctac aaaccaacgg ataattttat
      721 ttcaactggt cctggtttta attttacttg tgcaatttcg cttgttatac caactattga
      781 tatcgccgat actcctattc ctgatgatgc gcacgcatca atagttaaac aaagtacttt
      841 aaatcctaca gagggtcatt ccatcaatta atggcatcat cgcgcttgaa gcatggcata
      901 ggttttggta ttagaaactg aatatgaatt ctattatcta ttaaaaaaat caatatcacc
      961 agagtattta taaaacataa cgataaaaaa tcaaaaaata tttttaccat ttcccttaat
     1021 ttcatgagat gttttttaaa tcataggtta cctttaaagc cagaaaactc ctaagagatt
     1081 tcatgaacta gctgtcactt tataagtgat cacaagctaa taacaaaagt gaaatgaatc
     1141 attaactttt ctaaagttaa taaagaatgg aattagctca attgccactc tagaagcgag
     1201 aaagtcacca taagaatgat ctgttgtgac agtaatatta tcgccattac tttgaacacg
     1261 atcataaaag ctagtagctt tgtctcctaa tcgttttaaa taagcagagt tattggcacc
     1321 gatatttgca tctgaaataa agtcggttgc cccatcagca tccttattag tacccgcata
     1381 agctatataa gtctcaccag tatttttatc ttttacagca atagctgcaa tacctgtttc
     1441 tttatcatac atggcgtcgc tagatctcaa tcctggatta ttttcttgta gtatttcatt
     1501 cagggttcgt cgtgcttgta tactcgcatt tttttcagaa taccccttct tcatatattc
     1561 tgtatattgc ttttcaaaat catatacata ctgggagagg tctgatgaag aatcaattct
     1621 tctatctaat ttctcacttg gactcattta gaaaactcct ttacatcctc taatgtaata
     1681 ccttcgaata tagaatatat ctcagaatca gtatcaatac tgattgtata ataatattct
     1741 ttacttcccg tttttgtaac cgaaatagat gattttttag gatttttaat atttaattgc
     1801 tttataaaag gggctacaat ttcagaattt ataggaatct tataggaaac accttttaat
     1861 tcagtttcgg gatgttttag taagtaataa tatattttat atttttttaa taatgaagaa
     1921 tcaaatgaac taaaaacaaa ctcatcataa cgttctgacg gtgttttttc agtaaaacga
     1981 attgaattat ctttatactg gattggatac tgtatttgat ttttaacttc ggttgaatat
     2041 tgactagatt cactaacttc atgttcaaaa ttaccaatga actcttgatt cttttgcttt
     2101 tctagtctat atgaacaagt tgttgtttca ctgactttat cttgtacatc
//
LOCUS       DATRNK010000129         1972 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.130, whole genome shotgun sequence.
ACCESSION   DATRNK010000129 DATRNK010000000
VERSION     DATRNK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.130"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..1126)
                     /locus_tag="VBC52_002044"
     CDS             complement(<1..1126)
                     /locus_tag="VBC52_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883589.1"
                     /translation="MTSKALIWSILQIDSARAYNSFLYYLQKIPWLGRKIPNRWFKTD
                     DIKGVVLFIRIIFFFLRGFGRSIGYVLYCYVLSFFVRSGMKQLNILPQEGFTLTLTLM
                     VITSLLIVINFYIKLIEPNDMQQTHMIRLFRIEPSRYFQSAELLEASSELFFRSLSFG
                     IFFHLNHFAFWHGLTFALFLAGSRLGIKVLCLPLYGRGKDQENPESLLNILFYLGIGI
                     GVFLSLFFLLLGKQFSPYPFFSWYTGIAGLIIWLVSYYRLKTYSKLNHLTYLTLTHET
                     MKEKIAKIDNIELLGIEMKDRDINVSELSPLLFENLSGISYLNAIFLKRMSRYLQRKI
                     LVRLAILSVLFGIELLFLLFFKEKINIPMNEHSFSKFLFLS"
     gene            complement(1116..1817)
                     /locus_tag="VBC52_002045"
     CDS             complement(1116..1817)
                     /locus_tag="VBC52_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6883590.1"
                     /translation="MELVIRDIHKRFQETEVLRGASYRFYSGKITGVLGRNGAGKTTL
                     FNILYGDLAADNGTICLLKDNHEYPLTDKDIGIVYSENYLPEFLTGYEFVKFYMDLHP
                     SDDLMTIDDYLDFMEIGQTERHRIIKGYSDGMKSKLSLICLMISKPKVILLDEPLTAV
                     DVVSSIAIKRLLLELSEDHIIILSTHIMALAEDLCDIVAVLDKGKLQTLDIDRKHEQF
                     EERLLQVLKGDEYDK"
BASE COUNT          711 a          363 c          282 g          616 t
ORIGIN      
        1 cactcaaaaa taggaatttt gaaaaactat gctcattcat cggaatattt atcttttctt
       61 taaaaaatag caggaaaagg agttctattc caaacagaac acttaaaata gctaaacgta
      121 ccaaaatttt tctttgcaag tatctactca tccgttttaa aaagatagcg tttaaatatg
      181 aaatacctga taaattttca aatagcaggg gagaaagttc actgacattt atatccctat
      241 ctttcatttc aatcccaagc agctctatat tatcaatctt tgcaattttc tccttcatcg
      301 tctcatgcgt tagggtaagg taggtcaagt gattaagttt agaataagtc tttaatcgat
      361 agtaactaac taaccatatg ataagtcctg caataccggt gtaccatgaa aaaaatggat
      421 atggagagaa ctgtttgcca agaagaagga aaaagagaga aaggaagact cctataccaa
      481 tccctaagta aaataaaata tttagtaaag actctggatt ctcttggtct ttacctctac
      541 catataaagg caaacagagc accttgattc caaggcgact acctgctaaa aataaagcaa
      601 aagtcaaacc atgccaaaaa gcaaagtgat ttaaatggaa aaatatgcca aagctcaaac
      661 ttctaaaaaa tagttcacta ctggcttcta aaagttcagc ggattggaaa tacctcgatg
      721 gctcaattct gaaaaggcga atcatatgag tttgttgcat atcattaggt tctataagtt
      781 ttatatagaa atttatgaca attaaaagag aggtaatcac catcaatgtt aaagttaggg
      841 taaaaccttc ttgcggcaaa atatttaact gcttcatgcc acttctaaca aaaaatgata
      901 aaacataaca atatagtacg taacctatgg aacgaccaaa acctcttaag aaaaagaaaa
      961 tgatacgaat aaatagtact acacctttta tatcatcggt tttaaaccat cggttaggga
     1021 tttttcttcc caaccagggg attttctgaa ggtaatataa aaaactatta taagctctag
     1081 cactatcaat ctgtaatata gaccatatta atgctttact tgtcatattc atctcccttc
     1141 aacacttgaa gaagacgctc ttcgaattgt tcatgtttac gatcaatgtc taatgtctgg
     1201 agttttcctt tgtctaatac agccacaata tcacatagat cttctgctaa ggccattata
     1261 tgagttgata atataataat atgatcctca cttaattcca acaaaaggcg ttttattgca
     1321 atacttgata caacatcaac tgcagtcagt ggctcatcta gtaaaattac ttttggctta
     1381 gaaatcatca ggcaaattaa tgagagctta ctcttcattc catcagaata tcctttgata
     1441 attctatgac gctctgtttg tcctatttcc ataaaatcta aataatcatc tattgtcatt
     1501 aaatcatctg aaggatgtaa atccatgtaa aattttacaa attcataccc tgttaaaaat
     1561 tctggaaggt agttttcgga ataaacaata ccaatatcct tatcggtaag aggatactcg
     1621 tgattatcct tcaataaaca aatggtcccg ttgtcagctg caagatcccc ataaagtata
     1681 ttaaataaag ttgttttccc agcaccattc ctacctaaga cccctgttat tttacctgaa
     1741 taaaatcggt aacttgctcc tctcaagacc tctgtttcct gaaaccgctt atgaatatct
     1801 ctaattacta actccataaa aactcctttg aaagtaaatc ttttatatca ttgtactatt
     1861 actgtagttt aagcgcaagt ttttcaaaac tagtgtaata aaaacaaaaa aatggataaa
     1921 tacattatcc atttagtagt tctataaacg ttgaagagca tccaacattt gc
//
LOCUS       DATRNK010000130         1953 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.131, whole genome shotgun sequence.
ACCESSION   DATRNK010000130 DATRNK010000000
VERSION     DATRNK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.131"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(36..1061)
                     /gene="trpS"
                     /locus_tag="VBC52_002046"
     CDS             complement(36..1061)
                     /gene="trpS"
                     /locus_tag="VBC52_002046"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEO6883591.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQNEGSYTLFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTLFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPAGFLVYPVAQAADITAFKANLVPVGTDQKPMIE
                     QTREIVRSFNHAYNCQVLVEPEGIYPENDAAGRLPGLDGNAKMSKSLNNGIFLADDMD
                     TVKKKVMSMYTDPNHIKVEEPGQIEGNMVFHYLDVFGRDEDQKEITAMKEHYQKGGLG
                     DVKTKRYLLDILERELSPIRERRLEYAKDMGQVYQMLQKGSEKAQAVAASTLDEVKSA
                     MGLNYFK"
     gene            complement(1169..1861)
                     /locus_tag="VBC52_002047"
     CDS             complement(1169..1861)
                     /locus_tag="VBC52_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO6883592.1"
                     /translation="MRRDDIIMPQDGRKMAVLLGFIGGFIDVYSHMQFHSLVATQTGN
                     IILMVANLGQNDFHFFITKLFSIFFFSIGFLVGIVIKERAVTAFWRLYALCPLLLSTM
                     FIPFVENVNHNIWVALLAFSTGILMLTFSGSRIESEPYTILMTSGNYRKMLQFWYEYI
                     VNRQRTAMQKRRAINYSLVVLAFIVGALVAAIVYDIFAYRAILGVTITLLIIMIHYTI
                     EIIKNDLTLHNV"
BASE COUNT          654 a          373 c          274 g          652 t
ORIGIN      
        1 agatatatct agctctcttt tattatagga atatattatt taaaatagtt caaacccatg
       61 gctgatttaa cctcatccaa agtagaggct gcaacagctt gagctttctc actacctttt
      121 tgtaacattt gataaacttg tcccatatct ttagcatatt cgaggcgacg ttctctaata
      181 ggtgataatt cacgctctaa gatatcaagt agataacgtt ttgttttaac atctccaagt
      241 ccaccttttt gatagtgttc tttcatagca gtaatctctt tttggtcttc atctcttcca
      301 aaaacatcaa ggtaatgaaa taccatatta ccttcgattt gtcctggttc ttctacttta
      361 atatggtttg gatcagtata catactcata acttttttct taactgtatc catatcatct
      421 gctaaaaaga ttccattatt taatgactta gacatttttg cattaccatc taaccctggc
      481 agacgacctg ctgcatcatt ttctggatag attccttctg gttcaactaa aacctgacaa
      541 ttataagcat gattgaaaga tctaactatt tcacgtgttt gttcaatcat tggtttttgg
      601 tcagtcccta caggaacaag gttagcttta aaagcggtta tatcagctgc ttgtgcaaca
      661 ggataaacta agaatcctgc tggaatagac tcaccaaaac ctttttgagc aatttctgtt
      721 tttacagttg gattacgttc caaacgtgca agtgaaacca agttcatata atacatactt
      781 aattctgcta actcaggaat ttgactttga atgaataaag tcgacttgtt tggatctagc
      841 ccaacagcta aataatcaag tgcaacatta ccaattgatt caacaatagt ttgcggatct
      901 ttagcatggt ctgtcaatgc ttgttggtct gctaaaaaga caaataaagt atagctaccc
      961 tcattttgaa gtagaactct attttttaag ctacctacat aatgtccaat atgcaacttt
     1021 cctgtcggtc tatcacctgt taagataatt ggtttcgtca tgtttactcc ttaatatacc
     1081 aaagatatca tttcatatag ttatcttatc atgtcacaag ccctattgca attcatctct
     1141 acaagaaaca ttcaccttat taagatgatt aaacattatg taacgttaaa tcgttcttaa
     1201 taatttcaat cgtataatga atcataataa ttaaaagcgt tatcgtcaca cctaaaatag
     1261 ctctatatgc aaagatatcg taaactattg cagctaccaa tgctccaaca ataaatgcta
     1321 gaactaccag tgaataattg atagctcgtc ttttttgcat agccgttctc tgacgattaa
     1381 caatatactc ataccaaaat tgtagcattt ttcgataatt accagaagtc attaagatag
     1441 tatatggttc agattcaatg cgacttcctg aaaatgtcaa cattaaaata cctgtgctaa
     1501 aggctaataa agctacccag atattgtggt ttacattttc aacaaaagga ataaacatag
     1561 tagacagtaa aaggggacat aatgcatata gacgccaaaa agctgttact gcacgttctt
     1621 taatcacaat accaaccaaa aaaccaattg aaaaaaagaa aatagaaaat aatttagtga
     1681 taaaaaagtg aaaatcattt tgtccgagat ttgccaccat caagataata ttacctgtct
     1741 gtgttgcaac aagggagtga aactgcatgt gactataaac gtcaataaaa ccaccaataa
     1801 aacccaataa tacagccatt tttcttccat cttgtggcat tataatatca tctctgcgca
     1861 ttatagcaaa ttctccttct tgtattatca tgttttctat tatataccaa taaaatcggc
     1921 attcaactta aaaatatcga gaaatttctt ttc
//
LOCUS       DATRNK010000131         1935 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.132, whole genome shotgun sequence.
ACCESSION   DATRNK010000131 DATRNK010000000
VERSION     DATRNK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.132"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            280..450
                     /locus_tag="VBC52_002048"
     CDS             280..450
                     /locus_tag="VBC52_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883593.1"
                     /translation="MDKELTPQEKANKKWAENNREHRTYLSKRSTARSFINKNATKED
                     LLELKQLIESKL"
     gene            complement(491..691)
                     /locus_tag="VBC52_002049"
     CDS             complement(491..691)
                     /locus_tag="VBC52_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6883594.1"
                     /translation="MSQEKLKSKLDQAKGGAKEGFGKITGDKELEAKGFVEKTIAKGK
                     ELADDAKDAVEEAVDAVKEKLK"
     gene            complement(801..1100)
                     /locus_tag="VBC52_002050"
     CDS             complement(801..1100)
                     /locus_tag="VBC52_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS-like domain-containing protein"
                     /protein_id="HEO6883595.1"
                     /translation="MVTLTVKELAKNFSNDNKAGEILFEQLKSYFYTDTVVTVSFAGI
                     SEVSSSFVNSAFINLLSYYDFNHIKSQLKIVNSTKQINDLIKQRFSFEISRQITV"
     gene            complement(1094..>1935)
                     /locus_tag="VBC52_002051"
     CDS             complement(1094..>1935)
                     /locus_tag="VBC52_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6883596.1"
                     /translation="TNFNRLLALSDNRTLTVDMRNIEFAEPSGVISLYNMLTFATKRK
                     DANIKWLICEESSLNKRQRQAMLYLVDCGFFKVFDKLVYKEPELRPTTFEIKFINTEQ
                     IAQWKVTDFKNWLQKQTGRTNEFSSICVAVDEIFNNIADHSKESKGCIFGQYYPKNKE
                     IVIAVSDFGIGIPQSIKRKFKKDEPDNKLIEFALQEGVSAETIPQNRGAGLSNIVNTL
                     TTNKVGNFTIISNCGIVSVSDNKITQSYSSEESYPGTFFEIRIDVSNDNLYDLEEEEE
                     FEW"
BASE COUNT          642 a          348 c          272 g          673 t
ORIGIN      
        1 tatagaaagg aggaagatat gagaatatca gaaattgctg atttgcttac ttcaatcgga
       61 actctgttgg ttggtatagc aagcataatc acagcaataa aaaaagaacc taaaaagaaa
      121 aaccggccac ggagattcaa ataaggttct agtagtagtt tggggctcaa gccccttgcc
      181 actactgata gtatatcata tctaagacaa atatgaaata tttgattatt ttcgcaattt
      241 gtttagttgt attttacttt attaacaagg atgattgaaa tggataaaga attaacacct
      301 caagaaaaag caaataaaaa gtgggcagaa aacaatagag aacatagaac ctatctatca
      361 aaacgatcta ctgctcgtag ttttattaac aaaaatgcta caaaagaaga cttattagaa
      421 ttaaaacaat taattgaaag caaactctag acacacaaaa aaaccgccct caatagagag
      481 cggttaatat ttatttcagt ttttctttga cagcatctac tgcctcttca acagcatctt
      541 tagcatcatc tgctagttct ttgcctttag caattgtttt ttcaacaaat ccttttgctt
      601 ctaactcttt atcaccggtt attttgccaa agccttcttt agcaccacct tttgcttgat
      661 ctaattttga ttttagtttt tcttgtgaca taatgtgcct ccttattatt tttattctat
      721 ggtaacattt tgattatttc taggcaaata aaaaagcaag aaccgctagt gtcaggcgat
      781 tcttgctaaa taatatgcac ctatactgta atctgcctac ttatttcaaa actaaatcgt
      841 tgttttatta aatcatttat ttgttttgtt gaatttacaa tttttagttg acttttaata
      901 tgattaaaat catagtaaga caataaattg ataaaagcag agtttacaaa ggacgaactt
      961 acttcgctaa ttcctgcgaa cgaaacagtc acgactgtat ctgtataaaa ataagatttc
     1021 aattgttcaa ataaaatctc tcccgcctta ttatcgttag aaaaattctt tgctagttct
     1081 ttgacagtta acgttaccat tcgaattctt cctcctcttc taaatcatat aaattgtcat
     1141 tcgatacatc tattcgaatt tcaaaaaaag taccaggata tgattcctca gacgaataac
     1201 tttgagtaat tttattatct gataccgaga ctataccaca attagatata attgtaaagt
     1261 tcccgacttt gttagtagtt aaagtattta caatattaga aagccctgcc cctctatttt
     1321 gaggtatagt ttctgcagaa acaccctctt gaagagcgaa ttctattaac ttgttgtcag
     1381 gctcatcctt tttaaatttt cgctttatag actgagggat tcctattccg aaatcagata
     1441 ctgctattac aatctccttg ttcttgggat agtattgccc aaaaatacat cccttagatt
     1501 ccttagaatg atctgcaata ttattaaaaa tttcgtcaac cgctacacaa atagaactaa
     1561 actcatttgt tctgccagtt tgcttttgta accaattctt aaagtctgtt accttccatt
     1621 gagctatttg ttcagtgtta ataaatttaa tttcaaaagt agtcgggcgc agttccggct
     1681 ctttataaac caatttatca aacactttaa aaaagccaca atctactaga tacaacatag
     1741 cttgcctttg acgtttattt aaagagcttt cttcgcatat taaccacttg atgtttgcat
     1801 cttttctttt tgtagcaaaa gttaacatat tatataacga aattactcca ctaggttccg
     1861 caaactcaat gtttctcata tctactgtaa gtgtcctgtt atcacttaaa gcaagtaatc
     1921 tattgaaatt ggtaa
//
LOCUS       DATRNK010000132         1924 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.133, whole genome shotgun sequence.
ACCESSION   DATRNK010000132 DATRNK010000000
VERSION     DATRNK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.133"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..149
                     /locus_tag="VBC52_002052"
     CDS             <1..149
                     /locus_tag="VBC52_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001920755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6883597.1"
                     /translation="ALSSIANNKDRGNKLPTTGEDENPFFNIAALTIIASVGLLSILK
                     KKED"
     gene            462..1680
                     /locus_tag="VBC52_002053"
                     /pseudo
     CDS             462..1680
                     /locus_tag="VBC52_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740329.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
BASE COUNT          650 a          288 c          359 g          627 t
ORIGIN      
        1 aagctttatc aagtatcgca aataataaag atagaggaaa taaattgcct acaacaggtg
       61 aggatgagaa cccattcttt aacattgctg ctttgacaat tatagcttca gttggtttac
      121 tatctatttt aaagaaaaaa gaggattaat cgtttgacct aaaatgtcat taaacttttc
      181 gccatttatt ggtgtgagca ctttaacaaa gttatctctc tctccaactt aattaactaa
      241 agtgtttcaa tttttcgaga ttaattcttg aaaaagtcta tcgagattat gaatctcgat
      301 agatttttga ttttgtatgt taaacaataa agggtaagta ttctagaaat atttggtaag
      361 catctaataa gtttaaaata tagcaaatat ttaaaagaat tgtttttata atcttcgtcg
      421 cgtataatgg cacgtagaaa gttaattttg gaggtaattt aatgtcagtt caaagtgtgc
      481 ttgagtcctt gataatgatt aaagagtggc aagaggaaac ttacaaatat tttcatagcc
      541 atcccgaatt atcaatgcaa gagaaaaata ctagtcaatt tatttataaa accttgctgt
      601 cttatggtta ccaaactcta cgaattggag gaggcgttgt tggtatttta gaaaatggag
      661 atggtcctaa agtactattt agggcggata tggatgctct gcctgtaaaa gaagaaacag
      721 gattggaata tgcttctaaa gtagtcatga aagacttaga tggtaagaca gtacctgtga
      781 tgcatgcctg tggtcatgat gttcatattg tagctggctt aggggcagct tgggctttgg
      841 ctaatcattt atcggagtgg caaggaactt atattgcttt gtttcaacca ggtgaagaaa
      901 tagctgcagg ggctaaatct atgataaaag atggactttt tgaaaagata cctcatccag
      961 acttagcttt atcacaacat gttttaactg aacccatttc aggtaaagta ggtacgctag
     1021 cagggccctt tttatcgagt gccacttcat taaagattac tgtgtatgga aaaggtgctc
     1081 atagttcaat gccacattta agtattgatc ctattattat agcatgtgat attgtaataa
     1141 aactacaaac aatagtttca agagaagtaa atctatttaa atttgctgtt gtatctgttg
     1201 gttcttttca atctggtttt aaagcgaata ttattcctga caaagcaata ttattattga
     1261 atattagagc ttacgatgaa cagattcaaa aacaagtaat tgatagtatt aaacgtattg
     1321 ttaaatcaga atgtgaagta ggtggttgca taattgaccc caaagtagaa acttacgatg
     1381 agtatcccct aacagataat gacccccaga taaccaagga agtcacggaa gcatttcaga
     1441 aatatttagg tgaggataga tagggtttct gcctaccatc caatgactgc ttcggaagat
     1501 ttttcatata ttcctagagc attgggaatc ccttatcttt attggggatt cggaggtttc
     1561 acgaaagaac aagagattta ttcaaaccat aattctaagt ttgcaccagc tattcaaccg
     1621 accttaacaa ctggtactga agctgcagtt gtggctattt tacggtatct tggcaaatag
     1681 agaataatta catattaagt aaaagcaagg tatactaaaa tctccaaagt ctgaattggc
     1741 tttgacagtc ttttggataa gtaagtactc gataacaaga tatatattct ttcctctatt
     1801 tctccatcat attataagta ataacataaa tagagcttgc tctaatgatc aaacaatcat
     1861 taaaagcgtt tacaaaaaat ggtataatac tcttactaca ttcaagtgag gttcagtcat
     1921 gttc
//
LOCUS       DATRNK010000133         1886 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.134, whole genome shotgun sequence.
ACCESSION   DATRNK010000133 DATRNK010000000
VERSION     DATRNK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.134"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            22..573
                     /locus_tag="VBC52_002054"
     CDS             22..573
                     /locus_tag="VBC52_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001874207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6883598.1"
                     /translation="MSTEDSLTYISFSKFNSKNEFDVFFQTLLKSEDPYYLAIVDNNT
                     GKVLGTFSLMRIDTKNRVVEMGWVVYSSKLKQTRIATEAQYLVMKYVFEELCYRRYEW
                     KCDSLNAPSNNSAKRLGFTFEGTFRQAVVYKGRNRDTNWYSILDKEWPEKKTRFEKWL
                     DDSNFAVNGYQIRSLSSIEQKTK"
     gene            complement(833..1780)
                     /locus_tag="VBC52_002055"
     CDS             complement(833..1780)
                     /locus_tag="VBC52_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor regulator N-terminal
                     domain-containing protein"
                     /protein_id="HEO6883599.1"
                     /translation="MIKTKFSDPLEQLAKKRKRKTLFKTVVLALITVLLVVGIAFKGF
                     TYLTSKNGQKTYHNFELLSEIAYPNISYDSLYYQPSGQFTGKVHADRFKDIDGVQIPY
                     SSFEENYSISGTFGTNADEKSEKNGLYDRGTRQKVPQFFNKNVKYKKGEAKTTPTNDL
                     KYVNKLNNDLIEIAITFDKSYSYKEIKTMIPNNIKQNWLWIGTSTELDTSYWPTKYQF
                     GTDPETLNTPQIFIDKIKENLKGNSKVYFNNINIYSDLEKYAKKYDKVNNSDKLKFSG
                     IILTGKAENFKQLKEKEWVHASSIGANIEYKPYYKLDKE"
     gene            complement(1777..>1886)
                     /locus_tag="VBC52_002056"
     CDS             complement(1777..>1886)
                     /locus_tag="VBC52_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HEO6883600.1"
                     /translation="EISEITGQSQSTIKIRLMRARREVKKYLEKDNFKI"
BASE COUNT          586 a          263 c          278 g          759 t
ORIGIN      
        1 ttatttcaag tttatggaga attgtctact gaagatagtt taacttatat atcttttagc
       61 aaatttaata gtaagaatga atttgatgtc tttttccaaa ctctattaaa atcagaagat
      121 ccttattatt tagctattgt tgataataat actggtaaag tattagggac tttttcatta
      181 atgcgtattg atacaaaaaa tagagttgtt gaaatgggat gggtggttta ttcatcaaaa
      241 ctaaaacaaa caaggatagc aacagaagct caatatttgg ttatgaaata tgtttttgaa
      301 gaactctgtt accgccgtta tgagtggaaa tgtgatagtt tgaatgcacc atctaataat
      361 tctgcaaaac gacttggttt tacttttgaa ggaactttta gacaagctgt cgtatataag
      421 ggtcgtaaca gagatacaaa ttggtattct attttagata aagagtggcc agagaaaaaa
      481 acacggtttg aaaaatggct agatgattca aactttgctg taaatgggta tcaaatcaga
      541 tcactatcaa gcattgagca gaagacaaaa tagagtcatt cattaatcac taaaatttca
      601 aaattacatg tttttttagc taacatctta aactttgtta aataaatgtt tattaacttt
      661 taaaggtgca tggtaggact caaaatcggg tgtcctcacg gacgtgccag ctcgactttg
      721 gtcgcgggca tcaaagaaaa aagtcaagat tattatcttg tctttttttc tatgagttat
      781 taactgtaaa agttacataa aattaccaaa taaatttttt aacaattaat tatcattctt
      841 tatctaattt ataatagggc ttatattcta tgtttgcccc tatacttgaa gcatgtaccc
      901 attctttttc tttaagttgt ttaaagtttt cggctttacc tgttaatatt attcccgaaa
      961 attttaattt gtcgctatta tttaccttgt cgtatttttt ggcatacttt tctaaatctg
     1021 agtagatatt tatattatta aagtatactt ttgagttacc ttttaagttt tcttttattt
     1081 tatctatgaa tatttgtggt gtattcaaag tttcaggatc tgtaccgaat tgatactttg
     1141 ttggccaata tgatgtatct aattctgtgc ttgtacctat ccataaccaa ttttgtttta
     1201 tattatttgg tatcattgtt tttatttctt tgtaactata ggacttatca aaagttattg
     1261 caatttcaat aagatcatta ttcaatttat ttacatattt taaatcattt gttggagttg
     1321 ttttggcttc tcctttttta tacttaacat ttttattgaa aaattgtgga actttttgtc
     1381 tagtacctcg atcatacaag ccattctttt ctgatttttc atcagcattt gtgccaaaag
     1441 tacctgagat actataattt tcttcaaaac tagaatatgg aatttgaacc ccatcgatat
     1501 ctttaaatct atcagcatgt actttaccag taaactgacc gcttggctgg taatagagac
     1561 tatcatagga aatatttgga tatgctattt cagaaagtag ttcaaagtta tgatatgttt
     1621 tttgaccatt ttttgaagtt aaatatgtaa atcctttaaa agctattcca actactaata
     1681 gtacagttat taatgccaat actacggtct tgaataatgt tttccttttc ctttttttgg
     1741 ctaattgttc cagtggatca ctaaactttg tttttatcat attttaaaat tatccttttc
     1801 taaatatttt ttaacttctc ttctagctcg cataagtctt attttaattg tactttgtga
     1861 ttgtccagta atttcagata tttctt
//
LOCUS       DATRNK010000134         1782 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.135, whole genome shotgun sequence.
ACCESSION   DATRNK010000134 DATRNK010000000
VERSION     DATRNK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.135"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..306
                     /locus_tag="VBC52_002057"
     CDS             <1..306
                     /locus_tag="VBC52_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEO6883601.1"
                     /translation="ILVTQLAINCLIISIDHSCFEHSHYLIDKVRSLLQDEVNFYEKT
                     VFLYVTGYYHLKLGDTSSGKEDMRKALQIFKYLGEDSFYNTYNEHYCQKVLGNNKEC"
     gene            455..1276
                     /gene="mutM"
                     /locus_tag="VBC52_002058"
     CDS             455..1276
                     /gene="mutM"
                     /locus_tag="VBC52_002058"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEO6883602.1"
                     /translation="MPELPEVETVRKGLERLVVNQEIASITIKVPKMVKTDLNDFMIS
                     LPGKTIQQVLRRGKYLLFDFGEMVMVSHLRMEGKYLLFPNKVPDNKHFHLYFKLTNGS
                     TLVYQDVRKFGTFELVRKSSLKDYFTQKKLGPEPTADTFQFEPFSKGLANSKKPIKPL
                     LLDQRLVAGLGNIYVDEVLWAAKIHPQRLANQLTESETSLLHKEIIRILTLGIEKGGS
                     TIRTYKNALGEDGTMQKYLQVYGKTGQPCPRCGCLIKKIKVGGRGTHYCPRCQCL"
     gene            1267..>1782
                     /locus_tag="VBC52_002059"
     CDS             1267..>1782
                     /locus_tag="VBC52_002059"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEO6883603.1"
                     /translation="MLMTKIIGLTGGIASGKSTVTKIIRESGFKVIDADQVVHKLQAK
                     GGKLYQALLEWLGPEILDADGELDRPKLSQMIFANPDNMKTSARLQNSIIRQELACQR
                     DQLKQTEEIFFMDIPLLIEEKYIKWFDEIWLVFVDKEKQLQRLMARNNYSREEAELRL
                     SHQIPLTDKKSF"
BASE COUNT          644 a          261 c          345 g          532 t
ORIGIN      
        1 attctagtta cacaattagc aattaattgt ctaatcatca gcattgatca ctcttgcttt
       61 gagcatagcc actacttaat tgataaggtt aggtcattgt tacaggatga agttaatttc
      121 tatgaaaaaa ctgttttttt atatgtaact ggatactatc atttaaaact tggtgatact
      181 tcttcaggaa aagaagatat gaggaaagct ttacagattt ttaaatattt aggtgaagat
      241 tcattttaca atacttataa tgaacattat tgtcaaaaag tacttggcaa taataaagaa
      301 tgctaatgag tgcaagttta tgagtaattt ttaaagcttt tgtctcaaaa tgataataag
      361 aaaggaaaat agaaatcgaa tggtcaggtg acaccaagaa cgtttggaaa ttatttttct
      421 aagcaatgac tattgatttt tagactatat atttatgcca gaattaccag aagtagaaac
      481 cgttagaaaa ggactagaaa gacttgtagt taatcaagaa atagcttcta taacgattaa
      541 agtccctaaa atggtgaaaa ctgatttgaa tgactttatg ataagtctac caggaaaaac
      601 aattcaacag gtattgagac gagggaagta tcttcttttt gattttggtg aaatggttat
      661 ggtatcacat ttacgcatgg aaggaaaata tctactcttc ccaaataaag taccagataa
      721 caaacatttt cacctttatt tcaaattgac taatggcagc acattggttt accaagatgt
      781 ccgtaaattt gggacatttg agttggttcg taaatcatct ctcaaagatt attttactca
      841 aaaaaaatta ggtcctgagc caactgctga cacatttcaa tttgagcctt tttcaaaagg
      901 cttagcaaac tccaaaaagc caataaaacc tttactatta gatcagagat tagtagcagg
      961 acttggaaat atttacgtag atgaagtctt gtgggcagca aaaattcacc cacagcgttt
     1021 ggctaatcaa ttgaccgaat ctgaaacaag cttattacac aaggaaatta ttcgcatttt
     1081 aactttaggc atcgaaaaag gtggctccac gattagaaca tataagaatg ctcttggaga
     1141 ggacggtacg atgcaaaagt atttacaggt ctatggtaaa actgggcaac cgtgtccaag
     1201 gtgtggttgt ttaattaaga agataaaagt aggagggaga gggacgcatt attgccctcg
     1261 gtgtcaatgc ttatgacaaa aataatagga ctgacaggag ggatagcttc tggaaagtca
     1321 acggtaacaa aaataatacg agaatcaggt tttaaagtca tagatgcgga tcaagtggtt
     1381 cataaattgc aagctaaggg tgggaaactt taccaagctt tattagaatg gttgggtccc
     1441 gagatacttg atgctgatgg tgagttggat agaccaaagc tttctcaaat gatttttgct
     1501 aatccagaca atatgaagac atcagctagg ctacaaaata gtatcattcg tcaagagtta
     1561 gcatgtcagc gcgaccaatt aaaacaaaca gaagagatat ttttcatgga tattccttta
     1621 ttgattgaag aaaagtatat aaaatggttt gatgagattt ggttggtatt tgttgataaa
     1681 gaaaaacaat tacaacgatt aatggcccgt aacaactaca gtcgagaaga agcggaatta
     1741 cgactttcac accaaatacc tttaacagat aaaaaaagtt tc
//
LOCUS       DATRNK010000135         1737 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.136, whole genome shotgun sequence.
ACCESSION   DATRNK010000135 DATRNK010000000
VERSION     DATRNK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.136"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..574
                     /locus_tag="VBC52_002060"
     CDS             <1..574
                     /locus_tag="VBC52_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO6883604.1"
                     /translation="DITGAIIGLLICGIVAIFLVPQIRKDGGPAIFSQNRVGRNGRIF
                     RFYKFRSMRVDAEQIKKDLLVHNQMTGLMFKLDDDPRITKIGKFIRKTSIDELPQFYN
                     VLKGDMSLVGTRPPTVDEYEKYNSTQKRRLSFKPGITGLWQISGRNNITDFDEIVKLD
                     VQYINEWSIWSDIKIILLTLKVVLLGTGAK"
     gene            598..1047
                     /locus_tag="VBC52_002061"
     CDS             598..1047
                     /locus_tag="VBC52_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HEO6883605.1"
                     /translation="MKICLVGSSGGHLAHLNLLKPIWEKEDRFWVTFDKEDARSILRE
                     EIVYHCFFPTNRNVKNLVKNTILAFKVLRKERPDVIISSGAAVAVPFFYIGKLFGCKT
                     VYIEVFDRMDKPTLTGKLVYPVTDKFIVQWEEMKKVYPKAINLGGIF"
     gene            1047..1538
                     /locus_tag="VBC52_002062"
     CDS             1047..1538
                     /locus_tag="VBC52_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug MFS transporter"
                     /protein_id="HEO6883606.1"
                     /translation="MIFVTVGTHEQQFNRLIKEVDRLKGTGAIDQEVFIQTGYSDFEP
                     QNCQWSKFLSYDDMNSYMKEAEIVITHGGPATFMNAVSKGKKTIVVPRQEQFGEHVNN
                     HQVDFVNKVKTMYNFDIVVDIERLQNVVYEGMMNRPFLETNSSNFIEEFKVILKELCD
                     ENQ"
BASE COUNT          595 a          220 c          357 g          565 t
ORIGIN      
        1 ggatatcacg ggtgctatta taggtttgct catatgtggc attgtggcaa tttttctagt
       61 tccacaaatc agaaaagatg gtggaccggc tatcttttct caaaatagag taggtcgtaa
      121 tggtaggatt tttagattct ataaattcag atcaatgcga gtagatgcag aacaaattaa
      181 gaaagattta ttagttcaca atcaaatgac agggctaatg tttaagttag acgatgatcc
      241 tagaattact aaaataggaa aatttattcg aaaaacaagc atagatgagt tgcctcaatt
      301 ctataatgtt ttaaagggtg atatgagttt agtaggaaca cgccctccca cagttgatga
      361 atatgaaaag tataattcaa cgcagaagcg acgccttagt tttaagccag gaatcactgg
      421 tttgtggcaa atatctggta gaaataatat tactgatttt gatgaaatcg taaagttaga
      481 tgttcaatat atcaatgaat ggtctatttg gtcagatatt aagattattc tcctaacact
      541 aaaggtagtt ttactcggga caggagctaa gtaaaggtaa ggtttgaaag gaatataatg
      601 aaaatttgtc tggttggttc aagtggtggt catctagcac acttgaacct tttgaaaccc
      661 atttgggaaa aagaagatag gttttgggta acctttgata aagaagatgc taggagtatt
      721 ctaagagaag agattgtata tcattgcttc tttccaacaa accgtaatgt caaaaacttg
      781 gtaaaaaata ctattctagc ttttaaggtc cttagaaaag aaagaccaga tgttatcata
      841 tcatctggtg ccgctgtagc agtaccattc ttttatattg gtaagttatt tggttgtaag
      901 accgtttaca tagaggtttt cgacaggatg gataaaccaa ctttgacagg aaaattagtg
      961 tatcctgtaa cagataaatt tattgttcag tgggaagaaa tgaaaaaagt ttatcctaag
     1021 gcaattaatt taggaggaat tttttaatga tttttgtcac agtggggaca catgaacagc
     1081 agttcaaccg tcttattaaa gaagttgata gattaaaagg gacaggtgct attgatcaag
     1141 aagtgttcat tcaaacgggt tactcagact ttgaacctca gaattgtcag tggtcaaaat
     1201 ttctctcata tgatgatatg aactcttaca tgaaagaagc tgagattgtt atcacacatg
     1261 gcggcccagc gacgtttatg aatgcagttt ctaaaggaaa aaaaactatt gtggttccta
     1321 gacaagaaca gtttggagag catgtgaata atcatcaggt ggactttgtt aataaggtaa
     1381 aaacaatgta taattttgat atcgttgtag atattgaaag gttacaaaat gtagtctatg
     1441 agggaatgat gaatcgtccg tttttagaaa ctaatagtag taattttatt gaagaattta
     1501 aggtaatatt aaaggagttg tgcgatgaaa atcaataaaa actctttatt ttatattgca
     1561 atatttttag ttaatttttt taaatcactg ggtttaggcg agggaaactc agcttacaaa
     1621 atagtgatgt tagttgcaat tttactgtgt ggaataaaat ttttattaga tagcctttat
     1681 tttgaaagaa gaaaactcgt gatcatcttt ttattattta tcgcgaccat tttgaat
//
LOCUS       DATRNK010000136         1657 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.137, whole genome shotgun sequence.
ACCESSION   DATRNK010000136 DATRNK010000000
VERSION     DATRNK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.137"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..294)
                     /locus_tag="VBC52_002063"
     CDS             complement(<1..294)
                     /locus_tag="VBC52_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883607.1"
                     /translation="MEKQQLNRVEKITKLNSRYYLVSICGRYYILDYANPKNYKDYLV
                     GLFPERLNSYDIFDVTDDKNIFLKKGKNSKVTTKGATIFLFLYLIHITIFPNNI"
     gene            complement(300..1136)
                     /locus_tag="VBC52_002064"
     CDS             complement(300..1136)
                     /locus_tag="VBC52_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883608.1"
                     /translation="MEFNAESSTVFGGLTEVQDAVSAGLNYVSTGFGNFNGTFDTSGK
                     GLDWIKTIKTGMEVRKEARDKELKKKKPNESYDGKDALADFQTETTDYVNYGTEAAGQ
                     QFDKFGKIIIKDGKNIGKVYGAKLQPRDANGRFVKDTNPLRKSATSKLKDITNAESRV
                     IGRAARVGGTALVAYNFYSDAKEHYDRHHNVGRAMSYSGLTTTTGVAAGAIGASVGTG
                     LATSAGFAAPLVGAVVIGAIAVIGAKAIYENVKPVRRAIDSVGDALNNIGKSFSSALK
                     WG"
     gene            complement(1400..>1657)
                     /locus_tag="VBC52_002065"
     CDS             complement(1400..>1657)
                     /locus_tag="VBC52_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HEO6883609.1"
                     /translation="VMIIARYPLYKYKNRIGYFDYSGCIFPSGVTDNQTYFFNNDDIE
                     KVWFTGYIDENELKAQKVFKEQIDKIEYPHFTIEEVNKIEE"
BASE COUNT          534 a          329 c          208 g          586 t
ORIGIN      
        1 aatattattt ggaaagatag taatatgtat taaatataag aataaaaaga tagtagcacc
       61 tttagtagta actttggaat tttttccttt ttttaagaat atattcttat catctgttac
      121 atcaaagata tcataagaat tcaatctctc agggaataaa cctactaaat aatctttgta
      181 gttttttggg ttagcataat ctagaatgta ataacgtcca cagatactta ctaaataata
      241 tctactattt aattttgtaa ttttttcaac acgatttaat tgttgttttt ccaattagtc
      301 tacccccatt ttaatgcaga actgaaagac ttacctatat tgttaagagc gtctccaaca
      361 ctgtcaattg ctcttcgcac aggtttaaca ttttcataaa ttgcttttgc accaatcacc
      421 gcaattgctc ctataacaac tgctcccacc aatggtgctg caaatccagc agatgtagct
      481 aatccagtac ctacactagc tcctattgct ccagcagcca caccggttgt cgttgttaat
      541 ccgctatatg acattgctct tccaacatta tgatgtctat cataatgttc ttttgcatct
      601 gaataaaaat tatatgctac taaagcagta ccaccaacac gtgcagccct ccctataact
      661 ctactctcag catttgtaat atctttaagt ttgcttgttg cacttttacg caaaggatta
      721 gtatccttta cgaaccgtcc gttagcatct cttggttgta attttgcacc ataaacttta
      781 cctatatttt tgccatcttt aataataatt ttcccaaatt tatcaaattg ttgacctgca
      841 gcctcagtac cataattcac ataatctgta gtttctgttt gaaaatctgc aagagcatct
      901 tttccatcat aagattcatt tggctttttc ttcttcagtt ccttgtctct agcttctttt
      961 cgaacttcca tcccagtctt gattgttttt atccaatcaa gacctttacc tgaggtatca
     1021 aatgtaccat tgaaatttcc aaaacctgtc gagacatagt taagtcctgc cgaaacggca
     1081 tcctgaactt ctgttagtcc accaaagact gttgaggatt ccgcgttgaa ttccatgaga
     1141 tgttttttaa aacataggtt acctttaaag ccagaaaact cctaagagat ttcatgaact
     1201 acctgtcgct ttataagtga tcacaagcta ataacaaaag tgaagtgaat cattaacttt
     1261 tctaaagtta ataaagaatg gaattagctc aattgaaact atctacttaa gaacaaagaa
     1321 gtaccttaac tttcatctct gttcttaaat agatagtttt ataaaaacaa ctatctaaaa
     1381 tatattaata acgacaatat cactcttcaa tcttgttaac ttcttcaatt gtaaaatgtg
     1441 gatattcaat tttatcaatt tgttccttaa aaactttttg agcttttaac tcattctcat
     1501 caatataacc tgtaaaccac accttttcaa tatcatcgtt attaaaaaaa tatgtctgat
     1561 tgtcagtaac tccactagga aatatacaac cagaatagtc aaaatagcca attctattct
     1621 tatatttata taaaggataa cgggcaataa tcattac
//
LOCUS       DATRNK010000137         1580 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.138, whole genome shotgun sequence.
ACCESSION   DATRNK010000137 DATRNK010000000
VERSION     DATRNK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.138"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..1429)
                     /locus_tag="VBC52_002066"
     CDS             complement(<1..1429)
                     /locus_tag="VBC52_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6883610.1"
                     /translation="MIENFLEKNILNKVKLLTLCYDTPSLSLDTACNRLHLSPVELKN
                     YCHKLNVLFGHKLSIHIQKERILCKLNKLTQEDALKIVYNESNALQLLKFLIDDSSDK
                     KSLVAFGNDLFLSRSSVYRLRENLIPLIKDLGLSLSKNTIVGDEYRIRYLIAYLTTKF
                     GIKIYDLSALDIKIIRQFLFESGTNIRPTTHLSDIFIFFDTLFILTWKRHHHKVKIPN
                     SKLFSSLKKIFIYEELNTCVKSIVQPYFNVIFNSEDMDYIFLIYLTSANSFAAQKWTE
                     THVDEVLAIFKSLPKFQMLLQPLKEALPYSDTYYDELVKVTIYFSRNFIVGLNQLIPE
                     TISFPSTFYPGNPKLIPIIEPIVTHWLTEIGESTLKKQHFFLFCAHLERVIKNNMPPI
                     RVVLLTTDFINNQIFKDYLLQSLSLEKINFHSYYLLRDNVSQVSTLKPDLIITDQKLV
                     PYVTKELATNSLVAHIDYNDTPSQIS"
BASE COUNT          576 a          190 c          273 g          541 t
ORIGIN      
        1 tactgatttg agagggtgta tcgttgtaat caatatgagc gactaaacta tttgtagcta
       61 attcctttgt gacatatgga actagttttt gatcggtaat gattaaatca ggttttagag
      121 ttgatacttg actaacatta tctcttaata agtaatagga atgaaagttt attttctcta
      181 aggaaagaga ttgtaataga tagtctttga agatttgatt attaatgaaa tcagttgtta
      241 acaacacaac tcttatagga ggcatattat tttttatcac cctttccaga tgagcacaga
      301 ataagaaaaa gtgctgtttt tttaaagttg attccccaat ttcagttagc caatgtgtga
      361 ctattggttc gataatgggt attagtttag gatttccagg atagaatgtt gaaggaaaac
      421 taattgtttc aggaattaat tgattcagac caactatgaa gtttcgagaa aagtaaatag
      481 tgactttcac tagctcatcg tagtaggtgt cagagtatgg aagagcttct ttcaaaggtt
      541 gtagtagcat ttggaattta ggaagacttt taaaaatagc taacacttca tcgacatgtg
      601 tttcagtcca cttttgagca gcaaaagagt tagcgcttgt aagatagata agaaaaatat
      661 aatccatgtc ttctgaatta aaaataacat tgaagtaagg ttgaacgata ctttttacac
      721 atgtattcag ctcctcataa ataaagattt ttttaagaga agagaaaagt ttggagtttg
      781 gaattttaac tttatgatgg tgacgtttcc aggttaaaat gaaaagcgta tcaaaaaaaa
      841 taaagatatc agaaaggtgc gtagtaggtc ttatattagt accactctca aataaaaatt
      901 gtcgaataat cttaatatct agagcagaca gatcgtaaat tttaatacca aatttagtag
      961 tgaggtatgc gatgagatat ctaatgcgat attcgtcacc tacaatggta tttttagata
     1021 aagataatcc taaatcttta attaaaggaa ttaaattttc tcgcagacga tagacagaag
     1081 aacgagataa gaagagatca tttccaaaag ctactagaga ttttttatca gacgagtcat
     1141 caattaaaaa tttaagaagt tgtaaagcgt ttgactcatt ataaactatt tttaaggcat
     1201 cttcctgagt aagtttattt agcttacata aaatccgttc tttttggata tggatagaaa
     1261 gtttatgacc aaataaaaca tttaatttat gacaatagtt tttaagctca actggtgata
     1321 agtggagtcg gttacaggcg gtatctaagg atagtgaggg ggtatcataa cataaagtaa
     1381 gtaatttaac tttattaaga atattttttt ctaaaaaatt ttctatcata ttacacaact
     1441 cctttcactc attaatacta taataaggaa taatataaaa tgtttcaaat attttaacct
     1501 tataaaataa taattctcaa aaaacaaata ttttaaatac ctttttggga aaatgagtta
     1561 taaaagtata tttattttag
//
LOCUS       DATRNK010000138         1529 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.139, whole genome shotgun sequence.
ACCESSION   DATRNK010000138 DATRNK010000000
VERSION     DATRNK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.139"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(220..408)
                     /locus_tag="VBC52_002067"
     CDS             complement(220..408)
                     /locus_tag="VBC52_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, toxin component, MazF
                     family protein"
                     /protein_id="HEO6883611.1"
                     /translation="MSDIEVYSVLVSRIEYSDGTGSKVRPAVVVKFNDEVIKTLRFTT
                     KYENKSDNIRSQYLEVID"
     gene            complement(405..698)
                     /locus_tag="VBC52_002068"
     CDS             complement(405..698)
                     /locus_tag="VBC52_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEO6883612.1"
                     /translation="MSTVAVRVDDQLKDDATELFQSLGLDMSTAVKMFLIQSVKTQSI
                     PFEIKNQSSVSDEEFQNLVETKLKGIRVKASDPESVNAFFGDEDFSEYEEYFK"
     gene            755..904
                     /locus_tag="VBC52_002069"
                     /pseudo
     CDS             755..904
                     /locus_tag="VBC52_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(1410..>1529)
                     /locus_tag="VBC52_002070"
     CDS             complement(1410..>1529)
                     /locus_tag="VBC52_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001920755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6883613.1"
                     /translation="GKGNKLPATGENATPFFNVAALTIISSVGLLSVSKKKED"
BASE COUNT          545 a          284 c          177 g          523 t
ORIGIN      
        1 tcgaacaagc aactaaaaat actgtagata tagctaaaat catcaatcct aataatttct
       61 ttttcataac taaacttcct ttgctatgag aaaatctttt aacctctcaa catctctatc
      121 gcttaactta cctataattt taatattgaa ccctttattc tcaacatcat aatattgaat
      181 agtatctatc caagaacgcc tatttaaatt tgcttttgcc taatcaataa cttctaaata
      241 ctgacttctt atattgtcag acttattttc atatttagta gtaaatctta aagtttttat
      301 tacttcatca ttaaatttga ctactacagc aggacgaacc tttgaacctg taccatcaga
      361 atattcaata cgtgaaacta atacagaata tacctcaata tcactcattt aaaatattcc
      421 tcatattctg aaaaatcttc atctccaaaa aacgcattta cactttctgg atcactagcc
      481 ttaactcgta ttccttttaa ttttgtttct accaaatttt gaaattcttc atcagaaaca
      541 gatgattgat tttttatttc aaaaggaata ctttgagttt taacagactg aatcaaaaac
      601 atttttactg ctgtactcat atccaaccct aaggattgaa aaagttctgt agcatcatct
      661 tttaattgat catctactct tactgctact gttgacataa tatcacctca aatcattttt
      721 gtataaccat tgtatcacta actgcaatac aacgcaaaca aaaaagaaaa tcgtatttcc
      781 cagtgaagag accctagaac attgtctagt cacactattt aatgattaca acttcaagca
      841 agaacaacga gtccacaaag gttttggtca ttgtacagac acacttgaaa gcctatttga
      901 ttaacaatcc cagctttgcc agtgtgttta cacaacatta ttgacacact ccttttattt
      961 ttataaattc cccttaaata catttaatta cttgaaaaga caaacagtca ttttagaaaa
     1021 ttttgcatct aaccttacat ttgattacac ctaaaaattt taaatcaaca aaagagagcc
     1081 taggattaac atcttaggct ctaatgatac acttcattta caaatttcaa ctaaatagga
     1141 tcatttcttc agttttatag taagcgtcca ataacaaggt atattggatg cttacggaat
     1201 agggaagaaa atggaagata cctcgttatt ggacgcttac acaaaatcaa aagcctatcg
     1261 aaattaataa tctcgatagg ctttttcaag aattaaactc gaaaaattga aacacattaa
     1321 ttaagtttgt tggagagaga tacataactt tattaatgtg ttcacaccaa taaatggtga
     1381 aatgtttagt gacattttag gtcaaaagat taatcctctt ttttcttaga aacagataat
     1441 aaaccaactg atgatataat tgtcaaagct gcaacattaa agaatggagt tgcattctca
     1501 cctgttgctg gtagtttatt tcctttacc
//
LOCUS       DATRNK010000139         1472 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.140, whole genome shotgun sequence.
ACCESSION   DATRNK010000139 DATRNK010000000
VERSION     DATRNK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.140"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            161..1444
                     /locus_tag="VBC52_002071"
     CDS             161..1444
                     /locus_tag="VBC52_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO6883614.1"
                     /translation="MSLYYTEKAHAIAGPSDRQYVENPNPHIIVNVTGTDQNGNSILP
                     HYIEVNVNMGQTLSKKEILDYIARNLNSSVGGESKNVQYSNIEFKESAYLKRQLDDGK
                     TEEIAIDNDGVTVPKDGPNKFWIDVPVTCTVTPIVTETHEVRWGTPVDISHRIYFVEE
                     SSGKVLDEYTNLHTAYSEFNGYRVGDYITDYALSKSAYEAFLNSRLDKEGYKLQHRIS
                     TNVRQNLQIDKLIFNYDFNEENIHYQIGNIRPLLSRSSAEVESDIITERYYVSKNAKS
                     LARTESTISIKMVDAKTEQALFNHTLTGYQLSTVSNVYNRLFEENLIPTTKSGEKYFI
                     QNMKKTAEQEYTVYLSETPYSEENAPVISYDARPVDWDYHSGASGSLENQPNIYTEED
                     STEFLGNKPQAACYPNKQFACENTDSKYNYSYLEK"
BASE COUNT          527 a          265 c          241 g          439 t
ORIGIN      
        1 tctcagtata acttagtgtc gtagagatta attcctatca ttcaccgctt tattttagga
       61 taactaaaca atttattgtt taaaacgaaa ggatttatga atgtctagga aaacatttaa
      121 acgtatacta tcgattggag tttgcacgct cgtactatcg atgagtcttt attacactga
      181 aaaagcccac gctattgctg gtcctagtga ccgccaatac gtagaaaacc caaatcctca
      241 cattattgta aatgttacag gtactgatca aaacggaaat agcattttac cgcattatat
      301 cgaagtcaat gtaaatatgg gacaaacttt aagtaaaaaa gaaattctag attatattgc
      361 tcgaaattta aactctagtg ttggaggaga aagtaaaaac gttcaataca gcaacatcga
      421 gtttaaggaa agtgcttatc tgaagcgtca attagatgat ggcaagacag aagaaatagc
      481 aattgataac gacggtgtta ctgtacctaa agacggtcca aacaaatttt ggattgacgt
      541 tccagtaact tgtactgtta ctcctatcgt aacagaaaca catgaagttc gatgggggac
      601 tccagtcgat atatcacacc gtatttactt tgttgaagaa tcttctggaa aagttttaga
      661 tgaatacaca aatctacaca ctgcttattc ggaatttaac ggttatcgtg ttggagatta
      721 tatcacagac tatgcacttt ctaagtctgc ttacgaagct tttttaaact ctcgtttaga
      781 taaagaaggt tacaaacttc aacatcgtat tagcacgaat gtacgacaaa accttcaaat
      841 tgataaattg attttcaatt atgactttaa tgaggaaaat attcactacc aaatcgggaa
      901 tatccgtcca ctattaagtc gctcatcagc tgaagtagaa tctgacatca ttacagaacg
      961 ctactatgtt tctaaaaatg ctaaaagttt agcacgtaca gaatcaacca tttcgattaa
     1021 aatggttgat gccaaaactg aacaagcgct atttaaccac acattaacgg gttatcaatt
     1081 gtcaactgtt tccaatgtct ataacagact ctttgaagaa aatcttatcc caactacaaa
     1141 atcaggagaa aaatatttta ttcaaaatat gaaaaaaaca gctgaacaag aatatactgt
     1201 ttacctttca gaaacacctt attctgaaga gaacgctccc gtaatttctt atgatgcaag
     1261 acctgttgat tgggattatc actcaggcgc ttcaggatca cttgaaaatc agcctaacat
     1321 ctatactgaa gaagattcaa ctgaattttt gggtaataaa ccacaagcag cttgttatcc
     1381 aaacaaacaa tttgcttgcg aaaatactga ctctaaatac aactatagct atttagaaaa
     1441 atagttataa ccgatagggt taatctctca tt
//
LOCUS       DATRNK010000140         1467 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.141, whole genome shotgun sequence.
ACCESSION   DATRNK010000140 DATRNK010000000
VERSION     DATRNK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.141"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..196
                     /locus_tag="VBC52_002072"
     CDS             <1..196
                     /locus_tag="VBC52_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6883615.1"
                     /translation="SSKRAEDLIYIFDSKLSEIILSGLDSTATVERYFWLMRDYKQRL
                     EQENLQKTIIYKVISNRLSR"
     gene            257..1453
                     /locus_tag="VBC52_002073"
     CDS             257..1453
                     /locus_tag="VBC52_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEO6883616.1"
                     /translation="MRVIKVKTISETLDYISKHTASVARFGDGEIDIIAGNSIPYQVY
                     DSELAKRLKRLLNRQSNEKLVICVSDVFDNLGRYNDFSQQFWRGHLYQYNDLYQKNCQ
                     AEWYGSTFISRPYMDLLDKSLSVTYFEQLKALWKNKDILIVEGETSRSGVGNDLFNDA
                     NTISRIICPSKDAFSSFYEIKETILEHSRGKLILLMLGPTAKLLAEDLSQLGHQAIDL
                     GHIDSEYEWFKMGAVSKVKLSHKHTAEHNFDEDITLIEDNSYENQIVSKIGTSDRQYA
                     LTVTLTDDIWELEHLLQRCPNTDFHIAAPVYCSDRLKQLVGYPNYYLHEAITEEQFEV
                     LLLNSDIYLDINHGEEVWNVVDRCITEGKTIYGIRRARKDNQYISFERTMDDFEYLCD
                     TIKQNR"
BASE COUNT          516 a          188 c          280 g          483 t
ORIGIN      
        1 ttcatccaaa agagcagaag atcttattta tatttttgat agtaaattgt cggagattat
       61 actgtcagga ttggattcaa ctgcaactgt agagagatat ttttggttaa tgcgtgacta
      121 taaacagcgc ttagaacaag aaaatttaca gaaaacaata atatataaag tgatatcaaa
      181 tcggttatca aggtaacagt aaataccatt ttatttgtca taatcttgca aagagagttg
      241 aaaagaacta atagttatga gagttattaa agtaaaaaca atttcggaaa ctttagatta
      301 tatttctaaa catactgctt cggtagcgcg ttttggtgat ggagaaattg atattattgc
      361 ggggaattct attccttacc aggtttatga ttctgaactt gctaagcgat taaaacgttt
      421 attaaatcga caaagtaatg agaagctagt tatctgtgta agtgatgttt ttgataattt
      481 aggtagatac aacgattttt cacaacaatt ttggaggggg catctatatc agtataatga
      541 tttgtaccaa aaaaattgtc aggctgaatg gtatggttca acttttattt ctagacccta
      601 tatggattta ctagataaga gtttgtcagt aacgtacttt gaacagctaa aagcattatg
      661 gaaaaataaa gatattttaa ttgtcgaggg tgagacttct cgttcaggcg ttggcaatga
      721 cttatttaat gatgccaata ctatttcgcg tatcatttgt ccttcgaaag atgcattttc
      781 atctttttat gaaatcaagg aaactatact tgaacatagt agaggtaagc taattttact
      841 tatgcttggt ccaactgcaa agctattagc tgaagattta tcgcagctag gacatcaagc
      901 tattgattta ggtcatatcg attcagaata tgagtggttt aagatgggag cagtttctaa
      961 ggtgaaactt tctcataaac atacggcaga acataatttt gacgaagata ttactttaat
     1021 agaagacaat agttacgaaa atcaaattgt atcaaaaatt ggtacatcag atagacagta
     1081 cgctttgaca gtgacattaa cagatgatat atgggagcta gaacatttat tacaaagatg
     1141 tcctaatact gattttcata ttgctgcccc agtttattgt tcggatagac ttaagcagtt
     1201 agtaggttat ccaaattatt acctacatga agctattact gaagagcaat tcgaagtact
     1261 cctattaaat agcgatatat atttagatat taatcatggt gaagaagttt ggaatgttgt
     1321 tgatagatgt ataactgagg gtaaaactat atatggaatc agacgggcaa gaaaagataa
     1381 tcaatatatt tcttttgagc gaaccatgga tgattttgaa tatttgtgtg acactattaa
     1441 acaaaataga tagtcaagaa gagtaat
//
LOCUS       DATRNK010000141         1435 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.142, whole genome shotgun sequence.
ACCESSION   DATRNK010000141 DATRNK010000000
VERSION     DATRNK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.142"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            180..359
                     /locus_tag="VBC52_002074"
     CDS             180..359
                     /locus_tag="VBC52_002074"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883617.1"
                     /translation="MRIVEASSGTPQGGEQLLLILGYANGSELTNFTLNFTLGDNQMP
                     NIDSVGSDNLSTIED"
     gene            429..599
                     /locus_tag="VBC52_002075"
     CDS             429..599
                     /locus_tag="VBC52_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883618.1"
                     /translation="MAESANTGRSNSFVLEELTEFQREKLKNGESVKFNFSKDVKKEN
                     KVIEGGHPQVIG"
BASE COUNT          510 a          172 c          251 g          502 t
ORIGIN      
        1 atgaatagtg aatctaaaag tcagtcaaac gaagtaaaaa atagcaaaca gtcagcagta
       61 aagaaaggta aaaaagtaat aaaaaaagaa caagtagctt atctcaaaaa gcatgaaaaa
      121 gaattaactg aatatgtcaa aatccagata ttagtgaaat taagtatgat tggaatagtg
      181 tgcgcatagt ggaagctagt agtggaaccc ctcaaggcgg agagcaacta ttattaattt
      241 tgggttatgc aaatggcagt gaattaacca attttactct aaatttcaca ttgggtgata
      301 atcaaatgcc taatattgac tcagttggct cagataattt atcaactatt gaagattaat
      361 aataacaata agaaaggtat ataatgggat gatggaatct taaggaattt gaagatatat
      421 tttgaagtat ggcagaaagt gctaatacag gtcgatcgaa ttcttttgtt ttagaagaat
      481 taactgaatt tcaaagagaa aagttaaaaa atggggagtc agttaagttt aatttttcta
      541 aagatgttaa gaaagaaaat aaggttatcg aaggagggca cccccaagtc atagggtaat
      601 ctacctgcaa actggataag tccttaaata caattgatga aaaagtagag gttcgcgttc
      661 ctgatgtcaa tggcggttat cactctgagc attgtgttac taattcctat cagaaaggtt
      721 actgaaagat gatgaagcag taactctata atcactaaaa gctcatcatt gcttagtgtt
      781 taagttaact tctaaagctc aaaccttgtt aaatcaaggt ctgtttgctt ttaaaagtga
      841 catgttattt ttctaaaaat gagattattg ggattcctca ttctaagtta ctttgtttat
      901 atgtaccttt acttaaggtt tattttcatt atttttaatt taatattttg gagtatgtat
      961 gctccattat agtattttcg aagacatctt taaaaggact gtagaagtta aaatatttta
     1021 acctagtatc taacgtcata tattggtaca tagttttaag tatccttttg ataaccttgt
     1081 acatatgaaa taggctagat atttttagtg aactaaatgt aagtttattg cttgtattag
     1141 gcgggataac aatattgttg tttttattct ttctgagtaa tggtttatta atcatgtaat
     1201 tattctggta tcaaatgata cttttaatat tccagtttgt caataaatca tcaatgtaat
     1261 tcttttcatc aaaagcgatc cctttatcta gttgatttaa tatgttaact tgatgattgt
     1321 tcttcttgct taatatatgg gataataatt atcttaaatt gacacttgga gcattcaatc
     1381 acgtgatgta ataactaaaa attgtttaaa atttaaggat gtatttcttg ttgat
//
LOCUS       DATRNK010000142         1426 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.143, whole genome shotgun sequence.
ACCESSION   DATRNK010000142 DATRNK010000000
VERSION     DATRNK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.143"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            79..1347
                     /locus_tag="VBC52_002076"
     CDS             79..1347
                     /locus_tag="VBC52_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEO6883619.1"
                     /translation="MKIDKRHLLNYSILIPYLILSILGLIVIYSTTSATLIQLGANPF
                     RSVINQGVFWAVSLVAIIFIYKLKLNFLKNSKVLTMAVLVEVFLLLIARFFTQEVNGA
                     HGWIVIGPISFQPAEYLKVIIVWYLAFTFARRQKKIEIYDYQALTKGRWLPRSLSDLK
                     DWRFYSLFMIGLVIAQPDLGNGSIIVLTVIIMYCISGIGYRWFSALLGLIVVGSTLFI
                     GTIAVVGVETMAKVPVFGYVAKRFNAFFDPFKDLTDSGHQLANSYYAMSNGGWFGRGL
                     GNSIEKLGYLPEATTDFVFSIVIEELGVIGAGFILALVFFLILRIMHVGIKAKDPFNS
                     MIALGIGAMLLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAIGFVLNIDAN
                     EKKELIMKEAEEQYKPQEKNEKIINLDAFK"
BASE COUNT          428 a          208 c          275 g          515 t
ORIGIN      
        1 tctccctttt actttacttc gaaagatgat ataatgcata atggtaattt tttgatatat
       61 taaaggaaat aatacaatat gaaaattgat aaaaggcatc ttctgaatta ttccatcctc
      121 attccctatt tgatattgtc tattctaggc ttaattgtaa tttattcgac aactagtgca
      181 actctaattc agcttggagc taaccctttt cgttcagtaa tcaaccaggg tgtcttttgg
      241 gcagtcagtt tggttgcaat tatctttatc tataaactga aactaaattt tttgaaaaat
      301 tctaaagtgt tgactatggc agtattggta gaagtatttt tactactaat agcgcgcttt
      361 tttacacaag aagttaatgg agcacatggt tggattgtta ttggtcctat tagttttcaa
      421 cctgctgaat atttaaaagt tatcattgta tggtatttag cgtttacttt tgctcgaagg
      481 caaaaaaaga tagaaatata tgattatcaa gctttgacaa aagggagatg gcttccgaga
      541 tcactaagcg acctaaaaga ttggcgattt tattctttat ttatgattgg tttggtaatt
      601 gcacaacctg atttaggaaa tggttcgata attgttctaa cggtcattat tatgtactgt
      661 attagtggta ttggttatcg atggttttca gctttacttg gtctaatcgt cgtggggtca
      721 actttattta tcggaactat tgcagttgtt ggagttgaga caatggctaa agtccctgtc
      781 tttggttatg tagctaagcg atttaatgct ttttttgacc catttaaaga tttaacagat
      841 tcaggacacc agttggctaa ttcgtattat gcaatgagca acggtggctg gtttggtcga
      901 ggacttggga attctattga aaaattaggg tacttaccag aagcaacaac agactttgtc
      961 ttttcaattg ttattgagga attaggagtt attggtgcag ggtttatttt agctttggtt
     1021 ttcttcttaa ttttacgtat tatgcacgta ggtataaagg ctaaagatcc tttcaactca
     1081 atgatcgctc ttggtattgg agcaatgcta ttgatgcaag tttttgttaa cattggtggt
     1141 atttctgggt taatcccctc aactggagtg acatttcctt tcttatccca aggagggaac
     1201 agcctcttgg tactttctgt agctatcgga ttcgtgctta atattgatgc taacgagaaa
     1261 aaggaactca taatgaaaga agcagaagag caatataagc cacaagaaaa aaatgaaaaa
     1321 ataatcaacc ttgatgcctt taaataatca tccaaacaaa aatcgagatt tttatctcga
     1381 tttttgtatt ttttgaaatt taaactctaa ttaatagaga gaagat
//
LOCUS       DATRNK010000143         1410 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.144, whole genome shotgun sequence.
ACCESSION   DATRNK010000143 DATRNK010000000
VERSION     DATRNK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.144"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..681
                     /gene="recX"
                     /locus_tag="VBC52_002077"
     CDS             <1..681
                     /gene="recX"
                     /locus_tag="VBC52_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEO6883620.1"
                     /translation="FMLSKGMIINAEKLENIKKFAQLSYGKNLGLYYISFKQRTEKEV
                     IKYLQQHDIDSKIIPQIIDNLKSENWINDKNYVQSFIQQNLNTGDKGPYVIKQKLLQK
                     GIKSKIIESELQAINFQDLASKISQKLYKKYQNKLPLKALKDKLMQSLTTKGFDYQIV
                     HTVIQNLEIEKDQELEEDLIYKELDKQYQKLSKKHDQYELKQRIINALMRKGYQYEDI
                     KSALREYL"
     gene            765..1298
                     /locus_tag="VBC52_002078"
     CDS             765..1298
                     /locus_tag="VBC52_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEO6883621.1"
                     /translation="MRLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTENDGRRWVTREPAIVYFHKKYWFNIIAMIRETGVSYYCNLASPYILDPEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEQHKAQMNYPTDIDYILKENVKILVEWINENKGPFS
                     SSYINIWYKRYLELKKR"
BASE COUNT          589 a          207 c          214 g          400 t
ORIGIN      
        1 tttatgttaa gtaaagggat gataattaat gccgagaaac ttgaaaatat taaaaaattt
       61 gcccaactat catacggtaa aaatctaggt ctttattata tttctttcaa gcagagaacc
      121 gagaaagaag ttattaaata tcttcaacaa catgatattg attctaaaat tattccacaa
      181 attattgata atctaaaaag tgagaattgg ataaacgata aaaactacgt gcaatctttc
      241 atacagcaaa atctaaatac tggcgataag ggaccatacg tgataaaaca aaaattattg
      301 caaaaaggta tcaaaagtaa aataattgaa tctgaacttc aagctataaa ctttcaagac
      361 ttagcatcca aaataagcca aaaactctat aaaaaatatc aaaacaaact tccactaaaa
      421 gcattaaaag ataaattgat gcagagctta actacaaaag gatttgatta tcagatagtg
      481 cataccgtaa ttcaaaacct tgaaattgaa aaagatcaag aacttgaaga agacctcatt
      541 tataaagaat tagataaaca ataccaaaaa ttatcaaaaa aacatgacca gtatgaatta
      601 aagcaacgta taatcaatgc cttaatgcgt aaaggatacc agtacgaaga tatcaaaagt
      661 gctttaagag aatatttata agataataac tctcagacga tgtattttac agaaaattat
      721 gataaactat aacagacgta taaattgtag aaagttggta ggctatgaga ttacctaaag
      781 aaggcgactt tattacaatt caaagttaca aacatgatgg tagtttacac cgaacttggc
      841 gtgacaccat ggtattaaaa acaaccgaaa atgccctcat tggtgttaat gatcatactt
      901 tagtaacaga aaatgatggt cgacgctggg tgacacgaga gcctgcaata gtatactttc
      961 ataaaaaata ctggtttaac attatcgcta tgatacgtga aactggtgtc tcctactatt
     1021 gtaatctagc aagtccgtat atcttggacc ctgaagcact caagtatatt gactatgacc
     1081 ttgatgtcaa agtatttgca gatggtgaaa aaagactact agatgtggac gaatatgaac
     1141 agcataaagc tcagatgaac tatcctaccg atattgatta tatattaaag gaaaatgtaa
     1201 aaatattggt agaatggata aatgagaata aaggcccctt ttcatcatca tatatcaata
     1261 tctggtataa acggtacctt gaattgaaaa agcgctaact aacacactaa atagtgtgtt
     1321 tttattaata tcaaatttaa ttacaatact attgcaaaaa tatatactta aaataaaaaa
     1381 agtagaaaga tcactttcta ctttttttaa
//
LOCUS       DATRNK010000144         1396 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.145, whole genome shotgun sequence.
ACCESSION   DATRNK010000144 DATRNK010000000
VERSION     DATRNK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.145"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            246..638
                     /locus_tag="VBC52_002079"
     CDS             246..638
                     /locus_tag="VBC52_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEO6883622.1"
                     /translation="MQSQKLNVLQRVLRDKFLLQGLGVPNSNTVLNRSFTALLLALLL
                     EDSNNKSWISEEDQIKIMNQACSWLIDEPDFRGFDEELGWIHAFAHGADLLTEIVKMK
                     YFSKDDSLKILTIIKRVMITDDILTLNG"
     gene            817..>1396
                     /locus_tag="VBC52_002080"
     CDS             817..>1396
                     /locus_tag="VBC52_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HEO6883623.1"
                     /translation="MRDEQEIYNLVLNIANQDKRIEAVLLNGSRANPNVPKDDFQDYD
                     IVFVTNFIEDIISDTNYHKKFGDILIMQKPNEFRNKTEYNCFAYLMQFQDLTRIDLRL
                     IKPEFLEDYLDDAFSKVLLDKKNKYLDYNFERSSLYETKQLSEDEINKILNEIYWVST
                     YVVKGIARNDIIYSEFMISNPIKNAFIKLLKQK"
BASE COUNT          547 a          154 c          251 g          444 t
ORIGIN      
        1 gttactataa tcattttaaa gtgaaaacga aaactctaat gttacacttt ggaaaatata
       61 aaatataaag tcatgcaagg agaaacatgg aagcaagtga tgtaatcaaa aagatagaaa
      121 ataattatcc aattgatgag gatgttttat ctttttgatt acatcatata ggagattgga
      181 acccaagagt tcgagataaa atagtatatg ggtcttgggt taaattagta atagaacata
      241 aactgatgca aagccagaaa ttaaatgtcc ttcagagagt tttaagggat aaatttcttt
      301 tacaaggatt aggagtaccc aacagtaata ctgtgttaaa cagatctttt actgctctat
      361 tactagcttt attattggag gactcaaata ataaaagttg gatatctgaa gaagatcaga
      421 taaaaattat gaatcaggca tgctcctggc ttatagatga acctgatttt agaggctttg
      481 acgaggagct tggatggata catgcctttg ctcatggagc tgatttatta acagaaatag
      541 tcaagatgaa atatttttca aaagatgata gtttaaaaat cttaactatc ataaaaagag
      601 tgatgataac tgatgacata ctaacattaa acggttgaaa atatttggaa tcataatcag
      661 ttgttcagaa aaatttaaaa ttgaatcctt ggaggtcgta aaggtatgtc catgctaatt
      721 taagtttatg attacatgct tagagaaaaa tacaagggta atataaaggt attagaaaaa
      781 acatggtaat ataaagtgaa gtaggaggta gccattatga gagatgagca agaaatatat
      841 aatttggttt taaacatagc aaaccaagat aagagaatcg aagctgtact attgaatgga
      901 tctcgggcta acccaaatgt accgaaagat gattttcaag attatgatat tgtttttgtt
      961 actaatttta tagaagatat tatttctgac actaattatc ataaaaaatt cggtgatatt
     1021 cttataatgc agaaaccaaa tgagtttaga aataagacgg aatacaattg ctttgcttac
     1081 ttaatgcagt ttcaagatct gacgaggatt gatttaaggc ttattaaacc agaattcttg
     1141 gaggattatt tagatgatgc tttttcaaaa gtacttttag ataaaaagaa taaatatctt
     1201 gattataatt ttgaaagaag ttctctgtat gaaacaaagc aattgtcaga agatgaaatc
     1261 aacaaaattc ttaatgaaat atattgggtt agtacttatg tagtcaaggg gatagcaaga
     1321 aatgatatta tttattcaga gtttatgata tctaatccta ttaaaaatgc ttttattaaa
     1381 cttctaaaac aaaaaa
//
LOCUS       DATRNK010000145         1394 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.146, whole genome shotgun sequence.
ACCESSION   DATRNK010000145 DATRNK010000000
VERSION     DATRNK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.146"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            109..>1394
                     /gene="alp3"
                     /locus_tag="VBC52_002081"
     CDS             109..>1394
                     /gene="alp3"
                     /locus_tag="VBC52_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_115075552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-like surface protein Alp3"
                     /protein_id="HEO6883624.1"
                     /translation="MFRRSKNNSYDTSQTKQRFSIKKFKFGAASVLIGLSFLSGVTQG
                     NLNIFEESIVAASTIPGSAATLNTSITKNIQNGNAYIDLYDVKNGLIDPQNLIVLNPS
                     SYSANYYIKQGAKYYSNPSEITTTGSATITFNILDETGNPHKKADGQIDIVSVNLTIY
                     DSTALRNRIDEVINNANDPKWSDGSRDEVLTGLEKIKKDIDNNPKTQIDIDNKINEVN
                     EIEKLLVVSLPDKIKYSPEAKHRTVEQHAELDAKDSIANTDELPSNSTYNWKNGHKPD
                     TSTSGEKDGIVEVHYPDGTVDDVNVKVTVTSKKTDNTAPTLTVTPEQQTVKVDEDITF
                     TVTVEDENEVELGLDDLKAKYENDIIGARVKIKYLTKESNKKVMEVTIMKATLADKGA
                     ITFTAKDKAGNQAEPKTVTINVLPLKDSNEPKGKDQT"
BASE COUNT          559 a          194 c          253 g          388 t
ORIGIN      
        1 aaaaaaaaag cagattagat tagataaaaa agtatagata ttctaatatt tattgtttaa
       61 gcctataatt tactctgtat agagttatac agagtaaagg agaatattat gtttagaagg
      121 tctaaaaata acagttatga tacttcacag acgaaacaac ggttttcaat taagaagttc
      181 aagtttggtg cagcttctgt actaattggt cttagttttt tgagtggggt tacacaaggt
      241 aatcttaata tttttgaaga gtcaatagtt gctgcatcta caattccagg gagtgcagcg
      301 accttaaata caagcatcac taaaaatata caaaacggaa atgcttacat agatttatat
      361 gatgtaaaga atggattgat cgatcctcaa aacctcattg tattaaatcc atcaagctat
      421 tcagcaaatt attatatcaa acaaggtgct aaatattata gtaatccgag tgaaattaca
      481 acaactggtt cagcaactat tacttttaat atacttgatg aaactggaaa tccacataaa
      541 aaagctgatg gacaaattga tatagttagt gtgaatttaa ctatatatga ttctacagct
      601 ttaagaaata ggatagatga agtaataaat aatgcaaatg atcctaagtg gagtgatggg
      661 agtcgtgatg aagtcttaac tggattagaa aaaataaaaa aagatattga taataatcca
      721 aaaacacaaa tagatattga taataaaatt aatgaagtca atgaaataga gaaattgtta
      781 gttgtatcgc taccagataa aattaagtat tcgccagagg ctaagcatag gactgttgaa
      841 caacacgcgg aattagatgc aaaagatagc attgcaaata cagatgaatt gccatcaaat
      901 tcaacgtata actggaaaaa tggtcataaa ccagacacct caacatcagg tgaaaaagac
      961 ggaattgttg aagttcacta tccagatggt actgttgatg atgtgaatgt taaagtaacc
     1021 gttacatcga aaaaaactga taatacagct ccaacattaa ccgtcactcc agagcaacag
     1081 actgttaaag tggatgaaga tattaccttt acggttacag ttgaagacga aaatgaagtt
     1141 gaactaggtt tagatgatct taaagctaag tatgaaaatg atatcattgg agctcgtgtt
     1201 aaaattaagt atcttactaa agaatctaat aagaaagtca tggaagtgac aattatgaaa
     1261 gctactttag cagataaggg cgcaattacc tttactgcaa aagataaagc aggtaatcaa
     1321 gcagaaccta agacagttac catcaatgtt cttccgctta aggatagcaa cgaaccaaaa
     1381 ggtaaggacc aaac
//
LOCUS       DATRNK010000146         1378 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.147, whole genome shotgun sequence.
ACCESSION   DATRNK010000146 DATRNK010000000
VERSION     DATRNK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.147"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(124..>1378)
                     /locus_tag="VBC52_002082"
     CDS             complement(124..>1378)
                     /locus_tag="VBC52_002082"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEO6883625.1"
                     /translation="VVSAPGKRYEEDLKMTDALIQYYQNYINGKDIVKDQTWIINRYQ
                     EIISDLSLGSTIAEEITRSIEQLASLPIENNQFLYDCFLAAGEDNNAKLVATFFNQND
                     IPARYVHPNEAGIIVTKEPCNARIIPGSYDKIENLCLYNEVLVIPGFFGVTEDNQICT
                     FSRGGSDITGSLIAAGIKADLYENFTDVDGIFAAHPGVVKNPHAIPELTYKEMRELAY
                     AGFSVLHDEALLPAYRGRIPLVIKNTNNPQQPGTKIVLKHTRSNIAVTGIASDSRFAS
                     INVSKYLMNREVGFGRKVLQILEDLNISFEHMPTGIDDLSIVLREKELTPIKEQEILN
                     YLTRKLEVDYVDIQHNLSTIVIVGENMKSQIGVTATATQALSREKINITMISQGSSEV
                     SIMFVINSKDEKRAIKALYETFFQK"
BASE COUNT          411 a          238 c          240 g          489 t
ORIGIN      
        1 tgataccaag tggttacgat taaaagtgga aaaactatcc tatgttctaa ataaaatcgt
       61 cctaagtaag cttacttagg acgatttgtt atctatctaa tagtgtaagt agtgtaatag
      121 gtactatttt tggaagaatg tttcatatag tgctttaata gctctttttt catccttact
      181 gtttataacg aacataatgg agacttcgct tgaaccttgt gatatcatgg tgatattgat
      241 tttttctctt gataaggctt gtgtcgctgt tgcagtgact ccaatctgac ttttcatatt
      301 ttcacctaca attacgattg tagatagatt gtgttggatg tcaacgtaat ctacttctag
      361 tttacgagtt aggtaattta agatttcttg ttctttgatt ggtgtcaatt ctttttcacg
      421 taagacaatg gatagatcat ctatgccagt tggcatatgt tcaaaactaa tatttaaatc
      481 ctctaaaatt tgtagtactt ttcggccgaa acctacttct ctattcatta agtatttaga
      541 tacgtttatg ctagcaaaac ggctatcaga agcgatccca gttactgcta tgttactacg
      601 agtatgcttt aaaactattt ttgtaccagg ctgttgggga ttatttgtat ttttaataac
      661 aagagggatt ctgccacgat aggcaggaag taaagcttca tcatgtaaaa ccgaaaaacc
      721 cgcataggct aattcacgca tttctttata agtaagctca gggatagcgt gagggttctt
      781 aactacacct ggatgtgctg caaatatacc atcaacatct gtgaagttct cataaaggtc
      841 tgcttttatg cctgctgcaa ttagggatcc agtaatgtca gatccccctc ttgaaaaggt
      901 acaaatttgg ttatcttctg tgactccaaa aaatccaggg ataacaagaa cctcattgta
      961 tagacataag ttctcaatct tatcataact tcctggaata attcgtgcat tacatggttc
     1021 tttagttaca ataattcctg cttcgtttgg atgaacatat cttgcaggaa tgtcattttg
     1081 attaaaaaac gttgcaacta actttgcgtt gttatcttca ccggctgcta aaaaacaatc
     1141 atataaaaac tgattatttt ctatgggaag tgaagctagc tgctctatag agcgagtaat
     1201 ttcttctgca atagtgcttc ctaaagacaa atcactaata atttcttgat aacgattgat
     1261 aatccaagtt tggtctttaa caatatcttt accgttaata taattttgat aatattgtat
     1321 caaagcatct gtcattttta agtcttcttc gtagcgtttc cctggagctg aaacgaca
//
LOCUS       DATRNK010000147         1352 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.148, whole genome shotgun sequence.
ACCESSION   DATRNK010000147 DATRNK010000000
VERSION     DATRNK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.148"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..1064)
                     /locus_tag="VBC52_002083"
     CDS             complement(<1..1064)
                     /locus_tag="VBC52_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEO6883626.1"
                     /translation="MHIIIQITIILLASVLATLISKRIGIPAVVGQLLVGIIIGPAML
                     GLVHQNQVLHVLSEIGVILLMFLAGLEANFDLLKKYLKPSLLVAITGVIVPMALFYFL
                     TRLFGFQINTAIFYGLVFAATSISITVEVLQEYNRVKTDTGAIILGAAVADDVLAVLL
                     LSVFIATNGSSSNIGLQIIIQLLFFVFLFICMKYLVPALFKLIEKVHFFEKYTILAIL
                     ICFSLSILADKVGMSSIIGSFFAGLAIGQTSFVDKVEHKISLLSYTFFIPIFFASIAL
                     PLKFDGMMSHLHTILIFTALAVLSKLIPGYFVGRGFNFSKLESLTIGGGMVSRGEMAL
                     IIVQVGLAAKIISSTTYSELV"
BASE COUNT          538 a          220 c          216 g          378 t
ORIGIN      
        1 acaagctcgg aataagtagt gcttgaaata attttagcag ctaatccaac ttgaactata
       61 atgagagcca tctcgccacg actgaccatt ccaccaccaa ttgttaaaga ttctaatttt
      121 gaaaaattaa agcctcgacc gacaaagtac cctggaatta attttgataa aacagccaaa
      181 gcagtaaaga ttaatatcgt gtgaaggtga gacatcattc catcgaattt aagaggtagt
      241 gcaattgaag cgaagaaaat tgggataaag aaggtgtaac taagaagtga aattttatgt
      301 tctaccttat caacaaaaga agtttgtcca atagctaatc ctgcaaaaaa cgaaccaatg
      361 atagaactca ttccaacttt atcggctaaa attgatagag aaaaacagat aaggatagca
      421 agaatagtat atttctcaaa gaaatgaacc ttttcgatta atttgaaaag agcaggtaca
      481 aggtatttca tacaaataaa taagaaaaca aagaataaca attgaatgat aatttgtaat
      541 ccgatattag aagagcttcc atttgttgca ataaagacac tcagaagcaa aacagctaag
      601 acatcatcag caacagcagc acctaaaata atagcaccag tatctgtttt tacacgattg
      661 tattcttgta aaacttcaac tgtgatagaa atactagttg cagcaaatac gagcccgtaa
      721 aagatagcag tattgatttg gaacccaaac aagcgtgtca agaagtagaa gagagccata
      781 ggaacaataa caccagtaat tgcgaccagt agactgggct taagatactt ctttaaaaga
      841 tcaaaattag cttcaagacc cgctaaaaac atgagtaaga taacaccaat ttctgacaag
      901 acatgtaata cctgattttg atgaacaagt cctagcattg caggtccgat gatgatacct
      961 acaagtagtt gtccaacaac agccggtatt ccaattcttt ttgaaataag agttgcaaga
     1021 acgcttgcca acaaaatgat tgtaatttga atgataatat gcattttttc ctcctaatat
     1081 taataaaatg atatcaatca tattgtactg aatatttaga ataccatttt ttaagcctaa
     1141 aatctaggaa tatttctttt ctgtcttttt catattatta tcaagaaaat ggaaaaatat
     1201 actgaaaaat attcaattta agaataatga acttgtaact atcgaactac ataaaaatga
     1261 tattttaagt gtaagtatct cttattagac ttcctgcgaa acaaaatatg gtacaatagt
     1321 tctatgaatt atgaagcaag caaacaatta ac
//
LOCUS       DATRNK010000148         1232 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.149, whole genome shotgun sequence.
ACCESSION   DATRNK010000148 DATRNK010000000
VERSION     DATRNK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.149"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..451)
                     /locus_tag="VBC52_002084"
     CDS             complement(<1..451)
                     /locus_tag="VBC52_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6883627.1"
                     /translation="MLYIFTPTFNRGYRLSYLYDSLCNQTNKNFIWLIVDDGSEDSTK
                     EIVSNYIKENKVSIVYLYKRNGGKHSAYNLAMRYMQPSDYHVCVDSDDWLLEDAVEII
                     FKDLESLTLSNRYVGLVYPRYSLNQGNNWLNPKILEVNIPDLKYKYHL"
     gene            complement(477..1202)
                     /locus_tag="VBC52_002085"
     CDS             complement(477..1202)
                     /locus_tag="VBC52_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEO6883628.1"
                     /translation="MIPKVIHYCWFGGNPLPDNLKKYIKTWREQCPDYEIIEWNEHNY
                     DVSKNVFMREAYTKKNFAYVSDYARLDIIYTYGGFYLDTDVELLKSLDPLRIHECFLA
                     REISCDVNTGLIIGAVKGHHFLKSNMSIYDKSDLTSLNKTCVEVTTNLLINRGLKNKN
                     IIQKIDDITIYPRNYFNPKNLLTGKVDCLTSVTYSIHHYEGSWKSSSFISDSLKIRVR
                     LIIDFLFGYGTYRMLLRFLKLKK"
BASE COUNT          432 a          230 c          127 g          443 t
ORIGIN      
        1 ttaagtgata tttatatttt aaatcaggaa tattaacttc taatattttg ggattcaacc
       61 aattattacc ttggtttaat gaatatcgag gataaacaag gcctacatat ctattagaca
      121 acgttaggct ctctaaatct ttaaaaataa tttcaactgc atcttctaat aaccaatcat
      181 cactatctac acatacatga taatcacttg gttgcatata tctcattgct agattatagg
      241 ctgaatgctt accgccatta cgtttataca gatatacaat actaacttta ttctctttta
      301 tataattact tacaatttcc ttagtactgt cctcagaacc atcatcaaca atcaaccata
      361 taaaattttt atttgtctga ttacatagag aatcatacag ataggataat cggtagcctc
      421 tgttaaatgt aggagtaaaa atatataaca tttcgtcctt tctattacaa taataactat
      481 ttctttaact ttagaaacct tagaagcatt ctataagtac catatccaaa taaaaaatca
      541 attatgagcc ttactctaat ctttagagaa tctgaaataa atgaagaact tttccaactt
      601 ccttcgtaat gatgtataga ataggtaaca ctagtcagac aatcaacctt acctgttaat
      661 aaattctttg gattaaaata atttctcgga tatattgtta tatcatcaat cttttgaata
      721 atattcttat tcttaagccc tctgtttatc aataaattag ttgtaacctc tacacatgtc
      781 ttattaagag aagttaaatc acttttgtca tatatagaca tatttgattt taaaaagtga
      841 tgtcctttaa cagcgccaat tattaatcct gtattcacat cacaactaat ctcccttgct
      901 agaaaacact catgaatcct caaaggatct aaacttttta aaagctccac atcagtatct
      961 agatagaacc ccccataagt ataaataata tccaatcttg catagtcaga aacataagca
     1021 aaattcttct tagtatatgc ttctctcata aaaacatttt tactaacatc ataattatgc
     1081 tcattccatt caataatttc ataatccgga cattgttctc tccaagtttt tatatatttc
     1141 tttaaattat ctggtaaggg atttcctcca aaccaacaat aatgtataac tttaggtatc
     1201 attttccttc cttacctcct gccatatcag ag
//
LOCUS       DATRNK010000149         1207 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.150, whole genome shotgun sequence.
ACCESSION   DATRNK010000149 DATRNK010000000
VERSION     DATRNK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.150"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            96..812
                     /locus_tag="VBC52_002086"
     CDS             96..812
                     /locus_tag="VBC52_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6883629.1"
                     /translation="MKKVIDLKKLQKAYASETVLNNINLEVFKGEIIGLIGPSGAGKS
                     TLIKTMLGMEKADKGTALVLDTQMPDRNILNQIGYMAQSDALYESLTGLENLLFFGKM
                     KGIQKTELKQQITHISKVVDLENQLDKFVSGYSGGMKRRLSLAIALLGNPTVLILDEP
                     TVGIDPSLRRKIWQELINIKDEGRSIFITTHVMDEAELTSKVALLLRGNIIAFDTPLH
                     LKKQFNVSTIEEVFLKAEGE"
     gene            815..>1207
                     /locus_tag="VBC52_002087"
     CDS             815..>1207
                     /locus_tag="VBC52_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6883630.1"
                     /translation="MRIIAITEKVIKELFRDKRTLAMMFLAPILIMFLMNVMFSANSN
                     TKVKIGTINVNTKVVSNLDNIKHIQVRSFKFNSSAKKALKSNKIDALISEDNKSYTVF
                     YANTDSSKTTLTRQAFKTAVNTMNSKELI"
BASE COUNT          449 a          184 c          203 g          371 t
ORIGIN      
        1 tcttttattg acaagcctgt ctttacacac tataatatat gatatcaaat aagtgagtaa
       61 acactcactc actgagagag gagcaaaaag ttatcatgaa aaaagtcatc gatttaaaaa
      121 aactacaaaa agcatatgcc tcagaaaccg ttttaaataa tattaatttg gaggtgttta
      181 aaggcgaaat aattggatta ataggaccct ctggagcagg gaaatctacc ttgattaaaa
      241 ctatgcttgg catggaaaaa gcagataagg gaacagctct tgttcttgat actcaaatgc
      301 cagatcgtaa tattttaaat caaattggct atatggctca atctgatgcc ttatacgagt
      361 ctttaactgg cttagaaaat ttattattct ttggaaaaat gaaaggtatt caaaaaactg
      421 aattaaaaca gcagataact catatttcta aagtagtaga tctagaaaac caacttgata
      481 aatttgtctc aggttactca ggaggtatga aaagacggct ttctctagcc atcgccctac
      541 ttggaaaccc cacagtttta atcctagatg aacctaccgt tggaattgat ccatccttga
      601 ggagaaaaat ctggcaagag ctaattaata ttaaggatga aggacgttct atctttatta
      661 caacccacgt tatggatgaa gcagaattaa caagtaaggt tgcactacta ttacgtggaa
      721 acattattgc ctttgatact ccattacatt taaaaaaaca atttaatgtg agtactattg
      781 aggaagtttt cttaaaagct gaaggagaat aatcatgaga attattgcaa taactgaaaa
      841 ggttataaaa gaactgtttc gtgataaaag aacacttgct atgatgtttt tagcacctat
      901 tttaattatg tttttgatga atgttatgtt ttctgcgaat agtaatacaa aagttaagat
      961 tggaactatt aacgttaaca cgaaggtcgt ttcaaattta gataatatta agcatattca
     1021 agtgagatca tttaaattta actcatctgc taaaaaagca ctcaaatcaa ataaaattga
     1081 tgctcttatt tcggaggaca ataaatctta tactgtcttc tatgcgaata cagattcttc
     1141 aaagacgact ttaacaagac aagcttttaa aaccgctgtt aatacaatga acagtaagga
     1201 actgatt
//
LOCUS       DATRNK010000150         1202 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.151, whole genome shotgun sequence.
ACCESSION   DATRNK010000150 DATRNK010000000
VERSION     DATRNK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.151"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            395..712
                     /locus_tag="VBC52_002088"
     CDS             395..712
                     /locus_tag="VBC52_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883631.1"
                     /translation="MTEEQEYKQYIYNNLIIQICESLNICSREFFIIKYNLSLDEINH
                     INNVFKEIVTKNILDYTIFKNKIQEGIPRFNSDVVFQTLLESYKSHQPIAQKILQMVC
                     KLW"
BASE COUNT          469 a          182 c          193 g          358 t
ORIGIN      
        1 cagaagattt aaaaatattt ccagtcttat catattcacc acataatagt gtatatgtaa
       61 aattaaattt acaaaaatgg taaaatgaaa ccaaaaacat ttcatttaaa atttatttag
      121 gttagaaagg agatactgcc aaaccctaga taaaaaacag aaacaaaatc gtcaatttag
      181 tcggtaaata tatcggtcga gatgcagctg tatctgtcgg agcatctctc aacaccgtta
      241 tgccggtctt gagaaattta ctgtactgga gcgatttagc ccacagaaca gttgaaggac
      301 aagtgaaaaa cgccttgaca acttccgaaa tggcagaaac aacagcttca accatagctt
      361 attggattcg tcttgcttta gagtaggggg cttaatgaca gaagaacagg aatataaaca
      421 gtatatttac aataatttaa taattcaaat atgtgaaagt ttaaacattt gttccagaga
      481 atttttcatc atcaaatata atttatctct agacgaaatc aatcacatca ataatgtttt
      541 caaagaaata gttacaaaaa acatcttaga ctacaccata tttaaaaaca aaatccaaga
      601 aggtattcca agatttaatt ccgacgttgt attccaaaca ttattagaat catataaatc
      661 ccatcaacct attgcacaaa aaatacttca aatggtatgc aagctatggt agttgctcca
      721 ataaaaaagg acaaaagctg aaccggatac tagtgaaata gtgattgctt atgcaggtac
      781 tatttcaggt ggtcgtttag acatagctac agatgttgaa atggttgctg gaggggatac
      841 atatttactt caggtgagaa atggaaatta cctgaagaaa ccacagtaaa taagaaagtt
      901 aaaaagataa ttacagaatt aactattact ccattaataa cagattatgg tgcaagacat
      961 acttatggga gtgttaaggt tcaagaagga gtagtatgga atctcctatt attgaggaat
     1021 ctgaattacc tctttttatt ataataaaaa gaggtaattt aattaattta aaagaagtta
     1081 tcaatatcaa ttgtatgtaa agatttagag ttttttaaga gctaacttca caaataaact
     1141 tccaaaaatc tatgacatta atcatggctt ttcttatgta tttatacgcc cctacagtca
     1201 aa
//
LOCUS       DATRNK010000151         1159 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.153, whole genome shotgun sequence.
ACCESSION   DATRNK010000151 DATRNK010000000
VERSION     DATRNK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.153"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..248
                     /locus_tag="VBC52_002089"
     CDS             <1..248
                     /locus_tag="VBC52_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6883632.1"
                     /translation="VAIARALVSDAPIILADEPTGNLDSVTAGEIINILKELAQDRNK
                     CVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE"
     gene            248..>1159
                     /locus_tag="VBC52_002090"
     CDS             248..>1159
                     /locus_tag="VBC52_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6883633.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMATLSIISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTNSIDSYVKRINSVADLV
                     DHDIIETQDTLANQSPERAKNFKRTVMLTGVNDSAKETKFVSEAYKLVEGKHLENKDK
                     NKILMHKDLAKKNNLKVGDKIKIKSNLFDADNEKVANETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDVHSAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLF"
BASE COUNT          484 a          143 c          210 g          322 t
ORIGIN      
        1 gggtagctat tgctagggca ctggtatcag atgccccaat aatactagct gatgagccta
       61 ccggtaacct agacagtgtt actgctggag aaataattaa tatattaaag gaattagctc
      121 aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca gattctgcgg
      181 atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg aatttggagg
      241 ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagcttaa aatcattaat
      301 tattatatta gttattctat caatggcaac tttaagcatc attagtttat ctattaaaga
      361 tgccacagat agagcttcta aggaaacatt tgctaatata acaaacagtt tttctatgga
      421 aataaataga caagtaaatc caggaacacc taggggtgga ggaaatgtaa aaggtgaaga
      481 tattaaaaag atatctcaaa caaacagtat agactcctat gtaaaaagaa taaacagcgt
      541 cgcagaccta gttgatcatg atataattga aactcaagat acacttgcaa atcaatcacc
      601 tgaaagagca aagaatttta agagaacagt tatgttaaca ggggttaatg actcagcaaa
      661 agaaacaaaa tttgtatcag aagcatataa gttagtagaa ggaaagcatt tagaaaataa
      721 agataaaaat aaaattttaa tgcataaaga tttggcaaaa aaaaacaatc tcaaagttgg
      781 agataaaata aaaataaaat ctaatttgtt tgatgctgac aatgaaaaag ttgcaaatga
      841 aactgtagaa gtagaaatta aaggtttatt tgatggtcat aatagcggtg gggtaagtgc
      901 agcacaagaa ctatacgaaa atacactaat cactgacgtt cattcagctg ctaaagttta
      961 tggtaataca gaagatacag ccgtatacca agatgcaaca ttctttgtaa aaggtgataa
     1021 gaatcttgat agtgtaataa aagatcttgg gaaactagat ataaattgga gagaatataa
     1081 tttgattaaa agttcatcaa attatcctgc gctgcaacaa tctatatccg gtatctattc
     1141 tatttcaaac aaattattt
//
LOCUS       DATRNK010000152         1124 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.154, whole genome shotgun sequence.
ACCESSION   DATRNK010000152 DATRNK010000000
VERSION     DATRNK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.154"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            95..1000
                     /locus_tag="VBC52_002091"
     CDS             95..1000
                     /locus_tag="VBC52_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6883634.1"
                     /translation="MKNQQKLMYLESIELYSNITKAAAHLFISQPYLSKVIKQLENEL
                     EIKLIQSQGHQTFLTYAGQRYLFYLKEIDMIERQMAKELYLIRSDKKGEITLGINSGL
                     ASSILANVLPKFNLEHPEISVKLLENNQNISEQLVASGDIDLAVGMAPILYKDGIAST
                     TIYRDELFLMIPTTSQLYNAEKRGQIIPFEYPISVLDNEPLILTPLEYGIGKTIAQFY
                     ELHHMSLNQMITTSTVPTAASLSLSGMGATFVPQTLIHRYLDKECNVYHFHKNKLFSE
                     YIMIYKKDVELSGIALLLYKAFLTK"
     gene            1061..>1124
                     /locus_tag="VBC52_002092"
     CDS             1061..>1124
                     /locus_tag="VBC52_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEO6883635.1"
                     /translation="MIYKIIASLFLVLIPIFSQVL"
BASE COUNT          389 a          212 c          164 g          359 t
ORIGIN      
        1 tagattgatt ttagctcaaa atgatagtaa acttatagcc ataacgtatc ataacttatt
       61 attaatctaa ggatatcaaa ctggttataa agtcttgaaa aatcaacaaa aattaatgta
      121 tctagagagt atcgagctat actcaaacat cactaaagcc gctgcacacc tttttatttc
      181 tcaaccttac ctaagcaaag tgatcaagca attagaaaat gagttagaaa taaaactcat
      241 ccagagccaa ggacaccaaa cctttttaac ttatgctgga caaagatatc tattctacct
      301 aaaagaaatt gatatgattg agcgtcaaat ggccaaagaa ctatatctca tccgttctga
      361 taaaaaagga gaaatcacac ttgggattaa ctctggctta gcaagttcta ttctggcaaa
      421 tgtacttcca aaatttaact tggagcaccc agaaatttct gtcaaattac tcgaaaacaa
      481 tcaaaatatt tctgaacaat tggtagcgag tggtgatatt gacctagctg ttgggatggc
      541 tcctatcctt tataaagatg gaattgcatc aaccactatt taccgcgacg aattgttttt
      601 aatgatacct accaccagtc aactctacaa cgctgaaaaa cgtggacaga ttattccttt
      661 tgaatatcct atttcagttc tagacaatga acctcttatc ctaacaccat tagagtacgg
      721 aattggcaaa acaattgcac aattctatga gcttcatcac atgtcattaa atcagatgat
      781 tacaactagt accgttccta cagcagctag cctatctttg tcagggatgg gagcaacatt
      841 tgttccacaa actctcattc atcgctattt ggataaagag tgcaatgtct accattttca
      901 taagaataag ttattttcag agtacattat gatttataaa aaagatgttg aattatctgg
      961 aattgctctc ctactttata aagctttttt aaccaagtag tttcgctaat tctattgaaa
     1021 atcagtttac atcttaccaa aatcggtata aaatagacct atgatataca aaattattgc
     1081 ttctttattt cttgttttaa tccctatatt ttcacaggtt cttg
//
LOCUS       DATRNK010000153         1078 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.155, whole genome shotgun sequence.
ACCESSION   DATRNK010000153 DATRNK010000000
VERSION     DATRNK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.155"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(142..933)
                     /locus_tag="VBC52_002093"
     CDS             complement(142..933)
                     /locus_tag="VBC52_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001918878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEO6883636.1"
                     /translation="MLVKNGEPFIQLDKEKKWFWHLDPMGQVSGPRSSGKTFFLYYLM
                     LSVAEIGADIYILDPKRSDFSSLKYCYPESERESHVATTPNSICKVLRELTELMNDRY
                     EKYFQISSSTLGFDAQKLGLRPIFIFFDEVLSLIEEDKKLGKEAEQYLKQIILKGRQS
                     GIYIIISSQRLSADVLNTVIRENCGLRVIFGKVQEESYRMALGESFKKLPRAEKGVGK
                     GYIYLDGQGWQTPKAFIAPYMDSSKMDIKQIFKNLLNNPPKVYKE"
BASE COUNT          330 a          193 c          154 g          401 t
ORIGIN      
        1 gcaatagtgc tacgcactat tggaagtccc taatttgtat agaatcggct ctgcctaaaa
       61 ccactccgtt ttcgtcaacg aggttgacga attttttcta tacagataag ggactgttat
      121 tgtgcgataa ttattactta attattcttt ataaacctta ggaggattat tcaatagatt
      181 tttaaaaatt tgtttaatat ccatcttcga actatccata tatggtgcaa taaatgcttt
      241 aggcgtttgc catccctgac cgtctagata aatatatcct tttccaacac ctttttcagc
      301 tctaggtaat tttttaaaag attctcctag agccattcta taggattctt cttgaacttt
      361 tccaaatatg actcttagtc cacaattttc tcggattact gtatttaaaa cgtcagcact
      421 cagcctttgt gaagaaatga taatgtaaat accagattgt ctacccttta aaataatttg
      481 tttgaggtat tgttctgcct cttttcctaa ttttttatct tcttcgatta gggatagtac
      541 ctcatcaaaa aaaataaaaa ttggacgtaa acctaatttt tgtgcatcaa agccaagggt
      601 gcttgaagaa atttgaaagt atttttcata tctatcattc attaattcag ttaattctct
      661 taaaactttg catatagaat ttggcgttgt tgcaacgtgt gattctcttt ctgattcagg
      721 atagcaatat ttgagagagg aaaaatcact acgttttgga tcaaggatat aaatatctgc
      781 tcctatttct gcaacactca acattaaata gtatagaaaa aatgtttttc cacttgagcg
      841 aggtccactt acttgtccca taggatccag atgccaaaac catttttttt ctttatctaa
      901 ttgtataaaa ggttcaccat ttttcactaa catcgattct atttccccca ttgtttttag
      961 aaagaacata taatgtaaaa ttaggcttac tatcgtctac ttcttcaacg tataagttta
     1021 atgcagtttc taaaattggt tgaagttcat tcattttatt agcaacactt aaaccatt
//
LOCUS       DATRNK010000154         1015 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.156, whole genome shotgun sequence.
ACCESSION   DATRNK010000154 DATRNK010000000
VERSION     DATRNK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.156"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..319)
                     /locus_tag="VBC52_002094"
     CDS             complement(<1..319)
                     /locus_tag="VBC52_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO6883637.1"
                     /translation="MKNNIIFSIEQYINDQEMPKGKQKVILSAIELFASQGFHGTSTA
                     QLAKNAEVSQATIYKYFETKDKLLVFILELIVQTIGRPFFTELSTFSTKEELIHFFVQ
                     DRFK"
     gene            complement(343..>1015)
                     /locus_tag="VBC52_002095"
     CDS             complement(343..>1015)
                     /locus_tag="VBC52_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6883638.1"
                     /translation="KLAQSLQTRSKYIKEKYNYGNKNTGFFAKMIPILMGFMVFFFVF
                     LISGMALLKERTSGTLDRLLATPVKRSDIVFGYMLSYGILAIIQTIVIVLSTIWLLDI
                     QVVGSIFSVIIVNFILALVALSLGILMSTLAKSEFQMMQFIPLIIMPQLFFSGIIPLE
                     NMASWAQTVGKILPLSYSGDALTKIIMYGQGLPNVSSNLLVLLLFLIILTIANIFGLK
                     RYRKV"
BASE COUNT          357 a          146 c          178 g          334 t
ORIGIN      
        1 atttaaaacg gtcctgtaca aagaaatgaa ttagttcctc tttagtagaa aatgtagata
       61 attctgtaaa gaaggggcgt ccaatagttt gtacgattaa ctcgagaata aagaccaaaa
      121 gcttgtcttt agtttcaaaa tatttatata tagttgcttg gctaacttct gcatttttag
      181 ctagttgagc agtggaagtt ccatggaagc cttgtgaggc aaagagctct atagcggata
      241 aaataacttt ttgttttcct tttggcatct cttggtcatt aatatactgt tcaatagaaa
      301 aaataatatt atttttcata cttagttcct tttcaaatgg tattatactt tacggtatcg
      361 ttttagtcca aagatatttg cgatggttaa aataattaaa aatagaagaa gtaccagaag
      421 atttgaagaa acgttaggta atccttgacc gtacataatt atttttgtaa gtgcatcacc
      481 agagtaggag agcggtagaa ttttaccgac tgtctgagcc caacttgcca tattttctag
      541 agggattatt cctgaaaaga ataattgagg cattataatt aagggaataa attgcatcat
      601 ttgaaattct gattttgcta gagtagacat taagattcct aatgataagg caaccagagc
      661 caaaataaaa ttgacaatga tgacactaaa aatactacct acaacttgaa tatctagtag
      721 ccaaatagtt gataaaacga taactatcgt ttgaataatt gctagaatgc cgtatgataa
      781 catatatcca aatacaatat cactacgttt aacaggagtt gcaaggagtc gatcaagtgt
      841 accagaagta cgctctttta ataaagccat tccagaaatt aaaaaaacaa agaagaagac
      901 cataaatccc attagtattg gtatcatttt tgcaaaaaag cctgtatttt tatttccgta
      961 attatatttt tctttgatat atttggagcg agtttgtaag gattgtgcta gtttc
//
LOCUS       DATRNK010000155          975 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.157, whole genome shotgun sequence.
ACCESSION   DATRNK010000155 DATRNK010000000
VERSION     DATRNK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.157"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            49..384
                     /locus_tag="VBC52_002096"
     CDS             49..384
                     /locus_tag="VBC52_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000827864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6883639.1"
                     /translation="MKRNKHLPLTETTYYILLALFEEAHGYAIMQKVEEMSGGDVRIA
                     AGTMYGAIENLLKQKWIKSIPSDDRRRKVYIITETGKEIVELETNRLRKLLNTANQLG
                     FGGDSYDKV"
     gene            371..>975
                     /locus_tag="VBC52_002097"
     CDS             371..>975
                     /locus_tag="VBC52_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEO6883640.1"
                     /translation="MIKFKLFFKSTEKEEEWIDKVQKSGYTFTGINSFLPAFYYFKRD
                     KEKDKMSIKMDFKKYISKDDYANYITLFEDSGWKHISGSRWGGLHYFQKIKPETSSEI
                     FSDQESKSAMAMSYYKYTCEHAFFSLFYFFLLIRTTSITIFDPKSWYLTQGLWEMQGK
                     IFWLAFLFETPFALLRILPMFYFLISGIYYITRAVKPKEII"
BASE COUNT          361 a          123 c          183 g          308 t
ORIGIN      
        1 attgtataga ataactatat aaagtttaac tgtatagaga gagaattgat gaaaaggaat
       61 aaacatttac cgttaacaga aactacctat tatatcttat tagctttgtt tgaggaagcg
      121 catggctatg ctattatgca aaaagttgaa gaaatgagtg gcggtgatgt tagaatagcc
      181 gcagggacaa tgtacggtgc cattgaaaat ttacttaaac aaaaatggat aaagtctatc
      241 ccaagtgacg atagaagaag aaaagtttat attattactg agacaggaaa agaaatagta
      301 gaacttgaaa cgaatcgatt aagaaagtta cttaatactg ctaatcagtt gggttttggg
      361 ggagatagtt atgataaagt ttaaattatt ttttaaatcc acagaaaaag aggaagaatg
      421 gatcgataag gttcaaaaaa gtggctatac ttttacaggc attaactcat ttcttccagc
      481 cttctattat tttaagagag ataaagaaaa agacaagatg tcaataaaaa tggattttaa
      541 aaaatacata tcgaaagatg actatgctaa ctatatcact ttatttgagg acagtggatg
      601 gaagcatatt agcggtagtc gttggggagg gctgcattat tttcaaaaaa taaagcctga
      661 aaccagttca gagatttttt ctgatcaaga atcaaaatca gcgatggcta tgagttatta
      721 taaatatact tgtgagcatg cgtttttctc tttattttat ttctttctct tgataagaac
      781 aacttctatc accatatttg atccaaaaag ttggtatctg acgcaaggtc tatgggaaat
      841 gcaagggaaa atattttggt tagcattctt atttgaaaca ccatttgcct tattaagaat
      901 attacctatg ttttactttt taatttctgg aatatactat attacacgag ctgtaaaacc
      961 caaggaaatt ataaa
//
LOCUS       DATRNK010000156          959 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.158, whole genome shotgun sequence.
ACCESSION   DATRNK010000156 DATRNK010000000
VERSION     DATRNK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.158"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..787)
                     /locus_tag="VBC52_002098"
     CDS             complement(<1..787)
                     /locus_tag="VBC52_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6883641.1"
                     /translation="MELISIIVPVYNGEIYIGRCLDSILEQTYQNLEIIIIDDGSSDR
                     TGDICEKYLLEDRRIKYFYQENRGQSVARNNGVLRCTGDWIAFLDSDDVYLPYSIEVM
                     YNIQKATNADIVLTSIGNFNNTYNTSINSQYLKEIKLYTLEVALEEMYYGKTYGVSPL
                     AKLYPRSNLLSNPYPEGKIHEDMDTTFKLISCASKIAVCDIVTAVVYFSDNSTTRTKF
                     NERMLYFFEAIQNNIVFINLNFPHNTSLISAVIYNEVFGGIDIC"
BASE COUNT          316 a          193 c          113 g          337 t
ORIGIN      
        1 cacatatatc tattccacca aatacttcat tatatattac agcagagatt aaagatgtat
       61 tatgtggaaa atttagattt ataaatacaa tattattttg aattgcctca aaaaaataca
      121 acattctctc attgaatttt gtcctagtag tactattatc gctaaaatac accactgcag
      181 tgacaatatc acaaactgct atttttgaag cgcaagatat aagcttaaaa gttgtatcca
      241 tatcttcatg aatttttcct tctggatatg gattactcaa taaattactt ctcggatata
      301 attttgctaa cggggaaact ccataagttt tcccataata catttcttcc aacgccacct
      361 ctaaagtata aagtttaatc tcttttaaat actgtgaatt aatactagta ttgtaagtat
      421 tgttgaagtt accaatacta gttaaaacaa tatcggcatt ggtagctttc tgtatgttgt
      481 acataacctc aattgaataa ggaagataaa catcatcact gtctaaaaaa gcaatccaat
      541 ctcctgtaca cctaagtact ccgttgttac gtgcaactga ttggcctctg ttttcttgat
      601 agaaatattt tattcgcctg tcttccaata aatacttttc gcaaatatcc cctgtgcgat
      661 cactagagcc atcatcaatt atgataatct ctaaattctg ataagtttgc tcaagaatac
      721 tatctagaca tctcccaata tatatttctc cattatatac aggcactatt attgaaatta
      781 attccattct ttactcgctc tttttaaacc ttgctacctt ccaaagatag ctaaaaccca
      841 acaaaatttt ccacccagcc tcttcactct ttagtgtagg tattacccct aaagtagtca
      901 ttttcaaagc ctgtatgttc atttttatct ttatagctga ccaccagcga tcaaatata
//
LOCUS       DATRNK010000157          955 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.159, whole genome shotgun sequence.
ACCESSION   DATRNK010000157 DATRNK010000000
VERSION     DATRNK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.159"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..802
                     /locus_tag="VBC52_002099"
     CDS             <1..802
                     /locus_tag="VBC52_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001909609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HEO6883642.1"
                     /translation="LSEQLDELLQVSTFMEKMGVKKEIDLSLFDNQSQRNLYFLNKGL
                     VLGEKVSSNYDESKLLHLRVANANFLTFYQFEKTNSGRLINIFLETPWCKKGDSIVDE
                     NAEDVSIFEVFEPNDWLKIDNCDFDAVIASYQRLVDTGNFVSSADETLIRIISASDLS
                     EDVGRKNLLLDWAQQLSNWNTTHSKGYERALINDLQIKIRRRPLNDKEKEKLNEILTS
                     NIEDSEVCFGVTVLLKSKSQADYYWNKLEEELQKRYQEFPIYKLYREL"
BASE COUNT          365 a          110 c          170 g          310 t
ORIGIN      
        1 tctaagtgag cagttagatg aacttcttca ggtttcaact tttatggaaa aaatgggagt
       61 gaaaaaggaa atagatttgt cactttttga taaccagtca cagagaaatt tatattttct
      121 taataaaggt cttgttttag gagaaaaagt ttcttcaaat tatgacgaat caaaattatt
      181 acatctaaga gttgctaatg caaattttct cactttctat caatttgaaa aaactaactc
      241 aggtagacta attaatatat ttttggaaac accttggtgt aaaaaagggg atagtatagt
      301 tgatgagaat gcagaagatg tttcgatttt tgaggttttt gagccaaatg attggttaaa
      361 gatagataat tgtgattttg atgcagtaat tgcctcatat cagagattag ttgacacagg
      421 aaactttgtt tcaagtgccg atgaaaccct tattagaatt atttctgctt cagatttatc
      481 agaagatgtg ggaagaaaga atttacttct tgattgggct cagcagcttt caaattggaa
      541 tacgacacat tccaaaggct atgaaagagc attgataaat gatttacaaa ttaaaatcag
      601 aagacgtcct ctgaatgata aagaaaaaga gaaattaaat gaaatattga ctagcaatat
      661 tgaagattct gaagtttgtt ttggtgtgac ggtcctactg aaatcaaaat ctcaagcaga
      721 ttattattgg aacaaattag aggaagaatt acagaaaaga tatcaggaat tccctattta
      781 taagttgtat cgagaacttt aattgttaga tatactattt taaaataatg ggaatagtcc
      841 aaaataggaa tgctataaca aaagctttaa gtatgatatt ttcaaatata tttaaaaatc
      901 attgaaccga tcgacttaat aagaaaattt ataaacaata aaaagaatcg tatta
//
LOCUS       DATRNK010000158          925 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.160, whole genome shotgun sequence.
ACCESSION   DATRNK010000158 DATRNK010000000
VERSION     DATRNK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.160"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..881
                     /locus_tag="VBC52_002100"
     rRNA            <1..881
                     /locus_tag="VBC52_002100"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          234 a          197 c          274 g          220 t
ORIGIN      
        1 agtacctgtg aagatgcagg ttacccgcga caggacggaa agaccccatg gagctttact
       61 gcagtttgat attgagtatc tgtaccacat gtacaggata ggtaggagcc tatgataacg
      121 ggacgccagt ttcgtttgag gcgttgttgg gatactaccc ttgtgttatg gctactctaa
      181 cccagatagg caatccctat cggagacagt gtctgacggg cagtttgact ggggcggtcg
      241 cctcctaaaa ggtaacggag gcgcccaaag gttccctcag actggttgga aatcagtcgt
      301 agagtgtaaa ggtataaggg agcttgactg cgagagctac aactcgagca gggacgaaag
      361 tcgggcttag tgatccggtg gttccgtatg gaagggccat cgctcaacgg ataaaagcta
      421 ccctggggat aacaggctta tctcccccaa gagttcacat cgacggggag gtttggcacc
      481 tcgatgtcgg ctcgtcgcat cctggggctg tagtcggtcc caagggttgg gctgttcgcc
      541 cattaaagcg gcacgcgagc tgggttcaga acgtcgtgag acagttcggt ccctatccgt
      601 cgcgggcgta ggaaatttga gaggatctgc tcctagtacg agaggaccag agtggactta
      661 ccgctggtgt accagttgtc ttgccaaagg catcgctggg tagctatgta gggaagggat
      721 aagcgctgaa agcatctaag tgcgaagccc acctcaagat gagatttccc atgattttat
      781 atcagtaaga gccctgagag atgatcaggt agataggtta ggagtgtaat tgtggtgaca
      841 catttagcgg actaatacta atagctcgag gacttatcca aaaagagata ttgacaacgt
      901 tacggtttct tgttagacta tagat
//
LOCUS       DATRNK010000159          815 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.164, whole genome shotgun sequence.
ACCESSION   DATRNK010000159 DATRNK010000000
VERSION     DATRNK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.164"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(255..338)
                     /locus_tag="VBC52_002101"
     tRNA            complement(255..338)
                     /locus_tag="VBC52_002101"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(302..304),aa:Leu,seq:caa)
     gene            complement(349..420)
                     /locus_tag="VBC52_002102"
     tRNA            complement(349..420)
                     /locus_tag="VBC52_002102"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(386..388),aa:Gln,seq:ttg)
     gene            complement(427..499)
                     /locus_tag="VBC52_002103"
     tRNA            complement(427..499)
                     /locus_tag="VBC52_002103"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(464..466),aa:His,seq:gtg)
     gene            complement(514..584)
                     /locus_tag="VBC52_002104"
     tRNA            complement(514..584)
                     /locus_tag="VBC52_002104"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(550..552),aa:Trp,seq:cca)
     gene            complement(591..671)
                     /locus_tag="VBC52_002105"
     tRNA            complement(591..671)
                     /locus_tag="VBC52_002105"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(635..637),aa:Tyr,seq:gta)
     gene            complement(683..755)
                     /locus_tag="VBC52_002106"
     tRNA            complement(683..755)
                     /locus_tag="VBC52_002106"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(720..722),aa:Phe,seq:gaa)
BASE COUNT          235 a          206 c          163 g          211 t
ORIGIN      
        1 ctacctatct atcattcaca aacactttta ttacttcaga acctatgaca tttaggagtc
       61 ctctttgaat ttcatttaaa tgttgagtct ccactaactc ttgaaaaatt tccttattat
      121 ttctgcttgt tttaaacctt ctataacgat tgcaataatg aaaaacaaat ataagtaatt
      181 ttcagtaact ttttctcaaa attaccagca caatacaaaa aagacaaggc ttctaaacct
      241 tgtctttata aatataccgg cggccggggt cgaaccggca cgtccgtgag gacactggat
      301 tttgagtcca gcgcgtctgc caattccgcc acgccggcta tcttaaaact ggggtagctg
      361 gattcgaacc aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca
      421 tgaaaaggcg agtgatggga atcgaaccca cgaatgtcag agccacaatc tgatgtgtta
      481 accacttcac cacacccgcc atattagaaa aaacacgggc agtaggaatc gaacccacac
      541 tgaaggtttt ggagacctta gttctacctt taaactatgc ccgtttacta tggagagaga
      601 gggattcgaa cccccgaacc cgaaggagcg gatttacagt ccgccgcgtt tagcctcttc
      661 gctatctctc ctaaggtata aatggcgcga gacggaatcg aaccgccgac acatggagct
      721 tcaatccatt gctctaccaa ctgagctacc gagcctattg cgggagcagg atttgaacct
      781 acgaccttcg ggttatgagc ccgacgagct accta
//
LOCUS       DATRNK010000160          802 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.165, whole genome shotgun sequence.
ACCESSION   DATRNK010000160 DATRNK010000000
VERSION     DATRNK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.165"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            2..>802
                     /locus_tag="VBC52_002107"
     CDS             join(2..376,379..>802)
                     /locus_tag="VBC52_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEO6883643.1"
                     /translation="MNYEASKQLTDVRFKRLVGVQRTTFEEMLAVLKTAYQRKHAKGG
                     RTPKLSLEDLLMATLQYMREYRTYEQIAADFGIHESNLIRRSQWVESTLIQSGFTISK
                     THLSAEDTVIVDATEVKINRPKKQLANYSGKKKCHAMKAQAIVTSQGRIVSLDIAVNY
                     CHDMKLFKMSRRNIGQAAKILADSGYQGIMKMYSQAQTPRKSSKLKPLTLEDKTYNHT
                     LSKERIKVENIFAKVKTFKIFSTTYRNRRKRFGLRMNLIAGMINRELG"
BASE COUNT          281 a          145 c          164 g          212 t
ORIGIN      
        1 tatgaattat gaagcaagca aacaattaac tgatgtacga tttaagcgcc ttgttggtgt
       61 tcagcgcact acttttgaag agatgttagc tgtgttaaaa acagcttatc aacgtaaaca
      121 cgcaaaaggt ggccgaaccc ctaagttaag cttagaagat ctcctcatgg ctactcttca
      181 atacatgcga gaataccgca cttatgaaca aattgcggct gattttggca ttcacgaaag
      241 caacttaatc cgtcgaagtc aatgggttga atcaactctt attcaaagtg gttttacgat
      301 ttcaaaaact catcttagtg ctgaggatac ggtgattgtg gatgcaacag aggtaaaaat
      361 caatcgtcct aaaaaaatca actagcgaat tattctggta aaaagaaatg tcatgctatg
      421 aaggctcagg cgattgtcac aagccaaggg agaattgttt ctttggatat tgcagtgaac
      481 tattgccacg atatgaagtt gttcaaaatg agtcgcagaa acatcggaca agctgctaaa
      541 atcttggcag acagtggtta tcaagggatc atgaagatgt attcacaagc gcaaactccg
      601 aggaaatcaa gcaaacttaa gccactaact cttgaagata aaacctataa ccatacgcta
      661 tccaaagaga gaatcaaggt tgagaatatt tttgccaaag taaaaacgtt taaaatattt
      721 tcaacaacct atcgaaatcg acgcaaacgg tttggattac gaatgaattt gattgctgga
      781 atgatcaacc gtgaactagg at
//
LOCUS       DATRNK010000161          759 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.166, whole genome shotgun sequence.
ACCESSION   DATRNK010000161 DATRNK010000000
VERSION     DATRNK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.166"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(83..>759)
                     /locus_tag="VBC52_002108"
     CDS             complement(83..>759)
                     /locus_tag="VBC52_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6883644.1"
                     /translation="DFDKFDNSEASFYATLARIRVMDREIKKFIRENPNSHILSIGCG
                     LDTRFERVDNGQIRWYNLDLPEVMEIRKLFFEEHERVTNIAKSALDETWTREVNPQNA
                     PFLIVSEGVLMFLKEDDVETFLHILTNSFSQFMAQFDLCQKEMINKGKQHDTVKYMDT
                     EFQFGITDGHEIVDLDPKLKQINLINFTDEMSKFELGTLRSLLPTIRKFNNCLGVYEY
                     KASEKK"
BASE COUNT          245 a          141 c           98 g          275 t
ORIGIN      
        1 tcctaaataa acaaagctac aaatgctata acgattatga caaatttaca tttagaatgt
       61 ctctttatat aaatagattt tgctactttt tctcagatgc tttatattcg tacacaccta
      121 aacaattatt aaatttacga attgttggaa gtaaagagcg aagtgtgcct aactcaaatt
      181 tgctcatctc atctgtaaag ttaatcagat ttatttgctt taatttaggg tctaaatcca
      241 caatttcatg accatctgtg ataccaaact gaaattctgt atccatatac tttactgtat
      301 catgttgctt tcctttatta atcatttcct tctgacacaa atcaaattgt gccataaatt
      361 ggctaaatga atttgtcagg atatgaagaa aagtctctac gtcatcttct tttagaaaca
      421 ttaaaacacc ttctgacacg attagaaaag gggcattttg gggatttacc tcccgtgtcc
      481 aagtttcatc tagggctgat tttgctatat tagtaactct ttcatgctct tcaaaaaata
      541 attttcttat ctccataacc tctggcaaat caaggttata ccacctaatt tgtccattat
      601 cgactctttc aaaccttgta tcaagtccac agccaattga aaggatatga ctatttggat
      661 tttctctaat aaattttttg atttctctat ccataacgcg aattctagct aatgttgcat
      721 aaaaagaagc ttctgaatta tcgaatttat caaaatcat
//
LOCUS       DATRNK010000162          749 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.167, whole genome shotgun sequence.
ACCESSION   DATRNK010000162 DATRNK010000000
VERSION     DATRNK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.167"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(<1..721)
                     /locus_tag="VBC52_002109"
     rRNA            complement(<1..721)
                     /locus_tag="VBC52_002109"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          165 a          231 c          139 g          214 t
ORIGIN      
        1 tcctccatat atctacgcat ttcaccgcta cacatggaat tccactctcc ccttctgcac
       61 tcaagtcctc cagtttccaa agcgtacaat ggttaagcca ctgcctttaa cttcagactt
      121 aaagaaccgc ctgcgctcgc tttacgccca ataaatccgg acaacgctcg ggacctacgt
      181 attaccgcgg ctgctggcac gtagttagcc gtccctttct ggttagttac cgtcacttgg
      241 tagattttcc actcctacca acgttcttct ctaacaacag agctttacga tccgaaaacc
      301 ttcttcactc acgcggcgtt gctcggtcag acttccgtcc attgccgaag attccctact
      361 gctgcctccc gtaggagtct gggccgtgtc tcagtcccag tgtggccgat caccctctca
      421 ggtcggctat gtatcgtcgc cttggtgagc ctttacctca ccaactagct aatacaacgc
      481 aggtccatct cacagtgaag caattgctcc ttttaaataa ctaacatgtg ttaattactc
      541 ttatgcggta ttagctatcg tttccaatag ttatcccccg ctatgaggca ggttacctac
      601 gcgttactca cccgttcgca actcatcagt ctagtgtaaa caccaaacct cagcgttcta
      661 cttgcatgta ttaggcacgc cgccagcgtt cgtcctgagc caggatcaaa ctctcattaa
      721 aagtttgagc tttgctcttt tctgtctcg
//
LOCUS       DATRNK010000163          682 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.168, whole genome shotgun sequence.
ACCESSION   DATRNK010000163 DATRNK010000000
VERSION     DATRNK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.168"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            88..>682
                     /locus_tag="VBC52_002110"
     CDS             88..>682
                     /locus_tag="VBC52_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0236 family protein"
                     /protein_id="HEO6883645.1"
                     /translation="MTFSRSRWTNGFETRIPVDEWLGLEKYKRYSIEFLYHVAKLATM
                     MPYRQVCKVIDSTLQTIITKDCVLKAVKFVEKLLKEKERYRFYLEEPPERKKVKKLYV
                     EGDGVMIKSTDSREERRYLDLTHFVIHTGSKKVSTKRYELQDKHEILQLNYDKAKYNL
                     LDYIYNNYEVDDDTILITNSDMGKGYTSRVFKELGKAL"
BASE COUNT          259 a           82 c          127 g          214 t
ORIGIN      
        1 ttaagtgtag aacactttat aatttctgtc tagtttagtg taacctattc acagtctgaa
       61 cgtacggttg ttttctcttt tggagaaata acatttagta ggagtcgctg gacaaatggc
      121 tttgaaacta gaataccagt agatgagtgg ttaggtcttg aaaaatataa gagatattca
      181 atagaattct tatatcatgt tgcaaaattg gctacaatga tgccttatcg tcaagtttgc
      241 aaagtaatag atagcacttt gcaaacaatc ataacaaaag actgtgtttt aaaagcagta
      301 aaatttgtag aaaaattgtt aaaagaaaaa gaacgctatc gtttttattt ggaagagcca
      361 cccgaacgta aaaaagtgaa aaaactgtat gttgagggtg atggagtcat gattaaaagc
      421 acagattcta gagaggaaag aaggtattta gatttaacac attttgttat tcatacaggc
      481 tcaaaaaaag tttctactaa aagatatgaa ttgcaggaca agcacgaaat attacagctt
      541 aattatgata aagctaaata taatctttta gattatattt ataataacta tgaagtagat
      601 gacgatacta ttttaatcac taactctgat atgggtaaag gctatactag tagagttttt
      661 aaggaattag gaaaagcact ta
//
LOCUS       DATRNK010000164          616 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.171, whole genome shotgun sequence.
ACCESSION   DATRNK010000164 DATRNK010000000
VERSION     DATRNK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.171"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(130..>616)
                     /locus_tag="VBC52_002111"
     CDS             complement(130..>616)
                     /locus_tag="VBC52_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6883646.1"
                     /translation="IELTDFALDAVKKYNSDKLELVFDTVESLICDELEDQEFQKFKK
                     KVLNNFKYIKPAHLRNLSNRGIGIMESQHRKITYRMKRRGMYWSKWGISTMANMIILE
                     RANGLRELFFGSWRKVYSEYKEGSFSAGRLFKKTDELDKFSKPLLKNGRKWSITGIKT
                     K"
BASE COUNT          199 a          109 c           70 g          238 t
ORIGIN      
        1 aattattctt aaaaagacca aaacaagtat tttttgcttt tctttaatat aattatacta
       61 ctttagaaga tttaatgcaa gttaaaacat gcaaaaaaac gctaaaataa aggtattttt
      121 agactttttc tattttgttt tgattcctgt tatactccat tttctgccat tttttagaag
      181 gggcttagaa aatttatcta attcatctgt ctttttaaaa agtcgccctg cactaaatga
      241 accttcttta tactcactgt atacctttct ccaagaaccg aaaaataatt ctcgtaaacc
      301 gttagctctt tcaagtataa tcatatttgc cattgtggag attccccact ttgaccaata
      361 catgccacgt cgcttcattc tatacgttat ctttctgtgt tgtgattcca tgataccaat
      421 accacgattt gaaagatttc taagatgagc tggttttata tatttgaaat tatttaatac
      481 ttttttctta aacttctgaa attcttgatc ttcaagttca tcacaaatca gtgattcaac
      541 agtatcaaaa actaattcaa gcttatcaga attatatttt tttaccgcat ctaaagcaaa
      601 atcggttaat tcaatt
//
LOCUS       DATRNK010000165          580 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.174, whole genome shotgun sequence.
ACCESSION   DATRNK010000165 DATRNK010000000
VERSION     DATRNK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.174"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..381
                     /locus_tag="VBC52_002112"
     CDS             <1..381
                     /locus_tag="VBC52_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEO6883647.1"
                     /translation="NLSYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWF
                     TDTYAHLSLQELKAKLNENINSIYAMIDLLSEEELFEAHMRKWADEATKTATWEVYKF
                     IHVNTVAPFGTFRTKIRKWKKIVL"
BASE COUNT          217 a           74 c          104 g          185 t
ORIGIN      
        1 aacctttctt atcaggttgg ctggaccaac ttggttctta aatgggaaga agatgaaaga
       61 aagggacttc aagtaaaaac accatcggat aaatttaaat ggaatcaact tggtgaatta
      121 tatcagtggt tcacagatac ctacgctcat ttatctctgc aagagttgaa agcaaaatta
      181 aatgaaaata ttaattctat ctatgcaatg attgatttgt tgagtgagga agaattattt
      241 gaagcgcata tgagaaagtg ggctgatgaa gcgactaaaa cagcgacttg ggaagtgtat
      301 aagtttattc atgtaaatac ggttgcacct tttggaactt tcagaactaa aatcagaaaa
      361 tggaagaaga tagtattata aattatattt ttaactttaa aaaatttcat aaaaatggtt
      421 accaaaggcg atagaagaaa aaactatcgt ctttttcttt ataaagtatt agatgaggaa
      481 aagaaatatt attttttcta atacaaatat taaattctca cgatagatgt aatatatctt
      541 gctatttctt caggctcttc agtaaagtca tgatctatcc
//
LOCUS       DATRNK010000166          578 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.175, whole genome shotgun sequence.
ACCESSION   DATRNK010000166 DATRNK010000000
VERSION     DATRNK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.175"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            1..578
                     /locus_tag="VBC52_002113"
                     /pseudo
     CDS             1..578
                     /locus_tag="VBC52_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646426.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
BASE COUNT          246 a           61 c          108 g          163 t
ORIGIN      
        1 agaaaagata taaaaattaa tcctgttaat aatcgaattc ctgtttcaga aatacaaaat
       61 ttgactaatt ggcataaagg atttgatttg ggttttccat tctgggaaga aggggaaaaa
      121 tatagaaaag aaggaaattt aaaacgatca attttgttat atgatttagc tagatctaat
      181 ggctattgtt cgcccgcact ttttgagtca tatgcaatgg cttttcatag attaaaagac
      241 tatgaaaatg aaattgatat cttagatgaa ggaatagagc gtgagggcaa tttaggtaga
      301 ttaattacac gtagaaataa tgcgatgaaa atgttaatca aatcaagaaa cgaacaaaag
      361 aaaataaaag aaaatgaaag tgctgaaaac atccttttta gtgaaataga gattttaaaa
      421 agaaaaaaac tagaaaaaat gcacgtccag tcttgaaaat ggacgatgaa atgaatatat
      481 tagaacattt tgagtctatt tcagatgcag taaaaaaaac aggaattaat tcaaaaagta
      541 tacgtgatgc ttcaaaaggt attcaaaagc atgcaggt
//
LOCUS       DATRNK010000167          495 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.180, whole genome shotgun sequence.
ACCESSION   DATRNK010000167 DATRNK010000000
VERSION     DATRNK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.180"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            <1..>495
                     /locus_tag="VBC52_002114"
     CDS             <1..>495
                     /locus_tag="VBC52_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO6883648.1"
                     /translation="EFIFASYKISKSKAFKNFCGRLLGREFIDSQHFKDINAYFLRHF
                     ICYCYYFIPDFYFLNTSRLSYSKDLYHLLDKGLADIFNLKGGNLTFSKHETVLLTMQL
                     SNLIETFLAPLSVYVISSSNIRLQTYQVMLNQYFTSKIAEFFFVNYQTTQIDEKLLKK
                     ADII"
BASE COUNT          168 a           65 c           69 g          193 t
ORIGIN      
        1 agaatttatt tttgcttctt ataagatatc aaagtcaaaa gcctttaaga atttttgtgg
       61 tcgattattg ggtagagaat ttattgactc gcagcatttt aaagatatta atgcctattt
      121 tttgagacat tttatttgtt attgttatta ttttattcct gatttttatt ttttaaatac
      181 atcaaggctg agctattcta aggatttata tcacctatta gataaaggtc tggctgatat
      241 ttttaacctg aagggaggga atttaacttt ttcaaaacat gagacagtgc tactaacaat
      301 gcaattatct aacttaatag agactttctt agctcctctt tctgtatatg taatttcatc
      361 tagtaatatt agattacaaa cctatcaagt aatgttaaac caatatttta catctaagat
      421 agcggaattc ttttttgtaa actatcaaac gactcagata gatgaaaaat tactaaaaaa
      481 agcagatatc attat
//
LOCUS       DATRNK010000168          358 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.denovo.190, whole genome shotgun sequence.
ACCESSION   DATRNK010000168 DATRNK010000000
VERSION     DATRNK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.denovo.190"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            complement(78..151)
                     /locus_tag="VBC52_002115"
     tRNA            complement(78..151)
                     /locus_tag="VBC52_002115"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Arg,seq:acg)
     gene            complement(163..247)
                     /locus_tag="VBC52_002116"
     tRNA            complement(163..247)
                     /locus_tag="VBC52_002116"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Leu,seq:taa)
     gene            complement(256..327)
                     /locus_tag="VBC52_002117"
     tRNA            complement(256..327)
                     /locus_tag="VBC52_002117"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(293..295),aa:Gly,seq:gcc)
BASE COUNT           99 a          101 c           76 g           82 t
ORIGIN      
        1 gaagacagga ttcgaacctg cgacaccttg gtcccaaacc aagtactcta ccaagctgag
       61 ctacttcccg aaaaatatgc accctagagg agtcgaacct ctaaccgcct gattcgtagt
      121 caggtactct atccagttga gctaagggtg ctaaatatta tatgccgagg accggaatcg
      181 aaccggtacg atgtttacca tcgcaggatt ttaagtcctg tgcgtctgcc agttccgcca
      241 ccccggcctc taacaagcga acgacggggt tcgaacccgc gaccctcacc ttggcaaggt
      301 gatgttctac cactgaacta cgttcgcact aaagacacta tttatcctat aaaaaatt
//
LOCUS       DATRNK010000169         4599 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699850-rid18550953.guided.099, whole genome shotgun sequence.
ACCESSION   DATRNK010000169 DATRNK010000000
VERSION     DATRNK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699850
            Sequence Read Archive: SRR7789245
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790110.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790110.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:21:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 2,041
            CDSs (with protein)               :: 2,041
            Genes (RNA)                       :: 35
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 8 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 3 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699850-rid18550953.guided.099"
                     /serotype="IV"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-03-07"
                     /collected_by="RMPRU"
     gene            40..1959
                     /gene="tet(M)"
                     /locus_tag="VBC52_002118"
     CDS             40..1959
                     /gene="tet(M)"
                     /locus_tag="VBC52_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO6883649.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2078..2245
                     /locus_tag="VBC52_002119"
     CDS             2078..2245
                     /locus_tag="VBC52_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO6883650.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2305..2658)
                     /locus_tag="VBC52_002120"
     CDS             complement(2305..2658)
                     /locus_tag="VBC52_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6883651.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            3163..3585
                     /locus_tag="VBC52_002121"
     CDS             3163..3585
                     /locus_tag="VBC52_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6883652.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            3582..3812
                     /locus_tag="VBC52_002122"
     CDS             3582..3812
                     /locus_tag="VBC52_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6883653.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            4273..4476
                     /locus_tag="VBC52_002123"
     CDS             4273..4476
                     /locus_tag="VBC52_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO6883654.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
BASE COUNT         1560 a          746 c          912 g         1381 t
ORIGIN      
        1 ttttatgccc ttttgggttt ttgaatggag gaaaatcaca tgaaaattat taatattgga
       61 gttttagctc atgttgatgc aggaaaaact accttaacag aaagcttatt atataacagt
      121 ggagcgatta cagaattagg aagcgtggac aaaggtacaa cgaggacgga taatacgctt
      181 ttagaacgtc agagaggaat tacaattcag acaggaataa cctcttttca gtgggaaaat
      241 acgaaggtga acatcataga cacgccagga catatggatt tcttagcaga agtatatcgt
      301 tcattatcag ttttagatgg ggcaattcta ctgatttctg caaaagatgg cgtacaagca
      361 caaactcgta tattatttca tgcacttagg aaaatgggga ttcccacaat cttttttatc
      421 aataagattg accaaaatgg aattgattta tcaacggttt atcaggatat taaagagaaa
      481 ctttctgccg aaattgtaat caaacagaag gtagaactgt atcctaatat gtgtgtgacg
      541 aactttaccg aatctgaaca atgggatacg gtaatagagg gaaacgatga ccttttagag
      601 aaatatatgt ccggtaaatc attagaagca ttggaactcg aacaagagga aagcataaga
      661 tttcagaatt gttctctgtt ccctctttat catggaagtg caaaaagtaa tatagggatt
      721 gataacctta tagaagttat tactaataaa ttttattcat caacacatcg aggtccgtct
      781 gaactttgcg gaaatgtttt caaaattgaa tatacaaaaa aaagacaacg tcttgcatat
      841 atacgccttt atagtggagt actacattta cgagattcgg ttagagtatc agaaaaagaa
      901 aaaataaaag ttacagaaat gtatacttca ataaatggtg aattatgtaa gattgataga
      961 gcttattctg gagaaattgt tattttgcaa aatgagtttt tgaagttaaa tagtgttctt
     1021 ggagatacaa aactattgcc acagagaaaa aagattgaaa atccgcaccc tctactacaa
     1081 acaactgttg aaccgagtaa acctgaacag agagaaatgt tgcttgatgc ccttttggaa
     1141 atctcagata gtgatccgct tctacgatat tacgtggatt ctacgacaca tgaaattata
     1201 ctttctttct tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat
     1261 catgtggaga tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat
     1321 gcagaatata ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta
     1381 tctgtatcac cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga
     1441 tacttaaatc aatcgtttca aaatgcagtt atggagggga tacgctatgg ctgtgaacaa
     1501 ggattgtatg gttggaatgt gacggactgt aaaatctgtt ttaagtatgg cttatactat
     1561 agccctgtta gtaccccagc agattttcgg atgcttgctc ctattgtatt ggaacaagtc
     1621 ttaaaaaaag ctggaacaga attgttagag ccatatctta gttttaaaat ttatgcgcca
     1681 caggaatatc tttcacgagc atacaacgat gctcctaaat attgtgcgaa catcgtagac
     1741 actcaattga aaaataatga ggtcattctt agtggagaaa tccctgctcg gtgtattcaa
     1801 gaatatcgta gtgatttaac tttctttaca aatggacgta gtgtttgttt aacagagtta
     1861 aaagggtacc atgttactac cggtgaacct gtttgccagc cccgtcgtcc aaatagtcgg
     1921 atagataaag tacgatatat gttcaataaa ataacttagt gtattttatg ttgttatata
     1981 aatatggttt cttgttaaat aagatgaaat attttttaat aaagatttga attaaagtgt
     2041 aaaggaggag atagttatta taaactacaa gtggatattg tgtcctgtat gtggaaataa
     2101 aacacgatta aagataaggg aagatactga attaaaaaaa ttccccctct attgtccgaa
     2161 atgcagacaa gaaaatttaa ttgaaataaa gcagttcaaa gtaactgtga ttacagagcc
     2221 agacgcaaag acgcagagcc gataaaatga gattaataca atctcatttt atcggctctt
     2281 tccgttatgt atggattctt ttaattagtc ttcgatgttt cttgcttcgt tgataccgct
     2341 ggctaaagat tccattaagg atagttcttt gtctgtaaag ctatccatgt atttctctat
     2401 ctgtaatcgt cgggtgcttt ttaccaagtt attagcaggt aagaaaaatt catcaacgga
     2461 aacatgaagt aacgatacaa ggtcataaag aacttgtatg ctggggtgtt gccctttatt
     2521 ttcaatatta gttaagtacc gtgggtcaat ttcaatcaat gctcccactt gttcacgagt
     2581 taaacctcgt ttcaatcgag cttctttaat ggctaaacca aaggctctaa aatcatattt
     2641 atcttctttt ttacgcatag tagaccacct ctatacattt tattgttcct actgaattaa
     2701 aaacaggtat agaaaaacgt gttatatggt ttataggttt atatttaata aaaagcacta
     2761 ctaaacgcca ataaaaaaaa ccgttatatg gtagtgctat ttacgctgtt aaaatattgt
     2821 atattacttc caaatggcgg tttgttggag gtcaacgtcg ccatgaagta catcatatac
     2881 aataaatttc cttacattgg gttcttgtca aaaaaagtcg tctatctgca atagataagt
     2941 acgtccacca atgtggtttt ataaatcata tagatagaat aacagaagca tgtaaacaga
     3001 gaaataaatc tgtttatatg cttttttggc tattcagaac ttttttacaa agtttattta
     3061 tcagtaatgc aacaaatccc cctttcacat tgggactaag agtgaaagga gataaacgag
     3121 caaggctcac ttcctttcct agacagaaag ggggtgagaa acatgaaacc atcttctttt
     3181 cagaccacaa tagaaaatca gtttgactat atctgtaaac gtgctatgga agacgagcga
     3241 aagaattata tgctttatct ttcaaggatt gcaaagcgtg aggtgtcctt ttcggatgtt
     3301 ggcgattatc ttgttagcca gtttgcgaca acagataact attcaactga ctttcagatt
     3361 tttacactca atgggttatc agtaggcgtt gaaaatgatt tgttgagtga agcattacgt
     3421 gagttgccag acaagaaacg tgaaattcta ctgctgtttt actttatgga catgagcgat
     3481 tcagaaattg cagacctgtt gaaattgaac cgttctactg tctatcggca tagaaccagt
     3541 ggactagcct taattaaaaa gtttatggag gaatttgaag aatgaaaaca caatatccta
     3601 tgattccctt tcctctcatt gtaaaggcaa cagatggcga taccgaagcg attaaccaga
     3661 ttctacatca ttacagaggg tacataacga agcgttccct acgacttatg aaagatgaat
     3721 atggcaatca aagtatggtc gttgatgaag tcttacgtgg aagaatggaa accagactga
     3781 ttacaaagat tttgtcattt gaaattaagt aatatcctct ctcctttcgt ggaagcgtgc
     3841 taaaccattc cacgcttccc gaacagggag gtttgttatt ccaccaaagc atattgagct
     3901 ttcaatgtgt tttgataggc taacgagcca ttgttctttg aaaactgaat aaaagtaatc
     3961 gaatacgttt cgataagaaa agagccaacg gaactaaccg ccatgaccta tcttataaag
     4021 atagcgagcg attcatgtta gtgatccgag aagcaatctt tagcaggatt gcctgcaacg
     4081 acattcttat cgtgataatg atactcccat acagtcaata gtccgagcgt gataaaaccg
     4141 tcgcaggcaa tgagtatggc tacatgagaa ccatgcaggg gtggaactcc cgtgagcttt
     4201 gctaaagctg ttcgattgct ggtaaaacaa cttttatgaa atccaaataa gtgatttgga
     4261 aaggaggatt ttatgaagca gactgacatt cctatttggg aacgttatac cctaaccatt
     4321 gaagaagcgt caaaatattt tcgtattggc gaaaacaagc tacgacgctt ggcagaggaa
     4381 aataaaaatg caaattggct gattatgaat ggcaatcgta ttcagattaa acgaaaacaa
     4441 tttgaaaaaa ttatagatac attggacgca atctagcgtc gccaaagggt cttgtatatg
     4501 ataaaatagt attaagtcgt atcaaggctc tttccataaa ggaaaggagc aaatgccatg
     4561 tcagaaaaaa gacgtgacaa taaaggtcga atcttaaag
//
